LOCUS       DAYYCW010000001       102065 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000001 DAYYCW010000000
VERSION     DAYYCW010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 102065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 102065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..102065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.001"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2145)
                     /locus_tag="ACHAYA_000001"
     CDS             complement(<1..2145)
                     /locus_tag="ACHAYA_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603843.1"
                     /translation="MSQWNTIDELRDIFIRKYTDSELSNELEKACSEEYELMRDYNGR
                     QILELLQNVDDAYGDIKSENDASNDEVKVQITYKNNILEVGNTGTSFSKDTIERLCLG
                     RASNKSSQNIGNKGTGFRSLLNDAEWVELYSGEFAIRFSEEFTKKLFEQYVSRDSDKF
                     SELIFEQKKNWKKEDYDLCFPIMNCPQPINKIDSGFDTLIRVKLKETNLSKGTSVSKQ
                     LQQPFYKSLLFLPNITKIVIETTDDEKISEKIVDGNDVLIEKSDVTGQSPSEEYFVFD
                     KESMIGDKVAKIALAVSKDSDYDYSKEKLYCYFPVRNFGTPIHALINAPFITNNSRDD
                     VPDDSEQINKKIFIEVLKFISEVAEKLSKPEYEDIALKMVTPFKENKLWDLDVFDLMN
                     EFLGILSNAQILPTVNWQYISIKDNPKLFLQEFPEELIGETFSTLLQLLETNENDQLV
                     QELANYNEYTGLSFVVDELSDKINILSSSWENRIRTKVFLWWSEQYKSSPIVPKLLKD
                     NSDNWLMKSDKVFLPTDTGVTVLPKELSSWVSLSILKQTYVDELIYQIKTSSSWKNKW
                     EETANAYKAERTGDKRILDAFSEKYLAIEFTEQSSSDLIIGTINRQIDTVEKSISFIN
                     WFYDNYNEKLSAGSELSKVPFNLPNVAGDVQPSNKMYIGKEYGNELGDKLFQDTDYTA
                     LKKIDCSSFAEERDFLSFILRCGVSKYPKIYDN"
     gene            complement(2268..3818)
                     /locus_tag="ACHAYA_000002"
     CDS             complement(2268..3818)
                     /locus_tag="ACHAYA_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8603844.1"
                     /translation="MAGKQVTVIPMKPRQWAAENTEEKPKLKVAAYCRVSTEMEEQAS
                     SYEAQVQHYTDYIQRNPDWELVGIFADEGISGTGTKKRDGFNRMIEACQNGEVEYIIT
                     KSISRFARNTVDCLQYIRQLKELKIAVFFEKENINTMDAKGEVLLTIMASLAQQESQS
                     LSQNTKMGVQYRFQQGQLRINHNHFLGYTKDEDGNLVIEPKEAEVIKRIFREYLEGSS
                     LQEIANGLMADGILTGGKRKLWRGEGVRLILRNEKYMGDALLQKTYTTDFLTKKRVKN
                     DGSYAQQYYVEDSHPAIIPKDIFMQAQQELDRRKSMKNKKSQCFSGKYALSGITVCGD
                     CGNAYRRVHWKNRGTVWRCKSRVDKREHNCTGRTIYETDLHGAVIKAINETLIDRDAF
                     LQQLNDNINSVMTDGLTDRLAELDSQLKELEAEIISLAVGGQGYDELASQIFSLRDER
                     DAVAKQIAANTNLQQRVDEMVVFVKEHDVINEYNEVLVRRLIEKVTIFEKNIVVDFKS
                     GVRVTVEI"
     gene            complement(3820..4185)
                     /locus_tag="ACHAYA_000003"
     CDS             complement(3820..4185)
                     /locus_tag="ACHAYA_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8603845.1"
                     /translation="MDEQQAQQIREFFNIYLEGVGLQEAGKRSGINKPHPFMKRILMN
                     EVYLGNEIYPKIIDEDIFFQADLEQERRIKFFKKRKEIKVPDTKVIPTHFKSGKITEK
                     YSDPFKQAQYAYSKIKEVK"
     gene            complement(4233..5786)
                     /locus_tag="ACHAYA_000004"
     CDS             complement(4233..5786)
                     /locus_tag="ACHAYA_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8603846.1"
                     /translation="MKTIHKLARPELPEASKLKVAAYARVSTSSNEQLASLQTQITHY
                     ENHIKNHEQWDYVGVYYDEGISGTKTVKRDGLKRLIKDAELGKIDLILTKSISRFSRN
                     TVDCLNLVRQLTDIGVTIFFEKENINTGDMESELLLSILSSLAESESYSHSENLKWAI
                     RNRMSKGKFKAYPPYGYKRKGYDFHIVAAEAKVVKQMFQWALDGISTGMIAKRLNDGK
                     QFTKRGANWQATSVRSILKNEVYTGTLTMQQTFVDDQYKRRINKGELPMYQIKNNHEP
                     IVSKEDYDRVQELIRRKAEAKGNTGDGKKYTKRYAFSGNIICDKCGSKFKRVHTYWKG
                     NTKKVQWKCTGHLKNKDSCDALPITDEDLKVAYLTMLNKLIFGHKLVLEPFMKSLNKA
                     GANQEGLEVLADEIDKIQEKLEVLASLTASSILSTKVSLEEQSKLQQERAVAQEKQRL
                     IIESVNGKSTQQIELEALYKFARMAEMLTDWDEDLFKKFTNSITVYNRQEVGFELKCG
                     LLLKERLPE"
     gene            complement(5847..6020)
                     /locus_tag="ACHAYA_000005"
     CDS             complement(5847..6020)
                     /locus_tag="ACHAYA_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFM8603847.1"
                     /translation="MTNKKTEINYSDEAAYYISQQILLNLLVTGKINLEEYVKIDNKN
                     RETFNPFLSRIMV"
     gene            6315..6620
                     /locus_tag="ACHAYA_000006"
     CDS             6315..6620
                     /locus_tag="ACHAYA_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603848.1"
                     /translation="MSSFDMEGLEKEYEHFCKSATTKELEEFDESFKQEFEKCFKTIE
                     AEEALYQFLQANINAMNNRMESIESPLFCINYKEQVFQLRNHLILMQNAVNTYLKEN"
     gene            complement(6681..7718)
                     /locus_tag="ACHAYA_000007"
     CDS             complement(6681..7718)
                     /locus_tag="ACHAYA_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFM8603849.1"
                     /translation="MVTFKDLLPEPTESQQAFILDHGKTEDDGQLKYADDAKSYGWNM
                     RQYGKLKAGAVVLNRHPGKITKDRKWEIYGGGYVESISEPDEAGNVTAVITHAFTIEP
                     AIKQGDSFIENFDWNTPSKKKRKKPNSWAYFWDQYGMNEITYADFVALIENRHLIPID
                     DTQSLPSELDLTNEEVEEIEQESSKGFTVLVDEVGPNRTNGTQKRKFTGRHTDWERVN
                     KAKQKTGALGEEIVLDFLIQEAEKNKTKLPEHVSKTEGDGHGYDICAFDQSGNEIHIE
                     VKASKTNFSDGFEMSTNEVAASLGDTPYKIYFVHDLDVASKVCKIKIYDGPITEENFT
                     MVPTSYKIFNK"
     gene            complement(7834..8742)
                     /locus_tag="ACHAYA_000008"
     CDS             complement(7834..8742)
                     /locus_tag="ACHAYA_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8603850.1"
                     /translation="MGISNLATKYGFQSFSHYSNGRSGAKVDKIVIHHMATTNYDIVP
                     DIWKTREASAHYGIGKNGEIRAYVDEGNTAWHAGNWNANISSIGIEHCNSTGSPNWGI
                     NQATIDASAKLCADIAKRYGLGKLVVNKNIFPHSHFSATSCPGQLLGKLQEIADKANA
                     INNGSSTPSTPASGLYRVRKSWADAKSQIGAYKDLNNAKRAADSHSGYKVFDQNGNQV
                     YPSGSSTSNVKTLVVQVSGLNVRNKPSLSGAVVGSYNKGATWTLPKSEKMTIADGWVW
                     ARCPIGYCAVGKNTGKTESDDYILIS"
     gene            complement(8732..9151)
                     /locus_tag="ACHAYA_000009"
     CDS             complement(8732..9151)
                     /locus_tag="ACHAYA_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin family protein"
                     /protein_id="HFM8603851.1"
                     /translation="MKEVWNWIQLAIAGGGGVIGWYVGGIDGLIYTLVTFVVVDYVTG
                     LLRAINEKKLSSKIGAKGITKKIMIFLLVGVAHMLDVEVIKGGNVLRDAVIFFYISNE
                     GISLLENAVAIGLPVPEKLREILQQLHEKKEETENGN"
     gene            complement(9152..9559)
                     /locus_tag="ACHAYA_000010"
     CDS             complement(9152..9559)
                     /locus_tag="ACHAYA_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HFM8603852.1"
                     /translation="MLKIRNEDLVDVMSFLKGFDLPPKISRVRTKLAKLIQVKVDELY
                     QDEVDLLQKYGKKDEHGNLVQHDGAFSLIPETATEYHKEKADLLNEETHINVDELQDK
                     LPLLVAGFENSEVTISGKDAEMLDLILEQLEDL"
     gene            complement(9546..9713)
                     /locus_tag="ACHAYA_000011"
     CDS             complement(9546..9713)
                     /locus_tag="ACHAYA_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603853.1"
                     /translation="MNEEKEEVIVTEPIEADIPKPMKVGTDMVDLLVMVVDDLCVQIG
                     DLKEKIDNAQN"
     gene            complement(9723..13613)
                     /locus_tag="ACHAYA_000012"
     CDS             complement(9723..13613)
                     /locus_tag="ACHAYA_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8603854.1"
                     /translation="MITLYNKTESNFNHNGLAVLDGNILNPVVSEELNGLFSFEFDYP
                     IHAPHSEELLPEMIVKAPVPELSDQLFRIVERDDALGGFLHIVAHHIFYDLATNFIED
                     TYIVNKNGSGALTQLLGATQFMHRFTGTSNISAVNNVRLVRLNPVEVLLDSDLENGFQ
                     SSYGGEIVRDNFTISMLTTRGSNNGVQIRDKKNLTGYKADVDYSGIVTRIMPQGYDGL
                     FLPEKYVDSPNINQYVQPKIKVIKYDNVKVGDEEDEFTQAEAYDELRRLAGLEYSANK
                     VDLPKATYDVEFAPLERTEEYKDFAQLETINLGDTVSVIHAEDGLNITARMVSYKYDP
                     FLKAYISVTLGNITPKFTDVAKDINRVDNKAEQAKDDANYALSSANGKNTNFYGPTTP
                     NNPKLGDVWYKENGDKFEMWVYEIRDGVTQWYPLMTDMTAEEVKQAVEQAQKDANTAT
                     EKANQAFNDAVNALNAAESVGASVETLTTSVEQNTSKISLVASGMSTLEGRVITAEST
                     LTVQAGQIASKASQSTVDNLTGRITTAESTITQNADRFELSLSKTNKAVNVLAGMSIA
                     DLQWAVGGLNTNDGKENTYGGYVRSGFTRVRNGEKYLLQTFVGGKLYSTYATAYLLYY
                     KEDKSFLSYTQFGNTTTPLTVPSSAAFIRIRLTTSDAPETISCYMLKTDVTNGYVDLS
                     EISNVVKLTATTDALLLSVSETKEAIGVPFKVNQWEQGSLSTTDGSEVSATNYLRSEF
                     LDVKVGDKYIGQLRDGTQAAFYYHYYGMDLPYIDFVPTAKQFSETLTAGTYTNATIAS
                     YLVSKNVESMSITSGVTTNPYSNSGDYLVIYKLPLEGKVAPSVISWNGRKDTSDNIVL
                     LYTGGAWEQISTVTSSSNATYSWTLTAEQISGITTDSVYLAFFSVKTGTYAGIYNSSN
                     TPFTLNPSDGSSYHQISYTGSSGAITVPSGTLKMRVRVNTSIKPDEYEGNIYPTTTRQ
                     DYTNANTLYSAILMQKDLINLRVGKGDVINQINISPESILIAGNKVHITGQTTIDSAV
                     ISSAMIQNAAITNAKIADATIQSAKIASIDAAKITTGTLSAARIGANSITADKIAANI
                     LTALTASSSIRITGTTIGYYSGSTLVTEINSQGMTIRRGGTTVGTIGANNISGHSDWR
                     GLVFDLEYGTEYMSWAHKDSSSASVYTQKLSWYARTLQSGMEKGFHFDDDIYLPSMLR
                     IGTQHQISVGGITFSSSLYTKIGSSSGSAGIAFNGSNLMLGDDGKWVDFGVIREICAK
                     LAGRTIALPTGFSSNGTASGWYNPQSFNSMTTYS"
     gene            complement(13610..14302)
                     /locus_tag="ACHAYA_000013"
     CDS             complement(13610..14302)
                     /locus_tag="ACHAYA_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8603855.1"
                     /translation="MNAFILDGTINSRTDLGLRITEVPVIPVTERVIDSIEIDGREGD
                     LTILKGWKDVTFGFKVVIWKSDVWTSWQIILPQILNAKTIAFSNEPNVFYPIKYAKAS
                     GLTQILSSMWEFEMELTCSPFRYKTNVATINRTTSGTVNNPGNIYSLPKIKVYGNGTR
                     TLTINGKPIVLNLTNGNLTLDSDLKECFYGDVAANQYMTGDFPEFRVGSNTVTLGTGI
                     TKVEIEPRWRYL"
     gene            complement(14379..14780)
                     /locus_tag="ACHAYA_000014"
     CDS             complement(14379..14780)
                     /locus_tag="ACHAYA_000014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603856.1"
                     /translation="MLKHKFKAKKPALIGYILLLAIGLFLTAVRWYSGINHGFVFINN
                     QINSHISNFALSFLFYLVIGYLWLLMGTNLRVLTAMGAGLIVANIVCETMMTFLNTPD
                     LIDAIYGISGVLVVYAYLAITKKIGLEETTE"
     gene            complement(14851..16911)
                     /locus_tag="ACHAYA_000015"
     CDS             complement(14851..16911)
                     /locus_tag="ACHAYA_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8603857.1"
                     /translation="MGTVALAAGAAAVELGKQVVSAYADYEQLVGGVDTLFKDASSTV
                     QKNADNAFKTAGMSANEYMETVTGFSAALISSLGGDTKKAAGVADQAVTDMSDNANKM
                     GTDIESIQNAYQGFAKQNYGMLDNLKLGFGGTKSEMERLLQEAEKISGVKYDISNFAD
                     VTEAIHVIQTEMGITGTTAKEASETISGSIDGMKSAISNLMAGLGNSSADIGMLIGNV
                     VEQFQNVIKNILPVIQNIVSALPAVVDAIIQALGQLLPTLLEAVTSLFSQVLETILGL
                     LPTLIPVVVQAVLTIVQTLIENLPLLIDAAFQLVTTLIQGLGEALPELIPITVQAIIT
                     IVNGLIENLPLLLDAALQLIMGLAQGLITALPILIESLPQIITGIVNFIISAIPQIIQ
                     TGIQLLTSLVTALPDIINAIVAAIPVIIQNIINAVIGAIPQLIDAGIQLLVALIGALP
                     QIITTIANAMPQIINGITGTLVNNIDKIINAGVKLLVALVQNLPAIISAIVKAVPQII
                     TGLANAFGEYVSTMGTIGKNLITGLWNGIADAGSWLRNKISGFFGGVVDNIKDFFGIH
                     SPSTLFRDEIGKYMAQGIGVGFDKEMTGVSKTMQKAIPTKLYMPSFDIDTDIHAAVDS
                     TFSMLDLGAKVDALTNITSEMLPTLLKAMDIKVVLNDGTLVGKLAPEIDKSLATLNRR
                     RVAY"
     gene            complement(17431..17571)
                     /locus_tag="ACHAYA_000016"
     CDS             complement(17431..17571)
                     /locus_tag="ACHAYA_000016"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603858.1"
                     /translation="MGMTSDDFWYCSLGLFLDLWECHKQFTGISKPKVELFIDDVIPS
                     GI"
     gene            complement(17619..17999)
                     /locus_tag="ACHAYA_000017"
     CDS             complement(17619..17999)
                     /locus_tag="ACHAYA_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603859.1"
                     /translation="MSEERSAKISLGGDEYELILTTKATKEIASHYGGLDNLGEKLLK
                     SENFELALDEIIWLITLLANQSIKIYNLKNTEKKDELTTEYVELMTSPLQLAEYKSAI
                     TEAMFKGTARNIESETDSKNKAGE"
     gene            complement(18009..18599)
                     /locus_tag="ACHAYA_000018"
     CDS             complement(18009..18599)
                     /locus_tag="ACHAYA_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8603860.1"
                     /translation="MATIGLDKLYYAPITESATTGEETYGTPAMLAKAISAELSVELA
                     EATLYADDGASEVVKEFKNGKLTLGVDDIGRAVAAVLVGATVDQNGVLISTTEDGGTP
                     VAIGFRARKSNGKYKYLWLYRVKFAVPSTSLATKGDDITFSTPTIEGTVMRRNKLDAS
                     GKHPWKAEVDENDTGVEQTTINGWYTSVYEPDFTAD"
     gene            complement(18602..18943)
                     /locus_tag="ACHAYA_000019"
     CDS             complement(18602..18943)
                     /locus_tag="ACHAYA_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603861.1"
                     /translation="MNILEELNSLFATLHIPVETGEFSDKAPDKYCVLTPLADRFDVY
                     GDNFPTVDVQEVRISLFVKGNYLNTQKQLVQNLLKHEFTITERLFVGFEKDTNYYHVA
                     IDVAKHYEMEV"
     gene            complement(18940..19371)
                     /locus_tag="ACHAYA_000020"
     CDS             complement(18940..19371)
                     /locus_tag="ACHAYA_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8603862.1"
                     /translation="MAKALMKMPEDFLLKVSKLENKTDEIIPKVLESGAEVVEGKVRS
                     NLTAVVGSNTKVPSRSTGELESALGITQARQDKSGNWNIKVGFDEPRIDGSSNAKIAN
                     ILEYGKHGQPAKPFLKPAKSQSRKSCIETMKSKMESEVNGI"
     gene            complement(19364..19702)
                     /locus_tag="ACHAYA_000021"
     CDS             complement(19364..19702)
                     /locus_tag="ACHAYA_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8603863.1"
                     /translation="MSFGKMNQRIQIVKVTNQKDSAGFSVKQDEVLAAVHAYKEEKNA
                     TEKWVNMATFSSATCLFRIRYIPNLEVTTEMVVISDQKRYQIVSVENVRNRNMYLEML
                     TTEMEVSADG"
     gene            complement(19699..20010)
                     /locus_tag="ACHAYA_000022"
     CDS             complement(19699..20010)
                     /locus_tag="ACHAYA_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8603864.1"
                     /translation="MSDLLNKVKANLILEHNEDDELLERLILTALSYAESYQHIESGY
                     YSEHEMPPTTEQAVIVLASNFYESRDGSTGGFFADNVNASKQVWETVNLLLRLDRRWQ
                     V"
     gene            complement(20021..21232)
                     /locus_tag="ACHAYA_000023"
     CDS             complement(20021..21232)
                     /locus_tag="ACHAYA_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8603865.1"
                     /translation="MNKIQELMEKRAQAWNGAKAFVEAKQDKDGLMSEEDAKTYAEME
                     TKVKNFTMEIERMQSMEAMDRELEKPMSQPLTSQPMTTENKMEPKKTGRASAEYKEGV
                     LEALRSNFRHVSNVLQEGVDTAGGYLVPEEYDSRLIDGMTSENIMRTLATTITTSGEH
                     KINIAGSKPAASWIEEGGELSFGDATFDQILLDAHKLHVAIKVTDELLYDNAFNLENY
                     IIDQFTKALANAEEDAFLNGDGVGKPLGIFAATGGGQVAITTNTQSAVTADDVVNLVY
                     ALKRPYRKNAKFIMNDQTISLLRKLKDNNGAYMWQPALVAGEPDRLLGYPVYTSEFVP
                     TVKAGAPVIAFGDFSYYNIGDRGVRSFDQLRELFAGNGMVGFLAKERVDGKLVLPEAV
                     QILKMKASASS"
     gene            complement(21236..21991)
                     /locus_tag="ACHAYA_000024"
     CDS             complement(21236..21991)
                     /locus_tag="ACHAYA_000024"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8603866.1"
                     /translation="MKKFWKWKNQTVLNQDNQEEILERTLYLNGPIAEESWFDDEVTP
                     QIFKDELNSGGGNVTVWINSGGGDCVAAASIYNMLADYKGKVTVKIDGIAASAASVIA
                     MAGDKVLMSPVAMVMIHNPSAVAFGEKVDMEKAIAMLDEVKASIINAYERKTNLSRVQ
                     LAHMMDAETWMDANKAVHLGFADEIIGEIDPDIPVNNSMYSEVKVTNSLTSAIAKKCR
                     IEPPEPKEREEINNTIPADSLMSRLNLMKNWRN"
     gene            complement(21891..23219)
                     /locus_tag="ACHAYA_000025"
     CDS             complement(21891..23219)
                     /locus_tag="ACHAYA_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8603867.1"
                     /translation="MGIIKSLFKSRDKPTNQTIMNPYRFMFGGSSAGKSVTEQTAMQM
                     TAVYSCVRVLSEAVAGLPLHFYQYRDAGGKDKAMKHSLYFLLHDEPNSEMTSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVVALYPLMPDKMTVNRDDNKEIYYLYQADVGQVRLS
                     KADVLHIPGLGFDGLVGYSPIAMAKNAIGLAIATEEYGSKFFANGANPGGVLEHPGTL
                     KDPGKLRESWNATFGGSSNAAKVAVLEEGMKYTPISMSPEQAQFLETRKFQLEEIARI
                     FRVPLHMVGDLDHATFSNVEHLSLDFVKFSLSPWLNRWEQALNKSLLSRDEKEKYFIK
                     FNVDGLLRGDYATRQQGYATGIQNGFLSPDDVRELEDLNPIPGGAGAKYYFNGNMLPI
                     EQAGAAYGVNKEESEEQDEEVLEVEESNGSESRQPRGNPRENPLPQRTNR"
     gene            complement(23285..24019)
                     /locus_tag="ACHAYA_000026"
     CDS             complement(23285..24019)
                     /locus_tag="ACHAYA_000026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603868.1"
                     /translation="MNEYLIQVAPDKISSDPDNTLISRLFYRKWWGKILTEDLTKYAE
                     GIGNYRGETIISFNTIAGATIRLLKCYNQKNFSMPDSHYGRLKVILYSDDITEEMKVK
                     FVKFYDLSHTLANFMPLYVSKPSLNGVKGKGPYHDFPDLFYSDVRDKRYGSSTNADKF
                     IVNNKDYFSKFKDWKSFIELNYLQDFFDDEDYMTFKKLAPSSSFFPYQRKSLTPDEKS
                     KCLEEVNTFLNNAIEIILSRGSRFYI"
     gene            complement(24075..25640)
                     /locus_tag="ACHAYA_000027"
     CDS             complement(24075..25640)
                     /locus_tag="ACHAYA_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8603869.1"
                     /translation="MDKDSHYDKSRADYAVAFIELLKHTKGQWFGKPFKLMPWQEKII
                     RDLFGTIKANGYRQFNTAYVEIPKKNGKSELAAAVALLLTCGDGEERAEVYGCAADRA
                     QASIVFDVAVDMIRQNPALSKRVKILTSQKRIIYQPTNSFYQVLSAEAYSKHGLNSSA
                     VVFDELHAQPNRKLFDVMTKGAGDARTQPLFFLITTAGNDTNSICYEQHQKALDIIEG
                     RKHDKTFYPLIYGIKQDDDWTDPEVWKKANPSLGITIAEEKVVAACDSAKQNPAEENS
                     FRQLRLNQWVKQAVRWMPMDKWDACSFNADEESLRGRVCYGGLDLSSTTDLTSFVLVF
                     PPEDEDDKFVILPYYWVPEDTLDLRVKRDHVNYDLWAKQGYIQTTEGNVVHYGFIEKF
                     IEELGEKYNIREIAFDRWGAVQMVQNLEGMGFTVVPFGQGFKDMSPATKELMKLTLEQ
                     KLAHGGHPVLRWNMDNIFVRTDPAGNIKADKEKSTEKIDGAIATIMALDRAIRCGNDN
                     GASVYDDRGLLFL"
     gene            complement(25723..25866)
                     /locus_tag="ACHAYA_000028"
     CDS             complement(25723..25866)
                     /locus_tag="ACHAYA_000028"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603870.1"
                     /translation="MMDYQIYMPDNEDGIEEGYYDWQELVQLLRDNKNNPEAIQFIAD
                     MME"
     gene            complement(25863..26039)
                     /locus_tag="ACHAYA_000029"
     CDS             complement(25863..26039)
                     /locus_tag="ACHAYA_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HFM8603871.1"
                     /translation="MNAKIKQQIIEIAESGLTNMFDAIAVQRIAHDRHFYELVIFIEE
                     HKDEYSRFILEGDK"
     gene            complement(26023..26217)
                     /locus_tag="ACHAYA_000030"
     CDS             complement(26023..26217)
                     /locus_tag="ACHAYA_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603872.1"
                     /translation="MKVSKRKIYNIAKNYIVQLPERGDLKAHNSDREDFLDIAVWCLE
                     DALIAAYEAGRKDGQNERKD"
     gene            complement(26219..26596)
                     /locus_tag="ACHAYA_000031"
     CDS             complement(26219..26596)
                     /locus_tag="ACHAYA_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HFM8603873.1"
                     /translation="MFPTHEQIEGMKKRYPEGSRVELIKMNDPHAPAKGTQGTVTGVD
                     DIGSILIQWDNGSTLNAVYGEDVVHIIKPDFELVFQNGRREGFETFNDAWQYVTDMIL
                     NHDLVWVDLESKTEDTIYIRKGL"
     gene            complement(26670..27905)
                     /locus_tag="ACHAYA_000032"
     CDS             complement(26670..27905)
                     /locus_tag="ACHAYA_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFM8603874.1"
                     /translation="MNTTERFEKVDIDKLVPYARNARTHSKDQVKQIQASMREFGFIN
                     PVIVDKDYNLIAGHGRILAAKEEGIKQVPCVFVEHLTEAQKRAYILADNRLAMNAGWD
                     DEMLAVELSDLQGEEFDLDLLGFETDELDKLLNGNDDAQDDDFDVDEELEKPAFSKTG
                     DLWLLGRHRLVCGDSTKPETYEKLMDGKLANLVVTDPPYGVSYQGKAGTIKNDNLKDE
                     AFYNFLYDAFSCMESVMANDASIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLYGWKKKGKHQWYTGRKESTIWEFDKPKKNGDHPTMKPIPLLAYP
                     ITNSSLSNCIVLDSFGGSGSTLIACEQTDRICHTIELDEKFCDVIVKRYIEQAGSDAD
                     VSVIRDGKTIAFNDLENPE"
     gene            complement(27905..28687)
                     /locus_tag="ACHAYA_000033"
     CDS             complement(27905..28687)
                     /locus_tag="ACHAYA_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HFM8603875.1"
                     /translation="MIEKVNPQHPDKVADRIAGAIVDLAYAEENNPKIAVEVLIGHGI
                     CHVIIETSTALNLNEISQAINRIAGKIKKDVVIVPQDKELADNQATEIRCGDNGIFRG
                     MPLTDEQLQLSKIARDLYQDHPFDGKYILDGERLIICQSHAKTNELKQKYPNATVNPL
                     GDWTGGPDVDTGATNRKLGSDMADGVTGGGIHGKDLSKADVSVNIYAFLKAQETGMPV
                     ELSCAIGDTEIDGVPYSEIVEVARDYVNELGGFEKLAEWGLF"
     gene            complement(28702..29274)
                     /locus_tag="ACHAYA_000034"
     CDS             complement(28702..29274)
                     /locus_tag="ACHAYA_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8603876.1"
                     /translation="MAKDGTQRGGRRPRSGERSQPLVDKVAAGKKASRMEFDLPDVEL
                     LIGEDIGEGVELEGNDYPEPSAYLSAKQKDGTVLGADEIYNSTFQWLKDRGCETLIAP
                     RMVESYAQNFARFVQCENALSQYGLLGKHPTTKAVMASPFVAMSQNFQKQANVTWYEI
                     QQIVKENASVEYTGDPNTDAMERLLRSKNG"
     gene            complement(29408..29773)
                     /locus_tag="ACHAYA_000035"
     CDS             complement(29408..29773)
                     /locus_tag="ACHAYA_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8603877.1"
                     /translation="MPMKPANPCKFPGCPNRTHDTYCDEHKTLARKRYEKYERDPAIN
                     KRYGRAWKKIRARYVAAHPLCEMCLKEGRFTSVEIVHHIKELSDGGTHDENNLMSVCK
                     SCHSKIHMTRRNTKDKTIM"
     gene            complement(29941..30369)
                     /locus_tag="ACHAYA_000036"
     CDS             complement(29941..30369)
                     /locus_tag="ACHAYA_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HFM8603878.1"
                     /translation="MTPKEYLKQAYRLNKRIDDRIKEKAELYQLAVTVSSTTFEEKIS
                     SGTRNTEAPFVRNLEKCYQLEAEIDAEIDKFVDLKRQMRGVIDELTNVDEKIVLINRY
                     LLEESWDKIAEDLHADVRTVFRWHSKALENLKLPDEPIVI"
     gene            complement(30366..31718)
                     /locus_tag="ACHAYA_000037"
     CDS             complement(30366..31718)
                     /locus_tag="ACHAYA_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFM8603879.1"
                     /translation="MKFVPHNYQKYAIDFIKDHKTAALLLDMGLGKTVTTLTAIKDLM
                     HDDFTIQRVLIIAPLRVTQSTWPTEIQKWDHLKDLSYSVVLGTPSQRMAALQNNANIY
                     LINRENLDWLINKSGFTFDFDMVVIDELSSFKNLKAKRFTSFMKVRHKVDRIVGLTGT
                     PSSNGLMDLFAEFKVLDMGERLGYYISRYRDKYFLPDKQNGMQIYSWKPREDAEQEIY
                     DKISDITISMKSVDFLDMPELVMNEVPVDMGLKEKQKYDEIKADLVLQLKEATIDAAN
                     TAVLSNKLLQMANGAIYDEFNVSHHIHDQKLDALEDLIEGTNGKPILIAYWFQHDLER
                     IKERFNVRQIKTSQDIIDWNEGSIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQSDTVVIHHIIAKDTIDEDVMLALKRKDKTQSCLIDAVKARLEGSK
                     "
     gene            complement(31718..32002)
                     /locus_tag="ACHAYA_000038"
     CDS             complement(31718..32002)
                     /locus_tag="ACHAYA_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFM8603880.1"
                     /translation="MREKQVEQALVKAVKRVGGICPKFTSPGLAGVPDRLVLMPNGKL
                     GFVEVKAPGKKPRSLQLFRMKQLTDLGFQCFVLDEIDQIPELLERIGGDV"
     gene            complement(31999..32208)
                     /locus_tag="ACHAYA_000039"
     CDS             complement(31999..32208)
                     /locus_tag="ACHAYA_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603881.1"
                     /translation="MKFKQWLQIGIGKRDLRFDEFYEFAMSDVTYPWKKSFDKQVAHL
                     MIHCAEKEYLEILRDAYFEYLTVWL"
     gene            complement(32346..34589)
                     /locus_tag="ACHAYA_000040"
     CDS             complement(32346..34589)
                     /locus_tag="ACHAYA_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFM8603882.1"
                     /translation="MLKMTLFTSNQVGNSKNGLFPTKQVVTDKETLVATVKLDHVMAE
                     YKGNYRKGDNFIQSDVIPLDCDNDHSDNPDDWVAPFEVAMQFPDVTFAVSYSRNNMKD
                     KGGKSARPRFHVYFPIPATTDKDEYVELKKQIQSEFPYFDNNAMDAARLLFGTPNSEV
                     EIYEGSKLITDFLEDDLFADWDNAQSEISEGSRNSSMSHIAGKLIKRYGATEDTYHKY
                     LEQSQKCNPPLPEEELKAIWNSAVNFGKKVKQQEGYIPPEQYNSDLSLEPTDYSDLGQ
                     ATVLAREYECKLHYSPSTGYLVYNGSYWEESKPKAQGVIHALTERQLEESETEIEKRT
                     KEMVSNGAFDVLASVGPKKAVTMFNTAQRHSFDLYQHAQEYKKFAVKRRDSKYLSSAL
                     TEAKPMLEIEQRLLDVNEFLLNTPTATFDLRTGKSQDHNSEDYITKQTECAPSDANQQ
                     IWLDALNTIFVGDQELIDYVQMIVGLAAIGKVYVEALIISYGEGRNGKSTFWNVISRV
                     LGNYSGSISADILTSQIRRNVKPELAEAKGKRLLIAAELEEGMRLNTSNIKQLCSTDE
                     IAAEKKYKDPFKYVPTHTLVLYTNHLPKVGAIDKGTWRRLIVIPFLATIDGNKDIKNY
                     ADYLFENAGGAILQWILDGAKKVIAADFHLTVPQVVANAISEYKTANDWLGHFLDECC
                     ETAVDFEEKSGELYAEYRAFCTRTGEYIRSSADFYTALEAGGFKRRRTNKGNKIVGLQ
                     LKSEFLE"
     gene            complement(34582..34926)
                     /locus_tag="ACHAYA_000041"
     CDS             complement(34582..34926)
                     /locus_tag="ACHAYA_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFM8603883.1"
                     /translation="MPRRKDYRTSVYVCSPFSGNVVVNHKNTKRYCRIALDRGYMPVA
                     AHLMYPQFMDDATERDVAMHMSLVLMGKCEEVWVVGDTISQGMAVEIEQAEYWKKHIR
                     YFDDEMKEVSNA"
     gene            complement(34919..35689)
                     /locus_tag="ACHAYA_000042"
     CDS             complement(34919..35689)
                     /locus_tag="ACHAYA_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFM8603884.1"
                     /translation="MNELKVFENAEFGSVRTVMIGDVPHFVGKDVASILGYTNPQKAL
                     RDHVDEEDKTVNESFSVNGTMAILINESGLYSLIISSKMPNAKKFKRWVTNEVLPSIR
                     QHGVYATEDFITKSIEDPAWAISVLKQLQEKKEMVAMQQQMILEMKPKVSYYDLILQN
                     NSVMSISKISKDYGMSPQAMNKLLHELGIQYKQGKMWLLYQKYANQGYTQSKTHAIDA
                     ERSVMHTYWTQKGRLFLYDLLKSKKNILPVIERGDNNA"
     gene            complement(35900..37837)
                     /locus_tag="ACHAYA_000043"
     CDS             complement(35900..37837)
                     /locus_tag="ACHAYA_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFM8603885.1"
                     /translation="MKHLSLDLETYSSVDLSKCGVYKYSESPDFEILLFAYSVDGGPV
                     EVVDLKMDETIPAEILTSLSDETVIKHAFNASFERVCLSRFLGLDGFLNPRGWQCTMV
                     WGATLGLPLSLVGVGAVLGLDKQKLTEGKDLIRYFCVPCNPTKVNGGRTRNLPHHAPT
                     KWSAFKTYNSRDVEVEIAIQGKLSNFPVLDSVWKEYWLDQEINDRGIEIDKDFVEAAV
                     QLDELSKAGLNQQLQELTGLENPNSVLQMRGWLLDHGLETESLGKKAVAELLEQPIDE
                     NLKQVLVLRQQLAKSSVKKYQAMQNFACSDNRSRGLFQFYGANRTGRFAGRGIQLQNL
                     PQNHMSDLSEARSLVKQSNFEMLNILYGNVPNVLSELLRTAFVPREGMKFIVSDFSAI
                     EARVIAWLSGEKWRQEVFKNGGDIYCASASQMFGVPVEKHGVNGHLRQKGKIAELALG
                     YGGSVGALKAMDALEMGLTEDELQPLVNSWRDSNINITELWWAVDKAVKTCVKERRST
                     STHGIKFEYKSGFLFIQLLSGRKLVYVKPKIGENKFGGEAVTYEGVGATKKWERLESY
                     GPKFVENIVQAIARDILCFAMENLRKYRIVGHVHDEVIIEVPMESKVEDICQLMSQTP
                     KWAEGLLLNADGYECEFYQKD"
     gene            complement(37898..38440)
                     /locus_tag="ACHAYA_000044"
     CDS             complement(37898..38440)
                     /locus_tag="ACHAYA_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFM8603886.1"
                     /translation="MSKTSKVITGTNTRLSYFHGWEPASINGGAEKYSTAVLIPKSDK
                     RTIDTIEKAIDAAIEEGLAKFGGKKPNKAEIKLPLRDGDIERDDEAYQGHYFINANST
                     TAPQIVDQHVQPILDRSQVYSGCYARVSINFYAFNANGNRGVAAGLGNIQFVRDGEPL
                     GSQVSASDEFETLADDDFLA"
     gene            complement(38442..39584)
                     /locus_tag="ACHAYA_000045"
     CDS             complement(38442..39584)
                     /locus_tag="ACHAYA_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFM8603887.1"
                     /translation="MPTQHAVLSASSSNRWIHCPPSVRLSEHFENKSSPYAQQGTDAH
                     TLCEYKLNKFIGNDVTDPTSTLEFYDEEMEQCAESYATFVMEEVAKAKQTTVDSIVIV
                     EQRLDFSRYVPDGFGTGDCLVIADGTLSVIDMKYGLGILVDAYQNPQMMCYALGALEL
                     FDVIYDIQEVKMTIFQPRRENVSTYTLLKEELLQWAKDVLSPAADLAFKGEGEYQCGK
                     WCGFCPAKNSCRARADHNLKLAQYEFKPPELLSDDEIEEIIGKVDDLVSWSNDIKDCA
                     LKLALGGKQWTHHKLVEGRSTRKYSNDNDVAAAVIKAGYDPYDKKLLGVTAMTKALGK
                     EKFNEILGEYIIKPKGKLTLVDGSDKRQAVTVNNLNEEFKPLTEEQ"
     gene            complement(39585..39899)
                     /locus_tag="ACHAYA_000046"
     CDS             complement(39585..39899)
                     /locus_tag="ACHAYA_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HFM8603888.1"
                     /translation="MSKTKLFLQVVEDVQALADSLQELAVAMKTNEPEPQVETPEPTM
                     ETKSEVKQPTLEEVRGLLARKSQEGKSTEVKALIEKYGASRLSDVPTENYAALVADAE
                     GL"
     gene            complement(39904..40110)
                     /locus_tag="ACHAYA_000047"
     CDS             complement(39904..40110)
                     /locus_tag="ACHAYA_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603889.1"
                     /translation="MNLKHKIRVNVTDDTGNKETVLQGGVRHLPRWFARWLFGKGNMD
                     KILVLTPGDTIESVEIREVNKKGA"
     gene            complement(40207..40824)
                     /locus_tag="ACHAYA_000048"
     CDS             complement(40207..40824)
                     /locus_tag="ACHAYA_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603890.1"
                     /translation="MSRFMKTHQNDRSTYKYVFERTDANGRIQKEVVEIRPGEDGVTE
                     LNIQELHRADDRAIYNFYKNARPERTEEEKEKIKAWKEKFREDFNNEHGYYPMDEYVD
                     GEANDYFKRNYNLSLNEFDDECDEGDNPIQSLVYDAYQDNEEDPRIDRLREVVTQLLP
                     YHQEIYHMLVTENMSQTEVADKLDKKKQSINRTFGIIKDKIKELF"
     gene            41219..42169
                     /locus_tag="ACHAYA_000049"
     CDS             41219..42169
                     /locus_tag="ACHAYA_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HFM8603891.1"
                     /translation="MEISEITQTLYPFMGSEYVKPQDFLIALLDSVSNDDEATIFGFS
                     DDNLRRFYNGTRPLTQEYAVRVAGKFNIDKCTTFIYERLIDDAPTVLKQQLIDKGVVI
                     PKDTDVSEIVAHLLLDEITSIASQTKVKTKKAKPKLSELELASLRINTNGDFSYNDEV
                     KKFFDDLPVPLDIRAEEMTYVIALFSAYADADGYEVYEHTNDLPNSRLKDFKRQRRNY
                     YKAESIRRGVRDNFTQEEGTEHFEALKEDMYDGIEEVYEEDYDDGVERLKAVLQQSSV
                     ITLNGSILSFIPGLLQNSAKKGICHMLVNDGKIKWVTEDE"
     gene            42162..42608
                     /locus_tag="ACHAYA_000050"
     CDS             42162..42608
                     /locus_tag="ACHAYA_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 2"
                     /protein_id="HFM8603892.1"
                     /translation="MNSLFNTPFEISVRVMLLLSEFKEPVSADRILITDFITTYGRDF
                     EISDYNLNGDNSYRFSEFIARRELVKDAVKNLVLQHVIQPSQSKEGFKYALSEDGLKL
                     ISQFVSVYAEKYLQIADESVKYIQDKSDVELLRQINQRAKEFKGEQ"
     gene            42611..44443
                     /locus_tag="ACHAYA_000051"
     CDS             42611..44443
                     /locus_tag="ACHAYA_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8603893.1"
                     /translation="MQNYYIERVTIKGKDREDATVKFTNGLNIISGPSNTGKTSIVTA
                     IDFLYGAKKDAAPFSTDATGYSKVELIIQSTNGKYSLTREFDSNSILVSKITSSGEYV
                     DYEKYNARSGKNPISHFWLQLLGIDDDYQIIKNEDFKRQKLTWRTLAPSTLIPQDRVN
                     TSASVLFPGETTQRTAFLSSLLLLLYGSDFANSEENETKEHKKIRRNSVINYINSSLS
                     ELYENYEVAKSELPEAPSDDFQQIVDGMFAELETIEKQINDALASSKILIKEIYQKEQ
                     YLHETQLLLNRYKQLHYQYQSDIERLTLIVDGERLLNDHNEHDTSHSCPFCNGELSKP
                     VQTTYIDTARSELHSLLSQLSDLTEESHDTEVELQELMEQLDELKTRKKSLDAAINDE
                     LKPKAETLKDRINKIETYRDIKQQLISMEKMQNKLKSDLEKIENEPEDERILYKPKEK
                     FDAEFYKEMSELLDSMLRNMHYKPNEFRNAFFAKDIFDIKINGNLKWTENGHGYTAFI
                     NSVVVMAYRHLLYNHAKYTPSVFIIDSPLLGLDERKKQDGDDNMKEGLFKYFMDHQNE
                     GQLIIVENTTNLPALNYDDKQVNQIVFTEDKNNGRYGYLYDIYN"
     gene            44459..44662
                     /locus_tag="ACHAYA_000052"
     CDS             44459..44662
                     /locus_tag="ACHAYA_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8603894.1"
                     /translation="MKFSYNPLWKLLIDRGLNKAQLRKMTDISSATLSKLNKGENVTT
                     DILLRICEALDCDLSDIVEIVRD"
     gene            complement(44713..45849)
                     /locus_tag="ACHAYA_000053"
     CDS             complement(44713..45849)
                     /locus_tag="ACHAYA_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8603895.1"
                     /translation="MANSLDSIFEDDAFDDLTKQVEKKKVVKQDPEVAKFQEIIDFVN
                     ENGREPEKTPEWSSERALWARLSGFRGKPERSEKVKKYDTLGLLEKEFEPDGEFLADS
                     VEIDDELNLDDILGSDDMIDDFSDLLDVSRYRRTVNAADKIGRRKHASHFEKYKSLFD
                     QVHADIASGRRKIIPFEQYDIHENRFYIQNGVMMYIVSISKDTFIADNGKTNARMHVV
                     YENGTENKNLLLQSLASSLYSTERHGRMVTEPVDEETLAESFGEEFTTGYIYVLKSLS
                     TNPDISKWNNLYKIGFTRNDVESRIANAENDVTYLNAPVKVMLSAEVQNVNAQLLERT
                     LHHVFQDKQVVFQDGNFKKATEWYMVPLNDIESKINEVIAGLQK"
     gene            complement(45839..47794)
                     /locus_tag="ACHAYA_000054"
     CDS             complement(45839..47794)
                     /locus_tag="ACHAYA_000054"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8603896.1"
                     /translation="MTSNLFEINFDKNQSKTTNDLGMREMQERVYEKRASQYLLVKSP
                     PASGKSRALMFVGLDKLHNQGIKKVVIAVPERSIGKSFKDTELSKFGFYWDWEVKPSD
                     NLTIGGGEKSKVQHFVKFMNSTDPSDNILIATHATLRFAFEELDDSLFDNTLLAIDEF
                     HHVSRDDKSVLGNALRSIMSNSNAHILAMTGSYFRGDSVQILEPADEDKFDKVTYTYY
                     EQLEGYKYLKSFAMGYSFYRGKYTDALDKVIDVNKKSIIHIPNVNSGESTKDKYAEVD
                     EILDLLTDGGNITQNEDGIYEIERPDGKKLLVADLVNEEGREKVSAYLASITDDIKDL
                     DKLDIIIALGMAKEGFDWPFAEYALTIGYRNSLTEVIQIIGRVTRDSPNKTHAQFTNL
                     IAQPDAQDDEVLFAVNSIMKAISASLLMEQVLAPVYKFKPKDGKPDKPGDLSIRGLKP
                     ADTTERTKKIIENDMEDLKSSILQSQDIQNAIVSGADAKMINKTLIPRVIIDRYPDLT
                     NEELETVRQHTVADINIAAGRTTTSEDGSREIIKMADRFINIDELNIDLIDQVNPFQR
                     AYEVISRDIDAPTLRFIQDYLSSNKYDFTDEQLVAAYQAAKKFRAENGRNPDRNSKDD
                     GEKYLAFALLRLAEIKREREAAKEDGE"
     gene            complement(47787..50504)
                     /locus_tag="ACHAYA_000055"
     CDS             complement(47787..50504)
                     /locus_tag="ACHAYA_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFM8603897.1"
                     /translation="MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITR
                     AKSKFDNGEPFVIKNKLHYEEIEGNVVIAIDAIAQSIKEQKSKPRYILVNDYLDIAAI
                     DTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDN
                     PDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRTAEDGSNFNEVIKELFAIL
                     DINELARNGKDEWLLEFPYVNGKLFGEPHVDLTFSKVSRELLIEAGELLNWNEINPDI
                     LGAMIQSVASAEDRHVAGMHYTSVPNIMKVIKPLFLDDLTESFETLKLRSEENELKDI
                     TEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLG
                     DLQNNDSMPLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELL
                     PLKDAGNIVCANSLRIDWNEVLRLKENHEIYIMGNPPYLGKSLQNAEQREDMEKLVGD
                     IRKYKLLDYIFGWFWKAKQYIATSHGSFAFVTTNSICQGEQVSILWPYLLKDEVRISF
                     AYQSFKWNNNAKNNAGVTVVIIGMTNNVNIHPKLYLENGSVMSVSNINAYLANGENVF
                     VNESRNSISNLPSLKFGSMANDGGNLVFSVAEYWNLIQKYPDIKHLFKKYLGSTGLIN
                     GDLRYTLWLTEEDATRYKQNSLIKETIKNVKSYREKSKRKETRALAEKPWSFGEVRYD
                     ETSENSILIPRVSSEKRDYVPMGIVGSDTIISDSAMAIYNAPMWLLGLLESRMHMVWL
                     RSVGGKLETRYRYSAGLVYNTFPIQDLSAQRKNEMTRVMTEILDLREYEGGTLADLYN
                     KDSMPESLRKKHQELDGIVDRAYQQRPFESDEDRLGTLLKLYQEMTIND"
     gene            complement(50505..50726)
                     /locus_tag="ACHAYA_000056"
     CDS             complement(50505..50726)
                     /locus_tag="ACHAYA_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8603898.1"
                     /translation="MSISYNKLWKLLIDLNMNKTQLREISGVSPNVIAKLGKNELVAM
                     ESLLKIAAALNVDVSEIISSDVIDAEDVK"
     gene            complement(50875..51711)
                     /locus_tag="ACHAYA_000057"
     CDS             complement(50875..51711)
                     /locus_tag="ACHAYA_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8603899.1"
                     /translation="MKQIFIFSDTQKDNISYFEREPAILVSMSRSDLPYLKEQDFSNY
                     PAVYVLIGGNKRYVGQAAGQSISLRLSQHFLKEYKAWVESVLFFTRADGKMSKADTDY
                     LEKRLIQDFQEKSDYEMMNSTAGNTSYIDKLQKAKSDQLYDTVFEIIDEIANIDLFGT
                     SEDSETQIYEFSSSGQYEIEYDGKKISSKSARGLLVDFVSQLFADGNYNNQLQELIVD
                     ETPSFAIILGRKPSMYNGRPNSARVADGVWVYANFNKKNVRIKIDKLARQLGIPVKIK
                     WN"
     gene            complement(51920..53296)
                     /gene="rlmD"
                     /locus_tag="ACHAYA_000058"
     CDS             complement(51920..53296)
                     /gene="rlmD"
                     /locus_tag="ACHAYA_000058"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8603900.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIECV
                     GLLTRVER"
     gene            complement(53407..54462)
                     /locus_tag="ACHAYA_000059"
     CDS             complement(53407..54462)
                     /locus_tag="ACHAYA_000059"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8603901.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(54472..55902)
                     /gene="gatB"
                     /locus_tag="ACHAYA_000060"
     CDS             complement(54472..55902)
                     /gene="gatB"
                     /locus_tag="ACHAYA_000060"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM8603902.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(55899..57371)
                     /gene="gatA"
                     /locus_tag="ACHAYA_000061"
     CDS             complement(55899..57371)
                     /gene="gatA"
                     /locus_tag="ACHAYA_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM8603903.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(57371..57676)
                     /gene="gatC"
                     /locus_tag="ACHAYA_000062"
     CDS             complement(57371..57676)
                     /gene="gatC"
                     /locus_tag="ACHAYA_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM8603904.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(57782..59818)
                     /gene="ligA"
                     /locus_tag="ACHAYA_000063"
     CDS             complement(57782..59818)
                     /gene="ligA"
                     /locus_tag="ACHAYA_000063"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM8603905.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(59835..62069)
                     /gene="pcrA"
                     /locus_tag="ACHAYA_000064"
     CDS             complement(59835..62069)
                     /gene="pcrA"
                     /locus_tag="ACHAYA_000064"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM8603906.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            62224..63783
                     /locus_tag="ACHAYA_000065"
     CDS             62224..63783
                     /locus_tag="ACHAYA_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM8603907.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(63894..65825)
                     /locus_tag="ACHAYA_000066"
     CDS             complement(63894..65825)
                     /locus_tag="ACHAYA_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8603908.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(65843..66757)
                     /gene="pfkB"
                     /locus_tag="ACHAYA_000067"
     CDS             complement(65843..66757)
                     /gene="pfkB"
                     /locus_tag="ACHAYA_000067"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8603909.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(66754..67506)
                     /locus_tag="ACHAYA_000068"
     CDS             complement(66754..67506)
                     /locus_tag="ACHAYA_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8603910.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(67665..67874)
                     /locus_tag="ACHAYA_000069"
     CDS             complement(67665..67874)
                     /locus_tag="ACHAYA_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603911.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(67885..69213)
                     /locus_tag="ACHAYA_000070"
     CDS             complement(67885..69213)
                     /locus_tag="ACHAYA_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603912.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            69349..69504
                     /locus_tag="ACHAYA_000071"
     CDS             69349..69504
                     /locus_tag="ACHAYA_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM8603913.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(69578..70165)
                     /gene="yihA"
                     /locus_tag="ACHAYA_000072"
     CDS             complement(69578..70165)
                     /gene="yihA"
                     /locus_tag="ACHAYA_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM8603914.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(70248..71498)
                     /gene="clpX"
                     /locus_tag="ACHAYA_000073"
     CDS             complement(70248..71498)
                     /gene="clpX"
                     /locus_tag="ACHAYA_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM8603915.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(71745..73034)
                     /gene="tig"
                     /locus_tag="ACHAYA_000074"
     CDS             complement(71745..73034)
                     /gene="tig"
                     /locus_tag="ACHAYA_000074"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM8603916.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(73188..74120)
                     /locus_tag="ACHAYA_000075"
     CDS             complement(73188..74120)
                     /locus_tag="ACHAYA_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603917.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(74277..74936)
                     /locus_tag="ACHAYA_000076"
     CDS             complement(74277..74936)
                     /locus_tag="ACHAYA_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8603918.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(74939..75964)
                     /gene="sppA"
                     /locus_tag="ACHAYA_000077"
     CDS             complement(74939..75964)
                     /gene="sppA"
                     /locus_tag="ACHAYA_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8603919.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(75977..76267)
                     /locus_tag="ACHAYA_000078"
     CDS             complement(75977..76267)
                     /locus_tag="ACHAYA_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603920.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            76677..78482
                     /gene="glmS"
                     /locus_tag="ACHAYA_000079"
     CDS             76677..78482
                     /gene="glmS"
                     /locus_tag="ACHAYA_000079"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM8603921.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(78554..79909)
                     /gene="glmM"
                     /locus_tag="ACHAYA_000080"
     CDS             complement(78554..79909)
                     /gene="glmM"
                     /locus_tag="ACHAYA_000080"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM8603922.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(79933..81102)
                     /locus_tag="ACHAYA_000081"
     CDS             complement(79933..81102)
                     /locus_tag="ACHAYA_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM8603923.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(81099..81986)
                     /gene="cdaA"
                     /locus_tag="ACHAYA_000082"
     CDS             complement(81099..81986)
                     /gene="cdaA"
                     /locus_tag="ACHAYA_000082"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM8603924.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(82175..85855)
                     /gene="nifJ"
                     /locus_tag="ACHAYA_000083"
     CDS             complement(82175..85855)
                     /gene="nifJ"
                     /locus_tag="ACHAYA_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM8603925.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            86053..86574
                     /locus_tag="ACHAYA_000084"
     CDS             86053..86574
                     /locus_tag="ACHAYA_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM8603926.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(87273..87838)
                     /locus_tag="ACHAYA_000085"
                     /pseudo
     CDS             complement(87273..87838)
                     /locus_tag="ACHAYA_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(88017..88673)
                     /locus_tag="ACHAYA_000086"
     CDS             complement(88017..88673)
                     /locus_tag="ACHAYA_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603927.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(89241..90176)
                     /gene="whiA"
                     /locus_tag="ACHAYA_000087"
     CDS             complement(89241..90176)
                     /gene="whiA"
                     /locus_tag="ACHAYA_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM8603928.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(90210..91208)
                     /gene="yvcK"
                     /locus_tag="ACHAYA_000088"
     CDS             complement(90210..91208)
                     /gene="yvcK"
                     /locus_tag="ACHAYA_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM8603929.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(91205..92089)
                     /gene="rapZ"
                     /locus_tag="ACHAYA_000089"
     CDS             complement(91205..92089)
                     /gene="rapZ"
                     /locus_tag="ACHAYA_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM8603930.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(92224..92955)
                     /locus_tag="ACHAYA_000090"
     CDS             complement(92224..92955)
                     /locus_tag="ACHAYA_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM8603931.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(92959..94224)
                     /locus_tag="ACHAYA_000091"
     CDS             complement(92959..94224)
                     /locus_tag="ACHAYA_000091"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM8603932.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(94487..97303)
                     /gene="uvrA"
                     /locus_tag="ACHAYA_000092"
     CDS             complement(94487..97303)
                     /gene="uvrA"
                     /locus_tag="ACHAYA_000092"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM8603933.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(97313..99307)
                     /gene="uvrB"
                     /locus_tag="ACHAYA_000093"
     CDS             complement(97313..99307)
                     /gene="uvrB"
                     /locus_tag="ACHAYA_000093"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM8603934.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            99566..101068
                     /locus_tag="ACHAYA_000094"
     CDS             99566..101068
                     /locus_tag="ACHAYA_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8603935.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            101070..101807
                     /locus_tag="ACHAYA_000095"
     CDS             101070..101807
                     /locus_tag="ACHAYA_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8603936.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
BASE COUNT        28233 a        22716 c        18132 g        32984 t
ORIGIN      
        1 gttatcataa atcttaggat atttggaaac accacatcga agaataaatg acagaaaatc
       61 tctctcctca gcaaaactag agcaatctat ttttttcaat gccgtgtaat ctgtatcttg
      121 aaaaagctta tctcctaatt cattgccata ctcttttcca atatacattt tattggaagg
      181 ttgaacgtca ccagcaacgt ttggaagatt aaatggaact ttagacagct cacttccagc
      241 tgatagtttt tcattgtagt tatcataaaa ccaattaatg aaactgatag atttttcaac
      301 agtgtcaatt tgtcgattaa tcgttccaat gatcaaatct gaactgcttt gctcagtgaa
      361 ttcaattgca agatattttt cactgaatgc atctagaata cgtttatctc ctgtacgttc
      421 agctttgtat gcattagctg tttcttccca tttatttttc caacttgatg aagtcttaat
      481 ctgataaatt aactcatcaa catatgtttg tttcaaaatg cttaaactaa cccaagaaga
      541 cagctctttt ggaagcacag taacacctgt atcggtcggt aagaatacct tatctgattt
      601 catcaaccaa ttgtcactat tgtcctttaa tagcttaggg acaatcggag aacttttata
      661 ttgctcactc caccataaga acacttttgt ccttattcta ttttcccaag aagatgaaag
      721 aatgttgatt ttatcagata gttcatctac aacaaacgac aggccagtgt attcattata
      781 attagcaagt tcttgtacta gttggtcatt ttcgtttgtt tctaataatt gcaacaaggt
      841 ggaaaatgtc tcacctatta gctcctcagg gaattcttga aggaaaagtt ttggattatc
      901 tttgatagaa atatattgcc aattgacagt cggtagtatt tgggcattag ataaaatacc
      961 caagaattcg ttcattaagt caaatacatc taagtcccat agtttattct ctttaaaagg
     1021 agttaccatt tttaatgcta tgtcttcata ttcaggctta gataattttt ctgctacttc
     1081 agaaataaat ttgagcacct caataaatat tttcttattt atttgttcac tatcatccgg
     1141 gacgtcatca cgtgaattat ttgttataaa cggtgcattt ataagtgcat gaattggtgt
     1201 accaaaatta cgtactggaa agtagcaata tagtttttct ttggagtaat cgtaatcaga
     1261 atctttagaa acggctagtg caattttagc aactttatct cctatcatag attctttgtc
     1321 aaaaacaaaa tattcttcag acggtgattg accagtaaca tcactttttt ctatcaatac
     1381 atcatttcca tccacaattt tctctgatat tttttcatca tcagttgttt caataacaat
     1441 ttttgttata tttggtaaaa ataacagtga cttataaaat ggctgctgaa gttgtttaga
     1501 aacactcgtg cctttactta aattagtttc ttttagtttt actcgaatta atgtatcaaa
     1561 accagaatca attttgttta ttggttgagg acagttcatt attggaaaac ataaatcata
     1621 atcttctttt ttccaatttt tcttctgctc aaaaattaat tcactgaatt tgtcagaatc
     1681 tcttgataca tactgctcaa aaagcttttt tgtgaattct tcactaaatc gaatagcaaa
     1741 ttctcccgaa tacagttcga cccactcagc atcgtttagc aaggaacgaa aaccagtccc
     1801 cttatttccg atattttgtg atgatttatt actcgctctt cctaagcaca atctttcaat
     1861 tgtatctttt gaaaatgaag tgcctgtatt cccaacttcc aagatattat ttttatatgt
     1921 tatctgaact ttcacttcgt cattgctagc atcattttct gattttatat ctccataggc
     1981 atcgtctaca ttctgtaata actctaaaat ctgacgccca ttataatcac gcatcaattc
     2041 atattcttcg ctgcaggcct tttcaagttc attactcaac tcagaatccg tatatttacg
     2101 aataaaaata tcccttaatt catcaatggt attccattgg ctcacagaac ttcgccccca
     2161 gcctattttt tacataatat attatatcat caatcacttt atgaaatcca atcaataaag
     2221 acgttaatat ggctacccac attaacgtct agtcctatct attcaaatta aatttctaca
     2281 gtcactctca ccccactttt gaaatcaaca acaatgttct tttcaaaaat ggtgaccttc
     2341 tcaatcaacc ttcgaacaag cacttcatta tactcattga tgacatcgtg ttcttttaca
     2401 aagactacca tctcatcaac ccgttgttgc aaattagtat tcgctgctat ttgttttgca
     2461 accgcatctc gttcatctct tagtgaaaat atttggcttg ccaattcatc gtaaccctga
     2521 ccgccaactg ccaggctaat aatttcagct tcaagctctt tcaactgact gtctagttct
     2581 gccaatcggt cagttaatcc atcagtcata actgagttga tattgtcatt taattgctga
     2641 agaaaagcat cacgatcgat taacgtttca ttgattgcct tgataacagc tccatgcagg
     2701 tctgtttcgt agattgttcg acccgtgcaa ttatgttctc gtttatccac tctggatttg
     2761 catcgccaca cagttcctcg attcttccaa tgaactctgc ggtaagcatt gccacagtca
     2821 ccgcaaactg tgataccaga taacgcatac ttcccagaaa agcactgtga ctttttgttt
     2881 ttcattgatt tacgccggtc tagttcttgc tgggcttgca tgaagatatc ttttgggata
     2941 atggccggat ggctatcttc aacataatac tgctgagcgt aactcccgtc attcttcact
     3001 cgtttctttg tcaagaaatc ggtagtatat gttttttgaa gcagcgcatc ccccatgtac
     3061 ttctcgtttc gaagaatcaa tctgactcct tctcctcgcc agagctttcg cttccctccc
     3121 gttaggatgc catcagccat tagaccatta gcaatttctt gcaaggaact gccctcaagg
     3181 tattcgcgga agatgcgttt gatgacttct gcttcctttg gttcaatcac caagttacca
     3241 tcttcgtcct tagtgtaccc aagaaaatga ttgtggttga tacgtagctg tccctgctgg
     3301 aaacgatact gtacgcccat cttggtgttt tgacttagtg actggctttc ttgctgggcc
     3361 agacttgcca taatggtcaa aagtacctca cccttcgcat ccattgtatt gatgttttcc
     3421 ttttcaaaaa acactgcaat cttcagttct tttaactgtc gtatgtattg caagcaatca
     3481 accgtattac gggcaaagcg tgaaattgat tttgtgatga tgtactcaac ttcgccgttt
     3541 tgacacgcct caatcatccg gttaaaaccg tcacgctttt tcgtcccagt tcctgaaatc
     3601 ccttcatccg caaatattcc tacaagctcc caatcgggat ttctttgaat gtagtccgta
     3661 tagtgttgaa cctgtgcttc gtaagaactc gcctgttcct ccatttcagt agaaacacga
     3721 caataggctg ccactttcaa ctttggtttc tcttcggtat tctctgctgc ccattgtcta
     3781 ggcttcattg gaataaccgt cacctgtttt cctgccattt cacttaacct ccttgatttt
     3841 tgaatatgca tattgcgctt gtttgaatgg gtcagaatac ttttcggtaa tcttgccaga
     3901 tttaaaatga gtgggtatta ccttcgtatc tggcactttg atctccttgc gttttttaaa
     3961 gaacttaatt cttcgttctt gctccagatc cgcttggaag aaaatatcct catcaatgat
     4021 ttttggatat atctcatttc ctaagtaaac ctcattcatc aaaatacgtt tcataaaggg
     4081 atgcggcttg ttaatgccag agcgtttgcc agcttcttgt aagccaacac cctcaaggta
     4141 aatattgaag aactcacgta tttgttgagc ttgctgttca tccacgttga tttctccatt
     4201 cttaatgaca tacccgtatg gaattgtatt gttcattctg gtaacctctc tttcaaaagt
     4261 agtccgcact taagttcaaa tccgacttct tggcggttgt aaactgtaat gctgtttgtg
     4321 aacttcttga ataaatcttc atcccagtcc gtcaacatct ctgccatccg cgcgaatttg
     4381 tataaggctt ctagttcaat ttgctgagtg cttttgccgt tgactgactc aatgatgagg
     4441 cgctgctttt cttgtgcgac agccctctct tgttgaagtt tgctttgttc ttctaaagac
     4501 accttcgttg ataaaatact ggaagcagtc aaacttgcca gaacctcaag cttttcttga
     4561 atcttgtcga tttcatcagc aagcacttcg agcccttcct gattcgcacc tgctttattc
     4621 aaagacttca taaaaggttc cagaaccaac ttgtgtccga aaatcaactt attcaacatg
     4681 gtcaaataag caaccttcaa atcttcatcc gtaattggta atgcatcgca gctatcttta
     4741 tttttaagat gccccgtaca cttccactga accttcttcg tattaccttt ccaataagtg
     4801 tgaacccgct tgaatttgct gccgcattta tcacagatga tgttgcctga aaatgcgtat
     4861 cgcttggtgt actttttccc gtcaccagta ttccctttgg cctcagcttt tctccgaatg
     4921 agttcttgaa ctcggtcgta atcttcttta gatactatgg gttcgtgatt gtttttgatc
     4981 tggtacattg gcaattcgcc cttgtttatg cgtcgtttgt actggtcatc aacaaaggtt
     5041 tgttgcatcg tcaaagtccc ggtataaact tcatttttca aaatacttct gactgatgtt
     5101 gcttgccaat ttgccccacg tttagtgaat tgttttccat cgtttaaccg cttggcaatc
     5161 attccagtcg aaatgccatc taatgcccac tggaacattt gtttaacaac ttttgcttca
     5221 gctgcaacga tgtgaaaatc atagcctttc cgcttgtagc catacggcgg gtaagctttg
     5281 aatttccctt tagacatccg gtttcgaatc gcccacttca agttttctga atgggaatag
     5341 gattcacttt ctgccaagct tgataaaatt gaaagcaaca actcgctttc catatcacca
     5401 gtattaatgt tttccttttc aaaaaagatg gtgacaccaa tatcagttag ctgccgaacc
     5461 agattcaagc agtcaactgt atttcttgaa aatctggaaa tagactttgt cagaatcaaa
     5521 tcaatctttc caagctctgc atccttaatc aatcgtttta aaccatcacg ttttacagtc
     5581 ttggttccag aaatcccttc atcataataa acaccgacat agtcccattg ttcatggttc
     5641 ttgatgtgat tttcatagtg agtaatctgg gtttgtaaac ttgccagctg ttcattactt
     5701 gatgttgata cacgagcgta tgctgccacc tttagtttgg aagcttccgg caactctgga
     5761 cgtgccaatt tatgtatcgt tttcatcagc tcaactcctt tccattaggt gacatgttag
     5821 ctctaaagtg ccgttatatc aacgtttcag accattatcc ttgataaaaa aggattgaat
     5881 gtttcccggt ttttattgtc gattttgacg tattcttcca ggttgatttt accggtaaca
     5941 agtaagttca gaaggatctg ctgtgaaatg taataggctg cttcatctga atagttaatt
     6001 tctgttttct tgttagtcat tgctttcgcc tcctcaagat acaggcgaag aaaatccatg
     6061 aaagtaaacc caatcacaaa ttttcctttg cctttcatcc atatgcgaaa gaaataaccg
     6121 actcgaaccc acaaaacgaa aaaagcctgc aagaatgaaa cgaatcactc ttgcaggctt
     6181 ctaagtttgt ttatatatag taggaattat tctttgtaaa ttttgacttt ttacttccca
     6241 aacatcaaat aagcctttac aagcttcatc caataaataa caatataaat atcaattgtg
     6301 ggaaggagaa agttatgagt agttttgata tggaagggtt agaaaaagaa tatgaacatt
     6361 tttgcaaatc tgcgacaaca aaggaactgg aggaatttga tgaaagcttc aaacaagaat
     6421 ttgaaaagtg ctttaaaacc atagaggctg aagaagcttt ataccagttt ttacaagcca
     6481 atataaacgc gatgaataat cgtatggaat caatagaatc gcctcttttc tgcatcaatt
     6541 acaaagagca ggtatttcag ttaagaaatc atttaatcct aatgcaaaat gctgtaaaca
     6601 cctaccttaa agaaaactag acgaaaaaaa ggccccacaa taaaacttgt gaggctgttt
     6661 tgaattataa agacgtcaaa ctacttattg aaaattttgt agctcgttgg aaccatcgtg
     6721 aagttttctt cagtaattgg gccatcataa attttgattt tgcaaacttt tgacgccaca
     6781 tcaaggtcgt gaacaaagta aattttatat ggtgtatctc caagggaggc agcaacctca
     6841 ttagtactca tttcaaatcc gtcagagaag tttgtttttg aagctttcac ttcaatatgg
     6901 atttcattac ctgattggtc aaacgcacaa atatcatagc catgaccatc tccttcagtt
     6961 ttagaaacat gttcaggaag ttttgttttg ttcttttctg cttcctgaat gaggaaatca
     7021 agtacgattt cttcgcctag agcacccgtt ttttgttttg ccttgttgac acgctcccaa
     7081 tcagtatggc gtccagtgaa ttttcgtttc tgagtcccat tcgtgcgatt tggtcccact
     7141 tcatcaacaa gaacagtaaa tccttttgaa ctttcttgct caatttcttc aacttcctcg
     7201 tttgtaagat ccagttcaga cggcaacgat tgtgtatcat cgattgggat aagatgacga
     7261 ttttcaatca atgcaacaaa atcggcatag gtaatttcat tcattccata ctgatcccag
     7321 aagtaggccc atgaattcgg ctttttacgc ttctttttgc taggtgtatt ccaatcgaag
     7381 ttttcaataa atgaatcacc ttgtttaatt gccggttcga ttgtaaatgc gtgtgtaatt
     7441 actgccgtaa catttccagc ttcatcaggt tcagaaatgc tctcaacgta tccgcccccg
     7501 taaatttccc acttacggtc tttagtaatt ttccccggat gacgattcaa aacaaccgcg
     7561 ccggctttca atttaccata ttggcgcatg ttccaaccgt aacttttagc atcatcagcg
     7621 tatttcaact gaccatcatc ttctgttttc ccgtgatcta aaatgaaagc ttgttggctt
     7681 tctgttggtt ctggtaacaa atctttaaac gtaaccatat cgatacctct ttctataatt
     7741 caatcggaac aaatttatgt tctggttgaa ttaataataa cacgttcgat attttaagtc
     7801 aacaatccgg acataatttt gttccgatta aatttatgaa atcaaaatat aatcgtcaga
     7861 ttccgtctta ccagtattct taccgacagc acaatatcca atcgggcaac gagcccatac
     7921 ccaaccatcc gcaatcgtca tcttctctga cttcggaagc gtccaagttg cgcctttatt
     7981 gtatgaacca acaaccgcac cacttaaact tggcttgttg cgaacgttga gaccactcac
     8041 ttgaaccaca agagttttca cgttggatgt tgatgaccca gatggataaa cttgattacc
     8101 gttctggtcg aacaccttat aaccagaatg gctatctgca gcccgcttcg cattgttcaa
     8161 gtccttatag gcaccaattt gtgacttggc gtcagcccat gatttgcgaa cacgatacag
     8221 accactagct ggcgtgcttg gggttgatga accattatta atcgcattgg ctttatccgc
     8281 aatctcctgc agtttaccaa gcaactgtcc cggacatgat gtggccgaaa aatgtgagtg
     8341 agggaagata ttcttgttca ccaccaattt ccctagacca taacgtttgg caatatctgc
     8401 acacaacttt gcacttgcat caatagttgc ttgattgata ccccagttcg gtgatccagt
     8461 gctattgcag tgctcaatcc cgatacttga gatattggcg ttccaattac cagcgtgcca
     8521 agctgtgttt ccttcgtcca cgtatgctcg aatttctcca ttttttccaa taccgtagtg
     8581 tgctgacgcc tcacgcgttt tccaaatatc aggaacaata tcatagttgg tggttgccat
     8641 gtgatgaatc actatcttat ctactttggc accactacga ccattcgagt aatggctaaa
     8701 tgactgaaat ccatattttg ttgctagatt actaattccc attttctgtt tcctccttct
     8761 tttcgtgtaa ctgctgcaag atttcacgca acttttccgg cactggcagt ccaatcgcca
     8821 cggcattctc cagcagtgaa attccttcgt ttgagatata gaagaaaatg accgcatcac
     8881 gcagtacatt tccacctttg atgacttcca catcaagcat atgagccaca ccaaccaaaa
     8941 gaaaaatcat gattttctta gtgatgccct ttgccccaat cttgctggat agtttctttt
     9001 cgttgattgc acgaagcaga ccagttacat agtccactac cacaaaagtt acaagcgtat
     9061 aaatcaaacc atcaattcct cccacatacc atccaatcac tcctccacca cctgcgatgg
     9121 cgagttgaat ccaattccac acttctttca tttacaaatc ctccaattgt tctaaaatta
     9181 aatcgagcat ttcagcatct ttccctgaaa tggtgacttc actattttca aatccagcaa
     9241 ccaataaagg cagcttgtct tgaagttcat ccacgttgat gtgggtttct tcgttgagca
     9301 agtctgcctt ttctttgtga tattcagttg cggtttctgg gatgagtgaa aaggcaccgt
     9361 catgttggac caaattgccg tgctcatctt tctttccgta tttttgaagc aagtctacct
     9421 cgtcttgata gagttcatca accttcacct ggatgagttt tgcaagcttc gttcgcactc
     9481 ggctaatctt tggtggtaag tcaaaacctt ttaagaaact catcacgtcc accaaatctt
     9541 catttctaat tttgagcatt atctatcttc tcctttaaat caccaatctg gacacaaaga
     9601 tcatccacga ccatcacaag caaatcaacc atatccgtcc caactttcat tggctttgga
     9661 atatctgcct cgataggttc agttactatc acttcttctt tttcttcgtt catggcggcc
     9721 tcctaactgt atgtggtcat tgaattaaac gactgcggat tgtaccaacc actcgctgta
     9781 ccgttgctac tgaaaccggt tggtaaagcg attgttcgtc ccgcaagttt ggcacagatt
     9841 tcacggatga ctccaaagtc aacccacttc ccatcatctc cgagcatcag attagaaccg
     9901 ttgaatgcaa taccagcgct gccggaactt gagccaatct tagtataaag ggaactacta
     9961 aatgtaatcc caccaacaga tatttgatgt tgtgtcccta tacgaagcat tgatggtaaa
    10021 taaatgtcgt cgtcaaagtg gaaccccttc tccataccag actgcaatgt tctcgcatac
    10081 caagaaagct tctgggtgta gacactcgca gaggaggaat ctttatgagc ccatgacata
    10141 tactctgttc catactcaag atcaaatacc aatccacgcc aatcagagtg accactgatg
    10201 ttatttgcac caattgttcc gactgtcgtt ccaccacgtc gaatggtcat tccctgcgag
    10261 tttatttcag tcacaagagt gcttcctgaa taatatccaa tcgtagttcc tgtgattcga
    10321 atggacgaac tcgcagtcaa tgctgttagg atattagcag caatcttatc agccgtaatc
    10381 gagttcgcgc caatacgggc tgccgacaaa gttcctgtgg taatcttcgc cgcatcaatg
    10441 cttgcaattt ttgcactttg aattgtggca tctgcaatct ttgcgtttgt gatggccgca
    10501 ttttggatca tggcagaact aatcactgca ctatcaatgg ttgtttgacc agtgatgtgc
    10561 accttgtttc cagcaatcaa aatactttca ggtgagatat tgatttgatt aatcacgtct
    10621 cctttaccaa ctcgtaaatt gattaagtct ttctgcatga gaattgctga atataatgtg
    10681 ttggcattgg tatagtcttg ccttgtggtc gtaggataaa tatttccttc atattcatcg
    10741 ggtttgatgc tcgtgttcac tcggactcgc attttcaatg tgccactcgg gactgtaatt
    10801 gctccgctcg aaccagtata agagatttga tgataacttg aaccatccga cgggtttaga
    10861 gtaaaaggtg tgttgcttga attataaatc ccagcatatg tgccagtctt aactgaaaag
    10921 aaagctagat acacgctgtc agtagtaatc cctgatatct gctcagccgt taacgtccaa
    10981 gaataagtgg cattgctgct acttgtaacg gtcgaaatct gttcccaagc accacctgta
    11041 taaagcaaga caatattatc tgatgtatcc ttgcgaccat tccacgaaat tacactcgga
    11101 gcgactttgc cttccaatgg caatttgtaa atgaccagat aatctcctga atttgaatag
    11161 gggtttgttg tcacgccact tgtaatactc atcgattcaa cgttcttact tactagataa
    11221 ctcgcaatgg tcgcattagt ataagttccc gccgttaggg tttcactaaa ttgttttgcc
    11281 gttgggacaa aatcaatgta aggtaaatcc atgccgtagt aatgatagta gaaagcagct
    11341 tgagttccgt cacgcagttg cccgatatac ttatctccca ctttgacatc aaggaactca
    11401 cttcggagat aattggttgc ggacacttct gaaccgtcag tggtactcaa acttccttgt
    11461 tcccattgat tgactttaaa tggaacgccg attgcttctt tggtttctga aacacttaac
    11521 agtaaggcat cagtggtcgc tgtaagtttt acgacgttac ttatctcgga taaatcaaca
    11581 tagccatttg tcacatccgt tttcaacata tagcaactga tggtttctgg tgcatcacta
    11641 gttgttaaac gaatgcgaat aaaagcggca ctgcttggaa cagtaagcgg tgtggtggtg
    11701 tttccaaatt gggtgtacga taaaaaggac ttatcctctt tgtaataaag cagataagcc
    11761 gttgcgtaag tagagtaaag tttcccaccc acaaatgttt gaagtagata cttttcgcca
    11821 tttctaactc ttgtaaaacc tgagcgaacg tatccaccat aggtattttc ttttccatca
    11881 tttgtattca gaccaccaac tgcccactgc aaatctgcaa tgctcatacc ggctaaaaca
    11941 ttgaccgctt tatttgtttt agaaagagat agctcaaatc tatcagcatt ttgggtgatg
    12001 gtgctttctg cagttgtaat acgccctgtc agattatcaa ccgtcgattg acttgcctta
    12061 ctagcaatct gcccagcttg cactgtcaga gttgattcag ctgtgattac tcgtccttca
    12121 agagttgaca taccactcgc cactaatgaa atcttgctag tattctgttc aacggatgta
    12181 gtaagcgttt caacacttgc acctacactt tcagctgcat tcaaagcatt cacagcatcg
    12241 ttaaatgctt gattggcctt ttcagtggca gtattggcat ccttttgtgc ttgttccacg
    12301 gcttgcttta cttcttctgc cgtcatatct gtcataagtg gataccactg agtgacgcca
    12361 tcacggattt catacaccca catttcaaac ttgtcaccat tttctttgta ccaaacgtca
    12421 ccgagtttcg gattatttgg agttgtcggg ccatagaagt tggtgttttt accatttgcc
    12481 gaactcaatg cgtaattggc atcgtctttg gcttgctccg ccttgttgtc cacccggttg
    12541 atgtccttcg ccacatccgt gaattttggc gtgatgtttc cgagtgttac tgagatatac
    12601 gctttcagaa atggatcgta tttgtatgag accattctag ctgtgatgtt caatccatct
    12661 tcggcatgaa taactgaaac agtatcaccc aaattaatcg tttcaagctg tgcaaaatct
    12721 ttatattctt cagttcgctc aagcggggca aactccacat cataggtggc ttttggcaaa
    12781 tcgaccttgt tagctgagta ttctaaccca gccaatctgc gaagttcatc ataggcctcg
    12841 gcttgagtaa actcatcctc ttcatcacca actttgacgt tgtcgtactt gatgactttg
    12901 attttaggct ggacgtattg gttgatgttt ggactgtcca cgtatttttc gggaagaaat
    12961 agtccatcat acccttgtgg cataatgcga gtaacaattc cagagtaatc aacatcagct
    13021 ttatatcctg tcagattctt cttgtctcgg atttgtacgc cgttgttact gccacgagtc
    13081 gttaacattg aaatcgtgaa gttatcgcgt acaatttcac caccataact gctttgaaaa
    13141 ccattctcta ggtcactatc aagcaacacc tctacaggat ttagtcgcac caagcggaca
    13201 ttattgaccg cactgatgtt tgatgtaccg gtaaaacggt gcatgaattg agttgcacca
    13261 agcagttgtg tcaaagctcc cgacccgttc ttgttgacga tataagtatc ttcaataaag
    13321 tttgtggcta ggtcatagaa aatatgatga gctactatgt gcaaaaaacc accaagtgca
    13381 tcatctcgtt caacaatcct gaaaagctgg tcggacagtt ctggtactgg tgctttcaca
    13441 atcatttctg gcaacaattc ctcactgtgt ggagcatgaa ttggataatc gaattcaaag
    13501 gaaaagagtc cgttcaattc ctcactcacc acggggttca gaatgttgcc atcaaggaca
    13561 gcgagaccgt tatgattgaa atttgattct gttttgttgt aaagcgtaat cataagtatc
    13621 gccacctcgg ttctatttct actttggtta tacctgtacc aagagtcaca gtattactgc
    13681 cgactcgaaa ctcaggaaag tcacctgtca tgtattgatt ggctgccaca tccccgtaaa
    13741 agcattcttt caaatccgaa tctagtgtca ggttgccatt cgtcagattt aacacgatag
    13801 gcttgccatt tatcgtgagt gtcctagttc cattgccgta gaccttaatc ttcggcaatg
    13861 agtaaatgtt gccgggatta ttgaccgttc ctgacgtggt tcgattgata gtcgctacat
    13921 tggtcttata acgaaaaggc gaacaggtta actccatttc aaactcccac atggaagata
    13981 aaatctgagt tagtccgctc gccttagcat acttgattgg gtaaaacacg tttggttcat
    14041 ttgaaaatgc gatagtttta gcgtttagga tttgtggcaa aattatctgc cagctagtcc
    14101 aaacatcaga tttccaaatc actaccttaa aaccgaaagt cacatctttc cagcctttca
    14161 gaatggtcaa atctccttca cgtccatcaa tctcaatact atcaatgaca cgttccgtca
    14221 ctggaataac tggcacttcc gtaattcgca aacctaaatc agtccgtgaa ttaattgttc
    14281 cgtctaaaat aaatgcgttc aattgaccac cgcccatcta aataactaaa aaaggaccag
    14341 acaacaatgt ctaatcctaa agtttataat ctatcatcct attcggttgt ttcttccagc
    14401 cctattttct tggtaatcgc taaatacgcg tatacaacca gcacgccgga aattccatat
    14461 attgcatcga ttagatctgg tgtattcaga aaagtcatca tcgtttcaca tacaatattt
    14521 gcaacaatca gaccagctcc catcgcagta agaaccctta aattcgttcc cataagcaac
    14581 caaagatagc ctatcaccaa atagaaaaga aagcttaatg cgaagtttga aatgtgagaa
    14641 ttaatctgat tgttgatgaa tacaaaacca tgatttatgc cgctatacca tcttactgcc
    14701 gttaaaaata atccaatagc aagcagtaga atatatccta tcagtgcagg tttctttgct
    14761 ttaaatttat gcttcaacat atgattacca ccatctcttc agtaaacact cgttagttat
    14821 agtatacagt caaaatgtga aaatccaaca ctaatatgct acacgtctac gattcaacgt
    14881 cgccaagctc ttatcaattt ctggagcaag tttgccgacc aatgttccat cattcaagac
    14941 gactttaatg tccattgctt taagtagcgt tggcaacatt tcactcgtga tgtttgttaa
    15001 ggcatcaacc ttagcaccta aatcaagcat cgagaatgtg ctatcaacgg cagcgtggat
    15061 atcggtatca atatcaaagc ttggcatata aagttttgtt ggaatagctt tctgcatcgt
    15121 tttagaaaca ccagtcattt ccttatcaaa acctacgcca atcccttgtg ccatgtactt
    15181 cccgatttca tcacgaaaca gtgttgatgg tgaatgaata ccaaagaagt ccttgatgtt
    15241 gtctactaca ccgccaaaga atcctgaaat cttgttgcgt aaccaacttc cagcatcagc
    15301 gataccattc cacaatcctg taatcaagtt cttcccgata gttcccatag ttgaaacata
    15361 ctcaccgaaa gcattcgcaa gccctgtgat gatttgcggt actgctttga caatggcaga
    15421 aatgattgct ggtaagtttt gaactaatgc cacaagcaac ttcacaccag cattgatgat
    15481 tttgtcgatg ttgtttacca aagttcctgt tattccgttg ataatttgcg gcatggcatt
    15541 cgcaatcgtt gtgataattt gcggtaacgc accaatcaat gccacaagta actgaatacc
    15601 tgcgtcaatc aactgaggga ttgccccaat cacggcattg atgatatttt gaataatcac
    15661 tggaatagct gcaacaatcg cattgatgat atccggcaaa gctgtcacaa gtgatgtcaa
    15721 aagttgaata ccagtctgaa tgatttgtgg aatagcactg ataataaagt tcacgattcc
    15781 tgtaataatc tgtggcagac tttcaatcag tattggaagt gctgtgataa gtccttgggc
    15841 aagtcccatg ataagttgta aagcagcatc caacagtaat ggcaagtttt caatcaagcc
    15901 gttcacaatc gttatgattg cctgtactgt aattggaata agttctggca gcgcttcgcc
    15961 tagtccttgg ataagtgttg tcacaagttg aaaggcagcg tcaatcagta aaggtaagtt
    16021 ctcaataagt gtttgaacaa tcgttaaaac ggcttgaacc accactggaa taagtgtggg
    16081 aagtaagcca agtatcgttt caagcacttg tgagaataat gaggttactg cttctaagag
    16141 tgttggcaat aactgcccca atgcttgaat aatggcatcc accactgccg gtaaagccga
    16201 cacgatattt tgaatgaccg gtagaatatt cttgatgacg ttttgaaatt gttccaccac
    16261 attgccaatc aacataccaa tgtcagcgct gctgttacca agtccagcca tcaagtttga
    16321 gattgcagac ttcataccgt caatcgaacc acttatcgtc tcactcgctt ccttggcggt
    16381 tgtaccagtg atgcccattt cggtttgaat aacgtgaatc gcttcagtca catcagcgaa
    16441 gttcgagata tcatatttga caccagaaat cttctcagcc tcttggagca aacgttccat
    16501 ttcggacttc gtgcccccaa aacctaattt caagttgtcc agcatcccgt aattttgctt
    16561 ggcgaaacct tgataggcgt tctggatgga ctcaatatcc gtccccatct tgtttgcatt
    16621 atcgctcata tcagtaacag cttgatccgc aactcctgcg gctttcttgg tatcaccacc
    16681 gagagatgaa atcaaggcag ctgagaaacc tgtgacagtt tccatgtatt cattggctga
    16741 cattccggca gttttgaaag cattgtcggc attcttttga acggtggatg acgcatcctt
    16801 gaaaagagta tccaccccac cgactaattg ttcatagtcc gcgtaagcag ataccacttg
    16861 ttttcctagc tccacggcag cagcgccagc agcaagtgcc actgtaccca ttgctacccc
    16921 gatacctttt agaacgccac cgaacttttc aaatttactt ccagactttt cagcctcgtc
    16981 acctgtatcc ttgagttcgt tgccaagctc atcagttttc ttagcagcat caacttcttc
    17041 agtacccatg gcgtcaattg ccttctcgtt gttagcaact tcacgctcca tattattgag
    17101 ttgggctttt gcattgttta attgcactgt ccaattctga gtacgcttat cgttttcacc
    17161 aaaactgtca ctagcgttct tcaaagcatt ttcaagggtg gtgatttttt ctttctgggc
    17221 atcaatcgat ttgtttaaga cttggttgcg agcagacagt gagctgatgc ttttatcgtt
    17281 tttatcaaat tcggatgata ccagtttcat ttctgaacct agcaccttga atgattggtt
    17341 gatttcacgc aacgagttct taaactcctt ttcaccttca acgccaatct ttaaaccaaa
    17401 attatctgcc aaataaatca ccaccttccc ttaaattcca ctcggtatca catcatcaat
    17461 gaataactca actttcggct tgctaatccc agtaaattgc ttatgacact cccataaatc
    17521 caaaaacagt ccaagcgagc agtaccagaa atcatctgac gtcataccca tttgaactgt
    17581 cccatagtaa tacaggcgag taaatgtttc ttcatcactt actcgcctgc tttgtttttt
    17641 gaatctgttt cactttcgat attgcgagct gtgcccttga acattgcttc agtgattgct
    17701 gatttatatt cagccaactg caacggactc gtcatcaact ccacatattc agtcgtgagt
    17761 tcatctttct tttctgtgtt cttcaagttg taaatcttga tggactgatt cgccaacagc
    17821 gtaatcaacc agatgatttc atcaagcgcc aattcaaagt tctcggattt caacaatttc
    17881 tcaccaaggt tatcaagccc accgtagtga ctggcgattt ctttcgtggc ttttgttgtc
    17941 aaaatcagtt catattcatc tccgccaagg ctaatcttgg cactgcgttc ttcactcatt
    18001 gttgcctcct agtctgctgt gaaatctggt tcataaacac tcgtgtacca tccattgata
    18061 gttgtttgtt caactcccgt atcgttttca tcaacttctg ctttccatgg atgcttacca
    18121 ctagcatcta acttgttacg acgcatgact gtaccctcaa ttgttggtgt tgagaatgta
    18181 atgtcgtcac cttttgttgc taggcttgtg ctgggcacgg caaacttcac tcggtacaac
    18241 cacaaatatt tgtatttgcc attcgattta cgagcacgga aaccaatcgc aactggtgtg
    18301 ccaccatctt cggttgtcga aattaagaca ccgttttgat ccacggttgc accaacaagc
    18361 actgccgcca cagcacgtcc aatatcatca acaccgagcg ttaactttcc atttttaaat
    18421 tctttcacta cttctgatgc accatcatcg gcgtataaag ttgcttcagc aagttccacc
    18481 gaaagctcag ctgagattgc tttcgcaagc atagcgggtg tgccgtatgt ttcttcgcca
    18541 gtagttgccg actctgtaat cggtgcataa tataatttat ccaatccaat cgttgccatt
    18601 ttcagacctc catttcataa tgcttggcga catcaatcgc cacatgatag taatttgtat
    18661 ctttttcaaa tcctacaaat aatcgttcag taatcgtaaa ctcgtgtttc aacaagttct
    18721 gaaccagttg tttttgcgtg ttcagatagt tgcctttcac aaacaaggaa atgcgaactt
    18781 cctgtacatc aacggttgga aagttatccc cgtaaacatc gaacctgtca gcgagtggtg
    18841 tcagcacaca atacttgtct ggcgctttat cactaaactc gcccgtttca acaggaatat
    18901 gaagtgtggc aaaaagactg ttcaattcct ctaaaatgtt caaatgccgt ttacctcact
    18961 ttccattttt gatttcattg tttcaatgca tgacttgcga ctttgtgatt ttgcaggttt
    19021 caagaatggc tttgctggct gaccgtgttt tccatattcg agaatgttcg caatcttagc
    19081 atttgaactg ccatcgattc gaggctcatc aaaaccaacc ttgatgttcc agttgccgct
    19141 tttatcttga cgagcttgtg tgataccaag ggctgattca agttcaccag ttgaacgact
    19201 tggtactttt gtatttgagc caacaacggc agttaagtta cttcggactt tgccttccac
    19261 cacttcagca ccactttcta aaaccttggg gatgatttca tctgtcttgt tttctagttt
    19321 tgaaaccttt aacaagaaat cttctggcat cttcatcaat gctttagcca tccgcactca
    19381 cctccatttc agtagtcagc atttcaaggt acatattgcg atttcggaca ttctccaccg
    19441 acacaatctg ataccttttc tgatctgaaa tgacaaccat ctctgtcgta acctctagat
    19501 taggaatata acgaatccga aacaggcagg tcgctgaact aaaagtcgcc atgtttaccc
    19561 atttctcagt cgcattcttt tcctctttgt atgcatggac agctgcaaga acttcatctt
    19621 gtttcactga aaatccagcg ctatcctttt ggttcgtcac tttaacgatt tgtattcgtt
    19681 gattcatctt tccaaaactc atacttgcca ccgcctatcg agcctgagta acagattgac
    19741 cgtttcccag acttgcttac tggcattgac gttatccgca aagaagccac cagttgaacc
    19801 atcacggctt tcgtaaaagt tactggctaa cacaataacc gcctgttcag tggttggcgg
    19861 catttcatgt tctgaatagt agccagactc aatatgttga tagctctcag cataagaaag
    19921 agcggtcaga atcaatcgtt ccaacagctc atcatcttcg ttatgctcaa gtatgaggtt
    19981 tgccttgacc ttatttagta agtcactcat aggctactcc ttaagatgag gcagatgctt
    20041 tcatcttcaa gatttgcact gcttcaggta acacaagttt accgtccaca cgttctttag
    20101 ctaagaaacc aaccatgcca ttacctgcaa agagttcacg caactggtcg aatgaacgca
    20161 cgccacggtc gccaatgtta tagtagctga aatcaccaaa agcaatgact ggcgcacctg
    20221 ccttaacggt tggaacaaac tccgaagtat aaactggata accgagcaga cggtcaggtt
    20281 cacctgctac caaagctggc tgccacatgt aggcaccatt gttgtctttc aacttacgaa
    20341 gcaagctgat ggtttggtcg ttcatgataa acttcgcgtt tttacgatat ggacgtttca
    20401 aagcatacac aaggttcacc acatcatctg ccgtaactgc actttgagta ttagttgtaa
    20461 tggcaacttg tccgccacct gttgctgcaa aaataccgag tggtttacca acaccgtcac
    20521 cattcaagaa cgcatcttct tccgcgtttg ctaaggcttt ggtgaattgg tcgatgatat
    20581 agttttcaag gttaaaggcg ttgtcataaa gcaattcatc agtcaccttg attgccacat
    20641 gaagtttgtg agcatcaagc aagatttgat cgaacgttgc atccccaaag ctcaactcgc
    20701 caccttcttc aatccatgaa gcggctggtt tagaacctgc aatattaatc ttgtgttcac
    20761 cacttgtcgt aattgttgtc gcaagtgtac gcatgatgtt ttcagaagtc atgccgtcaa
    20821 tcaaacgtga gtcatattct tctggcacca aataaccgcc tgccgtatcc acaccttctt
    20881 gaagaacatt tgaaacatgt cggaagtttg agcgcaaggc ttcaagtaca ccttctttat
    20941 attcagctga agcacgtcct gttttcttag gttccatttt attttcagta gtcataggtt
    21001 gcgaagttaa tggttggctc attggctttt caagttcacg atccatcgct tccattgact
    21061 gcatacgttc aatttccatc gtaaagttct tcacttttgt ttccatttcc gcataagtct
    21121 tggcatcttc ttcagacatc aagccatctt tatcttgctt tgcttctaca aaagcctttg
    21181 ctccgttcca agcttgtgca cgtttttcca tcaattcttg aattttgttc atgggttagt
    21241 tcctccaatt tttcattaaa ttaagccgac tcattaacga atcggctggg atagtgttat
    21301 taatttcttc tcgttccttt ggttcaggtg gttcaatacg acacttctta gcaatcgctg
    21361 aagtcagtga attagttact ttgacttccg agtacatcga attattaacc ggaatatccg
    21421 ggtcaatctc accgatgatt tcatcggcaa atcctaggtg aacagcttta tttgcgtcca
    21481 tccacgtctc cgcatccatc atatgtgcca gttgcacacg cgataaattc gttttacgtt
    21541 cataggcgtt gataatactt gctttcactt catcaagcat ggcaatggct ttctccatat
    21601 caactttttc gccaaacgca accgctgacg gattgtgaat catcaccatc gccacaggac
    21661 tcatgagtac cttgtcacct gccattgcaa taacacttgc ggccgaagcg gcgatgccgt
    21721 caatcttgac tgtgacttta cctttgtagt cagcaagcat gttgtaaata cttgcggcag
    21781 cgacacaatc gccaccaccc gaattaatcc agactgtaac gttacctcca cctgaattta
    21841 actcatcttt gaaaatctgc ggtgtgactt catcatcaaa ccagctctcc tcagcgattg
    21901 gtccgttgag gtagagggtt ctctctagga tttcctcttg gttgtcttga ttcagaaccg
    21961 tttgattctt ccacttccaa aacttcttca tcttgttcct cgctttcttc tttgtttacg
    22021 ccataagctg ccccggcttg ttcaattggc agcatattgc cattgaagta atatttcgca
    22081 ccagcaccac cagggattgg gttcaagtcc tcaagttcac gcacatcatc aggagatagg
    22141 aacccatttt gaataccggt tgcatagcct tgttgacgtg tggcatagtc accacgcaat
    22201 aagccatcca cattaaactt tatgaaatac ttttcttttt catcgcgact taacagtgat
    22261 ttattaagtg cttgttccca acgattgagc catggtgata atgagaactt cacaaaatcc
    22321 aaactcaaat gttcaacgtt tgagaaggtc gcgtggtcta agtcgccaac catgtgaagg
    22381 ggcactcgga atatccgtgc gatttcttcc agttgaaact tacgtgtttc aaggaactgg
    22441 gcttgttctg gtgacatgga aataggcgta tacttcatgc cttcttcaag tactgcaacc
    22501 ttggccgcat ttgaactgcc accaaatgtc gcattccaac tttcacgaag ctttcctggg
    22561 tctttcaaag ttccaggatg ttctaggaca ccaccaggat tggcaccatt ggcaaaaaat
    22621 ttagaaccat attcctctgt tgcaatcgca agcccgatag cattcttagc catggcaatt
    22681 ggtgaataac caaccaagcc atcaaagcca aggccaggaa tgtgaagcac gtcagctttt
    22741 gaaagtctga cttgcccgac atcagcttga tataaataat agatttcctt gttgtcatcg
    22801 cgattaaccg tcatcttatc tggcattaaa ggatacaaag ctacaacttc acctttgcca
    22861 tttcggataa tttgcgcata ggcgttaccc caaagtagca ggtgagtcat taaggtttca
    22921 cgaaatacaa atgatgtcat ttccgaattt ggttcatcat gcagcaagaa atacaacgaa
    22981 tgtttcattg ctttatcttt gccgccagca tctcggtatt ggtaaaagtg taatggcaat
    23041 cctgccacgg cttctgatag cacgcgcaca catgagtaca cagccgtcat ctgcatagct
    23101 gtttgttcgg tcacgctttt accagctgat gagccgccaa acataaatcg gtatggattc
    23161 atgatggttt gatttgttgg tttatcgcgt gatttgaata gtgattttat tatgcccatt
    23221 gattgctcct ttcaaaattt gagtatcaaa aaagcaccta cgatttgtag atgctgtgtt
    23281 cacattagat ataaaaccga cttcctcttg acaaaattat ctcaattgca ttatttaaaa
    23341 aggtatttac ttcctctaaa catttcgatt tttcgtcagg tgttaaggat tttctttgat
    23401 aaggaaagaa tgaactactt ggtgcaagtt ttttaaacgt catataatct tcatcatcga
    23461 agaaatcttg taaataattt agttctataa aacttttcca atctttgaat ttagagaaat
    23521 agtctttatt attaacgata aatttatcag cgttagttga tgaaccatat cgtttatctc
    23581 ttacatcaga ataaaataaa tcaggaaagt catgatacgg ccctttacct ttaacaccat
    23641 tgagagaagg ttttgataca tatagtggca taaaatttgc aagagtatga cttaaatcat
    23701 agaattttac aaatttcact ttcatctctt cggtgatatc atcggaatat aaaatgactt
    23761 tcaaacgccc ataatgacta tctggcattg aaaagttctt ttgattatag catttcaata
    23821 accgtatcgt tgcaccagca attgtgttga aagaaataat cgtttctccc ctataattcc
    23881 caattccttc agcatacttt gtcaaatctt cagttagaat ttttccccac cattttctat
    23941 aaaacagtct agaaattaat gtgttgtccg ggtctgaaga tattttatct ggtgctactt
    24001 gaatcaaata ttcattcata cacatacacc tccaaagaca attatatcaa tggattgtaa
    24061 taaaagctca tatttcacag aaacaacaat cccctatcat cataaacact ggcaccatta
    24121 tcatttccac aacgaattgc acgatccaga gccataattg tcgcgatggc accatcaatc
    24181 ttctcagtgg atttttcttt atcagctttg atattacctg ccgggtcagt tcggacgaaa
    24241 atgttgtcca tattccaacg aagaaccgga tgaccgccat gggctaattt ctgttcaaga
    24301 gtcaacttca tcaactcttt agtagccggg ctcatatcct tgaaaccttg accgaaagga
    24361 actacagtaa atcccatacc ttctaagttt tgaaccattt gaactgctcc ccaacggtca
    24421 aaggcaatct cccggatatt gtatttctca cccagttcct caataaattt ttcaatgaaa
    24481 ccatagtgga ctacgtttcc ctcagtggtt tgtatgtacc cctgcttggc ccacaaatca
    24541 taattcacat ggtcgcgttt aactcgtaag tccaaggtat cttctggcac ccagtaatat
    24601 ggcagtatca caaacttgtc atcctcatct tcaggtggaa acaccaaaac gaaggatgta
    24661 aggtcggtcg tgctggaaag gtcgagacct ccgtaacaaa ctcgacctct caaactttct
    24721 tcatctgcat tgaatgaaca agcgtcccat ttatccattg gcatccaacg cactgcttgt
    24781 ttcacccatt ggttcaggcg aagctgtctg aatgagtttt cttcagctgg attttgttta
    24841 gccgaatcac aagcagccac caccttttct tctgcaatcg ttatcccaag tgacggattg
    24901 gctttcttcc aaacttctgg atcagtccaa tcatcatctt gcttgatacc gtaaattaac
    24961 ggatagaatg ttttatcatg ctttcgacct tcaataatgt cgagagcttt ttgatgctgc
    25021 tcataacaaa ttgaattggt atcattccct gccgttgtaa tcaaaaagaa tagcggttgc
    25081 gttctagcat cccctgcccc tttcgtcatg acatcaaaca gctttctgtt aggttgtgcg
    25141 tgaagctcat caaatacaac cgcagaactg tttaatccgt gtttagagta tgcttcagct
    25201 gataatactt gatagaaact attcgttggc tgataaataa ttcgtttttg tgaagtcagg
    25261 attttcactc gtttgcttaa cgctgggttc tggcgaatca tatcaacagc tacgtcaaat
    25321 acgattgaag cctgcgctcg atcagcagca cagccatata cctcagcgcg ttcttcccca
    25381 tcaccgcaag tcaaaagcaa agccacggca gccgctaact cactcttacc attcttctta
    25441 ggtatttcaa cataagccgt attgaattgt ctgtagccat ttgccttaat cgttccgaat
    25501 aagtcacgga taatcttttc ttgccaaggc atcagtttga acggtttccc gaaccactga
    25561 cctttcgtgt gtttcaacaa ctcaataaag gccacggcat aatctgcacg tgacttatca
    25621 taatgggagt ctttgtccat aaattcagtt ggtttgtaat tatcgattgg aactcactcc
    25681 cttctacgca aaaagcagct gttgtcagct gccttaatct ctttattcca tcatatcggc
    25741 aatgaactga atggcttcag ggttgttctt gttatcccgc agcaattgca ccagttcttg
    25801 ccaatcgtag tatccttctt caatgccatc ttcattatct ggcatgtaaa tttgataatc
    25861 catcatttat caccctccaa gataaagcgg ctgtactcat ctttgtgttc ttcaatgaaa
    25921 atcaccaact cgtaaaagtg ccggtcatgt gcaattcgct gcactgcaat cgcatcaaac
    25981 atatttgtca gaccactttc tgcaatctca atgatttgtt gcttaatctt tgcgttcatt
    26041 ttgaccgtcc tttctgcctg cctcataggc cgcaattaag gcatcttcca aacaccaaac
    26101 cgcaatgtcc aagaagtctt cacggtcgct gttgtgggcc ttcaggtcgc cacgttcagg
    26161 caactgaacg atgtaattct ttgcgatgtt gtaaatctta cgtttactca ctttcatctt
    26221 aaagcccctt tctgatgtag atggtatctt ctgtttttga ctcaaggtca acccagacca
    26281 aatcgtggtt caatatcatg tccgtcacgt attgccaagc atcattgaag gtttcaaatc
    26341 cttctcttct accgttttga aagaccaatt cgaagtctgg tttgatgatg tgcaccacat
    26401 cttcgccgta taccgcgttc agtgttgaac cgttgtccca ttggataagg attgaaccaa
    26461 tgtcgtcaac accggttact gtgccttgtg tgcccttcgc tggggcatgt gggtcgttca
    26521 tcttgatgag ttctacacga ctaccttcag ggtaacgttt tttcataccc tcgatttgtt
    26581 catgagttgg aaacataact ttgtcttccg tattcttttg gtagtctata tatcactcta
    26641 aagggatttt atagcaagtg atacgttatt tattcaggat tttctaggtc attaaatgcg
    26701 atagtctttc catctcgaat cactgaaacg tctgcatcgc ttccagcctg ctcgatataa
    26761 cgcttaacaa tcacatcaca gaacttttca tccagttcga tcgtatggca aatccggtcg
    26821 gtctgttcgc aagcaatcaa agttgatccg ctgccaccaa aactatccaa cacgatgcag
    26881 ttgctgagtg acgaattggt aatcggatat gccaacagcg gaattggttt catcgttggg
    26941 tggtcaccat tcttctttgg tttatcaaat tcccagatgg tcgattcctt acgtccggtg
    27001 taccattgat gtttgccttt cttcttccag ccatagagca ccggctcatg ctgccattgg
    27061 taaggcgaac gaccaagcac tagactctgt ttcttccaga tgcacgtacc tgataaatag
    27121 aaccctgcat ccacgaatgc ttttctgaag ttcaagcctt ccgtatccgc atggaaaaca
    27181 taaatactgg cgtcgttggc catcacactt tccatacaag aaaaggcatc atacaagaag
    27241 ttgtaaaatg cctcgtcctt cagattatca tttttaatcg ttcccgcttt accttgatat
    27301 gagaccccat aaggtggatc tgtcaccaca agatttgcta gtttgccgtc catgagtttc
    27361 tcataagttt caggttttgt tgagtcacca caaactaaac ggtgacgacc aagcaaccac
    27421 aagtcccctg ttttactgaa ggctggcttt tcaagttcct catctacatc aaagtcatca
    27481 tcttgggcat catcattacc gttgagtaat ttatcaagtt catcagtttc aaaaccaagc
    27541 aaatccaaat caaactcttc accttgcagg tctgataact caaccgccaa catttcatca
    27601 tcccaaccgg cgttcattgc tagtcggtta tcagcaagta tgtacgcacg cttttgtgcc
    27661 tcggttaggt gttccacaaa cacgcacggt acctgcttga taccttcttc tttagccgca
    27721 agaatacgtc cgtgacctgc aatcaagtta taatccttat caacaatcac cggatttatg
    27781 aacccaaact cacgcataga agcttgaatc tgtttcactt gatccttact gtgggtacgg
    27841 gcatttctgg cataagggac cagcttatca atatcaactt tctcaaaacg ttctgttgtg
    27901 ttcattagaa caacccccat tctgctagtt tctcaaaccc gccaagctca ttcacataat
    27961 cacgcgcaac ttccacgatt tcagaataag gcacgccatc aatctcagtg tcaccaatcg
    28021 cacagcttaa ctcaacgggc ataccagttt cttgagcttt caagaatgcg tagatgttca
    28081 ctgacacatc agcttttgat aagtctttcc catggatacc gccacctgtt actccgtctg
    28141 ccatatcgga accaagcttg cgattagtcg cacctgtatc cacatccggg ccgccagtcc
    28201 aatcacctag agggttgact gtagcgtttg gatatttttg ttttaactca tttgtctttg
    28261 cgtggctctg acaaattatc aagcgttcac catcaagaat gtactttcca tcaaatggat
    28321 ggtcctgata taagtcacgc gcaatttttg agagctgtaa ctgttcatca gtcaatggca
    28381 tcccacgaaa gattccatta tcgccacaac gaatttcagt cgcttgattg tcagccaact
    28441 ctttatcttg gggcacaatc accacatctt ttttaatttt gcctgcgata cgattaatcg
    28501 cttggctgat ttcattcaaa tttaaagcag ttgaagtttc aataatcaca tgacagatgc
    28561 catgaccaat taaaacctca actgctatct ttggattatt ttcttctgcg tatgccaagt
    28621 ccacaatggc tccggcaata cgatctgcaa ctttatctgg atgttgcgga ttaaccttct
    28681 ctatcataaa ttctgttcct ttcaaccatt cttagaacgt aacaatcgtt ccatcgcatc
    28741 cgtattcgga tctcctgtgt attccactga tgcattctct tttacaatct gttgaatctc
    28801 ataccacgtc acatttgctt gcttttggaa gttctgcgac atcgccacaa atggggaggc
    28861 catgactgcc ttggtagtcg ggtgttttcc gagcaaacca tattgactca gagcattttc
    28921 acattgaaca aaacgcgcaa agttctgagc gtaggactca accattcgtg gggctatcaa
    28981 cgtttcgcat ccgcggtctt tcaaccattg gaacgttgag ttataaattt catcagcgcc
    29041 tagcacagtg ccatccttct gttttgcaga aaggtaagca cttggttcag gataatcatt
    29101 gccctcaagc tctacaccct caccaatatc ttcaccaatt aataattcaa catccggtaa
    29161 gtcaaattcc atccgcgatg ctttctttcc agcagcaact ttatcaacga gtggctgtga
    29221 gcgttcccct gaacgaggtc gtctgccacc gcgttgtgtt ccatcttttg ccaaaattta
    29281 tcacctcttt ttcttgtttg gggctatccc ccgtttgaac tgaacttttt tcgcgtaagg
    29341 gcctagcgcg ctgtacagtt tagaagggtt tagggataat taccgcccct cccccccttg
    29401 ttatttttca catgattgtt ttatcttttg tgtttcgtcg agtcatgtga attttcgagt
    29461 gacatgattt gcagacactc atcaaattat tttcatcatg cgtaccacca tcacttaatt
    29521 ccttgatgtg atgcacaatc tcaaccgaag taaagcgtcc ttccttcaga cacatctcac
    29581 ataggggatg tgctgccacg taccttgccc gtattttctt ccacgctctg ccgtaacgtt
    29641 tgttgattgc cggatcacgc tcgtatttct cgtaacgttt cctcgcaagt gttttatgtt
    29701 catcacaata agtatcatgc gtacggttcg gacaacctgg gaacttacag gggttagctg
    29761 gtttcattgg caagtggggt cacatccttt cgggcaaaag aaaagccctt gaaggatttt
    29821 tcccacaaag gcttcgagta tttgttcgat acttctattg ctggtattgc ttgagtatcg
    29881 tttgtctttt ttgacatctt aatgatatca ctaaaggcta ctgacattca ctgacacatt
    29941 tcaaatgact atgggttcat ctggtagctt caagttctca agagctttgc tatgccaacg
    30001 aaagactgtc cgcacgtcag cgtgaagatc ttcagcaatc ttgtcccagc tctcttccaa
    30061 caggtatcga ttgattaaca caatcttttc gtccacgttt gtgagttcat caatcacgcc
    30121 acgcatttgt cgtttgaggt ctacaaactt atcaatctct gcatcaattt cagcttccaa
    30181 ttggtaacac ttctcaaggt tacgaacaaa tggtgcctca gtattccgtg tgccacttga
    30241 aatcttttct tcgaatgttg tggaagaaac tgtcaccgcc agttgataga gctccgcctt
    30301 ctctttaatc cggtcgtcaa ttcgtttatt caaacggtac gcttgtttca agtattcttt
    30361 tggtgtcact tgcttccctc caatctagct ttcactgcat caatcaaaca tgattgtgtt
    30421 ttatcttttc gtttcaaagc caacatcaca tcttcatcaa tcgtatcttt cgcaataatg
    30481 tgatgaatga ccacagtatc actttgccct tgtcgccaca gccttgcatt ggtttgttgg
    30541 taaagttcca aactccacgt aagaccaaac catacaagtg ttgaaccacc agcttgtaaa
    30601 ttcaatccat gtcctgcact agccggatga atgacagcga tggggatgct accttcgttc
    30661 caatcaataa tgtcctgaga cgttttgatc tgtcgaacgt tgaatcgttc cttgatacgc
    30721 tccagatcat gctggaacca ataggcaatc agaatcggtt tgccatttgt accttcaatc
    30781 aaatcttcca aagcatctaa cttctggtca tggatgtgat gactcacatt aaactcatca
    30841 tagattgctc cattcgccat ttgtagcagt ttattcgaaa gcaccgctgt attggctgca
    30901 tcaatggtgg cttctttcaa ttgtagcacc aagtctgcct taatttcatc gtacttttgc
    30961 ttttctttga gtcccatatc aactggcact tcattcatca caagctctgg catatccaag
    31021 aaatcgactg acttcattga aatcgtgata tctgaaatct tgtcgtagat ttcttgttct
    31081 gcatcttccc ttggtttcca agaataaatc tgcatgccat tttgcttgtc aggtagaaag
    31141 tatttatctc gataacgtga aatataatag cccaatctct cgcccatatc cagaaccttg
    31201 aactccgcaa acaaatccat caaaccatta cttgaaggtg ttccggtcaa tccaacaatt
    31261 cggtcaacct tgtgtctgac tttcataaag ctagtgaacc gtttagcttt taagtttttg
    31321 aaggacgaca gttcgtcaat caccaccata tcaaaatcaa acgtaaaacc cgatttgtta
    31381 atcagccagt ccaagttctc acgattgatc agatagatgt tggcattatt ttgtaatgct
    31441 gccattcgct gacttggtgt tccaagaacc acggaatacg ataaatcttt caaatgatcc
    31501 cacttttgaa tttcagttgg ccatgttgat tgcgtcacgc gcaacggtgc aataattaag
    31561 acacgttgta ttgtaaagtc atcatgcatc aagtctttaa tagctgtcag cgttgtcacc
    31621 gttttaccca agcccatgtc caatagaagg gcggcagttt tatgatcttt gataaaatca
    31681 atcgcatatt tctggtagtt atgtggtacg aacttcatca gacatcacct cctattcttt
    31741 ctagtaactc aggtatctga tcaatctcat caagcacgaa acactgaaac cctaaatctg
    31801 tcagttgttt catacgaaaa agctgtaaag accgtggctt cttccctggc gccttcactt
    31861 cgacaaatcc aagtttccca tttggcataa gtaccaatcg gtcaggcaca ccagcaagcc
    31921 ccggtgatgt aaacttcggg caaataccac ctaccctttt gacggctttc actagtgctt
    31981 gctcaacctg cttttctctc atagccacac cgtcaaatac tcgaaatacg catcacgcaa
    32041 gatttccaag tattcctttt ctgcgcagtg aatcatcaga tgtgcgactt gcttatcgaa
    32101 tgatttcttc caaggatacg tgacatccga catggcaaac tcgtaaaact catcaaatcg
    32161 taaatcacgt ttaccgattc caatttgtaa ccactgttta aatttcattt agatattctc
    32221 caatctgcaa gtatggtgta acccgtgaat acgcaataca taactttata taggtggtaa
    32281 ttttttatcc cttaagaaaa gttctatata agacctacac aggttacact ttcctttaaa
    32341 atcaactatt ccaagaactc ggatttgagt tgcaaaccaa caatcttgtt tcctttatta
    32401 gttcgccggc gcttaaatcc gcccgcctcc agagccgtgt aaaaatcggc tgaactacga
    32461 atgtactcac cagttcgcgt acaaaacgca cgatactctg catacaactc accagatttt
    32521 tcttcaaagt caacagcagt ctcgcagcac tcgtctaaga aatggccaag ccaatcattc
    32581 gccgtcttat attcagaaat tgcattagcg acaacttgtg gaaccgttaa atgaaagtct
    32641 gcggcgatga cttttttcgc accgtcaagg atccattgca aaatggctcc gccggcattt
    32701 tcaaaaaggt agtccgcata attcttgatg tccttattgc cgtcaatcgt agcaaggaat
    32761 ggaatcacaa ttaaacgacg ccaggttcct ttatcaatcg ctccaacttt tgggaggtgg
    32821 ttggtataga gcaccaaagt atgcgttggg acatacttga atgggtcttt gtatttcttc
    32881 tcagccgcaa tttcatccgt cgagcagagc tgcttaatgt tagatgtgtt gagtcgcatc
    32941 ccctcttcaa gctcggctgc gatgagtagg cgtttcccct tcgcttccgc aagttcaggt
    33001 ttcacattgc gtcggatttg acttgtaaga atatcagctg agatgctgcc agagtaatta
    33061 ccgagcacac ggctgatgac attccagaac gtggacttcc cgttacgccc ttcaccatac
    33121 gagatgataa gcgcttcaac gtaaacttta ccaatagctg caagtcctac aatcatctgg
    33181 acataatcaa tcaactcttg gtcacctacg aaaatggtgt tcagagcgtc caaccaaatc
    33241 tgctgattgg cgtcactcgg ggcacattcc gtttgcttcg tgatgtaatc ttctgagttg
    33301 tggtcttgtg atttgcctgt tcgcaaatca aaggttgctg ttggtgtgtt cagcaagaac
    33361 tcgttcacat ctagtaaccg ttgctcgatt tcaagcatgg gctttgcctc ggttaatgct
    33421 gacgagagat acttgctgtc gcggcgtttg acagcaaact tcttatactc ttgtgcgtgt
    33481 tggtacaaat caaaactatg acgttgcgct gtgttgaaca tggtgactgc ctttttcgga
    33541 ccaacactag caagtacatc gaacgcgcca ttactaacca tttccttggt acgcttctca
    33601 atttcggttt cagattcttc cagttgacgt tctgtcaatg cgtggatcac accttgcgct
    33661 tttggtttcg actcttccca atagctgccg ttgtagacaa ggtaaccagt tgacggtgag
    33721 taatgaagct tacattcata ctcgcgagct aagaccgttg cttgacctaa gtccgagtag
    33781 tcggttggct caagtgataa atcagagttg tactgctctg gaggaatgta gccttcttgt
    33841 tgtttcactt tcttcccaaa gttgaccgcg ctattccaga tagctttaag ctcctcctcc
    33901 ggcagtggcg gattacattt ttgggattgt tcgagatact tgtgatacgt atcttccgtt
    33961 gcaccgtagc gtttgatgag ctttcctgcg atgtgactca tgcttgaatt acggctgcct
    34021 tcgctaatct ctgactgcgc attatcccaa tcagcaaaca gatcatcttc gagaaaatca
    34081 gtgatcaact tactaccttc atagatttca acttcagagt ttggtgttcc gaaaagcaaa
    34141 cgtgccgcat ccatcgcatt gttgtcaaag tatgggaact cactttgaat ctgcttcttg
    34201 agttccacat attcatcttt atcagttgtg gcaggaatcg ggaaatatac gtgaaaacgt
    34261 ggacgtgctg atttgccacc tttgtctttc atgttgttac gtgaatagct gaccgcaaac
    34321 gtcacatcag gaaactgcat cgccacttcg aaaggggcta cccaatcatc tggattgtct
    34381 gagtggtcat tgtcgcaatc aagtggaatc acatcagact ggatgaagtt gtcacctttg
    34441 cgatagttgc ctttgtactc agccatcaca tgatccagct tgaccgtggc aacaagtgtt
    34501 tctttatctg tcaccacttg cttggttggg aacaatccat tcttggaatt acccacctga
    34561 ttggatgtga ataacgtcat cttaagcatt tgagacctcc ttcatctcat catcgaagta
    34621 cctgatatgt ttcttccaat actccgcttg ttcaatttca actgccatgc cttgggagat
    34681 ggtgtcaccc accacccaga cttcctcaca tttgcccatg agaacaagtg acatgtgcat
    34741 tgccacatca cgctccgttg cgtcgtccat gaattgtgga tacatcagat gtgccgcaac
    34801 tggcatgtag ccacggtcaa gagcaatacg gcagtaacgc ttagtgtttt tatggttgac
    34861 gacaacattt cctgagaacg gcgagcagac atagactgat gttctgtaat ctttacgtct
    34921 aggcattatt atcacctcgc tcaatcactg gtaagatgtt cttcttcgat ttcagcaagt
    34981 cgtacaagaa caaacgacct ttctgcgtcc agtaggtatg catcacgctg cgctctgcat
    35041 ctatcgcatg agtttttgat tgtgtgtagc cttgattggc atacttctga taaagcaacc
    35101 acatcttgcc ttgcttgtac tgaattccca gttcatgaag caacttgttc attgcctgtg
    35161 gtgacatacc gtagtccttc gagattttag aaatcgacat cacagaattg ttttgaagaa
    35221 tcaaatcgta gtagctaact tttggtttca tctccaaaat catttgttgt tgcatcgcca
    35281 ccatttcttt tttctcttgt agctgcttca acactgaaat ggcccatgct ggatcttcga
    35341 ttgactttgt gatgaaatct tctgtcgcgt acacaccgtg ttggcggata gacggcaaga
    35401 cctcatttgt cacccaacgc ttgaatttct tcgcgttcgg catcttgcta gaaatgatga
    35461 gtgagtacaa tccactttcg ttgataagga tagccattgt cccattaacg ctgaacgatt
    35521 cgttcaccgt cttatcttcc tcatcaacat gatctcgtaa tgctttttgt ggatttgtgt
    35581 agccaagaat tgatgctaca tctttaccaa caaagtgagg gacatcgcca atcatcaccg
    35641 tgcgtacaga gccaaactcc gcattttcaa aaactttcaa ttcgttcatc ttgtgaacct
    35701 cctgtaatag aattttcagg agaggcattt tgccccttct aacttacagg cgaagaaaat
    35761 ggtcgaaagt aaactctgac tcgaatttat ttttgatttt tttgccttcg tctataagcg
    35821 aagaaagaac ccaattcgaa ccttattttt gataaaaaaa tttgaaaaaa atatggagcc
    35881 atttctgact ccacattgac taatcctttt gatagaattc acactcgtat ccatcagcgt
    35941 tcagtagcaa gccttcagcc cacttaggcg tttgactcat caactgacag atgtcttcaa
    36001 ccttcgattc cattggcact tcgataatca cttcgtcgtg gacgtgcccg acaatgcgat
    36061 acttacgaag attctccatt gcgaaacaga gaatgtctct agctattgcc tgcacgatgt
    36121 tttcgacaaa cttgggacca tagctttcaa gacgttccca tttcttggta gcaccgacac
    36181 cttcataggt aacagcttca ccgccgaatt tgttctcacc aatctttggt tttacataaa
    36241 ctaatttacg tcctgacaag agttggatga acagaaaacc cgacttgtat tcaaacttga
    36301 tgccatgcgt cgatgtgctc cgtcgttctt tcacacaagt ctttactgcc ttatcaacag
    36361 cccaccaaag ctccgttatg ttaatgtttg agtctcgcca agaattaaca agcggctgta
    36421 attcatcctc agttagtccc atttcaagag catccattgc tttcaatgca cccaccgatc
    36481 ctccgtaccc tagagcaagc tcagcaattt tccctttttg acgtagatgc ccattcacgc
    36541 catgcttttc aactggcaca ccgaacatct gactggcaga cgcacagtaa atatcgccac
    36601 catttttgaa tacttcttga cgccacttct cacccgacag ccaagcaatc acacgtgctt
    36661 caatcgctga gaagtcagat acgataaatt tcatcccttc acgtggtaca aacgctgtcc
    36721 ggaggagttc tgagagtacg ttcgggacat tgccgtagag aatgttaagc atttcaaaat
    36781 tgctttgttt tactaaacta cgcgcttcag ataaatctga catatggttc tgaggcaagt
    36841 tttgaagctg gatgccacgt cctgcaaagc gtccggtacg gttggctcca tagaattgga
    36901 ataaaccacg agaacgatta tcagaacacg caaagttttg catggcttga tactttttca
    36961 cggatgactt ggcgagttgc tgacgtaaga ccaggacttg ctttaaattt tcatcaatag
    37021 gttgttcgag tagttcggcc actgcctttt taccgagtga ctcggtttca agtccgtggt
    37081 ctagtaacca tccccgcatt tgaagcacgc tgtttggatt ttctagtcca gttaattctt
    37141 gcaattgttg attcaatcca gcttttgata attcatccaa ctgaacggca gcttccacaa
    37201 agtccttatc aatctcaatt cctcggtcgt tgatttcctg atccagccag tattctttcc
    37261 aaacagaatc aagcactgga aaattggaaa gttttccttg gatagctatt tcaacttcca
    37321 catcacgact attgtatgtc ttaaatgctg accatttagt tggcgcatgg tgtggtaagt
    37381 tcctcgtgcg tccaccatta actttggtcg gattgcatgg cacgcaaaag tatcggatga
    37441 ggtcttttcc ttctgtgagc ttttgtttat ccaaaccaag aacagcacca actcctacaa
    37501 gtgacaacgg caaacctaat gtcgcacccc acaccattgt gcattgccac cctcgtggat
    37561 tcaaaaagcc atccaacccc agaaagcgtg acaaacacac acgttcaaaa ctcgcattga
    37621 aggcgtgttt aatcactgtt tcatctgata gcgaagttaa tatttcagcc gggatagttt
    37681 cgtccatctt cagatcaacc acttcgacgg gaccaccatc tacactgtat gcaaatagca
    37741 gtatttcaaa atcgggggac tctgaatatt tgtagacacc acatttcgat aaatcgacac
    37801 tactatacgt ttccaaatcc aatgacaaat gtttcatttt cttcctccaa tcaaacaggc
    37861 gacagcactt ctgccatggc cgtttttgtt ttaatgatta agccaagaaa tcatcatccg
    37921 ccaaggtttc aaactcatcg cttgcagata cttgtgagcc gagtggttcg ccgtcacgta
    37981 caaactggat attcccaagc cctgccgcta ccccgcgatt tccatttgcg ttaaaggcat
    38041 agaaattaat cgacacacga gcgtagcaac cactatacac ttgtgaacga tccaagattg
    38101 gttgaacgtg ctgatccacg atttgtggtg cagtggttga gttcgcatta atgaagtaat
    38161 ggccttggta tgcttcatcg tcgcgttcga tatccccatc acgtaatggc agcttgattt
    38221 ctgctttgtt tggtttcttc ccaccgaatt tcgcaagtcc ctcttcgatt gctgcatcga
    38281 ttgctttttc aatggtatca atcgttctct tatctgattt tggaatcaag actgctgttg
    38341 aatatttctc tgcgcccccg ttgatggatg ctggttccca accgtggaag tatgagagac
    38401 gtgtgtttgt accagtgatt actttgcttg ttttagacat attattgttc ctccgttaat
    38461 ggtttaaatt cttcgtttaa gttgtttact gtgaccgctt gtcgtttatc tgaaccatcg
    38521 actagcgtca gcttgccttt tggtttgatg atgtattcgc ccaagatttc attgaatttc
    38581 tctttaccga gtgccttcgt cattgctgtg acaccaagta actttttatc atatgggtcg
    38641 tatccagcct tgatgaccgc cgcagcgaca tcattgtcat tagaatactt acgagtagag
    38701 cgaccctcga ctaatttgtg atgagtccat tgtttgccac cgagtgcaag tttcaaagca
    38761 cagtccttga tatcgttact ccaagaaacc aggtcatcca ctttaccgat gatttcttcg
    38821 atttcatcat cactaagaag ctctggtggt ttgaactcat attgcgccag tttcaagtta
    38881 tggtctgctc gtgcacgaca gctattcttc gccggacaga atccacacca tttcccgcat
    38941 tggtactcac cctcaccttt gaaagctaaa tctgcggcag gcgacaacac atcttttgcc
    39001 cactgaagta attcttcttt cagcagtgtg tacgtactca cattctcacg ccttggctgg
    39061 aaaatggtca tcttgacttc ttgaatgtca taaataacat cgaaaagctc caacgctccc
    39121 aaggcataac acatcatctg tgggttctga tacgcatcta ccaaaattcc tagaccgtat
    39181 ttcatgtcaa taacagatag ggttccatcc gcaatgacga gacagtcgcc ggtcccaaag
    39241 ccgtctggga cgtaccttga gaagtcgagc cgctgttcta ctattacaat agagtcaact
    39301 gttgtctgtt tcgccttagc gacttcttcc atgacgaagg tcgcataact ttcagcacac
    39361 tgctccatct cctcgtcgta gaattcaagc gtagatgttg ggtctgtgac gtcattacca
    39421 atgaacttat tcagtttgta ctcacataag gtatgagcat cagtgccttg ttgggcatac
    39481 gggctcgact tgttttcaaa gtgctctgac aagcgaacac ttggcggaca gtgaatccac
    39541 cgattacttg aagatgcgga taggactgcg tgttgtgttg gcatctacaa tccctccgca
    39601 tctgcaacca aagccgcgta gttttctgtc ggcacatcac tcaagcgact ggcaccatat
    39661 ttctcaatca gtgctttcac ttcagttgac ttaccttctt gagatttacg tgccaacaaa
    39721 ccacggactt cttcaagcgt cggttgtttg acttctgatt ttgtttccat tgtcggttct
    39781 ggtgtttcaa cttgtggttc aggttcattg gttttcatcg ccacagccaa ctcttgtaag
    39841 ctatcagcta acgcttggac gtcctcaacg acctgaagaa atagttttgt tttactcacg
    39901 tcattacgct cccttcttgt taacttcacg gatttcgact gattcgattg tgtcccctgg
    39961 tgtgagcacg agaattttgt ccatgttgcc cttaccgaac agccaccgtg caaaccagcg
    40021 aggcaaatga cgcacaccac cttgtaggac agtttctttg ttacccgtgt catcagtcac
    40081 gtttactcgg attttgtgtt ttaggttcat gatggtcctc cttcatcttt atttgtttag
    40141 gatctactcc cttacacaat acaggcgaag gaaaacgggg aaagtaaact cccccgtaac
    40201 ttttttctaa aataattctt tgatcttgtc tttgatgatt ccaaaggtgc gattgattga
    40261 ctgttttttc ttgtcaagct tgtccgccac ttcagtctga gacatgtttt cagtgacgag
    40321 catgtgatag atttcttggt ggtacggtaa taattgcgtc acgacttcac gcaaacgatc
    40381 gatacgagga tcttcttcat tatcttggta agcatcgtaa accaatgatt ggatggggtt
    40441 gtccccctca tcgcattcat catcaaattc attgagcgac aaattgtagt tacgtttgaa
    40501 gtaatcgttg gcttcaccat ccacgtattc gtccattgga taatagccgt gctcattatt
    40561 aaagtcttca cggaacttct ctttccaagc cttgatcttt tctttttctt cttctgtgcg
    40621 ttcaggacgt gcgtttttat aaaagttgta aatcgcacgg tcgtcagcgc gatgcagctc
    40681 ctgaatgtta agctcagtga cgccgtcttc gcctgggcga atctcaacca cttctttttg
    40741 gatacgtcca ttggcatcag tacgctcaaa cacgtacttg taagtgctgc ggtcgttttg
    40801 gtgtgttttc ataaatctac tcattagatc cctctttctt gcctgaaaga gcttttgagc
    40861 aaggaaaaca aacagggacc gtgccaatac gtacaccgtc cctcatcacg aaaaaagagc
    40921 gcaataaagt aaggcataca atatctgaaa aattttctgg ataaccagtt cttttcagta
    40981 tttgtaacct tgccctattg caactcaggc attgttatat tttattagta ggactattcg
    41041 cttttaagat aaattcgttc actttcttga aacataatgt agtgtttatg aaccataaat
    41101 gataaaatta aatagttaaa ctactatgtt ttaattatag aaaaaggcct cccttgtttt
    41161 cgtcagtcat cggcggcaaa cggcggtcaa cggcggacgt aacaaaggag gttgtatttt
    41221 ggaaataagt gaaattactc aaacgcttta tccgtttatg ggaagcgagt acgtgaagcc
    41281 acaggatttt ttaattgctt tactggatag tgtttcaaat gatgacgagg ccaccatttt
    41341 cggtttttct gatgataacc tacgaagatt ttacaatggt actcgaccat taacacaaga
    41401 gtatgcagtt cgggttgctg gaaaattcaa catcgacaaa tgcacgactt ttatttatga
    41461 acgtcttata gatgatgctc caaccgtcct taagcaacaa ttgattgata aaggtgtggt
    41521 aatccccaaa gatacagacg tttctgaaat agtggcccat cttttacttg atgaaatcac
    41581 ctcaattgct tctcaaacaa aagtaaaaac aaaaaaggcc aagccgaagc ttagcgaatt
    41641 agagctcgca agtttaagaa tcaacactaa tggtgatttc tcttataatg atgaagtgaa
    41701 aaaattcttt gacgatttgc cagttcccct tgatattaga gctgaggaga tgacgtatgt
    41761 catagctctt ttttcagcat atgcagatgc cgatggatac gaagtatacg agcatacaaa
    41821 cgacctgcca aattcgcggc taaaggattt caagagacag cgaagaaatt actataaagc
    41881 tgaaagcata cgtcgtgggg tccgtgataa ctttacacaa gaggaaggaa ctgagcactt
    41941 tgaagcttta aaagaagata tgtacgacgg catcgaggaa gtctatgaag aagactacga
    42001 tgatggcgtt gagagactca aagccgttct acagcagtcg tctgttataa cattaaacgg
    42061 atcaatctta tcgtttatac cgggattgct gcaaaattct gctaaaaaag gcatttgcca
    42121 catgctggtt aatgacggaa agatcaaatg ggtgactgaa gatgaatagt ttgtttaata
    42181 ctccatttga aatttcggtt cgagtaatgt tactgctctc tgaatttaaa gagcctgtca
    42241 gcgctgatag aattttaatc acagacttca tcaccacgta tggtcgcgac tttgaaattt
    42301 ccgattacaa cttaaatggt gataacagct atagattcag tgaatttatt gcgcgacgtg
    42361 aattagttaa agatgcagtc aaaaatcttg tactgcaaca tgttatccaa ccatcgcaaa
    42421 gcaaagaagg ttttaagtat gctttatcag aagatggcct aaagttgata tcacaattcg
    42481 tatcggtata tgcagaaaaa tacttacaaa tagctgacga atctgttaaa tacattcaag
    42541 ataaaagtga cgttgagctt ctgcgacaaa taaaccaacg cgcaaaggaa tttaaaggag
    42601 agcaatgata atgcaaaatt attatattga acgtgttacc ataaaaggca aagatcgtga
    42661 agatgcaact gtcaagttca ccaatggact aaacataatt tctggccctt caaataccgg
    42721 aaaaactagc attgttacgg caatcgattt tctctatggc gcgaaaaaag acgctgctcc
    42781 cttttcaacc gatgcaaccg gatacagtaa agttgaactg attattcaat cgactaatgg
    42841 caaatattca ttaactagag aatttgatag caactccatt ttggtatcca aaataacgag
    42901 ttcaggtgaa tatgtagatt atgaaaaata taatgcacga tctgggaaaa atccaattag
    42961 ccatttttgg ttacaacttt taggcatcga tgatgattat cagattatca agaatgagga
    43021 tttcaaacgt caaaaattaa cttggcggac attagcgcct agcacactta ttccacaaga
    43081 tagagtcaat accagcgcct ctgttctttt tcctggggaa acaacccaac gcacagcttt
    43141 tttatcatcg ttgttacttc tgctatacgg ttcagatttt gcgaactcgg aagaaaacga
    43201 aacaaaagaa cataaaaaaa ttagaaggaa ctcggtgatt aactacatca attcaagcct
    43261 ttctgaatta tatgaaaact acgaagtggc taaatcagaa ttacctgagg ctccctctga
    43321 tgattttcaa caaattgtcg atggaatgtt cgctgaactt gaaacaattg aaaagcaaat
    43381 caatgatgcg ttagccagca gcaaaatctt gataaaagag atttatcaaa aggaacagta
    43441 tcttcatgaa actcaacttc ttttgaatag gtacaaacag ttacattatc aatatcaatc
    43501 ggatattgaa cgactaacac taatcgtaga tggtgagcga ttattaaatg atcataatga
    43561 acatgatact tcacatagct gtccattctg taacggcgaa ctaagtaaac ctgtccaaac
    43621 aacctacatc gatactgcta ggtcagaatt acattcattg ctctctcaat tatcagattt
    43681 gacggaagaa tctcatgata ccgaagttga actgcaagag ttaatggaac agcttgatga
    43741 gcttaaaaca agaaagaaat cgcttgatgc agcaatcaac gatgaactaa aaccgaaagc
    43801 tgagacgtta aaggatcgaa tcaataaaat cgaaacttac agggatatta aacaacagct
    43861 aatctcgatg gaaaaaatgc aaaataagct taaaagtgat cttgaaaaaa tcgagaatga
    43921 gccagaagac gaacgtatcc tttataaacc taaagagaaa tttgatgcag aattctacaa
    43981 agaaatgtct gagttactgg actccatgtt acgtaatatg cattataaac ctaatgaatt
    44041 tcgtaacgca tttttcgcca aggatatttt cgacatcaaa atcaatggaa atctaaaatg
    44101 gaccgaaaac ggacatggat atacagcgtt tatcaattca gtagtggtaa tggcatatcg
    44161 acatctcttg tataatcacg ctaagtacac accttctgtg ttcattatcg attcccctct
    44221 tcttggatta gatgaacgca aaaaacaaga tggcgatgac aatatgaaag aaggactttt
    44281 caaatacttt atggatcatc aaaacgaagg acaacttatc atcgttgaga atacaacgaa
    44341 cttgccagca ctaaactatg atgataaaca agtaaatcag attgtcttta ctgaagataa
    44401 aaacaatgga cgctatggat atctatacga tatttataac tagaacagga atgattacat
    44461 gaagtttagc tacaatccgc tttggaaact cctaatcgac agaggactga ataaagcaca
    44521 actcagaaaa atgactgata tcagttcagc aacactctca aaactaaata aaggcgaaaa
    44581 tgtaacaaca gatatcttac ttcgcatctg tgaagcactt gattgtgatt tatcggatat
    44641 tgttgaaatc gttcgcgatt aaatatcaaa aatgcactgt attcgattca tgagtacagt
    44701 gcattttcat atttattttt gtaatccagc gattacttca ttgattttcg attcaatatc
    44761 attcaatgga accatatacc attcagtagc ttttttgaag tttccatcct gaaagacaac
    44821 ttgtttgtcc tggaatacat gatggagagt gcgctcaagt agctgagcgt tgacattttg
    44881 tacttcagca gaaagcatga ccttcactgg tgcatttaag taggtcacgt cgttttccgc
    44941 attggcaatt ctactttcaa catcatttcg agtgaaacca atcttataca ggttattcca
    45001 cttgctaata tccggattcg tgcttaatga cttcagcacg tagatataac ctgtcgtaaa
    45061 ctcttctcca aagctttcag ccagtgtttc ctcatctacc ggctctgtca ccatccttcc
    45121 atgacgttca gtcgagtata gagacgaagc taaagattgt aacaacagat tcttattctc
    45181 tgtcccattc tcataaacaa catgcattct ggcatttgtt ttcccattat cagcgataaa
    45241 ggtatccttc gaaatggaaa cgatatacat cattacgccg ttttggatgt agaatctatt
    45301 ttcatgaata tcatactgct caaacgggat aatttttcgt ctgccagatg cgatatctgc
    45361 atgcacttga tcgaacaagc tcttatactt ttcaaaatga cttgcatgtt ttctgcggcc
    45421 aattttatct gcagcgttca cagttctacg atagcgggaa acatccaaca agtctgaaaa
    45481 atcatcaatc atatcatctg agcctaagat atcatctaaa ttcaactcgt catcgatttc
    45541 aactgaatca gcgagaaact ctccatccgg ttcgaattct ttctccaata aaccaagagt
    45601 gtcatacttc ttaaccttct cagacctttc gggttttcct cgaaatccgc taagacgagc
    45661 ccacaatgcg cgttcactag accattcagg agttttttct ggctctcgtc cgttttcatt
    45721 cacaaagtct ataatctcct gaaatttagc aacttctggg tcttgtttta caactttctt
    45781 tttttcaact tgcttggtta aatcatcaaa agcgtcatct tcaaagatgc tatctaaact
    45841 attcgccatc ttctttcgct gcctctcttt cacgttttat ctcagcaagt cgtagcaaag
    45901 caaaggccaa gtatttttca ccatcatctt tactatttcg atcaggattt cgaccatttt
    45961 ctgcacggaa cttcttagca gcttggtagg ccgcaaccaa ttgttcgtca gtaaaatcat
    46021 acttatttga tgacaaataa tcctgaataa accgaagagt cggtgcatca atatcacgac
    46081 taatcacttc atacgcacgt tggaacggat tcacttgatc gattaaatca atattcaatt
    46141 catcaatgtt aatgaaacga tcggccatct taataatttc gcggctacca tcttctgaag
    46201 ttgttgtccg tccagccgca atatttatat cggcaactgt atgttgacga acggtttcta
    46261 actcttcgtt cgtcaaatca ggataacgat caatgatgac acgtggaatc aacgtcttat
    46321 taatcatctt cgcatcagca cctgaaacaa tcgcattttg aatatcttgg ctctgtaaaa
    46381 tgctagattt caaatcttcc atatcatttt cgatgatttt ctttgtacgt tccgtcgtat
    46441 cagcaggttt cagtcctctg attgaaagat cacctggttt gtctggctta ccatcttttg
    46501 gtttaaactt gtaaactggc gccaacactt gttccataag tagagatgca gaaattgcct
    46561 tcataatact gttaaccgcg aataatactt cgtcatcctg tgcatctggc tgcgcaataa
    46621 gatttgtaaa ctgtgcatga gtcttattgg ggctgtcacg agttacgcga ccaatgattt
    46681 gaatcacttc agttaatgag ttacgataac cgattgtcag tgcatattcc gcaaatggcc
    46741 aatcgaagcc ttcttttgcc attccaagtg caatgatgat atcaagctta tcaagatctt
    46801 taatgtcatc cgtaatactt gctagatatg ctgagacttt ttcacgtcct tcttcattca
    46861 caaggtcggc cactaatagt tttttaccat ctggacgttc gatttcataa ataccatcct
    46921 cgttctgtgt gatattacca ccgtccgtca ataaatcaag tatttcatca acttccgcgt
    46981 atttatcttt agtagactca ccagaattaa cattaggaat atggataatt gattttttgt
    47041 tgacatcaat tactttatcc aaagcatctg tatattttcc acgatagaaa ctatacccca
    47101 ttgcaaagga tttcagatat ttatatcctt ccagttgctc ataataagtg taagtgacct
    47161 tatcaaattt atcttcgtcg gctggttcca gaatctgaac agagtcgcca cggaaatatg
    47221 aaccagtcat tgctaagata tgcgcattag agtttgacat gatgctacgt aaggcattac
    47281 ctaacacaga tttatcatca cggctcacat gatggaactc atcaatcgct aatagcgtat
    47341 tgtcaaataa tgaatcgtcc aactcttcaa aagcaaatcg caacgtagca tgtgtggcaa
    47401 tcaggatatt atcacttggg tcagtcgagt tcatgaattt aacaaagtgt tgaacctttg
    47461 atttttcacc gcccccaata gttaagttgt cacttggctt tacttcccaa tcccaataaa
    47521 aaccaaactt cgataattca gtatctttaa atgatttacc aattgagcgt tcaggaactg
    47581 caataaccac ttttttaatt ccttgattat ggagtttatc caagccaaca aacatcaatg
    47641 cacgtgattt tcctgaagct ggtggtgatt ttaccaacaa atactgtgac gcacgctttt
    47701 cataaacacg ttcttgcatt tcacgcatac cgaggtcatt tgttgttttt gattgatttt
    47761 tatcaaaatt aatttcaaat aaattactag tcattgattg tcatctcctg atagagtttt
    47821 agtaatgttc caagtcgatc ttcgtcagat tcaaacgggc gttgctgata agctctatca
    47881 acaatcccat ccagttcctg gtgtttctta cgtaatgatt ctggcatact gtctttatta
    47941 tacaaatcgg ctaacgtgcc gccttcgtat tctcgtaaat caagaatttc tgtcatcacg
    48001 cgagtcattt catttttacg ctgggcggat aaatcctgaa tcggaaaagt attatagact
    48061 aacccggctg aataacggta ccgagtttct aatttcccac cgacagagcg cagccacacc
    48121 atatgcattc gggattctaa cagaccaagc agccacattg gagcgttata gattgccata
    48181 gccgaatctg aaattatagt atcgctccca actataccca taggaacata atctcttttt
    48241 tctgatgaca cacgaggaat tagaatacta ttctcactgg tttcatcata acgaacttca
    48301 ccaaatgacc atggcttctc agctagtgct cgagtttctt ttctcttgct tttttcccta
    48361 taacttttta cattttttat tgtttcttta ataagagaat tttgtttgta tctagttgca
    48421 tcttcctcgg tcaaccataa agtatatctt aagtctccat ttattaatcc tgttgatcct
    48481 aaatatttct taaataaatg tttaatatct ggatattttt gaatcaaatt ccagtattct
    48541 gccactgaaa aaactagatt accaccatca tttgccatac tgccaaattt caaagagggt
    48601 aaatttgaaa tactatttct tgattcgttg acaaatacat tttcaccatt agctaaatat
    48661 gcattaatat ttgaaacact cattactgac ccattctcta aatataactt aggatgaata
    48721 ttcacattat ttgtcatacc aataatgaca acagtcacac cggcattatt ctttgcattg
    48781 ttgttccact taaagctctg atatgcaaat gaaattctta cctcatcttt taaaaggtac
    48841 ggccaaagga tactaacttg ttcaccttga caaattgaat tagtagtaac aaatgcgaac
    48901 gagccatgag atgttgcaat atattgttta gctttccaaa accacccaaa aatataatca
    48961 agcaatttat attttcgaat atcgccaaca agtttttcca tatcttcacg ttgttcagca
    49021 ttttgaagac ttttgcccag atacggtgga tttcccatta tatatatttc atgattttct
    49081 ttgagtcgca acacctcatt ccaatcaatt cgaagagaat tggcacacac tatattccca
    49141 gcatctttta acggtaacaa ctcagcaatg gtacctggta ttgcttcttc aagctcttta
    49201 ttcatttgat gttcagcaat ccataatgaa agtcttgcta cttcatgcgc aaaatcatcc
    49261 aattcaatcc cattaaaatt accaagatga atcactgata gtggcatgga atcattattt
    49321 tgtaagtcgc ccaacaattg caaaatctta atttcaagtc tacggatttc cttataagca
    49381 atgatcagaa aattaccaga accactggcg ggatccaaaa acttaatatt agaaattcgc
    49441 gtcaacaaag cacttagatc attgatgatt gttctcttat tatcgtttct tgttttttca
    49501 gtaatgtcct tcagctcgtt ttcttcactg cgaagcttta aagtttcaaa tgactctgtc
    49561 aaatcatcta agaataacgg cttgatgact ttcatgatat tcggcacgct tgtataatgc
    49621 ataccggcaa cgtgacggtc ttctgctgaa gcaactgatt gaatcattgc gccaaggata
    49681 tcaggattga tttcattcca attcaataat tcccctgctt caataagcag ctctcttgaa
    49741 acttttgaaa aggtcaaatc cacatgtggc tcaccaaaca gttttccatt aacatatgga
    49801 aattcaagta gccactcatc cttgccattg cgagctaact cattaatatc caagattgca
    49861 aagagttctt tgattacttc gttaaaattc gaaccatctt cagccgttct tgtttttaga
    49921 acatttgtaa aggaaccctt attcataatt cctgtatctt ctgcaaacaa caagaacagc
    49981 actcgaatca agaacaagtt aaatgcatgc tcgttggcat caggattatc tttagccacc
    50041 acatcataca attttgcaaa acgttcagca gcttttcggt cagctggatt ttcagcttga
    50101 taatcagcct tctcaatgcc attccacgct aagaaaaagt cagcattagc cggtagctct
    50161 tcaattggaa tattcaaagt ttcatgagtt ttcgtatcaa ttgcagcaat atctaaataa
    50221 tcgtttacca agatatatcg tggtttagat ttttgctctt tgattgattg agcaatcgca
    50281 tcaatcgcaa tgacaacgtt gccttcaatt tcttcataat gaagtttgtt cttaatcaca
    50341 aatggttcac cattatcgaa ttttgatttt gcacgtgtaa tcgacgtttt ggggatatcg
    50401 tacaaggaca aaaattgagt gatgaaatca tcgcccctat ttgaaagtat tttttttaca
    50461 ccatcttcaa tttcagtgat gtttaaaact ttattttttg ccatttattt aacatcctcc
    50521 gcatctataa catctgaact aattatttca ctaacatcaa catttaaggc agcagcaatt
    50581 ttcaataatg actccattgc aaccaactca tttttaccga gttttgctat tacattggga
    50641 ctgacgccag atatttcacg aagttgagtt ttgttcatat tcaaatcaat caataacttc
    50701 cacaatttat tataagaaat tgacatacta cacctctatt ctactggtat cttcaattat
    50761 atcatctatg agctatcatt attatacgaa acatgaaata ttttcgcgaa cgcaaactat
    50821 ttttctaaaa taaaagcctt cagcatagat ttactaaagg cgagataaca aatattaatt
    50881 ccactttatt ttaaccggaa tccccaattg acgagctagt ttatcaattt tgattctgac
    50941 attctttttg ttgaaatttg cataaaccca tactccatca gctaccctgg ctgaatttgg
    51001 tcttccgtta tacatggatg gtttacgtcc taaaattata gcgaacgacg gcgtctcatc
    51061 gacaattaat tcttgcagtt gattgttgta gtttccatcc gcaaatagct gagaaacaaa
    51121 atcaaccaat aaacctctag cagatttcga agatatcttt ttcccatcat actcaatttc
    51181 atattgtcca gatgaactaa attcataaat ttgggtttca ctgtcttcgg aagtaccaaa
    51241 caaatcgata tttgcaattt catctataat ttcaaacact gtgtcatata gctgatccga
    51301 ttttgctttt tgaagtttat caatatacga agtattacca gcagttgaat tcatcatttc
    51361 atagtcagat ttttcttgga aatcttggat aagtcgtttt tcaagataat ctgtatctgc
    51421 ttttgacatt ttcccatctg ctcttgtaaa gaataaaact gattcaaccc aagctttata
    51481 ttccttgaga aagtgttgag ataaacgaag tgaaattgat tgccctgctg cctgacccac
    51541 gtaacgctta ttaccaccaa tcaaaacata aacagctgga taatttgaaa aatcctgttc
    51601 cttcaaatac ggcaaatcac tacgactcat tgaaaccaag attgctggct cgcgttcaaa
    51661 ataagaaata ttatctttct gtgtatctga aaatataaaa atttgcttca taagtttgtc
    51721 tcccttgaaa actcgataat atattatatc acgtaaccgt tatcaaaatc atctaaccta
    51781 tcaagtctac ggtgtaactc ctatcaactc aaagctccat agcatttcca tcaactctac
    51841 gctcacccaa aaatcgtcat ttcaacacga aaaaagagca atccggaaac catcccgaat
    51901 cgctctaatc cttgtcatat caacgttcca cacgtgtaag cagtcctaca cactcgatgt
    51961 gatgggtttg cgggaaaaga tctacaggct gaactttctt cacggcataa cctgcttctg
    52021 caaataactt agcatctctt gcaaaagttg ccggattaca tgagatatag acgattcgtt
    52081 ctggtgacat ttcgattgct gattcaataa aatgatgatc taatcctttt cgtggagggt
    52141 caactacgat cacagatggt ttgatccctt tttcttgcca ttttttcata actttttctg
    52201 cttttccgac ttcataatga acattttcga ttttattatt taaagcattg aactgtgcat
    52261 cttcaattgc ttctggaata acttccatcc catacacctt atccactctg tctgctaaag
    52321 acaagccaat cgtacctatt cctgaataag cgtcgatcac aatgtctttt ttatctaact
    52381 cggcaaactc gatggctgtt tggtacagat actctgcttg ttgagtattt acttgataaa
    52441 atgactttgc tgagattcga taggtttttc ctaatagctg atcttcgata tagtctctgc
    52501 catataaaac tttttctttg tctcctaaaa tcacatttgt tttttcttca ttgatatttt
    52561 ggatgaccga aaccacttct ggcaattctt catgaatagc ttcagcaatt tccttggatt
    52621 taaagaattt ttctttacga gtaactaaaa cgaccatcat ttcatgggaa taatgaccac
    52681 gacggataat gatgtgacga agctgtcctt catttttttc ttcattatat cctcgtactt
    52741 ggaaccgttg caagatatct ctgactactt tgattgcttg atcgatgaca ggatcttgga
    52801 tataaaaatc ttcgataggg actagatcat ggctattctt tttgtaaaaa ccggtggcta
    52861 actgtccatc gatacggcga acagggatct gtgctttatt acggtagccg aatggttctg
    52921 ccattccgac cgttggaagt acaggaactt ctggcatctt agcaatcttc gccattacat
    52981 gctctacttg tttttgtttg aagacaagct gttgttcata cttcaaatgg gctaaaggtg
    53041 cgatacctat gcgaagtaat gcattgtttt ctacaggctg acgatcaggt gattgtgtaa
    53101 taaatgtttc gacttttcca tatccaaact tattgcctgc ttttactact agtatttcta
    53161 cttcttctcc agggagtgca ttttcgataa ataatggata accatcgatt tttgctaccc
    53221 ccattccttc ataactcaag tcgacgattt cgactgtata tcgttcgttt ttctttacta
    53281 tttgctgttc tgtcatattt tgctccttta agcctatttt tcaatcctac tgaactctac
    53341 tataaatcag ctaattacac aactaaaacc ggcactgacc ttactacgtt cagcaccggc
    53401 ttttgattaa ttcttttgat taggtgtttc gtctgctatt tttccgtcac cgtcgatatc
    53461 ttcatccgtt aaacgttcga cttcttttac gaattctttg ctgacaacct ctaattcttc
    53521 ttcatctgta cctgtgattg cttcatcagg aatctgatcg gtattggcaa agaactcaat
    53581 gtgttgatga agattttgga aatgcatcgg tgcttccccg ccgtattcac catctaagtt
    53641 gatcattact ggtcgattga tatcatcttc tgtttcaacg aacaaactgc ttgttttggt
    53701 atagattatt cttggatcat cgacatgtcg accgccattc agcatgagtg ctgcaatatg
    53761 caagatctca aagatatttg ctgtcttcac aatgattaaa gaaaacttcc catcatccaa
    53821 tttggcatct ggcgcgatct gttcgaatcc tccaacagag ttcgtcagcc ctaggaaaaa
    53881 catcgaagca tttccgatat attccccttc atcatacttc aagcgcattt tgattggctt
    53941 tactcgaggg agcatctctg ctcctttcgc aagataagca agataaccaa aaatactctt
    54001 tagttctgat ggaacttcat aagtcaattc tgtcaaatag ccacctgcag cgatattgat
    54061 gaagtaactg ttatcagaaa ggccgatgtc catttttacg gtttggtgtt tttttatcac
    54121 ttctgctgct gctttaatat tatctctagg aatttttaat gctcgagcat agtcgttcgt
    54181 tgtaccagct gggatgatgg ccattttagg acggtgttca agcggagcga ttccattaac
    54241 cacttcgttg attgtgccat cgccaccagc tgccactact agatcaaaac ctagttctgc
    54301 gactctacat gcttcattct tggctgaatc tggttctggt gtagttgcat aggcgctggc
    54361 ttcaaatcct gcttcttcta atgcctgtag gatatccgcc aaattttttt tcaataattc
    54421 ttttcccgag gtggggttat aaattacgcg tgcttttttc attttcagcc tctatcgttt
    54481 cgctaattca tctttaagta gcttattcac gacaccagga tttgcttgtc ctttagttgc
    54541 tttcatgatt tgaccgacta ggaagccaac cgcacgatct tttccatttt tgaaatcatc
    54601 gacagattgt tggttgttat ccaaaatttc attaatgatt ggcagtagtt tgttcggatc
    54661 agataattgc acccaaccat ttttttcaac tacttcttgg gcttctccgc catttgtgat
    54721 caattcacgg aagacttttt tggcgatttt tgagctgatc gttccatcgg cgatcaaacg
    54781 aatcattcct gctaagttct caggtgtcaa ttttgtctca gctaattcca gtttctcact
    54841 gttcaagtaa gcagaaactt cacccatcaa ccagttagat gcttgcttgg catctgcacc
    54901 attttccaaa acagcttcaa aaaaatcaga catttctttt gtaagtgtca ataccattgc
    54961 gtcatactct ggaagtccaa actcacggat atagcgttcg cgacgagaag ctggcatctc
    55021 aggcaaacta gcttttactt tttgaatcca ttcgtcatcg atcacgaatt tcgggatatc
    55081 aggctctggg aagtaacgat aatcactaga cccttctttg acacgcatca aaactgtttt
    55141 attcgttgtt tcgtcaaaac ggcgtgtttc ttgttggatt tctcctcctg aaagaagaac
    55201 ttttgcttga cgtttttctt caaaagctaa accttttttc acgaaagata aagagttcaa
    55261 gtttttcaat tctgctttcg taccgaactc ttcttgtcca taagggcgaa gagaaatatt
    55321 ggcatcacag cgcatagagc cttcttccat tttcacatcg ctgacttctg taaattggat
    55381 aattgatcgc aatgcttcta gataagcgta tgcttcttct ggtgaacgca tatcagcttc
    55441 agaaacgatt tcgatcaaag gcgtgccttg tcggttcaaa tcaacgtaag aataaccgcc
    55501 aataccatgc atgttttttc cagcatcttc ttctaaatgg acacgttcga tccggatttt
    55561 tttcttttta ccttctactt cgatttcgat ccatccgtca tgaccgattg gttgatcaaa
    55621 ttgggaaatt tgatatgctt ttggattatc tgggtaaaag taatttttcc ggtcaaaatg
    55681 agtatcttta gagatttcac agtttaatgc caaagctgct ttcatcccga attccagcgc
    55741 agctttattc attactggca gcacgcctgg ataaccccag tcgatcacgt ttgtattgct
    55801 gtttggttct gcaccaaaat gagctggcgc tggagagaaa atttttgagt tggtttttaa
    55861 ctcgacgtgg acttctagtc caatgactgt ttcaaaattc attcgtcttt ccctcctaaa
    55921 atagctggtt gttgtttatg gaattctgtt gcttgttcaa acgcatatgc cgcttgatac
    55981 atcgtttttt catcaaaata attaccgatg atctgcaatc caactggtaa tccttctgat
    56041 aaacctgccg gtacagacat acctggaagt ccagccaagt tgacagggat cgttaagatg
    56101 tcactcatgt acattgtgat tggatcgtta atgttctcac cgattccgaa tgcaactgtt
    56161 ggtgaagatg gcccgatgat caagtcataa tctgcaaaga ctttttcaaa atctcgtttg
    56221 atcaatgtac ggacttgtcc cgcttttttg aagtaagcgt cataataacc agcacttaag
    56281 gagaatgtac ctaacatgat acggcgtttt acttcttcac cgaatccttc tgaacgtgag
    56341 ttgacatata catcatctaa tgatttcaca ttttctgagc gataaccgta gcggatacca
    56401 tcgaatcgtt gcaagttgga acttgcttct gatgaggcaa tgatataata aacagccaca
    56461 ccgtattttg aatgaggcaa gctgacttct tccacaacag cacctaatga acggaatgtt
    56521 tctgctgctt tcaatacagc ttcttttacg ccaggggcca caccttcgcc taagtattct
    56581 ttaggtaaag caatcttcat tcctttgatg tcgcctgtca gtccctctgc aaaatcagga
    56641 actgaaacgc ctgaagaagt accgtctttt tcgtcataac cgctgatagc tgtcaatgct
    56701 aatgcattat ctttcaccgt acgggtcatt ggtccgattt ggtcaagaga tgaagcaaag
    56761 gcaatcaaac caaaacgtga gacacggcca taagtaggtt tcatccctac gatgccgtta
    56821 aaagctgctg gttggcggat acttccccca gtatcactac ctaaagaaac tggaatttgt
    56881 ccagctgcaa ctgctgctgc agaaccacca gaagagccac caggtacttt tgttgggtcc
    56941 caagcatttt ttgttttctt gaaataagaa gtttctgtac ttccacccat agcaaattca
    57001 tccatgttta gcttaccaac agggatcata tctgcttgat agattttatc catgaccgtt
    57061 gcatcataaa ttggttcaaa attgtaaagg atcttagatg cagcggttgt caagatatct
    57121 tttgttacga tattatcctt gataccaatt ggaataccag caaggacgtt gctttcatca
    57181 atcccttttt catctaaagc ttttgcctgt tccaatgctt tttcttcgtt caatgtaatg
    57241 aatgcttcga cctcaggctc tgtttctttg attcgttcaa atgttgcttt tgtcaaatct
    57301 tgtacagtga tttctttcga tacaagcata tgatgcagtt cttctagaga acgattgtgt
    57361 aattctgtca ttatgcgcca gcctccccat tatcgatgat tgcgggtact ttgataaagc
    57421 cgtcttctgt ttccggcaca tttttcaata attcatcgcg gttccatcca gcttgcgccc
    57481 gatcttgtcg catcacgttt actgtttcaa ccacattcga cgtaaatgga acaccttctg
    57541 tatctacttc ttccagcatt tcaaccatat cgatgatttt acctaattgg tcggtaaaac
    57601 ttgcaagttc ttcatctgcg aacgcaagtt ttgctaattt ggcaacgtgt ttcacttgtt
    57661 cttcactaat cgccatacta ccccttcttt ctaaacgagt taaaaacttt gctctctatt
    57721 ttaacacaag agtgccgtgt ctgaaattgt ttattgaaat ttgtctgaaa aaactcattt
    57781 ttcattcttc tactgagcga cttttagcca atgcatctgc catttgatct tctgtccaaa
    57841 cttcgattcc taattcttgt gctttggtca gcttgctgcc agcatcttct cctgctacca
    57901 cgatatccgt ttttttcgat acgctaccag tcactttccc gccaagattt tcgatcgtct
    57961 cttttgcttc ttcacgagta aatcgtgtta gtttaccagt aaggactact gttttttctt
    58021 taaaaggaga ctcgacttct tgcaattggg catttcgaag ccctttgtat tcgaaattga
    58081 cgcctgcttg ttttaattca ttcatcaatt catggacttc ttcgttactg aaataagtga
    58141 ctacactgtc ggcaatgata tcaccgatcg tatcaagcgc aataatcgac tcatagtcac
    58201 ttttactcaa tgtttctaga tcaccgaaat gttctgctaa aatttttgct gctttcgctc
    58261 cgacatgtcg gattcctaac ccgaaaatca accgctcgac cgaattatct ttgctgttgt
    58321 cgattgctgt caaaatatta tttgctgatt tttcttttat tttgtccaac gtcaataact
    58381 cttcttctgt caatttgtaa agatcagcaa catctgctac tagtttttta tcatacatct
    58441 gttctagtac gcgcggcccc aacccatcga tattcatcgc atttcttgaa acaaagtggt
    58501 tcaaaccttc tttcatttga gcagggcatt taggattgat gcaccgtaag gctacttctt
    58561 catccaaatg gacaagttca cttccacaaa ctggacaatg tgtaggcaat tggtattctt
    58621 cgctgtcttt aggtcgttta tctaacacga cttgcgaaac ttctgggatg atgtctcccg
    58681 ctttataaat cagaactgta tctttcaagc ggatatcctt caacttaata taatcaccgt
    58741 tatgcaggct ggcacggctt actgtcgttc ctgctactcg gactggtgtc atgatagctg
    58801 ttggcgttac tacccctgtt cgaccgactg tccattcgat gttttcgatt agcgtttcaa
    58861 cttcttctgg cggaaacttg taagctgctg cccatctcgg tgctttgaca gtaaaaccaa
    58921 gttgatcttg tagcgagaat tcattcactt tgatgacgat tccatcgatt tcatagggca
    58981 aatccacacg tttgtcatga tattcttcaa tgtatgacca cacttcatca atcgaatgac
    59041 atagacgttt ttcccgattc gtgtggaatc caattttttc tagctcttct agcgcatcat
    59101 actgggtttt tgcttgcata ggaccgaaat cagctactgt gtataaaaaa gtgtccagat
    59161 ttcttttcgc tgtgatttta gaatctaact gacgcaaaga accagctgcg gcgttccgag
    59221 gattcgcaaa gatatctttt ccctctgctt ctctgtcttg gttcaattgg acaaatgacc
    59281 gttttggcat gaaacattcg cctcttactt cgatgttcat tggttctttt aatttgattg
    59341 ggaccgaccg gacagttttt aggttttcag tgatgttttc tcctacagtt ccatccccgc
    59401 gagtcgcacc tctcacgaaa tttccatctt cataacgcaa agaaacggaa agtccatcta
    59461 tttttagttc acagcaatag tcaactacac gtcccactgc tttttgtaca cgttgatcaa
    59521 aagctatcag ctcttctttg ctgaaaacgt cgttcaaact gtaaagcgga atatcatgag
    59581 tgactttttc aaaaccttct aatacttttc cgcctactcg ttgagtggga gaatcagacg
    59641 tgatcaaatc aggatattgt tcctcaattg ctactaattc tgcgtattct ttatcataaa
    59701 catagtcttc cactgttggt ttatcttcta cgtagtattc gcgagaccat tggttcagtc
    59761 ttgtacgaag ttcttctgct cgtttagcag cttcgtcaat cgtcatttca tgttccatca
    59821 tttaccctcc actactaagc tttttcaatt ggcgcaaagg cagccaaaag acgttttact
    59881 ccttgacttg ggaaagccac atctagttct agatccttcg acgtcccgct tactcggacg
    59941 actgttccta gaccccattt tttgtgtttg actttatctc caggtttcca gctgttattt
    60001 tcccctccag tagcactttt agagggaaca accgctgtct ctggctgttt atagctatat
    60061 gctgtttttt tagcgaatac aggtgttttt ttaggcgtag gacgcatgcc ttcgatttcc
    60121 agtaactcct gatcgatttc ttctacaaaa cgagaaggtc ggttatattg cgtccgtcca
    60181 tataatgttc tggagaaagc atttgttaaa tacaacgctt cttctgctcg cgtgatcccc
    60241 acatatgcga gacggcgttc ttcttccagt tcgctttctt ccatcaatgc tcgagataaa
    60301 ggaaaaaccc cctcttctag accaatcagg aatacaactg ggaattctaa tcctttggct
    60361 gcatgcaaag tcatcaatgt cacttgggaa gcatcttctt ctaaattgtc aatatcggaa
    60421 acaagtgcca gatcatttaa gaagaccgtt agtttttctt ctggtgcatc agcgtcttct
    60481 tcattttgct gttcgaattg tttgtcgaat tcttgtgtga ccgttaaaaa ttcctccaag
    60541 ttttcgatgc gtgcttgtgc ttctaatgta ttttggattt ttaagtcttc tagatatccg
    60601 ctacgatcca aaacctcttt cgtcaattct gtcactgtca agtatgggat catctgagtg
    60661 acttcttgga tcatctctcc aaatgcacct aattgctgtc ctgcttttcc accgatattt
    60721 gccagatcca cattttgcgc tgcttcaagc agtggccatt catgaagaga agcaaaactg
    60781 cgtagttttt cgatagatcc tggtccgatg cctcgtttgg gcgaattaac gatacgctca
    60841 aaactgattg aatcttgagg atttgccagc acattcaaat acgctaaaat atctttgatt
    60901 tctttacgat cgtagaactt gtgccctcca accattttat aaggaatatt tgctttcaac
    60961 agagtttctt ccattacacg agattgtgcg ttcgtccggt acaagatggc aaagtcgccg
    61021 taattccgat gattgcttcg aatctcttct tgcatccgat ctacgataaa acgggtttcg
    61081 tctcgttcat tatctgcacg gtagtaagtg atcttgtttc cttctttgtt ttctgtccac
    61141 aagttttttg gttttcgatt gctattgttt tcaatgactt ggtttgctgc gctaaggatg
    61201 tttttcgtgg agcgatagtt ttgttctagc aaaattactg cagcatctgg ataatctttt
    61261 tcaaaatcca agatgttctg catatctgca ccgcgccagc cgtaaatact ttgatctgca
    61321 tctcccacga cgcacaggtt cctaaatcta cctgccaata aattgactag cgtatactgt
    61381 gcgtgatttg tatcttggta ctcatctaca tgaatataat ggaatttgtt ttgataatat
    61441 gtgagtgaat caggatgttc ttcaaataat cgaatcgtat tcatgatcag gtcgtcaaag
    61501 tccatacatt gattatattg aagctctttt tggtaagcag cataacattt tgccgcaatc
    61561 tcttcgtaat aactgccttg catttttgca aaatcctgag gggtctgcag gctgtttttt
    61621 gcttgagaga ttgttcctaa tatacttcga ggatcatatt ttttcggatc aatgttcagt
    61681 tcttttagta tccgtttcat caaggtcagc tgttcagaag aatcgatgat tgtaaagttc
    61741 cggttatacc cgataaaatc aacatctctt cttaaaatac gcacacacat cgagtgaaaa
    61801 gtcgataccc agacatcttc gcctccagat gcgagaatcg cattgacacg ttctttcatt
    61861 tcttttgcag ccttattggt aaaagtaatc gctaggatgt tccaaggatt gacttctttt
    61921 tcctcaatca aataggcaat gcggtgtgtc agcactctcg tctttccact tcctgcacct
    61981 gccattagca gtaatggtcc gtcagtgtga agaacagctt ctttctgacg ggggttcatc
    62041 ccgtttaata attcagcttt ggatgacatt aacaacatcc tttcttttat caatcttgtt
    62101 aattatagca ttttcttggc tttcattaaa ctagctagtt caaaatcatt tgtttctttt
    62161 tatggatttt tccttgtcaa ttcgttgatt tcgcgctaaa ttaaaagaaa gcgaggcgtt
    62221 tgaatggaaa aaaaaggaaa aatcaagaaa ttagataaga ctcagatata ttttattcta
    62281 aaaggaatat ttgttggact gctgacagga atcgtcgtta gcctgtttcg attatctatc
    62341 gaaaagctaa gcgatttagt tcgaagtatc tatgaaatga gtcgggaaca gccgatttac
    62401 ttaattggcc tagctatttg ctgcatcctt gcagcatttt tcgtgggtta tctggtcaaa
    62461 aatgaaccgg atatcaaggg aagcgggatt ccacaagtag agggacagct tcgcggggaa
    62521 ttatcaatga actggttctc tgttttatgg aaaaagttca ttggcggtgt actttcagtt
    62581 ggtgcgggcc tttttctagg tagagaagga ccttctatac agcttggcgc atccattgga
    62641 caaggagctg gacaattgtt tcggagtcca tcttcagaag aaaagatttt aatttctagc
    62701 ggtgcaagta gcggacttgc agcagcattc aatgctccta tcgcaggatt actctttgtt
    62761 ttagaagaaa tccatcactc cttttctcca cttgtttggc tgacatcatt tgcttcagca
    62821 atcactgcta acttcgtctc tctttacttt ttcggcttga gacctgtttt atatctaggt
    62881 gaccttcctt ctttgccgtt gaaatattat ggttccttag tgatcttagg agtacttcta
    62941 ggcatcttag gttttattta tcagaaggtc ttgctggctt tgccaaaatt ttacaaccga
    63001 ttgcctttac cagcttattt tcacgggttc gttcctttct tgctgatttt gccaattggt
    63061 tatttatggc ctagtactct aggcggaggc aatcaaatcg ttttagcttt tggacatgct
    63121 tccttgccaa tcattatgat tatcggtttg tttgttcttc gattcgtttt ttccatgatt
    63181 tcttacggtg gtggtcttcc aggggggatt ttccttccga tattgacatt gggggcttta
    63241 atcggtagct tttatggaaa cattatggtt gatttcggga tggatccaat ctacatcaag
    63301 gattttgtcg ttattgccat ggctggttat ttcacagcga ttggaaaagc accgctgaca
    63361 gcaattattt tagtgacaga aatggttggc tctcttaatc atctgatgcc tttaggattg
    63421 gctgctcttg ttgcttatat cgtcaatgac cttctcggcg gtaatccaat ttacgaatca
    63481 ttattggaac gtctacttgg aaatactcag accgcctcac taaaaggtgc aaggacaact
    63541 tttgattttc cagtaacggc agagagttct ttagacggat taatggtacg agacttcaat
    63601 tggcctaaaa atatgctgct aatctctata cgcagaggaa atcaggaaat cttgactcac
    63661 ggtgatacga tcatgaatgt aggagatatt ttggttatct tgactgatga agcaaacttg
    63721 ccaagaatca agcaggaaat cgaggctaaa tcttctatca caaatttgaa gcaatcattt
    63781 taacgaacat ggaaaaacta ttttacaaca actattcatc tacactactt gattctatat
    63841 gattctataa aaaaagaaga gactgggaaa aaccagcctc ttctttttta tttctatgct
    63901 tctgctgctg caggagcttt tctcaataaa ccataaatca caccagatac gactgctccg
    63961 atagcaataa acaccaaata catgaccggg tgactcagta agaatacaac gaaaatcccg
    64021 ccatgaggag ccagtaattt gatcccgaaa gctccgacta atccgccagt taaagcagaa
    64081 ccaataataa aacttggaat ggcacgaatc gggtcagctg ctgcaaatgg aatcgatcct
    64141 tccgtgacaa acgatagtcc catcacgatg ttcgtcaaac cagcatcttg atctgttttg
    64201 ctgaatttat tcttaaacaa acgtgtagca acgaaaattg ccaatggagg taccattcct
    64261 ccagccataa cagaagccat cacgacactt ccgccagttg caacagtcgt agctaaagtt
    64321 gctgtaccaa acacataagc ggctttgttg attggaccgc ctaaatctgc cgccatcatt
    64381 ccagctaata atgcaccaag taaagcagca ttcgaaccac tcatacttgt caagaaaccg
    64441 ttcaatgcat cattgattgc tttcataggg acattgacca gcaacatcaa gaaaccagta
    64501 atcagtaagc caaacactgg ataaaagagg atcgttttaa ttccatccaa tgattttggt
    64561 aagttcttga ataatttacg taaaaatacg atgacataac cagctaagaa tccaccaatc
    64621 agtgcaccta agaatcctgc accgcctgta ttagctaatg caccagcagc aaatccaacg
    64681 atcaaaccag ggcggtcacc gatacttgaa gcaataaatc ctgctaatac cggcagcatg
    64741 aaaccaaatg cagcttgtcc gatttggttg aaccagctag cagcttgatt gtaattccct
    64801 agatttgata attgatcatg tggcacgcca ataaattggt cgatcatgaa agaaagcgca
    64861 atagcgattc ctccaccaat aacgaaaggt aacatgtgtg atacgccgtt catcaaatct
    64921 ttgtaaattc tttgtccgat agtaccatcc gcatttcctt cttctgaact gctttctttt
    64981 ccatcaccat ggaatactgg tgcagtacca ctaagggcta gattgatcag ctcttctggt
    65041 ttacggatac catcactgac aggacgattg atcagttctt ttccgtcaaa gcgattcatc
    65101 tctacttttt tgtctgctgc aacgattaca ccttctgcac gggcgatgtc ttcagcagtc
    65161 aatcggtttt tgattccttc tgaaccgttc gtttcgactt tgatctccac acccatttct
    65221 ttcgcttttt tcttcagagc atcttctgcc atgtaagtgt gagcgatccc tgttggacaa
    65281 gctgtaacgg caacaacgaa ttttctttct ggatgagcag aagcttctgc agcttgtgtt
    65341 tcttgttctg ctttttcttc tgcttctttt tgctcttcag ctgtttggaa caagtcttgg
    65401 acttcttctg gtgtatttgc ttcttttaat tttcctacga aatctgcatc aatcagcaat
    65461 cgagatagtg ctgctaatgc ttgcaaatgt gtgtcgtttg cgccttctgg agctgcgatc
    65521 atgaaaaaca gataagttgg ctgtccgtct aaggcttcat aatctactcc tttttggctt
    65581 tttgcgaata aaactgttgc ttctttaact gcactgtttt tcgcgtgagg catggcgatt
    65641 ccatcaccaa gacctgtaga agtttgtgct tcgcgggcta gaattccttc tttatatgtt
    65701 tcgatatcgg aaatccggcc ggcatcaaac attttttgga ccatttcatc gattgcgcct
    65761 tttttatcag tcgcttgcaa atccatgatc atcgcttctt tgaccattaa atccttgata
    65821 ttcatgatta tcctccttga aactaatcaa tttttgtgat ttcaacttct ggcagcaact
    65881 catcaatcaa ttcgcgagct gccaagtcat cagaaaaagc tgttgcgctt ccgcaagcaa
    65941 ctccccattt aaaggcttcg attggatctt tcgtacggct gtaagcccca ataaagccgg
    66001 caatcattga atcccctgca cctactgaat ttttcaatgg gcgaactaat acattcgttt
    66061 tataagttcc ttgttccgtg aagagcaaag caccgtctcc agccattgag atcagcacat
    66121 tctgtgcccc ttcagctagc aattttttac catacggaat gatttcttct acagattgga
    66181 aggttgtatt gtaaagctca gctaattcgt gattgtttgg tttgatcaac aatggtttct
    66241 taacaagtgc attttttagg tcatcacctg ttgtatcaat cacgaaatct gcaccttttt
    66301 ctcgaatgat atcaatcagt tcttgataaa atccttttcg caggctagcc ggcgcgcttc
    66361 ctgacaatac gacgatatct ccttgtccta ctgttcctaa tgcttgcttc aactcatcga
    66421 tttcttcatc tgaaatagat gggcccaaac cgttaatttc agtttcagta tctgatttta
    66481 gtttgatatt gattcttgta tcttctttta cggttgtgaa caaagactgg atttgttctt
    66541 tttcaagcca cttcgaaata aattcgccag tgaaccctcc taaaaaccct agtgctgtag
    66601 attgagaatc gatccgtttc aaaatacggg aaacattgat cccttttcct ccaggtaact
    66661 tgaagtcttc cttcattcgg ttcaaatcgc ctagttttaa cccatctacg cgtacgatat
    66721 aatcaattga tggattaagt gttactgtgt aaatcattgt attgcctcct taattgttgt
    66781 cttttcacgg aattcattaa cgaattctaa tggacaacgc tcggttatga tcgtggctgc
    66841 ttccaaaggt gcaacagagg tgaaggacaa ctggttgaat ttggtatgat caatcaatac
    66901 aaatgtctct tcggaatttt tcatggctaa acgtttaaca gctgcctctt ctgggtccgg
    66961 agtcgtgaat ccttgttcaa gatgggcacc gttcattccc ataaatgctt tattaaaatg
    67021 aaaatttttt agttgttcta tcgcattaga tcctaaaaca gcattagttg ccagctttat
    67081 cgttccacct aaaataatgg tagcaagctc caaatcaact aaatttgccg catgttgaac
    67141 ggaatttgtc actactgtga tctgttttcc ttgcagaaaa ggaatcattt ctaacgtagt
    67201 agagccagcg tctaaataaa tgacatcttc gtcattaatg gtttcagctg ccgatttggc
    67261 gatttgctgt ttttcgtgaa cgtttttgag tgatttttca ctcatattct gctcaaaatt
    67321 caataattgc tctttcttag ctccgccatg aactctttct agaaacccac tattctccag
    67381 ctcttgtaaa tcacgtctta tcgttgattc agaagcatta aaaaggttta caagatcttg
    67441 tgatttcacg attttctgct gttcaagcaa gtctaggatt ttttgtctac gttcctctgt
    67501 aagcattttc acgatcacct caatacaaat aatagcatat tttttttcta ctcgcaatat
    67561 aaatcagtca aaaacgttca taaaaattat aaatcagtca aaaaaaaata aaaaaccagt
    67621 caaaaagact ggttttttat ttttttgtct atcatcaagc cgttttatcg ttttttattt
    67681 tcgcttcttg tttttttcat ataaacagaa gcaggttttc tttccttatg aagaagataa
    67741 taataaagtc ccatcccgcc aaacatgacc aaaaaaccga tgactgatcg aataggaaaa
    67801 ttaggaacaa aaaagagaca gacggataat aatgatgccg gaaccattat tcgataatag
    67861 agttgtttat ccattttctt cacctcatat tttttgtaaa gtaatcaatt gttctaattc
    67921 tcggtaatct aaaaattgca cacgcagatg ctgctcacct ttcttgacat cgaattggat
    67981 acttcgccgc tttttcttta ttcgtttgaa ggtaacatca gagagtctat gtttgatttc
    68041 ctttaacggt aattctgaca gggattctga aggaacatag attcttaagg ataaaagata
    68101 ctcttgatat ttttcatctg ttacctctac ttctagatat tccctaatat aataacaata
    68161 agcttcgatt ccattatcca aagcaacagt cggatcgata actttatagc acatcatgcg
    68221 cgagcgattc tcgtcaaccc aaggaaaata cgcctgagca tttagtgttc cgcgaacacg
    68281 cctttcttta taatctcctt gttgaatatc ataacttttt gttacaaaaa atgaagcatt
    68341 ctttttagga acaggttgca tttgtgcatt ctgatactgg acagattgga tcgtatggtc
    68401 agtgtaaatg tattcaacga atggcggttt caataaaccg acaccattgt cccaagcaag
    68461 atgatcaaac ccaagctggt ccctaaaata ggattgaaac atttgattgt ttggcttttg
    68521 ccagttaaag gggtctcgat aaagctggat acatatgact tcgttttcta attccttcac
    68581 tttttctact tgttggatgt cgagaaaaat cggtttaggg atgatttgat gataatagcg
    68641 actataatac atttcccaac acgaagtgaa acataaacct tggtaaaaaa gatcgtatcc
    68701 tggaaattgg ttgcaatcga ctactacttc cccgttttgt tgtttcattt taggtaaatg
    68761 ttcgatttca tcctttttct caaacaatag ccgttgagag atctgttttg tattgtgata
    68821 gataaactcg ctataagctc gcatataggc gaacacccca tgagcaatca tttttttctg
    68881 aatgaagtct cgaatcaaaa attgcttttc tttgaaaaca gagatagaca atacattttt
    68941 tatcaataat tgattatctt ccaagcgaaa actacattcg ttctcgtcat ttcctacaac
    69001 taaccaactt tcgtctctta ccgatgctag cattattttt tcttgaagct ttcgaagatc
    69061 atacggatcc gcttgttgtg tgaaagagcg gataaatgtt ggctggatca acagacgtgt
    69121 caaaatagcc caatcgctta tttcctgttt tttttcttct tcatttaagt ctgtgagctg
    69181 gatagtcatc aataatttat cttcgccaat cattttctca cctctttaaa atctttttat
    69241 aatttaatta taaccaacga taaagaaaaa ctaaaacttt atgtcttaga aaccaatttg
    69301 gacgattaca tgatagacta agtaaaaaga aacaaagagg tgtttacaat ggggttgaaa
    69361 tttgaacgta cagataattt agataaactt ttcgaatcat ttgctgtcga tccggaaaaa
    69421 acagttgcac caaaagaaga gatcgatcga tttctaaaac cacagccaca aaatgaaaag
    69481 aaaaaagaca agaaacaaaa gtaagttaag ctcaaaaaaa gacttaccag ccaaactttt
    69541 atgaaagagt ttgactggta agtctttttt attcttttta tgattcaatt gcttgttcga
    69601 ttgctgccca cgcttcttct tttccctgtt ttgtaacgga agaaaagaga ataaacgtgt
    69661 cattgcgatc aaaatccaac gcttttttga tttcgctctc atgcttgttc catttaccac
    69721 gagggatctt atctgcctta gtggcaacaa caattacagg gatatcataa tacttcaaaa
    69781 attgatacat ctggatatct tcttgagaag gcttatgacg caaatctact aaagaaacga
    69841 ccgcacgtag ctggtctcta gtcgttaaat atgtctcgat catctttccc cattttgctc
    69901 gctcagtttt ggatactttt gcatacccat atcccggaac atcgacaaaa tgtaatgcgt
    69961 tctcgatcaa ataaaaattc aatgtctggg tctttcctgg cttaccggaa gtacgggcta
    70021 aattctttcg atcaatcaat gtgttgataa aagaagattt ccctacattt gatcgtcctg
    70081 ccagagctat ttctggcaat tgtgtatctg gatattgttt tggtgccact gcacttatga
    70141 taatttcagc ttgatggact ttcatggtct ctctccttaa caaggtgatg aataaagcaa
    70201 gcggctgtcg caaaagccaa aatgactttt acggcagcct cttttcatca acccgctttt
    70261 ttctctgaat ctttatagat tactgttggc tcaccagaca attcagctgc ttcttttgtg
    70321 ataatcactt tttcgatgtt ttcatttgaa gggatatcaa acatcacatc catcatgatt
    70381 tcttcaatga tggaacgaag tcctcgagca ccggtattgc gttcgattgc ttttttcgca
    70441 atggctctca acgcttcagg ttcgaattca agttcggtat tatctagaga aagaagtttt
    70501 tgatattgtt tgactagcgc atttttcggc tcagtcaaga tccgtaccag atcatctgtt
    70561 gtcaatttct ctaaagctgc cataacaggt aatcgtccga taaactcagg gatcaatccg
    70621 aattttagta aatcttcagg aatgatatgc tgcatgacac tttcgtcttc agaaagtttt
    70681 tggttttgtg taccaaatcc aatcgtcttt tcgcccatac gattcttcac aatcgtttcg
    70741 atgccatcaa atgcaccacc tacgatgaac aatatgttcg tcgtatcaat ctgaataaat
    70801 tcttgatgtg gatgtttacg tcctccctga ggtggcacgc ttgcaacagt tccttctaga
    70861 attttcagca aggcttgttg gacaccttcg ccggaaacgt cacgagtaat agatacattt
    70921 tcgcttttac gcgcaatttt atcgatctca tcaatataga tgatcccttt ttctgcacgt
    70981 tccacattgt aatcagcaga ttgaagcaat ttaagcaaaa tgttttctac atcttccccc
    71041 acataaccag cttctgtcaa acttgtcgca tctgcgattg caaaaggtac attcaatgtt
    71101 tttgctaatg tttgtgctaa aaatgtttta ccagaccctg taggtccgat caagcagata
    71161 ttgctttttt gcagttcaac atcatctgtg ttttcttgtg cttgctgatt gacacgttta
    71221 tagtgattgt atacggcaac agctaacgag cgttttgcgc gatcttgtcc aatcacgtag
    71281 ttattcaata cttcaagaat ttctagtggt ttagggactt cagtgaattc gcgtacagct
    71341 tcttcataaa attcttcatc aatgatttct ttacataggt cgatacattc attacagatg
    71401 tagacccctg gaccagctac gatctttttc acttcttctt gggttttccc gcaaaaagaa
    71461 cagcgtacgg tctcattgct acttggattg tcatacatgg tcttcacccc ttaacttgca
    71521 aaaagtatgc aagaaactat caaagacttt acagtctttt actatcataa catagtttag
    71581 taaaaagaaa aagtgtttaa caaaggatca actgacctat acctttgcat tttttactct
    71641 taacaaaaaa taaggaactg tgacaaaatc gtgtcacagt cccttatttc gaataaacaa
    71701 ttttctagaa gtagcttctt cagaaatgat ttattacttg atttctattc tttcgcttct
    71761 gtcgctgtac ctgtgatcaa ttcgattgct tttttcattg tcacatcatg ttccagcata
    71821 tcttctgtca acacgcgttt gatttgttcg attggcatgt tgtatgtttc tgataattct
    71881 tggatttctg cattgatttc ttcttctgtt gcttgaagat tttcagcagc tgcaattgct
    71941 tcgatcacaa gatttgttct tgtgcgcatt tctgcttcac cttcgaattg tttatgcaag
    72001 tcttcttctg tagaaccagt taattgatag tacatttcag gagagatacc ttgacgttgc
    72061 atgttgttca agaattcgtc cattgaacga tgtacttcat catgaaccat cacgtgtggc
    72121 aattcaacga tttctgcgtt ttcaacagcc atacggatcg ctgcttcatc ttttgcatcg
    72181 tccgctgctt tttcttttgt ttctactaat tctttgcgat atttttcttt caattcgtct
    72241 aatgttgata cttcgtcatc aacatctttt gcgaattcgt catctaattc aggcacttct
    72301 tttgctttta cttcatggat tgtcactttg aagactgctt cttttccagc aagatcttct
    72361 gcttgataat cttctgggaa agttacattt acatctagac tttcacctga tttatgacca
    72421 actaattgat cttcaaaacc tggaatgaat gagcctgaac ctaattcaag tgagtaattt
    72481 tcgccttttc cgccatcaaa cgcttcgcca tctacaaagc cttcgaagtc gattactact
    72541 gtatcgccat tttcagctgc agcatcttct ttgataacta attcagcttg tgcttcttgt
    72601 tcacgtttga tacgtgcatc cacgtcagca tctgttactt cacggtcttg tttttcaaca
    72661 actagatctt tatattgtcc taattttact tctggtttca ctgttacttc agcagtgatt
    72721 acccagtctt gacctttttc catgctttca acatcgattt ttggttgaga aacaggatcg
    72781 atgccagctt ctttgacagc agcttcataa gcttctggta atacagcatt caatgcatct
    72841 tcatataaag cttcttcacc gtacatacgg ttgaatactt gacgagaaac tttcccttta
    72901 cggaatccag gtacgttcaa gctttttttc actttattaa acgcagttgt taatcctttt
    72961 tggatttcgt cttgcgcaat cgtaaatttt aaaacaccat cattcgtgcc ttttttttca
    73021 aatgttgcag tcatttaatt ccctccgagt ttagaaattc attttataca tcaaaaacgt
    73081 ttcatgcata cctttaaata gtacactaac cctttcaaat tgtaaacata ttgaatggat
    73141 agaatccttg tttttcataa catttttatc tttttcactg ataaaatcta atggaagaag
    73201 atcatttgct gcaaaatttt ttctcttttt tttaaaaaat cttgtttttc atcttctgta
    73261 ccttcataca tcgtttttcc tgtacacgcc tctacagtca gttgaaacca gcgcttcggg
    73321 tcttcgatgt atgtatcata gacaggataa acaattgcag attgcaacgt gaattcttca
    73381 tttaacacgg aactaagcag cgcatcttca ttaccgtaac gatctgaaaa tacacataat
    73441 tgctggtaac ttgcctgctg ctgcggcttt ggcagatctt ttggtatgac tttgactaac
    73501 ttttcttcaa tcgtcaaata gaaaacagga tggcattcgt tcaattgcct aagtgtctcc
    73561 agtaattttg cccttgctaa aatatggacc tctggcgata caataagttt actcgctgtt
    73621 tggataaact catgataagg aagctgttcg atctcatcaa tagacgcaag ctgacgtgca
    73681 ggttccaacg aaggaagttc ctccgattta ttgtggagtg ccttgatctt ctgcctctcc
    73741 atttgtccgg ataattcttc tgcaaattgg attttttcaa tgacccgctg ctcgaaagct
    73801 tcataaaaat ctgtacttgc tatcaatttt ctagcatata aaaaatcttg tgtactgatt
    73861 aacaagtcaa aataaaattc agcaaactcc tcattttgaa gatactgttt ttcatgcaag
    73921 agtgcttgct tcaatgcttc ttttttctcg tccaactcgt aaagacaaaa aacaagcagg
    73981 ctattagcct caaagctttc tttcaactcg taagctcgtt gaaataattc ttttgcctgc
    74041 tgatattgct ggtttgctag tgcttgttgt gctactcgca ttaaattctc atatttttct
    74101 ggaaaatcaa tcggcttcac gcagtgtcct cctcttgctc atgttcagct catgttcaat
    74161 agtagcaaaa caaaaaaagc atgacaatta aaaataactg aagcaccctc taaaagctag
    74221 attttagatc tgacttttag aaggtgcttt caactgttac tgctttttat tgctcatcag
    74281 ctgttgactt cctggttgaa tgctttgatc agattgatcg taacaacact tgtattagtg
    74341 aaaaaatcgc ctagatgttg ctttttctga ttaaagattg ctggaagata ccctaacatc
    74401 agcaaaggaa attttagaat gaaccggcaa gcaccttctc taactaaaac ggttgaccag
    74461 ctaagttctt cttcatcaaa gctgatgact cgaataccaa agatcatttt cccaatcgtt
    74521 tgaccatgat tgaatttcgt caataaaata aaatacgcta gataaataag taaggctgtg
    74581 aatccgtaga tactcagata agaatctgaa aaattaagcc aacctagatt tttggcaata
    74641 ccaagtgtta aacgcgtaat agctccgata cacaaaagat cgaccaagaa agcaaacatt
    74701 cggataaaaa aacctgcata gaaatactta ggaaaatcat ttgcttttgg cggttcattt
    74761 cttgtgtccg tatacttttt ccatttacgt tgtttctttt tgatttcttc gggcgtcaaa
    74821 ggctctcttt caaaagagct cagtaccttt tgggaaaagt tggagtcagg tcctacagca
    74881 ggctgctgtt gcttatcttt ttcttgttcc ttattttcat ttgaaagctc tgacatgctt
    74941 actcacctcc gtaataatac attgcttttg gtgattccgc tgtccctaga ttctcaagaa
    75001 cggataagat ctgactagtc tcagaaggct tcaatccctg gaattctgct agtttacttc
    75061 ctaaccatgt actagcaaac ccagatggcg tggaagaata ttctactagt tccgcatctt
    75121 caagctgttg ttcctttcgc atagctttta aagcatcttc aggaaatccc aattcatcga
    75181 ctaaacctac ttcttttgct tggacaccat cataaatcct tccatcagca atttttttga
    75241 cttcttcttc agatttattc cgtccctcgc tgacgattgc tacaaaacga ctatatgcgc
    75301 tgtcgatata tgcttgaagc accgcttctt cttcttttgt ctctggtcgc gttcctgaac
    75361 ccatatcttt caacgctccg cttttaactg ttgtatcttc cacacccagt ttttctagaa
    75421 gtcctgaata attcaatcct gacatgatca caccaattga gccggtcact gtttcttctg
    75481 ttgcaaagat tttatctgct tgagcagaaa tatagtagcc accgctagct gccatgtttt
    75541 tcatgctgac atacattggg atatcatgtt ctttacgaat cgtatccaat ttcttagcga
    75601 tttccgcact ttcgtagaca ccgccaccag gagaatttac ttctaaaaag atacctttga
    75661 ctgttggatc ttcttcaatc gccttcagtt gttccataaa atttttatga ttgtaacctt
    75721 cagaagcaaa taaacctgaa gaccctcccg cagcaatcgt tccatctact gataatttta
    75781 cgattttttg cgtactgtct ccctcttcca acacatttga agctagttct tcatccccat
    75841 ataaccaact gttcaatccc tcaatttgat cgttttcctg aggcttagtc aaactagctg
    75901 aaactaacga aaccacaaat aatcctgcag caatcagaac tgctgcccaa cgtctcttat
    75961 tcattctttc tcctttctaa tacaagtttt ttatgacgat cacagtttct gactgctcag
    76021 aagtgtcgaa ccgctctgct ttttcttccc gcgcataccc ggaaaacgac tcttttgtca
    76081 attcatcttc taccgtaaac tcacgagctt cagcaacttc caaggcatca ggtgcgattt
    76141 cttggatgct atcccagcca gtatctaaaa atattttcgt atcatctgat acaccagctg
    76201 atgcttcgaa tttggacaat tgttttttta acattcccag tgtcatgatc tcaatttttg
    76261 aatccatttt tactcctcct ctatccgatt actttctatt gtaccgaact cctgaaaaag
    76321 atgatagata aaaacccatt attttttgac ataaaaagag catttgacaa agcatttgat
    76381 atataccaat ttatttttta ttcttttttt gttgacaaga acattcgcaa aaggttatga
    76441 ttttaaatgt ttccagataa aaaacaaaag aggaggatag ttataccttt taggatagcg
    76501 ccaggtctgt gtaaacagat gacgaggaga gggtttatcg aaagattcgg cggatggccc
    76561 tagggactgc actctacaga aaatccacaa aaaatatctg caaagataaa acaaaaggat
    76621 tttcctgcag ctggaagcat tcatacgaat tcattataga aagtaggatg ttttttatgt
    76681 gcggaattgt tggaatgatt ggattaaaaa atgtaacacc aggattgatt gacggtctag
    76741 aaaaattaga ataccgtggt tatgattcag ctggtatctt tgtctcagat ggcacaactg
    76801 attatctggt caaagcacaa ggcagaattc aaaatttgaa aaacaaaata aatattgata
    76861 ctacaggaaa tataggtatt ggtcatacac gctgggcaac tcacgggcaa ccttctgaag
    76921 aaaacgccca tccacatact tcccaaagtg gacgcttcgt gctcgttcac aatggcgtaa
    76981 tcgaaaactt tgaagaactt aaaattgctt atttagcaaa tgaccacttt attggtgaga
    77041 ccgatactga gattatcgct catctaattg aaactttcgc gaaggatact acaacgcaag
    77101 aggcgttttt aaaagctttg cgggtcatca aaggttctta cgctttcgct ttgattgatc
    77161 ggacagcacc tgatgttatt tacgtcgcta aaaataaaag tccactatta atcggactcg
    77221 gcgatggatt caatgtgatt gccagcgatg caatggctat gcttgctcat acgaaggaat
    77281 tcgttgagat tgaagatgaa gagatggtta ccgtcacatc agaaaaagtt attattcaaa
    77341 attttgcagg tgacatcgta gaacgttctt cattcgaagc acaagtagat tcttcagata
    77401 tcgagaaagg tacctatcct ttttatatgc tgaaagaaat cgacgaacag cctattatca
    77461 tgcgtcgtat cgctcaaaaa tacgtaacag aagacaatcg tgtctctctt gatcaaaagc
    77521 tggtcgatac attgtctgat agcgatcgaa tctatatcgt tgcttgcgga actagttacc
    77581 atgcggggtt agcaggtaaa cagactctgg aaaaattgac acagatccct gttgaggttc
    77641 atttagccag tgaatttggc tataacacac ctcttctttc aaaaaaacca ttctttattt
    77701 tcttgagtca aagtggcgaa acagcagata gccgccaagt tctggtaaaa atcaaccgtc
    77761 tcggctaccc ttcacttacg attacaaatg ttgctggttc tactctttcg cgggaagcat
    77821 cctttacact gctattgcat gctggacccg aaatcgcagt agcatcaacg aaagcttata
    77881 ctgcacaaat agctgtacta actttattag ccaaagctat cggtgataaa aaagaatttg
    77941 aaacttctct tgtattcgat atcactcatg aattgagttt agttgctaat gcgatggaat
    78001 cggtcatcgc ccaaaaagat tatttagaag agttggcaga agtatatctc agcgatacaa
    78061 gaaatgcctt ttacattgga cgtggcgctg actacaacgt ttctcttgaa gctgctttga
    78121 aattgaaaga aatcagttat atccaggcag aaggttttgc cgctggcgaa ttgaaacatg
    78181 gaacgattgc tttgatcgaa gaaggaacac cagtgattgg aatcatcagt gaagaagtga
    78241 ctggtgcgca tactcgagga aacctgaaag aagtcgaaag ccgtggcgct aaaacactag
    78301 ttattgtctc agagggatta gaaaaggaaa gtgaccagct ggtgctacca actgtccacc
    78361 cttatctaac tactctaacc acagtcgtac cgactcaatt acttgcatat tacgcaacgc
    78421 tattacgggg attcgacgtt gacaaaccac gtaatttagc gaaatcagtg acagtagaat
    78481 aataaaagaa caaggaccag tgtcagacat ctgacactgg tccttgttct tttatttttt
    78541 atgaaaatat ctcttaatcc agtccgattt cttctttgac tactgccgcg attttatcga
    78601 catagtaatt aactttctca tcagtaggcg cttctgccat cactcgaagt aatggttctg
    78661 taccggaagg acgaactagg attcttcctt ctgatcccat ttcagcttct gcctcttcaa
    78721 tcactttttt gattgccgga acatccatcg caccgttttt atcacttact cggatattca
    78781 ctaatttttg cggatagatt gttacctctg ctgcaagctc tgataatttc ttacctgtct
    78841 gtttcatgat attcagtaac tgaattcctg aaagcatccc atctccagtt gtgttaaaat
    78901 ctaagaaaat catgtgccca gactgctcgc cgccaaagtt ataatcgttt ttgcgcattt
    78961 cttctacaac gtaacgatca ccaacttgtg taacgacatc tttcaaaccg attccttcca
    79021 ccgctttgtg gaatcctaag ttactcatga cagtcgtaac gatcgtatct tttttcaatc
    79081 gatttttttc tgccaagtat ctagcacaga taaacatgat tttatcaccg tcgacaatgt
    79141 ttccaagttc atccaccgca ataatacgat cgccatcgcc atcgaaagct agtccagcat
    79201 ctgctccttt ttcaacgacc atctcagcta aacgttctgg atgggtagag cctacgccat
    79261 cattgatatt taacccgtct ggatttgtgc ccatggtata aaaatctgtt tcaagatcgg
    79321 caaacaaacg attaacactt gttgaagttg ctccgtttgc tgcatcgaca caaatggtca
    79381 aaccggaaag gtcaccgttg atcgtttgta caaggaattg agaatatttc aataagcctt
    79441 ctggaaattc ttctacagtt cccaatcctt cagctgatgg acgaggcaat tcatccactt
    79501 cagcatctaa taatgcttca atttctagct cttggtcatc tactagttta aaaccatctg
    79561 caccgaaaaa tttgattcca ttatcttgtg ctggattatg agaagcagaa atcatgaccc
    79621 ctgcgcttgc tttttgcaaa cgtgtcagat aagcaacacc tggtgtagag atcacaccta
    79681 gctggaacac ttcgataccc accgataaaa gtcctgaaat cagcgcttct tctaacattt
    79741 gacctgagat tcttgtatcc cgccctacta acacgcgtgg gcgttttcct tcttcttcat
    79801 gctggcttag gacataacct ccgcaccgtc ccagcttaaa tgcaagttct ggcgtaagtt
    79861 ccttgttggc ttcaccgcgt acgccgtctg ttccaaaata tttacccata gttgtaaaat
    79921 tactccttta acttaatttt ctgaggaatt tgttctgctc atttgtgttg attcgcttgt
    79981 ttctgatgtt tcttctgtac ttgtggtcga gctgcttgtc ccagattccg aactggaaga
    80041 aatggttgtt gtcgatggtt ccgttcctga atcagcagat gaaatacttc tactcgtact
    80101 tgtattttct tgtgtccgac tgctcgtcga agcagagcta ctgcttgacg tttgcaacac
    80161 gggtgtaatc tccacactaa ctgattcggg cttgatactt aacccctctt ctactggtat
    80221 ttcaatttta cgagttgttc gatgattgat acctgagaca ttgatcggta cagcaatact
    80281 gtcaccgatc gatgcaagca aatcgctaga gccgcttact tctgcttcca gttttgaaaa
    80341 actgaaatca tagctttcta ccccggcagc tggtgttcct tgctgtatcc cataaagtcg
    80401 taccgacttg cttggcttga tcgaatcagc tctcactgtt acttgcactg gatcagagac
    80461 gatacttaat acttcccctg ctgcattcag cgcctgtatt gggacctcac tattaatccc
    80521 atcatctgtg accatcgaag cgtccacatc agctactact cgtttgattt ctgccaatgt
    80581 tttgtcacct gtagtgatag agactttttt aggactaacg gaagtatcat caagttcata
    80641 tccagatggt gtgaacgttg aaggaatcaa cgtttccaca tcgaaatctt ttattacctt
    80701 tttctcaacc gttactgtaa tagtcgatgg ttcgatcttt gctgtgacag cactgctcaa
    80761 attttgaatt tttagcggaa cctcatgtgt tccttcgccc agcttagtga tatctgctgt
    80821 gacgcggaac atccgtgtat cttcgtttgc ctctgcattc aactgaactc ggtttgcact
    80881 ggaaagtttg acagtcactg cattttcata tccatggata tagtagcgct ttgaatcata
    80941 agaaatcttg attggcaccc cagtaactgt ttgattatac gcttcatttc cagaaagcgt
    81001 atttggaacg gactggccat ttgcattgaa gtacagcgca atactgaaca gcaatgcaat
    81061 aaaactgtaa agaatgcttg tccgtttctc ttttgagatc atttgcgatc ccctccctta
    81121 gtcataccgt ctaggaaaga ttgaatcacg tttttcttgt tttttgtttt ctcttcagca
    81181 atcagttctc tacgcaaaat tgccaaatat tcttgctggg ataagccagc catcagttca
    81241 ttatctaatg taatgctgac atccccagtt tcttcagaga caacgatggt aatcgcgtcg
    81301 ctgacttcgc ttataccgac agctgcccgg tgtcgagtcc cgaattcttt tggaatcaac
    81361 atactttctg atagaggaag ataggcgctt gctactgcga tctttccttc ttttacaata
    81421 actgcaccgt catgcagcgg tgtattagga ataaagatat tgatcagcag ctctccagtg
    81481 atatctgcat ctaatgcaat tcctgtctca atatactcat ctaatccagt atgccgttcg
    81541 atagtaatta gcgcaccgat ttttcgcttg gacatatatt ggatggcttt gtcaaaagac
    81601 aagatcatct tctcatcttc ctgctgttcg ctttttgatt gtttgaaaag cgaactgcgt
    81661 ccaagatgtt ccagtcctct ccggatttcc ggctgaaaaa tcacgatagc agctatcacc
    81721 ccgtacgtaa tgacttggtt catcagccaa gacaaggtgt gcagaccaat gatttccgct
    81781 acgattcgga taacaataaa aatagctaca cctttcagaa gctgaatcgc ttttgtgcct
    81841 cgcagcagtt ggatcagttt gtatacaaga taccagacaa ccagaatatc aagtatgttc
    81901 accaaaaaat tcagagagaa caagtcaccg gaaaacattt gctgccaata gtccaaatta
    81961 aaaagttccc cgatttgaaa tgacataaaa agcctctctt ctcttttgag atttcctctc
    82021 tagtatatca tatgttttaa ttttagaggc tgatctgctc gtatttttct tgagaaaaac
    82081 gcataatttt caggaaaatt gcaagtatgc gttttatttt tctctgcaat aaatattttt
    82141 aaacttcaga gaattattca ttatattttt tctgttattt ttcctttttt tcttttgtag
    82201 cttgttccat ttttttacgt cgttctgctc tggcagctct tctttcttct gcttcaaggt
    82261 ccgtcgtttt ttcttttcta gtcttaacgg gtacatcgat agattcctct ggttcaagtc
    82321 gtttcttgat tttttctaat tctcccgtca gtgcagcata gttgtaaaaa cgtgttttag
    82381 cgtcttggat agtcttttca aataaagctt ttgcaagatt tggatttgcg ttctctagcg
    82441 aggaaaagcg aacttgtttc ctcatgaaat caggcatagc agagaaatca ggttttttgt
    82501 agtccaaagt cattgggttc ttgttttttt cgaccattgc agggttatat cgatataatg
    82561 accaatagcc agaagaaaca gcttcttttg cttctttaag cgattctttc attccaccaa
    82621 gcaaaccatg tgtgatacaa ggcgtatagg caatgattaa agaaggtcca ggatactttt
    82681 ctgcttcttc aaaagccttt atcgtctgca tttgattggc acctgaagca atttgcgcca
    82741 cataaacatt tccatatgtc atcgccatca ttcctagatc tttctttgaa gcatactttc
    82801 cactagccga aaatttagcg attgccgaag ctggtgttgc ttttgaggtt tgaccgccag
    82861 tattggaata gacttcgtta tccaaaacaa gaatattcac gtctgctccg ctggcaagta
    82921 catgatcgat cccgccaaaa ccaatgtcgt aagcccatcc gtctccacca atcatccatt
    82981 ggctgttttt tacgaataga tcctttctct tgtatatatc ctccagttca gggacaattg
    83041 atcgttcgct ttcgatcgca gcttgcagtt ttgctgctct ttgctgcgtt ccttcgctct
    83101 caaacatgtg taccaaccag tcttccatca attctcgtag ttcttctgat acttttggca
    83161 atacttcttt gattgaactt gccaaagcct cgcgacgagt ctgtgccgct agaagcatac
    83221 cataaccaaa ttcagcatta tcttccaata aagaatttga ccatgcaggc ccttgaccac
    83281 attcattggt cgtataagga gaagcaggag aagcaccgcc ccaaatagaa gaacagcctg
    83341 tcgcattcgc gatcatcatt cgatcaccga acatttgtgt caataatttt acataaggtg
    83401 tttcgccaca gcctgagcaa gcgcccgaga attcaagaag cggttgattg aattgtgtag
    83461 acaagacagt attctttcct ttgactggat ttgctttttg tcttaatgtc attgcaaaag
    83521 cccaattcac agcttgttct ttctgttctt cataaggctt catcactaaa gctttttctt
    83581 ttgcgggaca agcctctaca cacagaccac aacctgtaca gtcttccact gaaacttgga
    83641 tacggtattt tacgccgtct gcgcctcgca tttctctcac gataaatcct tctggcgctt
    83701 cttccatttc ctcttcatct gctaaaaatg gacggatggc agcatgagga caaacaaagg
    83761 cacattcatt acacatcgta cagcgatcac taatccactc aggtacttct aaagcgacgc
    83821 cccgcttttc catagaagcc gttcctaaag gcatttcgcc agttgtcatt ccatttgcta
    83881 caagtgtacc aacagagagt tgatttcctt cctgtgcatt cactggttca agtatttctt
    83941 gtatataacc agtgttcctt ttattgattt tttcaggcat ctccaacgtt ctccaatggt
    84001 ctgggatcgc gacttgatga agcagttcgg tcgtccgatc gattgcttgt atgttttttt
    84061 ctacgatagc cattgattta tgtccatagc ttttcagggc ttcttctttt agcagcggaa
    84121 gcacctcatc aaaaggaatg atgtcagcta atttaaaaaa agccgtttcc attgccgtat
    84181 tgatccggcg tcccaaaccg acttcttggg ccaatctcat ggcattgatc gtatagaatc
    84241 taatgttatt ttcagccaag taacgtttta attttaaggg cagatgcgtt tctaattgtt
    84301 cgtcgctcca aagagtattc aataaaaaaa tgccacctgg cttcaatcct ttgaccaagt
    84361 catagctatg caggtaagct ggtgtgtgac aggcaacaag gtccgcatgc tcgacaagat
    84421 acgctgaacg tatcggcgta tctccaaaac gaagatgaga gacagtcaga ccgccagatt
    84481 ttttcgaatc ataataaaaa tagccttgcg catatttatc ggtgtgatct ccaataatct
    84541 tgatagctga tttatttgct ccaactgttc cgtcagatcc aaatccccaa aacttggcct
    84601 ggaatgtttg cggttctgtc aaatccagac tttcctttgg ttccaaagat tgataagtca
    84661 catcatccac gatccctatg gtaaaccgtt ttttgcgtat gcttgggtct tttttcaatt
    84721 catcaaaaac tgctgagatt tggtcgggag tcacatcttt agaccccaat ccgtagcgac
    84781 caccaataac agccgggcga agttcagaat catataacgc actttgaacg tctaataaga
    84841 gaggttctcc acctgcccct ggttctttac ttcgatcaag aacagcaatt gtttttactg
    84901 tatgaggcaa cttttgcacg aatgtttcca ctggaaaagg acgataaaga tggatattca
    84961 aaaaacctgt tttccgtccg tttgcattca agtaatcaat cgtttgttca atcgtctgtg
    85021 caactgatcc catagaaaca atgacttctt ccgcatcgtc tgctccgtaa taagtcacca
    85081 agtcataaga agttcctcgt aatcggttga tctctttcat atatttttga acgatttcag
    85141 gtaatttatc ataatagggg ttgatcgttt cccgttgttg aaaatggatg tctggattct
    85201 ggttcgtacc actaacagtc ggatgattgg gattcatccc tcgtttacga aatgaagcta
    85261 atttctcctg attgatcaga ggagctaatt cttcatattc caaaacttcg attttttgaa
    85321 tctcgtgact cgtacggaat ccatcaaaga aattcaaaaa aggtacttct ccttcaatag
    85381 aagcaagatg ggcaacaggt gctaaatcca tcacttcttg tacactgctt tcagctaaca
    85441 tagcaaatcc tgtttgtctt gcagccatca cgtctccgtg atctccgaaa atattcaacg
    85501 catttgtcgt cactgcccga ctagcaacat ggaaaacact tggcagtagt tccccagcaa
    85561 tcttgtacat attcggtatc atcaaaagca gcccttgaga agcggtatat gtagttgtca
    85621 gtgcaccagt tttcaatgat ccatggacca caccagctgc tcctgcttcg gattgcattt
    85681 ctactacttt tactggttga ccgaatacat tttcttttcc tgctgctgac cattgatcta
    85741 ctaattcagc catggtcgaa ctgggtgtta ttggatatat tgcagcaatc tcggtaaatg
    85801 cataagaaat atatgcggct gccgtattgc catccattgt tttcattttt cgcatgattc
    85861 atcatccttg ttcatattct ctcttattat ctgttccatt ataacgtaaa acattccatt
    85921 ttttctttta tttataaaaa tatttcacat atgtctattt gaattggtca tctaattttt
    85981 agacaaacag tctaaatcgt tgcctttttt tgtcagacat gtataaacta taagcgaaag
    86041 aggtgtttta acatggatat atcagtgatt gatgcaacga aaacaaatac acaaaaagga
    86101 atcctttatg gttcatcgaa tgctcctaaa aaaatggtcg aattcatcaa cctatcttgt
    86161 ccgtattgcc gacaatggtt tgaagaatct tatgagctat tagaagaagc agtccaaagc
    86221 gggcaattgc agcgagtgat caaattgttc gataaagaaa aagaaagctt gcagcgagga
    86281 aacgtgatgc accggtatct cacaatcagc gatggtcaaa aagcgatcaa agagatcaaa
    86341 caaatattcg atactcaaga cgagtggaaa catctttctc tccaagaagt agcggatttt
    86401 gcagtagata aactaaagct gacagaacaa aaagacgaac agctgagcca agctgtcatc
    86461 aacgaagctg agcaagcaca tattcgattt gttccgactg tcatcttggg taaagaaatc
    86521 tttgatgaat ctatttcaat agaggaatta aaagaattga tccaaacaaa ataaaaaaat
    86581 aaggagcgta ggacaaaagt aaagaacact tttgtctcac gctctaaaca cctataaacg
    86641 gcggaagcag aagcaactcc ttcttatttc ttggagttaa acgcttctgt cccagcctcc
    86701 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
    86761 caagctgagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
    86821 actaattctt aaattttcat acaaaatttc tcaaattccc ttaacgagta atatttatgt
    86881 acggtgattc acactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
    86941 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcgtttg ttgagctagg
    87001 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
    87061 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
    87121 actcctaact cctctcattt aatagttgct ctctttttta ttcagataca tttttgctga
    87181 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
    87241 actggctgat gaaatggatt aatagatttc gattattcaa agcgattttc tattggtgga
    87301 ttgtgttccc aactaacaag acggattcca aagtcattat ataactcttc aagagttaac
    87361 cctttcctgt tcccaataga tgtatctctc ccacgctcga tataaaatat ggagtttcga
    87421 atgccaagac ctggcaaatc tttccttgtt acagttacac tgctactaac ttccccatat
    87481 aaaatccagc tcctccaaaa tgataatata tttttcgacc atcatctaac tcatgtaaca
    87541 ataattgttg tgccctcttg tcactcgcca aatgcaaacg ccacgtgcga taccatgcca
    87601 tgctacctgc atctgcagaa aatccatcaa ataatgcaac aattgtatct tctatcacaa
    87661 tcctttcccc atctacttca tattctaatc taaaatcaaa ctcccctcgg gtaatcgcag
    87721 gtcttggtgg cttaggaccc aatattattc tacttaaaac cgcattcgtt cccagatgaa
    87781 acacagctat aacaacaata atgattaata ttttgtttct atttgataaa tttctcatta
    87841 ttcgcaaatc acctctacat tctcacatac tagttcgtaa tactccttta atatcaaaat
    87901 atctactgat cgtaataata ttcgctatga attcctacta ctcgtataag aaaggccaaa
    87961 tcatcagctt ttacttgctg atgatttggc ttgtttctat ttatcgtaat tgttttctaa
    88021 tcaccaaaat tgttaacaat cggttcagat atttcccaat tcactagcct tatattatat
    88081 tgatttaaca attcttcttc atttattgta ttttgatcaa gcggaccaaa ttgttctaat
    88141 aattctttgt catgtaaaat atcccttcta gtaatgcctc tttccctttc aatgaaagaa
    88201 gcactaggaa aagtatgatt ataaccattc aacctatctc ccatgtaata attagcaggt
    88261 cctggaaaat aaaatatttg cttattatca ctaacttcta acagcaacac taaatcttct
    88321 ccgctggcta aacgacttgt ccatctacga tatctcccta tgccttcgtt cattccaaat
    88381 ccatcaaatt caactattaa cgtgtcttct atcacatgta attcttcatt caattcatac
    88441 tcaagtctaa aaggaaattc tccatatgta atctctggca atggtggtgc tggagttctc
    88501 caaataaata caataactgt gaatagaaaa aatccaacga tcgatataaa tgttatgaga
    88561 cctgctcgaa gtaaaacccg atataataaa aatgctgtat ttcgctttgc tatatctaga
    88621 aaaataaaaa gtactgcaat cataatgata gtaaaagttg taaaaaaacg catttttgcc
    88681 ctccctttat aaacatcgat tctcaaattc taaaccaaat cttggtagtt caagttcagg
    88741 tataacgtat tccaaccata catctcaatt actatttatg aacttatgat tatcactgaa
    88801 taaatcactc catccccttg ttaaaacttc tacattaatt acacctggtt gttttcgact
    88861 agctaatcca atgaacttat caaccatcct tatccaccaa ccacgagaat cttgaccaga
    88921 agtaagaaga gcagttctca aggatgatcg agcagttcct tccagttttt taattaatca
    88981 ggccagttat agataaatac ttgatcttat ctatatcagg acttctatat taagcaaaaa
    89041 tttatgtaca aaactaaaat cttcattcag ttgtctcccc gctttttata cgattgtatt
    89101 aaaaacaata gaagcctttc caaattatgt tactccagta aagatggaat tatagtttga
    89161 cggaagaaaa gcaagtataa cttttaaaaa aaactgatac attcgcatct cctgcttcat
    89221 gtatcagttt tttctttgga ttaaactgct tcttcacgca gtttttccgc aaattcattt
    89281 attttgcgaa ttcggtgatt aattcccgat tttgatatcg cacctgaggg aatcatttcc
    89341 cctaattctt tcaagctgac ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt
    89401 ttttcaggaa gggcatgtag accgaccttt gcttcgatca attcgatgtt ttcgatttgt
    89461 ttagaagccg catcaatcgt cttgttcaga tttgctgtct cacaattgac tagacgattg
    89521 accgaatttc tcatatcccg tacgattcga acatcttcaa atcggagcat tgagttggtt
    89581 gcaccaatca atgttaagaa atcagcaatt ttttctgctc cttttaaata agaaatatag
    89641 ccattgcgcc gttcaagcgt tcgagcatgt agaccgtaat aatttaacat atcgcaaatg
    89701 tcttggttat gctgttcata aatagaaaat atttccaaat gataacggct cgtttccgga
    89761 ttattgactg aacctgaggc cataaaagca ccacgtaagt aagaacgcat tttttgcgaa
    89821 tttcccataa tctcgtccga aacgtgtcca ttgaacatca taccatccat gatatcaaga
    89881 tccatcaaca catcttttgt gccttgcttc aaccggacga tatagacatt gttctttttt
    89941 aatttcattt ttttacgaac gagtagctca cttcgtacct gataatgatc tttcaaaaga
    90001 gaatagatac gtctggcaat tgcagcattt tctgtttgga cgttcaaaat aaactgctgg
    90061 ttcacaatac ctaaagagcc gttcattcgc agcaaagcag ctaattccgc tttcgcatgt
    90121 tctcgatgaa cttccaaacc agttagttct ttcttcacat ctgcagcaaa tgacatccta
    90181 ttctcctcct ttctccaaca ataaattgct taatattttg aaccgaaaac taaacggaac
    90241 aattcttcta ctactttgtc tccatcgtgg aagacaccac catctttcaa ttccaaaaag
    90301 tcggtggaaa tcactcgaca tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt
    90361 actagatact catcatagat ctctgggtcc atataatctt caggcacttt ttccgtattg
    90421 accaatactg tatcgataaa aggctgattc aaatgcttgt gtaaaacacg tatatgatcg
    90481 gcatctgtga aatgttccgt ttcccctttt tgggtcatga tattacagat atagacggtt
    90541 tcagcagttg tcttgagcat tgcttcgccg atttctggta tcactaaatt tggaaggata
    90601 ctggtgaata aacttccggg tcccaaaacg accatgtcgg cgtccataat tgctgaaacg
    90661 acttttcgag ccgctttggc ttcactttta tcatttgtgt tgcagacata cacacggtca
    90721 atggttttac ggtcgatcgc gattttagat tctcctactg cagtcgttcc gtctttaaag
    90781 acagcatgca aggtcaaagg agtttctgaa gaaggataaa catgtccatc tacatgcatc
    90841 atttttgcta ataattgaat cgcctcataa gtgcttcccc gcatctcgga aactgcggca
    90901 atgatcagat taccgatcgt atgattggct aaaaactgat cttccttttt gaaacggtat
    90961 tggaaaatat cttcatagaa ttgtggcata tccgataacg ccactaagac atttctcaag
    91021 tcaccaggag gagccatagc gatcgattct ctgattgcac cgctgcttcc cccatcatct
    91081 gcaacagtca ctactgctgt gatatctgct ccttgatttc gcaaactttt caggataact
    91141 ggcagaccag tgccaccgcc gaccactacg atctttggtt ttcttatccg gtaggttttc
    91201 attttcacga ccggttcacc gtctctttcc ttttatcctt gtcccgatgt gtcacattga
    91261 ctttatagtt tttccctaaa gcatttgcca aacgttcagt caatgccaca gaacggtgtt
    91321 gaccgcctgt acagccgatt gcaactgtca agctgctttt tccttcttta atgtatccag
    91381 gcaatacagt ttccagaaga tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg
    91441 acatcacata atcatagact ggctgatctt ttccggtcaa tggtcttagt tccgggatat
    91501 aatgagggtt gggcaaaaaa cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt
    91561 atttaaaacc aaatgataca aattcgatcc gaaatccttg gtctcccttt gatttgaatg
    91621 actcattgat tttttcacgt aattgtcgtg gtgtcaatgt cgtcgtatca atgatcacgg
    91681 aagcttgggc tttcaagtcg tctagtatcg cccgttcttt acgtatccct tcagtgatca
    91741 aaccgtccat cgccattgga tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt
    91801 cgtctgaagc atctaaaaat aagatagaag tgtcgatgaa gttcgtattt tcgatttcga
    91861 tcagcatgtt ttggatttct tcaaagaagg aacgagaacg taaatccact cctaaagcca
    91921 tttttgtgac tttgcctgat tctttgatta attcccagaa ttttggaatc aatcgaggcg
    91981 gcatattatc gatacaaaaa tagcccatat cttcaaaact ttggatagct actgttttcc
    92041 ctgcaccgct catacctgtt atgatgacta attgcaaatt atctgtcata attacgaact
    92101 cctctcacat tgtgcatagt aaaattatat cattccgggc gtatcattac cagttttctt
    92161 tttcttttac aaaaagattt ggaccaaata tcttttagga tatttgcccc aaatccaatt
    92221 tttttacttt tccgatgctg tatccaatgc cttgcttctt tcagctagcg ctcgttcaat
    92281 cgtccgatct gctaaaatag attgggcatc gatcactgga taatggttat cttcagcaaa
    92341 ttcatgcatc aatgacaatt ctgtgcaacc taagataact ttttcacagt ttaggcgctc
    92401 taccgcttct tctaagattt cgtaatatag ctcttgattc aaataatccg attccttgat
    92461 ctcatgatag atcagataat tgattttttc ttgaagaaca gtatcaggaa tcactgtttc
    92521 gaatccaaga tttttgactt cgcgttcata gattccagct ttcatacttc cttctgttcc
    92581 tagaatcgct actctccctg tcgtatgctg acggacaagt tcatttgctg cttctcttgg
    92641 catatgaagg ataggaatat ccgttgccgc ttgtaattct tcaaaaaagt agtgtgcggt
    92701 gttacaagtc aacacaatga agttcggctg caataaattt tgtttctcaa tatcatccag
    92761 cagaaacggc attggatttt cttctgatcg atctaaaata taagctgtac gatcaggaac
    92821 tgttgcatga ttgaataaga cataattcaa atattcttga tcttttgtag ctttcgttcg
    92881 gtggttgatc aagcgaacaa aactttctgt cgccatcgtg cccattccgc ctaaaatact
    92941 gaaaaaattc tccatgaact agccttcttt cacatgaaaa tactttttga atcttggaat
    93001 atagttatgg aacatatatt tcatcaacaa ccagcgcttg attgaccggt ctttttcata
    93061 aaacacagta gtaccgtaac gattttcttt gatcatttgc tcagctagtt ttttgtcttc
    93121 attttcccga gcatattcta aaaagatttt tttgggcaca cccatccata atcttgcttt
    93181 atctgattga ttcgttccat aggtcgtttc aacaaagggt ttgttaaaca ctcgatcttc
    93241 tgtgacgaaa cgagccagat tcaagccgtt caatgtcaca aagaaactgc tacgaccttg
    93301 tctcaaattg atttcaaata atttatattc tccatctcta ggatcatact tcatatcgaa
    93361 attggcaaag cctgtatatt cgattttttc caaaaatgcc ttaatggttt gatagatttt
    93421 ttcgttataa tctggcatga tcacaacgta attaccaatc gatgctggtg taggatcttc
    93481 taacagtgga tgaccaagac acatcatccg aacttgatga tcttcatcta catacgcatt
    93541 tagcacacgc atattactat catcacccgg gataaaatct tgtacaatca tttcactttt
    93601 ataaccggct tcgtatatcc ggcctaaaat taaatcgaac tcttctctag tctctaaaat
    93661 aaaagctttt tttcttcctt caaattggac agaaagatac tccacactgt ttgcaggttt
    93721 caaagcaact gggaattcaa aaggcaattc ctgttccaaa tgtccattta ctaacatttc
    93781 ttctctgacg atcaatgttt taggatacgg aagatcatat tcttcacaaa cttcataaaa
    93841 gcttacttta ttgatcagtc tttcaaataa agaataatca atatatggac agataaaata
    93901 ttctgacagc tcttgtttat gttgggagat caattctgca tatccatcac cacaagcaat
    93961 caaaagatat tttttactct tatcagaata tctttctttt gccaaacgaa gcatcgtctc
    94021 gataaacaca gggtctttat caaatcctgg aataacttct acattgacaa ttttactgta
    94081 gcgggtaggg gctaactgat ccgaagcata tgcttggcag attttgccgt atgcttcatt
    94141 aaaagaacgt gccataccat aaacgttcat gtcgcttcct aataatatag ggataaattc
    94201 ttcattttca atactgttca tcattatctc tcacttactc ataatctctt gtttttcttt
    94261 tccaactaca caaggaatat cataccataa agaaaaaagc aatcaaagga aactcctccg
    94321 attgctcaat tcttttttga acggattgaa cttataggaa gtttttgtct ataccaatca
    94381 gtgtagaaag aactaccatg taatgattct agaacactga tattaataaa acaagcaaaa
    94441 attcattttt ttatgttttt ttaactatct cagctatcta tcctctttat accattattt
    94501 ttttcaaata gtgacctgta taactttctt ttacgttaat gatttcttct ggtgttccag
    94561 tcgcaaggat cgttcctcca ccttctccac cttctggtcc taaatcaatg atataatctg
    94621 ctgttttgat tacatctaga ttgtgttcaa tcactaaaac tgtgttacca gcatctacta
    94681 atctttgtaa tacatgcaac aagcgggcga tgtcatcgct atgaagtcct gtcgttggtt
    94741 catctagtat atagaaattc tttccattag agattttgtg caattcactg gcaagtttca
    94801 tccgttgtgc ctcaccaccg gacaatgtcg ttgctggttg ccccatcgtc acataaccta
    94861 agccaacatc aacaatcgtt tgcagtttgc gatgaatctt tggaatgtgc ttgaagaatt
    94921 ctacagcatc ttctactgtc atttccaaaa tatcagcaat gctttttcct ttgtaatgca
    94981 cttctaatgt ttcagagtta tatcgtttgc catgacatac ttcacaagga acatagacat
    95041 caggcaaaaa gtgcatttcg atcttaataa ttccatcccc gcgacaagct tcacaacgac
    95101 cgccttttac gttgaaacta aaacgccctt tcttataacc ccgcattttt gcctcgttcg
    95161 tttgagcaaa taacccacgg atatcatcaa atacacttgt ataagtcgct ggattacttc
    95221 tcggcgtccg gccgattggg ctttgatcga tatcgatgat cttttcgata ctttcgtagc
    95281 cggaaattgt cttgaattta cctggcttag cagaattctt atttaatttt tgcgctaacg
    95341 atttctttaa gatactattg accaatgtac tctttccaga accagacaca cctgtgactg
    95401 ctacaaattc tcctaatgga aattcaacac taatattttt cagattgttt tctgaagcac
    95461 cagtgatctt gattgctttc ccattacctt tacggcgttc ttttggtaca gggattgatt
    95521 ttttccctga aagatatttc cctgtcaaag aattttcatc attagctact tcttcaggag
    95581 tgcctgcagc cacgatttgt ccgccttgtt ctccggcccc aggtccgacg tcaatcagat
    95641 aatctgctgc ccacatcgta tcttcatcat gttcgactac aacgagggtg ttgccaagat
    95701 cacgcatttt ctttaatgag ccaatcagtc gatcgttgtc gcgttgatgc aatccgatcg
    95761 atggttcatc taaaatatac aaaactccag aaagattcga tccaatttgt gtagccaatc
    95821 tgattcgctg cgcttctccc ccagataatg ttccggaagc gcgactcaaa gttagatagt
    95881 ctagtcccac attttccaag aatgaaagtc tgtcatttac ttcttttaaa atcggtttgg
    95941 caattacttt ctcttgttca gataacgtta agtttgagaa gaaatctact gctttttgaa
    96001 tagacaactc atttgtttgc ccgatattcg ttccattgat ttgaacagat agagcttggg
    96061 gattcaatcg ataaccttta catgtctgac aagttaattc tgtcatataa agacgcattt
    96121 gctcacgcgt gaaatcactg ttcgtatcat gataacgacg ctcgatattc ttcaataccc
    96181 cttcgaaagt cgtttctaca tcgcgaacgc caccaaaatc attttcataa tggaaatgga
    96241 aaagatcatc tgtcccattc aagatgatat cctgatgctc ttccggtaat tcttcgaatg
    96301 gtgtatctaa atcgataccg aaactttcac aagcctgcgc taacatttga ggataatact
    96361 gagagctgat tggattccat ggaacgatag caccttgact aagtgtcttc gttctatctg
    96421 gaatcactaa atcttgatcg acttctaatt tgacacctaa accatcacag tctggacaag
    96481 ctccaaaagg cgcattgaat gagaacagtc ttggttctag ttcgccaaca gtaaatccgc
    96541 aataaggaca ggcatagtgt tcgctaaaca acatttcttc ttcaccgatc acgtcaacca
    96601 gagcatagcc gtctgccaat cgcagagccg cctctaaaga atcaaataga cgagaacgaa
    96661 tcccttcttt cacaatgatc cggtcgatga cgatcgcgat atcatgtttt ttgtttttct
    96721 ccagctcagg cacttcactt aaatcataga tctcaccatc gactcgaacg cggacgtatc
    96781 cctctttttg gattcgttcg aatatttttt tatgttgtcc ttttttcttt accacgatcg
    96841 gtgccaacac ttggatcttt gtgcgctcag gcaaagccat tacttgatct gccatttgat
    96901 ctactgattg actggtaatc tcgatatgat cgttgggaca aatcggatgt cctacacgtg
    96961 caaacaacaa gcgtaaatag tcattgattt ccgtcacagt acctactgtc gaccgaggat
    97021 ttttacttgt tgttttctga tcaatagaaa tcgccggact caatccgtcg atgctatcta
    97081 cgtccggctt atccatttgt cctaaaaact gacgagcata agcagaaagg ctctctacgt
    97141 atctccgctg cccttcagcg tacagcgtat caaatgctag ggaacttttt ccagaaccgg
    97201 acaaacctgt aacgaccacg aatttatctc gtggaatcgt tacatctata tttttcaagt
    97261 tatgggcacg tgcaccgtga atcgtaattt tatcgttagc catgttgtcc tcctactttg
    97321 aagcttttaa ttctaaaatc atatctcgca cattagcggc tgtttcaaaa tccaatgctt
    97381 tcgccgcatt tttcatttct ttttctaatt tcatcaataa cgtatctttt tcttctttcg
    97441 tcatttcttc atacgagact tgtaccgctt cttttgtatc atcttccgat tctttcgtaa
    97501 tagaaatcaa atctcggatt tctttaatga tcgtttttgg aacgatccca tgttcttcat
    97561 tgtacttctg ctgaatcgta cggcgtcttg atgtttcatc catcgctaga cgcatagaat
    97621 ctgttacttt gtctgcgtac atgattactt taccttcttc attccgagca gctcgtccaa
    97681 tcgtttggac taaggaacgt tcactacgta aaaagccttc tttatccgca tccaagatag
    97741 caacaaggga aacttcaggt acgtctaatc cttcacgaag caaattgatt ccaactaaca
    97801 catcaaattc acctaatcga agatctcgaa tgatttctgt tcgttccagc gttttgatat
    97861 cactatgcaa atatttgact ttgatcccta attctttgaa ataatctgtg aggtcttctg
    97921 ccattttttt cgtcaatgtc gttacaaaca cccgctggtc tttttcgact cgttcattta
    97981 tttcgcctac tagatcatcg atttgtccca tgattggacg aacttcgatc actgggtcaa
    98041 gcagccctgt cggacggatg atttgttgga taacggtatc tgtttgttca tgttcatatg
    98101 gtcctggcgt cgcagagaca taaatgatct gattgacatg tttttcaaac tcttctaacc
    98161 gaagtggccg attgtctaaa gcagatggca aacggaaacc gtaatctact agcatctgtt
    98221 ttctcgctcg gtcaccatta tacattcctc tgacttgagg catggttacg tgcgattcgt
    98281 ccgccacgat caaaaaatct tcaggaaaga aatccaatag cgtataaggc ggttcacctt
    98341 ctttacgtcc gtccatatgc cgagaataat tttcaattcc cgacgtgtag cccatttcta
    98401 acatcatttc aatatcgtaa ttggttcgct gttctaaacg ttgagcttcc agaagtttgt
    98461 tttcatttcg caagacagtt aaacgctgtt ctaattccgc tttgatattc gctacggcat
    98521 gctccatatg ttcatcattc gtcacaaagt gagtcgccgg gaagattgcc acgtgttccg
    98581 tttctcctaa aacttcacct gtcagtgcat tgacttctcg gatacgttcg acctcatctc
    98641 caaaaaactc cacacgtaag gcacgttcat ctcttgaagc cggaaaaatc tccacgacat
    98701 caccgcgcac tcgaaaacga ccgcgctgaa aatctatatc atttctttcg aattggatac
    98761 tgactaagtc acgaatcaac tgattgcgat ctagttctgc tccctgtcga atcgaaacta
    98821 cttgtttttg gtactcaaaa ggcgaaccta atccaaaaat acatgaaacg gatgcaatga
    98881 cgatcacatc atttctttct aataatgaac tagtagccga atgtcgtaat ttgtcgatct
    98941 catcatttac acttgaatct ttttcgatat acgtatcgct tgaaggcaca taagcttcag
    99001 gctgataata atcataataa gaaacaaaat attcaacagc attgttaggg aaaaattcct
    99061 tgaattcccc gtacagctga ccagccaatg ttttgttatg ggcaataatt agtgttggtt
    99121 tattcacttt ctcaataaga ttggaaatcg tatatgtttt ccctgttcca gtcgcaccta
    99181 ataagatttg tgcttttttc ccgccgacta cgccatccac taattggtta atcgcttcag
    99241 gctgatcacc agccggttgg tatttcgaga caagctcaaa gtgtcgggag gtatctctct
    99301 caatcattct tcgtcctcct tttcattcat tccttttcat tttacgacca ataaaaagaa
    99361 aaggaaagca aaggcattcc ttactaaaaa attataccac accgaacata ctttcgccac
    99421 ttttttatct tattatgcat gttacgtttt ataacataaa atgttagaaa ctcgcatttt
    99481 tttcattttt tcgagcttac agtcttgtct gattcagcac ttttcgccta taataataaa
    99541 atattggaaa tttaggaggt tgattatgaa aagaaagcat ttatttcact catttgtttt
    99601 cttaatgaca ggacttgtga cacttgcgtt tggacatgtt gcacaagctg acaacactac
    99661 aacggcagag tcaagcacaa gcgagacaat gcaaaaaata gagcccaaaa aagacgtata
    99721 cgtaattgcc agtgactctg cttttgctcc atttgaattt caaaacgcaa agggcgagta
    99781 cgaaggcatc gatgtaaaat tgatggaaaa catcgccaaa ctgcaaggat ttaacatccg
    99841 aatggactac cgtggtttct ctgctgcttt gcaagctttg gaatcaggac aagcagacgg
    99901 aatgattgcc ggaatgactg taacagatga acgaaagaaa agctatgatt tttctgattc
    99961 ttactttgat agcgggatcc aattagctgt caaaaaagga gatacttcga tcaagtctta
   100021 tgaagatttg aaaggaaaaa cagtcggagc aaaagttggt accgaaagtg ctgaattttt
   100081 aagcgaccat gaatctgaat atggctattc tatcaaatat tttgatgctg ctgatcaatt
   100141 gtacgacggg ttaaaagttg gcgcaatcga tgcaatgatg gatgattatc cggtaatcgg
   100201 ttatgcgatc aaacaaggac aagaattgga aacaccgatc aaaaaagaat ctggcggaaa
   100261 atatgcattt gccgttaaaa aaggacaaaa tcctgagctt cttaatatgt tcaatgaagg
   100321 attgaaagaa ttaaaaagaa caggcgaata cgataagatt attaaagaat atgtagcgga
   100381 tggttcagaa acttccagtg agtcttccac tgctgatgaa tcaacatttc ttggacttat
   100441 ccaaaacaac tatcaagcat tgttgaaagg actttggaga acaattctcc taactttgat
   100501 ctcctttgct ttggctatag taatcggtgt gatttttgga ttattcagtg tagcaccaat
   100561 acgcgctctt cgcacaattg ctagtattta tgtcgatatc atccgaggaa ttccaatgat
   100621 ggttcttgcg ttctttattt tctttgggtt gcctggtatc gttggattta cgattcctga
   100681 cttccttgct ggtgttatca ccttgactct taacgcaagt gcttatatcg cagagattgt
   100741 tcgaggcggg atcaacgccg ttcctgtcgg acaaatggaa gcttccagaa gtttaggtct
   100801 ttcttataat cggacaatgc aaaaaatcat tttgccgcaa gcgatacgca tcatgatccc
   100861 atcattcgtc aatcagttcg ttatctcatt aaaagacaca acgatcattt ccgcaatcgg
   100921 tgtagtcgaa ttgctgcaaa ctggtaagat cattgttgca cgtacaacac aaagtacgta
   100981 tgtttacttg atcattgcga tcatgtactt gattttgatc acagcattga caaaattagc
   101041 aaaagtttta gaaaagaagg tgaagtaaga tggcagaaaa aatcttagtt gagcatttag
   101101 tcaaaaaata cggcgataac acagtattaa acgacatcaa tgtgtccatc aacgaaggag
   101161 atgtcgtctg tatcattggc ccttcaggtt caggaaaaag tacatttttg cgttgcttga
   101221 atcgtttaga agaaccttct gacggtgaaa ttattattga cggcgcccac ttgatggata
   101281 aaagcacaga tatcaacttg gtgagacaac atatcggaat ggtattccag cactttaacc
   101341 tattccctca tctttctatt ttggaaaaca tcattttagc accgatggac ctgaagaaag
   101401 aatctagaga agaagcagag aaaaaagcaa tcaaattgct gcagacagtt ggtctagaag
   101461 ataaaaaaga tatgtatcct gaaatgcttt ccggtggaca aaagcagcgt gtggctattg
   101521 ctcgtgctct agcgatgaac cctgacatca tgctttttga tgagcctaca tctgcactcg
   101581 accccgaaat ggtcggtgat gtcttgaacg tcatgaaaaa gttagcagaa caaggtatga
   101641 cgatggttat cgttacgcac gaaatgggct tcgctaaaga agtggccaat cgtgtcatgt
   101701 tcattgacga cggaaacttt ttagaagacg gaacgcctca acaaatcttt gaaaatccgc
   101761 aaaatgaacg gacaaaagat ttcttgaata aagtattaaa tatttaataa aaaccaaaaa
   101821 tgcccggtcg cttcatgacg accgggcatt tttagtttct tgttttttca cttaactgta
   101881 ccagccgcat cgcttgttct cctgcacaaa caagcaatcg ctctacctgt tccatcgcat
   101941 aagaaagtac catcggttca ttgacgatat caagtacata gtcgatacca ccttcagaaa
   102001 ctatgatgat tatcttattc taaagctggc ttttttgttg gtacaaacat gacaactaca
   102061 aaaaa
//
LOCUS       DAYYCW010000002        73657 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000002 DAYYCW010000000
VERSION     DAYYCW010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 73657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.002"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(484..2376)
                     /locus_tag="ACHAYA_000096"
     CDS             complement(484..2376)
                     /locus_tag="ACHAYA_000096"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8603937.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2373..4100)
                     /locus_tag="ACHAYA_000097"
     CDS             complement(2373..4100)
                     /locus_tag="ACHAYA_000097"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8603938.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(4116..4766)
                     /locus_tag="ACHAYA_000098"
     CDS             complement(4116..4766)
                     /locus_tag="ACHAYA_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM8603939.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4970..5266
                     /locus_tag="ACHAYA_000099"
     CDS             4970..5266
                     /locus_tag="ACHAYA_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8603940.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5458..6078
                     /locus_tag="ACHAYA_000100"
     CDS             5458..6078
                     /locus_tag="ACHAYA_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8603941.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6170..7336)
                     /locus_tag="ACHAYA_000101"
     CDS             complement(6170..7336)
                     /locus_tag="ACHAYA_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8603942.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7348..7701)
                     /locus_tag="ACHAYA_000102"
     CDS             complement(7348..7701)
                     /locus_tag="ACHAYA_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8603943.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7829..8851
                     /locus_tag="ACHAYA_000103"
     CDS             7829..8851
                     /locus_tag="ACHAYA_000103"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM8603944.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8870..9031
                     /locus_tag="ACHAYA_000104"
     CDS             8870..9031
                     /locus_tag="ACHAYA_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603945.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9290..9724)
                     /locus_tag="ACHAYA_000105"
     CDS             complement(9290..9724)
                     /locus_tag="ACHAYA_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603946.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9834..11693)
                     /locus_tag="ACHAYA_000106"
     CDS             complement(9834..11693)
                     /locus_tag="ACHAYA_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8603947.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11694..11834
                     /locus_tag="ACHAYA_000107"
     CDS             11694..11834
                     /locus_tag="ACHAYA_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM8603948.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11811..12971)
                     /locus_tag="ACHAYA_000108"
     CDS             complement(11811..12971)
                     /locus_tag="ACHAYA_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8603949.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13278..14702)
                     /gene="araA"
                     /locus_tag="ACHAYA_000109"
     CDS             complement(13278..14702)
                     /gene="araA"
                     /locus_tag="ACHAYA_000109"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFM8603950.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14718..15416)
                     /locus_tag="ACHAYA_000110"
     CDS             complement(14718..15416)
                     /locus_tag="ACHAYA_000110"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8603951.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15373..17034)
                     /locus_tag="ACHAYA_000111"
     CDS             complement(15373..17034)
                     /locus_tag="ACHAYA_000111"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM8603952.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17252..18652
                     /locus_tag="ACHAYA_000112"
     CDS             17252..18652
                     /locus_tag="ACHAYA_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM8603953.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18904..19983)
                     /locus_tag="ACHAYA_000113"
     CDS             complement(18904..19983)
                     /locus_tag="ACHAYA_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8603954.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(20069..21028)
                     /locus_tag="ACHAYA_000114"
     CDS             complement(20069..21028)
                     /locus_tag="ACHAYA_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8603955.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(21040..22551)
                     /locus_tag="ACHAYA_000115"
     CDS             complement(21040..22551)
                     /locus_tag="ACHAYA_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM8603956.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22573..23136)
                     /locus_tag="ACHAYA_000116"
     CDS             complement(22573..23136)
                     /locus_tag="ACHAYA_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8603957.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(23142..24005)
                     /locus_tag="ACHAYA_000117"
     CDS             complement(23142..24005)
                     /locus_tag="ACHAYA_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8603958.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(24019..24915)
                     /locus_tag="ACHAYA_000118"
     CDS             complement(24019..24915)
                     /locus_tag="ACHAYA_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8603959.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(25090..26379)
                     /locus_tag="ACHAYA_000119"
     CDS             complement(25090..26379)
                     /locus_tag="ACHAYA_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8603960.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26351..27868)
                     /locus_tag="ACHAYA_000120"
     CDS             complement(26351..27868)
                     /locus_tag="ACHAYA_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM8603961.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            28066..28956
                     /locus_tag="ACHAYA_000121"
     CDS             28066..28956
                     /locus_tag="ACHAYA_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM8603962.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(29029..29853)
                     /gene="nadE"
                     /locus_tag="ACHAYA_000122"
     CDS             complement(29029..29853)
                     /gene="nadE"
                     /locus_tag="ACHAYA_000122"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM8603963.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29865..31337)
                     /locus_tag="ACHAYA_000123"
     CDS             complement(29865..31337)
                     /locus_tag="ACHAYA_000123"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM8603964.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31974..32336)
                     /locus_tag="ACHAYA_000124"
     CDS             complement(31974..32336)
                     /locus_tag="ACHAYA_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8603965.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32559..33311
                     /locus_tag="ACHAYA_000125"
     CDS             32559..33311
                     /locus_tag="ACHAYA_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8603966.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33451..34374)
                     /locus_tag="ACHAYA_000126"
     CDS             complement(33451..34374)
                     /locus_tag="ACHAYA_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8603967.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34389..34661)
                     /locus_tag="ACHAYA_000127"
     CDS             complement(34389..34661)
                     /locus_tag="ACHAYA_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603968.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(35005..36192)
                     /locus_tag="ACHAYA_000128"
     CDS             complement(35005..36192)
                     /locus_tag="ACHAYA_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM8603969.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(36605..38230)
                     /gene="groL"
                     /locus_tag="ACHAYA_000129"
     CDS             complement(36605..38230)
                     /gene="groL"
                     /locus_tag="ACHAYA_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM8603970.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(38281..38565)
                     /gene="groES"
                     /locus_tag="ACHAYA_000130"
     CDS             complement(38281..38565)
                     /gene="groES"
                     /locus_tag="ACHAYA_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM8603971.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            38790..39452
                     /locus_tag="ACHAYA_000131"
     CDS             38790..39452
                     /locus_tag="ACHAYA_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8603972.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(39528..40820)
                     /locus_tag="ACHAYA_000132"
     CDS             complement(39528..40820)
                     /locus_tag="ACHAYA_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8603973.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            40990..41619
                     /locus_tag="ACHAYA_000133"
     CDS             40990..41619
                     /locus_tag="ACHAYA_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8603974.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(41722..42531)
                     /locus_tag="ACHAYA_000134"
     CDS             complement(41722..42531)
                     /locus_tag="ACHAYA_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8603975.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(42586..43455)
                     /locus_tag="ACHAYA_000135"
     CDS             complement(42586..43455)
                     /locus_tag="ACHAYA_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM8603976.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(43456..44772)
                     /gene="yycH"
                     /locus_tag="ACHAYA_000136"
     CDS             complement(43456..44772)
                     /gene="yycH"
                     /locus_tag="ACHAYA_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM8603977.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(44769..46604)
                     /gene="walK"
                     /locus_tag="ACHAYA_000137"
     CDS             complement(44769..46604)
                     /gene="walK"
                     /locus_tag="ACHAYA_000137"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM8603978.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(46609..47313)
                     /gene="yycF"
                     /locus_tag="ACHAYA_000138"
     CDS             complement(46609..47313)
                     /gene="yycF"
                     /locus_tag="ACHAYA_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM8603979.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(47503..48363)
                     /locus_tag="ACHAYA_000139"
     CDS             complement(47503..48363)
                     /locus_tag="ACHAYA_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8603980.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(48350..48919)
                     /locus_tag="ACHAYA_000140"
     CDS             complement(48350..48919)
                     /locus_tag="ACHAYA_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM8603981.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            49300..49372
                     /locus_tag="ACHAYA_000141"
     tRNA            49300..49372
                     /locus_tag="ACHAYA_000141"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49333..49335,aa:Lys,seq:ctt)
     gene            complement(49654..50346)
                     /locus_tag="ACHAYA_000142"
     CDS             complement(49654..50346)
                     /locus_tag="ACHAYA_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8603982.1"
                     /translation="MNKKIVVSGLIIGGLVLAGIAGKNFADDILLWGGEDNIEQINHN
                     LESLDKALENKEQNISSLNSRLSSNNQELEQAKANVEEYKQKVLTLENDKNQLTIEKT
                     NLENQLSGKNAELQGKQNEINAKIDEINQKIAEINQLNTDWNNRLQTEQNKFNDAQNT
                     VNSLNQQINQLSNEKNTIKNQLQSTEQKLEKAQSENSNLNNYIEKLEKAKSDVEDTAN
                     RSQEIVTEHTGK"
     gene            50585..50731
                     /locus_tag="ACHAYA_000143"
                     /pseudo
     CDS             50585..50731
                     /locus_tag="ACHAYA_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(51056..51703)
                     /locus_tag="ACHAYA_000144"
     CDS             complement(51056..51703)
                     /locus_tag="ACHAYA_000144"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8603983.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            complement(51960..52979)
                     /locus_tag="ACHAYA_000145"
     CDS             complement(51960..52979)
                     /locus_tag="ACHAYA_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8603984.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            complement(52990..53187)
                     /locus_tag="ACHAYA_000146"
     CDS             complement(52990..53187)
                     /locus_tag="ACHAYA_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8603985.1"
                     /translation="MILPDKYYQVIKWTVLTVLPALSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            complement(53210..53503)
                     /locus_tag="ACHAYA_000147"
     CDS             complement(53210..53503)
                     /locus_tag="ACHAYA_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603986.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            complement(53540..53677)
                     /locus_tag="ACHAYA_000148"
     CDS             complement(53540..53677)
                     /locus_tag="ACHAYA_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8603987.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            complement(53679..54125)
                     /locus_tag="ACHAYA_000149"
     CDS             complement(53679..54125)
                     /locus_tag="ACHAYA_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603988.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(54122..54271)
                     /locus_tag="ACHAYA_000150"
     CDS             complement(54122..54271)
                     /locus_tag="ACHAYA_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603989.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPILNWKPKFNY
                     WEGNK"
     gene            complement(54288..56420)
                     /locus_tag="ACHAYA_000151"
     CDS             complement(54288..56420)
                     /locus_tag="ACHAYA_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM8603990.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKTNDRIDQTNQQIGDLGKLKKMYSNSIDFGDYD
                     YSGNPNLSAKLNASSFSLGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQY
                     TISCEIMLEDGFTGDPSGIRLQHAYLPGGVVVLQTETVPKNELNTWQTLIGTRTVKYT
                     SDVPNEWYPLFRDIRTLKPTGKVRLRKIKIEEGSTATPYQPNLLDDPYWLGKAPLGEN
                     IANKSVTFPIKSSAYSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRY
                     GNLEPVEGLVDTWGLTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGNTRTP
                     NISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEI
                     NSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSA
                     KLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQL
                     SIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            complement(56438..57262)
                     /locus_tag="ACHAYA_000152"
     CDS             complement(56438..57262)
                     /locus_tag="ACHAYA_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603991.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIGDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            complement(57266..58315)
                     /locus_tag="ACHAYA_000153"
     CDS             complement(57266..58315)
                     /locus_tag="ACHAYA_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603992.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGADEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            complement(58312..59184)
                     /locus_tag="ACHAYA_000154"
     CDS             complement(58312..59184)
                     /locus_tag="ACHAYA_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFM8603993.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSENVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            complement(59185..63030)
                     /locus_tag="ACHAYA_000155"
     CDS             complement(59185..63030)
                     /locus_tag="ACHAYA_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFM8603994.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIDKVGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NKVSDAIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGADKLEEWKVPQYAKGTPNGGHPGGPMMVNDGRGAE
                     AVITPNGQAFIPRGRNVVLNAPKGTHVLTAEETAYMTGNKAPRYRYAKGTGFFGNLWN
                     NVKGFAGDVGNKLKDVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFVATSGYRPGDPYS
                     HGKRNAIDIALPGVTGGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGIHN
                     DWRPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTAN
                     LNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFDP
                     LSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAKR
                     SRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKGN
                     SRTDRDLALDIQKILVRRM"
     gene            complement(63030..63221)
                     /locus_tag="ACHAYA_000156"
     CDS             complement(63030..63221)
                     /locus_tag="ACHAYA_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603995.1"
                     /translation="MDQIYKDLIESGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            complement(63245..63556)
                     /locus_tag="ACHAYA_000157"
     CDS             complement(63245..63556)
                     /locus_tag="ACHAYA_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603996.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     R"
     gene            complement(63683..64243)
                     /locus_tag="ACHAYA_000158"
     CDS             complement(63683..64243)
                     /locus_tag="ACHAYA_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8603997.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLAKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEEMTLQKFKDLVNNKAGE"
     gene            complement(64259..64630)
                     /locus_tag="ACHAYA_000159"
     CDS             complement(64259..64630)
                     /locus_tag="ACHAYA_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8603998.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EIFGNYEDFASNNPLTIQFNVQVDVWVSTLKEVDAFYFALDEIMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            complement(64627..65022)
                     /locus_tag="ACHAYA_000160"
     CDS             complement(64627..65022)
                     /locus_tag="ACHAYA_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8603999.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLQQNT
                     PYWDGTKSNGKRGSYMQEHAKDHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            complement(65019..65354)
                     /locus_tag="ACHAYA_000161"
     CDS             complement(65019..65354)
                     /locus_tag="ACHAYA_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8604000.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVRTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            complement(65358..65681)
                     /locus_tag="ACHAYA_000162"
     CDS             complement(65358..65681)
                     /locus_tag="ACHAYA_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8604001.1"
                     /translation="MILDPKMDLDEIKNALKIDTDDDDVEVRRAVQAAIAYIRGAIGN
                     DKPSFYKQENDTVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKARYL
                     LFVEE"
     gene            complement(65696..66892)
                     /locus_tag="ACHAYA_000163"
     CDS             complement(65696..66892)
                     /locus_tag="ACHAYA_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8604002.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRQFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     INPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSNWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIINVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTSIANE
                     VPVTNG"
     gene            complement(66889..67566)
                     /locus_tag="ACHAYA_000164"
     CDS             complement(66889..67566)
                     /locus_tag="ACHAYA_000164"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8604003.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGIDCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAETPQEETLLQKKLKA
                     LNGGKNE"
     gene            complement(67601..68821)
                     /locus_tag="ACHAYA_000165"
     CDS             complement(67601..68821)
                     /locus_tag="ACHAYA_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8604004.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDAEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALIKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGGNEGLKVKFASNVDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGSNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTNSSKSVAENG"
     gene            complement(68837..70189)
                     /locus_tag="ACHAYA_000166"
                     /pseudo
     CDS             complement(68837..70189)
                     /locus_tag="ACHAYA_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(70376..71137)
                     /locus_tag="ACHAYA_000167"
     CDS             complement(70376..71137)
                     /locus_tag="ACHAYA_000167"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8604005.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            71230..71388
                     /locus_tag="ACHAYA_000168"
     CDS             71230..71388
                     /locus_tag="ACHAYA_000168"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFM8604006.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            complement(71428..71868)
                     /locus_tag="ACHAYA_000169"
                     /pseudo
     CDS             complement(71428..71868)
                     /locus_tag="ACHAYA_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(71855..72376)
                     /locus_tag="ACHAYA_000170"
     CDS             complement(71855..72376)
                     /locus_tag="ACHAYA_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8604007.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            72479..72643
                     /locus_tag="ACHAYA_000171"
     CDS             72479..72643
                     /locus_tag="ACHAYA_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFM8604008.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            complement(72800..73117)
                     /locus_tag="ACHAYA_000172"
     CDS             complement(72800..73117)
                     /locus_tag="ACHAYA_000172"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8604009.1"
                     /translation="MKEARPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(73118..73639)
                     /locus_tag="ACHAYA_000173"
     CDS             complement(73118..73639)
                     /locus_tag="ACHAYA_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8604010.1"
                     /translation="MHIGDKIHKLRRLNNETMNDLVKQTGLSKSWVSDIESGKKQRAD
                     FNKICAIAKHYKVSLEYFQDDYLDSDEHEDIIPLTDETRVKSVKSLEQSLKYLNNKQE
                     PNLLEIKLKDTDSVPEVYYKGQKLEGLIDVSYHYHTTTDVVAGSTNDINMEYLGEVTK
                     YPDIKTIGHKRDV"
BASE COUNT        20764 a        15653 c        12584 g        24656 t
ORIGIN      
        1 tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttaagg aagaggttgt
       61 gaaaaagtcg cttagtttcg agtagtaaga aaaattttgt gaaaatggct ttctacattt
      121 ttgcataaat ttggcttaat accgagaaac tggcttttga acaccgttta ccaagaggtt
      181 gtgagcctgc cgtttaactc cgagtcacaa ggagcaattt caaaaaacag cttctcaggt
      241 ttgttgacta ttgtgacttg tgaccgagga gttggcagga gaacaccgtt taaggaagag
      301 gttgtaaaat cagcggtttg acctgagtat tagagataca aaacggaaaa atagcttctc
      361 atattttgcc gttttttgag ctaataccga aggacagctg attgaacacc gtttattcgg
      421 aaaaagggac tgtgacacga cttttgtcac agcccctaaa aaaaataata aatttgtact
      481 attctatgaa gcttcttctt cagaaaactg actgttataa agatctgcat agaatccgtc
      541 tttttccatc aatgtatcgt ggtttcctgt ttctacgatc gaaccatgat tcatcacaat
      601 aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca acaacgaaac ttgtccggtt
      661 ttccaatagg cggttcatcg ctttttgaat cagtatttct gttcgtgtat ctacactaga
      721 tgttgcttca tcaaggatca ggacatctgg atttgctaag aatgcccgcg caattgtgat
      781 tagctgacgc tgcccttgcg aaatattgct tgcatcctcg tttaatacag tgttatatcc
      841 ttctggcaat ttacgaacaa aatcgtctac gtgagcagct ttagcagcct caatgatttg
      901 ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca tagatacttc ctgtaaacag
      961 ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat tcatcacgtg aaagatcacg
     1021 ggtatctaca ccatcgtaac gaatactacc tccagaaaca tcgtagaacc gttcaagaag
     1081 gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca atagcgacca tctcaccagg
     1141 tttgacatcc aagttaaaat cagtcattag caagttatct ggtgtgtagc cgaattgaac
     1201 attttcaaaa cgaactttgt atggtgtatc tttttcttct ggaaggccag atttttcatt
     1261 ggacatctct tcttcatcca acacttcaaa tacccgttcg gctgaagcaa ctgttgattg
     1321 gatagtgttc atcaaacttg caagttgagt gattggttga gagaactgtg ttgtatattg
     1381 caagaacgct tgaacatccc ctaatgtcat gttcccattt gcaactttta ctccgcctag
     1441 tacagcgacg aacacatagc ctagattttt aacgaaattc atcaacggca tgatgattgc
     1501 cgaaataaat tgagctttcc aaccagcggc atacagattc tgattttctt tttcaaatac
     1561 ttcctggtct ttttcagtat gattgaatgt tttaacaatg gtatgaccac catatgtttc
     1621 ttctacttgg ttattcaata aacctaaact tttttgctga tctgcgaaaa acacttgtga
     1681 tttcggcgct acgatcatca cgatgatcaa gcttaaaggg atcatcaata acgcgactaa
     1741 cgtcaattgc cagctgattg aaagcatcat cacgatgacc ccaatcaatg tgactgcact
     1801 agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg atgttatcca tatcattgat
     1861 tgctcgagac atgatatccc catttgtatg tgtatcataa taggaaatcg gtacacgatt
     1921 cattttggct tccaaatcat gacgtaattc ataaactgtt cgttgggaaa cacgtgtcat
     1981 aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa tacatcacga ttactgtcgc
     2041 aatgatctgc gcgattttat caaagtctat cggaaatttt gttacttgtt ggccttgttg
     2101 catctgttga gcgccagcca ttacaccttt gaatatttct gtggttgctt tccccaagac
     2161 ctttggtgtt tggatttgga agatcacggc tgctattgct agtacaaata cagcgatgat
     2221 cgcaaccata cgtttagaca tatagccaaa caatcggcgt accgttcccc agaaattttt
     2281 aggcttttca ccttttacac cgagatttct gccaggtcca ccatgtcctg gaccgcctgc
     2341 tggtccatga gagggacgtg tgtttgtttt actcatgcta agtcctcctc tcttagttgt
     2401 gaatgaacga tttcttgata agtttcgttt gttgctaata gttcttcatg tgttcctttg
     2461 ccaaccagtt ttccttcatc tagcacaagg atcatatctg catccattac tgtacttact
     2521 cgttgagcaa ctaaaacggt gatactttct ttcatattcg ttttcaatgc ttggcgaaga
     2581 ttggcatccg ttttgaaatc caacgctgag aaggaatcat caaacacata gatatctgct
     2641 gttttgacta atgcccgtgc aattgccaga cgctgtcttt gtccaccaga gaagtttcct
     2701 cctccttgtt ccacatggct atctaagcca tcttccatct ctgaaacaaa atctttagct
     2761 tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg catccggctt accatactgc
     2821 atattgtctc ggatcgttcc agagaacaga actgcttttt gaggaacaaa gcccattaat
     2881 tctcgcaaat tatattgagt catgtcacta atgtccgttc catttatttg gatcgaacca
     2941 gattcgatgt catataatcg agggatcaaa ttgactaagg tcgttttacc tgaaccagtc
     3001 ccccctatga ttgcgacgat ctcgcccgac ttggcttgga aatcaatatc ttccaatgca
     3061 agtttttctg ctccatgata tcgataattg acatgattga aggccaatgt tgcgtttttt
     3121 ccagcaaaag aaactgtttt aggattttca gggtcatgga taccgatttt ttcatccaac
     3181 acttcattaa tacggtcagc tgaagcttgt gcacgaggca ccatgataaa aatagcggaa
     3241 agcatcataa agctgatcaa gatctgcatc gcataagtca taaatgcaat cagattaccg
     3301 acttccaaag tcatatctgc tatgtagtgt ccaccaaacc aagtgattgc tacgtttgta
     3361 ccgctcatga tcaatgtcat cattggcatc atcaatgcga cgatcgtatt aactttgatt
     3421 gccgtatttg tataatcttt attcgctaaa tcaaaacgat tttcttcaaa gcgtgtctta
     3481 tcgaatgctc gaatcactct gacccctgtc aatccttctc gaaataccag gtttaaacgg
     3541 tcagttttct tttgcatact tttgaataat ggaacagcaa agtacatgat tccgccgatc
     3601 aaaataatca ttactgggat gacaactaag aagatttttg tcaactgatg atctttttga
     3661 taagcaagta tgctcgcgcc aatcaacgtg attggtgcat tgatcatcat acgtaaaaac
     3721 atttgtgtaa ccatttggat ttgagtcaca tcattggtcg ttcttgtgat caaagaggcc
     3781 gttccatatt tatcaaacgc atcttttgtc agactctctg attttttata gacatccgta
     3841 cgcaattgct tacccagttt ctgtgattca cgagtagcaa aaaatgtatt tgcgatagca
     3901 gctaagatac tgatcaaaga aaagccgatc atcacaaaac ctgtatgcca aatataatcg
     3961 acatctcctt gcgctacccc tttatcaata atattggagg taagtgtcgg caaatataaa
     4021 tctgccagta cttggatcac catgaaaact gcagctgcaa ttgctgaagt caaggagatc
     4081 cgttttacga tttttagcat ctagttccct tctttctatt tatatactcc gtttttcagc
     4141 caagaaagtt tcaaattgaa ttcactgatt gcttgctcta ctgagtaatc ttcagatgtt
     4201 gaaaataagc gatacgaatg cgcaattgtt gtaaatacag tattcatcac taattggatc
     4261 agtaattcca attcatgatc attttttact ttcagcttcg tccgatcgat caattcaagc
     4321 aactcttttg catcttgttt gtgttcttta tgtggatcgt gaggcgtttt ttggcgactc
     4381 gtatgaggag cgacttttct tgaggcacgg taatccatgt tcataaacaa gttacgataa
     4441 aaagaagcat tctcgccttc caaaatctca taaatcattt tagaaaaata ggattcgaat
     4501 cctttgaata gatcaccatc ggattctttc attagttgta acatatcttt agagctgtca
     4561 cgtctcattg tctcaaagta ataaaaataa agatcctctt tatcttcaaa atactgatag
     4621 aaactgcctc tagggatatc cgctaatttg acgatattag caatcgaagc ttcttttaag
     4681 ggtactctag aaaactcgat tctcgcagcc tcgattagtc ttttttgttt ttctttaggc
     4741 aaatggaaaa atgtctgtgt cggcatatgg attctccttt ctttcaagat gacacattgt
     4801 catatgacgc tatgtcatta taacacctcc tacctgtttt gcaagacatt tgcatagttt
     4861 ttttcattat catccgatac attaaaaaag tgataagagg aatccttcca tttgaattaa
     4921 tgagagttgc ccattaaaat agccacatac taaacaaagg atgagataaa tggcccaatc
     4981 aattagtgtc caagacttca ataaattgcc tctcgatgct gacagcatcg ttttagatac
     5041 aagaaatacc ttagattaca caaaaaacca cttaagccac tcgctttcta tccccgctgc
     5101 tatcttgcca agacaattaa aaaatctaga tccaaagaaa ctttattata ttctaagcta
     5161 tacaggcagg cgatcagaag cattagccga tcaacttact gctaatggtt ttcgtgctgt
     5221 ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt gcttgaatat tgcttgttag
     5281 attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc ttttataccc tgtctttttt
     5341 tagttattta ttcttcaacc ttatcaatat gacttgatag ccttttcttt ttcgagtaaa
     5401 ctgattttgt tagtcatagt tataacaatc atagcaataa aaggagaagg taagcatatg
     5461 gcatctgttt tagtagtcaa aggtcatccc ctaactgcag aagaatcgcg cacagttaag
     5521 gcattgacgt cttttttaac aagttataaa gaaaatcatc cagatgatga agtgactgtt
     5581 ttggaacttt atcgcgatga tattccggaa atcgatgaag aattattatc aggttgggaa
     5641 gcattacgtg ctggtgcaga attcacttcc ttatcagaaa gtcaacaaca aaaaattgct
     5701 cgtttcaatg aattgactga tcagttcctt gctgctgata aaatcgttat cgcaaacgcc
     5761 ttatggaatc taaatatccc aacaaaatta aaagcttggt tcgataccgt caatgtagct
     5821 ggaaaaacgt tccgctatac agaaaacggg ccagaaggat tagtgacagg taaaaaagca
     5881 ctgcacatcc aatctaatgg cggtgtatac aacggacaag actttgcctc tcaatatgta
     5941 aaaggtattt taaactttgt aggcatcgat caagtagatc agctattcat cgaaggaatc
     6001 gattatgacc ctgaccgtgc agatgagttg atgcaaaatg cattagataa agctgctgct
     6061 ttaggtaaat cattttaaaa attgagataa atgcatacaa aagagacaga ggaaaaaatc
     6121 ttcaaagcag catttgtgcc ttggatattt tcccatctgt ctctttttgt tattctttct
     6181 gctttttcaa cggattttct tcataaagag aagagattgt tgtgtaccat tcaaaaacat
     6241 gagtaatcac tactttagct gctgcataaa caggaatccc caagatcaca ccgatgattc
     6301 cgaacaattt cccagaagtc agcagaacta ataaaatcgt tactggatgg attgctaact
     6361 gattccctag tactaatgga gagatcaagc gtccttctat tgtctgctct actgcaaaaa
     6421 cgatcagtac tttgatgatc atgaatggac ctgctacaat ggctaaaaag atagccggaa
     6481 tcatcgctaa aaatgatcct aagtaaggga tcaggtttaa aaaacccgca ataatcccca
     6541 acgtgacagc atactctaaa ccaatgaccg caaaaccaat cataaacatg attgccacag
     6601 caaaagccac agttagttgt cctcttatat aagaagaaac ttgactattc atttcactca
     6661 gcacattcaa cgtcggtttt tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta
     6721 attgtttacc gtctttcaat agataaaata agataaacgg catcgtcagc agagccacta
     6781 aaatagtcgc taccgcgccg acaaaacttc caagactctg aactgtactc ttagatatat
     6841 cttggatcat atcgcctgcc gaattcatga acttatcacc tgctgtttcc aactgttcgc
     6901 gaaattggtt gaacagcggg tcccgcaaaa tctcggttaa tttattatca atcgtatcta
     6961 catattgagg gaagttactg atgaagctca ctgtttgttc ctgaatctta gggatgatca
     7021 ctacgctccc ccatgcgatt agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa
     7081 gccttggtac ttttcgcttt tcaaagtaat caactaccgg attcatcaag taatacaaga
     7141 ttcctgctaa aatgataggc agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca
     7201 aatatgaaac ttttgtaaat aaaaagacga tcagcaatat taacaataca acaagtaagg
     7261 ctgtgactac ttgattattt aaaaaccagc gccaaaacca cgataccggt ctgttcttct
     7321 tattttttcc ttccataatt cctcctgtta gttataaaaa atttggcttc ccaaaccaat
     7381 agcaggcagt ctatgcggat gaagatagat tccatttgca atttgtcctt cttctggaca
     7441 agcatcaaaa atcaatgata cgtgagctac tgtctttaaa ttcccattgg ccatcggacc
     7501 gacataatcg atcgtatata cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga
     7561 aaaaggttcg tcttttgata cttcttgaat caccgaatat tttcgtaagg cagctgttgc
     7621 tgttttatcg aacaaaatca gcatgggttc ttccttgttt attgcttctg tcccaatttc
     7681 tgtaatcttt gcctgaatca ttgcactctc tcctttaatt gctttcaaaa acagtataac
     7741 acattctcat tctttagccg tgtggaataa ttatgtgata gaataaaaac gaaaactatt
     7801 tcattacaat taggaggaat cagctcatat gatcgaaaag ataattttag gaacctatac
     7861 gcgtcgcgaa agtaaaggaa tctatacgat cgaccttgat acagaaaaag aagaattagc
     7921 caacctttca ttagctgttg aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg
     7981 caatctttac acagttacta gtgtggatgg attaggtggt gccggtgcgt acgataagga
     8041 ttttcatttt ttgaatgcgg taacagagtc tggcgctcct ttgtgttatg tagcggttga
     8101 tgaagcacgt caattactat acggggcgaa ctatcataaa ggtgaagtaa atgtctacca
     8161 tatattagga gatggcgaac tgaaagcagc agattcaata ttccatcaag aagcgacagg
     8221 tcctcataaa aaccaagatc atgcgcatgt ccactatacg gatctgactc ctgaccaacg
     8281 attagtcgta tgtgatcttg gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg
     8341 caaattgaat cttgtaacta ccttcactgc agagccagga acaggccctc gccatcttgt
     8401 ctttcatcca aatggacaat tcgcttattt atttggtgaa ttagacagca cagtccgcgt
     8461 gctttcttat aataaagaaa caggtgcttt tgaagaatta cagaaaatat cgaccttgcc
     8521 agaagaattc gacggtgaaa atggaggggc agctatccgc atttcaaatg atggcaagtt
     8581 cctttatgct tctaaccgag gacataactc gattgctgta ttcgcagttt ttgaagatgg
     8641 ttcccacatt caaaacaagc aaatcatctc aacagaaggg gattttcccc gcgatttcgc
     8701 actagatcca agcaataatt ttgtcgtgtg tgccaatcag aacaccgata atctgacatt
     8761 atatcgccgt catcctgaaa caggattact ggaattactg aaaaaagatg tctatgtacc
     8821 agaatgtgtc tgtgtttatt ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca
     8881 agacgtatta gacagactac gggaagaact aaatattcct ttctttgatg ggaaaatcga
     8941 agataaagag tatagcgaag aggaatatca gaaactcaaa gccgatttac agaactactt
     9001 tgaacaatat gtacagaatg tagaaaacta atcagctttc catactatct gaaaagaaca
     9061 tcaggacaag acttttccgt ctatcctgat gttcttttct acaaataaaa aaccgccgag
     9121 atggttagac tcgacggaaa aggagtattt actcgatttt gagtaagtac aaggttgttg
     9181 ttggttatag gtatgtcatt atcataaagg attttgatca ctctatcata aaggtaatat
     9241 tacatttata ttagataact ctagttttta ttgttatcaa agatttcttt tattttttca
     9301 acaacaaatt agtcattttt tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt
     9361 ttgcttcaga tgtattgaat agatcttcat ctacccctga ttcaagatcg atactttgga
     9421 tcatttcttc acagcctgct acatatgaaa gataagcttt ttcaaaattt ttatgaatgc
     9481 ccattgcttt tgctggtgga cgcaaagtac tgactttcat aagcattgcc cgatattttt
     9541 ttgttccttc ttcaaataac aggatgatct cttcaagagt cgatttgtcc agttcacttg
     9601 tttttccttc atcaattgcg tttctgatca cttcatattt cggatgcatt ccttctccga
     9661 ttgcttctgt ttccttgacg atttgatcga tggactgcac ataatagcca atattcggta
     9721 acatgttcgt tttcctactt tctccattgt tgttattatt tattctactt gtttttatcc
     9781 tcaaatgaca agtacttgaa aaaatttaat ggtttctata cagctcgcca ttcttattcg
     9841 tgctcaaaga aaccatctgc taaactatat tgtttccctg cgacaatgtc atattggatc
     9901 aattgatatt cgtcgtaaat ctcttgttgc gagcgattga attgcgcttc tttataccac
     9961 tgtccgtttt gatagtacag ttctcgttca aacgccggaa gttcctgttc taatgataaa
    10021 agcagctgat aaaagccatt tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa
    10081 taaaacggac tggtgattgc atcgtgagtc gtttgctttt ctaattctcc tttactgtcg
    10141 aacataagaa attgtgtttc atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct
    10201 gaatagatcc ctggaagatg atccccccaa aaaacaacta aagtccttct agaaagattc
    10261 ttcaattctt ccgtgaactg cttcagtgcc gtgctgctat aggaaatatc ttgcagataa
    10321 ttctctaagt caagtgtccc acttccatct gaagtttttg caggatagtc tagattatca
    10381 tactttccat cgtagggcat atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt
    10441 ttttcttcct tgagaagtgt cattacttct ttgtaagctg actcatcaga aatatatgga
    10501 ttattctcta tcgtatccgt ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct
    10561 aatgtctgat aaacatcctc tcttttatac atcgaagtat tgtatggatg gatcgctgtc
    10621 gtctggtatg attgtgcgtt taacgctgaa acgatagaag gaagctgatc catttttggt
    10681 acaagcatcg tgtaaggggt ggtgagttgc gcattaaata acgccataga aaaaccagtc
    10741 aatgcttcaa attcgatatt tgctgttcca ccgccatagt tttgagaaag catattgcca
    10801 ctatatgttt gatcagctac ttcataataa tctgccaatg gctcaccaga tacttctacg
    10861 ccgtttaatc gactaggatc tgagaaactt tcactcatca cgaacacgat gttcggcgac
    10921 tcttcctcta cagttttatt tttttcatcg gctagcttct ggtatttttc agtgatttct
    10981 ttgatctttt ctttcgaata tccttcaggt tcatccattg gttctacctt cagattgtac
    11041 aaaaacccac cgataaaacc agtgttatag tagttcattt tctggctgta aggtatccat
    11101 agtgccgttt tattatatgc ttttcgcaac aaattatcgg gattgttgaa attactgata
    11161 taccctagca gtccaattgc cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt
    11221 ccttttttca ggcttcgaaa catgtaccag caaaacaatc ctatcacggt tcctgcggca
    11281 gctaagatca ctgtaaacag catagtgccg gtcatatctt tcagcaatcc gatttcagtg
    11341 atcatcttca aatcgtctgg ataaatcggt tcttgccgat aactcatttt catataattt
    11401 gcaaaaccta atacaccgat ggctacgaca tacgtaaggg aacctaaagg gacagatcct
    11461 gctagtgcaa tcagaaaaat aagaataatc agcaagacga gacaggctaa gaagaatttt
    11521 tccgtatgcc aagaaaaagc aaatttcaac gcaaggtcta cggatagatc attttgacac
    11581 cactgcaaaa ataaattact aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt
    11641 ccttttaaaa tctttttctg tttggtcgaa agtctgctta tatatctttt caaatgcttg
    11701 ctccttcatt tctatcaata aaagaatgtt gcttctctta ttttagttgt acgaaattcg
    11761 aaagtaaaga acggaaagaa gaattaatga aatccaaata ttctctttct ttatggcctt
    11821 tctggtttct ttaataagcg gacctttcgt actccagaaa ttttttctag ctgttgtttc
    11881 acttgattga gctgttcaaa atcagtactg tctatgtcaa tcaatatgta gacatgctca
    11941 ttcttatggt tacgcgatat atctgcgatc gctagctcgt tctcattcag cagagaaaat
    12001 atctctgcta atatattggt tttcttttga taaaatatag tgaatcgaaa cggcgcttgg
    12061 aaaaacattc gtgcggatgg ataattgacc gcttctctaa ccgtaccgaa caaaagataa
    12121 tcttttaagg cttcaactgc caaacgtccg ctgtctgcca aagcttcctg agtcgatccc
    12181 cctagatgag gcaacataag tatacggtca ttttctaaga actcttcttc tggaaaatca
    12241 gtcaagtatt tagccagatg ttgtttttcc aatgcttgca taacagcata tttatcaaca
    12301 atttcacttc ttcctacatt gataagtaca gcatcttttt tcattttttc tatattcgcc
    12361 tgatcgatca atccttttgt atcttctgtc aacggcagca tgactacgat gtaatccgag
    12421 gtagaaagaa ctgtttctaa atctgcttgc cgaacataat ccaactgggc atcgcggata
    12481 gaataaccca agacatccat ccctaagcta tagcaactta gcgctacttt tgtgcctatc
    12541 gctccaagac caagaagacc gatggttttg ccttgcaatt ctcgtcccac ataagcactt
    12601 cttttgtttt ctgcctgttc taaaatatta ggacctgtta gtgtttgtac catacgactt
    12661 gcttcgatta tcggacggga agataagagc aaacagcaca aaacaagctc ttttactgca
    12721 ttagcattta cacctggtgt attcatgacg atcgtcccgt tttcagaagc tttttcaacg
    12781 ttgatcgtgt tcaccccaac accagctctt gaaattgcta gcaaatctgg tgtaagccat
    12841 tcatctttca ctttcgtact acgaataact attccttcgg ctggcaaatc atctattatt
    12901 ttgaaatatt ccccttgtaa cttttctatc tcttgttctg gaatagcatc aatcaaatga
    12961 acgtgataca taaaaccccc tccttactaa caagtatctc gaaatttctg caattagaca
    13021 actgtttacc atttgacttc ctagaagcaa aaagagcagt tagagcagag taggctgagt
    13081 gataaaaata aggctatgac aagttactgc cataagcctt attttcgaga gcaaaatatt
    13141 gagggtgaga tatcttgata aatggtgttc cgttagctga cattgataat aagactaaat
    13201 ttcaaaaaag ttgaaaagca aatttcgtca atctcttctt atttcttaaa actaagtggc
    13261 tttttcacaa cctcttttta tctaataacc actgtttcca actcaactaa ttttgcccac
    13321 gtttcaattt gatctgttgt tagatttagc gagacaacgg tatgatgacc gccacctgcc
    13381 tgaatccatg aatggacacc ttcatggaaa ttcggtttag ttttccataa cacacgtgca
    13441 acaggtaaat taggtgcttc agtcgttggt tcaaaggctt ctacttcatt gatcagcagt
    13501 ttataatgcg taccgaaatc tgccatcgat acgactaccc cttctcctgc tttcccatca
    13561 aatacaagac gcgccggatc ttctctatct cccattgata aaggagaaac aacgattctt
    13621 ggtttcgtcg cagcaagcgt cggatctact tccatcatat gggattccaa gattgcttct
    13681 tggccactag ctagctcgta agtataatct tccatgaatc ctgtatcttt ccctcgagcc
    13741 atgattttca gcaaccgatc aatcgctgca gttttccagt cgccttcccc agcaaaaccg
    13801 tatccttctg ccatcaaacg ttgtacagct agtccaggta gttgtttcat tccatacagg
    13861 tcttcaaaat tagtcgtaaa agcgttatag cctccttctt ctaagaaacg gcgaataccg
    13921 atttcctgct gagcttgtac ttttacatgt tcctcccatg tcttttcttc ataatcacca
    13981 tagtcgaagg tatacaattc cttgtactct tcaaataatt gatcgacttc ttcgtctgat
    14041 acgcgatcga ttacttgtac aagatctccg ataccaaaat aatcaacagt ccaaccaaat
    14101 tgaatctgtg cttccacttt gtctccttct gtgacaccca cgtttctcat gttgtcacca
    14161 aaacgagcca cttttattcc aaaactttca ttataggcta ctgcaacatc catccaatca
    14221 gcaatctctt tttggatctc aggtcgtttc cagtagccaa cgacgatttt attttgttta
    14281 tttaatcgag cattgatgaa cccatattcg cggtcaccat gagcggattg atttaaattc
    14341 atgaaatcca tatcgatcgt tttccaagga atgctttcgt tatattgcgt agctaaatga
    14401 agcaatggtt tttgtagaag ctttgtcccg cggatccaca tttttgctgg tgagaaagta
    14461 tgcatccaag tgatcacacc tgctacttct tctcgataat tgacctcttt catgattttt
    14521 gtgatcgtat cagcagtaac tgctaattct tgaaggcgga ttggataagg caattggcca
    14581 ttttcattca gttcatcgac catttcttgt gcatgtttct ttacttctcg cagtgcttct
    14641 tccccatata aatgctgtga tcctacaaca aaccaaaatt ctttttctcc aatattcaac
    14701 ataattagcc actcctttta tttgttttgt ccgtaatacg cattttttcc gtgttttcgt
    14761 aaataatgtt tatctaaaat ccgttgaggc agctcttctg aaaatgaatt gagctgtctg
    14821 gtaaataaat tcatcttgca tacttcatct agtacgactg cgttcattac cgcactttgc
    14881 gcatctttcc cccaagtaaa tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag
    14941 ggatcaattt tgcgttcttt gaatgtttct atgataactt ttcctgtttc atattcgtac
    15001 ccgctgtcga tttcctgttg tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta
    15061 tctgcatgcg tcgtccccat cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc
    15121 catgtcgagt gagtatggac gattcctccg atttgaggaa atgaacgata taaaacagca
    15181 tgagtaggcg tatccgaaga gggattgagt ttaccttcaa tgatatgatt gtccaagtcc
    15241 acaacgacca tgtcgtctgc tgtcatctct tcataactga ctccgctagg tttgataacg
    15301 aaatatcgtg tttcaggatc aaaagcgctc acatttcccc atgtatattt gacgagtccg
    15361 tgtttaggca agtcaagatt tgcttggtat acttcttctt tcagttgttc cagcattttc
    15421 tatcactcct ttttcttgca attgatgaat cgctgttttt tcaatgtcga tcccctcacg
    15481 gtaacgtctc ataaatatct gatagcctgc tatttcttct gctgaaggtg tgatggtgat
    15541 tccttggtct tgtccaaaca cgtcttctgc caagaagttt tctaaagact tcccatgtcg
    15601 atccatcata taggctgcta ataacgcgat tccccacgct ccaccttctc cggctgtgtc
    15661 catcactgta actggtgcct ccatagcaga tgctagaatc cgctgtccaa cttctggtgt
    15721 tttaaaaatc cccccatgcc ccactaaacg atcaatttgg acattctcag atttcaatat
    15781 atccattccg attctcattg caccaaaagc actggctaaa tggatccgca tgaaattcgc
    15841 taaatcaaaa cggctacctg gtgtacgtac aaataatggc cttccttcat ttactcctgt
    15901 aatgttttca ccggaaaaat aaccataaga aagtaatccc ccgcaatccg tatcgccttc
    15961 tagcgctttg ttgaataata ccgtgaataa tttatctctg cttatctcca tacccaaaga
    16021 attggtaaat tcttcaaaaa tactgaccca agcattgata tccgaagagc agttattcgt
    16081 atgaaccatg cctactagtt ctcctgaagg tgtagtgacc atgtcgattt ccggatggac
    16141 attttctagt tctttttcta atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt
    16201 ccctgtccct ttggctacac tgttcgtagc gaccataccg gttcctgcat ccccttctgg
    16261 cggacaaacc gggattcctg gtctcaaatt tcccgtaggg tcgatcagat aagcccctga
    16321 ttcgcttaac ttgccagctt cttctcctgc tactagaact ttaggcaaaa tgtgatccaa
    16381 gtcaatcgct acgtgcgctt tttcgattaa ctgaccaaaa atatttttca tttcttgatg
    16441 gtaggttttc gttgaaggat cgataggaaa catgcctgat gcatctccta cgcctagtac
    16501 tttctcacca gtaagctgcc aatggacata acctgcaagc gttgtcaaaa aattaatttc
    16561 tttcacatgt tcttctttat tcaaaataga ttgatacaaa tgagcgatac tccatctctg
    16621 cggaatattg tattgaaatt tttccgttaa tattcgttct gcttcttcag tgattgtatt
    16681 tcgccaagta cgaaatggta ctagtaactg ttccttgcta tcaaatgcta gataaccgtg
    16741 catcattgca ctgaacccaa tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa
    16801 tacttctgct gccatttttc ggtaactgac gcgcagtcct ttccaaatat cttctaatga
    16861 ataggtccag ataccatttt ctagtttgtt ttcccactca taactaccag cggcaatagg
    16921 agtaaaagat gaatcaatca atacagcttt gatcctagtt gatccaaact caatccctaa
    16981 agatgtacga ccatctatga catctaacat actttgcgca tttacttcct tcataatgat
    17041 ttaactccct ccttctcgtc aaacctgatc aaaccgtctt tttagtagcg cttacaatga
    17101 ttattgtata cttttgtacg tacatgtcaa gcagttattt acaagaatac aataaaaaac
    17161 acttgttttg tacgtacata agttataaaa tgaaattgct tacaaaatct gatcaaccgc
    17221 cttctcgaaa ttctagaagg agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc
    17281 gtttatttat ttttttggcg catttggcgg cattctattt ggatatgata ttggtgttat
    17341 gaccggcgcc ttaccttttt tacagcatga ttggggtttg gcaggtaaag cttctttgat
    17401 aggatggatc acgtcttcgg tcatgttagg tgcgatcctt ggcggctctt tatctggtct
    17461 tctttctgat aagcttggac gacgaaaaat gattcttctt tctgcactta tttttatggc
    17521 tggttctgtc ctttctgcat ctgcccctca caatggttct tattttctga ttgcagcaag
    17581 gattcttcta ggtttagctg ttggcgctgc atctgcccta gttcctgctt atatgtctga
    17641 aatggctcct gctagactaa ggggaagatt gtcagggatc aatcaagtga tgattgcttc
    17701 tggcatgctc ctttcctatg ttgcagatta tttgctaaaa gggttgcctg aaacgatggc
    17761 ttggcgcgtg atgttagggc tcgctgctgt ccccgcgctg attttatttt tcggtgtttt
    17821 agctctacca gagtcaccgc gttttcttat gcaatctggc cgcttggaag aggcaaaaag
    17881 agtattgaac tatattcgga caccaaaaga agctgaacag gaattcgaac aaatccagct
    17941 gaatgtcaaa caagaaaaga caaccggaac ttcttggcat actttgtttt tagaaaaata
    18001 tcgttcctta gtcattgccg gtattggtgt agcagtattc cagcaatttc aaggagctaa
    18061 tgctattttt tactatattc cgctgatcgt agaaaaagcg actgggcatg cagcgagcga
    18121 tgcattgatg tggccgatca tccaaggaat cattttagtt gctggttctt tgctcttttt
    18181 ggttattgca gacaagttca atcggcgaac tttattgaaa ataggcggta gcgtgatggg
    18241 actttctttc atcctaccag ctgttttagg aactgtactg gatgctcata caaattcgtt
    18301 gttgattctc ttgtttttat gtatttatgt cgcattttat tcttgcacat gggctccact
    18361 gacatgggta atcgtaggcg aaatattccc actagctgtc cgcgggcgag catctggcct
    18421 agcctcttct tttaattgga ttggttcttt cctagttggc ttgctttttc ctgtgatgac
    18481 tgcttctatg tctcaagaaa tcgttttcgg catttttggg atcatctgct ttttaggggt
    18541 tttatttatt caagagatcg taccagaaac tcgtgggaaa tctttagagg aaatcgaaca
    18601 aagtgcatct aaaaaaactt atcctaaacg tatttctatc catcattctt aaagaggttg
    18661 tgacagaagt ggtcagcttc aagaaataag aaggaaccgc ttagtttcca gtcacaagga
    18721 acaatttcaa aaaacagctt ctcatgtttg atgaatattg tgacttgtga ccagttggta
    18781 ggagaacacc gtttagtgga gaggtggtga aagaaacgtt cagccttgag cgatgacaaa
    18841 agctctcaca aacattgcga cgagtaaccg taggagcaat aagaggcggt aaaaacccgt
    18901 ctcttatttt tcgttttctt tcactgtttc ccgttcaact agtacaggtt catagagaat
    18961 atttggctgt gcctgtcctt tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc
    19021 caacttttct tttggatgcg tcaatgttgt caatttttta ttccctaaat ggcttaaaga
    19081 agaatcgtct gttcctacga tcgataggtc atctggaata tgatagccat tttctgtcaa
    19141 cttatctagc attaaactag ctatttgatc gttatagcaa atgatgccat ctaaagcttg
    19201 ttcttctttc aaccgttgta ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa
    19261 agtaatgata tcttccgtca aaaaacgcac attatactgt tcacacgcgc tgataaaacc
    19321 tttcatcctg tattttcctt gaagatcgtc gatttttgtg atgaacaaca gacgctgatg
    19381 tccttttttt atcaaatatt ctgctgccat aaaaccgact ttcacatcat ctaaacaaat
    19441 ataagaggca tttaactctt cataagcagc attgatcatc accatcggaa tgccttgttc
    19501 tctcaaagca acataaagtg ctaagtttgg gttatactga ttacttttcg taggctcaac
    19561 gatcaaacca tctaccccat aagcaatcat tttcttcaaa cactctttct ccaattcgtg
    19621 atcgttattc gtactggcta aaagcaatga atacccagat tctcgtaatt cattttcgat
    19681 tcctcggata atcgacggaa aaatataatc agagagatat gtggtgatga caccgatcgt
    19741 ttttgttctg ttcaatggtg ttttttcttt cttatagctg tcatctacat atgtgcccga
    19801 ccctttttct ttacgcagat agccctcatt taccaacaca gcgattgctt gccgaatcgt
    19861 atgtctgctg acgtcatata tttcttgcag cttgaattct gagggaatgg ttgtatttac
    19921 ttcgtattcc tcacgaagga tcttttgttt tatctcttct gcaatatctt gatattttgc
    19981 catttgcctt gccccttttt atttgtacgt acatatatca taacgagtct gcaaggttta
    20041 ttcaagcaaa aatcaaggtt tcttgtgctc ataaggcata cggaacaggt tcaccaaatt
    20101 ctggaaatcc ttgctcattc cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt
    20161 ctaacggatc ccctttttgg ttcttctctg gtcgagcatg atagatcaaa atatcaaagc
    20221 acccgtcttc tgaaaccgta aacgagttgt gaccaggacc aaattgctta ttttttttag
    20281 aagaaacgaa tactggctcc gataatttat gccatgattc ttttaataaa agattcttgt
    20341 gctcatcagc ccacagcagg cccatggcat agttttcatc tgttgcacta ccagaatacg
    20401 taatgaatat tttcccattg cgagtaagga ctgctggacc ttcatttact gaaaaacccc
    20461 gtttttccca ttcatactca ggtatagaaa ggaggatctg cttccccttt aacgtccaag
    20521 gattatccat ttcggataga taaagattag aattcccagt aatttcagga tcttgttggg
    20581 cccacacata atatagcttt ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt
    20641 cccttttcgt ttttatttgt cccttttcta tccaatctgt attcattgga tctatatgct
    20701 gattttcaag tacgaacatg cggtgatgat gagattcatc acgtatagtt ttatgattgc
    20761 ttgcagcaaa gtaaatatac cattttccat tgatataatg gacttcaggt gcccagatca
    20821 attcactcat cggtccttct tcatgagcct gccatacaat caggcttgca gcatgttcta
    20881 attcattcag tgactttgcc cttcttaatt caatcacttg gtagcctgga accgatcctg
    20941 taaaataata ataaccatct gtatgtttat atacccaagg atctgcccgt tgccgaacga
    21001 taggattatt ataaagtgtc ttcatcataa caaatttcct cacattttct ttattctaat
    21061 tacattccaa gagtattttt ctatccctac atttaattcg tcatgttttt ctattgaaaa
    21121 atagtttatt tcaggtttta ttttttctgg ttcttcacgt gtatttttgt ccaacagatc
    21181 gctactggtt aatgtgtagt gctctccttg atctagcaaa ttgaaatcat gttcaatcgt
    21241 tatcttttgt tgttcatttg tgcgattgat caagaaaata gtcagctgat tgccgttatc
    21301 tacgatcaca ctgtccacat aaggaacatt ttttatcttt tcacaatcat aaacagggca
    21361 aatgatttgt tgtttcaata cttgaccttt agcatatttc gataagtgtt gcatcacata
    21421 ataagttgga ttcctccatg cttgtccttt agcatctgtc agtattaaag gtattgtgtt
    21481 tactaacaat gattgacaac taatctttac gcgatctgcg tgtcgtaaaa tagccagccc
    21541 taatgaacca aataataatg tatcttccat agtaaagtaa caccaatcga tgggactacc
    21601 gacttcaaaa tccctatgtc ttttttccgg ttgggcatgt acattccatt catcaaacgc
    21661 tagatacatt gttttctcac ttctcaaata tccttttaca taatcacaag tagcaacgat
    21721 ttcttcaatt tgacgatcga atcgataagc ttttgcaagg tatgtcgctg tatcatcacg
    21781 ctcattttta ggaggtttag tcaagtcttc ttcatcatac ttatcaatat agttatgcaa
    21841 agctaaataa tctacattct cataagcttc ttctaatgcg atacgatccc attcagggta
    21901 cgatggtaaa cgtggcgtag agcttccaac taagattgtt tcaattgaat catccactaa
    21961 cttcatgact tttgaggctt catttgctaa cctaccatat tcataagcac tcttagcccc
    22021 cacttgccat ggaccgtcta gctcattccc taaacaccat aatttaacgc caaaaggctt
    22081 tttttgccca ttttttattc ttaaatcact ccagaaggtt ccttttggaa aattacaata
    22141 ttctactaaa ttttttgctt catcaattcc ttttgttcct aaattcaccg tgaatatcgg
    22201 tttcgcttgt acatgactga tccatttaaa aaattcgtgg agtccaaaag aatttggttc
    22261 tatacctcgc catgctaaat caattttttt tggtcgttgt tctttcctac ctacagtatc
    22321 cacccaatca taaccagatg taaagtttcc tccaggatag cgaatgacac ctaattctaa
    22381 ttcctttaca taagagagga catcttgtct aaacccgtca ccatcagctg tttcatggcc
    22441 cggttcataa atccctgtat agacgacact tcccatatgc tcaacaaatg agccaaacag
    22501 ccgagaactg atctctccta ccgtaaaatc ctctctgatt tttactttca cttcttcgtc
    22561 caacagcttt ccctaccttt catttcctat agtcaagata atagatacaa gtttccatac
    22621 gccaccagca aagataaaaa taaaaggagg gaaaagaaat atgagcagta caaatcctaa
    22681 taacaatacg caacaaagaa tatttctcaa aaaatcaaaa aatactttcc atgcactttg
    22741 ttcaaaaatt tgtaaaaatg tgatatcttt gttttcagaa atcacatata tccaggataa
    22801 aagaaacatg gaataaataa gtaaaactac atatccacta attgaaagta cgattgtctg
    22861 ttcttctaat aattgattaa caagaaaata aataatgatc gttaacagac taaggaacaa
    22921 gttcttacca aaatgttgct ttattccttg aaagaaaaaa taaatgatat ttcctgatcc
    22981 ctcacctttc cattgcgcca ttacataaaa aacagcacat gtagcaggca cgatagtaat
    23041 aacaggcaaa cacacgatta cccagagaat acctaataag atgtaatcag aaaccacaat
    23101 caactgtcca taaagtttag tctcaactat tttcatcttc ctcactcttt cattccagac
    23161 atagctgttc cttgaacaaa atatttctgt gcaaaaatat aaaccactag taatggcagc
    23221 attgaaataa ccgttccagc cattacaggt ccataaaaag aaatgttctg ccctgtaaat
    23281 aaagctaacc ctgctggtaa agttctcatt tttgaactac ttgtcaatat caatggataa
    23341 agcaagtcat tccaactatt catcagcgtg aaaatcccta atgataaaag agtaggtttg
    23401 ctcaatggca acatgatttt caaaaaaata ttaaactcac ttaatccatc aatgcgtgct
    23461 gcctcttcca aagaatctgg taaagtaata aaaaaggaac gcatcatgaa gataccaaat
    23521 gcagttgtca tacgaggaat gatcaaacct gcataagtat caagcatgcc aagcaaattg
    23581 acttcaataa atagaggaat catgaagact tggaatggga tcatcattgt cagcaaaacg
    23641 aaatagaaca aaactgattt tcctttgaaa cgcatacgag caaatgcata ccccgcaaga
    23701 gaatcaaaga atacagaagt gatcactgtc cctccagcaa aaatcactgt atttttgatg
    23761 taatctaata aagggatcgt tgtccaaacc ttaatatagt tatctagggt atagctatct
    23821 gcaaatattc taggcggaaa tgagataatg tctttttcgt gtttaaaaga agaaataatc
    23881 agccacaaaa aaggaaacgc aataaccaac aatataacta gcatactaat cgttcgaatg
    23941 atttttttac cactaactgc ttttcgacta ttcacagtaa ctgtaatcga gctttctggt
    24001 gccatacagg tttccctcct atttcatttg tttttctttc cgtgacatgt acacattagt
    24061 ggagatagct atagttgcaa caatgataaa caaaaccgaa gacagtgctg atgcatagcc
    24121 caattcataa ggtgctgtaa atcctcgact gtaaatatat tgaacagctg tttctgtctt
    24181 aaattgtggg ccaccagcgg ttgcaacata tacttggtca aacacttgca tcgaaccaat
    24241 cagattcgtt aaaatacaaa aacctaagga gggaacgatc tgaggtagcg taatgttaaa
    24301 aaactgctgt cgatttgttg caccatccat ttgagcagat tcatacaaag agggagagat
    24361 tccctgaata gcagtcaaga gaatcgtcat agtaacacca aaattcttcc aaacagtcat
    24421 gatggctacc gtcggcatag caagggttgg atcgcgaaaa aattgaggga tcggcaagcc
    24481 gacttgtgaa aacaagtaag gaatcgcccc tacattcgga tcaagcaaca tagaaaaaat
    24541 aatcgcaatt gacgtcaatg aacaaataac tggaatgtaa aatgtcgaac gacaaaaacg
    24601 attgaatctg gtatttttag ataatgctac tgctgctaat aaaccaacga taatttgtgt
    24661 tggcgtttcg agtacggtga aatataaaga atgaatcaac gaattaactg ctcgttcatc
    24721 actaaatata cgtgtgaaat tttcaaaact tacaaaattt cttgatgtaa agaaaatatt
    24781 caaatctgta aaactaatag caaatgttcc taaaagcggt atgacagtaa ataataataa
    24841 aactaaaaaa gatggagtta aaaacaaata cgcagcacga tcaggacgat tccaatattt
    24901 ggtgaatccg ttcatatagt ccacctcatc tttcattttg ataaaatata caatcttggt
    24961 ttaaacgaat ctgaataaga tatccgaaca atcgctgtga tttttgcaat aacacctatc
    25021 tgcaaatact aggtcgatca ttcagatatc tgtatcttgc caacagtcta tcctgtataa
    25081 agttctgtac tatttttgca gtaattgatt gatggcatca tctgcatcgt tcatgagtgt
    25141 ctcaggatct tctcctgcta ataatttttc aatcagtgga ttgatgatat cgttattgat
    25201 cgcagagatt tgggtaaaac ctggtaaata tggctctgca tagtccattt gtttcgataa
    25261 ttcagataca ataggatcat tttttacttc ttcatctttt gctactgaat ccaagtaggc
    25321 aggaaaacca ttttcgatac tccatttttt accaatttca gttgtattcc aatatttcaa
    25381 aaactcatag acagcttctt ttttattatt atctgtactt gccggaatgg catatcctac
    25441 gccatctaaa atagctgttt ttttgtctga atcactccct tgtggtaagg tagtcacatt
    25501 ataatcaatc tcgtttgctt tcaatccgtt atttaaccat ggcccattga tttcaatggc
    25561 tagttgacca gcattcatta aattgtccat ttcggcgcca gaaatcgatt ctggaccagt
    25621 cttgtctaca tggatcattt tttggatcgc ttttaacgtt ttaaggttct caggagacgc
    25681 aaattcagac ttagtataat catcgttcac taatttaccg ccatttgcta acatccagtt
    25741 atataacggt gcagttccat ccttattgaa gacaaaacct gatacatttt tactactatc
    25801 agttaatttt ggtgccattt ctgtcaattc ttcccaagta gtaggtggtt gttcagtatc
    25861 taatccagct tgtttaaaca aatcattatt ccaatacata tacatgcctt gtacctgcat
    25921 ggggacaaaa tactgtttac cttctacttc acctaattca atagccgttt ctttaaaatc
    25981 agatttatct actccatcat attcccagaa atcgtctaat ggttgtaccg ctccgttttc
    26041 tacatactgt gcaaaatctg gataatttaa tgcaataaag tcaggtgctt ttttagaaga
    26101 aatcgctgta ggaagttttt cattcaaatt ggcccaagtc atgatatcca tttcaatttt
    26161 gatgttttta tcatttgttt tattaaattc attaacgatt tcttgcaata tttcaccatc
    26221 agatgcagta aacccattcc aaaaagtcaa agtcacttct ttatcagaat cagattctgc
    26281 tgaacttccg ccaccgcaag ctcctaaaat acctgttgca gctaccaaca aagagccaat
    26341 taaccatttt ttacctttca ttttttgttc ctccttcatc tgataaacga catctgacca
    26401 cattccaaga tagcggtttc aataaacaat tgattttccc atcttcagct tttacatttg
    26461 ttgaatgact tggttttact tgttcacttt taggaagatt agttgctttt gtatcaaatc
    26521 cactcatttc agaaaaatca atcactccct ctaatgataa ttcagacaag tgaacagtta
    26581 gattcatttc ttcttctcca cggttaactg caaatatgac aagttctttg tcatttatta
    26641 cggctatact ttctaaatac ggcacttctt caaaatcttc cgtcgcatag tttgctgaac
    26701 gaatttgtgg gcttaacacc tctccttgtc catacacagc agcttgcata aatggataat
    26761 agatcgtctg tctccaagct tctccgtttg tttctgtcct gattggtgca ataacattta
    26821 ccaattgagc aagacaagca atctttactc tatctgcatg tttcaataaa gtgattaata
    26881 gacagcctaa tagtaatgca tcttcaaaat tatagttatc ttctagtaaa ggaggagcaa
    26941 tctgccaagg cgctgcttgc tcatcttgtt ccgctgaatg ataccaaata ttccattcat
    27001 caaaggacag gttgatctgc ttcttactat gtttcttcgc cttgactgta tcgcacatcg
    27061 ctgtcacttc agaaatgaac cgatccatgt ctaaggaacg tgcaagataa tgagaaagat
    27121 catttttttg gttgccataa tattgatgca gagacaaata atctatttga tcgtacgctt
    27181 gatcaagaac tgttaactcc caatcaccaa atgtaggcat ttgccgattc gaacttccgc
    27241 ataaaaccac ctcaattgaa tcatctacga gtttcatcgc cttagccgtt tcctctgcta
    27301 accgtccata ttcttccgct gttttatgtc caatctgcca aggaccgtcc atttcattcc
    27361 ctaaacacca tgtcttaatt ccaaaagggc tctcttgacc attcgaacgc cttaaatcac
    27421 tccaataagt tcctttagga aaattacaat attcgactaa attacgagct gcatctactc
    27481 cacgagtacc cagattgaca gccatattga tttcggcccc cacatgctga caccagttat
    27541 aaaattcatg aatcccaact tcattcgttt caattgtccg ccaagctaaa tccaaccttc
    27601 taggtctatt tgctatgggc ccaatactgt cttcccaacg aaatcctgac acaaaatttc
    27661 caccaggata tctaatcatg ggaatgttca attcttttac caagtgaatg acgtcttggc
    27721 gaaatccact tttattagct gatggatgat ctggctgata aatcccttca tatactgctc
    27781 gtcccatatg ttcaataaat gaactgtaca accgcggatc aactttgcct acacgcttga
    27841 cactactaat caacattgtg gcttccataa ctgacctcct ttaatttata catgaataat
    27901 acacctcgtt gaaagcgttg tcaataatat aaatgttaaa cttacttatt ttttatcgat
    27961 tttaattaac caaaatatta ataatgatta aaacaaataa tttaatataa cttttattaa
    28021 caaatatgtt aatatataag aaaaaccttt taagaggtga tatatatgca actacaaata
    28081 aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat caagatcatc
    28141 cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact tggagtgagc
    28201 agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat caaaacagaa
    28261 aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat cagttttcca
    28321 gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg tcactataca
    28381 aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat cgggaaagtg
    28441 gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct ctggtttacc
    28501 agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac attggagatg
    28561 ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa ttggccttca
    28621 gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc tggagatttt
    28681 gctgatatcc gcggtaaata tacgcctgat tggtatccag ataatcttaa tcaatacggc
    28741 ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga acccttgtca
    28801 aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg aatcgaagtg
    28861 aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt cggtaattac
    28921 gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct attttataaa
    28981 acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt atttccagaa
    29041 atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc accatgcttc
    29101 gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat caatttcatt
    29161 atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt caagatctgc
    29221 agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca attgtttccc
    29281 ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac caaatttcgt
    29341 gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc ctgctctttc
    29401 tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc ctttgttaaa
    29461 atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa cagctggttt
    29521 gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt ttgcatcttc
    29581 ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt tttcatcttt
    29641 tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta aagtagaatc
    29701 ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt tcaaatatgc
    29761 tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg gtttgacacc
    29821 taattcattg ataatttctg tctgcaggct catttattct cctcctatgc ttctgtatga
    29881 agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt ccagcattca
    29941 gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc ttcccatagt
    30001 gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa ttcgtaaact
    30061 ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc tcgaacagtt
    30121 ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc tcgagggtct
    30181 tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg agtgatgcgc
    30241 cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa cttgatcgta
    30301 tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc accaagggct
    30361 ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat tttagctttt
    30421 tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata gatttttgct
    30481 tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata ggccatatct
    30541 ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt cgccacgcga
    30601 atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa cacgcagtct
    30661 ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga ttggactaac
    30721 gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg tacattgctt
    30781 gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc atccagttct
    30841 tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga tttgatacga
    30901 gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc tgtttctact
    30961 aattggcagt gcgctaaagg cccttccact tggattaatg gttcatttgc aaaaactagt
    31021 tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa atactccaaa
    31081 aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc attgaacgtc
    31141 agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta tccgtggtta
    31201 aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg acctaattcc
    31261 cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa actgtcgtct
    31321 gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc ctacccatta
    31381 taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag cacatgtttt
    31441 acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc ttattggtat
    31501 ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac gaagaaatta
    31561 gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca atcgtagccg
    31621 ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa tcaatggcaa
    31681 aagtttttga agcaataata cgtctttcct tttttgcttc ttatccccaa agaaacggat
    31741 ataacttatt atgtattgaa tttttttgga aaggcgaatt atgacaagat ataccttgcg
    31801 ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa agatacaaag taagaaagca
    31861 gctgtcaata gaatggaaac aaaaatggcg agacatcaat cagtctcgcc ttgtttttta
    31921 ttgtatacat tagatatgga gcaggttacc ataaagatga ttattagtcg ttattaaata
    31981 gaatccttcg tatttttttg ttctatcact acaatatctt tatttggacg tttgaattgt
    32041 attccggcac gttctagctc ctgtttcact cctacatgga ctttgacatc ttctcgtgtc
    32101 atttttaaaa cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt tttcttcgta
    32161 ttgatcatca cgacttgctt tttatcatat gtgtctacca atagcagttg tgttcgatct
    32221 aacgaaaata atttgtaata attaatagcc gtattcccaa aaaagaattc cactacttta
    32281 ggataaaatg tcttaatatt taaattacta ttcacaggtg tttcgattag cttcatctca
    32341 ttcctctttt ctatatagtc gatttagatc caatcttaag ttatgttacc atctttccta
    32401 gcattaagga atcggttaca attgaacctt taaagacaaa aggatttacc ttttatcact
    32461 taatagtata acataatttc gtgagaaaaa aatacaattt tttcttgacc atctcgttac
    32521 gtaaaggatt atgcttacat ttgtcaggag gaaaaataat ggagtacaca ataaagaaaa
    32581 tgtcagagat atcaggagtc agtccgagaa ccttgcgctt ttatgatgag attggactgc
    32641 taaaaccagc taggatcaat tcttcaggtt atcggattta tggcaaaaaa gaagtcgatc
    32701 gtctgcaaca catcttattt tatcgaacaa tggcattcaa gttggatgat atccaagagg
    32761 tcttagacaa tccatctttt gaccaccaga aagctttaat caagcatcag caaatgttgc
    32821 tggaaaaacg ggcacaaatc gataccctct tgacaactgt ccaacagaca cttgatatgt
    32881 acgaaggagg aagaaaaatg agtgacaaag aaaaatttga agggtttaaa caacaaaagt
    32941 tgaaagaaaa tgaagaatcc tatggacaag agattcgaca aaaatacgga aaaaaaaagg
    33001 ttacagcatc caatgaaaaa tggatgaata tggataaaga aacgtatgaa gaaatgcaaa
    33061 ctgttgaaaa gcagatgtta tttgatctca ctctttactt aaaagaacca gaagatgaaa
    33121 gcttagcaga ccgtatcttc caagctcata agaagtggtt ctcttacagc tggcctgaat
    33181 atcagccaaa agcgcataaa ggactaggaa tgatgtacga agccgatgaa cggttcactg
    33241 catattatga cgagcgaagt ggtcatggtg cagcaaaagc tttaaatgaa attatccaaa
    33301 aaaaggcgta atcttttaca tgatccaaca aaaaagtaca cgtaaaatga cctaataaca
    33361 aaacagcaga cacccaagtt agatttttat gctaactttt ggtgtctgct ccattctttc
    33421 acaagttttt tcatcctact ttaaatatat tcatcgttga ataaacgtat aaggtatttt
    33481 cactttatcc tttttttctg taagaaacaa ttgaaatgcc atttctccgc aagcttccag
    33541 atggtggtcg atggttggga aaccaagaag ctcgctgatc agcaaattat cgcgtccaat
    33601 caccactggt gtcttatctt gcagatagct ttgcaagatg cctgctgcaa ccatatccga
    33661 atttgtaaag acaccatcaa ctccaagctc tttaaaaaag aagcctgctt gttttccttt
    33721 ctctgcatct atacaatccc aaaaaacaag tgattcatca aaagatggaa aaagttgatg
    33781 acatagctgc aaagtgattt ttgagttata acttagcctg ccacttcgcc ccaacgtgat
    33841 gcccaattgc tttatacctt gctgcttcaa ataagtaaga ccttctgtaa ttgattgttc
    33901 tcgatcaata aagacacagc ttgcctgttt tcccttgatt tcttcacaaa aaactagagg
    33961 tccatatttt tgatatgcca ataaatcatc tagtgtattg gcacgagacg taatgatcaa
    34021 tccatcgaaa gctttagctg caaattcttc aagatacctt cgctcttttt ctttatgata
    34081 gcccgtcggt aaaagtgtca ctttgtaatc ataatcaaac gctgcttcaa tgatcccact
    34141 gatcaactga tcaaaatacg gatgattggt ataaggcaga atcactccaa tgcttttcgt
    34201 ttttccataa cttaactgtc tggcatttgc attagggata taatcgactt ctttcatgac
    34261 cgcaagtatt tctttccttt tttcctcatc tacatatgga tgtccattga ttacacgtga
    34321 aatagtagaa actgaataac ctgtgagctt tgcgatatct cgaatgtttg ccactgctgc
    34381 ttcgccacct atacaaaaag attttcttgt aaatagtcta ccacagctgg accgcattca
    34441 aaaaactgat acatttttcc ttcatgcgaa aaactgatcc gctgcccgcc ccaatatata
    34501 tcttttgtca aattttcgat ttcattcatt ggtatttctt ttactatatt cctattcaac
    34561 ccttgaaaac tgtacaacgt taatacggat cccttgaact cgacaaacgc ataaaaataa
    34621 ttgttcgttg tgccatctgt tatctctaca taagtattca ttgcaagccc tccttttgat
    34681 actttgagta tagtgtatga aacgcgtctc atagcaagga gtttgttttg ccaaagaaaa
    34741 gttttattcc tctttcatta ttaccaaagt ctcttatttt aaattgaata cattataatg
    34801 atactgtaaa tatatcttta ttattagtga atggagtcaa gtccacaatc ttgtgtaaaa
    34861 gttcaacctc atcaaaagca caatgttttg cgttgtgaac ctaactgcca tgcctttctt
    34921 tctgaggaga gaggtcagga gcagtagcgc agcgactctt gatgtctctc ctcagaaaga
    34981 agagactcgc acaggttcat ggtgctaaat gttattcgaa ttaaattcta aatattttcg
    35041 tggagaagcc agccactcag tgtctttttc catcaaaagt gttccaatta tccgagtgat
    35101 ggaattgata tttggaaaaa tccgaatcac tcgttctctc cggcgaatct cttcgttcaa
    35161 ccgttccaac acattggttg tacgaatcct tcggcggata ttttcaggaa aagacaaaat
    35221 ttgaatagca tcttcaaagc cgttttctaa aatgtcgcaa gcagcactta attttgaatc
    35281 acaaccatat ttttccaaga agtgctcttt tctttcacgt gtcaattcaa gttcagaggc
    35341 tttaaagata ctcttcaact cgtcttttac atcagaagaa actttctttg gcagcttatc
    35401 aaaaatattt ctcaaaaagt gggcttggca tctttgccag ctcgcaccta aaaagttctt
    35461 gcgaacagcc ttaactaacc ctttgtgtgc atcagaaatg accagtttta caccgaacaa
    35521 acctcgttga acgaacgatt gaaaaacggt atcccaatta tcttccgatt cgccgtcttg
    35581 aacatcaaaa ccaagaatcg tacgtcgtcc atcagaatca atgccgatac caatatatac
    35641 accttttgag acaatacgat gattttccct tactttcata tacagggcat cacacatgaa
    35701 aaatggatat tccgagcctt ctaggctccg gtttctccac tcaaaaacaa atggatccaa
    35761 ctgtttcatc agatttgaaa cgaatgattt agaaacagtc gcaccatccg ttagcagttc
    35821 aactgtttta gttactttgc gagtggagac accagaaatg accatttcaa tcatggcagc
    35881 gatcaaagct ttttcgcttc gctgatatct ttcaaagata gtaggactaa acttaccgtc
    35941 tctggttcgt ggaacatgaa gttctaacgt accaattcga gttgtaaacg aacgatcgta
    36001 gtaaccattt ctttgtgatt gccgttcttc tgaacgttca taggcatttg cctgaatata
    36061 ttgatttcgt tgttcttcca tcagctgatt aaacatgata gtcataagtg tttttgcggt
    36121 atcattcgca ccgctttcgt ttataattgc ttgaatttct tcgctttcaa aagtaaaatg
    36181 tacttgagtc attgatatca gtcctattcg agtatgtttt ctgcaaaaaa cattgtactc
    36241 ctaactgaga tcaatggctt ttttgatgct tcacttttac acaattatat ggacgtaatc
    36301 gtgaatggat atttactttt aatagataaa atattataac tattaaaaat aactattttc
    36361 attataatta cacacaaaaa agagcgtggg acaaaagtaa agaacacttt tgtctcacgc
    36421 tctaaacacg tataaacagc ggaagcagaa gcaactcctt cggaaataag ctgaaattca
    36481 caaaaatttg aaaagcaatt tccgtgaatt tcttcttatt tcttggagtt aaacgcttct
    36541 gttccagcct ctttttatct gtctttcact aatgatcagt tgtagataag caagttgctt
    36601 tttcttacat cataccgccc atcattgatg gatccattgc aggagctgct ggcgctgctg
    36661 gttctggttt atctgcaacg actgcttctg tcgttaataa caaggcagaa acggaagcgg
    36721 cattttgcaa ggcagaacgt gttactttag ttgggtctac gatacctgct tcaaccatgt
    36781 ttacccattc tccagtagca gcattgaaac cagtgcctag ttccacgttc ttcaatttat
    36841 caacaatcac tgatccttca taaccagcgt tttcagcgat ttgacgaatt ggttcttcta
    36901 aagcacgaac aacgatcttg atacctgttg ccacgtctcc ttctgcttct acagcagaaa
    36961 ctttgctgat aacgttcact agagctgtac caccacctga gaccattcct tcttctactg
    37021 cggcacgtgt agcattcaat gcatcttcaa tgcgtaattt taattctttc aattctgttt
    37081 cagtagctgc accaacttta acaacagcta caccacctgc taatttagct aagcgttctt
    37141 gtaatttttc gcggtcaaaa tcagaagttg tttcagcaat ttggtttttg atcaattgca
    37201 cacgagcttc gattgcttct ttttctccag aaccttctac gatcgttgta ttgtctttat
    37261 ctacaacaac tttagaagca tttcctaagt tttcgattgt tacatctttc aattctaaac
    37321 ctagatcgtc agtaattact gtaccacctg tcaaaatagc gatatcttca agcattgctt
    37381 tacgacgatc gccgaaacca ggagctttta cagctactac gttgaatgtt ccacggattt
    37441 tgttcaatac aagagttggc aatgcttcac catctacatc gtcagcaata atcaacaatg
    37501 gacgtgattg ttgcaagatt tgttctaaca atggcagaat atcttggatg ttagagattt
    37561 ttttgtctgt gatcaagata tatgggtttt ctagaacagc ttccattttg tcgttatctg
    37621 ttaccatata ttgtgataag taaccacgat caaactgcat accttctaca acatctagtt
    37681 ctgtttcgat cccttttgat tcttcgattg tgatgacacc gtcgtttcca actttttcca
    37741 ttgcatcagc aatcaaatgt ccaactttat cagaacccga agaaacagct gctacttgtg
    37801 caatggcttc ttttgaatca acaacagttg agatattatg caattcttcg actgctgctt
    37861 ttgttgccaa ttcgatcccg cgacgaatgc ctagaggatt agcacctgca gttacgtttt
    37921 ttaaaccttc gcgaacgatt gcttgtgtca gaacagttgc tgtcgttgta ccgtctcctg
    37981 caatatcatt tgtttttgat gctacttctg atacaagttt tgcacccatg ttttcaaaat
    38041 gatcttctaa ttcgatttct tttgcgatcg tcacaccatc atttgtgatc aatggagagc
    38101 cgtaagattt ttctagtacg acatttcggc ctttaggtcc taatgttact ttcactgtat
    38161 ctgctaattt atctaccccg cgtaacatag ctgcgcgtgc gtcttctgca aatttcaatt
    38221 cttttgccat gattgattca cctcattgat tatttttgat tattttttta tcttccaaat
    38281 ttactcaaca atcgccatga tatcttttcc tgcgacgatc agatattctt ttccttcgta
    38341 ttttacttct gtaccggcgt atttttcgaa cataacttgg tcgcctgctt ttacagcggc
    38401 aggaactttt tcaccgtttt ctaacaaacg gccttcaccg actgcaacaa ctgtcccagt
    38461 ttggggtttc tctttagcag cagaagctaa aacgattcct ccgacagttt tttcttcttc
    38521 ttttgcgact tcgataatga cgcgatcacc taatggtttt aacacgataa atccctccga
    38581 taactctttt tttaatctgt tagcactctt aaccttcgag tgctaaccta catttttaat
    38641 aatactcatt ctccctcttt tttgcaagct tttaaatcaa agaaaagaaa ttttattctg
    38701 acagctgctt attgaaatta tgatataatt gaaatcaatc gatataagct tgccttgcat
    38761 gcttgttatc ccttaaaagg aggaattaca tgtctttaaa aaaatatagc ttcctcagca
    38821 ttttttgcta tggtctaatt tttatctcac ccttgatcct agcttcgata ggacttgtaa
    38881 aatctacgtc tgaattgatc acagcaactg cagtcatgta tatactaggc gccgttgtcc
    38941 tagctatttt ttattttaag caacgtgaac ccttagcgat cgaatcagcg gtaaaaaaag
    39001 cttctgctcc aaagattgtt atttatggat tggttggaat ctttgtcgca ttgattctgc
    39061 aaagcatcgc tgtcatgtta gaatctgtat tgtttggcaa agcaacccct tccgagaata
    39121 cacaaaatat catccagatg atcatggaag cacccgcttt tattttggcg acaactgttg
    39181 ctggacccat catggaagag ttcgtattcc gccgcagtat actggggatc atcagtcgat
    39241 attcaaattt ttgggtcgga gcagtgatca gctctttgct gtttgcgttt gctcataatg
    39301 atggtcatct gctaatctac tttttcctag gtttcttctt cagcctagag tacaaagcaa
    39361 ctggtcggat ctggacatcg atgatcacac acgtaggaat gaacacatta gttgttcttg
    39421 ttcaattagc tgtccaaaaa gggctagtat aaacatgact ataaatactc ctgtcatcct
    39481 agattggtga ttttcccaat ctaaagcgac aggagtattt ttgtttttta gtcgttcatc
    39541 ttttctgctg ctccatcatt ttttgtctgt ctttcctttt tgcagtttgc agaatctatt
    39601 tttttcaaaa atgggaaata aatgaaaaaa ctaattgaca acacgatacc ttgtaaaatc
    39661 gctgcaggca ctccgcctac taagaaaccc gagaggatcg gcggtgttgt ccaagggact
    39721 tggactgctg taaataaagg aacgaatcca aaatataaag cggaataggt aatcaatcct
    39781 gaaacgaccg gtgcgaaaat aaaaggaaca gccatcatcg ggttcatcac gatcggtgtg
    39841 gaaaaaataa ttgattcatt gatgttgaaa aaggctggta aaagagcaag atttcctaat
    39901 tctctgtatt tcttcgattt cgcaaaaacc gtcataaaaa atacagcccc taacgtcaat
    39961 ccagcacccg taaccgtcat gaactgatcg agaaattgct gagtaacgat atgtccgcca
    40021 tttgccaccg tcaactcttg tcctgaggct aagatttctg cattcgctaa tgaattcgct
    40081 tgcaagacgg gactaaccaa cccattgacc atcgcagatc catgaagacc aaataaccat
    40141 aaaaacggaa caaaaaaacc taataaaagc acaccaaaca acgtgtctat gaacgattga
    40201 atgggaagct ggacatatgt ccatactcct tgaacaacag tcattctgaa caactgttga
    40261 aaaaccgctg aaccgataag cgtaaaagtg actataacaa tgattggaat caatgaagta
    40321 aaagactgcc aaacattttc aggtatcttc tctctttgct tccacccttt cgcaagtctt
    40381 actaaacgtg catagcccca tccgatgatg agcccagttg taactgctcc tagtatccct
    40441 tcagcacctg tccagacagt atctatctga gtatttagtg gctgaataag cagtagataa
    40501 gcagacaagg ctgttatgcc gactggtaag cgatcgatgt cttcttcttt tgcataagcg
    40561 tatgcaatgg caaaaacgat ccaaaaggct atcacatcaa atgtagcctg gtatacttgt
    40621 gtaaaataca tagaaaatcc tgttttgtct atccactgag cgatgtaatc cacaggaaaa
    40681 tgagcaaata agaaaaacag cgagccaaca ataattattg ggatcgtgta agtcattcca
    40741 ttacgcaaag cagttaaggg tctaagattc acaacagatg ttatcattgg taaaaaggca
    40801 cgatctaacc actctttcat ttcttatctc cttcaacttc tatttttatc aatcagtttt
    40861 cttaatttaa ctatacccaa tgatcacgaa ccatgaaagg aatgcgcact gagcttttca
    40921 aaaatcggat tctttgacga atattcagca actgcttaca atggacagga ataaaaaagg
    40981 aggtttttta tgaaaattgc actcattgga gctactggcc atgcaggttc atttatttta
    41041 gacgaagcat tgtccagaga tttagacgtg acggcaatcg tgcgtcatcc agaaaaattg
    41101 ccatcagatg ttccgtttat acaaaaggat ttatttgagt tgacaactac tgatcttgca
    41161 tcttttgatg ttgtcgttga tgctttcaac gctcccaaag gaaaagaaga aatgcatcaa
    41221 acttctttgt cccatttgat tgagatgctt gaaggaaccg atacacgctt aattgtcgtt
    41281 gggggtgcct cttcgttatt cttagataca gaaaagacga ctcgtatgat tgaccaagta
    41341 cctaaaaatg cacccttcta tccaacagct ttcaatatgt acgaaggatt gttaaaattg
    41401 aaacaatcta aaaatctaca ttggacttat atcagtccag cttctttatt tgatccacat
    41461 ggcaaacgta caggtactta tttgttaagt gatgattatt tacaaagaaa cgctgctggc
    41521 gacagtacga tcagcatggc agattacgcg attgcattag tagatgagat actggatgag
    41581 aaacatgaaa agcagcacat cagtgtagtc agtcgataat acggttattt ttttaatatg
    41641 ctaaaaaaat aaaaatagaa gataaaacca accctgattg attttatctt ctgcttttta
    41701 tatttttatt gattgaactg tttaaatcga aaatagctca gttgcttcat ctggatctgt
    41761 atcatagatt tcaaaatcat gtccaacgcc aaaatctttt tctttcaaaa cattttccat
    41821 tgttaaatga gcaagttctt tcatattgtt ttccttgcta agatggccaa gatagattcg
    41881 tttggtccga ttaccgatca catcagtcat gacaagtgca ccgtcttcgt tggaaagatg
    41941 tcctctatct cctaaaatac gctgttttaa attccacgga taaggtccca tgcgtaacat
    42001 ctcaagatca tgattacttt ccatcaaata accatcggca ttttcaatga cacctcgcat
    42061 ataatcactg caatatccgg tgtccgtcaa catgacgaat gaccgatttt ctttatggaa
    42121 acgataaaat tgcggagccg ctgcgtcatg ggagacgccg aaactctcaa tatcgatatc
    42181 gccgaatgtt ctgactttcc ccatctcaaa tagatgtttt tgttccactg gtacatttcc
    42241 aatcagtggt tccatcgctt cccaagtctg ctgattggca aaaacatcca aatgatactt
    42301 acgagccaag acacccacgc catgtatatg atctcgatgt tcatgcgtca caagtattcc
    42361 atctaattgg tccggtgtcc gatcaatttg agccatcaag gaagtgattt ttttaccgct
    42421 aagtcctgca tcgaccaaaa gccgtttttt ttcactttcg atataaagtg aattacccga
    42481 acttccactt gctaggatac tgattttaaa agctggtttt tcttcagcca tttgactcct
    42541 acttcccttc ttactttgta aacttttttc attatactcg cttttttatt gatcttccac
    42601 ttttggtaca gtattgtttg tcacgatcgt attgctgaaa gcgttgacac tctcaatttg
    42661 gaccacttta tcgcttgttt cgatcgctac atgccaaaca ggaacataca catttttttc
    42721 ccggactttc agaatacagg aataagctag ctgacgccat aaaatttttg atcctcttgg
    42781 tattttgtta ttgatgtaca atgtattgat tgcttcctct tccgtataca gttccgtctt
    42841 ttcgcgcagc tgctcgatat cagataaatg cgtctgcatg tatttcgtga tgctataatc
    42901 attatcgttt ttttctaaag aaagaacgat ttttgctgta tcatcattga atgggatgcc
    42961 ttcatactct tggcttgctt ggatttctag tggttcttct tctaaatgag aaaagttttt
    43021 cgtgtacaca taatcattac cgaataaaat attgtctttg ttagataaaa aatcttccaa
    43081 ggaactacta attttatctg gatcgatcac gttcttttca gcatctgaac tggataaaga
    43141 tttcgtcaaa acatcaccgt ctaaaaacac accacttgcc agcaagccgc ttttaccagc
    43201 cttgcgataa ttcgctaatg aagtgctaag attggtctgc tccgcgctaa gataatagcc
    43261 ttgcttgttc tcagacgaaa tcttccccgt gtaagtgata tcgtcgctag ccagacgttg
    43321 ttggattgat tcagtctgat tagagcgaat gacattgttt tcttggtata ccgcgtcatg
    43381 ataactgctg aataaaaaca cattcaaccc taaaaaagcg acgaaaaata tccattcgat
    43441 ttttttgaaa tccaattaat tcgcctccaa tccaggtaat ttttccagaa ggtcgttagc
    43501 tgaataccag ttatctttat atttcacata ccattcaggt aagagatcaa cgacacgatt
    43561 cgtttctttg acattttgcc atgtatagcc aacgatcact gaaccaatct tgttgctatc
    43621 tgctccctta gcttttaact gctggatgat ttcattcgta cttgcaagtt ctacttcttc
    43681 gtctgaaggt atcggcactt gaatcgtatt caaactggtt tggatattca cttgcacaga
    43741 accattcgtt tcaccgccaa cttccacacc aatctgtcct ctgctgtctg tcccaaaaac
    43801 cgggaagcct tccacgaata tccgataatc gatcgtattg tcactgcggt caaaatatct
    43861 gaggttccct aaagaactgc ctaatttgct aatatagggg aaactttcag aaaatacact
    43921 agcattttct tttttctctg gcagctctcc acgaaaatca actacttgcg tttccgaatg
    43981 gatagacatt gtttcattcc caccgtaaaa aatcaattca ttgctttcat cattattttt
    44041 cacgctatct ggtttctgga aaaaagcatt tctaaaaagt gtatagggtt gctgtgacag
    44101 gatataacta tactttttca gtttgatcga atgtttcgta gcatattgcg tgtcgatcgt
    44161 cgatggttct gttgtcattt ctgtccattt tgtgtctttt gctgttaatt ctttattgat
    44221 atctgtccaa ttcaacgaaa tatttgcttc atatacgaga tggttcgaat aattcaaaaa
    44281 tcggattttt ttatttcctt gatcgaattg tattctagta aaatacatat tttcgctttc
    44341 ttttgccaag ttcgacacat ctaaatctaa atcaaatgct tccttgtatt ctcttagcaa
    44401 aaaaggcgca ttataggcca actcaaaacc agagttcaga tttttcacct gttcaaactg
    44461 tgatttgtct cctgaaacta cttcagaaag ctttccaaat cttgctttat cgatgatcaa
    44521 ttgcaatcgg tacacgagat tttcactgtt ggtttcttgg atcattcccg aatggtgcca
    44581 tgtaacatga agcggaagaa agacttcgga agtttttcga ttgctttgag actcgatcat
    44641 ttgagtgttt gtctcatcta cgttgattga actttttcct gctgggctca gccagattgc
    44701 ataagagaag tatatgctta acgcaaccat caaaatgagt cctatacgaa cgactttttc
    44761 agagattctc attcccacca ttcctcctca taaggttcat atggcagcga gatataaaaa
    44821 gtcgaccctt ttccttcctt gctctctgcc caaatagcgc ctcgatgcgc cttaatcact
    44881 tctctagaaa tagctaatcc aagtcctgtc ccaccttgtg ctcttgcacg tgctttatct
    44941 actcgataga accgctcgaa cactcggttc agatcttttt taggaatccc caatccttga
    45001 tcggtgatac ttaaaaccac gttattatgc gtttctaata agcgacaagt aatcgttccc
    45061 ccatctggtg aatacttgat cgcgttattc atgatattat cgactacttg gatgatttta
    45121 tctgtatcta cttcgaccca aagatctctt tgcgtaaatt cacgacggat cttataattt
    45181 ttttcctgat ttcccaccat catatcaaac cggtcaagaa caaaattcac taactcgttg
    45241 aaattgacat attctaattg aagctgggta ttacctgtat ccatgcgaga tagatttaag
    45301 aggtcattga tcattcgaat cattcggtct gtttcatcta gcgttacctt caaaaaattt
    45361 ggtgctactt cctcgtcttt ccacgcacct tcgctcaatg cttcgatata actacgcata
    45421 cttgtcaacg gtgtacgaag ttcatgggaa acattagaga cgaattcacg acgttcccgt
    45481 tctgtttttt cttgttctgt cacatcatgc aagacagcta ctaatccgct gataaaacct
    45541 gattcgcgac ggatcataga aaagtctact cgcaagatca tctgatcact ttccaatgta
    45601 ctggttgaac ggtcaagtac catttcgttt ggatcttcta ataatttccg caacgtatat
    45661 tcttcttcga tatctaaaag tgtcaaaatc gattgtccga ttgcttcctc gtttttgata
    45721 tctagcaagg aaacagccat atcattgatc gtaatgactt tacccctgcg atctgtcgca
    45781 atgacgccgt ctgtcatatg ggatagcacg ctatccaaac gattgcgttc agcttccatt
    45841 gtatcttgag cttcttcaat acgttcagct aactgattga aggtatcagc cagctgtcca
    45901 agttcatctt gtccatgcac tttgactttc cgactgtagt ctcctctagc gattcgaatg
    45961 gcttgttctc tcatttcacc gatcggctgt gtaatcgaac gagcaactaa aacagatacg
    46021 atcattgaaa taaatgccgc aattagtgag gcagtaaaga aaatgactgc tgtgttattg
    46081 atttccgaat atttttgttc gatattactt ttgacgtaaa gagccccgat aacggcatcg
    46141 ccggtcgggg acaggatcgg ttgaacattg atattcaccc gtttatcgtc gttatctaaa
    46201 gcaacataac gtttagtcgt gaaatcattg atatcaacat aatcattttt ctttcccaca
    46261 gcgctttgtt cattgacatc ggttgttcct cggatgatcc ctttttcgtc aacgacccgt
    46321 atttcgatga tatcgctagt cgcactgttt tcaataaatc ttttgatatt cgcatttacc
    46381 tcttcatcat ctgcattatc ttttcgattc aattccgggc tgattgttgt tgctaatgat
    46441 tccaccgttt gattcatatc tttaataaat gtattgatcg tggaacgttc taaaccacgt
    46501 ataaagtagg caccaataat ttcgatcgag atcaataaaa tcagaataaa ggtaatagcg
    46561 atcttaaaat tgactgatcg aaaaaagcgg acttttcttt tcatccaatt actcctgttc
    46621 tgggttacgc aaatagtaac cgacaccacg acgtgtcact aaataatttg gatggcttgg
    46681 attatcttcg atcttttcac gcaaacgacg tacagttaca tccactgttc gtacatctcc
    46741 aaaataatca taaccccaaa ccgtttgtaa taaatgttct cttgtcatca cttgtccaag
    46801 gtgcttcgcc aagtaaaaga gtagttcgaa ttcacgatgt gttaactcga ttgtttctcc
    46861 tcgtttcgtc accatgtaag cttctggatg gatcgtcaaa tcaccaatcg tcaattccgc
    46921 aggtgctgcc tcttctggtt ccttcgcagc cgtggcaccg cgacgaagat ttgctttgac
    46981 acgtgcaact aattctcggt tggaaaatgg cttggtcacg taatcatcgg cgcctaactc
    47041 taagcctagg actttatcaa tttcagaatc tttggctgtt accataatga tcggcatatc
    47101 ataagttttg cgtacctcac gagcaacttc taagccgtcc attttaggta gcatcaaatc
    47161 aagtaaaatc aaatcaggtt ctacctcagc aactttttcc aaggcttctt ccccatcata
    47221 tgcagtataa acatcatagc cttctttggc taaattgaat ttgacgatat ccgaaatcgg
    47281 cttctcatca tcgacaacta aaactttttt cataaagagc cacctcttat atattattgt
    47341 cataccttct cattataccg tatttctgct tttgtttcat ctttgcctat aaaacaaaag
    47401 agaaaattaa aatgaaagtg caaaatactt tattattttt tgttctaact tcaataaaaa
    47461 aggaaaacac tgattttcac ctcagtgttt cctttttttg atttatcctt ctaaaacttg
    47521 tacaccgaca cccaataatc ccggaccagt gtggactact aatgccggag aaatctggcc
    47581 aaagtagatt tggtttgctt gtgggaactc tcttttaagt ctcgcttcca tttcaattgc
    47641 ttcttctttc gcatccccat gggcaacagc taagttgaag acctttgcat taccgatttt
    47701 ttctttgacg taagcaaccg ttttatccaa acttttttta cgtcccctag cttttcctac
    47761 tgtgtaataa atcccttctt cattacatga gatgattggg ttaagcttta aagcggttcc
    47821 tacaatagaa gcaactaaac cgatccgtcc acctttttga agatattcca atgtcgcaac
    47881 attaaagaaa acttttgttc tttcgactaa ctcattcaga ttactgatca gttgatccca
    47941 ttctactcct tcttccagcc attttgctgc ttggatcgca gaaaaacctg caccaatacc
    48001 aatatttttc gtatctagta catatgcttc caattcttct tgctgttgtg caattaaacg
    48061 aaccacatta tatgtcccgc tcaatccact ggaaattgtt accgcaagta ccttttcata
    48121 accgtctttt ttgatttgct caaagatttc tgtgatcgac tcaccatccg gcaaagatgt
    48181 accagggatt tcctctggca aacgttgata gatctcttca gaggtaatgt caattttatc
    48241 tgtatatacc tgatctttat aaataatttt caatgggatc atatacatct cgtatttttt
    48301 tatcaattct tctggcacat ccgtacctga atctactaaa attgcgattt tattgttttt
    48361 catttgtttt ctcctctttt aaatattcgg tttctagaac aatcgttttt tccgcaagaa
    48421 gtttcattgc aaacgataaa gttgccgctc ttacagcaat atattctaca ggggttgcct
    48481 ggtcgaattt ttgcggatga gatttacctt gtgctaattg gctgaccatc cacacagctg
    48541 cttcttgttc atcgcaaaat agattatagg cttcacgaat tcctactgtt tttccttgga
    48601 acaatatccc ttctttgatt tcaggaatag tcaatacttg tttcaacgta gtgattgcaa
    48661 gtaaaaatgc tacgtgttct ttgttatatc tttttttaac tggtgctggt atcaaaccta
    48721 gtttaacgta attgtttacc atagaagagg taagcaatgg atgcttttct ccttgtatca
    48781 caggtgaaag ataacgatca actaacgttc tgacttggtc catataaagt tctaagtcag
    48841 ggagttcttc ccagtgaggc agacggaatg tttctagttc cttcccccac tccattaatt
    48901 gctgacgcat tttttccatc ataaccgctc cttttttcat ttcataaaca tgatatcaga
    48961 cttatgataa acattcaatc tagttgcttt aactaggtga tttgattagc gggattccat
    49021 gaaaacaaaa aacaagaagg ctaagccttc ttgtctttta tcgttaagaa caggagaccc
    49081 gccggggaga cggcgggaaa ggagtaaaaa atgaaaaaag tgttagggtt tgttctcttg
    49141 caagaacttt gtttgttggt atgcttatat aatagcgagc aaatgtgaat aatctatgaa
    49201 tgatttccta taaggaagta aagatttttc tgattttctt taaatgtagt cttgcatttt
    49261 aaaattctta gtgctactat tattaagtac tttttgtacg ggccgttagc tcagttggta
    49321 gagcagctga ctcttaatca gcgggtcgcg ggttcgagcc cctcacggcc cattgggtgc
    49381 caaacccacg tcaccagtac attcttggct acttaggtag tttcgaaaag agtgtacgtg
    49441 gtggcatttt ttatttttcg ggtatgaaat tcgggtatgc attttagtat tttatttttt
    49501 aataacatcc taatcagtca ataaacctgt agattatcac agtatgtact tcccctccat
    49561 tttgagggga gatatcattg tagaatattc aaataaaaat tatttagcaa caaaatacta
    49621 ttttgttaaa gaaatttaaa ctaaaaaatg catctatttt cctgtatgtt cagtcacgat
    49681 ttcttgtgat ctgtttgctg tgtcttctac atcgcttttg gctttttcca gtttttcaat
    49741 ataattattc aaattactgt tttctgattg cgctttttct agtttttgtt cagtactttg
    49801 caattggttt ttaatagtat ttttttcatt tgacaattga ttaatttgtt gatttaaact
    49861 attaacagta ttttgagcat cattaaattt attttgttct gtttgtaaac gattattcca
    49921 atcagtgttt aattgattta tttcagctat tttttgattt atttcatcaa ttttagcatt
    49981 gatttcattt tgtttacctt gcaattcagc attttttcct gataattgat tctctaaatt
    50041 tgttttctct atagttaatt gatttttatc attttccaaa gtcaacactt tttgtttata
    50101 ttcttcaacg ttagcctttg cttgctcaag ttcttgatta tttgaagata atcgtgaatt
    50161 taaactacta atattttgct ctttattttc tagtgcttta tctaatgact ccaaattatg
    50221 attgatttgt tcaatattat cttctccacc ccataacaaa atgtcatcgg caaagttttt
    50281 ccctgcgatc cctgctaaca ctaatccacc tataattaat ccacttacta caattttctt
    50341 attcatatca tcattctcct taacataaac tgaactaatt atagttaatg atttcacaat
    50401 aagtcaattt aaatatatat ttttttattt tatttttata aatcttttcc ttaattcgaa
    50461 taaagtcaga tgtaactgca tacaaacatt tgagagtata aaatattttg tgtaaataga
    50521 aaaaaggaag tcccttctgt agaataaagt taccacaccc attcacagaa aagaggactt
    50581 ccctatgaac gattttatta cagaactcct gcagactcta gccaccaaag gtgatttgaa
    50641 cgagttattc cgctcccatc tggagctggc cgtaaacacg ctactgcgga cggagctgac
    50701 ggccttcctg gattacgaga agtacgaccg gacggttatg agccgtctgc tctgaccaac
    50761 tgagctaagg gtacggaaag ccatcacaaa tactgcaaac aaaaagaatg gactagacta
    50821 gataataatg gatttcattc tttgttgtgg tggctaattt attattacat aaatattatt
    50881 gaaaaaacta gacttttttg catattaata aaaaaagaga caccatatag atgtctctca
    50941 actatgtttc tatgtgggcg catacgtccc gatgtgtaca ttaaaaatag gcgcttacgt
    51001 ccttctataa ctacatggta actaacgaac agaaaaatgt caacaaaaat atcgtttact
    51061 ttgtaaaata tttttttaaa ctttcatcta cttcattgag gacatttcca ggtgccttca
    51121 tatttccaga aggatcaaac ttgttaattc ttctaattct atttttacta atcgtttgga
    51181 tattaagtgg cattacatag gattcttgtg tatatttctt gtagcaatta aacaccttct
    51241 gatattttac catatcttca gataatgatt ttatttcttt aattacttgt tcccttgtct
    51301 cgcaaaccaa attatgtttt acagctcgtt tttctacttc ttccactgaa agcttttcac
    51361 ctttagcttc aatatcttta ataaaactat tcaaatcgct ttcatcaatg atgcctctat
    51421 ttaatgaaag taaaataatt atcctaagtt tctcttttaa tttatcagct tggcttataa
    51481 attgtttaat actagcaact tctaatattt ttcctacaga tacataatta cttttctttt
    51541 ttgaagagat tggtactaca catacaacac catttttcga attatcttta ttatttaaaa
    51601 caatagcaaa atgattacca gataactcat aacccacgtt caccccaaaa tcaacatata
    51661 caatagttcc tcttttataa accctataat ttttattagg catgcatctt tctttattaa
    51721 gataaaaact tttactttgt aaccagttgt gtaagtattt atacttagga tatccagata
    51781 aagcttctcc aataaaccta acatttgctt ggttacatgc tgcttctatt tcctcttttt
    51841 taactataat taagcacctc ttctcactaa ctattaaatt accgcatttt tacaagataa
    51901 tcaataagtt atggacaaat ttgcattatg tacttcccct caaaaacgag gggctatttt
    51961 tatcgttgcg gaatatttaa ataccagcgt ttatcatgaa aatcttgtgc tccgccttta
    52021 gtgttccctt ctggatcatt cgttgcccgc atcattacat agactttctt attagggaag
    52081 ttgcgcatgt taaaagatac atgatagcca acatttccta aagtattata agcttgattt
    52141 acatctggtc tatatattcc atcagctttt actcgagcta attctttccc agtattgtaa
    52201 tccataatga agatatactc gtatttatag ttagcgatgt gccaaccagc cacatgtaag
    52261 tttgcgtttt cgatttctcc gaactgatca atgtgggcgt aatttgttcc atctgtcagg
    52321 gtaggatttg cagcacctgc tcgagttgga tcaatgactg gtttatcatc tgaagttgtc
    52381 ggattttcat cggtaaatcc atgagccaaa tcataggcta atttttcttt acttacgccc
    52441 atttcagaaa gataaccgta aggatctgta tgatcgcccc aaatattttg tgttacccat
    52501 aaatgcgatt tgatccctgg ttgattatac ggtgtgtcta gtgtaagtgg aataccatat
    52561 ttcattgctg aatctctagc taattcaacg tatgccttgt agtttttctc aaacgttgct
    52621 ttatcatgtg tgtgttgtaa ctcaatctgc acaggactat ttgcattagc atacgaacca
    52681 gcaccgtact gtacataacc aggttgaccg acttgataaa caattccgcc gtctcccaca
    52741 atataagcag tataagcgct agtccatgaa cgttgcatat actgcgcttc attgcgtcct
    52801 gttgctgttt cattagccgt ttcatgcagt aaaatgtact gattatttgc tacttgagaa
    52861 ctaccttcgt ttacacctaa attaaattca ttgttaatcg tataggcaaa cccattaatc
    52921 ggcaataaaa aaagagccgt taataggctc atcgcagtaa tagtaatttt ctttttcatt
    52981 tgtttcctcc tattttttca aattataagc cgacacacca gtgataacac ctaaaaatgt
    53041 tgctgctgca ttgatagtca gaactgccat atctgttcca ttccatccat atgctttgcc
    53101 taatgtggct actaatacag ataaagctgg caatactgtt aaaactgtcc atttaatgac
    53161 ttgataatac ttatcgggta aaatcatttc ttcttaactc ctttcttttt taccgagacc
    53221 tttctctaaa taaagtttta atttgttgtg tgtgttctac caatttttct gcatgtgtat
    53281 ctaatctttc atcgtgtttc tttagttctt catgaatcat caatcgatct gatttgctcg
    53341 attctaaatc tttagtcagc aaatctaaat tgtgacttac ttttgaaaga gtttcagtaa
    53401 tcttcgagaa agatgcagtg actggtctta ttactaataa aatcaaagaa acaatcgcag
    53461 taattgatcc tgcgattgtt ccccattccc ctaaattgat catgtgacaa ctcctttacc
    53521 ttaaataaaa acgcatcact taagatgcgc tttcttcttt gctaatgatt ttatctgctt
    53581 cttcgtctgt aatgcatagt ggaacgaata gtcgaacttg atcgtcagta aaacagcccc
    53641 aatcatacat cattttcaca tcgctaaaac taaacatact actcacctcc ctttgaagct
    53701 ggatttagtt gctctttaat ttctgaaatg tctttgctat tttgtaacga agcaagcatc
    53761 atttttgaat tgatttgtgc taaactatcc gctttttctt tcaatgcagt attttcctgt
    53821 ttaattgcta catcgcttag catgagtttg gcattgatct gttttaaatc gccgttctca
    53881 ttttctaacg actcatacat tgctttgaga ttgtttaaat cgttgtgatc tagtgcgttc
    53941 gctaaaataa tccattgatt caatttagga tcaaacattt gatcagcgat tgttaacggt
    54001 tcgccatcag cacgaattcc ttcaagcggt ggctgatctg tgtaaggaac ggatacaagc
    54061 atgtcgtcca atacttttcc tgcgtactct ccgccagtac gtccatattt ccaaatgttt
    54121 ttcatttatt tccctcccag taattgaatt tcggtttcca atttagaatc ggtggttcga
    54181 cttctgtgca ttcttccggt aaatgttctt catcattcac aatgatttgc tcgaatccgt
    54241 aaggttcaat tggtctatat gctgccttca tatcgattca cgcttttcta aatcgtgtaa
    54301 gtaattgcaa aggtataatc cgatccataa cttgagtttc ttcgccattt aatggctcca
    54361 tctgcaccaa tagataactg agcactgttc aaagtagaac ggtctatcga gccaaccagt
    54421 tgctcaaaac taattggtgg ccgatagcct tctggaattg ttagtatcgt tgaatcattt
    54481 ccaccactgc tttttccgtt taaagccaca aaatatatag aaactgtttt tccttcacga
    54541 taaagctttg ctgatccggt attcccgttt gtaactgtta atgtggtagt agctgtatca
    54601 ttaatgcgtt catcgatctt attgtctaat tcatctatag cagtcgcatt agcattcgct
    54661 tttgtttgag catccttagc tgtggtgtct acttcattaa ttgaagcagt caactgcgaa
    54721 ttaatctccg atactttccc atcggtataa ttgtttgctt taccagtaat ttcagaaatt
    54781 ttaatatctg tggccaaatt atcttcgaca tattctggtg ctagatccca aacataatct
    54841 tttggattgt ttgaatcacg cataccagta ccacgatatt tatactcact aatattcggg
    54901 gttcgagtat tgcctttttc gatcttgagc cagtcaattt gacatgcacc tgttgttgat
    54961 tggggatact ggataatagt taaccgtttg gggtagttaa ctccaacctt ctgcggtgta
    55021 aaagtcagac cccatgtatc aactaaccct tctactggct ctaagttacc atatctataa
    55081 tctccactat cctcgttgta aacaatgaac gtttggatag gtggtttggt tgccttcatg
    55141 gtaattgtat aggtttgacc taaaacaaat tcttcttcca tgttagcttg gtataggctg
    55201 taggcgctag atttgattgg aaacgtaaca gacttattag caatattctc acccaaaggc
    55261 gctttaccta gccagtaagg gtcatcaagt aagtttggct gatatggggt ggctgttgag
    55321 ccttcttcaa tcttaatttt tcttagtcta actttaccgg tcggtttaag tgttcggatg
    55381 tctctaaaca aagggtacca ttcatttggt acgtcagagg tgtattttac cgttcgcgtt
    55441 ccgataagcg tctgccacgt attcaattca tttttaggta ctgtctctgt ctgtaaaaca
    55501 acaaccccgc ctggcaaata tgcgtgttgc agacgtattc ctgaagggtc gccagtgaac
    55561 ccatcttcta acataatttc acaactaatc gtatattgtt tcccttctgc taccggcact
    55621 tgcgctttgc tagcgtactt cgatagttga tttgtgccat ctaaggtaaa cacaatctca
    55681 tcattatcat ccgcgactgt tgcgccagta cctaaagaaa aacttgaagc attaagttta
    55741 gcgctaaggt tcggattccc actataatca tagtccccga agtcgatgct gttactgtac
    55801 atctttttca gcttgccgag atcgccgatt tgctgattgg tttgatcaat acgatcattt
    55861 gtcttatcaa tacgatcatt tacttgctta tctgattcat ttatcaatct tactaattcg
    55921 ttatatttcg agtagatttg atcgtattgg acctgtttat cactaatgaa ctgattgaaa
    55981 gtggtttgca tttcactctg taatttttcc aatgaagaaa tatagtattt cgcttgctcc
    56041 gaattaatat ctactcgttc taacacgtct atgatgaagt tctgaaaagt tacttttttg
    56101 ccgttaggat ctacatattc aaaatatgct tgtttgaatt gatgactcgt gctgaaattt
    56161 gacttagtga acgtgtaact aattaaccca ttagtcgaat cgattatttc tggttctccc
    56221 tcggtatagt taccgtttga aacttcgccc acaaatttca aagtaccgtt tagattaatt
    56281 acaaaaggtg taatttcatc ttcctcaaga agttgtacat tgattgttgt gagcccccca
    56341 tcaccaactc taccaacaac acggtgacgt agataaggct gtctcttatt tgcagataat
    56401 ttaatttcta aatttgccac tcttccatac ctcctaacta aaaagaaact atatacctaa
    56461 gcaccatatt tgcgttatct ccacttgcat tgcttgctgc acctgtaaat atattagtat
    56521 tcgaattata gtttattcgt ttactaaacc actttttgtg atagtcatca aatcctgaaa
    56581 caatttctcc taaaacatta gtaaaagtta cacgctcttt ataaaaaggc gtagttacat
    56641 atctatcccc aattgcttgc ccgttttcgt atgggagcca tactaataaa aaacctgaaa
    56701 ctgtctgcca catttttttt gaaggttgga cagtttgagt atcatgcatt aacgcagtcc
    56761 cactccacaa aatttctcct ttagagagag tcagctcgta actatccgat atctttgaga
    56821 ttgaaacagt tgatccagta gaagtaactg agcatagtgg taaattatag accttatcac
    56881 cattgtttaa attacctttt ataacttttg ttataaattc tagcttgact tgattattag
    56941 tccattcata ttcttccgat tctggaagaa tagatccagg tataacttct tgtgtcaaat
    57001 ccactgttaa tgctatataa ccacttgagt ttgctggaac tgttatactt tcttcttgtc
    57061 gaacaacgac catacgacct tgaataatcg ctgctcctgc agcaactttt actgttaatc
    57121 cgcttgaaga taaattcata gactgatcgt aaccatcaat aacctgattt ttccgattat
    57181 ataaaacgtg ataaagtcta gcatcatttt ctgcgcttac tttcacgttt tcaaattgat
    57241 atccatccac attgctaacc attttttatc ccccattatc ttcaaaataa tccataaaac
    57301 gacttcttat gtttccgaaa gtcaattcca caaactcctt atcacttgat attcgccaag
    57361 ctgtcaaaac cgacttgtat atctttcctt tataagaaat cgtggcaaac atccctgttt
    57421 caatcgtttc gacattcaaa ttttttgcat ttcttacaac gttcactttg atttcatgcg
    57481 aatacgtatt gcctttcaac tctgattttg ccacatcttc gtaggatgct ttatcttctg
    57541 cggtttgatc gtagatatta accaaattga cagttggttt cgtgatattt tctttcgatc
    57601 catcttgtgt caaattgttt tcttcgtcca aataccatgt tgacagtatt atcggtttct
    57661 ctatatcttt cattgcttta tcaacgatta atagcttgtt ctcgtttcca gcgcccggcg
    57721 cctgaacaaa cacatcccaa tcgctaaatt cagaagaatt gtctttaatg taaattgatt
    57781 catttacagc acgtatgcct gtatagattt ttctgttttg aattcccttg aaataccatt
    57841 ttacgttgta ttttttgaaa ccattgagaa tatacgcact taacttgtgt ttattcgtat
    57901 cggtagcttg atacgaatga gaggtcgcac tttctgcttt gacgtctaaa atatctttta
    57961 gttgtttcgt tggatcattc agcaaatagt attcaatcaa ccgccggata tgctcttcgt
    58021 agttatctcc tgacacacgt gcagtcggta tctcactatc agctaaacta agtaagcttt
    58081 tacaactgat tttttcgtct tcctgcgacg taattacacc aaaatatgca aattttccac
    58141 taggaatata ttttgctagt agaaaatcgc ctgtttttac aggaacgtac ttatccatcg
    58201 taaagctact agcctcttcg ttgatctcgt ccgcaccaaa ttcaaaactg ttagagaata
    58261 aatgttcgtt ataaagcatt aaatcacgat gaaaaagcgt gactgctaaa atcaaaacag
    58321 atcacgctcc tcgtataact caatctctac gtctgccccc ccaacatgaa acacaatgct
    58381 gaattctcca gtcggggcct gaacaaaatt agtttttgta taatcctgtt gttggtagac
    58441 agaagattct acccctgcaa tatcttttaa aatcgccgtt gtatcttcaa aaaggcttga
    58501 tacttctaga gtctgtgttt cagtcatatc tatgaagtat ccatccgttg cgatgatttg
    58561 tgagttttgg attacttccc aatacggatt tgaacacttc ccaatcacac gaatttttag
    58621 cggggacatc cgttccttgc tattcgttag gtatacagaa ttattattga atttgaatac
    58681 accttttttc tcccacaagt tttgagtgta gatataagat cttttatacg gaaaaacctt
    58741 accacgtgtt ttcactacat tcggtcgctg aattaatttt tcgcgtttta cggaatacca
    58801 gtttgatgtg aaatatagtt ctaaagtgtc tgttagtaac gatgttttcg gatcgatttc
    58861 agtcttactt aaagatttta gactgcatct tcttaccatt gtttcgccgt caaaagcaaa
    58921 ttccagttca aacggacctt ccgataggaa ctgcacaagt gaattgtaca gttctttttc
    58981 tcgaaaccca tgcacagaaa tgattacaga tgactgaaat tcagatattt caacgttctc
    59041 actgctttct ctgaaattcc cccactgtcc cacatgttct ttttttactt caaaccccat
    59101 attactcaac ccagtagcaa aataatcctc cgtagacaaa tcaatttctt cgtttattct
    59161 atttctcagt aatacagttc gcatctacat cctcctaacc aagatcttct gaatatctaa
    59221 agctaaatct ctatcagttc ggctatttcc tttgaaaaat gccagcaata acgataacag
    59281 ctgattcgtt gttgccgcct gtttttcaat ggcttctagg atttcgtaat cactggaaac
    59341 agttgtcgca tttccataag ttttaggaga aactcctagt ttgtccattg cgatagacaa
    59401 taattgcatc gctcttgatc gtttagcctt atctaaagga ataataattt ctggcttgtt
    59461 tccttctgcg atttccgcaa tttgatgttg gtttacaatt ccaccgtttg cgtaaccaac
    59521 tccacgatag gcatttgtta gtgagccata tcttgatagt gcgtatctga ttgaagctaa
    59581 gatgttagat agtgggtcaa aaatattgct gttgaatcct ggcattgcat actgtctgaa
    59641 tgttgggtca atcacttgga gcaacccttt tgatggtgtt ccatttttgg cgttaatatc
    59701 ccaattgtta actgcattag gattaccatt tgactctgta cgcatttgat ttagtaatgc
    59761 atttaagttt gcagtactgt attgaccggt cattttcaac gctctaattg ctacattgcg
    59821 ccatctttct accccactgc ctcccacgct atctcctgaa atttgagtgt tttgtgggtc
    59881 tttcacaccg tttaaatgca catgatcata gtgatctcca tcgggccatg gtctccaatc
    59941 attgtgaata cctgtacctg attgtcctga acggtcacga accttgccat ttgtgataac
    60001 atagccgatt ttgtttgcaa acttctcaaa tgcgtaattg gctgcttctg tgtagcgtgg
    60061 cgagcctcct gtgacaccag gtagtgcaat atcaatggca ttacgtttac catgcgaata
    60121 ggggtcacct ggtctatacc cactagttgc cacaaagcct ggaaacttct tcattactgc
    60181 aactgcaacg tccgccaagt atttgtaaac gccttgcatg cccattgaag tgtctaaact
    60241 gccactgctg aatagttctg tgattttgtt cgtcaatgct tcggtagcct tgcttagaat
    60301 acctttacca acatctaaag gatatttgac aagcccttcc agtacgccaa gaccatttaa
    60361 cactttccta gccaacgctc ccgggtctgt tacaaaatcc catacatcgc cgactacatc
    60421 tttcagcttg tttccaacat ctccagcaaa tcctttgacg ttgttccata gatttccgaa
    60481 aaagcctgta cctttggcgt atctatatct tggtgctttg tttccagtca tataagctgt
    60541 ttcttcagct gttagaacgt gtgtgccttt tggtgcattc aacactacat ttcgccctcg
    60601 tgggataaat gcttgtccgt taggtgtgat taccgcttca gcacctctac cgtcatttac
    60661 catcataggc ccgcccggat gacctccgtt tggtgttcct tttgcgtatt gtggcacttt
    60721 ccattcttcg agtttgtcag cacccagttt ttctagtacc catgaagctc catggatgat
    60781 tgcgttaact ggtttaccta tcgctttaag tgctgcgttg aaaatacttt tgaacgcatc
    60841 aacaatggca tttttaccgc caataatggc attcttcatc ttcgtcggta gttctgaaaa
    60901 ccaattgaat accgtatcaa tgcctctacg gaatgtgtct ttgataccgt tccacaggtt
    60961 accaattgca tcagaaactt tgttcttcaa ttcagttgct ttgttgaaaa tgttttttac
    61021 ccagccaact accttattcc aagtgtctcc aacgccatta ctgaagaagt ttttcacacc
    61081 attccatagg ttctgaaccg tattcacaac gctgttcttc atttcaatga atttattgcc
    61141 aatccatgaa gcccattctt ttattttttc ccaaagccac tgcaacacgc tccacaacat
    61201 tttgtagtat tccaccaaat tattgataac gctcataact acgtttttca cagctgtaaa
    61261 agccgcattg actatatttc taaatgtttc agattttgta taagctatca ccaaagcacc
    61321 tgctagtgcg cctaatgcta caccaatcgc cacaaaaggg gctgccaatg ttccgccagt
    61381 aattgccagc aacatacttg ctacgttcaa tgcttttact gctaatgtaa ttccgcctat
    61441 gattccgaca atccatgtta gtggctctct attttcaacg atccacgttc caatatctct
    61501 caaccaacct ataaattgag taattttagg aatagaattt tcaatccctt ttgttactct
    61561 gttgatgaaa ccagtgatat tttcaacgcc tactttgtct atgatacttt gtaacccatt
    61621 tattacagta gatttcatct gttcccaaga accactcaat gtatctgtgg aagtagctgc
    61681 tttaactgct ccgtcattca tacctaactg tacaatcgct tggttaaact catcagaagt
    61741 gatttgacct tgcgccattg catcacggaa gtttcctgta taagctccgt ttttcaacat
    61801 agcgtcttgt aacagccctg aagcgcccgg tatcgcatct gctaattgat tccagttttc
    61861 agttgttagt ttcccagctc ctgccgtctg cgttagcatc atggcaacgg atttgaatgt
    61921 atcactagaa ccgcctgcaa cggcattcaa gttacctgcc gccttggtta gttctgtata
    61981 gttaggaatc ccgttagatg ccaattgtgc ggttgtgttc agaatttctt ctaaaccata
    62041 aaccgtctta tcggcgtagt ctttcatttc ttttttcgag ctttctatct gtgacttccc
    62101 aaagttagca aactccatgg ttttggaaaa cttcatcaat gaatccgatg cgtttactgc
    62161 ttcgccaacc aagccttgca cgccacttac tacactgcta atagcgttat gcgctaatcc
    62221 agcaactgca ccaaacgaaa atgcgctttt tagcgagcct aatttgtctt ttagcccatc
    62281 cagtttccta gctgaccttg tggacttgtc gccaaaatct tctatttttt ttcctgattg
    62341 atcgctggag cttttgagtg cttctaattg cctgctagat atttggcttt gtcgttctaa
    62401 cttttctaat gccctttttg catcttcggt ttcatttgct gaatcgccaa actcatcagc
    62461 catcagtttc acaactttgc gctgttcttc gatagctttc tcggataatt ccgtttgttt
    62521 ggctagccct ttttgttttg cttcaaacgc accagattca tcaccagcgg ctttcaacgc
    62581 ttttacttcg gcgttcattt gtcgttcatt ttctttgatt tcgttagata aatcattgac
    62641 ggctgttttg gaatacacca attctttttt tgtgtcattc aactggcgac tgtaagcatt
    62701 atattttgcg gtagcattgt ttatctgtgt gttaaggtta gcaacttgtt tcgattcctc
    62761 gccatacttg ctaatcgctt catcacggcg ctttgttaat tctcttactt tggcgttttg
    62821 cccttccata accgtagaca agtctttcgt cttttgacta agtgcttcgt atgaacgtcc
    62881 tgctgaatca taagccttta gattggcacg catattcgac tcagcttgtt tgactttcgc
    62941 attgatttcg tccagcgtgt taccaaaatt agtgctatct aaactaatcc ctagcttgat
    63001 atttcctgcc ggttgtcctt ttcctgccat tatttacctc cttcctcaag ttttaccaag
    63061 tcttcagccg ataaaaattg tttgatgaaa tcagcaccat ctacatattc ttcgccactc
    63121 tccacttctc caaaaaggtg taacaaataa tgatagtcgg cttcgtccac atctctcatc
    63181 gtccaacctg attcgattaa atctttgtag atttgatcca ttgctttcct agcttcagaa
    63241 aaacttatct ctttttgctc gccgtctgct ttttttcatt gtttcccagt tcattgattt
    63301 gttcaaaaac actttctaat gccggtacta actcgctcgc agtcaaaccg tctaaaatag
    63361 catcaaatgt aactgctgga tcttggaaaa tatctgctgt aattgcaatc attgaatcaa
    63421 ttgcttctaa atcagttagg tctgcttttt ccgctttctc gtaaaatttg atacactcac
    63481 gcattgcacg tgcggaaata tcttgttgtt tgaatgtttt tttctttccg tcaagtttca
    63541 attgcaattc aatcatttgt tttcctcctt gtttttacaa aaaaataagg ctagccaaaa
    63601 aatggctaac cttgtgtatc aatttttggt tctggttctt ttggtgtccc tgtatctgtc
    63661 gttggtgtag atgcagggtt aactactccc ccgctttgtt atttaccaag tccttgaatt
    63721 tttgtaaggt catttcttct gattctacgg ctgttaagta tacatagcca cgttcatcag
    63781 aaatgaattc cccttcgata gaatcggttt gcaattcaac tcctttgtct tcagctgttt
    63841 tcatgtcgat atctggatga ctgaattttc cttttgccaa tcccatgaat aagcgttttc
    63901 cttctttgtt cgctgtaacc atgactaccg acacgtaagg cgcttcagtt tctgaaccaa
    63961 ttacatttac accatccacg gttttagcgc caatgatttc gctgtaaatg ccgttatcca
    64021 ttaagtctgc cacgtcaagc gtaacttttg gcgacgaaac ccctttactt gcaatgaaga
    64081 atggtacgtt tgaagcgtat gttgtgttag aagttgcgcc taatccagta attttagctt
    64141 cgatcgctcc gcctttcgac ttatctgcta ctaattcttt tagagtgccg cctgcacctg
    64201 tttttacgcc aaaaatgacg ctctcaaatc ctactgttgc catctatttt ctctcctttt
    64261 aatttagtga aatatttgct acatatcgtt tgataatccg ctttgcacct tctaaatcct
    64321 catcatctgt ttgttccgtg tatgcgcatt gccaaccatt ccccctcata atctcatcaa
    64381 gggcaaaata aaaggcatca acctctttta aggtcgatac ccatacatct acctgtacgt
    64441 taaattgaat ggtcaaagga ttgttgcttg cgaaatcttc atagttgccg aatatctctg
    64501 taattctgcc aactggaagg ctaggtactg tttgagccga ttccggaaca ctattggtgt
    64561 aaaaatcaat gttctttgtt ttttcattgc tattcagaat tgaatagact tgtgatactg
    64621 ccgttttcaa agtcctagcc tcctttttac ttcgtcagca atgatttgtg ttacttgttt
    64681 ttcgatttgc ttttgtgttt tttgtacgaa accttttgga cgttgtttga ttgttccgaa
    64741 ctcgataaag tgcatccgcc aagaaacatc tttatcatag ccgacttcta tcaatccgtt
    64801 ttttaccgag cttgtaacca catggtcctt agcatgttct tgcatatacg aaccacgttt
    64861 accgtttgac ttcgttccat cccagtaagg tgtgttttgt tgtaactttt cttgagcgta
    64921 ctccccagct tttctaagtg ctgggctttc cactcgttga acgtttgctt ttacttccct
    64981 aagcgctttg tacacttcgg ttgcatcgac ttctacactc attttgaaac ctcttttgca
    65041 atgattgtcg tgaagtcctt cgcaaactcg cctttcgtaa tcgtaatgat ttcaaacgtt
    65101 tttccattcc aacgcacttt catatcattg gttagctctg atttttgttg atagcggata
    65161 atgaacgtca gtgttccttc caaagccgta ccaatggacg tcttaatatc gttcaggcgt
    65221 tgtgtctgca cacttgccca gcaagtaaga atggttgtag aagtccggac aacttgcccg
    65281 tcctcgtcct tgaccgtcgt atctcggaca aacttgatgc gttgatttaa atttcctgtt
    65341 tgaataaggg gcatacgcta ctcctccaca aaaagcaaat atcttgcttt gagttgcaag
    65401 attaggcttg taaaacctaa atcgtactct cgcaagttcc cactcacggt tgctgatcga
    65461 gcgttgtaat aatgatctgc taattgcaga atagctaaat taatcagatc aaccgtgtca
    65521 ttttcttgct tataaaaaga gggcttatca ttcccgatag cccctctaat gtatgcaatt
    65581 gcagcttgca ccgcacgtct tacttccaca tcgtcatcat cggtatcaat ctttagtgcg
    65641 tttttgattt cgtctaaatc cattttagga tcaagaatca taagaaatca gctccttaac
    65701 cgtttgttac tggcacttcg tttgcgatag atgtaaatgt agctaaaaca actgcttcat
    65761 catcgactaa ctgcacatca aatcgatcga taacccgtac tttggtggtg tctgtttcaa
    65821 aagccccccc gccaatattc gttgtcagca agctcatgtt ttcgcgatca tacaatgtaa
    65881 cagcttcttt cagatcacca atatacagtg gatatttagg agtagcttgc gtacctttgt
    65941 ttggcaagaa acgagaagca attttcttga ttggtttacc taagaaggta tatccagttg
    66001 cagaagctac gtctttttgt aacaagtaag atccatcagc acgtttcact ttatctaaaa
    66061 cattgaagcc atcttggttt gtaataaaca tagatgtagc ttcgatagct ggatcaagtt
    66121 ggacgtttat gatatcttta atcccatcaa catctgtaac atctttcttt tgcgctgctt
    66181 tgattccatc aatagctgcc aagatttttg tattgcgagt aacaactact tttttcgcga
    66241 tccagttaga caaccatgcc aaaatgtttt cggcagtatc ttttagcaag ctgttagtta
    66301 ctgtagaaat acctgcgtag cgtttgatca agtatttgat caagtaaagt gcaggatcat
    66361 catttgctgg gatttcaccg tcttcagtat ccaaagcggt cagtgggtta atatcagacc
    66421 atttttcata aacccgagaa ccgctagtag tggtcacttt ttcaacgttt acgtattctt
    66481 gcaaagagtc aaactgacga accaaagtat gaatagtcgt ttgcacatct acaggaatag
    66541 tcaatccgat agcattacca gattcatccg tattagatgt caaagtagcc ataatagcag
    66601 gatcaccatt gaccatcgct ttaaagtctt tgataaactt gtttttcaag ttttcatctt
    66661 ttttatctaa tggttctttt ttgacgttta agacttgttc ggcttccata tttgccactt
    66721 gctctttcaa tccgtcccgt ttagcccgtg ctgcttttac ttgtgcttgc aaacttacta
    66781 catcttcttc tgttttttca tcgtcaacca atgctgcatt gagttgcgcg tttaaatcag
    66841 agactttact tcccgcctcg acccacgcat tttttaattg ttctaaattc attcgttttt
    66901 ccctccattt aaggctttta gttttttctg caagagcgtt tcttcttgcg gtgtttccgc
    66961 tttgagtatt agatttttca acttagttac cgcgtttttt ggaataaccg gttgagaggc
    67021 attgagtacc gttactggtg cttctgcaaa cataatttca tccgcaaaac cttccgccac
    67081 tgctgtttgt gcgtttaacc aagtatcttt tgccattaaa tcagcaagct ttttccggtc
    67141 gagtcctgtc ttgatctcgt aagcattaac gatagactca tccacactgc ttaacatttc
    67201 cgcattggct tttaattcct cggcgttgcc gctagtggtc acccatgcgt tgtgaatcat
    67261 gatgtgtgct gttggcgaga ttctaagcgg ttcgcaagca caagcgatta cacttgcagc
    67321 gcttgctgca atactcacaa catttccgga tacctttccc ggataggcac ggatagcggt
    67381 atatatttcg cttgccgcta ggacatcccc accgttagat gagatatcaa gttctacttc
    67441 atcccccgct gcttctgtaa gagcggcaga aattttccct ggtgagatac aatcaatccc
    67501 aaaccagtca tataaccacg cggtatcgtt atccacgaca tcgccgctta acgtcacttt
    67561 tttcatcctt tcacctccct tcggtgcaaa ctaaaaaagc ctaaccgttt tcggctacgc
    67621 ttttagacga gtttgttttt cttaatgttg gatccatatc gattggatat aggtctccgg
    67681 aaatatgata atcatccata ccggttttat ttatcggttg caagtcctca aatcttcgga
    67741 catcattcgc tgagtatgct ccgcctcgtc gcatgatttg atagaattgc ccccttgcct
    67801 gagtgtcagc tcttagcaaa cttgcgacat taaacttata tcgataacct tttgcttttt
    67861 cggttctcga tagtgttttc ttatttagtt ccgcttcata ttgattaacg gtcggtacta
    67921 gattgtatgt caaaaactcc atattgagct gttcctgtga agagtaactc gactggttac
    67981 tcccaatgaa atgttccggc acgttgtaga ccatcgcgat tctggagcga gatactttat
    68041 cagtatctaa aagcttgctg tctaccagct ctcgttgtaa acgttcgatt tctacaccgt
    68101 tttcttccac aagtagtcca ccgttttgtc gataaaaatc agcaatgttt ttaactgtag
    68161 cttttttagc ttcttcatcc acattgctag caaacttaac ttttagccct tcattacctc
    68221 ctttaagctg acttaaagag atttttcgga cttctcgatc atatccgagg gtattcgtta
    68281 atacttttgt ggggtctgta ccttccaagc caccaaacct cggttgttta aagtggagca
    68341 tttccatgta atgtacataa ataacttgct tataactgcc tttgtcagtc gcagttactt
    68401 ggtagtaaag ctccccgcta tccatatcaa tcactggatg gcaagcacca ggttttatta
    68461 acgccatatc tgctacttct ccattgatat ttcggaaaat ctgcacatac gcattccctt
    68521 ggtagtttct caaaacttcc acgtcccgga aaaaatcaaa ctttgtaaaa tatcgagggc
    68581 cttcgctcaa caagttgtat gcaggacagt cgtccggttg gccaaactcc gcatctatca
    68641 ttttgagagg gagagacgca aaaacgttcg atacacggct aattacggaa aaaatccctt
    68701 ctgatatttc atcttttccg actaaatacg gtatcaatcc cgggtcattc aaaaaatact
    68761 cttgtttgct cgctttcggc ttcgctcggc caaaagaacg caaacgatct aaaatactca
    68821 ttcctccaca cctttcttac atattcatta aatcggaaat cgagtaatag gttactttcc
    68881 cggttccgac aggattaacc aacatattca acacttcggc gtggctgttc aaacttgctg
    68941 caaaaccatc tatttttcta ctcttggact gcttagacgg catccagttt gagttgcgat
    69001 ccatcaccag cttgacgttg gatagatacc atcggtaaag cttggaattg ttgaaaatca
    69061 ccttaccgtc caatagcatt tccttgaagt tttgcattgg cccacctaat gatagaaacc
    69121 cttgccgaat ctcgttggtt tcaaatcctg cattttctag ttctttattt aatcgtagcg
    69181 ccttggcctt gtcataattg atttttacaa tgtcatagat tttcgaattt tctacaaacc
    69241 aatttaagac gtattcataa ttgacgtaat caccaggaat aatcgttaga tctcccacct
    69301 tctcccacgc tttgatacgc tcttgattgt tatctcgatc aaatctagct tgtgggatcc
    69361 atgtgtgttg caaaatgaat acctcgcctg tttcaagcgg aaattctaaa acggctgcgg
    69421 taaagtcttc cgtttcggac aagtcaaaac caccgacaca cttcttacct ttgagcgttt
    69481 caatatcgat ggttttattg tttcgtttaa tggtaggcat atcgacaaat gacagctcat
    69541 cgatatcgga aaacaagtta aattgcttgg taatccagtc agcatattct tttggatctt
    69601 ttttatcctt aacatagtca tctagcatgc caacaaaatt cattagacaa atattcggat
    69661 tggctttgat ccataatctt gggtcatcag cctcttccgc actgtcaagt tttgccagat
    69721 aatagaaagt ccgttcatcg atgtcatctt ccaaatgttc caaacagtcc acaccttgct
    69781 caaaataaga catcaacgga ccatctaata catatccagc agttgtgata taaactatca
    69841 gaggctgttt tctggttcct cttgatttct tgataacgtt gatcagattg taattcgtaa
    69901 actcgtggat ctcgtcaaaa ataccaaagt gagtgtttag cccatccaat ttccggctat
    69961 ctgaggcgcg tggttccatt ttagaaaagg cgggagcata attaatactt gatcgtttag
    70021 gcttaccaaa tttcttaaag agcgccggcg attgtttgac catttctgcg gccttatcaa
    70081 acaacaagct tgcttgatca cgagcatttg ccaatacgta aacgttggcg ccttgttcat
    70141 catcataagc gaccatgtat gtggaaaggc ctgaaattag tgatgttttc gattgttatt
    70201 atctgcgagc tttttatctc gcactctgga ggtcaccctc attttcatcg gtcagtcagt
    70261 tctggcccag tttagcgtac attttcagca aattatctgc tgtcggacac tcttgggagc
    70321 attatattta ttcaactcct acgcgttacg gtagttagta acctttcgca atctactaac
    70381 ttacctcggt atcttcttta tgtttgaaca taattccttc gatagctaat attttttctt
    70441 tatttctgta gttcgcttta ggatttatca cataaggggc gccatctttt aaaagtcggt
    70501 aaaaaacaca tgtactaatc gaatagtaag ccatgcaaag cttagcgctg tgaaattctc
    70561 ttttagttcc atcccgttca attgcgacaa ttggttttga ggttacagca ttttcaccac
    70621 tatatgcttt ctttttcata attgattctt tgctgtggtg ctttcccaac atgccttttg
    70681 ggtgttcgtg agtaacaccc catacaacat caccgtttgt catacaatta ttcttatggt
    70741 tctttgccca tttactgtga tttgaagaaa attctttaga ttgttttttc cctagcattc
    70801 ctttggggtg aactttatat attattccgc catttccgcc ttttgcgatg ttataacctt
    70861 ttttcttatt agttgagtca taaagttcaa tataaaactt ttctttctca aaagcttctt
    70921 tcatcgttaa gtcttcttca ataatcaaat gatcaaaatt ttcccacccg tatttttgta
    70981 ttgcattcca aaaacttctg tggtgttggg tttcgttctt tggtggttta tattcaatcc
    71041 caccgctcct ccaccttctg ccaatatcat ttgtgacacc gatataaatt ttgccattta
    71101 ttttatttaa gtgtttgtaa ataatgtatt ttttcaatct actcacctct tttatatact
    71161 accatcaatt gatggtagtt gcaagcggtg gaatcggatg ttatagtaaa ataaaaagga
    71221 gtgtcacaca tggacaaata cggactaaaa aacagaatta gaatttctaa cgcaattgat
    71281 aaagatttat acgaaggatt aaaaaaaatg tcggaagaaa ctatggttcc tatgtctaaa
    71341 ttgcttgata aagctatcga attattacta aaagaaagcc aaaaataaag gttttcaccg
    71401 attttgcccg atttttaaac ggcaaaactt accgttttta cgtccaacaa aaataagagc
    71461 ctcgcggaag cgacgctctc ctgtatcacg atgtacccat ccgtacatgg atccaattat
    71521 gaaatgctgc caaggttgca aaacaaacga accaaagtcg ccttcagttg gtttacattt
    71581 tttttcgata tatctaatag gccggtgacc tttttcttcg tcaaagatcc aaggaaaatc
    71641 atctgttcct tggcgctgca aatcacgcat atgccgttta gcagcttgaa tattttcttt
    71701 gctggctggt atgcttccat cgattaatcg ttccgcatac caagtagtta acaattccgg
    71761 atatggacat tttaaaaagc caccccaaga agcttgctcc tcaagatagc tttgccaata
    71821 gtccacacgt tctgtgtagg acatatccaa aatattagaa gtcgtcatcg tcatcaccac
    71881 catcatcagc catcttaata gctaacttag ctctggctgc tggtgatagt cctaaatcag
    71941 cgccaaaaga acggagattc cgcgacgctg tatccatctg ccgagagagc ggattaccaa
    72001 ttaattcatt aggttcatta aacggctcac ctctcgcctc tgcctccttt ttcgcaagcg
    72061 catagttcaa cttgtactct cgttgtagtt ttcgaagctg tttttctaaa gaaacgtatt
    72121 gcgaatacca attggagtag agagccattg tatggacgtc cggattactg attaaatcca
    72181 cagacagcaa ttcatcagcg ataaactcaa aggtatcctt tcctagcgaa tctagccata
    72241 acggcggttt gattttgtca gtagccattt ttaatttgtc ttctgcggcc gctcgcttgc
    72301 ggagttcttc ggtattcttc ttatttgggt ttccgtttaa taattgtaat tttgcactct
    72361 tcgctggttg cggcataata tcaccttctt tcaaaagata tattgaaatc gttatcacaa
    72421 tccttataat taagttatcg gcagtgctgt gccgaaattt ttttagggag ggattcctat
    72481 gggagaactc tataaacccg gcgaagacaa taaaccaaaa ggaacctata aagaagtggg
    72541 acctcgtggt ggaaatgtcc aaggcggacg agaagtaaaa attgatccag gcgaccgatt
    72601 acctcctact tctcaaaaag gaaacaaatg gactaagaaa tagcttcgtc caaagccggc
    72661 ttaaattggt cggctttttc ctttttctcc aaaaaacttg aaaagcggtc tttttacgaa
    72721 ggaaggacag caccgttctt ctcaagcctt actctttaat cttttagagt aggggggcta
    72781 ccttcatttc gctgcaaact tatctttcag gagttgaata cattttaacg atatgtgatt
    72841 taggttttgg tttcttcttc ccgcctgatc tctctggatg ctctctgttg tgacaagcta
    72901 cacagataca ttcaaggtta tctacggacc agaacagtga caggtcttcc ctcgcctcga
    72961 ctatgtgatg gatgatcgtg ccccttgtgt tccgccctcg acgcttgcac tcttggcaca
    73021 tgccgaagtc cctagctatt actacttgcc ttaggtctcg ccatcgtttg gtcttgtata
    73081 gtttgtctat ctcgtctcta ggtctagctt ctttcattta cacatctctc ttgtgtccga
    73141 ttgtttttat atctggatac ttagttactt cacctaggta ctccatattg atgtcgtttg
    73201 ttgaaccagc taccacatca gttgttgtat ggtagtgata gcttacatct atcaatccct
    73261 ctaacttctg acctttgtaa taaacctctg gtactgagtc agtatctttt agtttgatct
    73321 ctagaaggtt aggttcttgt ttgttattca gatactttag gctttgttca agcgatttca
    73381 ctgatttaac tcttgtttcg tctgtcaaag gaataatatc ctcgtgttca tcactatcca
    73441 aataatcatc ctgaaagtat tctaagctaa ctttataatg tttggctatt gcacatatct
    73501 tattaaagtc agcgcgttgc ttttttccag actcaatatc agatacccat gattttgaca
    73561 gtccggtttg tttaactaag tcgttcattg tttcgttatt aagccgtctc aatttatgta
    73621 tcttatctcc tatatgcatt actcttcctc cttaatc
//
LOCUS       DAYYCW010000003        60380 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000003 DAYYCW010000000
VERSION     DAYYCW010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.003"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1421)
                     /locus_tag="ACHAYA_000174"
     CDS             complement(<1..1421)
                     /locus_tag="ACHAYA_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFM8604011.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
     gene            1673..2854
                     /locus_tag="ACHAYA_000175"
     CDS             1673..2854
                     /locus_tag="ACHAYA_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8604012.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(2914..4542)
                     /gene="treC"
                     /locus_tag="ACHAYA_000176"
     CDS             complement(2914..4542)
                     /gene="treC"
                     /locus_tag="ACHAYA_000176"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM8604013.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(4993..7704)
                     /locus_tag="ACHAYA_000177"
     CDS             complement(4993..7704)
                     /locus_tag="ACHAYA_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8604014.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7714..8415)
                     /locus_tag="ACHAYA_000178"
     CDS             complement(7714..8415)
                     /locus_tag="ACHAYA_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8604015.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8601..9200
                     /locus_tag="ACHAYA_000179"
     CDS             8601..9200
                     /locus_tag="ACHAYA_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8604016.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9281..10294
                     /locus_tag="ACHAYA_000180"
     CDS             9281..10294
                     /locus_tag="ACHAYA_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM8604017.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10402..11319
                     /gene="rbsK"
                     /locus_tag="ACHAYA_000181"
     CDS             10402..11319
                     /gene="rbsK"
                     /locus_tag="ACHAYA_000181"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM8604018.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11347..11742
                     /gene="rbsD"
                     /locus_tag="ACHAYA_000182"
     CDS             11347..11742
                     /gene="rbsD"
                     /locus_tag="ACHAYA_000182"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM8604019.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11742..13115
                     /locus_tag="ACHAYA_000183"
     CDS             11742..13115
                     /locus_tag="ACHAYA_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8604020.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13472..15616
                     /locus_tag="ACHAYA_000184"
     CDS             13472..15616
                     /locus_tag="ACHAYA_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8604021.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(15668..16708)
                     /locus_tag="ACHAYA_000185"
     CDS             complement(15668..16708)
                     /locus_tag="ACHAYA_000185"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8604022.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(16985..18787)
                     /locus_tag="ACHAYA_000186"
     CDS             complement(16985..18787)
                     /locus_tag="ACHAYA_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8604023.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18801..19553)
                     /locus_tag="ACHAYA_000187"
     CDS             complement(18801..19553)
                     /locus_tag="ACHAYA_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8604024.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19562..19924)
                     /locus_tag="ACHAYA_000188"
     CDS             complement(19562..19924)
                     /locus_tag="ACHAYA_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8604025.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20100..20972)
                     /locus_tag="ACHAYA_000189"
     CDS             complement(20100..20972)
                     /locus_tag="ACHAYA_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8604026.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21023..21511)
                     /locus_tag="ACHAYA_000190"
     CDS             complement(21023..21511)
                     /locus_tag="ACHAYA_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8604027.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(21649..22440)
                     /locus_tag="ACHAYA_000191"
     CDS             complement(21649..22440)
                     /locus_tag="ACHAYA_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM8604028.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22495..23103)
                     /locus_tag="ACHAYA_000192"
     CDS             complement(22495..23103)
                     /locus_tag="ACHAYA_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM8604029.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23096..23413)
                     /locus_tag="ACHAYA_000193"
     CDS             complement(23096..23413)
                     /locus_tag="ACHAYA_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8604030.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23569..24297)
                     /locus_tag="ACHAYA_000194"
     CDS             complement(23569..24297)
                     /locus_tag="ACHAYA_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM8604031.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24456..25337
                     /locus_tag="ACHAYA_000195"
     CDS             24456..25337
                     /locus_tag="ACHAYA_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8604032.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25574..26617)
                     /locus_tag="ACHAYA_000196"
     CDS             complement(25574..26617)
                     /locus_tag="ACHAYA_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8604033.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26620..27288)
                     /locus_tag="ACHAYA_000197"
     CDS             complement(26620..27288)
                     /locus_tag="ACHAYA_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8604034.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27430..29766
                     /locus_tag="ACHAYA_000198"
     CDS             27430..29766
                     /locus_tag="ACHAYA_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM8604035.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29881..30192)
                     /locus_tag="ACHAYA_000199"
     CDS             complement(29881..30192)
                     /locus_tag="ACHAYA_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604036.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30373..31848
                     /locus_tag="ACHAYA_000200"
     CDS             30373..31848
                     /locus_tag="ACHAYA_000200"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM8604037.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31895..32479
                     /locus_tag="ACHAYA_000201"
     CDS             31895..32479
                     /locus_tag="ACHAYA_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM8604038.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            32481..32864
                     /locus_tag="ACHAYA_000202"
     CDS             32481..32864
                     /locus_tag="ACHAYA_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM8604039.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(33181..33465)
                     /locus_tag="ACHAYA_000203"
     CDS             complement(33181..33465)
                     /locus_tag="ACHAYA_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM8604040.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33817..34527)
                     /locus_tag="ACHAYA_000204"
     CDS             complement(33817..34527)
                     /locus_tag="ACHAYA_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM8604041.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34524..35996)
                     /locus_tag="ACHAYA_000205"
     CDS             complement(34524..35996)
                     /locus_tag="ACHAYA_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8604042.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36134..36607
                     /locus_tag="ACHAYA_000206"
     CDS             36134..36607
                     /locus_tag="ACHAYA_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8604043.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36760..37311)
                     /locus_tag="ACHAYA_000207"
     CDS             complement(36760..37311)
                     /locus_tag="ACHAYA_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8604044.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37416..37946
                     /locus_tag="ACHAYA_000208"
     CDS             37416..37946
                     /locus_tag="ACHAYA_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM8604045.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38429..38872)
                     /locus_tag="ACHAYA_000209"
     CDS             complement(38429..38872)
                     /locus_tag="ACHAYA_000209"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8604046.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38888..40048)
                     /locus_tag="ACHAYA_000210"
     CDS             complement(38888..40048)
                     /locus_tag="ACHAYA_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8604047.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40555..42255
                     /locus_tag="ACHAYA_000211"
     CDS             40555..42255
                     /locus_tag="ACHAYA_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604048.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42357..43190)
                     /locus_tag="ACHAYA_000212"
     CDS             complement(42357..43190)
                     /locus_tag="ACHAYA_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM8604049.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43494..45659)
                     /locus_tag="ACHAYA_000213"
     CDS             complement(43494..45659)
                     /locus_tag="ACHAYA_000213"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM8604050.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45919..48213
                     /locus_tag="ACHAYA_000214"
     CDS             45919..48213
                     /locus_tag="ACHAYA_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM8604051.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48206..48871
                     /gene="pgmB"
                     /locus_tag="ACHAYA_000215"
     CDS             48206..48871
                     /gene="pgmB"
                     /locus_tag="ACHAYA_000215"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8604052.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            48864..49871
                     /locus_tag="ACHAYA_000216"
     CDS             48864..49871
                     /locus_tag="ACHAYA_000216"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8604053.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49893..50912
                     /locus_tag="ACHAYA_000217"
     CDS             49893..50912
                     /locus_tag="ACHAYA_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8604054.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51193..52560)
                     /gene="nhaC"
                     /locus_tag="ACHAYA_000218"
     CDS             complement(51193..52560)
                     /gene="nhaC"
                     /locus_tag="ACHAYA_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8604055.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52669..54090)
                     /gene="tyrP"
                     /locus_tag="ACHAYA_000219"
     CDS             complement(52669..54090)
                     /gene="tyrP"
                     /locus_tag="ACHAYA_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM8604056.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54333..56210)
                     /gene="tdc"
                     /locus_tag="ACHAYA_000220"
     CDS             complement(54333..56210)
                     /gene="tdc"
                     /locus_tag="ACHAYA_000220"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8604057.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56501..57757)
                     /gene="tyrS"
                     /locus_tag="ACHAYA_000221"
     CDS             complement(56501..57757)
                     /gene="tyrS"
                     /locus_tag="ACHAYA_000221"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM8604058.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57869..58074)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58337..59638
                     /locus_tag="ACHAYA_000222"
     CDS             58337..59638
                     /locus_tag="ACHAYA_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8604059.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59707..60306)
                     /locus_tag="ACHAYA_000223"
     CDS             complement(59707..60306)
                     /locus_tag="ACHAYA_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8604060.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        18228 a        12352 c        10821 g        18979 t
ORIGIN      
        1 tttcctgcat ataaatgaaa atgttcggag tcagccggtg caatcgtgtg atcactgaat
       61 tgaatatatc taaaagcgcc actttcctta tcttctgctt cgaatagata ccttacgcca
      121 cggtttcctg cctcgtaggt caagatttct ttccctacat aacgataatt tgaacgtttc
      181 gttgttccat tcttaaacgt aaatgccatc tctttctcac tgatcttgat tgaagcaata
      241 tctgtttgat agcccttcgt ataatattct ttatattctt gtgctgtctt atcctgattc
      301 aatttttctt tatattcaaa aacaggatca aaagtcccat cttctacata cgtatctact
      361 gtttgccaag ttcctgccca atctgaaaga gagcgatctt gtacatcctc gtcttcaaaa
      421 taaccgttgt agacgctctt ttcttttttg ggggtcaaag tatcttctag cgggttttct
      481 gtctctgtcg tttttttcaa cgcttcgagg tttgcttcca tgatagaaag atagttttct
      541 ccattttcta tttgttcatt cgtcaatcct tccaaaggat tcaatacttc taaagataca
      601 cctgcttcgt ttgctaacgt tctggcaata ctatctttgg cgttttcttc aaaataaata
      661 tagttgattc catgttcttt aacaaagtgt ttcagttcag caattctcgc cgcagacggt
      721 tcttcactag aagaaagacc agcaatcgat acttgattca atccatattc taacgctaga
      781 tagttgaatg cagcatgttg cgtgacaaaa gatctttgtt ttgcttgctt aaaggtttct
      841 tgataaagct gatggagtgc ttgaagtttc tcggtatagg ctgctgcatt ttttgtccat
      901 acttcctgtt tttctgggta agcttcaatc agctgatcac gtatgtttgc tacctgcttg
      961 attgctaaac ttggagctag ccatacatgt gggtctagct catgtgtatg gccttcttcc
     1021 ccgtgttcat ggtcatgatc atgtccttct tcacttcccg gaagtaaaag cataccttct
     1081 gtcgctttta tcgtatggac attgccttct tgtaatgttt tttggattgc ggggacccat
     1141 gtttccatgt tttcatcatt ataaacaaag atatctgtat cttggatctt tgccatgtct
     1201 ttagcagaag gttcataatc gtgggaatca gtccctgctg gaatcaataa ctctacttct
     1261 ccttcatccc cgacgattgc ttttgtaaaa tcatacatgg gataaaaggt cgtcatcacg
     1321 ctcaattttt ctttactgtc ctcggctttc ccacttgccc cgcaagcagt taacatacct
     1381 gctaaaacca gcatggccaa tatacctatc aatcgtttca tttatgacac tcctcttctc
     1441 atcttctttc atttgaatgt tccgattcgc ttttcgcaaa ccgtaatagt tacgttttag
     1501 caacaggatt taatttatca tatggttaaa agaactgtca agcgttttct tattttaatt
     1561 gatgagaaag tttatatgaa tgattgacga tatttttgca atcaacctct atcattttcc
     1621 ttttataccc gcttttgtct ataatgaagt tatagataaa ggaggaaaaa gagtgaaaaa
     1681 agaaacagat acaacgaatc gtttgatccg ctttttagga ggaaaaacct cttactatgt
     1741 tttaggaata gttatcttac ttgcgctgat catttatttt ttccatcaga tctcgtttat
     1801 attcaagcct gtcggaatca ttatcgctac tatcttgccc ccattattgt ttgcactgat
     1861 cttgtattat cttttggaac cagtcatcaa acgtttgagc aagcaccttt cgaggaactt
     1921 gtcagtcatt atcgtttatg tgattatttt atttttattg gggcttggaa gcatatggct
     1981 tattcctttt cttgaagaac aggcaaagac attgatcagt agtttgccag atttgtttca
     2041 agattttcaa gagagtctcc gacaattttt ggagaaaaca ccttttgccg attcatttaa
     2101 tcaatttttc gcttctattg acgacgtgac gtctgatata gctggattta ttggaaatta
     2161 ttgggaaagt ggcgcacaac ggatcagcaa cattttttca actgttacag ctatttttat
     2221 tactttattt actggtccaa ttgtcgcttt tttccttttg agagatcctc aaaaatttta
     2281 tcagtcagtt ttagcgattg tcccaccagc atttcgtaca gattttaaaa atttgacaaa
     2341 gattgctaat caacaattgg gttcatttct aaaggggcaa atcattgctt catttatcct
     2401 tggagcagtc tactgggttt gtttcttact gatcggatta gaatttgcta gtgttttagc
     2461 tatttctgct gggttgttat gtatcattcc atatattggg ccgtttatcg ccttctttcc
     2521 aggtttgatc attgcattcc aagactctac ttttatggct gtcaaatttg tcattgtctg
     2581 gtttgccgta caacttctcc acggtgactt ggtgattcct cgagtaatgg gtgatcgttt
     2641 acagatccat ccgattacga tcttaatcgt tttacttgtc atgggtgatc taatgggaat
     2701 tgtgggcgtt atttttggta ttcctatcta tacgttagtc aaattactcg ttacttttat
     2761 tttccgtaaa ttcaaacaac gctacaataa attttatggc gataaaggag agtatgaaaa
     2821 ctcttcattt tctgaagaag attattttaa ataaaacaca aaacgtcgat cttacgagaa
     2881 tagctcgtag gaacgacgtt ttgtgttttt gttttaattt ttcagcaaag caattgcctg
     2941 gtatggtttt agctccagac tttgatgaag aacagtatcg gaataattat cgataagaat
     3001 attggcttct gtgaatcctt ctgggatggc gatccttgta tctttatcat agaaatttgt
     3061 taatacaagc agctgctgtc cttcaaattg gcgaagaaag ccataaactt ccggatgatt
     3121 tgcttcataa gcatgataat ctccttgtgc aacgattgga tatgtttttc tcagccgaat
     3181 caatttcttg taataggaca aaatagaatc ttcagaagat agttcttttt ctacattaat
     3241 ctcttctagc ttgcctccaa cttttaacca aggtttccct gtagtaaatc ctgcttgttc
     3301 tgtgtctgac cattgcatgg gtgttcgaga gttatcgcga gattttgctt tgatccgacg
     3361 aaacgcttct ttttctgtga atccttgttc aaggagtaac ttataagcat tcaaactttc
     3421 tacgtcaata tagtcggaaa tcgatgaata gtctgggtcg atcatgccga tttcttctcc
     3481 catataaata tagggcgtcc cacggttcaa atggatcatg gttgctagca tctctgctcc
     3541 ttgttttcga tactgatcga tagctacaaa tcgattgagc gcacgtggct gatcatgatt
     3601 attaaaaaat agagcattcc agccgttacc ctcactcatc ttttctcccc atgtatgaaa
     3661 tagtcgcttt aattctgcaa aatcaaatgg aggagtagac catttattcc cattttcata
     3721 atcgactttc aaatgatgga aattaaagac cattgataat tcttctctac taggttctgt
     3781 atacaagata caattttcga tcgtcgtaga gctcatttca ccgactgtga tgatagtgga
     3841 atccgtccca aacgttgctt ggttcaattc ttttagatac gtatgggtga ttggtttgtc
     3901 cgtatattca ggtttgccat cattttcttt attatttttc aattgttcgt ctttaccgat
     3961 aacattgata acatcgaaac ggaatccttt tacccctttt tccatccaaa aacgaacaac
     4021 tttgaatagt tcctctctta ctgcttggtt ccgccagttc aagtcagctt gtgtccgatc
     4081 atataagtgc aaataatatt tccccgtatc tccaaatggc gaccaagcac ttccaccaaa
     4141 ttttgatacc caatccgttg gctcttctct taaaatgaag aagtcctgat aatgctggtc
     4201 ccctgctagt gcttttttga accattcatg ttctgtcgaa acgtgattca gcaccatatc
     4261 gagcatgatt tctatccggt atttctttgc ttcggctatt agctgttcga attcctccat
     4321 cgtaccaaag ataggatcga tggctgtgta atcagagata tcataaccgt tgtctttttg
     4381 agggcttgga taaatagggt ttagccaaat catgtcgatg cctagatcat tcagatacgg
     4441 gagtttttct ttgattcctt ttaaatctcc aattccatca ccattactgt ctttgtaaga
     4501 tttgggatag atttggtaaa tgactttgtc tttaaatgtc atggatattt acctccattc
     4561 aactgattta ttacctacca caacttatat atacaagtta tcatttatta ttataactga
     4621 accgaaagcg tttgtaaaat ttatctttat acattctaag aggtctgttg cccaataaaa
     4681 aacaagagat cagacatgta tttgtctatc ctcttgttta aaatagcaaa atattcttgt
     4741 ttcttctata aatggtgttc aaaagctttt atctacggca atcagtcttc tcaaatgtaa
     4801 aacatgagga gctaatttta tttgagtatt ctttttgctc gatgcaggac ggctttttta
     4861 caacctctga ttcatggtgt tcaatcagct gtccttcggt attagctcaa aaaacggcaa
     4921 aatatgaaaa actattttgc cgtttgtttc tctaatactc aggtcaaacc gctgatttca
     4981 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5041 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5101 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5161 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5221 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5281 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5341 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5401 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5461 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5521 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5581 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5641 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5701 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5761 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5821 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5881 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     5941 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6001 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6061 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6121 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6181 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6241 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6301 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6361 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6421 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6481 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6541 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6601 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6661 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6721 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6781 ttgagataat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6841 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6901 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     6961 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7021 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7081 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7141 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7201 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7261 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7321 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7381 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7441 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7501 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7561 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7621 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7681 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7741 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7801 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7861 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7921 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     7981 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8041 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8101 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8161 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8221 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8281 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8341 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8401 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8461 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8521 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8581 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8641 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8701 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8761 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8821 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8881 aggattcttt gaaagataca gataatatgt tgactatcac aaaatacaat ccaagtgctt
     8941 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9001 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9061 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9121 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9181 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9241 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9301 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9361 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9421 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9481 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9541 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9601 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9661 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9721 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9781 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9841 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9901 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
     9961 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10021 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10081 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10141 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10201 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10261 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10321 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10381 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10441 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10501 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10561 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10621 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10681 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10741 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10801 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10861 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10921 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    10981 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11041 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11101 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11161 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11221 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11281 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11341 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11401 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11461 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11521 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11581 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11641 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11701 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11761 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11821 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11881 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    11941 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12001 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12061 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12121 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12181 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12241 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12301 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12361 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12421 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12481 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12541 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12601 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12661 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12721 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12781 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12841 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12901 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    12961 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13021 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13081 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13141 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13201 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13261 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13321 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13381 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13441 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13501 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13561 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13621 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13681 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13741 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13801 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13861 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13921 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    13981 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14041 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14101 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14161 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14221 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14281 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14341 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14401 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14461 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14521 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14581 ccaacctggc gacctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14641 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14701 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14761 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14821 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14881 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    14941 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15001 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15061 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15121 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15181 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15241 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15301 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15361 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15421 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15481 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15541 agctgcaact gttcctgtac taaccttgtt aaggttagac aaatcattct ggcttgatct
    15601 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15661 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15721 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15781 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15841 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15901 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    15961 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16021 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16081 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16141 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16201 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16261 gcgtataccg tacttctaca tctaaagttc caggaaatcc gttttccata tctgggctag
    16321 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16381 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16441 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16501 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16561 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16621 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16681 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16741 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16801 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16861 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16921 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    16981 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17041 agatgaaata caatacgaag aatatcgtaa acggaaccca aatatctttg tatggatttt
    17101 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17161 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17221 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17281 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17341 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17401 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17461 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17521 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17581 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17641 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17701 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17761 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17821 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17881 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    17941 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18001 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18061 tgaaataagt tcctaatacg atcgtaacta aggctacccc tataccaaag acttgatagg
    18121 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18181 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18241 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18301 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18361 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18421 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18481 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18541 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18601 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18661 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18721 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18781 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18841 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18901 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    18961 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19021 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19081 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19141 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19201 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19261 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19321 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19381 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19441 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19501 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19561 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19621 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19681 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19741 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19801 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19861 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19921 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    19981 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20041 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20101 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20161 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20221 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20281 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20341 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20401 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20461 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20521 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20581 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20641 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20701 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20761 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20821 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20881 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    20941 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21001 ataaatatct aatgttactt ttttaatttt tttttcgaat acgcgccatt acaccttcta
    21061 gttctaactc tttgtcttca gtgatgtacc aaccgcttgc ctgtgtccct ccggctgcct
    21121 gcggtaaata atccaatttt gttcgaaccc agtgcttttc ttctgtaaaa tagtcttcta
    21181 ccaagacctc tagatccatc cctgctgaaa gaggcaaatc ttcccgaaca gatttaaaga
    21241 taaatcgttc cttcttccca tcatacttca atattcctgt atcgataatc ggtaaagaat
    21301 aataatccag ctctttcttc accgtcaata aatgtttgaa aacttcatgg tacacttgct
    21361 ctaattggcg accttctgaa ttgttcgggt aattaaccat cactttttct ttgtctggat
    21421 catagaaggt ttggcttagt tgagtgatat aagtatgtaa tctttccatt tctgttttta
    21481 acttttctct ttgaccttct tctaccttca tatactcttt caccctgttt tctttttaga
    21541 tattttcttt cttattttac tatagttatg tgcgttaagc gatgaaaaac agcaaagatt
    21601 ggaaggatct ttgctgtttt ttcttaaaat atccagttga gtacaggatc aatctgtttc
    21661 attgtcctct tgatccgtcg taatattgat cgatgtgtct ttagcagtga tctttagctt
    21721 tttctgtcct tgttctaaca aagtatttgt tattggctgg tagtcttttt caatataaga
    21781 ttcaccagac gtttggatag tatagccact gttttccatc tcatgcaaag ataaattgcc
    21841 accgtttagg atgacttttc cttctttgct gatcaaactg tcttctagat ggaagttgct
    21901 gttttcacct gtgacgatca agttatccac attggcctca tctaatcgaa gtgagcctaa
    21961 ttcatctttc gcagttattg ttcctcctgt aaattcattg aacgataccc agccatcatg
    22021 tgtcttagta ttcacttttt tgattgatac ttcgttgata tcaattgcac tattattccc
    22081 ttcgattgtc aattcagata attcatgatc tgcagggaca acaatagtga tttgtgcact
    22141 tgtgtcttct aaactaatga ttgcacctgt tgattttttg actgcattca cagtcaacac
    22201 gccatttttc tcctttattt ctggcttttc tgtcttatca taagtcgttt ttatctcaaa
    22261 atccaatccg cgtatgaccg aaactgtttg attttctgca tggatgacga ttttgtttgc
    22321 atccttgatt ttttttgtct cagtaaccat atcgatcaac cgaggaccat tatcccaagt
    22381 cagactcttt tgagcgccta atgtataagt tgcacctgct aggaggccac cagtaagcat
    22441 cagaccaata ccggtaatca aaaaatgttt caatctcaat ttatgtgtcc tccttcaaac
    22501 gcccgctgaa tgcagatctc tttttgtaat aaaacgtcgt cccatccatt taacaaagtt
    22561 catcatgaca taaaaaagcc cttttgttcc tttcagcaac agtggcgaaa taaatactgc
    22621 gcttcctgtg gcgaacagac cgacaccgat aaagaataat ccactcagaa tcgattgtcc
    22681 aaacacactg attcctgaga tgatcatgaa taggccaaca cctaagatac tgatacataa
    22741 aacatagata ctaaaaatga gcgctagtaa agcaaccaat agtcccccaa taaccaatat
    22801 aaaccccagt gctagtggaa tgaggacagg agaagcaaat aaaccgataa tgattaacca
    22861 aatcaggcga atttgtcttt ttggattttt cactaactcg ttttctgctt gttcttcttc
    22921 tgaaaagtaa ctgattttta attttaaggc aaactgcttt ggtgtaccat attcatcaat
    22981 gatctgatct aatgtttttc cagaatctaa aaaatattcc tcataatact ggatcaaatc
    23041 aaaacgatcg tcttcaggaa tttcttgaag atgatccaca acttcattca aatattcatt
    23101 catttgtgct ctcctccaat aaccggtcta aagaatctcg aaaaacaatc cattcatcaa
    23161 taatttcttc taaatgacga cttcccattg gtgtcaacga gtaatatcga cggtttctgc
    23221 cttcaaaagg ttcatcatag gtctcaagat atccttcttt tttcaatctt ctcaaaaccg
    23281 gatatagtgt agattcactg acagagagac tttcttgtac gatttttgtt aaggcatacc
    23341 catacatatc ttccttgctt aatgctgcta gcacagtacc atcaagaaga acagttggaa
    23401 tctggatgtg cattacgtca ccttctattc tcaagtgtaa tattttattt caatctatat
    23461 tatacaacat ataatatagt ttcaaaatca gtttgtcaaa tacaaaaaaa ctgactaaaa
    23521 gatttgatcc tttttagtca gcaaaaaatg gcttctcttt gatttttatt ataacagtgg
    23581 cataatccaa tttgtccaca tccagctcag tggcatgata acgatcattg caggtatcat
    23641 gtcagctact gggaacattt tcacgttgat cattctaaaa ccggtagcga ccatcaagaa
    23701 tccgccgcaa gctttaaaat ctaagatcat gtctggtgta gtcaatggat aaataaattt
    23761 tgccaataag aacaaaatat aaaaaataat gaattgggga attgctatca cagaaacgac
    23821 ataccctaaa ttacacgcaa aaatagctgc tgtgaaaaaa tcaaggacag atttagaaat
    23881 caaaatcgta ctgtctcctg tcatcccaga atctaaactt ccatagatcc ccgtaccact
    23941 tgcacaaaat agaacaatga ccgtcactaa tgtgctgata aattcatcgt gggtcatacc
    24001 aagcttttca ctagggaaga ttttagaaat aggtacttgc atcagcgcag cccccttatt
    24061 tatcccatta cctaaatgaa caatcaaacc aatcgctgtt cctatcacta aagcaaaaat
    24121 aacagccggc atatttttca tcggcgcaat ggaatagatt cccatcccca tcgaacacac
    24181 accaaaaacc atcgtcagct gtgctttaaa atgttcactt aatttatttc ctactaatcc
    24241 gccaattagt ccgccaaaca aaacggaacc tacattgatc aaaactccag ttggcatcaa
    24301 aaaactcctc tctttttcta cgaagcgaaa aaaatcagaa aaatttctgt aattattgct
    24361 ttcgctgcaa gaaagattgt aacagacaga gaagattaca ccaattcatt ttttgtgtta
    24421 aagtattctt tattggaata agtaggagga atatgatgga tatccataaa ttcgaagttt
    24481 ttcttgattt agccgaaaca atgaatttca ccaaaactgc tgatcgacaa ttcaccaccc
    24541 aaggaaatat ttctaaacag attctttcgt tagaaacaga attagacgtc aaattattcg
    24601 aacgcgccca tagaaaaatc gaattgaccg aagctggaag cttattgctc ccatatgtaa
    24661 aaaatgtcgt agaacaatac catgctatgc aagaagtgct tgaaacctat acgaaagaca
    24721 acaatctttc attaaatatc ttaacgatac cgacaatgat caattataaa ggtttttcca
    24781 aaatcaccga gtttttgaaa cgccatcctg aatttacagt ccagttgaaa gaagtggaaa
    24841 gtgtcgaatt aacttacaat gacgccaatg aacatagtga cacgatccac tttgctagaa
    24901 gtttccaggc cccaccaagt tcgattgagt ggttgcctac tgaaacagac gattttgtag
    24961 cagttctacc taaaaatcat cctttagcta gtaaaaagaa attggatcta agcgaactga
    25021 aaaaggagaa ctttttgctc cttggcccca ctacgaactt gtatcaacct gtactagatc
    25081 tgtgtcgcga agctggattt gaaccaaaaa tctcttataa aggagcaaga atcgacttga
    25141 tcatcaatat gattgcaaat gacatgggga ttgctattgt tatggaaaaa acagtcaaga
    25201 atctattaaa agaaaatact gtgattctcc ctatttctcc aacaacagag agttatcttg
    25261 catttactcg taaagtcggt gagcattctt tggcttcaga tacattttgg gagtatttaa
    25321 atcaagaaga aaactaagcc ctacattttc tgtccatcta tccacccaaa aaaagagagc
    25381 gacgcatgac tttcgctctc tttcttgcat catgtccttg tttcaatgac acctctaaca
    25441 tttctcgtag ttcaataaga aagcagcgat ttttatgcca aaagaaccgt aagaacattt
    25501 ccctaatcac aaaaaaacga ctaaaaaccc ctaaaaacct tcaatagaat ttttaggggt
    25561 tttattcttt ttcctattgt ttacttaacc gtatcattgc tgtcgttcct actccttcga
    25621 tactgctata atcaatacgt ccatcatgca gttctagaat ttttttaacc agtgaaagtc
    25681 ctagcccatt cccagaaatt tgacgacttg tatctccttg ataaaatttt tcaaacattc
    25741 gatctctggt ctcttcattc attcctacac ctgaatcggt cacttccagt acaatctctg
    25801 tggctgtctc aaatagtttg atatgaatct gtccattgat ttgattgtac tttatggcgt
    25861 tatccatgat attcagccac acttggtaca aaaactcttc attgcctgtg taatttactg
    25921 cagcaagttc gatatccaat tctagctgtt ctttctccca tttaggctga aaaaaaacga
    25981 ttacttctct gatttgttca tctaatcgat actctttctt ttccagttgg atacgctggt
    26041 tttccagttt gttcaatttt aagatgttct ctgttaattg tgaaagctgc gtgatacttt
    26101 cgataatccg atataaaaat atttggcgtt cttcgtcaga gagatttggg gcttgtaaca
    26161 attgaacata tccttggatt gttgctagtg gtgttttgaa ttcatgggat acactggaca
    26221 caaagtcatt cctcagtgtc tcgatactat tgagttcttg gaccataacg ttgaaatctt
    26281 tatacagttg ctgtacttct gctactttct gctgttcatc cattcgaatc gaaaaatctc
    26341 ccgttgctac cagactcata tttgtcccta gcgcactgat tggatgcagg atacgccgcc
    26401 caacaaaaat cgatattcct gtacccacga ccatactgaa ccccgcaaag acgaataacg
    26461 ggtataacgt tggttttcca gccgattcag caggcgagat agaatgtcga gacaataaga
    26521 aaataaaacc gagaaaacac agcagtgtca ctaagatcga aatgaagaca atactagcaa
    26581 aatagatcca taattgtgaa aatagctttt ttttcatctt taaataaccg ccttatagcc
    26641 tagtccccgt actgtgatga tttgaaaatc ttccgtcttt tcaaaacggg tccttaaacg
    26701 tttgatatgt acatcgatcg ttcgttcttc tgtattcgta tctaatcccc aaatatcatc
    26761 catcaactgt tggcgagtga agatcttgtt gggataagac agtaatttat acagaagcag
    26821 aaattctttt tgaggcagaa tcaacgaatg ctgatcttgt aacactgtat aagcatctag
    26881 ctctaatttt gtttgtccga ttatcaagct actgctgtaa ttgatctttg aacgtcgaag
    26941 caatgcttcc acacgtagga tcatctcatt gacatcgatg ggttttgtca tataatcatc
    27001 tgctcctaat ttgaacccgc gtttcttatc ctcaaaatcg gcctttgccg taatcattag
    27061 aacaggtgtg tcaaatctgg tgctacgtaa tgagtctagc aagtcatagc catccatttt
    27121 cggcatcatg atatctgtaa tgattaattc tatacgtgtc gcttctagtt ttttcagcgc
    27181 atcctctcca ttcaatgcgg tgatgactga aaagcccgct cgttccaaca cagaacggta
    27241 aagcaactgt aattgcggat catcttctac tactaaaata cgattcattg atctccctct
    27301 gttttttgaa aaatttctag attttttctt gtagttcatc ttcagttaac atttatttcg
    27361 tatactacga tcattgttct gatatactta ttagtatacc gaatatttca atacgggaga
    27421 agggtgatta tgcttcaatt aaaagatata aaaaaatatt ataaagtcgg cgaaaccacc
    27481 accaaagcat tggatggtgt gtctgtcgct ttcagacaaa aagaattcgt agcgatttta
    27541 ggtcctagtg gttctggtaa aacgactatg ctgaatgtga ttggtggact agataattat
    27601 gattccggtg atatggtaat caacggaaag tctactaaag actttaagga cagtgactgg
    27661 gatgcctacc gaaacaactc tatcggtttc atttttcaaa gttacaactt gattggtcat
    27721 ctaggcatta ttgaaaacgt tgaacttggg atgacattaa gtggtgtttc taaagacgaa
    27781 aaacgaaaac gggcagaaga tgcattgcac cgagtcggct tgacaaatca tatgcataaa
    27841 aagccaaatc agttatctgg tggacaaatg cagcgagtgg ccatcgctcg agcactagcc
    27901 aatgacccag atatcctttt gtgcgatgaa ccaaccggag cgttagatac agaaacaagt
    27961 atccagatca tggaattgat ccaagagtta tcgaaagaaa aattagtgat catggtcaca
    28021 cataatcctg aactggccca tcaatacgca gatcgaatca ttgaattttc tgatggtaag
    28081 atcttaacag attcccatcc tcacatcgaa cgaccaaaag atgatcaatt taacttacgc
    28141 agaacaaaga tgagtttttg gacagcgtta aagctttcct tcaacaatat ccgaacaaaa
    28201 aaaggacgta ctttcttgac ctcctttgct tccagtattg ggatcatcgg aatcgcgatc
    28261 gttctgtccc tttcttctgg attccaaaaa caaatcgata acacgcaagc tgaaacgatg
    28321 gcgaaatttc cgattactat ttctaaggta acgactaacc agactcgtga cgatgccgga
    28381 ttgggtgcaa gcaaagcgga ttatccagac agcaaaacga ttactgcaaa agtcagcgaa
    28441 gaagataaag cacagcacac gaataagatt gatcaaacat acgtcgatta cgtaacagac
    28501 atcgatccta acttaagtaa taatatcgga tttacccgta ctacaggaat caatctttta
    28561 cgtgatgtaa atggcaaagt acagcctgtc agcttctcga atcaaaatcc tgatacggaa
    28621 tctctttctt tatctagtac gatgtcagca atgacaggtg tgggagtgtc aagtttccct
    28681 acacagcttg acactagcaa agaaaacttt ttaaaagaca attattcttt acttgccgga
    28741 agttatccag cctcagcaac agatgtcgtg ctgatcgtag acggcaataa taatactaat
    28801 atcaacgcat tgaagaatct tggatttgat gtgaaagaag acgaaaaact tgattttgat
    28861 gatatcgttg gtacaacatt caaattagta aacaacaata cttactatac aaaacttcca
    28921 actgggaatt ttatccctaa tacagattat gatgcgatgt atcagaatgc ttctgatgaa
    28981 ttgaagatct cggggatcct acgcgtgaaa tcttcttcta caatgaatct cttatctcca
    29041 ggaatcgctt atagcgatca attaacgact caaatcgtca acgaaaacaa agaatcagaa
    29101 atcgtcaaag ctcaaagaga cagtggtgta aatgtcttaa ctactgaaaa agttgacgaa
    29161 aatgccaaac aaacattgct tagttattta ggtggagaca gcttgccttc aagtatcatg
    29221 atttatccga acaattttga agacaaagaa aagatcttgg attatctaga tgattacaat
    29281 aaaggcaaat cagatgaaga taaaatcatt tatactgatt tagctggaac gatgactgaa
    29341 ctgactggcg gattgatgga tgctatcact tatgtcctta ttgcttttgc agggatctct
    29401 ttagtaacaa gtatgatcat gatcagtatc attacttata catctgtgat tgaacggaca
    29461 aaagaaatcg gtgtactaaa agctttaggt gcacgtaaaa aagacatcac tcgcgtattt
    29521 gacgcagaaa cgtgtatttt aggtatttct tctgggatat taggtgtgtt tattgcatgg
    29581 ctagctacgt tccctatcaa tagcatctta tacaacatga cggatctaaa aaatgttgct
    29641 caactaaatc ctgtccatgc gattattcta gtgatcgtct ctactatatt gacgatgctt
    29701 ggcggtcatc ttcctgcacg aatggcagcg aaaaaagacg ccgctatcgc tttgcgtgca
    29761 gaataaggtt gtgacaacca catttagatt tgagtcataa gagcgctgaa atagtagaaa
    29821 acagcaagaa acgaggaagt aatatgttcc ttgtttcgcg ctgttttttt taattatttt
    29881 ttataattct ggtatacttt tctttccttt gtaccacaac tcttctggcg tatacctgtc
    29941 ttttccattt atgaatactg ttccatctgc ttcaatcgtt acttcagtcc caatccacat
    30001 attacgagaa agtgcgagaa gattttttct ttttcctctt cctttttttc caaattgcaa
    30061 ggcatcggtt atctcttctg tccaatgaga tttcacgtat tttacttctc cgttttcaaa
    30121 tgtaactttc aaaatattcg ctatattttc cacttttact gctttaggaa atgactcctc
    30181 catttttttc actttccttc ctccaacact aagtatgcgc tttcttttag tgataagtat
    30241 atcatagttt attctattat caaaaaaacg catccattcc attgtagaat actttaccga
    30301 acaagttgaa ttgttttcct ctttttcacc ttttactata gataggtaaa gacaactaag
    30361 gaggaatctc tcatgacaga tcaaccgtat gatttaagaa aagtcatcga agaactaaaa
    30421 acacttccag gtcaatatca cgaaacagat attgaaatcg atcctgaagc agaattatct
    30481 ggtgtttacc gttatatcgg tgctggcggt acagtaaaac gtccgactca agaaggacca
    30541 gctatgactt tcaacaatat caaaggtttc cccggcacac gtgtgaacat cggaacaatg
    30601 gctagtcgta aacgtgtagg tgaaattttg caccatgatt atcgaacact tggtcgtttc
    30661 ttgaaagacg ctgtagaaaa tccaatcaaa cctgtgaaag tagacaaagc agacgctcca
    30721 gcacaagaag tcgttcatct agctaccgat gcagacttcg atattcgcaa cattttgcct
    30781 gcacctacaa atactcctga agatgctggt ccatacatca caatgggtgt agtcttagga
    30841 tcagatccag aaaaaacaat gagcgatgta acgatccatc gtatggttct agaagataaa
    30901 gatacgatcg gtatgtacat catgcctggc ggacgccaca ttggtcactt ccaaaaacaa
    30961 ttcgaagcct tgaataaacc aatgcctatc actatcaata ttggtttaga tccagcaatt
    31021 gccattggca cgactttcga accacctacc actccacttg gctacaatga actatggtgt
    31081 gcaggagctt tgcgtaacga acctattcaa atcgttgatg gtatagcagt cgatgaagtg
    31141 ggtattgctc gtgctgaatt catcattgaa gcagaaatca tgccaaatga aacaatgcaa
    31201 gaagatatca acacgaactc aggaaaagca atgccagaat ttcctggcta tactggggat
    31261 gctaatccag ctgtacaagt agtgaaagtc aaagcagtta ctcaccgaaa agacaatcca
    31321 atcatgcaaa caacgattgg tcccagtgaa gaacacgtat ctatggccgg tattcctaca
    31381 gaagcaagta ttcttgcctt ggtcgataaa gctattccag gaaaagtagt aaatgtctac
    31441 aatcctcctg caggtggtgg taaattgatg accatcatgc aaatccgtaa agaaaacgaa
    31501 gcggatgaag gtattcagcg tcaagcagct attcttgctt tatcagcctt caaagaattg
    31561 aagacagtca ttttagtcga tgaagatgtt gatattttcg acatgaacga tgtagtttgg
    31621 actttcaaca ctcgtttcca aggagataaa gatatcatcg tgatcccagg aatgaggaac
    31681 catccattgg acccatcaga attaccagaa tataatcctg gctctattcg tttccgtggg
    31741 ataagtgcaa aaactatctt tgacggtaca gttcctttcg atatgaaaga acattttgta
    31801 cgtgcaaaat tccaagaagt agaagattgg gaaaaatatt tgaaataatg aatgatctgc
    31861 aactgttttg acatcataaa aaggagtgcg gaaaatggaa caattggcac aacggaagaa
    31921 aaaaatcgtg attggtgtaa gtggcgcttc aggcaccatt tatgccatca acctagtgaa
    31981 gaaactaaaa gaatatccga tgatagaaac acacggaatc atcagtccat gggcaaaaca
    32041 gaacttaaag ctggaatccg acttatcact aactgaattc gaaagcctgt ttgattacca
    32101 ttattcaaac aaagatttag gtgctgctgt cgccagcgga tcatttttga ccgatggaat
    32161 gatcatcgta ccggcaagta tgaaaacagt cgcaggtatc tctgttggtt taggcgacaa
    32221 cctgattgca cgtgctgctg atgtaacaat gaaggagcaa cgaaagttag tcattgttcc
    32281 tcgtgaaacg ccattaaatg ccattcactt ggaaaacttg actaaattag cacgactagg
    32341 tgtgcaaatc ataccaccaa ttcctgcttt ttataatcag cctcaaacga tccaagatct
    32401 tgttaatcac catacaatga aacttttgga tcaatttgaa attgtaaatg agcattcaaa
    32461 acgttggcag ggtgattaat atggaaaaag aaacgatagt tgaagtaacc aaactaggtt
    32521 tcacgatcca agctgctgtt aaagtaatcg gtaaagactt attgatcact ttaacaggcg
    32581 gagatacacc acatatcgga acagtcacta cattttctcg tgatacagag atgcaaactg
    32641 tacgtttccc tagccacgat ggtcggttcc ataaagacga tgtcttagcc gaaaaaatag
    32701 ctgtgatcat caaaactgcc gttccgggaa gttgtacgat cacttccggt atccatgttg
    32761 accatatctc caatgcacaa atcaacgcct cttttccgat gtcagaagag ttaggttaca
    32821 agattttaga ttggttggaa aaaatagatt tccatatcaa ataaccagtt tattataaag
    32881 atggtgaaaa accattatga aagaagaggt tgtgaaatca gcgttatgac ctgagtatga
    32941 gagaaaaaaa tggaaaaata gcttctcata ttttgccgtt tttgagctaa taccgaggga
    33001 cagctgattg aacgccgttt atcaagaggt tgtgataaag ccgcttcgtt tcgagtagta
    33061 agaaaaattt tgtaaacatg ggtttccaca tttttgcata aatttgactt actaccgaga
    33121 aactggcttt tgaacaccgt ttatcaagag gttgtgacac gtcttttgtc acaacctctt
    33181 ttatttttgc ttcactgtaa ctgctacaat atcaccgatt tcttttccga tgtttttacg
    33241 aatatctttt cgtagtccaa ggatgtagca gactgaacca tctggatttt tcacgcccat
    33301 attcacgata ctgccttcat acggcacatt gtcaaaagtt gcttggactt taactcttcc
    33361 agcaccgaat tcttttctta tatcatacgg aaaaataata tatgccccgc ccttgccgac
    33421 ttcggaagca tagatttttg attcaaaaga ataaatttcc ctcattttat ttctcctatt
    33481 ttttacatct agtatagcat agcaaaaaaa agaaggaact gtgacaaaag tcttgtcgca
    33541 gttccttttt tcgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatag
    33601 tcaacaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttaaacggca
    33661 ggctcacaac ctcttcctta aacggtgttc aaaagcttgc atctacggca ctaagcccaa
    33721 tcagatgaaa aatatggagt gctattttta tctgattgtt cttactgctt gatgcagagc
    33781 agctttttca caacctccta catggtgttc aaaatattat tcgccttttg cttctttcac
    33841 taattttgcg aatgcttgtt gttggaattc agcagctaag gctttcgtac gttgttcttg
    33901 ctctgctttg ctcatttttg gattttcagt cattgctttt accataccgt cttgaacata
    33961 agcttttgct tcactttcca tcgtactttg ctgtttcttc actaattcag ctaacttggc
    34021 tgcttggtcc gtacttaatt ctacccatgt ctctgggatt ttgaatgtat tcactcggct
    34081 gtcatattcg tgattgttat cagcaatatt gaaccagaat ttatacatgt cgtttttgta
    34141 ttcccaataa gtagttttcg tcactttttc agcatcgccg tctgtctttt ctactttatt
    34201 tgtttcgtta tccgtaccag ttgcttttgg tttttcttgt ttttcatttg ttcggtaaag
    34261 atacactttt tcttttccat tccctaaagc tttatacaaa agcatatctg ctccttggga
    34321 atcagcactg gaaacaattt tttctgtttt tgtttccgtt actttttcca ttccaaagtg
    34381 attggacaaa ttagcgacga tcaaacttag tgaagcgacg aagattagtc cgaaaatcaa
    34441 agataaaaag gtttgccatg tttttttagc gaacacgttc gttaaagcaa aagcaagcaa
    34501 gctgacaatc aaaactccaa taatcatgca ttatcccccg ctttcattag aacaggttct
    34561 tttttcttca ataaaaaggc aataataaag ccaagaatcc caaagatgat cgctacgata
    34621 aacgcggcat ggtaacctga cagtgtggca ttgattgcct gatctttata agctaatggc
    34681 gtattatcca atacagattt gcttgggata tttgcttttg tcacattcgt caacacactg
    34741 atcaaaatag ctgtaccaat cgagcttgcg atttgacgga atgtattatt taccgctgtc
    34801 ccatgactga tcaagttatt tggcaatgca ttcataccag aagtcgttac cggcatcatg
    34861 accatggaga tcccaaacat acggatcgca taaaggatca cgatatataa aactggagtc
    34921 gtttttgtca agaaagcaaa tggtgcagtt gccgctgtca aaatgaacat ccctgtgatg
    34981 gctaaacgtt tcgcgccgta tttatcgaaa atcgcacccg tcacaggcat catagccccc
    35041 atgatcaatg cacccggcaa caacatcaag ccagaatgga atgctgattc tccacgaata
    35101 ttttgaatat acaatggtac gaccatttct gcccctacca tcgccatatt tgttacacca
    35161 gctaaaacag ctgcaatcgt gaatgtagat gatttgaata cacgaagttc caagaatgga
    35221 tgttccaaat gtaattgacg ccatgagaac aaggcgatga aaatcacacc aacaactaag
    35281 aaaccaatta ctttcaagct tccccagccg tcatctccta cactagaaaa tccgtaaagt
    35341 aaactaccga atccaatcgt tgataaaata gcggataaaa tatcaatgct aggatttgac
    35401 aatggaataa cacttttcat taggaagaat gctaaaacca agacaagaac aacgattggc
    35461 agtaccatac caaacaagtc acgccaatgg tagttatcaa tgatataacc agataatgta
    35521 gggcctaaag caggagcaag tccaataacg atccctgaca tccccattgc tgatcctctt
    35581 ttttctggcg ggaaaataga tagcatgata ttttgcaata atggcatcga tactcccaca
    35641 ccgcaagctt ggatcaaacg accgattagt aatgtactaa aattaggtgc tgtaaagcaa
    35701 gtaatcgttc cgatcaaaaa gatcgtcatg gcagaaagat ataaatttct tgaactgaat
    35761 ttattgatca gccatgctgt tattgggatc atgatcccgt tgactagcaa gaaaccagtc
    35821 gttaaccatt gcacatcaga cgctgaaata tcgaaagctt tcatcaatgt aggaaaagct
    35881 gtcgtcaata aggtctgatt caaaacggta caaaatgtac caattaataa gacaacaact
    35941 aaaagtgtcc gattatatgg ttttccgtaa atatctacgg gttggttgtt actcatattt
    36001 ctccttcttt ctgaattttt tctgaaacta ctagaatact ttagtcctaa attcgctatt
    36061 tgtcaaccta cttgacacat aagttaatac ggatataatt aagggaagca agaggacaaa
    36121 aggagtgaca aaaatggttg gggcttccct taaacgtttg ttggaaaatg atcaattggt
    36181 tgttggcatc agtgaattaa gtgatatcgt aggtgtgtct ccacgtcaat taaggtattg
    36241 ggaacaaaag ggattcattc gttcgatcga agctgatgcg aattgtccta gaaaatatcg
    36301 tttacctaca gtcataaaag tagaactgat caaaaaatat ttagatgacg gatttacttt
    36361 gtcaaaagct gcagaaaagg cagaaatccg ccaaaagaaa atgcaccatg tccgtaaagt
    36421 tttttcaaaa gcaatcaaag atatcgaatt gatcgacgaa cgctatacga ttttacaaat
    36481 cggtaacttc aaagaagaga atgctaagat gtacatcatt catgacaacg agacagacga
    36541 attaacttac aagctccttc ctgtcgatga aaaagctgac tacgaacagt tgttaaaaga
    36601 actctaaacg ccactaatct cctaagtacc aagcttttta ataagtagat gctacataaa
    36661 tacttgaaaa aaggcctgtt tgaacataag aatatccgaa cggtgatctg cgtttctttc
    36721 atatagaaag gaattctcca tcactgttcg gatatttttt tatttttttc ggttactttc
    36781 ttctaataca tctgttagcc aatttgcata ttctgtttcc cgattgatcg catgcgctaa
    36841 aatcaggtag tgtccgtaat tttcatgttt ttctttttca gtaggaaaga tcgttttttt
    36901 tcgctctaat agatgaagtc gtttttcttc atgaaggtca cgctgctgct gaataatttc
    36961 tactaatgcc ggatcattca cgtctttgac gaaatacaat ttcagaacaa attcgtctcg
    37021 attaaccggt agttcattgg tgggtgtatg aatccactct gccaattgtt ccttcccttc
    37081 ttctgttaat tcataaactt tcttttccaa tttttcacct gtgatctcta cttgatgctg
    37141 aatgattcct tgctcttcca tttttgcaag ttcaggataa atctgactat gttttgcttg
    37201 ccaaaattct cctatttcat ttttgaacga ttgattcaat tcatatcctg tcatcttctt
    37261 cttacttaat aaccctaaca aaatatatcg aagtgtattt tttcttgcca tcagttttcc
    37321 ctccttgaca aatctttaaa tccattctat cataaatgag taatcgaaaa catgtagttt
    37381 attacatgtt ttaatttata aggagatgct taaacatgaa agaatttaaa gaattggaag
    37441 attttattgg aactcacttt atctatacgt atgacaatgg ttgggaatat gaatggtatg
    37501 ccaaaaatga ccatactgtt gattaccgta tccatggcgg aatggtcaaa ggccgttggg
    37561 taaaagacca agaagctgat atcgttaaat tgacggacgg cgtattcaaa atcacttgga
    37621 cagagccaac agggacagac gtggcattgg actttatgcc aaatgaaaag aaacttcacg
    37681 gaatgatctt tttccctaaa tgggttgaag aacatccaga aatcactgtt actttccaaa
    37741 acgaacatat cgcagaaatg gaagcagctc gtgaaaaata tgaaacttat ccaaaattac
    37801 ttgttcctga atttgctacg atcacttata tgggtgatgc aggtatagac aatgaagatg
    37861 ttattagcga agaaccatac gatggaatgc cagatgatat ccgtagcgga aaatacttcg
    37921 atgaaaatta taaacgagta aaataaggtt gtgaaagaga ggttgtgaaa tcagcgttct
    37981 gacctgagta ttagagaaag aaacggaaaa atagttcctc atgttttgcc gttttttgag
    38041 ctaataccga gggtcagctg attgaacacc gtttatcaag aggttgtgaa atcagcgtta
    38101 tgctttgagt aataagaacg ctagttagaa aatagctttt catattttct aggctagtgg
    38161 gcttattacc gagaagcagc cgtttgaaca ccgtttatca agaggttgtg aaatcagcgt
    38221 tttgacctga gtattagaga aacaaatgga aaaatagttc ctcatatttt gccgtttttt
    38281 gagctaatac cgagggtcag ctgattgaac accgtttatc aaagattgta acaactgcat
    38341 attcacacag ttaaattagt gacttcaaaa tgaaataatg ctcccagcaa aaaattaggc
    38401 tgggagcatt atttcgttat tctgttcatc aaaaatcagc gttttgattc catttcatca
    38461 caagttcctc ctcatttact gcttcatctt tttgctgtgt ttgaattgta aaaccttgtg
    38521 aaagatagaa atgaatagct gattgatttt tttgatagac tgtcaaagtc agtgttttat
    38581 tggtttcttt tatttttttc attagttgcg taccgattct ttgttgccgg tattcccttt
    38641 tcacaaagat tcctgcgata aactgatcaa tcaccccagc aaaaccaata atttcttgat
    38701 ctatttctgc taagtaaatc actgcatctg gtaaagcttt cctaacagat tctacttgat
    38761 tctcccaata agttgatgga atgaattcat gagaatccaa gttagcatgc agccaaatcg
    38821 atagaacaga ttccagttct ttggcagaca attttgttgt tgtgcggatc attttttctc
    38881 tccttactta ttcattgatt ttaaaataaa aaccattttt ttgaagctct tttacaagtt
    38941 ttcgcccctc aaaaaagtat atcttctctt gttttacttt tctaatacca aaagaaacag
    39001 caactttagg atcatcagct gaaacgattc gataagttcc accaggcaat tgatccagct
    39061 tgactttccc atgatggtcg ctttgtactg aaaacggcac accatttcta gtaagtgttt
    39121 ttttcttatg tttcaaaatg aatgaactac catttaccgg tttccctgac gaatcactaa
    39181 taataaatga aaaatcaagc acttttttac ttaatctata ctgatagatt agtcgtccga
    39241 taaacactaa cagtatgact acagctatta tcgttcctgc aagaataaac cattgccgca
    39301 tggttctttc ctgatttcct tttccttctt ctttttttac cgattcgtta taaggaatcc
    39361 gatgtcctgt cactagcaaa cgatgcgtgt tgatcatata cggcgtacat gtaagcaagg
    39421 taactaagtc ttctccttct gaaatcgtta gaacagacgt gtcatctggc agtaccactt
    39481 gtatattttt aacttgataa gccaatttct cccccaaaac agtcaaaata aacgtgtctc
    39541 cgttttctaa acgatctaat tcggtaaata accgtcgatt aggtaaaccg ctgtgagcag
    39601 agatcactga atgcgtcgaa tttccaccaa gcgggaaaga ggttccttgc aagacgcctg
    39661 ctccattttc aaggatccta ccattgagcc gatcataaag tgaaatattt aattggatct
    39721 taggtatatt gatacttccg atcagccatt cctctaattg gctggatgtt gtactttcat
    39781 ttctgttctt ttcattgaaa ggatcagtac caggattgaa tccttggtca gttaatcgtt
    39841 ggtttttctc ctccattttc tttcgctgca cttcaatttc agctgcggtc cgtttttgta
    39901 cttgttcgat acgtttttga tcaataaaac tatttaaaga atccacataa aatggatata
    39961 aagcgatcag taaccctacg acgatacata ttaataaaaa caaacgaaac aaaagtcgac
    40021 gagactgttt ttgcttactc tgcttcacat gtgtcctccc ttgcaagttt ttttgttctt
    40081 tcttttatta tatgggttca ggaaaaaaat agaaaatgaa agccgatgag gttgtgaaaa
    40141 agctgtcctg catcaagtaa taagtacagc ccaaaaaaat agctactcat attttttgtt
    40201 gggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa gaggttgtga
    40261 aaactgcgtt atgctttgag taataagagc gctagtcaga aaatagcttc tcatattttc
    40321 taggctagtg agcttattac cgagaagcag ctgattgaac accgtttacc gagaggtcat
    40381 gaaaaagcta ctttgtttcg agtattaaga aaaattctgt ttcaaaacaa ggagatgcaa
    40441 caaaacttat gtcgcatcct ctctccttag aactttcact tatgtttttc ttgtaaaatt
    40501 cataaatatt ttcatgaatt ttctaatttc ttgcctacct ctcgcatagg atatatgatg
    40561 aatttatcta aagtttgcaa aggagttcga caattgaaaa aatatggccc atttttagca
    40621 atttttttca ttgctttggc atttctcatg cctcatttat tcacacataa actaattttg
    40681 ggtgctgatg ccccattcca ttacaatcgg ttctatgatg ccgcgcaaca aattaaatat
    40741 ggggatttcc attattttcc ttccctttat ggattccagc aaagtggcag gatcgtgaat
    40801 gccgtttacg gcccattttt cgcttattta caaggattga tcgtgttgct tgcaggtagc
    40861 tggtttcatt atcagattct ttcaaatttc attctttatc ttatttcagg attttctatg
    40921 ctgattttac tgagatatgt caatgttcga aattgggtaa gtgtaccagt agccgtttta
    40981 ttcatgagta cgtattctat tcaatactgg tctatcaacc aaggattcac tagctggggg
    41041 acttgttttt atcctttatg tatgatccct attgctgatt tcgtccttca taaaaagttc
    41101 cctgttatca aagtcgctgt ctcagtggga cttatgacac agatccattt actgtcttcg
    41161 atcatgttag ctttgatgta tatccctttt tatctttatt acttttttaa ccagactgaa
    41221 aaaggaaaag cactgcttca actgattaag tcggtcttgc tttattttgt tctgacagca
    41281 aatatttggg caggattagc tattgtttac agtgggaata agattcttcc tccctttgtt
    41341 aacagagaaa tgagtcaaaa ggcgatagat ctggatggca gttattggct ccattaccct
    41401 aaggcctttc ctatattgat attatcaggt tttttgcttt ttattttctt tcaacaaaag
    41461 aacaccttat tccaaaaaat cgtgttgtac acaagtcttg ttttcttttt tctttctact
    41521 agcttgattc catggacgac attgatcgaa aaaaatgtgc cttttattag tttgatccag
    41581 tttccatttc gatttttcgt gccatttacg ctattgcttc tactttacct tgccctttca
    41641 ctgaaccagt ggtctgaaca aaagtggttc cgtcttttaa gtatatttgg cattcttatt
    41701 ttttcagcac agacgatcca gaacttatac cagcatctgg aaaagtggga aaatgaaaca
    41761 tttgtcagtc gacatactta tctattcgat acgccggaag aagcaaggaa aacattcttc
    41821 agcaaagata tgtctaccag cttgttcgtt ttccaaaaaa ccacacctga ctatctgcca
    41881 atctataagg agacaaaaga caataaatac gaccggtata acgaagaaat actagaaaac
    41941 gaaacaactt ttataaaaac acatccaggc ggtcttttga caatcaagtg gaaaaacact
    42001 ggggacaaaa ctgttcatat ccctgttatc gtctacgatc ggactgtgct acagcaaaat
    42061 ggaaaaacat tgactgatta tgaagtaaca gatatcggga ccccaatcgt gaagcagcaa
    42121 aaaggaatca acgagctcac gcttcattat caaacgccaa tctatttcta tttcattctt
    42181 tctcttactt tgattggctg gtttaccttg ctatgtttat ttatttatca tagatacaag
    42241 cttttacgtg cttaggcaaa agggataaat acatccgatc aaaaaactct ccaaacggta
    42301 taatcgtttg gagagtttta ttttaatcag tttttttatg ttatatgtta tattttttat
    42361 tcgttcacat ctaattcgat ttccaccccg tagtgatcac tgatgattgg actatcgatc
    42421 ccatcaaaaa tccgttgata ttttttaacc tgcattcctt cagggacgaa aataaaatct
    42481 atacgcaatt tttctgtatt ttgttcccaa ccatctattt tcttttccac agtagctgct
    42541 tttccttttt cattcgcgac aacatacgaa tcttgaagaa gaaggcaact ttctcttaca
    42601 gtataataac cttgagagtc tactggatta ttgaaatcac ctaggaaaaa aagtggtacg
    42661 cgagtttcca aaaagtagtt ttccaagttt ttccactcaa aataaaagcc acttttttta
    42721 tctgtccacc aagaaaagtg gcagctcgag acaaaaatgt cctgatcatc tatctttgtt
    42781 ttcccaatca agatcttgcg tgtctgataa tcttctttct tttgaaattg ggagacggta
    42841 aggacctgac tttccaatgt ttttttcgta agcagtgcat ttccttcttc atatttatca
    42901 taaccgatgt ggctcatttc ccaactccaa aaatactctt ttccgtgttc ctttaaaagc
    42961 tgaactaaac ggtaggcaaa attatcttta cggataggta cttgattctt taccggacag
    43021 aacttcatta gcttcgcagg ttctacttct tcagattcca gtaattggtt gatttcttgt
    43081 aaagcaatca cttcataatc ttctaacagg atcttatctg ctaattcttg caattttttc
    43141 tctggttctg gctcaagcca gctatgcgtg ttgagtgtca tgaatttcat tggttttctc
    43201 cttgtcttca tttcgaaaca aaagggttgt gacaaaattt ttgtcacaac ctctccagca
    43261 aatagtgttc tcctgctaac tcctcagtaa taagctcaat ggaccaggag ctattttttg
    43321 tttggctgtt tttatcactt ggtgcagggc agctttttca cgacctctcc actaaacagt
    43381 attcaaactg ctgcttctcg gtaataagcc caaccaaacg aaaaatagga ggagctattt
    43441 ttgtttggct gttcttatga cttgatgcag gacagctttt tcacaacctc tccttacatt
    43501 ttaatggagc cgatcacttt accagctgtc actgtaccta attgcttgat cactacttct
    43561 tctactttat cgtttgtaaa tacgatcatg atcgttggat ctttttcttc gttttggatc
    43621 acatcaagat ccatctcagc taatttcgtt cctgttccta cttcttggcc ttctttgaca
    43681 gcaatctcaa aagctggctg attcatttgg actgtatcga ttcccatatg gatcaaaact
    43741 tcgataccat ctttcgtttc cagcccaatt gcatgtttgc tagggaaaat acttgtcact
    43801 tttccagcga tcggcgcaac aacttctctt gatgttggaa taacagcaaa gccgtcaccc
    43861 atcatttttt gtgagaatac cggatctttt acttcagata aaggaatcac ttctccagaa
    43921 gctgcggaaa ctagttcttg ttctatacct aagtattgct tactacctgc ttgtccattt
    43981 cctgtagaaa ccatttctgg ctctggaatc tctgctcctg attgcagcag atcttcgata
    44041 tctgatttca agacatctgc ttttggtcca taaactgctt ggactccatt gtctttgacg
    44101 atcaatccca tcgcccctgc tcgtttccag gcttcttcag tccctacttt ttctcgatca
    44161 tttacactta cgcgtaaccg agtcatacaa gcatcgacat ctttgatatt ttcttttcct
    44221 cccagcaaat gaatgatctg gaccacttgc tgatctgctg ttcctgttcc gctgttagat
    44281 acaacggttt cttcactgtc attgtcgtaa tttccgttac gtcccggtgt agcaaagttg
    44341 aattttttga tcaagaaatt tgctaagaaa tacgtaacaa caccaaaagc gatcacacac
    44401 aacacgaagt tgataagatc tccacctaga cccgctttga tagctaacgg tgtccgagtc
    44461 agcaattcga tattcccaaa tgaatggacc cgcaatggca agatatctgc catcgcaaat
    44521 gcagcccctt ggatcactga ataaatgaca tataatggca cagcagcaaa cataaacatg
    44581 aactccaatg gttctgttac acctgttaga aagacagcca acgctgctga aaaatacatt
    44641 gatttatatc ttgctttctt atcaggatcg acattacgat acatcgcaaa agccattccc
    44701 atcaagatac ctgaagaacc aatcatttgt cctactttga aacgagccgg cgtccagtta
    44761 tgcaacacaa actcatattg cgacatatcc cctgcacctt taagattcac taaatcagtg
    44821 gcccaagcca accataacgg atcttgccca aatacttgtg tccccgcctg tgcgcctgat
    44881 aaaatctcat acgatccgcc aagctgtgta tagttgatag gaatcgtcag catatgatgc
    44941 aatccaaatg gcaatagtaa acgttctaat gttccaaata aaaacggtgc tagaatcggt
    45001 gcgctttctt gtgattctgc aatccataaa ccaaaattat tgattccagc ctgtacgttt
    45061 ggccacacca cagcaagaat caatgccacg attgttgacc ataagataac aacaaacgga
    45121 acaaagcgct ttccattgaa aaatgacaaa gcatccggca attttctata attatagtac
    45181 ttattataag ccattgcccc gacaaatcca gcgatgatcc ctacgaatac tcccatattc
    45241 aaagctggtg cttccaatac acttgtaaaa aatcctttaa ccatgatctt cgtaccgaac
    45301 agagtgtggg taaatgcatc ttcattcgca agcatgtcac ttgtgacacc aaagatagcc
    45361 ccagtgatcc gattgattaa aataaatgag atcccagctg caaatgctcc gcctgctctt
    45421 tcttttgccc aacttcctcc tattgctaaa gcaaataaaa tatgaagatt accaataatt
    45481 gcccaaccaa tattttcaat tactccgccc gttgtgacaa gcggcgtcca atcaggattg
    45541 atcaagggaa ttgatttccc tatactgatc atcaacccag ctgccggcat aactgctacc
    45601 acaaccatta aagcctttcc aaatttttgc caaaactcaa aactaaatat ctttttcatt
    45661 ccgtttagct cctttacgca atcggtttcg ttaaacgtat tatagcgtgc gttttctttt
    45721 ttgtaaatgc tttatttttt atttttctgt ctataccatt ctataaaacc tataatacgt
    45781 gtgttccctt ctataatttt ctcctaaaaa tttatttttt taagcttttt tctatgaaat
    45841 attttctttt gtctattgac ttgtgcaatc ggttgcgttt attatgaacg tataaaacag
    45901 ctcattagga ggagaaaaat gaaacagatc aaacgactat ttgctattga tccatggaaa
    45961 atcagcacga cacaattaga taaaaaaaat cttcgactgc aagaatcttt aacaagtatt
    46021 ggaaacggtt atatggggat gcgcgggaac tttgaagaaa cttactcagg ggaccaccat
    46081 caaggtactt atttggcggg tgtctggtat cccgataaga cacgtgtcgg ctggtggaaa
    46141 aatggttatc cagaatactt tggaaaagtg atcaacgcct tgaactttat cgcgatagat
    46201 atttcgatca acgatcagct gatcgacttg gcttctttgg aaccagaaga cttttactgg
    46261 gaattagata tgaaaaatgg tgtattatct cgtacattca caatcactac ttctactaat
    46321 aaagtacgca tttccttcga acgttttctg agcattgtca aaaaagaatc agcttatatc
    46381 tatttaacta tagaaatgct tgaaggtagc ggaacagtca aactcgtatc aaaattggat
    46441 ggaaatgttc aaaacgaaga cagcaactat gatgaacatt tttggaatga gattgatcgt
    46501 ggaactatag aagaaatcgg ctatttgacg actaaaacta ttccaaacaa ctttgggatt
    46561 gaacaattta ctgttactgc agcaatgcgc catttcgtta atggaaaatc agtcatccct
    46621 gactatcacg aagatacttt agcattacat ggttgcttgt ctgcagaatt atctcaaggt
    46681 gagacatggt cagtggaaaa agaaattatc gttcttacta gtagagatat tccagaagcg
    46741 cagcaagcag aaaaagctgt ctcagaatta acgaaccaga caaacaatta tatccaagca
    46801 aaagaagagc aaacagcggc ttgggcagaa cgatggaagc ttgccgatgt ggtcatcgaa
    46861 ggagacgaag aagcacaaca aggtattcga ttcaatttgt tccaactatt ctctacttac
    46921 tacggcgaag atgaacgatt gaatatcggc cccaaaggat tcacaggaga aaaatacggc
    46981 ggagccactt actgggatac agaagcgtat gctgtccctc tttatcttgc tttagccaaa
    47041 cctgaagtaa ctaagaatct gctgaaatat cggcataatc aactgccgca agcaatccac
    47101 aacgctcagc aacaaggatt aaaaggcgcg ctttatccta tggtcacctt cactggtgtc
    47161 gaatgccata acgagtggga aatcacattc gaggagattc accgaaatgg cgcgattgcc
    47221 tatgcaatct acaattatac gaattacacc ggcgatacgg aatatttgaa aaatgaagga
    47281 ctggaagttt tagcagaaat cgcacgcttc tgggcagacc gtgtccattt ttcaaaacga
    47341 gcaaataaat acatgatcca cggtgtgacc ggcccaaacg aatatgagaa caatatcaat
    47401 aacaattggt atacaaatac gatcgcagct tgggtcttgc gttacacgcg agaaaattat
    47461 ttaacttatc aaaaagaagc tgctatcact atcagcgaag aagaactagc caaatggaaa
    47521 gatatcgcag agaacatgta cttcccagtt gacgaagaat taggaatctt tgtccagcat
    47581 gacacattct tagataaaga tctcatgcct gtatccgata ttcccacaag cgatcttcct
    47641 ctgaatcagc attggtcttg ggacaaaatc cttagatctt gcttcatcaa gcaggcagat
    47701 gtactacagg ggatttattt cttcaatgat acttttacaa aagaagaaaa gcaaaaaaac
    47761 tttgatttct atgaaccaat gactgtccat gagtcttccc tttcgccaag tatccattcg
    47821 attttagcag cagaattagg aatggaaagc aaagctgtag aaatgtacca gcgaacagct
    47881 cgcttggatt tggataatta caataacgac acagaagacg gcttgcacat cacttccatg
    47941 actggtagct ggttgtcgat tgtccaagga tttgctcaaa tgaaaactgc ccatgatcag
    48001 ttgagattcg caccatttct gccaaaaact tggaaagctt attctttcca tatcaattat
    48061 cgcggacgtt tattgttcat ctcagtcaca gatgaacaag ttactttcga gttattagaa
    48121 ggttctcctc tagaaattta cgtctatgaa cagtcttatt ctttagcgga tcgtctagaa
    48181 ataacgctaa gaaaggagga agcaaatgtt taaaggggta ttgtttgatt tagacggtgt
    48241 cattaccgat actgctgaat tccattatcg tgcttggcga aagcttggtc aggaaatcgg
    48301 cattacgatc gaccacacat tcaacgaaca gctaaaaggc gtcagccgag aagactcact
    48361 ctctttgctt ttagcttatg gcggaaaaga acactctttt tcaaaggaag aatttgcaga
    48421 attagcgaaa agaaaaaacg attattattt agagatgatc cagacgatcg aaccaaaaga
    48481 tgtcttcccc ggggtcgtgc ctttactgga ttctttaaaa gaagaaaaaa tcaagattgc
    48541 tcttgcttct gcaagtaaaa acgggccatt cctacttgaa aaaatgggtc ttacacctta
    48601 ctttgatgct attgccgacc cggcagaagc aacaaacgga aaacctgcgc cagatatctt
    48661 cctccttgca gcaaaagctg tcggactaaa agcagaagaa tgtatcggga tcgaagacgc
    48721 gcaagccggt atccaagcca tcctctcaag tggcgctcaa ccaattggcg tgggacgtgc
    48781 tgaagacctt ggagaggaga tcccgcttgt ccctgatacg acttttctta cgatcgatta
    48841 cttgaaagaa aagtggcatg accatggata atccagagat ctccgtcgta ccagttccag
    48901 agactgaaat aaagaaaatc acattgaaga acgactactt agaagtcgtt cttctcaatt
    48961 acggtgcccg cctgcaccaa atcttcgcac cagacaaaga aggaaaatca gaaaatgttt
    49021 tattgtctta cgatcgtttc gaagatgttc taacagacaa aagctttttt ggagcaactg
    49081 tcggacctgt agccggaagg atccgggatg cttcttgggg tgagcataga ttggagaaaa
    49141 actgcggatc acaccatatc cacggtggca cgaaaggctg gtctttccaa ttttgggaag
    49201 tagaagtttt caaaactcca tcatcaatag gtgtggtctt ttacttaaaa gatgaatttt
    49261 ctacctatcc aggaccaatc actgctacga tcacttaccg tttaacaaaa aacgaattag
    49321 agatgacaac ctctgctagt agccttgttg aaaccatctg caatccgacc aaccatgcct
    49381 acttcaacct ttcaggaaat ggaacaagag acatctatga acatcagtta acggtctttt
    49441 tagatggtgt gcttgaatta gatcaagaaa aactgcctac tggaaattgg acaaaaaaag
    49501 aaaaactgcc aatcgacttt agaaaatccc caacactgca agagatcctt gcatgttatc
    49561 cagatggact agatgatgta tttcttttgc atcatcctag gctttccaaa acttccttac
    49621 aattattcga gaagcattct ggtcgccaaa tgactatagc aacttctaat aaaagtatgg
    49681 tattattttc tacgactgga ttcgaagctg atttctctgt caatggaaaa cagatgcatt
    49741 caaactacgg attagcaatc gaaccgcaag aaatccctga tatcgttcat ttccccaaat
    49801 ttggatcgat caacctccat cctggtcaag aacggatcag tcagacgatt tatcggttct
    49861 ctgcacaata acaagagaaa ggtgttttta tcatggctat taccgtaaaa gatgtggcga
    49921 aaaaagccgg tgttgccacc tcgaccgtct ctcgtgtcat caacgatcat cctagtattt
    49981 ccgagacaac aaagaagaaa gtccgaaaaa ttatggatga actaggttat gttcctaaca
    50041 tgactgcccg caatctagga aaacgcattt ccagtgccat cggcgtaatc ttaccccctc
    50101 ttgattcaaa agagcggatc ggtaacccat tttatcttga gatcatggaa gcaatcaacg
    50161 aagaagcgcg gaattatgat atgactacag caatcgctac tgctaaaaat tttgaattgc
    50221 tgttagagaa cgtccagcgt atgcatttac aaaaacaagt agatggtttt attctagtgt
    50281 attcagatag tcaagatact gtcattgatt atttatacca aaaccacatc cctttcactt
    50341 tgatcggaca gccttatcat catgagaacg aaatcgttta tgtagataat gataaccagc
    50401 ttctaggtaa acaagcaaca gaatttttga tccagaatgg acataagaat atcctgtttg
    50461 tgacaaacac gactcatgaa agtttgtatt tcgaacgata tttcggttat caaaaagcaa
    50521 tgatgatggc tggtcttcct gcgttatcca gtgtagtact agagcatccg gaggattatc
    50581 tgtcctttga gaaaacgttg aaagaaaacg aagccacagc attggtggtc attgacgata
    50641 tcttcgccct tcgcacgatg cagttggcgc aaatgtatgg ctatgtcatt ccagagacac
    50701 tctccgtcat tagcttcaac aactcgatct tctcaacatt aacacaccct taccttacaa
    50761 gtatcgatat cgatatctcc gaattaggac gtgtcgctat gcaaaagctg cgcgaattga
    50821 tccatgaaga attgagcagt ggcgtacgtt tggtcatccc tcatcgcttg atcaaaagag
    50881 agacagtcgt tcctttagaa aacccatact aaatagctga ctcactaaat acgctgacca
    50941 tttatcctat ggtcagcttt ttatgtaaaa gaagatacta cttctaacac attgtttatc
    51001 tgtaaaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta
    51061 taaacggcgg aagcagaagc aactccttcg gaaataagct gaaattcaca aaaatttaaa
    51121 aagcaatttt cttgagtttc ttcttatttc ttggagttaa acgcttctgt cccagcctct
    51181 tttttccttc cttcaatttt ttacacgtcg acgattcaat aaaaaaccgc ctaatattgt
    51241 aaagacaggt gcaagaatcg agaaaaaggc gaatggcaag taatgcaacg cgctaacttt
    51301 catcgttccc ataatgaatg tgccgctgac tccccacggt acgagagaat tgacggtcgc
    51361 acctgaatcc gctagtgttc gtgtcaaata ggtttttgat aagccaacgt catcaaaaga
    51421 acgcttgaat gtttctcctg gaagaatgat ggacagatac tgttctccaa cgatcagatt
    51481 gattccgata cagctcaagg cagtaaaccc gactaaacga gaaggtcggt ccatcttttc
    51541 ttttaattct tgaacgatgg tatcaacgat cagatatttg atcagtaacc cacctaatgc
    51601 caatgcaagt aaaatcaaac tagcagaacc taacatactc atgatcccgc ctcgagaaag
    51661 cagttcatct agctttggat caggtgtatt tgcgacataa ccattcatca agatgtctgc
    51721 tgcttttccg accgtcaaac ttttctcatg gataaaagaa agaacgagag cagctgtcga
    51781 tcccgctagt aatgtaggga tagccggaat ttttttgatt gcccccccaa ataaaataac
    51841 tagtggaatc aatgtccaag gagagatcca aaagctcgct ttcatgtttt ggatcatttc
    51901 attgatacta gctagatcgg ctgaatgact gttatgtcct aatatcgtat aaaaaatcaa
    51961 cgaaaggacg aatgctggga aatccgtata acgcatacca gcgatatgtt cgaatagatt
    52021 aactcctcca atacccgctg ccaagttggt cgtatctgat aaaggcgagc agttattccc
    52081 taaaaaggca ccggaaacga ctgcgcctgt agtcattgca tcattgaatc caagtaaatg
    52141 accgatcccc aaaaaagcaa ttccaatcgt cgaaatcgtt gtaaatgaac tgccgacagt
    52201 cactccgaca attgcacaaa caagaaatgc tgtcggtaaa aagaaacgca cagaaacaag
    52261 tttaaatcca taaaccataa tcgtagggat ggttccagat aaaatccatg aagcaactaa
    52321 gacaccaatc aaaagaaaaa tcaggattgg aatgatccct ggagtgattc ctttgatgat
    52381 cccgtcatgg atctcatccc atgtgaactt tttaaatctg ccataaaaca acaaacacat
    52441 gaaagctgca aggatcggga tatgtggcga gagctggaat ccgatgatca gtactcctag
    52501 gagtgccaac aacacgatca atacagcgat actttctttt aaagaaatat ttttattcat
    52561 cgtcgatctc ctatttcaaa tgaaaaggcc ctgccatgaa aaaactattc acttggcagg
    52621 acctcctact aaatcagcga caaaacgtcg gctgaaagac ttattttttt atttttcttc
    52681 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52741 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52801 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52861 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52921 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    52981 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53041 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53101 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53161 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53221 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53281 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53341 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53401 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53461 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53521 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53581 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53641 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53701 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53761 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53821 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53881 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    53941 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54001 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54061 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54121 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54181 gggattcttt gagtttattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54241 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54301 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54361 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54421 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54481 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54541 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54601 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54661 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54721 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54781 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54841 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54901 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    54961 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55021 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55081 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55141 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55201 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55261 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55321 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55381 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55441 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55501 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55561 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55621 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55681 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55741 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55801 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55861 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55921 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    55981 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56041 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56101 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56161 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56221 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56281 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56341 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56401 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56461 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56521 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56581 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56641 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56701 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56761 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56821 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56881 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    56941 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57001 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57061 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57121 cttctgcttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57181 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57241 cgatagattg caggatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57301 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57361 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57421 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57481 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57541 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57601 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57661 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57721 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57781 tttatgaaag caggaacctg ctagcatatg taattaatta tttcttcagg ataggatagg
    57841 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57901 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    57961 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58021 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58081 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58141 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58201 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58261 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58321 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58381 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58441 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58501 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58561 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58621 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58681 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58741 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58801 tgattttgtt ttagttaatt tgaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58861 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58921 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    58981 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59041 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59101 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59161 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59221 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59281 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59341 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59401 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59461 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59521 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59581 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59641 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59701 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59761 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59821 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59881 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    59941 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60001 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60061 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60121 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60181 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60241 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60301 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60361 tctgaggttt attataacta
//
LOCUS       DAYYCW010000004        59758 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000004 DAYYCW010000000
VERSION     DAYYCW010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.004"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACHAYA_000224"
     CDS             complement(132..509)
                     /locus_tag="ACHAYA_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604061.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACHAYA_000225"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACHAYA_000225"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM8604062.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACHAYA_000226"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACHAYA_000226"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM8604063.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACHAYA_000227"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACHAYA_000227"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM8604064.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACHAYA_000228"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACHAYA_000228"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM8604065.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACHAYA_000229"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACHAYA_000229"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM8604066.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACHAYA_000230"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACHAYA_000230"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM8604067.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACHAYA_000231"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACHAYA_000231"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM8604068.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACHAYA_000232"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACHAYA_000232"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM8604069.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACHAYA_000233"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACHAYA_000233"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM8604070.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACHAYA_000234"
     CDS             10661..11575
                     /locus_tag="ACHAYA_000234"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM8604071.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACHAYA_000235"
     CDS             complement(11708..13273)
                     /locus_tag="ACHAYA_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8604072.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACHAYA_000236"
     CDS             complement(13266..14717)
                     /locus_tag="ACHAYA_000236"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8604073.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACHAYA_000237"
     CDS             complement(14731..15747)
                     /locus_tag="ACHAYA_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8604074.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACHAYA_000238"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACHAYA_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8604075.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHAYA_000239"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHAYA_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8604076.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHAYA_000240"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHAYA_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM8604077.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACHAYA_000241"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACHAYA_000241"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM8604078.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="ACHAYA_000242"
     CDS             21340..22764
                     /locus_tag="ACHAYA_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8604079.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22806..23063)
                     /locus_tag="ACHAYA_000243"
     CDS             complement(22806..23063)
                     /locus_tag="ACHAYA_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604080.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="ACHAYA_000244"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="ACHAYA_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM8604081.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="ACHAYA_000245"
     CDS             24337..25314
                     /locus_tag="ACHAYA_000245"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM8604082.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25339..26982
                     /locus_tag="ACHAYA_000246"
     CDS             25339..26982
                     /locus_tag="ACHAYA_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFM8604083.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26990..28396
                     /gene="lpdA"
                     /locus_tag="ACHAYA_000247"
     CDS             26990..28396
                     /gene="lpdA"
                     /locus_tag="ACHAYA_000247"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM8604084.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28558..28833
                     /locus_tag="ACHAYA_000248"
     CDS             28558..28833
                     /locus_tag="ACHAYA_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM8604085.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28839..29606
                     /locus_tag="ACHAYA_000249"
     CDS             28839..29606
                     /locus_tag="ACHAYA_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM8604086.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29774..31603
                     /gene="typA"
                     /locus_tag="ACHAYA_000250"
     CDS             29774..31603
                     /gene="typA"
                     /locus_tag="ACHAYA_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM8604087.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31864..33636
                     /gene="recQ"
                     /locus_tag="ACHAYA_000251"
     CDS             31864..33636
                     /gene="recQ"
                     /locus_tag="ACHAYA_000251"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM8604088.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33750..34052
                     /locus_tag="ACHAYA_000252"
     CDS             33750..34052
                     /locus_tag="ACHAYA_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM8604089.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34055..35218
                     /locus_tag="ACHAYA_000253"
     CDS             34055..35218
                     /locus_tag="ACHAYA_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8604090.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35244..38672
                     /locus_tag="ACHAYA_000254"
     CDS             35244..38672
                     /locus_tag="ACHAYA_000254"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM8604091.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38693..39817
                     /locus_tag="ACHAYA_000255"
     CDS             38693..39817
                     /locus_tag="ACHAYA_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM8604092.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39798..40244
                     /locus_tag="ACHAYA_000256"
     CDS             39798..40244
                     /locus_tag="ACHAYA_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8604093.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40228..40566
                     /locus_tag="ACHAYA_000257"
     CDS             40228..40566
                     /locus_tag="ACHAYA_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM8604094.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40685..41245
                     /gene="rsmD"
                     /locus_tag="ACHAYA_000258"
     CDS             40685..41245
                     /gene="rsmD"
                     /locus_tag="ACHAYA_000258"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM8604095.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41242..41733
                     /gene="coaD"
                     /locus_tag="ACHAYA_000259"
     CDS             41242..41733
                     /gene="coaD"
                     /locus_tag="ACHAYA_000259"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM8604096.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41723..42766
                     /locus_tag="ACHAYA_000260"
     CDS             41723..42766
                     /locus_tag="ACHAYA_000260"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM8604097.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42925..43653
                     /locus_tag="ACHAYA_000261"
     CDS             42925..43653
                     /locus_tag="ACHAYA_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM8604098.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43769..44014
                     /locus_tag="ACHAYA_000262"
     CDS             43769..44014
                     /locus_tag="ACHAYA_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8604099.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44011..44229
                     /locus_tag="ACHAYA_000263"
     CDS             44011..44229
                     /locus_tag="ACHAYA_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM8604100.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44365..44904
                     /locus_tag="ACHAYA_000264"
     CDS             44365..44904
                     /locus_tag="ACHAYA_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM8604101.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44897..47170
                     /locus_tag="ACHAYA_000265"
     CDS             44897..47170
                     /locus_tag="ACHAYA_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM8604102.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47208..48254
                     /gene="holA"
                     /locus_tag="ACHAYA_000266"
     CDS             47208..48254
                     /gene="holA"
                     /locus_tag="ACHAYA_000266"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM8604103.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48343..48597)
                     /gene="rpsT"
                     /locus_tag="ACHAYA_000267"
     CDS             complement(48343..48597)
                     /gene="rpsT"
                     /locus_tag="ACHAYA_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM8604104.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48997..49266)
                     /locus_tag="ACHAYA_000268"
     CDS             complement(48997..49266)
                     /locus_tag="ACHAYA_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604105.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49528..49656)
                     /locus_tag="ACHAYA_000269"
     CDS             complement(49528..49656)
                     /locus_tag="ACHAYA_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604106.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(49736..50191)
                     /locus_tag="ACHAYA_000270"
     CDS             complement(49736..50191)
                     /locus_tag="ACHAYA_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM8604107.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50329..52257)
                     /locus_tag="ACHAYA_000271"
     CDS             complement(50329..52257)
                     /locus_tag="ACHAYA_000271"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8604108.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52454..53287)
                     /locus_tag="ACHAYA_000272"
     CDS             complement(52454..53287)
                     /locus_tag="ACHAYA_000272"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM8604109.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53479..54183)
                     /locus_tag="ACHAYA_000273"
     CDS             complement(53479..54183)
                     /locus_tag="ACHAYA_000273"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8604110.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54206..54832)
                     /locus_tag="ACHAYA_000274"
     CDS             complement(54206..54832)
                     /locus_tag="ACHAYA_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8604111.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55158..57533
                     /locus_tag="ACHAYA_000275"
     CDS             55158..57533
                     /locus_tag="ACHAYA_000275"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM8604112.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57557..58048)
                     /locus_tag="ACHAYA_000276"
     CDS             complement(57557..58048)
                     /locus_tag="ACHAYA_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM8604113.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58235..59155
                     /locus_tag="ACHAYA_000277"
     CDS             58235..59155
                     /locus_tag="ACHAYA_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8604114.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59309..59653
                     /locus_tag="ACHAYA_000278"
     CDS             59309..59653
                     /locus_tag="ACHAYA_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM8604115.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT        19620 a        10332 c        12851 g        16955 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc
    25381 gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat
    25441 acattactag aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga
    25501 acggttaaaa atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta
    25561 gaaatcgatg caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc
    25621 caaactccag ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca
    25681 agcgaaggag tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa
    25741 atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa
    25801 gttcaaaatg acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac
    25861 gtgattgttc cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct
    25921 cctggacaca atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa
    25981 gtggaaacat caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg
    26041 gctatgccat ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact
    26101 gcaactggaa aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt
    26161 ccaagcagtg caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct
    26221 cctgcagcag aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac
    26281 ttggaagaac gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac
    26341 agtaagcata cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg
    26401 gataatcgga aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg
    26461 ccatatgtcg tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct
    26521 atcgacgatg caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact
    26581 gatacggatc acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt
    26641 gctattgcag acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca
    26701 gaagatatgc gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg
    26761 tttacaccag tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa
    26821 caaccaatcg taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta
    26881 agtttcgatc accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa
    26941 cgtcttttag ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg
    27001 agattttgct gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc
    27061 tgccatccgc gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg
    27121 cggtgtttgt ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca
    27181 ttatcaagag tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga
    27241 ttttgcaaaa acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt
    27301 tggtttccta ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga
    27361 tgatcacaca ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc
    27421 aatcatcgca acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt
    27481 gttagattct actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg
    27541 tggcggtgtg attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac
    27601 gattcttgaa ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt
    27661 agaagatgat ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc
    27721 agtcgataac ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt
    27781 tactgctgat tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt
    27841 agaacaagcg ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg
    27901 tacgaacgta cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca
    27961 caaagccagt tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt
    28021 agattataaa gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat
    28081 gacgatcaaa gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc
    28141 agggaacggt cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac
    28201 gatcgaagat aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt
    28261 ctctgaatta gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat
    28321 tcatcctcat ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt
    28381 accaatccat atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt
    28441 ttgtttttta gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg
    28501 gtttattacc acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg
    28561 caagaatatc agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg
    28621 aagatgtggg aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg
    28681 aagacttatc agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga
    28741 cgggaatttg aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa
    28801 aatccaaaca aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata
    28861 tcaagtcctg gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa
    28921 tctcgcaaaa atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt
    28981 ttctaatcga aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag
    29041 ggtgtaatca attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca
    29101 ccttgaattt tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg
    29161 gcgttggaaa gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa
    29221 aaggaatagg cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat
    29281 cggaaggctt acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag
    29341 aaattgcgga aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg
    29401 caatcttgaa aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag
    29461 cgggaaatgt cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt
    29521 tgacatttga aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg
    29581 cagacctttt gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa
    29641 aacgagcaaa agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa
    29701 aagtctgcta taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa
    29761 ggagttagtt acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt
    29821 tgaccacgga aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc
    29881 acatacgcaa ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat
    29941 cacgatttta gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga
    30001 tactccagga cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg
    30061 cgttgtgttg gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa
    30121 aaaagcactg gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc
    30181 tgctcgtcct gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga
    30241 tgatgatcag ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt
    30301 gtcagatgat ccagcagatc aagaaccaac catggcacct atttttgata caattattga
    30361 aaaaatccct gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact
    30421 tgactacaac gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa
    30481 agtaggagac caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac
    30541 gaaattattt ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga
    30601 cttgattgct gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga
    30661 tcatcaagat gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt
    30721 agttaataat tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga
    30781 agaacgtttg atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc
    30841 tccagatgca tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa
    30901 catgcgccgt gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat
    30961 cgacggtgta aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat
    31021 gggtagtgtt atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac
    31081 aggaaacgga caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc
    31141 aactgaattc ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata
    31201 tctgccaatg cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga
    31261 tacaggaaaa gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt
    31321 agaaccaggt acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga
    31381 tttgactgtc aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga
    31441 ccaaacatct gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt
    31501 gaatgatgat gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt
    31561 agagaaaaat gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag
    31621 agagaaacca gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt
    31681 ggagaagtca tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa
    31741 gacttttaaa aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga
    31801 aaggagatca acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg
    31861 aagatgaacg acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa
    31921 gaaattatcc agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt
    31981 gggaaatcaa tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg
    32041 tcccctttaa tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc
    32101 gccactttta tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc
    32161 gatagacagc agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt
    32221 atccaagcaa tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc
    32281 tctcagtggg ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa
    32341 ctgccaaatc gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct
    32401 gacataaaaa gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga
    32461 gaaaatctac gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat
    32521 ttaaagatca acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac
    32581 cgtctctatc atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta
    32641 aatgagaatg agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt
    32701 gtagcgacaa atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat
    32761 tatcaaattc ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat
    32821 ggtctttcca gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt
    32881 tttatccagc agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa
    32941 gcgatgacag aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt
    33001 ggcgagacaa gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta
    33061 gaagtaacga cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat
    33121 ggtaaacaaa tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta
    33181 ggttttggac atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg
    33241 cagttaattg aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct
    33301 gttttgaaag taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga
    33361 aaggaaccca agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg
    33421 ctgcgggaac tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt
    33481 tcagattcca cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt
    33541 cagataaaag gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg
    33601 ataaaaaatg cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa
    33661 taaaaacaac tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat
    33721 taaggaatta gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc
    33781 aagtattaaa tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc
    33841 tctgtatctc acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt
    33901 cccggcaagt cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga
    33961 tgttaagtga gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt
    34021 atgataaaaa agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca
    34081 tctattagac tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat
    34141 ggtatatagt tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt
    34201 cattaaccag agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat
    34261 gaaaacagat gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat
    34321 tttgctgctg attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca
    34381 aattgcagga ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct
    34441 gtctctgact ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa
    34501 acgtccactt gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg
    34561 taatgcggca gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta
    34621 tgtgtatact ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct
    34681 gatcaatatc acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt
    34741 tgctatttac caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg
    34801 ctactatgcc atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa
    34861 aaaaggattt ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact
    34921 gggagtgatt atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt
    34981 ccttgtaggt atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc
    35041 aatgtttttg atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac
    35101 agggatcacg ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg
    35161 tgtcggcttt gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata
    35221 aaaaaagatg taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg
    35281 ctgtacgtgt atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag
    35341 aagaagatga atattccgta caccgtttca aagcggatga agcatattta gtaggacaag
    35401 gaaaaaaacc gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat
    35461 gcggagctga cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc
    35521 aacggtgcga agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt
    35581 ttggagataa aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag
    35641 gcacagatgg accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt
    35701 atccgattat gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg
    35761 atgaaaagag tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg
    35821 gtagtgatga agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga
    35881 ttctcgggga tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc
    35941 gccgtcacca aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc
    36001 aaaaaatctg ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta
    36061 cagtagaatt tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc
    36121 aagtggaaca tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt
    36181 tgattgctca aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta
    36241 aattaaatgg ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt
    36301 taccagacac agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg
    36361 atgtcggaaa tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa
    36421 aagtatgtac gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga
    36481 aagaatttag aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac
    36541 atcctgaatt ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat
    36601 ttgaatttcc tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta
    36661 ctgtgaatgg ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta
    36721 tgcctaaaga tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga
    36781 agggtgccga tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata
    36841 cgacatttag agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca
    36901 agcagattgc acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg
    36961 ggggagcaac atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga
    37021 gaaaaattcg ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg
    37081 ccgttgggta ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag
    37141 cgcaaggaat cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa
    37201 aaagtatcca agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg
    37261 gcgatatcaa cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa
    37321 aggaactaga acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta
    37381 aaccgcaggc agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc
    37441 atctgcatac tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag
    37501 caggagtaga catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta
    37561 gcatgagcag cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg
    37621 aaaatgcaca gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg
    37681 aaaacggctt gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt
    37741 attcgaatct acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca
    37801 aacaaatgta tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat
    37861 ctaaagtagt tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata
    37921 tttatgaaaa aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag
    37981 aactagggca acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca
    38041 gacctgcatt gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa
    38101 aaaaagaatt ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt
    38161 tgatgtatcc gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta
    38221 cattactgga cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac
    38281 aaatcgaacg cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg
    38341 aagggaatcg cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag
    38401 ataattcgat catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac
    38461 aaattggagc aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg
    38521 ttaaaaaagg tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag
    38581 cacgttttga cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg
    38641 gtgacctttt gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag
    38701 actgggtttg ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt
    38761 cttgtttccg ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat
    38821 gacaacgtgg aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc
    38881 gatcgatcag ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg
    38941 ttttgaaaca agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca
    39001 agacagcaga gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt
    39061 ttatttaggg atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac
    39121 ttttgagtac catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg
    39181 tccgctaatt gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa
    39241 cggcctattt tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca
    39301 agtgtttggt gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat
    39361 caatcgcgtt aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga
    39421 acttcccata tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga
    39481 ttttttgaca aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc
    39541 gctaggcagt gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt
    39601 aatcagtaaa caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc
    39661 tactttttcg tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc
    39721 gggaacggat tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga
    39781 agaaaacgga gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg
    39841 atcaatgcct ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg
    39901 aacataaaca gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt
    39961 ctaagaaaga agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg
    40021 aaaaaaaacg agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag
    40081 ctttccgata tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg
    40141 ccgaagaagt ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa
    40201 aacactttgg atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca
    40261 aaacgacgcg ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag
    40321 ttcggcttgt tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa
    40381 gagacattgg aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa
    40441 cgttcttacc gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta
    40501 ttaaaagacg aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat
    40561 ctttaaaaat ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt
    40621 ttgatcattg tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga
    40681 gtagatgcgg ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc
    40741 gaacaccaga ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata
    40801 ttttgatgga gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc
    40861 agtatctaga ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat
    40921 catcaaagaa aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga
    40981 tgcggacaaa gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct
    41041 tgatccgcca tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg
    41101 acttttttct aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga
    41161 aactgtggct ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat
    41221 ctatagaaaa gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt
    41281 acaaaaggtc acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt
    41341 ggtgttttta tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg
    41401 atcacaaaag ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg
    41461 acggtagaaa cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc
    41521 aaagactttg aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg
    41581 gaaactgtct tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa
    41641 gaagtactgc actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct
    41701 ttagctggaa agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat
    41761 catatttgga atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc
    41821 accgggagcc acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg
    41881 ttctgggtct ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt
    41941 aaaagctaaa atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc
    42001 gacaaatgaa gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc
    42061 aatcgaacaa gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata
    42121 tgtcatgagt gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt
    42181 aacaaaagta gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag
    42241 ccaaaaagtt ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc
    42301 ttctggtgat ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac
    42361 tgaccatacc gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg
    42421 gccatcagcg ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt
    42481 gcgacatggt aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg
    42541 aatcggagga atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt
    42601 tgcaccagat gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa
    42661 ctacgaagaa gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt
    42721 aaaaacagta caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa
    42781 agaggggttg tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat
    42841 aaaaataaaa acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag
    42901 agctcactgt ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc
    42961 tttgaaaaaa gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg
    43021 atcatttgga tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca
    43081 aatttgctat tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt
    43141 agttccagtg gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca
    43201 aaggtcatgt acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct
    43261 tcagaagaac gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat
    43321 agcgatcgaa tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct
    43381 gtaggtgaag agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct
    43441 acagcagatt ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag
    43501 cagattcctg ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac
    43561 ggatccttcc agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt
    43621 gaaaaactaa aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc
    43681 aataaatgga cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag
    43741 gaggtagtat ttttggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc
    43801 aatgctgaat gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt
    43861 cagcggcaat aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac
    43921 agcagataaa gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc
    43981 taaagaaacg taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt
    44041 tctgatcatg ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg
    44101 aataacaagt tgatcaaaga atatatctct gttctcacag aagtagcaaa acgccatgat
    44161 tgtgaagtat cgatttttat acggaatttc ttgaacaaat ggattcgaag aatttttata
    44221 atgggctaat ggatgacgga cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca
    44281 ggatgattcg ttatatttaa agaatatttt gtttaagcaa tgagtacttt ttaatcaact
    44341 tgaaaaaacg gaggaatata aaacatgtta gaaaaatctt caaaagcatg cgatacgaat
    44401 aaagaaaaag ttactgataa gtcacataat gaacgcattc cttgggacca gtactttatg
    44461 gctcaggcag tactgttatc tttacgtagt acgtgtacac gtttagaagt aggagcgaca
    44521 ctcgttcgag aaaaaaggat cattgctggc ggctataatg gagcagtcag tggagatgtc
    44581 cactgcatag atgaagggtg ttacgtcgtg gatggacact gcttgcgaac gatccatgca
    44641 gagatgaatg cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc
    44701 tatgtcacac attttccatg tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa
    44761 gggatcaatt atctccatga ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa
    44821 ttaggtgtat ctgtacacca agttgcttta gactcacagc atttttcaaa acttcaaaat
    44881 gaattaacag gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt
    44941 tccggcaatt acgtcgatga ttatctgttt gttgtattac actgacaatt ggggttatcg
    45001 tttgcttctt gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta
    45061 cttgtgtatt ttgtgtagca taacggtatc catttcttat aggctacaag agtattcatt
    45121 tttagaggaa aaaacaacat ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa
    45181 tggtaatttt ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc tccagctttc
    45241 ttatcaagta aacacagaag cagaattgaa aacatggctt aacttccgag gaaagggaat
    45301 agaattgact gctgaaggcg agttcatctc tccagcaaaa aagcggaatc tttataattt
    45361 tgaccaagaa gcttacttca ggaaccatag aatttctgga cggttccaaa ttcaaaagat
    45421 tgaacagatc accatgcaaa atcactggcg aaactggctt cagagaaaaa gaggatcttt
    45481 tatctccttt gtccaatctg aatttccgca aaaaacaagt gtatatatta attctttatt
    45541 atggggctat aaagatgcag gattcagaga accagaagaa atttttaggg agagtggttt
    45601 gctgcatctg ttcagtctat cgggtctaca catccaattt tatttagggt ggctgtatta
    45661 tttgtttcga cgtatcggat ggccactaca cgtcagtatt attccgttga gtctactaac
    45721 tatctgttat gtttctattg caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt
    45781 tttattgcga ttgctagtca aattgctgaa taagcattat tccaccatgg acttatttgc
    45841 agttgtcttt tggcttcttt tactatttga tcctcgattt ctttttcatg cgggtggaca
    45901 gcttagtttt ttcatgagtt ttttgtttat cctgatttct tttgatggag attggttgaa
    45961 aaaaacggcc tctcacgtcc tgtttactat gatcacgatg ccgttgattg tatggcattt
    46021 ttatgagtgg cctattttag gtagtctttt tacattactg attgcaccgg tattcaagtt
    46081 tctttttctt cctggtgtgt tatttctcac actcttcaat cgctggctgc cggaaacttt
    46141 acttttgctg atagaagagg gattaatctt atttgagcga ataatcgatt tttcttcagg
    46201 gtcgtctctc attataggac gtttgcccct tgtgctatct gggatgtata ttttcggatt
    46261 actgatttgt atggataggt tgaaagaaca acctgtgaaa cacttgtttt atgttggtct
    46321 tttttctttt atttttttga ctttgccatt tttacgcttt tcttcaacga tcgcttttat
    46381 cgatgtcggt caaggagaca gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt
    46441 gatcgatact ggaggtcagt tacactttga aagagaaaag tgggcgcaag gtatgatacg
    46501 tcctcctgct gaaagaaata tcgttccata tctaaaaggt caggggataa gtgtaatcga
    46561 taaactcatc ttgacacacg acgatacgga tcatgtggga gaattaccca cgttatctca
    46621 gcattttact atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg agccattaca
    46681 aaggcattta tctgaagctc aaaaaaatgg tactgagatc attgaaatca aacatgggga
    46741 caggatcagc ggttattatg atctttatgt attgactcct tttgaaaagg gagagggaag
    46801 aaatgaagat tcgattggtc tatggatgga atataacaat aggcgctttt tatttttagg
    46861 agatttgaat caagcaatgg aaaaacaatt gcttttgatt tatcctaatc tgaaagccga
    46921 tgtagtcaaa ttaggtcatc acggtagccg tacgtcaagt aacacagatt ttctttccca
    46981 tattgaagca aaacatggga ttatatcctg cggcgtaaat aatcgttatg gtcacccgca
    47041 cagcgaagta ttggagacac tagaagaaaa tcagataaaa acatggcgaa cggatcaaca
    47101 aggaatgatc acctatcgct ggcatccagt ttttcatcct catgggctag tggaaacaat
    47161 gatagactag taattctcat ctgctcatgg taggataatt tttgaaagtg gtgagtaatt
    47221 tgagtataca agcagagtta caaaaaatac gtaaagaaaa acttgcatct tgttacttga
    47281 ttttaggaac agaaaagttt ctccaagatc aagttcggac agaaattgtc aaaaaaatcc
    47341 aattagaggg cgaagatgac ttaaattttt taagttttga catggaaaat gcaagtttag
    47401 atgaggtagt agctgaagca gagacgttgc cgtttttcgg agatcaacgg ttagtattcg
    47461 tagagaatcc ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg
    47521 atttattgac ggcttatttg aaagaaccat tagaatcgac tgtattagta ttctttgcgc
    47581 catatgaaaa gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa acagcaatta
    47641 tcatcaatgt ccaacagttg aatgaaaaag aagtacgtca atatctgatg aataccttag
    47701 agaattcctc aatcaaaatg gaacgaagtg caattgattt attcttgagg ttgaccgact
    47761 tagatttatc caaaatgatg agagagttac agaaattggt cctttatgga caaaatcaac
    47821 agcaaatcac ggtaagagaa gtagaagaat tagttcctaa aacgttagaa cataatattt
    47881 ttgacatgac ccaatatatt ttaaaaggga agacggaaca agctttgcgt ttatacgaag
    47941 atttagttat gcaaggggaa gaaacaatca aaattaatgc gattttgctt tctcaattac
    48001 gtttattttt acagacaaaa tttcttgtaa agattggtta tcagcaggca aatattgctg
    48061 aaacattgaa aatccatcca tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg
    48121 atgaacattt gctggtacag ttgtttgatc aattagttga gatggattat caaatcaaaa
    48181 cgggacaaat cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca gggcagttgt
    48241 taagaaatcg ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt
    48301 cctttttttg acaaataaaa aaatcggccg aagccgattc ttttatttag cgattttttt
    48361 gcttaaacga gatttgtcgc ggttagcttt gtttttgtgg attagacctt ttgattcagc
    48421 catatcaata gctttaacag cttctttata taacgcatct acgttgtcag cacctgaagc
    48481 tacagcatct tcaaattttt tgattgcagt acgcatagcg cttgtttgag atgaattttt
    48541 cacattagcg ttttcgcttg tacgaacacg tttgattgca gattcaatgt ttggcattag
    48601 tttcacctcc gataacttaa gttcttacgc gtgttaccgc ataattcaac attcattatt
    48661 atacctaaag gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc ttaacagtct
    48721 tttattagga ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac
    48781 gctattgtgg gaggcaatag cgtcaaacca aactagattt ttatttatct atttaaacaa
    48841 agtttgaatg ggaatgtttg gtactaagtt attatataac aaagagaacc gtttgcaaag
    48901 ttttttttga aaagggatac gattccctgt tgttctttgt ttttctatta agaaactgca
    48961 gttattttat aaccagtatc ctttgatcag taaaggctat tctggtgtac cgattatttt
    49021 aatgtattca tcgatatttt ttatcaacat tttttttatg accaactggt tccgacggtt
    49081 ccatttgcca cttacttcca gcacatattt atttaggggc aaacgtaaga tatattttgc
    49141 caaatagcga tctttaagca ggccattgat cacacggtcc tcaagctgca agttgatccc
    49201 agtcagcatc attggctgtt cttgtaaaac gtacgtatca attaaaagac cttcaaaagt
    49261 ttccacaagc gtcttccttt cttttcaaag taacctgaaa acaggcacta attcttccat
    49321 tttgatattc gttgaagtgg atgcgatcat tctttctttt gtccactgta atctagtata
    49381 acaaaaaaga gcagaagagc caaaaagaaa aaagagcatc gaattctata ggaagtctca
    49441 ttggcacggc ctaaattatt acaaaacatt gattaaaaaa agcccctgca tcgcaggagc
    49501 atcccctata tcgttttaat aggtcaatca aaaagcagta tcaaacacat tttgtatcaa
    49561 atcaggaaat aatagataag ctaagcccag aatcaaaagt accgctagaa gaaaccagcc
    49621 gattgttcct atgaaatact ttaatttttc gaacattgta tcacctcgtt gtcagtatat
    49681 cacggatcag taaattgggg gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt
    49741 ttcctaaagc aaatacttgt aagatacagt aagtgatacc agaagcaatg accggtccgg
    49801 cggcgattcc tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga
    49861 tttgtggatc agaagcaatc aatcctacac ccttggaaga taagacagcc actagcccac
    49921 cgcaaaaaac agctacccag ccaatagggg atttaaaggc ttctaataga tcacgtagac
    49981 cgatttctcc tgttgcgata gggattagaa tagccgctgt gatgatcgtc accccaatat
    50041 tgattccttt gccttgaagc caagggaaaa aggaacggga ggctggtatg agcttcataa
    50101 tcaaaacaaa agctgccgct atcatcagac tttgattttt agctaaaatt gctacaagta
    50161 aaataattcc taaaaaaagc caactttcca tgaaatatat tcctcacttt cttcgaaaca
    50221 atcataacat gtttttagaa agttacaaaa gtaggaattc atccttcaag acctattcat
    50281 aagctgtttt tttcataaaa taaacaaaat cataaaaaaa cactttgctt atttgacaga
    50341 agacagccgc gctttgtata gtggacaaga cttacatttt tttgaagaat cagaaagatc
    50401 aggtaaaggt cttagtaaac gcaagccatt cagaataacc aatattgtgc ttccttcgtg
    50461 tcctacaact ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag
    50521 aataacaaga atcgaaaaaa taatattttg tttgatgatc cgccgcattt ttaaagatag
    50581 ccgatgactg tagacaaatt tatttaagtc gcttttcatt aagacgatgt ctgcgatatc
    50641 catagcggca tctgtccctt gccccatagc gacacctaaa gaagcggctg ctaaagcagg
    50701 cgcatcattg actccatcac caaccatcgc atttacttgg taaagttcat tctctttttt
    50761 gactagttgt gttttttctt ccggagtaca gcttgcatag taatgttcaa tccctacttg
    50821 ttcagcaatg gcctttgctg tgccagaatg gtcgccggtt atcatacttg tatggatgtt
    50881 ttgggattta aagtaggaaa ttacttgttg ggtatttgct tttggaatat ctagcagacc
    50941 taaaacagca attaactgct gatctttaga aagataaatc acggttttgc cttgattttc
    51001 caaacgttcg atcatttccg caatctcttt gctgattatc atcgtctcag gatcatatgc
    51061 gtgcttgcca actttccatt gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat
    51121 ttcttctatt ggtaagtatt ggatttcttc aggtattgtg caatcgaaat attgcaaaat
    51181 cgcatgagca agtggatgcg tcgttttttg ttccatagca acaagaagtt gttgtgccaa
    51241 atccttttca tcaaagaaaa agctgtcagt tacaactggt tttcctttag tgagcgttcc
    51301 tgttttatca aaggcaatcg cttttaattc agaaagcttt tctaaatgga taccgccttt
    51361 gattaaaatc ccattttttg cagaatttga aagagcagct agagaagctg gtgtagcaga
    51421 tgcgacaagc gcacaaggag aagccacaac taataatacc attcctcgat aaaagctttc
    51481 atcccaactc caacctagga aaaagtgggg gagaaggatc attagaggaa cagcaagcaa
    51541 aatcaatttt acataattat tttcgattcg ttcgataaat gaagctgttt gagttggcgt
    51601 attctgcgct tcttctacta attggataat cttagcaaag acagtttcgg aactcgtttt
    51661 tgttactcga atcgtgatgg gatttccttg attcagcgtt cctgcataca tctcatcgcc
    51721 taaatatttt tctacaggca cagattcacc gcttaatgca gactcatcga tcgttgcctg
    51781 tgtgctttct aaaaagccat ctatcggaac agctgctcct ttagcgacaa ataccaagtt
    51841 atccaccttt agttgagaaa catctacttc ttgcatttgt ccattttctt ttaataattg
    51901 ggctttttgt ggttgaagat tcatcaaagc agtaatttct ttttgacttt tattagttgt
    51961 gtattcctct aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta
    52021 attaccgatc aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt
    52081 ccgatcattc cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat
    52141 ggcactgata taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa
    52201 gatcataaat aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt
    52261 tattagacct ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac
    52321 agctaaatga gaacgataat ggttatcatt tggtgattaa gcttgatgtt gtttttatta
    52381 tttataatga ttattattaa agaaaataaa aaaccagaac atttctcaaa taagagaagt
    52441 gttctggttt tttctacatt gcaataaacc aataaccaat caaaatgata ccaaggataa
    52501 tacgatacca gccaaaagcg gtaaagtcgt ttttcttcaa atagcttagt aagaatttga
    52561 tagctaagac ggaaaccaca aaagatacga atgttcctac aagaagaaca acagtttctt
    52621 ccattttgaa tgaattccct tcagctaaat atttgacgat cttcaagccg cttgcgccaa
    52681 acatgacagg aatccctaag aaaaatgaaa actctgtagc gacaaaacgc gaagcaccaa
    52741 tcaagataga tcctaggatc gttgcgccag aacgggatgt tccgggaaca agtgccaaga
    52801 cttggaataa cccaacaaga agagctgctt ggtacgtaaa gtcattcaga gaagtccaac
    52861 gaggtgtttt gtttttgttg cgtttttcga caataataaa ggcaataccg taaacgatca
    52921 aaacaatggc aactggtaag aacttgtgga aatttgcttc taaccaatca tccaaaggca
    52981 aaccaatgat gatggctggt atgcaagcaa cgatcacttt aaaccaaagt gtccacgtat
    53041 cttttttttc taccgttgat ttacttggag aaaatgggtt taacttgtga aaataaagaa
    53101 cgacgacagc caagatcgcc cctaattgga taacgacatt gaacatcgac ataaattgtg
    53161 aactggcatc cagtttgatg aattcatctg ctaggatcaa atgaccagta ctactgatgg
    53221 gcagccattc cgttattcct tcaataatac cgagaatcac tgctttgata atatttgcaa
    53281 aaaacataaa ttccttcctt cccaattaag atacatctta tagtataccg ttattttttg
    53341 agaaacccaa tctgtttttt aatattaaga aaaacttgag aaataaagca gataagtaca
    53401 cgaagagaaa aaagaagaca aagccctcgt tcagactttg ccttttatga ttctctggta
    53461 aaaattaaaa tatccccatt atagaaacga gtcgcgacta tgtgttttcc cgttatcgat
    53521 ataagacgta tcattgatgc cgtggataga aaatcgaccg tttgcataag tgacctttgt
    53581 tacgctgcca tttaaaagcc cagcacgaac aggctgttta ggatcgatca gattaagcaa
    53641 aaaggcgatc gtcaaaccat gagatacgac taaaacattt ccaccaccat ttttttcgca
    53701 gtgatgggcg atatcttcaa atcctgtcca tacacggtct cgcaatgttt cataagtttc
    53761 cgcccaccca gcggtatcta agttgtatac agcattagca atttgttcaa aagaaacttc
    53821 tgtggtaaac atttcatcat atgttttaaa attcaataca cgagggatca caccccacat
    53881 ttccatgtca tatccacctt caagagaccc gaaacaccat tctctaatcc ttttttcaat
    53941 aaaataagga atttcttttc ccttagaatg ttctcctaga ataatacggg cagtttcgat
    54001 tgcacgacca ctgtcactag aataagcaaa tgaaaagtcg acatttttta acccgcgacc
    54061 taaataatga atgccttctt gtccttgctt tgtgaggggt gtatcacacc agccttgtac
    54121 tcgttcaatt gtattgaaca tcgtttttcc gtgtcgtacg atataaagtg tagtttctgt
    54181 cattttattc tcctcctaag tcgttttaac gattgaaaaa aggatttgtc cttttttcct
    54241 gttcaatcgt cgttgcatcg ccatgtcctg gatagactgc aaattcatca ggtaatacaa
    54301 acagctgggt ccgaatacta tgaagtaatt gttctaaatt ccctgtatac agatcggttc
    54361 taccgatact tcctctaaac agggcgtcgc cagaaacgac aaaatcatca aaaataaagc
    54421 tgacgcttcc tatagaatgc cctggggtag ggacgacgcg aaaagacatg tttcctaaag
    54481 tataagtttt catttcaaat tcatattctg caggcgagac aataacatca gcaatatcat
    54541 catgtctacc gagtccagaa agattcaaaa tcggatcacc tagccatttt tgctctagtg
    54601 ggctgacata aactgggatt tgataatacg cgcgtaaagg ttccactgca ccgatatgat
    54661 cgtagtgagt atgggtaagc aaaatcgcta ccggctgttg gtttgttttt ttgatttctt
    54721 gtttgattcg ttccgcatct tctccagggt caatgatcaa aagtgcttct tcgttgtaga
    54781 ccaaataaca gttttcttca atcattcctg tttttaatct ttcgatatgc atgtgcattc
    54841 ctccttgata gccaaaactt ctttcatcca ttagtataac gcattcattg ctaggaaaca
    54901 aaaacaggta cttatttctc tttacttatc tgaattatca gactatatga aaagtatttg
    54961 gatattctaa agagaaggaa agtaagtttt catagcattt taatatgtcc ttgttacatt
    55021 agattcatcc aaaaatccaa attctatatc ctatatccct gcctaatcag cagggtgttt
    55081 ttttatgttt ggaaagagag ctctttttgc taaactaggt tttttcaggt aatcttagag
    55141 agaaagaggt ggtggaaatg atcaaacagt caaaaatatc tgtgcggcac ttagtagaat
    55201 ttattttgcg acgtggaagt atcgacaatc gtaaaaaaag taaccatact gcattagaag
    55261 gagcgagaat ccaccgcaag ctccaaaaag atgcgggaga aaactatgaa aaagaagtct
    55321 ttttaaaaac aacagttcaa ttagatgaat attacctgat cgttgaaggc cgtgcagacg
    55381 gtattttcaa aagagacggt atttattata tagatgaaat caaaacctcc gaaccacgat
    55441 ttgaagattt agaaccagaa caagtggaac ttttctttca tcaagcaagg gtgtatgcct
    55501 atatctatag tcaagaacat gatttacaag aaataaatct gcaattgact tacttccaga
    55561 cggcagaaga agtgatcacg agaaaggttg aacatcagac aagagagcag ttagaaactt
    55621 tttttaaaaa gctgactgaa gattataaag aatggcttgt ttttcaagaa aactggcgca
    55681 ctgtacgcaa tgcctctttg atggctttga aattccccca tgatgagtat aggaaaggtc
    55741 agcgtgaact agcggtagct gcttataaaa cgatccgtac gaaacagaaa ttgtttgtag
    55801 aagcacctac cggaacagga aaaacaatct ctacgttatt ccccgctttg aaagctgtag
    55861 gggaagaaga gggagaaaag atcttttatc taacggctaa aacaataact agacaagtag
    55921 ctgaagatgc tatgacagca ttaaaagata caggagctga agtaaaaagt gtcactttga
    55981 cggcgaaaga caagatctgc tttttagatg agacgacctg taatccagat cagtgcccat
    56041 atgccaatgg ttattacaat cggattaatg aaggtttatg ggatcttttg aatcatgaga
    56101 atcagatcac tcgtgaagtc atcgaaactt atgcaagaaa gcatacactt tgtcctttcg
    56161 aactatcatt agatgtcagt atctggtgtg acgtaatcat tggggattac aattatttat
    56221 ttgatccaac tgtttattta agacgttttt ttgaagatga aaaaaacgag gactatctgt
    56281 ttttgattga cgaagcacat aatttggtga atcgttcccg agagatgtat tcggcagaac
    56341 tatcttatga aaaaacgaaa cgaagtaagg aagctatacc aaaggaattc aaaaaacttc
    56401 atcgccgttt caacaaacta ttaaaagaat ttgattcgat ccgagaaata gcaaaagaag
    56461 atcattggga ttatcatcat caaaaagcac cggctgaatc tttagtaaag gcaggttatc
    56521 aattaagcga aaaaattaaa gaatggctgg cagaatttcc tgagcaccca tcacaagaac
    56581 aattgcttcc ttattatttt gatttgcttc attttttgaa ggtcagtgaa ttttatgatg
    56641 atcactacga aacaacagtc gaaaaaacgt atcgcgatct gatagtaaaa gagttttgta
    56701 ttgatccaag cttgtttctt gagcagtcgt tggataaagg aaaaagcagt attctttttt
    56761 ctgctagttt ttcaccactc tcttattatc aggaaacact ggggggacaa aaaagtctgg
    56821 cttacaaact gccgagtcct ttcccagaag gaaaccagca agttttcatc gcaaattacc
    56881 tagagacgac ttatcgaaaa agggaacaca gtctggcaaa attagtagag acgattcatg
    56941 cttttgctga tggaaaggtc gggaattatt tcgttttctt tccttcttat caatatttag
    57001 atcttgcggt ggaagcattc agacagcaat atcccgacag caatgtgcta atccaagaaa
    57061 cggacatgaa tgaggaagaa cgcgaacgat ttttaacaaa atttcaaaca aatcccgctg
    57121 aaacattgat tggattttgc gtacttggcg ggattttttc agaaggaatc gatttgagag
    57181 gggaccgatt gatcggcagt atgatcgtag gtgtaggttt gccgcagatg aatcatgagc
    57241 aggaactgat caaatcatat tatgatgaga aggaacacct tggatttgct tacgcttatc
    57301 agcttcctgg aatgaacaaa gtcttgcaag ctggtggtcg agtgattcgt ggcatggcgg
    57361 atcaaggaat cgttgtattg gcagatcaac gattttctag tttttcttat cgacgtttat
    57421 tcccacaaca ttggcaaaca gcaagagaag tacgaagtgt tggggaattg gaagaaggaa
    57481 tacagcgttt ttggttatca aaagaagcag taaatcaaaa aaacgaggaa tgacagacgt
    57541 cagcctcgtt ttttgattaa cgatagttag tgaattgcaa ctcgattggt aagtcaagtt
    57601 tacgtaataa agcaatcaca gcttgtagat catcacggtt cttacctgtc actctaatct
    57661 gatcttcttg gatttgtgct tttactttca gtttttcatt tttgatagct gtagtgattt
    57721 tcttcgcgtt ttcacgatcg attccgttga ccaaatcggc agtttgtcga gctttgccac
    57781 ctagagcgtg ttctgattcg gaaaaatgga tatttttgat tggcacattt cttttgatca
    57841 gcttaccaaa taatacatct ttgatctgtt cgattttgaa atcatcatcg ctcaatacaa
    57901 ctagattgct gttctctaat ttgatttcta ctgtagaatc cttgaagtca aagcgattgg
    57961 acaattcttt tgttgtttgc tgaatcgcat tttttacttc ttctgtattg acttctgata
    58021 caatgtcgaa gctagcatct tttgccatag gaaccctcct gatatacata taaatattta
    58081 ccatacatct atcttatcat aggattaggg cagattctta cctgtttgct aaaattcttc
    58141 tcatttgagt tcgaagtatt ttattttaat agataaactg ttattcttac atttggaata
    58201 ccaaagtagg ggagaggtgc ttgtgagtaa aattatgact gcgtatcata aaaatgaaag
    58261 attaaagaaa atagtggtta ttttgataac cggattgacc gctgcggtcg ctttaaatta
    58321 ttttttgatt ccagccaatg tcttttctgc agggatgaat gggatcgctc aaatcatttc
    58381 ctcgctttta tatgaatggt ttcatatcaa gatagacaca ggaacattta tttttctttt
    58441 gaatatccct gtgtttgtat tagggtttct caaagtagga aaacgagcaa cgatccttag
    58501 ttttgtgaat gttgtatgtg tatcggtgat gacgatgctg cttccagtcg gacagatcac
    58561 tgataatatt ttgatgaatg ccctagtagg cggtgtttta ttaggtcttg gcgtaggatt
    58621 atctttaaaa atgggcttta caactggtgg gatggatatc atctctcttg tactttcaca
    58681 aacaactgga aaaacagtcg gaaactacat gctgattcta aatggtatca ttgtagtcat
    58741 cgcaggcttc ttctttaact gggaaagtgc cttgtatacg attatttcca tttatgcaat
    58801 gactcaagta attgatgcca tccacacgag tcatcaaaaa gtaacagcaa tgatcgttac
    58861 cgtcaagcca gaagaggtaa cagaagaact agcaaaacgg atggtacgtg gaatgacatt
    58921 actgccgtct gtcggtggct attcaaaaaa acaagggaac atgatcatga tggtcatcac
    58981 ccgctacgaa atgtatgatc ttgatcagat cgttcatgaa atcgacgacg atgcatttat
    59041 caatatttta ccaactcatt cagtatttgg ccggtttgca aatgaaaacg aacaaaaaat
    59101 gtatcgaaca actggcgtat tgccagaaat aaaaaatcgt tcacaaaaaa aatgaggaat
    59161 caggtttatt ttagaataaa tttaatctat tacatctaat aagaacattt ttataaattt
    59221 aaacgagaat cttacttcta aattctcatt tacaagcagc taaaaaaaga gtatgataaa
    59281 taaaaatcgt gttaaggagt gtcgtacgat gagcgaagca aaccaagaac ggtctggaca
    59341 agaaatagaa ctaatcaaag aacgtattct tgctgccttg gaaatggtga ttgatcctga
    59401 attaggaatc gacatcgtca atttaggctt gatttatgat atagaattca atcctgaaaa
    59461 tggagaaaca gtcattaaga tgactctaac aactatgggc tgtccattag cagatatttt
    59521 aacagaatct atccatgacg cattaaaaga agtgccagaa gtaacgaaag cagaagtcaa
    59581 actagtctgg tatccagcat ggacgacaga taagatgagc cgttatgcac ggattgcgtt
    59641 ggggatacga tagaaatctt ggctgactag ctatgagaag caaataggaa aaacaaaaaa
    59701 gccttttatc ctttctagaa gaaaagagat aaaaggtttc tttgtttata aaattttg
//
LOCUS       DAYYCW010000005        56809 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000005 DAYYCW010000000
VERSION     DAYYCW010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.005"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(523..1026)
                     /locus_tag="ACHAYA_000279"
     CDS             complement(523..1026)
                     /locus_tag="ACHAYA_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8604116.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1385..2410
                     /locus_tag="ACHAYA_000280"
     CDS             1385..2410
                     /locus_tag="ACHAYA_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8604117.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2733..3611
                     /locus_tag="ACHAYA_000281"
                     /pseudo
     CDS             2733..3611
                     /locus_tag="ACHAYA_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
     gene            4087..4203
                     /locus_tag="ACHAYA_000282"
     CDS             4087..4203
                     /locus_tag="ACHAYA_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8604118.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5131..5469
                     /locus_tag="ACHAYA_000283"
     CDS             5131..5469
                     /locus_tag="ACHAYA_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604119.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5563..5793
                     /locus_tag="ACHAYA_000284"
     CDS             5563..5793
                     /locus_tag="ACHAYA_000284"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM8604120.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6193..6564
                     /locus_tag="ACHAYA_000285"
     CDS             6193..6564
                     /locus_tag="ACHAYA_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM8604121.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6838..7530)
                     /locus_tag="ACHAYA_000286"
     CDS             complement(6838..7530)
                     /locus_tag="ACHAYA_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8604122.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7533..8318)
                     /locus_tag="ACHAYA_000287"
     CDS             complement(7533..8318)
                     /locus_tag="ACHAYA_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8604123.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8555..9955
                     /gene="lacG"
                     /locus_tag="ACHAYA_000288"
     CDS             8555..9955
                     /gene="lacG"
                     /locus_tag="ACHAYA_000288"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM8604124.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9980..10297
                     /locus_tag="ACHAYA_000289"
     CDS             9980..10297
                     /locus_tag="ACHAYA_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8604125.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10310..11983
                     /locus_tag="ACHAYA_000290"
     CDS             10310..11983
                     /locus_tag="ACHAYA_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM8604126.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            11997..13628
                     /locus_tag="ACHAYA_000291"
     CDS             11997..13628
                     /locus_tag="ACHAYA_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM8604127.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(14119..15684)
                     /gene="guaA"
                     /locus_tag="ACHAYA_000292"
     CDS             complement(14119..15684)
                     /gene="guaA"
                     /locus_tag="ACHAYA_000292"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8604128.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15743..16666)
                     /gene="coaA"
                     /locus_tag="ACHAYA_000293"
     CDS             complement(15743..16666)
                     /gene="coaA"
                     /locus_tag="ACHAYA_000293"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM8604129.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            16862..17467
                     /locus_tag="ACHAYA_000294"
     CDS             16862..17467
                     /locus_tag="ACHAYA_000294"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8604130.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            17775..18791
                     /locus_tag="ACHAYA_000295"
     CDS             17775..18791
                     /locus_tag="ACHAYA_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8604131.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            18932..20233
                     /locus_tag="ACHAYA_000296"
     CDS             18932..20233
                     /locus_tag="ACHAYA_000296"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM8604132.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20279..20941
                     /gene="deoC"
                     /locus_tag="ACHAYA_000297"
     CDS             20279..20941
                     /gene="deoC"
                     /locus_tag="ACHAYA_000297"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM8604133.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            20962..21360
                     /locus_tag="ACHAYA_000298"
     CDS             20962..21360
                     /locus_tag="ACHAYA_000298"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM8604134.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21426..22508
                     /locus_tag="ACHAYA_000299"
     CDS             21426..22508
                     /locus_tag="ACHAYA_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8604135.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22720..24282
                     /locus_tag="ACHAYA_000300"
     CDS             22720..24282
                     /locus_tag="ACHAYA_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8604136.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24275..25408
                     /locus_tag="ACHAYA_000301"
     CDS             24275..25408
                     /locus_tag="ACHAYA_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8604137.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25408..26364
                     /locus_tag="ACHAYA_000302"
     CDS             25408..26364
                     /locus_tag="ACHAYA_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8604138.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26463..27629
                     /gene="deoB"
                     /locus_tag="ACHAYA_000303"
     CDS             26463..27629
                     /gene="deoB"
                     /locus_tag="ACHAYA_000303"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM8604139.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27653..28471
                     /locus_tag="ACHAYA_000304"
     CDS             27653..28471
                     /locus_tag="ACHAYA_000304"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8604140.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28488..29195
                     /gene="deoD"
                     /locus_tag="ACHAYA_000305"
     CDS             28488..29195
                     /gene="deoD"
                     /locus_tag="ACHAYA_000305"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8604141.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(29493..30179)
                     /gene="gpmA"
                     /locus_tag="ACHAYA_000306"
     CDS             complement(29493..30179)
                     /gene="gpmA"
                     /locus_tag="ACHAYA_000306"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8604142.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(30351..30932)
                     /locus_tag="ACHAYA_000307"
     CDS             complement(30351..30932)
                     /locus_tag="ACHAYA_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8604143.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(31065..31748)
                     /gene="rpiA"
                     /locus_tag="ACHAYA_000308"
     CDS             complement(31065..31748)
                     /gene="rpiA"
                     /locus_tag="ACHAYA_000308"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM8604144.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            32067..32480
                     /gene="rpsL"
                     /locus_tag="ACHAYA_000309"
     CDS             32067..32480
                     /gene="rpsL"
                     /locus_tag="ACHAYA_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM8604145.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            32564..33034
                     /gene="rpsG"
                     /locus_tag="ACHAYA_000310"
     CDS             32564..33034
                     /gene="rpsG"
                     /locus_tag="ACHAYA_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM8604146.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            33128..35215
                     /gene="fusA"
                     /locus_tag="ACHAYA_000311"
     CDS             33128..35215
                     /gene="fusA"
                     /locus_tag="ACHAYA_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM8604147.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            35387..36574
                     /gene="tuf"
                     /locus_tag="ACHAYA_000312"
     CDS             35387..36574
                     /gene="tuf"
                     /locus_tag="ACHAYA_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8604148.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            36981..37289
                     /gene="rpsJ"
                     /locus_tag="ACHAYA_000313"
     CDS             36981..37289
                     /gene="rpsJ"
                     /locus_tag="ACHAYA_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM8604149.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            37320..37949
                     /gene="rplC"
                     /locus_tag="ACHAYA_000314"
     CDS             37320..37949
                     /gene="rplC"
                     /locus_tag="ACHAYA_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM8604150.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            37977..38600
                     /gene="rplD"
                     /locus_tag="ACHAYA_000315"
     CDS             37977..38600
                     /gene="rplD"
                     /locus_tag="ACHAYA_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM8604151.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            38600..38890
                     /gene="rplW"
                     /locus_tag="ACHAYA_000316"
     CDS             38600..38890
                     /gene="rplW"
                     /locus_tag="ACHAYA_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM8604152.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            38929..39762
                     /gene="rplB"
                     /locus_tag="ACHAYA_000317"
     CDS             38929..39762
                     /gene="rplB"
                     /locus_tag="ACHAYA_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM8604153.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            39805..40083
                     /gene="rpsS"
                     /locus_tag="ACHAYA_000318"
     CDS             39805..40083
                     /gene="rpsS"
                     /locus_tag="ACHAYA_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM8604154.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            40106..40453
                     /gene="rplV"
                     /locus_tag="ACHAYA_000319"
     CDS             40106..40453
                     /gene="rplV"
                     /locus_tag="ACHAYA_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM8604155.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            40467..41123
                     /gene="rpsC"
                     /locus_tag="ACHAYA_000320"
     CDS             40467..41123
                     /gene="rpsC"
                     /locus_tag="ACHAYA_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM8604156.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            41126..41560
                     /gene="rplP"
                     /locus_tag="ACHAYA_000321"
     CDS             41126..41560
                     /gene="rplP"
                     /locus_tag="ACHAYA_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM8604157.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            41550..41738
                     /gene="rpmC"
                     /locus_tag="ACHAYA_000322"
     CDS             41550..41738
                     /gene="rpmC"
                     /locus_tag="ACHAYA_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM8604158.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            41763..42029
                     /gene="rpsQ"
                     /locus_tag="ACHAYA_000323"
     CDS             41763..42029
                     /gene="rpsQ"
                     /locus_tag="ACHAYA_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM8604159.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            42087..42455
                     /gene="rplN"
                     /locus_tag="ACHAYA_000324"
     CDS             42087..42455
                     /gene="rplN"
                     /locus_tag="ACHAYA_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM8604160.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            42492..42800
                     /gene="rplX"
                     /locus_tag="ACHAYA_000325"
     CDS             42492..42800
                     /gene="rplX"
                     /locus_tag="ACHAYA_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM8604161.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            42828..43367
                     /gene="rplE"
                     /locus_tag="ACHAYA_000326"
     CDS             42828..43367
                     /gene="rplE"
                     /locus_tag="ACHAYA_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM8604162.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            43386..43571
                     /locus_tag="ACHAYA_000327"
     CDS             43386..43571
                     /locus_tag="ACHAYA_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM8604163.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            43607..44005
                     /gene="rpsH"
                     /locus_tag="ACHAYA_000328"
     CDS             43607..44005
                     /gene="rpsH"
                     /locus_tag="ACHAYA_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM8604164.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            44037..44573
                     /gene="rplF"
                     /locus_tag="ACHAYA_000329"
     CDS             44037..44573
                     /gene="rplF"
                     /locus_tag="ACHAYA_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM8604165.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            44734..45090
                     /gene="rplR"
                     /locus_tag="ACHAYA_000330"
     CDS             44734..45090
                     /gene="rplR"
                     /locus_tag="ACHAYA_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM8604166.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            45111..45611
                     /gene="rpsE"
                     /locus_tag="ACHAYA_000331"
     CDS             45111..45611
                     /gene="rpsE"
                     /locus_tag="ACHAYA_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM8604167.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            45626..45805
                     /gene="rpmD"
                     /locus_tag="ACHAYA_000332"
     CDS             45626..45805
                     /gene="rpmD"
                     /locus_tag="ACHAYA_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM8604168.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            45849..46289
                     /gene="rplO"
                     /locus_tag="ACHAYA_000333"
     CDS             45849..46289
                     /gene="rplO"
                     /locus_tag="ACHAYA_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM8604169.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            46289..47584
                     /gene="secY"
                     /locus_tag="ACHAYA_000334"
     CDS             46289..47584
                     /gene="secY"
                     /locus_tag="ACHAYA_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM8604170.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            47646..48293
                     /locus_tag="ACHAYA_000335"
     CDS             47646..48293
                     /locus_tag="ACHAYA_000335"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM8604171.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            48484..48702
                     /gene="infA"
                     /locus_tag="ACHAYA_000336"
     CDS             48484..48702
                     /gene="infA"
                     /locus_tag="ACHAYA_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM8604172.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            48735..48851
                     /gene="rpmJ"
                     /locus_tag="ACHAYA_000337"
     CDS             48735..48851
                     /gene="rpmJ"
                     /locus_tag="ACHAYA_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM8604173.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            48869..49234
                     /gene="rpsM"
                     /locus_tag="ACHAYA_000338"
     CDS             48869..49234
                     /gene="rpsM"
                     /locus_tag="ACHAYA_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM8604174.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            49262..49651
                     /gene="rpsK"
                     /locus_tag="ACHAYA_000339"
     CDS             49262..49651
                     /gene="rpsK"
                     /locus_tag="ACHAYA_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM8604175.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            49731..50669
                     /locus_tag="ACHAYA_000340"
     CDS             49731..50669
                     /locus_tag="ACHAYA_000340"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM8604176.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            50697..51077
                     /gene="rplQ"
                     /locus_tag="ACHAYA_000341"
     CDS             50697..51077
                     /gene="rplQ"
                     /locus_tag="ACHAYA_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM8604177.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            51349..52188
                     /locus_tag="ACHAYA_000342"
     CDS             51349..52188
                     /locus_tag="ACHAYA_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8604178.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            52164..53033
                     /locus_tag="ACHAYA_000343"
     CDS             52164..53033
                     /locus_tag="ACHAYA_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8604179.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            53023..53820
                     /locus_tag="ACHAYA_000344"
     CDS             53023..53820
                     /locus_tag="ACHAYA_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8604180.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            54023..54769
                     /gene="truA"
                     /locus_tag="ACHAYA_000345"
     CDS             54023..54769
                     /gene="truA"
                     /locus_tag="ACHAYA_000345"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8604181.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            55433..55627
                     /locus_tag="ACHAYA_000346"
                     /pseudo
     CDS             55433..55627
                     /locus_tag="ACHAYA_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(55815..56591)
                     /locus_tag="ACHAYA_000347"
     CDS             complement(55815..56591)
                     /locus_tag="ACHAYA_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8604182.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            56714..>56809
                     /locus_tag="ACHAYA_000348"
     CDS             56714..>56809
                     /locus_tag="ACHAYA_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HFM8604183.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTG"
BASE COUNT        18147 a        10150 c        12121 g        16391 t
ORIGIN      
        1 taacatatct cattttacac ttttgttatt ttatacttaa aaaataacga cacagaaaat
       61 accgtgtctc agatttctct actacttcta aaaactactg tttttttcct ttgaaatgcc
      121 ttcaaaaagt aggatcaggc agaatatact ggcagaatca aggagaaggg agcctaaata
      181 aggagaaaaa ccatagaaaa tataaagtaa ttacttataa aacaagcttt ttatagaaaa
      241 ataatatacc tgagtagggt ataaattaag acggaattcc tttctagatt gagatattga
      301 ctaggaagaa tgttaagatt ttaagccttt aagctaaaaa aaaacccttt ttgttaaata
      361 aggggaatat agcctgtcag tttcaaactg caagtcgtcc tgctgcaata atatagtaga
      421 ttagagatga aaatagattt ttttaaacca atgactgaat aaaaagaccg tcatagatac
      481 acattggaat gtgtctatta cggtcttttt ttacgaacga ttttattgaa gacggacagt
      541 tcgggtcaaa aataaaaaag ctacgatcag aaataagaat aaaatgtatc ccaaaataat
      601 ggctagacgg tctaacttat ttgtcgatag atgtttgttt agaagttgat aggcccataa
      661 acctatcagt cctcctgttg tattagttat caaatcagtg atgtccgcta cgccaatacc
      721 gaataggaat tgaatcaatt ctgccaagaa gcttacaagg aaaataatcc ctaatttttt
      781 agaaaaagaa agtctcttga aattgacatt caaaagcacc ccaaaaggaa aaaagacaat
      841 caagttataa gtggtctcac gtaataccgt cgtattttca aaagaaaaag tagaaaaagg
      901 gacgagattg acattgctgt aagaataagt gagaaattgt ggaagattaa atgatagctt
      961 gaataaaatc aaccataata gaaagaataa ataaatccct aataaaaatt ttgatacata
     1021 ccgcatagac atctcctcca tttaaaaagt ataccatcga aaaccgctga aaaatcataa
     1081 aaaagtatga agtttaagga ccaacgtagt tatcgtacga aaattagaag cttttttttg
     1141 ttgggaatcc ggtttcttta acaaagctaa gatagcgagt tgcttggaaa agttgctatc
     1201 ttttttctgt caaaacgact cggaatgttc gttttttgtt ctaatttctc caagttgttt
     1261 actttttgat actaagttta tgaaaaatca agaaatagtt gttatactag gtaatgctga
     1321 caaacgggat tggcatttca tcttggttca gctttttttc caattactag gaaggaggga
     1381 tagattgttc aagacttatt ttcgtttcaa taaaatacaa gataatcctt ttcgtatcat
     1441 gaatctgatg ctacttatct ttattgtatt atggttaggt tacataaatc atgatttttt
     1501 gatttcaagt ttttcatcat tagggatctt tacttttttc tattatcaaa acataccgat
     1561 gaaatatttg ctgaaacgta tgtttctgat cggttgcgga ctgttggttg ctttcgtttt
     1621 ggggatctta tcgacttatg ttttatggct tgaaccattt gccgtagctt tggttgcttt
     1681 ttccagcaga ttcgtgttgc gattattcca tatttctaaa ccaggtggct tgttttttgc
     1741 gatgctttct gccatgggaa cgagcatgca gctgccaatc gctcaattac cgatcgtctc
     1801 actttatttt tttatgggtg tcgtttttgc cctgattgcg gcagtgatta caaaattact
     1861 ggattcacgg cctgagcaga caattgaaaa agcaacttta aaagaaagat ttcacgaaga
     1921 gcctttagtg atcattgatt ctgttttcta cagcgctgcc ttatttcttt ccgtttatgt
     1981 cagtcacggc ttgaatctgc ataatcctta ttggttgacc ttgtcatgtg catcgatttt
     2041 gctagcagaa aacttggatg cgatgaagca tcgacaagtc caatatctga ttggatcgat
     2101 gggtgggtta tgtgtgtcgg cattcttgtc atttgtgccg tttacgcagt tgcagaccat
     2161 ttttctgatc acttttctat atggaatcgc acaatttcta gttgctagaa attatgcagt
     2221 tgctaatatt tttcttaatc cgatggcgtt gatgctatct acacttattc gtggagcata
     2281 cttgatttct ttgatcgaat atcgtttttt agggatcgtc ataggaagtt tcatcggttt
     2341 aggtgttgcg tgggtaatga cggttggttt gcaacattat ttaacggttg ttcgtcaaaa
     2401 attagaatag aaggacaaat atatgtgcca aaagaggtta tgacaagatt tttgtcacaa
     2461 cctctaattc aaggtgttca aaagccagat tctcagcatt aagtcaaatt cacacaaaaa
     2521 tgtggaaagg aactttcaaa aaaattttat atttattcta tcaaaaaatt ttgtttatga
     2581 tggtaactga ataaactttc ctgagatggt caatgctttc cttgaaagtc ttacagatat
     2641 tctttcttca tagaaagttt aagttttata gttagtgtac aataaaagta tgtttatacc
     2701 atatggtgtc cggataatca gtcatttaaa caatgaggtt caaagatatg aaaaggggaa
     2761 atttgatgaa gaaaataagc aatgaaatcc aaattctggc agaaacttta actcttccca
     2821 aagtcgttat agagcaaatg attgtacaag caaaaaacat ccttgatgag gtgtttgagc
     2881 aatatagtca tgaactaatg gatcctaata aagcaaaggc gactgcaaag aaaatagaca
     2941 aagtctatga agaggaacct tttgcagcat tagctattta tctatatgca gcttctcaaa
     3001 gttggaaaaa aatttatcaa ccattgaaga ttccaagaac tatctatttg gcaacaatga
     3061 atgcttttac tagatttatt catgaacatt tccaagtaac aggaagctat cgctttgacc
     3121 gtggtttttg gatttggcat tacactagtg gcttgatctt tcggattggt gaattggagt
     3181 acgaaatgac atattttccg aaagggtaca cggatctaag attagaagga aagcagtgcc
     3241 tttctcttca tattccttcc gatgcagatt tatctcagaa taagataaga gatagttacg
     3301 aaagtgccat tcgttttttt gaactttatt ttcctgatta ccattaccaa gtgatgtaca
     3361 cagatacttg gttactatca cccaatctta ctaaatagtt gaaaaaagag tcaaaaatct
     3421 gtctttttgc agccgattat cgattattat cagttgatga acaggacgat agtggagtcc
     3481 cttggatctt tggacgagta gatgcccaaa tacatgatta tccagaaagt acgagtttac
     3541 agcgacaagc aaaagagcaa ttgcttgcag gcgagcacat tggctctggc ttaggtattc
     3601 ttacttcata agtttagtac ttcgaaatag tttaaaagag cgtgagacaa aagtaaagaa
     3661 cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagcaa ctccttcgga
     3721 aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaatttctt cttatttctt
     3781 ggagttaaac gcttctgtcc cagcctcttt tttagtgttc tatcgaacac acagtacgtt
     3841 caataggtaa actagctata acaatagata ttaaagttaa cggacaagag ggcctttttt
     3901 aggtagctaa aacgtaataa tgaaaattta tttccagaga taggagtcaa tcagctagtc
     3961 tgatcgactg ttttaacaag ttgactttca ttttaacttt tgataccatt taaaacacaa
     4021 atcgtaattg tttcgatttg tgttttttag catgactaca atcaaagaag aaagtgggtc
     4081 gtccatatgg aaaaaacaga tatctcaagt gcataccgcc gcttgaaaag cccaaatatc
     4141 aaaacacgaa aacgtgcgct gaagatcatc aaagatgtga aacggaatag tgggaaaagg
     4201 taagaagaag gaaacttaat taaaaaaata gccgaaaaac gtttttcggc tattttcaat
     4261 aatcggtttt tgtgtaaggt ttccgaaaga actgctgttg attttccatt tttaacatat
     4321 gataaattgg tagaatcttt cttttcaaat tgataccatt ccctaattcc agttatatag
     4381 ttaactacag aaccacaaag cacgtacgtt tataaaagta ttatctgtag ttatttttca
     4441 attccctttc aacctcttat tttgaccagt agtattgatc actttacctt ttaacacctt
     4501 tgttcgtttt tgaaacattt ttagtcaaga atggaaacgt aactacataa tcaaaggttt
     4561 tgcgaaatat ttcatgaaat atttcatgta gaagcgagca atacggttgc aaataaccat
     4621 tatcgtttaa taagcagtta cgaaagctcc caatatctct aacgttcagc tcactccatt
     4681 tcttttcttc aaaaaaatca gtgattcgta tctctttcta tttgtcgaaa aagttaactt
     4741 taaaaagtaa cgccgaaaaa atatgcaaaa aaataaaaaa gcagttacta ttgctagaat
     4801 aacatcaagg aataataagt tttggttttc agataatagt cgataagttg tgtattggaa
     4861 ttttagatac agtaataaag tatttttcat gaaattttgt gagaatcact attacaagta
     4921 tctttaataa aattaatggt taaggaggaa cttaaagtag aatgtaaatg aatagttgta
     4981 gtcagtcgat tgtgatacta gactcaaagt tgcaggaata agtttttcaa tttgtgattg
     5041 gaagataaaa aatgaaaaag aagggctttt ttttatactt tgttgattgt agtgtttatt
     5101 ttccctctgt ttagccaagt aacattttct atgactcaac cagtttcaag tgaggaacat
     5161 gataaccttt ttcgtgctac agaagagttt aagaaatatc aggaaaatat tgttccggag
     5221 actccattag ctagtgagcc aattactgat attgatggaa accaaatagg atggtatata
     5281 cagtacgaag ttatggtggt aaataatgac tcaatagtaa aaaatacagt aggcttagag
     5341 ctaacaccga ttgaaaaggt gagttcattg ttaacgtata tttatactga tgaaagtgac
     5401 gagactgaga tcattttaac ggattactca aaaattgcta ctgataaaaa gtatatattg
     5461 tggatgtaga cactagtgaa atactttatg atatagaaat tgatgaaaat ttactagatg
     5521 tagcacaaga aatcgatcat caaaagcagg aagctattcg atatgctggt agaaaaggga
     5581 aaagaaaata tttattacgt gaatgtagca aaagttagag aagatgaaaa tgagtggaaa
     5641 gaattcaaga gtcgctactc aattaactcg actccgacat ttacagttta cagagaagga
     5701 tcgatagaaa aaactgtttt ttggactaaa gaaagtggaa tgtctttagc tgaagtagaa
     5761 gagtttttgg attatgttag tatgcaacag taagggagtt cttattatga aaaattttga
     5821 cacatattat gttattgttt tttcgatatc tttgctgtta atattactaa ctttaatgca
     5881 gtataaagag tatccagagt attttatttt aacttttgta tatttagtaa gtagctacac
     5941 acttctatat aaattcagaa gttcttctag agtttctaaa gtttgtttct taattagctt
     6001 attgattcta taatattttt gatttatata ggaaattaaa aatattatag aatcaacact
     6061 cagagaacgg gttggttttt aatagattta ataagtactg ataatgaaat ttctcctggt
     6121 tgggaaaacg aagttggtta ccctgtagaa gaattttttc aagattactt tgcggagtat
     6181 gccgatagga taatgagaga ttacgtaaca gataaaactc aagaagtcat caatcaatcc
     6241 cagtcattaa atgaattatc tgataaggcg aaagaagaag cagttgccat tatttcttca
     6301 gggattagtg actatattga tatgagtggt gaaatcaatt atggcgataa agacaaaatt
     6361 gtacgcatga taaaagcttt tatcaaaact tttgatgtga aggcagatct taagtttaat
     6421 gatacttacc tagtaggaaa attaattaat atccttggga cagagaaaga tacggaaacg
     6481 tttatttcct ctttgtctat gttgtttaca gataagcatt tttcaactca gcttggtgga
     6541 gaggaactag tggaaatctt ttaaccatta cgcccagcat taataaaagt atatcaaaaa
     6601 agtactcctg atgattttta agattggttt gagaaataat gtgaaaacac tatttttaat
     6661 gttaattata gatagagatg aacgcgtcat atggttaaaa gaatgaaaaa gtcaaagata
     6721 cactgctata tttcactaag gatatagcag tgtatctttc atgtcgtttc actggcagtt
     6781 ttttataaaa ggaggtgtgc atttcagttg cttttctttg gtagttagtt gaatcgttta
     6841 agaattaacg gatgtggcag acaggtgctc tgtccaaaaa ccagtaaaat ctttgatggt
     6901 tccatcttta tttagctgtg cctgttcaaa tactgttttg tttttaccga atggatcaat
     6961 tgcaataatt gttccgatcc ccgcacgttt ggcagcaagt aatcctgagt aagcatcttc
     7021 gataacaaga caatcagccg gagctaagct cagtttgtct gccgctttaa gaaaaatatc
     7081 cggttgcggt ttcccaggga aagagccgtc gtcatagaca atcttgtccc agtcaaacca
     7141 gcgggctaaa tcgaaaatat caaaataaaa agcaacattc tctttgacag tagccgtggc
     7201 aatcgttaat ggaatctgtt ggtcagtcag ctggtcaaga gtcgcttcaa gcccttttgt
     7261 caactgaagc tcgttttcat tttgcaagca aagcatacga taatagcttt ctttttcttc
     7321 tgacaatacg gcgatctcag aggcggtcaa atgctctgaa atgaaatgtt tcaaaatctc
     7381 cgtattcgtc cgaccatgaa tattttttaa aatatcctcg tctgataaac catcttttgt
     7441 atgatcatgt atcatatgca gccaagcagc ttcatgcaga tgagagtcca aaaacattgt
     7501 tccgttgaaa tcaaatataa tccctttcat agttacgctc ctatagtggt attttcggtt
     7561 gaatcagcaa tttgcagatc gacatattgt gcatagtgct cttgaacctc ccgcgagatc
     7621 tggcgatccg taatgagcag atcgaaatca tgcaaattat aaaacacata aaaatcatct
     7681 cgattgaatt ttttattatc aacgagtaga tatttcttac gggaattatt tagagcgatc
     7741 tgctgtgctt ctccttcttc ggtactgtaa gtagagatcg cttcgttgtg aacgccattt
     7801 gaactgataa atgcttttgt aaactttagt cttcttaaat tgtcgttggt gatcggtccg
     7861 acaaatgcgc cggtattttt ccggaaatca ccgccaatca gtagaatttc tggtccatct
     7921 gcttgagcaa gaatatcaaa aactggataa ttatttgtaa tgatccgaat tttcttgttc
     7981 agtaaatgcg tcgccaatag ttcgattgtt gtacctggtc ctaagaaaat cgtttctccc
     8041 tcgtgaatca aggaggctgc tgtggcagca atttcttttt tttcttgaat ctgcagagtg
     8101 gctttttcag tgtgagaaag ttcttgatct aaagtgtagg aaagactttg agcccctcca
     8161 tggacacgca gcagcttgcc cgatttttct aattcatcta aatctctgcg aacggtcata
     8221 tcagaaacat ctaattgatt gatgatttca ttaacggtaa ggattccttt tttattgacc
     8281 atttctacga tagtaagcaa tctttctttt ttcaacattg tcttcacctc taatttaagg
     8341 ataatctatc atataaacgg acaacagtca atctaattcg aacaaaagta aacatttagg
     8401 gtggttttta tgtttgttac aatagatttt attgctgttt tctggttttt atgattattt
     8461 gtgtttgttt ttgttgacaa atgttttgga tgcgtttaca atgcaatcag ttagaaagag
     8521 cgaaaactga ttatagattt gaggagagat gtacatgctt aaattaccag aagattttat
     8581 ttttggcgga gccacagcag cctatcaggt tgaaggagcc actaaggaag gtggcaaagg
     8641 cgctgttgct tgggatgact ttttagagga acaagggcga ttcagtcccg atccggcaag
     8701 tgatttttat catcaatatg caaaggacat tgaattgtgt gaacgttttg gtgtaaatgg
     8761 tctaagatta tccattgctt ggagccgcat ttttcctgat ggagctggga aaccaaatcc
     8821 agaaggaatc gctttttatc atcgtgtgtt cgaagaatgt aagaaaagaa acgtaacgcc
     8881 gtttgtgacg ttgcatcatt ttgatacccc taaacgtttg tttgaccatg gagatttttt
     8941 gaatcgagaa acaatagaag catttgtttc ctatgccatt ttttgttttc atgaattcaa
     9001 agaagtcaag gtgtggagta cctttaatga aatctatcca gttgcaacca atcaatattt
     9061 gttaggtgtt ttcccaccag gaatcaaata tgattttaca aagatcgtcg cctgtcttca
     9121 taatatgatg gttgctcatg cccgtgtagt gaactatttc aaagaaaacg aacttccggg
     9181 tgaaattggt gtagttcact cattagagac gaaatatgcg gcaacagacg cgccggaaga
     9241 taagcatgct gcctttttag acgatgcatt gtcgatccgt ttcttattag atgctaccta
     9301 tttaggctat tattcaactg aaaccttgac tgctcttgat gagatatgtg aagcaaatca
     9361 ggccagctat cattttcctg aagaagattt cgttgaattg aaaaaagcat ccacgcgtaa
     9421 tgactactta gggatcaatc actaccaatg ccattttgtc aaagcttacg atggagaaaa
     9481 tgcaatccat cataacggta cgggtgaaaa agggacctct gtttataaag tgaagggaat
     9541 cggagaacgt atttataaag aaggaattcc tcgaactgat tgggactggc tgatttatcc
     9601 agaaggtcta tatgacttgt tgctgcgaat taaaagcgat tacccacatt acaacaaaat
     9661 ctatatcact gagaacggga tggggtataa agatcagttt gaagatggca ttattatgga
     9721 tcagccgaga atcgactatt tgagagtcta tttggaatcc ttaagcaagg cgataacagc
     9781 cggggtaaat gtgaaggggt attttttgtg gtcgcttatg gatcttttct catggacaaa
     9841 tgggtataac aaaaggtatg gcttgtttta tgttgatttt gaaacccaga agcgttaccc
     9901 aaaagaatca gcttactggt acaagttggt cagtgaaacc aaaacgatta tttaataagt
     9961 gataatggag gaagaaaaga tgaataaaga ggaattacaa atgcttggtt ttgagatcgt
    10021 tgcatattca ggcgacgctc gcagtacatt attgaaatta ttgaaagaag taagacaagg
    10081 aaactttgaa catgttgatt cggcgttgaa agaagccgac gaaaatttaa cccttgcaca
    10141 taattcgcaa acaaaaattt tagcggaaga agcatctggg aaagagatgg agatgggatt
    10201 catttttatc catgggcagg atcatttaat gaccacttta ttgctgagag atctgattca
    10261 agactttatt gtactgtaca gacagaatca aggataggag aagaacgcta tggatggaat
    10321 cattaaacaa atcgaaaaag ggaaaccatt ttttgaaaaa atctcacgta atagttattt
    10381 aggagccgtt cgtgacggat ttttaacagc gatgccagct attttatttt caagcatttt
    10441 cattttgatt gctgcggttc ctgagatttt tggttaccaa tggcctgaat ctgtctccac
    10501 atggctatgg aaagtctata attattcgat gggtgttgta gggttactcg tttcagcaac
    10561 cactgctcgc tgcttggctg attcgatgaa tcgtcgctta ccgaaaaaca aagtaatcaa
    10621 caccacctct gtgatgctgg cctctgtggt aagtttcctg ttgttaagcg tctctccgat
    10681 tgaaggtgga ttttcaacgg aatatatggg gacaaaggga ctactggctt cctttgtcgc
    10741 tgcattttta acagttaatt tatataagtt ttgtgtgctg aaagatatta ccattaaaat
    10801 gcctaaagaa gttcctggaa cgatttcgca aaccttccga gatatttttc ctttctcttt
    10861 ttcagtattt gcggccgttt taattgattt aggcattcga caaatcttcc aaatctcatt
    10921 tgcagaagca atcattacgg tgctgcagcc gctatttaca gcagctgacg ggtacttagg
    10981 aatcgctatt atctggggag cgatggcgct gttttggttt gtcggtgttc atggaccgtc
    11041 gatcgtggag ccggcaattg cggcaattat ctatgccaat gtggaaacca atctgcaatt
    11101 gttcaattcc ggtgaacaag cttctaaagt actgacggtg ggtttaggga attttgtagg
    11161 aactatggga ggaactgggg caacattggt tgttcctttt atcttcctac tctttgctaa
    11221 gtctaagcag ttgaaggcag ttgggaaagc ttcctttatt ccagtaagtt tcgctgtcaa
    11281 cgaaccattg ctatttgcgg cacccatgat tttgaaccct tattttttta ttccattttt
    11341 gttaacacct gttgtaaacg tttgcctgtt caagtttttt gtcgatgtat tgggcatgaa
    11401 cagctttatg tacgttttgc cttgggcaac tccagcaccg atcggtttgg ttctaggaac
    11461 gggcatgggt atcttagctt ttgttctagc agcggttcta atcgtcgttg acttcctgat
    11521 ctatctacca ttttgcaaag catacgatgc tgtcttagta gagaaagaag cacaaaaagc
    11581 agaggtgatc gcaaccgagg aaggtgcttc tgccgatgta cgtgaggaag tacctgtggc
    11641 agcgagtcaa gttatcagcg atgcaaataa agaaatcaat gtgttggtac tttgtgctgg
    11701 cgcagggaca agtgggatgt tagctaatgc tttaaaaaaa ggtgcatctg aatcgaaagc
    11761 accgatcagt gcaatggcgg gtgcttatgg ttctcattac gaaatgctgc aggattttga
    11821 tatggtggtc ttagcaccgc aagtcaattc ttactatgat gatatcaaag aagacaccga
    11881 tcgcttagga atcaagctgg ttgcgacaaa aggagcagaa tatatcggat taacgagaga
    11941 tcctgaaaaa gctgttcagt ttattctgaa ccaatttcaa taaaggagga gccataatga
    12001 aaatgggaat ttggttgcgt aaagctgttt tatttgcagc tgtgttaggg gctttggggg
    12061 ctgtttcaaa aactgttgaa gcatctgcca gcgaatttat tcgcggagca gatatttcga
    12121 tcctgtcaga tatggaaaaa tctggtgcaa aatattatga agacgatatt tcaaaagatg
    12181 ccttagagat tttaaaaaat aatggtgtca actatgttcg actgcgtttg tggcaggatc
    12241 cttataatag taatggagaa agttatggag cagggacaaa tgatctggaa acgacgattg
    12301 accttgcaaa acgagcaaaa aatctgggac ttaaggtgct gctggatttt cactacagtg
    12361 atttttgggt tgatcctggg aaacagaatc ttcctaaagc atggcaaggt ctgacctttg
    12421 aagaaatgaa tacggcattg tacgactata caaagaacgt tctgtcggaa atgaagcagc
    12481 tggacgttta tcctgatatg gttcagatcg gcaatgaact gaatagcgga atgctctggc
    12541 cttatggcaa gtcttgggga gaaggcgggg gagaatttga tcgattggct gcttttttga
    12601 agtctggtat ccaagctgtt cgagacactc aacctaaaac aactccagta atgctccact
    12661 tagctgatgg tggcgataca ggagcattta cttggtggtt tgatgaaatc acgagtcgag
    12721 gtgtgtcatt tgatttaatt ggagtgtcgt attatccgta ttggcacggt tcaatgaatg
    12781 aacttcaaac caatatgaat gcgatcagcc aacggtatgg taaagaagtg atcgtagttg
    12841 aaacagctta tgggcataca acagcgaatg ccgatactat gccaaatgct tttggtgaag
    12901 ccgaagcagc tgcgggggga tatgaaccaa caccgacagg acaagcagag tatttgctgg
    12961 atcttgccga tcggattcag gcagtaccga ataatcgcgg ggcgggcttc ttctattggg
    13021 aacctctttg gtacaacgga aatgtgagtt gggcaacaca agcaggtatg aattatctca
    13081 gtgttcaatc cactatggga aacgaatggg acaaccaagc gatgtttgat tttgctggaa
    13141 acgccttgcc aagcttgcgt gcgtttaaac aagcaggggc gcaaataaat ctagttaaaa
    13201 atgcaagttt cgaaagcgat ggaccaacga ccagtccttc caattggcaa acctggtttc
    13261 aacaaggaac cgctaagggt acgatcaaaa cggaagcggg cacttttttt ggggactaca
    13321 aactaacttt ttggcaggat caagcatacg aggcatcagc ttatcaaaat gttagtggca
    13381 taagcaaagg aacttacaca ttgagtgcct atgtaatgtc tgggggagag tttgatacta
    13441 atcagttata tatcaaaaat tatggtggag cggaactaaa tactgcggtt gttaaaacaa
    13501 gtgaatggac aaaaattaca atcaaagaca ttccaattac aacaggtgtt tgtgaaattg
    13561 gggtttatag caaggcttca ggaaatgctt ggacgaacgt tgattttgtt tcattggtga
    13621 aacaatagac tgcggatcgt ttcgattcaa gatcggaggt tcccttcagc cacatcgtta
    13681 gcgtagaagg caggaatcga acctgcacct cgcctttgcc aaaaataacc agaaacgatt
    13741 tccagtcatt tttaccaaaa cgagtaaagc accagacttc atctacactt tgtattatgc
    13801 taaagagagt tcaacgattt cgtaagaagc aatgaaaaaa ttgcaaaaag ttgtgcagat
    13861 aacataaagt ttgtttaaga aagggtaacc ttagaattcc gtcgtgaata tgatttgagc
    13921 acttagatgg atagaaaaaa gttctcggaa aacagttcgt ttttcgagaa cttcttttta
    13981 tgataagaat gaaacaaatt aacttatatt atagtctctt ttggtaggga accatcttat
    14041 ttttaatgaa aatcaagcat tttagctatt gaagaatgtt gatttatagg gattccccct
    14101 ccatattatg ttggctcttt attcccactc cactgttgct ggtggtttag aagtgatgtc
    14161 atacacgata cggttgacgt gggctacttc gtttacgata cgtacagaga ttttttgcaa
    14221 gacttcccat gggatacggg caaagtcagc ggtcattccg tcgattgaag ttacagcacg
    14281 gataccgact gtgtagtcgt aagtacgtcc gtcacccata acaccgacag agcggatccc
    14341 tggaagaaca gtgaaatatt gccagatatc acggtcaagt ccagcagctg cgatttcttc
    14401 acgcaagata gcatcagatt cgcgtacgat ttctagcttg tcttcagtga tttcgccaag
    14461 aacacgaata ccaagaccag gaccagggaa tggctgacgc caaacgattg aatcaggcat
    14521 gcctaactga gtccctaatt cacggacttc gtctttgaat aatgtgttta atggttcgat
    14581 caattcaaat tgcatatctt ctggcagtcc acctacatta tggtgagatt tgatcgtctg
    14641 tgctgtttct gtaccagatt cgataacgtc agtatacaac gttccttgtg ctaggaaaga
    14701 gacgccttct tcaccagcaa gttttgttgc ttcgtcatca aaaacatata cgaattcatt
    14761 cccaatgatt ttacgttttt gttctgggtc agatacacct ttcaacttgt ctaagaagcg
    14821 atctcttgca ttgactttga tgatgttcaa gccgaatttt ccgcctagac tttccattac
    14881 ttgttccgct tcgcctttgc gtagcagacc gtggtcaaca aagatagatg tcaattggtc
    14941 accgatcgct ttttgtaaaa gaaccccaac gacactagaa tcaacgccac ctgaaagacc
    15001 aagcaagact tttttgtcac caacttgttc acggattttt tctacttgca tatcgataaa
    15061 gttatccatt gtccagttac cttcacagtg acaaacatca aaggcaaagt ggcgcagcaa
    15121 ttcgattccg tattcggaat gacgtacttc tgcatggaat tggataccat aaaaattacg
    15181 tgttgtgtct tggatagaag caatcggaca atctttgctt gttccgactg tttcaaagcc
    15241 ttcaggaact ttagttacca agtcaccatg gctcatccat actgtttgtt gttttggtgt
    15301 ttctttaaac aagacagctt gatcgcttgt tacttctaac aaggctttcc catattcgcg
    15361 gtttttagca ggctctacgg aaccgccaag tttgtacgtg atcaattgca taccatagca
    15421 aatccctaat acaggaatac ctaaattgaa gatctcagga tcgatcccaa atgcattctc
    15481 atcatataca ctgtttggtc cgccagaaag aatgattccc tttggattca tcgcttttac
    15541 ttcttctgca gtaatgcggt ggctcaataa ttcggaaaag actccaagat cacgaatacg
    15601 tcttgtgatc aattggttat actgactacc gtagtctaat acgataatct tttcaacgtt
    15661 cgtcatatcg gcaacgtttg tcaaatttat tacccctatc cctttaaatt ttatctcaaa
    15721 atagtcaaac tattttaaaa tgctaatatt ttcgcatgaa aatcgaatcg atgatatgat
    15781 tttcagtttt atggagaatt atatcggctc ttcctcgtgt aggcaagata tattcctgta
    15841 aatttttcaa attcacattt ttccatacat cttttgccat tttaaaggct tcttcacgat
    15901 ctccgatagc aaaacgataa taataattct ctggattttg gaaagccgta tccagtaacg
    15961 caccaaaacg atccaaatac catttttcga tcagcacggg atcggcatcg acatagacag
    16021 aaaaatcaaa aaagtcgctg acgtagattt gctgattcgc aggtaattga agcgtattga
    16081 tcccctcaac gatcaagata tcaggctgct ggatcaattc gtacttgtct ggaatgatat
    16141 catacacatc atgtgagtaa acaggtactt ttatctcttc ctgaccagat tttacacggt
    16201 tcaagaaatc gatcagcatc tccatatcat aactttcagg aaatccttta cggtccatga
    16261 ttccacgctc ttttaatata cgatttggat aaagaaatcc gtcagtcgtg atcagctgaa
    16321 cgtttcttcg tttgaaggta cgagacaaaa tcgtctgcag cagtctggct gttgtacttt
    16381 ttccaacggc tacacttccg gcgatcccga taataaaagg cggtacttct acgtatttat
    16441 gaagaaataa acctttactc aagaacaaag attcaaattc tttcatataa agataaatca
    16501 gatgagcaag aggtacatag atatctcgaa catcctgcat ggaaatgcgg tcattcaaac
    16561 ttttgatatt atctagttca tcttgtgtca aaggtgcttg cccattacga taaaattctc
    16621 gccattcttc tcttgccaca cggtagtagt tcattggatc attcatatta gcctcctgcg
    16681 ttctcccatc aatggaacta ttttaacaca aaataaacgc aaaccccacc acttaagaga
    16741 gattgccgat ctttttgtat aatctttata atagaaagaa acggagtctt attcttgact
    16801 tgcgcacgga tacataaaag gatagactgt tgtcatgaaa gaaatgagaa ggatggaaga
    16861 catgacaaat cattactatt ctgaacgccc agatacggcg cacgattttg aacaatgggc
    16921 ttttgagtta aaaggcagaa cctttcaatt tgtaacagat tctggtgtat tttcacgtga
    16981 aacagtggat tatggttcac gtgtcctgat tgatgctttc gattgggaag agttacctga
    17041 tgaaggaaag atcttagatg tgggatgcgg gtatggaccg attgggttag ctttggcatt
    17101 tgcgacacag cggtttgttg aaatggtcga cattaatgaa cgagcggtaa gtctagcgca
    17161 aggaaacgca aagcgtaacc aaatcgaaca tgttgatatc catcaatcga atatctatga
    17221 agcagttcat gaagaaacct atgcagctat cgtaagcaat ccgccgatcc gtgctggaaa
    17281 aaaagttgta catgaaattt tgacaggcgc atatcctcgg ttgaagaagg gcggtacact
    17341 gacaattgtg atccagaaga aacaaggagc gccgagtgcg caaaagaaaa tggaagaaac
    17401 atttggtaat gcagaaatcg tcacaaaaga taaaggatat tatatcatca aaagtgtgaa
    17461 agaataacta gctgaaaaat agtggagcga cgaaagagtg gaactgggcg tgcatgtacg
    17521 ggtacgtatc agttcttttt ttgttgagaa gtttttaaaa acaaagaact attttttctt
    17581 caaaaagaaa tagtaggaac agagtcgttg ttttttacga taaaataaat aagatacaga
    17641 acttgtatag aaggctgcca agtcggtaga taaaggaggt ttttatcaaa aaacatactt
    17701 tccttttttc tctattcttt tcttgggggt tttggtacac tgatagggaa tttagataga
    17761 agggagccaa taggatgaag ctgaccatca gagatatcgc agaaatggca ggcgtatcag
    17821 taacaactgt ctcacagatt ttgaataaca aaggaagcag atttagtgat aagacaagaa
    17881 aaaaagtgtt gtcgatcgtg aatgaacatc attataagcc cgattatttc gcttcaaatg
    17941 tgatcaatcg acattcaaag accatcggaa tgatcgtccc tgatgtcact gatttcttct
    18001 tctccaaact gatcgaaggt gtagagagct atttgaatcc tttgggctat gtgatcatgt
    18061 tatgcaattc tcgtcatagc caagaaaatg agatcaagta tttacaagag ctttcccacc
    18121 gctcagtgga cggaatcttg ctggccacac caaatatttt acctgagaaa tatgcattag
    18181 acagcggttt ttatcaaaaa atgccaatca ttttaatcga tcgcggattg aatcgtcggg
    18241 ataacggacg gcttatcgta aaggaatatg agggcgccta tcaagctgtt tcgctgttta
    18301 ttgaaaatgg ccatacgaag atcggcatgc tgaaggaaac cactggatac tatcaattag
    18361 aagaacgttt caacggatat cgtcatgcgc tgaaagatca tggcctgcct tttaatggga
    18421 aatttgttga acatggagag ttgactgtcc aaggtggata tactgcatca aaaaaattac
    18481 tcaaacataa agatatcacg gctatttttt gcgggaatga tgccatggcg atcggatgct
    18541 atcaggcgat cgatgaattg ggcaaaaaaa tcccagaaga tatctcagtc ataggattcg
    18601 atggattaaa gctgtcagaa tacatgattc cgcaattgac gacagtgcag cagccaagtt
    18661 ttgatattgg attttatgct gcacgtttcc tgatcgatac gatcgaattt cctcaaagaa
    18721 aagttccgaa caaagtgttc gaaacaaaat taattattcg tgagagcgta aaagtattga
    18781 caaagggcta actcggcggt atattgctta tgtaagcatt tacaaaggct agttagtgct
    18841 ttttttattt ttagtaaacc agtttactaa agtggtttac ctttcctaaa aataatgtat
    18901 aataccgaat gaagacaagg aggatcaggc aatgcgaatg gtggatctga ttgaaaaaaa
    18961 gagggatgga aacgagctgt cgaaagagga aatcgagtac atcgtcacga actatacgaa
    19021 tggaaagatt ccagattatc aagtcagtgc attattgatg gcaatttttt atcaagacat
    19081 gacaaatgaa gaaatcacga acttaacatt ggcgattgct aattctggtg atgtcatcga
    19141 tttgagttct cttgaaggaa taaaggtaga taagcattcg acaggtggcg ttggtgacac
    19201 gaccacattg attttagccc ccctagtagc tagtgtcgga gtgacagtgg ctaaaatgtc
    19261 tggacgcggt ctaggttaca caggtggtac actagacaag ctagaagcaa ttcccggatt
    19321 tcagatcgag ctttcggatg aagaatttgt acgcattgtt aatgaaagta aagtagcggt
    19381 aattggtcaa tcaggaaact tggcaccggc ggacaaaaaa ttgtatgcct tgcgtgacgt
    19441 cacagcgacc gttgattctc ttccgctgat tgccagttcg attatgagta aaaaaatcgc
    19501 ggcaggagca gatgcaatcg ttttggatgt gaccactgga gacggtgctt tcatgaagaa
    19561 catcgaagat gcaagacgtc tggcaaagac catgacgagc attgggaaac tggctaaccg
    19621 agaaacagtg gcggttatct cagatatgtc agaacctctt ggcgaagcta tcggaaatag
    19681 tctagaagtt gtcgaggcaa tcgaaacatt acaaggaaat ggtccagaag atctagtaga
    19741 aatgtgctac gccttaggaa gccaaatggt tgttcttgct ggcaaagcta agacaaccga
    19801 tgaagcaaga acgttgcttc aagaagctct ggaatcaggt aaagcgttag caaaattcaa
    19861 agaaatgatc caaaatcaag ggggagaccc agcgatcgtg gaacatccag aacgaatttt
    19921 gactgcacgc tacacaatgg agcttcctgc taaacaaagc ggagttgtgt caaaaatcgt
    19981 ggcaaatgaa ttaggaattg ctgcaatgat gctaggtgct gggcgtaaaa ccaaagaaga
    20041 agatattgat cacgctgttg gattgaaact gcataaaaaa attggtgaca ctgtgacaaa
    20101 aggtgagtca ctattgacga tctatagtaa cgacgaagaa atcacttctg tcattgaatt
    20161 attatataaa aatattgaaa ttggtgaatc ggcaatgaag ccaacgttga ttcatgacat
    20221 catcacagaa taaaggaaaa aagcggactt taccgtttta aagaaaaggg gaaaagaaat
    20281 ggaactaaac cgaatgatcg accatactat tttgaaagca gacgcatcta aagaagctgt
    20341 gatgcaaatt atcgaagaag caaaaaaata ccatttttat tctgtctgca tcaatccagc
    20401 atgggttgcg ctagcgaaag aacaattaca aggtgagcca gtagccgtat gtacagtaat
    20461 cgggtttcct ctaggagcaa acacacccga gacaaaagca tttgaaacaa cagatgccat
    20521 caataacggt gcagatgaag tcgatatggt catcaatatc ggtgctttga aatcaaagga
    20581 tgatcaacaa gtccaaaaag atatcgaagc agttgttgaa gcagcaaaag atcgtgcgct
    20641 tgtcaaagtt attattgaaa catcgttatt agatagagaa gaaatcatcc gagcttgcga
    20701 aatagcaaaa gctgcaggcg ctgattttgt caaaacatca actggtttct caacaggcgg
    20761 agcaaaggta gaggatgtcc gtttgatgcg tgaaactgta ggaccagaaa tgggtgtcaa
    20821 agcttccggc ggtatccata acgaagaaga agcaatggca atgatcgaag ctggtgctac
    20881 aagaatcgga acaagcgcag gagtagcaat cgtatctggt tcaactggtg aaggctatta
    20941 attttttcaa aggagaagct catggcagaa ctcaaacaag aatggatcaa cacagctatt
    21001 gaagcgttag ataaagccta tgttccttat tcccattttc cggtaggcgc ttgtctcgta
    21061 acggaatcag gcaagattta tcaaggcatc aacattgaga atgcatcatt tggattaacg
    21121 aattgtgccg agcgcacagc ttttttcaaa gcagtatctg aaggagaaaa aacatttacg
    21181 catctagtcg tagcggggca cacccccgat cctatctctc cttgtggtgc atgtcgacaa
    21241 gtaatggcag aattctgttc ccctgatatg ccggttacgc tagtaggaga caatggtgtg
    21301 acgaaggcaa cgaccgttcg tgcactgctg ccttatgcat ttactgaaaa agatctataa
    21361 ctcatgttca ttcagtcagc tgactgttga aaaataaaac aattatttta ggaggcttta
    21421 cagaaatgaa aaaagcaaaa ttatttggtt taggtgctgt cgcactagca gcagggttat
    21481 ttttaggtgc atgcggcaac aacggctcta ctgattcatc cggtggcaaa agctcatcag
    21541 acacaacaac tgcagcattg atcacagaca caggtggtgt agacgaccgt tcattcaacc
    21601 agtcagcttg ggaaggacta gaaaaatggg ggaaagatca aggactttca cgaggaaatg
    21661 atggcttcca atatttccaa tcttcaaatg aatcagatta catcccaaat attgaccaag
    21721 cattgaatgc tggattcaaa acgatttttg gtattggtta taaactaaaa ccagcgatcc
    21781 aagaacaagc aacaaacaat acaggtacga actttgttat tatcgatgat gtcatcgatg
    21841 gattagataa cgttgtatct gcaactttca aagacaacga agcttcttat cttgcaggtg
    21901 ttgcagcagc ttatacgact gaaacaaacg tagtcggctt tatcggcggt gtaaaaggtg
    21961 aagtaatcga tcgttttgat gctggattca aagccggtgt tgatgcaggt gcgaaagaat
    22021 taggaaaaga aatcaaagta ttgaatcaat atgctggtga cttctctgct ccagataaag
    22081 gacgttcaat tgctcaaggt atgtatgcac aaaacgcaga tatcatcttc catgcttcag
    22141 gcggtacagg taacggtgta ttccaagaag caaaatcatt gaacgaatca ggcgacaaaa
    22201 aagtttgggt tatcggtgtt gaccgcgacc aatcagacga aggcgaatac acattaaacg
    22261 gcgaaaagaa aaacttcaca ttgacatcta cactgaaagc agttggaaca gtagtagaag
    22321 atttggcaca aaaatcagcg gacggtaaat tccctggcgg cgaacacact gtttatggat
    22381 tgaaagaaga cggtgttggt ctaacagaag gacaactttc tgacgaagcg aaaaaagcag
    22441 ttgatgaagc aaaagaaaaa atcatttcag gtgacgtaaa agttccagaa actcctgaag
    22501 aaaactaaaa ccatttctat agccttgtta gacacatgtt tgacaaggct atggtttgat
    22561 tagaacaaga attgttcatt tatccaagct gccttgtgac agtttggata agggaacaac
    22621 tccctgaagt aatgtttgtt tcaaaagaag aacgctcact aagcgtgaat cttagtcaat
    22681 cgttgcccta gatagaaatc gttaggatgt gaaaaaaagg tggttcaaga aaatattgtc
    22741 atagaaatgc agaatattac caaagaattt ggaacattca aggcaaatga caacatcaat
    22801 ttacaagtaa aggcaggaga aattcatgcg ttgcttggag aaaacggtgc tggcaaatct
    22861 acattgatga acgtgctttc cggattatta gagccgacat cagggaaaat tttgatgcgt
    22921 gggaaagaag taaagatcac aagtccgacc aaagccaacc aattagggat tgggatggtc
    22981 catcagcact ttatgcttgt tgatgccttt actgtaacag aaaacatcgt gttgggaagc
    23041 gagcctagtc gcgcagggat gcttgaccat aaaaaagcac gaaaagagat ccaaaaagtt
    23101 tctgaacagt atggattgtc agtcaaccca gatgcttacg ttcgtgatat ttcagttgga
    23161 atggaacaac gggtagaaat tttaaaaaca ctttaccgag gagcagatgt actgattttt
    23221 gatgagccga cagctgtatt gacccctcaa gaaattgatg aattaatcgt gatcatgaag
    23281 gaattagtca aagaaggcaa gtcgatcatt ttgattacgc ataagttaga tgaaatcaaa
    23341 gcagtagctg accgctgtac agttatccgc cgtggaaaag gaatcggtac agtcaacgtt
    23401 aaagacgtaa cctcacaaca attagccgat atgatggttg gaagaacggt ttcattcaaa
    23461 acgatgaaaa aagaagcgaa gcctcaagaa gtcgttttgt ctattgaaaa tctagtggtg
    23521 aaagaaaatc gtggattaga agccgtgaaa aacttgaact tagaggttcg tgctggcgaa
    23581 gtacttggta tcgctggaat cgatggaaac gggcagtcgg agttgatcca agctttgact
    23641 ggtttgcgaa aggcagaaag cggacatatc aagctaaaag gggaagacat cactaataaa
    23701 aaaccccgaa aaatcactga acatggtgta ggacacgtgc cagaagaccg tcataaatac
    23761 gggttagtcc tagatatgac attatctgaa aatatcgcac tacaaaccta tcatcaaaaa
    23821 ccttacagta aaaacggcat gctgaattat tcagtaatga atgaacatgc cagagaattg
    23881 atcgaagaat atgatgttcg aacaacgaat gaacttgttc ccgcaaaagc tttatcaggc
    23941 ggaaatcagc aaaaagcaat tatcgctcgt gaagtcgacc gagatcctga tctgttgatt
    24001 gttgcaaatc caactcgtgg actggatgta ggtgcgatcg aatttattca taaacgtctg
    24061 atcgaacaaa gggacaaata caaagcagtg ttactgatca gtttcgaatt agaagaaatt
    24121 ttaaatgttt cggatcgtat tgctgttatc catgaaggag aaattgtcgg tatcgttgat
    24181 ccgaaagaaa catctgaaaa tgaattaggt ctattgatgg cgggctatac cttagaagaa
    24241 gcacgaaaag agttagaaaa gggggctggc gcacatgaat gatcgcagcg aaaaattccg
    24301 taatttatta gtgcctgtac tttcagttgt tttaggattg atcttaggcg cgatattgat
    24361 ggctgctttt ggttttcagc ccatccaagg ttattcttct atgctagatg cagcccttgg
    24421 ttcgcaaaga gggataggtg aaacgttaag gcaagcaacg ccgttgattt ttacagcatt
    24481 aggtttttct gtagctaact cagctgggtt tttcaatatt gggctttctg gacaagcact
    24541 ttgcgggtgg atcgtcagtg tctgggtggc acttgcgttt ccagatctgc caaaagttgt
    24601 tttgcttccg ttatgcgtga tcttaggtgc cttagctggt gcgatggcag cggctgttcc
    24661 aggtttactt cgagcatttt ttggaacaag tgaagtgatc gtaacaatca tgatgaacta
    24721 tatcttatta tacgtaagca catttgcttt acatgaagtg atgcctgaaa gctatcgctc
    24781 aagtttggac tcttccaata tgatcagtca aaatgcctca ctgcggttac catggttaac
    24841 acaagctttt ggcggctctc gcgtaaactc aggattattt ttagcaatca tcgcgcttat
    24901 tttcgtttgg ctgctgatga agaaaacgac tttaggattt gaaatccgtt ctgttggatt
    24961 gaatcctttt gcttctgagt atgcaggaat gagtagtaaa cgtacgatcg ttttgtcgat
    25021 ggtcatttct ggcgctttgg cagggcttgg cggtgtggtt gaaggattag gaacgtatca
    25081 aaacttcttt gttcaaacga cttcgctatc tattggattt gacggaatgg ctgtttcctt
    25141 gcttggttct ggatcagcaa tcggtattct tttatcagcg atcttgttca gtattttgaa
    25201 gatcggtgga cttggcatgc agacaggagc aggtgttccg tttgagatcg tcaatgtatc
    25261 gattgctttg attatcttct ttgtcgggat caacttcctg attcgtttcg tcatggctaa
    25321 agtcttccaa gggaaaaaac aagaagagat cgttgcgaca atagaatcga aaccaacgaa
    25381 tcaaaaccat gaagggggcg aactataatg tcgactatcg aattaattgc cttgatcctt
    25441 acttctacac tggtttattc aacaccgcta attctaacgt cactaggcgg aactttttct
    25501 gaacgaagcg ggatcgtaaa cgtagggcta gagggaatca tggtcatggg tgcttttagt
    25561 tccatcgttt tcaacttgac gatgagtcca tcacttggtt ctatgacacc atggatcgct
    25621 attttagtgg gtggcgtcgt aggtatccta ttttctctga ttcacgcagt agcaacagtc
    25681 agtttgcgtg ctgaccatat tatcagcggg acagtaatta atctgatggc acctgcttta
    25741 ggtgttttct tgatcaaagc atggtatggt aaaggtcaaa ctgataacat cacgcagaat
    25801 ctggggtatt tttctttccc aggattagca gatattccgg tcattggacc aattttcttt
    25861 aaaaatacat tcttacctgc ttattgtgct attttgattg ctgtcctagc ttgggtgatc
    25921 atcttcaaga cgaaattcgg cttgcgcctc cgttcagttg gtgaaaatcc tcaagctgcc
    25981 gacacattag gtatcaatgt atatgctatg cgttattcag gcgtactgat ttctggcttg
    26041 ttaggcggta tgggcggtgc tgtttttgct cagtcgattt ccggtaactt ttcagctggt
    26101 acgatcgttg gacaagggtt catctccatg gctgctatga tctttggtaa gtggaatcct
    26161 ttaggggcaa tgggtgcctc attgttcttt ggttttgctc aaagcttgag tatcattggc
    26221 gcacaattac cagttatttc ttcgattccg ccagtattgc tgcaaattgc tccatatttg
    26281 ctgacaatca ttgtgctggt tgttttctta gggaaagctt ctggtccaaa agcaaacggg
    26341 aagaactata tcaaatcaaa ataatctttt agcgaataaa aagggtgggt tacgggaagt
    26401 gtttttcgtc tcgccctttt tgttttaaag gcaataaaaa taaaaagaaa gaaggaataa
    26461 tcatgtttaa acgcgtacat ctgatcgtca tggattcagt aggaattgga gaagcaccag
    26521 atgccgataa gttcggtgat gttggttctg atacattagg acacattgcg aaagaagcag
    26581 gattgaacgt tccacattta gagcagcttg gtttaggaac gatccgtcct cttgaaggtg
    26641 tggaatcagt gaaagatcat caaggctatg cgacgaaatt agaagaagtt tctgtaggga
    26701 aagatacgat gacaggccac tgggaaatca tgggattgaa tatcaaaact ccatttcgtg
    26761 tgtttcctaa tggattccct gatgatctga tcaaacaaat cgaagaattt tctggccgta
    26821 aagtcgtatg taacaaacct tatagcggaa cagctgttat tgacgattac ggtgaacacc
    26881 aaatgaaaac aggcgattta attgtatata cttctgccga tcctgtcctt cagatcgcgg
    26941 cacatgaaga aatcattcct ttagaagaat tatatcggat ttgccaattc gttcgtgata
    27001 taacaaaaga tgatccgtat atgattggcc gaatcattgc acgtccgtac gtgggtgaac
    27061 caggagactt cacacgaaca agcaatcgcc atgattatgc gttgaatcca ttcggacaca
    27121 cagtccttga ttctttgaaa gaagcaggaa aggacgtgat tgctgttgga aagatcaacg
    27181 atattttcaa cggacaagga atcactgaat ctgtccgcac gaaaagcaat atggatggcg
    27241 ttgaccagct gctgaaagtt atgaaaaacg atttttcagg aatcagcttt accaatttag
    27301 tagattttga tgcattatac ggtcatcgtc gggatgtagt cggctatgcg catgcgattg
    27361 aagaatttga cttacgaatt ccagaattgc ttgaagcgat ggaagaagac gacttgctaa
    27421 tgattacagc cgatcacgga aacgatccga cgtttactgg taccgatcat acgcgagaat
    27481 atgtaccgtt attggtttac agtaaaaaaa tgaaagaaca aggggcattg cctcaaggat
    27541 attattcaga tatttcagca acgattgctg agaactttag tgtttcttcg actgaaaatg
    27601 gcgaaagctt cttgaaatta ttaaaataat ctttttaaat aggagaaagc aaatgaccaa
    27661 gttaagcgag atgttacaag aaacgaccca gtttatcctt gataaaggag taaaagaaat
    27721 tgattttggt ttgattctag gttcaggatt aggtgaatta gcagatgaaa tcgaagatgc
    27781 tgtaatgatt tcttatgatg agatcccgtt ctttcctgtt tcgacagtag ttgggcatgc
    27841 aggaaagcta gtttatgggt ctctggcagg caagaaagtg ttagccatgc aagggagatt
    27901 ccactactat gaaggtcatt ccatgcaaac agtgacctat ccagtacgtg tgatggccgg
    27961 actaggggcc cattcagtca ttgtgactaa tgcatgtggt ggtgtcaacg aatcatttgt
    28021 accaggagat ttgatgctga ttaccgatca tatcaatttt actggtgaaa atcctttgat
    28081 cggtccgaat gaagatgcaa tgggccctag atttccagat atgagtcaag cttatacgcc
    28141 tgactatcaa gcaacagcaa aagcagcagc agaaaaacta ggtttggatc ttaaagaagg
    28201 cgtatatatg ggctattctg gaccaactta tgaaacgcct gcggagatcc gaatgtctcg
    28261 gacaatagga gccgatgcag tagggatgtc gactgttcca gaagtcatcg tagcagctca
    28321 cagcggatta aaagtgttag gaatctcttg tattaccaac ttagcagcag gtatgcaagc
    28381 gaatctgagc catgaagaag ttgtagaaac aactcagaga gtcaaacaat cttttaaagc
    28441 attggttaaa gaaacattga cattattgta ataggaggaa caataaaatg agtgtgcata
    28501 ttgaagcgaa agaaggcgag atcgccgata agattttact tccgggagat cctttgcgag
    28561 ctaaatatat tgcggagact tttttggaag atccagtttg ttacaatcag gtacgcggta
    28621 tgttaggctt taccggcaaa tataaaggag aacgtatttc tgttcaagga acaggaatgg
    28681 gaatgccatc agccaccatc tatgcccatg aattgatcca gtcgtatggt gtaaagaaat
    28741 tgattcgagt aggtacatgc ggtgcattgt caaaagacgt ccatgttcgt gatttagtac
    28801 tagcacaagg tgcagcgacc agttcttcga tgatcgaaaa aaatttccaa gcctttcatt
    28861 ttcctccaat cagtgatttc aatctattac tgaaagcata tgagatagct aaagaaaagg
    28921 gctatacggt tcatgtagga aatgttcttt cggaagattc tttctacaaa gatgatttga
    28981 cagagacgtt ccaattagct gaacttggcg ttttaggagt ggaaatggaa gcagcggctc
    29041 tttattactt aggcgcgaaa taccatgtgc agacattaag tttgatgacc gttagcgacc
    29101 atttgatcac cggtgaagaa acaacagctg cagaacgtca atctacgttc aatgaaatga
    29161 tcgaagtagg attggaaaca gcgatagcag aataagaagg aaatgaaaaa gctgccctgc
    29221 attaagtaat aagaacatcc aagcaaaaat agctcctcat gtttttgact tggctggact
    29281 tattattgca aatgtaagct tttgaacacc gtttaccaag aggttgtgaa tctgccgttt
    29341 aactctgagt cacaaggaac aatttcaaaa aacagctttt catgtttgat gaatattgtg
    29401 acttgtgacc gagaagttgg caggagaaaa ccgtttatac gaaaaaaggg actgtgacaa
    29461 atcgtgtcac agtccctttt ttatagtgtt ttttataggt agtatttttt agcaactgtt
    29521 aagtcatcat ttaattcata aacaagtggt tgacctgttg gaatttcaag atccatgatg
    29581 tcttcgtcag agataccttc gatatgttta gctagggcgc gtaatgagtt accatgtgca
    29641 gctactaaaa cagttttgtt gtctagtaat gctggagcaa tttcatcttg ccagaatggc
    29701 aaagcgcgtt ctaatgtcac tttcaagttt tctccaccag gaatatcgcg tttgtctaac
    29761 attgcgtaac gacggtcatt tgctgcagaa ccttcgtcag tttcttccat taatggagga
    29821 agagtatcgt aagaacgacg ccagatgtgc acttgttcgt cgccgtattt ttcagctgtt
    29881 tcttttttgt tcaaaccttg taatttacca tagtgacgtt cgtttaagcg ccatgatttg
    29941 atttgtggta cccaaagttg atctgaatat tctaagatca agttacatgt tttgattgca
    30001 cgagttaaaa cagaagtgta agcaacatca aattcgattc cagcttcttt gattttgcgt
    30061 ccgccttctt ttgcttcttc gattccttct ggtgctagat ttacatcagc ccaaccagta
    30121 aattggttca atgcattcca ttcactaaga ccgtgacgag aaaaaactaa ttttggcatg
    30181 tttagctctc cttttaagat aggtttatat ttctgaaatt ttcagttctg ttttattgta
    30241 caattttttg gaagagattt ccatcgaaaa attagataag attctatgag aaaacaagta
    30301 gagaatataa aaaaacgaac agtgcccacg caactgttcg tttttaggta ttagatgcat
    30361 aattgctcga tttctttttc aggtaaattg atatatttat aacatgttcc aactgaaaca
    30421 ccgcattttg aagaaataaa ttggatcgtt tctttttttt cataatagag acgtcggatt
    30481 tttttgatcg tttttgcatc gatttttggt cttccgccga tttttccttt tttacgagct
    30541 tcatctaatc caactaatgt acgttctttg atcaatgcgc actccatgtt tgctaggttt
    30601 tccattaatt ggaaatagat ttttcccatt tcgcgcgtat cgattccttc atcaagacta
    30661 atcaagtgca gcccgcgatc tttgaataat tgcgtgagtt ctgttaattg ccgcgtagat
    30721 ttgcctaatc gatgaagttg gcaaatgact agtgcatcgc cttgttgcat gtcagcgatc
    30781 actgactcca aatgaacagc agtagaatca ttttgttcca cttcatatgt ctcatggaaa
    30841 atttcttgac aaccaaagtc agataattga tcaacctgag ttgttaaatc aacgtcattt
    30901 atggttgtgc gtgcataacc aataattttc atggtgattc ctttctagca gtgattttct
    30961 aactggatat aacagtaaaa atgtagcata aagtacgtca aaaagatagt tttttcgcaa
    31021 attttccgct gtcttttttt attggatgat caagcagttt ttctttattt acgacggatt
    31081 gcgtcaagaa tctcaacgcc tttttctgag ccgacaacta ctttagaaac catgtttagg
    31141 aaaagcccat gttctactac cccaacaagt tcatctaaat aggtagctaa gctttctggc
    31201 tgttcgatga cttctagatg cagatcaatg atgtaatgac caccatctgt ggttaagact
    31261 tctccgtttt cgtctgtacg caagacgggt tggaatcttt tttcttcaaa aatatgaagt
    31321 agctgttggg agccataagg gatcacttca actgggagtg ggaacttccc aagtttatcg
    31381 actaatttag aagaatcaac gatccaaatc gtttcttttg agtacgtagc gactattttc
    31441 tcgaaaagca aagcagcgcc gccgcctttg atcccttgaa aatcagcact gatttcatca
    31501 gcaccgtcta ttgtcaaatc gactacgggg acttcatcaa tactctttaa cggtattcct
    31561 aatttttccg cttgttcttt tgtcgcattt gaagtagtaa ctcctgtgat acgtaaacct
    31621 tcttcacgca tacggcgacc aatctcttct accatataat aggcggttga accggtcccg
    31681 agacctacga tcatcccgtc ctttacgaat ttcgcggctt caatgccagc taattgtttt
    31741 aaattcatct ttgcgtccac ctttttttag tttgagcatc gtgctttcct cactatttta
    31801 gcgtgattta cggtaaaaag ataccattct tttttattcc aataaagctt tttcaaaaaa
    31861 aattgcatct cttgttgaca tgttttttca ttcgtgatat tctaacaaag gtgctttatc
    31921 tacaggtttc tgtgaagaaa cgtgctgact gtttatcaaa gcatatttga taaggatgca
    31981 gacattgcat ttatttttta tcacaaaaaa agaaccacct ggatgtgtgg aactagacgc
    32041 aaatcgtggg aaggaggaaa caaggaatgc ctacaattaa ccaattagta cgtaaacctc
    32101 gtaaatcaaa ggtggaaaaa tctaattcac ctgctttgaa caaaggatat aacagcttta
    32161 agaaaactca aactaacgta aactcacctc aaaaacgtgg ggtagcaact cgtgtgggta
    32221 caatgacacc taaaaaaccg aactcagctt tacgtaaata tgcacgtgtt cgtttgtcaa
    32281 acttaattga agttactgct tatatcccag ggattggtca taacttacaa gaacacagtg
    32341 ttgtattatt acgcggtggt cgtgtaaaag acttaccagg ggtacgttac catatcgttc
    32401 gcggtgcttt agatactgct ggtgttaacg accgtaaaca aagccgttct aaatacggaa
    32461 ctaaaaaacc taaagcttaa ttttaattat taaacaaatg aaacttgctt ctactatata
    32521 gaagtaatta ctatgaacaa tatttcggaa ggaggagtta cggatgccac gtaaaggtcc
    32581 tgttgcaaaa cgtgatgttt taccagatcc tatttataac tcaaaattag taactcgctt
    32641 gatcaaccgt gtaatggttg acggaaaacg cggtattgca gctaacatta tctataatgc
    32701 ctttgacttg atcaaagaat ctacaggtaa cgatccatta gaagtgtttg aacaagcaat
    32761 gaaaaacgtt caacctgttc ttgaagttaa agcacgtcgt gttgggggtt caaactacca
    32821 agtaccagtt gaagttcgcc ctgaacgccg cgttactcta ggtcttcgtt gggtagtaaa
    32881 ctacgctcgc ttgcgcggtg aacacacaat ggaagaacgc ttagcaaaag aaatcatgga
    32941 tgctgcaaac aacacaggtg cttctgttaa aaaacgtgaa gacactcata aaatggctga
    33001 tgccaaccgt gcgtttgctc actatcgctg gtaagattct ctctgtttgg tcggctttta
    33061 taagtcgacc tcagatagtg tccttatttt tagcaactag atagtaaaga gaggagaaac
    33121 aaggagaatg accagagaat tttctttaca aaacactcgt aatatcggta tcatggccca
    33181 catcgatgct ggtaaaacga ctgctactga acgtatcttg tactatactg gtaaaatcca
    33241 taaaattggt gaaactcatg aaggagcttc acaaatggac tggatggaac aagaacaaga
    33301 acgtggtatt acgatcactt ctgctgctac tacagcacaa tggaagggtc accgaatcaa
    33361 catcatcgac acaccaggac acgtagactt cacagttgaa gttgaacgtt cattgcgtgt
    33421 acttgatggt gctgtaactg ttcttgatgc acaatcaggt gttgaacctc aaactgaaac
    33481 agtttggcgt caagcaacaa cttatggtgt acctcgtatc gtatttgcaa acaaaatgga
    33541 caagatcggt gctgacttct tatattcagt atcaacttta catgaccgtt tgcaagcaaa
    33601 cgctcaccca atccaattac caattggtgc ggaagacgac ttcacaggaa ttatcgacct
    33661 tgtaacaatg aaggctgaaa tgtatacaaa cgatcttgga acagagatcg aagaaacaga
    33721 aattcctgaa gaataccgcg aattagctga agaatggcgc gaaaaattgg ttgaagctgt
    33781 agctgaaact gatgaagaac ttactttgaa atacttagaa ggtgaagaaa tcactgaagc
    33841 tgagttaaaa gaaggtatcc gccgcgcaac agttaacgta gaattctacc cagtactatg
    33901 tggttcagca tttaagaaca aaggggttca attactattg gacgctgttc ttgattactt
    33961 gccatcaccg ttagatatcc cagctatcaa agggattgac cctaaaactg atgaagaagt
    34021 agaacgtcct gcagacgaca gcgctccttt ctcagcttta gcatttaaag ttatgactga
    34081 cccattcgta ggtcgtttaa ctttcttccg tgtgtactct ggtgttttac aatcaggttc
    34141 atacgtacaa aacgcaacaa aaggcaaacg tgaacgtgtt ggtcgtatcc tacagatgca
    34201 tgccaactct cgttcagaaa tctctgaagt ttacgctgga gatatcgctg ctgctgttgg
    34261 tttgaaagac acaactactg gggacacact atgtgatgaa aagagtctag ttattcttga
    34321 atccatggaa ttcccagaac cagttatcca agtcgctatt gaacctaaat caaaagctga
    34381 ccaagataaa atgggtgtgg ctttacaaaa actatctgaa gaagatccta ctttccgtgc
    34441 tgaaacaaac gtggaaacag gcgaaacaat catcgctggt atgggtgaac ttcacttaga
    34501 tatcatcgtt gaccgtatgc gtcgtgaatt caaagttgaa gctaacgtag gtgcgcctca
    34561 agtatcttac cgtgaaactt tccgtgctgg cactcaagcc gaaggtaagt ttgtacgtca
    34621 gtctggtggt aaaggtcaat acggtcacgt atggattgaa tttacaccaa atgaagaagg
    34681 tgctggtttc gaatttgaaa atgcgatcgt cggtggggtt gttcctcgtg aatacattcc
    34741 agctgttgaa actggtttga aagatgctat ggaaaatggt gtcttagctg gctatccatt
    34801 agtagatatc aaagctaaac tttacgatgg ttcataccat gatgtcgatt caaatgaaac
    34861 tgccttccgt gttgctgctt ctatggcatt acgtgcagca gctaagaaag ctaatccagt
    34921 aatccttgaa ccaatcatgg ctgtagaagt tgttatccct gaagattatt taggtgatgt
    34981 tatgggtcac gtaactgctc gtcgtggacg tgttgaaggt atggaagctc gcgctggcgg
    35041 ccaacaagtt gttcgtgcga tggttccact tgctgaaatg tttggttatg ctacaactct
    35101 tcgttctgct actcaaggtc gtggtacatt tacaatgaca tttgaccact acgaagatgt
    35161 accaaaatca gtacaagaag aaatcattaa gaaaaacggc ggaaaagctg aataatttct
    35221 tgaaaccgga tagtcctgca aagagattat ccaaagagac tatattttta atatagtttc
    35281 gtgtaaaata attatcgatg atatgggcat gtggtgtacc ttgtgtacat catataccta
    35341 tcagacaaat aaaaaatata ttgtattttt aggaggacat tttaaaatgg caaaagaaaa
    35401 atttgaccgt tctaaaccac acgttaacat tggtactatc ggacacgttg accatggtaa
    35461 aactacatta acagctgcaa tcacaactgt actatctaag aaaaacggcg gccaagctat
    35521 ggcttacgat caaatcgatg gtgctccaga agaacgcgaa cgcggaatca caatctcaac
    35581 agctcacgtt gagtatgaaa cagatacacg ccactacgct cacgttgact gcccaggaca
    35641 cgcggattac gttaaaaaca tgatcactgg tgctgctcaa atggacggag ctatcttggt
    35701 agtttctgct gctgacggcc caatgcctca aactcgtgaa cacatcctat tgtctcgtca
    35761 agttggtgtt ccttacatcg ttgtattctt gaacaaagta gacatggttg atgacgaaga
    35821 attactagaa ttagttgaaa tggaagttcg tgacctatta acagaatacg aattccctgg
    35881 tgacgatgtt cctgtagttg ctggatcagc tttgaaagct ctagaaggcg acgcttcata
    35941 cgaagaaaaa attcttgaat taatggctgc agttgacgaa tacatcccaa ctccagaacg
    36001 tgacaacgac aaaccattca tgatgccagt tgaagacgtg ttctcaatta ctggacgtgg
    36061 tactgttgct acaggtcgtg ttgaacgtgg acaagttcgc gttggtgacg aagttgaagt
    36121 tgttggtatt gctgaagaaa cttcaaaaac aacagttact ggtgttgaaa tgttccgtaa
    36181 attgttagac tacgctgaag ctggagacaa cattggtgct ttactacgtg gtgttgcacg
    36241 tgaagacatc caacgtggac aagttttagc taaaccaggt acaatcacac ctcatacaaa
    36301 attctctgca gaagtatacg tgttgacaaa agaagaaggt ggacgtcata ctccattctt
    36361 cactaactac cgtccacaat tctacttccg tacaactgac gtaacaggtg ttgttgaatt
    36421 accagaagga actgaaatgg ttatgcctgg cgacaacgta acaatggaag ttgaattaat
    36481 ccacccaatc gctatcgaaa acggtactaa attctcaatc cgtgaaggcg gacgtacagt
    36541 tggtgccggt gtcgttacag aaatcaaagc ttaattcact taagttttaa agtaaagcaa
    36601 catttattcg gacgtaatcg ctcggaacga aggtcttagg atctttgttc cgagcttttt
    36661 tatttattgc aggatcagga aaaaagacag gagtattata aatagaagaa acacgaaata
    36721 aaagagtcag tattttctaa atgaaacttt atgaaaacaa aatgtttttt acaagaaaaa
    36781 gccttgcttt gtgagtttat atatattata atgacaaagg tgcgtttact ttagtgaatt
    36841 gcacagacgt aatcagaatg atttcgtcgg ctaagaaacg agaggttgcg acacgcccgg
    36901 acgctttgcc acgaacgagc gtgacggaaa ttttcgtgga gctatgtcta cttttcaaaa
    36961 taggcgaagg agggaacaag atggcaaaac aaaaaattcg tatccgttta aaagcgtatg
    37021 aacaccgtat tttagatcaa tcagcggata aaatcgtgga aactgcaaaa agaactggag
    37081 ctagcgtgtc tggtccaatt ccattaccaa ctgaacgcag tctttacaca atcatccgtg
    37141 cgactcataa atacaaagat tcacgcgaac aattcgaaat gcgtacacac aaacgtctta
    37201 tcgacattgt gaacccaaca ccaaaaactg ttgatgcttt gatgaagcta gacttaccat
    37261 ctggtgtaaa catcgaaatc aaattataaa actaaaacaa aaataatgga ggtgtactca
    37321 tgaccaaagg aatcttaggg aaaaaagtgg gaatgacaca aatcttcact gaatctggtg
    37381 aattaattcc agtaactgtt gtagaagcta ctccaaacgt agtactacaa gtgaaaacaa
    37441 tggaaacgga tggctacgaa gctattcaag ttggttacca agatatgcgt gaagttttat
    37501 caaacaaacc tgcgaaaggt catgttgcaa aagcaaacac ggctcctaag cgcttcattc
    37561 gtgaattcaa aaatgttgag ctaggagaat atgaagtagg aaaagaaatt acagtagatg
    37621 ttttccaagc aggagacatt attgatgtaa caggtactac gaaaggtaaa ggattccaag
    37681 gggttatcaa acgccacgga caaagccgcg gaccaatggc tcacggttct cgttaccatc
    37741 gtcgtcctgg gtcaatgggt ccagttgcac ctaaccgtgt atttaaaaac aaacgtcttg
    37801 ctggccgtat gggtggcaac cgcgtaacta tccaaaactt ggaagttgta cgtgtcgatg
    37861 cagaaagaaa tgttatcttg atcaaaggta atgttcctgg agctaaaaaa tctttaatta
    37921 ctatcaaatc agctgtgaaa gctaaataat tgtaggaaga aaggaggaac taaggaatgc
    37981 cgaatgtagc attattcaaa caagatggaa gccaaaacgg tgaaatcaca cttaacgaag
    38041 aaatcttcgg aattgaacct aatgaaagtg ttgtttacga tgcaatcgta atgcaacgtg
    38101 cgtcattaag acaaggaaca cacgcggtta aaaaccgtag cgctgttcgc ggcggcggac
    38161 gtaaaccatg gcgtcaaaaa ggaacaggtc gtgcacgtca aggatctatc cgctcaccac
    38221 aatggcgtgg aggtggcgta gtctttggac caacaccacg ttcttacagc tacaaacttc
    38281 ctaaaaaagt tcgtcgttta gcaatgaaat ctgtattgtc agaaaaagtt gctgaaaaca
    38341 acttggtagc aatcgaaggc ttgaacttcg acgcaccaaa aacaaaagaa ttcaaacaag
    38401 ttcttgctaa cttatctatc gactctaaag tattggttgt tctagaaaaa ggtaacgact
    38461 ttgctgcttt atcagcacgt aacttaccaa acgtttctgt agtaacttct gataatgtga
    38521 gtgtgctaga cgttgtatca aatacaaaag tgttagcaac acaaactgct cttactcaaa
    38581 ttgaggaggt gcttgcataa tgaacttact agacgtaatc aaacgcccag tgatcactga
    38641 aaaatcaatg cttgccatgg atgacaagaa atacactttt gaagtggaca ctcgcgcaaa
    38701 caaaacttta gtaaaacaag cggtagaagc tgcttttgac gttaaagtca aaaacgtgaa
    38761 catcgtgaac gtacgtgcga aattcaaacg tatgggcaaa tatgcaggat atacaaaaaa
    38821 acgtcgcaaa gcgatcgtta cattgactga agattcaaaa gaaatccaat tattcgaagc
    38881 tgctgaataa gcactaatag ataactcatt aaattaggag ggaaaaacgt ggcgattaaa
    38941 aagtacaaac ctacctcaaa tggtcgtcgt aacatgacag gttctgattt tgctgaaatc
    39001 acaacatcaa cacctgaaaa atcattgtta caaccattga aaaaccatgc tggacgtaac
    39061 aacaacggcc gcatcactgt acgtcatcaa ggtggcggac acaaacgcca atatcgcgtg
    39121 atcgacttca aacgtaacaa agataacgtt gttgcaactg ttcaaacgat cgaatatgat
    39181 ccaaaccgtt cagctaacat cgcgttagta cactatgaag atggagtgaa agcttacatc
    39241 ttagcaccta aaggactaga agtgggtatg aaacttgttt caggtccaga agcagatatc
    39301 aaagtcggaa atgctcttcc attagaaaac attccagttg gtactgttat ccacaacatt
    39361 gaaatgaaac ctggtaaagg tggacaattg atccgttctg ctggtacaag tgctcaagta
    39421 cttggtaaag aaggcaaata cgtattgatc cgcttgaact ctggtgaagt tcgtatgatc
    39481 ttagctgcat gccgtgctac aatcggttct gtaggtaacg aacaacacga attaatcaac
    39541 atcggtaaag ctggacgctc tcgttggatg cgtaaacgcc caactgtacg tggtagcgta
    39601 atgaacccta atgatcaccc acacggtggt ggtgaaggta aagctcctat cggacgtaaa
    39661 gcaccagttt ctccttgggg tcaaccagct cttggttaca aaactcgtaa caaaaaagct
    39721 aaatcagaca aacttattgt tcgtcgtcgc aaaaataaat aattactttg accatgtgtc
    39781 gcacattttg agaggaggtt caccatgggt cgtagtttaa agaaaggacc ttttgtcgat
    39841 gagcatttaa tgaaaaaagt tgaagctcaa gcaggggttg aaaagaaaaa agttatcaaa
    39901 acttggtcac gccgttcaac agttttccca agttttgttg gatataccat cgcagtttat
    39961 gatggacgta aacatgtacc agtttacatc caagaagaca tggtaggaca caaattaggt
    40021 gaatttgcac ctacaagaac ttatcgtgga catgctgctg acgataagaa aacgaaacgc
    40081 taatttgagg ggaggataaa tccaaatggc agaacaaatc acatcagcta aagcaactgc
    40141 aaaaacagtt cgcacttcac ctcgaaaagc ccgtttagta attgacctta tcagaggtaa
    40201 aagtgtcgca gatgcaattt caatcttgaa attcacacca aacaaatctg ctggaatcat
    40261 tgaaaaagta ttgatgtcag cagttgcgaa tgcagaaaat aactttgact tagatgttga
    40321 aaacttggta gtaagcgaag cttttgttaa cgaaggacct acaatgaaac gtttccgtcc
    40381 tcgtgcaaaa ggatcagctt ctccaatcaa caaacgtaca agtcatatca cggtagtcgt
    40441 atcagaaaaa taaggaggga taatcagtgg gtcaaaaagt acatccaatt ggaatgcgtg
    40501 taggcatcat ccgcgactgg gatgctaaat ggtatgctga aaaagagtat gctgagttct
    40561 tacacgaaga tttaagaatc cgtaaattta tttcaactaa acttgctgat gctgctgtat
    40621 caacagttga aatcgaacgc gcaacaggtc gcgtaaacat ttcaattcac acagctaaac
    40681 caggtatggt tattggtaaa ggcggttctg aagtcgaaag cctaagaaaa gaattaaaca
    40741 aattaactgg taaaagaatc catatcaata tcgtggaaat caaaaaacca gatctagatg
    40801 caaaattagt aggtgaagga atcgcacgtc aattagaaaa ccgtgttgct ttccgtcgcg
    40861 ctcaaaaaca agccatccaa cgctcaatgc gtgctggcgc tcaagggatc aaaactcaag
    40921 tatctggtcg tctaaacgga gcagatatcg ctcgttcaga aggatactca gaaggaactg
    40981 ttcctcttca tactttacgt gcggacattg attacgcatg ggaagaagca gatacaacat
    41041 acggaaaact aggagttaaa gtgtggatct atcgtggaga aattcttcca actaaaaaaa
    41101 acactgagaa aggagggaaa taatcatgtt agtacctaaa cgtgtaaaac accgtcgtga
    41161 attccgcgga aaaatgcgcg gggaagctaa aggcggaaaa gaagtagcat tcggtgaata
    41221 cggtttgcaa gctgttgatt cacattggat cacaaaccgc caaatcgaag ctgctcgtat
    41281 cgcaatgact cgttacatga aacgtggtgg gaaagtatgg attaaaattt tccctcacaa
    41341 atcttatact gccaaagcaa ttggggtacg tatgggttct ggtaaagggg cacctgaagg
    41401 atgggttgca ccagtaaaac gtggtaaaat catgtttgaa atcgcaggcg ttcctgaaga
    41461 agtagctcgt gaagcgttac gtctagcttc tcacaaatta ccaatgaaaa ctaagatcgt
    41521 aaaacgtgag gaaatgggtg gtgaatcgaa tgaaggttaa agaaatcaga gaattaacca
    41581 ctgccgaaat gcttgatcaa gaaaaacaat taaaagaaga attgtttaat cttagattcc
    41641 aattagcaac aggtcaatta gaaaacactg cacgtattaa agaagtacgt aaatcgattg
    41701 cacgcatcaa aactgtgttg cgtgaacaag ctaagtaatt atggaaggag gccattaagc
    41761 gtatgactga aggaagaaat caacgcaaag tttatcaagg tcgcgtggtt tcagacaaaa
    41821 tggataaaac aattaccgtt gctgtcgaaa caaagaaaaa ccaccctatc tatggcaaac
    41881 gcatgaacta ttctaagaaa tacaaagtgc atgatgaaaa caacacagca aaagttggcg
    41941 acatcgtgag aattatggaa actcgtccat tatcagctac aaaacgtttc cgtttgttgg
    42001 aagtagtcga agaagcagtt attatctaat aacacgagat tttcctatat agattgaatg
    42061 caaaatctga aaggaggata cacaacgtga tccaagcaga aagtcgttta aaagtcgctg
    42121 ataactcagg cgcacgtgaa attttgacga tcaaagtcct tggtggatct ggtcgtaaaa
    42181 ctgctaacat cggtgatgtg atcgttgcta cggttaaaca agcaacgcca ggtggggttg
    42241 tcaaaaaagg tgacgtcgtt aaagccgtta tcgttcgaac aaaatcagga gctcgtcgta
    42301 cagacggttc atatattaaa tttgatgaaa atgctgcagt gattattcgt gatgataaaa
    42361 gccctcgtgg aacacgtatc tttgggccag ttgcacgtga actacgtgaa aacaacttca
    42421 tgaagatcgt ttcactagca ccagaagttt tataatcttg agacacttat caaaggaggt
    42481 gcgaaacagt tatgtttgtt aaaaaaggcg ataaagttaa agttatcact ggaaaagaca
    42541 aaaacaaaga aggcgttgta ctagcagcgt tcccgaaaaa agataaagtt attgtcgaag
    42601 gtgtaaatgt cgttaaaaaa caccaaaaac cttctcaagc tgctcctcaa ggtggaattg
    42661 ttgagatgga agcgccaatt catgtttcta acgtgatggt ggttgactct actggtgtag
    42721 ctggccgagt tggctacaaa gaagtagacg gcaaaaaagt ccgtgtttct aaaaaaaccg
    42781 gtgaagtttt agataaataa aagtttaagg aaggagggtc ttactgaatg aaccgcctaa
    42841 aagaaaaata tcttaaagaa gtaactccat cattgatgga aaaatttaac tatagctctg
    42901 ttatgcaaac acctaaagtt gaaaaaatcg tcatcaacat gggtgttggt gatgcggtat
    42961 caaacgctaa aaacttagat aaagcagttg aagaattaac tttgatcact ggtcaaaaac
    43021 caatgattac aaaagctaaa aaatcaatcg ctggattccg tttacgtgaa ggaatgccaa
    43081 ttggtgcaaa agttacattg cgcggagaaa gaatgtacga attcttagac aaattggttt
    43141 ctgtttcact accacgtgta cgtgacttcc atggtgtaag taaaaaagcc tttgacggtc
    43201 gtggaaacta tactttaggt attaaagaac aattgatctt cccagaagta gattatgatt
    43261 tagtagataa agtacgtggt atggacatcg ttattgtaac aacagcaaac tctgatgaag
    43321 aatctcgtga gttgttgaca caattaggca tgccattcca aaaataatta aaggaggcga
    43381 actacgtggc taaaaaatca atgattgcta aaaacaaacg tcctgcaaaa ttttcaacac
    43441 aagcttatac tcgttgcgaa cgttgcggac gtccacattc agtttatcgt aagttccatc
    43501 tttgccgtat ttgcttccgc gaacttgcct ataaaggtca aattcccggc gtgaagaaag
    43561 ctagctggta aaaagtaaat tcgcataaga aggaggtaaa agatcaatgg tcatgacaga
    43621 tccaattgca gattttctaa ctcgtatccg taacgcaaac atggttaaac atgagtcttt
    43681 agaagtacct gcatcaaaaa ttaaacgtga catcgctgaa atcttgaaac gtgaaggttt
    43741 cgttcgtgat gttgaataca tcgaagatga caaacaaggc gtgatccgtg ttttcctaaa
    43801 atttggtaaa aacggcgaac gtgttatcac aaacttaaaa cgtatctcta aaccaggttt
    43861 gcgtgcttat gtcaaagctg acgaagtacc aaaagtattg aacggtttag gtatcgcaat
    43921 catttcgact tctgaaggtg ttgtcactga taaagaagct cgtgaaaaaa atatcggcgg
    43981 cgaagttatc gcttacgtat ggtaatcaag tagaatctac aaggaggtgt ctttaagtga
    44041 gccgtatcgg aaataaagtc gttgttatcc ctgaaggagt aacagttact caagacggaa
    44101 acaacgttac agttaaagga ccaaaaggtg aattgactcg tactttttca gcagatatca
    44161 aaatgaatat cgaaggaaac gaagtaactt tcactcgtcc aaacgacagt aaagaaatga
    44221 aaacaattca tggaacaact cgtgcgaact tcaacaacat ggttgtaggt gtcagcgaag
    44281 gtttccaaaa agcgttagaa ctaatcgggg ttgggtaccg tgcacaatta caaggatcaa
    44341 aacttgtatt gaacgttggt tactcacatc cagtagaaat cacaccacca gctggagtaa
    44401 ctgttgaagt accagctaac acacaagtaa ttgttaaagg tgcgaacaaa gaagaagttg
    44461 gcgaattagc tgcaaacatt cgtggaactc gtcctccaga accttataaa ggtaaaggta
    44521 ttcgttatgt tggcgaatat gtacgccgta aagaaggtaa aactggtaaa taatcgttta
    44581 gaaaaattgc atagaagcca gcagcaggaa aaaggcattt ggctgaagtt taccttcaaa
    44641 aaaatacatt tttacttgtg ccgcagcttc tatttttcga aacgtattta ctgcttgaaa
    44701 tagtagtaca ataaaaataa agaggtgaca attgtgatta caaaaccaga taagaacaaa
    44761 acacgtcaaa agagacatcg tcgtgtacgt aacaatatct ctggtactgc tgagcgccca
    44821 cgcttgaacg tttttcgttc taacaaaaac atctacgctc aaattattga tgacgtagcg
    44881 ggtgtaacgc tagcaagtgc ctctaccttg gataaagaaa tttcaggtgg aacaaaaaca
    44941 gaagcagcac aagctgtcgg taaactagtt gctgaacgtg cagctgaaaa aggcattaaa
    45001 gtagtagtct ttgaccgtgg tggatacctt taccatggcc gtgtgcaagc tttagctgaa
    45061 gctgctcgcg aaaatggact agaattttag aagaaggagg aataccattc atggtttata
    45121 tcgatccaaa acatttggaa ttagaagacc gcgttgttgc aattaaccgt gtaacaaaag
    45181 ttgttaaagg tggacgtcgt ctacgttttg ctgctttagt tgttgtcggt gacaaaaacg
    45241 gacacgtagg gtttggtaca ggtaaagctc aagaagttcc tgaagcaatc cgtaaagcaa
    45301 ttgaagatgc gaagaaaaac ttagttgaag tacctatggt tggttcaaca atcccacacg
    45361 aagtaatcgg tgcttttagt ggtggtagaa tcctaatgaa acctgctgta gaaggttctg
    45421 gggttgctgc tggtggacca gttcgtgccg tattagaatt agctggggta gcagatatca
    45481 cttctaaatc tttaggctca aatacaccaa tcaacgttgt tcgcgcaaca gttgaaggtt
    45541 tgaaacaatt gaaacgtgca gaagaagttg cagaacttcg cggcaaatca gttgaagaat
    45601 taatcggata aggaggacaa caataatggc tgaattaaaa gttactttaa aacgcagtat
    45661 tatcggacgt cctcaaaacc aacgcgatac agttaaagcg ttaggtctta caaagattaa
    45721 cagttcagtg gttaagcctt ctaatgaagc aattaaaggc atgatcaata ctgtttctca
    45781 cctagtggac gttgaagaaa tttaatttag aaaaatcata actagaacat taaggaggtg
    45841 ccaaacctat gaaacttcat gaattaaaac ctgctgaagg ttcacgccaa gtacgtaacc
    45901 gtgttggtcg tggtacttca tctggtaacg gaaaaactgc cggacgcgga caaaaaggac
    45961 aaaaagctcg ttcaggcggc ggtgtgcgtt taggatttga agggggacaa acaccattgt
    46021 tccgtcgttt accaaaacga ggattcacaa acgtgaaccg taaagactat gcagtcatta
    46081 atcttgatgt attgaaccgc tttgaagacg gagctgaagt aactcctgtt gctttagttg
    46141 aagctggaat cgtgaaaaac gaaaaagctg gaatcaaagt attagctaat ggggaactaa
    46201 acaagaaatt aactgtgaaa gcagcaaaat tctctaaagc agctcaagaa gcaattgaag
    46261 cggctggtgg atcaatcgag gtgatctaat gtttagacta ttaaaagatg cttttaaagt
    46321 aaaagacatt agatcgaaaa tcctatttac tgtatttgtg ttgtttgtat ttcgcctagg
    46381 tgcacatatt actgtaccag gcgtaaatgc tgcttcattg caaaaccttt cttctttgcc
    46441 atttttcaac atgttgaact tggtaagtgg tagtgcgatg caaaactttt ccattttctc
    46501 aatgggggtt tcaccataca ttactgcttc tatcgtcatt cagcttcttc aaatggacat
    46561 cgtgcctcga tttgttgaat ggtcgaaaca aggggaagtt ggtcgtaaaa aactgaatca
    46621 agcgactcga tatctgacat tagtattggc atttatccaa tctgtcggta tcactgctgg
    46681 attcaatcaa tacgctcaat taggtttcgt gaataatcct aacgtagcaa cttacgtaac
    46741 gatcggtttg attctaacag ccggtacgat gtttgttact tggctaggag aacagatcac
    46801 agaaaaaggt atcggaaatg gcgtatcaat gatcattttt gcaggtatca tttctagatt
    46861 gcctgaaagt atcagagaaa tttatgaaga ttactttata aatattgatc cttcagacat
    46921 atggaaatct gctatttttg tggccatttt actagtagcg gtcttgttga tcgtgacatt
    46981 cgtaacattt ttccaacaag ctgaacgcaa gatcccgatc caatatacga aacgcgtagc
    47041 tggcgcgcct acaagcagct atctgccgtt gaaagtaaat gcagctggag ttattccagt
    47101 tatctttgct agttcgttta ttgctacgcc aaatgctgtt ttacaagcat tgagcggtag
    47161 attcggttct gaaaactggt acaaagtgat gatggagatc tttaactata atacagcacc
    47221 tggtgctacg atttatacga ttctgatcgt tgccttcact ttcttctacg cctttgttca
    47281 agtaaaccct gagaaattag cggaaaactt gcaaaagcaa ggaagttata taccaagcgt
    47341 acgccctggt aaggggactg aagagtatgt ctcaagatta ctgatgcgtt taagtacagt
    47401 tggtgcagtc ttccttggtt tagtggcatt gcttccaatc attgctcaaa tggtttggga
    47461 tcttccacaa tcaatcggat taggcggtac aagcttactg atcgttatcg gtgtagcttt
    47521 agaaactgcg aaacagttag aaggcttaat gctgaaacgt aaatatgttg gattcattga
    47581 gtaagaaagc gtgttgaggt ttcccctcaa cacacgttct cacacattta ttaggaggga
    47641 aaacaatgaa cctcatttta atggggctgc caggcgcagg taaaggaaca caagctgaaa
    47701 aaatcattga tgcttatggt attccgcaca tctcaacagg agatatgttc cgcgcagcga
    47761 tgcaaaacga aacagctctt ggcctggaag caaagtctta tatggataaa ggtgcattag
    47821 ttcctgatga agtaacaaat ggaatcgtga aagagcgact agctgaacca gatacagaga
    47881 aaggcttctt attagatggc tttccacgta cgctagatca agcaaaagct ctggacgcaa
    47941 tgctaaaaga tttgaataaa aaaattgatg ctgtcatcga tatccatgtt ggtgaagaaa
    48001 tcttggtcga acgtctagcc ggccgattta tctgcagcaa ttgtggagca acttaccata
    48061 aaattttcaa ccctacaaaa gttgaagaca catgtgatcg ttgcggtggg catgaattct
    48121 atcaaagaga agatgataaa cctgagacgg ttaaaaatcg tctggctatc aacatcaaaa
    48181 atagtgaacc aatcttggct tattataaag aacaaggttt gctaaacact attgatggtg
    48241 ctcgtgaaat cgatgctgta tttgcagatg tacaaaaaat cattgaaaaa tagcacgatt
    48301 gcccgtagcc gacttgcatt atgcagttac tcttgtcaag ttcaataaat tatgatagaa
    48361 tatgtcagtc cgacttctag agaatcctct agatgatcca ttttgagaat tttgaggtgg
    48421 gaactgcagt ttccgccatt ttatcgtgtg aaaatgcgaa acaagtacaa ggaggtactg
    48481 tgcgtggcaa aagaagatat gattgaagtc gaaggtacag tcgtcgaaac tttgccgaat
    48541 gcaatgttta aagttgaact agaaaacgga caccaagttc ttgctactgt ttcaggtaaa
    48601 attcgtatgc attacatccg tatcttacct ggtgacaaag taacggttga attgtctcca
    48661 tacgatctta accgtggccg tattacttat cgctttaaat aattgtactc cgtaaaacta
    48721 caggaggtat tatcatgaaa gtaagaccat cagtaaaacc aatgtgcgaa cattgtaaag
    48781 taattcgtcg taaaggacgc gttatggtga tttgcccagc aaatccaaaa cataaacaac
    48841 gtcaaggata attggaggtg ctataaatat ggctcgtatt gcaggagtag atattcctcg
    48901 tgataaacgt gtagtagttt cacttactta tatctatggt attggtaaca ctactgctaa
    48961 aaagatctta gctgatgctg gtgtatctga agatgttcgc gttcgtgaat taacgaatga
    49021 acaaacagat gctatccgtg cagaagtaga taaattaaaa gttgaagggg atcttcgtcg
    49081 tgaagtgaac ttgaacatca aacgtttgat ggaaatcggt tcttaccgtg gaatccgtca
    49141 tcgccgtgga ttgcctgttc gtggacaaaa cacgaaaaac aatgcacgta ctcgtaaagg
    49201 cccagctcgc gctatcgctg gcaagaaaaa ataattctta agtgaaggag gttaaaactt
    49261 catggcagca aaaaaagtga atcgtaaacg ccgtgtgaag aagaatatag aagcaggtat
    49321 tgcacatatc cattctacat tcaataacac tatcatcatg atcactgata ctcacggaaa
    49381 tgcattagcg tggtcatcag ctggttcatt aggtttcaaa ggaagcaaaa aatcaactcc
    49441 ttttgccgct caaatggcag cagaaactgc tacaaaagca gcaatggaac acggattaaa
    49501 gactgttgaa gtaacagtta aaggacctgg ttctggacgt gaagcagcaa ttcgttcatt
    49561 acaagctaca ggtttagaag tgactgcaat tcgtgacgtg actccagttc ctcataatgg
    49621 tgcccgccct ccaaaacgcc gtcgtgttta atgagtgccg ctcattttga gttttatcag
    49681 taacgtcatt gaacaagaca cttttcgttt tgaaaggggt aaagataaga atgattgagt
    49741 tcgaaaaacc aagaatcgca aaaattgatg aagaaaaaga ttatggcaag ttcatcgttg
    49801 aacctcttga aagagggtat gggactactt tagggaattc cctacgtcgt attttattat
    49861 cttctttgcc aggtgctgcc atcactaata ttcaaatcga tggtgtactg catgaattct
    49921 ctaccatcaa aggtgttaga gaagacgtca ctcaaatcat cttgaacatt aaaggcttag
    49981 cgctgaaaat gtatggccaa gaagaaaaaa cccttgaaat cgatattacc ggacctgcta
    50041 cagtaactgc tggcgatatt atcgttgata gtgatgtaga aattcttaac aaagatatgt
    50101 acatctgtag tgtctctgaa ggagctacgt tccatgctcg cttaacagtg aaacctggac
    50161 gtggttatgt tcaagcagat gaaaataaaa aagaagatat gccaattggc gttcttccag
    50221 tcgattcaat ctacacacct gttcgtcgtg tgaactatca agttgagaac acacgtgttg
    50281 gacaccgtga agattttgat aaattgacga tggagatttg gacagatggt tcaattgaac
    50341 cgttggaagc aatgagtttg tctgcgaaaa tcatgactga acatttggat atttttgtga
    50401 acttaactga tgaagcaaaa aatgctgaaa tcatggtcga aaaagaagaa acacaaaaag
    50461 aaaaaatgtt ggaaatgacg atcgaagagt tagacttgtc tgttcgctct tacaattgtt
    50521 tgaaacgtgc aggaatcaat actgtacaag aacttacaaa taaatctgag cctgaaatga
    50581 tcaaagtccg taacttagga cgtaaatcat tagaagaagt taaagcaaaa ttgcacgact
    50641 taggtttagg tttacgtaaa gaagattaaa accttttacg aaggagggaa accaacgtga
    50701 gttatcgtaa actaggacgc acatctagcc aacgtaaagc gatgttgcgt gatttaacaa
    50761 ctgatttatt aatcaacgaa cgtattgtga ctactgaagc tcgtgcgaaa gaagttcgct
    50821 caactgcaga aaaaatgatc actttaggta aacgcggaga tttgcatgca cgtcgtcaag
    50881 ctgcggcttt cgtacgtaat gaagttgcca gcgtgcgtga agaaaatgag gacatcgtca
    50941 ttgaatcagc tttacaaaaa ctgttcaacg atattgcacc tcgttacgca gaacgtcaag
    51001 gcggatacac acgcattttg aagacagaac caagacgcgg tgatggtgca ccaatggttg
    51061 tcttagaact tgtctaaaat cacactaata acatcacttt atagatgatt cttttagagt
    51121 gttatgatgt tggaagatat ttttccgagt ctagctcagc gctgctcctc aagcgaatat
    51181 cgcttgggga gtaagcactc atcataaagt gtttttttat accttcaatt acctgtcatg
    51241 acagatatgt ttgaaggcat aaaaaaacat tttaagcgag aaataattga aaattttgtc
    51301 tttttttgat agtgttagta ggaactttag gatgaagaag gagtaatcat ggagccaatt
    51361 attgaattag aaaagataaa ttataaatac cagccagatg atcttcgccc cgcattgaaa
    51421 gatgtctcct ttacaattga taaaggtgag tggatcgcta ttattggaca caatggttca
    51481 ggaaaatcga cacttgccaa aacgatcaat ggattgcttc tgccagaatc agggatcgtg
    51541 aaagtgggaa atcaaatatt ggatgaagaa aatatttgga cgatccgaca gatggttgga
    51601 atggtttttc aaaatccaga caatcagttt gttggttcga cagtagaaga tgacgttgct
    51661 tttggtcttg aaaatcaagg gattcccaga gaagaaatgc tagtgcgtgt gaaagatgca
    51721 cttgagaaag ttagaatggc tgagtttgct tcacgcgagc ctgctcgttt atcaggaggg
    51781 caaaaacaac gtgttgccat tgccggcgtg gttgccttac gacctgatat cattatttta
    51841 gatgaagcaa ccagtatgct agatccagaa ggtcgtgaag aagtgatctc tacgatcaag
    51901 aagatcaaag aagaaagtca attgaccgtt atatctatca ctcatgatat tgatgaagca
    51961 gctaatgcca atcggatact agtgatgaga caaggcgaac ttgtccgtga aggtacacca
    52021 aaagaaattt tttctgctgg tcctgaactg atcgacttag gtctagatct accatttcct
    52081 gaaaaactga agagcgcact gaaagaacgt ggggttgatg tgcccagtga atatatgaca
    52141 gaagaaagga tggtggattg gttatggaca tccgttttga acaagtagac ttcacttatc
    52201 agccgaatac accatttgaa caaagagcct tatttgacat caatatgacg atcaaagaaa
    52261 acagttatac tgcattagta gggcataccg gaagcgggaa atcaacctta cttcagcatt
    52321 tgaatgcgct tgttaagcca acaagcggta cagtccatat tggagaacga gatattcagc
    52381 cggatacgga taataagaat ttaaagccca tccgaaaaaa agttggcatc gtctttcagt
    52441 ttccagaagc acagctattc gaagaaacgg tagcaaaaga cattgctttc ggtcctaaaa
    52501 actttggtgt cagcgaagaa gaagcactag tcctagcaaa agaaacatta gaacaagttg
    52561 ggctggatga aagctatttg gaacgttcgc catttgaact ttcaggaggg caaatgcgta
    52621 gagtagcaat cgctggtgtg cttgccatga ggccggaagt acttgtattg gatgaaccaa
    52681 cggcaggact tgatccccag ggtagaaaag aaatgatgga gatgttttgg cgtcttcata
    52741 aggaacagca gatcactatt gtattagtta cgcatttaat ggatgatgta gccaattttg
    52801 cagattatgt ctatgtgttg gaaaagggac gaatcgttaa tagcggggag ccgcaagagg
    52861 tctttcaaaa cattgaatgg ctaaaagaaa aacaactagg cgtacctact gccaccgagt
    52921 ttgcagaaga attaatggct aaagggatga actttgctac cttgcctttg acagctgaag
    52981 aattagcaga cgctatcgtc cattatgctg gaggaaatgc ccatgatgaa taaattgata
    53041 tttggtcgtt atatgcctgg agattccttg atccatcgat tggatccccg agctaaatta
    53101 ttagccagtt tttattttat cggaatcatt tttttagcaa ataattggga aacgtacctt
    53161 ttgttggcat ttttcacatt actttctgtc ttcttgtcaa aagtagattt gaaatttttt
    53221 atccgaggca ttcgcccatt gatctggcta atcctcttca ctgtagcttt gcaggtcttg
    53281 tttacaagtg gcggagaaac ttattggagt tggggtgttt tccatatcac tgaatttggt
    53341 gtgatgaacg gcctctttat tttctgtcga ttcgtgttga tcatttttat gtcgactcta
    53401 ctcacgctta ctacaccgcc tttggaatta tcggatgcga ttgagtactt gcttcgtcca
    53461 ttgaaagccg tacggttccc agtccacgaa atttcattga tgctgtcgat tgctcttcgt
    53521 tttgtgccta cattgatgga cgaaacagaa aagatcatga acgctcagcg cgctcgcggt
    53581 gttgattttg gtgaaggaaa cctagttcag aaaatgaaag cagtcattcc tttgctgatt
    53641 cctttatttg tcagcagctt caaccgtgcg gaagatcttg cgactgctat ggaagcaaga
    53701 ggctacaaag gtggggaagg aagaacgaaa tatcgtgtat tgcattggca taatagagac
    53761 acgatggtta tcattgcttt tgtattatta actgtgctac tgatcttttt gagaggataa
    53821 acgttcagtg ggccgctatc tattcttttc ttgctgatct gtttgcaaga aaggatgcga
    53881 gcagtgtatg acaagaaata ttagggtctg tggcagacta aggtcacagg cctctttctt
    53941 ttatctgaaa agaaagaacg aaaaaatttt ataaaaaaat cattattcca gataaccttt
    54001 taggaacaaa ggagagaaaa acatggtacg ttacaaagca atcattgcat acgacggaac
    54061 aaattttaat ggattccaaa aacagccaaa tgggcgtact gttcaagaag aagtagaaaa
    54121 aacattacaa aaaatggcaa atggaaaaga aatcacggtc tttggttcgg gaaggacaga
    54181 tgccggagta catgcgattg ggcaagtaat ccattttgat tatccagagg agcgtccgtt
    54241 agaacgaatg cgtttcgcgc tagataccca gtcaccagag gatattgctg taagacaagt
    54301 agaaattgtt tcagaagact tccacgcaag atatttggtc aaagaaaaga cgtatcaatt
    54361 tcgagtggat atagggaaac cacgcagtcc ttttcgacgc cattatgcaa gttattttcc
    54421 ttaccctctt gatttggaaa aaattcagcg ggcgcttcct gatttattag ggactcacga
    54481 tttcacctct ttttgtgcat caggaagctc gatagaggat aaagtacgta cgatctacga
    54541 ggctaagatg gaggtaaatg aaacaaaaga cgaattgttg tttacattta ggggaaatgg
    54601 cttcttgtat aagatgatac ggatcttagt aggaacgctt ttgaaaattg gcaatggccg
    54661 attgcctgaa gatagtatcc cagatattat tgcgaaaaaa gatcgtaatg cagcaggacc
    54721 tacagcacat cctgaaggac tttatttgta tgaagtgaaa tatgaataag aggttgtgac
    54781 agaagtggtc agcttcaaga aataagaagg aatttacgaa aattacttct caaaattttg
    54841 caaaatttcg gcttattttc gaaggagcta cttctggaac accgtgaatc agaggttgtg
    54901 aaaaagctgc cctgcatcaa gtaataagaa caaccaaaca aaaatagctc gtcatatttt
    54961 tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta aggaagaggt
    55021 tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa cggaaaaata gttcctcata
    55081 ttttgccgtt tgttgagcta ataccgaagg tcagctgatt gaacaccgtt taagaaagag
    55141 gtcgtgagcc tgtcatttaa ctctgagtca caagtaacaa tttcaaaaaa cagcttctca
    55201 tctttgatga atatcgtgac ttgtggacga ggcgttgata agtgaatcat ctttttgtta
    55261 cctatttttt ctgtaaccaa aaaacgatgg aacgttgttt tgattgccct taatgaggac
    55321 agtcggaaca ggtttgtcat ttttatattt aaatggcaca aatttttcta attatttgtc
    55381 atttagcgct gaaaattttt gctatttatc taaaaaagtg ataactactt aagatgaagc
    55441 agaatattta cgccatacgg catacaaccg cgaaaaatat aaaaaaaaga aaacgattga
    55501 acgcgttttt gcagtcgcaa aggaaaagca tggaatgcgc tacatcaagt atcgagactt
    55561 ggaaaacaat caggtgtata cgctgattgt ttttgctgca atgaatctga aaaagttgtc
    55621 cacttggcag ttggaacata tgtcttcata tcttttatta tacaaaaata gcaaaagcac
    55681 cagtctacac tcttagtaaa gaggaaactg atccttttgt ctacattctt aaagtactta
    55741 aactaagtac tttattttac tctttaggat tgatacagta gcatctatcc ctttattaat
    55801 aatcttttat ttttttaaat aaagatgttg tctccatctt caggcactaa aactttttcg
    55861 gaaattcctt tttctttatt gaaagacttt aattcttctc ttgacagtcc ccaatggttt
    55921 acagcttcca tatgaacggc aataattgtg gaatctggta ttgctttatg caattcatat
    55981 aaatcgtctt tccccataat aagagattct ccaactaaaa aatgattatc tccagcattt
    56041 gctactatga tttctggatt atactgatca atagtttgtt taacagcttc ataccaaact
    56101 gtatctcctg taaagtatag tgtcttctca gtcgaatgtt tgaaaacaat accgcaaaca
    56161 ttacctacat aattcaagat ttctcctctg ccatgtttgc cttttgtttt aaccaattgt
    56221 atagattcaa aaactgtgtc ttctcgtagt acctccacat ttttaaaacc atatagtcga
    56281 atttcattcg catcattttc atcttgcaca aataatttta tttctttagg taggacctct
    56341 ttcgctatat cgtcccaatg atctagatgt aaatgtgtta caataacagc atcaataccg
    56401 ttgataatat cttcgactgg cattggcaaa tcaactagag gattcatttg atcttttctt
    56461 ggtgatggaa aaggaccttt cgaaataaat ggtggaagtg ttcctttttc cgctaacatt
    56521 ggatcaatta aaaatttttt acctccatag tctaagatag ctgttgcatt acgaatgtga
    56581 tgtatattca taaacgttgt ctcctttttg ttgatatgaa atatttttag tataattcaa
    56641 ttccatacaa taaataaacc gaacaaaaaa agtcttttac tatttttact taatttatga
    56701 aaggttgttt accatgttag accaaacaga tataaatatc attgaggaac tatcaaaaaa
    56761 tagtcgaatc acaatgaaag aacttggaga aaaagtacat ttgactgga
//
LOCUS       DAYYCW010000006        56594 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000006 DAYYCW010000000
VERSION     DAYYCW010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.006"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..672
                     /locus_tag="ACHAYA_000349"
     CDS             154..672
                     /locus_tag="ACHAYA_000349"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8604184.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            800..1483
                     /locus_tag="ACHAYA_000350"
     CDS             800..1483
                     /locus_tag="ACHAYA_000350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM8604185.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1792..1865
                     /locus_tag="ACHAYA_000351"
     tRNA            1792..1865
                     /locus_tag="ACHAYA_000351"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1826..1828,aa:Arg,seq:tct)
     gene            2024..2383
                     /locus_tag="ACHAYA_000352"
     CDS             2024..2383
                     /locus_tag="ACHAYA_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM8604186.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2506..4137
                     /locus_tag="ACHAYA_000353"
     CDS             2506..4137
                     /locus_tag="ACHAYA_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM8604187.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4199..4840)
                     /locus_tag="ACHAYA_000354"
     CDS             complement(4199..4840)
                     /locus_tag="ACHAYA_000354"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8604188.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    5026..5102
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5122..5430
                     /gene="rplU"
                     /locus_tag="ACHAYA_000355"
     CDS             5122..5430
                     /gene="rplU"
                     /locus_tag="ACHAYA_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM8604189.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5457..5801
                     /locus_tag="ACHAYA_000356"
     CDS             5457..5801
                     /locus_tag="ACHAYA_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM8604190.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5814..6107
                     /gene="rpmA"
                     /locus_tag="ACHAYA_000357"
     CDS             5814..6107
                     /gene="rpmA"
                     /locus_tag="ACHAYA_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM8604191.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6541..9348
                     /locus_tag="ACHAYA_000358"
     CDS             6541..9348
                     /locus_tag="ACHAYA_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8604192.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9539..10030
                     /locus_tag="ACHAYA_000359"
     CDS             9539..10030
                     /locus_tag="ACHAYA_000359"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8604193.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10133..11122
                     /locus_tag="ACHAYA_000360"
     CDS             10133..11122
                     /locus_tag="ACHAYA_000360"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8604194.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11155..11958
                     /locus_tag="ACHAYA_000361"
     CDS             11155..11958
                     /locus_tag="ACHAYA_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM8604195.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11979..12893
                     /locus_tag="ACHAYA_000362"
     CDS             11979..12893
                     /locus_tag="ACHAYA_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8604196.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12976..13350
                     /locus_tag="ACHAYA_000363"
     CDS             12976..13350
                     /locus_tag="ACHAYA_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM8604197.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13489..13734
                     /locus_tag="ACHAYA_000364"
     CDS             13489..13734
                     /locus_tag="ACHAYA_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8604198.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13906..14970
                     /locus_tag="ACHAYA_000365"
     CDS             13906..14970
                     /locus_tag="ACHAYA_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM8604199.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15216..15641
                     /locus_tag="ACHAYA_000366"
     CDS             15216..15641
                     /locus_tag="ACHAYA_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8604200.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15634..16083
                     /gene="nusB"
                     /locus_tag="ACHAYA_000367"
     CDS             15634..16083
                     /gene="nusB"
                     /locus_tag="ACHAYA_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM8604201.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16180..17034
                     /locus_tag="ACHAYA_000368"
     CDS             16180..17034
                     /locus_tag="ACHAYA_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM8604202.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            17035..18372
                     /gene="xseA"
                     /locus_tag="ACHAYA_000369"
     CDS             17035..18372
                     /gene="xseA"
                     /locus_tag="ACHAYA_000369"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM8604203.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18377..18601
                     /locus_tag="ACHAYA_000370"
     CDS             18377..18601
                     /locus_tag="ACHAYA_000370"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM8604204.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18603..19493
                     /locus_tag="ACHAYA_000371"
     CDS             18603..19493
                     /locus_tag="ACHAYA_000371"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8604205.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19502..20320
                     /locus_tag="ACHAYA_000372"
     CDS             19502..20320
                     /locus_tag="ACHAYA_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM8604206.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20427..20876
                     /locus_tag="ACHAYA_000373"
     CDS             20427..20876
                     /locus_tag="ACHAYA_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8604207.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20894..22576
                     /gene="recN"
                     /locus_tag="ACHAYA_000374"
     CDS             20894..22576
                     /gene="recN"
                     /locus_tag="ACHAYA_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM8604208.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22631..22747)
                     /locus_tag="ACHAYA_000375"
     CDS             complement(22631..22747)
                     /locus_tag="ACHAYA_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM8604209.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22820..23776)
                     /locus_tag="ACHAYA_000376"
     CDS             complement(22820..23776)
                     /locus_tag="ACHAYA_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8604210.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23947..24315
                     /locus_tag="ACHAYA_000377"
     CDS             23947..24315
                     /locus_tag="ACHAYA_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM8604211.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24485..24916
                     /gene="mraZ"
                     /locus_tag="ACHAYA_000378"
     CDS             24485..24916
                     /gene="mraZ"
                     /locus_tag="ACHAYA_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM8604212.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24937..25896
                     /gene="rsmH"
                     /locus_tag="ACHAYA_000379"
     CDS             24937..25896
                     /gene="rsmH"
                     /locus_tag="ACHAYA_000379"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM8604213.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25901..26302
                     /gene="ftsL"
                     /locus_tag="ACHAYA_000380"
     CDS             25901..26302
                     /gene="ftsL"
                     /locus_tag="ACHAYA_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM8604214.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26303..28495
                     /locus_tag="ACHAYA_000381"
     CDS             26303..28495
                     /locus_tag="ACHAYA_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8604215.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28521..29483
                     /gene="mraY"
                     /locus_tag="ACHAYA_000382"
     CDS             28521..29483
                     /gene="mraY"
                     /locus_tag="ACHAYA_000382"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM8604216.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29497..30858
                     /gene="murD"
                     /locus_tag="ACHAYA_000383"
     CDS             29497..30858
                     /gene="murD"
                     /locus_tag="ACHAYA_000383"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM8604217.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30884..31972
                     /gene="murG"
                     /locus_tag="ACHAYA_000384"
     CDS             30884..31972
                     /gene="murG"
                     /locus_tag="ACHAYA_000384"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8604218.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31987..33207
                     /locus_tag="ACHAYA_000385"
     CDS             31987..33207
                     /locus_tag="ACHAYA_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM8604219.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33363..34688
                     /gene="ftsA"
                     /locus_tag="ACHAYA_000386"
     CDS             33363..34688
                     /gene="ftsA"
                     /locus_tag="ACHAYA_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM8604220.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34711..35952
                     /gene="ftsZ"
                     /locus_tag="ACHAYA_000387"
     CDS             34711..35952
                     /gene="ftsZ"
                     /locus_tag="ACHAYA_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM8604221.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35969..36646
                     /locus_tag="ACHAYA_000388"
     CDS             35969..36646
                     /locus_tag="ACHAYA_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM8604222.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36660..37259
                     /locus_tag="ACHAYA_000389"
     CDS             36660..37259
                     /locus_tag="ACHAYA_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM8604223.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37340..37561
                     /locus_tag="ACHAYA_000390"
     CDS             37340..37561
                     /locus_tag="ACHAYA_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM8604224.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37610..38392
                     /locus_tag="ACHAYA_000391"
     CDS             37610..38392
                     /locus_tag="ACHAYA_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM8604225.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38413..39120
                     /locus_tag="ACHAYA_000392"
     CDS             38413..39120
                     /locus_tag="ACHAYA_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM8604226.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39382..42180
                     /gene="ileS"
                     /locus_tag="ACHAYA_000393"
     CDS             39382..42180
                     /gene="ileS"
                     /locus_tag="ACHAYA_000393"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM8604227.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42229..43755)
                     /gene="zwf"
                     /locus_tag="ACHAYA_000394"
     CDS             complement(42229..43755)
                     /gene="zwf"
                     /locus_tag="ACHAYA_000394"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM8604228.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43770..44417)
                     /locus_tag="ACHAYA_000395"
     CDS             complement(43770..44417)
                     /locus_tag="ACHAYA_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8604229.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44601..44930)
                     /locus_tag="ACHAYA_000396"
     CDS             complement(44601..44930)
                     /locus_tag="ACHAYA_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM8604230.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45107..45835
                     /gene="sfsA"
                     /locus_tag="ACHAYA_000397"
     CDS             45107..45835
                     /gene="sfsA"
                     /locus_tag="ACHAYA_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM8604231.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45851..46864
                     /locus_tag="ACHAYA_000398"
     CDS             45851..46864
                     /locus_tag="ACHAYA_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8604232.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46864..48141
                     /locus_tag="ACHAYA_000399"
     CDS             46864..48141
                     /locus_tag="ACHAYA_000399"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8604233.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48204..50906
                     /locus_tag="ACHAYA_000400"
     CDS             48204..50906
                     /locus_tag="ACHAYA_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8604234.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            51058..51375
                     /locus_tag="ACHAYA_000401"
     CDS             51058..51375
                     /locus_tag="ACHAYA_000401"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8604235.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51405..51725
                     /locus_tag="ACHAYA_000402"
     CDS             51405..51725
                     /locus_tag="ACHAYA_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8604236.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51833..53293
                     /locus_tag="ACHAYA_000403"
     CDS             51833..53293
                     /locus_tag="ACHAYA_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8604237.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53361..53582
                     /locus_tag="ACHAYA_000404"
     CDS             53361..53582
                     /locus_tag="ACHAYA_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604238.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53613..53795
                     /locus_tag="ACHAYA_000405"
     CDS             53613..53795
                     /locus_tag="ACHAYA_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM8604239.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53795..54208
                     /locus_tag="ACHAYA_000406"
     CDS             53795..54208
                     /locus_tag="ACHAYA_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM8604240.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54331..55512
                     /locus_tag="ACHAYA_000407"
     CDS             54331..55512
                     /locus_tag="ACHAYA_000407"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8604241.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55583..55747)
                     /locus_tag="ACHAYA_000408"
     CDS             complement(55583..55747)
                     /locus_tag="ACHAYA_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604242.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55901..55974
                     /locus_tag="ACHAYA_000409"
     tRNA            55901..55974
                     /locus_tag="ACHAYA_000409"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55935..55937,aa:Arg,seq:cct)
     gene            complement(56043..56234)
                     /locus_tag="ACHAYA_000410"
                     /pseudo
     CDS             complement(56043..56234)
                     /locus_tag="ACHAYA_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT        18724 a         9540 c        12173 g        16157 t
ORIGIN      
        1 caattattta taataaaaaa tgagataaag atgaggaaat agaattagaa acatataatt
       61 attaaaggga aacattttta cagctataat ttgaaaaaat ttatcgttat tatatgatta
      121 agaaggattg aataaagaaa gaagttgaaa acgatacctg attacaaaaa aataactttt
      181 ataagttcta taacgttgat tatattttta attcttattc ttctcgtcgt cttggttccc
      241 cgaatttttg gtttgactcc ttatattatt gaggacaaag aaatgagtcc atattatgca
      301 caaggaaatc tgatatatgt acgaccgaaa aatacaaatg agattttagt cggagatatt
      361 attacatatt atgaaaattc aggaaaaaaa atctcgacgc gtcgcgtaat agccgttgaa
      421 aacaaccacg aagattttta tacaaaagct gatacaaaga cttatattga gcctggagta
      481 gtaaaaaaga gaaatattat cggtagtccc atctttcaga ttccatatat tggactagtc
      541 ctttcaaaaa cggcatttgc atggattcag gggttattct ttacgattgc tttttgccta
      601 acaggaatca cagcatggaa tacctttgta gagttgaaaa agaagcggag acgaacacag
      661 aaatttttgt aagaatgtta aaaacataaa tgtattccag agatccaacg actcttctga
      721 acatgaaaca cgagaaaaca ctcatgcagc ttgtagattt agtcagttgt tcgtataatg
      781 tgtgaagagg aggagtagta tgcgcaatga aatgatgcat cgtcctgtag tcaaagaaga
      841 actattggat tttatgcgta cccagcaaaa aaaattaccg ggtgagctag gcaaactgga
      901 agaagaagct catgtagcag aagtaccagt tattccccat gaaacagttg tatttttgaa
      961 atttttactt ggacagcttc agcctgaacg aatcttggag atcggagcag ctatcggttt
     1021 ttcatcaagt gtcatggcca cgacaatcgg agaaaacgct catgttacga caattgatcg
     1081 tttcgatgtc atgatcgaga aagctaaaaa aaactatgaa cgtctaggac tgacagataa
     1141 agtgactttg cttgaaggtc aagcagcaga tatcttgccg gagttgtctg gtccgtatga
     1201 ctttattttt atggatagtg caaaatcaaa atatattgaa tttttacctg aatgcttacg
     1261 tttgttaaga aaaggtggcg ttttgatggt agatgacatc ttccaaggag gaacgatctt
     1321 gctcccagat gaagagattc cccgtggcaa acgagctatc catcgtaaat taaatgaatt
     1381 tcttcgtgtt gtgatggatc atcccgattt gacatcgaca atcttgccat taggtgatgg
     1441 tgtgatctta atgacaaagg aatcagaaac aatcgagttg tgatacatga ttatggcagc
     1501 ctgtagataa tcgattgaat cgagggctca attcgaactt gatgtaaaag tttctatatt
     1561 tgtaagtcaa ttaaaggata caggaaatct aaataaacag gagtagcatg aagtcaatgt
     1621 agctcctgtt ttcaattgat aaccttagaa cagctgaagg agaatgtctc gttttaaaag
     1681 attgatttag tagttgggcc aaaataattc attatttgct aattaaataa ctatttcgta
     1741 ttgacagaag caatttaatt atctataatt attgatgttc cgctgattct tgtcatagta
     1801 gctcagctgg atagagcatc cgccttctaa gcggacggtc gggggttcga atccctcctg
     1861 tgacgctaaa aaaccgttag aaactgaggt ttctaacggt ttttctttgt tttttgtgcc
     1921 aatattgttt gttttcaaag gcaaataaaa taaaaaaaca tccttatcga gtacaattat
     1981 aaggagcaaa aaaataaaac tcatcagaaa agaggaagaa gatatgacga tactatctag
     2041 aattttagtg actctagttg caattgaatt tttttatatc atgtatatcg aaacggtgag
     2101 aactgattct gacacgacaa gtagggtttt taaaatgagt aaagaagagt taagcagaaa
     2161 atctgttcaa acattgttta agaaccaagg agtgtacaat ggactattag gagtgggact
     2221 actttatgga gcttatcttt cttccgcatc taaagaaatc acaagtatgc tattaatatc
     2281 tatttttttt gttgcattat atggcagttt gactagcgat cgttggattt tactcaaaca
     2341 aggtgggcta gctctgttgg cattgctttc tattttctta taaatataga aaaaataaga
     2401 atttatctaa caggaaactt tgtgaaaaat attactttat ctcatgaaat atgatattct
     2461 aatgaatggt attttagaaa atttcatgaa aaaggagtta gataggtgtt caaaaattat
     2521 gtttcctcac taaaaaaaga attttcaggt tatgatcttt caagatttca gaaagatctt
     2581 ttggctggga tcacagtagc tgctgtagca ttgcctttag ccttggcttt tggggtatca
     2641 agcggagctg atgcggccgc aggtttgatt acggctatca ttgccggtat cgttattggc
     2701 ggtttatcag gcggttttta tcaaatatca ggtccgacag gtgcgatggc agctatcttg
     2761 atgtctattg cggcaacaaa gggaatgcaa ggtgtattgc ttgctacttt tttagcgggt
     2821 ctatttttac ttgcagcagg acttttccga ttaggaacac tgacttcttt tatcccagca
     2881 cccgtaatta cggggtttac ttcaggtatt gctatcatta ttgcattagg tcagatcgat
     2941 aatttatttg gtgttcactc ggaaggtaca aacgttattg aaaaactaag tagttatcaa
     3001 acgataggat ttccgataaa cacagcctct ttgatcatgg gagggctagt cattttaggg
     3061 atgtttatct atccaaaaaa atgggcgaaa cgaatgccta gttcgctgct ggctatcatt
     3121 ttggcaacag cagttatgct attgttccat ttaccagtag caactgtggg taagattcca
     3181 caaacattga tcagtagtaa tcgtttgaat atgggagatt ttagcttttc tgccttgcaa
     3241 caagtggcag ttccagctat tagcattgct ttattaggaa tgattgaaag cttgctatgt
     3301 ggtgcttcgg ctggccgaat ggcaaataag cccttagata gcaatcaaga attaatggcg
     3361 caaggaattg gaaatctgtt gcttcctttc tttggcggga ttcctgctac tgcggccatc
     3421 gcccgtacaa gcgtggccat caaatctggt gcacaaacta gattagctgg tatgatacat
     3481 gcagttgtct tactgctctc catgcttgtt tttgcgccag tgatgtcaca gtttccaatg
     3541 ccagcacttg caggtgtttt gattgtgacg gcttggcgta tgaatgaatg ggaaacgatc
     3601 aaggagctat ttactaataa atattggtca gcagttctat tgtttatcct tacgatgatt
     3661 tgtacagtta tctttgattt aagtattgct atcgtgatcg gggtgatcgg cggctgtgta
     3721 ttttttgttg tcaaaagcgc tgctatcacg atttcattag aaacgatcga ttggcaaaga
     3781 atggggctgc cagaaacgga aaagctgaat aactggacag tagtgtatat tagtggtcca
     3841 ctgtttttta tgtcttcaga aaaactcaaa aataaattgg aagaactaaa tcatcacgat
     3901 ggtgtcattt tctccatgcg aggcgtacca agtattgatt tgacggcacg tgcagttttt
     3961 gatgaatttc aagaaaaagc agagcaaaga ggacaaacga ttatctatac ttccttgcag
     4021 ccagaagtag aaaaacaact ttctcacttg tgggaacata cattcacgca tcaacatcaa
     4081 acagttgctc atgcgttgac agctttacat gcgaaatata ctgcaattag ccattaaaac
     4141 aatgaaaaag aacagacaag actgtaaact gaagatttta tggtcttgtc tgttcttttt
     4201 aatattgctg taaaagtgaa gctagtactt gccagtcttg atggataaca ggtacaaggg
     4261 agcgattgta gaaaatagct gctggatgaa aaagtggaag aattcgatag tttttttctg
     4321 accattcgta ggcatccttt tgttctgtta attgtaagac aggccctgta tagagttgac
     4381 catgattttt ggtaaccgta tattctttgc caagtaaacg ttgaagacca atgtttccaa
     4441 gtgtcgcaat taattttggc tggaccttca tcagttcgta atccaatatt ggtgcatgcg
     4501 caaatacttc ggaacgagta ggtgtccgat ttggataaac agtttctgac tgttgtgtcc
     4561 gtttattgat ccgatgagtc acgcgatacg ggcggctcct tacagcactg gtgatataca
     4621 cctcttctcg tgttaagcca gttgaagaaa gtgcctgcat caattctttt ccagcttgac
     4681 cgctaaaagg aacatgcgta tcaatctctg ttttccctgg ggcttctccc acgagcatca
     4741 gtttaggatg aagaggtcct tgcccagcga taaatccttc tacaggacgg ccgttcattc
     4801 ttaaagaagc ttcttgtatc agttcttttg ggtattccat ttatgctcct cctttttctt
     4861 ttattataag aataaaagaa acacaatgcg actattttat ttggtagaac tgtgaggata
     4921 gaaaagcaga agtattgctt gaatttttgt tttacttttt gattttaaat ttagttgact
     4981 aacggttgct gttctggtaa gctagtactg ttgtaattgt ggactccaca gctacaaccg
     5041 ctcagaacaa gtaataagca cagcatatgc tgtcacttcg gatggcgagt ctaagtatat
     5101 cagaaggagg tgctttatag catgtacgca attattaaaa ctggtggtaa acaagtaaaa
     5161 gttgaagtgg gacaagcaat ctatgttgaa aaacttgacg ttgaagcagg agaaaaagtt
     5221 gtttttgacg aagttatctt agtaggcgga gaatctacaa aagtaggcgc tccaactgtt
     5281 gcaggtgcaa ctgttgaagg tactgttgaa aaacacggca aacaaaagaa agtcgttact
     5341 ttcaaataca aacctaaaaa acattcacac cgtaaacaag gtcatcgtca accatataca
     5401 aaagtggtta tcgacgcgat caacgcttaa tagcgtttga gaaagaaggc ctatatatga
     5461 tcaaaggaac atttaagcga aatgacgcag gtcagatcgt ttcattcact ttgaccgggc
     5521 atgctgatgc tggtccttat ggaagtgata tcgtttgtgc gggtgtttct gcgttagcga
     5581 tcagcactgt caacgggatt gcctcattag ccggttttga gccgattgtt gaaatgaatg
     5641 aagaagaagg cggttatctt tataccgaag tcacttcagg aatgacacaa gaacaaaaca
     5701 atattgctga gattctatta gaaaatcttt tattaggatt acaatcgata gaagctgaaa
     5761 actcagaata tatccaaatt aaaacaatta atgataaata ggaggtgcag accatgttgt
     5821 tatctatgaa tctacaatta tttgcccaca aaaaaggtgg cggttctaca tctaacggcc
     5881 gtgactcaga atcaaaacgt ctaggtgcta aacgtgctga tggacaaaca gttacaggtg
     5941 gatcaattct ttaccgtcaa cgcggtacaa aaatctatcc aggtgtaaac gtgggtatcg
     6001 gtggagacga cactttattt gcaaaagttg atggcgtagt acgtttcgaa cgtaaaggcc
     6061 gtaacaaaaa acaagtgtct gtttatccag tagctcaaga agcttaattg aatcttatga
     6121 ttaaagaggt tgtgacaaga cttttgtcac agcctttttt gtttataaaa ataaaagttg
     6181 tagtggaggc tacgaagttt actttgtctt tttacactta atattttttt gccaatttgg
     6241 aatcaggatg ttttgataac tatcgttttc aattgatgga agctatattg tttgctatca
     6301 gtgaaataat tatatggtaa acagtgttct ttttcttgtt tccattataa aaatcgtatc
     6361 gggatttttc gttcttttaa attgaacatt tctatctatc cgcgtgttat caacaaaaca
     6421 aaatcaggta aatagttgac gaaacagaaa taaatacata gtattatgtt ttgaagccta
     6481 agtacaatag tctatagaaa aggagcgata ccattggcaa aacgcattga ccgaatttat
     6541 atgtatgtca aagaaaaaac cgctcattta acaaaggcag aatatgatca aggagtaaca
     6601 acacaggaaa ttgctgaagt gttagggatt cagcgaacaa atagcagtaa agatctgaat
     6661 cagctagtgc gcgaaggaaa actgataaag acagacggac gaccagtacg ttacatatat
     6721 caaacactcg ttacgcacgt aaagccagtt acaaaacatg tagtcagtta caaagaaacg
     6781 ccggtctctg aagaaaaaaa tacactccaa atagatacta aggatatctt tgcaaaaatc
     6841 attggagcta acggcagcat gaaaaattca gtggaacagg caaaagctgc gattttgtat
     6901 ccgccaagag gattaaattg tttgattaca ggacctaccg gatcaggaaa gacttatttt
     6961 gcacacgcga tgtatcattt tgcaaaagcg catcatgtaa tagatgagga aacggaactg
     7021 attgttttca attgtgctga ttatgccaat aaccctgagc ttttgatgag tcatttattt
     7081 gggtacgcaa aaggtgcttt tacaggcgca gatgaagaaa aaatggggat tatcgatcaa
     7141 gcggatggcg gtatgctgtt tttggatgag attcaccggt tgccgcctga aggtcaagaa
     7201 atgatctttt actttatgga tcatggaact tacagtcgtc ttggggaaac aacgaaatcc
     7261 catgaagcaa atgtacggat cgtaggtgcg accacagaag acccaggttc ttctctatta
     7321 gaaacttttg ttcggcgtat cccaatcaat atcaaacttc cggcttttga aaaacgtcct
     7381 gcaaatgaga aagtcgattt agtaaaaatc atgattgccc acgaagcaaa ccgcacgcag
     7441 cgaaagattt ctttgacaga agatgtagca aaagcattga tcggcagtgt gacctatgga
     7501 aatataggac aattgaagtc aaatatccag ttagtatgtg cacgaggatt tctaaatcat
     7561 atgcaatcac ccgaaatctc gatcacaatc gatgatttga cagaagggat tcgtagcgga
     7621 ttgattcaat tagcaagcaa ccgcagtgca atggcagagc tttctaaaat attggagcca
     7681 aagataactg tagcaccaaa tgatacgatc atgaaaatcc aatcggactc ctatgagtta
     7741 ccttataatc tatatgatat catcggagat aaagcagctt tactgaagtc ggatggtttg
     7801 gatcaagaag caatcaatca tttcatttct acagatatca acatccatct gaagtctttc
     7861 tataaagacc atggtttttc ttttaatgca gacagcaaat tagcagaatt tgtagatccg
     7921 aaaatcatag aagtgaccaa tcagatttat gtaatggtca aaagatcttt accatatgag
     7981 tttcagcaaa actttattta tgctatgagc ttgcatatca gttctttttt gaaaaaaatc
     8041 aacttaggag aagaacggca tacgaatgat aacatccgtg agatggcttt tgattttcca
     8101 gatgaatatg ctgttgcgaa agaagtgcgt agttatatcg aaacgtattt tcaagtggag
     8161 attccagaaa gtgaagacta ttacttgacc gttctactcg tctcgttacg ggcaaatcaa
     8221 gcaagcggca gaatcggtgt agtcgttgca gctcacggaa acagcacagc aagcagtatg
     8281 gtccaagttg tccaacagtt gttagatgca gagaacgtac gagcagtaga catgcctttg
     8341 gatatggatc cgaaaacagc attggaccga attgaacgca atgtacaaga agtagatgaa
     8401 ggaagcgggg tcatactctt agtagatatg gggtcccttg cgtcgtttaa ttcgcaaatt
     8461 caaagagaca ctggaattcc agtacgtact gttgatatgg taacaacttc tctagttttg
     8521 gaaactgtac gaaaggtctc agtattaggg acagatctag atatgttgca tgaatcattg
     8581 aaaaattttc gaggttacgc agaaatatct tctgtagaat ctgttcagga agcaaaacct
     8641 attcataaga aaaaagctgt tttggctatc tgcgcatctg gcgaaggtac agcacaaaga
     8701 atcaaagaat tgatcgaacg agcggtaaac aagcgtcaag aaacagaatt agaagttcta
     8761 gctttgtcga ttgtggaagt gaaagaagaa ctgccagtta tccaaaaaga atatcagatc
     8821 atagctacta ctgggattac tgatttgaat atccaagccc catttattcc tttagaacgt
     8881 tttattgatc aaaatatcga agtcattttg gatcagttgc tgatggaatc agaattggag
     8941 gaaactgaat tcatctccct agatgaagag tcagctaaaa atacgtgtgt tgagtttatc
     9001 tctgataact tcatctttat taatggtagt aagttaatcg atccaatgtg gcaattttct
     9061 acacaaatct ctcagacgac gggaatcggc gatgaagaat atggattcaa aatcaaccta
     9121 gtgatgcaca cagcaggaat gatcgaacgt attattcgga atgaaccatt gactgtggaa
     9181 gaaaacgaat tgacaaatac aacgaatgat cctctttata gccaattggc agcctcagtt
     9241 gttctgttgg aagatcagat aaaagtaaaa gtgccgatag aagaaatgta ttatctgcta
     9301 cgtctagtcc ataatcagtt agataaaaaa gaatacactg tgccatgaat acacaaaaac
     9361 gatacacttt tatagtgtat cgtttttgtg tattggttaa gaatccttta aattcaatta
     9421 gtgtatcgta aaaaagttgg cacgcttttt gcttatagta gtgtgtgcta ggaggtggtt
     9481 gttcacacag aaatgatagg ggtttctgtg aagtagtgag gaagaaggag ttttgcagat
     9541 ggatattcga ttggcacgta tagatgatcg tttaatccac gggcaagtcg caacagtttg
     9601 gtcgaagcaa acaggtgttg aacgtatcat tgtgatcagc gatgtagtcg ctcaagataa
     9661 attgagaaag tttctactaa aagaagctgc accgccgggg atcacagcaa atgtaataac
     9721 cgtgcagaaa atgattcaaa tctatcatag tggattatta tcacgagcaa aagtgatgct
     9781 tttattcacc aatccccaag atgttgaagc ggttgtccgc tgcggtgtac agttgaagtc
     9841 gttgaatatc ggcggtatga gttttactga aggcaaatca atgattacca attttgtttc
     9901 agtaaatcaa caagatattg attcctttcg ttacttgaac agtcaagaag tggagttaga
     9961 gattcgaaaa gtacctgctg acaggaaaat ccttttgatg gaactactag cgaaaatcaa
    10021 aaaggtgtag caagaatggt tgttggaaaa ggtcaaataa ttctgttgta atggttagca
    10081 attattaata tttttgaatt tgttacaaca aaagtaaata ggaggtataa atatggtagg
    10141 gattatccta gcaagtcatg gacaatttgc tgaaggaatc ttacaatccg gttcaatgat
    10201 ctttggcgaa caagaaaacg tcaaagccgt gattttgaaa ccaagtgaag gtccagatga
    10261 cttgagagcc aaattggaag aagcggttgc ttcttttgat aatcaagacg aagtgttatt
    10321 cttagttgat ttatggggcg gaacaccgtt caatcaatca aacacattat tcgaagagca
    10381 caaagataaa tgggcaatcg tcagcggttt gaatttacca atgttgattg aagcctacgc
    10441 atcacgcttt tcaatggaat ctgctcatga aattgcagca cacatcattg aaacagcaaa
    10501 agacggtgta aaagtaaaac cggaagaatt agaaccggca gaagcaccga aagcagcagt
    10561 tgaagacgca caacctaaag gagcacttcc agaaggaact gtggtcggag acggaaaaat
    10621 caaatatgta ttagcacgtg ttgactcacg tctgttacat ggacaagttg cgacagcttg
    10681 gacaaaagca gtgcaaccaa accgtattat cgttgtttca gatgcagtat caaaggatga
    10741 tctgcgtaag agactaatcg aacaagctgc tcctccaggt gtaaaagcaa atgttattcc
    10801 tattagcaaa atgatcgaag tagcaaaaga ccctcgtttt ggaaatacca aagcattgct
    10861 tttatttgaa aatcctgaag acgtattgac agctgttgaa ggtggcgtag acatcaaaga
    10921 attaaatgtt gggtcaatgg cgcattcggt cggtaaagtc gttgtaagta aagtattgtc
    10981 tatgggacaa gaagacgttg aagcgtttga gaagcttgaa caaaaaggtg tgaaattcga
    11041 tgtacgtaaa gtacctaatg attcacgcga taacatggat gatattttga aaaaagcaaa
    11101 agctgaatta gcaaaagcgt aataaataat attaaaattt ataggaggct tatcatgtct
    11161 attatttcaa taattttagt cgtattagtt gcctttctag ctggtatgga aggtattctt
    11221 gatgaatttc aatttcatca accactagtg gcatgtacat tgatcggttt agttaccggt
    11281 aacctcgaag ctggtatcgt acttggcggt acgcttcaga tgatcgcttt aggttgggca
    11341 aatatcggtg ccgctgtagc accagatgct gcattagcat cagtagcatc agcaattatt
    11401 ttagttttag gtggacaagg tgttaaaggt gttccttctg cgatcgcgat tgccgttcct
    11461 ttggctgttg cagggttgtt cttaacaatg atcgtacgta caatcgctgt tccaatcgtt
    11521 catttgatgg atgcggctgc tgaagaagga aacatccgta aggttgaaat gtggcacatc
    11581 attgctgtat gtttacaagg ggttcgtatc gcggtcccag cagcagcgtt gctatttatc
    11641 ccagcacaaa ctgtgcaatc attccttgaa tcaatgccag catggttaac agatggtatg
    11701 gctatcggtg ggggaatggt cgtagctgta ggttacgctt tggtaatcaa catgatggct
    11761 acaaaagaag tatggccatt cttcgttatt ggtttcgttg tagctgcaat ttctcaatta
    11821 acattgattg ctctaggtgc tttaggtgtt gcattagctc ttatttactt gaacctttct
    11881 aaaatgggtg gttcatcaaa taatggaggc ggaagcaact ctggtgaccc tttaggcgac
    11941 attttgaatg actattaatc ttgaaggagg aaaataaaat ggcagaagaa aaaatcaaat
    12001 tatcaaaaaa agatcgttta gctgttgcgt ggcgctctac cttcattcaa ggttcatgga
    12061 actacgaacg tatgcaaaac ggtggctggg cattttctat gattccagca atcaaaaaat
    12121 tatacaaaac aaaagaagaa cgttctgcag cattaaaacg tcacttggaa ttctttaata
    12181 cacacccata cattgcttct ccaattttag gggttacatt ggctctggaa gaagaacgtg
    12241 cgaatggcgc gcctgttgat gatgtagcga ttcaaggggt taaagtaggt atgatgggtc
    12301 ctttagctgg tgttggtgac ccagtgttct ggtttacagt acgtccaatg ctaggtgcac
    12361 taggtgcttc attagctatg ggaggaaata tccttggacc aatcatcttc ttcttaggtt
    12421 ggaacattat tcgttgggca ttcatgtggt atacacaaga atttggctac aaagctggtt
    12481 ctaaaattac tgatgacctt tctggcggat tgttacaaga cgtaacaaaa ggtgcttcaa
    12541 ttttagggat gttcgtatta gctgcccttg tacaaagatg ggtatcaatc aaattcttac
    12601 cagttgtatc aactgtaaaa ttagataaag gtgcttacat cgaatgggat aaattgcctt
    12661 caggtggtga aggaatcaag agtgcatttg aacaagttaa taatggctta gccctttctc
    12721 ctgaaaaggt aacaacatta caagacaact tagaccaatt gattcctggt ttagctgcat
    12781 taggattaac attgttatgt atgtggttgc ttaagaaaaa ggtttctcca atcgttatca
    12841 tccttggttt attcgtagtc ggtgttgttg gacacgtcat cggactttta taatccataa
    12901 aaatatagct cgggcgttgt cccgagcttt ttttatatag tatactgttc ataaacaaat
    12961 atctggaggg agaaaatggt tcaatcaatc aatacaaaag tagacttggt aattgatgct
    13021 acttcgttta cgggtctgac agattacggg caaatcatga taggtaataa tggttttgag
    13081 ttttaccatg cgcgagatag acgcaaatat atccaaattc catgggaaga agtggattat
    13141 gtactagcat cagtcatgct aaagggaaaa tggattcctc gatatgccat tcaaacgaaa
    13201 aaaaatggta catacacatt ttcttccaaa gaacctaaaa aagtgcttcg tgcgatcaga
    13261 gaatatgtag atgaagatag aatggtacaa tcattgagtt tctttgatgt agtgaaacgt
    13321 ggtattcgta gtatgtttaa acgcaagtaa aaaataaaga aaacttctaa gaggaggttt
    13381 tctttatttt tttactttgc agaagaaaat atgaaatagc aaaaccaatt atttgtcccc
    13441 tttaaatccc atatgctaaa attgattagc aaagaaggag gttatattat gagttggtta
    13501 tggacattga ttgttggagc cgtgatcgga gcaattgcag gtgcgatcac cagtaaagga
    13561 aattcgatgg gctgtgtaac gaatatcatt gctggactag tcggttcaag tattggacaa
    13621 gcgattttcg gcgaatgggg accaaaagct gcaggtatgg cacttattcc atctatttta
    13681 ggcgcagtga ttttggtagc agttgtctca tttttctttg gaaaacgatc ataagttttt
    13741 acaaaatagt aaactaaaag gagttgcagc gagactgtga ctcctttttg gctttattta
    13801 tttaaaataa aaagaagttt tccggaaatt tttatttaac tgtcgtgtac gaagaaaagt
    13861 ttgctataat ggaaaaaagc gtgcaaagaa agagggagtt tcattatgat gctacgtgta
    13921 gaaaaattgc gcaaaaaaat gcaagaagaa aatttggatt catttttggt taccagtccg
    13981 tacaatcttc gttacttaac gaactttaca ggtacgacag gattagctgt tattactttg
    14041 gaaaaagcat tttttattac tgattttcgt tataccgagc aggcggcagc gcaggctcaa
    14101 gggtttgaaa ttattaaaaa tgtcggacct atttttgatg aggtagcaaa cttagtacgc
    14161 aaagaagaac tgcactcatt agcttttgag gaaacaacag tctcattttt agaatattcg
    14221 gttttagaag aaatcatcga tgcggaatta gtacctgttt cacagatgat cgaagaactc
    14281 agagaagtga aagatgaaga agaaatcgct attatcgaaa aagcatgtca tattgcggat
    14341 atggcatatg atcacatcct gaaaatgatc caaccgggca tgactgagat tgaagtcgca
    14401 aatcaactag acttttatat gcgttctctt ggcgcttcag gtgtttcttt tgatacgatc
    14461 gttgctagcg gattgcgttc ggctatgccg catggtgtag caagcaaaaa aatcatcgaa
    14521 caaggtgact tgatcacatt ggactttggt tgctattacg aagggtacgt ttccgatatg
    14581 acacgtacat tcgctattgg tgatcctggg gaaaaattaa aagaaattta tcaaatagtc
    14641 ttagatgctc aattagcagt tattgatgct gcaaaaccag gaatgactgg tgtccaatta
    14701 gatgcagttg ctagagatta tatttcaaaa catggttacg gggaagcatt tggacattct
    14761 acaggacatg ggatcggttt agagatccac gaaggaccaa acgtttcttc tcgtgcagaa
    14821 aagcaatttg taccaggaaa tgtgatcact gatgaaccag ggatctactt accaggaatc
    14881 ggtggagtac gtattgagga tgacttgttg atcacagaag atggaaatcg tgtgttgact
    14941 cgttcaccaa aagaattgat catcttatag tttcatcaat agaagaagta agcgtaaaaa
    15001 tcagtgttca cagactttgt tatagaaaat cactcgattt aatgatacac tatagaagaa
    15061 cttttaagct gtaagagata ataaagttgg gtgttttctc caattcatag cagtttgcag
    15121 ctgatcggcg gcagttttct taccacgtga tcactatgcc gcatcataga gaattcggat
    15181 gtatctcgtg cttaggcaaa gggaggaata aataaatggc agatgaaaaa aatttgattt
    15241 taaacgcaaa ccaagattta ggagagatcg tgatcgctcc tgaagtcatc gaagtaatta
    15301 tcgggattgc agcttctaaa gtagaaggcg tatacggaat gcgcggaact tttgccagca
    15361 atgtcactga attattagga cgtgcagctc acggaaaagg tgtttactta gcaaacgagg
    15421 aagatggctt gaaagtagat ctttactgtt atctagaata tggtgtttca gttcctaaag
    15481 tagcaatgga aatgcaggaa cgggtcagcc agcaagtcct tttcatgacg gatatcgatt
    15541 tatctgaagt caatatccat gtagtagctg ttgttcctga aaaattagaa caaccaacaa
    15601 tcgaagaact ttttgaagac gaagaggaag agaatgaata aagaattatc aagacacgag
    15661 attcgggaaa tggcattgca ggctttattt ccattagact tcaatgcaga cctaacaaaa
    15721 gaagatgcga ttttcaacgc aatcgaatta gaccaccgcg acatgatcaa cgaagatgaa
    15781 tcagagtttg ttcctgttta tcttgatacg ctagttggcg gtgtctgtgc aaaaaaagat
    15841 gaattggata aagtgattga aaaacattta aaaaacaatt ggcacatcaa tcggatctca
    15901 aaaatggatt tggtgatctt gcgtatcgcg atttttgaaa tgacttatgt gacagatgta
    15961 ccagcggctg ttgctttaaa tgaagcaatc gagttggcaa aaacatttag tgatgaccgt
    16021 tcacgtaaat tcgtcaacgg tgttttatcc aatgtattga aagaattaga agctggggcg
    16081 taagtcctag cttttttcta tgaatcatca taaacctgtt ataaatcggg aaaattctat
    16141 ggtagaatga aaacacaaaa aatatctggg agtggttaga tggcagaatt gattaacgga
    16201 aaagaattag ctgaaaaaat gcaggcagag attgctgaaa aaatccaaaa attgaaagat
    16261 aatggtatcc atcccggatt agtcgtttta ttggtagggg aaaatccagc cagccaaatc
    16321 tatgtaagaa ataaagaacg tgtagcaaaa gaaatcggaa tccattcatt ggtagagcgt
    16381 tatcctgaga cgatttctga agacgaacta cttgcagaaa ttgaaaaata caatcaagat
    16441 cctagttttc atggtatatt ggtccaatta ccattgccaa agcatataga tgaagaaaag
    16501 atcctgttag ctatccgccc tgaaaaagac gtagacggtt ttcatccgat gaatttaggc
    16561 agactccttg cgggtaaacc ggacaagatt ccttgtactc catacggcat catgaaaatg
    16621 tttgaagctt attctatcga tccggcgggt aaacgagcag tagttatcgg acgaagcaat
    16681 atcgtcggta aaccgatggc tcagctactc ttgatggcag atgcaactgt tactgttgct
    16741 cattctaaaa ccgaaaatct ggctgaactg gcacaagaag cagatatttt agtcgtagca
    16801 ataggacgtg gacattttgt cacaaaagaa ttcgtaaaac caggtgctgt agtgatagat
    16861 gtagggatga atcgtgatgc taacggtaag ctgatcggtg atgtgaaatt tgatgaagtg
    16921 gagcctattg caggctggat cactcctgtg ccaaagggtg tagggccaat gactatcacg
    16981 atgctgatgt atcaaaccgt aaaatctgcc gaagcttttg gagcaggtga atagatggca
    17041 caagaatatt taactgtcac tacattaacg aagtatttga aaagaaaatt tgatgccgat
    17101 ccttatttag aacgcgtata tttaacaggc gaaatatcga atttccgttt acgtgccaat
    17161 gcccatcaat atttcagctt aaaagatgac catgcaaaaa tatctgctat tatgttcaaa
    17221 ggagcattcc aaaaattacg attccagcca aaagaaggaa tgaaagtcct tgtcgttggg
    17281 cgtatttcat tatatgaagc gggaggttct tatcaaatct atgtagagca catggagcct
    17341 gatggaatag gtgctttata tcaagaattg gaagaacgaa aagaaaagct gatgaaggaa
    17401 ggcctttttc aagggcctaa aaaaatgctg ccacgtttcc caaagcgaat cgcggtcctt
    17461 accagtccaa gcggagcggt tatccgcgat atcatcacga cggtgaaaag aagatatcca
    17521 attgtccaat tggttttgtt cccaacagtt gtacaaggaa accaagcagc ggacaatgtt
    17581 gtcaggaata tccagagagt agaagagatg ggcaatttcg atacaatgat catcggtcgt
    17641 gggggtggtt cgattgagga tttatggccg tttaatgaag agcgggttgc tcgggcgatt
    17701 tatcaagcta atacgccggt gatttcatct gtaggacatg aaacagatac cacgatcgct
    17761 gatttagtag cagatgttcg ggctgccact ccaacagcag ctgcagaatt agctgttcct
    17821 gttttgtcag aagagttgat gcgcattcaa gaacggcaag ctagattgca gcaggcgttt
    17881 gtacgccaga tccagcgcaa acaagaacgg ttcgagcgtg ctcgacgttc ctatgtattt
    17941 cggcagcctg aaagattgta tgaaggtcag accatcaaat tagaccagtt gaaacagcga
    18001 ttatatcaag ctacacagca aatttatcat gcgaaagaaa aacaaacgat cgcattaagc
    18061 caccaattgg aacaagtggc tccgctttat cgagttaaag cagctcgcca agaaactgct
    18121 tatctagaaa aacgcttaga agagaaaatc caacaatata tgaaacaaca gcagcagcgt
    18181 tttgctcaag ctgttcaatc tcttgatttg ttgagtccat tgaaaatcat gggaagaggc
    18241 tacagctata cgacgttaaa tgatcaagta gtaaaaagtg tcaccgagat tcatcaaggg
    18301 gatttgctga cgatccatta tcaagatgga atcgttaaag gacaagtaca agagatagac
    18361 aaaggagaat aaaaatatgg aaaatccaac ttttgaagag tcactgcaag aattagaaaa
    18421 aattgtcatg cagttagaac aaggagacgt tccattagaa tcagctctag attcgtttaa
    18481 acgaggaatg gaattaagca aacattgcca agatacatta gctaaagcag aagagacctt
    18541 gacgaaaatg atgacagaat caaatgaaga agttgatttt gacggaaacg aggaaagctg
    18601 agatgagatt aactgacttt tcagctattc atctacctgc tgtagaaaaa gaaatcttgt
    18661 cttttttaga tgagcataca acggatagag gactctatga tgcgatgagt tattcagtga
    18721 aagcaggcgg gaaaagaata cgtcccttat tacttttaac ggccgttgct tcctttgatg
    18781 agcctattga tgttcccgtc tatcaagtag cagctgcttt agaaatggtg catacgtatt
    18841 ccttgatcca cgatgatttg cctgcgatgg acaatgatga tcttcgtaga ggaaaaccta
    18901 ccaatcataa agtattcggc gaagcattag ctatattggc cggagatggt ttgcttacag
    18961 gagcattcca gctaatcagt atggcacact taggtaattc tcctaagctt ctgctgcttc
    19021 agcaattagc tgtttgtgcg ggatcacaag gtatggttgc tgggcaagct gcagatatcg
    19081 agggcgaatc gaaaaagctt tctttagaag aattagcatt tattcatgaa agaaagacag
    19141 ggcatttgat tcgttatgcc ttgctggctg gtggcatatt agcgaagcaa cctgaagaaa
    19201 tcttattgct acttcaacga ttagccgaac atttaggttt agcttttcag ataagagatg
    19261 atctactcga tgtgattggt acaacgaaaa cattagggaa aacagctggt aaagatgaac
    19321 gtatggaaaa aaatacttac ccgcgtttat taggcctaga aaagacacgt gaggcgttgg
    19381 agattgagct gttatccgcc aacaagatca tagacaaatt agaagaggac gttttttctt
    19441 ttgatggcgg gctgatgcgc cagatgatca agcaatttga tgtggagaaa tgagggaaag
    19501 aatggaaaaa gaacgcgtag atgtattagc ggtcaaacaa ggattatttg aaacaagaga
    19561 acaagccaaa cgttcagtga tggctggttt gatttacaac gaaaaaaacg agcggtttga
    19621 taaaccggga gaaaaaattc cagtgagtag tgaattaaga gtcaaaggta aaaaacttcc
    19681 ttatgtttct cgagggggat taaaattaga aaaagcactc aaagcatttg atctatctgt
    19741 tgaaggaaaa acattgttag atattggtgc atccactggc ggatttactg atgcagcttt
    19801 gcaaaatggc gcaaaaatga gttatgcttt agacgttggc tacaatcagc tagcttggaa
    19861 aataagacaa gatcctagag tcgtcgtaat ggaaagagtg aattttcgtt atgcaaaacc
    19921 cgaagacttt accttaggaa ttccagaagt tgctgttatc gatgtttctt ttatttcact
    19981 aaaactgatg ctgcctccat tacatgctat tttaaaagaa gagggagaag tgattgcttt
    20041 aatcaaaccc caattcgaag caggcagaga agctgtaggg aaaaacggaa ttgttcgtga
    20101 ccctcagacc catcaaaaag ttttagaaga cattttgtca tttgcggcac aaggtggata
    20161 tgatgtgctg gaattaagtt attctccgat cacgggggga gaaggaaata tcgagttctt
    20221 ggctcacttg aggaaagtac ccgaaagcgg aacaatcaat tcagctatta atatggcaga
    20281 agtggtttca aatgcacacg aacagtttga ccataaataa attgtaactg tgaaaatcaa
    20341 gtgagaagta tgcggattct tctttattat accaaggaaa taagctatca ttgattaaga
    20401 agaatatgct aaggagcgaa gacgtaatga gaaaaaaaga tcggcatcgt ttgattactc
    20461 gattgttgag tgagcaagat atccgaaagc aggaagaatt tgttgaaatc ctgaaaagca
    20521 aaggaatatc tgttactcaa gctacgattt ccagagatat aaaggaactg aaactgatca
    20581 aggtaccggc ttcagatggc ggctaccgtt atagcatacc agctgaaaca aacgaagatg
    20641 taaatactaa attggaaaaa ctattaaaag acgctttcgt ggcagttgac caaatggaaa
    20701 aatttgttat tttgaaaaca ttgccgggaa atgcttcggc agcagcaaat ttgattgata
    20761 aacgttacaa gaaagaatta ttttcgatta tcaatgatga cgataatgtt ttgatgatta
    20821 cacgaactga agaccaggca gttttattga agaaagactt cctgcattac ctttaagttg
    20881 aggagcgaat gtgatgctac aagaaatcag tattactaac tttgcgatta ttccggaatt
    20941 gcggctttct tttcacgaag gaatgaccgc gctgaccgga gagactggtg cgggtaaatc
    21001 cattattatc gatgctttag gtctgttagc aggaggaaga ggttcaagcg attatatacg
    21061 tcaaggagca gaaaaatgtg tgcttgaagg gttgtttgaa ctaccaaaac aagaaggatt
    21121 ctctgaactg atggttgaat tagggatcga aacagatgag gacaatctga ttgttcgacg
    21181 ggatatgtcc ttaactggaa aaaatgtttg tcgagtgaat ggtcatatta tcacattagc
    21241 taatctgaga agaatcggca gttatttagt agatatccaa ggacaaaacg aacatcagga
    21301 actgctccag cctgaatcac atcttgcttt attggatcgt tttggagatg tagctttcca
    21361 acagaagaaa aagagttatc agcaagaata tatctcttat cgtgagctgg aaaaaagggt
    21421 acgaaaagtg cagcaaaatg aaaaatccta tgttcagaga atagatatgc tccatttcca
    21481 gcaagaagaa atagctgctg ctgatttgca agtcggagaa gaagagaaat taaaagaaga
    21541 aagagaaaaa cttagcaatt atcaaaaaat tgctgatgga ttagctgcag gttacggagc
    21601 actgacgaat aatgaacaaa acagtgtaga tggtgttggc ttggccgttt cagaactgca
    21661 agggatcgcc catctagatg tggagtatga agcaatttat gaaaatatcc aaagtgctta
    21721 ctatttgctg caagatgcaa tcggcgatat gagtcgtcag attgatttat tggagttaga
    21781 tgagagtcgt ttagaagaag ttacgcaacg tttagaatta atccgacagt tgaaacgtaa
    21841 atacggcgaa tccattgagt caatattggc ttactatgac gaaatcaccg aagagctagc
    21901 ttcttctgat ttttcagaag gtcaattgga caaaatgaaa gaggaactag agcaaaaaga
    21961 gcttctactt cagcaacaag ccgcagatct tcatgaagcg cgtaaaaaaa tcgcaaaaga
    22021 attggaaaaa tccattctgc atgaattgaa aagtctgtat atggaaaaca ctgagtttga
    22081 ggtacgtttc ttaaaagaag aaaatagaca attgaatcat gatggatttg accaaatcga
    22141 attttacatt acgaccaatc caggagagcc actgaagcct ttggtgaaag tcgcttctgg
    22201 tggcgaactg tctcggatgt tgcttgcttt gaaaactatt ttttcttctg agcaaggtgt
    22261 gacaagtatt atttttgatg aagtggatac aggtgtgagc ggaagagttg cacaggccat
    22321 cgcagataaa atttctcaaa tatcgaaata ctctcaagtt ttgtgcatca ctcatctgcc
    22381 tcaagttgct gcagtcgccg actatcaata ttatattgtg aaagcagtga ttgatggacg
    22441 tacgcaaact tctgtttcag agttgaaaac aaaagaaaga gaagaagaaa ttgcacgtat
    22501 gctggctgga agtgagatta caaaactcac actcgaacat gcaaaagaac tattgaaact
    22561 cgcaaaaagg acctgagcca cctcgcagtg gttcaggtcc ttttcagcag ctgatagcta
    22621 tattttattt ttacatgaca ctcattacag cagtcaatac aacagatcca atcgttaaaa
    22681 tcagcattgt ccaaatgaca aattttgtta ctttagcaaa agtacttgtt tgtttttttt
    22741 cacgcattgt tttcaacctc gtcattaatt agttgattca agtgtacaat cttttttcca
    22801 tttccacaag agggcttttt tataaaaact tccctttttt taaataatag gtagttacga
    22861 tacaaataaa taatgttaat agaactagcc atataaaggc atcatccctt tcagcaatag
    22921 gtaacttaat gttcattcca tagatactgc cgacgattgt cggtatagtt aaaataatcg
    22981 ttaaagaagt taaaattttc ataatattat tcagattatt cgatacgatt gctgaaaaag
    23041 tctgattcat ttgttctact agttttaatt gaatgcgaga agtagtcatt gcctgctctg
    23101 tttccaccaa tacatcacgt aaatgatttt tcaaagcatg attttcttgg aaaagtttat
    23161 tttgcgctag ttctttcaat gcatctaaat tagcagaagt cgcttctctg aaataaacga
    23221 ggcttttttg gatatccatg atttgataaa gttgtttgtt ttcagtggat acttgaagtt
    23281 tggcttccaa agtatcgcat tgagattgaa gcgtagctaa ataactgttg taagagagtg
    23341 tgacctgcca aatgaattga aagaagatat tgagattagg cgaaatatca ctttttggaa
    23401 aggtctggct aaagactttc tttaaaaaca gtggttcatg attcgtaatt gtgataagct
    23461 tctttttggg cgtcaaaata atagagagcg ggtatgtatc cacttgaaaa taaccgctag
    23521 gacttgtttt agcaaaagga tattgtaaca ataataacgc tggctttgtc aactttgatt
    23581 gattgaattc ttctgtacgg gaattttctg catcgtccaa tatggaagtg atgtaatctg
    23641 acggaatttc aaattgatgt gtcagctgat gaatctcttc ttgattagga cgttctacac
    23701 aataccagat cgtttggtca gtttcctttc cggtattacg cagtttctca ttatctaatg
    23761 taaaatattt gatcatgatt ttcctcctgt tgcctgtttc ttttattata acggataggg
    23821 aattggaata catggaatgt gtttgtaaga tatagaatat aactgcataa atttaaataa
    23881 aatgaaacag ttgtgtaaag tatatacagt atacttgaag caacgataaa aaaggagttt
    23941 acgttcatgg gcacattcac accacttatc ttgttttggt atatttaccc ggtaatcgtt
    24001 ttgtttggtt gccaatttct tgtaaaatta ttttcgttaa atcgtcgttt taaaataaaa
    24061 gcccctgatt tggcgattcc ttttttgtgg ggcggtatgc atgcattgtc tagaaataca
    24121 gggacaattt ctatcttacc gtttttcttt atttctgttt tgctaatggg aatttgtatt
    24181 gctgttttcc aagcatatta ttatgaagaa attatttatc cgcgttattt caagatgact
    24241 tggcgtttgg tttttctgtt gacaatcgtc ttgtacgctg tcttgatcat tgtgaacata
    24301 ctttcttacc tatgaaaaaa atagaaaagc tgtaaaaagt cttttaaaag gctttttgca
    24361 gcttgatttt taaaaaaatg tttatgaata aaaaaagttg ttcccggatt tcccagaaaa
    24421 ggtggtagaa agtgggggat tgtggtagac taattttagt tagtggagga atggggggct
    24481 ttcaatgttg atgggtgaat tccaacataa tattgatgca aaaggacgtt taatcgtgcc
    24541 ctcaaaactt cgtgaagaac ttggcgaaaa gtttgttctg acacgcggat tggatggctg
    24601 tcttttcggc tatccgatga gcgaatggga aaatctggaa gctaaactga acgaaatgcc
    24661 gttggctaaa aaagacgcac gtacttttgt gcgattcttc tattcggctg ccactgaatg
    24721 cgaacttgat aaacaaggac gaatcaatat acccagcacg ttaagaaatt atgcagcttt
    24781 gacaaaaggt tgcgtagtca tcggtgtatc taaccgaatt gaaatttggg atgagacacg
    24841 atggcaagat ttttctgcag cagcagaaga gaactttgac gaaattgctg aaagtatgat
    24901 tgatttcgga ctataagaat aggaagaggc tgactaatga ctgaaacatt tcaacactat
    24961 acagtcatgt tgaaagaaac ggtagatgga ttgaacatca aaccggatgg cgtttatgta
    25021 gactgtacac tcggcggtgc cggacatagt gaatatttat taagccaact aagttctaaa
    25081 gggcatctgt atgcgtttga ccaagatcaa aaagcaatcg atcacgccaa gctgcgttta
    25141 gcttcttatg tcgaacaagg tatggtcaca tttatcaaag cgaacttcag agaattagaa
    25201 gaacgcttga gtgagcatgt agataaggta gacggcattt tgtatgatct aggggtttcc
    25261 tctcctcagc tagatgaagc agaacgtgga tttagttatc atcaagatgc accgttggat
    25321 atgcgcatgg atcagtcagc tcctttatcc gcttatcacg taattaatga atattcctac
    25381 catgaattag taaagatttt cttccgctat ggcgaggaga aattttccaa acaaattgct
    25441 cgtgaaatcg aacgagtacg aaaaacaaaa ccaatccaaa caactggtga gcttgtcgaa
    25501 atcattaaac aagtcatccc agctcccgca agaagaaaag gcgggcatcc tgcaaaaaga
    25561 atttttcaag ctgtgcggat tgcagtaaat gacgaactcg gtgccgaaga ggcctcttta
    25621 gaacaggcaa tccggttatt gaaagtcaat ggaagaatca gcgtaatcac attccattca
    25681 ttggaagaca gaattgtcaa aagtatgttt aaagaatata gtacggtaca agatctacca
    25741 ccgggacttc ctgtcatacc tgacgaattc caaccagaac tgaaagtcat tactcgtaaa
    25801 ccaatccttc cttcagagga agagctaaca gaaaacaatc gatcacgcag tgcaaagtta
    25861 cgtattgcag aaaaaataag aatgaaagag gagtagaatg atggcagaat taaagaaaat
    25921 gcaagattac gaatatgatt tacctgaatt ggtggatcag ccggaacaac aaatgcctga
    25981 ttcttcttcg aatcaagaaa tcattcttcc acaatcaccg gcaaaacgat taaagcagat
    26041 ttctgctttg gaaaaaattg ctgtggcaag cgtggcgatt gctattgttg ctttatgtgt
    26101 tttaacagtt atgctgcgca caaatatcag cggagctgaa aaagaaatca cgtcaataca
    26161 aacagaaatc aatgataaaa agcaagaaaa aacgagttta gaacaagaaa aaaacgaact
    26221 ttctcgtact gaacggatta aacaaatcgc agaagaaaaa ggattgtcta ttaatgacga
    26281 caatttaagg aaagtgaagt aaatgagtct taaaaataaa attcggcggt ttatggaaaa
    26341 gaaaaatcta aatccgatga acaatcgcaa aaaagtaggt atcattttat ttgctacgag
    26401 tattggattg ttctttttat ttgccgtccg tttctcgtat atcgttattg gaggtcatgt
    26461 tgctggtacc tcattagctg aaaaaacaaa acagttgtac caaggaagtg aagtagtaaa
    26521 agcaaaaaga ggaacgattt atgatcgtaa cggtgtggcg ctcgcagaag atgcctcctc
    26581 ttattcaatt aaagcgatac tctcaaaaac atatacctct ggggataaaa aactgtatgt
    26641 tgaagaaaag aattttgata aaatagcaga aatcctgcat aagaatcttt ctatagacaa
    26701 aaaagatgca ctgaatattt tagaggacgg cgcaaaaaaa gagctgtatc aagttgaatt
    26761 tggtagctat gggaaaaata tctcccaaga aaccaagcaa aacatcgaag cggacatgaa
    26821 aaaagaaggt gtcgctggtc tttattttgt cgaccatcag gctcggatgt atccaaatgg
    26881 cgtcttttct tctcatttta ttggatacgc agttccagac aaagatgaga atggcttagt
    26941 tggtaaacta ggtttggagt ctgcttacaa tgatatcttg agcggtaaag atggtaagat
    27001 tatttatcaa aaagataatt ttcaaaatcc tctgccaggt acagtggcag aagaagaaaa
    27061 agctgtcgac ggacaagata tctacacgac tttagacagc agactacaaa gttatttaga
    27121 aacactgatg gatcaagtaa atgaagaata tcagccagaa gaattgacag cagtcctgat
    27181 gaaagcaaaa accggagaaa tcttggcaat gggacaacga cctactttca atcctgaaac
    27241 aatggaagga ttgactggaa aagatgctat ctggcgtaat ttcttggtac aagatagtta
    27301 tgaaccagga tcaaccatga aagttttcac tactgcagct gctatcgaag aaggagaatt
    27361 caacgagaat gaaaccttcc aatctggaaa aatccaagta gaagatgcta cgatcaatga
    27421 ccatgacttt ggtgaaaaag gtgtgctgac catgcgtcaa gctctttcat ggtcaagtaa
    27481 cgtcgggatg gtcatactgg aacagcgatt aggcggacga tggtataact atttacaaaa
    27541 actgggattt ggacaaagta ctcattctgg attagatgat gaagtaaacg gtgctttacc
    27601 tacattaaat attgtcgacc gagcaatgag tgcttatgga caagcggtag gtgtgacgaa
    27661 tttccaaatg atgaaagctt tcacttctat tgcgaataac ggtacaatga ttcaacctcg
    27721 atatatcagc aaagtggtgg atccccaaac tggagaagag cgaacaactc aaacagaagt
    27781 tctagggcaa cctttttcaa aagaaacaac agaaaaagtc cgtgaatata tgagagatgt
    27841 tgtcgaaagc gaaaactacg gtagtgctta tggtgtatat agtgtacctg gatacaatgt
    27901 atcggcaaaa aacggaacag cccaaatcgc ttctgataca gggggatatc aaacaggaga
    27961 tacagcctac ctgtattcga ttgtggagat ggttccttct gaagatccag actatgtctt
    28021 gtatctaaca atgaaacacc ctaagacata tgatcgaatg gcattggcta aaatcgcaaa
    28081 tccattgatg aaacgggcaa tggattttaa agaaactgaa gaagattcag atacagaaac
    28141 aaaaacagaa aaagtatccg tagccgatta tcgtaatctt gaagcggacg ttgcagcagc
    28201 tgatgctcaa aaaagtggac tgcaacctgt agtgatcggt aatggtaaaa aagtacaaaa
    28261 acaatctact gccaacggtg atcagttgat ctctggagaa aaactgatcc tatatactgg
    28321 tggtgataag ctgatgcctg acgtaacagg atggtcaaaa gcagatatca tgaagctggg
    28381 gaaaatcctc ggtattgaag ttagttttga tggagatggt tattgtgtca aacaagaact
    28441 tgctccatat gaaaaaatca ctgaagacaa acttagtttt actttagaag aataagaaga
    28501 gaaataggag agaaaagagc atggaatgga cacaagcatt gattcccatt gtcagcagtt
    28561 gtgcaatgac aattgccgca atgccgctat ttattggtta ttttcaaatg aaaaagcaag
    28621 gtcaggcgat tcgtgaggag ggaccaaagt ggcacaatag caaagccgga actcctacaa
    28681 tgggcggctt agtgtttctg atcggctcta ttttgacagg aatttgggtt ggagcttggc
    28741 aaaaacagct aactcctact cttttcatct tgctttttgt attagcgtta tacggagtaa
    28801 tcggctttct tgatgatttt ataaaaatat tcaagaaaag aaacatggga ctgaactcga
    28861 agcagaaatt gattggacag attctagggg gaatcatctt ttatcttgtt taccgttctg
    28921 aaggttatcc aggtgtattg aatttcttcg gactagatct acctttagga atcatttatg
    28981 gtattttcgc tctcttctgg cttgttggat tttcaaatgc agtaaatctg acagatggca
    29041 tcgatggatt agttgctggc ttaggaagta tctcttttgc agcttacggt ctaattgctt
    29101 ggcatcagca tcagtatgat gtattaatca tttgcttaag tgttttagga ggtttactgg
    29161 gtttcttcgt ctacaacaga aaacctgcca agatttttat gggagatgtc ggttcattag
    29221 cgttgggcgg acttttagcg gcaatttcta taatgctaaa ccaagaatgg acattgcttt
    29281 tgattggttt gatctatgtt atggaaacag caagtgtgat gcttcaagtt acttccttta
    29341 agcttacagg aaaacgaatt tttaaaatgt ctccaattca tcatcacttt gaaatgtgcg
    29401 gatggtcgga gtggaagatt gatacagttt tttggttgat cagcattgtg acttcgttga
    29461 tcacattatg gatcgtttgg taggagatga cgggaaatga aaaaaatatc aacatatgaa
    29521 aataagaaag tgcttgtctt aggactcgca aaaagcggag taagtgcggc caaattatta
    29581 catgaattgg gcgcacttgt caccgttaat gatggaaaac cttttgacga aaatccggaa
    29641 gcacaagagc tgctttcttt agggataaaa gtaataacag gtagtcatcc aatcgaattg
    29701 ttggatgaag aattttcatt gatggtcaaa aatccaggga ttccctacag tcatccttta
    29761 gtagcaaaag cccaagaaat gggtattccg gtaattacgg aagttgagct agcttacgaa
    29821 gtagcggaat gcccaattat tgggattaca ggaacgaacg gaaaaacaac gacaacaacg
    29881 atgacaggac tactactgaa tgcaggagca gatcaaggaa ttgcacgttt agcgggtaat
    29941 attgggtatc cagcaagtgg tgtagcgcaa gaagcaaaat cagaagataa aattgtgatg
    30001 gaattgtcta gcttccaact catgggaatc acagatttcc gccctcatat agcggtgatt
    30061 accaatatct atgaagcaca tattgattat catggtacaa gaaaagaata tgtcaaagct
    30121 aaatggaacc ttcaaaaaaa catgacagaa aaagattatc tgatccttaa ctggaatcag
    30181 tcagaactac aagaattagc gcaaagaaca aaagcacgcg tactgccttt ctcgacaaaa
    30241 gaagtgttgg aagacggtgt gtatgctgac gattactcaa tatactataa aaaagaaaaa
    30301 ataatggaaa tatctgaact tggcgtacca ggtaagcata atgtagaaaa tgcactagct
    30361 gctatttctg tagcaaaatt gtatggtata tcaaatgaag ctatacgaga aacgttgcat
    30421 tttttccatg gcgtaccaca tcgtacgcaa tatgtaggag aaatccaagg cagaaaattt
    30481 tataatgatt caaaagcaac caatatttta gcgactaaaa tggctctaag tggatttgaa
    30541 acgtctaagg tcgtgttatt ggctggcgga ttagacagag gaaatacatt tgatgaatta
    30601 attccttcat taaaaggaat caaagcaatg gtcgtatttg gccaaacaaa agaaaagttg
    30661 atggatgctg gaaaaaaagc aggcatcgag acaatagtaa cagccgattc tgtagaacaa
    30721 gcagttcctt tagcattaga aaacagtaca gatggtgatg tagttttatt atcaccagcg
    30781 aatgccagct gggatcaata cccaaatttt gaaactagag gaaacagatt catggaagct
    30841 gtgaaccgat taaaatagaa aaagtagaag cgagcgataa aaaatgaaaa ttttggttac
    30901 aggcggagga acagggggac atatttatcc agctcttgcc tttgtcaatt atgtaaagac
    30961 tcaagaaccg aatgctgaat tcatgtatgt gggagcaaaa cgaggtctag aaaacaaaat
    31021 cgtccctgat acaggaatgc ctttccatac cttggagatt caaggattta aacgaaaaat
    31081 ttccatgcat aacatcaaga cggtccgatt gtttcttaag agtatccgtc aagcaaaaaa
    31141 aatcctcagt gattttcaac cagatatcgt tatcggtact ggaggatatg tttccggagc
    31201 ggtagtgtac gctgcagcca aaatgggtat tccaacagtt atccatgaac aaaacagtgt
    31261 accaggtgtt accaataaat ttttaagccg atatgtggat cggatcgctc tttcatttaa
    31321 agatgcggct tccttttttc ctgaaaacaa agcagttctt gtaggtaatc ctcgagcaca
    31381 agaagtagca gatactaaaa aatcagatat tttgaaaaca tttgatttgg atccagaaaa
    31441 gaaaacagtc ttgatttttg gcggtagtca aggagcactt aaaatcaatc aagcagtatc
    31501 agttttttta agtgaatata ttccttcttc ttaccaagtt ttgtatgctt caggagatag
    31561 atattatgaa gagatcctaa ataaaataaa tcctgaaata caaaatatta gtgtccagcc
    31621 ttatatcaaa aatatggcag aggtcatggc ttccagtgat ctgctggtgg ggcgtgctgg
    31681 tgcaacttcg atcgcagaat tcacagctct ggggttaccg gcagtgttgg ttcccagccc
    31741 atatgttacg aatgatcatc agactaaaaa tgcaatgagt cttgttcacg caggagcagc
    31801 aaaaatgatt gcagataatg aactaacagg tgaatcacta agtcagacag tcaacgaaat
    31861 catgggtgat gaagaattgc aaaagcaaat gtgccgagct tctaaagaac aaggaatccc
    31921 tgatgcttca aaacgtcttt atgacttagt aaaacaaata atcaaaaaat aaaggcggtg
    31981 gtcttagtga gtaagaaaaa agaaccaaaa aaattgtctc atccgtcttc tgatgaaaac
    32041 aatttgactc cttggcaaaa ggctaaccga aaatatctgg cagaacaaga gatgaaaaac
    32101 caagagccaa aaaaagaaga cataacatta gatacacccc tttcagattc tgaagaagaa
    32161 aaaacggttc aagaggaaca gcaagaagaa agccagtccg agaaaaaaat cgaatttttt
    32221 gaagagatca agcaaaagaa aaacggtgga ccatataacg gttctttttt gaaccggctg
    32281 cccaatttaa aaagtcaacg gcacaaagta ctatttcgtc ggttagcttt gatcatcact
    32341 gtattgggta taccgctgat ttttttgatc tattatgttt ctccttatag caaattacaa
    32401 gcagttgcag tatcaggcaa taagacagtc aacagccaag aaatcatctc tgatacaaaa
    32461 ttgtcattag gagaaaatgt ttggggacag tattttcatc gatctactta tatcgatcgc
    32521 cttaaaaaag cacagccaag aatcgaaaca gcaaatatac atttcaaagg gatgaatgaa
    32581 ttcgatttgg atgtaacaga atataaagag attgctttga tcgcaaaaaa caatcaatac
    32641 tacccagtga ttgaaaatgg aacagtatta gatgaaaaag tagcaaatcc tacaaaaaat
    32701 ctccctatct tggaagagtt caaagactct gcaaaaatca aagaattaac aaagcaatac
    32761 aatcagttat catcagaatt acaaaaagca atttctgaaa tcaagtacac gccaagagca
    32821 agcaataaaa atctgatcca attgaatatg aatgacggca atcaagttat tgtaaatatc
    32881 aataatcttg cgaaccaaat gaaatattat tcgcaagtag ccaaagatat ggatgaaaaa
    32941 ggtgtgatcg acatggaagt tggtatcttc tcgcatcctt acggtagcac tcaagaaacg
    33001 gaagaatcgg gccaatcgga agaaacaact gaaagtgaag aaaatgcgga tcaaacaaac
    33061 gaatcaagtg aagaggcaga aacgcaaaaa gcacaaacac ctggatcgtc tagcacaaac
    33121 ggatcatctg attcaaatga ttcaagtgaa acaagtcaaa tagaagataa tccaagtaca
    33181 actccgtcaa gtttaaatga aaattgataa aacaacttta aaaagtaaga atgtactttc
    33241 taaaaaccag taatttatgg tagaattgga agcagtgaat atatctatta ataagtatat
    33301 tttaaaacgg acgctagttg ttcgcttgta tatggaatag taagtaggag ggaatcccat
    33361 tcatggcaaa aacaggaatg tatgtaggcc ttgatattgg aacgacatct gttaaagttg
    33421 tcgtggctga atatatcgac agccaaatga atattattgg agtaggaaac gcaaaatcag
    33481 aagggattaa ccgaggcatt atcgttgata tcgataaaac ggtccaagcg atacaacgag
    33541 cagtacgaca agcagaagaa aaagcaggta tccagatcaa aggcgtaagt gtcggtttgc
    33601 cagcaaacat gcttgaagta gaaaattgcc agggaatgat tgcagttaac ggagattcaa
    33661 aggaaatcac cgatgaagat gtcagaaatg tggcttcagc tgcccttgtt cgttcaatac
    33721 ctcctgaacg tcaaatcgtg tcaatattgc cacaagattt tactgtcgat gggttcgagg
    33781 gaatcaaaga tcctcgtgga atgatcggcg taagattaga aatgtacggt ctgttgttta
    33841 caggacctaa aacgatcatc cataacatac gtaaatgcgt ggaaaatgcc ggattaatcg
    33901 tcaacgaaat ggtgattact cctttagcgc tgactgaatc aattctttct gacggagaaa
    33961 aagattttgg tacgatcgtc attgatatgg gtggcggcca aacaacaacg gctgttatgc
    34021 atgataagca attgaagttt accaatcttg accaagaagg tggcgaattt gtcactaaag
    34081 atatctctat tgtattaaat acgtcattta acaatgcaga agcgttgaaa ataaactacg
    34141 gggatgcata cccagaacgg acatcaccag atgaagaatt tccagtagat gtgattggtc
    34201 agtcagaacc agtgaaagtc gatgaacgct acttgtcaga aattatttca gctcgaatgg
    34261 aacagatttt caacaaagca aaagatgcat tggaccaaat cgaagcatta gagttgccag
    34321 gtggcgtcgt attgacaggc ggagctgcta gtcttcctgg tgttgtggac ttggcacaag
    34381 aaatcttcgg cgtaaatgta aaattgtatg ttccaaatca catggggctg agaaacccgg
    34441 tatttacaaa tgtaataagt atcgtggatt attcagcaaa cttgagtgaa gtgtatcaat
    34501 tggcaaaaat tgctgttacg ggtgaaacct cagcagctcg tcaaatggta gttgaacaag
    34561 aaacaacaaa cacatatgag agctatgaag cacctgaaga aacaatctac gatgagccgg
    34621 aaccaaaaga gtccggagaa aatgtaaaaa ataaaatcaa gggtttcttt acaaatattt
    34681 ttgattaatt catcctggga ggaataagat atggaattct caattgataa taatatcaat
    34741 gacggcgcag taatcaaagt catcggagtc ggtggtggcg gtggaaatgc tgtaaaccga
    34801 atgattgaag aaaacgttaa aggtgttgaa ttcattacag ccaacacaga cgtgcaagca
    34861 ttaaaaaatt caaaagctga aacagttatc caattaggcc ccaaatatac tcgtggttta
    34921 ggtgctggtt ctcagccaga agttggacaa aaagcggcag aagaaagtga acaatcctta
    34981 cgcgaagctt tagacggtgc agatatgatt ttcatcactg caggaatggg tggaggaacc
    35041 ggtacaggtg ctgcaccaat cgttgcaggt atcgcgaaag aattgggcgc attgactgtt
    35101 ggtgtcgtga cacgtccatt cacttttgaa ggaccgaaac gtggccgttt tgctgcagaa
    35161 ggtatcgctc gtttgaaaga aaatgtggat acattattaa ttatctcaaa taaccgttta
    35221 ttagaagttg tagataaaaa gacaccgatg cttgaagctt tccgtgaagc agataatgtt
    35281 ttacgacaag gtgtacaagg tatctctgat ttgatcacag cgccaggtta cgttaacctt
    35341 gactttgctg acgtgaaaac agtgatggaa aaccaaggta cagcattgat ggggatcggt
    35401 gtggctagtg gagaagatcg cgtagtagaa gcaacgaaaa aagcaatctc ctctccattg
    35461 cttgaaacat ctattgatgg agctgaacaa gtacttctta acatcacagg cggtttggat
    35521 atgacattgt tcgaagcaca agatgcttca gatatcgtag caaatgcagc aactggcgat
    35581 gtaaatatca ttctaggtac atctatcaat gaagaaatgg gcgatgaaat ccgtgtgacg
    35641 gtcatcgcaa caggtatcga tgaatcaaaa aaggaacgca agtctagccg accagctaga
    35701 caagcacaaa tgcaatcacc tgctcaaaag actgtattag atatggatca agctaaacca
    35761 acttcttcag aagaagaaaa cagttttggc gattgggata tcagacgtga acaaaatgtt
    35821 cgtcctagag ttgatgattc caattttgac aatatcgaaa aaaaagaatt tgatacattc
    35881 aaccgcgaag aaacaaaatc tcatggcgat gatgaattaa atacgccacc gtttttccgt
    35941 cgtaaaaaat aaaggagagg aagagcacat gattgctgac aacttgcaga aaatcaagca
    36001 agaaattcag gattcgtgtg ctcttgttca tcgtgatccg aaagaagtta cattagtagc
    36061 tgtaacaaaa tctgttgaat ccgctcaagc tgaagaattg gttagagctg gcgttatgaa
    36121 ttgtgcagaa aaccgagtag ataaattatt agaaaagaaa caagatttat ttgattttcc
    36181 tgaaataaag tggcatttaa ttggtaattt acagcgcaga aaggtaaaat tgattgtaaa
    36241 tgaaattgat tactttcatg cgttggacag tttaagatta gcagaagaaa tccagaagcg
    36301 gttagagaaa gaattatcct gttttattga agtaaatgtt tctggtgagg aatcaaaaca
    36361 tggaatttca ccagatgagc ttttaccgtt tgtggaatct ttagctgcct gcacaaaaat
    36421 caaggttgtt gggttgatga ctatggcgcc taaagaagct tctgatgagc aaatccataa
    36481 agtgtttggt acgctgaaag aattgcgtga tcttgttcaa gcaaaaggtt atgcccatgc
    36541 accatgtagt gaattaagta tggggatgag tcaagacttt caaattgcga ttgaagaagg
    36601 tgcaacattt atccgtgtag gaacagcgct attcaaagac gaataaagga ggaacaaaaa
    36661 tgccgctttt aaacagagga gcaattgcta attttttcgg attagctgat gaggaagagt
    36721 acgaatacca agattatcct gaaacaacaa aaaaggctgt acagccaact aaaaaaaata
    36781 caacacagaa tcaatcaact cattctgtat ctgctgcgaa tactcatgaa aaacaaaaaa
    36841 cggcgacttc acgtcctgta acgcatgaaa gtattcagcc ggctgaaaag aaaaaagtaa
    36901 ttgaactaca tcaatcacct acaaaaactg tagcgaaaga aagaacagct tcaagaacct
    36961 ctacacaacc taaagcaaat cttgctggaa aaatcactgt aatggaacca agagcttatt
    37021 ctgaagctat ggcgattgct aaacgaatca ttgctggaga agcaacctta gtgaattttc
    37081 atttagtaga agaaaaacaa gcgagaagaa tcgttgattt tctaacagga acagtttatg
    37141 cactagatgg agatatcaaa cgtgttggag atgagatttt tctttgtaca ccgactggga
    37201 tggagattga cagttcaacc gctcaatctt ttgcagatgc gaatttgttt gatctgtaag
    37261 aggaggaact acgatttata ctattgcact attacttcat ttacttcaac gggcagccta
    37321 tttctatagc attatcttga tggtatatgc attgctctct tggtttccag gcgggtatca
    37381 atcagggcta ggacgattat tagctaagat ctgcgaaccg tatatcagtt tgttcgatcg
    37441 tctgcctttg aggattggac ccttggattt cacaatttta ggggcaatcc tgatcttgaa
    37501 cttagccgta caaggtttag cacagatcgt tatatggata actgtccatt tagtttattg
    37561 attgaagcaa caaaatcttt attctcagaa tagataggag gggaaaattt tggatgcaaa
    37621 tgtataccag cactttcggg cagatgaacg tacatttatc gattcggtag gcgattggat
    37681 tgaacaggtt caaggacaat atgcacccta tctgaccgaa tttcttgacc caagacaatc
    37741 ctatatttta gaaacactga ttcgacaaag cagtgatttg cgtttcatgt tttatggcgg
    37801 atatgaacag gcagaaagaa aaagatgttt gattttcccc gattattacg aacctgtaga
    37861 ggaagatttt gaaacagcat tgtatgaaat ccattatcct agcaaatttg ccagcctaag
    37921 tcatgggaaa atccttggaa ctttagtggg aacggggatc aaacgctctt actttggcga
    37981 tatcatctcg gacggtgatc gctggcaagt atttattgca aatgaggtgg atcattttgt
    38041 tgctgcacaa gtaacaaaga ttggaaaggt tgctgtacgc ttggaaccaa tcaaatacac
    38101 agaaatgatt atgccgaaag atagctggac acaagaaaga ggaacagtta gttccatgcg
    38161 actagacagt gtgatctctg agatttataa tatttccaga cagcggtcca agcagctgat
    38221 cgaagctggt aaagtaaaag tgaactggac agaaaataca agacctgatt ttgtacttga
    38281 actattagat attatttcag tcagaggtta tggcagagtc cagatccaag aacttgaggg
    38341 gaagacgaaa aaagaaaagt acagggtact gttcggtgta ttacgtaaat aaaataaaaa
    38401 ggtggtttac tcatggcttt aactccatta gatatccaaa ataagacatt cccgacaaaa
    38461 atgcggggat ataatcaaga cgaagtagat gatttcttag atttagttgt tcgtgactat
    38521 gaggaattga ctcaaagaaa tcgagaatta gaaaaagcag tcaaacattc tgaagaaaaa
    38581 cttgagtact tcaatgaatt aaaagatgct ttgaaccaat caatcatcgt agctcaagat
    38641 acggctgata aagtaaaaac aagtgcaagc aaggaatccg aagtgatcgt tacttctgca
    38701 caaaacaaag cagatgagtt agttgcgaac gctgaaaaac gtgcacatca attaacaact
    38761 gatgcagaag aaaaagcaag aaaaatcttg acagatgcaa cagaaaaagc aagacagctt
    38821 gcgacagaaa ctgaagatct taagaaaaaa acacgagtat tccatcaacg tatcagcttg
    38881 atgcttgagt ctcaattaga acaagtgaaa agtccggaat gggatgaaat tttacaacca
    38941 ttctccagct acgtgactga cagccatgaa gtgatcaaag aagttttagc aaaacagctt
    39001 gacaatgaaa atgatactga tgtaaactca gacacaggag tcatagaagc acctatgacg
    39061 gcatttgata cacaggctat tgatatctcg atcgtgcctg gtgaaaatga aaacgagtag
    39121 aacagaaaag cactttgtta ttcttaaagc gagccgatga gagtggaagt tcggtaagac
    39181 actgcatggt gaaagatcct ctacttgaga cctgcttttc cgaaattaga gtaagaagca
    39241 gcggctgacc tcgttattgg tcacagagag tttgcactag atagatgcaa ctaaatttgg
    39301 gtggtaccac ggcatgttcg tcccttgcgg ctgaacatgc ttttttgtgt ccggaaaaca
    39361 aataaaggag tgtatggttg aatgaaaatg aaagaaacct tgcaattagg aaaaacaaaa
    39421 ttccctatgc gtggtaattt gccaaaccga gaaggcgagt ggcaaaaaga atgggaagaa
    39481 aatcgaatct accaaaaaag acaagagttg aacgaaggaa aacctagctt tgttcttcat
    39541 gatggacctc catatgccaa tggtaatatc catttaggac attcattgaa taaaatcagt
    39601 aaagacatta tcattcgttc aaaatcaatg tctggctatc gtgcaccata tgttccagga
    39661 tgggatacac atggattgcc gattgaacaa gtattggcta ataaaggcgt gaaacgtaaa
    39721 gaaatgtcca tggcagaata cttggaaaaa tgtcgtgaat atgcactttc ccaagtagat
    39781 aagcaacgag cagatttcaa acgcctagga gttgcaggag attgggaaca tccttatgtt
    39841 acattagatc cttcttatga agctgcacaa gtacgcgtat ttggtaaaat ggctgaaaaa
    39901 ggctatattt acaaaggatt gaaaccaatt tactggtctc cgtcaagtga gtcttcttta
    39961 gcggaagctg aaattgaata taaagatgta aaatctccat ctatttttgt agctttcaat
    40021 gtcaaagacg gcaaagatat tcttgatgaa gatacagctt ttgtcatttg gacgacaaca
    40081 ccatggacat tgccagcaaa ccaaggtatt gctgtaaatc caaaatttac gtatgtattg
    40141 gtggaagccg atggccgtaa gtttgtcgta gcgaaagatt tgctagaaac agtccaatca
    40201 gaaatcggtt gggaaaatgt aacagttcta aaagaatttg ctggacaaga aatggaatat
    40261 atgactgcaa gtcatccatt ctatgaacgt gaatcacttg tgattttagg cgatcatgtt
    40321 actttagacg ctggtactgg attagtccat actgctccag gacatgggga agatgactat
    40381 atcgctggta gcaaatataa actatctgta gtgtcacctg tagactcaaa aggattgttt
    40441 acagaagaag cacctggatt tgaaggtatc ttttatgaca aagcaaatcc tatgatcact
    40501 gatctattaa aagaaaaagg agctttgctg aagctagatt tctttactca tagttatcct
    40561 cacgattggc ggacgaaaaa gcctgtgatc tatcgggcaa ctccacaatg gtttgcatct
    40621 atcgataaat tccgtcagaa tattttggat gaagtagaaa aagtagagtg ggttatccca
    40681 tggggtaaaa cacgtttgta taatatgatc cgtgaccgcg gcgattgggt aatttcccgt
    40741 cagcgtgcat ggggagtacc acttccgatt ttctatgctg aaaatggaga agcaatcatc
    40801 acaccagaaa caatcgaaca tgtggcaaaa ttatttgctg aacatggatc aattatctgg
    40861 tttgaaaaag aagcgaaaga acttcttcct gaaggattta cccacccagg ttctcctaat
    40921 ggcgaattta caaaagaaaa agatatcatg gatgtctggt ttgattctgg ttcttctcat
    40981 gaagctgttt tacgtcagcg accagaatta tcattcccag cagatatgta tttagaagga
    41041 tcagaccaat atcgtggctg gttcaactca agtatcacga ctagtgtggc tatcaatggt
    41101 gtagcaccat acaaatcagt tatttctcaa ggatttgctt tagatggtga aggccgtaag
    41161 atgagtaaat ctttaggaaa catcattgca ccagaaaaag tgatcaaaca atttggtgca
    41221 gatatccttc gcttatgggt atctagtgta gattatgaag cagatgttcg tgtttctatg
    41281 gatatcttaa gtcaagtggc agaagtatat cgtaaaatca gaaatacgat gcgtttctta
    41341 ttagcgaata cagaagattt cgatcctaaa aaagatactg tttcgtacaa tgatttacgt
    41401 tctgtagaca aatatatgac cgttcgattg aaccaagtaa tcaaagaaat ccgagaagaa
    41461 gggtacgaca aatataactt catgcatatt taccgtacag ttatgaactt cttgacggtt
    41521 gatctatcgt cattctacct ggattttgct aaagatgtag tttatattga agcagaagat
    41581 gattatcaac gtcgttgcat gcaaactgtt ttctatcaaa cagctgtagc tttgacaaaa
    41641 ttattgactc ctatcattcc gcatacagca gaagaaatct ggtcattcct gaaagaagaa
    41701 gaggagtacg ttcagttaag cgagtttcct ggttatgaag agtttgcaaa ccaagaagaa
    41761 ttgatggata cctggactgc tttcatggac ttccgtgata atgtcttaaa agcattggaa
    41821 gaagcaagaa acagcaaatt gattggtaaa tctttggaag caaaactaac tgtttatcca
    41881 aaacaacaag ttcgtgaaat gttaaaagct ttggatgcag atattgcaca attattgatc
    41941 gtttcaccag actactttga agttatggca gcagatgaac aagtgccaga taacgctatg
    42001 gtatttgatg atgttgctat tacagtggaa aaagcagacg gggaaacttg cgatcgctgt
    42061 cgccaagttc gtaaagatgt aggagtagat gaaaaacttc ctcatctatg cggtcgttgt
    42121 gcaaaaatcg ttgaagactt ctatcctgaa gctgtagctg aaggattcga agaaaagtaa
    42181 aagggaaaag ggggtgtgac atgttctttg tcacaccccc ttttgttatc aatcgtttaa
    42241 ttttccacgt tcacgatacc agttatctgg ttgccagatc cattcgaatc cgtctttttc
    42301 aagtagttcg aaagctgctt taggtcccat tgatccagct ttgtagttgg gaaaatccgg
    42361 ttgtgtttgg tcccaagctt ttcgaatgac atccacgata tgccaagatt gagcgacttc
    42421 ttcccaatga gagaagttcg ttccatcacc attcaacgca tctaatagta atttttcgta
    42481 agcttctggg ctattttcaa ccatttctgc actatttcta aattccaatt tgattggttc
    42541 cgtttcaaat ccttgcccta cttctttccc attcaaggat aaagaaaatc cttcagttgg
    42601 ctgaatatag atcgttagaa cattaggtgg aagtgtcgta tcgtcacatg gttcatcgac
    42661 agatgttttg aatacattaa caggaacttg tttgaatacg atgttgatcc gagtcccttt
    42721 ttcagtcaaa cgtttgcctg tacgtacata gaaaggaaca cctgaccaac ggaagttatc
    42781 gatagtgaat ttgcctgcga cgaatgtttc agtctctgat tgagcatcca ctttgtcttc
    42841 ttcgcgatag cccttgaatt gtacaccatc caattcacct tgagcatatt gcccgcgaac
    42901 gaagttttct aacacttctt cttcgttata aatgcggata gctttcaatg ctttgatctt
    42961 ttctgtacgg atttcttttt cggaaaatgc tactggcggt tccattgcaa gtaatgctac
    43021 aacctgtaaa atatgatttt gcaccatgtc tttaagtgcg ccactatgat cgtagtagcc
    43081 cccgcgatct tctacaccga ggttttcgcc aaaagtaatt tggatattgt cgatatagcg
    43141 attgttccat tgagcctcaa aaatattatt ggcaaaacgg atagctgaga tgttttggat
    43201 catttctttt cctaaataat gatcaatcct gaaaatatct tgttctggga atacttctct
    43261 gatttgctgg ttcaattcaa aagcagactg gtaatcagaa ccgaacggtt tttctatgat
    43321 caaacgattg aagccatttt ttgtcataat attttgtgat ttcaaatgag aaacgatcgt
    43381 tccaaaaaat tgaggtgcca tagccaaata atagacacgg ttaccgttta attgatagtg
    43441 tccatctaat cggtcagaca attcttttaa tgtctgataa tgcgttgaat catttacatc
    43501 atgggattga tagtaaaaat ggcttgcaaa ggtagttgct tcttcatctg aaggattcaa
    43561 tgcacggatt gtttctttga caacttcacg gtagtgctca tcgctccagg gacggcgagc
    43621 ggttccgatt acagcaaaat gctcgcctaa atttcctttt ctgtaaaggc ggaacaaaga
    43681 cgggtacaat ttacgctggg ctaggtcacc tgtaccgccg aatatcgtaa acaatacatt
    43741 tttttcatta gacatgtgga tacactcctt tatattttat cgacaaaaat cgtgctggcc
    43801 gccttaaagc tgacggggat tttctttcta tcattttgga tagtgatcgg tccttcataa
    43861 gctgcgatgt caacgatctc atattcttcg ttgatattca ggtcaatagt gactaagtaa
    43921 tccaacaatt ctttttcatc aagcactcgt gcgatacgca cttttgtacc gacaggatag
    43981 gactccaatg tttgtcggcg ttcttcatgg acaacttcct cttttttagg aatcgatcct
    44041 ccatgaggac aatgttcagg atgattcaaa tagttttcta aatgatctgc aagatgttcc
    44101 gaagtcacat gttccaatac ttcagcatca tcatggactt cattccagct atagtctaaa
    44161 tgttcaacta aaaaaacttc ccataagcga tgtttgcgaa tcagactgct agctttttgc
    44221 agtccgcttg cagtcaattg tacaccttga taaggggaat gttctacaag ctcttctttc
    44281 acaagtttag aaatcatttc actgacagat gcagctgaga catctagact agatacgatt
    44341 tgtttattgt tgactttcgt tgtatctcca cctagttcta aaatgatttt caaataatct
    44401 tctcgatttg gtgtcatatt cgatcatcct ttcatggtaa aaaaccatca ttagtaagtg
    44461 taacaaaaaa aacgttaatt gactattttt ctaaataaaa aacaaaggat ttttagacgt
    44521 ttttacaaca aaaaacgaaa gatgatagag aagactcatt ctttcgttta taaaggatat
    44581 atatatacat tgggagggat ctatttttca gataacaact tatatgaagt gtctgcagat
    44641 tcgtcataaa gagtttgttc gggataagaa aagataagat aaaaaccata ctgatcacgt
    44701 acgactaaac gatattcatc tatttctacc acagatgtgc caagagactg accgttgatc
    44761 aaaaacgtgt aatcagatgt aatctgtaat tgttgttttt gctggctatc actaaagaac
    44821 catgtacctt gatactttga gagtccttct gacttttccg cttgtttttt ctttttcaag
    44881 taaataaaac ttgagagaac ggtaatgagt gaaccaagaa aatagatcat tttttttatc
    44941 accaaatcac tcctttatga gaaaataatc gcatagacat attctttctt ctttatttca
    45001 ttgtaccgaa taaaagctaa aatttctcta agactttcga ctggtttttt tactgttttt
    45061 ctacaaatta tgataggata catagtagga agaaaggtga cataatttga agtatcagca
    45121 agtggaaata gcttttttta ttgaccgccc gaatcggttt atagcacatt gtatgaaaaa
    45181 agacggagaa ataattattg cgcatgtcaa aaatacaggt agaggaaaag aggtcctgat
    45241 accaggcgct gaagtggcta ttgtatttgc tcctggacct aaacggaaaa ccgcctatga
    45301 tctgattgct gtaaaaaaga agggtgactg gtttaatatc gatagtcaat tgcctaatcg
    45361 gctggcaatt gatggtattt tagacggcac gatcctttta ccaggattaa ctggtccatt
    45421 tacggtatac aagcgtgaag taacttatct tcactccaaa tttgatattt atttagagac
    45481 ggtggtcgga caaaaagcat ttgttgaagt caaaggtatg acacttgaaa ataaagcgat
    45541 tggtgctttt cctgatgcgc ccacactcag aggattgaaa catataggag aattaacgta
    45601 tgccgcacaa gatggttatg ccgcttatgt cttgtttatt gctcagttcg aacatcttca
    45661 tctggcaacg atccatgaag aaatgcaacc agcactagct gatatggttc gacatgcgca
    45721 gcaaagcgga gttcaaatcc tcgcctataa ttgtcaagta tcggcagatg aggtaactgt
    45781 caagcaggcc attccatttg atgtagatat cccttttgaa gatcctatac aatagaaaga
    45841 aggtaaaata atgatcaact taggtattat cggaacaaat tggattactc atcaatttgt
    45901 ccaagcagct ttaagcacaa aaaaatatga attgacagcg gtatattcta gacatttgga
    45961 aaaagcacag gaatttggtt ctcattatga aggggatatc gaattcgcaa cagatttgaa
    46021 gacttttttc aatattactc atatggacac catttatatt gcttctccga atagtcttca
    46081 ctttagtcaa gctaagcaag caattttggc aggaaaaaat gtgatagttg aaaagccagc
    46141 tttttcgacg ccaaaagaaa tggatgaaat catccatttg gcaaatgaac acaatgtgta
    46201 tttttttgaa gcagcaagaa atatccatga atcaggtttt aaaaagattg ccgattactt
    46261 accattaaaa gacgagataa tcggtgcaaa tttcagctat atgaaatatt cttcgcgcta
    46321 tgaccaagta ttagatggag aagaaccgaa tatcttctcg actcatttct caggtggtgc
    46381 attgatggat ttaggtgttt accttgtata tgcagctgtt gcttggtttg gaatgccaaa
    46441 agaagtccat tatttcccaa gaaagatttc tactggtgta gatggtcttg gatgggggat
    46501 cttacgttat gagaattttg atgtatcgat ccagccaggt aaaatcggtg attcttattt
    46561 accgtcagag atttattttg actcaggaac gctagtgatg aatggagtca atgcaattga
    46621 acaagcacaa tttcatgatc gcgcgcataa agaaatcatt gatttggcgg taacagctgc
    46681 agaaaatcca atggcagatg aagcagagga ttttgccaat gtcatggctc atccgaaaga
    46741 tccagcttgg ggattgttgt atgaagagtg gatcgagcta gctagaaatg tgaaccaagt
    46801 gatttatgat ttaagaaaaa atggcggcat caaattcgat gcagataatg aggaagattt
    46861 ttaatgaacg aatttgtaca agtatttccc gaaaattggc agaaaaaata tgaggataaa
    46921 gggtttatct caccttctct tattcaacga gcagtttttc agccactaag taataaacaa
    46981 agtatagtag cggtttctcc tacaggttca ggtaaaacac tggcttatct ttggcctcta
    47041 ctactaaacg tggaaccagg tgaagcaagt gcgttggtca tttttgcttc ttctcaagaa
    47101 ctagctattc aagtagcaga tgttgcacgt gaatggggaa aagacaaaga gttgaaagtc
    47161 caatctttgg tgggcggagc caacgtcaaa cgtcagattg agggtttgaa ggaaaaacca
    47221 gaaatcttga ttggtacacc aggaagaata ttggaattga tgaaagcaaa aaaaataaaa
    47281 gcacatcaag tgaaaacaat ggtatttgat gaagcagacc agctatttga cgctgggaac
    47341 agccaaatca ttgatcaaat tcttcatcaa gctccaacag agtatcagct agcttttttt
    47401 tcagcgacag cagatcgttc ccttgaaagc attgaaaaaa tcactgggaa aacgtttgag
    47461 acaattgacg tgacagcaga ggacgattct cgaaaaggac tgagacatta ctttttacgt
    47521 gtaccgatac gaaagaaaga tgactacttg agacgtctca cgcatataga agattttcat
    47581 ggtctgattt tctttaatca actgactgaa ttaggcgtaa tggaagaaaa attgctgtat
    47641 agaggtgtac cggtaggaag tctggcctct gatcagaaca agctactccg aaaagcagcg
    47701 ctagaacaat tcaaaaagaa aatggtcaga gcacttttga ccacagatgt ggcggctaga
    47761 ggtttagata ttacaggtct tccttatgta gtgaatgcag aagttccgtt aagtgaagaa
    47821 gcttatttgc atcgttcggg aagaactggt cgaatgggta atgaaggaat agtaattaca
    47881 ctcgtacaag ataataatct aagagattta aaacggcttt tacgtccatc aaatataaca
    47941 cttgaagaga tttttttata cggaggtaag ctgcaaactg agcagccaac caaagaagat
    48001 gcagctcctg cagtaaataa aaagtatgcg aaaacttttt ctggaaagga gcagaaccca
    48061 gaatataaca aagaaaaaat aagaaaaaaa gttaaaaata aatcaaaaaa agagagaaat
    48121 aaaggagcaa ggcgcaaata agcgcctttt tatgctatga ttatgaaaac gggaacaaaa
    48181 ttggttgcca gaggaggggt tatgtgaaag aacgtcaaga agagatcttg cagctattga
    48241 cagaaaacca acaaggattg acagcaagtg atgtagctga gcgcttaact atcgatcgaa
    48301 gcaatgcaag ccgatattta agtgaactat acaaggccca tcatattgta aaaaccgctg
    48361 gccgaccagt cgtttactct ttgccaacgg aaaagtcaaa atctgatgaa gtgcatgtcg
    48421 actcttctac gcaagtgact tttgaaacac tcgttggaga aaatgattct ttaaaagtca
    48481 gcatacagca ggcaaaagct gcgattcttt atccaccgcg ggggttgcat acgattattt
    48541 ttggagaaac tggtacaggg aagtcgatgt ttgctgaatg tatgtatcat tttgcaatcg
    48601 attcagagat gctttctgca gatgcgccat ttgtttcttt caattgtgcg gattatgctc
    48661 aaaatccgca actgctcttt ggacacatct ttgggatcaa aaaaggggct tacacaggag
    48721 cagctcaaga tagtcccggt ctaatagcca aagcagacgg cggtatattg tttttagatg
    48781 agatccaccg tttaccgccg gaagggcagg aaatgctgtt tactttcatt gataaaggta
    48841 tttaccgtcc gcttggagaa agtgcacaag tacatgaagc atctgttcag atcattgggg
    48901 cgacgactga atcttcagaa agtttcttat ctacgttcaa tcgacggatt cctatggcaa
    48961 tcaccttgcc gaatcttgct tcacgttcac tggatgagcg ttatgaaatc atctctcttt
    49021 ttatcaaaca agaagcgaat cgtttgaatc aacgtatcga tatcgaaaaa gaagcgattc
    49081 ttgccttcat gctttatgat gcggaaggaa atatcggtca agtgaaacga gacttgaaac
    49141 tagtctgtgc aaaatctttt ttacattacc gtactcatca tgagaaaaag ctaatcattc
    49201 gaaaagaaga actttccctt caagtacaaa aaggattact gaaaatcaag gaaataccgg
    49261 aacgtctcga ccgattcatg gacagcaaaa gtcagtattt gacatttgaa ccaggatttg
    49321 ctgatgttgt ctggtcacaa gatccagaac gaaacatgca ggtctataac gatattgagg
    49381 aaaaagtcac ttctctttcc gaaaccggtg tggagaacat tgatctagag accctcattt
    49441 caaaagatgt ggatgcttat ttccagacat atgtcaaaga attgactaaa agttcgattc
    49501 ccaaagatct tttgccagat gaaatctggc ggttaaccaa tcaattgtat gatgtggctg
    49561 aaaaacggtt agaccgtatt tataatgaaa aagctcgttt tgcttttgct ttgcatctgc
    49621 aaagtacatt ggatcgtgtg agagaaggtc atatgatcgt ccacccagat ttgaataacg
    49681 tacggaaaaa ttttaagagg gaattccagg tagcaatcga tctttcaacg atcattgaag
    49741 aagaagaaca agttgagatt ccttttgatg aaatcggctt tattagtatg tttctttcta
    49801 tcaatctagg agaaagtgag cctgttaaag aaaacaaagt agatgttctc atattgatgc
    49861 acggcagaca gactgcttca agcatgttgg agatggcaca agaactatta ggaacaaaag
    49921 tagggaaagc ttttaatatg ccactggtta tggaagttca aacaatgtat gaagaagtgt
    49981 tacaatatgt caaaaactat caagagcatt tgacgaatgg cctactcttg ttaacagata
    50041 tgggatcatt aaacaccttc agtgatttga tttatgaaga gactggcatt cggacgaaag
    50101 ctatcacgat gacaagtacc atgattgtga tagaagcgat acggatggca agtgttggaa
    50161 gaagcttaga agatatttat caaaatatcc agctttcctt tgaatcgatc gtacgtgatc
    50221 agttcaaagc ggaaaagtct gaaactttac agcgtaaaaa agccgtaatc gtgacatgct
    50281 ttactggtga aggagtagct gccaaattat atcagcgtat tatgccagtg attaatcaaa
    50341 ataaggtaga actgatccag atgcagtttt tagaacgtga atcattcaaa aaacacatcg
    50401 atgccttatt ggaagaatat gagatcaaag caattgcggg aacagttgat gttgattatc
    50461 aaaatatccc atttttttca gcctatgacg tttttgatga tgaaaaacta aatattttaa
    50521 agcgtatagc tgatgatgag atcccgacag ctaaaattgt cgattcactt gctggatcat
    50581 taagcgatgt cttttctatt gatgaactga tccgttcatt gcaaaagaca gttcagcaga
    50641 tccagaccga tttgtcgatt atcgtggaac ctggagtaga tactggaatc gttatccatt
    50701 tagccttcat gttagacgga ttgatcaaac aagaaagcca tcggcaattt gagaaactgt
    50761 ctattttctc aaaaatttat cgtttggaaa tggatatcgt tcgggcaaac ttgatggcac
    50821 tagaacgaaa atatcaggta aaaattccag aagatgatat cgcatttctt acacaaatgt
    50881 ttcttgaaaa taaaataaaa aaatgaacac actaatcata ctgtttacac actaaacatc
    50941 tgtgtgcgca ctataaaaaa gagctaaaaa gcatgaaaac gctatttcag ctcttttttt
    51001 tattggcatg cttcttgcgt tatataaaag tgtaaaaaaa taaaaggagt gttaaaaatg
    51061 gcaaaaaaaa caatcatgtt agtttgttca gcagggatga gtacgagttt attggtcaca
    51121 aaaatgcaaa aagcagcaga agaaaaaggg atggaagccg atatttttgc ggtctctgcc
    51181 tcagatgcag ataataattt agaatctaag aatgtcgatg tattattatt aggtcctcaa
    51241 gtacgtttca tgaaagctca atttgaacaa aaacttgcgc ctaaaggtat tccattagat
    51301 gtcatcaata tgcaagacta tggcatgatg aacggagaaa aagttcttgc acaagcagaa
    51361 aatctaatga aataaatcaa gtatgcagtt gggggtataa caacgtggaa gatcaacaaa
    51421 atctagaagc catcatgggg ttaatcatgt ttggcggtaa tgcaaagagc gatgcaatgg
    51481 aagcgattgc ggctgctaaa atcggtgatt ttgagttagc ggataggaaa atcgctgatg
    51541 cagaagaatc acttatacaa gcgcatcatg cacaaacaga catgttgaca caagaagcac
    51601 aaggaaatca tatgcaagtg acattattga ctgttcacag tcaagatcac ttaatgacat
    51661 ctattgcgtt tactgattta gcaaaagaaa tcatcgaact ttatcgtcgc gtatacagcg
    51721 aataaataca acttcttttt tgaacggaag aagaaaaatt tttgaacgga ttttttattt
    51781 tgttataagg agaactctct ttatataaaa aacttatagg aggaagtaaa aaatgaacgg
    51841 actatcaagt tggatggaga aatacatcct gccggttgcc gcaaaaatcg gagcgcaaaa
    51901 acatttagtt gctttgcgtg atgcttttat cggcatactg cccgcaacaa tggcgggatc
    51961 agtcgctgta atgatcaatg caattattcg tgatttaccg caacagttta acagtaccta
    52021 tgctgacagt ttagcagcag acaaaggaat ctttgccgta ttgaatgtca tcatcggaat
    52081 caatggtttt gtctggaacg gtacactagc aatcgccgga ttgatctttg ccttttcttg
    52141 ggggtataat cttgcccgag cttatggagt aaatgatcta gctgggggga tcgttggttt
    52201 agctaccttg attcaaggta tttcctttgg tccagcatta tcagcagcat tagatgtgaa
    52261 tgttccttca aatgtcgcag atgcaatcaa tagttctgag attggtgcaa cgattgcaga
    52321 tggcacactg tcagttggat tatggggttg gctaaaacta gatcatttga atggaaacgc
    52381 ttattttaca gttatgatca tgggagctat ttcagtcatt attttttgta aattgatgct
    52441 tgccaacatt acgatcaaaa tgcctgattc agtaccacca gcagtatcta aagcatttgc
    52501 agccatttta ccagcgacga tcgctttata cgtcattgct attatcaatt atatcgtagg
    52561 tcgtgtagct gatgggcaac tgatcattga tttggtgcaa aaatatattg cagaaccatt
    52621 cctaggatta tctcaagggt tgggagcagt attgatcgtc acagtatttg ttcaagtatt
    52681 ctggttcttt ggtattcatg gatcaaatgt attggcacca gtgctagagg gaatctgggg
    52741 acaagcacaa ctaatcaata ttgatatttt ccaaaaaggt tataacggaa aaactggtac
    52801 accagccgtg ttagatgcaa tcgaagatgg aaaagcttat atgtgggtcc gcggatcgtt
    52861 cgatgctttt gcatggtttg gcggatcagg cggaacgatc gtcttactga tagctatctt
    52921 gattttctct aaacgtgcag attatctaac agtagctaaa ttatccattg ggcctggtat
    52981 cttcaatatc aacgaaccag tgatgtttgg tctgccaatc gtattgaatg caatcatgtt
    53041 tatcccattc ttgattgctc cagtagtagc aacagcaatt ggatggacag ctacttatct
    53101 tggcctagtc gctccggtat cgcagcaagt cacatgggta gtaccgccgt tcttattgtc
    53161 attcttagca acaggagcgg attggcgggc accgatcgta acattagtat gtatggtcgt
    53221 gactttcttg atttgggcac catttgtcat ctctgcaaac aaaatggacc ctgccgcagg
    53281 cgaacaacaa taagatgaac gtgaggagcg ggacgcttta agtttcgttc ctcataatga
    53341 attttcgagg aggaactgaa atgattacaa ttgccagtgc aatgcaacaa gatgaaatca
    53401 aagaattgtt agaaggatac aatgaagaag gaatgacttt tacatttaaa gaaaaacaag
    53461 gaatcaagct gttctttgaa acaagtgcag aagatccgga agttgcggct aaaaaagcaa
    53521 aggaactaat caaagcacag ccttggggaa cagtccttta ttttcaatca acagctgttt
    53581 agaaaatggt ttaaaaagat aggatggttg agatgataaa aaacggttta tttttatgta
    53641 gtattggttc tttgatcgtg ttgtatagtt tgaatccaca ttcgcaaagg tatgatttgt
    53701 atagtatggt cacaggtctt tttctaacag gattaggaat ctatttcttc atgaagggga
    53761 aaaaaagaga agaagcaaag gagaaaaata actaatggaa atcacgaaaa aattaaatat
    53821 tccagcatcg tttttttacg ataaagttat ggactctgtc cttttcgatg tacgtaaagc
    53881 gaccggcaaa tcagtaacta gaaaacagct gaaaaatttg gagtacgtca aacaattctc
    53941 gcaaaataac cgggctcgca taaaaatcga agaagtcatt gaaaaccagt cttataagtt
    54001 tcgtacatcg acaacgaaga atgaattcgt cgttcattat caaatcaaac cattggacga
    54061 ttcgacctgt gaggttcatt acacagaaaa aatggagtct tatggcatgc ttcaaaagtt
    54121 gaatgatatg cttttaggaa caatcttgat gtattttaaa cggagacgtt tcaaaaaaat
    54181 gctagatatg atcgaagcaa cgtattaaat caatgaaaca tggtactatt tagttattga
    54241 aaaatgaagt aagctagaag gaatttttca taggaagata gatctttcct gcgaatcctc
    54301 actagctttt ttgtaggaag gggcagcaaa atgacaaaca aagtaagaga gctaggtgta
    54361 tcttcgattg aggaaatgtt tgatttagcc acttatgcat ttaatggggc agatacacct
    54421 gaaaggcgag aacgttttcg tacacttgcg gagaattctt ggaattacgg attttttgat
    54481 gaggaggatc ggctaacaag tcaagtcatg gcgacccctt ttccagtgaa tttttacgga
    54541 caagagtacc taatggccgg cattggctat gttgcttctt atccagaagc aaggggacaa
    54601 ggcgggatca atcagatcat ggaaaagatc cttgaagatt gccgtgatcg taaagtaagc
    54661 ttatcttatc tggcgccatt ctcctatccc ttctatcggc gctatggcta tgaacaatca
    54721 tttgataaaa tcagttatca actaaacagt cgtgatgtgc cgtttatcaa aaaaaaatct
    54781 ggaaaaataa agcgtatgga ttttgaagat gcaaaagctg caatcagaag aatttatgaa
    54841 cagatgccgg aaaaccaacg agctggtctg aagcgcgaag actggtggta tacgtataaa
    54901 ttcaagcaaa aaggcaatta ccagtttgct ttgcattttg atgataatga ggaagcaacg
    54961 ggatatatcg tttatcagct tgcaacgtct tcctttatta ttgcggagtg gggatttttg
    55021 gatcatgatg ctttttgcag ccttgttcgc ttcgtaagtt ctcacaatgg tgcttttgaa
    55081 acatttgaat attcatgcgg ctacggcggt aatgatcaaa attatctatt agaaaatcca
    55141 tttgcttctg taatgatcac accttatatg atggcgagga tcgtagatat ttctttgttt
    55201 ttggaaaatt atccatttaa aaagagacaa gctgcatttg ccctacaaat agaagaagat
    55261 cattatgcga aatggaatga aggaatcgtg gaagtaacaa tcgaaaatgg aaaaaatcat
    55321 ctctcaaaag tagaacaaac aaaactccca atcgttcgag gaagtatcca acatatcacg
    55381 cagctattat tagggtatcg taaagtggaa gagttggtat tccaagaaag aatacaggtg
    55441 acaaaagaat atgaagaaac gttaagctca atcttgccac agcaacagcc aattcttaat
    55501 gactattttt gataaaaaac gggtgatgac aatatttttg tcaccaccct tttgtttttc
    55561 atttggcatt tgaaggaaaa tgttaaagta aatcctcttt tttcgtaaat ttgaattttt
    55621 tgttttcttt tgttttaggg ttttcttttt tgtggtcttg gattgcttct ttcgtaatct
    55681 tttcccgatt ttttttagat gaatctaagc gttctgcgta tactttttct tttggcttat
    55741 cattcattgg aaaggacctc cctacattat ttcattctac ctctagttta tcatctgtta
    55801 aggactagtg gaaacgtttt gcctaactta taagctgatt tttttagcag ataagcttgc
    55861 aagtaaaaaa caaacaagct acaattgatt tattgaaatt gttcctgtag ctcagtagga
    55921 tagagcaatc gcctcctaag cgataggtcg ctggttcaat tccagtcagg aacgtataaa
    55981 taacccctgt atcctttgcg gtacaggggt ttgttttgta tttgtgccaa aaatgtgcca
    56041 aactagttat catttttata taaactatcg aacacagtat tgattttttc tttatcctga
    56101 tcttcaagct ctcgaacaat atgaagataa gtagacattg tagtttctaa actgctatgt
    56161 cctaaacgtt tcgatacgct aagtatattt actccttgat aaagtaaaat agatgcatgc
    56221 gtatgtctta gtccatcaat aaacagtcga agagaaaata gggaaaaatt gattactaaa
    56281 gaaaaactta ctaggtcgac ggtgtagttg attttcatac ttcaatagat tcgaattaaa
    56341 cgatttcgat tggcttatac cagtcctatc caacgaaaga tatatcgttc ttaactctag
    56401 cggtataact tgaagcttta cgtaaagcca atgggaatga actggagatg actttttatt
    56461 atttttagta atagctgatg ggttaatact tacaactttc cttttcaaag taccatataa
    56521 aaaaacggtg gggttgttta gcgaaataaa ggatctaagc caatagctta gatcctttat
    56581 tctatgcttt atat
//
LOCUS       DAYYCW010000007        56099 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000007 DAYYCW010000000
VERSION     DAYYCW010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.007"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..622
                     /locus_tag="ACHAYA_000411"
     CDS             134..622
                     /locus_tag="ACHAYA_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM8604243.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(1163..1834)
                     /locus_tag="ACHAYA_000412"
     CDS             complement(1163..1834)
                     /locus_tag="ACHAYA_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8604244.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(1831..2748)
                     /locus_tag="ACHAYA_000413"
     CDS             complement(1831..2748)
                     /locus_tag="ACHAYA_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8604245.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            complement(2745..3386)
                     /locus_tag="ACHAYA_000414"
     CDS             complement(2745..3386)
                     /locus_tag="ACHAYA_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8604246.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            complement(3390..4565)
                     /locus_tag="ACHAYA_000415"
     CDS             complement(3390..4565)
                     /locus_tag="ACHAYA_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8604247.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            4758..5789
                     /gene="queA"
                     /locus_tag="ACHAYA_000416"
     CDS             4758..5789
                     /gene="queA"
                     /locus_tag="ACHAYA_000416"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM8604248.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            6408..9047
                     /locus_tag="ACHAYA_000417"
     CDS             6408..9047
                     /locus_tag="ACHAYA_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8604249.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(9215..9913)
                     /locus_tag="ACHAYA_000418"
     CDS             complement(9215..9913)
                     /locus_tag="ACHAYA_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8604250.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            10131..11276
                     /gene="tgt"
                     /locus_tag="ACHAYA_000419"
     CDS             10131..11276
                     /gene="tgt"
                     /locus_tag="ACHAYA_000419"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM8604251.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            11373..11753
                     /gene="yajC"
                     /locus_tag="ACHAYA_000420"
     CDS             11373..11753
                     /gene="yajC"
                     /locus_tag="ACHAYA_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM8604252.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            12058..14655
                     /gene="adhE"
                     /locus_tag="ACHAYA_000421"
                     /gene_synonym="adhC"
     CDS             12058..14655
                     /gene="adhE"
                     /locus_tag="ACHAYA_000421"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM8604253.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            14884..15990
                     /locus_tag="ACHAYA_000422"
     CDS             14884..15990
                     /locus_tag="ACHAYA_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8604254.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            16007..17035
                     /locus_tag="ACHAYA_000423"
     CDS             16007..17035
                     /locus_tag="ACHAYA_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM8604255.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSNEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            17041..17520
                     /locus_tag="ACHAYA_000424"
     CDS             17041..17520
                     /locus_tag="ACHAYA_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM8604256.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            17536..18831
                     /locus_tag="ACHAYA_000425"
     CDS             17536..18831
                     /locus_tag="ACHAYA_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM8604257.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            18842..19666
                     /locus_tag="ACHAYA_000426"
     CDS             18842..19666
                     /locus_tag="ACHAYA_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8604258.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            19831..20730
                     /locus_tag="ACHAYA_000427"
     CDS             19831..20730
                     /locus_tag="ACHAYA_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8604259.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20856..21725)
                     /locus_tag="ACHAYA_000428"
     CDS             complement(20856..21725)
                     /locus_tag="ACHAYA_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8604260.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            21878..23338
                     /gene="rhaB"
                     /locus_tag="ACHAYA_000429"
     CDS             21878..23338
                     /gene="rhaB"
                     /locus_tag="ACHAYA_000429"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM8604261.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            23335..24591
                     /gene="rhaA"
                     /locus_tag="ACHAYA_000430"
     CDS             23335..24591
                     /gene="rhaA"
                     /locus_tag="ACHAYA_000430"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM8604262.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            24605..25450
                     /gene="rhaD"
                     /locus_tag="ACHAYA_000431"
     CDS             24605..25450
                     /gene="rhaD"
                     /locus_tag="ACHAYA_000431"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM8604263.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            25452..25763
                     /gene="rhaM"
                     /locus_tag="ACHAYA_000432"
     CDS             25452..25763
                     /gene="rhaM"
                     /locus_tag="ACHAYA_000432"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM8604264.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            25845..26996
                     /gene="fucO"
                     /locus_tag="ACHAYA_000433"
     CDS             25845..26996
                     /gene="fucO"
                     /locus_tag="ACHAYA_000433"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM8604265.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            27123..27881
                     /locus_tag="ACHAYA_000434"
     CDS             27123..27881
                     /locus_tag="ACHAYA_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM8604266.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            27981..28625
                     /locus_tag="ACHAYA_000435"
     CDS             27981..28625
                     /locus_tag="ACHAYA_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8604267.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            28632..29639
                     /locus_tag="ACHAYA_000436"
     CDS             28632..29639
                     /locus_tag="ACHAYA_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8604268.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            29655..30497
                     /gene="kduI"
                     /locus_tag="ACHAYA_000437"
     CDS             29655..30497
                     /gene="kduI"
                     /locus_tag="ACHAYA_000437"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM8604269.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            30517..31320
                     /locus_tag="ACHAYA_000438"
     CDS             30517..31320
                     /locus_tag="ACHAYA_000438"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM8604270.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(31569..32633)
                     /gene="fni"
                     /locus_tag="ACHAYA_000439"
     CDS             complement(31569..32633)
                     /gene="fni"
                     /locus_tag="ACHAYA_000439"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM8604271.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(32630..33715)
                     /locus_tag="ACHAYA_000440"
     CDS             complement(32630..33715)
                     /locus_tag="ACHAYA_000440"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM8604272.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(33728..34705)
                     /gene="mvaD"
                     /locus_tag="ACHAYA_000441"
     CDS             complement(33728..34705)
                     /gene="mvaD"
                     /locus_tag="ACHAYA_000441"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM8604273.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(34698..35642)
                     /gene="mvk"
                     /locus_tag="ACHAYA_000442"
     CDS             complement(34698..35642)
                     /gene="mvk"
                     /locus_tag="ACHAYA_000442"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM8604274.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            35958..36617
                     /locus_tag="ACHAYA_000443"
     CDS             35958..36617
                     /locus_tag="ACHAYA_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8604275.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            36702..37607
                     /locus_tag="ACHAYA_000444"
     CDS             36702..37607
                     /locus_tag="ACHAYA_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8604276.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(37609..38241)
                     /locus_tag="ACHAYA_000445"
     CDS             complement(37609..38241)
                     /locus_tag="ACHAYA_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8604277.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    38392..38517
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            38561..39085
                     /gene="infC"
                     /locus_tag="ACHAYA_000446"
     CDS             38561..39085
                     /gene="infC"
                     /locus_tag="ACHAYA_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM8604278.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            39157..39357
                     /gene="rpmI"
                     /locus_tag="ACHAYA_000447"
     CDS             39157..39357
                     /gene="rpmI"
                     /locus_tag="ACHAYA_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM8604279.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            39410..39769
                     /gene="rplT"
                     /locus_tag="ACHAYA_000448"
     CDS             39410..39769
                     /gene="rplT"
                     /locus_tag="ACHAYA_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM8604280.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            40021..41358
                     /locus_tag="ACHAYA_000449"
     CDS             40021..41358
                     /locus_tag="ACHAYA_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM8604281.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            41406..43535
                     /locus_tag="ACHAYA_000450"
     CDS             41406..43535
                     /locus_tag="ACHAYA_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM8604282.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            43510..44199
                     /locus_tag="ACHAYA_000451"
     CDS             43510..44199
                     /locus_tag="ACHAYA_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604283.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(44607..45284)
                     /locus_tag="ACHAYA_000452"
     CDS             complement(44607..45284)
                     /locus_tag="ACHAYA_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8604284.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(45420..45614)
                     /locus_tag="ACHAYA_000453"
     CDS             complement(45420..45614)
                     /locus_tag="ACHAYA_000453"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604285.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(45664..46104)
                     /locus_tag="ACHAYA_000454"
     CDS             complement(45664..46104)
                     /locus_tag="ACHAYA_000454"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM8604286.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(46108..46953)
                     /locus_tag="ACHAYA_000455"
     CDS             complement(46108..46953)
                     /locus_tag="ACHAYA_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM8604287.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            47102..48520
                     /gene="pepV"
                     /locus_tag="ACHAYA_000456"
     CDS             47102..48520
                     /gene="pepV"
                     /locus_tag="ACHAYA_000456"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM8604288.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(48577..49524)
                     /locus_tag="ACHAYA_000457"
     CDS             complement(48577..49524)
                     /locus_tag="ACHAYA_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8604289.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(49747..50097)
                     /locus_tag="ACHAYA_000458"
     CDS             complement(49747..50097)
                     /locus_tag="ACHAYA_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8604290.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            50297..51376
                     /gene="pepA"
                     /locus_tag="ACHAYA_000459"
     CDS             50297..51376
                     /gene="pepA"
                     /locus_tag="ACHAYA_000459"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM8604291.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            51520..51840
                     /locus_tag="ACHAYA_000460"
     CDS             51520..51840
                     /locus_tag="ACHAYA_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8604292.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            51862..52326
                     /locus_tag="ACHAYA_000461"
     CDS             51862..52326
                     /locus_tag="ACHAYA_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8604293.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            52531..53136
                     /gene="ytpR"
                     /locus_tag="ACHAYA_000462"
     CDS             52531..53136
                     /gene="ytpR"
                     /locus_tag="ACHAYA_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM8604294.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(53175..54464)
                     /locus_tag="ACHAYA_000463"
     CDS             complement(53175..54464)
                     /locus_tag="ACHAYA_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM8604295.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            54892..55371
                     /gene="rlmH"
                     /locus_tag="ACHAYA_000464"
     CDS             54892..55371
                     /gene="rlmH"
                     /locus_tag="ACHAYA_000464"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM8604296.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            55574..55699
                     /locus_tag="ACHAYA_000465"
     CDS             55574..55699
                     /locus_tag="ACHAYA_000465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604297.1"
                     /translation="MIDEKKCLKNLLEDMDDIEENYLNGLGKQIYFRYLSYLKMK"
     gene            55696..>56099
                     /locus_tag="ACHAYA_000466"
     CDS             55696..>56099
                     /locus_tag="ACHAYA_000466"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8604298.1"
                     /translation="MRHSNFLAFLFAPNETHNLSDEFFKMFLKRYIDSNDDTKAAINY
                     FDALLNSYEDLIVYRENKNIDILLVSEKNKIVVCIENKILSSESGGQLNKYQIYIERN
                     YSNYKKIFVFLTPDGNEPTNPSWGIITYKNIV"
BASE COUNT        17751 a        10277 c        11993 g        16078 t
ORIGIN      
        1 ttctaaaacc aaccttcttg gatttattgc atgatttctt taatttgttc ctatatctcc
       61 acctttgaag ggtcattgtt tgagtttagg aatggaatgt tgacgggttt atgtaaaaca
      121 aggaggaaga agtgtgacaa ggaaaatcag taaaattgga gcatcgttgt tagtgttggc
      181 agtggcaacg accatcgatt atcgccaaag tgaggcgaag acaattggaa atgtcgttta
      241 tcgagaaaca gccaaaagta tgttgaaaaa agcaatggac aatcaaccgg agtcgtctta
      301 ttggttccca gaggattcgt tggcatggtc ttttggaaaa gatacagata cgaaatacaa
      361 taccagtgtc gttcctttag cagagcgggt atccaaagcg acattgccga acatgaatgt
      421 cactcaaacg gaagaagtta aagtcgtggc gctttcggcc atgaatagca gtatcaacgg
      481 caatgcatca cgagggatcg atatctttta cgccaatgtc ttttcttatt ggcaatatat
      541 cgatcaattg gtttacgggg gtgctcttct ggtgaaggat catcgtatcg ccaagtctag
      601 ttgcgacgga tgcagtccat aagaatcgtg taccggtttt aggaaccgca aacctcccat
      661 ggcgagaagc tggaatggct agatatgttt ttagccaagg atgaggatgg caagttcccg
      721 attgtataaa agatgatcga agttaccgat acggcagtca ccagttttga tgatgcagct
      781 gaaggcatcg aacaatcgag gactgatgga aaaggcttaa atgaaagcca cgccaaagga
      841 tgtaagaatt gatgcccaat tcaaagaaca agcagacgag tatttagaga tcatgtggta
      901 tgaattccat gacgagtgat gggaaaatgg attggcaaaa tgccttagcg gataagaaaa
      961 aaacttagtc ggtggatgca gatatgaacc cgttggcagt atgaagttgg agtatacgga
     1021 atggataaat agctaagaaa agcaactctt tgcccataag gagggaagaa tcagagtgga
     1081 ttattgcctt aaaaatgaca gaactaaaaa agcctttgcg accaacaaat ggtcgcagaa
     1141 gcttttgttt tttacaataa catcagcgtg atgtacggct ggctggatct agccgtcttt
     1201 ccaaaatacc taaaagccag tccgtgatga tcgccattaa tgcagttggc aatgcacctg
     1261 ctaaaataat cgatgtgccc tctgttgcat tggttcctcg gataatgata tctccaagac
     1321 caccagcacc gacgaatgca ccgatcgcgg ttatcccgat ggcaacaact aacgcgtttc
     1381 ttatgcctgc catgataaca gaaacagaga gtggtaactc taccatgaat agtcgttgtc
     1441 tagcagtcat ccccatgcct ttgccgacat caaggatgtt tttgtctact tggatcatcc
     1501 cggtatacgt attcttgatg atcggtaaga gggaatacaa aaatacagta acaatcacga
     1561 cattgactcc caggcctaat ccaatgatta aaatggagat catggctaga gaagggatcg
     1621 tctggatgat attcgccaaa cgaatcaccc agtttgctaa tttacgtctc ctagaaatca
     1681 tgatcccaac cggaatcccg acaactgctg caaagaggac accatagata gaaatcagga
     1741 agtgacggat aaattgtgca aagatatagg agccattttc ctggaaataa tagaaaaatt
     1801 gttgaaagac attcatttta tccaattgct tcacttccct tcaaaatagt ggttttcttg
     1861 taagaaacgt tgtgctacta ctgatggttc cactagatta ttatctgctt cgtagttcaa
     1921 ttcctgcatt ttttccgtac tgatggtatg acctaactta tttaaggctt cttttaaatg
     1981 aggcgtatca gctaatatct tgttgtctac aactggagca gcatcatatg gtgggaagaa
     2041 ttgacgatca tcttccaaca tgactagatc ataactggcg atccgtccat ccgtagagta
     2101 gccaagaaca atatccattt ttcctgcttc gactgcgtca taaacaagtc caatctgcat
     2161 aggtaatatc gaattgaagg agaagccata agtttcttgg aagccgtcat aaccgtctcc
     2221 tttacgattg atccaagaag tatctacacc agctactaat tcatccgcta catttttcag
     2281 atcgctgact ttagatagat ggtatttttg tgcggtgtct tttcgaacaa gaaagacata
     2341 cgtattttca aaactatagg acgggaacca cgtttgaccg tatcttttct caaattcatc
     2401 tttcacggta gcaaacgctt ttttcggatc tttttcagga ggaaggttca atgtagtcgt
     2461 taaatctgtt cctgtgtagc gagccgctga aattgaagca tctccgttca tcatcgcttg
     2521 atggttgata gttgtagtag ccagattgtt gatgattgcg gtatttttat ctgtataatg
     2581 ttcaaccatt ccggcaacta gacttgctaa gatctgtgct tctgaagtga ttccacctgt
     2641 gatcgaaatg gtgtcttcat ctgtattgct ggcaagacca gggaaggaac aactcgcaag
     2701 tataagaaga gagaaaagaa ccatggtcat ttttagtata cgtttcattc ttcattctcc
     2761 tctctaagtg cgatcggtgt cagccggtgt tccaagagac ctagaagcca atcagccaat
     2821 agtgctaaaa ttgttacagg gatcgtacct gccaaaatga gatctggttg gtaattattc
     2881 agtccactaa aaatcaaatc gcctaaacca ccagcgccga tataagaggc aagtgtagcc
     2941 catgcaatca cgtaaacggc agctaaacga ataccagcca tgatcgttgg catagcaatg
     3001 ggcaattcaa cggaaaaaat ggattggaac tcagtcatcc ccatgccttt tgccacatcc
     3061 cgataattcc aatcgacatt tttcatccca atgtacgtat tacgtaaaat cggcaataat
     3121 gaataaataa agagtgcgat aatcgcaggt aatttaccta cgccaaatag cgggatcatc
     3181 aatgccaata aagcaagaga agggattgtc tgcaaggcac tggttaagcc aatcacaatc
     3241 gtggcagctt ttggaaaacg tgtcagtaag acaccaagcg gtacagcaat gatgatacca
     3301 atgatcaaag caagtcctga aatataaaga tgttcaaaac cttttgagag aatttctgaa
     3361 ccgtgctgat gaaaaaactg ctgcataggt cagacctcct tagattcttc aggagtagct
     3421 gactctgcaa catcagtagt tgcgggatct tctccccaaa tcacatcgta gacgatatcc
     3481 actaatgatg cacgagtcag gatacctacg actttgttct gttcatcaac gaccggcacg
     3541 tatttcagcc cgcgttttaa aatgcgttgc aaggtatcac gaagataggc ggattctttt
     3601 acgtaaaaaa catgtttatt caaaatgtcg ccgacactgg atacttttcc tcgtttttta
     3661 ttcaacgttt ctacatcgat aaatcctttt aatacattcg aattatcggt aactaacaac
     3721 gtatctacac gtttttccct catcagtttg attgcttctt gtaatgattt ttccggtgta
     3781 atcgtgattg ctgtatttag cataacttcg ccgactcttg tcgcatttgg cttggcttgt
     3841 agtaggcgat cttctccaat caactcttct acgaaatcgt ttgcaggatg acgtaaaata
     3901 ttttctggtg tatcaaattg gatcacacgg ccttcactca tgatggcgat acggcttgct
     3961 agtttaatcg cttcatccat gtcatgggtt acaaatacga tcgtttttcc caaacgctct
     4021 tgtaaatctt tgacaaggtc ttgcaaggaa tcacgagtga ttggatcaag cgcaccgaat
     4081 ggctcgtcca tcaagatgat atcttgattc gctgcaaggg cacgaacgac tccaatacgc
     4141 tgctgctgac caccagataa ttcatgtggg tagcgatcta acatctcacg gggcaattcc
     4201 acaagatcaa tcattttttc agctgtttta ttccgttctt cttgactgac tttcaacaat
     4261 ttttggacaa gagtgatatt ttcacggatc gtcatgtgag gcatcaatcc aatactttgg
     4321 ataacatagc cgatctgacg tcgcaattcc actggatcaa tttcttggat ggattttcca
     4381 tcaatcaaaa ttttcccttg tgttggatca gtcattcggt tgatcatccg catcgtcgtc
     4441 gtttttccgc ttccgctcgt accgataaaa caaatgaact cacccttttc aaatgaaaga
     4501 ttgacgtcct ctacagcgac ttttccacct ttataaattt ttgacacatt ttgaaattca
     4561 atcatggtgc cacctctttt tctattttca atgaagaact atctattact agtatgccta
     4621 aaacaatgaa aaaatacaaa tagggtagtt tttttattta ggaaaacaaa aaatagaagt
     4681 gaaaaagtgc tgattttgcc aacagaacga aaaacatgat agaatgatga agcacatgat
     4741 agaaagtgag tgacgatatg ttaacaacag aagattttga ttttgacttg ccggaagaat
     4801 taatcgcaca aacaccatta aaagaccgcg atcattctcg tttacttgtt ttggatcgga
     4861 aaactggaga gatggaagat aaacatttcc atgaaatcat tgaagagtta cagcctggtg
     4921 atgcgctagt tatgaataat acacgagttc tgcctgctcg tttatatgga gaaaaaccag
     4981 atactggtgc gcatttagaa gtccttcttc taacgaatac acaaggagat acgtgggaga
     5041 cattgatcaa acctgcgaaa cgtgccaaag ttgggacaag aatctctttt ggtgatggca
     5101 gattgcaagc agtagtgaaa gaagaattgg aacacggcgg acggatcatt gactttgagt
     5161 atgaaggaat ttttctagag attcttgaat cattgggaga aatgccattg cctccatata
     5221 tcaaagaacg tctggaagac ccagatcgct atcaaactgt ctatgcgaaa gaaaatggtt
     5281 ctgctgcagc gccaacagct ggattgcatt ttacgaaaga attacttgat gagatcgaag
     5341 caaagggtgt tgaattagtc tatctgacac tacatgtagg gttaggtaca ttccgacctg
     5401 tcagcgtaga taaaattgaa gaacaccata tgcatagtga attctaccgt ttgacggaag
     5461 aagcggctga gcgattgaac caagtacgta aaaatggcgg gaaaatcgtg gctgtcggaa
     5521 caacttcaat tcgaacatta gaaacgattg gaacgaaatt taatggagaa atcaaagcag
     5581 acagcggatg gactgatatt ttcattacgc ctggctatga attcaaagtg gtagaagctt
     5641 tctcaactaa tttccatttg ccaaaatcga ctttagtcat gttagttagt gcctttgcag
     5701 gaagagaatt gactttatct gcttaccagc atgcgattga agagcgctat cgtttcttta
     5761 gttttggaga tgcgatgttc gtgaaataag gttatgaaaa gccgctagtt ttgaataaca
     5821 aaaaagcttt acaaagattt tatgacaaag aaaaagccga gctctcgata agctcggctt
     5881 tcttgtacct cggaaggatt caaataaaca gaaatgattg taactttcca tcatacatgc
     5941 aaagcacggt gttgaatttc ttcatgttaa acaaaaattt tgttttcgtt acatcttcaa
     6001 catttgaaac atctcttgtt cctattcaat tttcagacgt ttacgttatt caattgttac
     6061 gtatcgcatt acttcttgtt tcggatactt cgtgatccaa acatccatac gcattcacta
     6121 tttataggtt tgtactctac acgcatgtct gggtgaaacc tgtacttcta acaactttgt
     6181 ttgcttacac tgttctatcg agaaattctt ctgtctattt gatggtatcc tccttcctta
     6241 catctatata atagcatcct ttggtagcgt ttgcaaatat aagcacttta aattttacgc
     6301 tttcttaaca aataatcgat aagcccttta tttgaagtaa ataaaaaaag gatgttatag
     6361 taaaataagt accttttttt gtacgaagat tttaaaggag gaataggatg gaagaataca
     6421 agcagacaat agaagaagta aagaaggtaa cagaagcaga tccagatact gggttgtctt
     6481 cacaagaagt ggatgaacgg cgaaaaaagc agggattgaa taaattcgat gaggcgccga
     6541 aagaatcgat gatcaaaaaa ttctttcgaa gcttgtccga ttttacaacg atcatccttc
     6601 ttgtagcagc ggtgatttcc ttttataccg catttgctac agaacatggg gatttatttg
     6661 aaggactgct gatcatcgct atcgttgtga tcaactcggt attggcgatt gtccaagaag
     6721 gaaacgcaga aaaagcactg gaatccttgc aagatatgaa taaacaaacg gcaactgtca
     6781 tacgagacgg caaagtgacc actgttgagt cagagcaact ggtagtgggt gatattcttg
     6841 ttttagagtc cggtgatgct atttctgccg atgcccgttt gatcgaagca tcacaattac
     6901 gtgtagaaga atcggcgttg actggagaaa gtgaagcagt tgaaaaagat gcagcattta
     6961 ttgctgaaga agatgagtca ttaggtgacc aacttaatat ggtatttaaa ggatgtacgg
     7021 ttgctgcagg tcgtgggaaa gcgatcgtca ctgccattgg catggctaca gaaatgggta
     7081 agatcgcgga tcttttgaac gaaaatacta tgcaaaaaac acctttacag gtccgattga
     7141 atcagctagg caaacgaatc agtatgatcg ctttagcggc tgcggcttta gtatttgtga
     7201 ttggtgaact gcaaggagaa ccactccttg agatgttcat gacttcgatt tccttggctg
     7261 ttgcagctgt accggaaacc ttgactgtta ttgttacgtt gacacttgca tacggtgtgc
     7321 agaagatggc gaggaaacat gcgatcatcc gacagttgcc agcagttgaa acgctaggaa
     7381 ccgctaatgt tatctgctca gacaaaacag gaacgctgac acaaaatgaa atgcgcgtcc
     7441 gccgggtatg gagcaaagaa gatgaagtaa cgaatatcga agattcaatg acgaacagcg
     7501 cgatggaaat attgaaaatg gctgcactat gtacagatgt gacggtcgag caagaagatg
     7561 acgaccttgt tatcaaaggt aatccaacag aagtagccat cgtaagggct gttgaagaga
     7621 attatcatac aaaggcagaa ttagaagaaa aatatccacg agtaaacgag cttccttttg
     7681 attctgaacg aaaaatgatg acaacagttc accaaatggg cgaaaaatac atctcaatca
     7741 ccaaaggagc attcgatgtt cttgctccgc gctttagttc aggcgatgta gaacaagcgg
     7801 cgatcgttaa tgatcgcttt ggtaaacggg cactgagagt gattgctgtt ggttatgcta
     7861 cttatgacga agaaccgcaa gatatttctt ctgaagcact ggaaaaagac ttacgtttga
     7921 ttggtctgat cggaatgatc gatccaccac gtccagaaag taaaggagca atcaagcgag
     7981 cgaaaaaggc tggcatcaaa acagtcatga tcactggaga tcatgtggtg actgccagtg
     8041 ccatagcgaa agaattaggt atcttgaatg atccgtcaga agcactttca ggatcagaac
     8101 tgcatcaatt atcagacgaa gaattagatg cgcgtgtcaa agctctctct gtctatgctc
     8161 gggtaacgcc agaagacaag atccggatcg tcaaatcttg gcaacgtacg ggtgcagtcg
     8221 tagcgatgac tggggatggt gtgaatgatg caccagcgtt gaaagcaagt aacgttgggt
     8281 gtgccatggg gatcacagga acagatgtgg cgcaaagtgc cgcagatatg attttgacgg
     8341 atgacaactt tgcgacgatc gtagacgctg tcagtcaagg acgttcggtt tatcagaata
     8401 ttcgtaaagc tatcaacttt ttgctaagct gtaatatttc ggagatcttt attgttttga
     8461 ttgcgatgct gttaggatgg ggagcaccat ttacagctgt gcaactactg tttgtcaatg
     8521 tagttgctga tggacttcca ggatttgctt taggaagaga gccagcggaa cacggcatca
     8581 tggatcagcc acccattccg aaaaatgaag ggatctttgc tcgaggttta ttgcaaaaaa
     8641 ttgccataaa tgcaggggtc ttcaccatcg taaccttgtt tggttattat ttaggtagtt
     8701 atgtcgatac tatcagccca tgggtagacg caagtcagca tgtcggtcaa actgttgcat
     8761 tcttgatctt agcttattca tcgattctgc atgtgttcaa tgtacgcagc agccaatcga
     8821 tcttcaaagt caatttagcg accaataaag ctttggtaga aatggcgtta ctggcattag
     8881 ctattacaac ggctgttgct ttgctgccat ttacgcaaga actattcggc ctagtacaca
     8941 tcagtttgaa tcactggttc ttggtaggga ttttatctat cgtaccaatc gcggtgaatg
     9001 aactgatcaa gttccatcga ttaccggaag cagaagaaga ggaatgatta actgtttcta
     9061 agttgatttt ctttttactg gaactataca aaaaggaggt gtgacatgaa tgttgtcaca
     9121 cctccttttt gctaatgggc tgaatgtttc ttttactgcc tctcgataaa cggtgttctc
     9181 ctgccacccc ctcggtcaca agtcacactc tcttttaaat atcttttggc aattgttcat
     9241 aaaatgctgc ttttgttgca gaaatgtatg ggctgagcca tagataacca ataccagcag
     9301 ttgctaatgc taagatatgc cagccgataa atgaaagatc taaccagaaa agttgtcctt
     9361 tataaccatc catcaatttt cgacttgcag tgatcgtatc cagtacttta ggtgtttgtc
     9421 ctgtttcttg gacgatatcg tagtaaataa aatagctttg agaataagaa tagcttttaa
     9481 tgattccagg aataactaat agcaaacccc ataatgttgt aaagatcgag atcagcacag
     9541 ctaagctgat cactccgccg ataaacaaac ctttgaatcc acgaaatgca tctttgaatg
     9601 gttcgatctg atctttttgt ccacgtaaaa tatcaagata agtccaagaa ataccgctca
     9661 tgaacaaagc gctaatgatg ctggaaataa ttccgccacc gcctcctgaa ctaccgcctc
     9721 ctgaagttgt cgcttctgag acgctcgctg atccatcata tatggtggtg gcaaacaaga
     9781 taataggaat cataataaga agaatagtag caataccaat caatgtaggg attagattta
     9841 gtaaaacagc ttgtccccaa cgtcctttta gactctcttt tgcttggcgt tttaattctg
     9901 cagaagattg catgattttt ccctcctttt ctttaataac accaatatac catgtatcaa
     9961 aaaatatttc tgtcataatg tttttctttt ttttgttatt cataaaaaaa ggataaaaaa
    10021 aagaatatta aataaagtag aaggaagccg agggaacttg caaaaagcac ttgaaacgat
    10081 tatgatggtg ggagttgtca agatatttta tatagaaagt agggaaatga atgacgcaac
    10141 ctgccattcg ctatcgttta atcaagaaag aaaaacatac tggcgcgcgt ttaggggaat
    10201 tgatcacgcc acacggaact ttccccacac caatgttcat gccagtagga acattagcta
    10261 cagtaaaaac aatgtcccca gaagagttga aagaaatggg tgctggcgtt attttaagta
    10321 atacatatca tttatggcta cgccctggtg aagacttagt agaagaagct ggcggactgc
    10381 ataaatttat gaactgggat caaccgattt taacagattc aggcggcttt caagtttttt
    10441 cattaagtga tatgcggaat atcgaagaag aaggcgtgca tttcaaaaat cacttgaacg
    10501 gttcgcgtat gtttttatca ccagaaaaag caatcaacat ccaaaataaa ttgggctctg
    10561 atatcatgat gagttttgac gaatgtccac cattcgatga aagttacgac tacgtgaaaa
    10621 aatcagtaga acgtacgtct cgatgggcag aacgaggatt gaaagctcat gctaatccag
    10681 accgtcaagg attatttgga atcatccaag gagcagggtt tgaagatctt cgtcgtcaaa
    10741 gcgcgaaaga tctagtcggg atggatttcc ctggttactc aatcggcggg ttatctgtcg
    10801 gggaacctaa atcagaaatg aatcgtgtat tagaattcac tacaccgttg atccccgaaa
    10861 acaaaccacg ttacttgatg ggtgtcggtg ctgctgattc attgatcgat ggtgttatcc
    10921 gtggagtaga catgtttgac tgtgtcttgc ctactcgaat cgctcgaaat ggcacatgta
    10981 tgacttctca aggccgtcta gtagtgaaaa atgcgaaata tgcccatgat ttccgtccaa
    11041 ttgatgagaa atgtgattgt tatacatgta aaaactatac acgtgcctat atccgccatt
    11101 tgatcaaatg tgatgaaaca tttgggattc gtttgacttc ttaccacaat ttatacttct
    11161 tgttgaatct aatgaaacaa gtacgtcaag caatcatgga tgataatttg cttgaattta
    11221 gagaagcctt ttttgaagaa tacgggttca ataaagaaaa cgcaaaaagc ttctaaagga
    11281 aaatctttta aaaattaagt aaaaaactag tgttatcagc atttttctgc taaagtgttt
    11341 tttgtagata cagaattagt gaggtgaaaa aaatgggaac attaccaatg cttgtgatgt
    11401 ttatcgcgct attagcaatg tggtttttta tgtcacgttc gcaaaagaaa caacagcaag
    11461 aacgtcaaaa cttgttagat agcatgaaac caggtgattc agtcgtaacg atcggcggat
    11521 tacatggagt tgtttcagaa atcgacaatg acaaacggac agttgtttta gactgcgaag
    11581 ggatcttcct tgagtacgat cgtgcagcaa tcaaaacggt caaaccaggg actgtcgtaa
    11641 caaatgatgc aacgactact gttgaaacaa cagaagaacc agctgagaca gttgaagtaa
    11701 aaactgaaac aacagaaacg ctggaagaga cagaagagaa ggaagaaaaa taagtatcaa
    11761 agcttattta aagacgaggt agggcagaaa gatgtcctac ctcatttttt attcaaaaaa
    11821 gaaagagata agagcatggg atatattgct caatattgaa taatcgggtg tgtcatataa
    11881 ataataataa ataactttat atatttttct atacaaacac ctgtaaataa agcaatttta
    11941 tatttaaaaa taactgtgaa aaaaatcaca aaaaagtgtt tacatagaaa aataaatatt
    12001 gtatgatata catgtgaatt atttaacaaa taaccatgac ccattaggag gataatgatg
    12061 gctaaagtaa tagaaaaaga aaaaacgaag acaattgacg tacaagcaat gattgatgat
    12121 ttggcagaaa aagcaaatgc agcattgaaa gaaatggaaa gctttgatca agaaaaagta
    12181 gaccatatcg tgcatgaaat ggcaatggca gctttggatc aacatatgcc attagcgaag
    12241 atggctgtag aggaaacagg ccgcggtgtt tacgaagata aagcaatcaa aaatatgtat
    12301 gcgtccgaat atatttggca taatatcaaa catgataaaa cagttggtgt gatcaatgaa
    12361 gatgtccaaa aagggttgat tgaaattgct gaaccagtcg gtgtggtttg cggagtaact
    12421 ccaacaacga atccgacttc cacaacgatt ttcaaatcaa tgatcgcgtt gaaaacaaga
    12481 aatccaatcg tttttgcttt ccatccaagt gcccaaaaat cttctgcaga agcagcacga
    12541 gtggtacgtg atgctgctat cgctgccgga gctcctgaaa attgtatcca atggatcgaa
    12601 catccttcga tcgaagcgac ttctatgcta atgaaccatc caggtatcgc gatcgtctta
    12661 gcgacaggtg gagctggaat ggtcaagtct gcttactcta caggaaaacc agctttaggt
    12721 gtaggtccag gtaacgtacc agcttatatt gaaaaaacag caaaaattaa acgtgcagtg
    12781 aacgatttga tcgtttcgaa aacatttgat aatggaatga tttgtgcttc tgaacaagct
    12841 gtgatcgtgg ataaagaaat ctatgcagca gtaaaagcag aattccaagc acaccaagtt
    12901 tatatcgtaa aaccagatga attgcaaaaa ttagaagatg cagtgatgaa cgaaggaaaa
    12961 tatgcggtca atccatcaat cgttggacat tctgcgatgg aaatcgcaaa attggcagga
    13021 atcagtgtac caaaaggaac gaaaatgctg attgctgaat tggaaggcgt aggcccagat
    13081 tatccgctat caagagaaaa actttctcca gttcttgcca tgatcaaagc taacaacacg
    13141 gaccatgcgt ttgatttgtg tgaaggtatg ttgaatcttg gaggattagg acatacagca
    13201 gttatccatt cagaaaacga agagttgcat gtcaaattcg gtcttcgaat gaaagcttgt
    13261 cgtattttag tgaatacacc atctgcagaa ggcggcatcg gtgatatcta taacgaaatg
    13321 attccgtcat tgactttggg atgcggttct tatggtaaga actctgtttc tcgtaacgtc
    13381 tctgctgtaa acttaatcaa tgtcaaaact gtagcgaaac ggagaaataa catgcaatgg
    13441 ttcaaattac cgccaaaaat cttctttgaa aagaactctc tattgtactt agagaaaatg
    13501 gaaaacgttg aacgtgtcat gatcgtttgt gaccctggta tggtccaatt cggttactgc
    13561 gatacagttc gtgaagtatt ggcacgacgt aaaaatgatg tgaagatcga agtcttctca
    13621 gatgttgaac cgaacccatc gacaaatacc gtatacgcag gaacgaaact gatggcagac
    13681 ttcaagccag atacagtaat cgctcttgga ggcggttctg caatggacgc agctaaggga
    13741 atgtggatgt tctatgaaca cccagacaca tctttctttg gtgcaaaaca aaaattcttg
    13801 gatatccgta aacggacata taagatcgac aaaccagaaa aaacacaatt cgtatgtatc
    13861 ccgacaacat cagggactgg ttctgaagta acgccatttg cggtcatcac tgacagtgaa
    13921 acacatgtga aatatccgtt agcagattat gcattgacgc cagatgtcgc aatcgtcgat
    13981 cctcaattcg tcatgtcagt tccagcatct gtcacagcag ataccgggat ggatgtgtta
    14041 acacatgcaa tcgaatccta tgtttcagtc atggcttcag attatacacg tggattgagc
    14101 ctacaagcaa tcaagttagt ctttgactat ttagaaaaat cagtaaaaac acctgatatg
    14161 gaatcacgtg aaaaaatgca caacgcttct acaatggctg gtatggcgtt tgctaacgca
    14221 ttcctaggta tttgtcactc agtcgcacat aaaatcggcg gggaatacgg gatccctcat
    14281 ggacggacaa atgcgattct tttaccgcat attatccgct ataatgcaaa agatccacaa
    14341 aaacatgcga tgttccctaa atatgactat ttccgtgcag atacagatta cgcagatatc
    14401 gcaaaattct taggtctgaa aggcgaaaca acagaggaat tagtcgaagc tttagcaacg
    14461 gctgtttatg aattaggaaa atctgtcggc atcaacatga gtctaaaagc tcaaggagtc
    14521 acacaagaaa cactagatac aaccgtagac cgtatggctg aattagctta cgaagaccaa
    14581 tgtacgacag ctaatccgaa agaaccattg atctctgaat tgaaacagat catcattgat
    14641 gcttacaacg gataagcaaa agaaagcgta gaaaaaagat aagaaaatag atgaaaaaga
    14701 acttggaaaa cgttgtttct ccaagttctt tttttaattt ccaagtaact gaaagttact
    14761 tttgttattt aacatttagg tcaattttag acagtttttg ttcaaaaagc gatatttata
    14821 gtggacggta ttactgctat attaaggata taaaatgaaa gcgtttaaaa aggtggcgta
    14881 aagatgatta ttaaagaaga gatttttgta cctaaagaat tagtgaaaga aaaaattgac
    14941 ctgctcgttg aaaacctgac aacgatcaaa gacgataatg gtgaattttt gttggacttt
    15001 gatggactga aagtagatga caaaagctgg acagtgtgga attggccaca aggtgtcggt
    15061 ctatatggta tttataaaaa ttaccagatg acaaaaaatc ctcgcgctta tcaagtggtc
    15121 aatgaatggt ttgaggatcg tatggaagaa ggcgctccgc ccaaaaacgt caatacaatg
    15181 gcaccgctat tgactatggc ttacttgtat gaagacacga aagattcacg ctatttgcct
    15241 tatttagaac aatgggcaga atgggtcatg aatgacatgc ctcggacaaa tgaagacggg
    15301 ttacagcatg ccacatatgg accggaaaat aagaatcagt tgtgggacga tacattgatg
    15361 atgacagtgc ttcctttggc aaaaatcggg aaactgttga atcgcccaga atatttagaa
    15421 gaagctcgtt accagtttat gatccatatc aaatacttga tggataaacg aacaggattg
    15481 tggtatcacg gctggacgtt tgaaggaaat cataactatg ccgaagcatt ttgggctcgt
    15541 ggaaactgct ggttgacgat tgctattcca gagatcattg aaatcttaga attatcgaaa
    15601 gaagatgcat tgcgcaaatt attgatcgaa acacttgaag cacaagtgcg tgcattaaaa
    15661 acttatcagt ctgaaagcgg tttatggcat acgttgttgg atgatccttc ttcttacgta
    15721 gaatcttctg caactgcagg atttgcttac ggtattttga aagcggtcca taaacgttat
    15781 ttaccacaag aatataaaga agtcgcttat cgtgctatcc aaggattgct tgaacaaatc
    15841 gatgaaaaag gagaagttca aaacgtttca atcgggacag gaatggggga cagtttagat
    15901 ttttatcgga atatcggaat cacagcaatg ccttatggac agtcgttaac ggtgctttgt
    15961 ttgacagaat tgctcgtctc ttattgttaa aagaaaggaa acccatatga aaaaaacatc
    16021 actgctgttt gcagcaagtt cgcttagcct aggtctgcta ggtctaagcg gctgcggaaa
    16081 taattctagt gtggcaactg tcgcagatgc agatgtaaat gagatagtta ccttgcgctt
    16141 tgcttatgca agcaacagcc agccagtaat tgatgcaatg aatgaatttg ggcgtttagt
    16201 aaaagaaaaa acagatggtt ccgtacagat ccagtatttc ccagatggac aactgggtgg
    16261 agaacgtgaa ctgatcgaat tgacacagtc aggagcagta gatttcacaa aagtaagtgc
    16321 ctcagcgttg gaaagtttct ctaaagatta ttcgattttt tcagttcctt atctattcaa
    16381 tgatgaagaa catttcttta aggtgatgga taatgaagaa ataatggagc caatctacca
    16441 atcgaccaaa aatttaggat ttaccggatt gacctattat gattctggtc agcgcagctt
    16501 ttatatggta gacggtccga tccatacgcc ggaagatttg aagggaaaga agatccgtgt
    16561 gatgcaaagc gaaaccgcta tcaaaatggt agagctttta ggcggttcgc ccgtacctat
    16621 gggaagtaat gaagtgtaca cgtctcttca atccaatttg atcaacggat cagaaaacaa
    16681 tgaatttgtc ttgtatacag ctggacatgg tggtgtggct aaatactatt cctatgatga
    16741 gcatacacgt gtgccagata tcatcatcat gaatgatgcc atcaaagaac agctaactga
    16801 tgagcaggaa aaagccattg aagaagcagc gaaagaatca acggtttttg aaattgaagc
    16861 ttttgcaaaa gcaattgaag aagaaaaagc tttagcacaa gaagaacatg gagtccaatt
    16921 caatgatgta gataatacac cgtttcgaga ggcagttgcc ccacttcatg atatattcaa
    16981 aaacgatcct acctacaaag acctttatca aaagatccaa gagcaaggag cgtagctgtg
    17041 atgaaaatga ttttaaacaa ggtattagag atcctgggag ctgttttgct tgttgggatg
    17101 gtcgctgttg ttttatggca agtattttcc agaacggttt taggaaatcc aaatacggtc
    17161 acggaagagc ttgttcgatt cggtcttgtc tggtttgcca tgttgtcttc cgcgtatgtt
    17221 gtgggacaaa aagcacattt agccgtaacg gttttaagtg aaaaattaac tggcaaaaaa
    17281 agtaaaattt tagaagttgt tgttcaaagt ttatttctgg tatttgctgc gatgatcatg
    17341 gtgtatggcg gatggaatgc tgtcacattg acaatggggc agatctcgcc ttctttaagt
    17401 attcctatgg gatatgttta tctgtcagta cctgtatcgg gggtactcat cattatctat
    17461 agcctgatca atctaatcga tacattaagc aacaagccag caattcaaga aaaaatataa
    17521 aaatggagga ataaaatggc aatagaagcc ggattagtgt tattttttgt atttgttgtg
    17581 ttgcttgtat tagggatgcc gattgctata agtattgcat tctcatcgat gatgacttta
    17641 ttgttagtta taccgtttga tgtttctgct tttagttcag ctcaaaaaat ggtgggaagt
    17701 atcaatagtt tttcgctgat cgctattccg tttttcgttc tctcgggcat tatcatgaat
    17761 aatggaggga tagctaagaa actagtggat tttgcgatgt tgttcgtcgg gcgcgtgccg
    17821 ggggctttgg ctcatacgaa tgtgttaggg aatgctttat ttggttccat ctctagctcg
    17881 gccattgccg cttctaccgc gattggcgga gtgttgatcc ctcagcaagt ggaagaagga
    17941 tatgaccgga agtttgcgac agcggtcaat atcgcgtcag ctccgacagg tatggtcatt
    18001 cctccaagta ctgcttttat catgtattca cttgttgctg gtggtgcttc gatctctgct
    18061 ttgtttatgg gaggttatct cgtaggcgct ttgtgggcgc taggaatcat gctagtagca
    18121 ttcatcgttg ctaaaaagaa taaataccct gttgtcgaaa aaggacagat ggctcatgct
    18181 ggaaaaataa taaaagaggc gattcctagc cttctattga ttgtcatcat tatcggtggt
    18241 atcttgacgg ggatatttac agcagtagaa gcttcagcga tagctgtcat ttactccctg
    18301 ttgattgcta tgttttacta taaaacggta aaactaaaag atatacctaa tatgttgttt
    18361 caatcagtag caatgtcagg gacgatcatg ttcttattgg ctacttcttc catgatgtct
    18421 ttcgccatgg cgtttaccgg tattcctcag gcaattagtt cgttgattat gggcgtaacg
    18481 gataataaat ttattattct gttattagtc aatcttgtgt tattactggt gggtatgttt
    18541 atggatgtgg cgcctgctat cttgattttt acaccaatat ttttaccaat cgtcacaagt
    18601 gtaggggttg atcctgtaca ttacgggttg ttttcaatca tgaatctgtg tgtaggctcc
    18661 atcacgccgc cagtaggtac aggactatat gtcggtgcaa gtgtaggagg cgtaaaagca
    18721 gaggcaatgc tgaaaccgct tcttcctttt tatggggtta tcttagtcgt cttgtttttg
    18781 attacttact tcccgcaaat cgtcatgtgg ctacccaata tgctgagtta ggagaatagt
    18841 tatgttgaac attacaatta gaggacatga tctttccgaa gtccaaacga tagaagatct
    18901 tgcagaaaaa acaaaagaac aagggattca tacactgcag ctcgccttag gtctttcgtt
    18961 tcctgaaata ccttctggcg caaatgagat caattctggg atgggaaatt atgtcaaacg
    19021 agttttagaa aagcaagaag tgaccgtagg aattttaagt tgctatatca atatgattca
    19081 tccagattta acgatacgac aagagttgct gacaaagttc gagcagtatc ttcgctatgc
    19141 ttcttccttt ggtgcgtcga tggttgcttc ggaaactgga tgtgttttgc cggaaatcca
    19201 atatacggaa gaaaatttca cagatgaagc atttgctgaa gctgtgtccg ttatccgtcg
    19261 cttggtaaaa gctggagaaa aataccaaat gatggtgggg atagaaccag gtttgaatca
    19321 tccggtgtat tctttagcaa gggtagagca gctgatccaa gcagtagatt cggactatct
    19381 aggaatcatt ttagatccga ccaatttgat cacaagtagt gattatcaag aacaagtcca
    19441 attggtagaa gaagcttttg aacgttttgg tgaaaagata tgtgcggttc atttgaaaga
    19501 ttttcgtgtc gaacaggaaa aaatcgtgcc ggtaaatttg ggagacggtg tgatcgagta
    19561 cacaaaaatc aaagaaatca tcatgaagaa tcgtccgtac ctgtatgtgg ttcttgaaga
    19621 aacaaaagat gatgggattc gttatggaag aagtcttctt gaataatgta aagaagacgt
    19681 tggttaacta atatttagaa tggagggggc ggggctagaa gatgccacgt ctcttctttt
    19741 ttataagagt atataaaagc agctcttttt gattacaaaa aacgtggtat aataaaattg
    19801 tgcataagtg aacaaaagga ggtgcggcac atgctccaaa agaaaatcag cggtgaacaa
    19861 gtcaatacga tggtttctga tcagtatgaa atctatcatg tcaaagatgt cgtcagccaa
    19921 aataaaacga tctatcatca tcatgatttc tatgagatcc atgctacatt aaagggagaa
    19981 gcgacttttt atttgaacgg acgacaattt acgatcaaag caggaagtgt gctgctcatc
    20041 cattccaatg atttgcatcg tatcgtccgc caatcttcag atgtattcga gcgtgtctat
    20101 atctttgtca ccccttcttt tctagaaagt cgttccacgc catggtctaa tctatcggct
    20161 tgttttcaac caatgggaga gaagaggagt cgtgtattgc aaatagaaca ggaagtattg
    20221 aagcaaaaat tatcatttat cgaccatcct cctgataaaa aaatttacgg tgaagacata
    20281 aaatatgaac aagctctggt agaatacttg atttttctca atcaagttgt acaaaaagaa
    20341 gagagtgcgc aagaacagaa tgctgtagtt cctaatgagc gtgtggaaca aatgttacaa
    20401 tatatctcaa aaaatttatc tcatccgctt tctttagagg aaatggaaaa gcagtttttt
    20461 gtcagcaaat accatatcac acgagaattt aaaaagtaca ctggatttac gtttcatcag
    20521 tatgtaatga agaaaaagct gatctatgcc aagcacttgt tgcgagaata ccgcagctct
    20581 agtgctgtat atagtaaaag tggtttttct tcttatccgc attttctgaa agcattcaag
    20641 agagagttcg gcataacgcc taaagaattt ttgaaacgtg atacacagaa acagttggtt
    20701 cattatgaac attttgaaga tgaacaatga gaatgatcac taaaaaataa ccgcagtagc
    20761 tgctgttgtt tacatagaag cagaaactgc ggttatttta tttttatcta tatatttcaa
    20821 aatattttga aaaaatgacg tacatattat atgaatcagc tgaatttacg aaagtctctc
    20881 ggcgatagtc ctgtttttgc tttgaacagg cggctaaagt aagcaaaatt ctcaatccct
    20941 atttcttcgg aaatcattcg aaccgtaaat tctgtatacc gaagaaggtc tttagcgtaa
    21001 gaaagttttt tgctgatcaa ataatcgatt ggtggagaac cgataaattt ggaaaaatct
    21061 ttgctcagct gatatttatt caataagaat tgttgttcta attgatcaag tgtaatcgtt
    21121 tctttgaaat gttcgtctaa gtattgcttc agtttgtaaa cgtaggcggg aatatcctct
    21181 tcttcgaaat ctagctgata cttttgcaaa atcagttcat tcagcaactc gtggatagct
    21241 acagaggact ggaaatcggt acgggcattc gtgccttgtt gcatgtggat caattctgtg
    21301 atcaaatggt ggatcttgtt ttctgatgga tctcctgttg aatggaaggt gttatggccg
    21361 tcttttgtga actcctgata aaatgattgg acgttctgac cattcaaatg gatccaatcc
    21421 atttcccatg gttcttcgct gatcgtttga taacgctgat attcttggca gtcaataaaa
    21481 aataagtctc cagcttttag ctgttgctga ttatcattat atgtaagcga acctttgcca
    21541 gacaaagtaa actttatcaa gtaagaaggt aaatgtgctc gttcagtata gtaagggatt
    21601 ggtgctttga agtaaccgat ttcttggata taaaaaaata aattcctgct tctttgtgta
    21661 ggcgtcaaga tcactctttt tgagtcttcc gaccatgagt ggttggcttt ttcccagtaa
    21721 tccatgtaaa cactcccaac ttgtcaagat aatgcaatta tataccaaaa tagtgtgaag
    21781 caaaatataa gattctgtat tatgatagca ttgtcaataa atgataacgc tttattaata
    21841 aagtgctaat caacaataaa gtaaaagagg tggagaaatg aatccaacat ttgttgccat
    21901 cgatatcggt gcatcaagcg gacgactaat gaaaagtacg agggatgcaa aaggttcttt
    21961 gatcttggaa gaagtacacc ggttcaaaaa cggctttcgg aacaaagacg gtcacgatcg
    22021 ctggaagatc gattatctta tcaaagagat tttgattgga tttgaaaaat taaagcagac
    22081 agggattgat cgcctttttg cggggatcga tacatgggca gtcgattact gtctgattga
    22141 tgaacaaggg gatcggataa atgatccgat tgcttatcgg gacaagcgta ccaatggagc
    22201 agtagaacag ttttctgaaa tctattctct agataagttg tatgagcaga cagggattca
    22261 gatccagcct ttcaatacct tgttccaatt gtttgctgaa gaaaaagaaa atttggcaaa
    22321 agcacataaa ttgttactga tacctgatta tttagggtat gtctttactg gtaacatggt
    22381 gacggagaaa actaatgcat ctactatgca gctgctgaat gcagggacaa aagaatggga
    22441 aatagaactt cttgaaaaaa tcggtgtctc gaaggaatta tttccaccat tgacagagcc
    22501 gggaacgata ctaggagatc tgcaaaaaga acaattttca aaatatgatt tgccagatgt
    22561 gacctttatc acagtagcaa gtcatgatac ggcgtcagct gtattaggaa cgccaggtca
    22621 aggagaacgt tggggttata tcagcagtgg gacatggtcg cttttaggga ttgaaacaaa
    22681 gatcgccaat gtttctcgca atgctttttc tgaaaattat acgaatgaat ggggtgccca
    22741 taacacggtg cgttttttga aaaacatcat ggggatgtgg ttgatccagg aagttgcccg
    22801 aatgcaagag tacgagtaca gttatgcgga attggcagag cttgctgcaa aagaaaaacc
    22861 gctgcagcaa gtgatggaaa tcaatgaccc tcgatttttg aatcccccaa atatgataga
    22921 agagattcag caatactgtt ttgaaacaga gcagccagtc cctgtctcat cgggagctat
    22981 cgctcgttgt gtgtatgata atcttgctct ttgttacgca caagagctga tcaaagtaga
    23041 acacttaact ggaacgaaat tggaaaagct gcacatcgta gggggcggat cgaataatcg
    23101 tctactgaat caattgacag cagattatgc cggaatcatt gtagaagcag gacctagtga
    23161 agccacagca atcgggaatg ttgccatgca aatgctcagt acaggtcagt tttcgaatat
    23221 agaacaagca agaaatgtaa tcaaacagtc gtttccatgt gaaacttttt acccagatga
    23281 aaataagcaa aacacgatgg aatggtacgt gaataaaatc aaaggagtaa tcgtatgaac
    23341 acgattgaaa gaaattacga acaagcaaaa gaaaaatatg caactatcgg tgtagataca
    23401 gatgctgtac tagaaaagat acaggacatc aaaatttcta tgcattgctg gcaaggagat
    23461 gatgtcaaag gtttcttgac accggatggc gaattgaccg gtggaatcat ggcgacaggg
    23521 aattttccag gagccgcacg aacacctgaa gagcttagac aagatctgga gaaggcttat
    23581 tcgttgatac cgggcaaaca caaattgaac cttcatgcta tctatttgga tacagaagaa
    23641 tctgtcgatc taaatgaaat tgagccgaaa cattttgaaa aatgggtaga gtgggcaaaa
    23701 aaagaagaaa tcggtcttga cttcaatccg actttctttt cgcatccaat gatgaaagac
    23761 ggctttacat tggctcatcc agataaagaa gttcgtgaat actggatcga acacgggaaa
    23821 agaagccgca aaatcgcgga gtactttggg aaagaaactg ggcaaacaag tatcaataac
    23881 ttttgggtac ctgatgggat gaaagataat ccgattgatc gttttacccc acgggaacgt
    23941 ttaatggaat cactcgatga aatttttaca gaagagttag atgaacggta tgtacaagat
    24001 gcagtagaaa gcaaactatt tggtttaggc gctgaagctt atacagtcgg ttcccatgaa
    24061 ttttatatgg gttacgggtt gactcgtgac aaacttgttt gtctagatgc tggacatttt
    24121 catccaacag aagtgatttc caataaatta tcttcattag ctttattcag caaaggaatc
    24181 atgttgcatg tttcccgtcc agtgcgttgg gattcagacc atgtggtttt gatggatgat
    24241 gaattgcagg aaatcgctaa agaattagtc cgtaacgaat tgttggaaaa aacaaatatc
    24301 ggtctggatt tctttgatgc aacgatcaac cgtatcgctg cttgggtcat cggtacgaga
    24361 aatacacaaa aagcactgct gaaagctatg ctggaaccag ttgagcgatt gaaagaaatg
    24421 gagctggctt ttgactttac ttcaagaatg gcttatacgg aagaattgaa agatttcccg
    24481 tatgccgatg tatggaatta tttctgttac aaaaatcaag ttccagttgg tttggattgg
    24541 ctggaagaag tccaggaata tcaaaaagac gtgttggaat taagaaaata ggagtgtggc
    24601 tatattgaaa aaaatagata ttttacaagc gccttatgtg aaagaaatga tcgaaacgac
    24661 gtcgaatctt tatcgcttgg gatgggatga aagaaatggc gggaatattt cttatttact
    24721 aaaagaagaa gagatcacac cgttcttgga tcctgaaaaa gtgatccgcc gcatctcgat
    24781 gatttttgat gccagccgtt tagcaggatg ctattttatt gtaactggtt caggaagata
    24841 ttttaaaaat gtagcgagcg aacctgccga gaatcttgga atcgttcgag ttgctgaaga
    24901 tggcaaaaca ctggaacttt tgtgggggct ggaaaacgaa tgccttccta caagcgagtt
    24961 gccaagtcat ttcatgagtc atatcgcccg tttagaagta gacccagata atcggatcgt
    25021 tatgcattgc catgctagtc atctattagc gatgagtttt acgcatgaat tagacgaacg
    25081 ttcctttagt cgtacgctat ggcaaatgtg tacggaatgc ttagttgttt ttccagaagg
    25141 aatttccatc attccgtgga tggttccggg aacaaatgag attggggaag caacagctga
    25201 aaaaatgaaa gaaactcgat tggttttatg gccacaacat ggaatctacg ggtcaggaaa
    25261 agacatggat gaagtctttg gtttgatcga gacagcagaa aaggcagcag aagtctatac
    25321 gtacgtcaaa gcgcaaggac caatcttgca aacaatcacg gacgaaaacc tgtggcattt
    25381 agcagatgcg tttggtgtca ctccaaaagc aggttatttg gaggaagttc atacgaaagc
    25441 aggtgtctga catggtgaaa aaagcttttt gcatgaaagt ttacccagaa aaacaagaag
    25501 aatacaaaaa aagacatgat gaactttggc cagaaatgcg tacagcactt cgtgaacacg
    25561 gggtcatcga ctattcgatc tatcttgatc cgagtacaag tttgttattt ggttacttag
    25621 tgatcgaaga tgaaaagtta tgggaaaaaa tggcaaatac atctatcaat caaaaatggt
    25681 ggaagtacat ggaagatatc atggagacaa atccagatgc ctctccagtc agttgggaat
    25741 tgatgccagt ctttgaactg tagcaacaag caactgggca accggttgct tggggttctt
    25801 tcgtttttat tgtgaaaaat aatacaaatt aggaggaata gaaaatgagt aatcgaatga
    25861 ttttaaatga aacatcgtat tttggaaaag gagcgattga gtcgatcgtc cctgaatttc
    25921 agcagcgagg attcggcaaa gcagctgtca ttactgacaa aggattgatc gagcacggta
    25981 tagcaacaaa agtaacagaa cttctagata aagcagcaat cgcttatgct ttatacgacg
    26041 ggatcgtacc taatccaacg atccaaaatg taaaagatgg agtggctttt gttaaagagg
    26101 cagaggcaga ctgtttgatc gcaatcggtg gaggttcgcc aatcgatacg gcaaaagcca
    26161 ttgggattat tttgacgaat ccagagtttt cagacgtcat tagtttagaa ggtgttgctg
    26221 atacgaaaaa tccatgttta cctatattag ctataccaac gacttctgga acagcagcag
    26281 aagtaacgat caactacgtc atcacagatg aagtaaatca ccgaaaattt gtttgtgttg
    26341 atcctcatga tatcccaatc gtcgcgttca tcgacagcga tatgatgatg ggaatgccga
    26401 aaaaattggc tgcatcaaca ggtatggatg ctatgacgca tgctattgaa gggtttatca
    26461 caaaaggcgc ttgggaaatg actgatatgc tgcatttaaa agcaatcgaa atcatcggcc
    26521 attctttgga agcttctgta gatggcgatc aaaatgggcg agaaaaaatg gcattaggac
    26581 agtatattgc gggaatgggc ttctcaaatg tcggtttagg actggtacac gggatggctc
    26641 acccgttaag tgcatggtac aacattcctc atggcgtagc gtgtgcagca cttttgccaa
    26701 ctgtcatgaa atataacaaa gaatatactg gagaaaagta ccgtgagatc gctcttgttc
    26761 tagggattaa aggagcagct gaaatgtctt tagaagatgt aagagaagcg gcttgtggtg
    26821 agatcgatcg gttgagcaaa gcggttggaa tcccagaaac catttctgaa ttaggtgtaa
    26881 aagaagcaga tattccagca attgcagaag atgctttgcg agatgtatgc acaccaggaa
    26941 atccaagaga aacgacagta gaagaaatca ttgctttata tcaaagtttg atgtaaaaac
    27001 gaagcggatc ttcaacatta aattgcctgt aaagagaaat ggtagtttat atctggatat
    27061 ctcttttaaa ttgatggtat ttccgctata atttttctta taaagaaata ggagaatatt
    27121 atatggaaaa taaaaagcct tatggaactg tattgatcaa agcctctaaa atactagact
    27181 atttagcgga aaaccaagat gtctctttgc agacgatcgc aaagggtgtg ggaatgacct
    27241 cttctacagt cttgaaaata ctagatactt tgttgatgat cggctacgta aataaaaatt
    27301 cagagaaaaa ctatcgttta ggtgcaaagc ttattcgcta cgcgaataaa aacattgaac
    27361 aaatcgattt ggtagagttg actttaccgt ttttagaaca gctgcagcaa aagatcgatg
    27421 agactatcca cttaggggta ttggacaaca atgagatctt gtacgtgaac aaactagatc
    27481 caaaacatca aacgatccgc atgtcttcaa aaatcggaat cacccgacca ttatacaatt
    27541 ctgcgatggg caaagccgta ttagcagaat tcagtgaaga gcagtacact gattatcttg
    27601 aaaagcatcc gttgatccct tacacagaat atacgatcac caatgcatta cggttgaaaa
    27661 aggaaatcaa aacggtaaaa gaaacccaca tcgcatttga cgatgaagaa atcgaacgtg
    27721 atattttttg tataggagcc tcaatcgtga aaaacgaaca aatcattggt gcatttagtg
    27781 taagtatgcc gaaatatcgt ttaacagatg agacaaaaga acaaataatc agtgccctca
    27841 agcaaacaaa aagcgaaatc gagcaaagaa ttcaaaaata atgaaaaaag cgtttgcaat
    27901 ttttcttttc tgccgtataa tatgggtata gatttccaag ttataaaata aaatttctaa
    27961 ataggaaata ggtggagaag atgaaaaaaa ttgatatttt atctcgattg aaaaacgccg
    28021 gagtgattgc ggtggtacgc ggaaaaagca aagaagaagc tttgaatgct tgccacgcaa
    28081 tcatcaaagg tggtctaact ggtattgagt tgacttttac agtccctcaa gcagatcaag
    28141 taatcaaaga acttttgtca ttctacaaag accaaccaga aatagtgatt ggcgcaggca
    28201 cagtattaga cgctgtcaca gctcgcttgg ctatcttagc tggcgcagag tatattgtta
    28261 gcccttcctt tgatcaagaa acggcagaga tgtgcaatct gtatcaaatt ccttacttac
    28321 caggatgtat gactattacc gagatcaaaa cagcgctgaa aagcggggta gatatcgtca
    28381 aactatttcc tggaagcgct tatggaccaa gtatcatttc tgccttcaag gcgccgatgc
    28441 ctcaagtcaa tatcatgcca actggcggtg tgagtctgga caatatgaaa gagtggttcg
    28501 atgctggtgt cgtaacagtc ggagtaggtg ggaacctttt agcacccgca gctacaggag
    28561 attttgacaa agtaacggaa gtcgcgcaac agtatgcagc aaaaatgaaa gaaatcaaga
    28621 ggtagctgct tatgggaaaa gtagtgacac tcggagaaat catgttgcga ttgtccacag
    28681 agcagggaac ccgtatcgat tcaagtgaac gattcgctgc ccattatggc ggaggggaag
    28741 caaacgtagc aatttcttta gctaattacg gacatgaagc gatttttgct acaaaagtac
    28801 ctgataacgc attgggaaaa gcagtcaaaa agcacttgca atgttatgga gtagatacga
    28861 aacaggtatt atctggtgga tcaagactag gtacttatta tatggaaact ggtagtggcg
    28921 aacgcgccgc ctcagtgatc tatgatcgtg ccggatcaag ttttgctgaa atggatcatt
    28981 tggaatggtc gctagaggag ttattccaag gaatagatat atttcatgtt tcaggaatca
    29041 ccccagcttt gtctaaaaaa tggcaaaaaa tgacgaaaca ggtgatagaa gctgcaaaaa
    29101 aagctggctg cctgatcagc ttcgatatca actatcgtgg aaaattatgg acacagcaag
    29161 aagccagcga agtaatccat cagcttttac ctttagtaga tatctgctca gctggaaaaa
    29221 tggatgctct ttatttgtta ggtatcgaga aagcgccaga agcagaagaa caacctctaa
    29281 tttattatta tcaaaaaatg caggaaaagt tcccgaatat tcaggtgttt tattcgacca
    29341 agcgtaccgt tcattcagca agtgaaaatg aattgaaggg gacattgtgg atgaaccatg
    29401 aatatgtgga atcccaagtc catagaatca ccccaatcgt ggatcgagta ggtggcggag
    29461 atgcttttgc aggtggcgtg ctccatggga ttttatccaa tatgtctccg caagaaatca
    29521 ttgattttgc tacggcagct tctgccttga agcatacgat ccatggggac tgcaaccaat
    29581 ttagtcaaga agaagtcgca caatttttag catgcggctc tggaaaaatc attcgataga
    29641 aggagtagaa gaaaatgcaa gaaatggaaa cacgttatac acacagtcca gaagatatcc
    29701 gtcattattc aacggaacaa ttgagaaaag aatttttagt agaaaaggta tttgaaccag
    29761 ggaaaatcag tttgacttat acgcacaacg atcgaatgat ctttggtgga gtgacaccta
    29821 ctgaaaaaga attagaaatc acattagaca aagaactagg tgttgattac ttcttagagc
    29881 gtcgtgaact aggtgtgatc aatatcggtg gaccaggatt catcgagatc gatgggcaaa
    29941 aagaagaaat gaaaaaacaa gacggatact atatcggaaa agaaacacga catgttgtat
    30001 tttcttctca agatgctgaa aacccagcta aattctatat tagttctgtt ccagctcatc
    30061 ataaatatcc aaatgtaaaa atcagtatcg accaagtaaa accaatggag actggtgaag
    30121 gcctgacact gaatgaacgt aaaatctacc aatacatcca tccaaatgta tgcgaaagct
    30181 gtcaattaca aatgggttat acgattttag aaccaggaag cgcttggaat acgatgcctt
    30241 gtcatacaca tgaacgtcgt atggaagctt atgtctactt tgattatgcc aatgaagata
    30301 cacgtgtgtt ccacatgatg ggcaaaccag atgaaacaaa acatttagtt gtagacaatg
    30361 aacaagccat tatttcgcca agctggtcga tccattcagg tgtaggtaca agcaattact
    30421 cattcatctg ggcaatgtgc ggagaaaata tcacctatac agacatggat atggtgcaaa
    30481 tggatcaatt aaaataagca taagggagag aaaatgatgg aatttagtat ggatatgttt
    30541 cgtttagatg gaaaagtagc attagtcacc ggtgctgtct atggtattgg atttgagatt
    30601 gcacgctctt tagctacagc aggagcaaaa atcgtattta ataacttgaa tcaagaatca
    30661 gttgatgaag gtatagcgca ttataaagaa gcaggcatcg atgcaaaagg ttatgtctgc
    30721 gatgtgaccg atgaagaagc ggttcaagcg atggttcaac agatcaaaga agatgtaggt
    30781 tcagttgata ttctagtcaa caatgcaggg atcatcaaac ggacaccgat gatcgaaatg
    30841 gatgcggcag acttccgcca agtaatcgac gtggatttaa atgccccatt catcgtttca
    30901 aaagcagtta ttccggatat gatcgaaaaa ggcggcggta aaatcatcaa tatctgctcc
    30961 atgatgagtg aattaggacg cgaaacggtc agtgcttatg ctgcggcaaa aggcggatta
    31021 aaaatgctga cgaaaaacat cgcttctgaa tacggtcaat acaatatcca atgtaatgga
    31081 attggaccag gttatattgc tacaccgcaa actgctccat tacgcgaaac acaagaaaac
    31141 ggtgaacgtc atccattcga tcaatttatc gttggacgta cgccagcagc acgctggggc
    31201 gagccaatcg atttggctgg accatctgtc ttcttggctt ctagcgcatc tgatttcgtt
    31261 aacggccatg tcttgtatgt ggacggaggc atacttgcct atatcggaaa acaaccttaa
    31321 aagagcttta gtacactgtt tagtggagag gttgtgaaat cagcggattg aacgaatacc
    31381 gaaggacagc tgattaaaca tcatttattc gaaaaaagag gctgggacag aagcatttaa
    31441 ctccaagaaa taagaaggaa ttcacgaaaa ttgcttttca aatttttgtg aatttcagct
    31501 tatttccgaa ggagttgctt ttacttccgc cgtttgtacg tgtttagagc gtgagacaaa
    31561 agtgttcttt acttttgtcc cacgctcttt tctgtgcgtc ttccatactt cactaagtcg
    31621 atccctcttg cttcgcacca atccttcact ggtccggaaa agatcagaga ttgttggtgt
    31681 agggccgcgg tattcgttgc gccgaccata gtataaagca gacgaagctc ttcttgccat
    31741 tgcttcatca gtataatagt ttcttctacg ccgtgattca tcagatgcgt cagtacggtt
    31801 cctgaagtac ccacggcatt tgcgccgaga caaagggctt taaatatgtc ataggcattg
    31861 cggatacctc cggaggctag gatttccata gaagtgtctg cttcatttgc ttcaagcaat
    31921 gaagagactg tcgattgccc ccagtcagct aaataactca gttcccgctt ggagcgacgg
    31981 gcgttttcga tctgggtgaa gcttgttccg cttcgaccgc tgatatcaac tgtatgaact
    32041 cctaaagcag ctagatcatt gagcgtttct cttgtcatgc caaaaccgac ttcttttacg
    32101 atgaggggga cgtcaatggc tgtttgagtt tcttgaataa gtactttcca attggtaaaa
    32161 tcacggtctc cctcaggcat aaccaactct tgaggtgcat tcaaatgaat ctgtaaggca
    32221 tctgcgtgaa acaatcggat agcttctttg gctctttcga cagaagtccc tgcaccgatg
    32281 ttggcaatga ttttcccgtg aggatattct tgtcgcataa tcgtataagt atcggctaaa
    32341 gaagggactt tcaaggcagc gctgactgaa ccagtggcaa tcatcaaatc tgcttctctt
    32401 gcgacgattg caagatcttg gttgattttt tttgtttttt cgcttccgcc agtcatcgca
    32461 ttgatataaa atgggctgct tagtgtaaac ccaactgcct gtgtacttag atcgacatca
    32521 gaaactgcta tttgcggcaa agggttatgg atgatccgta cgaagtcaaa ttcgtttttt
    32581 tgtttatcat gaaaggcttt agctagtgaa acatgttcgt cttttcgatt catcggtttt
    32641 cctttctttg atcgctatag acatgtaacg gcagtggagt gatttctgct ttttcccatg
    32701 cactcattaa aggaagaatg ccagatttct ggtcaacaat tacgattccg caatcgcccc
    32761 cacctgcacc agaagatttt gcggctcctt catactgttc agctaaatta cacaatttgt
    32821 tcaaagcagg cgtttcgatg acgaccccag tgattgcaga taaatcatgc agtaattgtc
    32881 ggttttttcg aatcatctgt tggatcaacg ttacattatt ttctttaaac cctttgatca
    32941 tttcgttgac acattctgta ctatttttta agaaaagctg ataagccacc attttatctt
    33001 ctctcgaacg gtgaacttga tcgaccaaat cagaagtaga ggcagggcta cccgtccaac
    33061 caatcaataa acgtaaatct tcaggagcaa tcaatggctc aatggataga cctggccaat
    33121 ccagggcaag taactcactg atagaatgct gagtttcttg ttcttggagc caaggatggt
    33181 cgaaggttga gaaagcgatc cagccaccat aacagctagc agcgatgtcg ccgcaggaac
    33241 cattatcttg aactgctaaa ttggctagtg ctgcaatctt gaaaatctcc aactgagata
    33301 aattcaacgc gtaaaaaaca ttcaaggctt tgaccgttgc aacggtcacg gcaccgctag
    33361 aacccaatcc atatttccgg ccatttgaac tgtctaactc acttgttact ttcagatcat
    33421 aaaaggataa aagaatgttt ttttcttgtg catacttttc agtcaagcga atagcagcaa
    33481 ggatataatg aaaaggattt tcccgaatat ctaaaactaa ttctccgttg cgtcttgtcc
    33541 aacgtacagg catcccacta tattgtgcag attggatact tccgactttt cgtgcagatt
    33601 ctacagtgac tgtcacgaat tgatcgactg cagcgataac tgctggatgg cctgtttcaa
    33661 caactgcata ttctccggca atatagagtt tgcctggtgc agatacttca atcataaaaa
    33721 tctcccttta ttcaataatc gcaattcctg gaccagcatg tgcagtaatt acttgttctt
    33781 tgctaaataa tttggtaaat gtttcttgta cctcagataa atggctgttt tccactaaaa
    33841 ctttgacatt agggccagca tccatcgtaa agtagcatgg aatgccttgc tttctcaatt
    33901 gtcgcaccgc atccatcgct tttaagctgt caggagacca gtaagtgaat ggaggctggg
    33961 cagctaaagt cgttccatgc atttttagtc cgtttctttc catcgtttct ccgagaagtt
    34021 ggaaatcttt tgttttgatt gcttgtttca attgatatag atccccttca acggaatcta
    34081 accagccttg ataaaaactg gatgtttcga ctgtccgacg catcccattt ctgctggaca
    34141 cttctttttt ctggtcgttg atcaaaacga aaaccattgc taaatcgtct tcgaaagaat
    34201 cggaagggac tggcttagcg taagaactta agtcgtcatg gcctttttcc cattcgacga
    34261 aaccaccgaa aatacttcgg caagctgaac cagacccgcg tcgagcaaat cttgaaagag
    34321 agagatcatc taatccaagc ttaagagcag tattgcaagc gcctgctaaa gcagctagcc
    34381 cgctggcaga ggaggctaat ccagctgcgg taggaacgaa attctgactg attactgaag
    34441 cttttagtga gaggccggct tctttacgaa aaaggtcaag aaactgactg actttttttg
    34501 ttgctttttc gtcttgcaag gtgccatcta gataaaattc atctaccata tagctatcag
    34561 aaaagatgac ttctgtctct gtataaaatg catccaatgt taacgaaagg ctgttgttca
    34621 ttggaaggat gagttcttca tttttcttac cccaatattt gattagagca atgttggtat
    34681 atgcgcgtgc tttgccttta aacataggta tgtactccta atccttgtat ccaagtttca
    34741 gcagcacccg catcttcaag tgcttgactg atttcttggg cttttgtttt tgtttgtgct
    34801 aatgcaatca tacagccacc gcgtccaccg ccagttaatt tggcacccaa tgccccggta
    34861 tcttttgctg tttggatcaa tagattaaga aaatcattgc tgattgtcag cttttccaga
    34921 agtgattgtg attcatccat tgtctgtgcc aacgtttctg gtgaattttc aatgattgct
    34981 tgttttgctt gcttcgtcaa gtatcctaat ttttggatca tttgtccagt ttcatgaggc
    35041 tgggtttcaa agaggtgagc gacatctttg acggcttctc ttgtttgtcc tttgattcct
    35101 gtatcagcga caatcaaaaa agcatcgatg ttcaaagaaa agtagtcgaa aggatgccct
    35161 tttgtaaaat aaatcggctg caagctgcta gtagcggctg catcgattcc actaggatta
    35221 ccatgggcga ttttttccga aagctggaca ttttctaata gtatttcacg agacaaagga
    35281 aatgctaagt aatcataaaa agcacgagtg actgctgtgg cgactgcagc gcttgatccc
    35341 attccacgtt cagcaggaat cgtgctttca atggtcagtt gcaagttttt ttggatgtca
    35401 tgctgttttt ttaagtgaat gaataatttt ttgatatttt ttaatgcatg aggagcttcg
    35461 gctaaatttc ctgaatagta ggaagaaacc agttgatcgt ccattgcatc cagctcttca
    35521 aggaatgcgg tgacttttgt tgcatagaaa ggaaaggcaa tcgctggttc tccataaaca
    35581 accgcgtgct cgcccatcaa tatgatcttt ccgcttgatt ctccttggcc atagtttgcc
    35641 ataatctaac tccttctcac gtaaaagtga tttttaagca aaaaataaag cgcccaaaac
    35701 gagttgcgct gcttcacgaa tttttttgtc cactaagggt ctcaaaaaac attcctcctt
    35761 tattttacta aaaccatctc agaagtaaaa ctaagaatac gttttctaaa gaaaaaacac
    35821 ttttttctta agaatttttt gacttttttt cttctttgac tagaaaaaaa tacaattgac
    35881 actactagat attgggggta tatttaaaaa ggttggagtt aaacacaata tttagtgatg
    35941 aagtgagctg gaggtaaatg agcgtgatcg ttttagcagg tacaattggt gccggaaaat
    36001 caagtttaac agaaatgatg gctgaacact ttgatagtca ggcattttat gaatcgattg
    36061 atgataatga agtcttgccc ttattttatg caaaccctga acagtatgct tttttgctgc
    36121 aaatttattt tttgaataaa cgttttgcaa gcatcaaaca agcaatgaaa gatgacaata
    36181 atgtattgga ccgttcgatt tatgaagatt cactgctttt ccatctaaat gcagatctag
    36241 gaagagcaac agaaacagaa gttcgtgtgt atgatgaact gctggaaaac atgatggaag
    36301 aacttcccta tgctgcccat aaaaaacatc cggatttatt agttcatatt cgtgtatctt
    36361 ttgatacgat gttggagcgt atcgaaaaac gtggtcgttc gtatgaacaa ttgtcctttg
    36421 atccaagtct atacgattat tacaaagaat tgaacagacg ttacgatcaa tggtatgaag
    36481 aatacaaaga aagtccaaaa atccaaatcg atggagaccg ctataatttt gtggaagatc
    36541 ctcaagcaaa agaagaagtc ttgaaaatga tcgaagaaaa attagcagag attcgccaaa
    36601 ccaaagtagc catttgattt acagttgctc aaataaaggg taaaccttta tttgagcgac
    36661 ttttttcttt atactggaaa caaatgtcag gaggatagag tatgcgggaa aaacactatt
    36721 ttaatcaaga ccgtaatttt tggatcaatg tacagcaaaa cgatgtgcta gctgttaaaa
    36781 acttaaaaga agaatatggc attagtgacg agatgctgac ttattctcta gataaaaatg
    36841 aacgggcgcg cgtagagtac gattcttttg atgaagcgtt actgcttgta tcaaatgttc
    36901 cgcaccagca aaaagtcgat aatcattatg aaactagtcc tatcgctttt attttaaaag
    36961 aagacggttt gtttacattt accacgccga ataccgagta cgtgattcga ctaattcgat
    37021 cgcttttgga acgaatgcca gacatgagtg tatacagttt attgtttcgt acattgtttt
    37081 tgatttctga ttcctttttt cctttaatcg aggaagtgaa tagtgaacgt cagcggctta
    37141 atctgaaatt aagagaaaaa acaacgaaca agaatctgct gcaactttca gatttagaga
    37201 tcggtttggt ttacttagtt acaggaacaa aacaaaatgt agtactttta gaacaaatca
    37261 aggcattggc tatttatcgc aagttgtcag aaaaggaaaa agaagagttg gacgatgcgc
    37321 tgatcgaagc acgacaagca gttgaaatga cgaacttagc ttcgcagatt ttagaccagc
    37381 tctctggcac ttataataat ttgctgaata acaacttgaa tgacacgatg aaatttttga
    37441 cggtctggtc gctgatcttg accgttccaa ctattgtgac aggattcttc ggaatgaatc
    37501 tccagttacc ctttacccat tccgttttcg gatggggaat cgccttgatc atcagtttgg
    37561 tcttatctat ttggatgctt attgcactgt ggcggcggat tcgttgatct agtctttgat
    37621 ctgcaaacca agggcttggg cgatcacaag tgcttgctct tggttgacga ttagtcccgc
    37681 caaagaatct tgagataaag cgattgctgt gaagaaatta gtcgtaaagt ctaatccctt
    37741 cagagaagtt cgaaaccagt tcgaaccggt caactgattg tcagacaaaa aaaggttttt
    37801 ccaagtgacc tcataaaatt cgctgtcttc taattgacaa tgatcgaagg agacagcttt
    37861 caagtttgta ctactaaagg aagccatcga agcaatacaa tcagtgaaag tacagtctcg
    37921 aagataactt tcggcaaaat tcgtacctgt taatttacat tgatgaaaca cgacttggtg
    37981 gaaactgcca ccaatccact ccagattgga aaaatcacat ttttcaaaaa tgacattact
    38041 gcattcaaaa tgacgtaaaa ctgttttttg catcgtaagt ttttggatat gactctctct
    38101 taataccata tttttggcat cgagatagct ttggtcaccc ccgttgatca gaagaccaga
    38161 gaagatctcc tcatcttcta aataaaactc tccctttgta agaagaggaa gagaaggtgg
    38221 aataggttcg atttttttca tgtgggttca cctcgttttt ctttagtata gcaaagaagc
    38281 gggaatcact gctagaaagt ctgctaatct aatcattttc agaaaaggaa gacaaactgt
    38341 atgaaaaaca ttgacaaaaa gattagtttt tgctatacta tgatggttga gaaataaaag
    38401 cagaagcacc cgcttctcgc cttagttgat aatgtcactg ggctagatag ttttcactgg
    38461 ttctattagg tgaagtacgc gcgggttctg taagagaact cgttttttta ttgccgttta
    38521 ttcggtaagc ttttctttct ctcataatat ttggaggtga atgaccatag caaaggatat
    38581 gatggttaac gacggcattc gtgcacgaga gttacgattg atcggttcag atggtgagca
    38641 attaggagtt aagacaaaag cagaagcatt gaatattgca gaacaagcaa acttggatct
    38701 tgtgttagtt gcccctggtg cgaaaccacc agttgcgcga atcatggact acggtaaata
    38761 ccgtttcgaa caacaaaaga aagaccgcga agcgcgtaaa aaacaaaaag tgatcaatgt
    38821 aaaagaagtt cgcttaagtc ctacgatcga cttgaatgac ttcaatacaa aacttcgtaa
    38881 tgcacgtaag ttcttggaaa agggcgataa agtgaaagct tctatccgtt tcaaaggccg
    38941 tgccattacc cataaagaaa ttggtcaaaa agtcttgaat cgcttagctg aagaaactgc
    39001 ggatattgca acagtggaac aaaaagcgaa aatggacgga cgcagcatgt tcttgacgct
    39061 ggcaccgaaa aacgacagta agtaatttga atgactaact ggctgttgga cagccgatag
    39121 tcggataacg aaagattttg aggaggaaat atagtcatgc caaaacaaaa aacacaccgc
    39181 ggatcagcaa aacgtttcaa acgtactggt aaaggcggat tgaaacgctt ccgcgcgttt
    39241 acaagtcacc gtttccacgg caaaacaaaa aaacaacgcc gtcaattgcg taaagcaagc
    39301 atggtttcta gtggcgactt caaacgtatt cgccaacaac tagcaaaaat gaaataagaa
    39361 gtacaaataa cagaatctga agatttattt ttattaggag gaattataca tggcacgtgt
    39421 taaaggtgga acagtaactc gtaaacgtcg taaaagagtg ctaaaattag caaaaggtta
    39481 ctacggttca aaacacactt tatttaaaac agcaaaagaa caagtaatga attcatacaa
    39541 ctacgcatat cgcgatcgtc gtcaaaagaa acgcgacttc cgtaaattgt ggattgctcg
    39601 tatcaacgca gcagctcgta tgaatggctt gagctactca aaattaatgc acggtttgaa
    39661 agttgctgaa atcgacatca accgtaaaat gttagctgac ttggctgtta acgatgcagc
    39721 agcatttact gcattggctg accaagcaaa agaagctttg gctaaataag ctaaacaatt
    39781 ataaagagga aagaagataa cgaaaagtta tgttctttcc tcttttttta tttacaatcc
    39841 tccatcaaat gaaacaggac aataacgtat aagtctaaaa tagcataatt aaggtttttt
    39901 aattgaaaac tgaacattta tggtcaccct ttaatgaaag cgctaactac ataagttaga
    39961 atgagtttga ctgatatatt agtttgctta tcagaaaaac aaaatggacg gaggaataac
    40021 atgagtgata ttattattgg tattttgtta gttatctcat tctttttcat ggtctggtac
    40081 tgtgtaaaag gctacaactt gatggtcggc tttgccatca tggctactgt atggatggga
    40141 ttagcgctag tcggaaacac gttttcacca aacccggcga tggaagggca aggagtaatt
    40201 gatgtgttga cgcatatcta tacgacgggg ccagctgagt acgccaaatc gatcttggta
    40261 aatgtcttct ttggtgcctt tttcgggcgt gttctagtag atagcggaat cgcagctacc
    40321 ctgattcgaa aggtagttga acttggaggg gacaagccaa gaatcacgat gagcttacta
    40381 tgtattgtaa ccgctgttat attcatgtcg atgacaggaa tcggaccagt gatttctatt
    40441 gcagttattg tgttgccaat cttgatgtca ttaggtattt cagtaccggt tgcactgttc
    40501 tcatttatgg gctcgattat ggctggaatc tttgctaata tcgtcaattt caaacaatat
    40561 caaacgattt atgctggttt caatccagcg gctgaaagct atacttataa tgactatttc
    40621 caaattggta tgattgggat gattgtaagc ttagttgtgg tactaacggt cgctaatata
    40681 tcaatgaaca aaaagaaacg ctatgcaatg gcagcaaacg taccagctga aggtggcgac
    40741 gcaccaatga tctcatggtt agccgtatta ttaccagttt taggggtcgt tcttttagat
    40801 cttccaatca tcttaggttt tattctagca ggtatctggg cattgctgtt tactggaaaa
    40861 ttgcgcggag gttataaaga aatctgtcgt cagtttgcga aattatttac agatggcgca
    40921 gtagatgttg cgccaatggt tggtttcttg atgaccttag cgatgttcaa taattcagct
    40981 gcgtatgctt ctccttattt ttcagctatt tttggagatt ggataccgca atcaccatta
    41041 gtcttggcaa tcgtatttgc tatattgaca ccactaggat ttttccgcgg tccaatgaat
    41101 ctagtcggtt caggctcagc tattttagct gtcgtattag ctgttaatcc aacgatgtcg
    41161 ccagcctttt tgttcccgtt atttgcaatc acaacgattg ctcctcagca tttagatatt
    41221 acacaatctt gggttgcttg gggattaggt tatacaaaag taacttctcg agaatatatg
    41281 aagaaatcca tcccaactgg ttggatcatc ggagcgatct tatgcctgat cacgttctta
    41341 ttgtacggga acgcctagta aacaaaacgt cgataaaaac gattagatga atggaggaaa
    41401 agaaaatgaa acgttttgtg cgaatgggaa ttgatgttgg cggtacacat acgaaagcag
    41461 tcgcaatcga taatgcgacg cacgaaatca tcggaaaatc ttcagtgaaa acaacccacg
    41521 atgatgtccg tggagtagcc gcaggtgtcg ttcagtcttt tcaaaattgt ttgctggaaa
    41581 ataatatcag tccagaagat gttgtgtttg ttgcacattc taccacacaa gcgactaatg
    41641 cactgattga aggagacgta gcaaaagtcg gcgtaatcgg gatggcaaaa ggtggattag
    41701 aaggtttttt agcaaaacga caaactagat tgaacgatat cgatttaggg aataagaaaa
    41761 aaatagagat tgtcaatgca ttcttacctg tcaaacactt aaatgtagac cgtgtttcag
    41821 aaacgatttc ttctttagaa agagaaagag cggaggtctt ggtttcttct atggcatttg
    41881 gagtagataa tggggaacca gaacgagttg tttatgaagc agcatcggta aaatcgatcc
    41941 ctacaacgat ggcttctgac atcacaaaat tatatggatt gacaagaaga actcggacag
    42001 cagcaatcaa tgcatctatt ttaccaaaaa tgctagatac ggcaacctct acagaagatt
    42061 ccgtacgaga agcaggggtc aatgtttctt tgatgattat gcgcggagac ggcggagtaa
    42121 tggaaatcaa tgaaatgaaa aaacgacctg ttttaaccat gctttccggt ccagcagcat
    42181 cagtcatggg ctctttgatg tacttgagag cgtctaatgg tgtttatttt gaagtaggcg
    42241 gaacaacaac caatatcggg gtcattaaaa atggacgtcc agcaatcgat tattcaattg
    42301 taggtggcca cccaacatat atctcttcat tagatgttcg ggttctaggt gtggcaggcg
    42361 gttcgatggt acgggctaat cagtcaggaa tcatcgatgt cggtccacgt tctgcacata
    42421 ttgccggtct agattatgca gtatttacgg agacagaaaa aatcaaaggt cctaaggttg
    42481 aatttttctc tcctaaagaa ggagaccctg ctgattacgt caaggtagtg atggaagacg
    42541 gagaagaagt gactattaca aatacatgtg cagccaatgt ccttggatta gtgcaagaag
    42601 aacatttttc ttatggaaat gtcccatctg cacgaaaagc catccaagca ttagctgatt
    42661 attgtcatac gacggttgaa gacatcgcag aacagatcat ggaaaaatcc tatgctaaga
    42721 ttgaaccggt gattttagaa ttggcagaca agtatcactt ggagaaagat cagatttctc
    42781 ttgtaggtgt agggggcggc gctgcctcat tgattactta ctttagtaat aaaatgggtg
    42841 tgaaatattc gattcctgaa aacgccgaag tgatttcttc tattggtgta gcacttgcaa
    42901 tggtccgaga tgttgtagaa cgtatcatcc cttcgccaag taaagaagat atacgatcgt
    42961 tgaaaaatga agcgatgaat aaagcgattg aatcaggcgc tacaccagaa tcgatcgaag
    43021 tccacgtaga aatcgatcca cagacttcaa aagtaacggc tatcgctact ggttctacgg
    43081 aagtgaaagc aaccgatcta accaaggaga tcacaacaga agaagcacta gagcttgctg
    43141 cagaggatat gcgattaaat aaaaatgaag tttgcttatt ggaaaataca cctttctttt
    43201 atgtatgcgg cgaacagaat cgttcaaaaa atgctggaag tcttcggatc atcgatcaaa
    43261 aaggatttat caaggtccaa cgtggacatg cttcatgtat gaaaacaact gcagccaatt
    43321 atatgacagc tgtcgaacaa ctttgggaag atatggcagt ttatcaaact gaattgattg
    43381 cccgaccaga attttatctc tgcttaggag caagagtctc tgattttaca gcgacggatc
    43441 tggaacaact acagcttttg atggatctgg aagtatctac tatggaaccg gaggaagaag
    43501 tgatcgtcgt tgccggaaat atcaaacaaa cataagttag aagagttact aaacgaatta
    43561 caacagatcc ccgaagaaat ttggggattc taccagtttc ggcgagattt attttggaaa
    43621 aagattcctt tgtccaagca gaaaattctt atacaacaat caatcgattg tggtatcgaa
    43681 acggcatgta gcatcaaaaa aaagtaccct tttgccgatg taggagaaat ttgtgaacaa
    43741 atggctgttc caatcgtttc ctgtgaatca gagcaaataa acgaacgaat cacttttgca
    43801 acttatgcag aagatgaagg gattcgattg atgacggagc cgttagaaaa attaaaatgt
    43861 tcaggactag cttcgatctc taaagaaaca gcgcaagctt tgattattgg acatgaacta
    43921 tttcaccaca tcgaagcttc tgttaaggga atttatacgc aaagcgaaaa aattgtgctg
    43981 tggcggttgc ctttttatac acatcaatca aacattcgtg cattgagcga gatagcagcg
    44041 atgagttttt caaaagagat gaatcaaagc gatttttcgc cttatgtatt agaggcagtc
    44101 ttactttggc catataatga aattcgtagc caagggatct tagaagaaat aaaaaaaata
    44161 gaaaaaagat gtacggagta tgatttagat tacaaataaa gagactgtga caaaagtcgt
    44221 gtcacagtcc tttttttccg aataaacgat gttctcctgt caactcctcg gtcacaagtc
    44281 aaaatattca acaaacatga gaagctgttt tttgaaattg ttccttgtga ctcggagatg
    44341 aacggcaggc tcacgacctc ttggaaaacg gtgttcaaaa gcttgcatct gcggtaataa
    44401 gtccagccaa gttaaaaaca taagaagcta tttttacttg gctgttctta ttacttgatg
    44461 cagggcagct ttttcacaac ctctcaataa acggtgtgca atcagctgac cctcggtatt
    44521 agctcaaaaa aaacggcaaa atatgaggaa ctattttgcc gtttctttct ctaatactca
    44581 ggtcaaaacg ctgattccaa aacctcttat ttgatgggtc tgtcctgaat atcttcaaaa
    44641 taatcggggt aattatgaat cacgtattct tgctcctcga taatcatatg taaatgcatc
    44701 gtgcaaagaa atccgacttc atcaaagtta tgatcaatca ttgattgaag cattgtctga
    44761 tgttcatcta atactcgacc ccagtctaat tctgaaatcg tcaaacgtaa ccatctgaaa
    44821 cgcgtactat aactttctag ccaatcccaa atttctcgtt ttccagcaat ttcataacaa
    44881 gttcggtgaa aatcagtatc caaatcaaaa aaagcttttt tattttgagc gattactgcc
    44941 tgatcggttt gttctagtat tttttctagt ttagcttgat cttgcttggt cattttcgca
    45001 ctgcattctt gcaggatcgg tttttccaac ttttcccgcg tataacgggc taatgaagca
    45061 gatcggatat cgatttttga aacataagtg ccggactgtg gaatggtttt tacaagctct
    45121 tgattgcgca gttggatcag tgcttctcga atcggtgtac gcccgatagc tagttgttct
    45181 tctaacactt tatcggagat tttacttcca ggtgctaact cggaataaat gatttgttta
    45241 cgtatagatt cataagcttg ctgttgcaaa ttattgataa ccatttaacc ttcctttcta
    45301 tgcagatata atctattata acataaatcg cttacaaatc gtgtgggcat aaaaacacag
    45361 tctgcaaaaa aagccctgat aggcatgcct attagagcct cgaaaagaaa aatattgaat
    45421 caatctggtc gtacctcttg gatcggttta acacgaccgc cgattctttt agcaaaagct
    45481 tcggattctt ttttgtcttt gaaaatgagc agattgtcta agtttttttc ccgagtgcga
    45541 cagccagttt tatcgatgta accgtgcatt actttaacga catacatggg gatcacctgc
    45601 ttttttcttt tcattatacg aactactcga cacaatacaa gaaaaatgct tagacggtct
    45661 ttattaagaa tttacatcat ccaaagaagc gtacgcttgg aataactgat ctaactccgg
    45721 attgactttg ctcagcttca ctaatcgttg cgaatcagaa agtaaaaaac agtgtttact
    45781 actgccagta gtttttaatt ttttgtcttc agttcccaca atttcgtagc taaaatccaa
    45841 ccgtgtcccc gtatactttt ggatagttgg atggatgctc actttttctc cgtaatggat
    45901 catttcttta tagctgcaat ttacttccag aacggggatg atgatcccag attcttcgat
    45961 ttttttatag ggaaacccta gatgtaccaa taaggctacc cgagcctctt caaaccagcg
    46021 gatataattg ctgtgatgaa taatccccat ttgatctgtt tcataataaa aaggttctcg
    46081 cacataacct gtaaatttct ccatgactca tgcctcgttt tccattgatt ttttacaaaa
    46141 ataattcatc catttcggaa gtgcttcgct gatttgtgtc ggcaaaacaa tatattgttc
    46201 ttttgccagc tggtctccaa tgaaactatg aagataaata gctgctgcaa tcgtttggtc
    46261 attttgagga aactgggcta aaaatcctgt gatcatccca gctaaagtgt cacccatgcc
    46321 gcccgttgcc atagcagcag aacctaaagg attggtgtag aagtgctggt gaccataaat
    46381 ttttgtctca tgacttttca aaacgagcac agcaccgatt tcctgttgct tctttttgtt
    46441 ttctttatcc cgttgttctt gtatcggtag agaactcaac cgttgccact ccatttgatg
    46501 aggtgtaagt acagtttgtt cgggataggg tagcaaaaga tgattagcag caagtaaact
    46561 taaagcagag ccatcaatca ccagccattg ttcttttgat tggttttcta gtacttgttt
    46621 gaaacgttcc aaactcttag aagaagtacc caatccggga ccaatcaaga gtacatctgc
    46681 ttgctttatt acggaaagaa actggtcagt atccgtccaa actacaatca ttgcttctgg
    46741 cagatgactg tgaagagcac tccgattttt ttcatcagtt actacagtcg tgagacccgc
    46801 tccgctgtgt acggcagcta aagcactcat aatgattgct ccgccatatt gttcattgcc
    46861 gccaatcaaa acgatacgtc caaatgttcc tttatgtgaa atttttggac gaggtgtgat
    46921 gactcttttc actagttgtt cgtttatcag catctaaaaa cctcctttta ctcattatag
    46981 cgtatatttt ttttagagaa aaacagataa gaaaaggaat tgtcagattt tttgaactat
    47041 ttttttctgt aatttgtggt atcatgaaca cgaagactta aataagtgga ggattaactt
    47101 tatgacaatt gattggcaaa aagaagtaga agcacgtaaa gaagacttgc ttgaagactt
    47161 gaaaaatctt ttacgtgtca acagcgagcg cgatgactca aaagtaacac cagatgcacc
    47221 atttggacct ggacctcgcg acgcattgaa acatatgtta gcttatggcg aacgtgatgg
    47281 atttaaagta aaaaatgtcg ataactatgc agggcatatt gatcttggcg aaggagacga
    47341 aacgctaggg atatttggac acatggatgt cgttcctgca ggagatggct gggacacaga
    47401 tccttatgaa ccagtaatca aggatggaaa gatctttgca cgcggatcaa gcgatgacaa
    47461 aggtccaagt atggcagctt actatgcgat gaagatcatt aaagaacttg acttgaaact
    47521 atcaaaaaaa gttcgcttcg tagtcggcag cgatgaagaa agcggctggg gcgatatggc
    47581 ttattatttc gaacatgaag aagaaccaga ttttggtttc tcaccagatg ctgaattccc
    47641 aatcatcaat ggtgaaaaag gaaatgtttc tttagcttta cgcttcaaag gcgacaatgc
    47701 aggagattat gtcttgaaat cttttgtttc cggacttcgc gaaaatatgg tgccaggaac
    47761 agcaactgca gcacttcaag taccaagtgc agatgcagct atcgcgatgg aagaagcatt
    47821 ttaccaattc atcgaagcaa atccagtaag cggaacgatt gaagcggaca atacgtatgt
    47881 aaaaatcgaa ttagttggta aaggcgcaca tggtgcaagc ccacaatctg gtatcaatgc
    47941 aggttctttc ttggcattgt tcttagataa ctatgaattc ctaggatctg ctaaacaatt
    48001 tatccatgta gcagctgctt atgtccatga agatttttat ggagaaaaac taggagtcgc
    48061 ttatgaagat gaaaaaatgg gcaaattgac aatgaatgct ggactatttg cttttgaaga
    48121 aaatggtaca gaggaagcga acttcatcaa tatgaatttc cgttttccaa aaggtgtaac
    48181 agtagacgga ttgcaaagtg acatcgaaca aacagtgggg caagaaggcg caacagttac
    48241 tcgaggtgct cgcgtaatgg aaccacatta cgtaccaatg gatgatccat tggtggctac
    48301 attattgcaa gtctatgaag accacactgg agaaaaaggc tacgaacaaa tcatcggtgg
    48361 cggaacttac ggacgtttgt tgaaacgtgg cgttgcttat ggagcgatgt tcccaggata
    48421 tactgataca atgcatcaag caaacgaatt tatgagtctt gatgacttgt tcagagcaac
    48481 agcaatctat gcagatgcca tctatcgttt ggcgaaataa ttaattaaaa agaggatcaa
    48541 gaattgcaaa ttatttttgc agtcttgatc ctttttttac tttctactgt ggtattcatg
    48601 ataaacatga tatagattca attcatattc tttcttagcg ttcgtgacga ctgtatccaa
    48661 aatcaaccct gttactaatg aaagaagtgc taaagtcatc aatccagtac ttaaaatcgc
    48721 agaagggatg cgtgtaataa agcttgtcag catatattcg cgaatcacag gaatccccgt
    48781 gaccaatcca aataaaaaga agaagatggt ccaaatccca aagaaaagca aaggcttata
    48841 atctttaaac attttaataa tcatcatgat cactttgaat ccgtcagaga atgtattcaa
    48901 ttttgattcg ctgccttcag gacgatcacg ataatcgatt gggatttcaa cgaacttgaa
    48961 ttttttatct aaagcatgga tcgtcaattc cgtttcgatt tgaaaaccag aactcataat
    49021 aggaaaactt ttgacaaaga tacgattgaa tcctcgatag ccggtcatga catcaagaat
    49081 ttttgtttta tacattctag taattgtatt tctaacaagg ttatttccaa aatcgtggaa
    49141 aggtctctta ttctcatcaa agtaggttcc atttgatagg cgatcaccaa tgaccatatc
    49201 tgcttcacca gaacgtaatt tctcgagcaa accatgtaca gcttctgcag gataagtatc
    49261 atcgccatct accattaaat aatagtcggc atcgatatca aagaacattt ggcggatcac
    49321 atttcctttt ccttgacgag gttctttttt tacgattgct ccaccttgaa cggccaattc
    49381 gtatgttcga tcggtcgaat tattatcata gacataaata tctgcttctg gtaattctcg
    49441 tttaaagtct gcaatgactg ttgaaattgt tgcttcctca ttgtaacaag gaatcagaac
    49501 agcaattttt tcttgttgtt ccatttttca ctctccttaa ctatagaaaa caaaagctaa
    49561 attaaaaata cctcataatc aaaggaaatg ctagtttttc ccgctaaata tcatttattt
    49621 aaaaagaaaa tactggaata tatgtttgga aagctctatc taaaaaacca cccactacaa
    49681 aagaaggaca tgatacctct cttttgaata gtgggtgcgt attcgttctc tcactttttt
    49741 gacgtattaa tctgttgcac ctttttttct tgaaatttct aatacggttc ctgtatgagc
    49801 atctgctaaa aattcataat aaacagtttc gccatcttct attcgagaaa gacctccccg
    49861 ataagcactc gaccgaacgg cgaatttcct aataggttcc ttttcaaaag agatccaaga
    49921 accttcgatt gcaccttctt ttagaaaaga tgacttgata ttttcaagta cttggtctgc
    49981 tgacaatact tttttgcgat gataccaaat cgttgaagct accccgctca ataatcctag
    50041 accgattcca actgctaacc cgcctttaaa atagcttagt tcgctttctt catacatgtg
    50101 gacatacctc cttcttctca cttgttactc cttgtgctta ttttaccaca ctctcatatt
    50161 ttctttgaaa taaataaccg ctaccagaaa aaataagcta taataaatac gatcggtctg
    50221 cagaaaaaat catattttat ccgcagatca atataagtct gagaggagtt tagtctgaaa
    50281 tttagacgaa tagcatatgg aagaaaaaac atttcaacga atcaaagagt taacagaatt
    50341 acaaggaaca agcggttttg aacaagatgt tcgcgcttat atggaaagtc atatggaacc
    50401 gctggtagat gagttacagt tagatggttt gggcggcatt tttggtctgc gccatcatga
    50461 agaagcggat gcaccccgtg taatggtcgc agcacatatg gatgaagtcg gattcatgct
    50521 gacacaaatc caagaaaacg ggctttttaa agtcgtacct ctaggcggct ggaatcctta
    50581 cgttgtttct gctcagcgtt ttacacttaa aacctctact ggtaagaatt atccatgtat
    50641 ttcttcttcc gttcccccac atttattgcg aggaacaagc ggacaaaaat cagtagaagt
    50701 aaccgatatt ttgttcgatg ctgggtttga atcaagagaa gaggcaatga gttacggtgt
    50761 ccttccagga gacacaatcg ttccttacgc tgaaacgatc aaaacagcca acggaaagaa
    50821 tattatcagt aaatcatggg acaaccgcta tggctgtaca atggtattag aagctttaga
    50881 agcgttgcaa aatgaaacat taggtcatac attgattgca ggcgcgaatg tccaggaaga
    50941 agtaggcttg cgtggttcta aagcttctgt caataaattc aagccagatc tgttctttgc
    51001 agtagactgc tctgcagcag atgatacagt gacgaaaaat ggcacattcg gacatttagg
    51061 agaaggtaca ttgatgcgta tccaagatcc aggtttgatc atgcttcctc gtttgcgcga
    51121 gtacttgctg gatatcgctg aaacaaacaa tatcccttat caatactttg tttcaaaagg
    51181 cggtacagat gcaggtgctg ctcatacaca aaacgaagga atcccaagta cagtgattgg
    51241 cgtagtggga cgttatatcc atacgcatca aacgatgttc agtattcgcg attttgaagc
    51301 tgcacgagaa atgttgattc aaacactgaa aggtttggat aaatcaacag taaatacgat
    51361 cgtatacgga aaataatgcg atagtgaaac tataaaatct ggcaaaagat tgctttttgc
    51421 cagatttttt ttataaaaag aggatcaatg tttgttagtt cgaaagaaag gtgtataatt
    51481 taattgaaaa atacataaac agaaagtaga ggacaaagaa tgattatccc aaatagttta
    51541 gaagaattag caggctacgt agaaactgga aagagtgtat ttttctttac tgccggctgg
    51601 tgcggagact gccgttttat caaaccgcaa atgcctgaaa ttgaaaatga cttttctgat
    51661 tggcaattta ttgaagtaga ccgtgacaag tacatagatg tagccgctga atggaacatt
    51721 tttggtatcc caagttttgt cgtgatccaa gacggaaaag aattaggtcg tctagtgaat
    51781 aaagatcgta aaacaaaaca ggaaatcgaa tcgttcctag aaagtgtaac aaaaggctga
    51841 catctgttag gaggggtcaa gatgaatcaa gaatataaga atattttagt aggtatcgac
    51901 ggtagtgaac aagcacttgc tgctttcaaa aaagcggtag aagtggctcg ccgtaataat
    51961 gggaaagtat atgtagcaaa cgtaatcgat cagcaatttt ataactttat gggctatgca
    52021 cctattgatc aaaatctagt cgatcagcaa actgaaacag ccaaggaaat gatcgaagaa
    52081 tgtaaagaat atgggaaatc tgttgattac acaaatatag aaggaattat tgcatatggt
    52141 tctgttcgtg aatccatggc agtcaaattg ccaaaaaaat atgagatcga tctaatcatg
    52201 gttgggcagt caggtctaaa tgcagtcgaa cgctttatga caggaagtgt agccagctac
    52261 gtgatacgtc attcaccttg tgatgtcttg atcgtttctg acgaagaaga acagccaagt
    52321 aattaagcaa gtggaacttg tacgaccttt ttaatcaatt aactaaaaca gtaaagggac
    52381 tgtgacaaaa gtcttgtcac agtcccttgt tacgaataaa tggtgttcca gaagaggctt
    52441 cttcggaaat aaagcatgaa aaaaatttca tgcagtgaat gcaatttttt gttaagataa
    52501 atctcagcaa aggataatgg aggaaagaac atgatttttg cttataacaa agcacacgtc
    52561 ggagacacct tgatggttat tatagccgac gataaaggaa cagaaaacac tgttgaacgc
    52621 aaatggaatg ttgctcggat caaagatgaa caaggtcaag ttgttgcttg gaactttttc
    52681 catatctccg atcatctaac aatcgaaggg aacggccaag taagcttaac agatgaggaa
    52741 ttagcgaaat tgaatcgatt gatcaaagaa gcaggattcg aagaacagct tgaagctgat
    52801 catgaaccga agatcgttgt cgggtacgta aaaacatgcg tcccacatcc tgattctgac
    52861 catttatcga ttactgagac agaagtagac aatggtacag tattgcaaat cgtttgcggt
    52921 gcacccaata tcgaagctgg acaaaaagtt gtcgttgcaa aaccaggagc tatgatgcca
    52981 gatggaatga tgatctggcc aggaactttg cgaggggtag atagctatgg aatgatctgt
    53041 tccgctcgtg aattgcattt gccaaatgct ccagcaaaaa aaggaatctt agtattgcct
    53101 gaggatgccg tagtcggaga agcatttcca aaataaagag gttgtgacaa gtctttgtca
    53161 caacctcttt tttattaatt ttcactattt tgattttgtt ttaatgccga ttgatcgatc
    53221 gtcaattcaa cagtagttgt tttttctttc gagccttcat agtaagtgat tttgacagaa
    53281 tctcccactt ttttcttata caaagcagat tgaagttcta caccagaaga aacttctttg
    53341 tcatcgatct tagtaatgac gtcgtattgt ttcaatccag ctttttcagc cggtgtagct
    53401 gtttggacat ttgtcacgat gacaccgtta gttacagatg aaggaatctt caagatttgt
    53461 tcttgttgct gagtagaaac ggcagataag tcgaccattg tgatacctag agctggacgt
    53521 gtcacttttc cgtctttttc cagttgattg atgatattca ctacatcgtt acttggaata
    53581 gcaaatccca ttccttctac gcttacatta gaagtagatt cagaagtact tgcgatctta
    53641 cttgagttga taccgataac ttgtccttcg atattaacta aaggaccacc agaatttcca
    53701 gggttgattg ctgcatctgt ttggatcgca ttgatattga ctgtttcgtt cgattcattc
    53761 gtacttgtca cttgtcggtt caaggaagaa atgattcctg aagtgacaga atttgcatat
    53821 tcagatccta gtggggaacc aatcgcaatc gcaggctcac caaccttcag agcacttgag
    53881 tcaccaaaag aggcaactgt ttcgactttg tctgaattga tcttcaacac cgctaaatct
    53941 gaataagcat ctgttccaac taattcagct tttaccttcg taccgtcttt catcaagact
    54001 tcaagacctt gctggccatc tactacgtga ttattcgtga ctacataagc agtattcccg
    54061 tcttttttgt agatcacgcc gcttccttca ctataagctt ccaaattact atcatctgag
    54121 ctgctacttt cttcttgctg cccgaatagt ccgccaaaac cgcttgattg gttttgactt
    54181 tgtaagttga taacagaaac gacagcgtct tgaactttat ctaccgcttt ggtgatatca
    54241 gaatctacat tgactttgac attttccaca actgtttcac cggcagaatt ctgattgccg
    54301 cttgtagcag tagaactatt gttgccagtg cccatcgctg cataaaaaat gccagctgtc
    54361 agtagtcctc caacgatccc tccaacaagc cccagaccta acttacgcca gaggctatta
    54421 tttttacttt ttttcatctt tggtgtcaca ttttttctat ccatgtgttc ctcctccaat
    54481 tacattcatt tatctactgt tagtatagtc ttatttgttg actttagtct actttgagac
    54541 tgtgaacttt ttatgaaaaa ctttaggaaa aatcattgtt ttcagatgaa aatatttgat
    54601 tttctttttc cacagatact gtgtaaaact acgcggatag gattggaaaa ataagggata
    54661 aaacatatcc acagcctgtg tataaagtgg ataaattctt ataaaacgta tgtttgccta
    54721 atgaatgtga tgaaataagg taaaataaca ctgttttctg tggataaacc tgtggatgtt
    54781 gtgcataact gttggggaaa tctttttaac aaaaatagcc aaaataaaca tatagacgaa
    54841 aaaattgatt ataataaaca aagtgagcat gaagaccaaa ggagagaaaa catgaatatc
    54901 aaaattatct cagtaggaaa attaaaagaa aaatacctga tccaaggtat caatgagtat
    54961 gtcaaacggc tgaatgccta tgcaaaaatc gaattgatcg aagttcctga cgaaaaagcc
    55021 ccggagaatt taagcgaagc gcaaatgaga caagtaaaag aaaaagaagg ggaacggatc
    55081 ttagcgaaaa tcaaagaaca agaatacgtc tatgccttag caattgaagg gaaaaatcct
    55141 acaagtgaat cttttgctaa acaaatcgac caattaggta tccaaggaaa aagccacctt
    55201 gtttttgtta tcggcggctc tttaggactc agcgaagccg tcatgaaaag aagcaatgct
    55261 cagatctctt ttggaaaaat gacgtatcca catcaattga tgcgtctgat ccttgtcgag
    55321 caaatctacc gtgccttccg gatcaatgcg ggggctccat accacaaata atgatatttt
    55381 ttaagagaaa aaattatccc tattttaaat aatagcaata ggtagtggta gggagaaatt
    55441 tataaataac ttttttattt gctttggtag ccaagtaagt tgcaaaagaa aaatagatac
    55501 tgttaaaaag ataatgtaga catagaaaca aatacgtaaa attttagtta gaataatggc
    55561 aaggagaaaa aacatgatag atgaaaaaaa gtgtttaaaa aatctattag aggatatgga
    55621 tgatattgaa gaaaactatc taaatggact gggaaaacaa atatatttca gatacttaag
    55681 ctatctaaaa atgaaataag acatagtaat tttttagctt ttttgtttgc tccaaatgaa
    55741 acccacaatt tatctgatga gttttttaaa atgtttttga aaaggtatat agattctaat
    55801 gatgatacca aagctgctat caattatttt gatgctttac ttaatagcta tgaagattta
    55861 attgtttaca gagaaaataa gaatattgat attttattgg tatcggaaaa aaataaaata
    55921 gtggtgtgca tcgaaaataa aattctgtct tcggagtctg gagggcaact taataaatac
    55981 caaatatata tagaacgcaa ttattctaac tataaaaaaa tttttgtttt tttaactcca
    56041 gatggaaatg aacccacaaa tcctagttgg ggcataatta cttacaagaa tatcgttga
//
LOCUS       DAYYCW010000008        53863 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000008 DAYYCW010000000
VERSION     DAYYCW010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.008"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            883..1719
                     /locus_tag="ACHAYA_000467"
     CDS             883..1719
                     /locus_tag="ACHAYA_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFM8604299.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            1783..2214
                     /locus_tag="ACHAYA_000468"
     CDS             1783..2214
                     /locus_tag="ACHAYA_000468"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8604300.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(2276..3598)
                     /locus_tag="ACHAYA_000469"
     CDS             complement(2276..3598)
                     /locus_tag="ACHAYA_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM8604301.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3752..5392)
                     /locus_tag="ACHAYA_000470"
     CDS             complement(3752..5392)
                     /locus_tag="ACHAYA_000470"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM8604302.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            5725..6195
                     /locus_tag="ACHAYA_000471"
     CDS             5725..6195
                     /locus_tag="ACHAYA_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM8604303.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            6417..7520
                     /locus_tag="ACHAYA_000472"
     CDS             6417..7520
                     /locus_tag="ACHAYA_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM8604304.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            7804..8484
                     /locus_tag="ACHAYA_000473"
     CDS             7804..8484
                     /locus_tag="ACHAYA_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8604305.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            8574..9608
                     /locus_tag="ACHAYA_000474"
     CDS             8574..9608
                     /locus_tag="ACHAYA_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8604306.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            9622..9957
                     /locus_tag="ACHAYA_000475"
     CDS             9622..9957
                     /locus_tag="ACHAYA_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604307.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            9941..12421
                     /locus_tag="ACHAYA_000476"
     CDS             9941..12421
                     /locus_tag="ACHAYA_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604308.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            12477..14036
                     /locus_tag="ACHAYA_000477"
     CDS             12477..14036
                     /locus_tag="ACHAYA_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8604309.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            14033..15397
                     /locus_tag="ACHAYA_000478"
     CDS             14033..15397
                     /locus_tag="ACHAYA_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8604310.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            15568..16638
                     /locus_tag="ACHAYA_000479"
     CDS             15568..16638
                     /locus_tag="ACHAYA_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8604311.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            16653..17321
                     /locus_tag="ACHAYA_000480"
     CDS             16653..17321
                     /locus_tag="ACHAYA_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8604312.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            17341..17919
                     /locus_tag="ACHAYA_000481"
     CDS             17341..17919
                     /locus_tag="ACHAYA_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8604313.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            18320..19231
                     /locus_tag="ACHAYA_000482"
     CDS             18320..19231
                     /locus_tag="ACHAYA_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8604314.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(19609..20526)
                     /gene="rnhC"
                     /locus_tag="ACHAYA_000483"
     CDS             complement(19609..20526)
                     /gene="rnhC"
                     /locus_tag="ACHAYA_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM8604315.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            20728..21165
                     /gene="zapA"
                     /locus_tag="ACHAYA_000484"
     CDS             20728..21165
                     /gene="zapA"
                     /locus_tag="ACHAYA_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM8604316.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            21223..21771
                     /locus_tag="ACHAYA_000485"
     CDS             21223..21771
                     /locus_tag="ACHAYA_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFM8604317.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            21886..24246
                     /locus_tag="ACHAYA_000486"
     CDS             21886..24246
                     /locus_tag="ACHAYA_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM8604318.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            24333..24647
                     /gene="trxA"
                     /locus_tag="ACHAYA_000487"
     CDS             24333..24647
                     /gene="trxA"
                     /locus_tag="ACHAYA_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM8604319.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            24829..26607
                     /gene="uvrC"
                     /locus_tag="ACHAYA_000488"
     CDS             24829..26607
                     /gene="uvrC"
                     /locus_tag="ACHAYA_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM8604320.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            26752..27651
                     /locus_tag="ACHAYA_000489"
     CDS             26752..27651
                     /locus_tag="ACHAYA_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8604321.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            27644..28906
                     /locus_tag="ACHAYA_000490"
     CDS             27644..28906
                     /locus_tag="ACHAYA_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8604322.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            29067..30965
                     /locus_tag="ACHAYA_000491"
     CDS             29067..30965
                     /locus_tag="ACHAYA_000491"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM8604323.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(31295..32014)
                     /locus_tag="ACHAYA_000492"
     CDS             complement(31295..32014)
                     /locus_tag="ACHAYA_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM8604324.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    32204..32251
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            32351..32683
                     /locus_tag="ACHAYA_000493"
     CDS             32351..32683
                     /locus_tag="ACHAYA_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604325.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            32670..34673
                     /locus_tag="ACHAYA_000494"
     CDS             32670..34673
                     /locus_tag="ACHAYA_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM8604326.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            34692..35162
                     /locus_tag="ACHAYA_000495"
     CDS             34692..35162
                     /locus_tag="ACHAYA_000495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM8604327.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            35183..35767
                     /locus_tag="ACHAYA_000496"
     CDS             35183..35767
                     /locus_tag="ACHAYA_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFM8604328.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            35782..36768
                     /locus_tag="ACHAYA_000497"
     CDS             35782..36768
                     /locus_tag="ACHAYA_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM8604329.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            36758..37069
                     /locus_tag="ACHAYA_000498"
     CDS             36758..37069
                     /locus_tag="ACHAYA_000498"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM8604330.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            37091..38872
                     /locus_tag="ACHAYA_000499"
     CDS             37091..38872
                     /locus_tag="ACHAYA_000499"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM8604331.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            38865..40241
                     /locus_tag="ACHAYA_000500"
     CDS             38865..40241
                     /locus_tag="ACHAYA_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM8604332.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            40248..40883
                     /locus_tag="ACHAYA_000501"
     CDS             40248..40883
                     /locus_tag="ACHAYA_000501"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM8604333.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            40913..42268
                     /locus_tag="ACHAYA_000502"
     CDS             40913..42268
                     /locus_tag="ACHAYA_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8604334.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(42460..43854)
                     /locus_tag="ACHAYA_000503"
     CDS             complement(42460..43854)
                     /locus_tag="ACHAYA_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8604335.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            43946..44917
                     /locus_tag="ACHAYA_000504"
     CDS             43946..44917
                     /locus_tag="ACHAYA_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8604336.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            44914..45696
                     /locus_tag="ACHAYA_000505"
     CDS             44914..45696
                     /locus_tag="ACHAYA_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM8604337.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(45865..46236)
                     /locus_tag="ACHAYA_000506"
     CDS             complement(45865..46236)
                     /locus_tag="ACHAYA_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8604338.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            46370..46813
                     /locus_tag="ACHAYA_000507"
     CDS             46370..46813
                     /locus_tag="ACHAYA_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8604339.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(46856..47665)
                     /gene="yidA"
                     /locus_tag="ACHAYA_000508"
     CDS             complement(46856..47665)
                     /gene="yidA"
                     /locus_tag="ACHAYA_000508"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM8604340.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(47684..49051)
                     /locus_tag="ACHAYA_000509"
     CDS             complement(47684..49051)
                     /locus_tag="ACHAYA_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8604341.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(49035..49865)
                     /locus_tag="ACHAYA_000510"
     CDS             complement(49035..49865)
                     /locus_tag="ACHAYA_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM8604342.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            50028..50465
                     /locus_tag="ACHAYA_000511"
     CDS             50028..50465
                     /locus_tag="ACHAYA_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM8604343.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            50541..51161
                     /gene="rpoE"
                     /locus_tag="ACHAYA_000512"
     CDS             50541..51161
                     /gene="rpoE"
                     /locus_tag="ACHAYA_000512"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM8604344.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            51350..51856
                     /locus_tag="ACHAYA_000513"
     CDS             51350..51856
                     /locus_tag="ACHAYA_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8604345.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            51873..52772
                     /locus_tag="ACHAYA_000514"
     CDS             51873..52772
                     /locus_tag="ACHAYA_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM8604346.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            52859..53743
                     /locus_tag="ACHAYA_000515"
     CDS             52859..53743
                     /locus_tag="ACHAYA_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8604347.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT        17664 a         9352 c        11320 g        15527 t
ORIGIN      
        1 gactttataa gtccagagtc aaatctattt acgttatctt gggaaagaaa acattcgtcg
       61 atgcaaaaag tgacgtttga aaaagtggat tcaaattatg tagctccact cattgatttt
      121 aaagaacaag aagaaattta aaaaccccca cggcttattg aagtttttgt atgccatatt
      181 gtatgccatt tggtttaaat cgcttgaaaa atagtgatag ataactctaa ttcatttagt
      241 cataaacgct gttttgataa cctttatagt tctttgatat gggtttctca ttcatggcgg
      301 aagaagaaga atagaacaaa agaatgaatt aataagatat aaaaaaaggg attgtgacaa
      361 acgtcttgtc acaatccctt tttccaaata aacgatgttc aatcagctgc ccctcggcat
      421 tcgctcgaaa aacggcgaaa tatgaagaac tattttgcca tttgtttctc taataatcag
      481 gtcaaaacgt tgacttcaca gtctcttcct taaacggtgt tctcctgcca actcctcggt
      541 cacaagtcac aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact
      601 cggagctgaa cggcaggctc acgacctctt tcttaaacgg tgttcaaaag cttgcatctg
      661 cggtaataag ttcaaccaaa cgaaaattat gaggagctat ttttgtttgg ctgttcttat
      721 tacttgatgc aggacagctt tttcacaacc tcttttcgtt tagcactaaa tttccttaaa
      781 aagtagaaaa gctttcgaag cctcgtttgt ttcttttact gtataataaa agaaaagagt
      841 aacaatcgct aaaagatata aacaagagaa aggaaataca agatgattgg tctgatttat
      901 tttgtggtga tcgttctggc gaacacagtt ggcgctattt ccgggatggg tggaggcgtg
      961 ttgatcaaac cgattcttga tcttatcggc gcccattctg ttgcagggat ttctttttac
     1021 tcaacagttg cggtattcac gatgtccatt gtttcgactg tgcgacaagt atcaagcggg
     1081 aaatcactga attggcagat tgtcggttgg gtatctagtg gagctgttgt tggaggagta
     1141 gtcggcaaca ttgtattcga agttttttta cagttatttg aaaacgagaa gcatgtgcag
     1201 ctgatccaaa tttttttgac ggtattgacc ttggtttttg cgttcttcta caccaagcat
     1261 catcagccaa aatttcattt gacgtcatgg acttggtacc ttatatgtgg gggggttctg
     1321 ggctttttag ctagtttttt aggtattggt ggtggaccag tcaatgtttc tttgttgatg
     1381 ctgatgtttg cattgccaat aaaagaagca acactatatt ctctatctac gattttcttt
     1441 tctcaactgg caaaattggt aacgattgct ttaacaagta gctttatgcg ctttgattta
     1501 tctatgttgt tttatgttat ccccgctgct gttattggag gtttgtgggg agcaagattt
     1561 agcagggttt tgtctccaaa aaaagtgacc tttatttttc aagtaattgt cctcgtggtt
     1621 cttttgatca atctttacaa tgctttcgtc atcttacaaa cattaggaga atctagacta
     1681 acgcaagtca aaatcactga aacatattgt tctaaatgaa aaaaggagta tactgtgata
     1741 aaaaaattca tttaaaaaaa taaaaattgg ctggtgattt ggatgttgcg ggaaatgcgt
     1801 ttaggagatg cagaagcact acgagaaatc aatgcggaac aattaggata tgatgtacca
     1861 ttaaccgtta cggtgagaca aatgaagaaa ctgttgaagg agccagaaaa aaattttttc
     1921 ttggtctatg aagatcctac ttctaaaaaa gcagctggct atgtacaagc ggagtcctat
     1981 gaatcgattt tcagcgatcc gatgttcaat attatggcac ttgctgtgtc taaacatgct
     2041 gaaaaaagag gaatcggaaa ggccttgatg actggcgtag aagaagcgaa aagacgagga
     2101 atatcagcaa taagacttaa ttctgcagaa tacagagtag aagcacaccg attttatgaa
     2161 cgcatcggtt atcattccga taaaatgcaa aaaagatttt tgaaaatcct gtaaattttt
     2221 atcaaaaaat acctggtcat caagccgata cttgatgatc aggtattttt tttgactaag
     2281 aaagtaattg catcaagaaa cctgagatga tcaaaacgat ggcaccacca agccgattgc
     2341 ccatttgagc aaaagcaata agttccatcc gattagctgc agataagaca gctacatttc
     2401 ctgtaccacc catcgagttg ttgcataagc cagcagtaat agttgattcg attggatata
     2461 aaccaaacaa acgaccgaca aatcctgaaa ctactgaaat cgcgataaca cttgtcaaac
     2521 ataagacaac aaattgccat gtcaaagcag aagccaatac atttagattc aataacgcaa
     2581 taccgatacc ggcaagaaca gctgctgtca agtttttgac aatcaagttg ttgaacatga
     2641 tagctgcatc ttcgtaatat tctggtaata ggtttgcgat tttacagata acaacgataa
     2701 tgatcatgaa tgcgtaagca tggactttag gaacaaaata gttacagatt tgtcctaaga
     2761 tgaagaaagt caatgatacc atcaaaccga cacctaattg tggaatggaa ggttttacat
     2821 ttctaggttt tccatcgcca aggtcgcctt tttccattaa tttaccatta ccgttgaatt
     2881 ttggcattga ttgtcccgca cgagcaacta acgctgcacc aatgatagct aggacatttg
     2941 tcattgctgt cgcaggaatc aggcgggaaa tgatagaaga agcgtccgta cctaaaacat
     3001 ttgcataaat cgttgataaa ggtacagctc cggctccaac accaccagcc attgcgggca
     3061 atgagatata taaaacggaa tttgcaaaac catttccgat aagcatacct acgagtccta
     3121 cggcaaaaaa ggagacgacc attgaaataa gtgcaacggg aataaaacga acagaagctt
     3181 tcatcaatag attacggttc ataccaagga tgctaccagt aatcaaggca gcaatataaa
     3241 agtctaaaaa gcccatattg ctgacaaagt tttctgtgga ttttactaca tactcaggta
     3301 agatcccaaa tgtagcaatc gcggcagagg caaagatcgc aaaaacggag ccaccaccta
     3361 aataactttt gacgataggg atttttgttc cgataaaatg aaataaatta ccgaaaatga
     3421 ccaaaacagc gattgggcca atcatattcg tcggtaattt tcctaaagcc attgcaatga
     3481 tcaggacaag gaccatgatc aaataaaggg gcaaacttac gccggtaatt tttgttgacc
     3541 aaaacccttc ttttttcttt tcctcaactg cagttgcggt tgttgttttt tgtgccattt
     3601 ttacagatcc tttcttattc tgtatcgtat gaaaaaagcc aaagagaagc ggcaaaagag
     3661 cagtagttgg ctggctgctg ttttgccgca gtcgcttttt tctttttaga cgtcatacaa
     3721 agtttctgta tgttctttta ctataacaat cttaatcttc taaagattta tattcaggaa
     3781 cccatttagc agattcaaca gcttctttga tatccgtgat ttcttcacga ttcaattttt
     3841 gtgcaacaac tgattttgcg acagtttcag caatcgtttg agagaattca gtgatttttg
     3901 ctactggagg caagatagct gctcctggtt ttgttacatc aacgattccg cctaatgctc
     3961 gactagcttg agaaatgatt tcagcattta ctcgtgtagc tgttgaagca atcaaaccaa
     4021 gccctaaacc tgggtacatc aacgcattat ttgcttgacc gatttggtaa gttacaccgt
     4081 tatattccac atcagcagca gggatacctg ttccaactaa agcttttccg tccgtccatt
     4141 caatcaaatc ttttgctttt gcttctgcca attttgttgg attagacaaa ggcatgatga
     4201 ttggacgtgg ggtatgagcc gccatttctt tcacgatttc ttcagtgaat gctccaggtt
     4261 gggtagaagt accgatcatg atcgttggat ggattgcttt tacaactgct tctaagtttg
     4321 tcaattcatc agcattagtg aattctgagc gtttacgagc gaatgggatt tgaccaggag
     4381 tcaatccttt tgtatcttca aacaataaac cctgtttatc tacttgatag aaatgttggc
     4441 gtgcttcttc ttcaggtaca ccttgacgga tcatttcatc taaaaggatg tttgcgatac
     4501 ctacaccagc agtaccagca ccaaatgtca agatcgtttg atctttcaat tgctcacctg
     4561 atatattcaa tccacctaat acaccagcta gtacaacgat tcctgtgcct tggatatcat
     4621 cattgaaagt agtgatttta tcttcgtatt tttctaagat cgttgcagca tttcctcgtc
     4681 caaaatcttc ccaatgtaat aacaattctg ggaataattc agtagctgat tgaacgaatt
     4741 gatcaatgaa ttcgtagtaa gtttcgcctt ctacacgagc atgacgattc cctaagtata
     4801 atggattatt caataattct tcgttattag taccagcgtc gattgataca ggcaagactt
     4861 gtgcaggatt gataccagcc gcagcagtgt agaccatcaa tttaccgata gcgatgtcaa
     4921 caccgttcac accccagtca cccataccta agattccttc tgcatctgtc acaacgatca
     4981 aacgaatgtc gcgtccatct gctgcgtttt tcaaacttgc tttgatattt tctggttcgt
     5041 cgattgaaag aaatgcagca tcttgtggtt tcaagaaaat ttcgttgtat tgttcgattg
     5101 aatccgcaac gacaggatca taaacgacag gcatgaattc aactaaatgt tcgcccatca
     5161 atttataaaa caatgtccgg ttcgtattga atagattcat taagaagata cgtttttcca
     5221 aatcggtctg tttgcttaag tattgaccgt aagcttggac agattgttgt tccaatgttt
     5281 ggacagtact tggaagcata ccagtgattc cgtattttgc ccgttcttct tttgtaaaag
     5341 ctgttccttt atttagaaaa ggattattta ataagtttag ccctgtaagc atgaatgatt
     5401 cctcctaagt tttaatacaa acgaagtata ggagtcagat gaggatatag tcaaaaagag
     5461 caggtataat aaattttatt tatatgagta atagagtcaa aagaaatcaa aatagcggta
     5521 tttgttcttt ttatatcaac aatccttgct tgattactat gcgtatctct tatatgctaa
     5581 agaaagaaaa gtagaagaat aaaggatatg ttagatagat aaatagaatc tctgaagaat
     5641 aaatcacgtg atcgtgacga taaaatacac ttacgtcagg tgataaaaaa tacataagta
     5701 gaaaactaag aggaggaaca gaaaatgaaa gaagttcaat tgatcagaaa aagcgaacta
     5761 tcagaaggtg gttgcaatgc atgcggcgta gtagaagcaa caagctatac attaaagctt
     5821 ggtgccaaca aagcgataat ctctgagttg acagtaggcg gtcttgtgga cagtctggcg
     5881 cttgctgaag gttttattgg agaagatatt tatgagatgt tttcagaaat ccgtcagtta
     5941 aaaaaagggg aaaactgcat agaggttcat catgagagcc cgaatgtgag attcaaacgt
     6001 ggtgataatg agatgatctt caataatcat gtgtccgatc acaccgagct atatggaatc
     6061 gtgaatcaaa tactaactga gctttttgga ttgggacctt atgcgttcaa ggaagaaaac
     6121 ggtaatccaa aattaaacga agaatggcaa gaaacgattg aaatacaaag aaacaacccg
     6181 cacttatttc aataggcatc cacagtattt cgtaaaaggg actttgacac gacttttgtc
     6241 aaagtcccat cgttttttat ctatttgtgt tctaaacatt tctttcagag tagtaacaac
     6301 tggaatgact gaaatgtaaa cagattattt gcctgtatca atacggaaga gtaatataaa
     6361 gtgtgtaaac gctatcatat tattcattca attttttata gagggaaggc attgaaatgg
     6421 aaaaaacgta tcaagcaggt acaaatgaag gaatagtgga ttttatcaat atggaagatt
     6481 tagaaatagc cgcttcacaa gtgattccag ctggtggtta tggttacatc agcagtggtg
     6541 ctggcgactt atttacttat caagaaaatg aacgggcatt caatcatcag ctgattattc
     6601 ctcatgtatt aaaagatgta gaattacccg atacaactac acattttgac aaagaaacgc
     6661 tgactgcacc aatcatcatg gcaccagttg ctgctcatgg cttagctcat gtgaaggcgg
     6721 agaaagcttc tgcaaaaggt gtagcagatt ttgggacgat ctatacagca agttcttacg
     6781 cttcttgtac attagaagag ataagagaag caggcgggga gaaagcaccg caatggtttc
     6841 aattttatat gagcaaagat gatgggatca atttggatat tcttgaagtg gccaagcgta
     6901 atggagcaaa agcaatcgta ttgacagcgg atgcaacagt tggcggaaac cgtgaaacag
     6961 accgcagaaa tgggtttacc ttcccactgc caatgccgat cgttcaggct tatcaatcag
     7021 gagtaggcca aacgatggat gctgtgtaca aatcttccaa acagaagctt agtccaaaag
     7081 atgtagaatt tattgctgct cattctgatt tgccagtcta tgtgaaaggt gtccagtcag
     7141 aagaagatgt ctatcgttca ttagaatctg gtgctggcgg aatctgggta tctaatcatg
     7201 gtggccgtca actagatggt ggtccagcag cgtttgattc gttgcaatat gtggcagaag
     7261 cagtagataa acgtgtacca atcgtgtttg acagcggtgt acgacgcgga cagcatgttt
     7321 tcaaagcaat cgcatcaggt gcagatcttg ttgctattgg cagacccgtt atttatggat
     7381 tgtctttagg aggaagtaca ggagtccatc aagtgtttga tttctttaaa acagaattgg
     7441 aaatggtcat gcaattagct ggaacacaaa cagtagaaga tatcaaaaaa ataaaattgc
     7501 gtgagaatcg ttttatttaa gaaacaaatg acgaaaaaaa taggagtgcg gcagatgatg
     7561 ccgtactcct atttttatag ttcaaggatg tcactaatag aataatttga aacgaaaaaa
     7621 taaaattgaa atattttttc atacttaata aaaaaggaag aatattcatg taaacgattt
     7681 tattgttttt ttctctttta tacaaaaagg gtcataattt caagtaagcg gatgaattca
     7741 tgaggatttt ttgcatactg tctctactat acttaggtca ctaagaaaaa ggagtgattt
     7801 attatgaaaa agaacatgat tgggacatta ttactttcaa cacttcttct aggcggcttg
     7861 gcaacaccag cttttgctga gggtcaagcg acttcaaaag gagatattac ctttactgaa
     7921 ccaacaaata ccgtggagcc tcttaaccct actgatcctt caaaaccagt agagccagca
     7981 gatccggaaa atccagcaac tggccagaca ggtcctttaa cactggatgt agttcccgaa
     8041 ttgccatttg gtacacacga gattgaaagc ggaacaaaaa cgtatcaagt agacgcttca
     8101 aaaaacgata caccttatct ccaagtatca gatcgtcgag gtgtaggtgc agatggccaa
     8161 gcacaagggt ggaacgtgac agtttcggtt tcagattttg taaacggttc acaagttttg
     8221 caaggcgctg aattagattt tggaacatcc actgttaaaa gtacgtcaga taacgaatcc
     8281 actggaccaa ctagtcaaac tgtaactggg ttgagtaaag caagtgcagc gacaccaata
     8341 tttacagcag caaaagatca aggtctggga acatggcttt ctgtttatga tccagcaaat
     8401 atcacactga aagtgccgaa agcagcagct ggaacattca cggctgattt gacttggaac
     8461 ctcgtggctg gcccagtagc atagagtatg aaataaattc aacaaactgc ggtgaaaata
     8521 ctataggtga aggatttttt accgcagttt ttgttgaaag gatgtggtgg acaatgaatc
     8581 aaagtaaacg actgtttgtt tcgttttttt ctattttaag tctatttttc atttttccgt
     8641 caatcagcca agcaaaggat tcagcaggag actttggaat caaaccggta tttccagaga
     8701 accaaataga taaagcaatc ggttattttg atttattagt ggcaccggaa caaaatcaga
     8761 cactagaagt aattatcagc aattcttcag atgaagaacg aacatttgag gtctctgtca
     8821 atccggcggt aacgagtgat ggtggaacga tcgattatag tcaaaaaaac ccaacgttag
     8881 atgagacatt gccgtttgat gtccgtgacg ttttgctcat tgcgaaaaag gaaatcaatg
     8941 tatcagcaca tgcagaaaca actgtaccta tagaagtgaa aatcccagca aaaagcttta
     9001 agggacgtgt attagcgggt attcatgttt cacctaaaga agaagctgaa acagaaaatg
     9061 ctacagaagg agcacaaatc aaaaatcgta ttgcttataa tctcgcagtc gttttacaag
     9121 aaagtcaaga gacgatagaa ccggatctga aacttttatc aggagatttg gacgaggtca
     9181 acgcaaagcc aactgttcag cttcgtttcc aaaatcctca gcctaggatt atcagcaatt
     9241 tgatatttac gagcaagatc ttttatgaaa atcagcttta tattgaaaat acgtctaatg
     9301 catttttagt tgcaccaaat tcgaattttc acttgaatct tgatttagct ggtgataaag
     9361 caaaagccgg cgattaccgc gcagaaatca tagcaaaatc aggcgacagt aatgaatggc
     9421 gatttacaca aaactttacg atcaaaaaag aaaaagcaca aaaagtaaat gaaaattccg
     9481 tttttgcagt acaagaacaa tctttcccat ggctttatgc aggattggtt gctgcagttc
     9541 tagtgttatg gttgattttg ctgtttttgt gggtccacaa gaaaaagaaa aaaggagaaa
     9601 caacatgatg aggtgatttc gttgaaaaca tcgatcttat tgggagggat tattgcaagt
     9661 ttgctgttct tcagctggca agagccagtc caagcagata ccaaaagttc aggtacgatt
     9721 acattttatg atctaagtcc aattccgcat ccaagtggtt cagtcaatgg cgcatcaaaa
     9781 cagcagacac agctaccgat ttataaagaa tctaatcaac atggcgtatt cccggagacg
     9841 aatgaacaaa agatccgttt tctttatttg atcattggta tagaattatg gcttcttgcg
     9901 tggttgttgt tatggagaag aaaggaaaag gaggatgaac atgttgctac aaaataaatt
     9961 ttcatttttt cgctccttct tttttacttc gctttttgtt ttaagtatat ttatcttttc
    10021 accttcggca gaagctgttg gagcaacgcc tcctgccatt tcttatgatc catacacgta
    10081 tgaaaatatc gtacctccaa atggtggacg tagtgcagtc aatggcggga agcttgtcca
    10141 actaactggg gctaaccaat tgcctaaatt gactgctact tatttgaacg atggttcaaa
    10201 tgatccttgg aaaggtggca cggtccgttt tcattgggaa agccatgtga aactaacaaa
    10261 tgtacttgat ttggtgaata tcactgcaca aggatttgca tttgtggcga tcatcaattt
    10321 gccggaacat gtatcaagca gtgatgtatt aaaagcaatt gactggaaca acgcctactt
    10381 gtatattgcc ggtgccccca tcaaggttac tagcggaagt ttattatcag caggaagcca
    10441 taccttgcgt tttgggatgg ggtcgtggga tacaaatact ctattgacag atattattgc
    10501 tattattacg cgaggcgaat tcaacgtgga cgatatcccg cttcaatttg atgttacagc
    10561 taatgtcgcg aagatgacag aaaacggaga taaatatgat acgagtccaa acaaaatttt
    10621 gaccaagggt aaattacaac cttctatgtc taaaaaggcc gttttttctg ttgattttta
    10681 tgattcgaat aatattatcc aagatcgtat gttcaattcg attttagctc cagctgcagg
    10741 tagattttat cctaaattaa ctggcggaaa cttgaatcca ggagcatatg cattttctgc
    10801 aagtgcagag ataaatacgt ggaatagtta catctcacca agtgattcaa caggaaaata
    10861 taatacattt gctgatacca cagaatacgt ggatggaaat aaccagaacc ttccaggctc
    10921 taccaatagt cggacattga cgatggggct gactgaagga cgttttgatc agttgccatt
    10981 tagtcgcttt gatcgcgtag tcaactattt tacaggaaaa aacgttacac aaggagcaat
    11041 actgactcat actccagcaa gcacaagtgg attgaatcag acagccaata tcgtgtacag
    11101 tgggaaagat gcgcaaggcg gattgctttc tcctgttgct ttaaaggtat tggagcagta
    11161 caagctagac ggaacggttt taccaacaaa cttaacagcc gatagtcagt ggggaaagct
    11221 cctttcttct gcatcgtacc aagtcacggc caattggtca gcaactgaac taaaaaaggg
    11281 ttcgatccat tacaggcttt attcgaaagg aacccaaaaa ttagcgacag gatggacaga
    11341 tcaacttttc caaacgatcc aatcaaataa cgggggtgta aatacagcta cttctacact
    11401 acctgcattg gcttcagggc aatattattt tgattatcgc ttggtggacg atgtacttac
    11461 taacatgtat ccgtcttttg cgtacaaatg gcagtctgaa caagctggaa tcaccactct
    11521 tcctcaaata acagtggcta attttccatc tgtgagtgca gatagtcagc taaaaaacct
    11581 tagcagacaa cctgagacag gagatcccct gtctgctcta tcaggagata cgattcaaga
    11641 aaatgtgaca ttcacgttaa caaaatctgg ggatgcgata accgacaaaa aaatcacgat
    11701 ctcacttcct gaaaatacta cttatatcaa tggttcctta aaattaaatg gtacaactat
    11761 cgcagatacc ggcattcaac aaggagtttc tgtcccagca gatctattga gcaaaatagg
    11821 agataccatt catcttactt acaactatca attaaatact gtagatactt ctttacaatc
    11881 cgtcagcata ctgacaaagg cagctgtact ttccagcaat attacattag cagatggtgc
    11941 taagcttcct aacccagttg tccaaacatc tgctaaaaca attcttgtcc ccaaacaaga
    12001 attaacatta gttaatgtac cagatgattt tacatttggt aacgatctcc ccaaaccgtt
    12061 gaagacaagt tattatgaat caaaaggcga tttttctttt gatgtgcggg atactcggct
    12121 tccaagcacg tctccttggc aactgacagg tacattgact tctttattta agaatgatca
    12181 aggtcaagaa ttaagcggaa caaaattata cttcaatcat tctggatcaa agcaattgat
    12241 tcagcaaggt caaaacacgt tgatttatga gagtgatgga acagctaaag gagaggttct
    12301 cgttgacttt cccgataccg atggtttact cttagaggta aatagcagta cgaatgctca
    12361 acctggcgca acgtatcaag gcatggtcac atgggaactg acagctgggc caaccagcta
    12421 agtgattaga atttgtatgg atctgtgata actgtcacag atcctctttt tcaaggatga
    12481 gaaggaaact tctgctgtta aaatcagaaa tgcttgaagg agtggacttg gaacttttca
    12541 gtttgcttga aaaaaatgaa caacggcaaa tcaaactttt ccaactttta ttagaggaaa
    12601 aaggagaggt ttccgttaat aacgtactta cccgcttatc agttgatcgg gctaccttga
    12661 aagaagatct gttttattta caagctaact tggaaaatat caaacaattg gaaattgtta
    12721 tccaagaagg gaatatttta ttaatcagac atggaaatat agcagcagtc gatgtttatt
    12781 atcattgtta cttgcgctat tctacaaaat atcagatact tctttatctg ttggaaaatg
    12841 gctcatttga tcggcagaaa cttgtcatgg aacttaatat cagttcggct actttgtctc
    12901 gcagagtgaa ggaattaaat gattctttaa atgagtttca tctgaaaata aaaagcgggc
    12961 ggctgaccgg tccagaaagt caaattcgtt atttttattt ccaattattt tggtttggac
    13021 gtccctatct tgaaaacaaa aaagaataca gtgatttatc aggaaacact tttttagatc
    13081 tgctgcaaaa agagtttagt tttgctatta cagaagaagg acgattgaaa tttgaaatat
    13141 ggctttccat tatgaagaag cgaatgcgct gtacgaaaaa atccaaacca gattatagca
    13201 atcttcctgt tggattgttt caaacgacac aatttttatt ggacctccat acatttttga
    13261 cccgttactt ctttcaatat gcctttcctt ggaatcaaaa tgaaacgttg gttttttatc
    13321 ttttcatgat cagtaacttt acattagata cacataactc gcatgtccag gagtttttga
    13381 taagcgaaaa atacaaagac gacgtagtag ctcaattgaa tcgaacgttt aaacaatcga
    13441 tagaagaata tttctctttt tatgtttttc cagaagagat ttcgcaaaag attgcgttga
    13501 cagtgatgca aaatcacgct cattttgcct attttagagg atggatgaat atttttggcc
    13561 aagaaggatt aaaacagcgg ttaactgaag tagaagataa acaattcctg gttttatgtg
    13621 aaagattggt cagacaaaca tttgctattg ttgattttac tgaagtagac gaggaaaatg
    13681 cccgctttga attatttgct cgctatgcat ctattttacg gacgctgatc aagcagttag
    13741 aattacgtct gcaaattgct tatgatcttc cttatgaaag gctaatggcg gatatcgtga
    13801 aagaaatcat ccaggaacaa ttgctgaata cgatcaaata tgatttagtc gaatacgaga
    13861 aagaaaagca gtatgatatc attttgacta gccaattgaa agagtacccg tcacagaagt
    13921 ccgctaaagt ttttgtattc actagcaacg aatataatta tgatttcccc tatttgaacc
    13981 aatttttaaa agaatgttat ttagaaaaat tgaatttaag gatgaataaa acatgaaaat
    14041 cgtgatcatt ggtgcttccc atgaaggcat cacagctgcc ctaaatctaa gaaaacttca
    14101 accgaaagca gaagttttac tgatcgatga agatcataaa gatggactag gctatgtttc
    14161 aaatggaatc aacctttatt taaaaggaaa gatacgtagt ttagctgaag ttgcccataa
    14221 catgcggact cttcaagaaa gtggggcaaa actgatcacc gaatggagag taactgaact
    14281 tgatccagac aaacatcagc tcatcctcgc atccaaagaa ggaaaaaacg agaccataac
    14341 ctatgataaa ttaatcgtag cgacaggttc ctctcctgtc accttgtata aacaaattga
    14401 agctgaaaat gtctacacct ataaaaatct agtgcaatct aagcaagtac ttgccgcttt
    14461 aaaagaagca aaagaagtag tgatcttcgg tgctggttac attggattgg aattagcaga
    14521 tgctttgaga aataaaggat acattattca tctagttgat tatatgccga atgttttatc
    14581 aagatatttc gataaggata tgatcaacag ttttcaaaat cagctgcaaa ctaagcaaat
    14641 aaatttttat ccaaatgaat tcttgatcga ttggaaaaaa tcagaagaga aagtagtatc
    14701 cgtacaactt ctgtctcaaa caataaaggc agatatggtc attttttctg cccaaactcg
    14761 tcctaatacg acattgttaa aagaaaaagt ggctttgtat gaggatgaga ctgtgatagt
    14821 caatgaatat ctgcagacaa gtgatccgga catctatgcg attggagata tcgtcccagt
    14881 cagttttgac aaaaataaac ggcatctctt tttaccttta gttactcgag ctgtacatat
    14941 ggcaagagca gtagcgctaa ctttgagtgg tcagccaacc gcttacgacc tcagacaaaa
    15001 gataacagca gcagtcatta tagactactt tttaggaact gtagggctga cagaagatga
    15061 agcgccattc ttggaacaaa ataccggttc gtgttcagga gaatttgatc tatttccaca
    15121 atacgacgaa gaaaacaaaa cagtaaatgc aaagctgatc taccatccgg atacattaga
    15181 aataattggc ggacagctga taagccaaga attcttatta tcagatttga acctgcttgc
    15241 ggatatcgtg aaacacaaaa ctacgattcc acagctagca gttgaggatt ttggatttct
    15301 gtctgaatac acctctcgtt ttcactacct caatgagtta gcttttaaag tactagctaa
    15361 gagagcaaat agcaatagag tagataaaag actgtgagaa atatcacggt cttttattgt
    15421 gtggggatat aataaatatt aatcaatcga gaataaaaaa ttcatataat gaaatagcgg
    15481 aagaattttt tgagcataat attcgccaaa aatttatcaa gtgataaaat tgttgtataa
    15541 aatgattatt tcaggaggag gagttaaatg tttttagcat taaatgaaat tatgcattca
    15601 aagttacgtt atgcgttagt tgcaggggtg atgtttttga tcgcctacct ggtatttttt
    15661 ttgactgggt tagcttatgg cttagctcaa gataatcgaa cagcagtcga caagtgggag
    15721 gcagattcga ttgtgctttc gaaagatgca aattcaaatc tagggatgtc catgattact
    15781 aagaagatag cagaagaagt cgaaggtggg aaagtggcgt atcttgccca aacacctgga
    15841 gtcgtgacta gtaaagacag tacagaagaa ggaaaaatca atgttagctt cttcggtatc
    15901 gataaaaatc aattcatcat gcccaatctt gttgaaggaa aagcttttga taacgatgat
    15961 gaggcagtag gcgatattag tttgaaagaa gaatatggtc tagccatagg agatacagtt
    16021 aagttatctg gtagtgacaa gacgtttaaa ctaactggct ttacggatca tgccaaattc
    16081 aatgtctcac cagttctcta tacaaccatc aatgcttacc aagaaatccg cttcgaaaaa
    16141 gaagatacaa gtgaaaatgc acggattaac gcaatagttg tcagaggtaa aatcagtaat
    16201 ttacctgaag atttagaaca gatcaaaatt tctaaattca tcaatgaatt acctggatat
    16261 aatgcgcaag tattgacttt tggatttatg atcggatttt tgatcgttat tgcggctatt
    16321 gtgatcggga ttttcattta tgtactgacg atgcaaaaga ttaatatctt tggtgtaatg
    16381 aaagcacaag ggatcacagg aggatttatt gcgcgttctg tagtggcaca aacattcatt
    16441 ttatcatttg ttggtattct tcttggtcta ttaggaacag tgggaacatc ccttgttcta
    16501 cctgatgcgg ttccattcca aagcaattgg ctgttttttg gcgtcattag cttgttgatg
    16561 ttagtagtag ctgtgttagg cgcactattt tctgttcgga caatcgtaaa aatcgatccg
    16621 ttgaaggcaa ttggataaga aggaggaaga acatgaaagc aatagaattt aaagaagtag
    16681 aaaaaagttt ccaagatggc gaccaagtag ttaaagcatt gaaagaaaca accatgagta
    16741 tcgataaagg ggaatttgtt gcaatcatcg gtccttctgg ttcaggtaaa agtacttttt
    16801 tgactttggc aggcggattg cagacgccta gcagtggctc tgtatttatc aatgatcaag
    16861 aattcagtaa tcaaaaagaa agcgaacgcg ttaaactacg ttttaaagaa attggcttta
    16921 ttttgcaagc ttccaatctg gttccttttc tgacagtaaa aaagcagtta ttattagtag
    16981 ataagattaa acatgaacat cgtcaaaaag aagcaaacga attatttgag cagcttggcg
    17041 tggataagtt gatcaacaag tatccagaag aactatctgg tggagaacgg caaagggtcg
    17101 caatcgctcg tgcattgtac aatgatccgt ccattattct agcagatgaa ccaactgcta
    17161 gtttggattc agaaaaggca cgagaagtag tcgatatctt ggcaaaagaa tcaaaagaca
    17221 aaaataaagc aacgatcatg gttactcacg atacacggct gattgacaaa tgcgatagaa
    17281 tatttatgat ggaagatggt gtattgaaag aaaaagaata accaaaaagc aggtggaaaa
    17341 atggtccatc gatacaacaa agatacacag caaaaaattt tgaaagaagc gactgacttg
    17401 tttatgtcca agggatatct cggcacttca acaagagaaa tcgcacaaaa agttgggatc
    17461 acccagccca atctctatca ttatttcggt gataaagaaa aactatatac ggcagtttta
    17521 gaaaaccact taaaagatgt tggaaaagct ttgagagaaa tcgttcaaac aagcgaaacg
    17581 ggatttcaag taaccttgac aaaaatggcg cagtttttga ttgagacaca tctggtcgat
    17641 ttgtttctga tgcttcacga tcttgaaagc aatctttcaa aggaaacaag agatcatctt
    17701 ttctttctat ggaagaaaaa ttatcgtgaa ccttttgaag aaatcttttc agcaaaccaa
    17761 tctgtgctaa gaaatgggat ttcaaaagaa atcgcagcaa ggcatttttt cttagtgtta
    17821 gcaccttaca tcactcggtc gaaaagacct gtggaagaac cgctaaatgt ggcgcaattg
    17881 atcgatttat atttgaatgg tgtattagga gaagcgtaaa gctgttatat agggagaaaa
    17941 tagttaggtg aagaagcaag atatgacgaa gatcgttata tcttgcttct ttatataggt
    18001 agtttttcat aaatacaaat ttgttattct ctcatgatta tcagggtata tttttattta
    18061 ttatgtcggt gttccaggaa gtgttttaat ttaataacga agaaattaat tgtttgtaga
    18121 atgaaggaaa ctatttattg ggtagatgaa tcaccacgaa tagtcaaaag gagatttcag
    18181 gatatgccaa aaatgaaaaa aatcatttta gcagtaatgt tattttctac ggttatatct
    18241 tttacagccc cttttttgct tacttaagct gattttaagt tttggccctt agtctggatc
    18301 tacgaggtga gagtcaagaa tgaaaaaaat aattggagct tttgtatttg caatcattat
    18361 cattattatc tgttttcaaa tagatgtttt tggtcaaaaa aagatagttg gacaaacggt
    18421 cagcagtacg aacagtacgg ttacaaagac aaaggaatca tttgatgata cagaacaaga
    18481 gacattaatc aaaatcggaa aaaatgaccg tacactatat acagatgact cgctaactac
    18541 cccacttgca actataaacg gcggagagct ggcggagttt ctttctgaaa caaaaaatgc
    18601 ttatcagatc aagacaaatg atggctatac tggatattta gccctcgaag atggaaccaa
    18661 agtcacaaag aatatacaaa caaaaccgga agagctatct gatgcagtga ttgtattaga
    18721 tcctgggcat ggcggaaatg atactggggc attaagcaat gatgaacaaa cagaagaaaa
    18781 agatataaca ttaagtacag ccataaaagt aaaaaaggcg ctagaggatg ctggagcaac
    18841 ggtttatttg acccatacta cagatgaatt agtacagctt ggagatatat gcgactacag
    18901 tgaagaaaaa aaggcagatg tctttatcag tttacacgca gactctacgg aatatgcgaa
    18961 tgaagcaact ggaatcacga catattatta ctatggacaa gaggaaacat tagcacagac
    19021 aattgctgac tccttcactg atctgcctct ggattcaaga ggaatctcca ctgggaacta
    19081 tcaagttctt cgagaaaatc ttcaaccttc tattttaatc gagatgggtt atatgaataa
    19141 tgatagtgat ttaaaggaat tagtaacaga cagttatcag cagcagattg ctgcaagttt
    19201 gacacaagca ttgaccactt attttcagta ataaataagc ccagccaaac aaaaatagct
    19261 cctcatattt ttcgtttggc cgggcttatt atcgcagatg caagctttta aacaccgttt
    19321 aaggaagagg tcgtgagcct gccgttcaac tctgagtcac aaggagcaat ttcaaaaaac
    19381 agcttctcat gtttgatgac tattgtgact tgtgaccgag gagttggcat gagaacaccg
    19441 tttaagaaaa aggttgtgaa atcaacgttt tgttctgagt attagagaaa caaacggaaa
    19501 aatagttcct catattttgc cgtttgttga gctaataccg aggatcagat gattgaacac
    19561 cgtttattcg gaacaaggga ctgtgacatg acttttgtca cagttccatt atttttttag
    19621 tagcttcaaa gctttttctg tattggcaaa atgaagtttg gcatattttt caagcatagg
    19681 catgccgcct ttctccaata gttttgccgc tacttggtca gatttcgttc cagctcctga
    19741 cggaagggtg attccagctt cagctgatgc tttgtctaac tcttccaaaa agctggcacg
    19801 actgatgata gaagcagcag caacagctaa atgatattgt tctcctttag taacgaagta
    19861 cagtttttct tgtaattgat ttttttctaa acgcgcatat tttttaaaat ttgcttccgg
    19921 cgtaaactga tcgatcaaaa tcgcttctgg tttttctggc tcgatttttt ttagcagcaa
    19981 atggatcgct tgattgtgca aagcgacttt catccgaacg gcattgtatt ttggttggat
    20041 ctcattgtat ttttctggag ataagacaag cagtttatag gggatggttt tttttaagat
    20101 gtcagcaagc tggatgattt ggctatcttt taactctttt gaatctctaa caccaagttt
    20161 acgtaattct gcaagctgat cacgtttcac ataagcagca catactgtta gcggaccgaa
    20221 atagctgcca tttccgacct catcactacc taaaacggat aattgactga tattttttgg
    20281 caaagaagag gctgtttttg ctggcttggc ttgtttatcc tgtgcctccc cccatttatc
    20341 agcttctatt gctgcattcg taccttgaaa catcactttt ccggatgtat aagcggtaat
    20401 ggtcgtcccg tctttttttg cagcaaagct agtataagga atctgtttat ttaacaaata
    20461 agagcgatag tatgctttca tttgactaag ttggtcgttt gttgctttga taatgacgtt
    20521 gctcataaaa ttctcctcgt ttttatacat aaaactatac cgcaactcac gacttttcgt
    20581 tttctaagag attgcgagac atagcatttt tttaaagttt atcatataat agttaagtat
    20641 gtgaaaaata tttgtggaga acggtgaaag aaccaacttc atggtacact aaataaaact
    20701 ttcatcaaat aggaggaaat gattcgtatg gcgcacgaaa aaacacgcta caaagcggtg
    20761 attgccaacc aaacatatac tattatcgga cgggaaacca agcatcatat ggatatcgta
    20821 acgaagctga tcaatgaaca gttagcagaa ttgaaacaat tatcaccaca aatggataat
    20881 gaacaagcag caatcttgat ggctgtcaat gcgctttcgg atcaattgaa gaaacaagaa
    20941 agaatattgg agctggaaga agaaacagca gaattaaaga aaaaaatgat caaatttaca
    21001 gagcttgaaa atcgtgtgaa acgcattgaa gcaatcgaga acgaagctcg ggaagtattg
    21061 aaagaaaatg gtcaagcaga tcataccatt aaaaatcatg tggaagcaca acagatttta
    21121 aacgagaaac gaaaaggaca aattaaacaa aaatcttcta attaagatcc ttttatcaaa
    21181 ataagaaaaa tttaaaaaag ttagaagaga aaggaacgta gcatgctgag tttgctgatt
    21241 ttatttttat tgctaattgc gtttttttct ggagcacgtc gcgggtttgc tttgcaaatc
    21301 atattcgcga ttggatatgt attgtcattt attgctgccc agcattttta caaaccatta
    21361 gcaagtcatt tggaattata cattccatac ccagctgtca ccccaacatc aaagttagca
    21421 ttttttgatc aggtatttgc ctttcaccta gatgaagctt tttatgcagg aactgctttt
    21481 ctgattattt tgctaattgg ctggctgctt acccgatttg tgggggtttt tgtccatgga
    21541 ttgacgtatg tgccgatctt gagacaggta gactggattg caggaggcat cctgtcacta
    21601 atcatggctt acgttactat atttttgatt ttgcagttac ttgcctttgt tccattagat
    21661 gtggtccaaa atcaattcgg cgagcatagt ttcgctcgtt ttatcgttga gagaacgcca
    21721 tttcttacca acaagatgta tgagctttgg gtaacgaata ttttgaatta aggaaaatag
    21781 tgctggaaaa tatttcgttt tttgtacaga gtagtttatt taagaaggct gcgtagcgta
    21841 tgtccggcac agctcttttt tcatagtgaa agataaggtg ataaaatgaa taaccgaatc
    21901 cttgaaacat tggaatttga aaaagtcaaa cagatggtca gacaatttgt agtgactgca
    21961 caaggaaaag aagaactggc agaattggta ccagtgagtg agaaacaaac gatcacaaac
    22021 tggcttcaag aaactgagga tggattgaaa gtgcaacgtc tgcgaggcgg gatccctata
    22081 cctaaaattg aaaatatccg tccccatatg aaacggattg agatcggagc agacttgaat
    22141 ggagtggagc ttgcacaagt ttccagagta ttgtctacga caagcgaatt gaatcgtttc
    22201 attgatgatc tttctgacag tgaaatcgaa tttgcacgtt tgtatatgtg ggcagatcag
    22261 ttagtcacga tccctgtttt gagccgccgt ttaaaggaag caatcgatga agacggacgc
    22321 gtgactgatg acgcttctcc tgagttaaag agcataagac agaacattcg gcgaagtgaa
    22381 caagctgttc gtgaacagtt agatgggatc gtccgtggga aaaatgcaaa gtatttaagt
    22441 gatgcgatca tcaccatgcg aaatgaccgc tacgttatcc cagtcaaaca agaataccgc
    22501 ggtgtatttg gcggtgtagt ccacgatcaa agtgcttcag gtcagacttt atttattgaa
    22561 ccaaaacaag tggtggattt gaataatcgt ttgcgtcaat accaaatcgc tgaacgaaat
    22621 gagattcaac gtattttaag tgaactttct gctgaacttg ttccccatcg tcaagaaatc
    22681 atccacaata cttatgtgat tggtaaaatg gatctgatga atgcaaaagc acgatttggt
    22741 aaagaagtaa aagccatcgt gccgggaatc agtgaagaca atcatgtcgt cttgaaacaa
    22801 gcaagacatc cactgatcga tcaggaaaaa gtagtttcta atgacatcac aatcggaaaa
    22861 gactatcaag ccatcgtcat tacaggacct aacacaggtg ggaaaacgat cacgttgaag
    22921 actttaggtc tgttacaact gatggggcaa gcaggattac cgattcctgc aggtgaagaa
    22981 agtcaaatcg gtatttttga ggaagtattt gcggatatag gagacgaaca gtcgattgag
    23041 caaagtctat cgaccttttc ttctcatatg acgaacactg tagatatttt atcaaaagtc
    23101 aatgaaaaaa gtctggtatt atttgacgag ctcggtgcag gaacagatcc gcaagaaggt
    23161 gctgctctag caatcgcaat cttagatgat cttggtaaaa agtctgctta tgtgatggca
    23221 acaacgcatt atcctgaatt gaaagtttat ggatataatc gggcaaatac gattaatgcc
    23281 agcatggagt ttgatgtgga tacattaagt ccaacgtatc gtttgctgat tggcgttcct
    23341 ggacgaagca atgcgtttga aatctctagc cgattaggtc ttgatacaga agttattgat
    23401 gaagcgaagc agttgatgaa tgatgagagc caagatttaa acgaaatgat cacagattta
    23461 gagaatcgcc ggaaaatggc agagaccgaa tatctggaaa tgcgtcattt tgtttctgag
    23521 gcacaagaac ttcacgatga tttgaaagaa gcttacagct atttctttga agaacgtgaa
    23581 aaagaaatgg aaaaagcaaa gaaaaaagca aatgaagtcg tgtcagaggc ggaagaaaaa
    23641 gcagaaaaaa tcattgctga tattcgtaaa atgcaacagc agattgggca aggaaatgtg
    23701 aaagagcatc agttgatcga tgcaaagacc caattagcaa atctacatca agaagaaact
    23761 ttgaagaaaa ataaagtatt gaagaaggcg aaagaacaaa aaacgttgaa accgggagac
    23821 gaagtgttgg ttacgactta cggccaaaga gggacgcttt tacgtaaaaa cgggaatcag
    23881 tggcaagtag aaatcggtat attaaaaatg aatgtgtccg aagacgagct gacgcctgtt
    23941 gctcctcaaa aagagccgac acaacgagtt atccatgctg tacgatctga atctagtagt
    24001 cacgtgccga atcaattgga ccttcgagga aaacgatacg aagaagcgct aagtgaggta
    24061 gatcaatatt tggattctgc cattctagct ggttatcctc aagtgactat tgtccacgga
    24121 aaaggaacag gcgcacttcg caagggaatt actgattatc ttaaaaacca tcggagcgtc
    24181 aaaagctttg agtttgcacc ggcaaaccaa ggcggaaatg gtgcgacaat tgttaaattt
    24241 aagtaaagct tactaaaggt aagcagatgg ttcgctatcc aagtatagaa atgctatact
    24301 agtcatagat tttaaaaatg gaggtctttg atatgtcaca agtaattaca gataagacat
    24361 ttaatgaaga aacagataaa ggacttgtat tgattgattt ttgggcaact tggtgtggtc
    24421 catgtcgtat gcaagcaccg attttggatc agttggaaca agaatatgat gaagaagaat
    24481 tcagaattgc taaaatggat gtggatgaaa atccagaaac tcctcaacaa ttcggtatca
    24541 tgagcattcc aacattaatg ttgaaaaaag atggtcaagt agtagaaaaa gctgttggtg
    24601 ttcattcaaa agaacaacta cgtcaaatga tcgatcaata tttataaaaa cttacagcaa
    24661 aaataagctg agggtgggac tgaatccagt tccgtcctcg gtttttttgc ggttgagtta
    24721 aggttattca aattaatatt ttttgagtat aattacgttg tcaggctgga ttgttttttt
    24781 gacagacatg ctataataac tttaattgaa tatgaagggg agtaccagat gaacgaacga
    24841 atcaaaaaca aattagcttt acttcctgat cagcccggct gttacttgat gaaagataaa
    24901 aatggaacga tcatttatgt cggcaaagcc aaaatattaa aaaatcgtgt ccgctcatat
    24961 tttactggta gccataatac gaaaacggaa cggcttgtta gtgaaatcga agactttgaa
    25021 tacatcgtta cagaatcgaa tatcgaagca ttgctcttag agatcaatct gataaagaaa
    25081 aatgatccta aatacaacat tatgttaaag gatgataaaa cctatccttt ccttaagata
    25141 acgaatgaaa aatatccccg tttggtgatt actcgtaagg tactaaaaga caaagcactg
    25201 tattttggac catatcccga tgtcggtgcg gcgaatgaga caaagaagat cctcgatcga
    25261 ttatttcctc tgagaaaatg caaacctagt cagacgaagg agccttgtct ttactatcat
    25321 ttgggacaat gcctgtgtcc ttattatttc gacgtagatc cgaaagtcta tacgaaaatc
    25381 gttgatgaag tgaaaaactt tttaaacgga ggacacgaaa aaatcgagtc agagatccat
    25441 cagaaaatgg aaaaagctgc tgaaaacatg gagtttgaaa aagctgcaga ataccgagac
    25501 cagatccgtg ccattgagac aatcatgact cgccaaaaaa tgacaaatac tgatttactg
    25561 gatcgagatg tatttggtta tgcgattgat aaaggatgga tgtgtgtcca agtgtttttc
    25621 gtaagacaag gaaagctaat tgaacgagat gtttcaatgt ttcctttcta taacgaagca
    25681 gaagaagatt tcttgactta cctcggtcag ttttatcagg aaaatgagca tttcattcct
    25741 aaagaagtct tgattcctga tacgatcgac aagcccagtg tagaagcttt gttagctacg
    25801 aaagtgctgc aaccaaaacg aggagaaaag aaaaaattag ttgaattggc gagtaagaat
    25861 gcctcagttg ctttgaatga acggtttgat ctgattgcca gaaaacagga acggacgatc
    25921 ggagcagttg aacgtttagg aaacgcgatg aatatcccga ccccaatacg tatcgagtct
    25981 tttgataact ctaatatcat ggggacgaat cctgtggctg cgatggtcgt attcattgat
    26041 ggaaaaccgg caagaaaaga atatcggaaa tataaaataa aaacagtcaa cggaccggat
    26101 gattatgcat caatgagaga agtcatctat cgcagatatt cgcgagtgat tcgcgaaggg
    26161 ttgcctttac ctgatttgat cttgatcgat ggaggaaaag gacaagtaga tgtggcaaaa
    26221 gatgtcttag aaaatcaatt aggaatcgat attcctattg ctggcatggt aaaaaacgat
    26281 aaacataaaa caaatgagtt gctattcggt acagatttgg ctgtagtacc gcttgagcga
    26341 aattcaccag agtttttctt actccagcga atccaagatg aagtccatcg attcgcaatc
    26401 actttccatc ggcagctacg cagtaaaaac agttttgctt cccgattaga tgaaattgaa
    26461 gggttaggac caaaacgaaa aaaaatgctg cttaaagagt tcaaatcgtt gaaaaatatc
    26521 acagcagcca cgacagaaga attacaagaa atcggactgc cgaaaaatgt cgcacaaaac
    26581 gtatttgata aattacatca ggaatgagga aagggcactt tacaaagaaa gtaaagtgtc
    26641 ctatcatttt atgtacaaca aaaagagcgt ttacgttttg ttggtaaata ataaatcgct
    26701 tacgtctggt gtctaaggat atatgtgatt tcttgaagaa ggagtgaaga aatgttagaa
    26761 gtcaaggatc ttgttaaaac ttttgggaac tacacagctg tcgatcatgt ctctttccaa
    26821 atccccgatg gaaaaatcat ggggctaatt ggccaaaacg gggctgggaa gacaacgact
    26881 tttcgtttga tcttggattt tttgacacaa gaccaaggag aagtgttatg gaatggccat
    26941 catcttgggg agaaagatta tgatgtcatc ggttatcttc cggaggaacg gggactttat
    27001 ccgaaagtaa cgattcaaga ccaactgctt tattttgcag aacttagagg gaaatcaaga
    27061 aaagagattg aaccaaaaat cgacttttgg atggaaaaat ttcaggtaaa agggaaaaag
    27121 acggataaag taaaatcttt gtctaaagga aatcagcaaa aagtccagct gatcgccacg
    27181 ttaatccatg aacctaaatt gatcattttg gatgagccat tcagcgggtt agatccggta
    27241 aatgctgaat tgctaaaaga tggaatattg gaattgaaaa aacatggttc atgtgtcatt
    27301 ttttctagcc acaatatgga taatgtagaa aaaatctgcg atcatttgat catgttgcga
    27361 aacggaaaaa tggttttgaa tggaaaagta catgagattc gtgaatcatt tggccgtacg
    27421 aagttattct tggaatctgg tttaagccaa caggaagtag aagaaatcga aggcgtgaaa
    27481 aaagtgatcc ttcatgaaga caatacgtta gagattacct tagaagatcc tgaagctggg
    27541 aaagaaatct ttactcgtgc gactcagttc ggctacattc ctatgtttaa ccagcaaccg
    27601 ccaactttgg aagagatttt caaattgaaa gcaggtgaaa tcgatgcgta agttttggat
    27661 tattaccaaa gatgtctatt taaaaaatgt taagtcgatt tctttcttga tcatgatatt
    27721 agtccctttt atcatgctgg gtatcattta tgtagcagga aactttgctc aacaaaatag
    27781 tgatgtcaat aaaatcggcg tattggctga taatgagcaa atcagtcagc aaatgagcca
    27841 agtcaaaaca gatgattttg agttcaaagc agttgattct gaagaaaaag cacaaaaaga
    27901 gctttcggaa gaaaaaatcg atgcgtattt agtagtctca actgagaatg ataagataaa
    27961 tgggatgctt tatagcgaaa attcattagg acaaagtaca caactactga tacaacaaca
    28021 actgactggc ttgcaatcga tgcttcgagc gagtcaatta ggcatttcac cagaagaagt
    28081 agctagcttg agccaacaag caagtttgac tcgacaaaaa gtcaattttg attcaaaagg
    28141 aaaaatgaca gtcggagaag ataactcgac agtccaatac gtcgtaagtt atgtagcaac
    28201 gattgtcttg ttcattatta ttttgacgta tgcgcagatc attgcacaag agatcgcttc
    28261 tgaaaaaggc acgcggatca tggaagtgat cctgtccagt acgactgcac aaaagcattt
    28321 ttacggaaaa ttaacaggtg ttttacttgt tgcattaacg caaatggcat tatacggtat
    28381 tatttttggt ataggataca gtcaattcaa agatatggaa atggtcaaaa atatattaga
    28441 cggtatctca ttggataact tgattggacc attcttgtgg tattctcttc tattcatgat
    28501 ctttggcatt ttgatttttt ctgttttagc agctttatgt ggctctttgg tcaacaaagc
    28561 agaagataca gcaaaagcaa ttttgccagt gacttatcta tctctaggcg gatacatgct
    28621 tggattgata ctaggagctt cagatccaaa caatatcgtt attcggatca cttcgtatat
    28681 tccattctta tcttcatata ttatgccgat tcgcttagca aatgagacag tcggtatggg
    28741 tggagcattg atttccttgt tggtacttgt agccgtaaca ttcttattga tgatcttgtc
    28801 tgcaaacatg tacaaatcga atgtactcgt ttatagcgaa ggcggattat ggagttcatt
    28861 gaaacaatcg atttcaatta tgaggaatga acgaaaaaaa gcataaaaca atcctaaaat
    28921 acagaaggaa gctggacatc tattgttccc agcttctttt tgtatagacc tattttattt
    28981 attttactat attggatata tattcattga aaatagtgta taacaacttt ataatattga
    29041 gtataccaat ttttggaggc gttttaatgg accgttattt agatttactt tcagaaaaat
    29101 tcccctcaac ggaggatgtc atcacggaaa tcatcaatct cgaagcaatt ttgcagcttc
    29161 caaaaggaac agagttattt ttgagtgata tacatggcga atttcctgct tttgatcata
    29221 ttctgcgtat cggttcagga aatcttaaag aaaagatcaa agaccttttt tctgaacaaa
    29281 tgacggaaac ggatatcagc caattcacta tttttacggc ttatccagaa tatgcattaa
    29341 cgactgattg gtacaaagaa caagataaat cgcagctgat ccatcatttg atcgatcttt
    29401 tacggtttac gacagtcaaa tatacccggt caaaagttcg aaaaggattg ccgaaagaat
    29461 atagctatat cattgaggaa ctcttgtata tcgatgaccg tatcaatgga aaaaaagact
    29521 atgtaaaaaa aatcattgaa caacttgttt tgatgaagga agaagaaaaa tttttaacga
    29581 aacttgcaca gacgatccaa aaatctgtca tcgatcattt gcatatcgtt ggagatattt
    29641 ttgaccgagg aactcaagca gccaaagtga tggatcgaat catggatttt tcttctgtag
    29701 atatccaatg gggaaatcat gatattttgt ggattggtgc gtattgcggc tctgaggcat
    29761 gtttactgac acttttacgg attgcagcac gctataatta tttatttgaa ttagagaaag
    29821 aatatgggtt gaatttacgt ccattgtttt cttttgctca tcaaacttat cagaagaatc
    29881 cagtatttgc gcctaaaaac agtaaaaatc tatccgaaag agagcaagaa gaattagaaa
    29941 aaatccatca ggcactttct atccttcaat tcaaatcaga acatcagtta ttggatcgtc
    30001 gacctgaatt taaaatggac gatcgtaaat tgctggaaaa aatcgactat gaagcatcaa
    30061 cgattgattt agaaggtcag ctctatgagt tgaaaggaac ttgttttcaa acgatccagc
    30121 cagatgatcc aaaaaaattc ttggaagagg aacaaaaagt gattgatagt ttgatgtatt
    30181 cattccagca ctctttgaga atgcaaaaac atatgacgtt tttgatcgaa aaagggggaa
    30241 tgtatctcac gaacaatcag catgtgctgt ttcatggctg tatacctgta gacgagaagg
    30301 gacagctctt agcatttgaa ttagaccaat cttatcgcgg aaaagaactg ctgggctttt
    30361 ttgaaaaaca gataactgaa agcgcaaaag atctgactgc aaaagaagat ttagcaaccg
    30421 atttgatttg gtatgcgtgg agtgggccat tttcgccttt attcggcaag agtaaaatgg
    30481 cgacttttga acgttatttt ctcacagagc ctcatacgca cgtcgagaaa agcaatcctt
    30541 attatcactt gcgagatgaa gagtggttca gtgaaaaaat tttagcagaa ttcgacacaa
    30601 aagaagaagg gtcagcaatc attaatgggc acacgccagt aaaagtaaaa aaaggggagt
    30661 caccgatcaa agcaaatgga aaagtatttg tcattgacgg aggtttatcc aaagcttatc
    30721 aaaaaacgac aggaattgcc ggttattcac tattatgcaa ttcttatggt ttccaaatcg
    30781 tgacacatta tccgtttttg cctattgaac aacaatttgc aaaaaaatcc gaccaaacaa
    30841 atgtgaaaaa agtaatcgaa cagcaactac cacgcaaact caatcgtgat acaacaaaag
    30901 gtatggaact gcagcagcag attacccagc tcaaaaggct gttggactat cgaaaggacg
    30961 attgagaaga attccagtga acattgttcc atagactgga gactgagaca aaagtcgtgt
    31021 cgcagtctct ttttccggat aaacggtgtt cttctgccaa ctcctcggtc acaagtcaca
    31081 ctattcaaca aacatgaaaa gctgtttttt gaaattgctc cttgtgactc agagttgaac
    31141 ggcaggctca cgacctcttg gtataaacgg tgttcaaaag ccagtttctc ggtattaagc
    31201 caaatttatg caaaaatgtg aaaagccatt ttcacaaaat ttttcttaat actcgaaact
    31261 aaacagcttt ttcacaaccc cctcagaaag tctattaaaa ttcattcaca aagaaatcga
    31321 ataagcgctg ttcagatgga tgtacacggt gggtaagttc cggatgccat tgaacaccga
    31381 ggattttatg catatcatct ttgctttgaa ccgcttcaac gagtccatcg ttagaaaaag
    31441 cgatggcttt taataaaggt gaaagatctt tgactgcctg atgatggtaa gaattcacaa
    31501 aatatggatc ggggagaagg ttgcttaaca aactgtctga aacaatcttg acttcgtgag
    31561 tcgtaaattg cggggcagtg ggctgttgtt catgttttac tgaccacttt ggatataatg
    31621 aaagatcttg ataaagtgta ccttccaaaa cgacattcaa tagttggagt ccccggcaga
    31681 ctgcaaaaat tggttttctt tgtttcaatg cttccaagat cagtgcctgt tcaaattggt
    31741 ctcgttgaat atttgtttcc gttagctttg gatgaggatc ttccatgtaa agttgtggac
    31801 tgacatcttg accacctgca agaataagtt tgtcgatttg tccaatcagt tgaggagctg
    31861 aatctggaga catgactggc agaataagag gcaatccttg agcatctaaa actgcatcca
    31921 cgaatccttg cggtgtatag ctcacttgat ttccttggaa agtatctgtt gctcgaatca
    31981 gttgatttcc ggcaatgccg atgattggtt tcatttgtat tccccctaaa gtagacttat
    32041 tgtaacaagg atttaaaaaa aattaaacta tgtaagactt gcccctatac ttatctgttc
    32101 ttgctgtgtt actatctgta aagagaaatt tcgtgtgaaa ttcatgacga tagttgaaaa
    32161 tattgcttaa tttgttataa taaaaagtaa gcagaaataa aaaggttggg cgtaagcttc
    32221 aaggatctag ttcttcaagg ggtgagaagg acattttatt gaaggggcct gcctatacta
    32281 agttatcttt agaggtacat actttcaatg aaaaaagaaa gcatgtacct cttttttaaa
    32341 tcgaaaggaa gtgaagcaag tgttagaagc tttggaacaa atccgaaaag cagaagaaga
    32401 aaatgaaaag cgtgctcaaa cactgcggca gcagctgaag atctatgcaa cagaaaaaga
    32461 gcaggaattg gaaacgatcc aaacaaacta tcgacaacaa ctaaaacagt tggtggaaga
    32521 aaaagaacaa tttgaaatac aagaattaga aaaagaaaaa atgaaattga aagcagaagc
    32581 agacaaggtg caagcaactt tgaaagatca atatcaaaag catcagagtc aagcgatcgg
    32641 tgcgatcatt gaaagggtga aagaaactta tggccgtcac taagatggaa aaagtgaccc
    32701 tcatttcaga taaaagaaat caggagagga ttttacaagc agtacaagga atccaaaacg
    32761 ttgaaatccg ggatctgtat caaaatagaa caaacaacca gtgggtggag aagtattttc
    32821 cagataccac gattattgat aaagaagcaa aattaagcgc tttaggtaat cggttgaaag
    32881 acatccaaga agctatccaa ttcatacaac accacggcga tagccgtcaa aagaaactcc
    32941 atttgaaacg tcaagaactt tccttacaag atttggaagc caattattcc gaagaaaatt
    33001 tcataaataa attgaaagaa gtccttgatt taaaagaaaa gtgggaacaa ctaactgaag
    33061 aacgtgatcg tttagaagaa gaggaagaat ggcttcttca ttggcaacag ctggatattg
    33121 ctccaagtat gtatgcaagt aagacgacca tttttgagat ggggactgtg agtgcagcaa
    33181 attttgaaag tttcaaggaa gaactgagta agcttgatga cgtttatatg gaagaaatag
    33241 attatacgcc aaaacaagtt tatctttcgt atgttgcttt aaaaaaatca gttgctgtta
    33301 tagaagaaat tgctgggaaa tacgggttca ataaagaaac gtatccttat gaacaaaatc
    33361 ctaaagaaaa actgcaagag accaagcagc aattatccat tgttcacgag cagcaaaaga
    33421 agataacgac agcgttaggt cagtgcagtg gctatatcca agaatttgaa tgggtagaag
    33481 aagtcacact tgccgttgca gaacgagaaa aaataaaaga acggtttatc catgcgactt
    33541 atctgatcgt tttgcaaggt tgggtagatg cagaagaaaa acagaatttg ctgcatgtgt
    33601 taaacgaaac cgtatcagaa gaagatatat acgtcagttt cgaaacacca aactcagaag
    33661 agattcagca ggaagtccct actaaattga aaaaccatcc gcttgtcgaa ccttttgaat
    33721 tgctgactga aatgtacagt ctgccaaaat atgaagaagt agatccaaca ccttggatga
    33781 caccatttta tcttgtcttc ttcgggatga tggtcgcaga tgttggttat ggtttgctga
    33841 tgctcattgg gacaatacta gctcaaaaac tacttgttct accacgcggg atgaaacgtt
    33901 tcgtaaaatt ctttgagatt ctctcgatac catcgatcat ctggggattg atctacagtt
    33961 catttttcgg acagtcatta ccaaaagaac tgtttggcat ccgcttgcct ttcccaatcc
    34021 tatctacgac ggatgacgtc aatacgattt tgattttatc cgtgattttc ggtctgatac
    34081 agattttggt tggattattt gtggcagcaa aggaaaatat caggagaaaa gattacttga
    34141 gtgctgtcag tgatggattt gcctggcaag ggatcctaat tgggatcgtt attgcgttgg
    34201 ttggtgccat gttgataaaa aatacagcat ttgtttacct tggcgctggg ctatcgatca
    34261 ttgctgcctt atgtatcttg atcgttccga ttatccaaac gccgtctaaa gtcaaaggtg
    34321 ctgccaaagg tgcgtataat ctttacggac tgaccggcta tatcggtgat ttagtcagtt
    34381 atacacgttt gatggcattg ggtatttcag gcggaagtat tggagcggca ttcaatatgc
    34441 tagttgcttt catgccgcca gctgctcgat tcagtgtagg gatccttttg atcatcgctt
    34501 tacacgcact gaatatgttt ttgacactgc taagtgccta tgtccatggc gcacgattgc
    34561 agtatgtcga attttttggg aagttttata caggaggcgg acgtgccttt gatccgctga
    34621 aaacggcgga aaaatacgtc aacatcaatc acaagaaaaa aaacaataaa taattggagg
    34681 atttttatat aatgatggat tacttaatta gtcaaaatgg tggaatggta tttgcggtat
    34741 tagcgatggc aacagcgacg attttttcag ggatcggttc agctaaaggt gttggaatga
    34801 caggtgaggc agcagcagca ttgacaacaa gtcaacccga aaaattcggg caggctctga
    34861 ttctgcaatt gcttccaggc acgcaaggtt tgtatggatt cgttattgcc tttttgattt
    34921 ttatcaactt aggaaatgac atgtcagttg tacaaggact gaactttcta ggtgcttctc
    34981 tgccgattgc ttttacaggc ttgttttcag gtattgccca aggaaaagtc gcagcggcag
    35041 gtattcaaat tttagcgaaa aaacctgaac atgctacaaa aggaatcatt tttgctgcga
    35101 tggttgaaac atacgcgatt ttaggtttcg ttatttcctt cctattagtg ttgaatgcct
    35161 aagcataaag gaggaaaagc agatgaacgc cattgaaaaa atcatctctc agatgaacga
    35221 agcagcagag caggaaagag cggcgttaga acaagaagag cgaatgaaga tcgatcaaaa
    35281 ctttgaacaa aaacgtacgc aagttgaaac agaacatcaa aaacaaaaag aaaaacaaat
    35341 cgaattactc gaaaaaaaat accgtcagct aaggaatcgc cagcaagtag aagtacgaca
    35401 agaaaatttg aatgcgaagc aggaattttt aagacgattg tttgcagatg cggtaactga
    35461 aatggaaaat tgggatgaat ctgaacaaat ccagtttatc aaaaatgcat tgtattcatt
    35521 acctttgaca ggaaaagtag cattcattgc tggagaaaaa tcagcagcct atctttctca
    35581 gacgttgttg gatgaatgga acaatgaact tccctttatg atggttttaa gtgatgaaac
    35641 tgttgctgat caagcgggtt ttctgataaa cgatcaagga gttcagtata attttctttt
    35701 cagcagcctt gtacaagata tccaaggaac gatgagtttt gagattgcca atcaattatt
    35761 cgaataagga gggatcagtg tatggaatac cacgaactaa atcccttgat tcgcggaaga
    35821 gagcttgaac ttttgtccaa tgagacattt gaacggatga ttcaaacaaa ttctgtcgaa
    35881 gcacttggtg aaatattgaa atccacgatt tacagtccat atatttatga cggatatgaa
    35941 tatgacttcg aacagaatct tttaaaggag cagagccgtc tatttcgctg gttaaaggaa
    36001 tcagcaccgg agcctgaaat cgtttggatc tatacgatgc gttatacttt ccataatctg
    36061 aaggttttga caaaagctga gatgacaaat caaaatctgg accatttgta tatcgacgat
    36121 ggattttatt cattagaaac cttgaaggat gccattcata cgcaagtttc aaatgaactt
    36181 cctgaaagca ttatggacag tatacgagaa gtacgtgatt attttgaaga gtctaccatt
    36241 cttcagggaa ttgacgtgat ttatgatcga cagtttttga cagagcagcg acggcttgcc
    36301 gataagctag gatatcaaga attgctgaca gaagtcattg cttttattga cttgaccaat
    36361 atcacaacga tggctcgagg aatattgcag aaaagaacat atgggttcat gacgacggta
    36421 ctttccagct cgggaagtat cgaaaaagaa gtatttttga actttgtaga taaagatatg
    36481 gaagattaca cgaattttct attagagaca gattatgctg attttctgcg ccctgtcatc
    36541 cgtgagggaa atattgatct cgtaaagctt gaacagttga aagatgatta tctaagttca
    36601 ttgtatcaag aagcacaaac ccaggctttc ggaccactgc cactactagc ctttttgaac
    36661 gcaaaagagg tagaaagcaa aaaccttcgt ttactggtta tcggcaaaag aagccatttc
    36721 gaatcagaaa caatcagaaa gcgggtgaga caagtctatg acgcataaga tcggtgtagt
    36781 tggcgacaaa gattctgttt tgccattcaa attgtttggc tttgatgttc gttatggtac
    36841 aaccaagcaa accgttcgtc aggcaattga agaaatggca aagaatgagt atggcgtgat
    36901 ttatgtcaca gagcaatgtg cagcattgag cccagaaacg atcgaacgtt acaaggaaca
    36961 aatgatacct gctatcgtat tgattcccaa tcatcaagga tcattaggga tagggctgga
    37021 agaaattcaa aaaaacgtag aaaaagcagt aggtcaaaat atcttatagt atgaaaagga
    37081 gatagaaatt ttgcaaattg gaaaaatcat aaaagtctcc ggacctcttg taatggctga
    37141 gaacatgtct gatgccagta tccaagacat gtgtctagtg ggagatctag gagttatcgg
    37201 agaaattatc gagatgcgtg gcgatgtggc gtctattcaa gtatatgaag agacttctgg
    37261 aattggtcca ggcgaaccgg ttcgctctac tggtgaagct ttatctgttg aattaggacc
    37321 agggatcatt tcacaaatgt ttgacggaat ccaacggcca ttagatacct ttatggaaat
    37381 cacacaaagt aatttcttag ggcgcggagt tcaattgccc gcattggacc atgagaaaaa
    37441 atggtggttc gaaccaacag tagaagccgg agaaacggtg agtgctggag atgttatcgg
    37501 gatcgtagaa gaaacaaaag ttataaaaca taaaatcatg gtgccaaatg ggataaaagg
    37561 aacgatcaaa acaatcgaaa aaggttcatt cactattgat gaaacagtat gtgtcattga
    37621 aaccgaaaat ggtgacaaaa atttgacgat gcttcaaaaa tggccagtaa gacgtagtcg
    37681 gccaatcaaa gaaaaattaa acccggatgc acccatgatc actggacagc gagtgattga
    37741 tacattcttc ccagtgacaa aaggaggagc agcagctgta cctggtcctt ttggtgcagg
    37801 gaaaacagtc gttcagcatc aaattgcaaa atgggccgat gtggacatgg ttgtctatgt
    37861 gggatgtggc gagcgtggaa atgagatgac ggatgtttta aatgaattcc ctgaattgat
    37921 tgaccctaat acaggagaat cgttgatgga acgtacggta ttgattgcta atacatcaaa
    37981 catgccagta gcagctcgag aagcttctat ctatacggga atcacaattg cggaatattt
    38041 ccgtgacatg ggttatgacg ttgctatcat ggcagactcc acttcacgat gggcagaagc
    38101 acttcgtgag atgagcggac gtttggaaga aatgcctggt gatgaaggtt atccagctta
    38161 tcttggttca cggcttgcgg aatactatga acgatctgga agagtcattg cattaggatc
    38221 agaaggtcgc gaaggaagta taaccgcaat cagtgctgta tccccgtctg gaggggatat
    38281 ttcggaacca gtcacacaaa atacattgcg ggtcgtaaaa gtattctggg gtcttgattc
    38341 cagtttagcc caaaaaagac attttccttc gatcgattgg atacaaagct attctcttta
    38401 ttcaacagaa gtagggaagt atatggatca tatccttcaa gctgactggg catccatggt
    38461 caccgagggc atgcgtattt tgcaggaaga acagcagctg aatgaaatcg tgcgtttggt
    38521 agggattgat tccctttcag ataatgaccg tctgacatta gaagtggcaa aatccattcg
    38581 tgaagattat ttgcagcaaa atgcttttga tgatgtcgat acattcactt caagagagaa
    38641 acaattcaaa atgttgaaga tgatcttgac ctttggtgaa gaaggacgcc gtgcgttgtc
    38701 gttaggtgct tatttaagtg agatcatgga cggtaccgta tcgattcgtg aaaggatcgg
    38761 acgaagcaaa tatatcccgg aaacagaatt agaaaaacta gatcaaatta ttggtgaaat
    38821 caagcaaacg atgcaaacga tcgtttcaga aggaggcatg agcaatgatt aaagaatatc
    38881 gtacgatcgg tgaagtcgtc ggtccattga tggcggttga aaaagttgct ggtgtcaaat
    38941 atgaagaatt aatcgaagtc cgtatgcaaa atggcgagat tcgtcgtgga caagtattgg
    39001 aagtccaaga agataaagca atggtccaaa tattcgaagg aacaagtggt atcaatttac
    39061 gtgactcttc tgtacgattt ttaggccatc ctttagaact aggtgtatcg gaagacatga
    39121 tcggtcgagt attcgatggt ttgggacgac cgaaagataa cggaccagag atcctgcctg
    39181 aaaagcattt ggatattaac ggagaagtaa tcaatccagt agctcgggat tatccagatg
    39241 agtttatcca aacaggtatc tctgcaatcg atcatttgaa tacattagtt cgtgggcaaa
    39301 aattaccagt attttctggc tctggtcttc ctcataaaga attagctgct cagatcgcgc
    39361 gacaagcaac ggtactcgat tcatcagatg atttcgccgt agtctttgct gctattggaa
    39421 tcacttttga agaagctgaa tttttcatgg aagattttcg tcaaacgggc gcaatcgacc
    39481 gttctgtcat gtttatgaat ttagcaaacg accctgcaat cgaacgtatc gctactcctc
    39541 gtatggcttt gactgctgca gaatatcttg cttatgaaaa gggaatgcat gttttggtta
    39601 tcatgacaga catgaccaac tatgcagagg cattgcgaga gatttctgca gcacggcgag
    39661 aagttccagg aagaagagga tatcctgggt atctttacac gaacttagct actttattcg
    39721 aacgagcagg aagaatccgc ggactaaaag gatcggttac tcaaattccg attctaacaa
    39781 tgccagaaga cgataaaact catccaatcc ctgacttgac tggttatatt acagaaggac
    39841 agatcatctt gactagagag ctttacaaga gtgggattca gccaccaatt gatgtactcc
    39901 cgtctctttc gcggttaaaa gataagggaa ctggacccgg aaagacaaga gaagaccatg
    39961 cagctacgat gaatcagctt tttgcagcct atgcacaagg aaaacaggca aaagaattag
    40021 ccgtagtttt aggtgaatct gctttatctg atgtagacaa gatctatgcg aagtttgctg
    40081 agcggtttga aaaagaatat gtcaatcaag gattctacac gaaccgttcg attactgaaa
    40141 cacttgatct agggtgggaa ctgctatcga tgttgccaag aaccgaatta aaacgaatca
    40201 aagacgacat gctggacaaa tatctgccag aagggaagtg agttctgatg gcacgattga
    40261 atgtgaatcc gacccgcatg gaattgactc gattgaaaaa acagttgaca actgccacaa
    40321 gagggcataa attattaaaa gataagcaag atgaactgat gcgtcaattc atcctgttga
    40381 ttcgaaagaa caaccagctg cgtcaagaaa tggaagagtc tatgacagaa gcaatgtctg
    40441 attttgtatt ggcaaatgct tcgatcacgg aacctttcat cgaagaatta tttgttttgc
    40501 cggcggaaaa cgttgatttg tctatcgtag acaaaaatat catgagtgta aaagtacctg
    40561 tgatgaattt tgaatatgat gaacagctga cagacacacc tttggaatac gggtatttga
    40621 attccagtgc cgaactggat cggtcaatcg atcggtttac taccctgctt ccaaaacttt
    40681 tggaactgac agaaatcgaa aaaacttgtc aactgatggc gagtgaaatc gaaaagacac
    40741 gtcggcgagt caatgcatta gaatatatga cgatccctca attagaagaa acgatctatt
    40801 atattcgtat gaagctggaa gaaaacgagc gcgcagaagt aacaaggttg atcaaagtaa
    40861 aaaatatggg taccactgac taagtggtat ctttttttaa aagaggaaaa cgatgattaa
    40921 acaaagcaaa caaataaaga aacgtttgcc ggctgttcag cttctcgcat taggattttt
    40981 tctcctgatt ttagttggcg gcggcctact taccctacca ttttttagta atagtgggca
    41041 tgggacaaat tttatcgatg cgcttttcac tgcaacttct gctgtctgtg taacgggatt
    41101 gacaacattg aatacagcag agcattggaa cagtgctggt caatttttga tcatgctttt
    41161 gattgagatc ggcggtcttg gttttatgat ggttccgatt ctatgctttg cattgataaa
    41221 gaagaaagtc agtctcagca cacgtattat attaaaagaa gcgctgaatc tagaagaagt
    41281 atcaggtgtg atgaagctca tgctttacat cctaaaattg gcctttgtga tccaattgat
    41341 tggtacggtt gctttatctt ttgtatttgt gcctgaattt ggctggttga aaggtatctg
    41401 gtacagtttg ttccatgcaa tctctagttt ttgtaatgca gggtttgatt tgttaggaga
    41461 cagtttagcc aatcaccagc aaaatgttta tttgatcatg gtggtatctg ctttgatcat
    41521 ttctggtggg ttaggcttca tcgtatggcg tgatttactg gaatatcatc agaaaaagaa
    41581 gatgacgatt cattcaaaaa tcgcattaac cgttacaggt acccttttat taggcggatt
    41641 cgttgtgttc tttttcacag agcgcaatgg ggcaacgcta gtggaaggga atatgtttca
    41701 acgattggca aacacctttt ttatgagtgt gacacctaga acggctggat attattcagt
    41761 tgattacctc catatgagcc atgcaggtct gattttaacg atgtttttga tgtacatcgg
    41821 cggaacatct gggtcgacgg ccggcggatt gaaaacaact acgttaggtg tgttgctgat
    41881 ccagatgcgt tcgatgttga aaggacgtac gagagcggaa gcatttggcc gtaccatccg
    41941 acaaggcgca gtgttgcggg cacttacttt attttttgtt acgttgacgt tgtgtgtagt
    42001 cgccatcatg attttatcgg tgactgaaac gatacctaaa acttctggca tcgaatatat
    42061 tgcttttgag gtcttctctg cgtttggtac agtaggtctt acgatgggat tgacgccaga
    42121 cttgactgca atagggaaat tgatcattat agcgcttatg tacattggac gagttggtgt
    42181 attgactgtg gtcttctctc tgatgacaaa agcacaaaga acacaatcaa aatacaaata
    42241 tccagaagaa agtgtcatga ttggataatt tcatcttctg acagaaacct agtgatttag
    42301 taaaataaga aaataaaata atttttcttg cttcttggat actttaagtg caaaatgttc
    42361 gtttattttt tgaggcacta cccgttgttg aacctcgggt agtgcctctt ttttattgaa
    42421 aggaatcaat aagatttgat tcctttattt atcaataggt tataattcat atttttttat
    42481 tttttctttt acgatactta gtccagaagc taaaatttca aggttattag aggataggga
    42541 aattcgaacg aatgcatctg gttgcgcacg tgtagtaaag cgtctggaat gataaagtcg
    42601 gataccgtta gcaagaaagt cattctcaat tatcttaggg tcaatctcag gatcaactgg
    42661 aagaattcga tagtaaggga cacgattcgt tggtttttct actttgaaaa gagaatcaaa
    42721 aagctgattg gctctttttg taagacttaa tttagtttct aagatcctat ttgcttcatt
    42781 tgaatggagg atttgagtga tgacttccac atctaatcca gaagttttca catttatatt
    42841 aaaaattgct tgttctatct tgctttggac atgtactgga actacaagat aagcaactcg
    42901 caatccagaa caaataaatt ttgtcattcc agctaagtag attgtttggt taggtaataa
    42961 attttggaaa ggaggaatga tcgtatgttg atagtaagtt gttaaaaatg aatggatatc
    43021 atcttcaata acccatagac cttctttttc aatcacttcg gctaattgct ctcgtctttt
    43081 gtgagagatt tgaaacccta taggattatt acaagaaggc ataagaaaga caccatgaat
    43141 cttctttttt ctacattcct gaatcaatat atcaggtcgc attccttgct cgtcaaaatc
    43201 aattggaacg atctctaaat gatataactg agccaattca atgaaattag cgtatgtata
    43261 tcggtctgtt gctattctat cacctggaga gaaaagtgca gaaagtgtga tagccaatcc
    43321 attttgaaca ccggaaacaa tagatagaga ttctaaggac ggaatgactc cttgagtttt
    43381 taaccaattt gctgcagtag tcaattgaaa atttgtgcct ttgggatctt ggtaattaag
    43441 caaaccaaca agatttgggt tattactagt tgattgaata aacgggacaa cgaatttatt
    43501 acattcttca aaagaactaa ccagaccaaa atcaatgata tgttcggtta cttcattggt
    43561 agagatggtg atggactcaa agacattggg agaaataaat gtgccacttc caatattcgt
    43621 atacagcaaa cctttctcaa ttccatattt ataagccttg ccaacggtag taaaatttaa
    43681 atctaaataa tcagctagct ctctttggga gggtaactga gtgtttttct gtaatttatt
    43741 ctttaaaata tcttgttcta attgttgaat caacgatcga taataaggac gcttcaattt
    43801 ttgtttgtct ggtcgccaag ttaacgggta attgtcataa gaattaatag gcattttctc
    43861 cactccttaa aattgtatac catacaattt taccattgat tgtattgatt ttagaagtta
    43921 atcttttgtg aggagggaat tgtctatgaa aattattaat tttgaggaag tagtagcttc
    43981 ttttagtttt gaagaagcga ttcaagtaat gaaaaaatgt ttttttgatt atgaaagtgg
    44041 taaaatcagc caaaatcctc gattggtgga aattttacct gacggaaaag gcaaaaatat
    44101 gtttgcgatg atgcctgctt atttggggga gaacagatat tttggcacaa aagtcattac
    44161 cgcttttccg gataatcatc agtatcattt accttctcat ttaggagaga ttttcttgtt
    44221 tgatgctcaa aacggactac ccaaagcatt gataaacgca aatgcagtca cctggattcg
    44281 aacagctgct gtatctgcgc tggcaacaga ttacttagca aatccgcatg cagaaacact
    44341 tgctttaatt ggagctggtc aacaagcatt gtcgcatttg actgcgattt ctaccattcg
    44401 tcccatcact acagtgtttg tttacgatat tttagaagaa agacgaaata cttttatcgc
    44461 taaagctaaa aaacagtatc ctaaaataac gtttataaat gcaatatcag tagaagatgc
    44521 agtacgtcca gcggaaatca tttgtacgtt gacctctagt aaagaggcat ttcttcaaaa
    44581 agagtgggta agggaagacg cacatatcaa tgctgtgggc acatttactc ctgatacaag
    44641 agagataagt acatcgttga tggaaacttc tcaaatctat gtagatgatt actcagctgc
    44701 cttaacagaa agcggggacc tgcttatagc catggcagaa ggagatttag aggaatcttg
    44761 tattgttggt acattaggcg aattagtttc tgagaaaaaa acattttata aaggagaatc
    44821 gaggccgacg attttcgacg cagttggtct agctattgaa gatctttgtt gtgcagaata
    44881 tatttataac aaattagaga aaggggcttt aaaatgaact tagattatta cgtagttgat
    44941 gcatttgcag atgaggtgtt caaaggaaat ccagcagcgg tttatgtgct agaagaatgg
    45001 ctgccagaag gtacgatgca aaaaattgcc attgaaaaca atttatctga aacagcattt
    45061 acagtgaaga aaaaccaaga atttgagctt agatggttta cgccagatcg agaaatcgat
    45121 ctttgtggtc acgcgacatt agcaacagca tttgttttat ttaactatta caaaatacct
    45181 gatgaaacga ttaaattttc tactcaaagc ggtaatctat ttgtgacaag aattaaagat
    45241 tattattaca tggattttcc tagtattatg ccgaaaaaag tgccgatatt gtctgaatat
    45301 gaagaagcga ttggggctaa gattaaagaa gcctacttag cacgtgattt atttttcgta
    45361 ttagaagatg aaaaaacggt ggctaaattg cagccagatt ttacagctat caaaaatttt
    45421 gaattaggaa taggagtcat cgtgacagca gaaagtgtgc aaaaagactt tgtttctaga
    45481 accttctttc caaaattaac aatcaatgaa gatccagtat gtggatcagc acattctaat
    45541 cttattcctt attgggcaga aagaatgaat aaaacaaaat tgtcagctta tcaaatgtca
    45601 tcaaggggcg gggatctaga atgtgaatta aaagaagatc gtgtgataat aggaggaaaa
    45661 gcagttttgt tttctaaagg tacctcgttt atttgatttc tttcaaatga attaaaaata
    45721 aattggtgga gagtggcaac cttatagcta caatggttgt taaaaaaaga cgcaaacttt
    45781 ttcaatttaa tgaaaagttt gcgtctcatt ttaattattg gtgtttgttt tttattcaat
    45841 caagagtaaa atgccccata ctaactatcc tcggatgact tcgattttat aaccatcagg
    45901 gtcaatgaca aagtagtaag aaggcgcagt gccaggtaat ccttttaagt cagtaatctc
    45961 aagaccagca gctttatgtt gctcgtgtaa tttttctaga tcatcagctg ctatagcgat
    46021 atgaccatag ccgtctccta gatcatacgc accatgatca tagttgtaag tcaactctaa
    46081 ttcatagtca tccccaggaa gtgtcaaata aacaagtgta aatttgtttt ctggaaaatc
    46141 acggcggcgg ctttcttcaa aaccaaatgc cttttgataa aattcgattg atgcttcaag
    46201 atctttcact cgaacacatg tgtgagccat tttcatagat tcatctctcc ttttcctttt
    46261 actcaatcat aacaaaaaga agtaaggaaa tagttgattt gtgcttgttc atgagaaatt
    46321 tctcatgtaa aatggataga aaataaaact gttaaggtgg aaactattca tggataaacc
    46381 aatttttggt gaaaaaaagc aatttgctga ttatcaaacc cgttatgcag catatattgt
    46441 catagaaaga gatcagcaga ttgcagtaat cgaggctcca aatggtgctt tttttcttcc
    46501 tggcggggaa attgaaggag aagagacgaa agaagaagcg attgtccgtg agctgataga
    46561 agaaatggga attgtggcag aaatttcttc ttatctggga gaagctgatg agtattttta
    46621 ctcgaattat cgtgcaactt attattataa tccaggatat ttctttgttg ctgattcttg
    46681 gaagcgtata ggagaaccaa ctgaaaagac caataaaatt tggtgggtga gccctcaaga
    46741 agcgcttgtg aaattgaaac gtggtagcca ccgatgggca gtcgagagat ggatcgaaaa
    46801 aaataaaaac tgaaagaaag cgagtagatc agctttctct cagttttttt atttattaca
    46861 gtacatattt tttgattgct tcagctacac cgtgctcgtc gtttgatgaa gtgatgacat
    46921 ttgctgcttc tttgacaaga gaaacggcat ttcccattgc tactcctaga ccagcatatt
    46981 caatcatgga taagtcgttc tcgttatctc cgatagccat gatttcatct tgatcaatac
    47041 caaggtgctg tgcgagattc gcaactgccg cacctttact tgcttcttta tttagtactt
    47101 cgaagtaata gggcgcactt ttgaccgttg tgtatttatc acggaaagtt tgcggtaaac
    47161 gcgcaattgc tgcatctaaa atttctggtt cgtcgatcat catcattttg acgatgttca
    47221 tgtctggtgt catttcttca ggtgttcgat attttagcgg catcttgact aaacttgctt
    47281 cgtggatcgt ataagcgctg atatctcgat taggcgtgta gatcgctttg tcatcgatcg
    47341 aatggagatg actgccgaca cggcgtgcca ttacttcgat ctctaggaaa tcttcatgtg
    47401 tcagtccgtg acgggcgatg attttacctg atttcgtttg ttgtacgagt gccccgttat
    47461 aagtaataac gtaatcatta tcttgaaaaa ggttcaactc gtttaactgt tcctggacac
    47521 ctggtaaggg acggccagtg cataaaacga tcgaaacacc ttttgcaaca gcagcggcaa
    47581 tcgcttcttt tacttctttt gttacttctc ttttctcatt taataaagta ccatcgatgt
    47641 cgatagcgac tagtttgata gacataattt cctccaatat attttaattt atttcaatca
    47701 atgcgccatt acggatatgg ctggcaaatt cttgataagt ttcgtcaaat aaatcataat
    47761 gatcacgcaa ggacggatca accatttctt ttggaaagaa aaatcgctca tctccttgtt
    47821 cttgtcccgc taatgcagca acaagctgac taacctgtga taattctatc aaagttccgt
    47881 catttcgcaa aatctcgatt tgtgtgcgat gtctgccttg ttctgggcga taaaaatcat
    47941 aaggtagatc gtagctagaa ttcacagcag tgtaataaac aggattatat ccgacctttt
    48001 ctactaacaa agtgagttct tggactaatt ttgaatcccg gttgccagaa aaagtagcag
    48061 atttcagtgg tttacgattc aaaaaacgtt tagctaaatc atttaaaata ggatcagcag
    48121 attcagacca ttgagtaaag taagtgccca agaccccatc atcaagtttg aggtattctt
    48181 ctaaagtaaa gcttccgtca agaaatggca gaagtaattg agaatggagt tcaaaactgc
    48241 tggcatcttc ttgatacaac tcgtgtgcgc gatgaagcag atgctccaaa attacttcca
    48301 taccacggga aacaggatgg aagtacactt gcacatacat ttgatatcgg ctaacgatat
    48361 aatcttccac tgcatgcatg ccactcatgg aaaaagcgat cccgccttca taaggacgaa
    48421 tcacacgtaa tattcgggta agatcaaatg ttccatattc tgttccagtg aaataagcat
    48481 cccgaagcaa atagtccatt cgatctgcat cgatctgact agaaatcatt tggacaactt
    48541 gtgggttggg gtaagttttt tggatcacac tagctacctt ttcaggaaaa ccttcttcta
    48601 ctcggttaag aatctgatac acttctgttt ctggagaagt gatgatctct acagtgatgg
    48661 cttcatgatt cgtatggaag atatgttcaa atgtatgaga gtaaggacca tgaccgacat
    48721 catgcaacag tgccgcacat aaagtcacca gccgttcctg atcatcccag cctccgttgc
    48781 ctattttttc ttttgaaaaa tttcgagaaa agatatcgca gattcgtctt gaaatctcat
    48841 aaacgcctaa tgaatgagta aaacggctat gttctgctcc gtgaaaagtg aaagaagaag
    48901 tgcctaattg tttaatgcgt cgtagacgtt ggacttcttt cgaattgatc aaatctaaga
    48961 ttacttgatg ctggacatgg atatagttat ggactggatc acgaaaaact ttttccatag
    49021 gcagcatttg atatttataa ggggttgtca taatgtagct cctttatgtt attacgttgt
    49081 tccattctct ggatttggtt gttccattgc tccagctgga tttctttttt tagccagtcg
    49141 gtagaatcct gtaaatcact tgcagaatac agcttttcaa aagcttttgc aaatgcttct
    49201 ttgacttctt ttacaaaaag gggttggtca agcagttctt caagtgttgc cattgtagca
    49261 ggatccactg caggatagcc atttgtacca aagtcttctt ttaaagcaga ttgataaaat
    49321 cgcttcatga cttgcccacg tttttcctga ttaccgttga cacttaagta catcataaca
    49381 gaaatcccat ttttgatccg acgctgagcg ataccggcaa attttttgcc atggatgctt
    49441 aaatcataag ttccaggaca ataagaatgg gtgatttcga atgcttgtat cggtgccttt
    49501 ggaaaagcgt atttagtcaa taggagcatt tgctcgtaag cgtcatctat ggttattttt
    49561 tttgtgtcac tttgggggaa aatcaaagaa atattcaaaa ttccttcatc tgatacgatc
    49621 cctaatccac ctgaatttct gatcactgga gcgtaacctg catcttccag tgtttggatc
    49681 ccgtcactta gaaaaggagt tcgaacatcc ttcataccta agatgactgt gcagtcatat
    49741 tgccagaaat gtaaaaagat ttgctggtgt tctttagaat attccgttaa tatatctgtt
    49801 aatgcaaaag gggaaaagtc gtttttttga taaatgtgct gatctagtcg aatcgcaggg
    49861 ttcattcaac cggctccttt gtataaattc atattttcta caatattata ctctaaaatc
    49921 aaagagttgt tttggaaaaa aggttttctg taaaaatgga caagttggtt gacaaaacaa
    49981 aagaaactag gaacaatgta gctatgagaa gtaaaggaga atgaataatg gaattatcac
    50041 aaggaacacc gatttcaatc aaattgcgta caaaagtaaa acagcaaggg gaagtgcaag
    50101 atttttattt tgatttgaaa gggcaaatgg tgacaattgg cgatacgctg tacatacgtt
    50161 ataaagagat tcaggaggag acgggcgatg agattcctgt aacgatcaaa ttagttccag
    50221 atggtcatgt acagctgatc cgtgcaggag aaatgcggat gcgtttgaaa tttggctata
    50281 aagaacggtt agaaacatcc tatcgaacac catacggtat gatccagatt gctactttta
    50341 cgaaagaact gcatgttagt ttgaaagacc gtcccacagc agggaaggtt cgaatcgatt
    50401 atgatctgtt catgggaccg gaaaaaatcg gcgaatatta tttgaccttg gattttacag
    50461 cgtaaatata aaagaaaagt ttgatttttt gtgtctgatt ttgtatgata aggagtagac
    50521 tgtgaaagga cgtgtcctga ttggaaatta gtgtatttga aggtgcaaac aaaagcgaat
    50581 tatcaatgat cgaagtagca cacgcgatct tagaacaacg tggcgatgtc atggattttt
    50641 ctgatttagc taaccagatc caaaactatc ttgggaaatc agacagtgaa attcgtgatt
    50701 cactcgcaca attttatact gacttgaata ttgacggcag ctttatctca ttaggggata
    50761 atcgttgggg cttgcgttca tggtatgcta tcgattcgat cgatgaagaa gtaaaccatg
    50821 gaatggacga agaggatgaa gatactccac gtcgccgtaa acgcaagaaa gtcaacgcct
    50881 ttatcaatga tgatgaagac gcgatcgatt acaatgacga tgacccagaa gatacagatc
    50941 tgacagaaga agatgacgat gatctgtttg atgacgatga tgatgaagat gaagaaatcg
    51001 ctgcttataa ttctgacttg caagaaatcg gagcagatga cccgacagat gatgaagaag
    51061 acttgccagg aaatatcgaa gaagatttaa gtatcataga cgaagacgat gatgatgaag
    51121 attatgaaga ggaagaattt tctgactctg aaaaagaata aacattattt tcttacgaga
    51181 ggttgtgaca gagacgatca gcctatcaga ataaggaggt gtgacacgat ttttgtcaca
    51241 ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa aattgaaact
    51301 tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa tgatttttac
    51361 tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg gactcatcga
    51421 aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata aagaagaggc
    51481 cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa aagaaaaaga
    51541 gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat tagatagttg
    51601 gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc cccaacagtt
    51661 acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag acagtccgga
    51721 aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa ttgcgactat
    51781 cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga atgctttacg
    51841 tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa aatttaaaaa
    51901 aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag gagatccgcc
    51961 agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag agacgtctcc
    52021 aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt ctttttaata
    52081 gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt gaattgatct
    52141 taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg aagatcaata
    52201 cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac tttcgcgaag
    52261 gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg gatcattacc
    52321 aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg attgaaaggc
    52381 agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca atcgacaaga
    52441 aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat ctatctgttt
    52501 tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca tctaagtact
    52561 attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt gttacgccta
    52621 cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct caatacgaaa
    52681 ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg acaaaaacga
    52741 ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac tagcagtttc
    52801 aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa gagatatagt
    52861 gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt ttttcgaaat
    52921 tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt cggagcattc
    52981 gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa ctgaaacagc
    53041 taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt catcacaatc
    53101 aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg caatcagctt
    53161 tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa aagaaaaaaa
    53221 cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg aaatcgtgaa
    53281 gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc agaatctggc
    53341 cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg agatcaaaaa
    53401 agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca cgaatgtagg
    53461 agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag attggaagac
    53521 aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg gggcaattat
    53581 tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga tcgattatct
    53641 gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc caacggctgt
    53701 cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac aagtatcttt
    53761 aataaataac acattttaat aagtagttga acacataact accttacttg caaaggctta
    53821 acaatatcta tacaggtaca acaaaaaact gtccaccaat ttt
//
LOCUS       DAYYCW010000009        49517 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000009 DAYYCW010000000
VERSION     DAYYCW010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.009"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            205..1656
                     /locus_tag="ACHAYA_000516"
     CDS             205..1656
                     /locus_tag="ACHAYA_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8604348.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            2005..2637
                     /locus_tag="ACHAYA_000517"
     CDS             2005..2637
                     /locus_tag="ACHAYA_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM8604349.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3225..4664
                     /locus_tag="ACHAYA_000518"
     CDS             3225..4664
                     /locus_tag="ACHAYA_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8604350.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4959..5177
                     /locus_tag="ACHAYA_000519"
                     /pseudo
     CDS             4959..5177
                     /locus_tag="ACHAYA_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            5426..5641
                     /locus_tag="ACHAYA_000520"
     CDS             5426..5641
                     /locus_tag="ACHAYA_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604351.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5858..6114)
                     /locus_tag="ACHAYA_000521"
                     /pseudo
     CDS             complement(5858..6114)
                     /locus_tag="ACHAYA_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6301..7212)
                     /locus_tag="ACHAYA_000522"
     CDS             complement(6301..7212)
                     /locus_tag="ACHAYA_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8604352.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7231..8235)
                     /locus_tag="ACHAYA_000523"
     CDS             complement(7231..8235)
                     /locus_tag="ACHAYA_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8604353.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8232..10355)
                     /locus_tag="ACHAYA_000524"
     CDS             complement(8232..10355)
                     /locus_tag="ACHAYA_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8604354.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10360..12807)
                     /locus_tag="ACHAYA_000525"
     CDS             complement(10360..12807)
                     /locus_tag="ACHAYA_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8604355.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12794..13183)
                     /locus_tag="ACHAYA_000526"
     CDS             complement(12794..13183)
                     /locus_tag="ACHAYA_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8604356.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13243..13746)
                     /locus_tag="ACHAYA_000527"
     CDS             complement(13243..13746)
                     /locus_tag="ACHAYA_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8604357.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13759..13983)
                     /locus_tag="ACHAYA_000528"
     CDS             complement(13759..13983)
                     /locus_tag="ACHAYA_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604358.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(14087..15997)
                     /gene="ltrA"
                     /locus_tag="ACHAYA_000529"
     CDS             complement(14087..15997)
                     /gene="ltrA"
                     /locus_tag="ACHAYA_000529"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8604359.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16743..16880)
                     /locus_tag="ACHAYA_000530"
     CDS             complement(16743..16880)
                     /locus_tag="ACHAYA_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8604360.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(16877..18061)
                     /gene="mobT"
                     /locus_tag="ACHAYA_000531"
     CDS             complement(16877..18061)
                     /gene="mobT"
                     /locus_tag="ACHAYA_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8604361.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18320..18610)
                     /locus_tag="ACHAYA_000532"
     CDS             complement(18320..18610)
                     /locus_tag="ACHAYA_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604362.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18600..19742)
                     /gene="dcm"
                     /locus_tag="ACHAYA_000533"
                     /pseudo
     CDS             complement(18600..19742)
                     /gene="dcm"
                     /locus_tag="ACHAYA_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19818..19982)
                     /locus_tag="ACHAYA_000534"
     CDS             complement(19818..19982)
                     /locus_tag="ACHAYA_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604363.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(19975..20649)
                     /locus_tag="ACHAYA_000535"
     CDS             complement(19975..20649)
                     /locus_tag="ACHAYA_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8604364.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20727..22067)
                     /locus_tag="ACHAYA_000536"
     CDS             complement(20727..22067)
                     /locus_tag="ACHAYA_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8604365.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(22150..22485)
                     /locus_tag="ACHAYA_000537"
     CDS             complement(22150..22485)
                     /locus_tag="ACHAYA_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM8604366.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(22495..22869)
                     /locus_tag="ACHAYA_000538"
     CDS             complement(22495..22869)
                     /locus_tag="ACHAYA_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8604367.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22882..23196)
                     /locus_tag="ACHAYA_000539"
     CDS             complement(22882..23196)
                     /locus_tag="ACHAYA_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8604368.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(23309..26536)
                     /gene="fss3"
                     /locus_tag="ACHAYA_000540"
     CDS             complement(23309..26536)
                     /gene="fss3"
                     /locus_tag="ACHAYA_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8604369.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27393..27647
                     /locus_tag="ACHAYA_000541"
     CDS             27393..27647
                     /locus_tag="ACHAYA_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604370.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            27811..28728
                     /locus_tag="ACHAYA_000542"
     CDS             27811..28728
                     /locus_tag="ACHAYA_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8604371.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            28721..29503
                     /locus_tag="ACHAYA_000543"
     CDS             28721..29503
                     /locus_tag="ACHAYA_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8604372.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            29504..30184
                     /locus_tag="ACHAYA_000544"
     CDS             29504..30184
                     /locus_tag="ACHAYA_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8604373.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            30177..31073
                     /locus_tag="ACHAYA_000545"
     CDS             30177..31073
                     /locus_tag="ACHAYA_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8604374.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            31285..33207
                     /locus_tag="ACHAYA_000546"
     CDS             31285..33207
                     /locus_tag="ACHAYA_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8604375.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            33208..33654
                     /locus_tag="ACHAYA_000547"
     CDS             33208..33654
                     /locus_tag="ACHAYA_000547"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM8604376.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            33670..33984
                     /locus_tag="ACHAYA_000548"
     CDS             33670..33984
                     /locus_tag="ACHAYA_000548"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFM8604377.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            34010..35059
                     /locus_tag="ACHAYA_000549"
     CDS             34010..35059
                     /locus_tag="ACHAYA_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8604378.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            35071..37674
                     /locus_tag="ACHAYA_000550"
     CDS             35071..37674
                     /locus_tag="ACHAYA_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8604379.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            37791..39440
                     /locus_tag="ACHAYA_000551"
     CDS             37791..39440
                     /locus_tag="ACHAYA_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8604380.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            39756..40691
                     /locus_tag="ACHAYA_000552"
     CDS             39756..40691
                     /locus_tag="ACHAYA_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8604381.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            40684..41454
                     /locus_tag="ACHAYA_000553"
     CDS             40684..41454
                     /locus_tag="ACHAYA_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8604382.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            41869..42852
                     /locus_tag="ACHAYA_000554"
     CDS             41869..42852
                     /locus_tag="ACHAYA_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM8604383.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            43173..44210
                     /locus_tag="ACHAYA_000555"
     CDS             43173..44210
                     /locus_tag="ACHAYA_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8604384.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(44299..44622)
                     /locus_tag="ACHAYA_000556"
     CDS             complement(44299..44622)
                     /locus_tag="ACHAYA_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8604385.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(44609..45316)
                     /locus_tag="ACHAYA_000557"
     CDS             complement(44609..45316)
                     /locus_tag="ACHAYA_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8604386.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            complement(45615..47039)
                     /locus_tag="ACHAYA_000558"
     CDS             complement(45615..47039)
                     /locus_tag="ACHAYA_000558"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8604387.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            47214..47927
                     /locus_tag="ACHAYA_000559"
     CDS             47214..47927
                     /locus_tag="ACHAYA_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8604388.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            48076..49407
                     /locus_tag="ACHAYA_000560"
     CDS             48076..49407
                     /locus_tag="ACHAYA_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8604389.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT        15324 a         9310 c         9299 g        15584 t
ORIGIN      
        1 ttgaaaatgt tagattattt atttcatcct agttttagcc aaccctataa ttttatttta
       61 aaggcagtaa caggattaaa cattgaaaca taatcgttac taaatgaaat caagaaatat
      121 actgtattac tcaattcatt gaaaatattt atcataattt ttaatagaaa acactattaa
      181 agagaaaaaa gagaggtaaa tcaaatgaaa aaatctttaa aacttgcatc agcagttata
      241 ttaatgtccc aactccttat agcaacacct ttccatgtgt tggcaagcga aaattcaaca
      301 tacaaaattg caaaagaaag aaccgagacg actgaaatga gtaattccga tgtaacagca
      361 gagagtagtg aacttttgac aacttcatca agtgaaagta gtacgacttc aaatgccgag
      421 tcttcaacaa cttcatcaag tgaaagtagt acgacttcaa atgccgagtc ttcaacaact
      481 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc gtcaagtgaa
      541 agtagtacga tttcaagtac cgaatcttca acaacttcaa gtagtacaag tgaaataact
      601 tcttcgaaag tgacaaaacc aaaccatata gaatcttcaa aaaaggaagc gccaaaaacg
      661 gaaggacaaa caaaaacttt ttcgacacca cagcaaatag aatcaacaga atctttccaa
      721 tcacaaccaa tactatctgg taatgaagaa attcattttg acaaaaatga atctgtagaa
      781 aattttatta tgaaaattgg tgagtctgcg agaaaaattg gtcagaaata tgatttatat
      841 gcttctgtta tgattgcaca agcgatctta gaatctgcca gtggtcaaag ccagttagct
      901 caggcaccga attacaattt atttgggata aaaggaactt ataatggaaa ctttgtcatt
      961 atggtaacaa acgaagattt gggtaatgga acgctttata cgacccaatc aaaatttaga
     1021 gtgtatgaaa actatgagga aagctttgaa gattatgcca aattattaac taagggaatt
     1081 agtggaaata aagattttta tgcaggagca ttaaaagcta atagtaaaac atatcgagaa
     1141 gcaactaaat tccttacagg acggtatgca acagatacac aatactattt gaagctaaat
     1201 gaattaatta aaacctacga tttaaccaac tatgataaag aagttaaagg tccacaaatg
     1261 agtaagactg gataccaagt gcctattcat aattattcaa taaccagttt gtttggaaat
     1321 cgtggaaatg aatttcaccg aggacttgat atggcagcta cacagggtga gccgattcat
     1381 gcagttaaag ctggtaaggt tgttaaagca gagtttcact actcttgggg aaattacgtg
     1441 gtgatacaac acgaagatgg atcaaccgcg ttatatgctc accagcaaca atacctagta
     1501 aaaactggag ataatgtttt gcaaggacaa ataattggct atgtaggaag tactgggaat
     1561 agtactggcc cacaccttca tttggaaatt tgcaaagata gtagtctttc tcagagcatg
     1621 ttagttgatc cgaaaacctt attattcgga gactaaaaaa ggtaatcaat ctgacaaagt
     1681 aacaagaaaa tggttggatt cttagacctt tgatcaggat attgagactg gaattgatat
     1741 ctcgattacg atgggtttga caaataaaac aagtccatta tcgaatatcg agtgtattat
     1801 tgccgtcttt atgctcatat tttgagcagg tttcactaga tgaaaacaat ttatctatct
     1861 tgaggatttc gtgtacgtat cgttcagtct tgtattgtaa attagtcata aaatcagact
     1921 aagaaaaatt ggaaatattt ttttctgatt tattaaaagt taattgaaat tatatggtac
     1981 ttctttcttg ggagggtagg tgcagtgaag gaaagatatg gtcaccttac tgttgttgag
     2041 gagattactc ataatggata taaaaaatat cgttgcctat gtgactgtgg gaatgaaacg
     2101 attgtatatc gatcaaactt actatcaggt agaaccaaaa gttgtggatg tggaaattat
     2161 aaaaataggc ataaatataa aaatttgaca aataaaactt ttggtaagtt agttgcaatc
     2221 aagccaacta aactgcgaag tgaaggaact gttgtttggg aatgtcaatg tctgtgtggg
     2281 agaaaaaagt tagtatcggc aaagcgactt ctaaaaggtg atgtaaaaag ctgtggatgt
     2341 ttgaaatcaa aaaagcatca cattgaaaat aaaaaattcg gattacttac tgcaaagtat
     2401 cctttggaca ataactgttc atggaaaaca aaatgggttt gtcattgtga gtgtggcagt
     2461 atgtgtgaga taagttatag taacttattt tttggacata ctcaaagttg tggatgttta
     2521 aaaaggcaag aaacaaggac atgtgttgaa gggacagtaa tagagtgtct aacgtcaaaa
     2581 ttaccgaaaa ataatacaag tggtgttaag ggagtttatc gttctaaaga gaaatagatt
     2641 gcgtatataa ctttaaaaaa aaagaggtac tatttaggat cgtatgattc tattaagagt
     2701 gcagctcaag ctagaaaaga tgctgaaaat aaattgtttc aacctttgat tgacaaatat
     2761 cgaggtaatc attaattatt gtggataata attaacagtg atatataaac atctataata
     2821 tattattcat actattgata tttagtataa tcctatggaa ttgaatatca tagcaatgta
     2881 tttgaaaagc aaattggaaa acaaataggt gcaacaccat aaatatgaaa aaattgggaa
     2941 aaagtagaat ggaaatttgc ttacgtctca cgagatagct cacactatta atctgaagaa
     3001 caatgaagtc gacttttaga aattgctaga agaaagtaaa gcgaattatc actagttact
     3061 atagagtaag ttcaaataaa gcatctccaa cttacatggt tacatgagga tatcaaatcg
     3121 aaattactca agcttcctat ggctagagtc gcgagaagtt gttgggcagt agacataggt
     3181 tatattgttc ttagctacac gatcattaga aaggaaatcc tattatgtat agttatcaag
     3241 aagatgcaaa agtccaaaaa aatcgctggt ggattttgat tgctatatct atgtttacct
     3301 tcatgtccac attggatggc agtattgtca atattgcact acctaccatc tcagatgata
     3361 tgggcatacc aatgaatcaa gcggaatggg tagtgtccgt ttatctgatg attgtctgtt
     3421 cttgtttgtt gctgtttggt aaaattggcg atagccgtgg gaaaatcaaa gtatttcgga
     3481 ttggtacgct gatctttgtc ttgggatcgc tgttgtgtgg gttcaatcat tcgctaggta
     3541 ttttattgtt cgcacgggtg gtacaagcca ttggtgctag tatgactatg gctacaaatg
     3601 ccggaatcat cacggaagtc tttcccatac atgaacgtgg tcgggcattg ggagcaatag
     3661 gtgcgtttgt ttcattaggc tcgattgctg gaccaggtct tggcggcttg attttgtccc
     3721 aattttcttg ggcgtacatt ttttggatca atgtaccggt gggaatcgta acgatgttga
     3781 ttgctgaaaa attcttacca aaagatatca ccatgagcgg ccatacaatc gatggagggg
     3841 gctttgctgc cattgcggct acgattatca gcttttttgg agcaatcttt ttggggcaag
     3901 aaagtggctt tttccatcca gtcccccttg tactgttcgt aatttcagtg attgcctttg
     3961 tcagcttttt aaaagttgaa aagaaagcag cctctccatt gatttctttc ggtattttta
     4021 agaacaagat ttttaccatg agcttgatta cagccttgtt gattttttct tccaacttct
     4081 ttgtcaatgt agtgattcct ttttatctgc aaaatgctcg cgggttgagt cctagctatg
     4141 ctgggctgtt gatgatggtc tttcctttga tgatggtcgt cggggcgcct ttaagcggct
     4201 acctgaccga ccgggttggc ccggagattt tggtattagt aggcttgggc ttattgtcgg
     4261 taacccaggt catgtatatg tttttgtcag tggactctcc tttgtggcta tacattgtcg
     4321 ctactgccat catgggtgcc ggcaatgcct tgttccagtc ccccaataat acgatggtta
     4381 tgagctcggt cactatcgaa aacttgggac tggcaggctc gatgaattcc tttgcccgaa
     4441 atttggggat ggtcagcggg atttcgcttt ctacgacggt cttatatcaa gcgatgtctg
     4501 ctaagatggg ccagcaagta actaactata tcgcaaatcg gccggatgtc tttttatatg
     4561 gaatgaagat taccttttta ggcagctttg ttttgtgttt tgttgccttt ttcctgacta
     4621 tctggcgtat caaaaaacaa ggaggtattc agaaaaatgt ctgaaaaaac cactgttgaa
     4681 gaatttttgg aggcctgcgt atgggtgagt gggatttcca tggatgatgg aagatgaagt
     4741 gaacttctaa aagccgaaaa actcaagtat tatcttcaaa atactagtgc tcaatgagat
     4801 gcttgatttg atcacaagaa gactgcgaaa gccgctggca accgcaagct aggtatccca
     4861 ccgatggact acgttttaga ggctactggt tatgaatacg gagccaacgc tctcatctgc
     4921 ataagaatac atcacccaga ttatgtaaag attttcgata cttctgtcaa aaaatcatta
     4981 agatagataa atgatttccg tctagtcaaa ttggttcaga atgtagacac acagatggta
     5041 agaagtcact cacaattcga gaatggactt gtcctatttg ccgtatacat cataatcgaa
     5101 acgtcaacga tagtatcaat attttgacca agggctatga atacaagcat tgccttagaa
     5161 aacatcaaga accttaggaa caacggggat agcttggtta ataagagata ccgctgttta
     5221 ggcttgcaag tatactcttt tatcaagaat tccgtggctt tagtcatgag aggcttaata
     5281 ctatgaaaat tttctttagc atttttgatt taggttcctt ttctaattat aataagatga
     5341 ccactacagt aacctactga aatatataaa cagccactgt agtaccattt tcaaagtagt
     5401 ctatgagagg agaatcttca ttcttatgaa cgctggaatt gcggatatga atttaataaa
     5461 aaaaaccctt aatgatttta cttccaattc aatatcaaaa ggtactggta tcaatttaag
     5521 cacaataaaa aagctgaaaa gtggtgaaag atcagtggaa aagctgaatc ttttagatgc
     5581 tataaaaata acggaatttg ctatgaaaaa tggaaaagct gagattgaaa tatggagata
     5641 attatcgtct agtgaattac ttgccactta gctgaattta acgatccttg gtgcttgaag
     5701 ggggagattc ttgggaatag agcatacttg caagcctatg tctttactaa caacgatatc
     5761 tcttattaac caagctatcc ccgtagttct aaggctttta cttttatcta agcaataatc
     5821 gtattcaaag gaaatttttg acccttagaa agtctcattt tatcagacgt gagcttaggc
     5881 attgctgaat tttgttaatt cggttttagg ttgtgctttg agcataatat gaaagtattc
     5941 tttatcacga tttcattcat ctaaggtaat gtgatgtgat tctgaaattc tttcaaaggc
     6001 agtttttgca taagctgaca ttacatcatc aatgacatgt ttacaatgtt tcactactaa
     6061 aataaggggg taaccaagaa gaaaaactga atgattattt gtatctaatt ccatggtata
     6121 tcaaccattt cctatataga ctgattatga catggattaa aataaaaagg cactatgtct
     6181 taattgtcgg ttttcgagtt accgaatggc attcatcacc cacctataga ggatgggcga
     6241 cttctgcctg aaataagtta cactacttat aacttctaat aaaattagat tgagtcatta
     6301 ttatttaaca tcaacgattt tccagtttcc atttttttgt aatactaatt tgtactgaaa
     6361 gtagctagtg gtttttgata cattatcaag gtattttatc tttgtactca ctagaaaatt
     6421 ttttccgttt tgtagataaa ctggattctc taactccgaa aactttaagt caatattgat
     6481 tggtcgcatc gcaccatttt caacataata ctccaattct ttttcagaag ctgatggata
     6541 aagtttaaaa aatgtttcta aaaaatcggt gatttcttcc gttgttttgg catcaatttc
     6601 tgaatcgttt tctacttgtt ttgcttcata ttttgccttg tctggctttc caatcattgt
     6661 tggcaaacta gttactaaat gattcctgtt ttcatcttca aagattgtca ctcgatatgc
     6721 agaagaaatt gattgcgtct tttttccctc tgtaatatgt tgctccacta aaaatgaaac
     6781 gacaaattct tccgagtgtt tttcaacatc tagaattttt acagattgaa cttcggaact
     6841 cgtgggaata tctgctcgaa ccatatcttg gattaaagcg agaccgtttt ctgtaaggta
     6901 ctgttcgaga ttactcattc tcttttcaat tgcttctttg ttatttttcc atgaaaaata
     6961 ttctttcgca aaatcctctg taaaactttc aacgccagaa gtattcacta ccttctcttt
     7021 tataaccact ttttcatgag ttgtatgttg atcaatggcg gtgaaatttt tataaacgcc
     7081 aaaagcaaga ctacaagaca ataaaagcca aagggcaagc accatttttc gatgcttacc
     7141 cacggataca actcgttctt ttttcttttt tgggctcttt tccttttgat tccgttcaat
     7201 ttttatcttc attctttttc ctcttttctc ttatttgtat cggccagctc cgataagatg
     7261 ctgccgccaa tagttttccg ttagattcgt ccaccccact ggatctcctg catgaaacat
     7321 gcgattctcc cctgcgtaaa tgcccacatg ggtcacataa gtaccggcat cataggttga
     7381 atgaaagaaa actaaatcac ctggtttggc atcttttaga tcaatatgag tcatggcatc
     7441 atattgatcc tgagccaccc gaggaagttt caggtcaact ttggcataac accattgggt
     7501 caagccacta caatcaaacc ccgtttctgg acttgagcca ccaaacacat aggccgttcc
     7561 ttcatactta aatgcctcat caaaaatttc ttggactatt ttgtcatcaa actgagttgt
     7621 agttaggtat tgcttcacta ataaacagta aaacatattg ccataattat accgccaccc
     7681 accattttcc ttaatcgcga ttgggttggg gtaatcgact ttttgaccac ctgatttttc
     7741 ctttgaaaaa gattcagcta aagcaaaact atgcttcttc ccgtttttcc cgacatgatc
     7801 tagatacccg ccaccatagt tataggcctg aataatacta tcttgatcac accctagttc
     7861 tttggaacgc ttaaccaaag acgcaaaata gagacagcct tgtttgatgg atgcctcggt
     7921 acttaatgaa ttaggtggta atccggctga ctcagaactt tgcataacgt cttccattgt
     7981 gccaccagat tccacttgaa taatcgccaa aagaattgga atatactctg ttatatccga
     8041 ttctttggca tacttttcca ccattgcacg gtgtttcaat acttcttctg agacactagt
     8101 tccgccagga gaagtgccag aactaccatt tccctcactg tcttcaccaa acaacaaacc
     8161 cacacacatc aaaataccca taaaagagat ggtcagaaaa gttaatgaaa taacacccac
     8221 tttcttcaag ttcatttttt ctcacctgac cttgattttg gacgatggtt cattcttttt
     8281 tgtacttttt gattggtctg ctttggcaga gtggacctct gtttcacttg ttggaaagaa
     8341 tttttcttac ttgctgattg gcttttccct tttgaaatga tttcttgact agttagtttt
     8401 cgtttgattt cgggattcag atttgctctc acaatccgct ttggtgcttc ttttttcagc
     8461 atcatatctt tctgaggttg agctgcttta tctattggaa tattcgccct tctttttccc
     8521 tgacgatttg ttgcttcact cagtatcttg cgtttttcgt ccattttcgc atttcttcgt
     8581 tgtcgttgtt tttctcgcaa atccactcga tccgcttttt cttggaacag tccgcgcttg
     8641 aattcttctg gtgctttttt cgacttttcg attcctttat ggagattgta tttcaaattc
     8701 gttggcgtat tccttacctg atcctttact tgtttagtct tatctttcac caaagcttgg
     8761 gtatccaaaa ctttgccgat ttttctccca gccaaattca ttcgggattg tttcttatta
     8821 tttgaaactg gattctcctt agttaagtca gatagtattt cgtgatcgtt cggtaatcgt
     8881 gaattctttc gtagagaaga accggatggc ttaaatttgg attttgattg ttcctttgcc
     8941 aacttatttc cgactgctgc accaccacca aaagccaaag ctcgtcccac atttcgattg
     9001 agttgccgca attttcgatt cattaacatt tgcggttttc gcatgacgcg tcttcccatt
     9061 gactggctat cattactctg gaggttgaac atactcataa tgtcgcccaa tttgaaatag
     9121 ataccggcaa aggtcacaat ttgcagaaaa gcaaccatga aaaatggata ggttgccgaa
     9181 atattgaaaa acatcgtgga gatgctaaat gcagtcgtaa tcaccaaagt aacacccgct
     9241 cgcatcataa tcgtattgaa taaacgaata atcgccttct ttcctaaact ttcataagtc
     9301 ggcaacatgg acaataagaa actaattggc agaaacatcg caaagataat gaagagaatt
     9361 tgcgagaaca acataatccc tgttagaaga aaaacaaaga aggaaatgcc aatattgaac
     9421 aataccaaaa agaccacggt ccctaaacgg ttcatggtct tggtaatact gagattcgca
     9481 ttgtcattgt cttcaatctc tgccttgacc gcttcttccc gatctttgcc tttgttttca
     9541 tctggactca cagatagaat cttgttgacc cggtcttcac caatttcttc tatgttggaa
     9601 tcatcaaatt gcagtagtaa ccaaggttgt tggacttgaa tcgaaaacaa actatcccga
     9661 atcaaatcca cactatcttt tccttggctt tctgaattgg gtacaacgat ctcggttcca
     9721 agggtaaggg ccgcttcact aacatctgag ctaaagtcat tgatttttgt gatataggta
     9781 ggcgcataag caatgaacga acccgatagt agaaaaatca tcacaaaatt aatcacggct
     9841 cgaactgctt tcgtggtttc tcgttttaat aatccggtat acgccacata cacccccaga
     9901 gctaaaatca tcaataatag aaacccaaaa taaaatccgg aggtctggag tccattttca
     9961 gtaataccag ccaatgtttg gatattcttt ccaatacttt cagccgtatc cgaaatgaaa
    10021 tctaatttgt aagcctcttg aaccacataa cccgtggcgt tcgacaaata caaactcacc
    10081 gtccaaatga aattagtaat ggcataaaga ccatacatca cgcttttccc taagccatca
    10141 ccccaattcc aaggcaacca atcccaagag ctatccacaa agaaatctag ttgatagttg
    10201 ttcagtgaat atttggaata tagatttcct gcttcaactg tatcatcaac tagccctgtc
    10261 gcctcggcta ccgtaccaac taaactcaat agaactatca gagtgagtat gctaataaaa
    10321 acgattccca caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac
    10381 aggtggacgg gtatcaaagg catggaagag ttcttcaaag actggatgta cctgaaccac
    10441 gcctacccga ccatacaaat cctgcatcaa gcattggcca ttttccaaat ctcgtaggcg
    10501 tttttgattg ctttcatctt ctcgatccaa gccaaagaat tccaaggttt tcttgatttc
    10561 atgaatatcg gtactgcgaa acgcaaactt caacccgata ttattcttca agcgttcatc
    10621 cagtaaatca tcactatttt gcgtgacaaa gtaaaccccg gcattcatag cccgcccagc
    10681 acgaacgagc ttattcgata atgttttccc ttgcgctact tgaagaaaac tccacgcctc
    10741 atctaagtct acaattttga agatttctcg attactatgg ataaagtcca aggcaaaagt
    10801 ggaaatgacg attagcatgg aaacactaag caattccatg gtggtatatt cttcaaaagt
    10861 ggtttctgca tctggtaaaa ccaaatccgc tacttgaatg atatttaact gcctatctaa
    10921 actaatcgac tgggtaatcg aaccatctga gaaaagcaaa tgggcaaaat cataatcggt
    10981 aaaggactcg atgtgatccg cgatattttc cgcaactggt gagccttctt ttcgaagctc
    11041 attaattact tgtaataatc cccgttgatt gctttgggta accgtccgaa tggccttacg
    11101 caacactgga aacttctccc catctcgact agaaattcct gttaaaaagg tcagaatatc
    11161 aattgctaga ctttcagaat ccttcttttt ctccataatc acataaggat caagtaatcc
    11221 ttgatttttt ggcttactag ttaagttgag aatattgatt tcatccgcaa tttctggcaa
    11281 ggtttctttc cagccacccc gttccccttt tggatcgaca atcacagctt gtccaccaaa
    11341 aaggacggcg taataaacta agagattgtt actaaaggac tttccgccac cgagtgaacc
    11401 gaggaatgcc gctgctagcg cattggtaac tgaatccttg accccttgtg aggccaatgc
    11461 cggtttcagg taaacattgc gccccgtatc tacgttataa ccaaagtaaa tgccttccaa
    11521 ctcccctaac atctgagtgg cgccaaaacc aagtccggcg agaaaatcag acgtaacata
    11581 ctggatataa tcattcatgt atcgcttact agaaggtaag aattcttcat gtagccccat
    11641 catatcgcca aaaggccgga ctagttttac attggtatca tcataaaaat ctagcacttc
    11701 atcacaccgg cgctttagtt catctactga ttcggcactt actcgaacca catagctcag
    11761 tttgtacatg gattctttgg actgatccaa ggtagtttcc aattcatcta cggaatccaa
    11821 tgcatctaag acattggaat ttgtttcatt gtcagattga taggcatggt tgtccaagtc
    11881 ttttaattcc tttttcttat tgcgaacggt agcaagtgcc tttttgttcg tcacaatctc
    11941 cacattcata ctagtgtcaa ttgggaatgt aaattgttgt tgctggtaat agaaaagttc
    12001 tgaggaggga aactccattt cccccacaat tgtattaatt gttaagtacg ctacatatga
    12061 ttcatgattc tcatgttcca tgcataagta ccggggcttt tcttcaatca aacaacgact
    12121 gggacgtaat aagtcgtaac gtttcaccaa agtttccgac ttcagctttt tctttggtag
    12181 ctgaaattca tattcttcaa atggtgtacc cttttcgcca tagatatgtt caatcaaata
    12241 catcagatca ctcggtgtga ctcgtctgac tttaaatctt cgagccaatt tactttccat
    12301 tagtttttca agcttggtat atcggcgaat ttcttcattg gataaggaaa caaaatcgcc
    12361 catcaaatga tgattcactc catagacaaa ttcttgaaag ccggagaaga aagatttctt
    12421 gagattcttt agattgacct cttcatctgt cgcaatcagt ttgaagccaa taaaaaagcg
    12481 ataatcaatc tgactatcgc caatcatgga tactaatgcc tctgtctgta aatcaattcg
    12541 ttgtttcgct acttctgtta atcgcccggt aatttccttt tttgatcgtt cttgcgtggc
    12601 acgaatacta ctttctgtgg caatttgtaa ggcatgaatc ttgccttcac gattttgcgc
    12661 aatcaattga cgaaagttat cgtggacttg atatttctgt tcaggtgaca aaaaggagta
    12721 attgtaaggt attaactcat agtaggcaaa acattctccc tcttgattaa agacaagatt
    12781 attttcaata tacttaatcg ggtacatagt tcacactcct taccagagta atgggttcgt
    12841 cccatcgttt cttctgttct ttgactttct ttcctgcgaa agtgacctta aatcgtaagt
    12901 gatagctaat aaaagatcgt aaaaatccca atggtttctt accgtcaaac gttttttgcg
    12961 acatgaacca agtaactgcc accggtatac ctaagtattt taaaaaggcc ccctctatca
    13021 tagatagtgg aggcacatgg gcaaatacga tgacaaacag caaggataaa acaaaccaag
    13081 ccatctggtt aaaagtcaca ggaaaaggca gttgaaagtc attaattgca taaatgacct
    13141 tttcaactgc ccaaatactg gtgtagcttt ttattttctt catcaaatca ttcctttaaa
    13201 aagaaggata gtttagaaat caactaccct tcaataattt tgttaatatg gatactcaaa
    13261 aacacctcga ctcgttacca aataattgcc tccaatctcc aagtctctac cacacgcatc
    13321 aaaatcaata tacgtcttca agtgttctgg tacttctcca aaatcaccag cttctacaat
    13381 caagaaatat gctacatcac tcatatcatc gcaatctgga taatagataa tatcatccac
    13441 atgctctacc atctcctcaa aagaattgaa gaaagcatgt tgaatctcag aagcaacttc
    13501 accaactggc gttccttcaa gttcctccgc taagccacac aaccgattaa tttcctcaat
    13561 tggcgtatac tcatcaatct caaagggaag ttcataatca tgaattgcat attcctcgta
    13621 ttcgtcattt aacccaattc gttctttcac ttcatcaaaa tcgacaggtg gggtaaacca
    13681 cgcccccacc aattcacctt cgttatattt cccgaggttc gcaatataaa cacgcatctg
    13741 ttccatcgtg cttccccctt acgcaccgac aattcggtta aacaaactga gtaagacatc
    13801 cttcacccca gaagcattaa agaccaatcc gaccgcaatc aaagcaatca ccaaaaagcc
    13861 aatcagtttg gaaaactccc gcttaaatcc taaaaacaaa ccaatgacca caattgccag
    13921 taagactaag gattgggcat tggttaaaaa ccattgatat atggataggt aagttcctga
    13981 tattataaat atctcaaact tttcccccca ttcacacgga gcgtgcgact ttcaccgcac
    14041 tccgcgttcc atcgtgttta tattgaaaag tatttatgta gtagacctat atcaagttta
    14101 attcttgatt gccagctaat actctatatt tatttagtct tatgagctgg actttattta
    14161 gcttcaatag agctatatat ttttgtattg tttcttctgt tttagcgtgt attagacggt
    14221 gtaccgcctc ttttaccaaa ataagatttt tataatcgtc atttccacca gctccagttg
    14281 gtacaatatg atgacaatgt atttcattag cttgttgaaa ttcttccaga gttatagcac
    14341 attttccatg ttgagcagaa aatagcgaaa ttcgattgtc tgcgtattct attgtgcgat
    14401 tttccaaaga taatttcatt aactggtaca tcagtaattt atcaacactt agattggtgt
    14461 ggataaattt tcttccctct agagtgtagc aattcgctaa atacaccttt gccattggtt
    14521 ttctgtgttg gatatatcca acagggaaaa taggttcgcc ctcgccagca acataccgaa
    14581 gcattttaga tttaccatat cttgttgcct cctttgtcgt aagttgtctg cctgtttttc
    14641 tcaatctgcc ccttttgttt gtacctaatc tgttagtgaa gatagccatt acagctctat
    14701 tcagtttcat acaatccaga tttacattcg ttgcaatctg ataataattt tgcataccta
    14761 ataccattga attataaagt cttgtttctt ctaattccgt tttccctttt gagggagaag
    14821 caattttctt tgcctgtgat accagcttgt ctttttggtt tttgaattgt ttatcactta
    14881 tatgtgattt aacaactaac ttctttcttc tcggtatcat tttaatcttg aaaccaagaa
    14941 agtcggagta tctttttcta acatttacaa ttcttgtttt ttcttgtgat acttccaatt
    15001 ttaatcgttg ttcaatccat tgagtgattg ctattttggc tttttcagca ctttccttgt
    15061 agcggcagaa aactctaaaa tcatctgcat aacgaaccat atacatttcc tttaactttg
    15121 ttttcttcat ttctttgtaa gcgttgcttt tacatgggct accgctttta ttgatatgaa
    15181 ccacatagtt ttttgtaaca ggattttcct gccattggct ttctacccaa tggtctaact
    15241 cattaagaac aatgtttgct agtagtgggg atattattcc cccttgcgga gttcctttct
    15301 ctggatatgt tattgtaccg ttagtcattt tgattggagc ttttaatatc ctttttaaaa
    15361 tgaatatcaa gtgcttgtcc cgtattccca tagcccatat ctgttttatc agcttggaat
    15421 ggttcacgtt gtcaaagaaa cccttaatat caaattcaat cacataatgt agctggctga
    15481 tttgtaggcg gttataggtt gcttttattg cattttcaac tgacctatta ggtctaaaac
    15541 catagctgtt ttcactaaat ttagcttcgc agattggttc taatacttgt tttatacatt
    15601 gttgtataag cctgtcccac atacaaggaa tacctagagg tctggtttta ccatttggtt
    15661 ttggtatttc ttttcgcctc actggcttag gtcgataccc atgtggactg cctgcaacaa
    15721 tgtatcttac ttttttgaca actgtttctg ctggcaattt gccaatatct ttaattattg
    15781 taccgtctgt tcctgctgtt ttactgcctg catttgcttt gatatttcga tatgccagta
    15841 atatattttc tctcgatagg ataatatcca taagatgagt gaaaatatct ccattcttac
    15901 ttttggcata taaatcatca aaggtgtttt gcatttcgta atattcagcg tgtcttaaat
    15961 cttctacaca caatgtttca tttttatttt taggcataag gcttcactcc tttacttgaa
    16021 attgagtgag cttttttatc ttactcgtaa tccttattga aacaacataa tacttttcta
    16081 atatatacac gacttgaggg cattcctcca ttctcattac aagaatttct taggttcgcc
    16141 ctctgctttt cagcattagt tcaactgctt attgttcttc gtcagtttat tctcttgatg
    16201 agaacccaat acctttcctc gttccgataa tcttatctat acatatatat ccttaggtca
    16261 ctagctataa gcctgctggc ttgataccgc ctgtaacgtt atatggcatt tcataaccta
    16321 tattcttact ttgccacacc agctacgatt tctcgtacct ttgcatttct acaaagttca
    16381 aatttagacc cgtacattcg ctgtttcgtc agttccttat agaacattct aaccatagat
    16441 attttataga cccccgacct gttggatacg ctacccacct ctggatagtt ttcgatagaa
    16501 gatatatgtt catcaactat tacggtatct ttcagccgtc tttaccgagc ttcaaacatt
    16561 ctgagtttcc tcatacaatg cttgtcggag gctcagggca ggcgtttcag agcgttactt
    16621 catcattcca cctgtcagat tatcagttct cctaataacc aaactttttc gttcttttat
    16681 tagtgcctta gcttttcact aaggaacgag tcgcacataa attttgtccg aaattcatct
    16741 tgtcactttc ctttcttttc cattgctcga tccgctgctg cacttgcatt catcaagcac
    16801 atcacagtaa caccaagcat actgccacca aataccaaaa aaatatcttt tacaattccc
    16861 atgacgattc ctcttttcat ataatcaaat tttcaattgc catactttgt tgctccaaaa
    16921 tctttttatg tcggtctgat aagcgtgcat tctgtaccat atcctggata taggttgtcc
    16981 gattcaatcg atccaactcc ttagccattt tccacgtagg ggcgacttgt cggcctagcc
    17041 aattcaaggt tcgttcaatg gtatacggtt ttggctcgga agttaattga atctctttcc
    17101 gattccgtcc aataaaacgc ccccattgtt cattcaaggg ccagttggtt cgccttttac
    17161 cttctacttt atccgcaaat cgcaagtaac gattgataat ggaaaacgtg gtgctctcaa
    17221 tgctacgacc ttttaacaaa tcttgaatcg catgaaaagc acggtcattt ttcagtcgaa
    17281 tctcaaaacg atttttcgtc tcagtttggt ctaatggaat ccctaacttg atatattgtt
    17341 catagtcttt ttcatacaaa cagaaataaa cctcactctt gagactgccg atatataagg
    17401 tattgcccat gccgtccttt tcatcagcct ttaaaagttc cccggaacga taacttttaa
    17461 aggtacgaaa caaggagata cattcttcct tctgacattt tttcgttagc tcaggtatat
    17521 ccaatagccc cactcgatca ttgattgcca agtccaagcg tttcatcacg ccaccggcct
    17581 ttagacaatc ctcgaaaaag tcgtaccagc tacgcttttg agcgagtaaa aaagtttcaa
    17641 attgtcggca acctcgcccc tttaattcta aaagcacacc tttatcttct tcatgagaca
    17701 acatcaccac aatatcaccc atgacatact gttcttgata ggagtaaaag gcataatctt
    17761 catggagcat atactcaaat ttcaaatgga gaatttcttg aataattttt cgtgcatccg
    17821 ttgtgggaaa gcgaatcctt acatagtcaa acagtaacgt caaaggcaaa tcgggattaa
    17881 agctttccag ttggtcaaaa attttcctca tggtttttat agatgcggtt acgccaccat
    17941 tttctatctg atttaagtac ggccgactaa gtccacaagc cgtagccaat ttattttgtg
    18001 aaacgccgta ttcgttgcgt cggtctttta atagccgata atctaaattc cgttgtgcca
    18061 agttctcacc tccaaattca aggtgttaac tttttcaaat gagttaacac ctatacaaat
    18121 tacttactat ctttgataca acaagcattc ttaatggttg cttacttatt ttttgtgttt
    18181 ttgtaccccc ctgttagata cgggggtgag acatcatggt cggctaacgc cgtccatgaa
    18241 caggcacagc accacggagg atttctgctt cgcgaatcct ccgcggtcag cgcctaacag
    18301 tcagttgtgt tggtttaggt tactcatctg taataccatt tacttccaac tctgctttca
    18361 tttctaacaa gtattgacga atatctggtt cttttgcttg gtccgcaatc cgctcaaatt
    18421 ctgcaattac ttgatttatt tcaattcgag catccgtctc ctcattcatt aaatatggat
    18481 cgccagcgac tttgacacca ttcttgcgaa tatcaaattt taaccgtcgc aattgttctt
    18541 ctaattctgg gtctgactga atctttccaa tcacccaata aatttctcca atcaactcat
    18601 cataaaccat gtacatccaa ctcttttaat cttctggcaa tatcaaaaat gaccggaact
    18661 gttacagagt ttccggcttg cttatacaac tgactatctg aattgacttg agaatctcga
    18721 tcaaaagccc aatcgggaaa gccctgaagt cgccaacact ctctaggtgt tagcttccga
    18781 atttgatact gatccgtgac gatcgcttgt tggtctcctg ttagtaaggt atgaacactc
    18841 tgttttccca gacgacctcg gcgacttgtg gaattaggaa aagccagatt cacactgtct
    18901 tctggcttca cctcttgata ttctgtttca ttttttcgaa tcatgatttt tgtaccacct
    18961 tgtttcgttg acaaggtggg ggcggttcca gtaacttcat aaactcgatt accttgttca
    19021 aactttcccg gtaatagtcc tgctacagag attccatccc ttgctggcag tgcgattttg
    19081 attccttcat cacctgttaa ggttggcgct acgccatctg aaccatagac ttgtccaccc
    19141 attcctcgat tggaaggatt gatatttacc aaatttctga tgttttgatc agatgttgaa
    19201 gacggtccaa tgataggaaa tacttgttcg gtacatgcgt ctcgagaatg tccgataagg
    19261 aatatccgct cccggctttg gggaatgtag gcctttgagt taagcaccta ccactccacg
    19321 tcatacccca atccatccag cgttcggagg atcgtctcga acgtagcccc tccttcgtga
    19381 ttgagcaatc ctcggacgtt ctcaaggaat aaaagcttag gtcggagaag agtggtgaac
    19441 cgagcgattt caaagaataa agtacctcga gtatcggaaa atctttgccg ctttcccgca
    19501 attgaaaaag cttggcacgg aaatccgccg caaaggatgt ccactggtcc gagggattga
    19561 ataaactcat ctgatacact tgtgatgtca tgcatttcca cctccttact tgtatcatga
    19621 attgctttat aactacgtct agcaaattta tcaatttcac aaaaaccgat acaatgatgg
    19681 ctcgcctgtt ccatacctaa acgaaagcca ccaataccgg cgaataaatc taaaaatctc
    19741 atacattacc tcattcattg caaagaaaaa agccaagaac attttgctct tggctcatac
    19801 attattaatt tgtcctacta ttttttattt aatccaacta atttccgttg tttttcattg
    19861 gcctgatcgg ctacatattt aatcaccgtt ttgaactctt ttgcagtagc attttccgat
    19921 aaatccgcaa tgctcttatt gtatttcttt acaattttcg ttacttgttc tttctcagtc
    19981 atgtaattga acctccaaat ctttcctact ataccctagt ggtaaaaata attctagatt
    20041 atccgtagca taatgaactt cggaaatatt attcgtataa tcttttatta tgatatgaag
    20101 caatacatct ggatgtttac tctttacttt tgcaatgttc tctcttgatt tgaaaaaagc
    20161 gttaataaat gcctttttcg gcacatgacg tccctctgca atttcacgtt cttgtgtaaa
    20221 tttccaagcg attttagggt cttgataaac atagaaaatc gtcactcgaa aatgtttttt
    20281 caacgcccgc gagatatttt tttcagcact taatattgca aaagttgcat ccagaataaa
    20341 agaatagcca tgatctaaga catatctaaa tgcctgctct accaaccaag aagatgcttt
    20401 ttgaaagaga ctagagtttt ttccattata atcaggaaac tgggtgcgaa agtaatccgc
    20461 atcaataatc acatggttat cataattctc agctaatcca atggcaacct ctgattttcc
    20521 agccccagga ctccctgcca taaaaactgc gtctcgtcca acttcggggg atttcccttt
    20581 gataatataa gaaataaact tatccttatt tgcttttgca tactctaaat aagaatcatt
    20641 actttccata ttaattatcc cctttcttcc tactatataa aagtattata cgttacttga
    20701 agtataaagt cagtattgaa gatcactcaa tcattcatct tttctaattt actaattgaa
    20761 tctaagaaat catacccttt tggtacaagt ggcgtataaa actcactaat cacactgcca
    20821 cctgtatcca cataacctcg acctttgatg tgcttcataa agaagttttt ttcgacctcc
    20881 ccaaacatca tggaatatcc gagttcactc atacgaccca aagccacgcg aaagttgaat
    20941 tgatcgcgga ttccgtctcc taaatatttc gcatccggcc tttggcaagc caaaatcaga
    21001 aagaaaccag attgacgacc caacatcacg atttgtttga gcttattcaa aatcaccgca
    21061 ctttctttcg tcgttaacat ctccatataa gccacgtatt catcaaagat taaaaagttg
    21121 ggtggaagtc caagataggc ataattctct cctgttttgt agttagacat ttctttcatt
    21181 gccttgctac gagccatcat acgttcatag aaatcttcca cacaagcaga aatttcttcc
    21241 ttttgtgaat aaacatgagg catcaccgta cctaaatcag ctaaatctgc atttttggga
    21301 tcgaggataa acaattctgc atctgacttc aatagtgctt cgataatggt gaggaggaaa
    21361 taggtcttcc ctccacccgt gccaccagcg attaacatat ggggtaaaga atcataagcc
    21421 catacttgat ttttcatcaa tcgcagagtc ccattttctg ccactacttc gtctatccca
    21481 attcgattgg cgatcatatc atacagtaaa gtatactcca cataggagtc tttgagttcc
    21541 ttttctacta actcacaata cagtccgctt tctaacttct tttccaattt taaaagctga
    21601 tcttggtatt tcccaagtga aatttgaacg cgaatagaca gtaatccatc tttcattcga
    21661 taacagattt tggggaagta actgatagtc tctctggttc ggctactatt caaatctttg
    21721 aaaaagcctt cactttgagt ttgtttcact tcataccaat ggttctctag aatcatccga
    21781 gctagctttt gtcgatgcca caagcttcgc caatgatcaa gaaaaaagtg aatataaagc
    21841 aatatcatag ctcctgaaaa agcgagactt ccaagcagac atactaaatc ttttatagac
    21901 caaacgattt caatagtaga aagtgatagc ttagaaatat caaattgtct aaactcttga
    21961 aaataaaaga cgctcatcca accgatgaac agagcgagta gccagccaag aacaaagtga
    22021 taaaccaagt gctgatctcc tgcgcgtatg cgatgtcctt tgtacatttt gatggtttcc
    22081 acctccaatc gaaaaagccc aataggattt cattaggcaa tttcaaaaag ttatgctagt
    22141 catttactgc tacgaggagg ggagttgttt cgtttcaacc aattgattta aaaagccttc
    22201 aatagcatcc ggcatctcca caccaatttc ttccaaaatg gattctgcct gccgacataa
    22261 gttttcattt acttcgacaa ctataaagaa agactcttct tctcctaacc agtctagtac
    22321 atcagagtct tcttcactgt cgctattttc tccaagtaaa ccttgatgta taatcagaaa
    22381 cattttttca tcagaggtta atgctttaac gagttgttcc tcagtcagct cgccttttcc
    22441 atattgatcg agacaatcta aaatatgttc aacattaatt tccataatct tcacctattt
    22501 ctttctttca ttttggttcg taggatttcc ttgattcggc tgccgcttca agactaaatc
    22561 atccgcctta atgaaccaat taacggtagt tccttctcga aaaacataat tggcaacggt
    22621 atccacgatt ggattgacca actctacctc tgcatcataa gcaaactcgc gtaagggtac
    22681 attagctggg atactaactt gaatcattcg cccttgtgca gaggatttta aattataggt
    22741 tcgttctttt acctcattac caattgttcc atcctcattt tgcttccgta cttctcggcg
    22801 taatgcggaa aaacgcaact tcccaaatgt taaacctgaa tctacgacaa taccttctgt
    22861 taatctcatc ttcttttcct cctatgcttt cactaaatca tcaacataca acacgtaatc
    22921 agtaaaccca cgattctcaa tctcatagcc tttggcaacc aaatggggat tcacggcttt
    22981 tagcggttgg tcttgatcaa aatccttttc accggcttcc gcagctacta ccacctcaat
    23041 atcatcggcc cgttgcttac tggaatacaa atgataactg cgaccaatca ccgtagtatt
    23101 ccgtccatat ccttctgtta taatttctcc ttcaccggca taacttaact ttccaaaggt
    23161 ttcagccata tctggcacga caaatttcaa ttccatcttc ttttcctctt ttcattgtta
    23221 gttttggata caaaaatagc gcgactactt ttttaggagg cggtcgcgca cgttggttct
    23281 taggatttac ttgtatcatc tcatcacctt agttccggcg attccagaaa taatagcccg
    23341 ccgtcgcaaa aatcaaagta aaaccaatga acagtaaaac attggaattt ttctccccgg
    23401 tttgagggaa ggtcttcact gtgggactag attcttgaga gccacttgga acagttggtg
    23461 tttccggttg tttcggcgta cttagtatgg ttggaacttc ttttggtgtt aaggtttgag
    23521 atttttcttt caaatcttca ttgtgttttc cattgatatt cccatcttta tcgtaattga
    23581 tttccgcaaa agtaaacttg gttccttctg gatattttcc ggtatctact tttttcgcaa
    23641 gcacagtttt ggtgaattct ttatcattta cttcataatc aatcttgcca gatttccaaa
    23701 tttctttgtt tgtaccatcc ggtaataaag catacagaat tgtttcgaaa gcttcttttg
    23761 agccatccag tacatcatga gtaatcgaca catcatcaaa catatccacc acatcaccat
    23821 gagtaaaggt ctgagaaccg ttttctaggt gggcctttgt ttggatagaa acatggtgtt
    23881 ccactgtaca actgacagtt tgggcttggt tattcaagct aacatccttc gctactggct
    23941 tatcgcctgc ttcaaaagct tccttatttt catacacata gttgaataag acaatagttt
    24001 tatttaaaac ttgttcggcg gttaatttat gctgaatttt ccaagttcct gttttctctt
    24061 tgttcgccaa cgttgcgctt gtttctgcaa tcaccactgg tttggctttt tcatcttttt
    24121 cttgggcagc ttttgtcgca tccacatcaa tggcttgcgc taccacatac cagtcttctt
    24181 tgatttcatg tagattgtaa ctcaatttat caactagctc ggtagaatcc agagaagtta
    24241 attctttgtt tccgtctttc caagtcgcaa gagaagttaa actatcttct tcctcaggca
    24301 aatcatagag cattaaacgg ttcaaactaa cagaaaatgc tttgttcgtc agcttctcgt
    24361 aaggaacggt caccgtttta attggttgtt tttggtcttg ttcggtaatg gtaaagacat
    24421 actcactctt cacaaagtct tctttgtttt ccttaaaagt agaacggatt tctaatggcg
    24481 taatcttttg aaaaccttct ggggcttcta cttcttcaag taaataatcc ccataaggaa
    24541 gattttcaaa cttgccataa ccatcaaacc ctaattgctc gttataagct gtggtcgctt
    24601 catcttcggc acctgtgatt tcattggtcc cttccaatgg cgacacttta aaggtcagat
    24661 cattgaagcc agtttcggta gtgcccgcag ctgatccagc aaatttaaag aaatcaaagc
    24721 caaagcgaat cacttgttct tttgccgtaa catttcgggt aatcacggca tttttttcgc
    24781 tatcatccac ctttttgatg gatacaggat actttgtttc atcaaggcta tatccttcgg
    24841 gcgcttttgt ttcttgccaa taatactcgt tgatagctaa atgtttaacg gcaacttggt
    24901 tcttttcgtc taaagtcaaa gtgaccgttt catcagaacc tttcgttccc ttcgctaact
    24961 ctggtttaaa agcctcactc catttaacag cttttccatc cttagcagta aacaaggtgt
    25021 attcagcacc ctcaaagact gccttacctt gtgctttatc cccagtatct ttgtcttctt
    25081 tcgtcaaagt ggtttcgcca gtgacttctt gattttgccc ttttacgtta ctggtaacag
    25141 gggctacggt ttgattggca tacttcaatt cgacttttac aggtttgaac gtattcacga
    25201 acccatgact agctttagtt tcggtcacat aataggttcc caattccaac gcattagcaa
    25261 tctcttttgg tgtttccgca tgaccgtttt catccgtggt catttcttga acgatttcac
    25321 cggttggact gtctttgcga atggcaaacg tattaccagc taaggaataa ttgtcattcc
    25381 aaagatcact ccctgtttca actccgcttt tatccagaat gatttgccct ttttcccgtg
    25441 cgttttttga agtcacgtaa atggtttcgc ctgctttaat ggtggtagtc atgggggtgg
    25501 tatcaatcgt ataaggcgcc ggtactgatt tttcggtaat ggttactttt gtaccatgag
    25561 gaatctcatc caatgtagca atgccttctt tgtcagtagt cacgtctttt gcaggtaaag
    25621 tttttccaaa gtctaaatgg aaaaccgttc ctggtacaac atcacccgat tctttatcga
    25681 cttttttgat ctttaaagaa ccctccgttt ccacatcaat tttgattgtg taggtattag
    25741 gtttatcaat cgccccggcc attaaagtct gttggccagc ctttttatag gcgacaggtg
    25801 ttccggtacc agcagatttc ttaagcgtca gcacaccact ttcttttgaa ttggcatttg
    25861 gggtgattat caactgatta ccattcacac gataatcaat gtttgcggca ttttccacaa
    25921 cctcatcgaa ctctgacaaa ttcagattat tcgtatccgt cacagtagtc gattgcccta
    25981 atactgtttt agccgtacta ttatggaagg ttggtttctt ctgataatcg tcaatggctt
    26041 gatttatttt tgcctcaatg gctgcgatat ttacggcact accatctgat cgttttattg
    26101 agtgaagggt atacccatta acttcttgcc agatcatctt ctgtgccaca atatgagtat
    26161 ctgcatctgt acctactttt ttccataaaa cggaaactaa tttggcttta tcggacatat
    26221 ctggtaacgg gtttttctca tagccaggag tgaactcatt gtaaatcggg actcctggtt
    26281 caatacagaa aatatcttgt ttaaagccgt tgtacactgc ataatgcgga acagtgactt
    26341 ctgacattgg acgaccatca ctaaaagttc cttcaacagc atattgatgt gctaaatcta
    26401 gttctacggt taccgtttgt ggatgggtaa tttcatcagc aaaagtaatc gtaggagcaa
    26461 ttgctccaac aattgcttga acagccaaac tcaaaatagt taatacacat aatttctttt
    26521 tttgtttttt atacatgaaa tgttctcctt tcaaattaag cacaaaaaaa taagatgctc
    26581 gcttgaacat ctttgtttat aaagtctttt taaaattttt attgactgaa tagaatggat
    26641 ttgagaatga atgagcctaa aaatttaatc taaaatacga aatatactgc ctttaaatta
    26701 gaacacattt tagaacacaa acatttcaaa tttaatcaaa tataataaaa tatatttacg
    26761 ggaaaacctt tataaatcaa ggtttttcaa acttattcaa tgatacgaag aaaaaaccag
    26821 gtcttaagaa agcccgtaaa gcttcacagt tctcaaaacg ttaattgcga ttatcgttcg
    26881 agttcacgag ttacgagaat cgtactgggc gaagcaatgc tgagcgcagt taccttaatc
    26941 ctaacttttc tggcactttc cacaatttgg aaagtgcctt ttttgtgttt ttagctatgt
    27001 taataggtag gattcttggt ctataatatc attcgatcta taaacaaacg ggaaaaagat
    27061 aaagtatcat caaacaatat tatttatccg cttctgaggt tgtgacagaa gtggtcagct
    27121 tcaagaaata agaaggaatt tacaaaaatt gcttctcatg tttgataaat attgtgactt
    27181 gtgaccgagg agttggcagg agaacaccgt ttattcggaa gaagggattg tgaaaagtct
    27241 tttgtcataa cctcttattt ggtagctaat tgtaaatccc aaacggcatg tattattttt
    27301 cataagtccg tttttaagga ttaatgtttt cttttgcttt ttatatggtc tggcttcagc
    27361 gagaagttcc aacgcatata caaggacgac ttatgtcaac gataacaacg attaccatgg
    27421 tggccagtcc acttggatta gcactgtttg gtgcgctctt tgattggaag acaacgcaac
    27481 cacgtctgcg agacagtcta atttttctgg gagggagtct tgctatagtt atccttataa
    27541 ccggtctggt aaaattactc aagcttgatc tgaaagaagc aaaaattttc agtgaagagg
    27601 aatacagaga aacgactttg gtcgaaaaga aagaatcagg aaactgatac aagcgtataa
    27661 caaactgcct gataatagga aagacacgta ttgtccgatt gttttttttg acattcaatt
    27721 ggaaaattta cactttcttt atacatttct ggctgctttc tttatacccg tcaactatac
    27781 ttcactcatc aataagaaag aaggataagt atggattttg gaaatgaaac ggtcttgaaa
    27841 gcaactggta taaccaaaaa atacggtgct gccaaagcat tagataaagt atcaatcgaa
    27901 atcaaacgtg gaatgatcta tggattgatt ggggaaaatg gagcgggtaa gtctaccttt
    27961 atgagaacta ttatgggttt gatctcgatt gatgaaggtt cgatcgaatt atttggaacg
    28021 acggatttgc aggcagcaag acgacgtatg ggtcaatcga ttgaaacccc tgctctttat
    28081 ccagaattaa ctgcaagaga taacctacgt atccaagcag cgaacggcgg tgtgtcggat
    28141 agagaaatcg aagacctgct gaaaatgatg cgattggaaa atacagggaa aaagaaagca
    28201 aagaatttct ctttaggtat gcgccagcgt ttagctattg caaatgcact gatcacgaat
    28261 ccagaatttt tgattttgga tgaaccgacg aatgggatgg atccagcagg gatggcagaa
    28321 atgcgagaaa tcatccaacg tttggtaaaa gaacggggca tcaccgtctt gctttctagc
    28381 cacttgctag atgaattgtc acagatcgcc actcactatg gtattttgca tgaaggacac
    28441 ttgatcaaag aattatcgaa agaagaattg gcacaagaat ctcgtcaatt tataaaaatt
    28501 gacacttcgg cgacggaaca agcagtaacg gttctcgact ctcttggtta tagggattat
    28561 tttgtacaat ctagccgtgt tatccagctt tttgaaggaa ttgaccaagt agcagccatc
    28621 aatcaagcat tggtagaagc aaaagttcct gtggatggta ttcacctagt tgggcaaaaa
    28681 ctggaagatt atttcttaca attaactgga ggcaatccac atgtttaatc tattaactgc
    28741 tgaaaaaata aaagtatgga agagccgtaa aatgtgggtc tcactaggac tgatggtcgt
    28801 acttccgctt ttttattctt ggaacgaatg gtatatgcat acaaaatacg gcaatgaatt
    28861 aaaccaagca acggatacag tcatcaacgg tgcaacaggg attttaatgg tagaaaaaat
    28921 ggctgcatgg attcttctgg ctttcgcagc atttgcgtgc ttttatattg gggaagaatt
    28981 tcaaaacggt acgattcgca atacgttatc tttaggacgg aatcgaatga cttattatgt
    29041 gtcgaaactt ctcgttacgc ttttgatcac aacacttggc gtggttttga tcacgggact
    29101 agccatgctt gcttacacac ttgctttcgg atttggtgaa gttgagggaa tcaaaaacta
    29161 tggaaattat gttctaaaag tatttcctgt attgcttctg ctgacattag ggacattatc
    29221 tgttccagtg gctttgacat tcatcactag aagtaccagt gtcagtttgc ttctttcttt
    29281 tctttatatc atgggaacgg ccttcattcc cggtgttttc gcaaaaatca aaggcttgga
    29341 atttctaacg gagtggttta cggaaacgtg gctcatgtat acggactttg cgcaacaagc
    29401 aacttattcg caagcaccga aaatgattct ggtcagtcta gtaacaatcg tggtatcatc
    29461 agctctagga atgtttattt tccaaaaatc tgacattaag taggtggtcc tgctggaaac
    29521 tattttgatt atcgaagacg atgaagctgt tcattcctta ctggaggagg ttcttgaaca
    29581 gagatacaaa cttcttgatg cttattcagg tacggaagga aaattattgt taagcacata
    29641 tcctgtcgat ttgatccttc tagatctcat gcttccaggg ctttcaggtg aagcattgtt
    29701 agcagaaatc cgacagacat cgaatgttcc gatcatcgtt ctttcagcaa aaagcgatca
    29761 aagagataaa gtttccttgt tggcagcagg agcggatgac tatgtgacga aaccatttga
    29821 tatcgaagaa cttttgttac gaatcggcat ccaacttcgt catcagactt ctgtccaagt
    29881 aaaagatctg tcgcaacgca tctattacaa ggaaatctta ctggacaaag agtcgaggga
    29941 tgtaaatgtg aatggtgtgg ctgtccattt gacaggaaga gaattcgatc tgctgaagct
    30001 ttttttagaa catccgaaaa aagtattttc gcgagcaaat ctttatgaaa cagtttggca
    30061 agaaccatat tttgatagtg agaaaacagt gaatatgcat atttccaatc ttcgagggaa
    30121 gctagcaaag gcacatgctg tgtatattca tacggtgtgg ggagtggggt ttagatttga
    30181 ttaaatggct gttgcttctc gttgttatct ttatgggaag ttttattttc ttcctgcaaa
    30241 gggaaatcaa gagattaact aagaaaatcg ccactctttc tgaacgacaa gaatatggtg
    30301 gtcgtctttc agtcgaattt cgagaaaaga atctaatgga cctagtagat gcactgaata
    30361 catggttgga tgaaaatgaa caaaaaggac aagcatttca agaaatggaa gaaaacatca
    30421 gactatcgat cgtcggcctt tctcatgatc tcagaactcc tttgactgct attaacggtt
    30481 atgtacaatt actggaacag gtagaggata cggagaaacg ggagaagtat ttaacgatca
    30541 tcaaacaatc tgttgcaaga ttactcagta tgacggatca gttttatgat cttgctcgaa
    30601 tagagacgaa gcaaaaagaa atggatctcc atacgctttc tttttctaaa gaggtggaag
    30661 aagtattttt ctctttttat gaacaatttt ctgagaaaaa tatcgaggta gcgttcgctg
    30721 aacactcgat aaatacaact gtattagcag atccgctcat gttgctgcga gtgctgcaaa
    30781 atatcataca gaatttattg cgttatgcag atggacaagc aaaggtgaca ttttcaaaag
    30841 aggatccatt cattcgatta acagtggcca atcctatcaa accaggaacg caaacctctg
    30901 ttgaaaaagt cttccagcgt ttttataccg aaaataccag ccgcacaaat acggaatcta
    30961 gcggaatcgg gctttacctg tcaaaaaaat tggtggaagg aatgggagga gagatgaccg
    31021 ctagattaga cgggggcatc ttctccatct ccgtcaagtt gcggagggtt taattataca
    31081 agattagtga gaactagtag aaaaatgtat tatcttatat atatttaata gagaaaaaag
    31141 cctgtgatat aaatattgca ggctttttta ttgatttgaa ataatcaaga atattagaaa
    31201 cttctacatt aatcaagaac tttcttctgt atgaaaatag agataataat tgtacactta
    31261 agctgtattc aggaggtgaa gataatgggc atcgaattta gcgaacggca aatgaaaata
    31321 ctatactttt tgattgaaaa tgagcacaca acttacaaag aattatcaga acaattatat
    31381 gtatctacga aaacaattag caaagatatt ttaattttga aagaaataat ccaaaaaatg
    31441 ccagatgtac aaatgttggt tcaacctaaa aaaggcgttt cgcttattgg aaaaaaagaa
    31501 acacttcgaa atttattatc cagtcataaa atcaatatcg cagactctcc ggaggaccgt
    31561 ctaacttatg tgtttgctaa attattgaat actaatgatt ctctaaagat tcaaaatctt
    31621 gctgacgaat tatacgtgag caggtcgacg gtagaaagca caatgaaaga tgttagaaaa
    31681 agactaaaca agcatgggat gtccttagtt tcagatcgaa atggcgtgcg catcaaagct
    31741 gatgaaaaaa gtaaaagaag gttgatgtca gaagttgtga actattattg gggtggcttt
    31801 acagcatcta acagtaaaac agatctctta gacttgaata tcaatttcat tggtgaagcg
    31861 aataatttct taaataagga catattgaaa aaagtagccg aattgcttaa tcgatacatg
    31921 aataagacac aattgaaatt tacagattat gaatttcaaa ctttagctat ccaccttgct
    31981 attgcgatag aaaggattga aacaagcaat ttcttatcac gtagtaataa ttctgaagaa
    32041 gaaatctcaa agaatacact tattttaatc aaaatgattg aaaaagcatt ttcaattatt
    32101 gtgccaatag atgaacaagc ttatgttgac attcacatta cttcgattga aaaaaatgct
    32161 atgaatcacg acttagatca aacgaagctt tcctatttag atatgggtgt tgagctacaa
    32221 gagttagtaa gaattaaatt aaatgaattt tatcctgatg aagaattgat caataatcta
    32281 gtattacatc tcaatgcagc agttaaaaga ctcaaattgg gagtaaatat ctataaccct
    32341 tatacggaca aaatcaaata ttcttttaaa aggtcattta tgataagtgt agatttatta
    32401 gaggaaatcg aagagagatt ctgtatacat tttaatgaag atgaaatcgc ttacgtaaca
    32461 ctccatattc aatcactttt ggatcgttac aaacctgaca aaacgaaagt gatattagtt
    32521 tgtagtagcg gatatggaac atcaaaatta ttagagcaaa gaataaataa cggttttgca
    32581 gatatggttg agatagaaga tgtactaagc atcaatgaac tacaagatag aaaagtcact
    32641 gatgaacttg ttataagcac gattccgatt gaaaatacaa attttcctgt tattgttgtt
    32701 agtcctttga tgttaggggc agatgtcaga aaagtaaaac agtatctaca gttaaatgaa
    32761 caaacagaag atgcttttct aaatttatta gataaacgat tttgcttttt tacccaccaa
    32821 ataaaggata atcaagtgaa cgttttaaat ataattacca aaaaattggt aaaagaaaat
    32881 tacgcgaaag aagggatact agagggagca atcaaaagag aaaaactatc ttcaacagcg
    32941 atgaatgatt ttgctatgcc gcatatcgaa gtagatttta taaaaaagcc agtaatctgt
    33001 gtctacgtta atccagaagg aattgattgg gatgggacac ttgttcattc tgttttcttt
    33061 tttgcactta atgaaaaagt aaaacctgaa ttgaataaaa tttatgaaac attcaatgaa
    33121 atcgcttcga atcatgagtt attagcaaat ttaaaaacga gttcgtcgtt tgaaaaatta
    33181 gtttatatat tgaaagaagg cttataaatg atcagtgaaa aaatcaatat aaataatata
    33241 gtgatagatt taaatgcatc tgataaaaaa agtgtagtcg aaaaattatc gaaattgtta
    33301 gagactaatg ggtttgtgtc tgaccacaaa caatttcaaa aagacgtatt tgacagagaa
    33361 aaccatatga ccacaggaat tgggaaaaat atcgccattc ctcatggcaa aagcctagca
    33421 gtaattgaat caacagtggc tgtagccaag ttaaagcggt ctgttgaatg gggcgcatta
    33481 gatgatcaac cagttgatct cgtctttttg ttagcaataa aagacactga taaaggtact
    33541 aatcatttac gtattttagc agaattatct ggaagattga tggatgacga gtttgttaaa
    33601 agaataaaag attcgagaaa taaaaaagaa ttggtaaaag ctctatcatt ttaaaaggag
    33661 gaatttcata tgacaaaaat agtagcagta acagcatgta tcgcagggat tgcccatact
    33721 tatatggcaa aagctaattt agaaaagttt gcgaagaaga aagggttaga tattaaagta
    33781 gaaacacagg gggctatggg gatagaaaac aaactttcaa tttcggatgt ttccgaggca
    33841 gatgttgtca tatttgcagt agatacaaat gtttcagaac gaggtcgctt tgacggtaaa
    33901 ccgattttaa cagtagggac ctcagaggta gtaaaagatg gtgaagagac gattgatgaa
    33961 gcaattgcat taattaacaa ataaaaaata tatcgtgggg gagttactaa tggaattcaa
    34021 gaaacaagga agagaaattc taaatcattt ccaaagtggg gtttcctata tgatcccgct
    34081 tgtcgtagca gcagggttac taacttctat tgcagtcatt tttggtggca caggtgtttg
    34141 ggaccagacg gatacattct ggggagtcct gcgaatgatt ggtcagacgg gattacagtt
    34201 tatcgtaccg atgatttcag cctatatcgc atattcaatt gctgaccgtc ccggtctagc
    34261 accagctttt atcactggga tgatgtgtca aaatcttggt atgggattta ttggaggaat
    34321 ggttgctgga ttagcttgtg gctatttagc taatgcattg aaaaaagtaa aagtaccgat
    34381 gcaagttatt agtctaaaat caattatgct gattcctttt gttactacat tagttgttgg
    34441 aatcatatta tggtatgtac tagctacgcc tattcaagca atgacaacag gactgactaa
    34501 ttggctagaa ggcatgtcag gagcaaatgc tgctttgatg tcggctattt taggaggaat
    34561 gatggcgttt gatatgggag gacctatcaa taaaatcgca tatgcttttg gggttgcttc
    34621 tttttcaagc ggaagctttg gacctagtac agccatgtta ttaggaattg ctattccgcc
    34681 atttggaatg tttttagcaa caattttaaa caaaaaactt tatgatgaat cggaacgaga
    34741 taatgcaaag acagctatga ttatgggcct tgtggggatt actgaaggga cgatcccgtt
    34801 tgccgtgaaa gatcctttac gagtaatacc aagcatcgtg gtgggcactg cagtagcatg
    34861 tggtctaaat ggattttttg gtgttactca agaaacaatg ctttcaacct ttatggcaat
    34921 tccttttgtt acgaatatac ctttatattt agtatcaatt gttattgggg gagtggttac
    34981 agcgttaatg gtcaatgcac taaaatccat caagtataaa aaagaacaac aagtaaattc
    35041 agtagaagaa ggaaagtagg agaatctgat atggtaaatg cacatataat tcaacacacg
    35101 cactgggaca gagaatggta ttttacaaat gaagatgcca tcgtattgtc tgatcaggtc
    35161 tttactgaag ttctagatga attggaaagg aaccctaaag taaacttttg tttagatgga
    35221 caaacttcaa tcgtcgatga gtatttggag atcaatccgc aaaagcttaa ggttatccaa
    35281 cgactgatga aaacaggaca actattcgtg ggaccgtggt atgcacaaac agatgcctta
    35341 ttagtggatg ctgaatcaat tttaagaaac ttaatgattg gaatcagtga tacgatcctt
    35401 aaatatggag atccaatgat ggtagggtat ttgcctgaca cgtttgggtt caatgcgaat
    35461 ttgcctacac ttttgcatca agtagaaatc gataatttca tgtgttggcg aggattaaac
    35521 tttgaaacga tggttccatc tccttatttt atctggaaag gattgggaga taaatttgtt
    35581 tatgccatga attttccatt tggttatatg actggaatga tgaccttaga cgcaattaaa
    35641 gatattcctg aatttgcaaa agaaaagttg gatgaagcag ttgaaatgct tcatatgcaa
    35701 ggaaaaaaca aagatatttt agtaccttcg ggtgtagatc aaaagtcaat gattttaaat
    35761 tttgatcaag tcgtagagaa gttgaataaa tacagtaaat tccacaatat tatttcagat
    35821 tatccttctt atgtagaagt tatcagaaac aaaaaagaat tacctgaata taaaggtgaa
    35881 cttcgtcagc cggtttactc tcgtgttcat cgttctattg gctcagtaag aacaaaaatg
    35941 aaaatagaaa attttaaatt ggaacaaaaa attctaaaac gagttgagcc gctcatgctg
    36001 attgcagaaa gaaatggtat ccatatcagt aaaggcctac ttcgtcggtt atggaaaaag
    36061 attttgcaaa accaggctca tgatagtatt ggtggctgtg tttcagataa tgttgcggaa
    36121 gatattttcc atcgaatcaa agaagctaat gagatagctg acggaatcga aaaccttatt
    36181 gtaaaacgga tggctgatgc attaaagcta aaatcgaatg aagtccttgt tttcaacaca
    36241 acgactgaga attatagtgg atcgaaaatc gtgcatgtag tagctgattc aaagaaaata
    36301 cacttcaaag gtagtcccta taatatcatt gaaaaagaag tttattttcc agaacggaaa
    36361 aatgcttatc ggatgattgc tacaggtttc gaatattttg atgaaccagc ttattatgaa
    36421 cttgacataa aaataaatgt aaatgtgcca gcactaggat acactgttgt tgaatttgag
    36481 aaaaataatg aaaaattaga aacagcaatc gagatagaca aaactgagat aagtaacaat
    36541 aaatataaac tttcttttaa agatgggcaa ttgaatttga tagttggaga tagacaatat
    36601 cttgatttcg ttcatttgat cgattcagca aatgatggcg atacgtatga ttattcacca
    36661 ttagaaggag atacagaact atctctaaaa ttagaaacag ctaaagtata taaagattct
    36721 ttacaagaaa cattagttgt ttacggaaaa gctcaacttc ctaaagattt aaaagatcgt
    36781 ttatctgaaa aacccgaaat ggaagaaata tcttatgaaa tatcgttcag tctgggagaa
    36841 agtcaaatcg ttgaaggcac actcaaaatt cataatactg tttactcgca tcgtatgcgc
    36901 ttagcaatta atgttttaga taaatctgaa aaatcaattg cacaaattca aaacggattt
    36961 attgaaaata cccctgatgt cattcctccg aattggtcag aaactatggt agagaaacct
    37021 gttaatttag aaatattcga taagagtgtc tctgtggcag aaaatgatca ttattttacg
    37081 ttttatgcag atggtttaaa agagtatgaa cgtatagaaa atcaattaat cataacctta
    37141 ttttcaacaa caggggaact tggaaaacct aatcttgctt ggcgacctgg gcgagcttca
    37201 ggagatacga caaatgaagg gcatgtcatg atggaaacac cgctagctca agagataggt
    37261 gagtataaag tcacttttgg atttaatgta gaagaaggaa gatttaatga gttcaaagtg
    37321 gcaaaacagg cagaaaaacg gttagaacaa tctatttctt atcaaaagca gaaactgaat
    37381 gtatttattc acaggttaga taacaagatt tggccattac aaaatgaact tgatgttcct
    37441 cgtaaatttt cattgattga attacctgaa gacttaattg tatcatcgat ttatccttct
    37501 tattttcatg aacaaggatt tgttcttcgc ttagctaatc caacagaaca agaaaaaata
    37561 gtacctgagg ctatcctttc ccttgggaca gttgtcaatg cactggagaa taaacaagaa
    37621 ttaacaacaa ttcctcccta tgattatttg tcaattttga taaatgagag ataaatatgt
    37681 aatatacaag aaaaaaacac cttttatttt gaaaggtgtt tttttaccct cttttcaaat
    37741 agcgcaacag tgctatacta aagctaacaa ataaaggaac ggagagggat atgggtcaaa
    37801 gagaatttat cgttttaatt attattgcag ctgttatttt attagttgct ttagacattt
    37861 tttcacgttt gaaattaaag gaaacagttc gtagcaaatg ggaaaaaatt ccttatcaac
    37921 cgagatttga taaagaagaa agcttgaagg aagcatggct cacggagaag aagttccgtt
    37981 catgggatag cgaaatcgat gatcttactt ggtatgacct ggatatgttc gaagtattcg
    38041 aaggaatcaa cagtacctat tctagtgtag gttcagaagc gttgtatcaa cgtttacgca
    38101 gctttgattt tggtgaagat cagcagttag aaaaactgat tgctttttat caggaaaatc
    38161 ctcaactgag agaaaaaatc caatatcaat tcgctcgttt aggaaaaaaa gatcataatt
    38221 ttgcaaaaca atatctagcg gatggaaaaa gtaaaaagat tggacgcgta ggtttattta
    38281 tttttcttgg cttgcttccg ctggtgggtt tgggtttgct tcttttagga caagtactag
    38341 gaatctattt ggcaatcggt gggttagtat tcaatacggt ctattatcta gtgaagaaac
    38401 aaacactaga aacagagctg aatagcatga gttatttggt ttcaacgatt tcagccgcca
    38461 atcagctagc aaaactgccg acaccacttc aagaagagct gaaacagcaa actaaaccat
    38521 taagagacat ccctaaatta ggtttttcat ttcgtatgaa gagtgggagt gaagcagagt
    38581 tgatttttga atatgtcaac atcatgttca tgctgccatt tatctcctat catttcgttc
    38641 tcactaaatt agaaaagcat tcaactgaag ccatcaagct gtgggaattg ctgggagaat
    38701 tagaagtagc cgctgcaata ttgaattacc gtacttatat gcccatcact tgccgaccag
    38761 agtttactga gggcggtgtg acagcgaaag atatctatca tccattgtta aaagaagcgg
    38821 tagtgaatcc ggtcaactgg cagaagaata cattagtcac gggttccaat gcatcaggaa
    38881 aatctacgta tgtcaaaagt atagccatca gctgtattct agcacagacg atccaaacag
    38941 cgattgcaga acagtttacc ctgcaacctg gacatgtgtt gacttccatg gcaatcgaag
    39001 atgatttatt tgaaggcgac agttattttg tttcagaaat caaatcgatc aaacgtttgt
    39061 tggaccaagt ggcttccaaa gaacgctgct actgcttcgt agatgagatt ttaaaaggga
    39121 caaatacgat cgaacggatt gcttcttccg cttcagtcgt tcggtggctt gcggagtatc
    39181 catctttagc atttgtcgcc acacatgata tcgaattgac agagatttta aagaattatt
    39241 gtgagaatgt ccactttgaa gaacaagtaa cagaaggaaa aggaatcagt tttgatttca
    39301 aattaaaaga aggaccttca aggacccgaa atgcgattgc cttgctgaaa gttttggatt
    39361 acccagagac tttagtagaa accgcaaaaa cggaagccgc gttatttgat gaaaagcggc
    39421 agtggcatgt attgggatag gcgaagtgat cgaaagtatt tattggatca aaaatcttgg
    39481 cttttgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    39541 tctcacgctc cttttgttgc acatatgaat agtaatcctt tatcttcgat ttgttttttc
    39601 tgaaaaggtt gtctgacttc gtccgattga tgtctttcat taggagattt tcctgagccc
    39661 agcaacctga tttttcgctg gaaccaagtt gttttttcat ctgctgtatt ccatgataca
    39721 ataaagaatc atgtctataa ggagatagga aataaatggc aaatgcgctt gaaataaata
    39781 atttgaaaaa ggtttatgga agtggggtcg aagcattgcg aggaatcgat ctttccgtga
    39841 aagaaggaga tttctatgcg ctattaggac caaatggagc tgggaagtct acaacgatcg
    39901 gaatcattac ttctttagtc aataaaacat ctggaaaagt gaatgtcttc ggttatgatc
    39961 tggatacgga tctagtccga gcaaagcaac agattggatt agtcccgcaa gagttcaatt
    40021 tcaatccctt tgaaacggtc cagcaaatcg tcgtgaatca agcaggctac tacggtgtct
    40081 ctcgcaaaga agcgatgaaa cgcagcgaaa aatatttgaa gcagtccaat ctttgggaaa
    40141 agagaaatgt ccgtgcacgt atgctttccg ggggaatgaa gcgaagactg atgattgcta
    40201 gggcattgat gcatgaacca cgtttgctga tcttggatga accaacagca ggagtcgata
    40261 ttgaattgag acgcgagatg tgggagtttc tccgagaatt gaacgaaaat ggtacaacga
    40321 tcattttgac gacgcattat ttagaagaag ctgaaatgct ttgtcgcaac atcgggatca
    40381 tccaatcagg tgaactgatc gagaatacta gtatgaaaga gcttctatcc aaactgcaat
    40441 ttgaaacatt tatctttgat ttagcgccat atgagaccaa acctgtgatt gaaggatacg
    40501 cttgtacatt tgaagatgaa cagacaatcg ctgtcgaagt tgaacgaaat caaggggtga
    40561 acggaatctt cgatcagctg acaagacaag gaatcaaagt tctttctatg aggaataagt
    40621 cgaatcgttt agaagaactg tttttgaaaa tcacagaaga aaataatcat acggaggaga
    40681 aaaatgttta atctttattt tactgcatta aaaagtttag ctatcaagga aacgaaccgc
    40741 tatttgcgta tctgggtcca aacactggtt ccgcctgtga tcacgacatc cttgtacttt
    40801 gttattttcg gaaaaatgat cggtggtcgg atcggtgaca tgggtggttt ttcatatatg
    40861 gaattcatcg tgcctggttt gattatgatg tctgctatca ctagttcgta tgccaacgtt
    40921 tcttcgtcct tcttctctca aaaattccag aaaaacatcg aagaaatctt ggtagcgcct
    40981 gttcctacgc atgtgatcat ttggggcttt gtgattggcg gagtgggtcg cagtattctc
    41041 gtcggctctc tcgtaacgat catttccttg ttctttgtac cacttcatgt gtattcatgg
    41101 ttcatggtca tcattacatt attgatgacg gcaatcttgt tttcgttagc aggactgctc
    41161 aacgggattt ttgctcagtc ttatgacgat gtatcgatcg tgcctacatt tgtcttgcag
    41221 ccgttaactt atctaggtgg cgttttctat gccatctcta tgttgccgcc attttggcaa
    41281 gctgtttcga aagtcaatcc gatcgtttat atgatctccg gtttccgtta cggattcctt
    41341 ggaacaatcg atgtgccagt agcaacatcg atgatcgtac ttgtgctatt catcgttgtt
    41401 ttgtacgccg ttacttggta tttgatcaat aaaggaagag gattgagaag ttagaaaaga
    41461 ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac aaacgggaaa atagttcctc
    41521 atattttgtc gtttgttgag ctaataccga gggtcagctg attgaacacc gtttagtgga
    41581 gaggttgtga aaaagctgtt ctgcatcaag taataagaac agccaaacaa aaatagctcc
    41641 tcctattttt cgtttggttg gacttattac cgcagatgca agcttttgaa caccatttat
    41701 tccggaaaag gaattgtgat aagatttttg tcacagtctt tttttgctca atcagaaaca
    41761 tcttttaagc tgttcacttt gcttcttttt ttgtttccta gtataatgac aatgtttttg
    41821 agaaagattg cgacttcact gcttgataga atggagagta acaaataaat gactactaat
    41881 ttttggaaag agctgccaaa gccttttttt gttttagcac caatggaaga tgtaacagat
    41941 gttgtatttc gccatgtcgt aaagcatgca gcagcaccgg atgtcttctt tactgagttt
    42001 acaaactcag acagtttctg tcatccagat ggaaaagaca gtgtacgagg aagattgacc
    42061 tttacagaag atgaacagcc gatggttgcc catatttggg gagacaagcc agaatttttt
    42121 agagaaatga gcattgctat ggcagaaatg ggcttccaag gaatcgatat caacatgggc
    42181 tgcccagtcc ctaatgtagc agaacgtgga aaaggcagtg gcttgatcct tcgcccagaa
    42241 gtagcggcac aattgattga agcggctaaa gctggcggtt tgcctgtcag cgtcaagacg
    42301 cgtatcggct atacggaaat gtctgagatg gaagaatgga tcagtcacct acttcatcaa
    42361 gatatagcca atctttctgt ccatttgcgt acgcgtaaag agatgagtaa agtagatgca
    42421 cattgggaag tcatccctaa aatcattgct ttacgtgacc aaatcgctcc gcaaacactg
    42481 atcacgatca acggagatat cccagatcgc cagacaggag aagaattggc cgaaaaatat
    42541 ggcgtagatg gcatcatgat tggccggggg attttcaaga atccttatgc atttgaaaaa
    42601 gaaccgaggg aacatacaga aaaagaactg atcggactac ttcgtctgca actagatctg
    42661 caagatcatt attcagaaat catcccgcgt tccatcacag gactacatcg tttctttaaa
    42721 atctatatca aaggatttcc aggagcaaat gacttgcgag ttcagttgat gaatacgaaa
    42781 tctactgatg aagtacgaga gatcttagac acctttgaaa aagaacatcc aacgcttttc
    42841 agtgaacaat gatttcttgc ttgaacaccg tgaattagag gtcgtgaaaa agctgtcctg
    42901 catcaagtaa taagaacagc caaacaaaaa tagctcctca tattttgtcg ttttttgagc
    42961 gaataccgag ggtcagttga ttgaacaccg tttaaaagag gttgtgacaa aagtcttgtc
    43021 gcaacctctt ttttaaccaa aaaagtgcaa atagtttact ttaagtaaga gaataattat
    43081 gatgaataaa agtggaacaa atagcatact atatatattg aaatgaaaac tgtatggatg
    43141 ctccaacttg atgaagctaa ggaggaattc gtatgtcaga aaaggaatta atgaaggtag
    43201 tcggttttta tgaagggttg tcgatcgatg atccaaaaag ctttattgat gaaaaagaac
    43261 agatacctgt acccagttca cgggatcttc tggtaaaagt caacgcagta tctgttaatc
    43321 ctgtcgatac gaaacttcgc caagataacg ggataagaaa tgctttacgt atcttagggt
    43381 ttgatggagt cgggaaagtt gttgctgtag gtgatgaagt gcaaaaattt tctgtcggcg
    43441 atcgcgtttt ttacgcaggt acaacgacaa gagcaggaag caatcaagaa taccaactgg
    43501 ttgacgaacg tattgtcgct cttgcaccaa aaaatctttc agatgaagaa gccgcagcct
    43561 tgccgttgac ctcattgacc gcttatgaat tgttatttga aaaatttggt ctgactccag
    43621 aagaaaatgc caaccgcggg aaaaaaatct tagtgatcaa cggagctggg ggcgtcggtt
    43681 cgattttgaa tcaactagcg cattgggctg gcctagaagt ctacgcgact gcaagtccga
    43741 agaattttga atggctgaaa aaaacaggtg tagactatcc aatcgattat catcacgact
    43801 taagtaaaag ttttcgtgaa ttaggaatcg atcaagtaga gtatgtcgct gtattattcg
    43861 atattacccg ctactttgat caaatcaaac accttgtccg accatttgga catgtgggaa
    43921 cgatcgtagg gatcaaagag ccgttagata taggtgtttg gaaaaaccaa tctatcagct
    43981 tcgactggga gtatatgttc gctaaaacag actataacta tgaaatcgaa acgcaaggag
    44041 aagctttagc gcacattgct gctttagcaa atgaagggat tatccgcagt acattgagca
    44101 aggtctattc agatgggatg aatgcgaaga acttgaaaca agcaaccaaa gacgtagaaa
    44161 caggacatat attaggaaaa gtcgttgtta gtggtccgtt caacggataa gcaagaatac
    44221 gccgattttt ttcttatttg actttgataa aaacaagagc gcatgacagt aatttgccat
    44281 aagctcttgt ttttttattt ataataagcg aatcaacgaa agggaaagaa ttcctaccaa
    44341 gacgatcacc aataagcttt ttgaaagaat agcagctaaa agtgtaggaa ctgaagccaa
    44401 agcattttcg agattgatct gcggcagatg tccgatattt tgggtaaata gactgctgaa
    44461 ccataaagca gacataatga cgatcgggac aaaacttaaa tactcaagca acggttgcgg
    44521 caaatcgaat tttttcagta agacaaatgg taggatccta gaaagccaag ttgctaaagt
    44581 acaacctaaa atagtcagca atgtaaactc aaaagaaggc atgtttgatc cacaccccca
    44641 atccgcaccc aatcagtgtt acaactaaaa tcactaagtt acttgggata aaaatcagtc
    44701 cgacatatac cagaatcaaa gtgagaccaa tcaggatcaa ctgtaatcgt ttagaggtgt
    44761 ttctatcaga aatgacttga agataaagta acccgataaa catagcgacg atggcgaaat
    44821 ccaaaccaaa ctttttcgga tcagtaataa aattccctaa aagtgcgcca acaagagaag
    44881 aacccaccca cgtcaaataa gaaatccaat tagctgtatt catccaacga aagtttaact
    44941 tttgtccagt atagttcaat ttattcattc ccagtgcgaa actttcatct gtcagcaatg
    45001 tgccaatcag cagattttga agcagccgat ttttcttgaa ataaggagca atcgtcatac
    45061 tcatcagaat cattcgggaa ttgactagaa aggtcgagag aacgatggag agcagtggac
    45121 tatggctagc aagcatgctg accgtgataa attgggcaga accagcatag atcagcaggg
    45181 acatcaacgt gacgaccagc ggatgaaacc cggcagcttt cccaacgata ccaaatgcaa
    45241 gtccaatacc gatataacca aaaacggtag gtaacgtgtc tttgatcgca gtttttatat
    45301 ctaacttttc atccaataac aaaactcctt tagcacgaca taaattttca taattgtcgt
    45361 caaacttgtg aatattctaa atttttaatg aagacttgtc aacttcaatt ttaaacaaat
    45421 aaaaaagact ggacgtagtt taaaaaaatc ttctcaagtt agatttccag acctaacttc
    45481 tgaggatcac tacatttgtc acagtctctt atactgacaa gtataaatga agtatgaaga
    45541 aaaagtattt gttattggta tttctcatta ttcttgtttt ctaatgtgtt cacttcaagt
    45601 ggaattttag aatattattc aactaacaag tctacatcgt atctaaatcc atttgtatca
    45661 cttaattgct tgtaaaattc gccacttttt ttaattgttc ttttttgagt agtaaggtcg
    45721 agtgaaacca gcccatatcg gtttttatag gcgttgatcc atgaccagca atcaataaat
    45781 gtccaaagat gatacccctt aatgttacat ccttcattta ttgccttcca taagtaagca
    45841 agatgatctt tgatatactc aatccgataa tcatcaatta tttgccctgt atcatccatg
    45901 aaacgttctt catccgcaac gcccattccg ttctccgaga taaatgcttc gatgtttcca
    45961 taatggttct tgatatccat gcacaaatca taaattcctt tttcgtaaat ttcccatccc
    46021 cggtattcgt tcattcgtcg tccaggcatg atatgtggtt cgaaaaacca ctctggtaaa
    46081 aatggactag atgtatccac catagaagaa cgagatttga cgcgacgtgg ctcgtaatag
    46141 tttagtccaa gaatttgagc ggtattttcg tagatacata caagatcttc tttcgtataa
    46201 tcttcaggaa gctgattata ttcttgtaat aacaggataa gctttttaga aaaattccct
    46261 ttaaccatag gatctaaaaa gctccgcgta taaaataaat cagcgatttc tgctgctttc
    46321 acatcagcag gattttgact acgaggaaaa gtaggactca aatttagaat aacgcctatt
    46381 ttactaggaa gagctagttc tttaaatact tgtattgctt ttgcgtgtgc aagtatagtg
    46441 aagaatgctg cttgcgctcc gcgtttaaag tcaatgaggt ttggataatg aaaatcttca
    46501 agataagtac ctaaaatagg cccaagtggt tcattgaagg taaaccaatg acaaactaag
    46561 tcaccaaatt ctttaaaaca agtttcagcg taatgaacat aagcatcaac tacttcacgt
    46621 gattcccatc ctcctttatc ttgaagagta gcaggcatgt caaaatgata taagttggca
    46681 aatactttga ttccgttatt attcatttcg gaaaataatt ttcgatagaa agaaatggct
    46741 ttgggattta cttttccggt accgccattt ggaaacattc gtgcccaaga aatagaaacg
    46801 cgaaaggagt tatgcccagt ttttttcatt agttctatat cttctttata ttgatggatg
    46861 tgatcggttg taatagagga atcgattcgt tgataaaacc ggtaaggttg ttctttaaac
    46921 cagcgatccc agatagtctc tgcttttcct gtttctcctt ttccttctgc ttgctcagct
    46981 gaccaagcgg aaccccacca aaaatcatct ggaaaagtta gctttttcat agttgtcata
    47041 atttttttac ctcctgtttc cacttaatga taacaccgct tacaacttaa agtaaatagt
    47101 tttgtttaaa tatatatatc ttttttattg tgtgtataat tttgatgtgg attttatcgg
    47161 tttatttatg ttaaagtata gatgaagata tgtttttgat tggagggtca ccattgaaaa
    47221 aaattgcgaa gtatatggaa atttacttag atataaagaa aaaaattgta aatggagagt
    47281 ataaaattgg tgagaaactt cctacaggag atgaattggc agttatttat catacaagca
    47341 agttaacagt aaagaaaggc gtagacttac ttgtttccga aggagttttg cgaagtagaa
    47401 gtgggtatgg gactgaagtc gtcagaacac caattgataa ctcacgagtt cttggtccaa
    47461 acgaaggttt gtttagcgtg gttggtgaag aacacgtaga ttcagaaatc catactttct
    47521 caattgaact tccatcgaaa aaagtagctg ataaattaag tatcagtgta aaagactatg
    47581 tatataatat tgtacgaagc cgttttattg attgtcaacc atactcgatt gagcaaacat
    47641 tcatgccatt agcagtcata ccaggcttgg aaccaaaaca tttaaagaaa tctgtatatg
    47701 cttatatcac aaatgaactg ggtctggaaa taaagacttc gcacatacgt atcaaaggag
    47761 atactgcgaa tgaatttgac gcaaaaatat taaaaataga tccaggtgaa tttatgattg
    47821 agatagacaa agttgttgct ctatcgtctg gtaaaccttt cgagtattcg gtatcgagac
    47881 atttatatca agactttgtt tttgaagctg tttttgttga aaaataagct cgggaaaatt
    47941 attttttcag agcttatttt tttatactaa aaaagatata tatatttaat taaaagtatt
    48001 tacttttttt gataagtgat gtattatttg ggtggaaaaa gtaatcgatt acaataagca
    48061 taaaggagaa aaaatatggg caaaagtagc aggaaggaca ttgtatttga aaaatttggt
    48121 atagttgcga ctaagttggg gaatcagatc catttaagaa ctttgcgaga tgcgtttgct
    48181 acatttatgc catttatgat gttagcagga tttgtgacac ttattaattc agtgatctta
    48241 gaaccaacag gattcatggg ggaaattgtc gatactagta cattgaccac gttacaacaa
    48301 attggtactt caattgccaa tggtacatta agcataacta cattattagt tgtagcagca
    48361 gtatcttatc atatgtgtgc aagcagaaat tatacaaatc atattgcagc ggtattagtt
    48421 gctatttcga catttgttgt attaacacct atgcagatga tgtttactcc tgaaaatgcg
    48481 gataaagctg ttgaagtgac gggtgtgata cctatatcct atacaggagc ttcaggaatg
    48541 tttgtaggta tatttgttgg attagcggct acagaattat ttattaaact ttctaataat
    48601 aagaaactac agattcagct atccggaaat attcctcccg ctgttttaaa gtcttttaat
    48661 gtattaatcc caatcattat tacgattacc tcgtttgctt tattgtcatt tataatcatt
    48721 caattgttta atatggatat aaataagcta attaccacga tagttacaga gccattaagt
    48781 aaaattacaa ctggtttacc aggatttttg ttaattacat ctattgctaa tctatttttt
    48841 ggatttggta tccatcaagc agtaatatca ggttcattgt tagatccgtt tttgattcag
    48901 aatatgcaag aaaatatggc agcttatgca aacaaagagc aaattccaca tattatcaat
    48961 atggccttta aagatacctt tgcggtaatg ggtggatcgg gaaatacgat tgctttattg
    49021 attgcaatat ttatcttcag tcgtagaaaa gattataagg actttgctaa attatcggta
    49081 actcccgcta tattcaatat aagcgaacct attatttttg gattgcctat tgtatttaat
    49141 atcagtctga ttattccctt tgtgcttgca ccgatttttt ctttgacagt agcatatttt
    49201 gcaactgctg tgggattaat taatcatgta gtagtgcaaa ttccttggac tacgccacca
    49261 gttatatcgg gatttttagc aacagccgga gactggcgag cagctgtttt acaaatatta
    49321 attatagccg caagtgtgtt tatatattta ccgtttttac gaattgacga acacgtgaca
    49381 actaaattaa gtgaaaatga aacataattt actatgagat aacaaaaatt aacacaagaa
    49441 gtcgttggaa tataaatagc cagtaatttc aataaggatc atattttttg ataagtacga
    49501 acatgtagaa aaacaaa
//
LOCUS       DAYYCW010000010        41815 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000010 DAYYCW010000000
VERSION     DAYYCW010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.010"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(88..912)
                     /locus_tag="ACHAYA_000561"
     CDS             complement(88..912)
                     /locus_tag="ACHAYA_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM8604390.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(885..1565)
                     /locus_tag="ACHAYA_000562"
     CDS             complement(885..1565)
                     /locus_tag="ACHAYA_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8604391.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1588..2526)
                     /locus_tag="ACHAYA_000563"
     CDS             complement(1588..2526)
                     /locus_tag="ACHAYA_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8604392.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2771..3622)
                     /gene="ispE"
                     /locus_tag="ACHAYA_000564"
     CDS             complement(2771..3622)
                     /gene="ispE"
                     /locus_tag="ACHAYA_000564"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM8604393.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3776..6349)
                     /gene="mprF"
                     /locus_tag="ACHAYA_000565"
     CDS             complement(3776..6349)
                     /gene="mprF"
                     /locus_tag="ACHAYA_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8604394.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6490..7296
                     /gene="proC"
                     /locus_tag="ACHAYA_000566"
     CDS             6490..7296
                     /gene="proC"
                     /locus_tag="ACHAYA_000566"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM8604395.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7375..8931)
                     /gene="rny"
                     /locus_tag="ACHAYA_000567"
     CDS             complement(7375..8931)
                     /gene="rny"
                     /locus_tag="ACHAYA_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM8604396.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9342..10391)
                     /gene="recA"
                     /locus_tag="ACHAYA_000568"
     CDS             complement(9342..10391)
                     /gene="recA"
                     /locus_tag="ACHAYA_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM8604397.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10500..11741)
                     /locus_tag="ACHAYA_000569"
     CDS             complement(10500..11741)
                     /locus_tag="ACHAYA_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM8604398.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11981..12559)
                     /gene="pgsA"
                     /locus_tag="ACHAYA_000570"
     CDS             complement(11981..12559)
                     /gene="pgsA"
                     /locus_tag="ACHAYA_000570"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM8604399.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12768..13691)
                     /locus_tag="ACHAYA_000571"
     CDS             complement(12768..13691)
                     /locus_tag="ACHAYA_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM8604400.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14430..15716)
                     /locus_tag="ACHAYA_000572"
     CDS             complement(14430..15716)
                     /locus_tag="ACHAYA_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8604401.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15709..16977)
                     /locus_tag="ACHAYA_000573"
     CDS             complement(15709..16977)
                     /locus_tag="ACHAYA_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8604402.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17229..17690
                     /gene="mscL"
                     /locus_tag="ACHAYA_000574"
     CDS             17229..17690
                     /gene="mscL"
                     /locus_tag="ACHAYA_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM8604403.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17749..18195)
                     /locus_tag="ACHAYA_000575"
     CDS             complement(17749..18195)
                     /locus_tag="ACHAYA_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8604404.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18288..18533)
                     /locus_tag="ACHAYA_000576"
     CDS             complement(18288..18533)
                     /locus_tag="ACHAYA_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM8604405.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(18648..19226)
                     /locus_tag="ACHAYA_000577"
     CDS             complement(18648..19226)
                     /locus_tag="ACHAYA_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8604406.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(19303..19833)
                     /locus_tag="ACHAYA_000578"
     CDS             complement(19303..19833)
                     /locus_tag="ACHAYA_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM8604407.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(19837..20697)
                     /gene="rlmB"
                     /locus_tag="ACHAYA_000579"
     CDS             complement(19837..20697)
                     /gene="rlmB"
                     /locus_tag="ACHAYA_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM8604408.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(20690..21085)
                     /locus_tag="ACHAYA_000580"
     CDS             complement(20690..21085)
                     /locus_tag="ACHAYA_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM8604409.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(21082..22494)
                     /gene="cysS"
                     /locus_tag="ACHAYA_000581"
     CDS             complement(21082..22494)
                     /gene="cysS"
                     /locus_tag="ACHAYA_000581"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM8604410.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(22491..23027)
                     /gene="epsC"
                     /locus_tag="ACHAYA_000582"
     CDS             complement(22491..23027)
                     /gene="epsC"
                     /locus_tag="ACHAYA_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM8604411.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(23113..23352)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23349..24809)
                     /gene="gltX"
                     /locus_tag="ACHAYA_000583"
     CDS             complement(23349..24809)
                     /gene="gltX"
                     /locus_tag="ACHAYA_000583"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM8604412.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(24979..26136)
                     /locus_tag="ACHAYA_000584"
     CDS             complement(24979..26136)
                     /locus_tag="ACHAYA_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM8604413.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(26170..27540)
                     /gene="radA"
                     /locus_tag="ACHAYA_000585"
     CDS             complement(26170..27540)
                     /gene="radA"
                     /locus_tag="ACHAYA_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM8604414.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(27603..28130)
                     /locus_tag="ACHAYA_000586"
     CDS             complement(27603..28130)
                     /locus_tag="ACHAYA_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM8604415.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            28360..29166
                     /gene="proB"
                     /locus_tag="ACHAYA_000587"
     CDS             28360..29166
                     /gene="proB"
                     /locus_tag="ACHAYA_000587"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM8604416.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            29163..30410
                     /locus_tag="ACHAYA_000588"
     CDS             29163..30410
                     /locus_tag="ACHAYA_000588"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM8604417.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            30486..30830
                     /locus_tag="ACHAYA_000589"
     CDS             30486..30830
                     /locus_tag="ACHAYA_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604418.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(30947..31273)
                     /locus_tag="ACHAYA_000590"
     CDS             complement(30947..31273)
                     /locus_tag="ACHAYA_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604419.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(31888..33090)
                     /locus_tag="ACHAYA_000591"
     CDS             complement(31888..33090)
                     /locus_tag="ACHAYA_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8604420.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            33207..33722
                     /locus_tag="ACHAYA_000592"
     CDS             33207..33722
                     /locus_tag="ACHAYA_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8604421.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(33921..34367)
                     /locus_tag="ACHAYA_000593"
     CDS             complement(33921..34367)
                     /locus_tag="ACHAYA_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8604422.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(34414..35892)
                     /gene="msr(C)"
                     /locus_tag="ACHAYA_000594"
     CDS             complement(34414..35892)
                     /gene="msr(C)"
                     /locus_tag="ACHAYA_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8604423.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(36246..36875)
                     /locus_tag="ACHAYA_000595"
     CDS             complement(36246..36875)
                     /locus_tag="ACHAYA_000595"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8604424.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(36859..37323)
                     /locus_tag="ACHAYA_000596"
     CDS             complement(36859..37323)
                     /locus_tag="ACHAYA_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8604425.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(37810..39078)
                     /locus_tag="ACHAYA_000597"
     CDS             complement(37810..39078)
                     /locus_tag="ACHAYA_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8604426.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(39309..40046)
                     /locus_tag="ACHAYA_000598"
     CDS             complement(39309..40046)
                     /locus_tag="ACHAYA_000598"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8604427.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(40113..40682)
                     /locus_tag="ACHAYA_000599"
     CDS             complement(40113..40682)
                     /locus_tag="ACHAYA_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8604428.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            40810..41682
                     /locus_tag="ACHAYA_000600"
     CDS             40810..41682
                     /locus_tag="ACHAYA_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8604429.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
BASE COUNT        11856 a         9007 c         7442 g        13510 t
ORIGIN      
        1 aaataagaaa atagttcggt atttattaaa ataaaaagaa gcctataaaa aatttttcgt
       61 ttttataagg cttcttttct ttataattta gttatttttt cttctgaagg caaccaatat
      121 tcgtttgatg atatttacaa tcaggaacaa agcaataaag actaatgtga ttgttgctcc
      181 tggaggtgta tctagataaa acgagctagt tagtcctgac agcatcccta gaaatcctac
      241 acaaacactg atagcaatca cgacattgaa gcttttgcct aatcgcatac cgattgctgc
      301 gggaagaacc atgattgcgg atatcaaaag tgcaccggca atcgggatca tcacagcaat
      361 agccacacca gtaatcacat tgaacatcat cgacatcaaa tgaacgggta gcccatccac
      421 gtgggcggta tcttcatcaa aagtcagaac atacatcggc cgtttgaata aaaagaacaa
      481 tataccgatg gcaacgaata agattcctaa aaagatgact tgctcccatg taatggtcac
      541 gatcgaacca aacagatagg attggatact tgttgctgaa ttccctccgc tcaaattcat
      601 caatactaac gctagcgcca aaccaccagc cattaaaatg gcaatggaga tttctgaata
      661 ggttcggtag atcgttcgca gatactccaa tatcacagct gctaatacaa cgactagcat
      721 ggtcgtaagt gtaggattaa ggttcaaaaa gaatcctaac gctacccctg ctaaagagac
      781 atgagaaagc gtatcagcca tcagcgattg tcttctgatc actaaaaaca cacctagcat
      841 cggggcgatt ccggcaatga agatagcggc aagaaacgct cttctcatga attcatatga
      901 aagcaacgcc attgagaatc ctcctttctc accagtcgga tctgtctgtc cgcatactgt
      961 ttgatatctt catgatcatg ggtgatcatt agaactgatt tcccatggac atgcgcacta
     1021 tgtcggagaa gacgataaaa ttcatttctg gattcttcat ccatacctgt tgttggttca
     1081 tcaagaacaa ataagtcagg gtcagtagca aaaatacgcg ctaaactgat tcgctgcttt
     1141 tgtccgccag acaattcacc tattcgccga tttcgcatat cccacatccc tacagcttct
     1201 aaagcacgct gtacatgttg atgatctttt tcagagagac gcttgaacca gcggcctctt
     1261 ggatatcttc ctgaacggac taattcgatc accgtactag gaaaacctgc attgaaagat
     1321 gcaacttgtt gaggaatata accgatactt aatttttttc cttcttgatt tttctcagca
     1381 atctttatca ttccttttga aggtttcagt aatcctaacg atgctttcac caaggttgac
     1441 ttggctgctc cattttctcc agttaaaatg acaaattcac ccgcatctac atgataagag
     1501 acatcttcta gtacaggttc atcatcatag taaaaggtca aatcagatat ttcgatataa
     1561 cgcaccttac aacctccatt ctaaaactta attgatactt tcttttaaag ctttcagatt
     1621 tttttccatg acagaaatat aattttcgcc tgctttgatt tgttcattag tcaagctttc
     1681 taaaggattc aacacttcta gcttcactcc tgtttcattc gacaatgtct cagctacctt
     1741 cgaagaggca ttttcttcaa aataaatcac tttgatgttg tttttcttca caaattcttt
     1801 tagctcagct aagcgacttg gtgtgggttc ctgatctggt gaaacgcccg aaatcgctac
     1861 ttgttttaag tcgtattgat tcgctaaata agcaaaagca gcatgctgag tgacaaatgt
     1921 gcggtttgtt gcatctttta gctcatcgga gtatttttga tccagtgctt caagcttttt
     1981 gatataagca tcggcattct tcgtaaaagt ttccttttta tctggatatt tttcgcctaa
     2041 ttcttcagca atcgttctga cttcttttat agctaatacc ggatcgagcc aaacatgtgg
     2101 atcgtattca tgtgaatgac catgcccttc ttctgattct tcatgttcgt ctgcttcttg
     2161 cgattctaaa cggtcgattc ctttagctgc ttcgatcatc aaggtctgtt tgctatccac
     2221 tgaatccttt aacgaatcca caaaaaattc catgtctgaa ctattataca cgaaaacatc
     2281 tgcatcggaa atttctgctc tttcttttgc acttggttcg aaatcatgcg gctccgttcc
     2341 agcagggatc aaaagtttca cgtttccttc atcccctacg atctcttttg taaaatcata
     2401 catcggataa aaagtcgtaa cgatcgtcaa atcctctttt ttgtctgctt ccttgttcgt
     2461 atttccacat gctgcaaaca gtaaagcacc tatcattacc gtagctgtca aaagatattt
     2521 tttcatttat atgctccttt caatgaacaa atcggaattt tacgatttat taacgtgaac
     2581 taatgtatca aaataaaaaa acacagtcaa tagaaatcca tcaatttctg atttaagcat
     2641 gtatattctt gattaaatcg taaaatggat gagttgctgc tcatatctag ccatttcact
     2701 acaataaaaa actgaagaat accattttag gattcctcag tttttcatca cctattttac
     2761 ttcttatatt ttattgcagg gttcgtacta aataaacttc ctcacaaaaa ccttttaacc
     2821 cattatatac tctctgcgct cttgaataat gttgacacaa ggcaaaaacg gttggtccgc
     2881 ttccactcat caatgcagca tctgctccgt acttgatcat acgctctttg atttgatgga
     2941 tgactggatg ccgctttccc gttatatctt ctaaactgtt tcccattttt ttgatcatct
     3001 gctgataatc ttgctctcgg atggcttcag caagtccgtg gatgtcttga tgttggatgg
     3061 cttgcgtatc tacttctcta aacactgtac gtgtagagac actcattctt ggtttgacca
     3121 acacaaccca gcactgtggc atagaaggta atacttggat ttcttcccct ttgccaccga
     3181 caaatgccgt acttccgtag acacagtaag gaacatctgt accgacttcc aaaccgattt
     3241 ctgccagttc ttctaacgaa cagcccagat tccataattg gttgatccct cttagagcag
     3301 ctgcacagtc tgtacttcct ccgcctaatc cagctgctac tggaatattc ttcttaacaa
     3361 agatcttgac accggttttg atattgtatt tattttttag caataaagca gcttgataaa
     3421 cattgttccg ttcatctacc ggtaaaaatg ctttatttgt ttctacaatg attttgtctt
     3481 ctgttatttc ttcaaaaaat agatgatctg ccaagtctac gctcgacatc accatttcca
     3541 actcatgata cccatcttgc cgtctatata atacatccag tcctaaattt atcttggctg
     3601 gcgctttttc aatcaattcc atcctctcac ccattcgaaa agtctaagtc gcaagtattc
     3661 tatcataggg aaaaataaaa gtatagctta tgtctgccta gtggttgatt ttgtcatcgt
     3721 ttcaacagct ttcacagaat tttctcttca taatccaaac aagttctagt gattcttata
     3781 attctgtgag cacttcttct gccaaactcg gaacatttcc tttttctttt cctatcagac
     3841 tgagtagctg catggtagca aatccgaaat tacttccttt cctataagca aaataacgtc
     3901 cttcccaatg atcgacatat tttaatttct tttcataagc agactggaag gaatagaaaa
     3961 aattaccata attatatacg atatgcatga ctttttcttg aaagaaagaa aaatctgttt
     4021 ctccaacatt agctaatgga gctaagccta gattggcttc cgagtagccc attttctcgt
     4081 atacaataaa cagattggct aacagatagt cagctaattc ctcagattct ttaggtaaga
     4141 cccgcaacaa atcatatccc gcctgcttct tcgtcatcgg ctgagcggtg atgaagccaa
     4201 cgactttttg atctttacgg acaataccga caccgctaga taatacatat gatttatcaa
     4261 atcgtcccac agagaagtat ttttctcgtg caccttgaag ccatttttgt gaaacttcat
     4321 ttacagcttg atgtaattct tccggcagtt gatcgtacca agtaaaatgg tatccttctt
     4381 tcatcaaacg ttgatattca agttgctgac tcagccgaga caagtcttct gttgctagat
     4441 caacaatccc tgcttcacct actttcgtga agtgaaaacc caattcatga agcatcacta
     4501 cgtagctttc tgatatctta taaaacgcaa gctgatagcc taaacaatct gcttgtttca
     4561 taaactgatt cgttgctgcc cgcatcattt taggattgcc aactggatct cctaaaacaa
     4621 agcatttgtt tcctttgatc tgataaccaa aaacgacttg atcttggtcg tcttcttggt
     4681 aataaaaata actgtaacct gacaagcgaa gctgatggct tgtagctgtg cctccgtatg
     4741 tttccaataa acggtcaaaa cggtcttttt cgtactcact gccaagcttc gtagtatctt
     4801 cagctaaata ttgataaagc gccactaaag ctagcaatga gatgcccaaa ccaataagcc
     4861 ctgagaacca cacatcatct gaaggaaaca aaataaaatg atttcctgtt attcgaccga
     4921 ctaaaaagcg tccgctgtga tagcctgcaa ttgcgtaaac gataaacaaa aagccgaaca
     4981 agctagcatc aaagattata gctccccaag aataaacgaa ttttgttcga taaaattctt
     5041 ttctggataa ccacacagct aacagaatca agacatatac caatatcaac tgccaagata
     5101 acgtacgtgt gattgtattt aaaataccaa aagatagtaa agcaatcgta ggcagaaatg
     5161 cctttttgac ttttactgaa agtgctctag caaggccgag taataaaaag ccaaccaata
     5221 gattcactgt ctgatctaaa aaattgaatg agaacggcaa taatacttga aatagccgac
     5281 tgattgcaga cagattagtg attgttgata gaagtaccat catgatcccc gcaaaataga
     5341 cagcacttac gagcacgata tgcgcaacct tttggaaaaa cagtcgcgga atgttatcta
     5401 aaaatcgatt gattttgact cctgtatgtg tgatgaacaa tagaagtccc gtcataaaag
     5461 gtagaacata ataaaacaac cgataatata atagccaaac tactgctaca tcttgccgaa
     5521 cagacaattg actcattcct aagatcatca aaacgtcaaa agtccccatt ccacctggga
     5581 ccatcgtcag cattcctatc aatgtagcaa tcatgaacat aggatagatc gaaagcatcg
     5641 aaatatctat tctcatcaac ttgccgatga tcaaaaatac cgcaagggct cccgtccatt
     5701 gtccaaagga tgccagtagc agaccaagca agccttttgg ataaaactcg aagaacaatg
     5761 tctttctctt tagataagca aaaaagaaaa tgactggtgc taaaaaacta ccgccgaaaa
     5821 gccagatcca atagcttcga aaaatacttt ctgactgaat aaaaaagaca tcaataaatg
     5881 taagaaacga ccatacagac aaaccagtca gcataaatag tgcgactttt gatacagtgg
     5941 ctaaaaccat ttttctattt gtctgctttc cataaaaact agcccgcaga cttgctccta
     6001 ccacaccacc aaaaccggcg agattgttga tcgtgtttgt tacccacgca gcaagaagaa
     6061 actcttttct ttccattttt ggttttcctt gtttttctaa gactctgatc gtcacccaat
     6121 catacaacaa catcggagtg acacccagca gtccagccag aatcatgagc aggatcgtca
     6181 tgcggctttg ttgtcccatc ctttgccaaa tatcttgcca actcatgcca ttagcaatat
     6241 ttgcgacttg atttgccaca aagactaaca cggagccgaa aaaaatcagt tttaaaatca
     6301 gactatgttc ctttatccat tgcagcatca agtatcgttt atttttcaca ttttcccatt
     6361 cctaacttgt atacttttgt gtcatacccc aatgttatct tatttatttt aatttttcca
     6421 ctttagacct gttctttgct tgcgatgata gaaaaagaaa gatatcctaa tgctaaagga
     6481 gaggataaca tgaaagtagg atttatcggc acagggaata tggcacaagc tattattgat
     6541 ggtttattaa aaaaaggaac agtctcgaaa gaagaggtgt tcgtttccag tggacatttt
     6601 gaaaatgcac aaaaatttgc tgctgaaact ggcgtccatg catgcaaaag taatcaggaa
     6661 gttgcatcac aatcagatat cgttctttta gccgtgaaac caaaaatcat tgaaaaagta
     6721 ttggaagaac taagagaata tagccagaaa gtcttattca tttctattgc ttcaggaaaa
     6781 tcattaatag atttagaaac agcgcttgaa gatcctcagg cctccatcgt acgtgtgatg
     6841 ccgaatgtca atgtcagcgt aggagcaggc gtttctgcga tttgcaaaaa tgaagctgcc
     6901 acggaggaac agcttcagca agctacgatg atttttgaag caatcggcag cgtctatcct
     6961 ttagcagaat cagatttcag tacattcatt ggccttgctg gcagctcacc cgcattcgtt
     7021 tacatgctga tcgatgcgat gggacggact ggtgtactga atggtttgtc caaacagaca
     7081 gccactgaga ttgctgcaaa agcaattatg ggttcttgtc aaaaattcct ttcaagtgat
     7141 aaaaatccat gggaacttgt agaccaagtt tcttctcctg gcggtacaac tgttgcaggt
     7201 gtagttgcct tagaagaagc aggattcatc ccagcaatca tcaaaggaat caatacgacg
     7261 atcgagaaag atcttgagat gttgaataaa aaataatgat agcaatgttt tttcgtgcct
     7321 tacaaaaaaa gaagagctgt ggcttttgcc gcggctcttc ttttgtattt tttattattt
     7381 agcataatca gttgcacgag tttcccgaat aacgattact ttgatgtgtc caggataatc
     7441 cagttcttct tcgattcgtt tacgaatatc acgtactaaa cgaacagcgt ctaaatccga
     7501 gatttcttct ggcttgacca ttacccgaac ttcgcgtcca gcttgaactg cgtaacttga
     7561 ttctacaccg gcgaagctgt tcgaaatatt ttccagactt cttaaacgac ggatgtagtt
     7621 ttctaatgat tcacttcgcg caccaggtct tgctgctgag agcgcatctg ctgcagctac
     7681 aaggacagag ataacagaag tagcttccac atctccatga tgagaagcga tcgcgttgat
     7741 tactactggg ttttctttgt acttggcagc taattccgca ccgatctcaa cgtgagatcc
     7801 ttcaatctca tggtcaagtg ctttcccaat atcatggagc aacccggcac gttttgctaa
     7861 ttggatatct tcgccaagct ctgctgctaa gataccagct aattttgcaa cctcgaccga
     7921 atgcttcaag acgttttgtc cataactagt tctgaatcgc agacgtccga tgatcttgat
     7981 tagatctgga tgaagtgtat gagcaccgac ttcaaacgct gcctcttccc cgtattcgcg
     8041 aatacgttca tccatttctt tacgagattt ctcgaccatt tcttcgatac gcgctggatg
     8101 gattcgtcca tcttgtatca gtttttccaa ggtcatccgt gcgatttctc ttcggattgg
     8161 atcgaatccg gaaagaacaa ctgcttccgg tgtatcatcg atgattaaat cgataccagt
     8221 cagcgtctca agcgttcgaa tattacgacc ttctcgtcca atgatacgac ctttcatttc
     8281 atcattcgga agggtaacga ctgaaacagt cgtttctgaa acttgatctg ctgcgcagcg
     8341 ttgaatcgct aatgataata agtttttcgc tttgcgatct gcttcttctt tggcacgctg
     8401 ttcagattct tttaccatta gagttaactc atgattcagc tcttcttcag tcgatttcat
     8461 gatgacatct cgtgcgtcct cttttgataa accagcaatt ttttctagtt ctttttgttg
     8521 atcttcaatc attttttcaa cgtctttttc tcgctcttca attaattgct gttttgaact
     8581 aagtgcttct tctttacttt caagtgagtt ctcgcgtttt tctaacgagt catctttacg
     8641 attcagcgtt tgttcgcgtt gaatcaaacg attctcttga gattttaatt ctaacttact
     8701 ttctctcagc tcactttcaa cttcagaacg gtatttctga ttttcttcct tagcttctaa
     8761 caatgcttct ttttttagag tttctgcttc tttttttgca ctatcgatga ttccagcagc
     8821 tgatgattgt gcaccagcta tttctttctc gtgacgtgac tttgcgacca taaaccctaa
     8881 accaagaccg acaattaaac cgatgatagc gaggagaatg ttgaatacca taaaatccac
     8941 ctccatacta tctttttcat ttttatcatc tatacagctt ctatgataaa ttattctaaa
     9001 ctcgtcaata tgcatcttcg catatatgtg tacaaaaaac atgcacaact ttattctaat
     9061 gtttgtaccc cgtagtgtca actttaaata aacaaagaaa cggctttcta cttattcatt
     9121 aaacgatggt ttttaaaata aatgacacta gtttcatata ggtaaacctt taaattgaga
     9181 aacttttcta ttaaattatt ctttgtagat gaacagatat ttttatttta tgtacccact
     9241 cgtataatta aataactcac gatttcttta ttcaataaaa aaacggcagg tcaaggagag
     9301 tacctactct tcagaccagc cgtttttgtt tatcataatt tttattcttc tactaaaggc
     9361 aattcttctt gtcctttttc atcttctggg acagcgactt cttcaccaat accataagct
     9421 gctctaacta gagcagaaac ttctgccatc atttcaggat gattagccat atagatttta
     9481 gcgttttccc gtccttgacc aatccgatct tctttataag aataccaagc accgctttta
     9541 tcaacaatgt ctttttctac tgccatgtct agaagttctc cttcttgtga gatccctaaa
     9601 ccatacatga catctacttc agcgattttg aaaggaggtg ctactttgtt ttttacgact
     9661 ttgatctttg tacggttacc aacaatatct gtaccttgtt tcaattgttc tgcacgacgt
     9721 acttccaaac ggatcgttgc gtagaatttc aatgcgcgtc ctccaggagt gatttctgga
     9781 ttcccaaaca tcacgcctac tttttcacgg atttggttga tgaaaatagc gattgttttt
     9841 gttttgttga tcgaaccaga gagtttacgc aatgcttgag acatcaaacg tgcttgtaac
     9901 ccgacatgtg agtcacccat ttcgccgtcg atttctgctc gtggaactaa agcagcaact
     9961 gaatcgacta ctacgatatc tacagcccca cttgatacta aagcatcagc gatctctaga
    10021 ccttgttctc ctgtgtcagg ctgtgaaaga agtagttcat cgatattcac acctaatttt
    10081 tgtgcatatt gcggatctaa cgcatgctca gcatcaatga aagcggccgt tccgccattt
    10141 ttttgtactt ctgcaatagc gtgtagtgca actgttgttt tacctgaact ttcaggtcca
    10201 tatacttcga tgatacgtcc acgggggtaa ccgccgacac ctaaagcgac atctaacgca
    10261 agagagccac ttggaattgt agagatttgt tggtcgattt tttcgcccaa tttcataata
    10321 gagcctttac catagctctt ttcaattttt ttcagtgcag catctaaggc tgctttacga
    10381 tcatctgcca atacgataat cctccttata tttacaaatc agagcaacat ttcattatcc
    10441 ttatcatatc ttttttagtg aaaaaaagca agaaaaaacc gaacaaatat tcgcatttat
    10501 cattttctta aaagctgttt tctcaaaaga tcaaatcctt gcataaccgc attatatctg
    10561 atagcagcac gatctctggc aaagtgatat ttttccgaaa ccacgccgtc tttagatgaa
    10621 atagcgatcc atactgttcc tgcagattga ccttctaacg tgtccggtcc agccactccg
    10681 gtgaaagcta cactgtaatc tgttcttgct atctcacgtg cttgaatggc catctgctcc
    10741 acacattctt tgctgactgt tccatattct gctaatagct ccggatcaat ctccagaaaa
    10801 ccagcttttg tttggagcga gtaggtaacg aatccgcctg gaaatacttc agatacacca
    10861 gcaatgtcgc ctaatgcggc ttggaaggcg ccggctgtca aactttccgc tgctgttaca
    10921 gtctgcttgt tttctttcag tagttccact acgacttttg ctagcgaatt atcatcgccg
    10981 tatccataaa aatattcccc tactcgttct tggatttttt cttctaatgc taaaagagct
    11041 tgattccctg cctgaacatc atttgttttc actgtcagtc gtaatgtcac ttcatttggt
    11101 ttggcatatg gtgcgatcgt tggattgatc tgtgtctcaa tcagatcttt tagctcagtg
    11161 accagtcgcg attcaccaat cccataaaag cgcaaaactt tagaaattag tttttcttca
    11221 gaaggaaaac gttcctctag taaaggacgt acttgttcca caaacattgg tttcaactca
    11281 ttaggcggac cgggaagaag gatatacgca tgtgtatctg tttgataaaa agtccctaaa
    11341 gcaagccctg tacgatttgg tagaggaacc ccgccttcga taatctggga ctgttgcaga
    11401 ttgtttttgg tcatttttcg atgggttgtt tcaaaatatg ccaataattt tttgtatcct
    11461 tccgtatttt gtatgagaga cttaccgagg tgagccgcag ttgcttcttt tgtcaggtca
    11521 tcttctgtcg gtcctaatcc gccacataag agtatgagtt cacttcttgt ttcagctaga
    11581 gcaatcaact cttctaaacg ctgctgatta tctcctacaa ctgtttgata ataaacttcg
    11641 atacctagat cagctagctg ttctgacaag aaagtggcat tcgtattcac gacttgtcct
    11701 agtaaaagtt ctgttccgac agctattatt tctgctttca ttacttcccg ctcccttata
    11761 tagtatacgc tgtattttta tattttttta tttaaaaaga gggcaaggag ctgtgacaaa
    11821 aatcttgtca cagctccttg ttccaaataa acagtgttct cctgccaaaa cctcggtcat
    11881 aagtcacaat atacataaaa catgaaaagc tgttttttga aattatccct tgtgactcag
    11941 agttagacgg caggctcaca acctctccac taaacgatac ttacattgat cctttgaaca
    12001 caccgctgtt tttgatgaag taatccacac ctgaatagat agtaaagatc agacaagcat
    12061 aaagcatgat ttgatctact ggaataccga tcaggctgaa tgggatatta ttgataaaca
    12121 gcaagataat agcaaccatt tgtgtagccg tcttgacttt tcccggccaa gctgcagcca
    12181 tgacttctcc atgttcgaca agtagtaaac gcaagcctgt gacggctaat tcacgacaga
    12241 caatgatcgc tactacccaa ccaggggcct ttccttggtc aaccaacatg ataaaagcag
    12301 tcataactag cattttatct gccaacgggt ccgcaaactt gccaaaatta gtgacaaggc
    12361 ctcgcgcacg ggcaattttt ccatctaacc agtctgtgat actagctaaa gcaaaaataa
    12421 ctgcacctac tagctgtgtg acagccagtg tggtgtctcc tacgttaaat tgtccccaat
    12481 ctaaaggtaa acctacaacc aaaatgaaaa tcgggatcat acaaattcta agtacagtta
    12541 atttatttgg taaattcaca ctatcgactc ctttcttccc tattctacgt gactatcagc
    12601 gaagtttcca ccgatattat ttacttttcc aaatgaattt attcaaagga gtatttttat
    12661 tgaaattgat ctttttgaat taacgaagaa ggaccgcgac acagatttgt cacgatcctt
    12721 cttttttata cctacaagta aacagatgcg taaactaatt tatacgatca ttctgcgtat
    12781 tgaagagtaa ggttcacatt ttttggactc tgattgtttt cccctggatt aactggtacg
    12841 gattctccat tcgctttgat cgttgcattg cttgctgcac caatcgtgat ggtcgcattt
    12901 gttgctgttt ccggtaacac ggtactctgt gtttcatttg ctgccagtgt cccttgataa
    12961 acataggcat tgtctactag cacacctaac catacacgat tgacagcagt aaattcaagg
    13021 gttacaggct tggtagcgtc tgtgatattt actgcgatgg cactgccaga atcttctcct
    13081 gtagctattg ccattttctt ttcttcactt gattttgttg ttgattctac tgtagaagac
    13141 tctttggtct gcgtgctttg ctctgaggaa gaggactcct cgactgatcc ttctacttta
    13201 agcgaagaag ctgtgtctcc gattattgga tcagcttgac gatcttgcca agtcatatag
    13261 cccacgacaa cgacaatcgc caaagcaacc aatcctaata aaatgacagg aagacttgtc
    13321 caaaagcgac ttggatgcga ttcttcctga tgcattgctt ttctagaagt acttactgtt
    13381 tcaggctgcg gacgttcagg gaccgatcct gtcaatttgc ttttcccatc aaaaacatcg
    13441 actaaatggt ctccatcttc tcctacagct tcagcgtatt gccgaataaa tgcccgcacg
    13501 taaaatttac caggcaattg gtcaaaatca tcattgtcga ttgcttccaa ataacgtttt
    13561 tgaatttttg taatctgctg taattcatct aaagatattt ttttattcag gcgtgcttgc
    13621 cgtaattttt ctccgatggt ttcaaaagcc actcgttcat cttctccagt ctgtttgatt
    13681 ctccgaagca tctgctgttg caacgaaaga cgttatgttt gggtgctgtg acaaaagtca
    13741 tgtcacagct ccttttttcc gaataaacat tgttctcctg ccaactcctc ggtcacaagt
    13801 cacaatatcc aataaatata agaagctgtt ttttgaactt gttccttgtg actcggagtt
    13861 aaacggcagg ctcacgacct ctttcttaaa cggtgttcaa tcagctgttc ctcggtatta
    13921 gctcaaaaac cggcaaaata taaggagata tttttccgta tgtttctcta atactcaggt
    13981 caaaacgctg atttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta
    14041 ataagtccaa ccaagttaaa aacattaaga agctgttttt acttggctgt tcttattact
    14101 tgatgcaggg cagctttttc acaacctctt ggtacacggt gttccagaag tagcttcttc
    14161 ggcaataagc cgaaatatca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
    14221 tcttgaagct gaccacttct gtcacaacct ctttgattac ttcgtttgat tttattttaa
    14281 tataagttga ttaaaagaga gcatgatttg ttttattttc tcactaattt ttccaacaca
    14341 actaagaata ccataaaagc tgattttgga ggaggttcct actacaagtc taaaacgaaa
    14401 tttaagactt ttcctcataa aatactggtt tattttggtc tcatgtaaaa ctcgctgaag
    14461 gcctcttcac ttataaatgc ttccccagct gccagtacgt ctttcatttg gatcgaatcg
    14521 atgatttcag gaagatcaaa taaagttcga tcgccaaata aactttgcgt aaattgattg
    14581 gcaatatatt cgatcgaatt taatgattgg aaatattgtc caagcatttt cttcttcagc
    14641 aaatcgagat ttgtttctga cacttccgga tcatcagcga atccaagaat gatttctttg
    14701 actttttctg ctgctttctc tggttcgtcc gtatctccgc tgaagtccgc aaagtgaaat
    14761 tcacgatcca agctgaattc aaaaccaaag ctgtcatcga taattccttg attgtacatc
    14821 gctagatagt ttcgagaagt attgcctaaa atcatttgga acaacagatt gattgctgtt
    14881 ttatagcgta acagttctgt tccttcttgc ggcaatgtgt ctaatccttt aataccaagt
    14941 acaaatttat ctcgtgtaat ggctgcttcc aatgcacttt gtttgatgat ttcttttgtg
    15001 ttttcaggga aataacgtac aatttcttgc tttggaggga aattttttgc ttcttggttt
    15061 tcccgaataa gtttcatcaa tttctcaggc tccatcttac caactacaaa taaaaccata
    15121 ttgcttggct gataaaacgt ccgatagcac gtatacaagt cttgagctgt gattttgtcg
    15181 atactttcaa ccgtgcccgc aatatcgata tgcaatgggt gtgtaggata caagttattt
    15241 agtatgccaa aaaacatccg ccagttaggg tcgtcttcat acatttggat ttcctgaccg
    15301 atgattcctt tttccttgtt tacagtttct tctgtgaagt aaggtgcttg tacaaaatcg
    15361 atcagagtcg tcagattctt ttctacctga tccgtcgtag aaaacagata gctggttttc
    15421 gtaaaacttg tgaaagcatt ggcggaagct ccttgtttcc caaataattg aaagacatca
    15481 ccatcttctt tttcaaacaa cttatgttct aaaaaatggg caattccatc tggaactttc
    15541 accttttctt tttcaccata gggaataaat tcgttatcga tcgaaccata attcgtactg
    15601 aacaaaccat atgtcttgtg atagtcattt ttaggcaaca aatacacggt caaaccgttt
    15661 ggcagtactt catgataaag tgtttcattg atttgctcat attctgtttt attcatttgc
    15721 atttcctcct gctaaaaaga agattgcctg caattcgatt tgctcagcta cttgttgtat
    15781 ctctttgatc gttacgctct ccatacgttt caaccattct tcatcagata attttgtttc
    15841 cggcagccag ctgtccaaaa aagaagcttc gattgccgct tgcggagagt ctagagaaag
    15901 cagatattgg tttctcagca ttgcttttgt ctgagacagt tccaactctg ttatttcacc
    15961 atttcgcaaa ctttctaatt gctcgtgaat cagatgaagt acttgatttc ggttcttttc
    16021 atcgataccg gtttggacac tcatgaatcc tcggaacgta tccacgctac tagaggcata
    16081 ataagctaga ctttctttct ctctgacatt catgaaaagt ttagagtgag gaaaaccgcc
    16141 aaataaaccg ttaaagacca ttagcgcaaa acgttctggt tcatcgtaat acacatttgt
    16201 ctgataagct aggttcagct ttgcttgaac gattggctca cgtacttgtc gttccttcac
    16261 gatattcact tgaggttggg tataaaacag atctggataa actgtttccc gcgactcaaa
    16321 tggtaattgc ccaactgctt cagctgcttt gtccggatca acatcgccga caacaaaaat
    16381 gtcgatttga tcatttgcca tcatttcttg atagtaggac gctagcgaac tagcagtgat
    16441 cttgtccaaa tcttcgacta cgcccaaccc tgggatcttt tgttccgggc tgttttcaaa
    16501 ataaagttct tgcaaggcat aggaagcaaa agtttgttta tcttccttca aactttccaa
    16561 atatagacgc agattgttct tttccaagtc aaacgtcaac tgatcaaact gatgattttt
    16621 aatatttgga taaaataaaa cctcttttaa aaaatctacc gcttgagaaa aaaggtttgg
    16681 atcattgaca tattcaccat tcacaaaact gatccctgcg tttagccaat gtaaatttcc
    16741 tttttttcta actgataatc caaagcttgc gccataaagc tctgcaagtt gcgcacttaa
    16801 ctttgtctga tccgaataat tcaaactatt tgtttccaac atgctgctga gaagtgaacg
    16861 tttcgtgatg acttcttgtt gcagacgcgc agtaaaacgg ataaaaatcc ggatcgtttt
    16921 gtattgtttt gttggtaaga catgaagatt cacgccttct gccaattgat acaccatatt
    16981 tatatgctcc ttttcacagt tgattgcccg cttactcttt taatgggtca acctgccttt
    17041 tttacttatt cctctgcttg aaatttataa gttcaggcaa ttcgccgttt tttctaaatg
    17101 atgttacact ttatagtata gcatagatgt tttttatcaa aagaaacaga cggctaaaag
    17161 cgtatattgt ttttttattt tttatttttc gatatactaa agacaaataa aagaagggag
    17221 ggtcattcat gatcaaagaa ttcaaagagt tcatcatgcg tggagatgtc ttggatcttg
    17281 ctgtcggcgt tgttattggt agtgcgttta caagcatcgt aaaccaagtg gtgcaaggct
    17341 tgatcacacc actaataggt tggtttgtcg caatgatcac aggtacttct gatttagaag
    17401 gagcactttc aattcttgac tggtcgccga caaaaggcgt cacatttgcg tttggtaacg
    17461 ttattagtgc tatcattaca tttctgatca ctggttttgt cttattctta gttgtcaaag
    17521 cggcaaaccg tgcgaagaat ttacgctcca aagaagagca agtggaagtg gaagaaactc
    17581 ctactgcaga agattatctt agtgacatac gcgatcttct tgccaaacaa gtggcagaag
    17641 acaatacaca tccagtagag caaacaaatc tttccgatac ttctaaataa taaagaatct
    17701 aaaaaaaaga aagccgattt tcagtgtcgg ctttcttttt ttagattctt aacttgctac
    17761 ttgttccttt ttcatgtcga tatgtggaat atcatcttcc aaatacacat ctgagattgc
    17821 cttgaatcca aacgactcat aaaaagcttg taggtattct tgtgcgccga tcacgattgg
    17881 tttttctgga aaatttttct ggatcatgtt aatcgtttct tgaacaatct ttcttcctaa
    17941 accatcttta cggttgtcct gatgaacaag gacacgtcca aaatggatac tctccgcttc
    18001 ttcatatact cgcgtgtagg caaggatctt cccatcagta tcttgaaaaa acgtatgcca
    18061 agcttctcca tcaatgtcat caacttcttg ataaggacac tgttgttcca caacaaaaac
    18121 tgcgattcgt tctttgacga tcgcgtaaaa ctctgctgtt gacaattctt caaatttctt
    18181 tacctgatat tccactaagc aaacgccccc tgctttttta ttcttagcat accgattcgc
    18241 acacaaaaaa gcaaacgaac cttacgaatc tcaggctcgt ttgcttttta aactgcttca
    18301 gttaaaaatt cgatctcaac tgtccgagta agtacatctg aatagctgta agatactcgt
    18361 tcaaaagaat tttcttcagg atccaaatca acaacgaata ctgaaggata ggtttctgtt
    18421 aaaacgccgc ggcgttctgt ttgacgcttt cttcctgtct gagcaaccaa catgattggg
    18481 ctgccgatac gaccttctaa atctttcttg attgaagcta aagttgttgg catacagttc
    18541 acctctacta tcaactatac cacatatttt taaaattttc aagtttacca taaaaaaaaa
    18601 agaaatgcaa taaaaataaa taagaagctc attaagattt ttctaaatta ttcctcatta
    18661 taaaatttca agtgtttttt tattttgagt tttactctcc cgtaagcgct cattacttgc
    18721 ttgtgatcca ttgctaaaca ttcagccatg tctacaaaat caacgccttg catataataa
    18781 tgaaaaattt tagcttctat ttttgaaaac tggatttcgc aattttcgat tgcttctttt
    18841 aataaaagat gctgaattgc taaaggttct tgcaatgtga tctctgctaa aaagtctggt
    18901 ccttctgtct ctattttttg gtcgattgag acttcatctt tttgatcacg acgcttcagc
    18961 gcgttctgct tcctcaacag agaatagatt tgattgctta taacgctttt ataataactt
    19021 ccaaagctaa cacctttgct tttttcgtaa gttctcaatg cctgatataa agcgatcctt
    19081 gcttcttgtt cccaatcctc agaaacataa tttcttaaat aataggtgtt tttcaaacgt
    19141 agtaaaagtg gaaaatattt ttgatacagg gcagcaaatg cttcttcgtc accattacgt
    19201 gcagcgcgca ataactcctc agacataaaa taattcctcc ataaattcgt ttatggagga
    19261 atcttatcat caatgattca tcgaactgat tagaacgata tgttatttat tttttctcat
    19321 taaatcgtcc aatttttggg acaatttttt taactgctct tcattccatg gagaattacg
    19381 tcggaaatca ataaaatgca tatctttatt ttcttgggct attgtttttt ccgttttttt
    19441 tatcgtctta tataattcat aagcagaagt tcgtaatgct ccttgggaaa agatcatcca
    19501 ctgttctgct aaatcgcttg tcgccactgt cacttgtgtc aaacggtcat tcagctgacc
    19561 agcgattctc tcgatataac tgtcagccgt ttcgtcttct ttcgtaaaaa caacatccaa
    19621 ctggtatttc ttataatttt gttggatgcc cggaactagt tgggcatcga agacaacgat
    19681 gacttccatt ccttcatatt tcgcataatt tgataaacga tgtaaaagag cttctcttgc
    19741 atccgccatt ttgtcctgct ttttaagctg gacaagttcc ggccacgaac caatcatgtt
    19801 gtagccgtct acaatcaaca gctgtttttt catggtctac tccccctgac gttgggaaaa
    19861 agcctgatac ataagcaaac cagcagcaac actggcattt aagctctgga cgtgcccggt
    19921 catcgggatc gtcagcaatt catccatttc ttttttcaat cctggactca ttcctttgcc
    19981 ttcattacca atgactaaag caattgatcc tttggcattc catttttgga agtttgtgcc
    20041 ggtcatatct gtgccaaata cccaaaatcc tgtttctttg agctgttgca cagcttgtac
    20101 caaattcgtc acgcgtgcaa cagggacatg ttcgactgcc ccagtggaag tctttgttac
    20161 gataggtgtg attccaactg ctcggtgttt tggtatgatc acaccatcga cgccacttgc
    20221 gtctgctgtc cgtaaaatcg acccaaaatt gtgtggatct tcgatgctat ccaaaatcaa
    20281 aaagaagggg tcttcttttt tctcttttgt tcgttcaatc agttggtcaa gggataaata
    20341 ttcataaggt gtaatcgcaa gaacgactcc ttgatgcacg ccgtgatcgc tcaaagtttc
    20401 taatttttgt ttaggaaccc atttgactgg cacagcttgt tcacgtgcga tttctttcag
    20461 ttcttctact ttttctcctc gtgaatcttc ttgaagaaag agtttattcc ctcgcccttg
    20521 ttgtagcgct tctactgcgg catggtgccc aaagacgaaa ttatcttcta atgcttcttc
    20581 attttgatca gaaactgact gatttcgtcc agtttgcttt ttatttgaat atctattttg
    20641 ttttttgtct tcaaatccac gtttcgattg atgtttgttt gttctttctt tacccatttt
    20701 tttcaccaac tttctgaata caccagcgga tcaattcttc catccgctcg gtttgttttg
    20761 tcaaatgcag atagcccatg agcgcttcga accctgttgc gacccgatac gtcgtaacat
    20821 cagcattttt tgcgctcgta tggcttttag catttcttcc tctgcgataa taaagttctt
    20881 cttcttctgt caaaagatgt tcttccagca tttgctgaat caaaaaggct tgtgcattcg
    20941 cagaaacata attcgttgcc gcacgatgca gcttattggg ttttgtttct ccttgatcaa
    21001 tcaggtggtc acgaacatag atttcgtaaa tcgcatctcc aacataagcc aaggctaaac
    21061 cgttcaattg tgtgtaatct ctcatgcttc tcttctccat cgggttcctt gcggtgtatc
    21121 ttctaataag atcccttttt ctttcaacaa atcacgaatt tcgtcgcttc ggacaaaatc
    21181 acgattgctt cttgcttgat ttcgttcttc gatcaattgt tcaatctctt catctaataa
    21241 ctcttctgct gtaaataaaa taccgaagat cgaaagccat tgcgagaata aagcttctcc
    21301 ttgtgccaag acagcggcag aaactttcgg ctggtctaga taagtgttca gccacttagc
    21361 aagctcatag accactgtga tcccatttgc tgcattaaaa tcatcgtcca tttcttcgat
    21421 gaatcgctgt tctaaatttt ctaacaaagc gattttatcc gcatcatctg ccaaattctc
    21481 tgaggcagtt tcttggcgga atttcaaatt gtcaaacgta ttcttcagtc gttgtaaatt
    21541 ggtggctgct tctttcatcg tcgtttcact gtagcggatc ggacgacggt attgtgtggt
    21601 cgccatgaag aaacgcaaga cttgtggatc aacgtttttg atcatttcat gtacagtgat
    21661 gaaattccct agagatttac tcattttttc atcgtcttcg ccaatcgtta catagccatt
    21721 gtgcatccag taattagcga attttttccc tgttttcgct tcactttgtg cgatttcatt
    21781 ttcatgatga ggaaattcta aatcttgccc gccaccatgg atatcgatcg tttctcctaa
    21841 atgttttgtt gccataacgg aacattcaat atgccaaccg ggacgtcctt tgccccatgg
    21901 tgaatcccaa gaaatctcgc caggttttgc ttgtttccat aaagcgaaat ctaatgggtc
    21961 ttctttttgc tgctgttctt cccctgtacg ctgacttgct ccgacttcca gttcatcgat
    22021 ggattggtca cttaatttcc cataattttc aaatttacgg gtacggtaat acacatcacc
    22081 tgaagattca taagcatagc ctttttcaac caacacttga ataaatgcta aaatatctgg
    22141 catatgatcc atgactcttg gatgcagtgt agcaggttcg acattcaacg ctttcgtatc
    22201 ttcttcaaat gctcggatga aacgatcagc aacttctggt gctgtaatac ctaattcgtt
    22261 tgctgtacgg atgattttat cgtctacatc cgtgaaattc gatacgaaat cgactttata
    22321 gcctcgatat tcaaaatagc gtcgaattgt atcgaaagca atggtgctac gagcatttcc
    22381 gatatggata taattataca cagtcggtcc gcagacatac ataccgacat gtcccttttg
    22441 tatagggatg aattcttctt tttttctggt caatgtattg tagattttta tcattctgat
    22501 ctcctcactt tttctccttt gattcgaacg acttttgctg gtactcctac cgctgtagcg
    22561 tcgtctggaa catctttcaa cacaacggca cctgcaccga ttttggcgtt ttttccgatc
    22621 gttaccggtc ctaatatctg cgcattcgca gagagcatgg ctccttgttt aacagttggg
    22681 tgtcttttac ccgtttcttt tcctgtgccg cctaatgtta cgccatggaa taaaatcaca
    22741 tcgtcctcga tttctgcggt ttcgccaatg acgattccca ttccatggtc gatgaacacg
    22801 ccagttccaa tcgttgctcc tggatggatc tcaacaccag ttaggaaacg ccagaattga
    22861 gcattgatct tcgccaataa aaagagccgg tgctgatata aaaaatgaga gaaacgatgc
    22921 caaaacaagg catgaaaacc agggtatgtc aaaacgatct cagctgtcga acgtgcagcc
    22981 ggatcgtttc tctttgctgc atcaatcgct cgggtaaacc aactcatgac tttccactcc
    23041 tttcgtaaga attttatgta ggaaatcatc ttcttataaa gaaaagaagc gccttttaaa
    23101 taagtttcct tatcaaaaga cgctttccgc gtggttccac ttttcttcat aatcaatgat
    23161 tatctctgac agctaacgcc tgttatacgt tcttggctac tttgtttcct ataaaacagt
    23221 ttttcctgtt ttctgtgttt cgccaagacc actcccagag gcatttcggc aaccattcat
    23281 tgataccttg caccacccgg tatctctctt gtaatgatca gctgcttact tttttctgtt
    23341 catcgtgttt agttcagtac ttgatttaaa tgtgctaatg atttttcacg accaagcaat
    23401 tcgatcgttt caggcaattc tggaccatgc atttgtcctg atacagctac acggattggc
    23461 atgaaaagat tttttccttt tacgccagtt tctttttgaa cagctttgat ggcagctttg
    23521 acagttggag catctactac ttccatttgt tctagctgtg ctttgaatgc gtggagaaca
    23581 gtaggaactg tttcaccagc aagtacttct tttgctgctt cattcaatac aggatgttca
    23641 ttgaaaaata gcgtagacag atcaacgatt tctgcagcat agctcatttg aggttgatac
    23701 aggctaacga tcttcttcac ccattcgatt gtcttttcat ctgggttttc tgctacacgt
    23761 ccatcagcga ttagataagg cagacacatg tcagtaaatg catctagatc cattgctttg
    23821 atgtaatggt tgtttaccca ttgcagtttt ttcgcatcga aagctgcggg tgatttactt
    23881 aggcgttttg gatcgaacat tttgatcaat tcttggtgtt cgaagatttc gtcttcgccc
    23941 acaggtgacc agcccaataa tgcaataaag ttgaacattg catctggtag atagcctaat
    24001 tcgcgatatt gttcgataaa ttgcaagatt gattcgtcac gtttgcttag ttttttacct
    24061 gtttcactgt tgatgatcaa tgtcatatgt ccgaaggttg gtggtgtcca gccaaatgct
    24121 tcatagatca tcagttgttt tggtgtattg gcaatatggt catctccacg taaaacgtga
    24181 gtgattttca tcaaatgatc atcgaccgca acagcaaaat tataagttgg cataccatcg
    24241 cgtttttgga tgacaaagtc gccaccgata ttatcggatt cgaacgaaat agcgcctttc
    24301 accatatctg taaatgcgta ttctgtattg cgaggcacac ggaaacgtac aacagattct
    24361 agaccttgtg cttctttttc tgcttgttct tcaggtgtca agttcgcaca tgttcctgcg
    24421 taatgaggca tttctcctct tgcgcgttgt gcttcccgtt ctgcttctaa ttcttcttct
    24481 gtgcaatagc atttgtatgc tcgattgcta gcaagcagct gatcaatcag cggttgatag
    24541 atttctttac gttcagattg acggtaaggg ccgtattctc caggattttc aggagattca
    24601 tcccaattca ttcccaacca agcaaggttt tccaactggc ttttttcgcc atcttcgata
    24661 ttacgttttt ggtcagtatc ctcaatacga ataataaagt ctccatcatt gtggcgggca
    24721 aacaaataat tgaataatgc agttcttgca tttccaatat gcaggtgccc agttggactt
    24781 ggtgcatagc gtacgcgtac tttcgtcatt ttcttcctct tctttcaaac tagactttga
    24841 ctaacagcaa aaattccgtc agttacggct tctcaaatat catttattta aagccactca
    24901 gcacaaattt taccttttct aaggtaaata gtaaagccaa ctggttgaat ttccttacca
    24961 gttggctgaa tttctttttt attttgattg tttagtcttt ttctctgtct cttttttatt
    25021 gtcgattcct cgtcctgcat gtgcaggttt agcaaagatc atgcgtccag cagctgtttg
    25081 aagggcactt gttacaacga cttcgatacg ttcattcatg taatgctgtc catcttctac
    25141 gacaaccatc gtgccgtcat caaggtaggc tactccttgt tggcgttctg tcccggcttt
    25201 tactactaga acattcatcg tttcaccagg gatgacgact ggttttacgg catttgccaa
    25261 cgcattgata ttaagaacag gtacattttg gaattcagag actttattca agttatagtc
    25321 attggttaca atgacaccat ctaaaagctt agccaatttg atcaatttgc tgtctacttc
    25381 actgatatct tcaaagtctc catcgtacat ttcgacagat ataccctctt ctttttgcaa
    25441 ggcatttagg atatctaatc cgcgacgacc acgcacacgt ttcaaactat cccctgagtc
    25501 cgcgatgtat tgtagttcgt aaagaacaaa attagggatc atcaaggttc cttctaagaa
    25561 tccagtcttt gtaatatcat aaatccgacc gtcaatgatc acgcttgtat ccaagatttt
    25621 atatttatgg aaattttctc ctgattttcg ttctaacagt tgactctcaa cttgactagc
    25681 tgtttcttgc tcgatttttt tgcttcgtga accaaaaact ttcttccatt cttcgattcg
    25741 tgtcgttccc attctcaatc ccagatagcc taagatgatc atcagtaaaa tcggcaatac
    25801 actattgacg aatggaattt ccatattgta aagaggggtc gagatcacaa cacctaaagt
    25861 caatccgatg atcgcaccga tcgcaccgaa gagaagataa gtcaagctga cctcacttag
    25921 tttttgttct acttgtttga taatagctga gatatgtttg gccaacagca aagataataa
    25981 aaagaaaata agtgcgccta acaggctgtt ggtaaatgta ttattcagcc atccgtttga
    26041 ctgttgaccg attgcctgcc aagctgaggg taaaaatgaa atacccaagc ttgcgccgac
    26101 aatgatcatc agcaaagtaa tgacacgttt ttgcatcgtt tttcctccgt ttcgacaatc
    26161 atttattagt tagcggaaca cacgcttcaa cgtttcagcc aaagttgcta ctccaactat
    26221 ctcgattcct tttggcgctt cccagttacc taagttgttt ttaggtaggt atacttttgt
    26281 gaagccgagt ttttggactt ctttgactcg ttgttcaata tgactgacac gtctgatttc
    26341 tccagtcagt ccaatttcac cgatgaaaca ttccgttggc tgtgttcctt tttctttata
    26401 gcttgaagca atactgacag cgatagctaa atcaatcgct ggttcattta attttacccc
    26461 acctgcagct tttaagtaag catcttgatt ctgaagaagc aacccagctc ttttttccag
    26521 cactgccatg atcagagata cccgattgaa atcaagacct gttgttgttc gtttggcatt
    26581 tccgaacata gtaggtgtga ctaatgcttg gatctctacc aaaattggac gtgtcccttc
    26641 cattgccaca acgatagcag agccagttgc accgtctagg cgttcttcta agaaaatctg
    26701 tgaaggatta gcgacttctt ccaatccatg ctcatgcatt tcaaaaatcc caatctcatt
    26761 cgttgagcca aatcggtttt ttactgctcg caaaatccga aaactatgat gttgttcacc
    26821 ttcaaaatat aacaccgtat ccaccatatg ttctaacatt cttgggccgg cgatcgagcc
    26881 ttctttggta acatgaccga cgataaaaat ggctatccca tttgttttag cggttttgag
    26941 tagttctgca gtggtttctc ttacttggct gacacttcct gccacacttg tgatgtctgg
    27001 ctgcgtcatc gtttggatgg aatcaatgat aacataatca ggggagatat gttcgatggc
    27061 acgactgatt tcgttcatat ctgtttcagc gtatagataa aattctgtgt tgatacttcc
    27121 gaggcgttcc gcgcgtaatt ttatctgctc tgcgctttct tcgccggata catataagac
    27181 ttttccgcct atggctgcta attgctgaga aacttgtaac agcaaagtgg atttcccgat
    27241 acctggatcg ccaccaatca agaccataga ccctggcacc acacctccgc ctaacacacg
    27301 gttcagttct tctaattttg ttttgatccg aggttctttt ttagggacga cgtctgcgat
    27361 ttttgttggt tttgcttttt ctcctgtcaa acttgtccga gttctgcggt ccgttgtatc
    27421 ttgttcgatt tcttcgacca tcgtattcca ttttccgcaa tttgggcagc gtcccaagaa
    27481 cttaggcgac acatacccgc aattttggca aacaaattgt acttttgatt tttttgccat
    27541 tgtaccgcct ccttttatcc taatcatgag acattaaatc aagcttaagt atctttaacg
    27601 ttttatttac tggaagaacc aaatccgcct tgacgttgag cagtcgtttg atcttgatct
    27661 gcttttaaaa acggcaaaaa gatcccttgg ccgatacgct cgcctttttt gatcactaca
    27721 tccttgaaac caaagttggt gaattggaac atgatatggc cttcatttgc ctcattattg
    27781 taataatcac tgtcaatcac acctactccg tttgtcaaca ctaaaaaccg cttcaacgga
    27841 ttgcttgaac ggttagcaag ctgcaaaaat tcatcttctc ccatgtaagc tttgattcca
    27901 gtaggcacga gggttggttt caattgtttt tcattttctg atacatcttt tttcaatcca
    27961 tctgcgactg tttccaaccc ctttttccag cctgctttcc agatacttgg aatcacgacc
    28021 tcttctgctg cttcaaaatc atagccagca gcatgatgtg tagcgcgctg cggtagattg
    28081 atattttgat ctttatattt agaaataact tcaaatcctc tttttctcac cggttttcct
    28141 ccctaaataa actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa
    28201 ttcttcaaga aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac
    28261 tgcaagttgt acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata
    28321 gaacgaaatt aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat
    28381 gcaaaacgaa ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc
    28441 aatcttgcgg caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag
    28501 gaagtgatct tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca
    28561 gaacgtccga ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg
    28621 atgaatatct ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg
    28681 acaagagacg tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag
    28741 cttttgatta tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa
    28801 ttagatcatc tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt
    28861 gtcgcggcag accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg
    28921 actacaaatc ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg
    28981 gcacaagcag gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa
    29041 gccgcttcta ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg
    29101 gcaattattt ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg
    29161 aaatgactga cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac
    29221 ttatgcctac aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga
    29281 atcaacaagc gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata
    29341 tcagtgaaac gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta
    29401 cagagatcga aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga
    29461 cgaacaccga cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg atcggcatca
    29521 tttacgaatc tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga
    29581 atgccgttat ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat
    29641 tattgcagca agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg
    29701 atacttctca tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga
    29761 ttcctcgagg cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca
    29821 ttgaaacagg tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg
    29881 cagtgaacat catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa
    29941 cattgctgat ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg
    30001 tagaacacgg ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg
    30061 ctttagcaga agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag
    30121 tggtcgattc tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag
    30181 aaacgatcgt gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg
    30241 cggctgtcta tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg
    30301 cagagattgg tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat
    30361 taacttccac aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt
    30421 gaaacattat tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg
    30481 tcaatatgtt tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg
    30541 cttttctggg agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga
    30601 gtcaacggga aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac
    30661 gcttgcacat tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt
    30721 ttttcgatca ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat
    30781 cttataaata tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg
    30841 tacgataagt agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg
    30901 acaaagattt tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg
    30961 gtaaatctgt ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat
    31021 aacgaagcag ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt
    31081 cagccagtag ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt
    31141 ttctagtttc accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca
    31201 accgatcatc tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat
    31261 attcttccag catctattat ccccccattc tttttcttaa atgaatttta gcttacctac
    31321 tgaaaatttt ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca
    31381 catccccttt ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat
    31441 attcaacaaa catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga
    31501 cagtctcaca acctcttggt aaacggcgtt caatcagctg accctcggta ttagctcaaa
    31561 aaacggtaaa atatgagaag ctatttttcc gttttgtttc tctaatactt aggataaaac
    31621 gctgatttta caacctctgt taaacggtgt tcaatcagct gtccttcggt attagctcaa
    31681 aaaacggcaa aatatgagga actatttttc cgtttgtttc tctaatactc aggacaaaac
    31741 gctgatttta caacctcctg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc
    31801 caaccaaacg aaaaatatga gaagctattt ttgtttggct gttcttatga cttgatgcag
    31861 gacagctttt tcacaacctc tgatttctta ttcttcttca tttgtagcaa aataattacg
    31921 gtgataaatg atacaacata cactgataag aacaatggca cctgcaaaat aaaacgcata
    31981 gctttcacca atatgttcac caatcacacc tgcaataaat ggtgctaaag ctccaccgaa
    32041 aaagcggaca aaactatagc tggacgaagc aacgtttcgt tgcagacctg gtatttccat
    32101 agcaatcgtc gtcaacagtg tattgataat tccttggaaa aatcctgcta acacaatgct
    32161 gatcggtaca aggactggca tagaagttcc agctcctaat aacagcaaac aaataatgaa
    32221 taagacaaat gctgctaaaa ttgtccgata agtcgtaaac ttccgctcaa gagccggtgc
    32281 aacgaaaatc gaagcaatag ccagaagcac accccagcca aaaaatacaa aaccgacttc
    32341 taattcagaa tagccaatca ataaaaatgg cgcatacgca agtaaagtaa agaatccgaa
    32401 attatataac aatgcgatta gtccaactga acgaagttta tgattgctta acgctttgac
    32461 accttcccaa aggcctactt ttctctttgg tttctcagtt tcaggtaaaa gtaccgctag
    32521 ggctataaaa ccgcaaaaca tcaaaaatgc taccccaaaa aatggataac gccatgaacc
    32581 actgcccaag accccaccga ctagagggcc aacagccatt ccgcagccaa gtgctgcttc
    32641 atacatcatg attgcttttt ctgtgttacc aactaaaata ctgacgatcg ttgctaaagc
    32701 agtagagaca aataaagcat tcccgacacc ccagccggca cgtaacccaa tcagcattcc
    32761 gatattccca gataatccgc caagaccagc gaatacaacg atgataaaca atcctgtcat
    32821 caatgttttt ttcaaaccga tccgactaga taagaaacct gtaaatagca tcaccacacc
    32881 agtgactaac atgtaactag taaataacag ggtcgtttca gctggcgtag cgtttaactg
    32941 tgcagcaatc gtttttaaga ttgggtctac tagacccact cccataaaag cgatcataca
    33001 ggtagctgct acaactagcg ctgaactttt cccctttgtt tcttcatttg tttgttcttg
    33061 tatatctatt tgattctcca atatttccat ccctttctct ctattagcat catacatgat
    33121 ttattttaca tgtcaatatg cacatgtaaa acgtggaaac tttactgtga tataataaag
    33181 aaaataagac gagaggtagg atttttatga atactttagc ctatatatta ttatgcgtgc
    33241 ttttatccaa accacgttct gggtatgaat tgaagcagct gatcacaatt ttttgggaag
    33301 cccatcacag tcagatctat acaacgcttg ccaagttaga ccagcagggt tatgtgaccg
    33361 ttttggacaa agacgagcac tctcaaaaaa agatctacca cttgactgat gaaggacgtg
    33421 ctcttgtaga ggaatggatc aaagaggaaa ctcccgctcc tacgcaaaaa gatgaatttc
    33481 tagcaaaaat ctatgctttt tcgacgctcg ataaaacaac agctactgga ctgctattca
    33541 ctagagaaca acagctgaac aaaatcatgc aaaaaaatca aaaaaaattg aatgacctct
    33601 cgctagacaa gaaacaagaa tttggacgtt atgttgtgat ccagcgcaga attatgcttt
    33661 gtcagcagga actccaatgg tgtcagcaag taagagaaca aatgcagcag ttttttgagt
    33721 aagggataaa cagaaccttt ctagaaatat ctattgaaga gcaaattatc ttcaataaaa
    33781 gagcaataca gttggaaaag aaaaagcgat ccgaacgaca ggccaaaacc tatcgttcgg
    33841 atcactattt ttctagaact gcattcaaga gactatgaca aaaagcatgc caaggccttt
    33901 tttcaaataa cagtgttcca tcattcatta ctttgaattg cttttagcac acttcttgaa
    33961 agcgggccaa cgatgatcaa ctgcaacggt aaagcggcaa tcacattcat tttccaagca
    34021 ttcatataag cagagaataa ttgatcaggt attccttgtt ccatgatcac accgaataaa
    34081 gacatacatg tcaccattcc taaaaccatc aagcatgaaa tagttaagat catttggacc
    34141 ttgctttctg gatttactgg tagtttaaag gcaatttttt tcgccagcac accgatgatg
    34201 aagacatcca aaatgaaagc caccacgaat ccaggcacta gaccaatcaa caaacttgat
    34261 aaatgaagat cttgatgcag ccataaatta tacgcgctca tccccaagac catcaaaaaa
    34321 cacatcattg ttgtaaaaat tattccttct tttttatttg taggcaatct gtaccactcc
    34381 tttaaatttg tcacgaaaat catcgtagca catttaaaaa tttctcgtaa gtactttttt
    34441 tattttctgc ccttttattt cataaacttc atcagccgta cgctcgacaa agtaagaatc
    34501 atgagaagta aacaaaatcg ttcccggata tatttgcatt agcttctcta aagcttcgat
    34561 cgttgccata tcaataaaat tagtcggttc atctaacagc aacacattac ttggcttagt
    34621 aaaaagcaaa gcaatcgtca gacgggtcgc ttctccccca cttaatgtac ataacggttt
    34681 cgtcaaagtt tcctgagcaa accctaagtg attcagcaaa ctacggacaa gcgattctga
    34741 aaactctgtt tgtctcatga caaaacgaat gatggattct tcagatgtca tttgataacc
    34801 cttttgctga tatacttgaa agcttacttt cggagagaga aggattcctt ctccttgttt
    34861 gcggatatgt tctaataagg ttgtcttacc tgaaccattt tcaccgacaa tcgcgatttt
    34921 tttacctaaa gaaaactgaa aatcaccatt taccagtaat gttctcccac ttctttccaa
    34981 ttggacattt tgtcccatga tgggatagcg gctgtggagt tcaagagatt ttggtactgg
    35041 aaaacggatt tgtttcatct gctgcggctt ggttgtttct cctatttttt ggagtcttct
    35101 ctctaatgtt ttcgcattct tttggatggc tttttgtaca ctgtcttttt gtttagagga
    35161 agacaaccga tccagtctga tttgttgctt tttcttttta ctggacactt tttccaactt
    35221 ttccgcttgt tttttctttt cttggatcga tttttccaat cgttttttct ctttcatgat
    35281 cgactctgct tctcgtagct gagtcttttt ctccaattcc ttttgagtga gataggcact
    35341 ataattcccc gaaaactctc ggatttctcc gtcttttact tcccagattt tcgatgctag
    35401 ctcatctaga aaatagcgat catgactaac aaaaaccagc gtaccataat aataacgaag
    35461 ctcttcaatc agtcgatcaa tactttttct atctagatgg gttgttggct cgtctagaag
    35521 catcccttgt tcataagaag aaagaagacg tatcaactcg atctttcgtt cttcccctcc
    35581 gctccatttc cgctctgaag tttcttccag tcttaactgg aaatgactgg ctaattccaa
    35641 atttaggtcc tttgcttcag cgagataggt taattgaggc aggtagttga aggtgatctc
    35701 tgtttggatt ttcccatgat cgggaaaaag ttcaccggct attagtttga gcaaagtcga
    35761 tttaccgaca ccattttctc ctatgatccc tatacgatct tgctgataga cacgaagaga
    35821 atcaatagat agttctagct ggttgccaaa gctgacttgt agatttgtta tatttactgc
    35881 taaattttcc atatgtttta cactcctatt tcatgtaaaa caacaattat cgatactaaa
    35941 aaagagcaga aaaatctgtt tgcaaaaaaa ctgcatcaca tcttttccta ctctgaacga
    36001 aataactatg actaaatcac caaatctgtc atagctttca cctattctac tgtcgttcga
    36061 aaaaatggac atactccatc catgaaattg ttgtttatac atgtcttttg aatttattta
    36121 gttgttattc aaaagttcga aacgtaattt catcgatgca gtcatttttc cgacctcctt
    36181 ttttattagg ttaccacaca cgattcaaaa aaagcaatag ctactgaata gtaactgaaa
    36241 accttttatt cgaacatagt gatcttttct ggtgctaaac gaacagactc gtaaccttct
    36301 tctaccgctt ttcctattga taaaataata actggaacat aacggtcttt atccaaacca
    36361 aatgcctctg ccaactgatc tgcttcgaat ccaccaattg gatttgtttc atagccatga
    36421 tcacgcgcaa ctaacatgaa ttgcatagcc gctaagctag aatcgacttt gactacatcg
    36481 ttcatttctt ctcttgaaaa actcttgtaa taaggaatga tcgcagctaa ttgttgatct
    36541 ctgacttctt ttggcatctt ccctgattcg taagcttggt tgtagatttc ttcgccatat
    36601 tcgtaacatt gcatatcgcc aaaaatcatg accattgcag aagaagtatc attttgtctc
    36661 gtattgaaac ggatcaaagg acgcaagatt tcttttgctt cctcgctttc agcgaccaca
    36721 aagcgccatg gtttcatatt gacagaagaa ggtgcagtcg tcgcttcttt gatcatttcc
    36781 agcatttctt cgtgtgggat tttgtatgtt tcatcatata cgcgaacgga tcttctacca
    36841 gtcataattg ttgaaaaatc atttttttgt tccattatta ttaacctctt tcgtttctgt
    36901 tgttatattg caaagttggg tattcaattt atctaaaagt gttagaaatt gtttcatttc
    36961 tacatcgctg aaagccgggg atagcacgga acctgtatct ttatggtcca tttcacaatg
    37021 cttgattttc ttctgggctt tttctgttag cgctacatac acttcccggt tgttctgagg
    37081 atttctctga cgcgaaacat agccatttgc ttccaatacc ttcagatgac gagtaactgc
    37141 tccttgatca atctgcaact tttcttgcaa actgctttgt agcaatggat tttcttcttt
    37201 cagtctcatc atgatctcgt atctagtaag acttataccg atattttctt caaagatctg
    37261 gacgattttt tgatcggcta atttgatttg atataaaagg tgactaatct cttctaaaga
    37321 cataatttcc tcctttctaa taattgactc gtcaacgatt gacttgtcaa taatagaata
    37381 aaaaaagaaa aaagtcaaag aatcagctgt gtaataaaaa aaacgctctc aatagaaaag
    37441 tatagagaaa agaaattttt tgtagaatga acggaaaaaa tgataaagaa agcacaacgc
    37501 taaaaccaag attattgatt cttatcaaac tatagaagat agctcttctg cagtagataa
    37561 ttttacaccg ccaaaaattt ccgaaatcgc cttttgcaag aatcccgtcg aatattagaa
    37621 attggatcag tatgcttaaa cagtaataaa aaagttatga caagatgttg tcacaacctt
    37681 ttttgagtaa acggtgttca aaaacttgca tctgcggtaa taagttcaac caaacaaaaa
    37741 acatgaaaat ctgtttttgt ttggctgttc ttactacttg atgcagggca gctttttcac
    37801 tacctctttt taataaccat aaaatcctat tccttcatat ctccagccca tcggtgttag
    37861 ccaatctctt tccttagcac tcaaggtata gtgatgggta ccagtttttt catttggatt
    37921 aaatagtcga taaattggct tggaaccacc agaataaaaa cttagacctt cataacgcca
    37981 accgattttt gttaaagagt tgatctcaga ttgtgccatt gtgtaatgat ggtctccact
    38041 gtaaggattg tataaacggt agacaggttg tccgctgttt ggtgcttgcc aagcaatccc
    38101 ttcatattcc cagcctacag ttggcagata atttttttct ccggcattca atgtatataa
    38161 atgttctcct gtatgacggt tatacagacg atacatcgga gaagttgaag ttggtgctgt
    38221 atcatactgt gtcagattat aggtttcgat tacattgttc aatgtagaac cataattagg
    38281 cgcagtggca taacgacctg ttagccaagc cgtagcatct ttgtaagagt tcgtattgct
    38341 tttccatgca ccggaatagt agtaaacgcc tggctgaaaa gaagtcgtct tcaataccgt
    38401 tacgttatct tgcaatgatt ctttgtacga aggatatttt ctaaaagcgg cattcaccgt
    38461 aatccattgc ccattcacat attccgaagt aggcatcgta acagattggc cattatagct
    38521 tcctttaatc ccaaatagat tgtaattagg ggcagaagcc aaagtactgt tcccccagcc
    38581 actttcaacg atggcttgag caatcatgac tgatgcatat aagtcattgg cacttgccaa
    38641 actttgtgca gttgaggcaa tactatcaat gaacgcttgt tgagaattag cagatgagcg
    38701 agcataaaaa gcatagccag tatagttttc gaattcagac aaatctgctt gatgggtatg
    38761 tgtcaatgtt tgcccatctg tcaattggtt cgtgatttct tctgcattct ctggattgtt
    38821 ctgagcttct gctgatgtac tgtcttcggt ttgtgtttgt tctgttgttt cagttgttgg
    38881 ttcttcggtt gaaattgtat tttctgagac agtagctgac tgcaaagtac tttctgtatc
    38941 gttttcaatt cctgtttctg aagaactcga tgtctgagtt gcgacagttt ctgtcgattc
    39001 ttcagctagc actgttgtgt tggcaactgc tagatttcct aaaagaataa cgctaaataa
    39061 taaattcttt gctttcatct tttttctcct ttgaattatg aaacttttcc taaataacag
    39121 tgtaactaaa aaagtaaaag caagtcaatg tccatattta aataacattt gtttcttttt
    39181 aataaatttg ctttttctgc ataaaaagac gtaaatgaag aatattcttc tccattcacg
    39241 ccttaaattg atggtcgttt gaataactat ttaacaagta attctctctg gatattatcc
    39301 gttatggatt atacttcctg tgtcgtcgga cggataatca tgttgctgac agacatccgg
    39361 tctggcgttt ctatcgcaaa tgctacagca gaagctatat cttcagatgt taatccccat
    39421 tctttttgcg cttcatgaag ttcaagagca gcctttttgt cgctgatggt ttgatataat
    39481 tcagtttgaa cagctcctgg agagatgatc gtagatttga tgttattttg ccgttgctct
    39541 tgacgtaatc cttccatgat tgcacggaca gcaaattttg ttccgcaata aacggcggaa
    39601 tcaggataaa caacatgtcc cgccacagaa tcagttgaaa tgatatgccc agatttttgt
    39661 ttttccatga ttggaagtac ggcggaaatt ccatttaaca cacccatgat gttgatatca
    39721 agcatatttt gccattcatc acggtgacct tcaactaatg gagcggttgg catgattcct
    39781 gcattattgt acaatacatc gattcgaccg aacttttcca tcgctaaatc gataactgct
    39841 tggacttgtg caaaatctct tacatctgct gtttgaatat atagctcagc ttctggtaaa
    39901 gattctttta ttgcttttag gcgatcttct cggcgtgcag ctataactag tttagcccct
    39961 ttttctgcta atagcttcgt tgttgcttca ccgattccgc ttgaagcgcc catgatgaca
    40021 atcacttttt ctgataaatc attcattcta ttctcctcct gttttgaaaa atctcttgct
    40081 tatactcact atctcttatt tcatcttact gttcaaataa tccgttcgac aggtttgacg
    40141 attttggcag ggattcctgc aacgatggta ttgtctggga catctttcgt aacagttgca
    40201 ttcgctgcaa caatggcatt ctcgccaatc gttacgcctg gcagaatagt tgctcctgct
    40261 ccgatccaag catttttctt gatgtggatt ggctttactg acaagcctct tcgttctttt
    40321 ggatcggtta gatggttcac cgtgatgagg ttgacacgag gaccaatcaa tactgaatct
    40381 tctaaacaga tgccacctaa gtcaacgaac atcacttgac gattgataaa gacatttttc
    40441 cctatactaa tatgtttgcc gaaatctgta taaaaaggca gcagaacagt caccgtatca
    40501 tctactttgt cactaatgat attttctagc cgtttgcgga tatctgctgg ttcatggtac
    40561 ccagtattca gcgctgctaa aggctcttcg ttttccgctt ggatctgatg gatcacttca
    40621 aagagcggat ccgttactcg aatctcttta ttgacgattt gtttttcgat gaggcttgtc
    40681 attttgctcc ctccttttat ccttacttta taacggacgg cttatgatgt ctaatactta
    40741 taaaacatac ttaactattc cttttaacta tacttagtgg tatgcttttt taaaaacagg
    40801 agggaaaaaa tggatattcg tatactgacg tattttttag cagtagctag agaaaaaaat
    40861 atcagccgag cagcaaaatc tctgcatatc acgcaaccta ctctctcaaa acaactgaaa
    40921 gatttagaag aagaacttgg tgtgacactt ttcactcgag gctcccgaga gatacagttg
    40981 acggattctg gtcgatatct ttattcaaaa ggaaaagaaa tcttgtcgtt ggtagaaaaa
    41041 acggcgaaca atttaacaaa tgacgcaatc atctctggag aaatctacat tggcggaggc
    41101 gaaactaaag caatctcttt catttctgat agccttcatt cgttgatcaa ggcgcatcct
    41161 gatatccagt ttcatttata tagcgggaat gcagatgata tcatagacaa actggatagc
    41221 ggattacttg attttggact cgtaatcgaa ccgacaaaca aacaaaaata ccagtcattt
    41281 cgactgccag cccaagatcg ttggggactt ttgatacatc ggaatcatcc tttagcaaag
    41341 caaacagtca tccatccgca ggaactgaaa actgttcctt tgatcatttc ccggcaaacg
    41401 tcagtggaca gtcaactagc tgaatggcta ggtttcagca tagatcagtt atcgatcgtc
    41461 ggaacatata acttattgta caatgcttct ttgatggtcg ataacgggac ttttggtgca
    41521 ttagctctag atgggatcat caatacttat gggacggaaa aagtattcat cccactcgat
    41581 ccgccattac aagcttcact gaatctcatt tggaaaaaag accagcctct ttctcctgct
    41641 gctcgtaaat ttttagatgt cctaacggag aaatacagtt aagtagagtt tttttccttc
    41701 gctttcagct ttttaacaac gaataagtta agcaaaagat ccaatgatta aataacataa
    41761 gtttcttaat ttgttccttc cttttatttc tgataattcc agtaaattac tataa
//
LOCUS       DAYYCW010000011        38440 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000011 DAYYCW010000000
VERSION     DAYYCW010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.011"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            266..586
                     /locus_tag="ACHAYA_000601"
     CDS             266..586
                     /locus_tag="ACHAYA_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM8604430.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            740..1645
                     /locus_tag="ACHAYA_000602"
     CDS             740..1645
                     /locus_tag="ACHAYA_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8604431.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1642..2415
                     /locus_tag="ACHAYA_000603"
     CDS             1642..2415
                     /locus_tag="ACHAYA_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8604432.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2596..4020)
                     /locus_tag="ACHAYA_000604"
     CDS             complement(2596..4020)
                     /locus_tag="ACHAYA_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM8604433.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4231..4677
                     /gene="nrdI"
                     /locus_tag="ACHAYA_000605"
     CDS             4231..4677
                     /gene="nrdI"
                     /locus_tag="ACHAYA_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8604434.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4742..6724)
                     /locus_tag="ACHAYA_000606"
     CDS             complement(4742..6724)
                     /locus_tag="ACHAYA_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8604435.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6738..7634)
                     /gene="murQ"
                     /locus_tag="ACHAYA_000607"
     CDS             complement(6738..7634)
                     /gene="murQ"
                     /locus_tag="ACHAYA_000607"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8604436.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7637..8698)
                     /locus_tag="ACHAYA_000608"
     CDS             complement(7637..8698)
                     /locus_tag="ACHAYA_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8604437.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8909..9436)
                     /locus_tag="ACHAYA_000609"
     CDS             complement(8909..9436)
                     /locus_tag="ACHAYA_000609"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8604438.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9503..11005)
                     /gene="eat(A)"
                     /locus_tag="ACHAYA_000610"
     CDS             complement(9503..11005)
                     /gene="eat(A)"
                     /locus_tag="ACHAYA_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8604439.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11263..12384)
                     /locus_tag="ACHAYA_000611"
     CDS             complement(11263..12384)
                     /locus_tag="ACHAYA_000611"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8604440.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12510..13451
                     /locus_tag="ACHAYA_000612"
     CDS             12510..13451
                     /locus_tag="ACHAYA_000612"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8604441.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13601..14497)
                     /locus_tag="ACHAYA_000613"
     CDS             complement(13601..14497)
                     /locus_tag="ACHAYA_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8604442.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14766..16106
                     /locus_tag="ACHAYA_000614"
     CDS             14766..16106
                     /locus_tag="ACHAYA_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8604443.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16324..16767
                     /locus_tag="ACHAYA_000615"
     CDS             16324..16767
                     /locus_tag="ACHAYA_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8604444.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17271..18689)
                     /gene="nhaC"
                     /locus_tag="ACHAYA_000616"
     CDS             complement(17271..18689)
                     /gene="nhaC"
                     /locus_tag="ACHAYA_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8604445.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19092..20315)
                     /locus_tag="ACHAYA_000617"
     CDS             complement(19092..20315)
                     /locus_tag="ACHAYA_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8604446.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20346..21185)
                     /locus_tag="ACHAYA_000618"
     CDS             complement(20346..21185)
                     /locus_tag="ACHAYA_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8604447.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21202..21882)
                     /locus_tag="ACHAYA_000619"
     CDS             complement(21202..21882)
                     /locus_tag="ACHAYA_000619"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8604448.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21884..22957)
                     /locus_tag="ACHAYA_000620"
     CDS             complement(21884..22957)
                     /locus_tag="ACHAYA_000620"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8604449.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23013..23251)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23294..23527)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23604..24275)
                     /locus_tag="ACHAYA_000621"
     CDS             complement(23604..24275)
                     /locus_tag="ACHAYA_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8604450.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24362..27010)
                     /gene="mgtA"
                     /locus_tag="ACHAYA_000622"
     CDS             complement(24362..27010)
                     /gene="mgtA"
                     /locus_tag="ACHAYA_000622"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM8604451.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27352..27526)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27699..28136
                     /gene="ndk"
                     /locus_tag="ACHAYA_000623"
     CDS             27699..28136
                     /gene="ndk"
                     /locus_tag="ACHAYA_000623"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM8604452.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28158..29024)
                     /locus_tag="ACHAYA_000624"
     CDS             complement(28158..29024)
                     /locus_tag="ACHAYA_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8604453.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29184..31277)
                     /locus_tag="ACHAYA_000625"
     CDS             complement(29184..31277)
                     /locus_tag="ACHAYA_000625"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8604454.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32026..32649)
                     /locus_tag="ACHAYA_000626"
     CDS             complement(32026..32649)
                     /locus_tag="ACHAYA_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604455.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32808..34205)
                     /locus_tag="ACHAYA_000627"
     CDS             complement(32808..34205)
                     /locus_tag="ACHAYA_000627"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8604456.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34237..35208)
                     /locus_tag="ACHAYA_000628"
     CDS             complement(34237..35208)
                     /locus_tag="ACHAYA_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8604457.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35582..36007
                     /locus_tag="ACHAYA_000629"
     CDS             35582..36007
                     /locus_tag="ACHAYA_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604458.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(36495..37196)
                     /locus_tag="ACHAYA_000630"
     CDS             complement(36495..37196)
                     /locus_tag="ACHAYA_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8604459.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(37212..37652)
                     /locus_tag="ACHAYA_000631"
     CDS             complement(37212..37652)
                     /locus_tag="ACHAYA_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8604460.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(37652..38227)
                     /locus_tag="ACHAYA_000632"
     CDS             complement(37652..38227)
                     /locus_tag="ACHAYA_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM8604461.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT        11123 a         8016 c         6694 g        12607 t
ORIGIN      
        1 ttaaatgatt aataaataac aacttctttc tatgaaattg caaagcattc atcttctttt
       61 ccgctaacga tatatccgtc agtgcttggt acactttact aaaaagtatg agattgccga
      121 tatctggata ggtgtgctat actacagaaa aagatataca atctgccacc gggtaggata
      181 aagaggatga tcattccatt aggtctttga agggatgatt ttcctctttt tgtgtatttt
      241 tttatagtta aggaggaatg taagtatggc atggatggaa ttaatcatag caggaatttt
      301 ggaagtattc tggtcaacga tgatgaaatg gagtgatggt ttttctaaaa tcaattattc
      361 tttttacaca gtgattggca tgattgctag tttttacttt ttgtcaaagg caatcaaaag
      421 tttgccgatg agtctctctt atcctatctg gacagggatt ggcgcagttg gttcggtcat
      481 aattggtgtg gtcttgtttc atgataagct gaatctttcc acttggttct ttgtggggat
      541 gctactcatc agtatcattg gcatcaagat tactagcggc cactagacac ttggaataag
      601 tactgatcag aaaaaataag cctttttatt taatattcaa tttttttatg aaaaaataag
      661 aaataagtcc aatgacctag cgacttctcc actcatcagg tgtatgcttg tactatctat
      721 agaaaaggag tgaatatttt tgaaaagtct ttcggtccaa gaattatcca agaactttgg
      781 tcacaaacaa gccctcagcg atgtcagttt tagcttatcc cctggcaaaa tcgttggatt
      841 agtaggtcct aatggcgctg gaaaaacaac gatcatgaaa gctattttag gactgatcca
      901 ttactcagat ggaacaatct gtattggtga aaatgagatt tctccctcta gtcataaagg
      961 gttggaacaa gtaggagcat tgatcgaata cccgggtatc tatccattct taacaggcta
     1021 tgatcatctg aagttattct ctgaaaccaa cgatgtatct gctatcgaca ctatcgtgaa
     1081 tcagctcaag atggaaaagt acattcacaa aaaagcgaaa tcctactcgt tgggaatgaa
     1141 acaaaaattg ggaattgctc ttgccttact gaaccatccg tctatcgtta ttttagatga
     1201 gccgatgaac ggtttggatc cacaagcaac aagagatgtg cgaaaaatca ttaccgactt
     1261 ggctactcag ggcacctctt tcttgatctc cagtcactta ctcagcgagc ttgaaaaaat
     1321 ggtcgatgag ctgatcctga tcaatcaagg aaagatcatc aaacaatgta caatggctga
     1381 attgaaccaa acagcgcaag atttcatcgt tatctctaca acaaatgatc tagctgctcg
     1441 aacgatcttg acagaagctg gatatacttt ggaagatcct gataaaatca agcttttaaa
     1501 agaaaacgaa gaattattag gaaccgtgat ccagctattg tctgcacaaa atatccatgt
     1561 gacagacgtc cagcatctga cgaatgattt ggaatcttct ctattggaaa ttctggattc
     1621 agtaaagggg gaagaagaaa aatgacattg cttgtaaaac aagaattatt taaattgatc
     1681 aagaaaaagt caactgctgt tttatctgtt ctattagttg ttttgctcat aggaacagca
     1741 cttctagcca aaaaatacac gacgatcatt gatccagttg aaatgactgc tcagcttttt
     1801 agtgccacat cttggattgt ctttatcatg attgcagctg caagcacgat catttcgatg
     1861 gaagcacaat atggtacatt gaaaaatctc ctttatcgaa agtattctcg aggagaaata
     1921 ttagtaagca aatggatcac attagttatt tattctgttt atttatacct tttagctatt
     1981 attgtcactg tgttgatgaa actgattctt ttcccttcga tttccttcac ggagaaggtt
     2041 tctactggtc aaacactgat ccaaagcttg ttcacttata ctttgggtag ttatatcggt
     2101 ttatggctga ttttaagttt agtgttgatg attgcttgct ttatcaatag ttccggcgca
     2161 tccatctcag caggaatcgt cttttatttt gcttcgtcaa tcatatctgg tatccttatt
     2221 gcactcatcc aaaaatggga gtggatcaaa tggaatccga tcagtatgtt gaatctgcaa
     2281 aaccaaattg gaaacgaaga gatcatgaag caattgactc atctttctac taatcaaatg
     2341 ctattcggta atttagctta catcgttttg ttcttagcat taggatatct cgtatttaag
     2401 cgaaaaaatg tatgatgact catcaaaaaa gaggctgtga aagagacgtt cagccttatt
     2461 gccgattggc tgtctcttaa acaccgttta ttctgaaaaa ggagctgtga caacatttgt
     2521 gtcacagctc ctttcctagt atgtttttga ataaacgaca ggctcacaac ctctctttat
     2581 acttctttta tacttctata cgtgaagcat cccagtggca aataaaacca ttgcaatcac
     2641 tccgaccatc gtcacaccta taagtaaaat cttatctaag tggatatgtt gtacaggctc
     2701 gttttgctcc ttacgtgctt tgaggtaaaa ataaatgcct ggcaagaaaa gaacaagaac
     2761 aagcattact tcattaaagg ctacgactag catagcccat acttgaaagg cacttgctaa
     2821 aagccctact aaaaattgga cccattcttt ccgctgataa gaaagtttca tttggtacaa
     2881 tcctaagaaa agccacgtga agaaaatagc tgctgtacca agtgtgtaag caaagttata
     2941 agcactgtca gtgaataata atgaaagtaa aaagacattc gtacataacg tcgtcaatac
     3001 gatagcataa gttggtgccc catttttgtt gagttttccc catgctttag gcaaaagtcc
     3061 gtcttcagcc atgtttccca ttgtttcaat cggtaagatc gtccatgaga gccaaacacc
     3121 aactaaagaa atgatcaaac cgccgttgat cacgactgct ccccatttac caaccatcat
     3181 ttcaaacaca tagcccatag caggctgcgc cttgatcgtt tgcagctgat tcatcgtcat
     3241 tgtcccatat ggaagcatgg aaaccaagac gtaaataata atcaagccga atatccctag
     3301 aatcgttgct ttcccaacat ctgattgttt ttttgctcgt gaacctacga cagaggctcc
     3361 ttcaatacca acaaataccc aaagcatcac catgatgcaa ctcaatactt gactggaaaa
     3421 cgggatcggc tgtccattcg cattcaatat aatgtcgcta gaaaagttat caatgaattg
     3481 ttggatcgaa aacgtgctga tagctactac aataaataaa aataatggca ctaatttaca
     3541 gaatgtgacg atagcattga cgaaactagc attttcgact ccccgattca ctagccatgc
     3601 atatccccat aaaaagactg tactcatcac gatcgatgcc agattttgcc caccttcaaa
     3661 tactgggaag aaatatccta atgcgctcat caacaatgtt gcaaaagcta cgttgcctaa
     3721 ccagcatgtg agccagtaac cccaaccgct gataaaacca gcgaaacggc caaaaccttc
     3781 ttctgcgtaa ctaaagatcc cgccttttaa atctggacgt tttttattga tattattgat
     3841 cgacaaggag agcatcaaca ttgctgttcc tacgagtacc caactgaaaa tagctgggcc
     3901 gccagccgat ccggagatgt ccgtcataat accaaatacg ccacctccaa tcgctgaacc
     3961 aattactaag gcagcaagtg gaattaaacc aattcctttt ttctgttgct ctgtatccat
     4021 gtaagcatcc tccaaaataa aaccgctttt atcaaaaagt ataacaaacc ttttttattt
     4081 ttacttaatt aataaaaact ttaatagaaa tataattcta ccggcttttt tctcattata
     4141 caagcaaagc gcctagtccc tttccaaatt acaccaccaa aaaacttaaa aaagatgcta
     4201 tactaaaagg aaagaattgg aggatgatcc atgaacattt tatatatatc gatttcagga
     4261 aatacgcgtg cctttgccaa acatctagca gaatatgctg aaaaaatgca tgcagaagat
     4321 cctgtcaatc ctgaagttac tttaaaagaa atccatgaaa atagtgattt tacgaaagaa
     4381 acagaaccat tttttacttt cgttcctact tatttagacg gcggaaatgg tttagataac
     4441 ggtgatacag aaatcttgac cgaaacgatg cgtgaatatc tggaacatga agacaatcat
     4501 aaactttgtc ttggtgtagt cggtagcgga aataaaaact tcaataatca gtattgtcta
     4561 acagccaaac aatatgcaca aacatttggc tttcctttct tggcagatta tgaattacgg
     4621 ggaacaccat ctgatgtgac acgaatttat cagattttag cagaaaacaa taaataataa
     4681 aaaagaagaa gctgaaaaat agatttcagt ttcttctttc gtttctattg tttctatcat
     4741 attaagcttt gacatccatc acaggtgctt ttgcttttgc tttacctgtt ttttccaaga
     4801 caaagttttt tacacgatcc atattggtta ccaagacgat catggatgtt tctttgcctg
     4861 ctgctttgat cgcttctaag tcagcttctg ctacaagcgt atcaggtgtc acttgctgcc
     4921 cttctgttac tttcacatca aatggcgtac cgcctaagtc aactgtgttg attcccatat
     4981 gaagcaagat ttctaaacca tttggcgttg tgatcccaat agcgtgtttc gttgggaaga
     5041 cagagctgac taccccagta actggagaat agatatgtcc gttttctggt tctacaaagt
     5101 agccttcacc catcatcttt tgtgcaaaaa ctggatcact tgctttatct aaagaaacaa
     5161 ctgttccgtc agcaactgat gagaggatag tcacactttc agctgaagtt tgaggtacta
     5221 ctttttcagg cacttcattt actgtggctg tttcagtcgc taattcttct ttttctaaac
     5281 ggtctttagg aataccgaag aagaatgtag caacgaagcc accagcataa gctgccaata
     5341 atccaaggat gtagcctaac catttaccgt cagagatcaa tgggatcaaa gcggctcctg
     5401 aaggtccaat agcgatagcc ccgacatttc cgatcattcc gatcactgcc ccgccgatac
     5461 ctccaccgat acaagcagta ataaatgggc gtcctagagg taatgttact ccgtaaatca
     5521 atggttcgcc gattcctagg atcccgactg gtaaagctcc tttgatcatt tctgttaatt
     5581 ctttatcttt tttgcagcga atccataatg ccaatgctgc acccacttgt cctgcaccag
     5641 ccatcgcaag aattggaagc aatagcgtac gaccagtttc atcaatcatt tgcacatgga
     5701 ttggagtcaa aatttgatgc agtccgaaca taaccatcgg caagaataat gtgcctagaa
     5761 cgaagccggc aaatgctcca ccaacgccaa gtacccagtt gatcccaccg accatgccat
     5821 cagaaatgaa acctgctaaa ggcatgatga agaaaatttc aatcagaccg atggcaatca
     5881 atgaaagagt tggtgtaacg atgatatcta ccgcatcagg tactacttta tgcagttttt
     5941 tctcaacgat tgacaatagc caaacagcga agatgacacc taggatccca ccttgtccag
     6001 ctgcaagagc ttcaccagta aataagtttt tcagtggtgc ttccggattc atccctgtca
     6061 gcatcacaac ggccccgata actccaccaa gagttggtgt tgcaccaaaa acttgtgcag
     6121 cattgatacc tgtatagata actaggtagc taaacatccc gtttttcaag atgttcatca
     6181 catcgatata ttgttgccat gttgctgcat caagtgttcc ggctgtgact aagtttgcta
     6241 aaactgcggc aatccctgcc acgatcccag taccgacaaa tgcaggaatc attggcacaa
     6301 agatattaga aatgtctttt aataccgatt tgattttaga tggtttttgt ttcgattttt
     6361 gtgccgcttt catttcttgt gcccgttcgt ttacacggtc ttttccagat gctgatgaat
     6421 ttgctgtagc tgtacttaca gaagaaagtt tttctcctaa tttcacgcct gccaaatcaa
     6481 ccatttgctg tgctactttg ttcactttcc caggtccgat gatgacttga agctgttcat
     6541 cttctactac accaagcact cctggaatcg cttttaattc agagacattt acaagatcat
     6601 cattgatgat cttcatacgc acacgagtca tgcagtggac aatgctttct acattgccca
     6661 tgccgcctgc ccctttataa atttgttttg ccagttcttc caaagtcagt tcgttttttg
     6721 ccatggtctt ttccccctca taaggtccct tttacaaatc cgtttgcttc agctaatttt
     6781 gcttgagctt cctctttatc actatttgtc aaaatcatga cgatagccag cttaacattt
     6841 tgatctgctt cttcaaagta cttttcagct gtttcgtaat cagcttctgt cgcttgcatg
     6901 atgattcgtt ttgagcgttc gattagtttt tcatttgttg gtttgacatc gaccatcaag
     6961 ttattgaata ctttacctat accaatcata ctgatagtag aaatcatatt caagataagt
     7021 ttttgtgctg ttcctgactt caatcgagta gagcctgtca aaaattctgg acctgcatcg
     7081 acttcgattg ggaaatccgc gtgtttactg atttcggctt gtttattgca agaaatcgtt
     7141 cctgtgacag caccgatcga ttttgcgtaa tctaatccac cgatcacata tggtgtacga
     7201 ccgctagctg cgataccaat aactatatca ttttttgtca aattcagctt gattagatct
     7261 tcttgcccta gctcataaga atcttcggcc ccttcgacag ctacagtcat tgcttcttct
     7321 ccaccagcaa ttagacctat gaccatttcc ggttctacac cgaatgtcgg tacgcattca
     7381 gcagcatcta acacacctag tcttccactt gttccggctc ccatataaat cagacgtcca
     7441 ccttttttaa aggactcaat cgtgttagca atcactggtt cgatacttgt cagttctttt
     7501 tcaaccgcag cggccacttt ctgatcttct ttgttcatta gttgaacggc ttgagaaata
     7561 ctcatctcat ctaatccaaa agtttcttgg tttctgcgtt ccgttgttag gttctctaaa
     7621 ttgattttgc tcatttttat cttccttctt ttcttataaa ttgatagttc ataccagctt
     7681 tgatttggtt gatcaatggt agctcttctt tgataacttt agctactaca ttcacttttt
     7741 catccgctgg caaatcatat ttggttaatt ggatctcacc catatagcgc aaatactttt
     7801 cgttatctaa agtaatagac cctttcatac gcgtagctgt atttcttgct gggataggcg
     7861 gaatctctcg aaaacgtgca tctacgcttc gtatgacatc tcttgcatcg tcttgccgat
     7921 tcgtatgctt ccctaatata tattcgtaaa attcttcatc tacaggttcc gtatggagca
     7981 agactgtctg ttctttttgg aaagaagcaa attgctcctg aacgttttct gacagacctg
     8041 catctccgat gtaaacaata tccgtgttac ttgctaatag atctagagcg cctgcaagag
     8101 gatggatccc tcgatgttct tccaaagttg gcaatccttg atatagtggg cctctcaact
     8161 ctgtatctcc tggtacaaaa ccttggacaa ggagaccttg aattttgagc cattgatttt
     8221 ttttctgata ccattctttg tccagccctg tttcgggacg tggataataa ttatgccaag
     8281 cttcaatctg tgagaaatca gcttctgctt ctttcaattc atcgatatct ttaggcgtga
     8341 tcgtgctagc attcaaactg attttcaatt gatgtgacca gtcggcaatc tgctgattgg
     8401 aaatatgata atccattctt aaacctgtta caccaattgc tttcaaaggc gttagatctt
     8461 ctaatgaaaa acccgctcgt tccaatgcct ctcctgaaat atcgaccatc aattctagtt
     8521 tattcttttt cgcttcgctt cctagttcga tcaatcgctt ttgatatgcc gaaacatcat
     8581 cttctggtat atgcatggaa gtgaaaatac cgacaaagcc attggatgcc atttggcgga
     8641 tatactgttt cttctcttct gacaaagaat cgttcataaa tattgaaaag ccaaacatat
     8701 tatccctctt tttctgtatc cgtcttcatt tctatgacta gagtatatat ctaaagaaac
     8761 aaaatttcaa tacttttatt aaaataatta aatttcattt ctaaaggttg agtttatatt
     8821 atcaaaagaa ttgtcataac ctaaaaaagg cactgctaag caaaagcagt gccttgaaat
     8881 attttattta aataacacaa aaacataatt actcataaga aaccaattct gtattatcta
     8941 ccatgaatgt aaagaacccc tcattaggca tcggatgaaa ataagcattg atcactctta
     9001 tcaaggcatt atggcaaact aacagataga cattttcttc gtcttcgatg cattctttca
     9061 ataaaggaac gatccgtgca tacacatcta agactgactc cccatttggg aatcgtacag
     9121 cgaacgctaa acgtgctttt tgaaagttct catctttgct tggcagtcca tcataatccc
     9181 caaaatccat ttctaccaat cgttcatcaa cggtcagcgg caagctgaga cgatcagcaa
     9241 ctgcttgtgc tgtatctctt gctcgttgaa gaggggaatg gatgattttc gtgattggaa
     9301 caggcaaatc tactaccttt tctgctagtt tctgtgcctg catctgtcct ttttcagtca
     9361 gcggcacatc tgcccgtccg caaactaatc cttgtgcgtt ccaagttgtt tctccgtgtc
     9421 tcgttacata aagcatgtga ttctctcctt cgttggcaaa gttcccctta accatacctt
     9481 gttgttcttg agattacaag ggttagattc tctcaagaga aataatctca gtagatactt
     9541 tatccaggaa ggtttgatca tgttccacta gtaacatggc aggtttcacg ttcaagatca
     9601 gttgttctaa ttgttcttga ttgaagacat ccaaataatt caatggttca tcccatgtat
     9661 atagttcagc tggctgtgat aaagatttag ccaattccac ttttttccgt tggcccatac
     9721 tcatctgctc gatcttgtta tgaaaaacat ctctttccat cccaagcttt cggaggttgt
     9781 tcaaaaatgc ttggtagtct acttggtttt tctctgcaaa ttccgccaac gttcctcgat
     9841 tgtcttcata attttggctt gcataactaa tgctcagatg ttttggctgg tatttttctc
     9901 ctataacctt gccgttgaat gcccccagaa gataatggat aatggatgac tttcctgcac
     9961 cgttaggacc tgaaatcgcc acccgctgat gaggctcgat tgtaaaatga attggctcga
    10021 ataacagatt ttcataccct aattgaagat cttctacgct taaaagtcgc ttatggtgag
    10081 acgcttggct attcatcgtc aaagaatcga tatattcgat atcttttaat agtttttctt
    10141 tctcgctgat ctgggtctcc atccgattca cgattgcttt ggatcgtttc atcgtccgtg
    10201 cagcatcagc acccactgct cctttattca ctcgtctaga ttcagtatcg atgaatccga
    10261 cttgtttctt tgttttatct ccttctcggg aacgagacca ttcggctttt tcagctgctg
    10321 ttttctttag cctactgact tcttttttca atttttcatt ttgagccatt tcaaattcat
    10381 cacgaagttt tttctgttct tcatagatag agaaattccc ttgataaagt tccagttgac
    10441 ttttttcgat tgctaaaaca tggtccacta cttcatcgat aaatccccgg tcatggctga
    10501 ccacgatgaa gccttgtttt ttctttttca aataagccgc tacttgtttt ctaccagaga
    10561 tatccaaatg attcgttggt tcatcgatta acgggaaatg agtgtcatcc acaaataaaa
    10621 gtgccagtag gacttttgtc ttctctcccc ccgatagtgt gctgaattct ctccataaga
    10681 tttcaggatc tgtttgcatc aattggagct ctctttcgat ttcccatatc tcaaaatccg
    10741 taatatcatt taacacgtaa taggttaaac gttctttatc ttttgtctgt tgcgggaaat
    10801 aggcaaattc ttgctgatgg attacttgcc cttgataagg tagtttgttt tgtagaatat
    10861 tcagtaaagt tgtctttcct cgaccgtttc gtccgataag tcctagtttc cattgtgtgt
    10921 caaaatttag atttgcttgt tcaaataata atgtgccttg gctgtcgtag ccgaatgtca
    10981 gattttttat ttcgatttta gacattttcc atcacctcgt aaaaaaagac cagtcctatt
    11041 ttcagaactg gctaaagagg atgcaagggt tggatacaca caaaaagacc catacatcga
    11101 aaaaaccaag ccaatcccga aaatctgcac actgcttcct tgtctccaca aggtacagtc
    11161 tgaatgcagc tttcatcaat cgacttaatt tttcaatgca cggatccctc actttgtcct
    11221 atatttgaaa ttacgatagc ataaagaaaa tgattttgtc aactacttcg ggaggatggt
    11281 gaaaaatctg ctaagtgtct gtcctaagat ttttgttttt tctaatgttt gcctgtgttc
    11341 cattgcttct tctaaaccta ttggacccaa atgaatactt aagacaattg ggagaaaatc
    11401 agaaagtcct tctagctctt ggtctctact tccgcaaata gcgattattg gttttcgata
    11461 tttttttgca agcactgcta caccgtatgg aacttttccg tggatcgttt gctgatcgat
    11521 ccgcccttct cctgtaatga tgacatctgc ttgttcgatc tttttttcca atccagtgat
    11581 ttggctgact aaatcgaaac cagaaaccat cttcgctccc aacaaaaaaa gggctccacc
    11641 aataccgcct gctgctccac ttcctggaat atcatctaaa tccacctccc agaaatcttt
    11701 tatttgctga gcaatctttc ctgcttgttt atctaagaaa gctagcgtcg tatctattcc
    11761 tcctttttgt cttccaaatt gccaagcagc tccttgttga cctgtatatg gattggtaac
    11821 atctgcagcg ataatcaatt ttactttaga aacaagaacg tttcttcgat caaaatgaag
    11881 ttttttagta gataataaag gatttccttc tgtgtaccct tctaaagaag cacccaagga
    11941 ttggagtaat cctagaccac catcgctaca cccgcttcct cctaaagtga caataatttg
    12001 ttctacttct tgaagcagtg catctttaat aagttctcct aaggcataac tggaagcaag
    12061 tcggatagtt tgatccgttg gtttatcaag tagatctaac cccaatgctt gtgcagattc
    12121 gatgactgcg gtgttttttc cttgccaagt tgtcaataaa tagctgattt ttcgctgacg
    12181 gaataagaga tccatcccat caatggtacg ccatgttccc ctgagcgcgt aatagattgc
    12241 ttctgcagta ttttctccgc catctgcaat cggcacattg atttttcttc ctttacgatc
    12301 ttcccagcca tttagaactg cctgatttag ttcaaatgaa gaagcacttc ctttaaatga
    12361 atcaattgcc gccaaaatat ccatctgtat ccctcacttt cttcttaagt aagacacttt
    12421 ttttcatttt tttcaaacaa aacaatgcta ttttcttcca tttttctata tactaaaagg
    12481 gaactcattg aataaaggag agaaaaaaca tgaaaatcgc aatagctggg gccggagcaa
    12541 tgggcagccg aatcggattg atgctacatc aaagcggaaa tgaggtcctc ttgatcgatc
    12601 gttggcctgc tcacattgaa gccattcgta caaatgggtt gattgctgac tttaacggta
    12661 aagaagtggt cgcaaaactg ccaatctatt caccagaaga aatcatcgaa agcaacgaac
    12721 acgtggactt gatcgtagca ttaacaaaag ccaatcaatt agatgatatg ttttgttcga
    12781 tccaatccat tattactgac aatacttatg ttttgtgttt actaaatgga ttaggtcatg
    12841 aagatgtact ggaaaaatat gtgccaaaga aaaatatctt atttgggatc acgatgtgga
    12901 cagctggtct agctggtccc ggaaaagtca cattgttagg tgatggagaa atcgaactag
    12961 agaacttgga accagaaggc gaagcattta caaaaaaagt tgtagaagtc tttcaagaag
    13021 caaatttaaa tccgatctat agccataacg ttcgttattc catttggcga aaagcttgtg
    13081 taaatggaac attgaatggt ttatgcacga ttttggattg taatattgct gaactaggcg
    13141 cgcaaaaagc ggcagagagc atggttcgaa caatcgtatc tgaatttgct tctatcgcag
    13201 caaaagaagg aatcatacta gatcaagaag aagtgtacca acatattgct tctacttatg
    13261 atcctgacaa catcgggttg cattacccat ctatgtacca agacctgatc aaaaaccatc
    13321 gtttgactga aatcgactac atcaatggtg ccatttggcg aaaaggacaa aaatatgata
    13381 ttgctacacc atattgtgcc tttttgaccc aattagtcca tgcaaaagaa ggaatccttg
    13441 gcgcagaata aaaggctctc tgatgagaat cagctgaatc agcagctggt tctctttttt
    13501 taagcccaat aaaagatcaa aaagaaaaca caactctcat gatgccataa gaattgtgct
    13561 tgtttgtttc agtacataat ctcttcaaaa tgtaggtttt ttatttgttt tcaacagagg
    13621 agaggtcata ttcgtattgc tcataaatag tggaatcgtt agtgataaat cgttttgccc
    13681 aattttctgc atcataaaat tccaatagct ctgataggat tggcttaaat ggtgtatgca
    13741 gaaattccgg taattcatct aaatcacgtt ctcgaagcaa aaaaatattt tcaggacgat
    13801 aaaaatgata atgagcaatc gcttgattat ccgtcactag tttcttttga aaatacatgg
    13861 attcaaatgt ccgcaaagtc actccttgct gaccctcccg caacagttcc agaatcccgc
    13921 gcgattcccc tgtttttttc aggtactctt cataagtaag atatccaggt tgttcattcc
    13981 ctcgatgtgg aagaatgaaa tagttggtcg taacatctct ttttataaat tcctgcttat
    14041 agctttctgc tcgttctttt ctcccattat tcgttgcacc aaaaaataga tccgtctttg
    14101 gttcgctaga agaaaaattc atatgtttcg aataaaatga actattatgt ttcagcccaa
    14161 gttcttttgc ttccaaaaaa tcaaaatgat aaatctcatc gattgccggt tcatcttgtg
    14221 cctgcttcag catcgcctcg tgttctgctt tgaagtgatt ccaaaaaaat aacacaattt
    14281 ttgcattggt aaatgggcgc acgcgtcgag ccatctccat atcaaatcca taatctaaaa
    14341 aaacgatcag ctgataattt gccaactgtt gtctagctga tttaatgaat tccgactgta
    14401 gtaaatcttt tccggaccaa acatctaaag agcggatgga aggcaaaaaa taaagtgatt
    14461 tattttttgc aactagcagt acgtcatttt tgttcatctt ttccgcctca ctttctctgt
    14521 cactttttcc tatgatagcc tgtttaaata gtaaaaagcc agtaaaacgt ttcctatatt
    14581 tgtatgaaaa tttaacattt gtatgaaaat atgatgaaat aaatcattct tctattaaaa
    14641 ttcgcctaaa agttttctga atagttgtaa ttgtctgaaa atttgttatc attagtcatt
    14701 gttaaatatt gctatttatt cttaatataa attttgattg tgaatttaaa agaaagaggt
    14761 catttatgga agataaaaaa ttgaaaaaac aacttagctc cagacatatc accatgttag
    14821 ctttaggtgg agcaatcggt gcaggtctat ttaaaggaag tggcgaagcg attgggatcg
    14881 ccggcccttc tgtattgatt gcttttttga ttggtgggct gattttatat atcgtgatga
    14941 aaggtctggg aaaaatcgtt ttaagcggtg gcgacacgca tcacggatta tctggtttga
    15001 tacgccctta tctaggtgct cattctgctg attttactga ttgggtctac tggtctatgt
    15061 ggattatcaa tatcatcgct gaagctgttg cagctgcttc ctttttgcag ttgtggttcc
    15121 cgcacattcc agcctgggta tttgttttta tgttggctgt tttaactacg atcatcaatc
    15181 tttattcggt acggttattc gcagaaacag aatattggct ggcttttgct aaaatcagtg
    15241 tcattatctt actgatcatt tttgctactt atcttgtcgg tcagcaaatg ttgggcacag
    15301 gcgttttccc tacccttcag caattgactg accatggtgg gtttactcct catggtatga
    15361 agggaatcat cagttctttg cttgtagtta tctattctta cggcggttct gaactgattg
    15421 ctatcacggt aagcgaagca gatgatccta aacgtgcgat tcctaaagca atcaaaggtg
    15481 tcatgggaag aatcatttcc ttttacatca ttcctttgtt tttgcttttg attctttttc
    15541 cttggaatac tttagcaggc acgaatgtca gtccttttgt gatggtattt gaaaaaatga
    15601 atatcccatt tgcagcggat atcgtcaatt ttgtgattgt ccttgctttg ttctcatcta
    15661 tcaattcggg tgtgtatgca tcttcaagaa ttttgttctt ccgtttgaag gatcgaaaag
    15721 gttcctctag aaaactagct gttttaaaca agcatcaagt accgcaacgt gctgttttat
    15781 tttgtaccag tgttctttat cttggtgtag ctctttctta ttttgtcggt gacaaattat
    15841 ttggctatct ggcaggttcc ctttcgtata cagtattatt gatctggatc attatcagtg
    15901 cggcatcctt tgtactggca ctaagaaaag gaacattatg ggataaagga atcagctttt
    15961 ttgcattagc tgtcttagga ttgattttcc ttggtatcct gttcacgaac tccattgggg
    16021 taactgtatt gactggcctg ctttacctgt tcatttattt cagttatcaa aaaaagaatg
    16081 atgcgttcgt attgacgaat gaatgaaatc gatagaaaat tttattttat ttatgtggaa
    16141 tagatggagc tattgaagaa aggagttgtg acaaaagtct tgttgcaact cctttttccg
    16201 aataaacagt ctcatgacct cttggtaaac ggtgttcaaa aaccctctgt gcaaaataat
    16261 tgattgttct ccatgctcat gacatgatag aagaaaggaa tgaaaaaaag gagtttgttt
    16321 taaatgaaaa agtattggaa gtttttgttg atgatcggtg tttcaacggt agttatgttt
    16381 ggtatgatgt acttgaatgt ctatgcgctc gatcatatct tcttcagtcg tacacgagta
    16441 ttcatggcgt tgatgatggg tgcagtcatg gcagtcatca tgctgttgtt catgtggaaa
    16501 atgtatgaaa acaaaaagat aaatgttatg atcttagccg ctagtcttct tgtttttgcc
    16561 ggttccttgt ttatggtacg aagccaaact actgtcaacg acacagcttg gatgaaggcc
    16621 atgattcctc atcattctat cgctatcctg gcaagtaaac gagctgattt aaaagatcct
    16681 gaagtaaaaa aattagcgga agaaatcatt caggcacaag aagaagagat cgacaaaatg
    16741 aaaaaaatga tcaaagaaat ggaatgacca ttatccgtcc aaataaaaaa agaggatgtt
    16801 ccagaagtta tcagcttcaa gaaataagaa gaagctactt ctggaacgcc gtgaataagg
    16861 ggttgtgaaa aagctgccct gcatcaagta ataagaacag ccaaacaaaa atagcttctc
    16921 ctatttttcg tttggttagg cttattaccg cagatgcaag cttttgaaca ccgtttatta
    16981 agaggttgta aaatcagcgt tctgacctga gtattagaga gacaaatgga aaaatagttc
    17041 ctcatatttt gccatttttt gagctaatac cgagggtcag ctgattgaac accgtttatc
    17101 gagaggttgt gaaaaagtcg cttagtttcg cgtattaaga aaaattttgt gaaatggctt
    17161 tccacatttt tgtataaatt tggcttaata ccgagaaact ggcttttgaa taccgtttat
    17221 tcgtaaaagg gattgtgaca aaagtcttgt cacaatccct ttttatgttt ttattgttct
    17281 aattgcgctg tcttgcttac tgcttcttgc ggtttcacaa atttttttga gattccccaa
    17341 cctgtgaagg cactgaccat cgaaaacaca ggagaaagca aactgaaaaa tacgaatggc
    17401 aagtaagtga gtgtaggaat accaaatgta cttgctacga aacttccggc aattccccaa
    17461 ggcactaagt agttgattac tgttccacca tcttctagcg ttctacctaa tacagttgga
    17521 tcaaatcctg ctttttcata agtttcttta aatgcattac caggcagaat aacagatagg
    17581 aattgttcac caataaaaat atttactccg atacttgtca tcaatgttgt aaaaatcaaa
    17641 tttggtgtgt tcgtcatctt tcttgatact cgttccatca cggttgagat caagccaaat
    17701 tcgatcaaca atccacctaa tgacaaagtt agaataatca atgacacagt aggcatcata
    17761 ctttcgattc ctccgcggct aagcagtgaa tcgatttgtt ggtttccagt atttgaaaca
    17821 aaccctgatt cgatgacccc agccaacttc gttaaggaag tttgtgggtt ttgaataaaa
    17881 atcattccta ctgctacagc gatattcaac aaaatcgtta aaattgccgg cacctttttc
    17941 catgcacaca gtaacattaa aaataaagga atcagtgacc agattgaaat agtaaagttt
    18001 gcttctagaa catcagtcac ttcacgaata gatgaaagac ttgcggcttt acttgtgtgt
    18061 cctaaaatca tgaataaaaa gaaagaaaca acaaatgccg ggacagttga ccacatcata
    18121 tgcttgatgt gtttgaacaa atcggcatca acaatagctg ctgctagatt tgttgattcg
    18181 gataatggag acatcttatc tccaaaaacg gcacctgaaa caatagctcc aacaaccagc
    18241 gctggattta agcctagtgt tgtcccaata ccgaaaaatg caatcccaat cgttgacata
    18301 acagtaaagg cactccctac agcgctacct acgattgcac acacaacaaa tacagaaggg
    18361 acaaaccact ggacactgat caatttgaac ccaatgacca ttagagttgg aatgatccct
    18421 gcttgtatcc atacagcaat caatgaaccg actaaaataa aaataaagat tgggatgatt
    18481 cctggtttga tgccattgat gattccttcg tgcagccaat ctgtcgggta tctttttatc
    18541 actgcataca acatgaccat tccgatcgct gctaagatcg tgatattagg tgagaccttt
    18601 aatccgacaa cgccaatcgc aatgcctaaa acaataacca ttaatacaag tgttgcttct
    18661 aaaaaacttg gtgttccttt ttttcccatg ataatctctc cttttttagt tgttcttatg
    18721 ttctaaaaaa gttaagatat cggattactc cgccgcaatt catccgcgat tcctccttta
    18781 taaaaataaa aagacctgtt ttaaactaag gacgaatgac tccgcggtac caccttaatt
    18841 aaaaacagat ctgtttttca ctttcaaact tttattcaaa tgtatggact ttccatgtaa
    18901 ttcgtcaatt aggcttgatc ggttcacagc gaccaccgac tttctgaaca aaacacctaa
    18961 ccgctactgc gtgaaaagtt gacttgtacg aagtttacct tccataagat catttgtcaa
    19021 tgaaaaagaa aatatttttt catttttctg aaaattattt ttataacttt cacacacaag
    19081 tttatttttt actagaaagt agcgccctct tgtttttttc ttgctactat ttcttgatgt
    19141 tcttgcttga ttctttctaa tagctttggc tctttcaaca gatcaagagc tgtattggcg
    19201 agtagttctg ctgcaatcgc aatagaagca aggcctttgt cacttttagc agcagctttg
    19261 aattcttctg tgtgtccggc aatatatgtc tcagagattg ctaccgtagg ctggattgtt
    19321 ggtatgactt ggctgacatt tcctacatca gaagaaccga gacttgttct ttgttccgtt
    19381 tctatttctt catctggaac acctgcttct tttgcatgct ggaagaataa gtcatcaaaa
    19441 gaaggagtaa cgatgacatc atcaactgca ttttggaaca atccaaactc aaaagtcgtg
    19501 cccgtactta tcgcagcacc tcgtacgata ttttccactt tttcatatac tttatctaat
    19561 gtttgccgat ttcctgcacg taaatagaaa cgtccagcag cgtaatctgg aatgacgttg
    19621 gcagctactc ctccgtctgt aatgatccca tggatattga catcttttgg caattgcaag
    19681 cgtagagcgt tgattccatt aaacacttgg atcaatgctt ccaaagcatt tactcctttt
    19741 tcaggtgctg ctgctgcatg ggaagcgaca ccgtaaaatt tgatgtcgac tggatcattc
    19801 gctaaagatt ctgtcgtttt cccataacga aaagctggat ggacgcataa cgctacatcg
    19861 acatcctcaa aaaaaccttc tcgaacaaag ctgcctttag ctgatccgtt ctcgccccct
    19921 tcttcccctg gggtgccata tacacggatt tctccaccag tttcatcaat gaattgcttc
    19981 actatgctgg cggctaatac ggagtaagta ccaaacaaat tatgcccgca tgcatgacca
    20041 atacctggta aagcatcgaa ttctgccaaa aaggcaattg tgggaccggg tttattggaa
    20101 atataacgtg catcaaaggc tgttcggtgt ccagctactt ctttttctac ccaaaacctt
    20161 tctttttcta attgtttaat caacgctgct gaggaataaa actcatagtt acttacttca
    20221 ggatgttcgt ggatatctag agcaagtgct tgatattctg gtaaacgata ttggattaga
    20281 tccttcaatt ggtttgttga gattgttgtt gtcataaaaa attcctccta tgctagtcat
    20341 ttttcttatt ttctcccgaa tgtttcccat gcaggcacgc ttgatccttt tgatgttttc
    20401 tcgattactt tgactgtgtc ttctgtttga taagcgtcta ctattttttg atacgtttta
    20461 ttgttttcat cttcttttct tgcaacgatg atattcacat aaggacgtgc atggtcatcc
    20521 actggttcta agaagatggc atcagaagca ggattaaatc cagcatctac agctacaccg
    20581 ctattgatca ctgaaatgtc tacatctgac aaagcacgtg ctgtttggga agcatctaat
    20641 tcagaaatgt ctagattttt tctattctcg gtaatatcag aaacagtggg cgcttgtcct
    20701 tttgccttat ccactttgat caatccagca gtttgcaata acagaagagc tctgccgcca
    20761 tttgtaacat cattagggat tgctactttt gctccgtctt tgatatcagc tacatccttc
    20821 aaagtgttag aataaattcc cagcggtgca ttgacagtat ttccgatcga tactaaatca
    20881 gttccgtgtt ctttattgta attgtctaaa aagaattgat gttggaaaga attcaaatca
    20941 atcgatccat ctgctaaagc cacatttggt tgattgtaat ctgtaaattt cacgtattcc
    21001 aaattaatgt tctccttttt caaacggtct ttcacgttgt cccatacatc tgtatcttct
    21061 ccaataatcc ctagttttac gatagtttct tttttgtcag ttgtatctgc ttttccttgg
    21121 ctacaccctg ccaaccaaat aattgctaca aatgtaatga ctacaaaacg aataattttt
    21181 ttcatttttt ctcctccact ttcaatgtgt aattttttta atcagttgat tactgataaa
    21241 ttgacttaaa aaaactaaga ttagaatcaa taatgtagcc acaatcgtta catctgtttg
    21301 gaatcgatta taccctctcg atatagcaag attccctaat ccgcctgcac ctactgctcc
    21361 agccatggta gtcagtccga tcacgttgat gatagtcaac gccgagaccc gaatgatacc
    21421 aggcaaacct tctaacaaat acacacgtaa aatgataccg attggactgg ttcccatcgc
    21481 ttctgccgct tcgatcaccc caggatcaac ttctagcagt gcgttctcga tttgacgagc
    21541 aaaaaacgga acaactccaa aaatcaatgg cactaaagct gctttcgcac caatcgtcgt
    21601 tccaactaag aaacgagtag tcaatgctaa aagagaaagc aagataatga aagggatgga
    21661 acgaatgata ttgatcagct tatcaagtat ttcaaatagc agacggttag ctagtaatcc
    21721 attagggcgt gtcactacta acgtcacacc taagatgatt ccaatcatcc cgccaatgaa
    21781 tgctgtccaa aaagacatat atagtgtttg cttcgtcgct tcgacaaatt catccggaat
    21841 caaataaaca ttgggcaaat atgttttaaa gaaatcagtc attttatctt cctccttcga
    21901 tcactttgat acgactatct aaatcgtttt tttgtaaaat atttattttt acaccagatt
    21961 tgatcaaata atccaatgct tttttccgct gtttggtttc tccagctaaa gtaacgatca
    22021 aactccctaa cggcgtttct tgtaataact ctacattgcc atataaaata ttcgctgtta
    22081 cttgatattt gctgtacaga tgagcgatta gaggttcgtt cgtctgattt ccaatataac
    22141 ttaattccac cagccattta ttggcgatct gatcggcaaa agcagaatgg gcaatgatcg
    22201 tttctaatgc ttggtcaaga tgagtagctg ttcgaataaa atctttggtc aattcttctt
    22261 caggattgct gaagatctgg atgctgctac ctttttcaac gattcttcca tcttccataa
    22321 ccgctacctt gttgcagatt tctttgacaa cttgcatctc atgagtgatc aggacgattg
    22381 ttaaacctaa ctggcgattc aattttttta acaatgcaag gatctgaagg gtcgttttcg
    22441 gatcaagtgc actggttgct tcatcacata ataatacctt aggttcattc gccaaagctc
    22501 gtgcgattgc tactcgttgt ttttgtcccc ccgacagttg tttaggataa gcagatgctt
    22561 tttcttccaa accgacaagt tccagtagtt cattcacttt ttgacgacgt tcctgtttcg
    22621 attttccaga atactttaaa gaaaaatcaa cgttgtcaaa aatagttcgg ctttccatta
    22681 gattgaaatg ctgaaagatc ataccgatgg cctttctttc ttttcgcaat tctttgggag
    22741 agagctgact cagattcgtc tgattgacaa tgacttctcc tgctgttggt ttctgcagca
    22801 aattcatcac acgaactaac gtacttttcc ctgcacctga gtaaccgacg attccataaa
    22861 catcgccttt ttcaatcgtt aaatctacag aatctactgc ctgtatctct ttttcttttt
    22921 gtttgaaaca tacagaaaca tctcgcaatt caatcattaa aattcctcct tatctaaaaa
    22981 aagcgtcctc aagaaaatga aaatgtcatt ttcttgagga cgcttttttg cgtgttacca
    23041 cctcatgttc aagctgtgtt cacacacaac tcctcttcaa gtacgatcat actctagcaa
    23101 gatatcggtc gcagccgtca tcgccttaca tctatgctcg ggatgaattg ctcgatggcc
    23161 attttccaat agtcgttcct tgcttcttct cagctaccga agctctctgt caaaggttct
    23221 tctatcgtac ttaccatttc aatgcaaata atattcaaaa aaaaagtcct tttactaaca
    23281 taaactatgt cagtaaaagg acgacgtatc gcggtaccac cttttttcgg aagtttcctt
    23341 ccctcaagcc caacatctca aaaagagaga ttggcgttcg ttttaacggt cgaacagttc
    23401 cgaatttgcc tacttatcga caaattattc tccaaggcca ttttcagctt ttcttacttg
    23461 actcttctca ccagccagag cctctctgca aagtttccaa acttactttc cttttcaacg
    23521 aaatcgatat tttgttgttg ggattaatct acatgaggaa aactgaaaag tcaagtacga
    23581 aattcaaatt attttttcta tatttatttt tctgtttttt tatttaacat ttcaataaca
    23641 gttcgaagat cttttccttc cgcaagaagt ttcaattttt ccttttcttc gacggtataa
    23701 tttcggacaa aactggaatc aggtatcaaa acaacgccta atcctgctcg tgaagcagct
    23761 gttgctccta ctaaagaatc ttccactgca aaggctgaac tagcttctaa tcctgttcgt
    23821 tccaaagctg tcagataagg aatcggtgaa ggcttgttct ccgacacttc gtctccgaat
    23881 gtaaacgtgt ccaagtaatc agctaattta aagtgatcca agatatctgt tgctcttttc
    23941 gtaacagtgg tcgtagcaat gcctagtttc atatgacgtt cttttaagtc tgccaatatt
    24001 tcttttgcat atggttttaa gtggatggct ccgcttgtca gctgttgata aaaatattct
    24061 tcacgcttcg ctcggacttc ctgtactttt tcagggctac ctgctgcttt cactaaaata
    24121 tcaaaacttt gtttccagtt ttttccgccc caagcgtcaa cgatttcttt tgggatatgg
    24181 atatcaaatt gctgaaatcc ccaaagccag ccatcacgat acgttttttc tgtgtcaact
    24241 aacaaaccat ctaaatcaaa tattgcacct ttcatctata cattaatcct ttccaaaaca
    24301 atacacatag tttagcacga ctggtccaaa aagaacagaa gcaaagaacc agccttctcg
    24361 tttataagag ttcttggaat cgacggatat agattttctt cacagctgtc acaagcgcca
    24421 gataagcgat gactgtagca aataaccata accagtaatt gccaggaagt ggcatcaagc
    24481 ctaattcagc accaaaactt gtaaaaggaa ggacagaacc gacaataatt cctaatgtag
    24541 tgataacaaa catggcaaat gttgcgttgc tttggataaa tggtactttc ggtgtacgca
    24601 gtgcatggag cactaaggtt tgtgtccata gtgattcgac aaaccatcct gcatggaata
    24661 atgcgatgaa aatcactcgc tgtgaactat ccaatgtatg gaaacttccg cccactacag
    24721 caggacaaat aacaaaatac atcaacaagt aagtagtaat atcaaatatc gaacttgttg
    24781 gaccaaagta aaccatgaat tccccaatac tagatgcatt ccattttttt ggctcgtcca
    24841 aatattcttt atccatccga tcccatggca aggaaatgca ggaaacatca tagatcagat
    24901 tcaaaaacaa aagttgtaaa ggcagcattg gtaaaaatgg caggaacgta cttgcaatca
    24961 gcacggaaaa catattgcca aaattagaac ttgctgtagc cttgatatat ttcatgatat
    25021 tcccaaaagt ttctcgtcca gaaagaaggc ctctttccaa aatcgtcaaa tctttttcta
    25081 aaaggatcac gtctgcagat tctttcgcaa tatccacagc tgtatctaca gaaataccga
    25141 tatcagccac tttcattgca ggagcgtcat tgataccatc tcctaaaaat ccgacagtat
    25201 gaccattttt acgtaaagct gtcgtcaaac gtgctttttg ctgaggattc aattttacga
    25261 atacttcatg gtcttcagct acttgtgcta attcatgatc actcatttcc gagattttct
    25321 caccggtaac caattcattg atctctagac ctacttgacg gcacactgaa cgtgtaacta
    25381 attcgttgtc cccagtcaga acttttacag ctacaccatg ttctttcaga gctttcaacg
    25441 cttgtttcgt tgtttctttt ggtggatcaa ggaaagcaag ataaccaatc aaaaccatgt
    25501 ctgactcatc tttgactgaa aattctccaa cgacgcttgg attggtcttt tgtgcgacac
    25561 caatcactcg taagccatct tcattaagtg actgtacttt tgcaagtacg gcttcttttc
    25621 gttcttttgt taatggtgta acagtaccgc caagatcaat aaatttagaa atgttcagca
    25681 tttcttcaat cgcgcctttt gtgatcattt gtgttttacc agctggatca gcaaccacaa
    25741 cactcatacg acgacgttca aaatcaaaag gaatttcatc cactttttga taattgatct
    25801 tatctgtatc taaagtttct gttgcttcat caatgatagc cacatctaac aaatttttca
    25861 gccctgtttg atagtagcta tttagatacg catggcgcaa aacacgatca tcttcatttc
    25921 cttcacaatc catatgatat tcaagaatga ttttatcttg tgtcaatgtc cccgttttat
    25981 ccgtacataa cacatcgatt gcaccaaagt tctggatggc attcaaattt ttgatcactg
    26041 ttcctttttt ggccatcaca cttgctcctt tgaccaaatt cgtcgtaacg atcattggaa
    26101 gcatttcagg tgttagacca actgctacag atagtccaaa aagaaaagct tctagccaat
    26161 ccccttttgt aagcccattg atgacgataa ctgctggagc catcaatgac ataaatttga
    26221 tcagtagcat ggatgtttta tggatgccta attcaaagct tgattgaatt ggtttttcag
    26281 atagcgtttc tgctacatga ccaaatagtg tttcatttcc aacagaaaca atgattcctt
    26341 ccgcacttcc actgatgaca ttacttccca taaagacaag attttcgtaa cttgtttcgc
    26401 tttgctgatc gtcttcacat actttcgcat gcttttctac tggatagctt tcccctgtca
    26461 atgctgcttg ggaaatgaat aaatcctttg cggacaataa acgcatatcc gctggtatca
    26521 tatcccctgc tgaaagccga acaagatctc cacagacgat ttcttccata ggaatctctg
    26581 tgtactttcc attacgtttt acagctgccg ttactttcac aagtgatttc agtttttcag
    26641 ccgcttgatt ggaacgtacg gactgaatca acgtcattgt cccgctgacg ataaccatag
    26701 tgaagataat ggctacaccg aaaagatctt tatctccagg agctgctaat acatattccg
    26761 tgatgaatga aataattcct aatgcgatca acactaatgt aaatggcgtg atgtatgctt
    26821 tcaatatttc tacaatgaat ggtgtttttt tgccatatga aatcgcattt tcaccgtaat
    26881 tttctcttga acgttcgatt tgttcttctg tcaatccatt tgctgatgca tggaatagtt
    26941 caaataattc ttgtttcgtt tttccagcaa aagctgcata gattttttgt gtatctttct
    27001 ttttttccat ctcaatctct cctttgtgtc caaaagacag ctgttctcgc tgcttttggt
    27061 ctttagctat tcatcggatc gatagagaaa aaactgaaag gggctgagaa cagatagata
    27121 atagccctgg cctttcctct agatccttgt ttttcgaatg actgtcatcc atttgttctc
    27181 atctccttta ctaagtaatt ttaattcatg acttccaatc atttttcgaa tagttatttt
    27241 tccaattaga ccacctcctt tttatttttc accgatgggg atttgcggca acaaaaaaac
    27301 ctctcgccaa atactcagcg aaaggtttga aaaagcatat taaaaaaagc ttctcagatc
    27361 tcatcgttga gttttgacac tgtacaacgt aaaaagcaga tacctgcttt tagctacctt
    27421 atgaaaagcc ttatctcgac aattcctgtt ctacccattg gcgtctctcg acgtttttgg
    27481 gcagtagcct atgtttgtat aggagcctca cctaacaaat cttattcggt tttccttcgt
    27541 taatataaca actaaaaaag aggatgtcaa atcaattttc catgtttttc atactttttt
    27601 taaaatacac acgttatatt cctgtctcac gcggttttat ttgtctcatt ttccacctcc
    27661 cgctataata gaaatatagt aggaaacgag gtgctgttat ggaaagaaca cttgttatta
    27721 tcaagccaga cggcgtgaga cgccatttag tcggaagcat catccaacgt tttgaagcaa
    27781 agggactggc cattgctgaa atgaagtttg aaactatgac tcctgaatta gcaaaagaac
    27841 attatcagca cttgactgag cgcagttttt ttgatgaact gatcgattac atgacttccg
    27901 gtcctgtcgt ttatcttgtg ctggtcggcg aggaagtgat cgatatcgtc cgtaagatgg
    27961 ttggagcaac aaaagctgcc gatgctgttc caggtacgat ccgaggagac tacgcgctgc
    28021 cggggactga aaacatcatt cacgcttctg attcccgtga tgctgcggta aaagagatcg
    28081 cccgcttttt cccgccattc gatataaaca aaccgtctga aaaagcggcg aagtgataca
    28141 aattctaaac tgctggtcta gttgctgggt tcagcaatgg ccagcagttt tttctctaag
    28201 gacctccgtt gattctttcc taacatatat tcgagcactg tgaagaattc ttggtagtct
    28261 ttcgtctttt cttcccctaa agcagcagaa atcaatcttt gataataatc tgccatcaat
    28321 gtttcataga tcactgtact tctttcttta tgtgataagc tccgtttcat atcttcattc
    28381 acactacgtg cctttttcaa ccgaccattt tctatataaa acgaagtcag gttcattaaa
    28441 aaagtaaaga gatactgctc atgattgttc aactgtgcta atcgtcgcat tctttttttg
    28501 attttcaaat aattgttctc aatgaaccga tcactgaata tccaaggaaa atcagctaga
    28561 atagatagtt caaattgacc atagtctggt atctgcatca agtattcttc aatgattcgg
    28621 cattcataac gtaaaaattc aaattctgtc tcagatactt ctgttttttc gaccatgtga
    28681 agattcaaaa ccaaataata cagatcttct gttttcttaa aatgcctacg gttattgctg
    28741 atctctttat aaggatcaaa gctgtcttct tgtacccgat ctagcattcg tctttttttc
    28801 tttaacttta ttggttcatc cagctggttt cgcatccatt catattctgc aagtgtcatg
    28861 ttcattcgct ctaaaaatga tctcaatgtt acaaatgaca tttttttcac tgaatttgtc
    28921 tcaatcccga ctagagaagt cttaaaacag attccttccg ctaattggga ctgtgtcaac
    28981 ttcttgttga ctcgaagttt cttgatcaat tcaccatcat acataatagt tctccttttt
    29041 aatttaaatt tttccacatt caataaatag atacgttaaa aaggaaaaat acacgaaaaa
    29101 aataaaaaaa tgtttttttc ataaaaaata ccaaccttca agcattagaa tgttgtctaa
    29161 ctactgaagg ttggtttgat tgtttattga ttattttttt gctgttcaac gtctgttctt
    29221 gttactggat ataagttggt tgttgtttta ttaccatttt ttgaaaagac acttgtcgaa
    29281 tgttcgcctt tttgcttttc gattgcttca ttacgttccg cttcattttt atagtcatat
    29341 tttttcggat caatcgtctc aaatccgttt ggcacataaa accgcaagag attttcctga
    29401 ttcagcttat ctgaaagact taacgcagtc ttgacttttt cctgatcttt ggagatttcc
    29461 tcatcgattc ctgctgcttt cgtatcgatg atttctcctg tctgattatt gtaagctttt
    29521 cctcctagga cggtgtattt gggtgtcaca aagttcccat ttcgaaaagc aactacttga
    29581 tcatgctgag aagataataa gtctgtacca aaatgaagat aatctttatc atcgattccc
    29641 aatagatgta gtaatgtcgg caaaacatcg atttctccac cgtattcatg attgactgtc
    29701 cctttagtat aaccaggcat atggatcatc aatggcactc gttgcatctg tgcgttgtca
    29761 aattcatccc atgtatctgg atctttacca agagcgcttg ctaaatcttt attatctgta
    29821 ttcgagatcc caaaatgatc cccgtaaatc acaaagattg aattttggta aataccagat
    29881 tttttcaaat acgtaaaaaa ttgttccaat gattgatcca gataatgtgc cgttcgaaca
    29941 taattatcta ctgtgctatt gccagtattc aaagatggaa agtcgaaatt cttgtcatcc
    30001 gtataataag gcgtgtggtt ggttacggaa agaaatttcg tgtaaaacgg ctgttgtaga
    30061 tgctcaagat attgggcaga ttctctgaat aaatctttat ctaatatccc ataacctaac
    30121 gtttcgtcgg attcatcaaa ataagatcga tcaaagaaat tgtcataacc taagttttta
    30181 tatacatgat ctctattcca aaaactagct acattcccat ggaaaacagc gcttgtatag
    30241 ccttgctttt gtccaaggat cgctggtgcg gcttgaaaga cattgtctga cccaagttct
    30301 gtgaataatg agccttgagg taaaccaaat gtgccagttt ccaacatatt ttcagcatca
    30361 cttgtctttc cctgtcctac ttcatgaaag aaattgtcaa agctgatcgt tgcttgattc
    30421 tggaaaatac tatttaaaaa cggtgtgact tcttgtccgt ctaccttcat attaatcaaa
    30481 aactgctgga aactttctaa atggagaacg atcacatttt tccctttagc gatcccaaat
    30541 gtttcgggat ttggctctgc ataatgtttc tttgtataat ctaaaacttc atcaatccca
    30601 ttactgcttg catgcgcgcg aacactgcta gtcattcctg ttttgattcc gtcataaata
    30661 gtatacgcat cgattcctag atatttgaca atatatgaac ggtcaaatgt tcgctgcagc
    30721 aattgtggac ggttggcttc gctcagcgct agatttcctg aaaaaataag ccctgaaaga
    30781 caggtaacag cgatagccat cggtttgccg acagggttgc ttttgatagg tattttcttg
    30841 accttcaacc aaaccagtag cccaatgaac acagcgatat ccagccagta gatgacatca
    30901 tggggcttca tcaaagaaaa ggagcttcct gacaatcctt gcgacacttt cgaaaagcct
    30961 aatacagatt tgactgtaat gaaatctgtg aattcccgat aaaaaataat attcaagtac
    31021 aacaaaagcg tatttaaaat atcaatgatc aaaagaatag ctaacgctgg ctttggcttt
    31081 ttaatataca aagaaaggcc aaaaaacaat aaggtagtcg caattgggtt gatccacaag
    31141 ataaagtact ggatcgtacc ttccacacct aatgaaaaat ctgcatagta ggcaatgatc
    31201 gtcttcagcc agaaaaagaa aacagctgtc aaaacgattg ctaaacgatt ttggcacaat
    31261 tttttaagtt gtgtcacgta tatcacttct ctctattcac attttttctt attttgctcc
    31321 tgaaaaatga aaatttccta aaaaagttgt tatcttaaga tttttttatt ctttctaaag
    31381 attattccgt ccacttttca cagcataaaa ccgctgcgat gtaagcgatt aaactaatga
    31441 agagataaaa gtcttgtggt gaccgcttca ctccacggta ttcaaaagcc agtttctcgg
    31501 tattcgtaca aaaaaagagg ctgtgacaaa attcttgtcg cagctcctta tttcttgatg
    31561 caggacagct ttttcacaac ttcttcctta aacggtgttc tcctgccaac tcctcggcca
    31621 caagtcacaa tatttataaa acatgagaag ctgttttttg aaattgttcc ttgtggctca
    31681 gagttaaacg gcaggctcaa gacctcttga tacacggtgt tcaatcagct gaccctcggt
    31741 attagattat gtcaggacgc taattttaca acctcttggt aaacggtgtt caaaagcttg
    31801 catctgcggt aataagccca accaaacgaa aaatataagg agctattttt tgaaattgct
    31861 ccttgtggct cagagttaaa cggcaggctc aagacctctt gatacacggt gttcaatcag
    31921 ctgaccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt gccgtttgtt
    31981 tctctaatac tcaggtcaaa acgctgattt cacaacctct gtttttcatt gtccatagga
    32041 ttgatattgt ccttgtgatg actcattcgt aataattgag gcttgatttt cttcacttcc
    32101 cgttatcgtt tgtccactgc cagaatctga aggacttgtt cctgtcgact cgtttttttg
    32161 agaagagaca gttgttttct tgacttgttc atttgtcttt ttccattccg ctaaacgatc
    32221 ggcgaccact tttttgtcta ctttttcaga ataagcggtg atcacttttc catttttcaa
    32281 acgggctact ttgtcgccat catgagtcaa aagcaaaacg gtcaaatgct tgtctttaaa
    32341 ttgatattgt ctgagagaaa tcgcactaag tacgtcatcg atattaataa attgattttc
    32401 ttcttttcct tttggcaaat aaatgtccaa ttcgcctttt tcgtaattgg cagttatttt
    32461 atactccgcc caccaataag ttgagaattc ttttttgaaa ttatctactg attctttgct
    32521 tgctacgatt ttttctgttt ctttctgagc aagatctgct gtcatttttc ctgaaaatgc
    32581 tgcggataaa ataagcagta agaaaaaacc gaataaccaa aagagaagaa acggtctttt
    32641 tttcctcatg ctttcctcca ctcctattta attttgacta ttctattata tacaaaaaat
    32701 gaaatagaaa gacaaaaaag ttgaaaaaga tcgtaaataa ttgaacttct ttttcacaaa
    32761 atggtaagaa tggagaattt cacgcatttg ctctcaacta ttctttctta agcctcaaat
    32821 ccgtttttat ctgatacgtc tttgaaccaa tgacccgatt ttttgattgt tctttttcca
    32881 tcttgtgcta agtctactga aataaagcca tatcgattct tatatgcatt ggtccacgac
    32941 cagttatcca tacatgtcca catatgatag cctaaacaat tggcaccttc ttgaatggct
    33001 tgatggacat atttcagatg agattggaca aattcgatcc gataatcatc gtggatcatg
    33061 ccatcttctt tgataaatcg tgcttcgttt tctacaccca taccgttttc agaaataaag
    33121 cagtcgatat ttccataatg atctcgtaca ttgatcaaaa tatcatagat tccttgttca
    33181 tagatctccc atccgcgata aggattcatt ttacgaccag gcatttcata attgtcgaaa
    33241 taatcctcag gcattggacc gtttttagta tcgattggtg tttcttttgc ttttacccgg
    33301 cgtggctggt aataattgat acctaaaaga tctactgtat tttctttgat aaccgtcaaa
    33361 tcctctgcat ggatcacagg catgagatca agttctttta ctatctcaat caattcactt
    33421 ggaaactcgc cttttacagc tggatcaagg aatgaacgat tgaagaacgc atctgctatt
    33481 tgcgctgctt taacatcttc agggtttgat tcgtctcttg gatagcttgg tgtcagattc
    33541 aagatgatac caataacacc accttgctcc atttcatggt aagatttgat ggctaacgct
    33601 gaagccaacg cttcatgata gccaacttgg atcgcttttt tcatatctga ctcttctgga
    33661 taatgccatc gatataaata acctgcttcg actggaacga ttggttcatt atgagtaaac
    33721 cattttttta ctttcgaacc aaacaattca aaacaacttt tggcatattt ttcataggct
    33781 tcaaccgttt ctcggttcaa ccatccgcct ttttcttgca gatacatcgg catatcgaaa
    33841 tgatacagat tcacaaaggg ttcgataccg ttttcaatca actcatcaaa ataacgatga
    33901 tagaaacgaa cggcttcttc gttgattgtt ccagattcat caggtatcaa acggctccat
    33961 tggatagatg ttcgaaatga attatgccca gtctgtttca tcaactgtac atcctcttga
    34021 tacttcgtat aaacttgcga ggttttttca ggtcctactt gatcaaagaa cttttgcggt
    34081 tcttttttgt accaataatc aaaaatgttc tctccttttc catcgcctga aatccttcct
    34141 tctgtttgtg ggccacttgc tgcagaaccc caccaaaaat ttgctggaaa ttgatatttt
    34201 ttcatcaaag cacctctttt tcttttttag ttcgttttag ttgattccat gtatgatatt
    34261 ttccatcaca atcacttttt cattttcttc ctcttctttt ttgattcgtt ttaacagctg
    34321 ttctgctgct cgttccccga ttttatctgt tggtggcgtg atcgtgattt tcggaaggat
    34381 actcagatcg tttttatcta aataatcata gtgactgaac gcaatatcct tcccgattct
    34441 gacaccttct tgattgaaac attcgatggc accaatcgac atattgtaat tgctgacaaa
    34501 aatagcagtt gccccttttt ctaataatgc ttttgccccg taatagccgc tttttctaga
    34561 atagtctcct gtaaagacct ctaattggat cgatggatca ttttggaccg cttctttggc
    34621 tcctgctaaa cgttctcttg ctacgtagtc tgtttgagtg gcagcaataa atccgatttt
    34681 tttatgtcct tgttcaccat gatgagcgat cacttttgct acactttctc gatcattcac
    34741 taatattgta tcaatacctg ctggtccttc tggacaattc aatgcgataa ctggaatact
    34801 catttttgtg agcatttcaa atccagacca ctcttcttcg gataagaaga caatcagccc
    34861 atcgatcaca tgcttcatca ggtattcaat tttttgttca attttttctg gctgatcggc
    34921 aaacccgctt aataataagc tatacccatt ttcttcagct actctttcga tccctgagac
    34981 aacttccgac ccaaaacggc tggaaatccc gtttatcaaa agtccaatag agaagcttcg
    35041 attattcttc aaccctttag caatgatgtt ttctttatag tccaacgctg aaatcgcttc
    35101 tgctattttt ttcatgttct tttccttcag ttgctgacca ttcaagtaac gggaaactgt
    35161 tcccactgaa acaccagcca tcttagccac atcacggatc gatgccatac attctcactc
    35221 ctgccagtta catttgattt ctctttcttg ttgttcgatt atgatgaccg cattctttgc
    35281 atagtgttgc ttgtcatcaa tatccatata actaaagtct tcagtagagc aacttcctct
    35341 cactattaat tatacttaaa caagtatttg aaccggttca tttttaatat agcatttttt
    35401 taaaagattg caacactttt ttaaaacgct ttctgaaagt ggcggatttt ttgccttttt
    35461 aaaattttat tctaaaattt actctatttg tgggtgtttt gtgcgttttt ctcttccatc
    35521 cgtttgaagt tttacattcc aatggtataa tttctttatc ctatcaaatg ggggaagtat
    35581 tttgaagaaa ttaatcgtac tatttatttt gtctgcggga cttttgacat tgactggctg
    35641 tgatttgttt ggcggctctg accaatcctc ggacgaaaca accgattctt ctgtcttaaa
    35701 agcggaaaaa gcagatacca ccaacagtgt atatacgcta aaagtacgta aagatatgga
    35761 tttatccgcg ggacagttga catttacaga taaaaaaatg gaatggacac gcacgtatca
    35821 gacacaagga actgattctt ctgaatcatc tgaagaaaaa acagtgctga cagatatcaa
    35881 gatcactacc aaagatgacg aatatattat ttcaggaaaa gaaaataaaa aagaaacaac
    35941 agtcatattt acaaaaatcg gtaattaccg tttgaaggat gaggatggca acatctacag
    36001 tctatagtgc cgtctgttgg atcttctttt gtttctcccc ttcttttctg ctatagtaaa
    36061 gaagcaagat gattgtaaag gagacgaaat ggatgatcga tagtcctgat tattgtacat
    36121 gtaataacaa caaaaatatc catacagaat ttaatgacca aggaagcttg agcatatgcg
    36181 acaactgtca caaactagtc aaagattcat ttaaaacatt tgaatcttat cctgttcaaa
    36241 attatgtaaa aagtagaaga agataaacaa atccaagcaa aactcactat ggctgatttt
    36301 tatgaacttc aaactcagct tcttcctttg atcaagcttc atcagtaaaa caaacagtgg
    36361 atcatttcag tttacaaatc aaagagtaaa gatttattta tttcaaatat ttgaatataa
    36421 aataaccaaa cgatagaaag gcttattcta catcacgtag taatttttct ttcccgtcgt
    36481 ttggtttttt atttttagcc taattcttga ccttttgcat gctggttatc cgtaaaatat
    36541 tttccagcag gctttccttc caattcagct aatttttctt ggatctgacg gatcaccttt
    36601 tttgcatctt ctggattttc tacaaaatcg aatttatccc catccacttg tattttagga
    36661 catacatcaa aatcttcata ccattgatta tagcgggtgt tcaacgtttc ataataggta
    36721 tataacgtct catcgctttc tagctgttca tagtcacgcc cacgtttttt gatacgttct
    36781 agcatcgtgt ccaaagaaac tttgatatgg accatcaaat caggccgttt ttttggagca
    36841 acatcatcta actcgtttag catcgtatct aataagctat cgtactgctg tacttcgata
    36901 tccgttaccc gacctaaatc agcgtttaga tggaacaaca ggctatcttc atagatggat
    36961 cggtctagca cattgtcatc gttcactaaa gcatttttca ttgctaaaaa tcgcttgttc
    37021 aaaaagaaga tttgcaataa aaaagcgtac tgctcaggat ttgaataaaa gagcggcaat
    37081 acttcgttat cttcgacatt ttcataaaat ggtttagaat caatctcttt tgcaagcata
    37141 tccgtcaaac tgctcttacc tgcaccaatc gttcctgaaa tcaaaatcac tgacatacaa
    37201 cccttcctct tctataagta ctcgcctacg taagtttcaa caggcaatgt ttcaaaatca
    37261 tattcacgta cagcttggtc tgattttaaa tacgtatgga gactttgtgt aatcatcaag
    37321 ttcgtaggta ccgtctcttc ttgtacaatg atcatcggtt ttttcaacat agttgccaca
    37381 cctaattcgt aagccgttcc tggatcgatt gtttgatgct caaagtcaag gatagcaaca
    37441 acaaattctg catttgtcag ttcttccata tctttttcgt atacttcctg cgcccatcca
    37501 gcgctgaata gttcataatt gctttcttgg tgatgacgtg gagagtagaa acttgtgact
    37561 gttttgtttt cctccaacgc tttttcgata cgacttactc gatcgatttg ctcctctgaa
    37621 aaaaatggtc ctgctaaata tatttgactc attattttgc ctccttcaat aataactgat
    37681 ttttaataga tactgctcgt aattcattga ccagttccaa tggattgttg gaaaagatta
    37741 cttgatccaa ccatttttta tccataaaat cctcttttgc aaaagtttct aataattgtt
    37801 gcaagccgct gtaataaccg ccaatattca atattccgat tggcttgtcg ggcgtcagac
    37861 cgattgctat ccaagaaaga agttgtgaga attcctccaa tgtacctaga cctccaggga
    37921 aaacgaggac agcatctccc ttttcaaata ataatacctt tctttcatcg atcgtttctg
    37981 taggaataaa tgtagtaaca tcttttccag gcaattcagt ttcaaaaagc ccattcggat
    38041 agatacctgt gacttttccg ttattttcca gtactgtttt actaatcact cccataatcc
    38101 cggagtatga accaccgtaa accagttcga tttcttcttg tgccatgtat tttcctaacg
    38161 tttgcgcaat aaatttgtaa ctttctttat taccaaaacg ggaaccgcaa aaaacagtaa
    38221 gtttcataaa aaaacactcc ctaactcaaa agtctatctt cattctacac gtgttttttt
    38281 aaaataaaag actagatttt gtagtttttt agtggtatat aggatagacg ccactatata
    38341 tagtatttag aaaaaaatca tttttcagcc attttataga taaaacaaaa ataaataacg
    38401 tgttttcatc gatatgaaat gacttactta tagaagatac
//
LOCUS       DAYYCW010000012        38100 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000012 DAYYCW010000000
VERSION     DAYYCW010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.012"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(267..1577)
                     /locus_tag="ACHAYA_000633"
     CDS             complement(267..1577)
                     /locus_tag="ACHAYA_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM8604462.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1589..3007)
                     /locus_tag="ACHAYA_000634"
     CDS             complement(1589..3007)
                     /locus_tag="ACHAYA_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8604463.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3247..4815)
                     /locus_tag="ACHAYA_000635"
     CDS             complement(3247..4815)
                     /locus_tag="ACHAYA_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM8604464.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4819..5229)
                     /locus_tag="ACHAYA_000636"
     CDS             complement(4819..5229)
                     /locus_tag="ACHAYA_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8604465.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5226..6149)
                     /locus_tag="ACHAYA_000637"
     CDS             complement(5226..6149)
                     /locus_tag="ACHAYA_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM8604466.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6146..7024)
                     /locus_tag="ACHAYA_000638"
     CDS             complement(6146..7024)
                     /locus_tag="ACHAYA_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM8604467.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7011..8348)
                     /locus_tag="ACHAYA_000639"
     CDS             complement(7011..8348)
                     /locus_tag="ACHAYA_000639"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM8604468.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8351..9454)
                     /locus_tag="ACHAYA_000640"
     CDS             complement(8351..9454)
                     /locus_tag="ACHAYA_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8604469.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9528..11036)
                     /locus_tag="ACHAYA_000641"
     CDS             complement(9528..11036)
                     /locus_tag="ACHAYA_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8604470.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11002..11694)
                     /locus_tag="ACHAYA_000642"
     CDS             complement(11002..11694)
                     /locus_tag="ACHAYA_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8604471.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11716..12690)
                     /locus_tag="ACHAYA_000643"
     CDS             complement(11716..12690)
                     /locus_tag="ACHAYA_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8604472.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12990..13862
                     /locus_tag="ACHAYA_000644"
     CDS             12990..13862
                     /locus_tag="ACHAYA_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8604473.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14320..16518)
                     /locus_tag="ACHAYA_000645"
     CDS             complement(14320..16518)
                     /locus_tag="ACHAYA_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8604474.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16750..17742
                     /locus_tag="ACHAYA_000646"
     CDS             16750..17742
                     /locus_tag="ACHAYA_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8604475.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17802..19223
                     /locus_tag="ACHAYA_000647"
     CDS             17802..19223
                     /locus_tag="ACHAYA_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM8604476.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19216..20715
                     /locus_tag="ACHAYA_000648"
     CDS             19216..20715
                     /locus_tag="ACHAYA_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM8604477.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20754..22148
                     /gene="uxaC"
                     /locus_tag="ACHAYA_000649"
     CDS             20754..22148
                     /gene="uxaC"
                     /locus_tag="ACHAYA_000649"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8604478.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22307..22990
                     /locus_tag="ACHAYA_000650"
     CDS             22307..22990
                     /locus_tag="ACHAYA_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM8604479.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22992..23828
                     /locus_tag="ACHAYA_000651"
     CDS             22992..23828
                     /locus_tag="ACHAYA_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8604480.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23839..25593
                     /locus_tag="ACHAYA_000652"
     CDS             23839..25593
                     /locus_tag="ACHAYA_000652"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8604481.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25869..26210)
                     /locus_tag="ACHAYA_000653"
     CDS             complement(25869..26210)
                     /locus_tag="ACHAYA_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8604482.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26278..28449)
                     /locus_tag="ACHAYA_000654"
     CDS             complement(26278..28449)
                     /locus_tag="ACHAYA_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8604483.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28660..29517
                     /locus_tag="ACHAYA_000655"
     CDS             28660..29517
                     /locus_tag="ACHAYA_000655"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8604484.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29588..30445)
                     /locus_tag="ACHAYA_000656"
     CDS             complement(29588..30445)
                     /locus_tag="ACHAYA_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8604485.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30430..33132)
                     /locus_tag="ACHAYA_000657"
     CDS             complement(30430..33132)
                     /locus_tag="ACHAYA_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8604486.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33145..34434)
                     /locus_tag="ACHAYA_000658"
     CDS             complement(33145..34434)
                     /locus_tag="ACHAYA_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM8604487.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34584..35630
                     /locus_tag="ACHAYA_000659"
     CDS             34584..35630
                     /locus_tag="ACHAYA_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8604488.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35632..37785
                     /locus_tag="ACHAYA_000660"
     CDS             35632..37785
                     /locus_tag="ACHAYA_000660"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM8604489.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11255 a         7833 c         6779 g        12233 t
ORIGIN      
        1 tcgaaatctt tcagcaactc atttatcata acttatcgtt tgttatttgt caagaacttt
       61 tttgaagttt ttttcgatag aatcaagctt cgcaagtggt tctttttaac gacttagtta
      121 tcttatcacg tctaaaaaca tgtgtcaact cttttttgga attttcataa aatgtttcaa
      181 tgaagcgcga caacgttgtt taatatatca ttaaaaaaaa taaaggtcaa gctaaaatat
      241 tatttaagct tgaccttatt ataatttcaa cgattgtcaa aagtattgtt tgcagctgtg
      301 ttgccagttt ctatagataa atcacagctt ttgaaacata agttttgtac tgcttcgatt
      361 cgcccgaatt cttttgcagt tatttggcaa tgagaaaagc agacatcttc tataggcttt
      421 tcaggaaatg ctactaaatc aaaagctcgg gaaggaagtg agtaacctgg agaaaggcat
      481 gcctgtacgt ttttgatgtg tatgtctttg actttcgtca tttgctttga ttcaggtatt
      541 tcttcagcca ctgcttgcca actttctggc atttgagcaa ggtcattgct attttttcta
      601 ttgtattcat tatgccagtc cattatccaa gaaaaaggaa attgtacatc aatcatattc
      661 agattttcta ccctgatatt ttcgatcact ccccctcttt cttttgaaga tttcattcga
      721 ataccacagt ctgactgaaa aaaatcgata tcgtgaatat atacgtcact aactccagca
      781 ctaacttcgc taccaatagt tacaccgtat cctgaacgga ttttacatct agcaacctcg
      841 attcttgatg attttcgatt gactcttgca ccattgccat ctctgccaga cttaatagca
      901 atacaatcat caccacaggc caaatcacat tcatagacac gcacatttgt tgaagaatca
      961 atatcaatcc catctgtact tggtccatca ttgttgcgga tgattacttt ttctactaat
     1021 acatcgttag aataagtgat ttgcaagttc caaaaacctg atttctgaaa aatcaaatca
     1081 cgaacaacaa catgttctgc atgatacagt aaacaagcgc gtggacgttt aatagcataa
     1141 tccgcaatcc aacgaagtcc cttttgatcg tattcagctc ttgtcccgct tttttgatct
     1201 tttccccaat acagctccca ccaatggtct ccttgaccgt cgattattcc ttttccttct
     1261 ataagaatat ctttcgccga gagaacattc aaaatcgctg ctggccattt catttctacg
     1321 ccggctactc gtgtatcgat ttctggaaat gcttcgatat ctgttctccc cttgaggact
     1381 gcaccttctt caaagataag atgactctta tcttttaaaa aaagggctcc gactaaataa
     1441 atgccttttg gaataaatac aggtaagtgt tctttcgcac cttgatcgat tgctttttgc
     1501 agtattattg tatcatcttg ttgagtgtcg tttgttttaa agttcaaagc attgataatc
     1561 cctttttttg tccgcaatac gtttctccct atctttcatt gatttttttc aattcttcac
     1621 caataaatac agtttttgaa cgattcagtg ggtaacctac tagcaaaatg attgcggata
     1681 caaataagca gaatgctggt aacaagtttg ccatcgtata aatcctttct tgtactaaaa
     1741 ctgattggat ggctccacct gttgtagacg attggtaacc aatgagtgcc agcataaatc
     1801 cgcctaccgc tcctgcacat gcttgtccta cttttctcgc aaaagaattt actccatata
     1861 ctgttccatc ttctcttaag cctgtcttat attgatgata atcaatcacg tcagtaatga
     1921 atgcccagac catcaaattg aataaaccag atcctaaagt agcgataaat aaaagaatca
     1981 gatataccca tgcatttgtt atatgaagga aatacatcag caagtacata catgaagaag
     2041 caagtaacgc aaatacgctt gcttcctttt taccaaataa ttttgtcagc tttgtagcaa
     2101 atggtgctaa aactactact gttccgtaag taaataaaag agcaacagac attgcttgtt
     2161 tattatggaa gtaatcattg aataaataag tcatattcgt cccagctaaa atctgattga
     2221 tcacaatgaa aatgtcaaca atgactaaaa taatcagtgc tcgattagct aacaaacctt
     2281 ttaccaactg agaagcagat actttttcgt ttttgacaac tcttactctt tcagtagtca
     2341 atttacaagt taacatatat gcgacaaatg ctattgcagc acatccgatt gaaatccaaa
     2401 agaaatgatt ccctgaaacg acttgttgtc cactcttgtt atgggtatac attaaaatcg
     2461 gaatgaagaa tcctgtagtt gatccgccaa tcgcagaacc aatgcttcgg aacgtggaca
     2521 acgaggtgcg gtcattagga tttgagctaa ttgctgctgc cattgctcca tacgggatat
     2581 tgattgttga gagaaaaatc ccataaacaa tgtatgaaac aaaaatataa gcaatcttca
     2641 ccgtcattgg aagatgttgg acgaatggca aaaacaaaat gacagtcact aagcaaaatg
     2701 gatacttcat ttttctgatc caaggaataa atcggccagt ttctgttaac tctgagacat
     2761 cacataatct tccaaccatc acatcagcaa acgcatccaa aattctcgaa atcaagaata
     2821 acgtacctac gattgcccct gaaacgccaa gaacattcgt ataataaatc atcaagaaac
     2881 tattgactaa acctaaaata aaacagttac ctagatcacc gaacatgtag cctatcttat
     2941 cggaaatccc aaattccttt ttttcttttt ttattgtatt tgctgtaaca gcattttgtg
     3001 aaagcatttg aattcctcct acctcttcta gtaaacgttt tcaaaattat aactcttttt
     3061 ttcttgtttt tatattcaca atgttggctt tttattgcat tatcttgaca gataaaaaac
     3121 aaaaaataat ctaaaaacat tgatgcataa gattgataac tttttttaca taacaatata
     3181 tacaaacaaa aaaattcact aactagtgaa aataagttag tgaacctcat ttattaaaga
     3241 attaaattac agatgatctt ttccatcata cttgaaaaac tcaaaatcag cataacttcc
     3301 tcttttttta tttagatcat gcactgctat cccgataaaa tttccagtaa accctcctga
     3361 gagtatacac atgttttttt gcggacctac tttcttccat tcttcagttg atacgtcttt
     3421 caaatgaaac tgtgctgtct ctttctttac agtgactttc aaaaaagtct tcttttctgt
     3481 gagtggaatc gtccaatctt ctatttgtgc tactccgttt tctgattcta tcatacgtag
     3541 gcattttccc ttgtcctcat tccaagtaac ataacaataa atataattgg attcatttaa
     3601 gtacagtacc agtccagcca tctgattaaa attattaggc atgtattcaa atgctgtctc
     3661 cgcatcaaaa gtaaaatccg tttgccgaat agctaacaaa tgatgttcga aggtagattg
     3721 aagagattca ccggaaatca gacgtaaaaa acctggtcta cattttgtat cacaccaatc
     3781 agcatttggc aaaatgcgtc tggcattcca gttcttcttt agctctcctt caaatgtatc
     3841 cataaactga tgatcttttt cttgttgtac ctcttcattt gtctcaatca ttatttcttc
     3901 catcggagtt gtctggctat ctttaagtct taaccaatcg tcggacgtcc aatacacttc
     3961 ttgaatagcg gtttctcttc ccaaaatagc cgcaccgttt aatggtctgg tacaaagata
     4021 tgtcatgtac catcttcctg acggggtttg tactaagctc ccatgtcctg tacattgcaa
     4081 tgtagaagca ggtttgtcac tagctgtcag cataggaaaa ccaggatcaa gctcgtaagg
     4141 accagtaata ttctttgctc ggcaaactgt aactgaatgt cctcttcctg taccgccttc
     4201 tgccgtcatc aaataataat acccgttacg aaaatacaaa tgtggtgcct ccgttttcgc
     4261 taattctgta cctgcaaata ttttatagat gggaccatca agcttcttct tctctgcatc
     4321 atattcttgt aatacgatac cgctggattt attcggtgtg tccagacgat aatcccatag
     4381 ttcattgaca agccattttt ttcccgtttc aagatcatgg aacaaagaag gatcaaagcc
     4441 gttactattt aaataaattg gttcggacca aggacctgtg atctcagatg cagtgatcaa
     4501 ataattgtgg acatctttaa acggacggcc tgcacttttg acatctgtgt aaacaagata
     4561 aaacaatcca tcagcataac tgatttgtgg cgcccatata cttccatttg taggatttcc
     4621 ttgcaaagat acttgagtgg tcaaaatatc cgttgtatgt gaccagttga ctaaattttt
     4681 cgactcatat actcggattc ctggaagcca ttcaaaagag gagacaataa tgtaataccg
     4741 ctctttcact cttaatatat taggatctgg attgaatcct cttaaaatag gatttttcac
     4801 tttgatagtc tgcatgattc aggctccttt tctcattctt actcgatata aactgaatgc
     4861 cccagctgcg aaataaaaaa atgcaaaagg aaacgcaata aactgagtgc caatcaacaa
     4921 tatgaatcct gttataaaat acaaaccagt ggcagttgtt aagtattttc ctttagcagt
     4981 caaaaacaat gctactgcta aaaaagcaag tgaaataaac aacacaacgg caaacagacc
     5041 agtcatgtcc tgtaataatc caaatgtatt ttctaaagac aaagttgaat gatctacagc
     5101 aagcgactga taactttttt taaactctga cgcagacata ttacttaaag taactgctaa
     5161 cccgccaaag aaaatcaaag atagactgcc accgaccatt cctagcaata gttcccattt
     5221 tcttttcatt tctgaacccc ctgcttttct aaatgtatcc attcaggtct tttgatcgat
     5281 gtttcataat tgttattacg ttcagagaat ttgacagtct gttcattttt cttgtcatcc
     5341 caattatccc aaccagttgg gtgaacatgc ccacctatct tacacgcttc aaataagaca
     5401 tttgcatacg gacgccacgg tcttcctaaa tagacgtttc tagtatcagg ttctgcagtc
     5461 aaggtacagc tttcaaaacg ataccctgtt ttttgatttt tcggcgtaga tgctgctgtg
     5521 atgtatccag gatttttact ggctaagcta tggatatgac actgatgaaa aacggcatct
     5581 gccccaccaa atatgaaatc caccgttcct tcaatccagc aatattcata ggtctgacga
     5641 caatactcag gattggcaga agccggtgta caaaaaggag taccatcttt ttgcaaatca
     5701 ggcaacggac ccgtgcataa ggtatcctga taaccagata gtcgacagtt ttttaaagat
     5761 acgcgatctc ctgaattgaa taaagcaaca gcctgtccta ccttttctcc tggacctgct
     5821 gtattttcga tgtgaagatt ttctaatacg acattcgatc cttctaagaa aaaagtagca
     5881 cttcggaaag tccctaactg gctaccgtca agatactcct gttttgcaaa acgattaccg
     5941 gagataacta ctgttcctgc tcccacaagt ttaaaattgg ataatctgct ttctatatat
     6001 tcatcatatc ttccttcttt tactatcaag acctttggat ggctatccgg caggcctgcc
     6061 aaataatcga ttccttcttg gatagtttca aaatccgtcg gggtatctat agcattcttt
     6121 ccaattatca gtttgtcttt catcctcata aatgctccct tatgaattgt ttccaaatat
     6181 ttctcatttc ttgtgtttcc atatgaccgc ctgttaaaga aaaagaagaa aagttttctg
     6241 gactacccat tgattgatac tttttgtcca gcccttttct cagcttcagt acgccttcgt
     6301 ccaagcacat cgtatcgttt tttccaacta agctaagacg agggcgcgga gcgatttttt
     6361 cttggattgc taacgttgta taagctgtca atagatttgg cacatagtaa taaaacccat
     6421 gatgatctag gcggcgatgt agcaaaagta tttcgatgtc cacctgtgcc gctagatcaa
     6481 tacaaacttt gacacgagaa tccaatgctg tcacccacca gcttaataag cctcccatag
     6541 aaagaccaat cgttgcgagc cgttgagtat ctgtgtcttc ccttgtctcc aaataatcga
     6601 tcaagctgat cacatcataa atacgcattc cccataacgt ttttcctgtt aataaaaatt
     6661 gtttgaacaa ctcgctttca gaaatacctc cgcgttcttc aaatccccaa gcatcaatcg
     6721 accaaacagc atatcccatt cctgtcagta cttctgcaaa agatggagaa gctaagtaag
     6781 gagcaccatg gatcaattca cttttcccga ctgaaaaatc tccgccgtga gaatgcaggt
     6841 aaaggaccgg cggaaacggt ccttcttttt ctggataagc atagagggct ggtactgttt
     6901 ccaattcatt taagtacaat tgatagtatt caagcataaa accgttttgt ttttctcggt
     6961 ataatcgttt tccactaggg gtatgtctgc tagatggagc acctaaaaaa tcattcaact
     7021 gcataagcaa agtttttacg aacaaccagt tcatccatat tttctgattg atttgattgg
     7081 aatttctcaa tactgatttc tttagtattt tctaatagaa atgctggtcc ctcgtgattt
     7141 tcaatcgaca tctggttaaa ggaaacctta cccgcaaagc caatataaaa tccttgtctg
     7201 ctcatttcct ctattcctgt catcatagca ggaacaccgg gaactgcatc ttgggccatc
     7261 gagacatcga tattgtcaaa tgtgatttct tgtatgaatt gttccgctaa tccataaata
     7321 aaacctgcag aagcatggac attccttgcc gtgatattcg aaaaatggat ccttctgaat
     7381 gctggtgtcc gttcatctat cggataagcc tttttttccc atacatacgg ttctttccct
     7441 cgcggaccac agaaataata aagattcagt ataaatggac acatcacatt atccatgact
     7501 atattggata ctcgaatatc ttcaacgatc ccgcctcgac ctcgtctaga tttcaatcgt
     7561 attcctcgat cagtttcttg gaagatacaa tttgaaatgg taatattacg aatactacca
     7621 ctcatttcac ttcctaatac gacaccgcca tgtccatgga ccatcgtgca atttgtgatc
     7681 gtgatatttt cgcaagcgat cctttcgtac gtatcttcag tccctgcctt gattgcaata
     7741 cagtcatctc ccacgtcaat atggcaatta ctaatacgca catttttgca tgattctgga
     7801 tcaattccgt ctgtatttgg tgaatccgct gggttcaaga tcgtcaagtt atcaaacgtt
     7861 gcattactgc aaagaatcgg gttgatcgtc caacttggtg actggatcaa tttaatatct
     7921 ttcacagtaa ttcgatgaca gttatggaaa ctcatcaatt taggacgagg ataagcgaga
     7981 ttatctggtt catttctaaa agtatgccac catttttttc catttccatc taatgtaccg
     8041 aaaccagtga cagagatgtt ctctacattt tgagcataga tacaggaagc ataaactttt
     8101 cgatggactc cttcccaacg tgaatgaaca acaggataat cttttggatc gtcagagaat
     8161 ttcagaaccg ctcctgcgct taaatgaagt tcgacattgc tttttaaaaa taaagctcct
     8221 gttagaaatt cacctgctgg aacaactacg gtcccgcctc cgtcacttgc tgcagcatca
     8281 atggcttgtt gtattgcttc agtattcaat tcatcaattg atgcgccaaa tgtcaaaata
     8341 tcatacatat ttataatgcc tccacttcaa tcaatgcttg caagaatgca ccaacgcctt
     8401 tttgatcatt acaaataatt ggttcactga tgtagtaagc atatgaacca tctcgctgat
     8461 ctgctccgcc taaccctgct acttgacagt ttttattcaa gttcagccag ccttcttttg
     8521 tttccagaac aaattcatca agtaatcctt gatgtgattt cttcacttgt ggaagccatt
     8581 cttctttgtt cagcagtccc aaacgtattc ctttagccat tgcgtaaaca aacatactac
     8641 tgccggaagc ttccagataa tttcctttcc gttttgtttc tgttgttact tgataccatg
     8701 tcccgctgtt ttcttcttga tatggaagta atgcagccag tgtcttttct agaatttctt
     8761 tcagcaaaga acaatctgtt tcttcatgaa gtaattcata ggtatctgct gcagccatca
     8821 aataccagcc aatcgatcgg ctccaaaaat gatgggatag acctgttttt ggatcagccc
     8881 atggttggac cttcttttca tcccatgcat gaatcagcaa atttgttttt ggatcgaata
     8941 gatgtttata actgatttca aactgtctca aaacatcttg gtaatctttt tcttcactaa
     9001 aattctggat aaattcagca taaaaaggag aacccatata taagccatcc aaccaaattt
     9061 gataaggata aatttcttta tgccaaaaag ctccttcgga tgtgcgtgga tggtttgcca
     9121 actgctggga aaggagttct gctgcttttt tatatttttc ttcgcctgtt tctttgtaca
     9181 ataaaaagaa aagctttcct gtatttacat ggtcgatatt gtattcttca acgctgtagc
     9241 cacggatcgt tccatcttct tgcacgaaat agtcgatatt gtcttttatg aaatcaaaat
     9301 aaattttttc ttgggttcgt tcccaaagta attggaaccc tttaagtacg acgccatagt
     9361 cataagacca ttttccttta taccactttt cttcatataa gcgaggtgtt ctttccatga
     9421 tactttttgc taatttcttt gaccagtttt ccattatttg ctctccttta aaaattaaaa
     9481 taggatttgg gacaaatacc aagaagtatt tgtcccaatc gtctatatta ctgtgccgct
     9541 gctttgtatg cttcctcgta ctcttgtgtg attcgatcgc ctccttgtgc tttccagtct
     9601 tctacagctt ttttgaatcc atcttcgtca agctgtccca tgatgtattg caccgtagca
     9661 tcaatgacga ttttttcaat ttctgatcct acttcattgt tcgtcggtga atccaatggc
     9721 acagaaggat ccattacagc aaattcttca ttttctttga tcaattgatt agagaattct
     9781 ttttcagggt ctgtatcttt gaaagagaac gtcacttcac taggacgaga gcttgataac
     9841 ggttgcacct cttgttgcca aagatccata tcgataaatt cgatagaacc atcatcattc
     9901 aattcataat gcgttccttc gataccgcca gtcatcaatt tatacacatc ttcttctaaa
     9961 agatcatcaa caaattgaag taatttcttc aattgtgctt cgtcttttac ttgagatttg
    10021 ggaaatgcta gcaagccgcc aataccattt ccttctgacc aaacatgata accgccgcct
    10081 tcaccatctg agatcttgtt tacaggcact aattccatat catcttgaat accttgaccc
    10141 atcgtcttca gattcttgat atccaccatt ccagtgtaga ttccagttct tccttgtgca
    10201 aattgttctt gctgatcagt cttttgggta actgcaaagt cttgtgccaa atagccattc
    10261 tcaaatagat ctcttgaaaa attcaaagct ttcatatact cttctgtatc aaattctgga
    10321 gtgaatttgc cattatcgtc tacagcccag ccatttggtg ctccaaaata agaagtgaaa
    10381 agtttaaagc tggtataacg aatatcacta ggcgcacgat cgccaaaacc aactgtgtcg
    10441 tccacaccat ttccatcagg atcgttttct gtaaattgtt tagctacttc ataaagctca
    10501 tccatggtag ttggaacatc tagtcctaga ttatccaacc agtcttttcg aatgactaag
    10561 ccagcacgag cgtagtcttt ttgtatcggt acgccgtaca aggcaccgtc gatactcgca
    10621 gcttcgattc tatcttcaga aatttgactt aaatttttat agtcttttaa atattctgat
    10681 acatcccaga atacgcctga ttttaacgca ttgcggacag aagagttttt caacatcgca
    10741 atttgaagtg taacgatatc tcctagttca tcagaagcca atgcagttgt taatctttct
    10801 tcttttgaag catcaggaat ccagttgaat gtcagttccg tatcggtttt ttcttcgatc
    10861 aagttcacaa tcgtgtctgt aggcggtgaa gcatgatgca aaatatttaa ccaagtgatt
    10921 ttagcttttc catcttctgt ctctgaagaa ctgttacttg aactgctgcc gcatgcggca
    10981 agtgctagaa ttcctgcaaa actaagacct aattttacga gcgttgcttt tttcataaat
    11041 tttctccttc actgttttaa taatattttt gttttcttct gttttttctg tatatgagtt
    11101 cttccattcc aaatggcaaa atatagaatt tgctgtccac gtgactgcgg aaaagaagag
    11161 actgccaccg ataaaggtta aaacacctgg cattgtttgg atgatcatca gataaatccc
    11221 taccatactt aatatcaaca ctaatgagta atgaagatgt gagaatccga ataaaaatgc
    11281 cattttcatt ttgaaaaaat atcctttcca atcataatga gccatgattg ggaaaatata
    11341 gagtgctgag agcacatata tgaacgcagc aatatacaaa gcgatcctaa gataccaatt
    11401 gtgtatgaaa gaaagatcca caagtaaaac aatcccgatg gcgccatata tccagctcat
    11461 cagcaaggtt tcacgatagt tttctttaaa atatgtgaag tacgttttcg tcaaaggaaa
    11521 cgtttctttt gagcgcagcc attttctaat cactgcaacc atcgcatacg atgcaggacc
    11581 gaaagtaaaa actcccaaac ctgcgatgac taaaccgccc cacagaaaat tcaaataaac
    11641 caactgtaaa atacgattta gccttccgtt tattttatct aatgactgca gcatatcttt
    11701 ccctctcttt ctatcttagt aaacgccgtc ttctcctagc tttttggcaa tcttattagc
    11761 taaaattacc ataaacaatc ctacgacccc tttaaacaat cccattgctg tactgaaact
    11821 taactgtcca ttcttcaatc cagctgtata aatataggtg tcgaaaattt ctgcaacgct
    11881 tcgattcaat gaattcaata acaagtacat atgctcgaac cctaagtcta atgtctgacc
    11941 gatttttaga atcagcaagg taatgattac tggacgaatt gccggcaatg taacatgcca
    12001 tgttttgcgt aagcgtcctg ctccatccat ttctgcagct tcatacaact gtgtatcgac
    12061 tgcagttatc gctgaaaggt agatgatcgt agaccagccc aactctttcc agatcacttg
    12121 tccgatatat actgtgcgtg tccattcagg ggatgttaga aaactgattt tttcaaatcc
    12181 cattgttgca aggaattcat tcaatacccc tccgtcaaca ttcaaaaata cataagtaat
    12241 cgaaacaatg attacccaag acatgaagtg agggatgtaa atgatcgttt gaatcccgtt
    12301 tttcaatcgt ttgttcgtta cttcatttaa tagtaaagac aaaatgattg gcatcggaaa
    12361 aaacacgaag atatttaatc cgaacaagat caacgtattt cttaataatg taaagaatgt
    12421 cggttctgta aacaaacgaa tgaaatgttt gaaaccgacc caaggacttc ccattattcc
    12481 taaaaatggt tgataatctt gaaaagcaat aacgagtccc cccataggaa gatacctaaa
    12541 aatcaagaaa taaaggactc ccggcagaat catcacatat aaaaatttat tcgttttgat
    12601 tcctgcaatt attttttttc gtcttctgag cttttcttct tttttcgata gcgtcagttg
    12661 aaatggttct tgtttcttta cactttccat aagcccctcc ttagttttgt gaatgtttgc
    12721 tgcttatgac ttcatgatag ataactatct gaattacgta tataatcatt tttaccgtaa
    12781 gtaaaaacgc ttacatagca atgatttttg tgctatacaa aacgataaag tgtatttttt
    12841 cacaaataaa ctacgcatag caaaccttct atttttttct atctgacact tagcatagga
    12901 aatgattata tacgtaatct ttcaccgatg ataaattgaa catatagaaa cagaaacatt
    12961 taatttattt tgaggaggag cctatctata tgaaaaagaa agtctctata ggagaaagaa
    13021 gttttacact tttcaacaat acgtttctaa ttttgctagc tttgatctgt atcgttcctt
    13081 ttttaaatat catcgctaca tcttttgctt ctacacaaga agtcgtcgcg aaaaagttta
    13141 tcctttttcc tactaccttt tctttagacg cgtatcgcta cattctttct acgccgacca
    13201 tttttagagc acttgctgtt tcgattggtg ttacaggtgt cggaacgatc gtcagtatgt
    13261 gtgcaacttc actgatggcc tacggtcttt ccagaaagta tttgtttggt cgaggtttcg
    13321 tcaactttct tgttgtattt tctatgttgt tcagcggggg gatgattcca acgttcttag
    13381 ttgttcgttc tctagggctg gtcaattctt actgggccat gatcctgcct gtggctgtta
    13441 atgcaatgaa tatgatcatt atgagaaact ttttccaagc cttgccggat agtttggaag
    13501 aatcagcaaa aatggatggt tgcacagatt ttggtgtatt tttcaaaatt atgctgccat
    13561 tagcgttacc gtctattgct actatctcgc ttttttatgc tgttacttac tggaacactt
    13621 atatgactgc tattttgtat atcaacgatt catctaaatg gccgatacag attcttttac
    13681 gccaaatcgt cattgtatca agtgggatgc aagcagaaag tagtgccgtt gatgtgattc
    13741 caccagcaca aacagttaaa atggctgtta tcgttattgc aacagtgcct atgctgattg
    13801 tttatccttt tgttcagaaa tattttgtca aaggagcact cgtaggttct gtcaaagggt
    13861 gattatgtac tgaacgcaaa atggtagttg tgtcagaagt ggtcagcttc aagaaataag
    13921 aaggcattta cgaaaattgc ttcttaaatt tttgtgaaat ttcggcttat ttttcgaaga
    13981 atctactcct ggaacaccgt gtatcagagg ttgtgaaaga gacgttcagc cttgagcgat
    14041 gataaaaact ctcacaaaca ttgcgacgag taacccacag gagcaataag aagcgatttt
    14101 gaaaaatagc ttcccatatt tcacaaaatt atgccttatt gtcgatagcc tgtctcttga
    14161 acgccattta ttcgtaaaaa agaactgtga caagactttt gtcacagttc tttttgcgtg
    14221 cttagccttg aaatttaact atccataaat acaaatacta gggttattct aacttctttt
    14281 attgcttgaa tcagtccaca agaaaaacag cctcttgatt cacaaatatt tttttcgata
    14341 ttctcccggc gtaatcgatt ccttcttttt aaagaaacga ataaaatttt gcggatttcg
    14401 atattgcaat ctttctgcaa tctcttttac ggagaggtta gtctctttca accatttttt
    14461 tgctacctct aaacgatgat tttgaacaaa atcaccaaat ttttctcctg tttccttttt
    14521 aaatacatta cttaaatagt tagggttata atgcaaccta tcagcgatca catctaaaga
    14581 cagatcacga tcatattctg tgtagacgat atgcataata ttctccgaca aatttttaaa
    14641 ttcatgatca gtgattgctt gagagcattc tgtgattggc agaactaatt gttctaaaac
    14701 aagtttttct agttctttgg gatgataatt gttcagtgct tttacataaa tttgtttcat
    14761 gttatcaaag ttttttgaat ctactcccaa taactgtcct aattgaacga gttcattgat
    14821 caagcggacc gaaactactt cccgactcaa aggattttta ttttgtgcaa acaatgcatc
    14881 aatcaattca tgtactaaat tcttcacttc ctgatttcca gaacgaatgg cttcaaacaa
    14941 tcgattttgt tcttctatcg gatatctaat aagtgttttt tcatgagcag caggaacgat
    15001 ttcctgataa aaaataattg aacttggacc agtatttaca cgataataca gggcttcttt
    15061 tgcccgatca accgattgct tgctttcatt cagcgtttcg aaccggttac tgatgcctat
    15121 actgatagtt aaattcaagt attcttttac acgtttttga atgagtcggc aatactccat
    15181 gacttttttg cttgcttctg catcatcttt atccattcga taaatagtgg cttgcatttc
    15241 ttcattcaaa acgatcggca gcattcgatt ttcctttggc acaatttccg tgatcatgtt
    15301 gttgatcgct aaaagtagca aatcacgctc cccaccgtga ctatcttcta acagatcgat
    15361 ttgaaccaaa gctgtataat agcagtcatt ttgagaacta taaccaaatt gctgcaaccg
    15421 ctgatctagt tctcgccttt ctactcgatt tctaaataaa ctaagtacaa atagcgtttc
    15481 cagctgtggc ttttgggtaa ctaagtgtgc acttaacgct tctttttctc ctatgatccg
    15541 actgactgat tgagcgacca aatctagttc tttcccactg aatccttttg atttaagatt
    15601 cagacgttcc tgtatttgag aaatcggacg ggcaaatctg tccgaaaata agtaggaaag
    15661 aagaccaatg agtcctacca gtgttaaaga cactgcaact agcccgattc tcagattgcg
    15721 gatcattgcg ccaattgcac tagagtcaac ttctaatctg taaatccaat tattataatc
    15781 tgatttcagt actatatacg ttttatcttt ttctcttatc actttcagct gattgtaatc
    15841 tagatccacg tccgttaata cagttggttg ctctttattt tttactgtct gagtagtaaa
    15901 aaggttgcca acttcgtttt caatagaaag tatgtcttct ccactttcac caacaacttg
    15961 ttctattgta tgtttctcta tcgtagcaat tcccaatgca tatttttctt tttcaaacat
    16021 tggcaaagaa atcaccattt cgattccttt gtttttcttt tgccaaaata aattattttt
    16081 gttatttatg tacttctgtt gatacttttc aacttcctct tcggtcagtt gactcaacga
    16141 tccattgatt actccccatt taccagccaa actgactaaa tcataattat tatcatccat
    16201 aacaatcgtt tctataaaat ttagttcttt cctgatttca gaataagtgg agtaatctgt
    16261 atatgtcagc ggatgcttga atatgtccga gaaatttttg gtcgaaccat acgtagaaaa
    16321 cgaatattca atcgttctaa ttttccattc aacgttagtt ttaacttgcc tgagcaaatt
    16381 ctttttgtct gttaaataac tcgattcgat cgattcatag gtattctggt aaatgaataa
    16441 actaaatcct atgacaagta cggctcctaa taaaaacatc ggtaaaaata ttttataaaa
    16501 tactgtactt ttcttcattt tcttcctctc ctccttttaa aataaaaggc tttcattttt
    16561 ctgtaaaatt gatactcatc taattaaatg agcattcccc gctttcaaga atagcataat
    16621 taaaaataaa aatccaagta atttttaacg tgtgcaatct tttcatatca aagatctatt
    16681 gacttatcgt agttatcgta atatgatgaa tgcgttaaca aaaagtgaaa aagtaaggaa
    16741 aatgatggaa tggtaacgat caaagatatc gctaaagcag ctggcgtttc tcatacgaca
    16801 gtttctcgtg ctttgaataa cagcagttta ataaaaaatg aaacaaaaca aaaaatacga
    16861 caactagcta aggaaatgaa ttattcgcca aactatagtg caaaaggttt agtgaatcaa
    16921 aaaaaatatc taatcggact atttttctca agtttgcata aaggaacttc atcaaatttc
    16981 ctagcagatt ccattgcagg catccattca gtattagaca caaattacag tttatctgtt
    17041 gaaagtatcg atgttgttga tttaacagaa atcaacctgc aacgctacga tggactgatc
    17101 gttatgagtc aaagtgatga tgatcagcct ttcatcgact atctcaaaga aaaagaattt
    17161 cctttcgttg ttgtcaatcg tcacatcaat gatccagcgg ttatcaacgt cactgccgat
    17221 gatgcggccg gtgttatgca ggcagtagat tacggtattg gatgcggaca tacgaagatc
    17281 gcttatattg gcggaaagaa aaattttcgt tcgacaaaag aacgggaaaa aggggtattg
    17341 aaaagtctaa aaaaagcgag catccctgtc aatccagcct attttttaac aggagattac
    17401 agcttagaaa gcggcctcca aaacatggaa tacctattat cactttctga cctgcctact
    17461 ctggtttttt gcggcaacga cgacatagcg attggtgcat tgagagctgt acatttagct
    17521 ggcctccgtg ttccccagaa catttctctg atcgggttcg atgattctcc agtcgtctcc
    17581 tatctcaatc cgccgcttac caccgtacgt aagcctttga aagaaatgtg tcaagaagga
    17641 acaaagcttt tactagattt gatcgaaggc aggacgatcg aacaatctaa aattgagctc
    17701 tccaccttgt tgatccttcg tgagtcgatc aattacttgt aaactacaag taattttata
    17761 taaaaatgtt aacgtgtgca taacacagaa aggaagttta tatgaaaaaa ctgacaaaag
    17821 aattagtgaa aaaaaacgaa gcacctgtaa aagttcttca gtttggtgaa ggcaatttta
    17881 tgcgtggatt tattgattgg cagattcagc agctgaataa ccaacaattg tttaaaggta
    17941 atgttgcaat cattcaacct ttagaacaag gtcttggaaa tatgatgaac cagcaagata
    18001 atttatatac ggtcatttta caaggactca gcaatggcga aatcgttgat acttccgaga
    18061 ttatcaccgt agtagaacaa atgatcaatc cttatgatga atgggatgct tatctatctc
    18121 tagcagaaaa tgacgacttg gcctatatcg tctccaatac aacagaggca ggaatccaat
    18181 tcgtggagac tgataattta acagatactc ccccacagag cttcccgggc aaactgacag
    18241 cctttttata ccgacgtttc aaattaaaca aagctggctt taccatcatt ccttgcgaac
    18301 tgattgacag aaacggcgaa cgtctaaaag aaatcatttt acgctacgca gaaaaatggg
    18361 atttagaacc tgcttttgtt gagtggttag aaaaagaaaa tgttttctgt tgtagtttag
    18421 ttgatcgcat cgtcccaggt tatcctcgcg aagatgcagg tgttttcaat caacggcata
    18481 actatgaaga tcaattgatg gtcaaagcag aaccattcat gctttgggtg atcgaagggc
    18541 ctaaagaatt ggagaatcaa ctaccattca aacaggctgg cttgaatgtc atcatcacag
    18601 atgatatgac tccttatcgt gaacgtaagg tgcatttgct taatggaccg catacagcga
    18661 tggtccctct tgctttgttg gcggaattgg aaactgtaga agaagtaatg aaagatcctt
    18721 tattctccgc atatgttgat cagttattca atctagaatt gattcctatg ctttctttac
    18781 ctaaagatga attagccatt tatgctgacc aaatcaaaga acgtttcttg aatccttttg
    18841 cccatcacaa attagaagcg atttcattaa atagtgtttc aaagttcagc acccgtctgc
    18901 ttccagtgtt caagaagtat atcgaagaac aaaatcaggt accaccgtta atcactgttt
    18961 cactagctgc gctacttctt atgtatcgag gcgatcaagt aaagccacat gatgatgaaa
    19021 agacgataag cgaatttact gatgcttggt ctgataatgg aacagctatt cctcgattat
    19081 tgcaaaacgc tgcgctttgg ggagaagatt tgtctcaaat accgaatgtg acagatacag
    19141 ttcaagaaat agctgcaaag atcgatcaaa aaggtgtccg gtcagtatta caagaaatga
    19201 aaggaacgat ggtccatgct tgaaaccctg caaccaacaa tgaaattaaa tcgacgagac
    19261 agtgtggtcg tcgcattaac ccctattcca aaacaaacac aactgacgat tgatcagcaa
    19321 acgatcacca ctcttgaaga tatcccgcaa ggtcataaga ttgcattggg tgatttgaaa
    19381 gctggagaca acgtgatcaa atatggctat ccaatcggtc atgtgacgac agacgtaact
    19441 gccggtcaat ggctgcacac tcataatgta aaaaccaatc tttctggtga attggattat
    19501 cgatatgaaa aagatgtcca tccaagtcat tacctttttg agaaccgaac atttcaaggt
    19561 tacttgcgaa aaaatggaaa agtagggatt cgtaatgatt tgtttttagt acctaccgtt
    19621 gggtgtgtga atggcattgc tgaactgatc gtcaaacagt tcaaggaaaa acatccagat
    19681 ctgggacagt ttgaccatat tacgatttta aagcatcctt atggctgttc gcagcttgga
    19741 aaagatcatc aaaatactcg agaaatttta gcggacgcag taaatcatcc caatgccgga
    19801 ggagttttag tatttggatt aggttgtgag aacaatacag ttcctgaatt caaaaaaatt
    19861 ttaggtgaat atgatgaaga acgagtgaaa tttctagtgg ctcaagatgt ttacgatgag
    19921 atcgaacaag gtgtggctct attagaagag cttttaacag cggcggaaaa tgaccagcgc
    19981 atatcagtcc cgctctctaa gttgaatgtc ggtttgaaat gcggcggttc tgatggtcta
    20041 tcgggaataa ctgctaatcc tttacttggt gcattttctg actacttgat tgctcaagga
    20101 ggcagcacga tcttaacaga agtgccagaa atgtttggcg cagaacaagt attgatggca
    20161 cgagcggaaa atgaagaagt ttttgactca atcgttgact tgatcaatga cttcaaacag
    20221 tatttcttgt cttacgggga accagtttac gaaaacccat ctcctggtaa taaagcaggt
    20281 gggatcacta cattagaaga taagtcttta ggctgcacgc aaaaatcagg aagctctcct
    20341 gtcgttgatg tgctacaata tggcgaaaaa atcagaaaac cagggcttag tttattgcaa
    20401 gctcccggga atgatcttgt agctgcctct gctcttgctt catccgattg ccagttggtc
    20461 ttgtttacca caggaagagg gactccgttt ggctcatatg tcccaacggt caaggtatct
    20521 acgaatacaa ctctttttga acgcaaaggt cattggatgg attttaatgc aggcgtctta
    20581 ttagaacaac caatggaagt aatcttagaa tcgtttgttc aaaaaatcat cgctgtagct
    20641 agtggagaag aaacgaaaaa tgaacaaaat gacgttcgag aaattgccat ttttaaaaac
    20701 ggagtaacac tttgattttc ataaaacagt ataaaaagaa agaaggaaga tttatgtttt
    20761 tatctgatga ttttttatta aaagacgatt gggcgaaaaa gctttaccat tcctacgcaa
    20821 aaaacatgcc cattattgat tatcactgtc acttagatcc aaaagaaatt tacgaaaaca
    20881 acaatttcag caatctgaca gaagcttggt taggaggcga tcattacaaa tggcgcttga
    20941 tgcgagcttg cggcgtgcct gaagaaaaaa tcacaggaaa tgcttctgat tttgaaaaat
    21001 ttttagcttg gtgtcaaacc gtaccaaaaa ttatcggaaa tccgctttac agttggtcac
    21061 atcttgaatt gaagcgtttc ttcggtattg acttgttgat aaatgaagaa aatgcagaga
    21121 aaatttggac acaagcaaat gaaattttgg cagctcctga ttttcgccgt cgggaaatcg
    21181 caaaaaaatc gaatgtagaa gtgatttgta caacagatga tcccgcagac gacttgcact
    21241 atcatcagtt gcttgctcat gaagaaccag atttgaaggt tctaccttca ttccgtccag
    21301 ataaagcact caatattgaa aaagaaggat ttaatgcatg ggtcacacta ctggaagaag
    21361 cagctgacca aacgatcacc acctatcaag aactgataga agtcttaggg cagcggatag
    21421 aatacttcca tcaacatgga tgtcgtgttt ccgaccacgc tttagacatc ttacgctata
    21481 aagaagcgga tgaaacagta ttagaagaga tttttcaaaa agcacgagct aacaaactat
    21541 tatcttcaga tgaaatcgat gcttatcgta cagaaacact gatacatttg acccacttct
    21601 atcattctca taattggacg atgcagctgc acatccatgc ttatcgtaac tgtaatacac
    21661 aaatgttgga aaaattaggg cccgataccg gctatgatgg aatgaatgat ctttctttga
    21721 ctattcctct gcaaaaatta ttgaaccgtg cagaagaaac ggatcagttg ccaaagacga
    21781 tcctttattc attgaatccg aatgactatc ctgcgatttt agctttgatg ggctgtttcc
    21841 aaaaagaaca gaatggaaaa ctgcagttag gttctggatg gtggtacaac gatactcgag
    21901 ctggtatgcg cgatcaattg acacaatttg ctgatggtag tgctttgggg aattttgtcg
    21961 gcatgctgac agattcaaga agtttcctgt cctacactag acatgaatat ttccgtcgtg
    22021 ttctttgtga atttttaggc gaaatggtca tgcgaggaga agcacctgaa gatgaacaac
    22081 ttttaggaag tctagttcaa gcgattagtt atacaaatgc taaaaattac tttggctttt
    22141 ttgcctaatt gattaatagt aacagtaacg aatcaaaaac aggatagtga aataacggaa
    22201 tccgtttatc cacgttttag agaagaagac ggaccaaatt tgctccgtct tcttctcttg
    22261 ctgttctgct tcattgccta taaaccttta tgaaagaaga gataacatgc ctactatcca
    22321 tattgccggt gattctactg cggcagaaaa agatgaaaac aagcgtcccg agacaggttg
    22381 gggagaaaaa atctctgctt atttttcacc cgagatccag atcaataacc aagcaaagaa
    22441 tggacgcagc accaaatcat ttatccagga aggaaggtta gcccgtctag cttcacagtt
    22501 tcagccgaat gatttcttat ttgtacaatt tggacataac gaccagaaaa tccaagaaga
    22561 aagaggtacg cttcccttcg gtagctattt aaaaaatctg cagatatttt ttgaatgcgc
    22621 aaaaaaagcg aacgtccaaa tggtcttact gacatctgtg acgcgacgag attacttaga
    22681 aaacggaaca ttgaatccag atatacttgg agactatcca aaagcgatgc gtgcatttgc
    22741 tgagaaacac catattcctc ttttagatgt gttctcaaga tcacaagaat tgttccagac
    22801 cttctcaaaa gaagagacaa aaaaattcta cctgcatctt atacctgata cttgtaaaaa
    22861 ttacccagaa ggactaaaag acaataccca ctttagtcca gaaggcgctg ataaagttgc
    22921 ccgaataatt gttgaattga taaaagaatc ccgtctgcct cttgctaatt atttacaaaa
    22981 aggagtttga gatgtttcca ttaaatttag gcatccgtgc ccacgattta gatgttcgaa
    23041 atcgtgaaga aatcgttcaa aaagtatcac agcttcactt gtcccatatc caatttgcac
    23101 cacaaaaagc atttcctgaa caacttaaat taactgacat gacacttggg agtgctgcct
    23161 attttggtga atacttccaa aaaaacaata tcgaactttc cattttagga tgctatatca
    23221 acctatccag caaaaatcaa gaagttcgtg cacaagcatt ggcaactttc aaacagcaaa
    23281 ttgctctgtg ctctgcctat caagccaaga tgattgctac ggaaaccggt agtgtctctg
    23341 tcggttacac aaaagaaaat tttactgaag aagcttatca gatcgccaga aattctatca
    23401 ttgaattagt cgctcatgca gaaaattttg gcgtaactgt tgctgttgaa gcagggatca
    23461 atcatccaat ctataactat cagttagcta aacgtttaat cgatgaagta gcttcaccaa
    23521 atttaaaaat cattttagat tgtgcgaatt tgatgcataa agaaaatcat gctgatcagg
    23581 aaaaagtgat ccgaggtgcc ttggacaatt tacatggtta tattacagcg cttcatctca
    23641 aagactatat catggaaaat ggaaagatcc gcatggtacc tgttggcaag ggctgtttag
    23701 atttccgccc ggtactgcat tatatcaaat atgagcgtcc tctaatgtac gccactcttg
    23761 aagctactcc tgagatttat gttcctgaag caatagaaca cttgcagttt atttaccaag
    23821 attgttaagg agaagattat ggaggaacga atacagaaaa gttatcaaaa atatcatgca
    23881 aatgcttttc agacattgct gcatctttat cagggaaatt attggcgttt tgccatgtca
    23941 ggactctttt ttattatcaa acatcttcca tcttggttaa tgccgatcgc tattgccaat
    24001 gtaatcaatg cagcttcatc gaaagattct tctcagttgc aggccattta tttcaacgct
    24061 tttttgatgg cttgtctgat tggtcaaaat atattgacga attattttca tgtaaaatac
    24121 catagcactt cgatccgtca agtagaagct ggactaagag cgacccttat caagaaaatc
    24181 caagaactat ctattcctta tcaaaaagaa ttgcaatctg gacgaatcca gtcaaagatc
    24241 atccgtgatg tagaagctat ccaaacgttg tcctctcaag tatttgtcag cggattaaat
    24301 atcgctgtca atttattcgt agctttaggt atcacgcttt ataaaagtcc tgttgttttc
    24361 atgtttttct tattgactgt tcctctcgcc gcattcgtta ctctcttttt ccgtaaaagg
    24421 atcagacagt ccaactcaga cttcaggcaa gaaatggaag agacttctgc aaaagtgatc
    24481 gaagtgatcg aattagcacc gattgctcga gctcatgggt tagaaaaaca cgaaacaact
    24541 cgcttaaata agcattttac tcaaatctat gaaaaaggat tccacttaga tatcctgcaa
    24601 tctgttttcg gatcagtcag ctgggctagt tttcagatat ttcagctgat ttgtttgatt
    24661 ttcactggga tattagcttt acaagggcgc atccaacccg gcgatgtcgt gatgtatcag
    24721 acgtatttca cgacgatcgt caattccgtt tctggaatga tcacgctgat tcctacgatt
    24781 tcaaaaggca tggaatctat cacttctatt ggcgaactgt tgattgctgg cgatgtagaa
    24841 acttatcacg gtaagaaaaa aatccaacat ttggatggaa attttcgttt tgaggatgtt
    24901 tcttttcttt atccggactc actcaaaccg attatcgatc atttttctct agaggtgaaa
    24961 gctggagaaa caattgcttt tgttgggcca tctggctctg gaaaatctac actccttaac
    25021 ttattgattg gatttatcca gccgacttca ggaaaaattt ttctagatga tcagcctttg
    25081 gatgaactgg atatgcgaag tgtgcgaaat tatttagccg ttgttcctca aaccactttg
    25141 ttgttctccg cttcgatcaa agagaacatc acttatggct tgaaaaacgt atcaaaagaa
    25201 cgtttagatg aagtcatcga agctgcgcaa ctttcttcac tgattgacca gcttcctgat
    25261 ggtctggata ccttagtagg cgaacatggc aacaagctgt ctggtggcca aaagcaacgt
    25321 atctccattg cacgagcgtt gatacgtcag ccaaaaatca ttttgctaga tgaagcaacc
    25381 tcagctttag ataaccagtc agagaaaaaa atccagcaag ccctgaatta cctgacagaa
    25441 acacccacta cttttattgt ggctcatcgg ctttcaacga tcaaagaggc agataaaatc
    25501 gttgtgatcg atgaaggaaa aatcgttgaa ataggtactt acgaaaaact gttagctcaa
    25561 aaaggctatt tctaccgctt gaaaaatgaa tgacaaaaaa taggagctat gacaagactt
    25621 ttgtcgtagc tcctatttct gaataaacgg tgttctcctg ccaactcctc ggtcacaagt
    25681 cacaatagtc atcaaacatg agaagctgtt ttttgaaatt gttccttgta actcagagtt
    25741 aaacggtagg ctcacgacct cttggtaaat ggtgttcaag agacagccta tcggcaataa
    25801 gaagcaattt tgtcatcgct caaggccgaa cgtctctttc acaccctctg ttgtttcata
    25861 aaatagtttt attcgctgta aaggtcgcga actggtgtca tgatgatacg gttcaagtca
    25921 ttgatgattg tactgaagcc ttgctctttt tgcatcaagt cattgattac ttcttctgat
    25981 tggattttca cagccatttc ttgtgctttc attgcatctt cgtcgctgaa ctcttgtcca
    26041 ctcatttgtt tttcttgtaa ttcgatttgc atcgcttgga agtctttgaa cagtttgtgt
    26101 gcttcttcgt ttgcttgtac tttctcgtac gcttctttca acgctttgaa ttctggtaat
    26161 tcacggatct cacgttcgat ttgattcgcg ctatcgtaga tgtttgacat gttatgccct
    26221 cttttctttt agtagttact ctctattgta acatggggtt attcgttttc aaaggggcta
    26281 ctctccttga tatttccgcc agaggtcttt agccgcttca ccaattccct gaacgccttc
    26341 tttcaccata tcgccgaagt ttttcagccc ttcttttgct tgttcaccat attgattcaa
    26401 gttatcttgc caatttccag actctccatt agaatttcct gtgtttccaa cttgatctgc
    26461 agctaccact tgtcctcctg tggcataggc atctgctaca ctaaatcctg tttttgaagt
    26521 atacggaagg atgccttctg ccgctgattt gaagactttc ccgacaacat ttccacttgt
    26581 cccttccaaa tagtgcagct tgctggattc ttcgtaaccc atccatgtcg aaatcactac
    26641 gtctggcgtg taaccgatca tccattggtc atttgctttt gtggcatcga aattagtttc
    26701 tgtggttcct gttttccctg ctatcgtata acctgcagga ttcgcagcag ccgctgttcc
    26761 atttgaaaat gtaccaagaa gcatgctggt catttcatcg gctgtttcct ttgatatgac
    26821 ctgttttggt tttttgtttg tgttatcgac gatcaccgct ccagtcgaat caacgatttt
    26881 cgtgataaga tgcggcgtat acatctgacc atcattggca aatgcactgt aagcacccgc
    26941 catgatcaat ggagatactc ctttttccag tccaccaagg gctaagccat aatacttatc
    27001 agattttgct aaaggcaggc cgaattcttc tgctttatca tatccttttt gtaaaccgat
    27061 ctcatgcaaa agccatacag taggcaaatt caaactttgt gcaacagctt gatacatcgg
    27121 tacttctcct tggtatgtcc cgtcaaaatt atgcgcatcg taataacttt gcggttcgtc
    27181 tttcaagata ctggaaggct tgtatccagc ttccagtgct ggtgtatata cgcttaatgg
    27241 tttgatcgtt gatcctggtg aacgacgcat ctgtgtagca aagctgaatc ctctaaagac
    27301 atgatctcct cttcgtccta ctagtgcttg tacaccacct gtattcggat ctaaagctac
    27361 tgaagcagat tggaccatgg caccatctga agcatttgct gggaataatg cactgttttg
    27421 ataagtagct tctaattgct tttgataatc ctgatctaaa gctgtataga ttttatagcc
    27481 tttattcatg acatcttctt catcaagacc atactcgtta actgcttcat cgatcactgc
    27541 atcaaaataa taaggataac gataaccaga atctgaatta tcgtatgtgt cattaagata
    27601 cgcgctgata tctgtctggc tttctgcttg ttcttgttct tttgtgattt ttccgttttc
    27661 ttccattaca cttagtactg tgtttttccg atcattcgca ttttttgggt gatcgatcgg
    27721 attataaata ccaggacctt tcagcattcc tgccaaagta gcagcctcag acagcgtaac
    27781 attcgttgca tctactccaa aatactttct ggaagcgtct tggacacccc ataccccgtt
    27841 gccaaaatag gcattgttaa gatacatggt caagatttct tctttgctgt acttcttttc
    27901 gatctcgatt gccaaaaata attccttcgc ttttcgatca aacgtttggt ctaatgtcag
    27961 atacgcattt ttggcaagct gctgagtgat cgtacttcct ccgccccctg atgttccgcc
    28021 agacaagacc attcggactg ctgcacgggc aatccctttg atatcaaacc cgtgatgctc
    28081 gtaaaaattc ctatcttctg tcgagattac tgcatcttgg atatagggag aaatcgcgtt
    28141 taattctaca tatgttcctt tttgggcgta cagtgtgcca gcttcttcgt ttttcttatc
    28201 ataaatgact gtcgattggc ttaatcctga ttttaacgtt tcgacatttg cttgttttgc
    28261 taaaataaag aggtagatac ttgtcactaa gacacagatc aaaccaatca gcaaaatcaa
    28321 tttgttcaca tgatattttt tccagatgcg ctttcgccat cgatggaatt gtccgagata
    28381 tggtctgatc caacgccaaa aactgatcaa agcagctttt atcttttcta aaatgctttg
    28441 aaaatccatt tgcttcttct cctgtctatt tgattcaatc gtcttatttt accatagtga
    28501 aagagtgttt tcttcctgct taagttgtag ttcaatatgt ttttttacgt tagtgcaagt
    28561 agagtaacat aagaatgaac agaaaaatgt aagttttttc ttaaatatgt tttaaaaatg
    28621 gtacaattag tctgcgattg cactgaaagg agtacacttg tgaaaatcac gatcacttta
    28681 cctatgaacc atcctgaaac aacaattcgc gacttgcttg aaaaggaatg gttagttcct
    28741 agaaaggttc gacacttttt gcgtatacgt aaaaacgttt ggatcaatca acaacctgct
    28801 ttgttccatc atgaagtaaa gggcaatgac cagatcacat tgttattcga agaaacagat
    28861 tataattatc aagaagttca attaggagat gcgaagcaca tccgtgtttt atatgaagat
    28921 gagcatttga ttatagtgga taagcctgca ggaatgaaga ctcatccaaa tgaaccgaca
    28981 gaaaacagta ccttactcaa tcatttagct gcttatctcg acactaaaca gcaacgacct
    29041 tacgttgtcc accgcttgga caaagaaaca agcggtgccg ttttatttgc aaaaaatcca
    29101 tttgtcttgc cgatacttgg aagaatgttg gaacaaaaaa tgatctatcg gagatatcaa
    29161 gccatcgtat ggggagaaat tgccaaagat atgaccatca ccaataaaat tggacgagat
    29221 cgccatgatc ggagaaaaag ggtcgtagat gataaaaatg gtcagagcgc cttgacacat
    29281 gtcactgtca acaaagtact ttctgaacaa acacaagtct attgtgtttt ggaaaccgga
    29341 cgtacacacc agatcagagt tcatttagct cataagggac atccgatcgt tggagaccct
    29401 ctctaccaaa atagaacagc tgaacgactg atgctccatg cattagaact gcacatgatc
    29461 catccattta ctcaagaaaa aatagtcggt aaagctttac ctggattatg ggaatagaaa
    29521 taaaaaagaa cagagtctta tcagcagata atgataagac tctgttcttt ttgtgtaatt
    29581 tgtctgctta cttttgatgt tcgtgttctg ctaacagcaa actaccgata atcccacttt
    29641 cattcggcag tccccaacga acgatatatt cacttacttg aggtgtttcc atataaccac
    29701 ccatcaattc aacaaattgc tgacggattt tttgaaggag atgatcctga ttcattaccc
    29761 caccgcctaa aataatcttt tcaggtgcta acgtcaacgt atagttgatc agtgcttgag
    29821 caatataata agcctgaata gcccaaacag gatgatcttg cggcagatct tgccctttga
    29881 tccctgtgcg tgcctcaata gacggtcctg ctgctaatcc ttctaaacaa tctttatgat
    29941 aaggacaagt accttcataa tggtcatctg gatggcgttt cacccaaata tgtcccattt
    30001 caggatgtgc gatccctgaa aaaatttctt cattgatcac gacacctgca ccgattcctg
    30061 ttcccacggt aaggtaaata caactatttt ttccttgtgc tgctccttgc ttcaattccc
    30121 catatgcagc agcattgaca tccgttgtcc aagcagcagg aatattgtag cgttccttga
    30181 ttgtgccaag aaaatcgaat tgtgcccacc cttttttggg agttgccaaa acgtatccgt
    30241 aattatcttt tgttttgtcg ataccaatcg ggccgaatga gccaatcccg attcccttca
    30301 attcataacg atcaaaaaat tcaaatacct gatccaatgt ttcttctggt gttgtggtag
    30361 ggatacttaa tttttctaaa atttctccac catctgaaac cgcacacaca aattttgttc
    30421 cgcctgcttc tattccgcca tacattcgtt ttcctcctcg aatccgattg tccgaatctc
    30481 ataaggttgg attattggga caaccgcttg cttcgttggt tcttcaagca gattcaatat
    30541 gacagttttt ccattgtctt tagttatttc caatttttct aaatgactag acatattgaa
    30601 acctcttact actactttat cactaaactt gctgcgtttc aaagctgtaa ttgcaaacgt
    30661 ttcacttttt atcgttaaat attgttgttt tgaaatcaat gttccttcat gatgtttgat
    30721 ttgttgagtg gaaaaaggaa tctgtgctgc gtatgcatgc aaataagtag agaatttttc
    30781 ttctggtcca tgtagttcaa tgctgtaatt gaaccgatgc tcacctagac actgtgcttc
    30841 tggtgttgga aaatatcccc aatcgcctag ttctcccaca ctgcgaagta gcgtcactgc
    30901 gatttgtccg tcttctgtta cttcatattc attcaaacca aagtttccga ctgttacacc
    30961 gtactcttca tcatgaatat tgacaaatgc ttgctgatgt tgcggattcg tggggttttc
    31021 ccaactttta gacacatggt ttgggcgctt caccacttca aaaatactat ctgcttcgtg
    31081 atgttccaca tgtaactttg taggaaacaa gacacgtaag cgatgatctt tcatctgatt
    31141 gtcgagagtc gtttcaaagt cgattttttt actatctttt ctgattgtca tgattgtttt
    31201 tatttgaagg attcgttttt ctttggatcg ttctgctttt cgataacgaa attccacaac
    31261 catctgctgt tctttttcta aacgctcgtc tgcagaaaca gggatttcca tttcctgtag
    31321 aatacttatt tttgctattt ccggtgtatc tactaatact tttacttcag agttttcttt
    31381 gttactggat aggattggct gatcacagaa aggttgttta aagatatact catttcctat
    31441 atcgccgata tcttcaaaaa tatgcagatt attcaacttt cgtttgtttt tgcgatcttc
    31501 cattgtaatg gtaccatttt tttcaatcgt caaatgtaga gggccgtttt cgatgattcg
    31561 tccactctga tgaatcattg tttccttttc ttctgctttc gcttcccctt ctacaagcgc
    31621 gaatgtttcc caagaaaatg ctggcatgtt ttctaatggt aaagtcactt tgatcattct
    31681 tgccatatat ggcacacgga aacggtcttt cggcaaatca taaccaaatt ggacagtttc
    31741 ttcagataaa atagctggta tagtggcccc actttttgtt tctacatgat acttacgttt
    31801 aggcaaattt tctaactctt gatatagttg ttcaggtatt ccttcagcga atctttttct
    31861 ttctaattcg atggttattt ctgcttcacc agttttatcc attcctgcag tatttacaat
    31921 gacaaatgga aagctgtctt ttgggaatcc ggtcgtatcg attgctgcgg tcagctgttc
    31981 taaggaatct tgtgcaagat actttccaac ttcatctgct ttttcaaaac gaggtatcat
    32041 ttcccgatgg acagaatcga ctgaacaacc gcaaatactg tcgtgaggat gattttgcat
    32101 taatgtcttc caagcataat ctaattgatc atggggatag tttccactaa cttcataagc
    32161 catcgtcgca agtggttctg taatattttc tagctgtcgc tggaccttgg tattccattg
    32221 ttttaagtaa acccgagcag atgctgtatt ggctaaagta taccagccat ccgtttcttg
    32281 acttgtcaat tctccttcta ctgagcctaa gtcttctggc acatcttttt gaactgcttc
    32341 cagatagtca gtaaaattag aatgaataaa ctcgtaatct ggaaacagtt catttgcgag
    32401 atggattgct tttgatatat ctttttgtac cggttgatga tcaacaccat tcatcatcaa
    32461 taagtggttc gtagaggcat attgttctgc atccgctaat ttttgcttcc aaaaggcaag
    32521 agctgcttct ttttctactg gaatctcatt cccgttgcta taccaattgg caaatagcag
    32581 accaaagatt gctgtttgat cagggccttt ccaccacatc tctgagtatt gggaagaata
    32641 gttttctgct tccaaaactt gattatcaaa gccaattggt tttacaccac ggccaaacgc
    32701 tgctgctgaa ataccagctt gtttcattaa ttgaggcgtc tgtcccatat ttccaaatgt
    32761 atcgggaaaa tagcctaaca tgacaggggt tccccattta cggctttctt ccatgccaat
    32821 cagcatattt ctcacgttcg attccgagct gataagaaaa tcatcttgca agatataaaa
    32881 tggtccaatt ctcaattttc cttcattgat tgcttgttgg attgcctgtc ttttttctgg
    32941 ccttacttga agatagtcat ctaaaataat cgtttgccca tccaaatgaa aactgttgaa
    33001 ggacggatca acttcaaata gctctagtag atcgtctatc aattcaatca gtcgcatgtg
    33061 atgctgttcg taagccatgt accattcacg atcccagtgg ctatgagaaa tgatatatac
    33121 tttctttttc atttacgata cctcctactt ttctactcta atatcaaagt aatccatgac
    33181 taattcgcaa aacatcatat ttgcccaaga aaaccattct ctcgtataat tttcaggatt
    33241 gttcacatcg aatccttcgt gcataaggtg tgttcctgcg tcagttgcta caagtaaatt
    33301 caatatccgc tctttttcag atttgtctga cgttgtcatt ccttccatag ctaatgcaat
    33361 cggccaaacg tatttttctg gcgtatggga ggatccaatt ccttttgcgt actttccttc
    33421 atagaaatat ggattttctt tactcaacaa tgttcttctt gtttgcagat aatcttcctc
    33481 ttgttcagaa caatatccca agtaaggtgc ggcgatcaga ttgggcacat tactgtcatc
    33541 catgactgtg gcgtttccca ttccatctac ttcatatgca taaatcgttt gccctttttg
    33601 attaaccgtt cgtccgtgat tctgaatccc ctcttggatc tctttcttca atttctgtgt
    33661 ttgctgtaca atttctttat catcgagtat cgtttgaaaa atttcttcca cataacctaa
    33721 cacgaccacc gcaaacatat tggatggtac cagatatcca taacggcaag catcatcgct
    33781 aggacggaaa ccagaccatg tcattcctgt aaaagctgtt tttgcacctc gcccgtcatt
    33841 gatcagcgta tcttcttttc ttgtcgtgtc tcgtacaaat gtataaggag agtgttcatg
    33901 atcttgctct gttttgaaca cgtccaagat ttttttgata ccttcaataa aagattgatt
    33961 gaattgatca gtttttcctg tatttttgta aagaagatag gccaactgta ttgggtagca
    34021 taaagaatca atttcatatt ttctttccca gatccaatcg ttcatttttg tatgatctgt
    34081 ttggtggcca gcaccgttag cttcttcgtt gaaggcattc gcatatggat cgatattgat
    34141 ataaaaaaat tgctttttga ctaacccgct gatcatgtca gctaattcct catcttcttt
    34201 tgcaatcact aaataaggac gaacttgtgc agtagagtct cttaaccaca tcgctggaat
    34261 gtctcccgtc aataaaaagg tggttccgtc attttgcttt ttgactgttg tcaacagagt
    34321 attggcaaat gctgctgtaa aattctttgc ccagtccgca tggatatccc cacatttttg
    34381 tgtgatatct tccataaatt tctgcaccga ttttggtata tctttataaa ccactaataa
    34441 aaccttcttt ctatttgata tatctttaaa gattatactt catacagcca attatgtcta
    34501 cgctttcaaa aaatatcttt ttatttactt tttcatccat tatggtatta tttgaatatg
    34561 atatatcatt aagaggtgtt tatatgactc aaccattgta ccagacaata tttaatgatt
    34621 tgcaaacggc tattttaact ggtgaattac ctgtagacag ccaactacca actgaaaaag
    34681 aactttcacg tacctataaa gtaagcagaa tcacttccaa acgtgcattg actgaattag
    34741 agcaattagg acttatttat cgcgttcgcg ggaaaggcag tttcgttaaa tccgcttctc
    34801 cggaacagat tcttgcttct cctacaaaaa cacgacggat tctgtttctt cttccatatg
    34861 tcgctgattt gtctgttggt gattttagca agggattgaa ccctgtgatg caagataaac
    34921 agattgatgt catgatgacg acgcttgact ttctacaaaa taaaacagca aaagagctaa
    34981 tcaatgaatt tgacggattg atttattatg ctcttgatac agaacaacat cttgacttac
    35041 tttttgagct ttcattgaaa gaattccctg tcgtcatatt agataaaaaa atctatgagt
    35101 taccttttcc taccgttctt tccgataatt tccaaggcgg tgcgcttgct gcctcttatt
    35161 tgatcgaaca gggacattca aaaatcgctt atttgtttgg tacgcagact catccacaat
    35221 ccgttcgaca gcgttacctt ggttaccttg aagcattgga caaagctggt ttgactttcc
    35281 acacttcact ggatgacaaa agcgcgacat tagatgaatt gctttcttat atcactaagt
    35341 atgaagtaac tgcttttgtt tgtgaaaacg atgttgtcgc tatccagacc atgaatctct
    35401 tgagacagca tggttatcat atccctgaag atttttcaat tatcggattt gatgatatcc
    35461 aagcagcagc tcttgttgac ccgccactga caaccatcgc acaagatttt gaacagttag
    35521 gtaagcttgc gggagatgct ttggtcactt ggttagagac aaaagatcga ccatccgata
    35581 gtaaatttcc agtttcattc atcaaaagac aatcaacaaa ggagaaataa aatgaatatc
    35641 ggtcaaatag atactagaca tgcaacagcg aatcagccaa atttctctaa tgggaactgc
    35701 cagccttata ctggcgttcc ttttgggatg aattattttg caccgcaaac tacggatcaa
    35761 aacggcagct ggtggtttca tcctgatgac cgcgtttttc aaggctaccg tcttacccat
    35821 caaccaagtc cttggatggg tgatttcagt catatgctgt ttacgccgat caatggaaaa
    35881 ctacaggaga acaccctttt ccatgctcaa agttcttacc gcccagaaga aagtatattt
    35941 tgcccgactc atctgtcgat ctgtcaatta cgttatggca tctgttctac attgatacct
    36001 agcatgtatg gcggaatctt atctattgat tacagcaaaa atgacagcgg tttactttta
    36061 tcctttccgg gacgccatca gctctttgtt atagatcctt acacagtgga aggaaaaatc
    36121 acacattttg ctggttcaga agatcctgat ttttcttttt attttatatt gagatttgaa
    36181 tatcctttaa caattgatga gttagctgtt tccaacaaaa atagcgactc gatcccgttg
    36241 tattttgaac agacaaaaaa acaaactatc cgatttggga catcttttat cagctcggaa
    36301 caagcagctt ataacctgga actagaacaa aaccaaagca aagagactta ccttgctcac
    36361 agtcaagaac aatggcagtc ctattttgat cgtatacagc tcacacacca tgatcaacaa
    36421 caattgcgga cgttttatca taatttatat cgggtgtttt tatttccaca aacattttat
    36481 gaaattgatc ctagtgggca aaaaatccac tatgatacga ctagtcgatc tataaaacct
    36541 ggtgttctct atacaaataa tgggttttgg gatacgtata aaacggttta tcctctttat
    36601 tctttgattg ctgtagaaaa atacgaagaa atgctggaag gttttttgaa cagctataga
    36661 gaaagtggct atttgccaaa atggctgtct cctgatgaac gtggacttat gcctgggaca
    36721 ttgatcgatg cggtcattgc agatgctgct gtaaaagata tccgacgaga cttgatgcct
    36781 gaatttctaa cggcaatgga aaaaggtgct acggttcaaa gcaaacatcc aaattatgga
    36841 cgccaaggaa cagaagatta cttgacatat ggctatgttc ctaatcacta ccacgaatca
    36901 gtcaatcaca cattagacta tgcttatagc gatttttgta ttagccaagt tgccaaggta
    36961 ctagaagaag aggaaatgac gaagaaatat cagcagcaat cattgaacta tcaaaatatc
    37021 tttgactatg agtcaggatt tatgcgttca aaagataaag atggcaagtt cagagaacca
    37081 ttttcagcta atcgttgggg acaagattat gcagaaggaa gtgcttggca aagcagcttt
    37141 gctgtttatc acgatttttc aggactgatc ggaaaatatg gtggcagtga gagatataaa
    37201 gaaaaactca ttgagctgtg caatcagccc ccaacgtttg acccaggagg atatggtttt
    37261 gagatccacg aaatgagtga gatggccgcg atagaatttg gacaattagc tatttcaaac
    37321 cagccaagtt ttcactatcc ttacttgttc agttatatag gacaacctga aatggcacag
    37381 ccactgctta aacagttgat gactcagacc ttcgacgatt ctccaatggg ttaccctgga
    37441 gatgaggaca atggaagtat ggccgcttgg tatatcttca atagtctggg attttatcct
    37501 gtcacgcctg gaaccggtga atatgttatc ggtatgccat taattgacaa ggctgttttg
    37561 cttttgtcaa atggaaagga attgacgatt gaagcaacgc ctaatcggcc gcagcaacaa
    37621 tttatccata aaatcaaacg gatggacaga gaatatacga aattatattt tacccatcaa
    37681 gatttgctga aaggcggcac gatctcttac caattaggga tcatccctca ccctaagaaa
    37741 tatcaagcaa aagatttgcc tttttcctta tcaaactata attaacaaga ggttgtgaaa
    37801 aagctgtcct gcatcaagta ataagaacag ccaagcaaaa atagatcctc atatttttcg
    37861 tttggttggt cttattaccg cagatgcaag cttttgaaca ccgtttattg agaggttgtg
    37921 aaatcagcgt tctgacctga gtattagaga gacaaatggc aaaatagttc ctcatatttt
    37981 gccatttttt gagctaatac cgagggtcag ctgattgaac accatttagt ggagaggtcg
    38041 tgaaaaagct gtttagtttc gagtattaag aaaaattttg tgaaaatggc tttccacatt
//
LOCUS       DAYYCW010000013        35916 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000013 DAYYCW010000000
VERSION     DAYYCW010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.013"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            174..1139
                     /locus_tag="ACHAYA_000661"
     CDS             174..1139
                     /locus_tag="ACHAYA_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8604490.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(1196..1426)
                     /locus_tag="ACHAYA_000662"
     CDS             complement(1196..1426)
                     /locus_tag="ACHAYA_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM8604491.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1512..2399)
                     /locus_tag="ACHAYA_000663"
     CDS             complement(1512..2399)
                     /locus_tag="ACHAYA_000663"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8604492.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2488..3711)
                     /locus_tag="ACHAYA_000664"
     CDS             complement(2488..3711)
                     /locus_tag="ACHAYA_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8604493.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3728..4774)
                     /locus_tag="ACHAYA_000665"
     CDS             complement(3728..4774)
                     /locus_tag="ACHAYA_000665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8604494.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(4938..6665)
                     /gene="ptsP"
                     /locus_tag="ACHAYA_000666"
     CDS             complement(4938..6665)
                     /gene="ptsP"
                     /locus_tag="ACHAYA_000666"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM8604495.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(6665..6931)
                     /locus_tag="ACHAYA_000667"
     CDS             complement(6665..6931)
                     /locus_tag="ACHAYA_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM8604496.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            7410..9644
                     /locus_tag="ACHAYA_000668"
     CDS             7410..9644
                     /locus_tag="ACHAYA_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8604497.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9792..10112
                     /locus_tag="ACHAYA_000669"
     CDS             9792..10112
                     /locus_tag="ACHAYA_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8604498.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(10317..10541)
                     /locus_tag="ACHAYA_000670"
     CDS             complement(10317..10541)
                     /locus_tag="ACHAYA_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFM8604499.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(10739..12319)
                     /locus_tag="ACHAYA_000671"
     CDS             complement(10739..12319)
                     /locus_tag="ACHAYA_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM8604500.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12720..14096)
                     /locus_tag="ACHAYA_000672"
     CDS             complement(12720..14096)
                     /locus_tag="ACHAYA_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8604501.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(14224..15312)
                     /locus_tag="ACHAYA_000673"
     CDS             complement(14224..15312)
                     /locus_tag="ACHAYA_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8604502.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15484..15918
                     /locus_tag="ACHAYA_000674"
     CDS             15484..15918
                     /locus_tag="ACHAYA_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8604503.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(15996..16529)
                     /locus_tag="ACHAYA_000675"
     CDS             complement(15996..16529)
                     /locus_tag="ACHAYA_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM8604504.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16623..17801)
                     /gene="mutY"
                     /locus_tag="ACHAYA_000676"
     CDS             complement(16623..17801)
                     /gene="mutY"
                     /locus_tag="ACHAYA_000676"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM8604505.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17794..18591)
                     /gene="recX"
                     /locus_tag="ACHAYA_000677"
     CDS             complement(17794..18591)
                     /gene="recX"
                     /locus_tag="ACHAYA_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM8604506.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18698..20080
                     /gene="rlmD"
                     /locus_tag="ACHAYA_000678"
     CDS             18698..20080
                     /gene="rlmD"
                     /locus_tag="ACHAYA_000678"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8604507.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(20254..20832)
                     /locus_tag="ACHAYA_000679"
     CDS             complement(20254..20832)
                     /locus_tag="ACHAYA_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM8604508.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            21154..21624
                     /locus_tag="ACHAYA_000680"
     CDS             21154..21624
                     /locus_tag="ACHAYA_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM8604509.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(21732..22958)
                     /locus_tag="ACHAYA_000681"
     CDS             complement(21732..22958)
                     /locus_tag="ACHAYA_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8604510.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(23712..23912)
                     /locus_tag="ACHAYA_000682"
     CDS             complement(23712..23912)
                     /locus_tag="ACHAYA_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8604511.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(24046..24654)
                     /locus_tag="ACHAYA_000683"
     CDS             complement(24046..24654)
                     /locus_tag="ACHAYA_000683"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM8604512.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(24739..25209)
                     /locus_tag="ACHAYA_000684"
     CDS             complement(24739..25209)
                     /locus_tag="ACHAYA_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM8604513.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(25257..26171)
                     /locus_tag="ACHAYA_000685"
     CDS             complement(25257..26171)
                     /locus_tag="ACHAYA_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8604514.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            26317..27120
                     /locus_tag="ACHAYA_000686"
     CDS             26317..27120
                     /locus_tag="ACHAYA_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM8604515.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            27298..28245
                     /locus_tag="ACHAYA_000687"
     CDS             27298..28245
                     /locus_tag="ACHAYA_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM8604516.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(28313..28606)
                     /locus_tag="ACHAYA_000688"
     CDS             complement(28313..28606)
                     /locus_tag="ACHAYA_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM8604517.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(28634..29299)
                     /locus_tag="ACHAYA_000689"
     CDS             complement(28634..29299)
                     /locus_tag="ACHAYA_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM8604518.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(29439..30071)
                     /locus_tag="ACHAYA_000690"
     CDS             complement(29439..30071)
                     /locus_tag="ACHAYA_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8604519.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(30078..31145)
                     /gene="liaS"
                     /locus_tag="ACHAYA_000691"
     CDS             complement(30078..31145)
                     /gene="liaS"
                     /locus_tag="ACHAYA_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM8604520.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(31142..31873)
                     /gene="liaF"
                     /locus_tag="ACHAYA_000692"
     CDS             complement(31142..31873)
                     /gene="liaF"
                     /locus_tag="ACHAYA_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM8604521.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(32042..32521)
                     /gene="greA"
                     /locus_tag="ACHAYA_000693"
     CDS             complement(32042..32521)
                     /gene="greA"
                     /locus_tag="ACHAYA_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM8604522.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(32657..33832)
                     /gene="mltG"
                     /locus_tag="ACHAYA_000694"
     CDS             complement(32657..33832)
                     /gene="mltG"
                     /locus_tag="ACHAYA_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM8604523.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            34036..35205
                     /locus_tag="ACHAYA_000695"
     CDS             34036..35205
                     /locus_tag="ACHAYA_000695"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8604524.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(35472..>35916)
                     /locus_tag="ACHAYA_000696"
     CDS             complement(35472..>35916)
                     /locus_tag="ACHAYA_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604525.1"
                     /translation="FQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQ
                     VIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKS
                     ESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT        10843 a         7210 c         5917 g        11946 t
ORIGIN      
        1 aaaacaactg ataaatcagc atttataatt tcttttatta gaaaaatcat ctatctcaca
       61 tattttgatt atcaaagtat tttctgtgaa cagaactata gaattcccta aaacttgtcc
      121 attatttcaa aatttgttat agtcttttta ttcaaagcgt aaaaggagat cctatgttaa
      181 aagaaaaaat gaaaactctc cccatgctgc cgggtgtcta tcttatgaaa gaccgagatg
      241 gtaaggtcat ctatgttggt aaagcaaaac ggctcaaaaa tagagtaagc agctattttc
      301 atcagaataa acagcattca aaaaaagtat tacgtatgat tcatcatata acagattttg
      361 atttcgtcgt agtagacacg gaattagatg ctttattgct cgagtgtcaa ttgatccaac
      421 attaccgtcc tttttataac cgacaaatga attatttcag taattataat tatgtacata
      481 ttaccaataa gggatttgta ttaacagata ctcctacggc tcgaacatac gggccttttc
      541 gtctctataa aaaaatgccg tctattttgc gtataatgga agaaacttat caaatgcctt
      601 ggttgtctga aattagtttg ttagctttgc gagtccagct tcctgaccta caggaaatga
      661 cttttgaaca gaaaaaaaag gaactccaag ggctgtttca aggccgaaat aaaaagctac
      721 tgacttactt gaaaaaaaga caacaacatt tcatctatca gttgaatttc gaaaaagccg
      781 gtatgcttca aaaagatatc gaattagtta cttattttat tagacggatc caggaacaaa
      841 agcagttttt aagaacacca tcgctcacct tcagcatgcc attagcagca gatgagagtc
      901 agaaaaagca ctacctcgtt tgttatggtc aacttgcaga aacaatgatt acctctggtg
      961 acgtttctcc tgacttctat tatgaaaaga aagaagctca tttatctttg aaacgtcaat
     1021 tgtcaaaaga agaaatcgat cctgtacaaa tcttgatcag ttaccgcaaa aaattagaaa
     1081 aagaacagtt tgaaattgaa ctgttaaaca aaaaagaagc tgaaaagcaa ctaaattagt
     1141 tgcttctcgg cttcttttag gcatcttcaa cttcaaatgt caattatcct aaaatttatt
     1201 ggattaagtc atagatttcc atcgctacga tatcgatatt atcaaattcg tattgctgat
     1261 gagtatctgt ttcagttaat tcaaatgtat cggtcgcttt atcataagtt actgtacact
     1321 tctctacgcc ttctttttca aaacggcgaa cttgcagttc attatctgct gactcgacca
     1381 tagcctccag tcgttttatg attgcgacta attgtgaatg tgtcatcgga ggtcctcctt
     1441 ttcttatttc tgttatattg tatcaaaagc atagcctact tgcattgatt ttctctaaaa
     1501 aaactcgttt tttatgattt tttttctggt atttttcctt caggccacca aagtttgatc
     1561 aaagcttggg ttccgtcatt ctcaaaacct ttatctgcca actcttcata tagtttcaat
     1621 gcttgggtag ttgctggcaa tacaagatcc attttttctg cttcttctaa tgcaatcttt
     1681 aaatctttga taaaatgttt gacaaaaaaa ccaggtgtgt aatcttcttt taaaatacga
     1741 ggagaataat tgcttaaaga ccagttagct gcacttccac ctgaaagcgt ctcaatcact
     1801 ttctttaaat caagtccgtt tttttgtgca tagacaagca tttccgtcat tcctgtcatc
     1861 gtacctgcaa tcatgatctg attagccatt tttgtatgct gacctttccc agcagaacca
     1921 tgcaatgtaa atgtcgtacc aaattctttg aataaaggca gtacttgatc ataaacggct
     1981 tcttctccac cgaccatgat cgtcagtgta ccattctttg ctcctaagtc accaccggat
     2041 accggtgcat ctaacgcatc tgcgcctttt tcttttgctg tttttgcaat tttttctgcc
     2101 aggcttggtg tacttgtagt caaatcaact aaaatatgtc ctgagatatc tgcagaaaaa
     2161 atccccgact gtccatagta gatttcttct acatctttgg ggtaacctac cattgtaaag
     2221 agaatattac tttgttctgc taccgctttt ggtgtatctg cccagatagc tccttctgtt
     2281 actaaaggat cagcttttgc ttttgtccga ttatagacag ctacttcgtg tcctgcttct
     2341 agtagatggc gtgcgacagc acttcccatg acgcctgttc cgataaaacc aagtttcatt
     2401 ttttcgacct cctaaaaatc aaaaatcctt gcttctatgc tagtatacgc tagaaacaag
     2461 gattttttca taaaaaatgt ttgaatttta tcttttaaat gaatttaatt ttattttgat
     2521 tcgttcaatc gagtcttctt cttcttttct tcgcatctct tcttcatgat aaacaaccat
     2581 gtcttgataa aattcgatca tagcttcgcc gaaatgtgta gatgaaatat cataaagttt
     2641 agcttctaaa acttttggat ctttaggaac ttcactggaa atataatcaa taagtgctgg
     2701 tgcaaagtct tcgtcggttt caaaagtttt ccctaatgaa ggatgatcga acaatcggtt
     2761 caaataagca tttccttcca cgacacaagg tgttccggct gccatcgctt ctgtataagt
     2821 aagcccttgt gtttctgaag tagaagcact gacaaaataa tctgccgctt tataataaag
     2881 tgctacatca ttgttgtcca cttctcctgt gaactgcaca aattcttcta actgcagttc
     2941 tgagatcaat tcctctaact cttctacata aggtcctttt cctacgataa ctaatctggc
     3001 gttaggataa gcggagatga cctgaggaaa cccattaatc aaggcttgga tatttttctc
     3061 ataagaaatc ctgcttaaag aaagaatcat caaatggttt tcctgaagac ctagtgactc
     3121 ccttaatttg ctgatatcct gctgagtgat atcagaacgt ttgaattttt caatgtcgat
     3181 accagtagga atcacgcgaa gcggtgctat tacaccatag ctgcgtaatg tatcaataac
     3241 acgttcactt ggacaaacta cacctgtcgt atgatttgca aataatctag acaagtattt
     3301 tacatgggat tgcctgatca cttttccttt accgatataa tgaaggtagt cttcatacat
     3361 cgtatggtaa gtatggatca gaggtatttt caacttctta gctaccatct tcccaagtat
     3421 ccctgctccg aattctgtat gggtatgaat aagatctagt tctagttctt tggcaaccaa
     3481 ataagcatac cacatgcctc taacaacaac tcttcgatcc ttgaaagaaa taaatggtac
     3541 acttggcatt cgaatcacat ctttttccaa cttatccgca ttaggatcgg tcgttgtaaa
     3601 aatatagact tcatgcccat gtctttctaa ttcttgtttt aatgttttga tcgatgtagc
     3661 aactccgctg acttgcggaa agtaggtatc ggtaaaaaag ccgattttca tgtttttcgc
     3721 ctccaaacta gtccttgacc tgatttactt tttttaattt gctagagact ttttcattca
     3781 gtacactttc gtatacattt ttaagttctt caccgatttt attgatactt cgctcttttg
     3841 ctgtttgata accggcttgt gtcgtactcg gtagttcttg attggtaatc tgttggatca
     3901 gtcggataaa ttcttcgttt gtcttcccca tatagcagtt cttgctatct tctaaccagc
     3961 cattatacac tggaatatct cgaacaagta cgttttgttt gcttgctaaa gcttctaaaa
     4021 caacgatccc ttctgtctct tcataagaag ggaaaaagaa tagatctgca cctgaatacg
     4081 caccttctat gatctctcca cgaatgtaac caggaaacag gacattttca ggatgatctt
     4141 cttttacaat tttccggatg ttccgaggaa tcgagtacat gggaacatga ccaaaccaaa
     4201 taaatgtgta ttcgggcatc ttcttagcaa tttctacgaa atcaatgatt cctttacgtt
     4261 cgaaaaacaa accgacacaa atgattactt tttgatccgg agacagtttg aaatattctc
     4321 tgaacttttg ttctttcatt ggatcagggt gatatttctc taagtcgatc ccattggaaa
     4381 tagcttgaat cggcacacgg ataccatagc tttccaacaa ctgttttgaa taaggtgttg
     4441 gcgtgatcag atagtccgct ttttggtaca atcctaccag atattttttt acgataggag
     4501 aaagctggtt cgatctgata aaggaatttc ggaaatcttc ctctgtagaa tgcgcatgat
     4561 agacaacttt ttttccttct cttctggctt tattgaccat attatgacta ttgattccgt
     4621 atgtattgat gtgcaggatg tcataatctt ctttttcatc tagtgtataa gagataccga
     4681 cttccgtcag agcacgtttt tgatgatcaa gcgcacgccc gattccagat tttgcaagca
     4741 tcttttcact ttcaaaatat aataatacct tcaccttacg tcccccttca cttatatagt
     4801 ataacatagc ctacaaactg tcactcaaac ttaagcccta tttttgtcta ttcttactga
     4861 caaaaacgtt ttcttaaaaa agaacaaaaa aactggcgta gctcttttac aagccacacc
     4921 agcttttgat acgttgttta ttttacatat tcatgaacaa gttcaacgac ttcttccatc
     4981 atatcgcatt ctttcaatgc acgatcagca agttcagcca ttttcgctgt gtccaagcgt
     5041 ttcattaaac tacgcgtttt aagaatagat gtcgcactca ttgagaactc atctagaccc
     5101 atacctacta gcaatggaac agctgtttga tctccagcca tttctccaca catacctgcc
     5161 cattttcctt cagcgtgtgc tgcatcaatt acatttttga tcaaacgtaa aattgatggg
     5221 ttatatggtt ggtacaagta agaaacgcgt tcgttcatac ggtctgctgc cattgtgtat
     5281 tgaatcaaat cgttcgtacc tacagagaag aaatctactt cttttgcaaa cttgtctgct
     5341 aagactgctg ctgctggaat ctcgatcatg atgccgactt ggatcgtatc agatacttcc
     5401 ttgccttcac ttatcaattt ttgtttttct tcttcaaaga tcgcttttgc tgcgcggaat
     5461 tctttgagtg tagctaccat agggaacatg atacgtaaat taccatgaac agatgcacga
     5521 agcaatgcac gcatttgtgt acggaacatt tcgtcgcctt gttcagataa actgatacgc
     5581 aatgcacgat atcctaagaa tggattcatt tcatgaggta attgcagata tggtagttct
     5641 ttatctccac cgatgtccat tgtacgaaca actacaggtt ttccttccat accttctaat
     5701 acagctttgt aagcttcata ttggtcatct tctgttggga agtctggaga atccatatac
     5761 aggaattcag tacggtacag accaactgct tctccaccat tattatggac accaactaga
     5821 tcttttggtg ttccgatgtt tgcagctaat tcaaaatgct tgccgtcagc tgtgacagtt
     5881 tctgcatttt ttaatttttc ccattctgct ttttgtgctg cgtaatctgc acctgctttt
     5941 tcaaattcag ctttttgttc atctgtagga tcgatgatta catcgccttc gatcccgttg
     6001 acagccaaaa tatcgccttc tttgacttta gcagtaattt cttttgtccc aacgattgct
     6061 ggaatttcta aagaacgtgc catgatagca gaatgtgaag tacgtccacc aatgtctgtt
     6121 acaaacgctt ttacaaaatt gcgatctaat tgtgctgtat cacttggagt caagtcatgt
     6181 gcaacaacga ccacttcttc gttgatcatt gatggatttg gtaaagtaac gcctaataag
     6241 tgcgctaaaa tacgtttagc aacgtcacga atatctgcag cacgctcttg catataagca
     6301 ttatcttcca ttgcttcgaa cataccgata tacatatccg ttacttcttt tagcgctgat
     6361 tcagcattta cgctgttgtc tttaatattt tgcttgattt gacctaccat ttctggatct
     6421 gaaagaacca tcaaatgggc atcaaatact tgcgcttctg cttcacctaa gctttgcgct
     6481 gctttttcac gaatttgctg caattcttca gtagatttag ctaatgcagc atccaaacga
     6541 gcttcttcag ccgcagtatc ttctactgtt gccttactga atgttaaatc cggttgaact
     6601 agcaggtaag cttttgcaac ggctacaccg tcactagcgg caatcccttt tagcatttca
     6661 accattattc agataagcct tctttcttca ttgtttcaac gattgctgcc attgcatcag
     6721 cttcgtcagc accttctgca gtaatagtaa catcagaacc ttgacctaca cctaaagaca
     6781 taacgcccat gattgatttc aagtttactg atttaccttt atactctaag tttacatcag
     6841 agttaaattt gcttgctgtt tgtactaata gtgtagctgg acgagcgtgg atcccagttt
     6901 ctgctactac gtgaaattct ttcttttcca tattaatcgg tctcctttaa gtgagtgttg
     6961 tttttatgtg agggttttct tttttagtaa ttcagagaat cctaacaaat gccacccttt
     7021 catctgtaaa gattaacatt ttttttcgtt agatacaaca gttttcattc aatttcagat
     7081 aaaagtattc aaattattcc ggattctttt tcatcctttt cttcacattt tataacaaac
     7141 gtgtttattt cctttttctt gtcgctttct cttctcctga cgtttatcat tctttccttt
     7201 gaccctcttt gttttcatat gaaacgaatt acttgcttcc ctttttattt ttagtataat
     7261 aagagtcaaa ggtcaggaat ggtcaataga gatttcacct tttctacctc ataaatctct
     7321 taaaaaacta ggggttattc ttttacctca tgaaaaacga gatgtaaaat atggatgtac
     7381 ccatttttgt gaaaggacgt gaatgattca tgctttgtca aaactgcggc aagaatgagg
     7441 caacgattca cttgtatgcc actgtcaatg gtcagcggac acaactcgat tactgtcaga
     7501 gctgttatca aaaaatcaaa aatcaacaaa atggaggtct gactaatatg gcccaaaatg
     7561 atccatttgg atttggcagt ttggacgatc ttttccgttc catgtctcgc caaatgcaac
     7621 agcaaggaaa ttatgagcaa acaccaccaa cacaatttgg cggaaacaac aacttcaatg
     7681 gtggtcagcc acctcaaggc aatacagaag gtttattggg agaatacgga atcaatatca
     7741 cagaacaagc acgacaaggt gatatcgatc ctgtgatcgg acgcgatgac gaaatcaaac
     7801 gagtcattga aattttaaat cgccgaacca agaacaatcc tgtcttgatt ggagaaccag
     7861 gtgttggtaa aacagcagtt gttgaaggtt tagcacaaaa aatcgtagat ggtgatgtac
     7921 cgcaaaaatt aatggacaaa gaagtcattc gtttagatgt cgtatcttta gtccaaggta
     7981 ctggtatacg aggacaattt gaagaacgta tgcaaaaatt gattgaagag atccgtcaag
     8041 cagaaaatgt gatcctattc atagatgaag ttcacgaaat cgtcggtgct ggttctgctg
     8101 gtgatggaaa tatggatgct ggtaatattt taaaaccagc tttagctcga ggagaattgc
     8161 aaatggttgg tgctactacc ttaaatgagt accgcatcat cgaaaaagac gctgctttag
     8221 aacgtcgtat gcaacctgta cgggtagatg aaccaactgt cgaagaaacg atcagtattt
     8281 tgaaaggttt gcaaaaacgt tatgaagact atcatcatgt aaaatatact gatgaggcca
     8341 tcgaagcagc cgcaacttta tccaatcgtt acgtccaaga tcgcttcttg ccggataaag
     8401 ccatcgattt gctagatgaa acaggttcta agaaaaactt gacgatccaa atcgttgatc
     8461 ctaaaacaat cgagaaaaaa ctgcaagaag cagaagaaca aaaagtttta gcttctcgag
     8521 aagaagattt cgaaaaagca gcttattatc gcgatcagat caataaactt caaaaaatga
     8581 aagaacgtca gctcactgaa gaggaaacac ctgtgattac cgaaaaagat atggaaaaaa
     8641 tcgttgaaca acggacaggt attccagtgg gtgaattaaa agaaaaagaa cagacacaac
     8701 tgaaaaactt agcagatgac ttgaaagctc atgtcatcgg acaagataac gctgtagatc
     8761 gagtagcgaa agcgattcgc cgaaaccgtg ttggattaaa caaacagaat cgtccaatcg
     8821 gctccttctt atttgttgga ccaactggtg taggtaaaac agagcttgcg aaacaacttg
     8881 cctacgaact attcggttca caagattcaa tgattcgttt cgatatgtct gaatatatgg
     8941 aaaaacatag tgtctctaaa ttgattggtt ctcctccagg ttatgtcgga tacgaagaag
     9001 ctggtcaatt aacagaaaaa gtacggagaa atccttacag tcttgtcttg ttagatgaag
     9061 tagaaaaagc tcatccagat gttttgcata tgttcttgca aatcttagat gatggtcgat
     9121 tgacagatgc acaaggaaga acggtcagct ttaaagatac gatcatcatt atgacaagta
     9181 atgccggaac tggtaaagta gaagcaaacg ttggttttgg tgcggcacgc gaaggtgtta
     9241 ctcgttctgt attgaaccaa ttaaacaact actttacacc agaatttctg aaccgttttg
     9301 acgggatcat cgaattcagt gcattatcaa aagaaaactt gatgacaatc gtcacactaa
     9361 tgctagacga tgtcaaccaa atgctggcag cacagcaact tcacatcgaa gttcctacga
     9421 atgtcaaaga aaaattagtt gacttaggct atgatccatc tatgggtgct cgtcctcttc
     9481 gacgcacgat ccaagaacaa atcgaagatg gtatcgctga attttatctt gatcaccctt
     9541 ctatccatga gttgaaagca aaactggata aagatggaaa aatcatcgtt acttctaaac
     9601 cagaacgttt ggctaaagaa tcagccgaag aaacggctga ataaatccag ttttatagcg
     9661 tgagacggta tttctttgag aaatgccgtc tcattctttt ttcagaaaag tcaaaatact
     9721 gttcatttta taaaaaagaa attataataa gagaaacaag caattaaaaa tacataaaag
     9781 aggtgagcgc catgaaattg gtaaatgtaa caaacagcca ttcccgtctt gtattaaacc
     9841 agcttgaaaa cactgatgcc catctagtca aggtctacac agccggaaat accactattg
     9901 tctacactga agcccctgaa cataatgaaa ttctgctgat caatgacaaa cgtaaaatcc
     9961 agccgaaaga aatcgaagat gctaaaaatt atttcttaga taaaatcaaa gacgccgttt
    10021 accacgagga tgaaatcaaa gtaatcgaat tggcaggcct tgttgaaatc tcgattccaa
    10081 aacatgaatt aggcgctaca ttagccgtgt aaacaaattt tgaacataat aattaaagag
    10141 cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcgaaa
    10201 gcagaagcaa ctccttcgga aataagctga aattcacgaa aattgctttt caaatttttg
    10261 tgaattccag cttatttctt ggagttaaac gcttctgtct cagcctcttt ttctttctag
    10321 ctttttgatt tttctctttt cttttgtact tttctctttt cattttcagt tctacgcgtg
    10381 tctttatttt taactttttc ctctttatgg atgattgatc gtactttttg tgcaatttgc
    10441 tcgtttttgt cccagtcttt tacatctaat aatcgacata atcgtaacat ctcttctttt
    10501 ttctgttttt ttctttcacg tctttgccat tctgtttcca tgtgtcacac tctcctttct
    10561 ttaatcatat gtacgcaaaa taatttttat atttttttga ttagacattt ctatgatttt
    10621 aatatagtaa aaagagccgt cacggaaaat ccgtcacggc tctaaacaaa atatgagagg
    10681 ctgtttttgt ttggctattc ttattacttg atgcagggca gcttgtgtca agacttcttt
    10741 acatcaaact ttttaatttt acattgggat atttatccgc aaaccagcgt tctgcaaatt
    10801 ggttctcgaa taagaacaac ggttggtcga aacggtcacg agccaagata ttgcgacttg
    10861 aactcatctt ctcatccaaa tcttctggat cgatccagcg agcgattttg tgtcccattg
    10921 gagacatgat tacttcggcg ttatactcat ttaacatacg atattggaat acttcgaatt
    10981 gtagctgacc aacagctcca atgatatatt cttccgttag atatgtttta taaagctgaa
    11041 tcgctccttc ttgaactaat tgatagattc ctttgtggaa ggatttttgt ttcatgacat
    11101 tttttgcagt cactttcata aacagctctg gagtaaatga agggagttct tcataagcaa
    11161 cttttagttt tccttcgaac aatgtatccc caatctgata attgcctgta tcatatacac
    11221 cgatgatatc gcctgctact gctttttcga cgttttctct cgaatctgcc ataaattgtg
    11281 tgacattgct taatttcagt tttttatttg tacgttccaa ccagacatcc attcctcgtt
    11341 cgaatgttcc agaacagatt cggacaaaag cgattcggtc acgatgggct ggattcatat
    11401 ttgcttggat cttgaatacg aaacctgaaa attcttcctc ataaggactt accatctgat
    11461 catcttgtgt tttgtgtcca taaggagctg gtgccaattc aacgaaagtc tctaaaaaag
    11521 tctgtacacc gaagttagtc aatgcagaac caaaaaatac tggtgtctga tcgccacgag
    11581 caattttttc ttgattaaat gcatctccag cctctttgat tagttctact tcttctaaga
    11641 cttgttcata taaggaattt tcagttagag gatgattttc gggaagcagt ccatcttcat
    11701 ccaattctac taatcgttca tcctgataat tttccggacg ataaaactct acacgattat
    11761 ggtagatatc ataaagacct tctagccctt tacccatacc gattggccaa ttcatcggat
    11821 aagattcgat atctaatagc tcttctaatt cttctaacaa ttccaatggt tcacgaccat
    11881 cacgatctag tttgttgata aaagtaaata tcgggatgcc acgttttttt acaacttgga
    11941 ataatttctt tgtctgtgct tcgatccctt ttgcactatc gattaccatg accgcactat
    12001 ccacggccat caaagtacga taagtatctt cagaaaaatc ttcatgtcct ggtgtatcca
    12061 agatatttac tcgtttccct tgataatcaa actgcataac agaactagta acagaaatcc
    12121 cacgttgttt ctcgatttcc atccagtctg atttggcaaa attacctgtt tttttgcctt
    12181 ttacggtccc ggcttgacgg atagccccac caaacaatag taattgttct gtaatcgttg
    12241 ttttcccagc atccggatgg gaaatgatcg caaaagttct gcgattgtcc acttgttgct
    12301 ttaattttgg attattcata acctaactcc atttcttcca ttcaattatt tatattttct
    12361 ctaggcacag attgtccgat ctcttaaaga aagacaattt catatagggc tgtgacaaaa
    12421 gccgtgtagc agtctctttt tccgagtaaa cggcgttcta gagacagcct atcagcaata
    12481 agcccaccca agccaaaaat acgaaaagct gtttttgctt ggctgctctt attacttaat
    12541 gcaggatagc tttttcacaa cctttgtcaa ctactacaac tgaatcagta tagcatggat
    12601 tcagcgaatg aaaaagccac caaaccataa gagtcaaata tttttttgtc agctgactaa
    12661 aagaaaagac gtgttgcttg tttattcaag caacacgtct ttcataacca agtttccaat
    12721 tatcttcttg gctcgtcatc ttcgaattct tttctgaagt atcttcgttc ttcttcaggt
    12781 tccgcttctg tttcttccgg atcatgaaaa atgacacgga ttttaaggat tcgagaacct
    12841 tccatttctt cagaaaccag cgtaatatta tcgacatcaa acgataactt ttcgccttca
    12901 tccggaatca tgcctaatgc tgtaatcaaa taaccagcca tcgtatctac atcactcata
    12961 tgcagatcac tgtcaaaagc ttcattgaac tcatcaatca gcatacgtcc ttggatgata
    13021 tattcgtgtt catcgatttt ttcataaaga ttctcaactt catctgattc atcatcgatt
    13081 tctccgacga tttcttctaa aaggtcttct agtgttgcta acccaacgac gccgccatat
    13141 tcatctaata aaatagccat ctgattctgc gtctttttca tttcatataa cagatcatca
    13201 ataaagaccg tttcaggaac gaataaaggc tcctgcatga ttttcttgat atctaaatta
    13261 tcaaagccga atttatgggc agcttttagt aaattctttg tatgaaggat tccgactact
    13321 ttatctttgt cttcgttata aacagggatt cttgaatagt tttcggataa aacttcattc
    13381 acattttctt ccacagtgtc gttgatatca atcataaatg catccgttcg tgggaccatg
    13441 acttctcggg caactttcgt atcaagagaa aaaacccctt ggagcatttc taattcttca
    13501 ttattcaata cgccttcgtt ttcaagcata taacgcattt catctctggt catttttgaa
    13561 tcttcatcat caaaagtcat cggcgtgata cgcgccaata aacttgtgga agcagaaagc
    13621 agccagacga agggcttagc tactacacca atcaagcgta tcagccctga agtgaacttt
    13681 gcgacttctt ctgatttatt taaagcaata cgtttagggt acaattctcc aaaaacaatc
    13741 gatacatagg tcaaaatagc cagcacaatg atatttgcaa tattttttgc cgctgtgccg
    13801 ccacctaata ccggagctaa ccgttccgat aaagtattag ctagtgaagc accagacaaa
    13861 atgttgacca aagtgattcc tacttggatt gtcgatagga aattatttgg attctgcaat
    13921 atttttaaaa gcttttgaga tttcacatct ccttcatcag ctttttgctc gattcgattc
    13981 ttattgatcg aaacaacagc aatttcggaa gctgctaaaa aagcattgat caatgtcaaa
    14041 acgatcaata ataaaatctg cgctaacagc gactgactct cagggtcagc attcataatc
    14101 gcattctctc cttataaaat taatttttag tttacgatag aaaaaaacgt ggcactatag
    14161 ccacgttaaa atggtatcac aaagcaattt tctttgcaat aaaaacaagt gtactaaaac
    14221 tacctacgag gtcgtagcaa ttttcttctt gttttcattt tgcttgtctt ctttgacgat
    14281 tttgattata taggaaatga tcgtcttgca gattgcgtaa aaaggaacac ctaagaagat
    14341 accaagcaat ccggcaatat ttccagctac tagaagaacg ataatgatcg ttaatggatg
    14401 gattttcagt gatttcccaa ttacattagg ataaatgata ttcccatcca actgttggac
    14461 gattagcaca accacacaac aaagtaatgc ttttaccgga tcattgaaga ctgttactag
    14521 aaatgcaggt gctagtccta gataaggtcc taaatatgga attaggtttg tcaatcccgc
    14581 aatgacccca aataagaaag cataatccac accaattacc atatatccta aaaatgtgaa
    14641 ggtaccaacg aataaacatt cgattgcttg tccgctaatg taattagcca atgttttatt
    14701 cagttgcccc aataactcca caatttggtt tcttcttttt tctgggaaaa actgttggat
    14761 gtttggcact agtttttctc cgtctttcaa catatagaac aagatgaatg gtgctgtaat
    14821 gattactacg gttgccgaag cgatcgttcc aacgatcgaa cctaaactat tggatactcc
    14881 actaaggaat tgctgaatga ttgtcccgta agaaatgtct aattgctcaa aatatttatt
    14941 gatatctact tccttgaaca aaggcaattc ggctacctct tttaaccagc tttctacctg
    15001 tttgatgaaa tcaggcatat tagatgccaa tgaggaaatt tgctgtacta gacttgggat
    15061 gacactaagt aaaatccaaa taattgctgc aactaaaagg agcagcacaa ttgcgacagc
    15121 ccatattctt ttgatttttg ctttcatcaa cagcttgaca ataggattca ataaatagta
    15181 aagaaagcct gcaatcagta caggtgcaaa taacgtggaa aaaaatgtcc cgatcggttc
    15241 gaaaataaag tttatttctg atgaaatcat gatcaatgca gctatgatca ataattcaag
    15301 cgaccaaaac attagtttag actgtctaag tttttcaaac aatagatttc ctcctttctt
    15361 tcttactagt atatattata ttcgttaaaa attcttttat tcttttttta tttttgaaaa
    15421 cttgccatac tttttatttg ttttattttc cagtatacta tacgaaagga aggtggctag
    15481 accatgaaag ttgtacaaac aaaagataca atgagcaata tttacttaga tgctgtacgg
    15541 atcaggcatc aagtttttgt agtcgaacag ggtgtgcctt tgtctcgaga aatagacaaa
    15601 gatgaagccc attgtatcca cttcgtcctg tactcagata aaaaagaacc gcaaggaact
    15661 gtacgtcttt tgcctttaga aaacggaaaa atgaaattac agcggatggc gattctttct
    15721 gaatacagac accaagggtt aggaaaaata ttgattgaag aagcagaaaa ctttgctaag
    15781 aatcaaggct ataatacgat tttattaggg gcacagtcca cagctgaaac tttttacgaa
    15841 aaactaggat atacagctta cggcgatcct tttgaagatg ctggcatgcc tcatatttat
    15901 atgaaaaaga ctttataaaa atttatttcg aaaaaagcaa aaaacagcaa gcttttgaga
    15961 aaaatctctc actggcttac tgttcttttt tgtgtttact tacgcgacaa ttgcttgtaa
    16021 cggttatacc aaatatcaat atagccttcc gagaacgggc catctccatt attgatccag
    16081 tcaaccagaa tcttcacatt ttcttttagg atgaagtcaa tatcgttcgg gtaattcatc
    16141 attttgctgt gcatctcata ttcatcgaca tccaatagtc gtttctcgcc gtcaggaaat
    16201 accttgatat ccaagtcata gtcgatatat ttcaaggcct catcgtccag caaaaaaggt
    16261 gaagctagat tacaataata ggaaaccccc ttttctctga tcattgctat tacattgaac
    16321 cagtattttt tatgaaaata aacaatcgct ggttcgcgcg ttacccatcg gcgtccatca
    16381 gattcagtga ctaatgtgtg atcattcacg ccgatcatag agtgttcgct tgtttttaat
    16441 accatggtat ctcgccacgt tcgatgcaat ttgccgtcgt gcttgtaact ttggatcgtc
    16501 acaaactctc cttcttttgg aactcccata gcaacccttc cttaagcagc attttgtgct
    16561 ttgtgtttgg gtgttcatta caccgacatc ataattatta tatcaataaa aatgagcttt
    16621 gcctagaaag gattcctatt gtttcgattt ttctctaact gatctaccag tttcatttgg
    16681 accttaggaa aaacgaccga atcaaatgca tcaggcttca accacttcga agttttgttc
    16741 tcggtaaatt cttgttctgc tccctcggtt gcccgtccgt aaaaaaggag aacatgccat
    16801 ttcagatgac taaaaatatg agtaacttct cctaaatgtc gtgtctgcca aacaaagagt
    16861 tcctgctttt caaatgggag attttgctct tcttctgcta ctaggtcatc aaacagatcg
    16921 atttctggct ttgtttccca ttcttttttc aatcgctcat actcctcttg tgtcacttcc
    16981 atcattggaa atgtccacat attcgccagc aatttttggc tatctctttc ttcaaaataa
    17041 tacgctccag aatgattttg aagagctgca ctgatataat aaacgtcttt tggctttgct
    17101 ttttttgttt ttacaggaaa actcgtttgg attcctcgtt tgttagctaa acaaaaggct
    17161 tggatcggac aagtctcaca ctttggagaa gttggtgtac agatagctga acctagatcc
    17221 atcattgctt ggttgaattc acctggatgc ttttcatcaa tgattttccg catcgcttca
    17281 tcaaagattt tcctgctaga agcttttgcg atgtctgctt caatgcaaaa caatctgctg
    17341 actactcgca tcacgttgcc gtcaactgca ggttctggaa gtccaaatgc gatacttgca
    17401 atcgcgcctg tcgtatatgg tcctattccc ttcaatgagc taatttcttc aggcgtttgc
    17461 ggcatttccc catcaaattc tgacatgatc tgtttagcag cagcttggat gttccgggct
    17521 cttgagtaat aaccaaggcc ctcccatgct tttaaaagct tttcttctgg agcatttgct
    17581 agctcttcaa ttgtaggaaa ccattccatg aaacgataaa aataatcgat cactgtgtct
    17641 actctggttt gctgaagcat gatctcagag atccagatac gataaggatc acgattatag
    17701 cgccaaggca ggtttctttt ttcttgttca taccattgaa tgaactgatc ttgaaattct
    17761 tttgtttctt tatctgtcca tttttcccat tctttattca ctttcctctt ccttttccgt
    17821 aatgaatcgc tggatcatat ccaaatcaaa tcctttttga tataagcttt gctttacttt
    17881 tagatttctt tttttaggtt caaaccttaa atttcttcgc catagtttat ctccttgttt
    17941 gactaaataa tcatattcta tttcttgatc tgcttcttgc ggaagatcag ctatcgcttg
    18001 ttggatcact tcttgagaaa agcctttcgt catcaatgtt tgttggattt ttctcaataa
    18061 ttctttttgg ctttttccgt gattttttcc aaacgctttt tcggctgtct gatgtgcatt
    18121 ttgaacttgg tcttcaaatg tatattggct gagcgcttgt tcaatgattt caggtttcaa
    18181 acctttttgc tgcatctgct gcgctaattt ctttggtcct ttgtcactta agcgcatatt
    18241 cgtgcgcaca taactttccc catagaccaa atcatcgaca aggtccaatt cttttaagcg
    18301 ggctacgatt ttatttcgat cttcttgaag gatttccttt tcttttaagt atgttcggat
    18361 ttctttttcc gatcgcaact gataacttaa gtaattcatt gctaggtgaa aaccgaaatc
    18421 agctccgcta ctcttcttga tctcttggat agtttcttcg tctaattccc gtccttttaa
    18481 taatcgatag cgtactaata catcttcaga tacttgtaaa gcttctcctt cagataaatc
    18541 tacgcgatag aaagggcctt tgcctttact gattctcgta acggttaaca taaacatcct
    18601 cctcgaacat atattctcta tcataccaca gaaaatcccc gtaatcaggt attatatgtt
    18661 agaataaacc acagaaaaaa gaaaaaggga gatacttatg gcagaaacaa ttgtcaaaac
    18721 tggacaaacg gttcctttga aaatcaagcg tttaggcata aatggtgaag gaatcggtta
    18781 tttcaaacgg ttgatcatat ttgttccata tgcactacca aaagaagaag tactagtgaa
    18841 aatcacgaaa gcaactcctc gctatgcaga aggagaatta ataaaagtca aaaaaacaag
    18901 caaagatcgc gtagtggcac catgtcctgt ttactatgaa tgcggtggct gtcaattaca
    18961 acacttagct tatcaagctc aattggattt taaaaaggat ctgcttttgc aagcattgga
    19021 aaaattcaaa ccagcaggct ttcgatctta tcaacttttg ccgacgatcg ggatggatga
    19081 gccttggcat tatagaaaca aagcacaatt tcagttacga aaaaataagc agactcaaaa
    19141 aattgaagct ggtttatacg aagcaaactc tcatcagtta gtccctataa cagactgctt
    19201 ggttcaagaa cctcgaacac aaaaagtaat gaatactgtt gttcaactat tgaataaatt
    19261 tgatgcaccc atctatgatg aacggacaaa cagtggaatc ttccgcacaa tcatggttag
    19321 aatcggtgtt aaaactgggg agttacaagt tgtttttatt actcgcagta aaaaattccc
    19381 gcaaaaaaac gcaatgatcc gtgagatcaa tgaacgactg cccgaagttg tttcaatcat
    19441 gcaaaatgtc caggcacaaa aaacgtctct cgtgatgggg gatgaaacga tccacttatg
    19501 gggaaaagaa gccattgaag aacaaatcaa tgaagtgact ttcgatcttt ctcctcgtgc
    19561 cttctttcaa ctcaatcctc agcagaccga attgctttat ggcgaaggaa tcaaagcatt
    19621 agatgtccag cctaatgaaa cgatcgtgga tgcttattgc ggagttggga cgatcggctt
    19681 aagtgtagca aaacaagcca aagaagtacg gggaatggat accgttcctc aagcaatcgc
    19741 tgatgcaaaa aaaaatgcgg aaagattagg atatcacaac actcgatatg aagtcgggac
    19801 agcagagcaa gttctgccta aatggttgaa tgaaggtttt cagcctgatg gtatcatcgt
    19861 agatccacca cgtacgggtt tggataacgc attgatccaa tcattattga aataccctcc
    19921 aaaaaaactt gtttatatct cctgtaatgt ttctacactt gccagagatc tggttaattt
    19981 aagtaaaaag ttcaagattg aatatttaca gtccgtcgac atgtttcctc agactgcacg
    20041 ttgtgaagtt gtcgtgaaga tgacgagact cgctgactaa atgaaagagg ttgtgaaaaa
    20101 gtctgccctg tatcaagtaa taagaacagc caaacaaaaa tagctcttca tgttttatga
    20161 atactgtact tgtgaccgaa gagttaacag gaggacacta tttatttaaa aaaagggttg
    20221 tgacatagtt tttgtcacag ccctttttga ttattattgg atattcacgt ctatcacttg
    20281 ataaaatgca tttgtcgtat cgctgatatt ccatactgct aaaatcacat tatatccttt
    20341 tcgatcctgc ggaagtgtga tcgtttggtt gactgttttt ggtggaacag ttgctttgtc
    20401 gtctattttt gtgatcaatt caaaatcact aaatttcaaa ggttgattag gattccaagt
    20461 tggtttggta atgtagtaat cccaagtact tgtccgatgt tgtgctgtta aattccaagt
    20521 aatatttaat gttcctggat taaccatgtt tttatgccat ctgttagatg tctgttcatc
    20581 taaaggctca aagccgctga ctcctgcact agcaattttt ccatcaatga atgtattttt
    20641 ggtagcttcg atactttgcg gttcccattg tgctcttccg acattttgat ttaagtttcc
    20701 tgctgctgat gttcctaggt atgcacggct tcctggagaa gtaatataac catgcgcaaa
    20761 acctggaatg ctaaaaccga acaaccctaa actaactaac ccaataaaca ataacgtact
    20821 aatctttttc atgtagaaac ctccttcatt tgataaccac attgtaaacg ctttattgta
    20881 ttaacacaaa cattatgctt ttataatttt atgttgaagt aaaaaaggtg aaaaaagtaa
    20941 gatgcagatt ataacagaag tagacgagaa aagtacaaat agaaaacatg agaatcaaat
    21001 catatgatct ttttcaaaaa catcattcat ataaacaaat gttcaacttt ttaataacaa
    21061 caattatcaa tttttttctt acttcacaaa ctaaatacag gtcttgctgt gagccttcat
    21121 ggtaaactaa tattatagaa aggagttagt aaaatgaaga tgataaaaga tattcgtttt
    21181 tgggtttgtg ttataggaat tgtcatatta ggctttttat cagggttgct tagcggaaat
    21241 cccggaaaat attactattc tttgcaactt ccaccatttg caccacctag ctggatattt
    21301 gggccgatgt ggaccttact gtatatattg atgggtattt ctctttattt gttacttaat
    21361 cacaacaaca aaaaacagcg aaacaatctt gtcggtcttt ttgtgattca gttcattttt
    21421 aattttatat ggtctgctct attttttaat ttaagaaata ttttcattgc agcaatcgat
    21481 attacacttt tagttatttt cttgtcagtg cttatgtatc agttgtggct gcatcatcgc
    21541 ctggcaatgt ggctcatgat tccttattat ttatgggtac tattcgcaac tctattgaat
    21601 tattcaattt attttttaaa ctaagcgcta aaaagagacg tcctgtaatt taacacatag
    21661 aaaaagtgcc acctaagtta gattttttgt ctaatttagg tggcactctt tattaaccaa
    21721 actatttttt tctagtaccc acttgttgtt ccatactcat tattctggtc ataaccataa
    21781 ccattttgtt cctgtccagc gtaaggatca taactttgag tatcttgact tggtgtatag
    21841 tactgacttg tatcttctgt ttgctgataa taggtattcg gatcttggta ctgatcatat
    21901 gtgttttctg aataggaaga ataatcggtg tacccttcac tattcgatcc atcaccgttt
    21961 gtcgaatcgc catcttccga atcatcaaag aacatcattg aagcattctc atttttcaaa
    22021 ttaggtaatg tatcgcttgt ttttatattt aattgtttct tcagttcatt ttggatcgaa
    22081 agaagatcat tttttcctaa aatttgatag tagatatcat tgatcatttg tccttcgcct
    22141 tgcaactggt cttgtttgat tgtttcaaac gccggggtgt agttcgtcgc tacatcaagc
    22201 atgtcatccc aatctaagtc cgtcgtcaca ttgttctcaa ctgcttttaa aatttttcta
    22261 taattgctta cgccatctaa acttaaaact ttattgacga ttttcgtcac tacttcccgc
    22321 tgacgacgtt gtcgaccgat atcgccttct ggatcttcgt gtcgcattcg agaataggct
    22381 aaaccttttt ctccattcaa tgtttgttca cctactggta catggacacc atccaaagta
    22441 aagtcaattt tattattcac tttgatgcca ccgaccgcat tgatcaaatc ttccatgcca
    22501 tccaaattga tcgttacata atgatcaata ggaatatcga gcagttgctc aattgaatcc
    22561 atggccattt cgacaccacc aaatgcgtag gcatgattta atttatctac cgttccataa
    22621 cctacgatat tcgtataaat atctcggtcc aaactgatga tcgttgattt tttttgtttc
    22681 ggattaactg ttactaccat catcgtatca gaacgtcctt gttccgttct tcctaatcca
    22741 ccggtatcta aacctaatag caaaaccgaa aaaggttctt tgtcatcaat gcttactctc
    22801 tcagaacgtt tggaggatat tcgattagag tttttagaaa ttttgtttac tgtttggctc
    22861 gcttctccca taaatctgat tccgtatgct gataatccgg caatcagcaa aacgaatacc
    22921 cctaacaagg ttaatagaac tttttgccat cgcttcataa tacacctctg tttcacattt
    22981 tctaaaatat tgtctcatat aactgttcaa tcagcaaatt cactttttaa tcataagaaa
    23041 actttaaaaa acatttttgt gtaacgatta ttatttttta aaatttctca aaattcttaa
    23101 ctatcatacc ataccgtgaa aaagatcgaa agaccgaata cacgcagttc gcaatcgttt
    23161 tatctttttt tatgtatttt ttccagaaaa agcgaacaac aaggtatttt ccaaataatc
    23221 gtgcagtaaa atgatcgcac atacctttcc catcttttta acagtctttg cctaccgttc
    23281 agattatcat tcgatttcac gacaatttct tcctatgact ggtccttttg ttaaacccat
    23341 cttctattcg ttttatttcc agctcactgc atggctctta aaatgactcg aactagtgat
    23401 tgcattacca tcatctttta atggatagat atgctcctct tcaaagtaaa acgattttct
    23461 tttaaagtaa tagaattgac atggtaatga gtatgtatac tagtggtttc acaaatggtc
    23521 ttttattcaa catccaaaaa ctgaaatttc tttaaaatcc attactaacg aagaaaatgt
    23581 tccttgcttg ataattagtt aggacatgtc aagtgaacaa atgcatttac ttagccaaaa
    23641 aatctaaaaa cttcataaat agacaaaaag acacgactct ctaagaagtc gtgtctttat
    23701 tcaacaaatt attataattt agtcacttca gcagcttgag gtccgcgtgc accttcaaca
    23761 atcgtgaatt ctacttcttg accttcttct aggctcttga agccgtctcc ttgaattgct
    23821 gagaaatgaa caaatacgtc atctccgccg tctactgtaa taaacccaaa tcctttttca
    23881 ttgttgaacc atttaaccgt tccgtgttcc ataaatgtat atcctccaaa aaagttcttg
    23941 caagtaacct tgcaagaaca ttatacccaa tttagaaaac gctagcaagc tttcgctctt
    24001 ttattttcca aattattttt aagactgata tgacggagga aatggttatt ttgctgcttc
    24061 gaaacgtttg tttacttcat cccaattaat aacattccag aatgctgcaa tataatctgg
    24121 tcggacattt ttatatttca aataataagc atgttcccaa acgtccaaac ctagaattgg
    24181 tgtctttcct tccatcaatg gagaatcttg atttgcagta gaggtaatag ctaattttcc
    24241 attttccatt acaagccaag cccatccaga accaaatcgt ccagcggctg cttttttgaa
    24301 ttcttcttta aaagaagaaa aatcaccaaa agcttcatta atcgcttctt ttatttctcc
    24361 tgtaggttcg ccaccagcat ttggtgccat gatttcccag aaaaatgaat ggttagcatg
    24421 tccgccacca ttgttacgta cagctgtctt gatatctgtt ggaatagcgt ccatatcaga
    24481 taataattct tctattgttt tttcgcctaa ttctgggtat ttctcaattg ctgaatttaa
    24541 attcgtcaca taagtattgt gatgcttatc atgatgcaga tgcatcgttt cttcgtcaat
    24601 ataaggttcc aatgcgtcat atgcataagg tagatctggt aatgtgtaag tcataaaaaa
    24661 attcctcctc aatgttttct taataatgtc tacattacaa agagtaacaa agagaaggaa
    24721 attcttcaat tgttatgcct agtcgtcttt ttcactttga cctacttctt cttcaaaaac
    24781 ctcagtttct ttaaaaataa acaacttgct gaaaatatag ttcgcaagga taacaataat
    24841 ttgagtgatg attttcgcca catactcatt gctgttcata acattgatca acaataccat
    24901 tgcccccatg tccaatccgt aagaaaggat acggtagaag aagaacttac cgcattcaat
    24961 aagtgcatgt gtaaaattcg gtgattttga atggaatacc caaagtttgt tcgttacaaa
    25021 agcaaacaat acagaacaaa accacgaaat agaattggat aaggccatac ttaaatcaaa
    25081 atattggcgt gcgagagcaa aactaacaaa atttacaatc gtagccaaac caccaaaaaa
    25141 gagatagact agtatctccc aatatccttt tgcttctaaa tatcctttta actgtcgata
    25201 ctgcttcatg agccaaccta ccttattaaa aattcttatt tatcatactc tatatttcaa
    25261 taactttcat ctttatttaa aaaaattaat tttttttgat cctttgcctt tgtctctgct
    25321 tggatcgtca catgtgtaat accatatttt aattttaata aagaaattat acgaggatat
    25381 atcttttcaa tctcgcttag tagctgatct tcaaaatcaa tatgtccgtc gaaaataatt
    25441 atcttttcgc tatactgcca aatatggaca tgatgaacat cctttacccc atcgattgct
    25501 tcaatttctt gttccatttt ttcatagtcg atagcaggtg cactttgtat caaaatagcg
    25561 gcagcttttt tgatcaccat tatcgcttct atcaatatat atatcgaaat gatcagtgta
    25621 ataactggat cgatccagta gatatcgaaa aaacggatca aaattgctcc gataatcaca
    25681 cttaccgaag acagtgtatc acttaacaaa tgcaaataac tagattttat attcaaactt
    25741 tcatctgctt cttgtcgtaa taataatact gagaacaaat tcgcgagcaa tccaataact
    25801 gctactgtta gcatcaaatg actatttatc ttttctggtg aagtgaaccg cttgaatgct
    25861 tcaacggtta aaaacaagga taatatgatc aatatgaatg agttgatgaa agcactcaat
    25921 atttctaaac gctggtagcc atacgtacgt tttttatttg cttcttttcg ggccattttt
    25981 tgtgccccgt aagaaaaaat aattgccaat gtatcactaa ggttatggaa agcatctgaa
    26041 acaagagaca aactccctga cataatccct ccaataaatt ctgataaagt gatcaccgca
    26101 ttcaaaacca ctactccaaa tagttttttt gatgatctct gtggctgtgt aggcactatt
    26161 ccttcctcca ttttatttat attggatagt ctttagacaa gaataacatt tttttgatat
    26221 ttcaccaatc attcagctgt ttccttaaag cacttgtatt tcacttgata cttcgctaga
    26281 attgcaagtg atgacactac tagatgagga gtacttatga caacaaaaca attaattatc
    26341 agttccttta tacgattcaa agacctaaag gaggcgagaa atgtcccatt ttggaaagcc
    26401 attgtctatc ttttagtttt gagtataatt atggctttgc caatctcttt tcaagttttt
    26461 caagtcttaa acaacattca attggatggg caaaagattg ctactcgcat tcctgatttt
    26521 acaatccaaa acgggcagct tcaaaccgaa gaaaaagaag gattcattta ccaaacaaac
    26581 tccattatct tcacatttga tccagaggga aaacgaacag aacaggacat ctctactgat
    26641 ttgatgggga attttgttag tgtcgggatg ttaaaaaaca aacttatcat agctcttcct
    26701 aatacaggca cgacttctgc tttgttgaac aataaccaat tagaacttcc ctacaccaat
    26761 gaatctttaa aaaacttaac aggtaagcag ctccgttctt ttttaagtga agcatctata
    26821 ccactttgga taaaagtcct gacatttata ttttctattt atcctagttt tctgaatctg
    26881 ctgatcactt tgctatttac caacattgct gcttttctct atgcacgctt tcgactaaca
    26941 aaagctactt ttcttgattg tttaaaaaca ttgatctatt caatttcatt gcctactata
    27001 attgcaacaa tcttgatggt ctttcttcct tcttttgaca ctagcgcctt cattgctttt
    27061 gcaggaatct ttatttttgc tcaagctgtc aaaggatggg caaaaatatc gatcaattaa
    27121 gaaataaaga caactcgtta tttctacttc aatagaccct tttagaggtt gtgacatttt
    27181 tttgtcacag cccctttttt atttgaaaaa tatttctttc tcaaaaaaca agcaaggcgc
    27241 ttcaaagttc ttcttgtatg cgctacactt agagtaaaga aaggcgggaa attaactatg
    27301 tcagaaagaa tcatttatgc agatcgtcca tttcgtgatg aatttatcga agaaatccat
    27361 actatagcgc ctggttatat ctttaaaaca gaattacaac cagaggatct acaaaatgta
    27421 gagatcagtt tgggctggaa ctcagactac caagaaaaac tattagcctc ttctaccttg
    27481 aaatgggtcc aatctatctc agctggtgtt gatcagctac cccttgagac tttttcaaag
    27541 aaacatattt tactttcaaa tggtagtggc atccacagtg aatcgattac ggagcatgtc
    27601 ttaggaatca tccttgggta ttctcgtggt ctttttcaag cacaaaaggc acagagtgac
    27661 atgaaatggt taggttcatc ggtccattac caatctatcc aagggaaaaa aatactaatc
    27721 attggaactg gccatattgg aaaaatgatt gcaaaaaaat gcaaagcatt agacatgaac
    27781 tgctatggaa tcaatacatc cggtcatcca gcagaaggaa tggaaaagac tttttcttta
    27841 aatcaactaa aggaagttct tcctgaagta gatttcgtag tcaatatctt acctctaacg
    27901 gaggaaacaa ctgggctctt tgatcagcat atgtttgaac actttgatcc aaaatctgta
    27961 ttcatcaatg ttggccgtgg tgcttctgtt aagacacaag atctggtgca ggctttaaat
    28021 aaccagcaac tttcctttgc agcattagac gtttttgagg aagagcctct tccacaagat
    28081 catcctcttt ggaaaatgga caatgtactg attacttccc acattgcagg tttgacacct
    28141 gattttcaaa agaaattaat ggctattttt ttaaccaatc tgaaaagcta ctttgccacc
    28201 catgagcttc aaaccaatca agtaaaattg tctgcaggat attaatagct gaaaacaata
    28261 ttttcaaaag aaaaaagaga aggtgacaaa aattgtgtca ccctctcttt ttttattgtt
    28321 ttttaaagtg ataggttggc tcctccaggt tgggattata atcaacaagt aaatcatatc
    28381 ctttaaagaa ccattcatct gcttcctcga taaaaaaaag tgtcccatca atttccactt
    28441 tctttacagg atgttctggc tgttcaactg acattccaat cgaaaaacca tcatgaacct
    28501 ctgttttgcc gtagactttt ccaaaaaaac gtattccgta agtttctggc agatcaattt
    28561 ctgagtcaaa ccacttttgt gcttttggcg ttaccgttaa tttcatccca acaacctact
    28621 ttctcttttc tttctattga ttcatcttat cgtctagaat atccacatcg cctgtctctc
    28681 caataaccag tagattatca ttttcacgaa catattcatc tgctgcagga gaagcgataa
    28741 ccgtttgatc tactcttcgt atggcgacta ctgttaagtc aaaacgttgc ctgaaattta
    28801 attcagctaa tgttttgttg aaaaatttag gattggagac tcggatttct gctaaagaat
    28861 atttatctga caactctagg taatctaaaa tattctttga cactaaatta tgcgcaatcc
    28921 ttattcccat gtctcgctcg ggatggacga cgcgatcagc tccgatcttt tcaagtactc
    28981 tggcatgata ctcattttgg gcttttgcta aaatatttgg tacgcccatt tcctttacca
    29041 tcagagtaac taagatactg gcttgaatat cttctccaat agcgacaatt acatgatcga
    29101 aatttcttat gcctaatgat cgaagtgtca tttcatcctg tgcatttgcc acaacagcat
    29161 gagtggctat attcatatat tcattgaccc gatcttcatt actatcaata gccagtactt
    29221 cttgaccagc ttcgatcagt gttcggcaga tacttccgcc aaatcgtcca agacctacaa
    29281 ttgcaaaatt ttgtttcacc gttttcctcc tttagtatct tcttttctca gtataacaaa
    29341 gcctttacaa aaacaaaagc aagcatcatt attgtaaaaa agccaagaaa aataatagtt
    29401 attttctcag cttttgatta tcctctttat catcgttttt atttcaccaa accatgtttg
    29461 aatgcataga tagcggcttg tgtacggtct tccacttcta gcttagccaa tatattggaa
    29521 acatgtgttt ttactgtttt cagtgtaata aacaattcgt ccgctatttc ctgattactt
    29581 tttccttcag aaatcagcat taaaatttca ttttcccgat tcgttagttc ttcatgtaat
    29641 acaggctcat ttcgccgact cattttttcc atcatttttg ttgtcacttc tggttctaat
    29701 actcgctcgc cacgttgcgt tgcccttata gcatcagcaa tttcatgtgc ggtagaggtc
    29761 ttcaacaaat accctgctgc acctgcttca atggcaggat aaactttttc gtcatcaata
    29821 aaactggtta cgataatgat tttagcttct ggccaatctt ttaagattgc ttttgttgat
    29881 tcgataccat ccatttcttc catgactaag tccattaaaa tgacgtccgg tcgaagtgtc
    29941 atcgccttct catagccttg tcgtccattt tcagcttctc caattacttc aatgtcttct
    30001 tgaatagata aataaaaaga gacgcctaag cgcaccattt catggtcatc tactaataaa
    30061 acttttatca tccttatcta tgcctccttc atcaaaggga ccttgatttc tacacttgta
    30121 ccttgacctt taaaactaat gatttttact gttccaccaa taccagctac tcgttctttg
    30181 atattgttca atccataact tcccgttttc gtttcattca tattaaaccc tgttccgtcg
    30241 tcaataatac gtaacaaaag attattgtct attttgtgca aatatacctc taattcattg
    30301 gctttagcat gtcttaatgt atttgataac aattcttgaa cgattcggaa caagtgatcc
    30361 tcaatggaac tagatagttt tacatcttct acatcccatt tcagcgaaat ctggatttta
    30421 ttttgcaatt ccttcaataa ctgctcgata ccttgcttta aacttttctc ttctaaattg
    30481 actggacgta gatgaagaag cagcgcacgc atctcagact gggaagcgtt gatgatctct
    30541 gctaccattt tcaactgctt gcgaaacatt tctggtgtct cgcttttttc tgcttgctcc
    30601 gtcaatgccg acatcatcat cattgctgca aacaattgtt gcgaaactga atcatgcagc
    30661 tcacgggcta atcgatgtct ttctaattct aaaatttctt ctttggtttg tccatctaca
    30721 tattggggac ggctcgttac aatttgtaat tcactagata cttcaatcat tttttctttt
    30781 atcttagtga tttccttatc aatatctttg atgtataaat cgtccgatgc acttgggatc
    30841 ggaagaagca acaatttgga ttcataattg ccagaagaaa gtgcacgcag cttttcttcg
    30901 attttcccgt attttgtctt ttgaataagt gataataata gaaagacaat taatcctaca
    30961 cctaaggaaa tggctaacaa atggaagata agcggaacat ataataaacg tgcacgcagt
    31021 aattctcccc accaatgact ttgattgctg gcataaaaat aagtgaaaag tgaaaataga
    31081 ataataagga aagccgcaat ccccgaataa acagctagca tcgctctgga tatttttccc
    31141 atcatattcg tatcacctcg acatctccaa tcaaggtatt agtaataatc ttcaatctac
    31201 gagggttttc atcataatca ctgctgtaga ttttgatttg ttcatttttc aaagaaaatt
    31261 gctcttcttc aaacaagaca tttcctacta atgtcgcatg ctctaatcgg atggctacgc
    31321 ccaaaggtac taagatccgt gttctgccta tcccttttct tacaatcaca atattatcat
    31381 cttttggcaa aagcgtattg cccaaatcta tgatagtatc accagaaatg acggcgatgt
    31441 tgatatcgtc ccattcatag acgtcgtttc caatacgttg gttaccgaat aattgctgtc
    31501 ttttccgctc attattatgt gtttttaatt gctctgtttg gatcatcatg atctgtttct
    31561 ttcgccaaaa agcatttttg gttaaatcta tccctgaaat ctctacacct ttcagaccaa
    31621 taaataaaac ggcaaataca gccatcatcc ataaagcagg actgttgact aagctaaaaa
    31681 aaatgaccaa acttcctaat atcaattgaa aattttgaaa tttagtacga ggatgtttgc
    31741 gcatagctag atatatattg aaaataccga atatcactaa aagcaaaagt ccagtattgt
    31801 ttacgatttg ccaaacagca aataacagca gtagtgcttc aactaccaca aaaaaacgcc
    31861 aagaactatt catcgctctt tctccctcct gacacattct catttcatca tacctatttt
    31921 tttaactgtt ggctatagga ctttagatgt aagatcgttt gttgaaatct aaaatacaca
    31981 aagagaaaaa gatctagagt gtatcatcag taagatgaac tctctagatc tttcagtcag
    32041 actacgcttg ttctactttg acgattttaa ctaacatatc gccacctgga gtagcaatag
    32101 ttacttcatc atttttacgt ttgccaatca atgcttgcgc aataggagaa tcattggaaa
    32161 ttttgccaga aaacggatct gcttcagcgc ttccgacaat cgtgtactct tcttcttctc
    32221 catctggtag ttcgatgaat gtaactgttt taccaattga tacctcatct gcatctacac
    32281 cgtcgttatc gatgatttga gcaaaacgaa tcatattttc aattgttgtg attcttcctt
    32341 caacaaatgc ttgttcatct ttcgctgatt catattcaga gttttcggaa agatctccga
    32401 aaccgcgggc aattttgatt cgttcaatga tttctccgcg tttgactgtt tttagttctt
    32461 ctaattcttt ttctaatttt tctttacctt ctagagtcat tggataaact ttctctacca
    32521 tgtgtaacgt ctcctttttt cgataaaata ccgcaaaagg taatcttttg aggaatatag
    32581 tccaaaaaag attaccacgc ttttcttcaa atgtaaatgt ttttctaaaa atgatcaatg
    32641 tctagagcct gtttttctaa gaattattta catatttttc cactaattcc atatgttctt
    32701 cataagtttt agcgaaataa acatttccag tgctcgtatc tgctacgaaa taataatagt
    32761 cattttctgc tggttctaat actgccttga ttgcctcttc gcttggattg tcaaaaggtc
    32821 caggtccata gccggtattg gtatacaaat tatatggtga atctacagct gtatcttcat
    32881 acgtcactaa ttctttgtgt tcacctaatg cgtataaaac agagatatct gactgaagtg
    32941 gcatatttgc tttgattcgg ttaaagaaga cttgtgcaat gtttttacga tcattttctt
    33001 ttacgccttc tttttcaact agagaagcta gagttaaaac ttcttgtaca gtcaaatttt
    33061 tctgcttgat agctgaataa tagttttgca tgaccgtatt cgtcttgttc accatttcaa
    33121 tcactagatc cttaagcgaa gtactcgtgt aataatcata tgtggctggg aataagtaac
    33181 cttccaaacg gtattttgtg ttctcagctt tagatgcact ttccaacagt tctgggaatt
    33241 tttggtgcaa ctcgttaaag aaagtctcat cattcactaa atctaaaaat tctttcttgt
    33301 ctttaccagt taccttcgct acccgttcag caatctgatc gatatcgtac ccttctggaa
    33361 tggcaatttt cccatctgca acttttgttg gctcagaagt accgccttct tgcaactgtt
    33421 tgccaatctc gtcaagagtc atattaggcg ctaactgata atagccagct tgaaagccag
    33481 ttaaattttt aaattttgta taataattga aaacaatccc gcttttaata atattgtcct
    33541 tctccaaaat ctcaccaatc tgcttgttag aagagccact cgggatctcg acttggacta
    33601 attggtcatc tgatttatct aaaggcttca acccgctatc tacataacga tacacggtaa
    33661 atccaagaaa tccgccaata atcaataatg taagagcaat cactaaaatg atcttcctca
    33721 caatcttatc ttctttttta cgcagatttc tccgcttgtc gattatatct gctgtagctg
    33781 ccttactggc ttctttttta tctttacttg ggtgttgttt gtttcctgac aaaacaactc
    33841 ctccttacta tcaagttcgt aactttattc attatacatg atatttgaat tggcgtgaat
    33901 gattttattt aaatattcta tcaaaaatgt aagtagtaaa tccattacta taagattaaa
    33961 ataaatgtta tcaagataca agctatttta gctttattct ttctgtaaaa aagaaaggag
    34021 actaaataaa ctattatgaa aaaaggaaaa catgctaaaa aaagaaaata ttcattttta
    34081 atgatcgttg tcgtgttgtt tagccttagt gcgttctcta caggaactat tcttggatgg
    34141 caattttaca ataatgaaca ctctcagata gttgcccatg ctcaatcagc tcaaaaaaag
    34201 ttgatactga ctaccaatga acaacagctc gttgaaaaaa tcgatcaact attaagaaaa
    34261 aatgattata ttggctcaat ttacgtaaag aaaaataatc gcatcatcat ggaaaaaggc
    34321 tatggctatg cgaataaact cacgcaacaa ccaaatgatc cttctcttta ttatcagatt
    34381 ggttcggttc aaaaagcatt gactactctt ttgattttaa aacaagtcga gcgtgggcac
    34441 ctttctttag atactaagct ggctcaattc tatccacaaa ttgctggcag ccagcaaatt
    34501 actatccgtg acttgctgta tatgcgctca ggcttgcacc gaactgcttc tccaaccata
    34561 ccgatgactg atgaagaagt cgttcaattt gcgattcagc acttaaaatt tacagattat
    34621 cagacctctc attatgattc attgaacttt accatattga ctggaatttt gatcaaatta
    34681 acaaatcaac cgtatgaaaa attattgacc gatgatatca tcgaaccttt aggattacag
    34741 catacaaaat tttatgacca agtcaaaaat tcatccaatc atgcactatc ctatcaaatg
    34801 tctaaagagg atgattactt taaaccactg acagaatctg aaacagagat ccataacgaa
    34861 ttagggactg gtaatatcag tatgtccgtc tatgatttaa atacgctgtt tacacaagtt
    34921 ttatctggaa aactagtttc caaagactta ctcttctcat tatggcgtga aaatgcgaat
    34981 ggacatccat atagtggtgg ggtatatagc ggagataatt atatcctgtc gcaaggaaat
    35041 atcgactctt ttgatacatc tgctatattt agcaaagatt cacaggatgc tatcatcatg
    35101 gaaagcaata taaaagctga caaaactatc aaactgccta ctgctgatct aagaaaccaa
    35161 atctatgacc tgatggaagg aactactaag ttggcaaata gctgatatga tgaaaagata
    35221 gcaaatacta cttctttgaa tagcatttgc ttaaaaaaag tactcgacta aacgtaggaa
    35281 agagtgtggg acaaatctaa aaatcaattt ttgctacaga ctttaattcc aaataaacag
    35341 aacagaaaga ggtgttcttc tttctgttct gtccaaatta ttctaagatt tctctttacc
    35401 aacattattt gacaaagggc ctattaaatc taaaatagta tctttagatt gctaaaatat
    35461 gtgctagcaa atcattcatg gcttgttctt atttttaatt ttatgcaata aaaaagaaga
    35521 acaattagcg tgattataga aataaataat gagattttag aaaaaaatgt gtatgaatac
    35581 ctgattgtta ctttatttat tccactttca ctttttactg taacaaaagg acctccttgt
    35641 tttagtactg tagcattttt tgaattaaca gtaaccgtta atcctttata ccggaataca
    35701 ggtaaaacta ttgttttgcc ttttgaagat ttaaacgtaa atgtaatttc atctttattt
    35761 ggagaaaagc tactataaat aacttgacca tcttctgtaa cttgatggtt tttcaaaagc
    35821 aattgatctt ttttatagcc tataggtaca taatcaaaat tctcaaattc ttcaagttta
    35881 ttttccattt gttcattaga ataagttcct tgaaaa
//
LOCUS       DAYYCW010000014        34773 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000014 DAYYCW010000000
VERSION     DAYYCW010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.014"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..414)
                     /locus_tag="ACHAYA_000697"
     CDS             complement(<1..414)
                     /locus_tag="ACHAYA_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604526.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENV"
     gene            complement(709..1044)
                     /locus_tag="ACHAYA_000698"
     CDS             complement(709..1044)
                     /locus_tag="ACHAYA_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8604527.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1031..1315)
                     /locus_tag="ACHAYA_000699"
     CDS             complement(1031..1315)
                     /locus_tag="ACHAYA_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8604528.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1371..2894)
                     /locus_tag="ACHAYA_000700"
     CDS             complement(1371..2894)
                     /locus_tag="ACHAYA_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8604529.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(2887..4062)
                     /locus_tag="ACHAYA_000701"
     CDS             complement(2887..4062)
                     /locus_tag="ACHAYA_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8604530.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4066..4251)
                     /locus_tag="ACHAYA_000702"
     CDS             complement(4066..4251)
                     /locus_tag="ACHAYA_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604531.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4217..5911)
                     /locus_tag="ACHAYA_000703"
     CDS             complement(4217..5911)
                     /locus_tag="ACHAYA_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8604532.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(5908..6381)
                     /locus_tag="ACHAYA_000704"
     CDS             complement(5908..6381)
                     /locus_tag="ACHAYA_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8604533.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(6450..6605)
                     /locus_tag="ACHAYA_000705"
     CDS             complement(6450..6605)
                     /locus_tag="ACHAYA_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFM8604534.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(6739..7119)
                     /locus_tag="ACHAYA_000706"
     CDS             complement(6739..7119)
                     /locus_tag="ACHAYA_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8604535.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7123..7338)
                     /locus_tag="ACHAYA_000707"
     CDS             complement(7123..7338)
                     /locus_tag="ACHAYA_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604536.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7341..7748)
                     /locus_tag="ACHAYA_000708"
     CDS             complement(7341..7748)
                     /locus_tag="ACHAYA_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFM8604537.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8014..9489)
                     /locus_tag="ACHAYA_000709"
     CDS             complement(8014..9489)
                     /locus_tag="ACHAYA_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFM8604538.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(9479..10327)
                     /locus_tag="ACHAYA_000710"
     CDS             complement(9479..10327)
                     /locus_tag="ACHAYA_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFM8604539.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10364..10726)
                     /locus_tag="ACHAYA_000711"
     CDS             complement(10364..10726)
                     /locus_tag="ACHAYA_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604540.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(10727..10885)
                     /locus_tag="ACHAYA_000712"
     CDS             complement(10727..10885)
                     /locus_tag="ACHAYA_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604541.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(11026..11650)
                     /locus_tag="ACHAYA_000713"
                     /pseudo
     CDS             complement(11026..11650)
                     /locus_tag="ACHAYA_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(11647..11958)
                     /locus_tag="ACHAYA_000714"
     CDS             complement(11647..11958)
                     /locus_tag="ACHAYA_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604542.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12002..12295)
                     /locus_tag="ACHAYA_000715"
     CDS             complement(12002..12295)
                     /locus_tag="ACHAYA_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604543.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            12489..13136
                     /locus_tag="ACHAYA_000716"
     CDS             12489..13136
                     /locus_tag="ACHAYA_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8604544.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13197..14342
                     /locus_tag="ACHAYA_000717"
     CDS             13197..14342
                     /locus_tag="ACHAYA_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8604545.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(14423..14728)
                     /locus_tag="ACHAYA_000718"
     CDS             complement(14423..14728)
                     /locus_tag="ACHAYA_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM8604546.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(14753..15184)
                     /gene="ruvX"
                     /locus_tag="ACHAYA_000719"
     CDS             complement(14753..15184)
                     /gene="ruvX"
                     /locus_tag="ACHAYA_000719"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM8604547.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15181..15447)
                     /locus_tag="ACHAYA_000720"
     CDS             complement(15181..15447)
                     /locus_tag="ACHAYA_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM8604548.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(15702..16427)
                     /locus_tag="ACHAYA_000721"
     CDS             complement(15702..16427)
                     /locus_tag="ACHAYA_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM8604549.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(16788..16858)
                     /locus_tag="ACHAYA_000722"
     tRNA            complement(16788..16858)
                     /locus_tag="ACHAYA_000722"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16824..16826),aa:Gly,seq:ccc)
     gene            16975..17727
                     /locus_tag="ACHAYA_000723"
     CDS             16975..17727
                     /locus_tag="ACHAYA_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8604550.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            17743..18222
                     /locus_tag="ACHAYA_000724"
     CDS             17743..18222
                     /locus_tag="ACHAYA_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8604551.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(18473..20053)
                     /locus_tag="ACHAYA_000725"
     CDS             complement(18473..20053)
                     /locus_tag="ACHAYA_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM8604552.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(20046..20789)
                     /locus_tag="ACHAYA_000726"
     CDS             complement(20046..20789)
                     /locus_tag="ACHAYA_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8604553.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(20965..21741)
                     /locus_tag="ACHAYA_000727"
     CDS             complement(20965..21741)
                     /locus_tag="ACHAYA_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8604554.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(21989..22825)
                     /locus_tag="ACHAYA_000728"
     CDS             complement(21989..22825)
                     /locus_tag="ACHAYA_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM8604555.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(22836..23765)
                     /locus_tag="ACHAYA_000729"
     CDS             complement(22836..23765)
                     /locus_tag="ACHAYA_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM8604556.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            23957..24310
                     /locus_tag="ACHAYA_000730"
     CDS             23957..24310
                     /locus_tag="ACHAYA_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8604557.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(24384..25040)
                     /locus_tag="ACHAYA_000731"
     CDS             complement(24384..25040)
                     /locus_tag="ACHAYA_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8604558.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            25266..27200
                     /locus_tag="ACHAYA_000732"
     CDS             25266..27200
                     /locus_tag="ACHAYA_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8604559.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(27286..28509)
                     /locus_tag="ACHAYA_000733"
     CDS             complement(27286..28509)
                     /locus_tag="ACHAYA_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8604560.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(28503..30374)
                     /locus_tag="ACHAYA_000734"
     CDS             complement(28503..30374)
                     /locus_tag="ACHAYA_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM8604561.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(30395..31513)
                     /locus_tag="ACHAYA_000735"
     CDS             complement(30395..31513)
                     /locus_tag="ACHAYA_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8604562.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(31596..32429)
                     /locus_tag="ACHAYA_000736"
     CDS             complement(31596..32429)
                     /locus_tag="ACHAYA_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8604563.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(32422..33339)
                     /locus_tag="ACHAYA_000737"
     CDS             complement(32422..33339)
                     /locus_tag="ACHAYA_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8604564.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(33566..34690)
                     /locus_tag="ACHAYA_000738"
     CDS             complement(33566..34690)
                     /locus_tag="ACHAYA_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8604565.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        10425 a         7028 c         5687 g        11633 t
ORIGIN      
        1 cacattttcc aaatttgctt ccttataatt tgaagaacga ccatgtttta ctctgttata
       61 tattttccac caatctggtg tatcttcagg ccacccatct ccaaaaggtt ttagtgaagt
      121 caatgttaaa gaattcataa ccctaacacc attatccaaa cacattaatt cgctagattt
      181 aataacagct ttaaaatcag ttatgctttt attgctactg ctatcaaatc cgcaaagttc
      241 tttgaaaata atctcacact ccgaaccaat cgctagtagt agtttttgat attcaataga
      301 gaatgttttg aaattattct catcgagcat cacaaaatct gtggtattaa taaactcatt
      361 ttctaaatgt agataatact tccaataaaa atttgtaaac tctgctttgt ccattaaatc
      421 acctcttttg aattataaca aaaaaaacac gtccacaaca gacgtgttct ttgatttcta
      481 cacaatcagc ttgccaaaac catccaatca gtcctagcat gcctgtatta aaattgaacg
      541 ttcatcatct atcatctttc ttagtccagc atgtttgcaa ccacacctac aattttcagt
      601 ccaacaatag atttcgacta gctaagtctt gcaaaattca ggcagctaac ccttcattta
      661 ctaccttaat tatactaaat acccacaacg tttacaagtg taatcattct aagaaatctg
      721 tttagctatg atcgttgtat aatctttaaa cgccgttcct tcattgattg aaataatttt
      781 ataactcttg tttttccaag ctactttcat atcgtttgtt agttcataat cttgatcgta
      841 tcgaataata aacgtcaacg ttccttcaag aatggtacct acactagcaa gcacgtcttt
      901 tagcatttgt gaacgcacat tagcccaaca ttcaaataca gtttcttcac tatccaccat
      961 ctgcccatct tcatctttta cccgttttgt ctttttaaac ttgactctct cagtcaattc
     1021 atttacattc ttaatgattg gcataataag cacctctcat ttgttgtact aatgcttgta
     1081 ccgttactgg tatccgttcg ctgctggctt cttgacggtt caaataccaa tgttgagtaa
     1141 gtaatgacac cgccaaatca aattgctgat aattatccat atctttatcc gttgcgctac
     1201 tatcaatagc actttttata tattcgcctg ctgtatcaat caattgttga ataaagtcat
     1261 catcaatagt gtgatcgatc cgcatacttt tttttatgct atccaaaatt accattttat
     1321 ctctcctaat gaaaaaaagg cagtgggata aaccgccgcc caatattttt ctatactttt
     1381 ggtgttacaa ctggcgtaaa ctcaatatac actgaagcat tttgatcaat tttcttataa
     1441 tcgttgcgaa cgatcactgc aagtccttgt gagtagtaat cgaatttttc ccattgagtc
     1501 gttacttgat ttctacgagc aacaaagata gattgtgcaa tatcccccat aatcataggg
     1561 aatgttccag ctttaggatt ggcaaacaat gtatctgcaa tcaatactac tggcataccg
     1621 aataatgatt taccactagg tgctgtcaca tcgggttgta aaatgtaacg tccatctgaa
     1681 tctttcaatg tatctaggtg gtaatatccg ctttggttta gaattaccat tttatttaat
     1741 gctgggtcta atgccacatt gtatagttgt ttcaaatcgt ccaacgtagc agccgttttc
     1801 ttagtgaatg tttttaataa atccatgata tgcttattgt cagtgttttc taccaatttc
     1861 gctaactgat ctttgacctc ttgtacaata ttcactgctg aatcttccac aacctcattt
     1921 gataaggcaa tcttaccagc acgagtttct actttataat caactgaagt aaacatttca
     1981 gcgtcaatat caccaatttc agcaagttca gcttttgttg ctaacactgc ttgttggtta
     2041 gttgctactg gatatttacc ttgtccattt gatactgttt ttactgttgc atattgagct
     2101 aagttataat ttgaacgttt caaatcaaag acttcaccga ttacttcttc gggaacaact
     2161 gccgctgcat ttacagtagt gactccatca cgcacttcgc cttgtgaacg gatatattct
     2221 tcatacccac gcaattctgt tttttcgtta tcgattaaag ttttttccat gttattactc
     2281 tccttttgtg ctttagaagt ggattctaag ctattgtttg tttgttttgc ttggttgtta
     2341 ctcataaacg attcgtaaga acgcttattt acttggacat ttgatgaatc ataagcggga
     2401 acggtcacga cactaatttc attaagtgct ttgattttat ctattgatcg ttcaattgtg
     2461 ccatcttctt ttttgtcgaa tgaatctaat ccaagcacga acccaaagga catagaatca
     2521 acaacccctt ttgagatatt ctcatacaca tcatttgaat aggtcgtgtc ggttagctct
     2581 gcttcaaaat gtaatcctgt atcatcgaca ttcaatttca acgtacctgc tttgacacta
     2641 gctagtggct ttgaataatc gtgtccatat aataagaaca catttgataa atccacctct
     2701 tttaatgctt cgggtgtaat cacttctaca aagccgccta aatcttttga cggttgcccg
     2761 aatttcaacg catagcctga aatagttttg ccgtctgttg gttgtttttc attttctttc
     2821 tctactttgg gagaatcggc tgttagctca gcttcttctg tcagccgttt ttccttttct
     2881 tcttcattat tcaactggtg taactccttc ctgttctttt aattgatatt tctccaacgt
     2941 atctaaatac gtaaagttca aacttgctag taaacgatcg ccaccatcca tggggggtag
     3001 ccccattttc gaccgcccct cattaatcgt gagaagtgac cctttgacct gatccaatac
     3061 attttttact tttgtttcag gatcagtctc tagtaactga tcggaattaa agcggatatt
     3121 ggtttcaagt ttcgtatcta attcactcac aaacacatta aagtaatgaa tcagcgtact
     3181 ttggatatac tggagattac tttgaacggt gctagaatgt tcattttcaa cgcctaaacg
     3241 ctcaatcggt acaccaaacg ccttggcaat ttgttttgtg ttccaatcat tggaattgat
     3301 aagcttcaaa acatcggtgt ttatttctaa tgttttgtag tccattgttt catcaagaat
     3361 gatcgttcta agagcattat ctccactact tgaaccgttg gcttcttcaa acttttcacg
     3421 aattgcgttc tttgctgatc cgtctaaatc tgacttatga accttcaaaa tacctgaccc
     3481 actgacacca cgagtaaaga aattgtgcaa tgtgcggttg ccagcttgtt gaattttcag
     3541 ttcatcacgt aacgcataaa gtggtggtaa tcctgttaaa ccatcttggg tgaaatattt
     3601 gaagtgcaaa atatcgctag acttcacacg tcttttttta tcaccaatct gataagacaa
     3661 cgtaccatta tctaattgag taaccgttac acttgagtta ggtaataagt gaatttcttc
     3721 taccttttca ccgttgcgtt taatctctgc aaagctatta ccgtttagca acatattgac
     3781 cgccaaagca aactttaaat gccaaccatc catttctgaa ttaggtttct cgtttagtaa
     3841 cttcaccagt tcattatcag cttgtggcat attgtttttg accaattgaa tcggacttga
     3901 agcaatatca ctagcaataa ttcgaacggc tgcaaacaca tcactatttc ttaatgcacg
     3961 aacgcttgta aagctggtgg tgtaatcatc cgattggatt gataccacat gttgtaggaa
     4021 aggatctccc gttgtatttt gtctcgcttt aaaaaatgcc atcttttaac ctcccttctg
     4081 ttctgtattt agcatgaccg ctaccccaat aagcactatg cccagcgcta acaagccata
     4141 gtagacattt gttagtaagg tgatagcaac caacacacac accaatccaa gaagtaaaag
     4201 aacggtatga atgtaattag aaagaaaatt cttcacttgt ataaaattca ttgtctgctt
     4261 tcgctccttc cgttgtggtg aagtaatcca ttgcaaacac ataggcattg attaatgcag
     4321 ctattggatc aattttgtta ctgttctttg atttattaat ctgaatgccg ttgttatccg
     4381 tttttaaaat agcgttgttc actgcatggg tgagaatcgt atttttattg tgaataacgt
     4441 tgccttcata aacttgttct ctaaaagttc gggtcggaac gtttagagta atcgttcctt
     4501 gtcttacttc taacattggg tagttggctt tttcagcttt actgataagc gaattagcgt
     4561 tatacgggtc ataacaaatc cccatacatt ccaaatcatt ttcttgaatc aaatcttgaa
     4621 taaactgaaa tacttggtca tagtccacga ttccactttc taattgagta atggaacact
     4681 caccagcttt ttctagttct cgataaggca aaccgtcgcg cttttctttg tcttgaagcc
     4741 catatttggt ggctacaaag ctatgtgaat cacaataaag ttcgccgtta tctagcggta
     4801 cgatccatga aacgctagtt aaatcatctg ttttagataa atccactccg atataaacgg
     4861 gcttgcctgt aatatcttgt ggttctactt cggttgcctg ccagtcttca gcaatcatat
     4921 agctgtcctc actcgcttgt ctccataaat tgaagttttt caccaataca gaattaaggt
     4981 tattttgttt caaagcaagg ttcacatcat cttgaatggt tggaatcatt acttttttga
     5041 tttcttcact ttcaaaaatc ggattggctt tgatccattt ctcttgatcg tgaatttctt
     5101 cgggatcgtc caattcccat atcgctataa agtacctgtc ggcttgttct tcgcctttta
     5161 gcacacgatc taacaataag tattcctcgt acataggaac gtttaaatca agcccagcgg
     5221 tactaataac acacaataag ccgttctttt gttgggtcat tcctgactta atgacgttgt
     5281 acgttttacg agtttttgct tcgtgccact cgtcaagaat tgcggttgtt ggtgcaaacc
     5341 catctagcgt gctggtttca ctagccagtg ccatagcgaa cgaattagag ggcaagtgct
     5401 gaatcttaga gttcataatt ttcagttgtg gtcttaaaaa cttactagac ttgaccacgt
     5461 ttctaagtga attagacagc atatcatagc ccaatttagc ttgtttcaaa gcgttactta
     5521 caaacaatac ttgccttgct tctgctggtt cttgttccat aatcaacgca ttggcagcca
     5581 tgccacttgc gatatacgtt ttcccattct tacggctcat actggtaaac gctcgattga
     5641 atcgcctgta attcccttct ttagtacgcc agccataaag actgcctaga atgaattttt
     5701 gaaatcctaa catcttgatt gctttaccgt ccgtagatgg tagcaactca atgaatttaa
     5761 tcgctttggt ggcttgttct tcatcgaaca cataagggaa gctatctgac ttagaacgct
     5821 ccaaatcgct tatatgacgc tcacacgctt ggataatttt cttgggtgca atcagcttac
     5881 ctgataggac tttctcaatg taatcattca tgaattcatc aactctttga acgggtcttc
     5941 gggttcatca tttttaacct tgttcaaagc tagttttgat cgtgattcta aggtcatacc
     6001 tagtgaggtg gcttgttttt tcaaatcact catagcttgt gattgaaccc gaaccgctgg
     6061 attggctttc tttactccgt tttcgcatgt gataaacgta cctgattttc ttatctcggc
     6121 ttcggctgtt ttcattcgag caaaggctaa acaataggct accaatgaac cataatctgc
     6181 ttcactcaat ggataatctt tttttaatgt tggtacgata cgattccatt cacttctagc
     6241 gctcgctgcc atccagtcag ggggcgtgat atttattaat ggttgctgct tgaacaattc
     6301 ttctctagcg tccacacgga ttgctttttc ttcacttgat atattgcctt ttgtatcgtg
     6361 taatatttta gggtgtccca tgttgtcctc ctttcagaca aaagaaaaag gaacgtaagc
     6421 aattaagctc acattccttc gtttttcgat cagaattttt ctttatctaa tttatctata
     6481 taaatgattt ttccgttctg catttgaatg gtcacttgac caaaatgagg taactcaatg
     6541 gtgcgctgtt tccctgtttt atcagtaata acaattgtat ttttcaataa atctcctact
     6601 ttcattatat actttttcac ttgtgattac aagtgtaatc gttatttgta attaccctta
     6661 tgccttttca caacggttat ttgtaccaaa atgagtaggc atcgattgtc agtaagtttc
     6721 acactatgcc cccttaactc aaagggtact cttttctcgt ctttgtcttt cattggctgt
     6781 cttggaatta tggtgtgcat ggcataaaga acgaaaatta gtttcatcta accgtctatt
     6841 ccaatcatct tttagttcaa ttttgtgatc tacaacatca gctttcctta tcaagccttc
     6901 ttctaggcaa tcctcacata ctggatgatt taaccgatac aagtatgaag cctttcgcca
     6961 tgttctactt tgatagaatt gaaaatattt accttcgctt tgctttcttt cttcacgctt
     7021 aattgccgtt tgctttgctt tatgcttccc acaatacttc tgcctataat caatcaatgt
     7081 tttacatcca gcatgattac attgtttttt aactgccata gattaccaac caaagcgatc
     7141 tcgttcgtca gaatcatctt gttcccattt tgatttgtat tcaatataag gcgaaactat
     7201 cttcaagcac gttccgtctt taaaattaac tttagtgatt ttatcttcct caaaactaat
     7261 aaagtctaca tcttcttgag tgtttgaata ctcaatgtgt ttctcaccat cgaagtatct
     7321 aatactttcc atatgcataa ttattctacc tccaataaat tagttatcaa ttcatcatct
     7381 gttagtagca aagccaagaa cttatcatgg atgtttaaaa acaaattatc ctcattcgat
     7441 gtaagctctc gttgtctcgc tcgatcatat aggttaagca gacaattaaa ttcatccatt
     7501 agctcacctg tatcatcata gttgccacta tctaccaatg atcccactac tagcgattga
     7561 caccacgcta ctgactcctg cagccaatca ggttcttctt ccattatctt taatacttct
     7621 ggatcaaatg tggaataatc ttctgttacc aatcccacac taaatgccag cgtgttcatt
     7681 agaaaccgat ccatttcatc tgtatattga aagttcggtg ctttgtttgt tgaaataata
     7741 ttgagcattt actttcctcc ttgattcgta acacgtggta acacctacgt aacaggtacc
     7801 cgttaccact caatcccaac ggggacaagg gttctagcgt tttggtaaca ggtaacgggt
     7861 aaaatccagt aacattattc cccagcatat tagctaacta tttcaaaagt tttaaggaaa
     7921 taaggtgtta ccccgttacc aaattgctgt atcccttggg gcagtaaggg taaactagta
     7981 acgggctagg tgttactgac ccgttactac ccgttacttt ttgtactttc cagctcgatt
     8041 tttattttca taggcatttg acttattgta aaatcctcgt cgtttatttc tttttggcag
     8101 ttgcttccgt tcccaaccag gaatactgct aataatcaag gcaatctttt tagtttctgc
     8161 atctgatcga ccattcaata aatcctttgc ttgtttatca aaaacagctt ccaaaatatc
     8221 agcggtgagt acgctatcaa ctaaataaaa ctcttgttgc atagtagctc cattatagaa
     8281 atgctgtaaa tttttctcat tacatttact gtcaggatag ttctgataat aagaacgtct
     8341 cgcccacgct ggagcttctt cccatttata cgggatttcc atttccaaat atttagtgat
     8401 tgcttcttta attggattct ccatcttggc atcctcttga tagtcttttg caatttctaa
     8461 gacttcttta tcttcatcgg gactaaaata gattcgttgt cctttatcaa aaagcacctt
     8521 tgcttcagct aacacttgaa gaaaatattc atcctttgtt ttaaacggat tcatctttgg
     8581 cttattctta caaggcaaag gataaaaacg gcggtttcct gtatcatctt tcaaatactg
     8641 cccatcattc gacgttccaa taaatacaca ttgccttgcc caagctatga cattccgacc
     8701 ataaggaggt ctaattttat cgatcttggc actgatgaag ttttttatct gttcaatttt
     8761 agttttgttc attgaagata attctccaag ttcaataatt acattgcctt gaagctgcat
     8821 ataatcatct ttcttttcac ctagcgaatc taatgaatcg gtgaaaaaat catccgtgta
     8881 aagtaatgct ggaacggtac tcttaccaat cccttgcggt ccatccaaaa ctggaacgat
     8941 ctcaaacttt attcctggtt tatagatacg tgcgactgat ccgactatcc aacgttttgt
     9001 tacttctcgt gtgtaaaggt tgtcgtctac ccctaaaagg tcaataaaca gtgtttccag
     9061 tctaggctgt tgatcccatt taactttttc gatacgttct tttactgggt tgaaaacatt
     9121 ctttctagcc acatttacta ctgctttgtg tattttgttg tcatcaaaaa ctacattgta
     9181 gttttcttca atatatgccg ttaaggcatc ctcaaaactg tcttcccatt ctccagcggt
     9241 acgattgatc caaggagaat cttttaatag gtgaatgtat ccgctaaatt cgttataagc
     9301 aattgttcca gctaaattgc tatcattggt taaaatcaat tctgcattca caacactgtt
     9361 acttttaatc tgatcgtctt tattgcatct taatcgtgac atccaggttt tcaattcaat
     9421 gatattgtct tcacgctcgc tggcttcggg aaagtctaaa atactatcta atttatgctt
     9481 atgagccatg agctttcccc ctcaaatctt ttttaatcat cgatcgaata atttgattca
     9541 cttctttatc ttttagcggt ggttcattgt acgcatttgc tgtataaatc aattctgcta
     9601 aagtttgatt atctacaccg tgccatagta accaaccgca taacttagtt aaatgatcgt
     9661 ttctactacc ttcgccagcg ccttgaacaa tcatatctaa tttttttgct gtccctgttt
     9721 ttgctacggg tcggtattga gtctttattt cttgcggttt ttttagcagt aggttcacta
     9781 gccaatcggg agctggtgca atattattta gagatacgtt ttcaataggt tcatatgctc
     9841 tcccaacact tcttgaaata atcatagttg gcgctattgg aactccttta gtgataatat
     9901 caatcccgct taaactttta cctttatcat cacgcccgaa gatatcagca aatgcacctg
     9961 tttcttgttg gataattaca ccatcaggaa ttttaaagaa gaaatggatt cctttacctg
    10021 gtgtgatttc cgtataagta gaaggaaatg gatcatacat tgaagcaagt tttttataat
    10081 tctcaacgcc gtttcgtgtt tcagaatgtt gatctaggtc aactaccacc atttgatgat
    10141 cttcaagcat taacccaata tttctagttg catcttccgt ccaccattca ttaatttgat
    10201 tagaatcgtt agttgcatca ttccaaccac tagagccttt tattggaatt ttagttccag
    10261 gagataaggg ataaattgac catcccgact tttgtaattc cgttgctgct tgataattat
    10321 ttgccaataa acctctcctc ctttttcaaa atactggtat aaatcattca gacacctcca
    10381 tgagcttcaa ctgctcaagt tcgtatttca acccatcccg aacaaattca gcacctttta
    10441 attgattctg atactttacc catgtattaa atgttttcac ccttgcttct tgcatatcag
    10501 taatgtttaa ccatgcggtt aaaatctgat tttttagctc gttgatttct cgacaaatta
    10561 ttttgtgttc tgcatgtttt ttgagtacat acaaaactgt tagtttttca ttgaatgaat
    10621 ttccaaagta ggttttcccg atattatttt ctagttcagc gatcccttga ttaatttcct
    10681 ctaattcaag gctatcgagt tggtttaata gttttgtttt gtacatctac ttatgccctc
    10741 cagttcttaa ttgatagtcg tactcgtcat aagcagtgaa gagatataac gctaatcctg
    10801 caaagatagc tatttttgca attaatggta ataagccgaa aattccgccg attaaaatgc
    10861 ttcctataat taagtttcgt ttcataatgg ttccctccga ttttttaata tggtataatc
    10921 ggaacaaaga atatcgggtt ttatcccaat taagttgcct ttatcctgtc cgccaagact
    10981 gttatggata aggctttttc tttgttctcg atcatgttaa tgctcctaag ttgtctgcaa
    11041 ttcagcagaa cgattataaa ttttctcact cactgaaaac tgcaacccat atttttcttt
    11101 aacagtcatt aattctactg tctcatctaa aataggttca cgatcaacca acatttcagg
    11161 agtcatatct ttctttttta ccattttgga atgaccataa atagttgata cagctttatt
    11221 ggctatcaca ttcgctttaa tgtagtctac tggttttggt tcagtaatag cgtggcttaa
    11281 tttagtcatt gcttcttttt ggtgttcttt gtctagcatg cggaatactt gaaagccttc
    11341 aagtcctgtg gactggcgta gttctttgat gatgtcgaaa atccagtctt gaaatgcctc
    11401 ggcttctttt ttacggcttt tgaatgccat gcggtaaata ttttttttcg ttgatgatgt
    11461 ttacatcttg tgttctgccc aatgaatcga tgaccttact agtagtaacc ccatctttag
    11521 gcaaagagtg aatggctcta gtaacctgtt taagacctaa cgcttccgca acatctttcg
    11581 ccactgccca ccattcatca ttaatatcga caaatcttat aatgtgtccg ttccaaattt
    11641 cagttttcat tcaacgtcct ccagttcaag ccctagcaag ctcgctaaca actcaacttg
    11701 ctgatacaaa atttcttgat actcttcaat agaaagtcgt tctttaaact ctgcttgtac
    11761 taaatcgttc aagccacgca atactccgct taccgttggt tcttgtggta gcaatttttg
    11821 atagtccatt tgttcgtaca aacattcaaa cgtttcttct agcgctttgc cttccaagtt
    11881 tgctaattct gccgctgtgt cttctgtaag aattgttgat aatacttttt cgattgcttt
    11941 attttgtgtc gattccatta ttatttcctc ctattaatcc ctttgccccc acggcttgcg
    12001 tttatttttg gtattcttcc ataaagcgat cacattcatc ttgtgaaatt cttacgactc
    12061 catcaactat aatcaacttt aaaccgttag gaacatattt ctgcatcata gtattgtatg
    12121 aaacgttcat atacttagca gcttgtgcct tgttcatata ttttggaaac tggtactttt
    12181 tttctaataa tgaaagtaac gttccttgag tttgctctac ttgttgaaca actctatcgt
    12241 caataatttt aaaagctgga tcatcaagcg tgagtttgtc aatttttaat tccatattta
    12301 tcccccctcg ttagcataca tttctataca aagattttaa aaaaatgcaa gaggctcttg
    12361 aatacatttc tatacctctt ataaactcaa tataccatca taccttttca tttgcaagaa
    12421 ttttaacata cttttttgaa cttttatgtt tcacacatat ttaaatcgtg atatagtaga
    12481 gaaaatatat gaatggtggt gaaaatgtgg ctagaaaaaa ataccccgaa gcagtggaga
    12541 caggacaaag aattagactg gctagaattg aaaagggact ctcaatggaa caacttggag
    12601 gtaaattatc cccaccggcc agtaaaggtg ccgttagtaa ttgggaaaat ggctataatc
    12661 ttcccaataa caccaggctt aaacaacttt caaaggtatt agatgtatcc actacctatc
    12721 tattaaacgg atcttataca ttaagagata tgcatatgat gcccgaaatt aagcgaaatg
    12781 tgcttgaaaa gcaattgaaa aaaactattg aaaaaaataa ctctataatt gattctaatc
    12841 tttctaatgc tatcaataac atcgaagaag ataacctgac tgtctcacag aagatttatt
    12901 taacaaacgc aataaaatat cttaaaacag gatctcgaat tgaatttcct gacgggagta
    12961 ccccaattgc ttatctcgca gctcttctaa atacgctttt acgatatgac tcattaatta
    13021 atgattcaga aaaaaataca gatgagaaaa aagagtatca agaattttat gaaaatgatc
    13081 ttctaaaaga gttcacagct cttgttactg agctaaataa acactattca aaataataat
    13141 aaactgcctc ctccaaccgc ccccacggct tgctacacta tggagggaaa actattatgg
    13201 caacattcaa gcaatatgaa actaaaaaag gtaaattttg gctctatgaa gcctatcttg
    13261 gaatgaacaa aatgactggc aaacctgata aggttcgtag acgaggttac aaaacaaaaa
    13321 aagaagccca gcttgccttg tcacgcttgc aagtggacta cgataaaaac ggtattaaaa
    13381 aagcgaacaa cgaaacgttt aaagaagttt atgacttgtg gctagaaaca tataaaacca
    13441 ctgtccgaga agccaccttc atgaaaactg aaatcaagtt tagaaagtgg attcttccaa
    13501 aatatggaaa tcttcgtgtg aatgaagtca cggtaaaaaa agcccaacaa attgtaaata
    13561 gctgggctga aactactgac caatacaagg tacttcattc aacttctaag cgtattttta
    13621 gctacgctat caatcttggg attattgaga ctaatccact agacaacgta ttaatgccta
    13681 aaagaaccac cactaagcat aaagaaaagg tcaaagttta cagtaaagaa caactgatca
    13741 ccttgtttga ttacctaaac agcaaacctg aaacttatcg aaacgacttt gataaaacgt
    13801 tactacgctt tctcttttat agcggggtta ggatcagtga agcccttgca ttgaattggt
    13861 cagacattga ttttcaagaa aatacggtca ccatcaataa aacactaagc caatccaaaa
    13921 atggctataa aatcagtgat cctaaaactg attgtagtgc tggggttctt ccactagatg
    13981 aaagaacaat ttctatatta aaaaaatggc agattaatca gcggaaatat atgctgacac
    14041 ttggtgtaac tgatccgact atgatttttt gtggtatcta taaagaacta atcacacacc
    14101 acgctattta tgcaagattg attactattt gtgaaaaagc tatggttcca tttctcggtg
    14161 tgcatgtagc aagacacact catgcttcaa tgctcttgga cgctggagct actatggtag
    14221 aagttcaaac tagattacgg catacaaaaa ttagtcagac gatcgattgt tacggtcact
    14281 tggcaaaaga aacgaaagaa aagactgttg aaaaattagt acaacactta aatattgact
    14341 agatggcata caatatggca tacagtaaaa aataaaaaaa ggagaaaccc tatcatatca
    14401 aggtttctcc tagctttata gtctattctt cttcttccgc catgaatgta ttgaatactt
    14461 cttcaatcat gtcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat
    14521 cttcgttttc gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag
    14581 caggatataa aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagtagga
    14641 tttcatataa tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcat
    14701 ggtcgtggga atggttatgt tctgtcataa gattacctct tttcttttat tattaaaata
    14761 attgcttacc tgagccgtct aaataatttt gtaagatcat cacagctgct aatttatcga
    14821 tgactttttt tcgtttggct cttgaagtat ttgcctgctc tactagcata cgttctgcct
    14881 gaacagtggt taatcgttca tcttgataat caactggcaa ggagaatagt tctttgatct
    14941 tttctccgta tgacatggaa gcttctgccc ttggtccgat ggaattgttc atatttttag
    15001 gtaatccaac tacaaaccgg tctacttcat attgtgctac taattccttc aaccgatcaa
    15061 agccaaactc ttcttctgct tcattgatcc gaatgatctc tactccttga gctgtccagc
    15121 caaaggggtc actgatagca acacctactg tcttagaacc gacatccaag cccattactc
    15181 tcataaattc actccatgat ttgagaggta gtccttgaca attacttcca tgatctcatc
    15241 gcgttcgtga cggcgaatca aatttcgagc atcacgataa cgaggaatgt aggacgggtc
    15301 tccagaaagc aagtagccga caatctgatt gattggattg tagccttttt cttccaaagc
    15361 ccgataaaca atcgctaatg tttcgctcac ttcttttttg cgactatcgt caaaaccaaa
    15421 acgtacggtt tcatctgtaa aacccatgat tctacacctc tttcccgcaa ggtaattcca
    15481 gcttaccttc tacaacctta ttttactcga agaaagggaa aactacaaac aaatattcca
    15541 aacttccgaa taaattaaga aacactattt gtatgttttt ttcctttttt ctgtagcttt
    15601 atcaaaagta ctcttttcat aaagtatacg ctatatttct ttgaatgtat gtttttacac
    15661 aattttttca taatattcat aaaaaattcg aatagctgct actaattttt tgtttttgtt
    15721 gcttgtcctg tctcataatt gatcgtgtag ccatcttgaa cattgtagat aaagacattg
    15781 aattcaattt ttttgtcttc aatgctttct gcttccaatt gcactcccct ggctaccagt
    15841 tcattcccct caaaatgagg agtgacacgg tagcgtacat gatgatttgt ttctttgata
    15901 tatgccgcaa tatcattttc gtgagcaagc atgtatggag cattcaacga acgtgtccct
    15961 gtcatcaagt ttttaatgtt gttattttga ccagtcagct ggaaaccaat taaatggctg
    16021 cgattatata accattgccc gtcacttaat ttcttttgtt tccaacctgt cgggtcaata
    16081 tacaaaggct ctctcttttc agtagggaac aaatcttttc caagcatagc attggcttgt
    16141 cctactctgt tcaatttatc aatatttgaa tagctttccc atgttccttt ggaaaggcta
    16201 agatcctctt ttgtaaattc cggctgattc ccattgattt ctatttcttg atgttttcca
    16261 tcatattctg gcacatccgc tacttgagaa gcttgtggtg tggaatcaaa aatagttgta
    16321 atctgctgaa agatatctga atctttttgt gtggtggaaa caccgaatgc ccctgcaatc
    16381 acagcagcga cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt
    16441 tactaacagc tccctaaatc ttaacatatg ttcccctata aataaatact agaaagaaat
    16501 agaaacagca aaaaataaaa cctgttttaa gatggcttaa aaatacaaaa aagagacgga
    16561 ttcctcgtct ctaatcagtc accactctat ctgtaaggta tttattatta ttcaaaaatt
    16621 ttcttcatac tcaagacaat acactgattt cacaaccgct tggtaaacgg tgcgcaaaag
    16681 cttgcatctg cggtaataag tccatccaag ttaaaaacat aagaagttgt ttttgcttgg
    16741 ctgttcttat tacttgttgc agagcagctt tttcacaacc tattttaagc gggtgacggg
    16801 aatcgaaccc gcgacatcag cttgggaagc tgggatttta ccactaaact acacccgcaa
    16861 tacaaaaaca gtatacgcct cttttattcc ttcctcaagt ttttttattc tttacgcttc
    16921 tggtatgttt tgtagtaaaa taaaactaat caatcaatta ttggagaaga tgctatgacg
    16981 ggaaaagaat tagatctgtt gctggacact tgtttactcg ctgggaaaat catgatggaa
    17041 agcaatgcgg agatgtatcg ggtagaagat acgatgagtc gaattgcttt agcttctgga
    17101 aatttccgtt tggtcagcta tgtcactcaa accggtctct ttattggtct tgatcgtacc
    17161 tcgaccatcc gaatggaaca aattaccaac cgttcgatta atttagaaaa agtagtaaaa
    17221 gtaaatgatc tttcacgtaa atatgttgct tcagagctaa cattagaaga actttactgc
    17281 gaactgcaag caatcgaccg agaccgttct ttctttcctg tctggttgca aatcgttagc
    17341 gcggcagtga tcagtggaac aattatggtg ttatttggcg gaaagatgac cgatttacct
    17401 gtgactttag tcattggagg attgggatac gcagcttatc tatatagttt gaaatttttt
    17461 agaatcaaat ttttatcgga atttttatct tctttattga ttggcatagc cgctatctac
    17521 agcgtacgtt ttggcttagg aagcaatcag gatctaatca ttatcggatg tgtgatgcct
    17581 ttagttcctg gagttcaaat tacgaacgct atccgtgatt tgctagctgg acattatgtc
    17641 tcaggagttt ctcgcggaac agaagcgatg atgactgcta cgatgatcgg ttttgcgatt
    17701 gcttttattt tccagctttt ttattaggaa aggaacacaa gtatggggac gatatttatt
    17761 cagttttctt ttagtttttt agcgactgcg gcatttgccg ttattacaaa tgtacctagg
    17821 cgttcattga tttgttgtgg tctttctggt gcttttggct ggatgatcta ctggatctgt
    17881 gttcaaatag gcggatctgc tgcttttggt tcattgcttg gttctttagg agtagcattc
    17941 atcagcgatc ttttctctaa acgtttgaaa atgcccgtaa cgattttcaa tattcccggg
    18001 atggttcctc tagtccctgg tggtttagct tatcaagcgg ttcgaaatct cgttactgaa
    18061 tcctatcgag aagcggctca ttatgcggtc caagcaatta tgattgctgg agcaatcgct
    18121 ttaggtcttg tattatcaga agtctttaac cataacatcc gtaatttcaa agtaaagcgt
    18181 gacgcaatca tgatgaaacg aaaaaaacgg ctttttaaat aaacgaagtg taacaaagaa
    18241 aaggttgtga aagagacgtt cagccttgag cgatgacaaa atatctcaca aacattgcga
    18301 caagtaaccg caggagcaat aagaagtaat tatgagtatt aaaaaaagtt ttgtaaaaat
    18361 agcttctcac atttttacat aaaattgact taatactgag aaactgactt ttgaacaccc
    18421 tctatctgta aaaaaggggc tgcgacatga tttttgtcac ggcccctctt ctttattcaa
    18481 attttttcca aaacgttcgt tgataataaa cgatccatag agcggaaagt attagaacta
    18541 aaccgatcac aggaatattc actaaccaaa atggataaaa caaagccaac attccgtata
    18601 agacgataag aataatactg atgatcaatg aacccatcat agttgcgact aaacccaaac
    18661 ttcctgaacc tctgttaaat aattggttaa tattggtcca gttcagcagc agcaaccgat
    18721 aatctctagc gaaataacgt agtgacaata agtagcagcc gaataaagaa ccaaatagca
    18781 agctagggat aaaaaacaaa ggcagctgaa aagacagtcc gcttaataat gcgatcccac
    18841 cagttaaaaa actctgcaat atccatccga atctgaactt ttcttttaaa tattggttca
    18901 tagagattgg caaagaacgg ataaacagaa aattttcttg atccaaagaa ataagattgc
    18961 tgataaaaga agtctgatta actgttaaga aagccaatgc gatccctacc aggaaaacaa
    19021 caccgattaa cttattatct aaatcagtta attttatttc gcctcctagg gcaaaagcaa
    19081 gaataaaaat gattggagtt aacaaggaag aagaaagaac ttgcataatc aaattaggtt
    19141 cttttactaa ttgagcattg taactaaata aaagctgatg cacattttgg ttctttttat
    19201 gggttctttt tattttgaac tcactgtttg aatcagtcag ttgctctgta agctttggta
    19261 ctatcagctg ttttacagca aggaaaaatc ccagagccgt gatagctagt attccccaac
    19321 ccaataaacc ttgttgagaa agtggctgac tggcagcatc atagaatggc aataatatcg
    19381 aaatcggatg ccgatctgtt aactcatttt caatagtccc cgtttgatga ttcatccaca
    19441 tgattccagc aaatgcgatc agcatcgagc tgccaagcaa gatactagaa actgtttttc
    19501 tatgtttttt aaacactgct gttcgagcca aaccaaaaac aatcaaacta gccagacaaa
    19561 aaagaataat caaaaataga gaaaacaaaa ggattgagat aatgatagta agcaaaatcc
    19621 ctagatcagc tcgccatccg gttaaaataa acatgactag aagcggaaaa acaaaaggaa
    19681 tgatggtcaa agcaactacc aaaatctttg ctgaaaaaac ctgttcttgt ccaaaaggca
    19741 gaggcaaaaa tacttgcagg tcatttcctt caaaaaagat attataaatg actgatatac
    19801 tttgagttaa acctaacata ccaaaaagtg ccacgtaata agtaaagaaa cctggcaatt
    19861 taacaaagtt catagcaaac atagcaaatc cgtaaatcag taaaaaaatc agccctgaaa
    19921 aaagattttg agacaaaaga tagatagtta atcgctttcc cttttttcct ttctctcttg
    19981 ctttattcgt gagctgtgga ttaacatata aaagattaac tcgaattaat tctaataatt
    20041 gtcgcttata catacggtcc atctcctgtc gcttgccttc cagccatttt caaataaatc
    20101 ttttctagcg tttcatcagg tgagccattc aataattcac taactgcccc ctgataaatc
    20161 aattcacctt tcttcaaaat agccagttta tcgcataact gttgtgcggt atccaataca
    20221 tgtgtagaaa aaatgactgt ttttcctttt gcggcatgag ccttcatcat ttctttcaaa
    20281 tcaaatgctg cttgaggatc aagtccttgc aaaggttcat ccaataccca aatatctgga
    20341 tctggcagca acgtgccgat caaaatcgtt ttttgacgca ttccatgtga aaaacttgcg
    20401 attgtttcat tttgatgact atacatatca aaaagcgctg tcaattctgc taaacgcttt
    20461 tctttttttt gtgtgtctaa ttcataggca gctgaaatca agtcccaata ttctccagcc
    20521 gtcaattgta aaaaaatatc tggggaatca ggcacataac cgattttttg tttgatactc
    20581 aatcgatttt ccgtcaattt cattccatct actaaaatcg ttcccgatga tggttcaata
    20641 atactaacta aacttttaat cgtagttgat tttccggcac cattatgtcc taaaaatcca
    20701 aaaatttccc cttgtcttat cgagagagac aagtctttta atgcttgttt tttcccgtaa
    20761 cttttactta cattgatgaa ttcaatcatt tctgtactcc ttttgtttgt tattactttt
    20821 tattgtaccc ttcgttattg gtggagtaaa tgtcaaaaag agtgcataac aaaaaaggat
    20881 agaaacgact aggctgctac ttgggttgtt ctcttctaca ggaaaacact cttctaacag
    20941 gtaagatatg agtctttgtt ttctttaaaa tgattcattt tctatctttt cttgcataaa
    21001 attctttagt ttatcaatac ttctctttcc catctctagt tgttctccat ttgcaaatgt
    21061 tactatttcg tttactctgc taatagatgt aatgttttca gtattaatga tataagcttt
    21121 taaatcttta cataaatact catattcgag tgatttttta tattttttaa ttgtgccgtt
    21181 tacgagaatc tcttcatctt tcaatactag gcctatttta tttttataac ctggcatgct
    21241 cattagataa aggatttcat cgtaatggat atagtgatcg ctattaccat aacggacagt
    21301 tgtccacctt tttttcgtag catgttgaac aggatagttt tctttttctt tcaataagta
    21361 actttggatg ctttttttat ttttttcata agtgaattct tttatcaaat aaccactagg
    21421 cgtaatcttt cgattaataa cagttattgc tttattcgga tgagacgtta gataaacaat
    21481 tttacacttt tcattctttt ctctaagcat ttcgcctagt tcgataccgc tataataggc
    21541 gttcaaatcg atgtctagga aaaaaatatc tgaggaatag atttcattat ttttaatgaa
    21601 ttcaacaaat tgtataacgt tcaatccctg aacgatctca aatttccaag gacttaataa
    21661 aattctggat tcttccaaaa tttctactag cattttttca aaaaccaaac tatcatccaa
    21721 aataaatata cgcgttacca taaaaagtac ctccactcga tattttacta tcaaaatcat
    21781 ttcatacaac ttttcaacat acatgttagg aataaattgt aattctcttc acaatctttt
    21841 ccaacacctt tgaaaccgat atcaaatact ttcaggatgc tgcttctaaa ataaatatca
    21901 aacaaatgaa tacatcatgc aaaaaaagcc agacaaccga cgtccttgcg tgtcagctgt
    21961 ctgagctatg tttatttatt ttgttctttt aaaaagaacg atacacatac tgatttttcg
    22021 gagcagagac tttctcagcg tttgtaacaa ctacagatgg gatttcacgc ttgaatggtt
    22081 gataatagtc tgattggatg gttccttcta ttttatacca ttcatcattt ttaaccgtta
    22141 ctccttccgg catatgcgtc aacaaaccaa agactcctga atctgccaca caatggataa
    22201 tgccgaatcg gaacaagaag atatctgtat tgctatcgtt cggtgtttgg taaacaaatc
    22261 cttcatagct gatttttttg ccaatgaatt cgctagggta attataaatt agctccataa
    22321 cttctaaata gttttcatct gtgatcttga tagtttgctt atccccatac ttttctttca
    22381 actcattcat ttggtctaag tagtctgatt tgttgaaata aatactggta tccggcttta
    22441 agtattgtgt ctgcatctcc ggatccccta ctgattcttt acttactggg aaattaaatc
    22501 ctttggcttc tacgatcgta gtgtcgagac taactgttgg aaacaacgtc cccacgatta
    22561 ctggcaaagc tagcagtaca taagcgattc cacgttgata aggttttgac aacccatggt
    22621 catgatcgtg ctcgtgtgcc ttttcttctg cttttccgtt tttgacccac aaatacagct
    22681 gaactaaagc caaaagagca gataaaatca ttgaaagaat agctaagtag cgataatgga
    22741 cattgatata ttgatcgagt ttgccagaaa cttgcaagta catcatcaat tcgaaatacc
    22801 cagataaaat caaaaaccgc agcatatttt ccctcctaaa taaataatgt gaatccaata
    22861 acgctaaggg caatcagtcc gatcagcgat aaaataaagc gtcctgtaaa atatcgtttc
    22921 atcatcagta gatttttgat atcgaccatt gggccaaaga ccaaaaaggc agctacaggt
    22981 gctgtgccga acagacttaa caaagaagat cctataaacg catctgcttc tgagcaaaga
    23041 gacatcgtaa aggccaataa cagcatgatg ataatggcta aaatctttgt tgagccgagc
    23101 gttagcataa ttctagttgg taaataagtc tgcatcgcag ctgcgattaa tccgccaatg
    23161 atcaaataac gacctgtatc aaaaaattcg tcgattgaat gtgtcaacac atgcccagtt
    23221 tgatggatca gctgattttt tggttcattc ttctcacttt gttcttcttc atggacatga
    23281 tgatggtgat tttctgctac tttagacgtg attttttctt tcaatacagc gtctttttga
    23341 aaataggcaa gccagacacc aatcacaagg gcaacaaaaa agctgcctat cacccgccag
    23401 cttgccatct gccaagaatt gccaaatgcg ataaaagtgg aaaaaatcac gattggattg
    23461 atgatcggag cagtcagcat aaaagcaaac gctgtgtgaa ctggtacgcc ttttttaaca
    23521 aattgatgaa cgatcggtac gatgccgcat tcacatgaag gaaagaaaaa tcctaagatc
    23581 gaaccagtaa aaatagagaa gatacgattt tcaggaagcc atctcttcac acgttctggg
    23641 gtcaaaaata cttgtaaggc gcctgagata agacagccta ataatacgaa aggtaatgct
    23701 tcaataacga tcgataagaa aatcgtactc atctgcaaaa ccgaatttgg aataaaagaa
    23761 aacatgagtt tcctcctttt tatttctagt tcattcgctt tgcaaatata cgcttttatt
    23821 caaaaaaaac aagttgctct gtaaattttt acacaactct tattttttac caaagcactt
    23881 caattgactt tttctgttat tcccattaaa ttaaggtctc atacatatca cttctatcat
    23941 aaaagggaga ataaaaatga aacaaaccac taacttgact gaagttcaag acatactgca
    24001 acagtcatcc gttgccgtca tttatctttc gatgcctaat tgcagtgttt gtcacgctgt
    24061 tcgtccacga ctagaggaac ttctcacaca ttatgatatt ccggcacttc atttggatgc
    24121 atttgaaaca ccagaagtag ctagtaaatt tgaagtgctg actgcgccag ttgtcctgat
    24181 ttttcatcaa ggaaaagaag tctttcggca agcacggttc atcgacttta aaaaaatccg
    24241 ttatctacta gatgaactga cagcagaaga tgattccctt tcttatgaag aaatcttccg
    24301 aacaaaataa tcgttgtcca aacagaaaaa agcgattgaa caagtgaaag ttttgccttc
    24361 atttgttcaa tcgcttttta tttttattct gctgtatttt cggaatgcgc atttgcgtca
    24421 gaatgcaaga aatagatcaa ggtttgcaat tcattcgtca aatcaacatt ttggatcaat
    24481 acatctgacg gagcattcaa tcgagcagcg gtaaagttca agattccttt gattcctgct
    24541 tccgttaact gattgactac ttcttgtgct ctttcagcgg gtatcgttaa aattgctact
    24601 tcgatttgtt ggatcttgat ttgttctgac atatcttcca ttggataaac ggggataccg
    24661 tctacgatgc gcccaacgat ttcctcattc acatcaaaag cacaactgat acgtatgcta
    24721 ttactttgat ggaatttgta ttttagtaaa gcacttccca agttccctac accgatcagc
    24781 gcaacatttg tcaattgatc gtcgtttaat gtctttgcaa aaaagtgcat aagatcttct
    24841 acatcataac catacccacg ttttcctaat tcgccaaaat aagaaaaatc tcgacggatc
    24901 gttgcactat ctacttgaac ggcttcactt aattcagttg aagacacttt tttctttcca
    24961 gcattattta aaatccttaa atagcgataa tacaaaggca gtcttttagc tgtcgccttt
    25021 ggaataatat gatctttcac gaaatgacct ccacgtattg tctaattaca tctttgattt
    25081 ctccatcata gcagtattta taggaaaaaa tcaattccat tgctcagata ttgtgaaatt
    25141 ttattcttta ttcatttatt tatcatttct gcaaatctcg cagtataatg gaatttatgg
    25201 tagactacta attgaaaaat agttttttat ttgaggagaa ttcacaaagt agaaaagagg
    25261 attccatgat attattacaa gccaatcaga ttgctcgcca ttttggtgca gaagtcttat
    25321 ttgaaaacat acatttagaa atcagtacag gcgctcgtgt cgctttagtt ggcagaaatg
    25381 gcgctggaaa atcgacttta ttaaagatca ttgctggaat tgaagcacct gatgaaggaa
    25441 agatcgctaa aaataaaaca gcgactctcg gatacctcgc tcaggacaca ggacttgatt
    25501 ccaatgaaac agtttggaac gagatgctga aagcttttga acaagttaga caaatggaag
    25561 agcggatgcg cgagctagag attttcatta gcgaaggtac gccagacaca cctgcttata
    25621 gttcttattt aaaagaatac gaccggctgc aacacgattt ttcagatcga aacggctatg
    25681 gttatgaaaa tgaaattcgt tccgtattgc atgggtttaa attcgattct tcattttatg
    25741 atcagaaaat cgatacttta tcaggcggac agcgcacacg tttagcttta gctcgcatgc
    25801 ttttgcaaca ccctgacatt ttgattctag atgaaccaac gaaccattta gatatcgaga
    25861 cactttcctg gcttgagaat tatctgcaaa gctatactgg tgcattattg attgtttccc
    25921 atgaccgtta ttttttagat aaagtagtca atgaagtcta tgaattaagc agaagaaaaa
    25981 tgcgccatta caaaggtaac tattcgaaat atctagattt gaaagcagaa cagttagcaa
    26041 gtgaatggaa ggcttatgaa aagcagcaaa cagaaatcca taaattagaa gacttcgttg
    26101 ctcggaacct tgttcgcgcc tctaccacaa aacgagcgca aagccgcaga aagacattag
    26161 aaaaaatgga ccggctggat cgtcctcaag gggatgaaaa acaagcaaac tttctattca
    26221 gtatagaaaa gccttctgga aatgtagtct tgcaaatcga agatgccgct atcggatatg
    26281 ataatgtcac cttatctgaa ccaatcaatc tagatatccg tcgtcaagaa gcgattgctc
    26341 tagtaggtcc gaatggtatc gggaaatcga cgttgctgaa atcaatcatt caccagatcc
    26401 cttttatccg tggaaaagaa acgctaggga caaatgtcac catcggctat tacgatcaag
    26461 gtcaagctga tctgcataca aacaagacga tcctagcaga attgtgggat gagcatccta
    26521 ccactccaga aaaagagatc cgaagtgctt taggtgcttt cttattttct ggcgaagatg
    26581 tagaaaaacc aatccctctg ctaagtggcg gtgaaaaagc acgggttgct ttagccaagt
    26641 tagcgatgaa caaagaaaat tttttgattt tggatgaacc aacaaaccat ttagatattg
    26701 acaacaaaga agtattagaa aatgcattga tcgattatga cggcacactg ctttttgttt
    26761 cccatgaccg atatttcatc aatcggatcg cgacaaaagt cgtcgaactc tctgaaaatg
    26821 gcagcaaagt ctatctagga gactatgatt attatttaga gaaaaagcag gaagaagaag
    26881 aaatcgccga attgttggca caagatgagc cagaatctgc tcctgctccc acgaacaaat
    26941 ctgattatta ccaaagcaaa gaacagcaaa agcttatccg tagccttcaa cgaaaaatta
    27001 ctcaggttga agaagaaatg gcgcggatcg atgaatggat cgatcagttg gaacaagaaa
    27061 tggtcgcgcc agaaaacctg gaagatcacg taaaactcaa tgagctgaac caagatttag
    27121 aagctgctag acaagatcaa gaaacaaaat tgacggaatg ggaagaacta agcttggaat
    27181 tagaagaata tgaaaattaa cgaaaaaacc aaaacagaaa ccattcatgc tgttttggtt
    27241 tttctttttt tattttatca aacaaaaatt ccatatttac ctttttcacc aataagaaga
    27301 ccaattacta gtcattctca ctaaagcttc taaataatag taatcacccc aaatacaagg
    27361 tgtatttacc ccattatttt tgggcttatt ataaacagac tgtgttaaaa agccatcttc
    27421 ttcttccgtt gtagcatatc tttctttcaa ttgtcgcata attttccaaa cagcagactg
    27481 gtataagttc ttttctgttt tgtctaatag acctgattct attaattcta ataatccaca
    27541 aaccatgatc accatactag atgtatcttt ttcttctctg ctagaatcat caaagatcaa
    27601 atcccaataa caaatggtat cttctggcaa ccgattcata aaataattgg ctaatatttt
    27661 gcttaaatca aaatattttt gttctttcaa ataacgataa cttaatgcaa aaccgtaaat
    27721 cccccatgct tggcctctag cccaacagga attatcacta taaccttgat gcgtacttcc
    27781 atatcttggt tttccatctt tgacatccat ataaaatgta tgataagttg aaccatcttc
    27841 tcttactaaa taatctgctg cattttgaat atgagcctga gcagcttttt tatatttttg
    27901 atttccagtt tcttcactcg cccaatacaa taatggaaga ttcatgcaac aatccatgat
    27961 cattctacct tgttgtgaag gatcactagg atctccccaa gcttgtataa ctcctgcctt
    28021 ctcccaaaaa cgctgaagta atgtttctgc agcatcaatt gaagcttcct tcatttttcg
    28081 atttcccgtt attttataat ctgctacggt tgataaagaa tacaaaaaac caagatcgtg
    28141 atgattagtg ataatttgac agtcaatcct gtggataaaa tctatatctg ctttttcagc
    28201 tgtttgttga aataaaggat cacccgtctt ctcaaataac aaccaattaa ttcctgtcca
    28261 aaatccttct gtccaaccaa tattgtctct taatggataa cgtcctttta taggacactc
    28321 gcttgggaat tgatactgac tgttactgat acgtttgatt aacctctccg tgtgtctgcg
    28381 acaacgctcg atctcattat tccattcttt ttccattaat tctttataac attcatttat
    28441 ccatttttct ggatgtatta tctcttcaaa catcacttta tcaagcattt ccccaccagc
    28501 ctttagcata attttagtct ttttttattg tttttatttt ggatagttag cccggttgtc
    28561 ccatcaaaat caattttggg acgattgttc catacttcta aactataatc ccgatcttga
    28621 tgagcaacca ctcctgttcc gagccaatgg gttccttttc caaatgtacc ctctaatcca
    28681 ggaataaaag tgttttccca tgtcatcaag tttgtattgg gcaaacccat aatagctgtg
    28741 gattttcgtt taatagtatc gtcacttaaa cagataatac ctgaaaagcc agcttcactc
    28801 accctatagt tttccttgtc ttcttctatg attattcctt ctttctcagg agaacattca
    28861 atagcaaacc ctgtctcata tagaacatat tctgtatcta attctacttt gtgtacgcga
    28921 atctgccagc cttcaaaagg aataagccaa gttctgattt ttgcctgtaa agtaggttcc
    28981 catgtagagg caagtacctc tttcttacat acaatgtctc gtacattatt tcgaacgatg
    29041 aactgtccat aggttttgat gctctcattc ttaaaactgg tcgtgatata tgtgtccgct
    29101 cgctgaatcc ctagcattga atccattgcc atttctgctt tcaattgatt cgctcgcggt
    29161 atcgaaaaac caaattgact agaataagca aattttaaat atttttcatt ggtcaatgta
    29221 tggaaatttg gccctaattg tcccgcattc aaaaaaaatg tatgtccatt atcatgttgt
    29281 atgagcaaat tggcttctga aagaagttta gttccttcac gaataggcat aggcatttct
    29341 ttagccgtcc aatactcatg gtcttcaggt agcgccaaca ataaaaaaat cttattcagc
    29401 cataatggag ataacgtact attatagggt tcagtcaatg tcaattgagg gtaggtataa
    29461 ccaatattca ggataccttt ttcatcaaat atttcttttt gcatccacca tcttatattc
    29521 cgattgacta accccttgat ttcacctaat ttaaaaggca aaagatcggc ccacaacata
    29581 gctgaccaaa agcttgaaac tgcaaaacga taaatcatgc ttcgaccaaa aggcacattt
    29641 gcaccatctt ccgcaaaaaa atgaatatag tctctagaaa aatcaatagc tcggttctta
    29701 tatatctggc taatgggctt ttctggttct agtacgctat aaatcaaacc ataataatga
    29761 aatgcaaaag gattataata atcttggcga cctcttgaac tgtctcgata ccagccattt
    29821 tgaagataac aattttctat attatcaaat gcttctttca gtgctgattc tttaactgga
    29881 taaccaaatt tttggataat tgttccgaca attatcttaa aaaattgcca attaccatct
    29941 gcacattcat attcaaaaat ttgcgagaac caatgaataa tctggctttt ttctcctgta
    30001 tctaattttt tccataataa ttgttcatga tgatataacg ctaataacat tgcgggcatt
    30061 tctaccatac gctggtctct ttcatgtatt tctccccaat aatcgggatg ggaaggatct
    30121 gttccagctt ttattcctga tagaattaat ttcaaccatc gttcatctaa ttggtcaatt
    30181 gcgggccccg ctccccataa aacccgagag aatccttcca ttcctgcaat tttatttcca
    30241 tatcctaccc cttgatgtat ataatcaatt tgtgcgccac ttgctcgata aaacggctgg
    30301 agtggttgat ttatcttttt aaataagtca aagtaatcct gtctggtcct taatagtggt
    30361 actggatgtt tcattgtatt ttctcctctt tttactacca aatagctaat tccgactgtt
    30421 ctaatcgaag taagccttca ataaaaaaat aatcagaata gatcagtggc acattataag
    30481 tcagttctct atggtatgct tctgctccgc ctccaatgat cccatcttca tccatattcc
    30541 aatttgcata gtgcttttct gtcttttgga gtatattgat agcagcttca tgatacattt
    30601 ttgatcgtac agaggaaaca tatcctgcta attccagaag accgcatgca gcgcatactc
    30661 cagcactggt atcaatattc gaatctttta cagcaaaatc aatgagagga atataaccag
    30721 ttttttcgat tttctgaata aaatttactg ctatcttttc tgctatagct agatagtacg
    30781 gattttttgt gtaacgataa cttaacgcga atccatataa agcccatgca tttcctctgc
    30841 tccaacaaga attctcattg tatccttgcc cgccaagtaa ccgaatatat ttccctgatt
    30901 ctgcatcaaa ttctgcgata tgcccggttg atccatcttt cctcactaga tacttagctg
    30961 ttgtatctgc atgctgccga gcaattttag agaacctcgg atcaccagtt tccctcgaag
    31021 cccaatacag taatggtaaa ttcatcatac tatcaataat catccaacca ggtttatggt
    31081 cattccatgc tactaaatag ttccctgaaa tattatagcg tccagcaagt accgtagctg
    31141 catgtaaccc tagttgtcga ctatgtttgt tttgagactg ttgataatgt gcaatagccg
    31201 atggtaagaa taaaaaacct acatcatgat gaacgtctga aaaatggtac agggcagatt
    31261 ctaataattg ttcttgttgt attgctgcct ctttgaactt ttctcgtttt gtcttgtgaa
    31321 agagctgcca taaaatacca ctccaaaaac cattggtcca ccagtcgatt cctctgtcct
    31381 ttattacgtc tgtatatttt ccgttgtaag aaatatatgg aattttattc cccattctgt
    31441 ctaattccgc atctatcttt tcagaagcta cattcaaaat ttttcttgac cattccttcg
    31501 tctgttcatt catggatatc ccgccattct tttaattttt atgaatgtgg agttgctcct
    31561 agaacgaact agatggttaa cattcgttat tttttttact tatttgcagc tccaatgtca
    31621 ttctccttaa atttagactg cgcccacaca taaatcaaga ccattgggat cactgaagtt
    31681 gctaaaatta gcataattgc gccataatta attggctcac ctgtactaga aaccgtcata
    31741 tttttgataa ttaagggaac cgtatattta tctttatctg aaagcatgac taatggaaat
    31801 agatattgat tccaacttcc cataaagata agtaaaccac tagtaataat cgtaggtgat
    31861 aaaatcggta cagcgatttt cgtaaaaatc acccactcat tggcaccatc gattctggca
    31921 gcctccatca gtgctgtagg aaattgactg ctatattgtc gcatcataaa cactgaagtt
    31981 gtactggcaa tcgaagggat gatcacacct gcataagtat tcgataatcc taaacgactc
    32041 ataatgtaaa ataaaggaat caatgtggta aaaaaaggca ccatcctaga tagcataact
    32101 gtattgaaca acagtttatt ccccataaaa cgatactttg ctaatgcata acccgccatt
    32161 gtagaactgg ctgtagctaa aactgtactg attacagcaa taacaatcga attccaaata
    32221 atcaagggat aattatatct cgccatgatg gcttgccagt tttttaatag catatcccca
    32281 aacttgaaat tgattccgcc acttaaaatt tcttgattac tttgcgttga agctacaatc
    32341 atatataaat atggcatcaa aaatattaag gcaccaaaca caataatgac gtacgtaaaa
    32401 attctgcttt gtatcttttg tttagtcatt tttatcacct ccgaatttaa attgaatagc
    32461 cgataatata acagatacaa acaccatcgt ccaggcaata gcagaggctt ttccaaaatc
    32521 aaccatttca aaacttgttt tatataaatt gactcctacg accaaagctg aattttctgg
    32581 tccattggaa gtcccaaaga atatatttgg gatattgaaa ttacccaagc cattgatagt
    32641 ggctaaaact gtggccatta aaatgattgg ctttagcatg gggatcgtta ataaaaagaa
    32701 ttgccgaaat ttcgatgctc catctatttc agcactttcg tatatctctt tagaaatccc
    32761 ttgaagacca gctaaaaata aaatagtgtt atatcctaca ctaagccaaa tcatcgtgat
    32821 gatcattgat attcgtatcc aaaaaggatt tgttaaccac tgtacaccgt taattcctag
    32881 cgcattcaaa aatccattga ataacccagt cggattgaaa aaagtcatga atacggaagc
    32941 tacagccacc gttgatgtaa tggttggaat ataatagaca gttctaaaaa atccttttcc
    33001 ttttaactgt gctgaattca ataaaacagc aaagtaaata gctaatagca tcgttattgg
    33061 aattgatcct gccatgataa tcaaaacatt cgtgtaagat ttaataaaca atggatctcg
    33121 gaagatagct agataattgt caaatccaac aaattcaaat gagtagccaa ttttcataaa
    33181 actaaaagca aacgagataa tgatgggaat caaaatcgtc acaataaaga aaataacaaa
    33241 aggtgtgacc aatccataag cacttttttt atgagacgta tctgttgctt ttttcttgct
    33301 gttctttatt gtcttgcttg gtaacgaaga ttgcatcatc gttaagtcct cctaatattt
    33361 tttaatcacc agttaatttt acgccgtact tatgagcaaa attttctgct tcttttagat
    33421 atgaattttc aaaatcacct gtttgccaat agttatatgc agcggtagtc aaataagacg
    33481 tagcatctga ataagtggct ggtaaaacag tatttgatgt attttcattg attttattca
    33541 atagttcata gtatttttgt cctccttagt agtcaaactt tttctcagct gcttctgttt
    33601 gataggccaa tgagttagat gtagccacgc ccagctccag tgcgttggct aagcattctt
    33661 catctgtcag cataaatgta agaaattgtt gtgccacttg tggatttttt gcatctttag
    33721 ggacataaaa acttgatcct cctgatactg caccttttcc tggattttct tttgaaaaag
    33781 gaatcagtgg agccatgcgc catttgtcag atgcatcagg aaaattcaaa gacatattcg
    33841 tagcatacca gccgccagca acaaacattg cagattcttg aaatgctttc tcagcatctt
    33901 gtgcaccgta gaaatgtaca atatctgagt cgatcatttc tttgattaac tttgcagcat
    33961 gtttcgcttc tttagtattc agacggatat ttccttcttc atcaagcaaa gattgattct
    34021 gctgggctag catgttgata aacaattctt ggtccccaga ttctggcaag gcaattccaa
    34081 aactaccagt tttttctttt aatgcttttg ccacctggat atattcttcc caagaactga
    34141 tttcttcaaa atctacacct atttcagcaa atttttttgg ttgataataa caaagtgctg
    34201 gggtaaaatc actagcccaa ggaataatcg tttcattact cgtttgttga cgcaaaatat
    34261 tcaatttttg atcataaaac tgatcgccat atttgtcata aaatccatat tcatcagcac
    34321 tataaaaagc atcttgaaat ttttccagca cacctgctgc tgaagtgtct gccatggaga
    34381 taatatccgg catatcttcg tgtgttacca attttggggt gatcatttgt actgttgctg
    34441 ttcccgaagc tacattcaca atttttaagt caatatttgc tccagttttg cggttataga
    34501 tttttgcagc attttcaaca tagtttgtca gtttgccgtt tgcgacatat acttctaatg
    34561 tagtggcttt ttttatcatt ggatctgcga tgtttgctgt tacttcttcg ttagtttttc
    34621 cacatgcact taagctgaat aatagggtaa tgtttagcaa agctatcacc aatttgtatc
    34681 tttttttcat gtctatccct tctttctttt caagttctta tataaaacgc tttcacttat
    34741 gtatattaac ggaattagta acacaaatat tta
//
LOCUS       DAYYCW010000015        34676 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000015 DAYYCW010000000
VERSION     DAYYCW010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.015"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACHAYA_000739"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACHAYA_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM8604566.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACHAYA_000740"
     CDS             complement(2020..2922)
                     /locus_tag="ACHAYA_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8604567.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACHAYA_000741"
     CDS             complement(2919..3611)
                     /locus_tag="ACHAYA_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM8604568.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACHAYA_000742"
     CDS             complement(3654..4295)
                     /locus_tag="ACHAYA_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM8604569.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="ACHAYA_000743"
     CDS             complement(4333..4647)
                     /locus_tag="ACHAYA_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604570.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHAYA_000744"
     CDS             complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHAYA_000744"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFM8604571.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHAYA_000745"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHAYA_000745"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM8604572.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACHAYA_000746"
     CDS             complement(5984..6916)
                     /locus_tag="ACHAYA_000746"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM8604573.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACHAYA_000747"
     CDS             complement(6913..7689)
                     /locus_tag="ACHAYA_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM8604574.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHAYA_000748"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHAYA_000748"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM8604575.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACHAYA_000749"
     CDS             complement(10933..12009)
                     /locus_tag="ACHAYA_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM8604576.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACHAYA_000750"
     CDS             complement(12014..13294)
                     /locus_tag="ACHAYA_000750"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM8604577.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACHAYA_000751"
     CDS             complement(13313..14239)
                     /locus_tag="ACHAYA_000751"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM8604578.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACHAYA_000752"
     CDS             complement(14252..15535)
                     /locus_tag="ACHAYA_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM8604579.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHAYA_000753"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHAYA_000753"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM8604580.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACHAYA_000754"
     CDS             complement(16323..17234)
                     /locus_tag="ACHAYA_000754"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8604581.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHAYA_000755"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHAYA_000755"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM8604582.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACHAYA_000756"
     CDS             complement(17700..18398)
                     /locus_tag="ACHAYA_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM8604583.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACHAYA_000757"
     CDS             complement(18385..18771)
                     /locus_tag="ACHAYA_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604584.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACHAYA_000758"
     CDS             complement(18918..20588)
                     /locus_tag="ACHAYA_000758"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM8604585.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACHAYA_000759"
     CDS             20771..21004
                     /locus_tag="ACHAYA_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8604586.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACHAYA_000760"
     CDS             complement(21103..21534)
                     /locus_tag="ACHAYA_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM8604587.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="ACHAYA_000761"
     CDS             21613..22026
                     /locus_tag="ACHAYA_000761"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM8604588.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="ACHAYA_000762"
     CDS             complement(22064..23212)
                     /locus_tag="ACHAYA_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8604589.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="ACHAYA_000763"
     CDS             complement(23334..23930)
                     /locus_tag="ACHAYA_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8604590.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHAYA_000764"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHAYA_000764"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM8604591.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="ACHAYA_000765"
     CDS             27160..27591
                     /locus_tag="ACHAYA_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8604592.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="ACHAYA_000766"
     CDS             complement(27636..28985)
                     /locus_tag="ACHAYA_000766"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8604593.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="ACHAYA_000767"
     CDS             complement(29055..30218)
                     /locus_tag="ACHAYA_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8604594.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="ACHAYA_000768"
     CDS             30347..30670
                     /locus_tag="ACHAYA_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604595.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="ACHAYA_000769"
     CDS             complement(30853..32202)
                     /locus_tag="ACHAYA_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8604596.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="ACHAYA_000770"
     CDS             complement(32271..33224)
                     /locus_tag="ACHAYA_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM8604597.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="ACHAYA_000771"
     CDS             complement(33326..34516)
                     /locus_tag="ACHAYA_000771"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM8604598.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9852 a         7595 c         6088 g        11141 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaat
//
LOCUS       DAYYCW010000016        34518 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000016 DAYYCW010000000
VERSION     DAYYCW010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.016"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACHAYA_000772"
     CDS             212..604
                     /locus_tag="ACHAYA_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8604599.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACHAYA_000773"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACHAYA_000773"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM8604600.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACHAYA_000774"
     CDS             2698..2943
                     /locus_tag="ACHAYA_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604601.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACHAYA_000775"
     CDS             3165..4775
                     /locus_tag="ACHAYA_000775"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM8604602.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACHAYA_000776"
     CDS             complement(4752..4964)
                     /locus_tag="ACHAYA_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604603.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACHAYA_000777"
     CDS             5067..5936
                     /locus_tag="ACHAYA_000777"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM8604604.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACHAYA_000778"
     CDS             6101..7372
                     /locus_tag="ACHAYA_000778"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8604605.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACHAYA_000779"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACHAYA_000779"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM8604606.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACHAYA_000780"
     CDS             8763..9026
                     /locus_tag="ACHAYA_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM8604607.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACHAYA_000781"
     CDS             9471..9920
                     /locus_tag="ACHAYA_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8604608.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACHAYA_000782"
     CDS             9977..11395
                     /locus_tag="ACHAYA_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8604609.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACHAYA_000783"
     CDS             11395..12255
                     /locus_tag="ACHAYA_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8604610.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACHAYA_000784"
     CDS             12429..13202
                     /locus_tag="ACHAYA_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8604611.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACHAYA_000785"
     CDS             13349..13714
                     /locus_tag="ACHAYA_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM8604612.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACHAYA_000786"
     CDS             complement(13862..15070)
                     /locus_tag="ACHAYA_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8604613.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACHAYA_000787"
     CDS             complement(15089..15367)
                     /locus_tag="ACHAYA_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604614.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACHAYA_000788"
     CDS             15560..16216
                     /locus_tag="ACHAYA_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8604615.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACHAYA_000789"
     CDS             complement(16658..17455)
                     /locus_tag="ACHAYA_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM8604616.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACHAYA_000790"
     CDS             complement(17452..18636)
                     /locus_tag="ACHAYA_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM8604617.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACHAYA_000791"
     CDS             complement(18661..19908)
                     /locus_tag="ACHAYA_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8604618.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHAYA_000792"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHAYA_000792"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM8604619.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACHAYA_000793"
     CDS             21888..22202
                     /locus_tag="ACHAYA_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM8604620.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACHAYA_000794"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACHAYA_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM8604621.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACHAYA_000795"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACHAYA_000795"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM8604622.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACHAYA_000796"
     CDS             23546..23875
                     /locus_tag="ACHAYA_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM8604623.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACHAYA_000797"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACHAYA_000797"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM8604624.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACHAYA_000798"
     CDS             24863..25687
                     /locus_tag="ACHAYA_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM8604625.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACHAYA_000799"
     CDS             25680..26027
                     /locus_tag="ACHAYA_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM8604626.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHAYA_000800"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHAYA_000800"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM8604627.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACHAYA_000801"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACHAYA_000801"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM8604628.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHAYA_000802"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHAYA_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM8604629.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHAYA_000803"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHAYA_000803"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM8604630.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACHAYA_000804"
     CDS             31684..32451
                     /locus_tag="ACHAYA_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8604631.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACHAYA_000805"
     CDS             32441..34483
                     /locus_tag="ACHAYA_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8604632.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11100 a         6121 c         7249 g        10048 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaa
//
LOCUS       DAYYCW010000017        34474 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000017 DAYYCW010000000
VERSION     DAYYCW010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.017"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHAYA_000806"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHAYA_000806"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM8604633.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHAYA_000807"
     CDS             complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHAYA_000807"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM8604634.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHAYA_000808"
     CDS             complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHAYA_000808"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM8604635.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="ACHAYA_000809"
     CDS             2179..2499
                     /locus_tag="ACHAYA_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM8604636.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3468)
                     /locus_tag="ACHAYA_000810"
     CDS             complement(2611..3468)
                     /locus_tag="ACHAYA_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8604637.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(3576..4880)
                     /locus_tag="ACHAYA_000811"
     CDS             complement(3576..4880)
                     /locus_tag="ACHAYA_000811"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8604638.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4877..5386)
                     /locus_tag="ACHAYA_000812"
     CDS             complement(4877..5386)
                     /locus_tag="ACHAYA_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604639.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5561..7696)
                     /locus_tag="ACHAYA_000813"
     CDS             complement(5561..7696)
                     /locus_tag="ACHAYA_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM8604640.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7779..9434)
                     /locus_tag="ACHAYA_000814"
     CDS             complement(7779..9434)
                     /locus_tag="ACHAYA_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8604641.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9436..9750)
                     /locus_tag="ACHAYA_000815"
     CDS             complement(9436..9750)
                     /locus_tag="ACHAYA_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8604642.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9920..10180
                     /locus_tag="ACHAYA_000816"
     CDS             9920..10180
                     /locus_tag="ACHAYA_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM8604643.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10194..10508
                     /locus_tag="ACHAYA_000817"
     CDS             10194..10508
                     /locus_tag="ACHAYA_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM8604644.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11238..11792)
                     /locus_tag="ACHAYA_000818"
     CDS             complement(11238..11792)
                     /locus_tag="ACHAYA_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM8604645.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11918..13847)
                     /locus_tag="ACHAYA_000819"
                     /pseudo
     CDS             complement(11918..13847)
                     /locus_tag="ACHAYA_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14153..14869)
                     /locus_tag="ACHAYA_000820"
     CDS             complement(14153..14869)
                     /locus_tag="ACHAYA_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8604646.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHAYA_000821"
     CDS             complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHAYA_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM8604647.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHAYA_000822"
     CDS             complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHAYA_000822"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM8604648.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18339..19799)
                     /locus_tag="ACHAYA_000823"
     CDS             complement(18339..19799)
                     /locus_tag="ACHAYA_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8604649.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19789..21084)
                     /locus_tag="ACHAYA_000824"
     CDS             complement(19789..21084)
                     /locus_tag="ACHAYA_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8604650.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21416..22093)
                     /locus_tag="ACHAYA_000825"
     CDS             complement(21416..22093)
                     /locus_tag="ACHAYA_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM8604651.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22123..23214)
                     /locus_tag="ACHAYA_000826"
     CDS             complement(22123..23214)
                     /locus_tag="ACHAYA_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM8604652.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23315..23749)
                     /locus_tag="ACHAYA_000827"
     CDS             complement(23315..23749)
                     /locus_tag="ACHAYA_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8604653.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24333..25790)
                     /locus_tag="ACHAYA_000828"
     CDS             complement(24333..25790)
                     /locus_tag="ACHAYA_000828"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8604654.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25787..27682)
                     /locus_tag="ACHAYA_000829"
     CDS             complement(25787..27682)
                     /locus_tag="ACHAYA_000829"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8604655.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27740..29515)
                     /locus_tag="ACHAYA_000830"
     CDS             complement(27740..29515)
                     /locus_tag="ACHAYA_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8604656.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29565..31061)
                     /locus_tag="ACHAYA_000831"
     CDS             complement(29565..31061)
                     /locus_tag="ACHAYA_000831"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8604657.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31255..32877)
                     /locus_tag="ACHAYA_000832"
     CDS             complement(31255..32877)
                     /locus_tag="ACHAYA_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8604658.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33205..>34474)
                     /locus_tag="ACHAYA_000833"
     CDS             complement(33205..>34474)
                     /locus_tag="ACHAYA_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8604659.1"
                     /translation="TTVKREVLAGFTTFISMAYILFVNPTVLGASGMDEGAVFTATAL
                     ASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWETALAGVFVASLIFILI
                     TIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSNESTLVGLGSLNVGST
                     WLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPSEIVAAAPSLKPTFLV
                     ALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMKDNKMPRVGKALASDS
                     TAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLLGLFFSPLLSVVTSQV
                     TAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSISDGIALGFIFYPITM
                     LAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
BASE COUNT         9777 a         7458 c         5830 g        11409 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
     4861 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
     4921 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
     4981 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
     5041 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
     5101 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
     5161 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
     5221 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
     5281 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
     5341 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
     5401 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
     5461 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
     5521 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
     5581 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
     5641 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
     5701 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
     5761 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
     5821 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
     5881 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
     5941 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
     6001 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
     6061 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
     6121 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
     6181 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
     6241 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
     6301 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
     6361 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
     6421 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
     6481 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
     6541 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
     6601 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
     6661 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
     6721 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
     6781 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
     6841 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
     6901 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
     6961 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
     7021 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
     7081 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
     7141 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
     7201 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
     7261 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
     7321 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
     7381 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
     7441 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
     7501 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
     7561 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
     7621 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
     7681 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
     7741 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
     7801 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
     7861 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
     7921 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
     7981 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
     8041 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
     8101 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
     8161 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
     8221 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
     8281 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
     8341 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
     8401 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
     8461 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
     8521 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
     8581 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
     8641 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
     8701 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
     8761 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
     8821 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
     8881 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
     8941 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
     9001 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
     9061 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
     9121 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
     9181 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
     9241 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
     9301 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
     9361 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
     9421 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
     9481 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
     9541 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
     9601 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
     9661 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
     9721 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
     9781 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
     9841 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
     9901 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
     9961 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    10021 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    10081 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    10141 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    10201 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    10261 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    10321 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    10381 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    10441 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    10501 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    10561 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    10621 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    10681 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    10741 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    10801 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    10861 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    10921 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    10981 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    11041 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    11101 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    11161 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    11221 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    11281 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    11341 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    11401 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    11461 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    11521 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    11581 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    11641 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    11701 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    11761 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    11821 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    11881 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    11941 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    12001 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    12061 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    12121 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    12181 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    12241 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    12301 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    12361 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    12421 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    12481 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    12541 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    12601 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    12661 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    12721 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    12781 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    12841 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    12901 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    12961 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    13021 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    13081 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    13141 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    13201 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    13261 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    13321 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    13381 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    13441 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    13501 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    13561 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    13621 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    13681 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    13741 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    13801 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    13861 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    13921 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    13981 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    14041 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    14101 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    14161 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    14221 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    14281 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    14341 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    14401 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    14461 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    14521 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    14581 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    14641 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    14701 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    14761 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    14821 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    14881 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    14941 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    15001 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    15061 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    15121 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    15181 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    15241 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    15301 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    15361 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    15421 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    15481 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    15541 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    15601 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    15661 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    15721 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    15781 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    15841 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    15901 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    15961 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    16021 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    16081 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    16141 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    16201 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    16261 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    16321 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    16381 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    16441 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    16501 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    16561 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    16621 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    16681 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    16741 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    16801 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    16861 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    16921 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    16981 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    17041 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    17101 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    17161 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    17221 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    17281 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    17341 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    17401 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    17461 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    17521 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    17581 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    17641 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    17701 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    17761 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    17821 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    17881 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    17941 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    18001 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    18061 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    18121 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    18181 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    18241 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    18301 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    18361 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    18421 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    18481 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    18541 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    18601 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    18661 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    18721 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    18781 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    18841 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    18901 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    18961 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    19021 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    19081 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    19141 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    19201 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    19261 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    19321 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    19381 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    19441 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    19501 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    19561 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    19621 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    19681 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    19741 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    19801 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    19861 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    19921 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    19981 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    20041 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    20101 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    20161 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    20221 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    20281 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    20341 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    20401 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    20461 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    20521 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    20581 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    20641 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    20701 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    20761 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    20821 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    20881 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    20941 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    21001 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    21061 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    21121 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    21181 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    21241 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    21301 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    21361 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    21421 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    21481 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    21541 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    21601 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    21661 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    21721 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    21781 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    21841 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    21901 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    21961 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    22021 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    22081 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    22141 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    22201 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    22261 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    22321 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    22381 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    22441 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    22501 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    22561 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    22621 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    22681 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    22741 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    22801 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    22861 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    22921 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    22981 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    23041 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    23101 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    23161 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    23221 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    23281 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    23341 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    23401 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    23461 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    23521 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    23581 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    23641 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    23701 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    23761 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    23821 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    23881 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    23941 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    24001 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    24061 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    24121 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    24181 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    24241 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    24301 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    24361 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    24421 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    24481 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    24541 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    24601 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    24661 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    24721 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    24781 accccttccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    24841 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    24901 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    24961 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    25021 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    25081 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    25141 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    25201 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    25261 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    25321 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    25381 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    25441 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    25501 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    25561 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    25621 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    25681 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    25741 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    25801 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    25861 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    25921 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    25981 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    26041 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    26101 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    26161 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    26221 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    26281 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    26341 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    26401 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    26461 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    26521 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    26581 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    26641 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    26701 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    26761 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    26821 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    26881 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    26941 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    27001 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    27061 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    27121 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    27181 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    27241 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    27301 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    27361 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    27421 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    27481 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    27541 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    27601 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    27661 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    27721 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    27781 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    27841 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    27901 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    27961 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    28021 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    28081 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    28141 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    28201 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    28261 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    28321 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    28381 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    28441 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    28501 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    28561 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    28621 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    28681 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    28741 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    28801 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    28861 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    28921 gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg
    28981 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    29041 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    29101 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    29161 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    29221 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    29281 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    29341 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    29401 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    29461 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    29521 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    29581 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    29641 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    29701 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    29761 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    29821 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    29881 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    29941 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    30001 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    30061 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    30121 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    30181 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    30241 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    30301 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    30361 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    30421 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    30481 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    30541 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    30601 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    30661 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    30721 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    30781 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    30841 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    30901 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    30961 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    31021 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    31081 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    31141 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    31201 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    31261 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    31321 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
    31381 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    31441 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    31501 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    31561 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    31621 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    31681 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    31741 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    31801 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    31861 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    31921 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    31981 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    32041 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    32101 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    32161 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    32221 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    32281 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    32341 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    32401 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    32461 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    32521 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    32581 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    32641 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    32701 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    32761 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    32821 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    32881 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    32941 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    33001 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
    33061 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
    33121 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
    33181 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
    33241 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
    33301 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
    33361 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
    33421 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
    33481 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
    33541 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
    33601 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
    33661 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
    33721 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
    33781 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
    33841 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
    33901 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
    33961 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
    34021 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
    34081 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
    34141 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
    34201 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
    34261 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
    34321 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
    34381 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
    34441 aaatcctgcc agtacctcac gttttaccgt ggtg
//
LOCUS       DAYYCW010000018        32409 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000018 DAYYCW010000000
VERSION     DAYYCW010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.018"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..199
                     /locus_tag="ACHAYA_000834"
     CDS             <1..199
                     /locus_tag="ACHAYA_000834"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFM8604660.1"
                     /translation="RDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILS
                     DIRWVLRENQRPHYCIMRKIL"
     gene            687..1652
                     /locus_tag="ACHAYA_000835"
     CDS             687..1652
                     /locus_tag="ACHAYA_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFM8604661.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            1674..1886
                     /locus_tag="ACHAYA_000836"
     CDS             1674..1886
                     /locus_tag="ACHAYA_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8604662.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            1961..2437
                     /locus_tag="ACHAYA_000837"
     CDS             1961..2437
                     /locus_tag="ACHAYA_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFM8604663.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            2434..4242
                     /locus_tag="ACHAYA_000838"
     CDS             2434..4242
                     /locus_tag="ACHAYA_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8604664.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            4319..4534
                     /locus_tag="ACHAYA_000839"
     CDS             4319..4534
                     /locus_tag="ACHAYA_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFM8604665.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            4626..5489
                     /locus_tag="ACHAYA_000840"
     CDS             4626..5489
                     /locus_tag="ACHAYA_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFM8604666.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            5526..6062
                     /locus_tag="ACHAYA_000841"
     CDS             5526..6062
                     /locus_tag="ACHAYA_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFM8604667.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            6076..6498
                     /locus_tag="ACHAYA_000842"
     CDS             6076..6498
                     /locus_tag="ACHAYA_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM8604668.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            6428..8827
                     /locus_tag="ACHAYA_000843"
     CDS             6428..8827
                     /locus_tag="ACHAYA_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM8604669.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            8859..10850
                     /locus_tag="ACHAYA_000844"
     CDS             8859..10850
                     /locus_tag="ACHAYA_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM8604670.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            10873..11124
                     /locus_tag="ACHAYA_000845"
     CDS             10873..11124
                     /locus_tag="ACHAYA_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM8604671.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            11114..12343
                     /locus_tag="ACHAYA_000846"
     CDS             11114..12343
                     /locus_tag="ACHAYA_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM8604672.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            12340..14421
                     /locus_tag="ACHAYA_000847"
     CDS             12340..14421
                     /locus_tag="ACHAYA_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM8604673.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            14570..18490
                     /locus_tag="ACHAYA_000848"
     CDS             14570..18490
                     /locus_tag="ACHAYA_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM8604674.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            18491..19435
                     /locus_tag="ACHAYA_000849"
     CDS             18491..19435
                     /locus_tag="ACHAYA_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604675.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            19439..19642
                     /locus_tag="ACHAYA_000850"
     CDS             19439..19642
                     /locus_tag="ACHAYA_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM8604676.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(19942..20178)
                     /locus_tag="ACHAYA_000851"
     CDS             complement(19942..20178)
                     /locus_tag="ACHAYA_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8604677.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(20361..20732)
                     /locus_tag="ACHAYA_000852"
     CDS             complement(20361..20732)
                     /locus_tag="ACHAYA_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM8604678.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(20729..22057)
                     /locus_tag="ACHAYA_000853"
     CDS             complement(20729..22057)
                     /locus_tag="ACHAYA_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8604679.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(22018..22347)
                     /gene="mobC"
                     /locus_tag="ACHAYA_000854"
     CDS             complement(22018..22347)
                     /gene="mobC"
                     /locus_tag="ACHAYA_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8604680.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(22605..22975)
                     /locus_tag="ACHAYA_000855"
                     /pseudo
     CDS             complement(22605..22975)
                     /locus_tag="ACHAYA_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            23120..23305
                     /locus_tag="ACHAYA_000856"
     CDS             23120..23305
                     /locus_tag="ACHAYA_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8604681.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            23463..23630
                     /locus_tag="ACHAYA_000857"
     CDS             23463..23630
                     /locus_tag="ACHAYA_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604682.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            24248..24907
                     /gene="vanR-B"
                     /locus_tag="ACHAYA_000858"
     CDS             24248..24907
                     /gene="vanR-B"
                     /locus_tag="ACHAYA_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8604683.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            24910..26271
                     /gene="vanS-B"
                     /locus_tag="ACHAYA_000859"
     CDS             24910..26271
                     /gene="vanS-B"
                     /locus_tag="ACHAYA_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8604684.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(26362..27663)
                     /locus_tag="ACHAYA_000860"
     CDS             complement(26362..27663)
                     /locus_tag="ACHAYA_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8604685.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            27942..28748
                     /locus_tag="ACHAYA_000861"
     CDS             27942..28748
                     /locus_tag="ACHAYA_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM8604686.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            28766..29473
                     /locus_tag="ACHAYA_000862"
     CDS             28766..29473
                     /locus_tag="ACHAYA_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM8604687.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            29470..30441
                     /locus_tag="ACHAYA_000863"
     CDS             29470..30441
                     /locus_tag="ACHAYA_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8604688.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            30434..31462
                     /gene="vanB"
                     /locus_tag="ACHAYA_000864"
     CDS             30434..31462
                     /gene="vanB"
                     /locus_tag="ACHAYA_000864"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8604689.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            31468..32076
                     /locus_tag="ACHAYA_000865"
     CDS             31468..32076
                     /locus_tag="ACHAYA_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8604690.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(32142..32315)
                     /locus_tag="ACHAYA_000866"
     CDS             complement(32142..32315)
                     /locus_tag="ACHAYA_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604691.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         8242 a         8215 c         8721 g         7231 t
ORIGIN      
        1 aagagatgat tatgaaaaat atgatgattc gtacgcgtta tttaaagaaa ctttaaaagg
       61 agatttgtta acaaaaactt ttctcttttt aggatttagt tttacagacc ctaatttaga
      121 aagaatttta tcagatataa gatgggtttt gagagaaaac caaagacctc attattgtat
      181 aatgagaaaa atattataga aaaaatttta ggttatcgct gaaagccccg gaaatacggg
      241 catttcgcag gatatgagaa ctcaatttac taccaattta ctactttcgg attgagccgc
      301 atatatgctg aaccatactg ctaatacccg ccaccccaaa tacggcacat cataaattga
      361 atacgacacc gtaaacgcgg cgttcctctt tcccgtcatg ggagaacagg acgccgcttt
      421 tttatgccca aatagagaaa ggaggcgacg ccatgctatt tacttccggc cctgaccggg
      481 cgtttgaacg gctgatgcag gaaaagcccg gtttcgatca ctacgacggc ggcggggcgg
      541 acgcgccgga ggattgcggc acctgccgat tttaccgccc ggattggaaa tatcaattct
      601 gccagtttgc agagtgtccc tatcagccgg gcaagctgac cgactatgca gcggtcaaat
      661 tcacagtgaa aggagcgaac aacgctatgg cagttttccg tgtggaaaag aacaaaggct
      721 atacggtgat gtcaaatcac cacctgcgga ataagaacct gaccttaaag gccaagggcc
      781 tgctgtcgca gatgttgtcc ttgccggagg attgggacta taccctaaaa ggactgtcct
      841 atatcaaccg ggaaagcatc gacgcgatca ggacagcggt atgggagctt gaaaaagctg
      901 gatatatccg gcgtgagcag ggccgtgacg caaaaggcaa aatggccgat atggtctata
      961 ccatctacga acagccggta ttggaaaacc cggtattgga aaatccaaca tcggataacc
     1021 cggtgttgga aaatccgaca tcggataatc cggcgtcgga aaatccaacg caattaaata
     1081 aagaagtaca aagaactgac ttatcaaaaa aagaaaaaat aattacagat ggacaaagta
     1141 cccattccat tcctatccca tccccgaccc ctgcccctat ggagggcgag gcagcggcta
     1201 cgccgccgga acggaaaagg acaggaaagg atgaggctgt acggatttat cgggaaatca
     1261 ttttggagaa catcgagtat gcctatctca ttcaggacag gagcattgac cgtgaacagc
     1321 ttgacgaaat cgtagacctg atgctggaaa ccgtctgcac cgcccgaaag acaatccgtg
     1381 tcgccgggga cgactaccct gccgagctgg tgaaatccaa gtacatgaag ctgaccggcg
     1441 aacatatccg ctttgtcctt gactgtctgc gggagaacac aacgaaagtc cgcaatatca
     1501 aacaatacct gcgggctatg ctgttcaacg cccccagcac catttccaac tactacacgg
     1561 cccttgtcgc gcacgacatg gcacagcccg attggggaaa gcctaaatcc ggcctgccgg
     1621 actattcctg cggcgagggc gaaagcctgt aacgagaaaa ggaggacgac cctatgaagc
     1681 agggcgcatt gatttttgac gagcgaacag accgttacga catccgcttt gatctggccg
     1741 actattacgg cggcctgcat tgcggggagt gtttcgacgt gatgatcggc ggcaaatgga
     1801 ggcccacccg cattgaaatg gccgaaaact ggtatcttgt cggcatccgg gccgacgacc
     1861 tttccggcct gcgggtgcgg atttgaccta tatctacctg cggcgtctgg tggttccgcc
     1921 ttagacgccg cttttgactt tctgacaagg agggatgcga ttgcaggaag aagttactca
     1981 aaagaccatt gccctgtcca tgaaaacggg caagctcacg gctcaagtgc tgcaagcggc
     2041 gctgaaaaag tacctgcaac accgggccaa gggcaaaaac acgctgcacc acggccagca
     2101 gagcctaaag cagcttaaaa agcatggggc tgatctctcc aacattgaga tcaccgaggc
     2161 caacattgga gcgttcaagc catgcgccaa gaaatacggc gtggatttta ccttacgaaa
     2221 agataagacc acccagccgc cccactacgt tgtgattttc aaggccaagg atgcagataa
     2281 ccttgatctg gcgtttcggg agtttaccgc caagacgctt tccaaggagc agcggccctc
     2341 tatccgcaag gttttgtccg ctgtgaagca aaaggcggcg gcccagacca agcagcgggc
     2401 caaggagaaa atcaaggaaa gggggctgga actgtgaagc ctgacgtaaa gaaattgctg
     2461 atcctgaacc tcccgtatct gatttttgtg tacctgtttg cgaaatgcgg ggaggcgtac
     2521 cggctggccg ctggtgctga cctgtcgggg aagctcctgc attttgcgga tggttttgcg
     2581 gcggcttttg ccaaccctct gccaagcctg cacctgtttg acctgtgtat cggtatcgcc
     2641 ggtgccgtga tcgtgcggct ggtggtgtac tgcaagagca agaacgtgaa gaaataccgc
     2701 aaaggcatgg agtatggcag cgcccgctgg gggacggcca aagacatagc gccttacatc
     2761 gaccccaagt ttgaaaataa tatcctgctc acccaaacgg aacggcttac catgaccggc
     2821 aggcccaaag accccaagac agcgcggaat aaaaacgtgc tggtgatcgg tggcagcggt
     2881 tcagggaaaa cgagattttt cgtgaaaccc aacctgatgc aatgctttcc gaccacggac
     2941 tatccgactt cgtttgtggt gacagatccg aaaggcactt tggttctgga aaccggcagg
     3001 atgttccagc aggcgggcta ccgcattaaa atcctgaata ccattaactt ttccaagtcc
     3061 atgaagtaca acccctttgt ctacatccat tcggaaaagg acgtgctaaa gctggtaaat
     3121 accctgattt ccaacaccaa gggcgagggg gaaaagtcag cggaggattt ttgggtgaag
     3181 tcggaacggt tgttttattc ggccctgatc ggctatatct ggtacgaggc cccagcggag
     3241 gaaatgaact tctcaacgct gctggaaatg ataaacgcca gtgaagcccg cgaggacgac
     3301 ccggagtttc aaagccccgt cgatctcatg tttgagcgtt tggagcagaa agaccccgac
     3361 cattttgcgg tgcgccagta caagaaattc ctgctgtccg caggcaagac ccgttcttcc
     3421 atcctgattt catgcggtgc cagacttgcg ccctttgaca tccgggaggt acgcgagctg
     3481 atggaggacg acgaaatgga gctggacacc atcggggacg aaaagacggt gctgtatctc
     3541 attatgagcg atacggacac gacctttaat ttcattctgg ctatgctcca aagccagcta
     3601 atcaacctgc tttgtgaccg ggctgatgat gtgtacggcg ggcggctgcc tgtccatgta
     3661 cgcctgattt tggatgaatt tgccaacatt ggccaaatac ccaatttcga taaactcatt
     3721 gccaccattc gaagccgtga aatatcggcc tctattattt tgcagagcca gtcgcagcta
     3781 aaagcgattt acaaggacgc cgccgagatc atttcggata actgcgatag tgtgcttttc
     3841 cttagtgggc gcggcaaaaa tgccaaggag atttccgatg cgctggggaa agaaacgatt
     3901 gacagtttca acaccagcga gaaccgggga agccaaacgt cccacggatt gaactatcaa
     3961 aaattaggaa aggagttgat gtcccaagac gaaattgccg tgatggacgg cggcaagtgt
     4021 atcttgcagc tacggggtgt gcgcccgttt ttatcggaga aattcgatat taccaagcat
     4081 ccccggtaca aataccttgc cgacgccgac aagaagaata cctttgacgt ggacagattt
     4141 ttgactatca cgcgccggaa acggcagcaa gtggtaacgc aggatgaagt attcgacctg
     4201 tatgaaatcg acctgtcgga cgaggacgcc gccgctgaat aaacagcggc ttttttcatt
     4261 ccaacaacaa aatcaaaaaa tcattatgag ccggaaaccg gcagaaagag gtaaatttat
     4321 ggcatttttt aattccgcag ttactacttt gcagactatc gtgatcggac tgggcggcgc
     4381 tctgtgcgta tggggcggca tcaatctgtt ggagggatac gggcaggaca atccgggggc
     4441 aaaatcgcag ggtgtcaagc agcttgtcgc tggcggcggc gtggccctga tcggtgtcac
     4501 tctggttccc ctgctgtccg ggctgctggg ctgatcctgg cctttgtccc tgtccaaacc
     4561 cttaactaat cgcctccgcg tcctcccggc cagaccggga gggcgcggga aaggaggtgc
     4621 tacccgtgat tagtgaaatc attgaagaat ggataaaagg catcctcatg gacggtatca
     4681 cgggcaacct gtccgggctg ttcgatacgg taaatgccaa agtcggagaa atcgccgccg
     4741 acgtgggacg aaccccgcag gcatggaaca gcggtatttt caatatgctg cacagcctgt
     4801 cggaaaccgt gatcgtcccc atagccgggg ccatactcgc ccttgtcatg tgctatgagc
     4861 ttatccagat gataacggaa aagaacaata tgcacgactt tgacagctcc atgttcttcc
     4921 gctggatatt caaatccgcg tttgcaatcc tgatcgtaac gaacacatgg aacatcgtca
     4981 tgggcgtatt cgacgccacc caaagcgtcg tcaatcaaag cgccggtgtg attatcgggc
     5041 agacatccat tgattttaac accctgatac cagaccttga aagccaattg gaggctatga
     5101 gcgtgggcgc tcttttgggg ctgtggttcc aaacccttgt tgtgggcctc accatgaata
     5161 tcctttccat ctgcattttc cttgtgacct atggccgcat ggtggaaatc tatgttgtca
     5221 cgtcgctggg gcctatcccc cttgccacaa tgggcaacgc ggaatggcgc ggcatgggcc
     5281 aagggtatct gaaatccctg ctggcgctgg gcttccaagc ctttttaatc atggttgtgg
     5341 tgggtatcta cgcggtgctg atacagaata tcggttccgc ttccgacata tccggcgcaa
     5401 tctggggctg tatgggctat acggtgctgc tgtgcttctg cctgttcaag accggcagta
     5461 tatcaaaggc cgtattcggg gcgcattgat cccaaaatcc aagcatacac aggaggaact
     5521 tgtttatttc aacgtacaac atcatttacg ctgacccgcc gtggcggtat gactgcaaaa
     5581 acgtacaggg cgcagcggaa aatcactatt ccaccatgag cattgacgag ctgtgcgccc
     5641 tgccggtgga acggctggcg tccaaagact gcctgctgtt tttgtgggcc acattcccca
     5701 tgctgccgga agctctgcgg ttaatcaaag cgtggggctt ttcattcaag accgtggctt
     5761 ttgtatggct gaaacagaac cgtaaaagcc ttacatggtt ttacgggctg ggccgctgga
     5821 caaggggaaa cgcggaaatc tgcctgctgg caacacacgg caagccgaag cgtcgttctg
     5881 cctctgtcca tcagtttatt atcagcccga ttgaacaaca cagcaaaaag ccggatgtga
     5941 cccgtgaaaa aatcgtagag ctggcgggcg atttgccccg cgtggagctg tttgccagac
     6001 agaaaacccc cggctgggac gtgtggggga acgaggtgga atgtgacctt atcctgacgt
     6061 gaaaggaggt gccaaatggc ttatgtaacc gtccccaagg acttaaccca tgtgaaaagc
     6121 aaggttgtgt ttgggcttac caaacggcaa ctgatctgtt tttccggggc gctggtgatc
     6181 ggcgtcccct tatattttct cacccgtgac tatcttacca acagcgcggc ggccctgctc
     6241 atggtatttg ccatgctgcc gggctttttg cttgccatgt acgagcgtca cggccagccc
     6301 cttgaagtgg tgattatgca gatcatccag tgctgcttca tccagccaaa ggagcgtccc
     6361 tatcagaccg acaacaccta caccgccctt gtgcggcaat accagatgga acaggaggta
     6421 aaggccattg tccaaaaagg aaatcccaag agaaaccgaa aaaacaaagc tgacccgcgc
     6481 ccagcgaaaa gaaattgacg cggtaatccg taaatataag ggcgacggca ggccccacac
     6541 ggcgcagcag agcatcccct acgaggtcat gtacccggac ggcgtgtgcc gggtatcgcc
     6601 cggcgtcttt tccaagtgca ttgagtttgc ggacatcagc tatcagcttg cccagccgga
     6661 cacgcagacg gctatctttg aaaaactgtg cgacctgtat aactatgtgg acgcctccat
     6721 tcatatccag ttttctttcc tcaatcggaa agttgacccg gtgcagtacg ccaagagctt
     6781 tgagatcgcg ccgcaggggg acgacttcga cgacatccgg gccgagtaca ccggcattct
     6841 gcaaaagcag cttgcaaacg gcaacaacgg catggtaaag accaaatacc tgacattcac
     6901 catcgaggcc gaaagcgtga aagcggcgcg agccagattg aagcgcatcg gctttgacct
     6961 gttaggctat ttcaagagca tgggggccgt agcccatgtg atggacggct gggaacggct
     7021 gaacctgctg catggcgtgt accacccgga cggcgagata ttcaattttg actggaagtg
     7081 gctggccccg tccggcctct ccaccaagga ttttattgcc ccgtcctccc tgtgcttcgg
     7141 caatgcaaag acattcggca tgggcggcaa atacggcgcg gtaagtttcc tgcaaatcct
     7201 gtccccggag ctgtcggacg atatgctggc cgacttcctc aatacggaaa gcggtgtttt
     7261 agtcaatctc catgtgcagg ccattgaaca gaccaaggcg atcaagacca tcaagcgcaa
     7321 aattaccgac cttgacgcta tgaagattgc ggagcagaaa aaggccgtcc gcagcggtta
     7381 cgatatggac attcttccca gcgaccttgc cacctacggc gaggacgcca agaagctgct
     7441 gacaaagttg cagacccgca atgaacggct gttccagttg acgttccttg tcctcaacgt
     7501 ggccgacacc aagcaaaagc tgaataacga cgtgttccaa gccgcaggcg tggcccaaaa
     7561 gcataactgt ccccttgtcc ggctggacta ccagcaggaa cagggccttg caagcagcct
     7621 gccgctgggc gtcaatcaga tcaagataca gcgcagcctt acgacttcca gcgtggccgt
     7681 gttcgtcccc tttgtgacgc aggagctttt tcagggcggc gcggccatgt attacggcat
     7741 caacgccaag tcccgcaaca tgattatgct ggacaggaag caggcccgtt gccccaatgc
     7801 cttgaagctc ggcacccccg gcagcggcaa atccatgagc tgtaaatccg agatcgtcag
     7861 tgtgttctta acgacgcctg acgatatttt tatttcagac ccggaggccg aatattaccc
     7921 ccttgtcaaa cggctccacg gacaggtaat ccggctgtcg cctaccagca aggactttgt
     7981 gaacccgctg gacatcaacc tgaattattc ggaggacgat aacccgctgg ccctgaaatc
     8041 cgactttgtg ctgtcgttct gtgagcttgt catgggcggc aagaacgggc tggaagccat
     8101 tgaaaagacc gtgatagacc gggctgtgcg cgtgatctac cggccctatc tggcagaccc
     8161 ccgcccggag aatatgccga tcctgtccga cctccacaaa gcgttgttag atcagcatgt
     8221 cccggaggct gaccgggtgg cgcaggccct tgacctgtat gtgtccggct ccctgaacgt
     8281 atttaaccac cgtaccaacg tggacatcgg caaccggctt gtgtcctttg acattaagga
     8341 gctgggcaag cagctcaaaa agctggggat gctcattgtc caagaccaga tatgggggcg
     8401 cgtcacggcc aaccgaagcc aaggaaaggc cacatggtat tttgccgacg agttccattt
     8461 gcttttgaaa gaggaacaga cggcggccta ttctgcggag atttggaagc ggtttaggaa
     8521 atggggcggc attccaacag gtgccaccca gaacgtcaaa gacctgcttt cctccccgga
     8581 aattgaaaat atccttgaaa acagcgactt cattacgctg ctcaatcagg cgtccgggga
     8641 tagaaaaatc cttgcggaac gcttgaattt gtccaccgaa cagcagaagt acatcgacaa
     8701 ttccgaaccc ggcgagggcc ttttgatctt tgaaaacgtg gtgctgccct ttaccaaccc
     8761 catcccgcac aacacccagc tttacaagat tatgacgacc cgcttaaacg aggtggcggg
     8821 agtatgaccc ggcgacgcca caaaggaggt gaaccaccat gaaagagctg aaagccaagg
     8881 cgaaagtgac gcaggccatg acccgcgacg ggctgacgct ggaaaatcag gccaccggcg
     8941 aggtggagaa catttccagc cgggaggccg aacaggattt gtccgcgccg gggccgggcg
     9001 gcaccgccga taagctgctg gaacgggccg acgccatcca tgacggccat aaggccaaga
     9061 aagccgcgaa gcaggccggg gctgtggccg ccgaggggga cgctgccctg caccggccat
     9121 ccgcccggct ccaactgtcg gaggaagaac gggccgaccc ggatttacag ccctatatcc
     9181 gaaaatcgga caaacgggcc gataagctgg ataaggcccg cgccgccatc cccaagaagc
     9241 gcgtcccggt caaggaaacc gtctttgacg aggcaagcgg caaagcaaag accgtcctgc
     9301 actttgacaa acaggacaag gggcctccct ccctgaaacc caatccggca ggccgtcctg
     9361 tttccgaggt ttttctattc ggccacggga aaatccatga agtcgagcat gaaaacgtgg
     9421 gtgtggaggg cggccataag ggggaggaac ttgccgagcg gcaggccgga aaagccatcc
     9481 gcagcaccat ccggcaccac aggctgaaac cctactggga cgcggaaaag gccgagcgca
     9541 ggctattgtc tgccaacgcg gagtatttct accagaaatc tttgcgggat aacccggaga
     9601 tcgcacaggc ggccagcaac cccatttccc ggttctggca gaaacagcag atcaagcgcc
     9661 agtacgccaa ggccgcccgg acagcgggcc agaccgcagg cgcggcggca cagggcgcgg
     9721 ccaagacagc cggggcctcc acgaaagcgg ccaaggccgc agccaaagaa agtgaacggg
     9781 cggcctcctt tgtggcccgc cactggaaag gggcgctggc cgtccttgtg ttggggcttt
     9841 tgatgctgct tatcatcggc ggcttacagt cctgcaccgc catgttcggc agcgccggaa
     9901 gcggcatcac ggcgtcctct tacttttccg aggatagcga tatgttggcc gccgaggacg
     9961 cctacgcgga gctggaagcc gagctgcaag actatctgga caactacgag gccacccatg
    10021 actatgacga ataccatttt gaccttgacg agatcagcca tgacccctat gtgctgattt
    10081 ccattctgtc ggcgctccat gacgggatat ttaccatcgg ggaggtacag ggtgatttgt
    10141 ccatgctctt tgacaagcaa tatatcctga cagaaaacgt cgtggtagaa acccgttacc
    10201 gcaccgaaac cgatacatgg acggacgcgg agggcaacac ccatacagac acctaccaag
    10261 tgccgtatga ctattacatc tgctacgtca cgctggaaaa cttcgacctg tcccatgtgc
    10321 cagtctatat catggacgag gaaacgctaa gtttctattc cctgtatatg tccacgctag
    10381 gaaaccggcc tgatctgttt tccggcagcc agtaccccaa cgcctccacc ctgacgccgc
    10441 cgacctacta cgacgtaccc ccggaggctt tggaggatgc cacctttgcc gccatgctca
    10501 aagaggcgga aaaatatatc ggttatccct atgtgtgggg cggcagctct cccagcacca
    10561 gctttgattg cagcggcttc atttcgtggg taatcaatca tagtggatgg aacgtgggac
    10621 ggctgggggc gcagggccta tataacatct gcacccctgt ttccagttcg caggccaagc
    10681 ccggcgacct tgtatttttc aagggaacct acgacacccc cggcgtgtcc cacgtcggcc
    10741 tgtatgtggg caacaatgtg atgctgcatt gcgggaaccc gatttcctac acgaacctta
    10801 cttcaaacta ctggcaatcc catttttaca gttacgggcg tttaccttaa cgccagaaag
    10861 gagctttcaa ttatggcaac caaacttgac cgcatcgaaa aggacatcca gaaaaccaag
    10921 tctaagatcg cggagtttca gaaacagctt cgggagctgg aaacgcagaa aaccgaacag
    10981 gagaatttgc agatcatcca gcttgtgcgc ggcatgaaca tgaagccgga ggaatttgcc
    11041 gcgttcttac gcagcggcgc aatgcaggcc gcccctgccg caacccctta ccatgaacag
    11101 gaggacaatg cccatgaaga ataagaccag acggatttcc cgtattctcc ccgcacttct
    11161 ggccgtgatg ctctgcgtca cggcctttcc cgttaccgcc cttgcaggcg gcaatgaccc
    11221 ggcaccggcc ccgctgccgg aggctacaac cgaacccgct acgggcggtt tggagcctga
    11281 aacagacgaa accggcaccg ccacggacgg gacagaaacc gaggccgaaa aagataccgg
    11341 ccctctgacc ggcaaggaca tcaccgacct gttttccgcg ctgttcggtt ccaaggtgag
    11401 cattgccgcc acggacgacg gcatccagat caccaccggc accgacaaaa aagaacctga
    11461 acagaccggc accgtcacca ccaacggcgg caggctgaat gtccgcaccg gcgcggggct
    11521 tgacaagacc gcgttcaccc agcttcccaa tgggacgacg gttgaggtag tcggcacaga
    11581 cggcgactgg attaaaattc tcttgccgga gcgcatcggc tatgtccatt ccgactatat
    11641 gaccgtcagc gaaaaggaag tagcggctac cggcgagggc ggtttttccc tctccattga
    11701 cccggaggaa attgcttctc tgctggaact gttcaatggc ggcggggcca gcgccgccct
    11761 gtccccggac gggaacctgt cactgattga cgacatcggc agcgccgcga aaaatggcaa
    11821 gcagtttatc accgtggaaa ccaaaaacgg caatgtgttt tatctgatta ttgaccggga
    11881 cgacgaggga aaagaaactg tccatttctt aaatcaggtg gatgaagccg atctgctgac
    11941 gctgatggag gacggggaaa ccgtcacccc cgctgctgtc tgctcctgta ccacgaaatg
    12001 caaggccggg gctgtgaaca cgaattgccc ggtatgtaaa accaacctga ccgagtgcag
    12061 cggcccggag ccgcaggaac cccagccgga ggaacccgaa gccccggagg aagaacccaa
    12121 gggcggcgcg ggcggcctga tcgtgttcct gctggtggcg ctggcgggcg gcggtgcggc
    12181 cctttactat ttcaagttca aaaagcccaa ggctgaaacc accggcagcg acgatttggg
    12241 cgagtatgat tttggggagg acgaggattt ggaggacgag gaacccgaaa ccgagtttga
    12301 cccggaagct gattttgtga gtgaacagga ggacgaggaa tgacatataa acttgtaatc
    12361 gcggaaaaac ccagcgtggc ccgcagcatt gcgggcgtga tcggggccac ggagaaacac
    12421 gacggctatt tggaggggaa cggctatctg gtgagctggt gcatcggaca tctggtgtcc
    12481 tttgccgacg ccggacgcta tgacgagcgt ttcaaaaaat ggcgctatga ggatttgccc
    12541 attctcccgg aaacatggca gtacatcgtc cccgacgaca agaaacagca gtttgacgtt
    12601 ctctgttcgc tgatgcagcg ccccgacgtg accggccttg tgtgcgccac cgacgccggg
    12661 cgcgaggggg aattgatttt ccgtttcgtc taccagatgg cgggatgcca aaagcccttt
    12721 gagcgccttt ggatttcctc tatggaggac agtgctatta aggacggctt tgcccacctg
    12781 aaaccgggcg gcgactatga tccgctgtat caatcggccc tgtgccgggc caaggccgac
    12841 tggctgatcg gcattaacgc cacccggctt ttttccgtcc tgtaccacaa gaacctgact
    12901 gtgggccgcg tccagacgcc gaccttaaaa atgctggtgg acagggacgc caagattacc
    12961 gatttcaaga aagagaaata tcacatcgtc catatcacgg cgggcggcgc ggacgcggta
    13021 agcagccgct tttccgacgc tgccgaggca aacaccgtca aggcggcttg tgcaaaagcg
    13081 caggccgttt gcgtttccgt gacgcgggag aaaaagacgg aacagccgcc caagctctat
    13141 gaccttacca ccttgcagcg ggaggctaac cggcttttcg gctataccgc caagcagacc
    13201 cttgactatg cccaggcact gtatctaaag aagctgctga cctatccgag aacggatagt
    13261 caatatctta ccgacgatat gctgcccgcc gtggaaagcc ttgtgtccgg cctgtgggag
    13321 ctggtgccgt ttgccaaggg cctgaacctt tccccgcagt ttgaccgcat cctgaacagc
    13381 aaaaaggtgt ccgaccacca cgccattatc cccaccgctg aatttgtgaa gcagggcttt
    13441 ggcactctgg ccgagagtga aaaaaagctg ctgtccctga tctgctgcaa gctcttatgt
    13501 gccgtggccg ggccgcatat ctatgaggcc gtcaccgcca catttacctg tgccgggcag
    13561 gagtttaccg ccaagggcaa gaccgtcctt tcccccggct ggaaagagat tgaacgccgg
    13621 ttccgttctt ccttaaagac cgacgccgac gaggacgccg aggcggtgcg ggagctgccg
    13681 gagctttgcg agggccagac ctttgcagac gtggccgcct ctgtcaccga gcatttcacc
    13741 acgccgccca agccctacac cgaagatacg cttttgtccg ctatggaacg ggccggggcc
    13801 gaggatatgc cggaggatgc cgagcgcaag ggattaggca caccggccac ccgcgccgcc
    13861 attctggaaa agctggtgca gatgggcttt gtccagagaa aaggcaagca gctcattccc
    13921 accaaggacg gcatcaatct ggctgtggtg ctgccggagg cgcttacttc tccccagctt
    13981 actgccgaat gggaaaaccg gctgaccgag atcgccaagg ggcaggccga tcctgacgaa
    14041 tttatggacg gcatcgagac gcaggcgcgg gagctggtgc agacctattc ctgtatcagt
    14101 gaggaaaagc agaaattgtt tcaggccgag cgtgttccta tcgggacgtg tccccgctgt
    14161 ggtgaggctg tctatgaggg caagaaaaac tactactgcg gaaaccgggc ctgccagttc
    14221 gtcatgtgga agaatgaccg attttttgag gaacgcaaga aagcgttcac cccgaagatc
    14281 gccgctgcgc tgctcaaaac cggcaaagtc aaggtaaagg gcctctattc cgtaaagacc
    14341 ggcaaaacct acgacgggac ggtgcttttg gctgataccg ggggcaagta cgtcaactac
    14401 cgcattgaac acaggagcta aaggacagta acacagcaag catagggagt gtggctacac
    14461 tcccggtaaa atcccccggc cgcgtccctg ccgggccggg gatttttgct tgttggggaa
    14521 gtccccaacc ccctattttt tgaaacagaa cagagaaagg agtgaccgcg tgaacgaagt
    14581 attttctatc caagtccata accggcagtt tttggaacag ggcaaacaag gcgtctggct
    14641 ggatttgccc accacaaccg aaacgctgca agccgccctg cgggatgttg gcattactgc
    14701 cgacaatccg caggattttt ttatcaacgg gtattcctgc ccggaggaca gacaccttgc
    14761 cattccctat gacatggtgc tggccgccga cgtggacgag ctgaatttcc ttgccgcccg
    14821 gctgggccag cttgacgccg ccgagatcgc agagctgaac gccgccctgc aaaatccccg
    14881 tggcggcttt gagaatatcg gccagatcat cgactacacc gaaaatgtgg attactatgt
    14941 gcatcttccc gacgttaggg gggatggaca gttgggcgac tactacctga accgttccgg
    15001 catggtggat atgccggagg aatggaaagc cgggattgat acggcgcagt ttggacgcca
    15061 cattgcccag caggaacaag gcgtttttac ccagtacggc tatctggtga gaagcggcga
    15121 cgaatggcag cgcgtccatg agggccagcc ggtgccggag gaataccggg tgatgcacta
    15181 cccccagccg gaagctgacc gggaggaacc gcaggcccag cagccggagg catccgaaag
    15241 ccccgacaaa gcgccgcccc cggttatccc gatcatgctg gacggcatga acagcaaaga
    15301 acgcatgaaa gtgattactg accgtctgga aaccggcgta caggagcttt ttgaaagtag
    15361 ccgctatatg gcttacttat ccgctatgtc caagatgcac gattacagtc tgaacaatac
    15421 gctgctgatc gccatgcagg gcggttcgat tgtggcgggc tataacaggt ggcaggatga
    15481 atttgaccgc catgtgaagc ggggcgagaa aggcattaag atactggccc cggccccgtt
    15541 caaggttaag cagcagatgg agaaaaccga cccggccacc cagcagcccg tgatcggcgc
    15601 ggacggcaag cccgtgaccg aagaacagga agtgacggtt ccggccttta aggtggtgac
    15661 agtctttgac atcagccaga ccgagggcaa ggattttccc atgccctata tcagtgaact
    15721 taccggcgac gtgcagcagt atcaggattt ctttgccgcg ctggaacgga catcacccta
    15781 tcccataagc attgagccga tttcagacgg ggccaagggc cgctgcttct acgcaaaaca
    15841 gcgcattgcc atcaacgagg gcatgagcga attgcagacc ctaaagaccg ccatccatga
    15901 gattgcccat gccaagctcc acgccattga ccgggaggcc cccgccgagg aacaggcggc
    15961 ccggccagac cgcagcaccc gcgaggtaca ggccgaaagc gtggcctata ccgtctgcca
    16021 gtattacggg ctggacacgt cggactattc ttttggctat gtggccgatt ggagcagcgg
    16081 cagggagtta tccgagctga aagcctccct tgaaaccatc cgggccacgg ccagcgaatt
    16141 gataaccgag atcgacggcc attttgccca gcttcagcag gagcgcgagg cccggcagga
    16201 acaggaggcc gtcacggttg acgttacccc ggaggcagcg gccccggcgc aggccgagga
    16261 agaacaggcc ccggcccttg atccggcagc cgagccgctt gtgactgtgc tatggagcga
    16321 aagcccccag ctaaaggacg gccagcaaat gccgttgcat gaggccgacg cgctgtttgc
    16381 ccgtctggat gcagcatatc cggcagaggc aggctatgac aaaaccgcgt tccgcattga
    16441 ctgtatcttt cagggcgagc cggacagcta cgaggggcgg caggattttg gagataggga
    16501 cggttccctg atcgaacata tccagagcta ccaccaattt tacgcgcagg acgaaaactg
    16561 gaaaaagcat gtaatcgcta caaccggcat agagggctgg gagcaggaca aggcccatcg
    16621 ggatatgctg ttaggcgagt ttgtccccta tatgaagctg cattgcagcc ttgcccaaat
    16681 ggaacagacg gcgcagggcc gcttacagtc cggggaaacc ctgacgccgg aggaaacggc
    16741 ctatttgggg gcgcttctct cttatgtgca ggagtgccgc cccatgctca atcagggcca
    16801 gtatcagttg ccggagccgc cgaaactggc tgacttcgac cagagcttgc aggactacaa
    16861 agcacaggta caggccgaga tcgcgcagga ggccgccgca gccggtatga ccgtggagga
    16921 atacgcggcg gctggctatg aagcacccca acaggacacc ttttccatct accagcttcg
    16981 aggcggggag gaaacccgcg actatcggtt tgagccatac gagcgtttac aggccgcagg
    17041 gctggccgtg gacagtgcca actatgacct tgtttatacg gcaccgctgg ataaaagcac
    17101 gtctttggag gatatttacc gcacattcaa catcgaccac ccggcagatt ttaggggaca
    17161 cagcctttcc gtttccgacg tggtggtgct gcaccagaat ggacaggaca ccgcccacta
    17221 tgtagacagc ttcggctata tggaagtgcc ggagtttttg cgggaaaatc ccctgcgtac
    17281 cgccgaactg tctacggagc agaacgaaaa catgattgac ggcattatca acaatacgcc
    17341 gcccgaaccg cagccggagg aacccgaaca gctatctttt gaggatgtgg cagttgcttc
    17401 cggccagaca caggaggaac ccccggaggc tgcgcccccg caaacggctg tccgctatta
    17461 tcccatcaat gagggagccg cccgccgcgc caaggaagcc atgagctttt ctgactaccg
    17521 gcccggcagc gccacggcgg aataccggca ctatgtcgat caggccgtgg aaattgcgga
    17581 gcgccagaaa aagcgtgtgg agccagagta tcacgaaaag atagacagcc tccttgacac
    17641 ctacgcccgc aagctggccg agaacatgaa taagggctat gagattaccg cccgcgtccc
    17701 ctctgtcctg attgccgggc cgtccaattt cccggtacgg gcgaaagaaa agcagaacgc
    17761 cgccagtgac cgcaacatgg aggaatttca gtacattcag ggcttgcttg acaaaatccg
    17821 cagcaccggc atgggtggta tcagcgccga cgatccgcag gccgtttcca agctggaaaa
    17881 gaaactggaa aagctggaag cgtcgcagga gttgatgaag gccgtcaatg cctactaccg
    17941 caagcatggg acgctggacg gatgccccca tctgatcgaa agaggcattg aaaacctgaa
    18001 agcggatatg gcgtcgggct ggcactatga gaaaaaaccg ttccagtcct ggcagctctc
    18061 caacaataac gccgagatac gccggttaaa gggccgcatt gaggaattga cccgccaaaa
    18121 ggaggcggcc tatgtgggat gggagtttga cggcggcact gtggagatca accgggtggc
    18181 caaccgcctg caaatcttct ttgaggggaa gccggatgct gctgtacgcg acgagctgaa
    18241 aagcaacggt ttccgctggt cgcccaaggc cggggcatgg cagcggcagt taaacgacac
    18301 cacaatccgc gtcgctgacc gtatcaagtg catccagccc ctttccggcg aaaagcccag
    18361 cgccttacag cttgcggccc gcagggagaa agaaaagccc tctatccggg cgcagctcaa
    18421 cgcggccaag accgaacagg ccaagcagcc accggcgcgg gaaaaatcaa agggattgga
    18481 ggtagagtaa atgcagaagt ttgaaaatgt ggacgttctg gccgcgctgg aacagatcat
    18541 gcggcagaac accgcgtttt atcaaagtga ctttggcatt gacaaagaca ttatcaggcg
    18601 ggatgccgcc agcgatcagg ccgcagacaa gaccctgctc tggatgtccc ggccctccgg
    18661 gacgtactgt ttccgggaac gtgatgtttt cctgaaagac acccgacagt acaacacatg
    18721 gaaattttac ggggagcaga cccgtgacaa gatactggcc tacgcggtgg agctgaccgg
    18781 cacacagggc ggcactatcc ggggcaatct ctatgagctg gactatccgc agcatttccg
    18841 gcacgtcatt gaaaccgccc agccggtgag tgtgaaccgg ctcttttatg agcatggcac
    18901 ccgtgacatc ccggagagcc agcgttttga cggaagccct gaccgggtat tgggaaactt
    18961 tttgtactat gaggcccagc cccatgaccc ggctgtgctg caggaggcgc tacggacgga
    19021 gcagcacagc cgggggcggg ccgtccccgg cgatttcaaa gtccacgtca ccgctttgca
    19081 tgacagccgg attgaaaccg aggcccggcg tattgtggac agcctgaaag atctgtccgc
    19141 gcctaacagc cccaataaaa cgcatttcat ggtggagatc tccccgcact ttgcggcgat
    19201 tgcttccacc aaggataatg accggctctt tgccatgctg ccctataaat ccctgtgttt
    19261 taccgggatg aaaggcaggc atggcgtgta tgccgtgatt aaccgggacg agcgccggga
    19321 tgtgagtatt cgcaggcccc ggccctctat ccgcaagcag ctatccgaca gcaaacaggc
    19381 cgccagccca aaaaaggcgg cggccagaac caaaaagaat gaattggagg tataaggaat
    19441 gatgcacttt accgtggagg aagaaaacct gatctgtatg taccacaatg cagaccggcg
    19501 cagaacgata aacaggctca ccgccgtcat gccggttatg gatggggata tgcgggcgca
    19561 ggcccagcag accctttcca agctggaacg catgacggat gccgactatg acggccagaa
    19621 gttccacttc acagacgaat aacgaacagc agacgccggg cgcggcggcc atgtggtcgc
    19681 ttgcgtccgg cgtcttttct ttttgcctat tcctacgcat ttagaacccg gtattgtcgg
    19741 ggataggatg aagtattcgc ttgcccctgc aaacatgcgg gagaagcctt gcagcgggcg
    19801 gttttcaatc ccttaaatgc gtaggttgca gctataatta gacccctgaa acagatatag
    19861 ccgcgcagcg ggtgtatctg tttcaggggt gtgcaggtga tagccgcaaa gcggcgtaag
    19921 agggtgcagc cctcttgccg tctatctctg ttccagcggg aagctcggca atgcttccag
    19981 cagtttcagg gaaatgcgct gccgtatatc ttcgtcaatg actttcttct ttttgccgtt
    20041 tgcctgtctg atctccaccg tggaaagctc gttgatgtag tcagcataat gggccaacac
    20101 tttttccgtg gcccacctct caccggcaac ggcggctttg atcgtttcat agtccagcag
    20161 cttttcttgt ccgtccatcc tgataccccc gtcagactga ttttttctat gataccatat
    20221 tcggaagcat ggaaccagag aaaacggcgc ttttctgctg gtaaattcta cgcatttaga
    20281 acagcacttc ggcggggata ggataaaaca cccacatacc ccgctgacca tgccggaaaa
    20341 gccttgaaat ataagggctt ttacagctct aagtgcgtag gtttccagta tcttttccgc
    20401 tggcgctcgg cctctttgcg gcggcgcatc cgtttggagc aggccgcgca gtatttggcc
    20461 cggttagact tcggcgtgaa cgtggccccg cactcacagc agcgtttcac ttcgtcccgg
    20521 tctttggtga tctcggcata cagggccgca tccagcggga ggacagcggc cttaaaccag
    20581 cggcacagaa gcgaatagga aatgctctgt acgcaaatac attcttcgcc gtcgtccagc
    20641 aacaggcagt tgccgtcaca atagttgcag cactcatgcg tcagcttccg ggccttgcga
    20701 tattgagggt aattcattcg tggaatactc atagctcttg cgtccattcc tttcccggcg
    20761 tcatacgcaa aatgccgtcc acattctgct tgatgatgcc gtattcctgc atctgctttt
    20821 tcgcttcgcc gtacttctca tacagccgtt ccttttcctc ggccaatcgc ttgtactcgg
    20881 ctttcagggc gtacagatcg gggagctttt tcacaccctg tccctttagg gcctttgccg
    20941 cagcttcata caaaatcaac gtgctttcat gctcacgccg gaattttgcc ttatccgctg
    21001 cattgcggta ttccatagcc acgggccaga gctgcttgta ggtagaaata ttctttatca
    21061 gcaccgccat atccgcaagc cggtgttcca tagctttgag ggtagcggcg gcctcgtcgc
    21121 tggcggcggt gatctcggcc actttgcttt ccaaatccgg gtaggtgtct atcccatgtt
    21181 cagtcaggaa attcaaagtc tttgcggcct gcttcaaatt gtggagcttc gcccacctct
    21241 catagcctgc cgactgctgg gccttaatgt tgttttccag atcaatccgc aaagaaatcc
    21301 ccttgcgctc tttgggcgct ctggtgataa cgcggccttt gatacgctct ttgatggctt
    21361 cctcggtgta ggcttccccc aatgtcttgc agcgggtaaa gcgttcctgt ccgggagcgc
    21421 ggaaagaaat aaactttccc ggcttgatct catagccctg cgcctgcatg agccgcagga
    21481 aatcatcaaa atcttttgcc tgcgggatag cggcgtcaat ggctgctttg agcttcgctt
    21541 tccagctcgt ccctttccgc tgggcgtccc actcggcata gctcttgccc ttactctggc
    21601 cgggcttcac cacggacaat ccgttttcct tgcacagcct gtcgctggtg cgccggatat
    21661 aggcatagct ctgtttgttg gaaatgtact tgcgctgatt taccatatcc acggcacaga
    21721 aaataatgtg attgtggacg tggcccttat caatatgggt agtcagcaca tagggatatt
    21781 tcccttgcag cacttcctcg gcaagctgct tcccgatctc atgggcctgt tccggcgtgg
    21841 cttcgccggg ggcaaaggat tgtatcaggt ggtggcccag attattgccc ttttggtatg
    21901 cctgcgccag cagcatttca aattcaatgt ccgctgtttc ataagagcag gcaaaggagg
    21961 ccaccagcag cttgccgtca gttttgtccg ggttctcgat gtagtcaagg gcttttttca
    22021 gcgttgactt gataggatgt atctttgtaa ctgccatatc tgcgccagcg ccccctttat
    22081 atcctcgatg tcctgtgcgt acaccggccc cataccgttc agcctctttg caatctgatt
    22141 gacgttgacg ccgatcttct gtatctcggc ggtctgcgcc cggatgtcgc tggtgtccat
    22201 gttgacgata tagccgtcaa tgagcatctt ccgggcgtag gctgaaaagt tcttcgtatg
    22261 cagcagcgcc attttctgtt caatcatagc ccgttcctcc ggcgtcacag ggacgcgcag
    22321 cacgattttt cgctttctgt ttgccatgct tcaccgtcct ttccattcag gggtttgggg
    22381 atttccccaa caagcaaaac ggacgcggcc cggcagggac ggggcaggcc gtttttccgg
    22441 gagtggctac actccctatg cttgctacgt tactgtttct ccactaacaa gacgtttcca
    22501 aagccccaaa ctacccgcaa aatagccctc tggaaagaaa aaagtgcaaa aaaatagaag
    22561 ctacgctgtg cagctcccgc taaaagtaaa acagaggatg ccagttacac atcctccgtt
    22621 tcattcttct tcggcttttc aattagtatg gcgctgtcaa tcgcgccctc gatgatcggc
    22681 aggtattctt cggggcagag tttcagcttg tggctgaccc tttgccgcag ttcgctttcc
    22741 tcccgcatga tctccgggtt aaagtagcgt tccacaggaa gcccgcaggc tttaataagc
    22801 tgtatgacaa ccggcagact gggaatggcc cgtcgttttc gatgttggca agatagcgcc
    22861 agtcaatatt tattttctct gccattgctt tgcgcgaaac gcgctttgct tgccgggccg
    22921 ctttcacatc cgcgccgaaa gtttcaaagc cgggacaatc ttcaatattc gccatatcgc
    22981 acctcccact tacattgtat atttcatact ctgcccgtgg aatgttggta tatgcacctt
    23041 tcttaaagtt tatatttcac attttgtttc ttttattctt cgagcaaaag aattataatg
    23101 taccctgtgg aggtgtacta tggactacat gacgttgaaa gaggcaggcg agaaatgggg
    23161 tgtgccccca cgatggataa actactattg tgctgcggga cgcatccccg gcgctgtaaa
    23221 gatggcgacg atctggctca ttcccaaaac tgcggaaaag ccgattgaca ggcgtacaaa
    23281 gaaaggaaag gcggttactg aatagtataa tgaaaccggc gcattttgga tatggttttg
    23341 aagagagaga aagcatggta ttttgtcacg gaaaaactat aagatgatag atggcaggct
    23401 gctccagacc gataaaaagt attctcatct gaaacagaaa gaaaagattt acacatggat
    23461 gtatgaagaa gcgaaacttt atcatgacag aaatggaaaa tgcccgaaac aaagacgagg
    23521 aagctgctgc tgtatcagcc gtatatggta gaattgaaaa ggccggcatc tggattcttt
    23581 atggtgaagt ctccagacat tggataactg ctggtctaat gcgcctttga gtatggggtg
    23641 attgatttta ataccgggga ggtacggctt tttgaggaaa atattctggg atttacattt
    23701 ggcgattatt acatttcctg taactgcaag gacacggatg atgaagatat gaggaatata
    23761 gacttgctga ttttctactg tcttgaacgg tattaggact gaatctgctg cgcggagtgc
    23821 ggcaagagcg accggaggga gtacacacag gccctgcctg tgtgaaaagc cataccgcaa
    23881 ggtgcattcg caaaaaagag gccgcgcatt ttgctgaggc ttggggtgtc cccaacaagc
    23941 aaaacgagaa cgccgcaggc gggcaagtgg tggatcagaa tatccgtaaa ggatattctg
    24001 atccattgct tgccagtatt atcgggatgt tttgacaacc gctgtaatcg aaacatccca
    24061 aacattaaga aggtgttaat tattattatc ttgatttaat tcgtttaaac caataatata
    24121 gatacataca ccatgaaaag aagcgagtat cccaatgacg gatgattaaa atccgaaaag
    24181 aaatcgcttt gaaactacag ggaaactaca gactgttatg ttatcttctt aaaatggagg
    24241 gatttttatg tcgatacgaa ttctacttgt cgaggatgat gatcatatct gtaatacagt
    24301 aagggcgttt ctggctgagg caggatatca ggtggatgcc tgcacagatg gaaatgaggc
    24361 atacaccaag ttttacgaaa acacttatca actggttatt cttgatatta tgctgcccgg
    24421 tatgaacggg catgaacttt tgcgtgaatt tcgtgcgaaa aatgatactc ccattctgat
    24481 gatgacagcc ctgtcggatg acgaaaacca aatccgggcg tttgatgcag aggcagacga
    24541 ctatgtaaca aagccattca agatgcagat tttactaaag cgggtggaag ccctgctgcg
    24601 gcgcagcggt gcgctggcaa aggaaatccg tgtcggcagg ctgacacttc tgccggagga
    24661 tttcacggta ctttgtgacg gtacagagct gcccctgaca cgaaaagaat ttgaaatcct
    24721 tttgctgctg gtgcagaaca aaggcagaac cttaacgcat gaaatcattt tgtcacgtat
    24781 atggggatat gactttgagg gtgatggcag cacagtccac actcatatca aaaatttgcg
    24841 ggccaagctg ccggaaaata tcatcaaaac cattcgcggt gtaggatacc gattggagga
    24901 atcataataa tggaaagaaa agggattttc attaaggttt tttcctatac gatcattgtc
    24961 ctgctactgc ttgtcggtgt aacagcaaca ctgtttgcac agcaatttgt gtcttatttc
    25021 agagtgatgg aattacagca aacagtaaaa tcctatcagc cattggtgga actgattcag
    25081 aatagcgata ggcttgatat tcaagaggtg gcagggctgt ttcactacaa taaccaatcc
    25141 tttgagtttt atattgaaga taaagaggga agcgtactct atgccacacc aaatgccaat
    25201 acatcaaata gttttagacc caactttctt tatgtggtac atagagatga taatatttcg
    25261 attgttgctc aaagcaaggc aggtgtggga ttgctttatc aagggctgac aattcgggga
    25321 attgttatga ttgcgataat ggttgtattc agccttttat gcgcgtatat ctttgcgcgg
    25381 caaatgacaa cgccgatcaa agccttagcg gacagtgcga ataaaatggc aaacctgaaa
    25441 gatgtaccgc cgccgctgga gcgaaaggat gagcttggcg cactggctca tgatatgcat
    25501 tccatgtatg tcaggctgaa agaaaccatc gcaaggctgg aggatgaaat cgcaagggaa
    25561 catgagttgg aggaaacaca gcgatatttc tttgcggcag cttctcatga gctaaaaacg
    25621 cccatcgcgg ctacaagcgt tctgttggag ggaatgcttg aaaatatcgg tgactacaaa
    25681 gatcattcta agtatctgcg cgaatgcatc aaaatgatgg ataggcaggg caaaatcatt
    25741 tccgaaatac tggagcttgt cagcctgaat gatgggagaa tcgtacccat agctgaaccg
    25801 ttggacatag ggcgcacggt tgccgagttg ctgcccgatt ttcaaacctt ggcagaggca
    25861 aacaaccagc ggttcgtcac agatattcca gccgggcaaa ttgtcctgtc cgatccgagg
    25921 ctgctccaaa aggcactatc caatgtcata ttgaatgcag ttcagaacac gccgcaggga
    25981 ggcgaggtac ggatatggag tgagcctggt gctgaaaaat gccgcctttt tgttttgaac
    26041 atgggcgttc acattgatga tactgcgctt ccaaggctgt tcaccccatt ctatcgcatt
    26101 gatcaggcgc gaagcagaaa aagtgggcga agcagaaaaa gtgggcgaag cggtttagga
    26161 cttgccatcg tacaaaaaac gctggatgcc atgagcctcc agtatgcgct ggaaaacacc
    26221 tcggatggcg ttttgttctg gctggattta ccgctcacat caacattgta aatatttaaa
    26281 acttaaatga ttttgggctc tttgtcaaat aggactgata gagtgagata agaacatcta
    26341 aggtagaatt ccttttctac tttagatgtt ttttgattca taggtaatca gattatttag
    26401 taagaaaatt ctggtcttca ttttccgaaa gcttcgaaag ccataagcga ctcgtttcaa
    26461 tgctttgatg tgggtatgca gattttctat tttggcattt gaataaggca attcgagggc
    26521 taaacgaatg ccggattcat agtttataaa agcttttttt atagaatgca gttcttttgg
    26581 aacggaattg ggcatcgttc ttacaagttt aaagaaagtg tccgcctctt tgtcgtgaaa
    26641 cgcttgaaga atttcttgat agtaaaaata ggcgattcgt aattcgtcag agatcgaaag
    26701 cagacgatcc accacttcac tttcggtgag atacgcccaa ccaaaattgc gccatttggt
    26761 ccatttgtca taaacaagct tgtcctgtcg tttgatcaga agccgagaca acgcctttaa
    26821 ttgtcgataa tatttggctt gtgttggatc gtttcgatca aagcttttca tgattcgtac
    26881 gcgaatatcc tcaaatttac gaatgagatg tttgacaatg tgaaaacgat ccacaacgag
    26941 ttttgccttt ggaaaacatt cctttatcag agaggcataa gaaacattca tgtccgtcac
    27001 gatgaccttt accttcaatc gtgcgcgtcg ttcaaattgc ataaaataag aaacgagagc
    27061 ctttttcttc cgtgaaggaa gaatatccag taattttcca gtatcgccat ccatacaaga
    27121 aaaggagtag ggaccgactt gatttttcag tgaacgaaat tcatccatta ataaacaacg
    27181 cggtaaccaa tttcttttta cttttgtcat ggggatcaga gaagcgagtg tccgagaaac
    27241 ggtcgaccaa gaaacgccgc aatgcttagc aattgtcgtc attgcttgtt tttcagttag
    27301 ctcttctaaa attttatagc gcacttgttt agcgataaag cagtttctat cgacaatgta
    27361 ggtattcgcc gtaaaatagg tgtggcagcc acgacagtgg tagcgttgtt tcgctaaagc
    27421 aagataagtc agttggttcg cacatttatt aagttgaatg agcgattttt tctttccgtt
    27481 cttaacgatc atgccttcat gattcatctg tttacacaaa ggacagcgac gaatttcttt
    27541 acttaacgtc gcagaaacaa caagactctt gatattctta atgctcttcg attccacatt
    27601 tgtgatgaaa atgtttttat ctttgattcc aagtagtagt ttgatatgat gaagatcagg
    27661 catacagaac gctcctttga ttgtgttgtg gtgattcaat tctaacggag cttctgtatg
    27721 ttttgttttt tcatgctcaa aagggaccga tagaatgatt ttctatcagt cctagaaatt
    27781 atagagccga ttttgaccga caggtataac cctgccggtc tttttttgct gctacaggaa
    27841 aactacagac tggctacagg gaaagtacag atgcgcttgc cataataaca atcgtaccag
    27901 ccacaaatcg tagttttatt gcaaaggagg cattcgatca aatggaaaaa agcaactatc
    27961 attccaatgc ggatcatcac aaacggcata tgaaacaatc cgtggaaaag cgggcttttc
    28021 tatgtgcgtt cattatttcg ttcacagtct gcacgctgtt tttggggtgg agactggctt
    28081 ccgtattgga ggcaacacag ataccgccca tccctgcaac tcatacaggc agcagcactg
    28141 acgtagtgga gaatttggag gaaaacgctc ttgccaccgc caaagaacag ggagatgaac
    28201 aggaatggag cctgatttta gtgaacaggc agaaccccat ccccgcacag tacgatgtgg
    28261 agcttgagca actatcaaat ggtgagcgga tagatattcg gatttctccc tatcttcaag
    28321 atttgtttga tgccgcaaga actgatggag tttacccgat tgtcgcatcc ggataccgaa
    28381 caacagaaaa acagcaagaa attatggatg aaaaaattgc cgaatataag gcgaaaggct
    28441 acacctctgc acaggctaaa gcggaagcag aaacttgggt ggccgtgccg ggaacgagcg
    28501 agcatcagct tggtcttgct gtggatatca atgcggacgg aattcattca acaggcaacg
    28561 aggtttatag atggctggat gaaaacagct atcgttttgg ttttattcgt cgctacccac
    28621 cagacaagac agagataacc ggtgtgagca acgagccgtg gcattaccga tatgttggca
    28681 tcgaagctgc cacagagatg tacaaccaag gggtttgcct tgaggaatat ttaaaaccag
    28741 aaaaatgaga aaaggatgtt atgttatgga cagaaaaaga ttgacacagc gtttcccgtt
    28801 cctgcttcca atgagacggg cgcagagaaa aatgtgcttt tatgcgggaa tgagatttga
    28861 cggctgtcgc tatgcgcaga cgatagggga aaaatcgctt tcccatttgc tctttgaaac
    28921 ggattgcgca ttatacaacc acaataccgg atttgacatg atataccagg aaaacaaggt
    28981 gttcaactta aagctggcag caaagacctt aaacggctta ttgataagac cgggggaaac
    29041 cttttctttc tggttgttcc tgcatacgcc attgacaatt atccagcgca gaggtcacga
    29101 agtaaaggag tttccagagc caaacagtga cgagattaaa ggggtggatg caaccatttc
    29161 agagggctgg attgatttaa aagtgcgaaa cgataccgac tgcacctacc aaatatgggt
    29221 gaccttagat gatgagaaaa tcatcggtca ggtgttcgcc gacaaagagc cccaagcatt
    29281 atacaaaatt acaaatggca gtatccagta tgtccgtgaa agtggcggga tttatgaata
    29341 tgcccaggtt aaacggatgc aagttgcctt aggtaccggg gaaataatag attgcaagct
    29401 gctttataca aacaaatgca aaatctgtta tcccctcccg gaaagtgtgg atattcagga
    29461 ggagaaccaa tgagaaaaag tgtgggcatt actgtttttg gatgcgaaca ggatgaggca
    29521 aatgctttcc gcgctttatc gccggatttt catattatcc ctacgctgat tagcgatgcg
    29581 atatcggcag acaacgcaaa attggccgct ggcaatcaat gcgttagcgt aagccataag
    29641 tccgaggttt ccgaggcgac aattcttgcg ctgagaaagg tcggggtaaa atacatttct
    29701 acccgcagca tcggctgcga tcacattgat acgactgccg ccgagagaat gggaatctcg
    29761 gttggcacgg ttgcgtattc gccggacagc gttgcggatt atgctttgat gctgatgctg
    29821 atggccatac ggggtgcaaa acccaccatg cacgccgtgg cgcaacaaga tttcagattg
    29881 gatcgtatcc gggggaaaga actgggggat atgactgtgg gagttattgg aaccggccat
    29941 atcgggcaag cggtcgtcaa aaggctgcgg ggatttggat gccatgtgct ggcctatgat
    30001 aacagccgaa aaatggatgc agattatgtc cagcttgatg agcttctaaa aaacagcgat
    30061 attgttacgc tccatgtgcc gctttgtgcg gatacccgcc atctgatcgg tcagaagcaa
    30121 attggagaga tgaagcaagg cgcattttta atcaacaccg ggcgcggagc gcttgtcgat
    30181 accggggcgc tggtggaggc gttggaaagc ggaaagctgg gtggtgcggc actggatgtg
    30241 ttggagggcg aggatcagtt tgtttatacc gattgctcgc agaaagtgct tgaccatccc
    30301 tttttgtcgc agcttttaag gatgccgaat gtgatcatca caccccatac ggcgtactac
    30361 actgagcgtg tgctgcagga tactacagaa aaaacaatca ggaattgtct caattttgaa
    30421 aggagtttac agcatgaata gaataaaagt cgcaatcatc ttcggcggtt gctcggagga
    30481 acatgatgtg tcggtaaaag ccgcaataga aattgctgcg aacattgata cggaaaaatt
    30541 cgatccgcac tacatcggaa ttacaaaaaa cggtgtatgg aagctatgca agaagccatg
    30601 tacggaatgg gaagccgaca gtctccccgc catactctcc ccggatagga aaacgcatgg
    30661 gctgcttgtc atgaaagaaa gcgaatacga aacacggcgt attgatgtgg ctttcccggt
    30721 tttgcatggc aaatgcgggg aggatggtgc gatacagggg ctgtttgtat tgtctggtat
    30781 cccctatgtg ggctgtgata ttcaaagctc cgcagcttgc atggacaaat cactggccta
    30841 cattcttaca aaaaatgcgg gcatcgccgt tcccgaattt caaatgattg ataaaggtga
    30901 caagccggag gcgggtgcgc ttacctaccc tgtctttgtg aagccggcac ggtcaggttc
    30961 gtcctttggc gtaaccaaag taaacggtac ggaagaactt aacgctgcga tagaagcggc
    31021 aggacaatat gatggaaaaa tcttaattga gcaagcgatt tcgggctgtg aggtcgggtg
    31081 tgcggtcatg gggaacgagg atgatttgat tgtcggcgaa gtggatcaaa tccggctgag
    31141 ccacggtatc ttccgcatcc atcaggaaaa cgagccggaa aaaggctcag aaaatgcgat
    31201 gattacagtt cccgcagaca ttccggtcga ggaacgaaat cgggtgcagg aaacggcaaa
    31261 gaaagtatat cgggtgcttg gatgcagagg gcttgcccgt gttgatcttt ttttgcagga
    31321 ggatggcggc atcgttctaa atgaggtcaa taccctgccc ggttttacat cgtacagccg
    31381 ctacccacgt atgatggccg ccgcaggaat cacgcttcct gcactgattg acagcctgat
    31441 tacattggcg ttaaagaggt gataagtatg gaaaatggtt ttttgttttt agatgaaatg
    31501 ttgcatggcg tccgttggga tgccaagtac gccacatggg ataacttcac aggaaaaccg
    31561 gtggatgggt atgaggtgaa tcgcatcatc ggcacaaagg ccgtggcgtt tgctctgcgc
    31621 gaggcacaaa tccatgcggc tgcgcttggc tatggcttgc ttttatggga tggatatcgg
    31681 ccaagaactg cggtggactg cttcctgcgt tgggcagcgc aaccggagga caatctcaca
    31741 aaagaaaaat tttaccccaa tatagagcga gccgagttga ttacaaaggg ttatgtggcc
    31801 tcacaatcca gccatagccg tggaagcgca attgatctta cgctctacca cctggataca
    31861 ggggaacttg tttcaatggg aagtaacttc gattttatgg acgaacggtc gcatcataca
    31921 gcaaaaggga taggggatgc agaggcacaa aatcgaagat gcttgcgtaa aatcatggaa
    31981 agcagcggat ttcagtctta tcgctttgaa tggtggcact ataagttgat tgatgagcca
    32041 taccccgata cctattttaa ttttgccgtt tcataatgaa agtattggtt ttgtatcaga
    32101 ttttggattt gataaaatct atgctggtca tggacaatct tttactggaa agtggttaaa
    32161 gcatcttttg taatacattg caaattatag tattcaatct gcccagcggc agaaaagaaa
    32221 aaaaccgctg ttgggcagac ctttcccacg cccattttgt attttgctgg cggcggtttt
    32281 gagaaatcaa ggccgccgtt cttttctggc agcaaaaggt gtggcggccc ataggaggat
    32341 gccgctatgc tgttttgcta ttgccatgct ctaatccact tccaccactc aaaaaaagcc
    32401 ttgcccctc
//
LOCUS       DAYYCW010000019        30914 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000019 DAYYCW010000000
VERSION     DAYYCW010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.019"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..253
                     /locus_tag="ACHAYA_000867"
     CDS             <1..253
                     /locus_tag="ACHAYA_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8604692.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(351..1934)
                     /gene="abc-f"
                     /locus_tag="ACHAYA_000868"
     CDS             complement(351..1934)
                     /gene="abc-f"
                     /locus_tag="ACHAYA_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM8604693.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(2039..2167)
                     /locus_tag="ACHAYA_000869"
     CDS             complement(2039..2167)
                     /locus_tag="ACHAYA_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604694.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(2512..2655)
                     /locus_tag="ACHAYA_000870"
     CDS             complement(2512..2655)
                     /locus_tag="ACHAYA_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM8604695.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(2878..3357)
                     /locus_tag="ACHAYA_000871"
     CDS             complement(2878..3357)
                     /locus_tag="ACHAYA_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8604696.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3430..3714)
                     /locus_tag="ACHAYA_000872"
     CDS             complement(3430..3714)
                     /locus_tag="ACHAYA_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604697.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3792..3986)
                     /gene="ssrS"
                     /locus_tag="ACHAYA_000873"
     ncRNA           complement(3792..3986)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHAYA_000873"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(4058..5344)
                     /locus_tag="ACHAYA_000874"
     CDS             complement(4058..5344)
                     /locus_tag="ACHAYA_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM8604698.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            5482..6153
                     /locus_tag="ACHAYA_000875"
     CDS             5482..6153
                     /locus_tag="ACHAYA_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM8604699.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            6119..6607
                     /locus_tag="ACHAYA_000876"
     CDS             6119..6607
                     /locus_tag="ACHAYA_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM8604700.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            6620..7567
                     /gene="prmA"
                     /locus_tag="ACHAYA_000877"
     CDS             6620..7567
                     /gene="prmA"
                     /locus_tag="ACHAYA_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM8604701.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            7568..8332
                     /locus_tag="ACHAYA_000878"
     CDS             7568..8332
                     /locus_tag="ACHAYA_000878"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM8604702.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            8565..10778
                     /locus_tag="ACHAYA_000879"
     CDS             8565..10778
                     /locus_tag="ACHAYA_000879"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM8604703.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            10789..11235
                     /gene="dtd"
                     /locus_tag="ACHAYA_000880"
     CDS             10789..11235
                     /gene="dtd"
                     /locus_tag="ACHAYA_000880"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM8604704.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11406..12665
                     /locus_tag="ACHAYA_000881"
     CDS             11406..12665
                     /locus_tag="ACHAYA_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM8604705.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(12754..13701)
                     /locus_tag="ACHAYA_000882"
     CDS             complement(12754..13701)
                     /locus_tag="ACHAYA_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8604706.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            14095..15387
                     /gene="hisS"
                     /locus_tag="ACHAYA_000883"
     CDS             14095..15387
                     /gene="hisS"
                     /locus_tag="ACHAYA_000883"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM8604707.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15402..17171
                     /gene="aspS"
                     /locus_tag="ACHAYA_000884"
     CDS             15402..17171
                     /gene="aspS"
                     /locus_tag="ACHAYA_000884"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM8604708.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            17280..18155
                     /locus_tag="ACHAYA_000885"
     CDS             17280..18155
                     /locus_tag="ACHAYA_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8604709.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            18421..20304
                     /locus_tag="ACHAYA_000886"
     CDS             18421..20304
                     /locus_tag="ACHAYA_000886"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8604710.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(20379..20663)
                     /locus_tag="ACHAYA_000887"
     CDS             complement(20379..20663)
                     /locus_tag="ACHAYA_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8604711.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(20653..21387)
                     /locus_tag="ACHAYA_000888"
     CDS             complement(20653..21387)
                     /locus_tag="ACHAYA_000888"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM8604712.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            21516..22139
                     /locus_tag="ACHAYA_000889"
     CDS             21516..22139
                     /locus_tag="ACHAYA_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM8604713.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            22331..23452
                     /locus_tag="ACHAYA_000890"
     CDS             22331..23452
                     /locus_tag="ACHAYA_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8604714.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            23459..26587
                     /locus_tag="ACHAYA_000891"
     CDS             23459..26587
                     /locus_tag="ACHAYA_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8604715.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            26606..29800
                     /locus_tag="ACHAYA_000892"
     CDS             26606..29800
                     /locus_tag="ACHAYA_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM8604716.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(29946..30653)
                     /locus_tag="ACHAYA_000893"
     CDS             complement(29946..30653)
                     /locus_tag="ACHAYA_000893"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8604717.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        10084 a         5637 c         6558 g         8635 t
ORIGIN      
        1 tacggaaggc tttcaagaaa cccttaaaac aatttcatct acgttacctc agtcgtttaa
       61 aaagaaattc acgatcttcc atcgctacca atcgggtatt tcaaatgcct ttaaggtgcc
      121 ccactcaaac ggcgtcactg aggggctgaa taacaagatc aagctgatta aacgaattgc
      181 gtttggttat cgcaactttt ataactttcg ggcacggatc tatttacaac aaggtctgat
      241 ctttgaaaac taaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
      301 ttcaaatttg tcttcgccaa cacaatttga cgaagagccg aaaaaacgtc ctagtcaatc
      361 agttggccga gcggctggcg gctgatctgc tcctgatcaa taacgagtgc ttcttggaag
      421 tgttgctgca aaaagtagcg gtcatggctg actgctagga gcgttccttg atagtcttgc
      481 agcatttctt caatttcttc tcgagcatag ctgtcgaggt gattggttgg ttcatccaaa
      541 atcaggacgt tgattttttc taaaaagagt ttcagcaacg ccaaacggat tttttcacca
      601 ccggataaat ctttgatccg tttacccaca tcgctttgat aaaagccact tttggctaat
      661 tgccgtcgag cgacttcttc gtttccagtc aatgtgatgg cgtaagccag caaacggcta
      721 tcgggatcgg caaactgcag ctgctgcggc aaataaccga tttgcaagct actacctaat
      781 ttgatggtgc caccgtaagg cttttcctca ccgagtaata atttgagtaa ggttgatttt
      841 cctgcaccat tgtcgccgat gatggctaaa tgatctccac gatagactgc aaaactgctg
      901 tctttgaata gtacttgatc agcgtactgc tggtgcaagt tttgaatgat gaaaacttct
      961 ttgccagatt ttccaccatt agacaagctt tgcagccgat ttttcggggg aggtggcggt
     1021 ttgacgagtg tcagtttcgc caggcggcgt tccagttctt tggctttttt gaagaaggac
     1081 tcattgtctc cttcatgtgc ccactgacga aaacgacgaa tcatgacttt caaccgctgg
     1141 acttcttttt gctgcagttc gtaatttttt gtcagctcag ccaagcgagc ttctttcaag
     1201 actcgaaaac ggctgtagtt tccgggatac tcaatcaatg caccttcttc cagttcccat
     1261 gtttttgtgg tgacttgatc gagaaattgc cgatcatgag aaacgaccag aaccgatttt
     1321 tttgtattct gtaaatagtt ttccaaccag cgaatactgt taagatctaa atgattggtc
     1381 ggttcatcga gtaaaagtaa atcggcatcg gctgctaaaa ttttggctaa agcgacttta
     1441 actcgttcgc ctccgctaag ttctgttaac cgtgcttcct gacggtgtgg aatccctaag
     1501 ccttgcaaga tcgctgcaat ccggtcttct aatacgtaac cgcctagttc ttcaaagcga
     1561 tgctgcagct caccgtaacg ggccagacac ttctctaagt ttgtatctgg ttctgtcatc
     1621 aatgcttcgt agcgactcat ttgctgtttg aggtcctgta tctcagcggc agaatggagc
     1681 aagtagtggt tgacggtcgc ttcggaaact gtaagttctt gttcgaccgt cccgatcttt
     1741 aagccttttt tgcgactgat aacaccgctg tcgacacctt ctctgccggt caagatttgt
     1801 aaaaaggttg atttgccagt tccgttgata ccgactaaga caattttttc accagcttca
     1861 atttgacagg ataaatcctc aaataatgga ttcccgccga ttgttttttt aatagaaatt
     1921 gcttgtatga tcatgatctc tctccttgag accactcaaa aaggccatgg ttatccatgg
     1981 cctgtgttat ttaaactcag attatcatga gaaaaatacc ttgagatggc cattgatttt
     2041 acgtgaattc aactgttttg gacaaacttg tcccgtcaac agcaactgta aaaccaattg
     2101 catggctaaa tgcgatatgg tatcgttggt aattttcatt acttgttatt ttagtcatgc
     2161 gcatcatagt attttccctc cttcgtgtag ttgtcttttc cagtgtacct gaaagtgtca
     2221 gaaagttcaa ctagcgctga catttactta atatttatcg atgtgtaatt ctgtcagaga
     2281 gcttttttta tctgactgag ggtaatgtct cgtggaccac atcattgtat tctgagctaa
     2341 accagcgttc aggcgaaatc atatggtgtt tgataaaaaa ctttgtctac atgacttcct
     2401 tttgccattt gcttaatacc gttgcattaa acagctcttc tctttggaaa tctcaccaac
     2461 atcgcgttca ggagtatcgt tgtggttgca atggcaagcg gtagcctttc atcaattctt
     2521 gaatgattta ccgaatgtag cacttgcctt ctcgcggtac ccgaacccga aataaaattt
     2581 tagtttggcg ataaaatcgg tatccgccat cgatacttca tattttttcg tatcagattc
     2641 atcggaaact tccacattga cgaaccaata agctttggca tgcttaggtc gttttaaacc
     2701 acaccagggc gactaatcgc cctggtgttc actttttttt attttacttg aactcattga
     2761 ctgttttccc tttattttaa acattataaa gaccgatgag tgcttgccgc gtcttttgtt
     2821 tttatcacgg ttttttgtgc aaaaaaacct gccgatagtt ggcaggtcgt ctacgtgtta
     2881 agctttggtg tggtctttag ctggcaattg gttttctaaa tcagttcgga caacaagcac
     2941 atcgcaagct gcgtttctaa taacatattc agaaacagaa ccaataaata aacgttctac
     3001 agcattcaat cctgtggctc ctaacataat caaatctact tgattatctt ctgggatttc
     3061 tctggcaata atttgttttg gtgagccata ttctacgaca gaggttacat tattcaagcc
     3121 agctttcttc gcattgcttt cataatctgc caatgtttgc tttgccattt cagtagcttg
     3181 ttctgcaagc attccatcaa aggatgaaac ggtttggaaa gaacgtgtgt caatgacatg
     3241 tgctaataga agttctccgt tatttcggat agctacattg accgcctttt taaaagctaa
     3301 ttcagcttca tcagaaccat ctacggcaac catgattttt ttgtattgtt gtaacataag
     3361 cattcactcc tcaagtcaat cgtattgtac tcttttattg tagaacactt tagggaaaaa
     3421 gagaattatt tactgctttg gttcagcaat tttttttgca aatcggatac tgaaggtggc
     3481 agaaacaata attacgatca gtaaaaagac caaggcaata aagcgttgat tgaaaaaagc
     3541 tgttaaaata cccattacgc ctaaaacagc atatgcagct ccgtaaaatt gtaggaatcc
     3601 ttgattttta tcatttttgt tgatcaatac aaaaaagata tcctgtttat taaataaata
     3661 atagctaacg aataataata ctaaagtggc gataatcatt aaaatcctaa tcatgaacaa
     3721 atcctccaaa ataagcaaca aatagaaaaa gcaatttgcc aagagcaaat tgctttattc
     3781 tttaaaagat ggtaatctcc gttaatgccg ttgtttgtcc tgtagtattg atcccgcgaa
     3841 ttcaagcagg tgggtcccat ggtcgtagct tcgaactacc aaatgataag gcatgttacc
     3901 agcagtgaca caagacgggt cccaagatat caataaaaat gttcggtcaa cacacaaaga
     3961 ggacaattcg aacatcacag atttcccacc tctatagtag catatgaatt taaaaagagc
     4021 aatgagagag ctcgcagaaa cagaaccgtt ttcttgtcta gttttttgga ggatgctttt
     4081 tccactcttt tatacggtaa tactgttgac ccagagcctg ttcatatttc cctgtatctt
     4141 ttggctgata atactgagca ttcttcagtt tatctggtaa atattgctga ttgacccaag
     4201 cctgatcaaa atgatgagga tactgataac ccacgccacg gttgagtgat ttcgcacctt
     4261 tgtaatggct atctctcaaa tgatcaggaa catctccagc tttgccctct cgaatatcag
     4321 caagtgctgc atctaatgcc atgtaggcag aattggattt tggcgacaag caaagatcga
     4381 cgacgacgtc tgctagtggg atacgtgcct caggcaatcc caatttttca gcagctaata
     4441 cagcgttcac tgtacgagcc gcagcagcag gattacctaa acctatatct tcataaccga
     4501 taaccatcag acgccggcaa atacttgcca gatcacctgc ctcaactaaa cgggcgagat
     4561 aatgaagtgc agcatccaca tcacttccac gaatcgattt ttgaaaagcc gaaataacgt
     4621 cataatgagc atctccattt ttgtcatggg taagagcttt tctttggaca cattcttcaa
     4681 tgatcgacag tgtaagatga atccttcctt ctttatctga aggagtcgat agtgtagcca
     4741 gttccagccc attcaatgca ctccgcaaat cgccgttcgt tgcacgcgaa aggtgaagaa
     4801 gagcatcttc gtctaactgg attgcttgtt gtccaaggcc gcgttcttta tcccttaaag
     4861 catgttcgac tgccaactgg atgtcttgtt ctgttaatgg tttgacttca aagatctgtg
     4921 tacggctgcg gatcgctgga ttgatggtaa tataaggatt ctcagtagtc gctccgatca
     4981 agatgattcg gccactttct aaatgaggaa gcagaaaatc ctgttttgtt ttatctaatc
     5041 ggtgtacttc atccaataag aggatcactg taccactcat ttttgcttct tccgctacaa
     5101 cttgtagatc cttttttgaa tctgtagcgg cgtttagcat gcgaaatgca taattagtag
     5161 agccggcgat cgcacttgcg atactcgttt ttccagtacc aggaggtcca tatagaatca
     5221 tggaagagag catgcgagca tcgaccatgc ggcgtatgat ttttccttcc ccgactagat
     5281 gctgctgacc aacgacttca tcgatcgttc gaggtctcat gcgataagcc aatggctgtt
     5341 gcataggatt ttcctccttc ttgtttttac gtcccttaat tatatcatat ttagcaagat
     5401 gcctagaaag aacagccttc tgttgttttg ttttcatagc ttatccaact aatattgtgt
     5461 atgataaagt aggtgagaaa aatgacggat aaacaaaaga tagatgaatt tttttggaat
     5521 aaaacgacgc cagaagtggc acgggattta ttggggatgt atttggagca tgacacacca
     5581 gaaggaagat tagctggata tattgtagac gcggaagcat acctaggtcc agaagacgag
     5641 gccgcccata gctatggttt aagaagaaca ccgcgtgtgc gggcaatgta tgaaaaacca
     5701 ggtacgatct acctttacac gatgcatact catcggattt tgaacatcat tactcagcca
     5761 gaagggattc ctcaaggagt aatgatacgt gcaatcgaac ccgccgcaat gattgatcaa
     5821 atgagtaaaa atcgtggcgg aaaaactggt ccagatatca gcaacggtcc gggaaaatta
     5881 gtggaagcat tagcaattcc tcaagaactt tatggtcagt ctatagcaga tagttcgtta
     5941 cgcttagttt ttgagaaaaa aaagacacca aaaaagatca atgctttgcc tagaatcggt
     6001 atcccaaaca aaggtgtatg gacagaaaag ccgttgcgtt ttgttgtgtc gggaaatcct
     6061 tatatctctt tacaaagaaa aaaccaaatc gagaaagatt ggggttggag gaaagaaaat
     6121 gaaaaagaag gatctattaa catatttaga tgaaataatt gaaaaacaag tgacagacta
     6181 tgatgtagct ttagattggg atacaaaaaa tcatacgatc gagatcgttg ttcgcttgtt
     6241 tgcagaaaat aaagcacaat ttgagattga tgatgcagaa gggatcgttt cgcaagaacc
     6301 aattatcgaa tttgaagacg gcgtacttct ttttaatcct cagaaatctg tttttgatga
     6361 acgcaattat ctagcagtaa tcccttatga aggaaaaaaa gggttggcaa aatcagtagc
     6421 tgattcttta gtggaatatc taaatgaagt attagctcaa gggcaaagcg atttgcttga
     6481 ttttttggat gaagacgatg acgaagctgt attcgagctt cactgggata atcagcaact
     6541 agcagaatta gtagaagtaa aacagcaaaa tgaagcagat acttatttac cgtacccaag
     6601 ttattaagga gtgacagata tgaaatggaa tgaagtaaaa gtagaaactg aaagtgaagc
     6661 agtcgaagca gttgctaata ttttgatgga ggcaggggcg agtggtgtcg cgattgaaga
     6721 ttctttagat gttgaacatt ttcagtcgga tcaatatggt gagattttag ataaaaatga
     6781 ttttacatcg ctgaaagaag gcgcactagt catggcttat tttccggaaa cgactttctt
     6841 accggaaatc ttgcctttta tcaaagatcg tatcacacaa ttacctgagt ttggtctagc
     6901 tattggaaaa aataaagtaa cagttagtga agtagaggaa agcaactggg caacagcctg
     6961 gaagaaatac tatcatccgg tgcgtattac gcgaatgctg acggttgcgc caagctggga
     7021 aaactaccag acagaagatc ctctagaaaa aattatcaca ctagatcctg gtatggcatt
     7081 tggcacaggc actcatccca cgacttcctt gacattgaca gcactagaaa cagtcttgcg
     7141 tggtggtgaa acattgctag atgtgggaac tgggtcgggc gtgctaagta ttgcagcaaa
     7201 atatcttggt gcaaaagatg tctatgcctt tgatttagac gaagtagccg ttcgttcggc
     7261 gaaagaaaat atggatatga atgaaatagc caaagatgtc catgtatcag ctaacgatct
     7321 tttgacagga atcgagattg aaagtgatgt aatcgttgcg aacattttag cagatatcat
     7381 cttattgatg atcccagatg cttggcgttt actgaagcaa acaggcacgc tgatcgtttc
     7441 aggaatcatt gaagagaaga agcaaatggt tctggatgcc atgttagaac aagggtttat
     7501 cgtggatcaa attttccagc aaaaagactg gtatgcaatt attttgaaaa aaccagaggt
     7561 ggaatagatg cagcggtatt ttttaaaaga atcatatgaa tcaaaagact attttgtttt
     7621 agaaggggaa aactatcacc acattgtacg ggttatgagg atggaacccg aggatgaagt
     7681 ttttttggca ttcaatgatc aaatagccat tgttgcagaa atcacggaga ttactgaaga
     7741 ggctgtttat ctgaaagaaa ttaaaaaaga agaaaatcaa aaggaactgc caagttcgat
     7801 taccatagcg agtggctatc ctaaagggga aaagctggat tggatcgttc agaaatccac
     7861 tgaattagga gcgcaagctt ttcttggttt cccggcatca gcctctgtcg ttaaatggga
     7921 tgcaaaaaaa cgtatgaaac gacaacaacg gcttgaaaag attgctcaag aggcagcgga
     7981 acaatcccat cggcagatcg tgccatcagt agagttattg gaaacgaaac aagatttatt
     8041 tgagcggcta gatgattatg acgtggtctt ggttgcttat gaggaatcag caaagcaagg
     8101 cgaaaaatca aatgcagcca aaattcttag tgaaacaaaa ccagatagtc gaatcttagc
     8161 tgtctttgga ccagaaggtg gattatcccg cgatgaagtc gattattttg tagaacacaa
     8221 agggatactt tgtggattag gaccaagaat ccttcgtaca gaaacagcac ctctttattt
     8281 attaagtgct gctagctatc aatgggaatt aatgaatcct tctttcagct aacgttcttc
     8341 attaataaaa gagtatcgac agaaagaatt gaaaaaatag tcaacaattt gtataataga
     8401 aacaattatt tatacaatgg atacgaaaaa tcgtttgaaa aagcttttaa aaagcgaaca
     8461 tttccgtaaa aaaacgtata actgcacgga ttttgggaca attatcaaac agatatttgt
     8521 cccgattccc gttttaccga aaaaagaaag ggggatcgat tcctgtgaca aaagaagaaa
     8581 ttttaacggg acccggtgtc ataaagctcg ttagtcaata catgggtccc gaacatgtag
     8641 catttgttga gaaagcatgc gaatacgcga ctgctgcaca tgacggacag tttagaaaat
     8701 caggcgaacc ctacattatt catcctatcc aagtcgcagg tatattagca gatttaaaaa
     8761 tggatcccca tacagtggct acaggcttct tacatgatgt tgttgaagat acagaaatca
     8821 ctttagaaga tctgagagaa gaatttggcg atgacgttgc tatgttagtg gacggcgtaa
     8881 ccaaattagg gaaaataaaa tataaatccc acgaagagca gctagcagaa aaccaccgaa
     8941 agatgctgct agcaatggct caagatttac gagttatcat ggtcaaatta gctgatagac
     9001 tacataacat gcgtacgttg aagcacctgc gagaagataa gcaaagaagg atcgctcagg
     9061 aaactttaga aatctatgcg cctcttgcac atcgtctagg gatcagccgg atcaaatggg
     9121 aattagaaga tacagcactt cgttatctaa atccaaaaca gtattaccgt atcgtccact
     9181 tgatgcagac gaagagagaa gaacgtgaaa aatacgtaag tggtactgtt gaagatattc
     9241 gaatagcgac agaagagctg gggatttttg cagaaatcta tggacggcca aaacacattt
     9301 attcaatcta tcgtaaaatg aaagatcaga aaaagcagtt caacgaaatt tatgacttgc
     9361 tagctattcg agtgatcgta gattcaatca aggattgtta tgctgtatta ggagcaatcc
     9421 acacaaaatg gaaaccaatg cccggcagat ttaaggatta tattgccatg ccaaaagcta
     9481 atatgtacca atctttgcat actaccgtga tcggtccggc aggaaatccg gtagaaattc
     9541 aaatccgaac acaggaaatg catgaaatcg ctgaattcgg ggttgctgca cactgggcct
     9601 ataaagaagg aaaaaatgaa aaagtagaac cagatggtat gacgaaacaa ttaagctggt
     9661 tccatgagat actcgaactt caagacgaaa gctatgatgc ttctgaattt atggaaggcg
     9721 taaaaggaga tatctttagt gataaagtct acgtcttcac accaaaagga gacgttactg
     9781 agttaccaaa aggatccgga ccattggact ttgcatacag tatccataca gatatcggta
     9841 acaaaaccac tggtgcaaaa gtaaatggca aaatggtgca gcttgattac aaattgaaaa
     9901 acggagatat cattgagatc atgacttctc caaattcatt tggcccaagt cgcgactggt
     9961 tgaaattagt tgctactagc aaagcaagaa ataagatcaa acgtttcttc aaagcccaag
    10021 atcgagaaga aaatgtgatc aaaggccacg aatccgtggt caaatgtatt acagatctag
    10081 gatttacgcc taaagatatt ttgacgaaga acaaactgca agaagcactc gatcgtttta
    10141 attatcaaac agaagatgat ctctatgcag ctgtagggta tggagaagtt agccccttga
    10201 cgatggccaa tcgtctgact gaaaaagaac gtaaagaaca aaaaatcgag cagcaaaagc
    10261 aagaagcaga agaaatcatg aatcagccga aaaaagaacc tgacaaaatg aaagtacgtc
    10321 atgaaggtgg cgttgtcatt caaggggtag aaaacttact aattcgtatc agtcgctgct
    10381 gtaatccgat tcctggtgat gatatcgttg gttatatcac taaaggcaga gggatatcca
    10441 ttcatcgtcg agattgtccg aatgttcagc ctgacaaacc aaatgtagca gaacgtttga
    10501 ttgaagtcga atgggaagat acatcgaata cacgaaaaga gtatgatgca gatttggaaa
    10561 tttacggcta taatcgttca ggcttattga atgatgtact tcaaacagtc aatgcgctaa
    10621 cgaaaaatct caacagcgtt gaagcacgga cgaataaaga taaaatggcg acgatccatt
    10681 tgacggttgg tatccagaat ttatcccacc taaagagtat cgtggataaa atcaaagcag
    10741 tacctgatgt ctacagtgta cgccggacga atggatagga ggacagatat gagagcagtt
    10801 gttcaacggg taagtaaagc tgctgtgtca gtcgatcagc aagaaattgg aaaaattggc
    10861 tacggcctac ttgttttatt aggcattcat gaaaccgata cgcaaaaaga tgtggactat
    10921 ctggtaaaaa aaattgcaca aatgcgtatt tttgaagatg aacaaggaaa aatgaatatt
    10981 agtgtagaag atgtgggagg acaaatttta agtatctctc aattcacact gtttgcggat
    11041 acgaaaaaag gaaatcgacc aagctttgtg gaagccgcac gaccagaaac agctattcct
    11101 ttatacgaat cattcaatga aggaatcaga agtcgcggca tcacagtcgc taccggacaa
    11161 ttcggagcag atatgtcggt ttctctcgtc aatgatggac ctgtaacgat cattattgat
    11221 tctaaaaaca aataaagtaa tctggttttc actagtaaaa actatgaaac acgtcgctta
    11281 agttagactg ataaactcta acttaagtgg cttttttgtc tgcaaaaagc gcttcttttg
    11341 tcagcagtcg gaaaaaaagt taagcttaaa ttatacaagt tattttttat aggaggttgc
    11401 tgaagatgaa agaagtacca gtagtagata tcaaaatcac agatttaaaa aaattgtatc
    11461 aaaaaggata caacatgttg gaggagttac gccatgaagc ggacgctccc gtcgtcaaag
    11521 caaagatttt caataaagaa gctatcacga tctacggatc atcagcagca aaagtatttt
    11581 atgatccaag gaatttcaaa cgaaaaggtg ctatgccaaa attagtattg aagacacttt
    11641 ttggtcaagg tggagtacag actttggatg gagctgctca tcaccatcgg aaaaatattt
    11701 ttatggatct aatgacaccg gaaagaatgg aagattatca tcgtatctta gataagaatc
    11761 tgacacaagc acttgaagca cagcatggtc aatttgaact atttgattta agtaagatgg
    11821 ttttctttac atctatttgc gagtgggcag gtatcaattt atcagcgatt tctaaagatg
    11881 aggtcgaaaa attagcagaa tatcaaatct ctatgatcag cggaacgttt acttctccga
    11941 ttgatcacat aaaaggtgtc gaaaatcgca aaaaatcaga aaaatgggcg caaggcttga
    12001 ttgaagaagc tcgacaaaat cctgtggctg gaaaagaaaa tgtggcatta tatgcttttg
    12061 ccaatgccac agatttagat ggtcagcttt tacctctgga agtggcagct gttgaattat
    12121 tgaacatcat ccgtccaacg gttgctctaa ctgtgtgggc ggcattgatg ggacatgcac
    12181 ttttcagtag accagatttg tatcagcaat taaaaaatga cttttcgacg ttacaagatc
    12241 cgtttattca agaaatgaga cgctattatc ctttcttccc aatgctacct gcgatttcgc
    12301 tgaaagaagt agaagtagat ggttatcgta taccagaagg cagttgggtt attttagatt
    12361 tatatggaac agaccatgat gaacgaacag ttgaagcgcc agattccttt atgatcaaac
    12421 ggtatgtagg gaaagcaaaa gatatttcct ataaagaaga atatgagatg attgcacaag
    12481 gcgggggaaa ttttcgacaa atgcatcgct gtgcaggaga atggatcact cttcatagtc
    12541 tacgtgtgtt ttccgatcag ctagtaaata agtttgagtt ctctgtacct gaacaagatt
    12601 ggacgattcc attcaatcag tttccgactt atccgaatag ccgagcgcta ctttacaaga
    12661 attagattag cataaactta tcgaaaaaaa ggaagatcaa acagtgctga tcttcctttt
    12721 ttcatattac tataaattga taaggtcaat tgatcaaaat ccacgaaatg cttcatcaat
    12781 ggtttgataa tcttcattgg ataaatgcaa atctaaagct tggacattgt cagatacttg
    12841 ctctggacgc ttagctccag gtataactac agacactcgt ggattcttca tgtaccaagc
    12901 taaaacaagt tgcgcaggag tggcttggta tcttttagca atggggcgaa gtacatcgac
    12961 agcggttaaa atttctctga aacgttctcc ttggaaatct ggatttcttt ttcgaggatc
    13021 tccttcacca aattgttttt cctcaccaag ttcatatttt cctgtcagca aaccggaagc
    13081 aagaggaaaa tagggaacaa aactgatttt gtttttttca agataaggga ataattcttt
    13141 ttcagcctgc cgatggatca aactgtattt atcttctact acatcgacat atccatctgc
    13201 attcgcttct ttcaattgtt ctaacgtgaa gtttgacaca ccgactgctc ggatttttcc
    13261 tgcttctttt aattcgtgca atgtagcaac tgattcattt tttggtgtgc tttcatccgg
    13321 aaagtggata taaaaaatgt cgatgtaatc agtttgcaga cgttttagtg catcttccac
    13381 cgattgtttg agaaactcag gagaattatc aaatgttcgc cctttatttg gtacatgagc
    13441 agcttttgta gcgatgacga cacgtgagcg atcatattct ttttccttca gtacctcgcc
    13501 aatcagttct tcggaacggc cgtttccgta agcataagcg gtatcgatca aattgatccc
    13561 gctattcaaa gctgtacgaa ctacttgttt tcctgtctca tcttccaaac cgtcaaaaag
    13621 attatgtcct ccaacggcat ttgctcctaa tccaagctgt tctgcgtaga cttgtgaatg
    13681 tccgattctt atttctgcca tagctgattc ctcctttttg tctagtctaa aacaaaatac
    13741 caaaaagctg aactaaaacg tttttgtgag gggaaatagt cagatatctc ttgactttga
    13801 gactggtttt tagtaggtta taagtaatat cgaatgtcaa tgaaagagcg agtaagtttt
    13861 tgaaacttta caagagagga tcatcgaggc tgaaaatgat ctaaagtttc attagcctaa
    13921 agacactctg gagatggacg agtgaaaagc cgtcccgcgt acgagcgtta ttcgtaagag
    13981 gaaagagaaa tctttcaaaa ttaggtggta ccgtgtatga atttcattca cccttgttca
    14041 tgatgaacaa gggtgatttt tttatttaca tagctaagaa agggagagaa aactatgagt
    14101 ttccaaagac caaaaggaac aaatgatatt ttgccaggcg aatcagaaaa atggcagttt
    14161 gtcgaagaaa cagcacgttt attattcaga gattatcagt ataatgaaat gcgtacaccg
    14221 atttttgaac actatgaagt gatatcccga agtgtagggg atacaacgga tatcgtttca
    14281 aaagagatgt atgattttta tgacaaaggg gaacgccatg tgacgcttcg accagaaggt
    14341 acagcaccaa tcgttcgttc ttttgttgag cataaattgt tcggcccaga gtatgcgaaa
    14401 ccgttcaaaa ctttttatat gggaccaatg tttcgctacg aacgccctca aaaaggacgg
    14461 ttacgtcaat tccatcaaat cggagtagaa gcatttggat cagaaaaccc tgcaactgat
    14521 gtggagagca tggtgatggc acttgatttc ttcaaacagt tggggatcac gcaaatccgg
    14581 ctagtcatca attcattagg tgataaagaa acgcgtcaag cctatcgtca agcattgatc
    14641 gactatcttt taccttttga atcagagctg agtgaagact ctaaacgccg ccttcatgaa
    14701 aatccattac gcgtattgga cagtaaagac aaacgagatc aaaaatttgt ggcagaagct
    14761 ccatctatct tagattatct aagcgagcct gctcaaaaac attttgatac cgttgtttcc
    14821 atgctggatg cattggctgt tccttatgaa atcgacagta atatggttcg cggactagat
    14881 tattacacac atacgatttt tgaaatcatg agtgacgcac caaaaatggg ggcacaagca
    14941 acgatttgcg caggcggacg ttatgacaat ctagtggaag aactaggtgg tccttcaaca
    15001 cctggcttcg gatttgcaat gggtatcgag cgattactct tgactatgga ggcagaggaa
    15061 gtagttatcc ctgcatttaa tgagttggat gcttacgtgg tcgcactcgg agacgaaaca
    15121 aatatcgaag cattgaaagt ggtccaagcg attcgaaact ttggtttttc agctgaccgc
    15181 gacttcatga accgtaaagc aaaagcgcaa tttaagacag cagataaagc gaatgccaaa
    15241 ttagtattgg tgatcggtga agatgagctt gctagtcaaa cgattaatgt caaagcaatg
    15301 gcaagccgaa aagaaaaaag ttttccatta gaagagatat atgaacattt tgatgaagtc
    15361 tatgatgaaa tgactttatt cgagtaggag gaaaaaaata aatggcacaa agaacgatct
    15421 attgcggatt agtttccgaa caacaattag aacaaacagt tacattaaaa ggatgggtac
    15481 aaaaaagacg agaccttggg ggagtaatct ttatcgacct acgtgatcgt gaaggaatcg
    15541 ttcaaatcgt ctttaatcct gaaatcaaca aagaagcatg ggaaatcgcg gataaatgtc
    15601 gcagtgaata cgtgatcgaa gtgactgggg aagttagaag acgcggagaa aatgcagtaa
    15661 atcctaaaat ggccactggt gaattcgaag tgattgcaag tgaaatcgtg attttgaatc
    15721 aagcaaaaac cccgccattt caaattgaag atgatcaagc aatcagtgac gaaatacgtt
    15781 tgaaatatcg ttatttggat ctgcgtcgtc caaaaatgac taaaaacatc aaattaagaa
    15841 atgcgacaac aaaagcgatt cgtcattatt tagatgataa tgactttctc gatatcgaaa
    15901 caccttacct agggaaatct acaccagaag gcgcaagaga ctacctagtt ccttcacgtg
    15961 tccatgctgg acatttctat gcgttgccgc aatctcctca attgtttaaa caattgctga
    16021 tgaatgctgg attcgaccgt tattatcaaa tcgttcgttg tttccgcgat gaagatttac
    16081 gtggagaccg tcagcctgaa tttacgcaag tagatattga aacaacgttc ttaacagcag
    16141 aagaaatcca aacttacaca gagggattga ttgcgaaggt aatgaaagaa gttcgcggta
    16201 tcgaagtcac attgccattt cctcgtatga cctatgatga agcaatggct cgctacggca
    16261 gcgacaaacc ggatacacgt tttgatatgg aattgatcga tctaagcgat acagtcaaag
    16321 aagtagaatt caaagtcttc caaatggctc ttgaaaacgg cggtgtcgtg aaagcattga
    16381 atgctaaagg cgctgcagat cgctactcac gaaaagacat ggatcagtta ggtcaatatg
    16441 taggacaatt cggagctaag gggcttgcat ggctgaaagt agaagaagac ggattgaaag
    16501 gtccaattgc taaattcatg ggagaagcaa cagaagcaat cattaaagca acagatgcaa
    16561 aaccaggtga cttgctgatg tttggcgcag ataaaagtga aatcgtcgct gctgcattag
    16621 gtgctatccg gacacgttta ggaaaagaat taggactaat tgatgaatcg aaattcaatt
    16681 tcttatgggt aactgattgg ccacaatttg aattttcaga agaagaaggt cgatatgtct
    16741 ctgctcacca tccattcacg atgccaaaag aagaagatat cccgttattg gctgaagacc
    16801 ctgcaaaagt ctatgcagaa gcttatgata tcgtcttgaa cggttatgaa ctaggcggag
    16861 gttctatccg tattcatact cgtgagcttc aagaaaaagt actagaaact ctaggcttta
    16921 caaaagaaca aatggaagaa caatttggtt tcttgttaac agctttagat tacggattcc
    16981 ctccacatgg cggtattgca ttaggattgg accgttttgc aatgttatta gccggagaag
    17041 ataatattcg tgaagtcatt gcctttccta aaaacggaaa agcagcagat gtgatgagtg
    17101 atgcaccaag tatggtttcg cctttacaat tgttcgaatt gaatattgat gtaacgaata
    17161 tagaagaata gccgataaaa aggtaaaaag gatttgtcga atacgcttct tttgacaacc
    17221 ttgatttaat tgaaaaacac tttataatga agccataaca tataaagcgg ggcttttcca
    17281 tgaaaaaaac attcgaaaat tgggcttatc atgattacac gaccaagctt tcggtatcta
    17341 tcgtttatgc gttgatcgca tcagtcgcat tgaatttctt ttatcagcca ggacatattt
    17401 attcaagtgg tattactggt ctagctcaga ttttgacgac tttgtcaaaa tcactgatag
    17461 gattcgaagt accggtatca acaactttgt atttactgaa tataccttta tttgtattgg
    17521 cgtggttaaa aatcgggaag aagtttacaa ttttcacttt tatcacagtc actttgacct
    17581 ccctttgtat ccatatttta ccggaaacag tcctatctac cgatccaatc atctgtgcgt
    17641 tgtttggggg ggctgtgatg ggactaggta ttgggctagc gttgagaaac ggcctatcat
    17701 cgggcggtct agatatcgtg agtatcacga ttcgtaaaaa aacaggaaag tcgattggtt
    17761 ctatttcgat ctacttcaat gcattgatcg tttttgttgc cggaattcta tttggctggc
    17821 aatatatgtt ttacagtacg ttgtctattt tcgtcagtgg aaaagtaact gatgtcgttt
    17881 atactaaaca aaagaagatg caggtaatga tcgtaacgaa acatccagaa aaagtagtaa
    17941 atgaaatcca aaactgtatg cgtagaggga tcacgatcat caatgaagca gaaggtgctt
    18001 ataagcatga taagcagaca gtattgatga cgatcgttac acgatacgaa ctgcctctac
    18061 ttagagaagc aatgaaagct tctgatccac aagcttttgt aagtattgca gaaaacgtcc
    18121 agatacttgg tcgtttctat gaggatggca tttagatcga gtttgacaaa aattcatgaa
    18181 atactgctgt agacagacgt tcgtttgtct gcagtttttt tctaccatag tagaatcagc
    18241 aaagaaaaac tgctttttta tttgaaattt tcttaaagtt gaaaaaaagc atgtttattt
    18301 ttttaaattt atgggatgat agaaaaaaat agtagagaaa ggacacgatt ctacttgcta
    18361 ttttcactta atacccgtat attacgaatg aaaaaagttt atgaaaagtt ggaatattga
    18421 atggaaaaac aaaaaaggtt aaaaaacagc cgctacatca ctgggtttga tgggctccgt
    18481 tcattagctg tggtgggtgt tatcttctat caccttctgc caactagtat gaaaggcggc
    18541 tatttgggcg ttccgatttt tttcgtcgta tctggttatt tgatcacgga tctgctgcgg
    18601 caggaatggg aacaaaatgg aaaaatcaat atttggcaat tttatgtcag aagaatgaag
    18661 agactctatc cggcacttgt ttttatgctt gtgacagctt cggcttatat cactttgttt
    18721 cagcgagggc tgttaaacaa tcttcgagga gttgtggtca gcagcttact gtatgtcaac
    18781 aactggtggc aaatcaataa cggtttatct tatttcgatc gatttgctaa tgagtcaccg
    18841 tttacgcata tctggtcttt agcagtagaa ggtcaaaact atttggtttg gccgatcttg
    18901 tttgtattgt tgatggtatt tgttcgtaaa agaaaatgga tcttttatac tgtgttaggt
    18961 acatccttcc tctccgccat cttgatgatg atcttgtata caccaggagg cgatccaacc
    19021 cgtgtttatt acggtaccga cactcgtctg ttttctatat ggttgggaag tgcgcttgcg
    19081 tttgtttggc caagtacgcg tttaaagaaa aacatcccga aacaagcgaa aaatattctg
    19141 aatatcgctg gaggaatctc gcttttggca ctgattcttt gcttcttctt tttagatgat
    19201 cattacaatt tcatctatta cggaggaatg ctcctagtga gtctgttatg cgtagtgcta
    19261 gttgcagtga cagcacaccc cggagcaagt ttgaatcgat ggctgacaaa tccagtattt
    19321 acttggattg gcaaacgaag ctatggcatt tatctgtatc agtttccagt tatgattttt
    19381 tatgaagcaa aggtgactga tattgccgat catgttttgt tgcatacttt agtggaaatc
    19441 acattgattc taatcatcag tgaactttct tatcgatatg tggagcgtcc gctagcacgt
    19501 tttgattatg gtcgtacatt ccaagtgatg aaaggatggt tcagcaaacc gatcatcagt
    19561 aagagcaaac catgggttgt tccaggcaca ttacttgtag caattgcact cgttggtttc
    19621 gttacggcac cgaaaaacgc tgtaacagcc gatcaaaaac aactgcaggc aaaaatcgct
    19681 gaaagtaaaa aattagcaga agaaaccaga aacaattcag aaaacaccac tagttcggtt
    19741 gaccaagcga tccttgataa gtacgagttg tccaaggcac aagggaataa ggcacaaaaa
    19801 ttagaactaa cggcatttgg tgattcagtt atgttggatg ctgcaacgga tctacaagaa
    19861 ctttatccaa aagtagtggt agatggagat gtcggacgac aattatatgc cagtgtaccg
    19921 tacattgaaa aattaaagga acaagatttg ttgaaagaca ctgttctgat cggactagga
    19981 acgaatggct cattctcgga agctcagttt gatagtgtga tggatgcaat cggtgatcga
    20041 aaagtatact gggtcaatgt ccgagtaccg acgaagcgct ggcaaaatga cgtgaatgcg
    20101 atgcttgaac agatgacgaa aaaatacgac aatatgacgc tgattgattg gtacagctac
    20161 agtaatgacc atagtgactg gttttatgat gatcaagtcc atccaaatcc tgaagggatg
    20221 acacattaca ttcacttagt cagcgaagcc ttgctaggag gaagttcaag tacgaacagc
    20281 agtgagacaa acgtgacaga atgatagcta caaaaaactt caggcaaaaa catttgtttt
    20341 tgcctgaagt ttttttatat ttaccgtgtt tttaacattc atatagattc aggaagcaaa
    20401 acggttgagt gtgtctgtaa ataacgttct ttttgttttc gggtgagttt tttgaatgca
    20461 gcttctgctt ttgttgcttc gcttcttgaa gaaaatactt ctgcatggat catcttaaca
    20521 ggtcttcttt tttgaagacg agtatattta gcaccagtac cattgttatg ttcctctaat
    20581 cgtctagaaa gatcggttgt atatccacca taaaatgtct gatctttaca gaataatacg
    20641 taaaaataat gttcatttgc catagagcat ctcacgaatc tctgcagtgt attctccatc
    20701 catattgtaa gtgatcaatg gtggagcgac tttgaatccg tctaatttgc cttctttgat
    20761 tccttcaact aacaaaatat ttgcctcttt tccaggtttt gggtaaacaa attggatcct
    20821 tttaggtgca attcgatagt cttccatgac ctgaatgatt tctaagaacc gatcaggacg
    20881 atggaccata gtaaagcgtc catttgtttt tagtagctta cttgaaatat cgatcacttc
    20941 ttctaacgtc gtatgaatct catgacgggc gattgccagg tgtttattag ggttcgtttt
    21001 atttgttggc agccctttga aataaggtgg attacagacg actaagtcac aagaattatg
    21061 tcgaacgaca gataagctgt ctttcagatc gatggtgtgg acagtcattt gttcatttaa
    21121 atggttaagc gcgatactac gtcttgccat gtctgctaga cgaggttgaa gttcgatagc
    21181 gtcgatagcc gcctgggttc gtttacttaa aaaaagtcca actgcgccat ttcctgcaca
    21241 aaggtcaaca atcttccctt ttttaggtac acgggaaaag tgagcaagca gcacagcatc
    21301 taaagaaaaa gaaaacactt tggaactttg gataatcttg atatcttctg cgtataattg
    21361 gtcgattcgt tcgtcttcgt gaagcatgga ttccctccaa aatgaaaaaa atagtcgtct
    21421 gtattatact atttttatta aaaagaacca aggtggcttg tcaaacctct attttttatg
    21481 catactatga ccataagaag ggaaaggaag agactatgtt ttatcgattt atgcgcgggg
    21541 tagttcgagt actattagcc ttagttaatg gaaatgcccg ctatgagaat aaagaagttt
    21601 taccgcaaga tgagaattac atactagttg ctccccatcg tacttggtgg gacccattat
    21661 atctggcggt agctgcagca cccaaggaat tcagctttat ggcaaaagaa gaactgttca
    21721 aaaatccagt tctgcgattt atattaactc gatccaatgc ttttccagtt aaaagagaaa
    21781 atccaggacc aagcgtcatt aaaacacctg taaagatttt gaaatcgaca aatttgagtt
    21841 tgatcatgtt tcccagtggg acaagacatg ctacagaact taaaggcgga atggcattga
    21901 tttccagaat gtctaaagcg aaaatcgtgc cagctgtata tcaaggtccg ttgacacttg
    21961 gggatctgtt caagcgtcag agagtgacag tgcgtttcgg cgaaccaatc gacttatcag
    22021 atatcaagaa aacggataaa gaaggtatgg cggagatcga acgacgaaca caagcagctt
    22081 ttgacaaatt ggacaaagaa gtgaaccctg atttcaaata tgaagtcaaa aaaaaataaa
    22141 atgataagtt tatgcgtggg cggtcttttg aatctactgt cctgttcagt tcagactttt
    22201 tcaatgaaaa cgagggagag acgtcaagtc tgccctcttt ttgcgttcca atatgtaaaa
    22261 tagaagacag gtgtcatgta atggatcaaa gtgttataat aattagaagg acattttttg
    22321 aggtgattga atgcgttttt tacatacagc cgattggcat atcggaaaaa aactacaagg
    22381 ttatgacttg attgaagacc agcgtcatgt actatcttct atactagata tcgcgaaaga
    22441 agaaaaagta gaggcaatcg tgattgccgg agacttatat gataggagtg tcccagctgt
    22501 tgatgccgta gaattataca atgaattgat ggtcgattgg aatttaaagg aaaagatccc
    22561 gatttttgct atatcaggaa atcacgatag cagtacgaga ctttcagcgg gtacgccatg
    22621 gttcagttat tcgaactatt ttttgcgaac acagctagca gaagcatttg aaccagtcga
    22681 gtttaaaaac gtacaatttt tctttcttcc ttactttgaa ccaattgcag cgagattata
    22741 ttttgaagat gacaatatcc gaacgatcaa gcaagcggta gccaaggtga tagataaaat
    22801 gcaggagcta tttgattcag aaaaaaaaca agtattgatt agccattttt ttgtaatggg
    22861 aagtgtaaaa tctgattcgg aaacgaaaat cgaagtaggc gggttggatt cgatccctgg
    22921 tgaactactt acaccgtttg attatgtagc actaggacat ttgcataatc aagctgcttt
    22981 gagacaggat aatgctcgct atagcggttc cccattgaaa ttctctttat ctgagatcaa
    23041 ccaaaaaaaa ggcgtatgga tcgttgacct gacaacaggc ctggagttga attttaggga
    23101 gatcgaaccc ctgcatgata ttgaagaaat aacagcgagt ttcaaagaat tgctggatcc
    23161 tgccttttat cagtccgtca atcgggagaa ttacatctat atctatctca ctgatagaac
    23221 gatcatcccg aatatgatga accagcttcg gcaaatatat cctcgtattt tggggattga
    23281 acgggcaaac ggtcgagcag aacaaacaaa gcgcaaacga aaagtactag aaatgaaaga
    23341 tcctcaaaaa ttagcagaag cattttttag tgaaatgacg gaagagcagc taactgcaaa
    23401 ccaagaaaaa tggctcggag aaacgttgca agaaatcatg gaaatggaat gatcaagaat
    23461 gaaaccgaaa aaattacaac ttaaaaattt tggtcctttt atcaatgaga ccgtagattt
    23521 ttcacgtttg acagaagcac cgcttttttt gatcagcggg aagacaggag ctggcaaaac
    23581 gacgattttt gatgggatca ccttcgcact ttttggtgaa acttccggaa gacttcgctc
    23641 tggaaaagag atgcgttctt tgtttgctac accagacgaa gaaacaagcg tgactttttc
    23701 ttttgaacat cagcagatga gctatgaaat cgaacgaaaa ccagaacaag ttttagcaaa
    23761 acgaaaagga aacggaacaa aaaaacaggc agccaaagta acgttgacca tttttgatgg
    23821 gaaaggaaaa gaaataaaac aaatcacaaa acgtacagaa gcagatcagt tgatcagaga
    23881 actgatgaat ctcgatgcga aacagttttc tcaaatcgtt ttactacccc aaggagaatt
    23941 ccgtaatttt ttggtttcat caagcagtga taaagagact gttttacgtc atttattcgg
    24001 tactcaattt ttccaacagt tcaatgagcg attaaaagaa aaagcaaaac aacagcaaca
    24061 aaaattggat catttagagc aagagctgca tcttctgcaa aaaaggtttg ttttagtcaa
    24121 agaagaggaa ccagcagcag ggtttgaagc cgttttagcc caatggacaa accatcaggc
    24181 aatactcgaa aaacaaatca cggaagaaaa agaagctcta acggaacttc aggaaaaaca
    24241 aaagcaacta gaaaattcct actatcgttt tgaaagcttg aaaaaaagtt atgaagaaaa
    24301 aacgatatta ataaacaagc aaaaggaatt agctgctcaa aaagacgaaa tagaggaaaa
    24361 gaaacgatgg attgggtatt atgaatttac gcaacgatta gtcgaaccaa tccagcgcct
    24421 acaagaaata aaagttgaaa aaatagaact gtcacaaaaa agtagtgagc aaacaaagaa
    24481 actagcgatg actacgcaga attatgaaga gtggcaaaaa aatgaagcaa accgcgtacg
    24541 ccgacaaaca aaaatcaaag aagctgctca aaaactgcaa aacaaacaga tgatggtgcc
    24601 aattgtcgaa tccttacgta gtaaaaaaca agacgcagaa caattaacaa tttcattgga
    24661 taaagaagaa ttgaatgccg agaagctgta tcaaaatctc gctatctatc aggaaagaac
    24721 tcgaattctt caagaaaatt tactggatca ggaaaaactg caagaagatc gtcttgcttt
    24781 tggacagtta aaatatctag atgaaaagca gcaagaaata gaaaaacaaa aagcaaaaga
    24841 cactgggtcg ctcgcacaga taagagaaga actcaaaaaa gcaactgaag aaataacaaa
    24901 aaatagtcag ctattgaaag aacagacaga acaagcagaa cagctgaaaa gccaatgggc
    24961 aaagcagcaa attgctcgtc ttcagctttt tttgcgtcca ggggagccat gtcttgtttg
    25021 tggatcaacg gaacacccca tacaacaatt aaaacacgaa cagccaaata aggaagatat
    25081 cttaaaagta gaacaacaac tggcagaagc tgagtctgct gtagaaagaa ctcagcaaat
    25141 cattgccaaa agtgaagcac atgaaaataa tcttcaacaa caattatcag atttagaaca
    25201 aaaaatcatg gcaacgaata tacaattggc tgaacaagaa gaacacttgc aacaggcttt
    25261 gaaaaaggca tttcctgaga taacagaaca aaaaacaaaa acaactcttg tagtaatcga
    25321 acaatcttta gatagacgaa aaagcaagat ggccaatgca agaatagagc tggaagaaat
    25381 aagacaacaa gcagaaaagc tcgaacaaca attagctgtc tacaaacagc agcagacaga
    25441 aaaaaataat cagctccagc aactgcaagg agaaatcaaa agtttagaac aacaattgca
    25501 agatacggat agtaactgtt tagaagagga gatacgacga cttgaaaaac agttagaaga
    25561 agactcgaat caactagctg ttgagcagaa agcaggagtg caattgttgc aagatcttca
    25621 tacgcttcgg acacagcttg atcttcaaca tgaacaaata gaaaaaatag atgaaaaaat
    25681 agaaaaggaa caagaaagaa tagctaaaaa gttagaaagc cagtcgtatt tttcaacaat
    25741 tgaagaaatc tgtgcatttg cacaacagca agcacaatat aaagaaaacc aacgtacagt
    25801 tacttcttat catgaagagc aattagtgac agctgaccgg ctgcgtcaat tagaaaatat
    25861 ccagctaact gagacattcc cagatacaga agagatcagc caacagttag aagaaataag
    25921 acgtatgatc gaaataaagc aagaaaagat atacggttta caagaacaaa aaattagtaa
    25981 tcaaaaaatc tacgatgaat gtctttcgat ttatcacaca agccagacga aattagatga
    26041 gcttacgcaa ttacagcagc tttcgcaaac attcaacgga gaaaatccta agaagactag
    26101 tttggaacgg tatgtgcttc aggtttatct tcaagaagtg ttacaagtag caaatgatca
    26161 tctccaacgt ttaacgaaaa atcgttatca atttgagcta gcggatacca ttggcagtta
    26221 tcgagggaaa accggattag aaatcaatat ctatgatgat caggcaggta tgtctcgaag
    26281 tgcgcatacc ctgtctggcg gtgagagttt tattgcagca ctttctttag cattagctct
    26341 tgccgatgtg atccagacac aagctggtgg tgtagcaatc gatgcattgt ttattgatga
    26401 aggatttggg tcattagatg aagaagcttt ggaaatggca atggaagctt tagaaacgat
    26461 cgaaagtgaa ggaagaatga tcggcatcat tagtcatgta agagaattga aagaacgagt
    26521 attacaacaa ataagaattg atacagaagg tagcggacaa agcaaaatac gctatcaact
    26581 tggataaaaa gggagattgg taactatgaa atggagtatt ccagaaaaag tcattgcacg
    26641 tggacgtcaa tacttgcaag aaggccgagt gttatcggtt gtccctgacc aagaagaaaa
    26701 cgtgtggcat gcagaagtct tgggcagtga attgtatatg gttgatttgg atggtacagc
    26761 aaaagagata gattactgcc aatgtcccta ttgggaggaa cataaatatt gtaagcatac
    26821 agttgctgtg gaactttatt tacggtcgaa aaatctatct cgtatcatga aaaaagacca
    26881 gccaatgcta gaaccggtaa aagcaacaag tgagggagaa atcattgcaa aaggtttttc
    26941 tcgtctaaaa acacaaaaca atcctcaagc ggtgcaacct ttgattattg aataccaagt
    27001 agaaacgatt gagactaatc agtatcaccc agagctttct attttggcac tttacttgcg
    27061 tatcggttca ttggcatcac cgaaaaaaac atatatcgta aaaaacatct atgatttttt
    27121 acaagcttac acagaaaaag aaacttacac tgttaataaa cagtaccagt ttcgtctaga
    27181 taaacgggcg tttcaaaaag aagatcaaaa aatactcacg catcttgcag gaagtgctca
    27241 gacgcatcaa ttattaggta cgaacggctt acaagtcaaa ggaaaactag ataaaagata
    27301 tctgcttctc ccagtagaac aagcccgctt cctgttagag cagatgaatc agactacacg
    27361 tttgcatctg cagataaatg aacaaaacga tcaaggaata caattttccg atcaaaacaa
    27421 acctttatca ttcaaagtgg aaaaatttgg agaagattat ttgctgcaag cattgaatga
    27481 ttttgatttt ttctttatgc attatcagtg gggagttatc caagggacat tttattctct
    27541 gacgaaatat caacaaacgg tttatcaaac ttggaaacaa ttgattcgcc gattggagaa
    27601 accagaagta gtgttcaaga aaaaagaatt accgtctttg tttaaagaga ttatccctct
    27661 gctgtcagag atcggcgacg tagctattgc ccctgcagta gaagaggaag tagcagatat
    27721 ccccgtcgaa tttattttct ttttccgaaa agcaaaagga aaaatccaag ctcgagtcga
    27781 tttcgtctat ggcgaagtca tctattcgac tgacccgaag catgaggtct cgacagaaca
    27841 ggcttttcag gtgttacgtg acacagtaga agagaaacga gtacttgatc tattcagaat
    27901 gtatcgctac cagaaaaatg aaactggtta tgaacgtcca ttgccaagcg gggaagaact
    27961 atatgccttt ttccgcacag aactatctgt cttccgtcag tttggtgaag tcagacttgg
    28021 aagaaaattg cgagagttgt atttagatgc gcataaacat caaccgaaaa tcgaagtaac
    28081 cgagaccggt tcatggctcg atattcgatt cgatgtgaca gggatagagg agcaagaaat
    28141 cgatcatgtc ctgcaaagct tgctgagaaa tgatgctttt tatacattag aaaatggtca
    28201 ggttctttct tttgattcag aagaatttca acaaacaagc caagtgttgc aacaattacg
    28261 ggaatcgata cgtacagaag aaggaacgat ccatgtacct aaaaatcaag gattgatcat
    28321 tcaaaatcaa ttggaaaaaa gcaatgctac ttttagtgaa tcgttccaaa cgatggtaca
    28381 agatctcatt catccagaaa gataccaggc acagcttccc aaaggattga atgcaacgat
    28441 gagagattat caaaagcaag gttttcgatg gttgaagatg ttaggtcatt atcagtttgg
    28501 cggtatttta gccgatgaaa tgggacttgg gaaaacacta cagacaattg cctttttact
    28561 ttcggaaaaa gaagaacgga aaagcttctc tgctttgatc gtagcgcctg cgagcttaat
    28621 ttacaactgg caggcagagg tgagaaaatt cgccccttct ttaagtatcc aagtaatcaa
    28681 tgggaataaa aaagaacgtg aagaattact ggctaaagac acagatatcc gagtgacttc
    28741 ttatgccagc ttaagacagg atctagctaa ttatcagtcg caaaaaattg attatttgat
    28801 tcttgatgag gcacaaatgg tcaaaaatag cagtacgaaa actgcacaag cattgagaga
    28861 attagctgta cctcagcggt ttgcactgag tgggacacca atcgagaata atttagaaga
    28921 attgtggtca ttgtttgcaa cgattatgcc aggatttttc ccgactaaaa caaaattcag
    28981 ggaacttgcc gcagaagaaa ttgcgcaaat gattcgaccg tttattttac ggcgagataa
    29041 acaaactgtg ttgaaagact tgccggaaaa aacagagatg aatctatatt ctgcacttac
    29101 agaagaacaa aaaactgttt atttagctta cttgcgtcaa atgcgtgaag aaatcacatc
    29161 aatggattct gaagcattca agaaaaaccg gatcgggata cttgctggtc tgacaagact
    29221 gcgacaaatt tgctgcgatc ctcgtctgtt tattgaagat taccaaggcg gttccggcaa
    29281 gctcgaacaa gtaaaagatc tactggtagc tgccaaagag aacaaacgaa gagttttgct
    29341 gttttctcaa tttacaggga tgctgactat cttgcaagaa gagttagccg agctagggat
    29401 ctcgacattt tatttaagag gcagtacgaa accacaagat cggttaagta tggtagacgc
    29461 atttaatgct ggcgaaaaag atgtttttct gatttcctta aaggctgggg gaactggatt
    29521 gaatctcacg ggagcagata cggtcatctt gtatgacctt tggtggaatc ctgccattga
    29581 agaacaagct gctggacgtg cacatcggat cggacaaaaa aatgtggtag aagtgtggcg
    29641 tatgatctct gaaggaacga ttgaggaacg catggattca ctccagcaag aaaaacgcga
    29701 actgttccaa aaagtgatcc aaggtaatga agaacagttg acgaaactga ctgaagaaga
    29761 catccgcatg attttaagtg taggagaaat cgaggagtaa gagactcgaa aaagcgttca
    29821 gtaatgggta agaaagcctt tctaaagaag cgattgttta tttgatttaa aaaatagcaa
    29881 ggaggctggg attccatttt atcccagctt ccttgctatc atttttttat ttctattttt
    29941 aaaggttata caaagattgt cttcattgtc tctaacagct gatcaaaaga agaaatctcg
    30001 atatcgcaaa ttggagtctg ttcaattttg cgttctcgat ggttgaacca aagcgcttgc
    30061 cagttagcac tttttgcacc tagaacatca ttgtcgtaat tatcgccaac gtataaggtc
    30121 tcttctggaa gcatatgaaa ttctttctca gctagctgaa aaatctcttt ttctggtttt
    30181 tggaaacctg ttgcttgaga aatgagcata ttgcgggaag gaatccaatt gttcaattga
    30241 agttgcttca gtttttttgt ctgatggtca gtcggcccat ttgtgataat tcctaatggg
    30301 acattctttt ccttcaagaa atcgagggtt tttttgacct cctcatgcaa gcagatatta
    30361 tcaagttctt cctcgtaaat tttttgaaat aagaggccat cttcttccgt aatatgaggg
    30421 tagtctaaat cttttagtga ttggctgatc cgatgtgctc gcatatattc tagtgtccaa
    30481 tctcctgcca ttacttttgg gaagttttca tcactatgat ggcggaaccg gatatatagt
    30541 ggatgcatat ctgcatctga aacaagcgga acgactcttt tgaccgcgtt tcgaaatggc
    30601 tgctgctgat catacatcgt atcatcaaca tcaaacacaa ctgatttaat caaaatttta
    30661 cacgcctttc ggttttcatt gattttttca caaatccttg actattgtaa cagaaaaaat
    30721 tgtctaaaaa agctgataaa taaaggtttt tcttactata tttctatttc cttgacagaa
    30781 taaggttttt atatttcagt ttttttcact aaaaatcgtg aaacacgtca ataagtttga
    30841 tgaacaaatt aatcattatg gtagaagtcg attgatttat tttaatgcaa tctctgtgga
    30901 attttcgaaa gatt
//
LOCUS       DAYYCW010000020        30101 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000020 DAYYCW010000000
VERSION     DAYYCW010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.020"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACHAYA_000894"
     CDS             complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACHAYA_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM8604718.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACHAYA_000895"
     CDS             complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACHAYA_000895"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM8604719.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2298..2960)
                     /locus_tag="ACHAYA_000896"
     CDS             complement(2298..2960)
                     /locus_tag="ACHAYA_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8604720.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3144..4790
                     /locus_tag="ACHAYA_000897"
     CDS             3144..4790
                     /locus_tag="ACHAYA_000897"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8604721.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4781..5863
                     /gene="uxuA"
                     /locus_tag="ACHAYA_000898"
     CDS             4781..5863
                     /gene="uxuA"
                     /locus_tag="ACHAYA_000898"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8604722.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5971..7524)
                     /locus_tag="ACHAYA_000899"
                     /pseudo
     CDS             complement(5971..7524)
                     /locus_tag="ACHAYA_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7672..8813
                     /locus_tag="ACHAYA_000900"
                     /pseudo
     CDS             7672..8813
                     /locus_tag="ACHAYA_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8939..10018)
                     /locus_tag="ACHAYA_000901"
     CDS             complement(8939..10018)
                     /locus_tag="ACHAYA_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8604723.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10121..10561)
                     /locus_tag="ACHAYA_000902"
     CDS             complement(10121..10561)
                     /locus_tag="ACHAYA_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8604724.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10618..11028)
                     /locus_tag="ACHAYA_000903"
     CDS             complement(10618..11028)
                     /locus_tag="ACHAYA_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8604725.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11208..11642)
                     /locus_tag="ACHAYA_000904"
     CDS             complement(11208..11642)
                     /locus_tag="ACHAYA_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8604726.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11742..12590)
                     /locus_tag="ACHAYA_000905"
     CDS             complement(11742..12590)
                     /locus_tag="ACHAYA_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8604727.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            12979..14745
                     /locus_tag="ACHAYA_000906"
     CDS             12979..14745
                     /locus_tag="ACHAYA_000906"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM8604728.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            14847..16106
                     /locus_tag="ACHAYA_000907"
     CDS             14847..16106
                     /locus_tag="ACHAYA_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8604729.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            16253..17554
                     /locus_tag="ACHAYA_000908"
     CDS             16253..17554
                     /locus_tag="ACHAYA_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8604730.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            17557..18408
                     /locus_tag="ACHAYA_000909"
     CDS             17557..18408
                     /locus_tag="ACHAYA_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8604731.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            18421..19236
                     /locus_tag="ACHAYA_000910"
     CDS             18421..19236
                     /locus_tag="ACHAYA_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM8604732.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            19387..20553
                     /locus_tag="ACHAYA_000911"
     CDS             19387..20553
                     /locus_tag="ACHAYA_000911"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8604733.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            20783..21430
                     /locus_tag="ACHAYA_000912"
     CDS             20783..21430
                     /locus_tag="ACHAYA_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8604734.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            21514..22458
                     /locus_tag="ACHAYA_000913"
     CDS             21514..22458
                     /locus_tag="ACHAYA_000913"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM8604735.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            22593..24044
                     /locus_tag="ACHAYA_000914"
     CDS             22593..24044
                     /locus_tag="ACHAYA_000914"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM8604736.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            24065..24901
                     /locus_tag="ACHAYA_000915"
     CDS             24065..24901
                     /locus_tag="ACHAYA_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8604737.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            24901..25560
                     /locus_tag="ACHAYA_000916"
     CDS             24901..25560
                     /locus_tag="ACHAYA_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8604738.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            25686..26375
                     /locus_tag="ACHAYA_000917"
     CDS             25686..26375
                     /locus_tag="ACHAYA_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604739.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            26381..27337
                     /locus_tag="ACHAYA_000918"
     CDS             26381..27337
                     /locus_tag="ACHAYA_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM8604740.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            27381..27893
                     /locus_tag="ACHAYA_000919"
     CDS             27381..27893
                     /locus_tag="ACHAYA_000919"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8604741.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(28149..28697)
                     /locus_tag="ACHAYA_000920"
     CDS             complement(28149..28697)
                     /locus_tag="ACHAYA_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8604742.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(28786..29967)
                     /locus_tag="ACHAYA_000921"
     CDS             complement(28786..29967)
                     /locus_tag="ACHAYA_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM8604743.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         9977 a         5457 c         5723 g         8944 t
ORIGIN      
        1 tcttgtcacc atcctttttt ccgctctctg actttttaca atcttcatag ctcaagtgcg
       61 catcagataa tcgaatgctc ctaaagctgc agtagctcct gaacccattg aaacaatgat
      121 ttgtttgtat ggactatctg tacaatcacc agctgcgaaa acgccgggga tattcgttgc
      181 gccatttttc tcggtcacta tttcatttct tggattcatt tcgatcgttc catctagcca
      241 atccgtatta gggagtaagc cgactagaat gaatactcct gaaacagcta gtgtatgcat
      301 ctccatcgta cggcgatcga tataggagag agattctacc tgattcgtac cgctgatttc
      361 tttcgtcgct gcatgtttga taacagtgac attcgcaaga gagactagtt tatcctgtaa
      421 gatttgatct gcttttaatt ctggtaagaa ttctagaaca tatacatgtt ttgctaaccc
      481 agccaaatcg attgctgctt caacaccaga attaccacca ccgataacag caatctcttt
      541 tcctttaaat aaagggccgt cacagtgtgg gcaataagca attcctttat ttttaaactc
      601 tttttcgcct ggtacattga tagaacgcca gtgcgcacct gtcgcgatga tggcggtttt
      661 tgctttaagt gatataccgt tagctaagcc aatattaata aaatgttgct ctttatttaa
      721 ggaaacggcc tgttgattca tcataatgtc gatagggtaa ctacgcacat gactttctac
      781 ttgctgcatc aattttggac cttctgtgta agaggtacca atcatatttt caatccctag
      841 tgtttcaacg acttgtccgc cgaaagaatc agcgacaagt cctgtacgga ttcctttacg
      901 tgctgcatag atagctgcac tactagctgc tggtccaccg ccaatcacta atacatcata
      961 aggttctttt tcaaacaact cttcttcaat caaagggcca gtgatttttt caacaatttg
     1021 ttcgatcgtc atgcgtccgc tactaaattc ttctccattt aaaaacacag ttggcactgc
     1081 catgattttt ttatcatcga tttctttttg gaacatccca ccttcaatca tcgtatgagt
     1141 aatatttgga tttaatacag ctaatatatt caatgcctgt acaacgtcgg ggcaattatg
     1201 acaagtcaag ctaacataac tgtcaaaatg caatgtttgt gttatttttt tgattttaag
     1261 actcaaagat tcactgattt tcggtgcccg tccactaatt tgtaacatag ccagtaaaaa
     1321 agaggaaaat tcatgaccga gggggatacc agcaaaaacg attccacttt ctttgccaac
     1381 tggattgata gaaaaactag gtgtgcgcat cgaatctctt ttttcccaag agattttagg
     1441 agacatttct gctatttcct tcgtaaattc agctagtttt tgactatttt cactattatc
     1501 caaactaaca gaaaaaacaa taggagattc aatcagtgct aaatattgtt cgagattttg
     1561 tttagtttct ttgtctaaca tatccaccac ctaaatcttt ccaacaagat caaaacttgg
     1621 tttcaatgtt tcgccacttt ctttccattt tgccggacaa acttcacctg ggtgggtacg
     1681 tacatattga gcagcgcgaa tcttatcaat cagtgcacta gcgtcacgac cgattccatc
     1741 tgcattgatt tccatagctt gaacgattcc gtctggatca ataataaatg ttcctcgctg
     1801 agctaatcct tgctcttcat ccaaaacatc aaaaatacga gaaatctgat gagacggatc
     1861 accgatcatc acgtattcga ttttaccgat agcatctgat gtatcatgcc atgctttgtg
     1921 agtaaagtgg gtatcggtag agcaagaaaa gacttcaaca cctaaagatt tcaatgtact
     1981 gtattgttct tgtaagtctt ctaattcagt aggacaaaca aaagtaaaat cagctggata
     2041 gaagcaaaga atactccagt tccccataag atctttttca ctgacttcta aaaattctcc
     2101 ttgatgataa gcatgtgcat taaattcttc gatttttttc ccaatcaatg acatagtaaa
     2161 acctccaata ataattattt atttataatt attgtaattt agttggagtt gcaaagtcaa
     2221 tggatatttt gttatttaat aaagattact caaaaacgaa ggttaattat aaaaatagaa
     2281 gaggtcgcaa gaaaatgcta ctgctggaaa tactcgggat aacgttgagt aacagtttcc
     2341 tgttctttca gcatcatgtg taagtgcaat cccgtttgaa atcgagcttc ttctggtttt
     2401 ttatcaacta aagccttgag taaatcataa tgttgttcca tgatcgtttc ccatggaagc
     2461 ccctgaatgg tcactcgtaa ccagcggaaa cgttctaaat gggtattatg gtcatctaac
     2521 cagttccaaa cttcttttct tcctgcaatt tcgaaacaag cttcatgaaa taaattatct
     2581 gcacggaaaa aagcgcgttc gtcatttatt ttgactgctt tttcttgttc ttctaaaatc
     2641 gtttctagca ctattcgtgt ttgtgtgtcc aatttggaac aacattcaat catgatttgc
     2701 tcttccaact gttctcggac aaagcgagca ttttctgcag aaacaagatt aattttggaa
     2761 acatacgtac cactttgagg tatcgtatac acgagttctt gctgacgtag ttgaatcaat
     2821 gcttcgcgaa tgggagtccg gccgatagca agatgttcct ccagtgcctt ttcagatatt
     2881 ttccttccag gttctaagtc agcatagata atttgtttac gaatcgcttg ataagcttgt
     2941 gattgcaagc tgcttttcat ctttattcct ccaaaatcat ttctatgaac gtagtataac
     3001 agaagaggtc tgaaaaacaa tctggtatgt cacattaact agtaaatttg tataaatagt
     3061 aaaattttgg ttgacatagc gatttttatt gcataaaatt cacttgaata caaaaactaa
     3121 tatgttagtt taggagggga aaaatgattg atttaaacaa ctggaggaac tttatccctg
     3181 aattatctca aaaagaagta gaaatacctc attttgaccg tgcatcatta agagaaaatg
     3241 gacaaaaaaa tcctgtctgg atccatttcg gtggcggaaa tctttatcga ggatttcatg
     3301 cagaaatcgc tcaacgttta gcggatctag gcgaattgtc cactggtgtc attgtttgtg
     3361 aaacattcga tgaagaaata attgaaaggg cttaccaaga ttttgatgat ttgattcttg
     3421 aagtggtcat gccaagcagt ggagagttcg tcaaacgtat attagctgcg acagctgaca
     3481 gtctgtattg tcatccagat tgttctgaac actttgataa gatgattcga cactttgaaa
     3541 atcctagttt acaattagtt acttttgcta ttactgaaaa aggttatcga ttgaaagata
     3601 caaataataa atatatagga ccgattgcag tcgatataaa aaatggtcca aagaagccat
     3661 cacatacaat gagtattctt gctgtgctac tttataaacg ttttttatct ggagcttggc
     3721 cgattgcact tgtctctaca gataattttt cacaaaacgg tagtcaatta cgtacttctt
     3781 tgctaacgat cgcaaaagaa tggcaaaagg aaggttatgt acaaaataca tttatccaat
     3841 acttatcgaa tgaagaaatt gttgcgtttc catggacgat gattgatcgt atcaccccga
     3901 atccttcctt gtcagttagc aaaatgttgg caaaagaggg cttttcaaat atgtcgattt
     3961 tccaaaccgc aaaaggaaat aatattgctc catttgtcaa tacagaggac atccattatt
     4021 taatcatgga agatcatttt cccaatggac gtccgaaatt cgaaaaagct ggggtattct
     4081 tgactgatcg tgcaacggta gataaagcag atacgatgaa ggtgacgact tgccttaatc
     4141 ctttgcatac gtctttagct attatgggtt gtttactggg gtataggagc atagctgagg
     4201 agatgaaaga tccagattta tcgctgctag tttctgaaat cgggtacaat gaaggcctcc
     4261 cagttgtgga gtctccaggt atcattgatc caaagatctt tctggaagaa gtaattactg
     4321 tgcgcttttc taatccaaat attcctaata tgcctcagcg aatcgccacg gatacctctc
     4381 aaaaggtggc gattcgttat ggtgagacga ttaaaaaata tatggaaata gaagttaaag
     4441 acccgcagac acttgtattc ataccattga ctctcgctgc ttggcttcga taccttattg
     4501 gcattgatga tcaaggaaaa ccattcatcc cgagtccaga cccactattg acagaccttc
     4561 agcataagct ttccgatatt caactgggta cgcaagatac atcacatatt catgagaaaa
     4621 tggctcctat tttgtcaaat aaagaaattt ttggtgtaga tctttaccaa gcagagcttg
     4681 gcagaaaaat agaagaaatg ttcatcaaga tggtagcagg accaaatgcc gtacgaaaaa
     4741 cattacagga ttacttacaa gaacatggag gaaatttatc atggaaatga cttttagatg
     4801 gtacggatca caggaagaaa agattacctt agatgaaatc agacagattc ctgcggtcaa
     4861 agggattgta ggcacattga tggatatacc agtaggagag gtatggccta gggagaggat
     4921 caaagaacta aaagaagaaa ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt
     4981 caatgtgcat gatgccatta aaattggatt accttcccgt gagcagtata tcgaaaacta
     5041 taagcagaca atccgaaact tggcagaata tggaatagaa gtcatctgct ataattttat
     5101 gcctgtattt gactgggtga aatctgacct tgactaccgc ttagaagacg gctcatccac
     5161 gttagctttt atatctgatg atattccaga tgatccaaga gaaatcgtcg cgcgcatcga
     5221 acattccagc aatgaatttg gattgccggg atgggaacct gagcgcttag ctcctatcaa
     5281 atccttgctt gaagcctatg catcagtaga cgaagaaaaa cttcgagaaa attttgccta
     5341 ttttctccaa tcaatcatcc caacatgtga agaggtaggg gtcaaaatgg ctgtacatcc
     5401 agatgatccg ccatactcga tttttggatt agctcgaatc gtaaagaatc gagaagattt
     5461 agattggttg tgtcgcatag tagacagccc ttataacgga atcactcttt gtacagggag
     5521 tattgcagaa gatccagaaa atgatgtata tgggatttta gcagaatttt gccaaagaga
     5581 tcgaatccca tttgcacatg ttcgaaatat taaattttta gctaatcgag atttctatga
     5641 ggcaccacat aaatccgaat acggttcatt agacatgtac cgcatactaa aaacaatgta
     5701 tgacaacgga tttaacggat acatccgtcc tgatcatgga agaatgatct ggaatgagac
     5761 aggaagacca ggatacggct tatatgatcg agcattaggt gctgtttatc taaatggatt
     5821 atgggaagca atcgaaaaag gaaacaaaag taaagataat tagtgaaaaa aactgtaatt
     5881 ttattatgta aataagaagg atgttcaaat tcttgataca taaatccgga ggttgttaat
     5941 aagcattcgg atttatgtat cttatttggt ctaggaagta acttgatttt cttttttcga
     6001 ttgatccatg agtgcttctt cttttaattc aaagatatat tctgagatcc tcataatcac
     6061 ttttttccag tcttctggtg tgatgattcc ttttaaaata tagatttttt tgatatgaga
     6121 aggcaattgt ttcaaagggc agtcgctgat aacgatgttg cagttctcca taaaatcatt
     6181 ttgtattttg atcgattcat ttgaaaaaat ttgttctatc ttaacgatca gatctttact
     6241 gatataaaaa tcacgcgtat agttcacacc aatcacaatt cgcggctgtt ttttgtagcg
     6301 atcatacaaa tgtactaggt cttcaaccat ccattgcaca ttttcctttt ctaaaataac
     6361 tggtaacaag tgttgatttt gttctctgaa atccatataa aattgttcaa tggcggtttg
     6421 gacttctttc aatacgctat gttcttgata aagcatgaaa gtaggaaggg gcgtatttgg
     6481 ttgattgaag ttaagaaatc cattataaag tttatttcgt gcgaatgcca atgagtaaag
     6541 aatcttttct ttttcaggta taaacaaatc aaatacagta caaagggact ccaccatttc
     6601 tcttgaataa agaacatgag gaacgtttgc tttttttaaa agtttgtatt gctccaagct
     6661 tgtttcttcc gattcgattt tcgtgataga aagtcgagct aaaatattca ctaagatgcg
     6721 ttgttcgcga caaattggca gattaggtcg caaaatatct acagagagat caataaaaaa
     6781 gtcaatatat tcatactctg tgaattcttt ttgtacgtct ttcacagaag ttgcaggaaa
     6841 attctcttta catattttta atagaaaata taatttatct atcataccgc tatttagtcg
     6901 gtcaagtact tcttttttaa aaaaagtatt catcataaaa gccactactt gcggatcttc
     6961 atcataaaat tctgtagcag tacttgaaaa gatgttccaa taaatatcta atgctgcata
     7021 ttgaatttgt acctcttttc cagaaaaaga ctttctgcca ttattagaaa aggagataga
     7081 caaaccgtac tatgctaaaa atgcattaat ctttttcatt ttcgtataca aataagaggc
     7141 actatagttt gtttgctgac tgaaactgat aatcgaattt gtctgacagc tgaacaaaga
     7201 agtcaagatc tgataactga ctgattcgtt gcagtatctt ccaaatagac gataatagca
     7261 gtaagccgga tcaattcctt caaaattcaa ctttaaaaaa ggtttttgct tatcaatatg
     7321 cagttctgtt agattgagac gctcaaagtc ttgttctaat tctttgatgt atagtaaaaa
     7381 atttgattca gacatatgga aatgatctgc taaatattgt gtagtaataa agaaacgatg
     7441 ttcagatagg aaaagaaaaa tatctaattt tgtattgctt tttttcttca agataaacgt
     7501 tttaatcgct tcttgagttt gcatgaaaga ggctcctttt tacttaaatt gttaaaactt
     7561 gaaaatatta ctgttttttt cataaattta gacaaaaaaa cagtaaacat ataggtagaa
     7621 tctctactat cttatttctt gcatacaaaa aaatcaagga gtgagagagt tatgagatat
     7681 tcaaccatct taatatcaac atttgcagcc ttaagcttat cgcttactat aaatcagaca
     7741 gtccacacag aggagacaga aacagccaaa tggacagaaa ccaagtgggc caacgtcaat
     7801 cgaattacct ttcaggatga attttcatcc ttcaattcag gagtcgcaaa attaagaaaa
     7861 ggacaaacag cgaccatcaa agcaaaaatt gactattcgg gagacttagc acctatacta
     7921 caatcagctg ttacatttga gaatgttgat cccactttgt cgattggcta tgacgagaac
     7981 actttgacta tccaacacaa gcaagcgata tttaccttca ccgttacatt aaatcaggac
     8041 ttgttaaaac cagcgctttt tactgtcaag gtatcagatg gactaccgaa cgatcatcat
     8101 ttaacgccgt atagtcagcg gcagacaatt gagcaagatt cagccatcga ctcaggagga
     8161 gatcttgttg aagaaccaac agataaacca gaaaatgaaa ataagccaga agtaccgcct
     8221 acagaaaatc cagacggaga acagaaaccg gaaatagaac ctggagaaga acccgataca
     8281 gaaactcaac cagagccaga caataaatca aaacctgaaa taacccctgg agaaaaacca
     8341 gatgtaactc cagaaccaga tacggattct ggaaaccaaa cagtaccgga aacaaatcca
     8401 gacacagata atgaaactga aaatccagaa aaaccagagg tagatccaga agaaaaacca
     8461 gatgtgactc cagaaccaga tacggacgca agagaccaag gaataccaga aaaaataaac
     8521 aaaaaaacaa ttcaagagga tggaaaaaaa gagagtaaaa aatcaaatct agcaattcta
     8581 aaaataaatg aagagcagct gaataaaaaa tcacgaatat ttgatagcgc ccaatcagct
     8641 gaaaccttaa aaagtagcaa ggatacaacc tttgctgtcc tgaaacgaaa aataaacagc
     8701 taccaaaatc cggagagtca caaaacaaag ttatactgtg gagcggaatc attctattaa
     8761 gtatagcaac aatgctttct gcaaaaagat ttaaacaaaa cagatctctc taacatttga
     8821 ataatgtctg tgaacgcatt tttcaaataa aaagaaatat ccagaaaatg accccaaaat
     8881 accagattga aatctgatat ttggggtcgt tcatacttta tctttcttca tatttatttt
     8941 aagggatatc tgtttctttt ggaagatggt tcgggatctt tttcactttc aatacatcta
     9001 aaaagaaggt aaagatcgga atgccgacaa tcaagcccca aacgccaaag aaccgttcgc
     9061 taacaagtaa gaccacaaat gtataaaaaa tcggcagttc tgttttgctg gacatcaact
     9121 tcggattcag cacataagac tcaattagat ggacaatcaa aatcgtaatc aggatataaa
     9181 caacatctcg gatgcctcct tgggaataag ctatgaaaga tagaggaata caagaaatga
     9241 taacacctgc tactgggatc agacttaaaa tgaaaatcat gatcgctaaa ctcagcagct
     9301 gatgaaagcc aaatacagcc aaggcgattg tagtgagcaa cgtatttaaa agagcgacaa
     9361 caaattgagc ttctaatacc aaaccaaatg tattgacaaa cttatcggca aaatagtaaa
     9421 tatcttgaaa gaaccaagaa aattcacttt ttaaaaacaa tcttgagaat acaattgttt
     9481 ttttcttttc aatcataaaa aagaaactta agatgaatga catagcaaac gccactgcca
     9541 tttttcctat atcctgtaaa tagccaagcg caatcgaagc gccattttga agctgttcaa
     9601 aaaaatcgtt tcgctcaata taagtatgga tatattgtaa aacaggatct gcatcttttg
     9661 gctgatgctc gtaaaaatta ataacggact ggatcatgtc aaatgtttga tgaataagca
     9721 tcgggatata ctttgtgaat gccagataga taaggtaaac gatcagtgta taaatcatga
     9781 tagctaaaac acctgtaggg attcggacaa atcgctgaat gatttgaaca agatgaatca
     9841 tcaaataagt aaagataaat gtgagcaaaa tggttgtaat catacttcta actaagaaaa
     9901 gaacaaaaac gacaaataat aaaacagtga gacggcgtat tttttcattc gccaaaaaac
     9961 gctgataaac ggtcaaatcg acaacctcca aataacaaaa aataaacacg ttattcatta
    10021 tatgatggaa tatgaaaata agaaagagca aaaagaaaaa ccgctaaatg acgttatctt
    10081 atgagttggt tttcctcata aaatcgttca tctagcagtt tcagcccttt ttctggtctt
    10141 gatgaaaatt atctagcatt tttcgggtca gatgttgcag tgtatccaat tcttcctgtt
    10201 caagtggaag ataagacttg atcttttgtg gaataccttc tgcttgtttt tgcaacgcag
    10261 caccttgagc agtcagcgta acatttacga ttctttcatc acccgtttca cgctgacgat
    10321 taatataatt tttttctgct aactttttta gtaagggagt cagcgtacca gaatccagac
    10381 acaaatcatt tcctagctct ttcactgaag tctttttctt ttcccataaa actaacatca
    10441 caagatactg ggtataggta agattcagcg gcttgaggag gggagtgtag tgtctaatga
    10501 tttctttaga ggcggcatat aaaggaaaac atacttgatt aactaaatat agatcttcca
    10561 tttttccacc tccgctgttt tactatagtg tatcatagcg gatttacaca aaaaagtcta
    10621 ttcactaact gctttcagag taacttctat atttccagct gtagcttttg aatacggaca
    10681 cactttatgc gctgcctcta ataattcttg cgtttcttcc agagaaagac cttcgataga
    10741 tccttcaatc tctacaccta agacaaagct cgtttcactt tctgaataaa gggaaacttg
    10801 tgcactgaca gtagaagccc cagagatatt tttttgctgt ttgatcaatt ctaatgcact
    10861 gttaaaacat gcactgaaac cagctgcaaa caattgttca gggttggttg cattttcgac
    10921 gcgtttacca ggagctgcaa tattcaattc gaatgtacga tcgggattat gcacttctcc
    10981 agagcgtccg cctgtgttga tcattttcgt agaatacatt tttttcataa agttaccacc
    11041 tttaattata ttttatacaa ttgaatttta cacaatataa ttgtggaaag caaagaaatg
    11101 gattggcata agataaataa gagaaagcac atgaaacaga cgcgctttct cgaatagcaa
    11161 acatatacaa aaaatatcag agacaaaaaa taatctcatc ataaacatta catatatttc
    11221 tcaaaaagtt gttttctttc ttcaaaaatt acttcacgat ggacctgatg aagcatccat
    11281 aaataaccaa aaggatctct gaacattgag ttagtcacac caaagtccat catttctgtc
    11341 atttcttgaa tctctacaca gccatttcgt aatgctttat tatgtgtgtc ctcgatatca
    11401 gagacgatga tattgaacca gatcgtattt ggatgttcag cagtaggagc agtcaaacca
    11461 agagacgtgt tttcatccag catatggaat ctcgtaccat acaaggtaaa aatgacttca
    11521 ttattgcctc tagggtaatc tgttgcctca atcagttgta tttcatcaaa gattgtttga
    11581 tacagttcta gtgctttcaa actatcattt aatacaaaat cgatttccgt tcctttatgc
    11641 ataaagatct cctctccttt tcagtcgaac agttactaga ttcttttatt ataccagcta
    11701 aagaaaacgc attcaaaaaa atagcggatt tacttaccaa ctcattcgat tggatgataa
    11761 gtaaatccgc gtcccattac ttctgctacg ctgctgacaa tgacaaaggc acttggatcg
    11821 atttcttcta tcaatggttt cattcgggaa aattcatgat cagtcacaac gatcaataaa
    11881 acctcttttg actttcgatt atacccgcct tttgcctcta gcagggtaag gccacgtcca
    11941 atctcagcac ttaagcgaag gcggatgtct tccaaataat gttcactcat gaccataaca
    12001 gatttctttc gattgatacc tgtttcgata taagtcatga caattgaagt gattgcaata
    12061 gataaaatag cataaagaaa ctcttctatt ccaaaaataa acagattaag agaaacaacg
    12121 aatgcgtctg taaaaaataa gccaatcgaa gtattcaagt ggaaatattt tttaaaaatg
    12181 aggggaggga tcgtcgtacc gccgctagaa gagttgtttt tatataaaat cgcaactcct
    12241 aaagcaaaaa tcgcacttcc aaaaatgaca gacaacaacc gatctgaagt aatcattgtt
    12301 tctggaacta ttgcaatgat taaaggaaaa accaagcttc cgaaaagtac tttaaaaaag
    12361 ggttttttac ctaaaaatag aagagctagt accaacataa cgatattcaa cactaaaata
    12421 actatagctc ggtcaaagcc taaagcagct tccagtaata ttcctagacc gctgacacct
    12481 ccagcagcaa tatgatgagg ccctaaaaac atattgatag cgactcctaa tatgaagagg
    12541 gcaaaaataa tcttaatgta tgtccatata aattgtctcg tttctttcat ttattcactt
    12601 ccttaattca ttaataatac tcttatctta ttataactta cttttcacct taagttaact
    12661 ttaattaata aaaaaattag gctagtctaa attattatgt ttcattcttc tagaatatac
    12721 ttctacgcca aaaactgtta aaaaactttt actttagaaa attggagaaa ttgatcgctt
    12781 aagataaatt caggacacta aatagtacag gaagtagact aaaactttta aaagttttaa
    12841 gtctatctat ttggcaagtt taaaaaataa aaattcggta tcaaaaaact gttaccgata
    12901 acatttttga aagcgttgtc ttttgcttct ctatcactta tagtgatgac gtcatattaa
    12961 aacacaagga ggaaatcaat ggatacagct gcaatttatc atcgaccgga aagtgaatat
    13021 gcatttctat atacgaatac aagatttcgt attcggcttc gtactaggaa aaatgatatc
    13081 aaaaaagtat atgtgctatg tggagatcca tataccataa caactgaaaa atggtatcaa
    13141 aaacaacgac caatgaaaaa atgtctaagt accaatgttc atgattattg ggaaattgaa
    13201 gtgacgagcg aaacaagtcg cttacagtat gcgttccatg ttgttgggga agataacacg
    13261 gactgttttt acggtgatca aggaattttt ccttatcgag aaaatgtgat tactgaacca
    13321 aatttttatt ttcgaatacc ttattttcat caaatcgatc gattcactat tccagaatgg
    13381 gtaaaagaaa cagtatggta tcaaattttt cccgaaagat ttgcaaacgg ggacaaagca
    13441 aatgaccccg aacacacttt accttgggga agtaaagatc cagaccgaga agactttttc
    13501 gggggagatt tgcaaggtgt gatggatcat ttggattact tgacggacct tggcatcaat
    13561 ggcatctatt tttgtcccat atttaaagcc acatccaatc ataaatacga tacgattgat
    13621 tattatgagg tggatcccgc ttttggcgat aaaaaacttt taaaaaactt gattgatgaa
    13681 gcacataaaa gagggatacg tatcatgttc gatgctgtat tcaatcatat gggtgtccat
    13741 tcaccacagt ggcaagacgt tctcaaaaac ggagaaaaat cgatctataa agactggttc
    13801 cacatccgtt ttttcccagt agattcttat cagatgactg acttaccaga aactgctgaa
    13861 aatatcccat atgatacttt tgcttttact ccattcatgc cgaaactaaa tacagcaaat
    13921 ccagaagtgc aaaaatattt gctggatatc gcaacttatt ggataaaaga attcgatatt
    13981 gatggatggc gtcttgatgt tgctaatgaa gtcgatcatc atttttggaa aaaattcaga
    14041 gaagcagtta cagaaatcaa accagacatc tatatccttg gagagatttg gcattcctct
    14101 caagcttggc tgcaagggga tgaattccat gcagtgatga attatgcatt tacagattcg
    14161 attaaagatt attttgcaaa aaagaaaatc acagcttcac aaatggtcag tggaatgaat
    14221 catcagcaaa tgctttacag agatcaggtg aacgaaggaa cattcaactt attagattct
    14281 catgatactg ccaggatctt gaccctttgc caaggtaaca aagaattgat gaagtctgtg
    14341 atggccttta tgtttcttca aaaagggtct ccttgtatct attacgggac agaaataggg
    14401 atgactggtg aagatgaccc ggattgccgc aaatgtatga tatgggagaa ggaggaacaa
    14461 gatctagagc tattcggttt tataaaagaa cttgtttctc taaggaaaca gttgtctaag
    14521 attatttctg aaggttctac tcagtggcta atagtaaatg atcgtgaaga taagctctat
    14581 ttcacacgga aactagaggg acagatcatc tatgtttact ttaatcagtc aaaagaacct
    14641 tgggtagtag aacaggaaaa tgaggtgatt cttagtcaaa attgtcagtt gttagaagac
    14701 gggaaagcag agatacgaca atacggattt ttgatcatgt gttgaaaact gttaccggta
    14761 acaacaagaa agcgtttgca aatgaagatt tgtgcccatt ataataaggg tgtaacagca
    14821 cgttgaatac aaaggaggaa atgaagatga aaaaaagaag tgtgaaacag ttgggtttag
    14881 ggttaatctc tttaggaatc ttaggcggat tagcggcttg tggcaatggt aactcttcag
    14941 cagaagggga agccgatgaa ggaaaaacgt taacagtttc cgttgattct ggatataaag
    15001 attatataaa cgaaataaaa aacactttcg aaaaagaaaa cgatgtgaaa atcaagctag
    15061 tagaaaagga catgttcgat cagttggaat cattagcgtt agatgggcct gctggaaagg
    15121 gacctgacgt tatgatggca gcatatgacc ggatcggtgc gttaggacag caaggacatc
    15181 tggcggaagt aaaactggga aatgaagcag cttacgatga aacagacaaa gcccaagtga
    15241 cttatgatgg aaaaatctac ggagaacctg cagtaatcga gacccttgtt ttgtactata
    15301 acaaagattt agtcgataca gcaccatcaa cgtttaaaga tttggagaat ctatccaaag
    15361 acagtcgttt tgcttttgag tcagaagcag gaaaaaatac aggattttta gctaaatgga
    15421 ctgactttta ttattcctat ggtttaattg caggttacgg tggctatgta tttggtgatg
    15481 atggcacaaa tccttcagat atcggtttga ataatgcagg tgctgtagaa ggaatctctt
    15541 atgcaacaga ttggttcaaa aatgtttggc caaagggaat gcaagacata aaaagtgcag
    15601 gagacttcgc cagccagcaa ttcatgtcca ataaaactgc agcaatcatt gacggtccat
    15661 ggcaagcgca aacatacaaa gaaaacaata tcaattatgg tgtagccaaa attcctactc
    15721 taaataatgg acagccttat caaccgtttg gcggaggaaa aggatgggta gtcagcaatt
    15781 atgctaaaaa caaagacctc tctcaaaaat ggcttgatta tgtaacgaat caggaaaatc
    15841 aagaaaaatt ctttgaaatg gtaaatgaaa tccctgcaaa ccaacaagct agggaaacag
    15901 caaaggctaa aaacgatgaa ttaacaacag ctgtgattga acaatatgaa acagctcaag
    15961 cgatgccaaa catcccagaa atgggcgaag tttggacagg tgcagaaaac ttgatgttcg
    16021 atgcagcatc aggttcaaaa acaccaaaag aatccgcaga tgaagcagta aaaacaatca
    16081 gtgaagccat agaacaaaaa tactgattaa aatgataacc gtccattttt ataaaaaatg
    16141 gaacaggtgc ttcagataaa agtggcaaga gcgagatgga aaattatctt gctcttgttg
    16201 ccattatctt aaaagcagtt tgcggtttag caagtttaga gggggcattc agatgcagca
    16261 agataaaaca agcaatatca acgaaaaaaa aatcacgctt tgttcactta ttccagggat
    16321 ggggcagttt gtaaatggcc aagcttttaa aggtatcgtt tttttcgcca tattcgtcct
    16381 attcattggt cagatgcttc tgggcggatt tcatgcattg gtaggattgg ttactttggg
    16441 aagtatacca atggaagatc attcattatt tattttgatt gaaggcactt tgcaattcat
    16501 cattacaatc attttcttga ttttttacgg tttgaatctt ttagacgctt atcgagtagc
    16561 gaagatgcgt caagagggca aaaaaatcaa tcggacgatg aaggaagtca tacataatat
    16621 aggtgatgaa ggttttcctt atctattgac acttcccgct tatcttctca tgatttttac
    16681 gatcattttc ccagtcctag ttacattgtt cactgcattt actaattatg acttcaatca
    16741 tattccacca gccagtttaa ttgattgggt aggggctaaa aactttttta gtatcttctt
    16801 tttaagctca tatcgtaata ctttttttgc agtcttttcg tggaccgtga tttggacttt
    16861 atgtgcaaca actttgcaaa tcacccttgg tattctcaca gcggttgttg ctcaccagaa
    16921 atttattaaa ttcaaacgta ttttcggtat cattttctta ttaccatggg cggtcccagc
    16981 ttttattact atcatgagtt tttcgaatat attcaacgat agcgtaggtg ccatcaatac
    17041 tcaagtgatt cctttcataa accagcttcc ttttgtagat atcagtgcaa tctcttggaa
    17101 aacggacccg aactggacga aagtagcaat tatcttagtg cagggctggt taggatttcc
    17161 ctatatctat gtgatggtta caggaatctt acaatcgatt tcagaagact tatatgaagc
    17221 agcaaaaatc gacggagcaa atgcatggca gcgcttttca aacatcacgc ttcctgctgt
    17281 cttcctagtg gcagcgccaa catttattac acagtatacg gggaatttca ataatttctc
    17341 catgatctat ctgtttaaca atggcggtcc cggaagcgta ggcggaggag ctggttctac
    17401 tgatattctt atttcctgga tctataaact tacgactgga acttctccgc agtattctat
    17461 ggcttcagca atcacgttga ttatctccgt catcgttatc tgtgtgtcat tgctggtatt
    17521 taaaaagaca aatgcgttca atatggggga ttaacaatga aaaaatttca ttcgagtgct
    17581 cgattccaaa aacgactttc tcactttttt acttactttt ttcttttaat actgtcagtc
    17641 attatcatct atccgctttt gatcacagca agtactgcct tcaagtcagg aaatattgca
    17701 gcttttagtt tagatctttc aagtgactgg actttaggaa actttactcg gctattcagt
    17761 gaaacacttt acgggacttg gtataaaaat acattgatca tttctgtcct aacaatgatc
    17821 gtacaagtga ctgtagtaac tttggcgggc tatacttata gtcgctatcg ttttatcggc
    17881 cgcaaatcaa gtctgatgtt ctttttagtg attcagatgg taccgactat ggcagcattg
    17941 actgcctttt atgtcatggc tttcttatta gaagcgctcg ataagtactg gttcttgaca
    18001 atgatctata taggcggtgg aattccaatg aatacttggc tgatgaaagg atactttgac
    18061 actgtaccaa tagatttaga tgaatctgct aaacttgatg gggcagggca tttcaggatt
    18121 tttttccaga ttgtattacc gcttgtcaaa ccgatgctcg ccgttcaagc attatgggct
    18181 tttatgggac cttttgggga tttcatgtta gctaagttcc tgcttcgaac acctgaaaat
    18241 cagactgttg ctgtcggctt acaaacattt atttctgatg tgacaaacca aagagtctct
    18301 ctttttgctg ctggggcaat tttgattgca ctaccgattt gtctattatt cttcttttta
    18361 caaaagaact ttgttaccgg cctgacatct ggcggaacaa aaggatagga gcagagtatc
    18421 atgcaaacat ttccgttaaa ctatttttcc gcattacgta cgccaactca agtgtttgcg
    18481 ggacgcaaac tattatcatg gcctaaattt ttcttgatat ttgtattttt agtttccttg
    18541 atggtcatgc ctgtgactct tttttatgcc aatcagatcc aagcgattcc attagaacaa
    18601 tttctatccg ttcattcgct gatcgatgaa caaggaaccc aaaaattttc tgaacttgaa
    18661 ttatctgaaa ctgggttaca agcatcgcaa caaacgatag ctgtgacccc tgaaattttg
    18721 gtaggtgtgt ctttatctga gaaacaacaa tccgatcatg gaacttttat tgattttgag
    18781 aaggaacaat gggtgatcca acaaaaggat aaatctggta tacgccgcta tacgatgaat
    18841 tatagcccat ctttccaacc agattctgta cgtacgcccg aagatttcca acgattttta
    18901 gaaagagaat tttatgccag taatcgtcca acaatcattt tatcttatag tttaagtctt
    18961 ggtcttgtcc tatttgtcat gacgagtctc attttattcg gggcttcctt tttcttgtgg
    19021 atgacaagaa aaagccaact ttcatccatt catacattca aagagagtgc aaatttgatg
    19081 ctgaatgtta tgggaatagg ttccatggca gcagcagttg tagggtttat ccactttgac
    19141 tttatcttga tgctaagtgt ccaaacaacg ataactgtct tgctgctttt atgggttttt
    19201 gtcaaaacaa gattcaaaga taagcgtgtc gaatgacaag tttaagcgat ttatgcgaca
    19261 atgaatgtga aaaaaatgaa aaataaagta gtttttgaaa aatttctgaa aaaattaaac
    19321 ttcttagttg attcttttct tttttagtat taacgtaata gggtagtttt tttaaggagg
    19381 aaaatcatgt tagagaaaac tttatacaat caattatttt cacgttcttt ctcgctccct
    19441 gtcgaagtaa attattggga cggtacaacg aaacaatacg gagatacgga cactcctccc
    19501 caaatcaaaa tcaatatcca tgaagaaatc ccaatgaaag agattacaag taacgcatct
    19561 ttggcactag gagaagccta tatggatcat cgaatcgaga tcgagggaag tatccaagca
    19621 ttgatcaacg atgcttatca gcaagcaaac agctttttac gaggaaaaga ttatctaaaa
    19681 tggctgccaa agaaagaaaa acatacgaaa caaaaaaaca aagaagatat ccatgcgcat
    19741 tatgacttag gtaacgactt ttataaactt tggctagatc caactttgac ttattcttgt
    19801 gcttatttca atacacctga agatacgttg gaacaagcac aaatcaataa agtccatcat
    19861 atcttagaca aactctttat caaagaaggc gaaacgttgc ttgatatcgg ttgtggctgg
    19921 ggaacattga tgttcactgc taccaaagag tataatgtca aggcaactgg gatcacacta
    19981 agtgaagaac aatacgattt gattaccaaa agaataaaag aagaacattt agaagataaa
    20041 tgccgtgtat tattaatgga ttaccgcgaa ctaaaaggcg aaacgtttga tcatatcaca
    20101 agtgtaggta tgttcgaaca tgtaggttct gaaaatcttg aaggttattt caaagtggta
    20161 aaagatttat tgaaaccaaa aggaacagct ttgatccatg gaatcagccg acaacaaggc
    20221 ggtgcgacaa atgcttggat caataaatat attttccctg gcggttatgt ccctggtgtt
    20281 actgaattag ttgataatat tacaaaaaac gatttgcaac tgattgattt ggaaagctta
    20341 cgtcgtgact accaattgac attagaacat tggacaaaga attttcatca agtccaagat
    20401 caagtcacga aagaaatggg tgattccttc tatcgaatgt gggatctata tttacaagct
    20461 tgtgcagctt catttgaatc aagtaatatc gatgtgattc aatatttgct tgttcaacct
    20521 ggaaacaatg acatcccaat gcatcgtgca taattgaggc aaaagtactg tttaaagact
    20581 atttaaaaag aagctaagtt ttatgatgct ccagaagtga acttttttgt ctaacttcta
    20641 ggattgtctc taaagcttag tttctttttc tttatatttt cataataaaa gtttggttct
    20701 ttaattttta aacatgacag aaaatttttt cttcgttata cttgtaatag aatactattt
    20761 tataacaaag gggatcgaat caatgaaaaa aactgttatt tatactagtt tatttgcagc
    20821 tttgttttta gctgcttgtc aatcaaatac caatcaaggc acatctggga atacgtcttc
    20881 cagtagcgaa cagcgctctt cacaggtatc ggactcctca acaaaagcaa gcacatcttc
    20941 gtctagccag acaaaaagtt ctagtgaagc aagtactcag tcagatatca aagtgtctgc
    21001 agctcgtgcg atcgaattgt tccaagataa atacccagat actgcaatta ctagtttgga
    21061 attagatagt gattggggat cttacttcta caagatagaa ggtgtcgatg atcaaaaaga
    21121 atacgaagca agaattgatg ctcaaaatga aaaaatggag acagagaatc cagaaactct
    21181 agatcgagat gaacaaaatg gtcaaaagaa aaaagaagat ggtctggatg ttgataaatt
    21241 gatcacgatt gaggaagcag caaaaatcgc cgtcgacaaa gtaggaagcg gtacggcaac
    21301 agattgggat ttggataaag atttaggtac tacttattgg gaagtcaaag taaaagacgg
    21361 caatcaaagt acatcagtca agatcaatag ccaaacaggc gaagtactgt caactgaaca
    21421 agatgattaa gaatttcttc ggaaaacaag agttgatttt tcttttagtc atgatacact
    21481 agacaaaata acttgtttga aggagcgatt tacttgagtt tagaaacgac ctttgccaat
    21541 catatctttg ccaatccttt gatgaatgca tctggtgtcc actgcatgac tacccaggaa
    21601 ttagatgaac tggctcattc agaagcagga gcatttatca ctaagagctg tacgatcaat
    21661 gaacgaaaag gaaatcccga accacgctat tttgatgttc ctttagggag tatcaattct
    21721 atgggacttc caaacttagg tttttcttat tatctagagt atgctcttgc gtatgaaaaa
    21781 gtgcaggaaa accaaaatca accgttattt ttttccattg ccgggatgag tgtccaggaa
    21841 aacttggaaa tgcttgagaa aatagaaaaa agtgggttta atgggatcac tgaattaaat
    21901 ttatcttgtc ccaatgtgcc tggaaaacca caactagctt acgattttga ggccacttat
    21961 gaaacactga aagaagtatt cagcatcttt tctaagccgc taggaatcaa attgcctcct
    22021 tattttgatt ttgctcattt tgatcagatg gctgatatct tgaatcagtt tccattgacc
    22081 tatgtcaatg ctatcaacag tgtgggcaat ggtttgtata tcgatacaga acaagaagca
    22141 gtagtcatca aaccaaaaga agggttcggc ggcattggcg gagagtatat caagccgact
    22201 gctttggcga atgttcgcgc tttttataca cgtttgaaac cagaaatcca aatcattggt
    22261 acaggtggga ttcggacagg acaagatgca tttgaacatc tgctatgtgg cgcaagcatg
    22321 ttacagattg gtaccgagct tcataaagaa gggccggaaa ttttttctag aatcataaaa
    22381 gaattaacgc agattatgtc agaaaaagga tatacgtcaa ttgacgaatt taaaggcaaa
    22441 ttacgcacca tttcatagca aagagacgaa ggtcatgatt gatcttcgtc tttttttata
    22501 aaaaacactc gtctgttcat tcctgtgcaa gcgtactcat tgtatgttag acgaacttgt
    22561 gggaaaataa cagaggcaaa aaggaggata atatgaatga tgcaacagaa ttagcaaaag
    22621 agcttaaaga aaaagaattt agttttcaag aactagttac ggatatacag aaaaaaataa
    22681 agaagcaaaa tcatgaattg aatgcgtttg tcgcgcttga accattcgag agtaattccc
    22741 acgagctatt gattccccag tcagaaaaag ggccattggc tggtatccca tttcctttaa
    22801 aaatgctcgg tcaagaaaaa aagggatggc tagctacttc aggatctcgt ttatttgaaa
    22861 cacaccgagc ttctcataca tctaattatg tgcggcaagc agaagcgata ggtctagttc
    22921 cttttggaca aaccaatgcg ccagagtttg gtttcaaaaa tattacagac ccagtcatct
    22981 atggcccagc aagaaaccct tggaaccttg aatgtacacc gggcggatca agtggaggag
    23041 ctgctgctgc agtttcatca ggaatcgttc ctttagctgg tgctagtgat ggcggtggtt
    23101 ctattagaat acccgcttcg ttttgcggat tgattgggct gaaaccttcg cgagggacga
    23161 tgccagttgg tccatatgct tggagaggat ggcaaggagc tgctattgat ttcggcttga
    23221 cagtttctat gagagacacg gaggctttat tttatgggat gagaagtatc cattccggtg
    23281 cgccatatca agtatcgcca gttgagtggc aaacacatcc aagaaaaaaa cggttgaaga
    23341 tagctttttg tgttgattca ccgatccact cacagattag tgaagaggca gtacttgctg
    23401 taaaaaaagc tgtagatttc cttggaaaag aagggcatga tttgacagag attccttacc
    23461 cactagatgg tcgtaaactg atcacgtctt attatcagat gaatggcgca gaaacagcag
    23521 caatgatccg ctcgattgaa acaggcatcc agcgagctgt cagaaaagaa gaacttgaac
    23581 ccatttcttg gactttgttc caatatggtc agaagctctc tgccgctact tacatcgatt
    23641 ctctgcatgt atgggatgaa gcagcaattg tcatggaaaa cctacatcaa gaatacgatc
    23701 tattcctttc accaacgact gcatttaccg cccctaaaat cagcgaagat ttgcaaagtg
    23761 atgcgataag agcacagatg gaacacatca gtgattgttc tgaagtagaa ggtttagacg
    23821 ttgtttcttc catgtttgag gagagcttga agatcacacc ttatacacag ttggctaatc
    23881 tgactggaca accagcaatc agcttgccta ctcatttgac aaaagaaaac atgcctctag
    23941 gaatccaatt tatggctgcc agaggaagag aagatttgct atttcaaatc ggctatctat
    24001 ttgaagcaaa tcatcagttc catttaccaa atccatggaa atagcagaaa caaggagaag
    24061 aagaatgaag aaaaaaagat tgtttgctct aataactagt gcctttttta ttttaagcgc
    24121 ttgcggtaca gctgccgcta atcaggaaga taacagctgg agtaatgtag aaaaaaagaa
    24181 agaattagta gtagcgactt ctggcacact gtttcccgca tcttattata atgatcaaaa
    24241 tgaattagtt ggttatgatg tagatttggc aaaagaggtg gctaaacggt taaatgtcaa
    24301 aatctccttt aaagaataca acgtggatgg acaaatcact tctgtttcaa aaagagaatc
    24361 agattttgct gcgaacgatt ttggtatcac aaaagaaaga gaaaaaaaat tctccctttc
    24421 agagactttc aaatattcat ttgacagtat gatcgtacga aaaaaagatg attcaggaat
    24481 ccactcactt gaagatctta aagggaaaaa agcagctggc gaacccaata ctagctatat
    24541 gcgtttagcg gaaaaacacg gagctaaatt agtcacatat gacaatgcca cgaatgatca
    24601 gtatttaagc gatgtagcaa atggtcgaac agatgttatt ctaaatgatt attatctgca
    24661 gaaaatggcc gtatctgctt taccagatat ccctgtaaaa atactggaga acgtttattt
    24721 caatgaaaac gaaagtggtt ttttattgaa agaaggaaat gatgctctga aaaagaaaat
    24781 cgatggcgta ttaaaagaga tgaaagaaga tgggacgaca aagaaaatat ctgaaaaata
    24841 ttttcaaact gatgtttcag tcaaaccgaa agaaacgatg gctgagtcgg tgagtgagtg
    24901 atgtactttc ctctaattga tttttccctc atgtggactt cacttccttt cgtactgcaa
    24961 ggcttggtat acacattagg gattggcttc gtgtcgtttc ttttaggaaa tctggtaggg
    25021 ttgcttttga cagttttagg tcttttggat tggctgcctc tgaatgtatt tattcgtttt
    25081 tatttatcgt tctttagagg cattcccgca ctagtcctgc tgtttctgtt atattttggg
    25141 ttaccttatc aattaagtgc cttaacggct tcagtcatct gctttacgat taccagcagt
    25201 gcatttattg gtgaaatcta tcgaggatca ctagcaggcg tttcttcagg tcagtgggac
    25261 gctggtttag cttctggttt ttctttcttt ggggtattga aatatatcgt tttgccgcaa
    25321 gcttttcgaa tctcagtacc ggcactcagt aacgtagcta tggatctagt gaaaggtact
    25381 tcgttagcag caacaatcac agtgccagaa atatttcaaa aagcaaagat cataggcggt
    25441 cggacattcg attatttttc tctctatatt ttagttgcac tgatttattg ggtcatttgt
    25501 atcgggatcg aaaaagtgca acagtattta gaaaaacgtt ctatgcatca gtcaatctga
    25561 aaaatagaaa tggtgctagc gtggctggca ccatttctat tttttgtttc ttgtatattc
    25621 tgcctagtat tttaaaacca tttcatgtaa aatgtaaatc gaaaatatgt tcggaggtag
    25681 aaaacatgaa gaccccgcta accatcgaaa tagaatcctc gttataccaa tattgcatcg
    25741 agcaaggcgg cttggttgta gaagaagtca cgatgcctga tgaccaagga attgtagata
    25801 cgcttagctg cttgacaaaa tttgatggaa gccgagaatg gcgatgctat gagctgaaag
    25861 tgacaaaagc agattttcgc tctacagcca aactgtcatt tgttggacat tataattatt
    25921 ttgttttacc aagtaaacta tatgaagaag taaaaactga aatccctaca ggaataggtg
    25981 ttttagtgta tcgtccgtac acggttgaag aggagatgcc tgcctctggt acatttctga
    26041 ttgcgaaaaa agcagcaaag aaagagcttg ctgtaccgga agaagctttg acaaaccgct
    26101 tcatggcttc tttattcagg gaagtaagaa aagcaaagca aatggagcga ggcgtaaaat
    26161 attttccaac agatcaattg tacaaagaat tgaaaagacg ttcagaaaat caagatccat
    26221 tttctaacga acgatattat gaacggtttt tagaggaaat agctagtcag gcaatcaatg
    26281 agttgcaaga agaagttatc gcgcttcagc atgattatga atatttgagg caacaacagc
    26341 agatccgtcg gttgcctaca gagccattgg agtgaaaaat atgtactatc cttatttaag
    26401 aggaaaacaa tttgacctat tagcattaaa agaagcactg tcgcgaggat tgctctccaa
    26461 taaaatccaa ccagtgatcg aaccagtacg tgattccgct acgcttaaaa atgtgatcga
    26521 attatttcaa aaaaagcacc atcgaatcgc tgtgatccaa aatccccaag taggacaatt
    26581 caaattattc gatcagcatg tgcacagttg gacagtcaaa gaaaatagct ctgtcgtccc
    26641 tgcacagatc ttaacgccag aaaatctcag tgaagtactg gattcacctc cagcgtttct
    26701 tgttttcgat ggacagcact acccaaaaga tacagaggtg tggaaacagt tggcaggagt
    26761 tgattcgaaa tttttgatac cagatactag tcgctttcgc atctggctgc ccgaaaacaa
    26821 aattgtgatc cgtgactctt ttcaaacgag aaagcatgtt gaaagttatg cagataaaac
    26881 agatgacttt tttagtgatg actatctttt tttccacgca gacggatata ttggattttc
    26941 tgatttcacg atcgaaggta gccgttattt cgataaaggg ttccctagcc gagcgcttgc
    27001 tatccatcta acgtatattg atgcttacgg caatatacgc gtgaaacatt ttgtctctga
    27061 cagtaacgac tctgctaaag accaagcatt gaagtttctt gaagcaggag ataaaatgaa
    27121 aaaatggatc atgcgtcacc atcatcaact cttgattact tcaggaatga ttgaattatt
    27181 ggctctttac cagcaacaaa aattccctgg actgggagtg ttgaaaaagt ggtcacttat
    27241 gcatcatttg gaacttatta gtcacctgct agatcatcca aaagattggc ttaaatatta
    27301 ttcaaaagtt caggaactat atgaagtgat ccaatgatta gataatgtaa atgaaaaagg
    27361 aatagaaaag aggaaactgg atgtatattc ggcaaacaag gaaagaagaa gtagaagaag
    27421 tggtagaaat cattaatgcc gcagcggatt tcattgcttc gcaaggctct cctcaatggc
    27481 aaaacgggca gaaaccaaca aaagaaacga ttttgaacga tattgaaaga aaagaaagct
    27541 atgtattaat agtggaagat caaattgctg gaacagctgc attagtctca agcgtggatc
    27601 ccgtttacac aaaaatagat ggtagttgga aagaatcaac tggtccttat ttatcgattc
    27661 atcgtgtcgc tttagcagaa agtactcgag gaaaaaaact agcaaaaccg ttcttagaac
    27721 atttgttgac gattgcagcc agcaaaaata tccaagatat ccgtatcgat acttatccag
    27781 ctaatgaacc gatggaaaag accatctatt ctgctggatt cacttatcaa gggatgattg
    27841 aatttccttt tgaacacggc gaaagaaaag catatcaaat aatcatcaat tgactataaa
    27901 atcatctgag caaagataaa gacattaaca ctactgaaaa aaacaatcat atgatagcca
    27961 agaggttgta acagaagtgg ccagcttcaa aaaacaagaa agaatttccg aaaatgcttc
    28021 tcaaattttt gtggaatttt agcgtatttt acgaaatagc tgctttctga aactcccttc
    28081 atcgagaggt cgtgacatag cttttgtcac gacctctcgg cttatcctac gagtattatt
    28141 cgatacattt atatcaataa tccttttttt attttatctg cagcttcttg gaacgctcca
    28201 tcgtttgaca cgactaaaag tgaggggata gctttcaaac gtttcaactg tgctaaatct
    28261 tctacataaa ccgccagtct ggctgaaatc tgttcagatg aatctccacg ttttttcagg
    28321 cgcgattgca atgtttcttt agagatcgta ataaagatag gacggactac cgtcccgaaa
    28381 tactcatata aatgccaaaa accatttgct gtcaaaacgg cgtaacaatt tcctactgtc
    28441 agtttctttt taatttctgc tttactggaa ccatatttat gttgagcata ttggtcccac
    28501 tctgcaaact catttccctt gatcatctga ttgaatgtat tgtcagaaac aaaataataa
    28561 tcctcttgat ctttttcccc aggtcttggt gatcgattcg tgtaagtaat gatcttttgt
    28621 tccggctgaa aaatagcttc tgcgagtgaa gtttttccag aaccagaagg accgataaat
    28681 acatagcaat aattcattgt catttctcct ttgatgattg aacaagtgaa aaagctttgg
    28741 aaaatatttc caaagctttt aaaagtaact ttgctttttt gtatattata ccagcaagcc
    28801 agtcacaata gcacttaaaa agctgactaa agtcgctgtt agcaaaattt tcaatccgtg
    28861 ttttgcgaca agatttcctt tttcttcatt taatcccttc atcgcaccag aaatgatccc
    28921 gatagaagag aagttggcaa atgaaacgag gaagacagat ataattgctg ttgtacgctc
    28981 agtaaaatgg aaattaccat ttgctaattc tgtcatagca acaaattcat ttgtaaccag
    29041 tttagtcgcc atgatactac cggcatccac tgcttctttc catgggattc cgctgatgaa
    29101 ggcaagaggg gcaaagatgt aacctaggat ctcttggaaa gtgatgccga atatcccgtg
    29161 gaagatagca ttgatcatgg caatcaaagc aacaaaaccg ataagcatag cagcaaccgt
    29221 gatcgccaca tggaaaccat caagaatgta ttctcccaac atttggaaaa atgtctgttc
    29281 tttgttttct tcaatgatca gctcatcttc tttttcttct aatacatagg gatttacaat
    29341 tgaagccaga atataaccgc caaataagtt caaaacaagg gcagtgatca catatttaga
    29401 atcgatcagt gccatataag atccgacgat cgacatagaa actgtagaca tagccgatac
    29461 actcatggtg aacaaacgtt tctcagatag gaagggtaat tcctttttta tggaaataaa
    29521 aacttccgat tgtcctaaaa tagcagaagc tactccatta taagattcca atttgcccat
    29581 cccgttgatt ttacttaatc ctagaccaac agctttcata aataatggaa gaatcttgat
    29641 atagcgtaga ataccgataa tagcagaaat aaatacaatt ggcatcaata cggaaagaaa
    29701 gaaaggtgca cttcctttat ttgctacacc ggcaaaaaca aagtcaacac cttgcccagc
    29761 aaaagcaaga agatgatcaa acatttttgc caatacagaa acaaccgctg taccaaaagt
    29821 tgtacgtaat aatacgaaac caagaacaag ctgtaaaacg aacatcacaa ttagtggttt
    29881 caatttgatt gcttttttgt tactgctgat caaaaaagca agccctaatg tgacaaataa
    29941 accaataata cctaataaat aactcaaata atcactccta ttccaaattc cgttttattt
    30001 tacctgtttt gcaaataaaa tactaatctt tagtgaaatt ttcattactt ttttagagtg
    30061 aaacgcattc attttccatt agaaaataaa gcgaacaaaa t
//
LOCUS       DAYYCW010000021        29946 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000021 DAYYCW010000000
VERSION     DAYYCW010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.021"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..2424)
                     /locus_tag="ACHAYA_000922"
     CDS             complement(61..2424)
                     /locus_tag="ACHAYA_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFM8604744.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2589..3093
                     /locus_tag="ACHAYA_000923"
                     /pseudo
     CDS             2589..3093
                     /locus_tag="ACHAYA_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="ACHAYA_000924"
     CDS             complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="ACHAYA_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM8604745.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3575..5881)
                     /gene="infB"
                     /locus_tag="ACHAYA_000925"
     CDS             complement(3575..5881)
                     /gene="infB"
                     /locus_tag="ACHAYA_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM8604746.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(5894..6205)
                     /locus_tag="ACHAYA_000926"
     CDS             complement(5894..6205)
                     /locus_tag="ACHAYA_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM8604747.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(6202..6495)
                     /locus_tag="ACHAYA_000927"
     CDS             complement(6202..6495)
                     /locus_tag="ACHAYA_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM8604748.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="ACHAYA_000928"
     CDS             complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="ACHAYA_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM8604749.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="ACHAYA_000929"
     CDS             complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="ACHAYA_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM8604750.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(8333..12685)
                     /locus_tag="ACHAYA_000930"
     CDS             complement(8333..12685)
                     /locus_tag="ACHAYA_000930"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM8604751.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(12897..14606)
                     /locus_tag="ACHAYA_000931"
     CDS             complement(12897..14606)
                     /locus_tag="ACHAYA_000931"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM8604752.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="ACHAYA_000932"
     CDS             complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="ACHAYA_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM8604753.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(16103..16903)
                     /locus_tag="ACHAYA_000933"
     CDS             complement(16103..16903)
                     /locus_tag="ACHAYA_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM8604754.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(16900..17712)
                     /locus_tag="ACHAYA_000934"
     CDS             complement(16900..17712)
                     /locus_tag="ACHAYA_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM8604755.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(17907..18464)
                     /gene="frr"
                     /locus_tag="ACHAYA_000935"
     CDS             complement(17907..18464)
                     /gene="frr"
                     /locus_tag="ACHAYA_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM8604756.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(18467..19189)
                     /gene="pyrH"
                     /locus_tag="ACHAYA_000936"
     CDS             complement(18467..19189)
                     /gene="pyrH"
                     /locus_tag="ACHAYA_000936"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM8604757.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(19325..20206)
                     /gene="tsf"
                     /locus_tag="ACHAYA_000937"
     CDS             complement(19325..20206)
                     /gene="tsf"
                     /locus_tag="ACHAYA_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM8604758.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIEYDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="ACHAYA_000938"
     CDS             complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="ACHAYA_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM8604759.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            21446..21925
                     /locus_tag="ACHAYA_000939"
     CDS             21446..21925
                     /locus_tag="ACHAYA_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8604760.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            22142..23233
                     /gene="phnW"
                     /locus_tag="ACHAYA_000940"
                     /pseudo
     CDS             22142..23233
                     /gene="phnW"
                     /locus_tag="ACHAYA_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            23226..24011
                     /gene="phnX"
                     /locus_tag="ACHAYA_000941"
     CDS             23226..24011
                     /gene="phnX"
                     /locus_tag="ACHAYA_000941"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM8604761.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(24357..26048)
                     /gene="argS"
                     /locus_tag="ACHAYA_000942"
     CDS             complement(24357..26048)
                     /gene="argS"
                     /locus_tag="ACHAYA_000942"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM8604762.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="ACHAYA_000943"
     CDS             complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="ACHAYA_000943"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM8604763.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(27532..28551)
                     /gene="argF"
                     /locus_tag="ACHAYA_000944"
     CDS             complement(27532..28551)
                     /gene="argF"
                     /locus_tag="ACHAYA_000944"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM8604764.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="ACHAYA_000945"
     CDS             complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="ACHAYA_000945"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM8604765.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         7982 a         6445 c         5338 g        10181 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca
     3181 ggatttgcgg gattttttga ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg
     3241 ttcccataga caactgattc atcaagttca aagaacaatt caggtgtctt ataaatactt
     3301 agattgtgcc ctaactctcg acgaatcaat cctgatgctt tattcaatcc atcttgtgct
     3361 tttttcttat ctgatgctaa gtcagataaa atgctgtaat agatcgttgc ttgctgtaag
     3421 tcccccgtaa ctttcacatc tgtgatcgtt acgttctcca cacgagggtc acggaccttt
     3481 ttgcgcaaaa tatcattgac ttcttttaag atttcttgac cgactcggcg atcacggtag
     3541 ttagccataa tgattggcct ccttttatca gtttttagtc atttttgact tcttccatga
     3601 taaatccttc aattacgtca tctactttga tgtcattgaa tttttcaatc atcgcaccac
     3661 attcataacc cattttgact tctttcacgt catctttgaa tcgtttcaga cttgcaagtt
     3721 ggccttcata gattacgatt ccgtcacgga ttacacggac accgctatct ctacgaatat
     3781 atccttctgt tacataggca cctgcaatcg taccaacttt agaaactttg aaggtttcac
     3841 gaacgatcat ttgaccggtg attttttctt caaattcagg gtctagcatc cctttcatcg
     3901 cagtttcgat ttcttcgatg gctttataga tgatacggtg aagacgaata tcgacttctt
     3961 cagtatcagc ttgcactttt gcctgcggtg taggacgaac gttgaaaccg atgataatag
     4021 cgttgctggc tgctgctaag gttacatcac tttcattgat tgctccgaca gctgaatgga
     4081 cgatttttac acggacacct tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg
     4141 ctgaaccttg tacatctgct ttgatgatta cgttaacttc tttcaattcg ccttctttca
     4201 ggctttcaaa taagttgtcc aaagtcacac ggcttgtggc agcacggttt tccaataatg
     4261 cacgttttgc gcgttcttca cccgctgcac gtgctgtctt ttcgtcttcg aagactacga
     4321 aacggtcacc tgcttgaggt acatcattca agccagtgat ttctactggc gttgcaggtc
     4381 ctgcttcttt atcgcgacgg ccaagatcgt ttgtcattac acggacacgt ccgtaagtat
     4441 taccgacaac gattgggtca ccaacgtgaa gtgttccttc ttggacaagt aaggttgaca
     4501 caggaccttt acctttatca agacgtgctt cgatcactgt tccgattgcg cgttgtgttg
     4561 gatctgcttt taaatcttct acttctgcga ctaacaggat catttccagc aattcttcga
     4621 tgttttggcc gaatttcgct gagatctcaa caaaaatcgt ttctccaccc catgcttcag
     4681 gaattaattc atactcactt aattcttgca taacatgctg aggatttgca cctggtttat
     4741 caattttatt tacagcgacg atgattggaa cgcctgcagc tttggcatgg ttgattgctt
     4801 caactgtttg tggcatgacg ccatcatctg cagcaacaac taagatcgtg atatctgtga
     4861 tacttgctcc acgtgcacgc atacttgtaa aggccgcatg tcctggtgta tccaagaaag
     4921 tgattggttt accatcaata tcgatctggt aggcaccgat atgctgtgtg attccgccgg
     4981 cttcgccgct tgttacacgt gaatggcgca atgtatccaa taaggttgtt ttcccgtggt
     5041 ctacgtgtcc cataatcgtt actactggcg gacgtgagac taatttatct ggatcgactt
     5101 cttctgcctc gaagaattta tcgatatcag caatgtcaac ttggactttt tcttgtggtt
     5161 ccatgccata gtccgtcgct aacagttcaa tcgtatcttt atccagtgct tggttttgat
     5221 tgaccatgac accaagcata aataattttt taatgatttc tgctggctca cgatgaattt
     5281 ttttcgcaat atctgctacg ttcatacctt cagtatactc taatacatct ggcaattcac
     5341 ggaatttgcg tggtggtaca gctggtttag ttgattgttg ctgtttgcct tttttacctt
     5401 ttttattata acgattacgg ttattattat taaatttgcc gcgattgtta ttgtttggtt
     5461 tgttgttatt gttagtacgg ttttgattgc cgccttggct cgttccttga cgattttggc
     5521 tgctggtatt tcccgtatta ttttggtttg ttgttggttt ttttccttga ttcacttgac
     5581 cgctcccttg accgcgatct tgataactgc ggttattttt atttttttgt tgattgttcg
     5641 ttgtttcttt tttacgttga tcgttcggct gttgattggc tggttttctg cttgcttgtt
     5701 gtgcagcttg ttgtttcgtg cttgggtttt tgaacgcttg ttgcaatttc ttctcatcac
     5761 tgctagtaat cgcacccata tggtttttca catcgatgcc aagttgttgt gctttttcca
     5821 cgacgtcttt actagatttt ttgagttctt ttgctaattc atagattctt ttgttgccca
     5881 tgcaatcacc ttcctaacct tcaattagct ccttggtctt cttcgcaaat ccggcatcca
     5941 ttattccgaa taccttgcga ggttttccga tcatctgcgt gatttctgct tcagaaaata
     6001 gctcaaagca aggaacttca taatatgaac tcttatcttt tatctttttt cttgtattct
     6061 cacttgcatc ggttgccaca aacaccaatt ttgctttttg tttgcggatt tcagcaatgg
     6121 tcagttcttc acccgtgatt agttttcccg cgcgcatcgc tagaccgatg agattcatgg
     6181 ctttttgccg attaactgta ttcattttcc aaataactct ttacgtgctt tttggtgagt
     6241 gacatagtca agaagttctt gataaaactc atctgttaat gttgtttcga gtactcgatc
     6301 gagaattttt ttatcccaag cttgttgtac ttcttccggc tcaagtgaga catacgctcc
     6361 tcgtccaggc atttttccag ttggatcgat cgatacttcc ccttcttttg aacgggtgat
     6421 tcggaccatc tcttttttag gtttcatttc acctgaaacc acggatttgc gtaaaggaat
     6481 tttcctttgt ttcatctctt tgcctcctta ctaaagttaa gcttctggtt tttcttcgac
     6541 cgtttcattg ttgaatgcaa ttgtttcata ttcatcgtct gttaaatctg actgaacgat
     6601 tgcttcatca tgcagttctt ctgtttcttt ttgttgttct tgattttcat aaaattccgt
     6661 catgtcagat tctgatttga tatcgatttt atggttagtc aatttagcag ctaaacgtgc
     6721 attttgtccg cgtttaccga ttgctagcga taattggtag tctggaacga caactgtgca
     6781 ggcctttgga tttgtctcat cgaagatcac atccatgact tgtgcaggat tcaatgcatt
     6841 tgcgatgaat acagctggat cttcattcca ttcgacgata tccatgtttt cgccttttaa
     6901 ttcattgacg attgcttgaa ctcgttgtcc ctttggcccg acacatgtac cgactgcatc
     6961 gatatttgga tcatttgagc gaactgccac ttttgaacga tcgccagctt cacgggcgat
     7021 gctgacaatt tcaacgattc catcatagac ttctggtact tcttgttcaa ataagcgacg
     7081 caataggtct ggatggctgc ggctaacaaa tacttgcgga cctttagacg tattttctac
     7141 tcgggaaaca taaactttga ttcgatcatg tggttgataa aattcatttg gcatttgatc
     7201 ttgtttagaa agaactgcct caattttacc aagattgaca taaatataac gtttatcctg
     7261 gcgttcgacg attccttgca tgatgtcttt ttcataagca ctgaattcgt tataaataat
     7321 cgttctttct gcttcacgta cacgctgtaa aataacttgt ttagccgttt gcgcagcgat
     7381 ccgtccaaaa tcttttggtg ttacttcaaa acggatcgta tcaccgatct catatgctgg
     7441 attgattaaa agtgcatctt tcaatgagac ttccaactgt gaatccatca cttcttccgt
     7501 tacttctttt accgcataga catggatctt tccttttttc tgatcaaatt ccacttcgac
     7561 gttttgtgct tggccatagt gacgtttgta tgcagaaacc aatgctgctt ctaaagcatc
     7621 gatcacgatt tcttttgaga tccctttttc agcttctaaa gcgtctaacg catttaacat
     7681 ttctttactc atttttttct gattcctctc cgtgattaaa attggatagc taagcgagct
     7741 tttgcaatat tttttcggtc aaaagtcaat tctttttctc gtgtttttat gcgtattttc
     7801 aatgtaagct gttcagcatc aaatgattga agaaatcctt catattgttt ttcgccatct
     7861 actggttggt ataaggagac atggacataa tcaccaagtg ccttttcata gtcgctttct
     7921 tttttcaatg gccgctctgc tcccggggag gatacctcca ggaaataagc ttgaggaatc
     7981 ggatctggat ctgctgcatc aagtttttca ctcagtttct cactgacaaa ggcgcattct
     8041 tcgatatcga taccgccttc tttgtcaata aatactcgta agaaccagct ttttccttct
     8101 tttacaaatt cgacttcgac aagttcaaaa ttttgttcat ctaaaatcgg tgtcaccatt
     8161 tctgtgactg tttcaacgac gctactcaaa agtttcgcct ccttaaaaat taagtgcgta
     8221 attccattaa aaagagtgag cgaaaaatcc gctcactcac agttagtatc attacctata
     8281 agaactatac catactctgc ttaaaattac aagcagacag gaaaacagat gcttatagca
     8341 tatcgaataa cgataattga ttttcatctg gtagatcttt caatacgcca ttgtcgttca
     8401 tatattcgat caatgttttt gaaactttac cgcgggtggc aagatcttct ttggataaaa
     8461 atggaccgtc ttttcttgct tcaacgattt gtttcgccac gttggttccc aaacttggga
     8521 cagctctaaa tggtgcgatc aacgtatcac cttcaataac aaagttaacg gcatccgatt
     8581 tatacaaatc aatcattcca aatttgaatc ctcgttccag catttcattg gctaactcca
     8641 gcactgtcag ttgattcttt tcttttacgg aagcatctaa tcccttgtcc gtgatttctt
     8701 tcattgcttg tttcacagca tcttttcctt tgcacatgga gactagatca aagtcgtctg
     8761 cacgtacaga aaagtatgca cagtaataca aaatagggaa ataaaccttg aaataagcga
     8821 cacgaagagc catcaatacg taagctgcag catgggcttt cggaaacata tatttgattt
     8881 ttgaacaaga atcgatatac cagtcgggca cattattttc tttcatcgcc gatagatagg
     8941 tttctcgcaa ttcatcaggg atcttgttcc attgtccttt acgaacagtt tccatgattt
     9001 taaatgccat cccgctatct aatccagcat ggatcaaata aaccataata tcatcacgac
     9061 agccgattac ttccgccagt gtcgcatctc ctcgtcgaat caattcttcc gcatttccta
     9121 accatacgtc tgtaccatga gatagtccgg aaatttgcag tagttctgca aaagttgtag
     9181 gatgcgtttc ttctaacatc cctcgaacaa aacgagtgcc gaattctgga atacctaatg
     9241 tccctgtttt ggaataaatt tgttcctgac ttactccaag tacttcagga ccggcaaata
     9301 tccgcatcac ttccgggtca tccgtaggga tcgtttgtgg gtcgatccct gataaatctt
     9361 gaagcatccg aataacagtt gggtcatcat gccctagaat atctaatttc aatacattat
     9421 cgtgaatcga gtggaaatca aaatgagtcg ttttccattc tgaattttga tcgtctgctg
     9481 gatattggat cggcgtaaaa tcatagacat ccatataatc gggaataacg atgatccccc
     9541 ctggatgctg gccggttgtc cgtttgactc cagtagcgcc ttttgctaat ctgtcgactt
     9601 ctgcacttcg aaactgcaga ttattgtccc tttcgtatcc ttttacatag ccatatgctg
     9661 ttttgtcggc gactgtacca atcgttccgg cacggtaaac atagtcttcg ccaaacaata
     9721 ctttcgtata gttatgggct tctgcttgat aatcacctga gaagttcaaa tcaatatcgg
     9781 gcactttgtc tccatggaat cctaaaaatg tctcaaatgg aatatcatga ccgtctttgt
     9841 ttaagcgtgc accacatttt ggacattgtt tttctggcat gtcaaatcca gaaccatacg
     9901 taccatcttc aaaaaactcg gaatattggc actctggaca ataataatga ggggctaatg
     9961 gattaacttc agtgattcct gtcatcgtcg caacaaagct agagccaaca gagccacgtg
    10021 aacctactag atagccatct tcattacttt tatgcactag tttttgggcg atcagataaa
    10081 taaccgagaa gccattacca ttaatagaat tcaactcttt ttctaatcgt ttttgaatga
    10141 tctcaggtaa cggatctccg tacatttgtt ttgcttttgt gtaactcaat tctgatattt
    10201 catcttctga acctggaatc ttcggtgtat aaagttcatc tttgactgga atcacttctt
    10261 cgcagatatc tgcgatttta ttcgtatttt ctactacgat ctctttcgct gtttcttccc
    10321 ctaaaaagtg aaatgcagtt agcatctcat cggttgttcg gaaatggaca tccggcaaac
    10381 tatgacgatt caacggattc gctccgccca tagaattgat caaaattttt cggtaaatgg
    10441 catcttcttc gttcagataa tgtacattac ctgttgcaac aacgatttta tccaaacttt
    10501 ttccgatttc taccaaattt tggataattt cttccaaatc atgttcattt tttaccaatt
    10561 cttgctcgat caaaggcgca tagaccgctt ttggcataac ttcaatataa tcatagaatt
    10621 ttgctcggtt tcttgcctct tctacccctt tttgcatcat ggcttcgaat atttctccct
    10681 tatcacaagc tgagccgatc aatagatttt cacgcatctt tttcaattgg gagcgtggta
    10741 ttcgtgggac gcgttcgaag tattcgacat tcgacattga aatcagtttg aacaaatctt
    10801 tcagtcctgc ctgatttttt gctaaaatcg ttacatggaa aggtcgagcc cttttgtaag
    10861 aatctccttc tccgatatgc tcatttagct gatcatgata aagcatgtta tgattgtcca
    10921 ttgcctcttt cacaaagatc catgctaagt gaccagttgc ttcagcatca tagattgctc
    10981 ggtgatgctg ttccaagcta acaccaaact ttttagataa cacacctaat ccgaaacgtt
    11041 tgaattgagg atacagatat ctagctaatt ccaacgtatc aatgactgga ttggccgctt
    11101 caggaatacc ataccttgcg tagctagtat tcaagaaccc catatcaaaa gccgcgttgt
    11161 gggctaccaa aattgtatct tttgaaaatt caaggaacat ccgcaatact tcttcttccg
    11221 actttgaccc tcgaaccatt tcatccgtga taccagtcag atctaccgtt gttcgtgaca
    11281 atggatgtcc tggatcgata aattcatcaa agctttcgat cacatttcct ttatacattt
    11341 tgactgcggc tagctcgatg atcgtgtcat atactgcaga caatcctgtc gtttccacgt
    11401 caaatacgac gtatgtggct tcactcaatg cttcatgtgc atcattataa gcaattggga
    11461 caccatcatc tacgacattc gcttccacgc cgtataagat tttcacacct gctttttttc
    11521 cagcactatg tgcttccgga aaggcttgcg caccaccatg gtctgtaatg gcgatagctc
    11581 ggtgtcccca ttttcctgct tgtgccacaa gatcagagat gctgtttgtt gcatccatcg
    11641 tactcatatt gctatgaaca tgaagctcta ctcgtttttc cccttcagga gcataatctt
    11701 tgcgaggtgt atgtttgact tctatgatat cttgcgcatt catgaccaaa tcacggacaa
    11761 acgtatcttc ttgaatactt cctcgtacct ttaaccagct accgacactg atcgcatcaa
    11821 atacttgttc atctttttcg ccgttcgaga attttttgac gataaatgat gaagtataat
    11881 ctgtgatttt caaagtcaag atcttgcgtt ttgaacgtag ttctctgact tctttgtcga
    11941 atacgtaccc ctcaagtgtg actcttcgtt cttcttcaac gatactgatc attggtgtcg
    12001 ttggttcatc ggctggaata tttcgtccta gctggatggg tccttcaaga gctggcgatt
    12061 gctcttttct ttcctttttc ttttgttcgt ggacaactag actttcagct gcttgcttca
    12121 taaaggcttc tgcctgttct tgtttgcgtt cttcaaaaag tttcaataca cgttccgctt
    12181 gctgctcatc cacttctggt tcgatacgaa attttgggaa tccgtaactg acgaacaact
    12241 cttctactaa tggcaagtat tgttgcttca aataagaaat agctcctgta ctgtcgattg
    12301 gcaagatgac tttacgatct tttatcacag gtacctgcgt tttcaatact tgctgtgcta
    12361 gtggtgtatc gcattgctga ttttccaaag cttgggccca gtaatcttgc aagagctgtt
    12421 catcgaatgt ctggtcatct gctaagatat tcagtttgac ttgtgcaata tctttaaaag
    12481 cgatagtcag atgctgagtc aatgaccgat atagcataat aggcaaaatt ttggaaaaat
    12541 gtagcgtaaa ttcccataaa cgcgatttac gatggaccac tacactatcc atttcaccag
    12601 cagtaagcag tggatgattt ttttctgctt cctctagttg gatttgttcc aacagttttt
    12661 caaataaatc tcgtttttct gacaaagata tacgcccttt ctgatgagaa acaatgcaat
    12721 tgatcgcatc ttatcctctt ctttttcagt accttttagt atactcgttt tttctgagtt
    12781 ttacattaaa aaattggaac tgcgattcaa atcgcagttc caattttaaa agcgaatcat
    12841 cagattgtca aaaacagctg attcagctat ttttattttc tgtgttttaa cgcttattat
    12901 tcgccttggt tcaagaggat tggtaaagta ttcgccaatt cttctttacg tacttcaacc
    12961 atttcgccag ttttcttgat tttcacttct acgattccat ccactgcttt tttaccgaca
    13021 gtgattctga ttggacagcc aatcaaatca gaatccgcga atttcactcc tgcgcgttca
    13081 tttctgtcat caaccagtac ttcatagcca gcttttgtca tcgcttcttc tagttcattc
    13141 gtcaggcttg tttggtattc gtcttttaga ttcatttgga cgatatgcag atcaaatggc
    13201 gcaattcctt ttggccaatt gattccttgt tcatctgaat gttgttctac gattgctgat
    13261 aataaacgac ttacaccgat accataacag cccataatca caaatttttc tcgtccattt
    13321 tcgtctagta cagttgctcc catagattca ctgtaacgag tgccgatttt gaatatatgt
    13381 ccgatttcga ttcctcgagt aaaggcaaga acaccgttgt tgtctggtga aggatctcct
    13441 tcttgtacaa aacgaagatc ttcataagaa agcacagtga aatcgcgttt tggattcaca
    13501 tttttatagt ggaatcctgt ttcgttcgct ccagtaatgg cattcgctaa atcttggaca
    13561 taacggtctg catatacttt tacgtcatct gaaacaccga ctggtccgac agaaccaaac
    13621 tcagcaccta gatatttttg tgcttcttct tctgttgctt cttctaaaaa gtcagcagat
    13681 aaaaagtttt tcagttttac gtcattgact tcatggtctc cacgtaccaa aacaaggaca
    13741 ggttgttcat ccgcaatgaa caatacagac ttgatgattt tttgaggttc aacagaaaag
    13801 aaatctgcta cttcgtcaat tgattttacg ttaggtgtag ctactttctc caattcaagc
    13861 tgtgtttcat gcgatttttt tgatacatag tagcttgtag ccatctccaa gtttgcagca
    13921 taatcacttt cagtcgaata acagatcgta tcctccccga tttctgagat cgccatgaat
    13981 tcctttgagt ctttcccgcc cattgcgccg ccgtctccga tgatcgagcg aaattctaag
    14041 ccacaacgct tgaatacttc ggtataggct ttttcgtaat ctttatatgt ctcgtccaaa
    14101 ctttcttccg ttgcatggaa agaatatgca tctttcatga taaactctct tccgcgaagc
    14161 aaaccgaagc gaggtcgttt ttcgtcacga tatttaggtt ggatttgata aagatttaaa
    14221 ggtaatttct tatatgaatt gatttcatta cgcatcaaat cagtaaaagt ttcttcgtga
    14281 gttggtccaa ggatcatttt tcgatcattg cggtctttga aacgatacaa gtttggtcca
    14341 tatgtttcgt aacgcccgga ttcttcccaa agttctgctg gcaagatggc aggcatcagc
    14401 atttctaccg ctccgatttt ttcaaattct tcccgcataa tgcgtttcag tttttccaat
    14461 acacgatttg ctaatggcag atatgaataa atacccgctg atacttggcg gatatatcca
    14521 gctcttagca agatttgatg gcttaatact tctgcatcat ttggaacttc gcgtaatgtt
    14581 ggaatcaaca tttttgattg tctcattcta tatactcctt tatttcctca aaatagttat
    14641 cgtttgttat tataccctat ttgtcaactt tttctagaaa aagaatcgtt gaatatcgtt
    14701 ccatgtaact agcaccatca acaacattaa aaatccaaag ccagctaatg tcaatattcc
    14761 ttctttttct tgacttaatg gtttcccgcg aatcccttca aaaatattca agactaattt
    14821 cccgccatct aaggcaggaa tcggaagcag attgacgatt ccgagattca ttgaaagaag
    14881 cgccatcaaa ctaataacag ttgttacacc ttgatttgct gcttctgaag acaattggta
    14941 catcatgaca ggtccgccta atttatctaa actgaaacct gtaaatagtg atcctaaggc
    15001 tttgaaaatt tccaatgaac cgctgaaagc ttgacgcgtc cctccaatga tcttatccat
    15061 aaaacctgta ttcattgggg cttttatacc aagttggcca actttttcgc catttgactc
    15121 tacagatttc ggtgtcacgt cgacagattg cacctgacct tctcgttcga tcttaaagtc
    15181 taatgtttta cctggatttt tcgtaatgac tgttgtcaga tcattccatg aatggatttc
    15241 ttttccgtcc acactgacta cctcatcatt ttcttttaag cctgcttctg ccgcagctcc
    15301 attaggcatg atctctccta cgcgattagt gttcgtcact tgaacaccac cttgcataaa
    15361 tgccagaatg atgaataaca cgattgccaa aataaagtta ttcatgggtc cagcaaagtt
    15421 ggtcagcata cgctgccaca atttagccga ttggaactgt acgtcttttg gcgcaattct
    15481 gatttctgtt ccgtcgtttt caataatcgt cgcatcgtga tcaacagggt aggttactac
    15541 ttctgtttcg tctccattca cataaccagt gatagtcagc tcatcttcta aatcataacg
    15601 gctcaattcc atcgggatag catttgtcaa ttggattttc ttacttaaat ttatcttctc
    15661 tacgatgccg tcagaattta gtaaaagaga aagaggcatt cctggtgcca tttctgtttc
    15721 atcatcccca ttgcctgcca ttcgaacata accgccaata ggtaataggc gaagcgtata
    15781 tgttgtcccg tcttttgctt gatgcccata aattttcgga cccataccga tagcaaattc
    15841 gcggacgagg attcctgatc gtttcgcaaa gaagaaatga ccaaactcat gaacaatcac
    15901 taatatacca aaaacgatga taaatgtcag aatcgttttc atagcttttt tctcctttac
    15961 ttgttttttg tgttttttat aaaacaacga taaaacacta taattttacc atagtatacc
    16021 aaagacacca atagtatcaa tataatgaaa aataaaaacc tgcgtctttt atgaaattca
    16081 caaagaaacg caggtatact tactaaataa tgccaaataa atgcatgata ggaaatacaa
    16141 acagcaaact gtcgaaacgg tctaagattc caccatggcc tggtaagata tttcccgagt
    16201 ctttcacatc ataatgacgt ttgattgcag attcgaccaa atcaccaaat tgtccgactg
    16261 ctgaaaaaat aatcgtcatc agaagcatga caaacatacc atgattgaat agttcttttg
    16321 ctgggtatag caataaataa agaaatgcga ctacaacagc actaccgata ccaccgagcg
    16381 caccttcaat cgttttgttg ggcgaaatat caggacttaa cttatgctga ccataacgtc
    16441 gaccaaccat atatgctccg atatcagttg cccaaacaat aaatagacca aatatcaaaa
    16501 cagccaagct ttctgttcgt gcattgacaa aattctggaa acctattccc acatacaagc
    16561 tgacaagcac aggaaaacct gcttcatcaa tcgtatacgt atttttagaa ataactgatg
    16621 ttcctaaaag aatcataact ataagataaa ataacataaa tgtacttaca ttctctggaa
    16681 gaaagaaaaa ccatctttct tttggcaaaa caagtaacac tgcaccaacg gcggataaaa
    16741 ctccttcaaa acttaagagt gtcaatcctt tcatccggaa cagctcgtaa actcctacta
    16801 ccgctaagat tgctgctgta agctcaaccg ctatgcctcc gatccatata atcggaataa
    16861 acaagactag cgccacagct gctgtgatca cacgttgttt catgactgat cccctttctc
    16921 atcttctgtt ttttttacac cgccgaatcg acggtctcga ttttgataag aggcgattgc
    16981 ttcttcaaga tgcgcaccat caaaatcagg ccaaagcgct tttgtaaaat acagctcact
    17041 atatgcgatc tgccataaca gaaaattact aattcgctct tctccacttg tacggatcat
    17101 cagctcagga tcttgtaact ctttaggcaa gaaacctgtc attaaatgct tagcaatcaa
    17161 ttcatcatcg atttcttctg cgtgtatttc tccttttgct acttcttcgg caatttcttg
    17221 gacagcagtc acgatctctg ctctacttcc ataattcaaa gcaaaattca ataccattcc
    17281 agtattgttt tttgtttgct caattgcacg tcttacagca tcttgcgtat gttctggcaa
    17341 tacgttttca taacccatga catgaacttt gacattttct ttaatcaatt ctggtacgaa
    17401 cgtatcaaag aagtcgacag gtagttgcat gagaaaactt acttcatctt ttggtcgctt
    17461 ccagttttca gtcgaaaagg cataaagcgt caacactttc actccaagcc ttgatgcttt
    17521 ttttgtgact ttctttactg tttccatgcc ttctttatgg ccagcaactc gtggcaaacg
    17581 acggttttgc gcccaacgac cattgccgtc catgatgatg gcaatgtgct gaggaattgg
    17641 tccatcggta ttaaattgaa attcgctttt ttcttgaata tatttgtttt tttgcggaaa
    17701 aaaacgtaac attctttttc ctcctatact taaaagattc ccactatctt aagtattata
    17761 gcaatatcta actgtttttc ctatgaaatc attgtttttt tatttaaaaa ctgaataatt
    17821 ttttcttttt ttaattttta aataaaagaa atataaataa aaacagagga agcggaatga
    17881 tccgcccctc tgtttttatg attttattaa acttccaata attctttttc tttatcagca
    17941 gcgatcttat ctacttcttt aatgctgtta tctgtcaatg tttgtacgtc tttttctaat
    18001 ccgcgcaaat catcttcggt aatatcacca tttttttgtt gttttttata ttcatcgatc
    18061 gcatcacgac ggatgttacg aatcgcgatt tttgcatttt ctgcttcttt tttcacatct
    18121 ttagctaatt cttttctgcg ttcttctgtc agttgcggga taactaagcg aatgacattt
    18181 ccatcattgg ttggactgat tccgatatca cttgccataa tagctttttc aacatcttga
    18241 agaatgctct tgtcaaaagg tgtgatcatt aaaacacgtg cttctggaat ttggattgat
    18301 gccatttggt tcaatggtgt tggcgcaccg tagtagttca cagtaatacg gtccaaaagg
    18361 ctagcgttcg cacggcctgc gcggatttgc ccaagttcac gttgaagatt ttgtgctgct
    18421 tttttcattt tttcttttgc ttctgtcata attgcatctg ccatttttat ttccccctta
    18481 cagttgttcc gatattttcg cctaagattg cacggcgaat atttccgtgt tcatttaaat
    18541 tgaagacaag cagtggaata tcattatcca tgcttagtga acttgcagtt gaatccatga
    18601 cttgtaatcc tttggcaata acttccagat gagtcagttc ttcaaatttt actgcatttg
    18661 catctacttt cggatcagca gaataaacac cgtctacatt gtttttagcc atcaggataa
    18721 catctgcgcc gatttctgcc gctcgtaatg ctgctgttgt gtctgtcgag aagtacgggt
    18781 tgccggtacc gcctgcaaag ataacgatcc gtcctttttc taaatgacgt tctgctttac
    18841 ggcgaatata tggctctgcg atttgacgca tttcaatcga agtttgcacg cgtgtcggta
    18901 cacccacgtt ttccaacgta tcctgtaaag ctaaggcatt cataactgta gctaacatac
    18961 ccatgtaatc agcttgagca cgttccatgc ccatttgtgc tccgatttgt ccgcgccaga
    19021 tatttccgcc acccacaacg attgccattt caacgcccag ctcgtgaact tctttgattt
    19081 cttggatgat ttctttgatt actggtggtt tgattccaaa tcctgcatcc ccagctaaag
    19141 cttctccact caattttaag actacacgct gatatttcgg tgtgaccatg ttcattcctc
    19201 caatattttc tattgccatt ttaccatact tagatgattt cgacactcta agactggttt
    19261 ttcttgtttt gcttaaaaaa agggagcgca caaaccgtgc gttcccgtct gttattcaac
    19321 agaattattt tttcacttgg ctcataactt cttctacaaa gttatcttcg cgtttttcga
    19381 taccttcgcc aacttcgaaa cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt
    19441 atttttcaac agtcatatca ggatctttaa cgaatggttg gtccaccaaa gcgatttctg
    19501 ctttgaattt cttcaagcgt ccttctacca ttttttcaac gatattcgct ggtttgcctt
    19561 cgttcaatgc ttgttctgta agaacagttt tttcatgttc taattcagct tctggaattt
    19621 gtgattcgtt tacataacga ggattgatag ctgctacgtg catagctacg tctcttgcaa
    19681 ctgtttcgtc agttgttcca tctagaacag tcaatacggc aatacgtccg cccatatgca
    19741 agtatccacc aaatgcagca ttgtcgtctt tttctacaac ttcaaagcga cggaagctga
    19801 ttttttctcc gataacttga gttgcttcga tcaagtcata ttcaattgta cctttatctg
    19861 ttttgatttt cattgcagct tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa
    19921 tttcttttac taaatcttgg aacatttcgt ttttagaaac aaagtctgtt tctgagttta
    19981 cttcaacgat tgctgcagtg ttacctttca cagcaactga agccaatcct tcagctgcaa
    20041 tgcggtcatt tttcttcgca gcctttgcca ttcctttttc acgtaaaaga tcaactgctt
    20101 tttccatgtc gccttctact tcaactaatg cttttttcgc atccatcatt cctacgccag
    20161 tcatttcgcg tagttctttt accattttag ctgtaacgtc tgccatgttt aactcctcct
    20221 gtgtgttttt ctttttaaaa aaagctgctt caaaggagag tgaggcttgt cgcccagcct
    20281 ttgaaacagc tccatcattc aaaattattc tgctgaagaa ttgtcgcctt caacaacatc
    20341 tacgatttct tcgattgatg ttgctgtgtt ttcttctgtg aaatcttctt caactacttg
    20401 atcttcacct tggtttcctt cgatgaaagc atcagccatt tttgaagtaa tcaatttaac
    20461 ggcacgaatt gcatcgtcgt ttgaagggat tactacgtcg atttcatctg gatcgcagtt
    20521 tgtatcaacc atagctacga ttgggatatt taatttgtgt gcttcttgaa cagcaatacg
    20581 ttctttacgt gggtcaacga tgtacattac atctgggatt cttggcatat cagcgatacc
    20641 gcctaagaat ttttcaagac gttcacgttc tttgttcaaa ccagcaactt cttttttagg
    20701 tagaacttca aaagttccat cttcttccat tgcattgatt tgtttcaaac ggctgatacg
    20761 tttttggata gtatcccagt ttgtcaatgt tccgcctaac caacgatggt taacgaagta
    20821 ttgtcctgca cgagttgctt cttctttgat tgcttcttgt gcttgttttt ttgtacctac
    20881 gaataatgct acgccgcctt cttcagcaac gctcttcatg taatcataag ctgcatctac
    20941 taatttaact gttttttgca agtcaatgat gtagattccg tttctttctg tgaagatata
    21001 tttcttcatt tttggattcc agcgacgagt ttggtgacca aagtgtacgc cggcttctag
    21061 taattgtttc atagaaatta ctgccatgtt tgtttcctcc gatttggttt tatttttcct
    21121 cttcttatct tctaccttgc cggcaaactt tttcaagcac ctaccgacaa tccaataaga
    21181 atgtggattt gctgatgcat aaagcaccct cagctattat acagacaaaa gatccgtatc
    21241 gcaagagcaa atatactttt ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa
    21301 ttcaagaaaa acagtagatt tctctttgtt tctttattat cctttttcca taaaataatt
    21361 ttaggtataa aagacgaata ttattgggca atccttcttt tttttgttat gatacatgtg
    21421 aaatctttcg taaaggagaa agaaaatgcg caaagctcaa agacatttgc tgattaaaca
    21481 agtcattgaa gaattcccga tccgtacgca agaagaacta ttaaacaaac ttgggcatta
    21541 tggcgttacc gccacgcagg caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa
    21601 ggcacctgat gaaaatggcg tttcccgctt cgtattgttt cgcgaaaaag gaactcctga
    21661 gactaggact gaggaagagg atcggctggt tcaaatgatt gaagacattg tattgaaagt
    21721 cgaacgagtc cactttttga cgatcgtcaa taccttgcca gacaatgctc atttatttgc
    21781 atctgtttta gatgagatca aacctccctc tattgtcagt acaattgctg gattcgatac
    21841 gatcatggtc atctcggaaa atgaggagga cgcaaaacga gtagaagaat ttcttcacca
    21901 tccaactgaa gtatcacttt tttaatcaga gcacgtattc ctaatatgga atgcgtgttt
    21961 ttttattttc aatattttcc ttattaaata aacctttaca ctctttacaa aaatacatca
    22021 ttttttattc aattttttca acaccttgat tgaatcacaa taatcatcat ttatattgga
    22081 tgttaacaag agcagcttct tgctctttgt gtatttcaaa aaagaaatga aaggaaatca
    22141 tatgtataaa ttattaactc ctggaccatt gacgacttct gaaacagtga aaaatgctat
    22201 gctgatcgat cattgtactt gggatcaaga atacaaagaa attacacaat ggataagaaa
    22261 aaaattattg gatcttgctc aagtatcgga aggaatatac acaacggtct taatgcaagg
    22321 tagcggctcc tttggggtag aatcggtatt aagttctgtg atcaggccag aagatactgt
    22381 actgatttgt tcaaacggcg catatggaca acgaatgatc aacatgtgcg aatgtctgaa
    22441 actgaactat accgtttatg aggtatcgta acatcaaatc ctccaagctc atgtgattac
    22501 acagctgatt gaagagaatc cctctatctc cgcactagca atggtccata gcgagactac
    22561 ttccggtatt ttgaataatt tggaagagat tgctgctgtc acacaagaaa aagatctttt
    22621 gtttatagtg gacgcgatgt caagctttgg tgggataccg attcgtgtag ccgaactagg
    22681 gattgatttt ttgatttcca gctcaaacaa atgtattcaa ggtgttccag gcttttcttt
    22741 tgtacttgct aataaagcgg aactagaaaa gcgaaaaaat gtgagtcgaa gtttgtcact
    22801 tgatctatat gaccagtacc gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc
    22861 ccctacccat acggttttgg cttttcaaca agcgctgaag gaactagagg cagaaggagg
    22921 aatcgaaact cgatattcac gttatctcgc caataatcat gtactacgac aaaaaatgac
    22981 agagatgggg ttccagcctt tcattcaaga atatcagggg ccattcatca cctcttttct
    23041 ttaccctaag gactggcgat ttgattttaa tcatttttat cagtatttaa agcaaaacgg
    23101 atttgtgatc tatcccggga aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg
    23161 cgagatctat ccggaagata ttttggaact gacagcaatt atcaaagact ataaggagaa
    23221 cgttaatgat taaaactgta atttttgatt gggcaggaac aactgtggac tttggctgta
    23281 tggctcctgt ccacgctttt agaaatgctt ttctagaaaa aggaatccaa ctgactgaca
    23341 aagaaatccg cgaaccgatg ggaaaattaa aatgggatca tatccaacag cttctttccc
    23401 tcccttctgt caaaaaacaa tggatagcta tttatggaca actacctcag gaaaaagagg
    23461 tggaggaatt gtatcaacaa tttgaacatt atctgtttaa agatttggtc aagcattcta
    23521 ccttgaaacc cgacacattg gaaacgatcg aacggttgag aaacgctggg atcaatattg
    23581 gttccacgac tggatttact gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag
    23641 aagggtattc accagacttc atggcaactt ctgaagacgt cgaagggtat ggtcgaccgt
    23701 ttccttatat gatccataaa aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa
    23761 tcaaagtcgg agatacggtc tcagatatcc aggaagggaa aaatgccgga gtgttgacag
    23821 taggtgtaat tgaaggtagt tcgctaatgg ggatttctta tgatgagtac caaaaattag
    23881 agatggggaa aaaagatcgt ttaaaacagt ctgttgctct tagattccaa caagctggcg
    23941 ctgatatttg tgtaaacaat ttaaaagagt taacagaata cattttgtac gctgacaaga
    24001 atccagaatg acttttatac aaaatcagag gtcgtgaaaa agctgtactg catcaagtaa
    24061 taagaacagc caaacaaaaa tagctcctca tatttttcgt ttggttaggc ttattaccgc
    24121 agatgcaagc ttttgaacac cgtttattcg aaaaaaaaag ggagctgtga cacgactttt
    24181 gtcacagtcc ccttatttac aaacaaacag tgtttaagag acaacctctc cactaaacgg
    24241 tgttcattca gctgactctc ggtatttgct caaaaaacga taaaatttga aaagctattt
    24301 tctcttttgt ttctctaata ctcggatcag aacgctgatt tcacattctc tttttcttac
    24361 atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt ctttcaataa tacagtcact
    24421 gcataaacta gagctaagcg tgcttctttt tgttcatcat cagctaaaat cttcgtatga
    24481 gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat gtttcgcaat cactgacggt
    24541 tcgtattttt ccccagcaga caagaccgtt tcagggtatt tttgaactaa tttaaccact
    24601 tcccagctgg tatcgtcatt caatgcatag tctgctgttt ctgaagggac aaatcctgct
    24661 ttttccaaca ggctgactgc acgagcatgt gtatattgca cataaggtcc agtttctcct
    24721 tcaaatcgaa caacttcttc caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa
    24781 tcgtggaaaa tgactgcccc aaccccaact tgtttagcta cagcatcttt gttttccaaa
    24841 tcagggtttt tctcgctgat ttgttcttta gcagattgga ttgcttcatt taatacttct
    24901 tctaataaga cgatctttcc tttacgagtt gataattttt taccaccttg cgtgatcaaa
    24961 ccaaatggaa tatggcgcat atcgtcagac cagtcaaagc ccatttcctt caagacagct
    25021 ttcaactgtt taaaatgata gctttgttca ttccctacta catatagaga ttgtttgaag
    25081 tcgtaagtac gtttacggta caatgcagca gctaagtcac gtgtaatgta tagtgtcgca
    25141 ccgtctgact tcttaatcaa cgctggattc aggtcataag cagataggtc aacgatctct
    25201 gcacctttat cctcgttcag caaatgtttt tcttctaaaa gtttgacgat ttcatccatc
    25261 ttgtcattgt agaaagcttc accgttcaac gaatcgaaac gtacttccag cagatcatag
    25321 attttattga attctttcat cgattcatca cggaaccatt gccataattg aatagcctct
    25381 tcatcgcctt cttccagacg tttgaaccaa gcacgagctt cttcatttaa ttctggttct
    25441 gtttcagcta cttcatggaa ttggacatat aaacgaagca attcattgat tggttctgct
    25501 ttaacagctt cttctgtacc ccattttttg taagcgacga tcagttttcc aaactgtgtt
    25561 ccccagtcac ctaaatggtt gatgcggatt ggctgataac cgattttttc cataatgaaa
    25621 ccgatcgaat taccgataac tgtcgaacgc aaatgtccca ttgagattgg tttggcaata
    25681 ttaggagaag acatgtcgat tggtactgtt ccttgattcc cgatattgct atccccataa
    25741 tgttcttttt ctttcactac tgtttgcaaa acttttttgc tgataagttc tttattcatg
    25801 aaaaagttca agtaaggtcc gacaacttcg attttttcaa agttggcaga atcgattttt
    25861 tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt aaacttttgc taatgaaaag
    25921 gctggaaatg caacatctcc gtgttcagct gattttggat tttctagtaa ttgttcgact
    25981 tgttccagtg ttaaatcatc tttgacagcg tcatagacag cttttgcgac aagatcttta
    26041 ttattcatct ttattcctcc taaagtgctc ccttgattgt taaacaaaaa aaatctctag
    26101 cgacggcata cttagtatgc catcactaga gacgagattt cccgcggtac cactccaatt
    26161 gtcctaaaaa aggaccagct ccatctgata tcggtagacg accgtctgtt taaaacagaa
    26221 ttctccaagt gcgcttcgtt gtatcttctt cattcggctt ccaccacttc cgaattcgct
    26281 agccaagaat gatacaagta ctctcttgtt caacaaatct tagtcttatt aaactttctt
    26341 gaattatatc agaaaaaaaa acgaaaggct agtcgtttct gataaaaaaa caatccagat
    26401 ggcctaaagg agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga
    26461 cttaacaaat taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat
    26521 attttctaaa gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc
    26581 tgcctctact ttcggaagca tgcttcctgg agcaaattgc ttttcatcaa tgtattgttt
    26641 catttctgaa acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata
    26701 cacattgtct actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt
    26761 ttctgaagca aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc
    26821 tacaacagga atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt
    26881 ttcgatgaca cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca
    26941 tccacgtcca gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc
    27001 ttcttcttcc gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt
    27061 ttcatccaca ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt
    27121 cagtacttcg cccatggcat tttgtaacca gtaaccgata gatccttctg tcattgcaac
    27181 acatgtatct aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa
    27241 taggttaccg acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat
    27301 aaatttcact aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc
    27361 atccgtagac aagattgcat taccccctaa agcgacaact acttttcgtt tggccatttc
    27421 attcgtcctt tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt
    27481 gattttccct caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg
    27541 agggataaac aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt
    27601 ttctgcttgg tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat
    27661 ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa
    27721 agctggcaag cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc
    27781 catattgatt tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc
    27841 cattgatacc catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc
    27901 gtcggtaatc atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat
    27961 ttcttcttca ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc
    28021 acctgtgact aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt
    28081 gataccttct actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt
    28141 tggatgccat tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc
    28201 ctctaccatt ttttgactga atccacggaa ttcgattcca tcaaacatac gacctagtac
    28261 tttagctgta tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata
    28321 ttcaggatgt gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga
    28381 tgttttttca aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg
    28441 ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt
    28501 aaagtctttt tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat
    28561 tctcctttag attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta
    28621 agaccgccgt caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca
    28681 gcgtggacca ccacggcctc gtgacaattc acttgaattg atttctaaca ctttgatgcc
    28741 atagctacga agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg
    28801 agcgattgcc aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc
    28861 tccattacca gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc
    28921 gtgtaagtta tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac
    28981 atccatctta ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac
    29041 cattgtaaat acggtatcca aatgcatcat tgcacggttg tttggaattt tgattgctaa
    29101 cactttttca aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt
    29161 cgcattagtt cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc
    29221 tccgccttcg atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc
    29281 aaatcttgga tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc
    29341 aaaagtcatc ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa
    29401 gtacaagttt ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcgta
    29461 tagactgcta gaacgtactt caatatcttt tgtacgaacc ccagccatga ttttatctac
    29521 catttcttgc gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga
    29581 tgcaatatga gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc
    29641 aatcgcttct gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt
    29701 ctttgcaaag ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa
    29761 cagacgatcc ataatatctg gtgtcaaatt ttccacttct tgtccaggcc ttttcagtaa
    29821 aactgtcttt aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg
    29881 cctccttctt tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt
    29941 tgtctc
//
LOCUS       DAYYCW010000022        29713 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000022 DAYYCW010000000
VERSION     DAYYCW010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.022"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1016
                     /locus_tag="ACHAYA_000946"
     CDS             147..1016
                     /locus_tag="ACHAYA_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8604766.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ACHAYA_000947"
     CDS             1225..2250
                     /locus_tag="ACHAYA_000947"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8604767.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ACHAYA_000948"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ACHAYA_000948"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM8604768.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3333..5036
                     /locus_tag="ACHAYA_000949"
     CDS             3333..5036
                     /locus_tag="ACHAYA_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8604769.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ACHAYA_000950"
     CDS             5038..7071
                     /locus_tag="ACHAYA_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM8604770.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ACHAYA_000951"
     CDS             7082..7522
                     /locus_tag="ACHAYA_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8604771.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ACHAYA_000952"
     CDS             complement(7722..8849)
                     /locus_tag="ACHAYA_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8604772.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ACHAYA_000953"
     CDS             complement(8852..9529)
                     /locus_tag="ACHAYA_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8604773.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ACHAYA_000954"
     CDS             9793..11559
                     /locus_tag="ACHAYA_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM8604774.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ACHAYA_000955"
     CDS             11574..12011
                     /locus_tag="ACHAYA_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8604775.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ACHAYA_000956"
     CDS             12024..13175
                     /locus_tag="ACHAYA_000956"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM8604776.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13237..13935)
                     /locus_tag="ACHAYA_000957"
     CDS             complement(13237..13935)
                     /locus_tag="ACHAYA_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM8604777.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13928..15280)
                     /locus_tag="ACHAYA_000958"
     CDS             complement(13928..15280)
                     /locus_tag="ACHAYA_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM8604778.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15503..16903)
                     /locus_tag="ACHAYA_000959"
     CDS             complement(15503..16903)
                     /locus_tag="ACHAYA_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8604779.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17064..17801
                     /locus_tag="ACHAYA_000960"
     CDS             17064..17801
                     /locus_tag="ACHAYA_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM8604780.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17926..18405
                     /locus_tag="ACHAYA_000961"
     CDS             17926..18405
                     /locus_tag="ACHAYA_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM8604781.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18402..19169
                     /locus_tag="ACHAYA_000962"
     CDS             18402..19169
                     /locus_tag="ACHAYA_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM8604782.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19399..19968
                     /gene="lepB"
                     /locus_tag="ACHAYA_000963"
     CDS             19399..19968
                     /gene="lepB"
                     /locus_tag="ACHAYA_000963"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8604783.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20072..20674)
                     /locus_tag="ACHAYA_000964"
     CDS             complement(20072..20674)
                     /locus_tag="ACHAYA_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8604784.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20780..21010
                     /locus_tag="ACHAYA_000965"
     CDS             20780..21010
                     /locus_tag="ACHAYA_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604785.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            21001..21249
                     /locus_tag="ACHAYA_000966"
     CDS             21001..21249
                     /locus_tag="ACHAYA_000966"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8604786.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            21355..21939
                     /locus_tag="ACHAYA_000967"
     CDS             21355..21939
                     /locus_tag="ACHAYA_000967"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8604787.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21942..23015
                     /gene="prfA"
                     /locus_tag="ACHAYA_000968"
     CDS             21942..23015
                     /gene="prfA"
                     /locus_tag="ACHAYA_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM8604788.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23008..23847
                     /gene="prmC"
                     /locus_tag="ACHAYA_000969"
     CDS             23008..23847
                     /gene="prmC"
                     /locus_tag="ACHAYA_000969"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM8604789.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23840..24019
                     /locus_tag="ACHAYA_000970"
     CDS             23840..24019
                     /locus_tag="ACHAYA_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604790.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24041..25066
                     /locus_tag="ACHAYA_000971"
     CDS             24041..25066
                     /locus_tag="ACHAYA_000971"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM8604791.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25257..26501
                     /gene="glyA"
                     /locus_tag="ACHAYA_000972"
     CDS             25257..26501
                     /gene="glyA"
                     /locus_tag="ACHAYA_000972"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM8604792.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26654..27601
                     /locus_tag="ACHAYA_000973"
     CDS             26654..27601
                     /locus_tag="ACHAYA_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM8604793.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27735..28364
                     /gene="upp"
                     /locus_tag="ACHAYA_000974"
     CDS             27735..28364
                     /gene="upp"
                     /locus_tag="ACHAYA_000974"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM8604794.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28503..28745)
                     /locus_tag="ACHAYA_000975"
     CDS             complement(28503..28745)
                     /locus_tag="ACHAYA_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604795.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28866..28938
                     /locus_tag="ACHAYA_000976"
     tRNA            28866..28938
                     /locus_tag="ACHAYA_000976"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28899..28901,aa:Thr,seq:cgt)
     gene            complement(29057..29695)
                     /locus_tag="ACHAYA_000977"
     CDS             complement(29057..29695)
                     /locus_tag="ACHAYA_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM8604796.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT        10115 a         5242 c         6048 g         8308 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gtctgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt ctggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccatgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaacgtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg acgaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcggaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggcag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatcca gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaagag
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttcgct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata
    13261 gggcagatcc aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc
    13321 caggttctca tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta
    13381 aatgacacct tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa
    13441 aggtcgttca ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt
    13501 ttcattgaat tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact
    13561 taacgtgtag tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta
    13621 gtagtgacct agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat
    13681 atagttgatc aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa
    13741 gtcttgacca ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat
    13801 actaacgatc tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa
    13861 aaggtttccg ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag
    13921 ttcataatta gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac
    13981 gaagctgcaa tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt
    14041 tgattttttc gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc
    14101 cggcaacttt tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg
    14161 ggatatccat atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc
    14221 cgtctgcgta attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa
    14281 tcatatcgat tacttgattg atacctacag gatttttgac aagtactaat gtacattttt
    14341 tcccattgat attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt
    14401 gtttgatttt atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat
    14461 tgtagacatt atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat
    14521 ctgctgacac attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt
    14581 tgaagtcaca atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat
    14641 gcaagatgtg atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt
    14701 cacgatcttc ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat
    14761 tgaagattgg tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt
    14821 ctacaatcat tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa
    14881 tatttgtaaa taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt
    14941 catctatttc taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag
    15001 tcgatacaat tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt
    15061 gctggcgcaa aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga
    15121 caacgacgac ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg
    15181 ctagtttacc tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg
    15241 tttttccaac tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga
    15301 tttgctgacg aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt
    15361 ttcttattct taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga
    15421 tttaacaaaa aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt
    15481 ccctcttacc tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc
    15541 ctagatctcg tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc
    15601 caataaacat cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc
    15661 ctaaagataa accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg
    15721 tttgttcttc tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca
    15781 taaagacaac gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga
    15841 tgttatcttc acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg
    15901 ttcgaatccc gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa
    15961 tgatcagtgt tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag
    16021 tcgtaatcga ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata
    16081 aatggtcctt ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag
    16141 ctaacacagc taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt
    16201 tgtataatag ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa
    16261 tcaaaaacat gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc
    16321 cagtgacgtc aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa
    16381 cggcatcacg ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc
    16441 caaaaagtaa ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac
    16501 cagacaaacg cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc
    16561 gtcgtttaga aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt
    16621 gaaataatat ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag
    16681 tcgatagtcc agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa
    16741 catggctatc tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca
    16801 tcaatatata ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct
    16861 gtcctcgccg ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac
    16921 aatagtcgtt tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca
    16981 gataacaata cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa
    17041 aaagaaggag gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa
    17101 atcaaaaaat aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag
    17161 aagtaaagcc taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg
    17221 aattcatgtc tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta
    17281 aaagtgagta tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga
    17341 aaactgacca tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa
    17401 tggttttaac aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct
    17461 tcaatatgcg attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg
    17521 gaagtgccct gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg
    17581 atatccaaaa gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct
    17641 atgaacgctc tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt
    17701 gcgtgttcgc tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag
    17761 cttacttaaa aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac
    17821 gtaagaaaaa aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag
    17881 taaagtaagg aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca
    17941 cctattttga agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt
    18001 attgtgaata gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca
    18061 acaccaacta cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc
    18121 actacgacaa gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca
    18181 actgacagct caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc
    18241 aatccaagta caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact
    18301 gtagatgata acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca
    18361 ggaaataccc aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc
    18421 ttatcaacgg ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc
    18481 gtggatatac gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac
    18541 tttggcaaaa ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg
    18601 tttattttac tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg
    18661 tcactcagct ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat
    18721 cgatagataa tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg
    18781 acgaagatta tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga
    18841 tcaaaacaaa tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc
    18901 cttattacta tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct
    18961 actcggacat ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac
    19021 ctgtcggatt gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc
    19081 cttatcgtct gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata
    19141 gtcgtagcca aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg
    19201 ataaaaacgt ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt
    19261 atataacgag aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt
    19321 gtagaataaa gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat
    19381 acaagtagga ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta
    19441 ttagcggcta ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat
    19501 tcgatggacc ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat
    19561 gaacgaatgg atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa
    19621 cggatcatcg gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat
    19681 ggcaaaaagt atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag
    19741 ttacaaggag aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc
    19801 tttacgagtg actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac
    19861 cgcttgattt caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga
    19921 aaagttgttt tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa
    19981 aagaagctgt gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct
    20041 tggagttgct cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa
    20101 gcgaagcgtt tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag
    20161 agacaaagcc cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga
    20221 ctttcggatg actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag
    20281 gagctaaaat ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta
    20341 caagtgatag aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt
    20401 ggatcgttcg gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca
    20461 tagtttctga agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa
    20521 caagcccagt tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat
    20581 aagtagttag ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat
    20641 ggttagcgat ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg
    20701 aaattttaac ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa
    20761 gggagaatct agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa
    20821 gtgtgtccga gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga
    20881 aaaatatatc gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata
    20941 tttaggcttt atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt
    21001 atgggaataa aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca
    21061 gaaggttacg cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac
    21121 tactctatat atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa
    21181 gtttttccaa atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa
    21241 caaaagtgag caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa
    21301 atgctgaaaa tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca
    21361 caactatttt ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc
    21421 gcacataatt atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca
    21481 agagatggtt taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata
    21541 tttgaagaaa cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg
    21601 ctgatcgatg aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg
    21661 gacgaattgg atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta
    21721 tttgaaggat caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc
    21781 tgttggttct gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt
    21841 tatgaaggcg atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa
    21901 cattactttc acccatcaat gattacagaa ggagaataag catgtacgat caattacaag
    21961 caattgaaga ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg
    22021 atacaaagcg ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag
    22081 tttatcgtcg ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg
    22141 aaaaactgga tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag
    22201 aaaaagaagt cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg
    22261 ataaaaatat catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg
    22321 ctggcgatct tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag
    22381 tgatggaagc taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg
    22441 gagacaacgt atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc
    22501 cttcaactga atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag
    22561 aagctgaaga agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg
    22621 cttcaggtgc tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact
    22681 taccaacagg gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa
    22741 aagcaatgaa aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg
    22801 agtatgatgc caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga
    22861 cgtataattt cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat
    22921 tagatcagat tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc
    22981 aaacatcaaa actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca
    23041 cgggcttctt cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt
    23101 ttggaacgaa aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca
    23161 gaagaagaac agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct
    23221 cagtatttgc taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca
    23281 ctgatccctc gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt
    23341 gtaccgttag aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa
    23401 gcagcaagaa aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta
    23461 gcgaaagaaa atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta
    23521 ggaccagtaa tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg
    23581 aatgaatggg aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt
    23641 gctgagaacg atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta
    23701 aaatcaaatg gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa
    23761 atatttcagc aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat
    23821 gaacgaatga tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt
    23881 tattataaaa aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt
    23941 ttatccacta ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag
    24001 acaaaataag aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga
    24061 tgaaacagaa ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc
    24121 taccgaaaca gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt
    24181 gttttctgta aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga
    24241 acaagtaaaa gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt
    24301 ctggcctgga ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt
    24361 gacgggagga ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt
    24421 aatcgaactt tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag
    24481 tccaacgact gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga
    24541 tgatggagct acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc
    24601 catgcctatg attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga
    24661 atctcctgtt gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg
    24721 gatgaaatac aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg
    24781 gtcaactgca gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc
    24841 cgaaattgcg gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc
    24901 agtagaagcg gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact
    24961 tggattagat ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat
    25021 gaatcgcttg aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg
    25081 tcgcattctt ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa
    25141 ctaagcgtaa ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg
    25201 aaaaataaaa ttgtaggaaa ataaaattgt aggaaaataa aaaggagctg ttgtttgtgg
    25261 tagattacaa aacgtttgac cctgatcttt gggctgcgat cgccaaagaa gaagaaagac
    25321 aagaacataa tttggagctg attgcctctg aaaactttgt ctccgaagca gtgatggctg
    25381 cacaaggaag tatcttgaca aataaatacg cagaaggtta tccagggcat cgctattatg
    25441 gtgggtgtga atttgtcgat atcgttgaaa atttagcgat tgaccgtgca aaagaattat
    25501 tcggagcgaa atttgcgaac gtccagcctc actccggctc tcaagccaac acggctgctt
    25561 atctcgcttt ggtagaacca ggggacacga tcttaggaat ggatctttcc gcaggcggac
    25621 atctaacaca tggatcacca gtcaacttca gtggcaaaac ctatcacttt gttgcttacg
    25681 gtgttgatcc gacaacagaa gttattgatt ataatgtggt acgtatttta gctagaaaac
    25741 atcaaccaaa attgatcgta gcaggtgcca gtgcttacgg acgaacgatc gactttgcga
    25801 aattcagaga aatcgcagat gaagtaggcg caaaactgat ggtagatatg gcacatatcg
    25861 caggattagt agcagcagga cttcatccta atccagttcc ttatgcagat atcacaacga
    25921 ctaccactca taaaacattg cgtggccctc gaggcggcat gattttgaca aacgatgaag
    25981 cattagcaaa aaaaataaat agtgcggtat ttcctggtat tcaaggcggc cctctagagc
    26041 atgtgattgc gggtaaagca gtagcattca aagaagcatt agatcctgca tttaaagaat
    26101 atagtgaaca aatcatcgcc aatgcaaaag caatggtcaa agttttcaat caagcaattg
    26161 gaaccagagt catttcaggg gcgacagata atcatctgat gttgatcgat gttcgcgagt
    26221 taggtataaa tggaaaagaa gcagagagca ttttggacag tgtgaatatc actgtaaata
    26281 aaaactcgat tccttttgaa acactaagtc cattcaaaac aagcggtatt cgaatcggca
    26341 cgccagcaat cactactcgc gggttcaaag aagaagatgc tgtaaaagtg gctgaactag
    26401 ttgtcaaagc attacaagca aaagatgata acgctcaact agacgaagtc aaaacaggtg
    26461 tacgcgaatt aactgaaaaa tttccgttgc ataaaaaata aaaatttaaa ttccattagt
    26521 aaacagaaaa gttcaatgtt gggcttttct gtttattttt tgggaaaacg gattctttga
    26581 caatatcggt gatgctctct agtatatgag gtgtaatttt agtcttcaca ctaaatctca
    26641 gggaggaaaa gtcatgactg atcgtatctc gttagaaaag gaagcagtag cattcagtga
    26701 tgcaaatgcg cctcatccgc gtatctatga actagcaccg gaagaaggaa gagttttact
    26761 agaagaagta caagaatcac ctgtgaacaa atattccgtc gatattgaag acttgatggt
    26821 ggatactggc gaatggggaa aactcaatat tcgtctcgta cgaccagaag ggaatatgga
    26881 taaattacca gttatttttt atattcatgg agcaggctgg gtttttggca gtgctcaaac
    26941 ccatgataaa ttgatcagag aacttgcggt tcgtacgaat tccgtcgttg tctttccaga
    27001 atatagtcga tcaccagaag caaaatatcc aacagcaatt gaacaaaatt atgctgtgtt
    27061 gcaacagctg aataaattag ccaagtcaaa aaatctggat ctcgagaatc taatcgttgc
    27121 aggagattca gtaggtggta acatggctac agtcatgacg atcttaacaa aagaacgaaa
    27181 gggcctccca atatccaagc aattgctgta ttatccagtc acaaacgctg attttgatac
    27241 agattcctat catgaattcg gagaaaacta ttttttgaca agagaaggaa tgatgtggtt
    27301 ttgggaccaa tatacgacga atgaagaaga gcgtgctgaa atcacagctt cgcctttgag
    27361 agcaaatata aatgaactaa atgatcttcc ttcagcaatg attttaacgg gagaagcgga
    27421 tgtattaaga gatgaaggtg aggcttatgc gcgcaaactt cgtgatgcag gggttcctgt
    27481 tacacaagta cgcttccaag gaatcatcca tgattttgtc atggtaaatg ctatggatca
    27541 gacaaaggca acgcgtgcag caatggcatt atctacttct tggatcaaga aaaaagaata
    27601 agctttgctt gtccgcagga aaacttcttt tcaaaaacgg attttcttag gaaaaatggc
    27661 gagtttgact agtcaatcac ttgattttgt tttacaatta aaaagaaacg acagaacaaa
    27721 ggagagctta aaaaatgggc aaatttcaag tgatcgatca tccattgatt caacacaaac
    27781 taactattat tcgggacaaa aactgcggaa caaaggtttt tcgtgaagta gtcgatgaaa
    27841 tcgctatgtt gatggcatat gaagtatcac gggatatgcc attggaagac atcgtgatcg
    27901 aaacaccgat cactgaaaca acacaaaaaa cgttgtctgg taaaaaagta gcgattattc
    27961 caatcttgcg cgcaggtatt ggaatggttg acggtatctt ggaattgatt ccagctgcta
    28021 aagtgggaca tatcggtctt tatcgagatg aagaaacatt ggaaccacat gaatatttcg
    28081 tcaaacttcc agaagacatt gatgcacgtc agttgtttgt agtagatcca atgttggcaa
    28141 caggcggatc agcaatcatg gcaatcgatg cattgaaaga acgtggcgcg tcgaacatta
    28201 aatttgtttg tttggtagca gcacctgagg gagtaaaagc tttgcaagaa gcgcatcctg
    28261 atattgacat ctatacagct tctctagatg aaaaattgaa tgaacatggt tatatcgttc
    28321 ctggactagg ggatgcgggt gaccgtttgt ttggtacaaa ataagtaaat acgaaaaagg
    28381 gtgtgacaca acttttgtca catccttttt tggtcatttt taacgtttga tgcagaacag
    28441 ctttttcaca gctttcgttt gcatataaaa aaaccatccc aaaaatgcag agatggtcaa
    28501 attcaaatct tttttagttc tttgtagtta acgattacta cacccttact ttgcgtcatc
    28561 aagttcgcat tttctgcagt ttcatcaatc tcaacaacag cggaattttt caattgcttt
    28621 gaaatcgttc cagtttgcga cttgccgtga attgtgaaca ttacgtgagt attgatcttg
    28681 aatttttcac caatttccga tggtacgata gttgtatcaa attttccaaa agttgccatt
    28741 ttcataaaac ctgcctttca catcaattat accacgtttt atgaaaaata tcatgaaatt
    28801 aaactaattt tccatcggac tctttacaat cagccaaata gagtatataa tttcttttgg
    28861 ataaggccag cttagctcag ttggtagagc aacgcactcg taacgcgtag gtcgtaggtt
    28921 cgatccctat agctggcatt aaaagagaag acagatatat caaggatttt taatccattg
    28981 atacatctgt ctttttgttt aaaggaaata aagtatgctt tcttagcaat agagcatgta
    29041 ttatttctat aaaaatttag gagaataaaa cgactagcaa tatagctgtg aaatcaaata
    29101 tcacatgaac tgcccaactt gtaatgatcg agttgctttt aatggcagct tggttgaata
    29161 acaatcttgc caccccttgt atcaaaagga tgtgtgctac agtgtgtaaa atatttccat
    29221 tatcataagt actgtaatgg ataaatccaa agatgattgc tgacaagaaa ctagcgacag
    29281 gaatgctgaa cttactagaa aaaatagcga gaaaatacac tgagaacaat tcttctccaa
    29341 gtatcatgat aggcaaaata ataaataata acgggctgaa agcctcattt attggattgc
    29401 ctgttaaacc ccatttcaaa atttgttgca aaaataccga gactgcggca ctgattacta
    29461 cattgataaa aaaatatttg gctatatttt tgacaggagc gacaggtttc gagaacattt
    29521 ttttcgtatc ttttttacca aaaacaagtg taaatgctat aaatccagtg atagttaaag
    29581 aaaaaatgct aataatggat ggcgcaaata gcaaccctaa caaaccgagt acaacgacaa
    29641 tagtgcccaa tatccctttt ttaagttcta ttttttttcc taaaatgtta ttcatgataa
    29701 taactcctac tat
//
LOCUS       DAYYCW010000023        29659 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000023 DAYYCW010000000
VERSION     DAYYCW010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.023"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..789)
                     /locus_tag="ACHAYA_000978"
     CDS             complement(49..789)
                     /locus_tag="ACHAYA_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604797.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(919..1866)
                     /locus_tag="ACHAYA_000979"
     CDS             complement(919..1866)
                     /locus_tag="ACHAYA_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8604798.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(1995..2759)
                     /locus_tag="ACHAYA_000980"
     CDS             complement(1995..2759)
                     /locus_tag="ACHAYA_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8604799.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            complement(2763..3152)
                     /locus_tag="ACHAYA_000981"
     CDS             complement(2763..3152)
                     /locus_tag="ACHAYA_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604800.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3287..3829
                     /locus_tag="ACHAYA_000982"
     CDS             3287..3829
                     /locus_tag="ACHAYA_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM8604801.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            3977..4609
                     /locus_tag="ACHAYA_000983"
     CDS             3977..4609
                     /locus_tag="ACHAYA_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604802.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(4743..4868)
                     /locus_tag="ACHAYA_000984"
     CDS             complement(4743..4868)
                     /locus_tag="ACHAYA_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM8604803.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5002..5895)
                     /locus_tag="ACHAYA_000985"
     CDS             complement(5002..5895)
                     /locus_tag="ACHAYA_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM8604804.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(6077..7003)
                     /locus_tag="ACHAYA_000986"
     CDS             complement(6077..7003)
                     /locus_tag="ACHAYA_000986"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM8604805.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(7088..7849)
                     /gene="pflA"
                     /locus_tag="ACHAYA_000987"
     CDS             complement(7088..7849)
                     /gene="pflA"
                     /locus_tag="ACHAYA_000987"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM8604806.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(8093..10324)
                     /gene="pflB"
                     /locus_tag="ACHAYA_000988"
     CDS             complement(8093..10324)
                     /gene="pflB"
                     /locus_tag="ACHAYA_000988"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM8604807.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(10601..13051)
                     /gene="parC"
                     /locus_tag="ACHAYA_000989"
     CDS             complement(10601..13051)
                     /gene="parC"
                     /locus_tag="ACHAYA_000989"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM8604808.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(13063..15108)
                     /gene="parE"
                     /locus_tag="ACHAYA_000990"
     CDS             complement(13063..15108)
                     /gene="parE"
                     /locus_tag="ACHAYA_000990"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM8604809.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(15274..15708)
                     /locus_tag="ACHAYA_000991"
     CDS             complement(15274..15708)
                     /locus_tag="ACHAYA_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM8604810.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            15835..16473
                     /gene="plsY"
                     /locus_tag="ACHAYA_000992"
     CDS             15835..16473
                     /gene="plsY"
                     /locus_tag="ACHAYA_000992"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM8604811.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(16605..17480)
                     /locus_tag="ACHAYA_000993"
     CDS             complement(16605..17480)
                     /locus_tag="ACHAYA_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM8604812.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(17562..18353)
                     /gene="codY"
                     /locus_tag="ACHAYA_000994"
     CDS             complement(17562..18353)
                     /gene="codY"
                     /locus_tag="ACHAYA_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM8604813.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(18371..19771)
                     /gene="hslU"
                     /locus_tag="ACHAYA_000995"
     CDS             complement(18371..19771)
                     /gene="hslU"
                     /locus_tag="ACHAYA_000995"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM8604814.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(19785..20333)
                     /gene="hslV"
                     /locus_tag="ACHAYA_000996"
     CDS             complement(19785..20333)
                     /gene="hslV"
                     /locus_tag="ACHAYA_000996"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM8604815.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(20370..21275)
                     /gene="xerC"
                     /locus_tag="ACHAYA_000997"
     CDS             complement(20370..21275)
                     /gene="xerC"
                     /locus_tag="ACHAYA_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM8604816.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(21473..22849)
                     /gene="trmFO"
                     /locus_tag="ACHAYA_000998"
     CDS             complement(21473..22849)
                     /gene="trmFO"
                     /locus_tag="ACHAYA_000998"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM8604817.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(22818..24896)
                     /gene="topA"
                     /locus_tag="ACHAYA_000999"
     CDS             complement(22818..24896)
                     /gene="topA"
                     /locus_tag="ACHAYA_000999"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM8604818.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(25018..25875)
                     /gene="dprA"
                     /locus_tag="ACHAYA_001000"
     CDS             complement(25018..25875)
                     /gene="dprA"
                     /locus_tag="ACHAYA_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM8604819.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(25931..26698)
                     /locus_tag="ACHAYA_001001"
     CDS             complement(25931..26698)
                     /locus_tag="ACHAYA_001001"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM8604820.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(26691..27551)
                     /gene="ylqF"
                     /locus_tag="ACHAYA_001002"
     CDS             complement(26691..27551)
                     /gene="ylqF"
                     /locus_tag="ACHAYA_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM8604821.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            27811..28209
                     /locus_tag="ACHAYA_001003"
     CDS             27811..28209
                     /locus_tag="ACHAYA_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8604822.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            28446..28988
                     /locus_tag="ACHAYA_001004"
     CDS             28446..28988
                     /locus_tag="ACHAYA_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8604823.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(29071..29622)
                     /gene="lepB"
                     /locus_tag="ACHAYA_001005"
     CDS             complement(29071..29622)
                     /gene="lepB"
                     /locus_tag="ACHAYA_001005"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8604824.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
BASE COUNT         7936 a         6255 c         5087 g        10381 t
ORIGIN      
        1 cgcagcgttt cctttccaac tgtagccgaa tataaatttg tatatcactt atttaaaata
       61 gctattttct cccaaagagt ttccttgatc atcgatcaat ccttgtaatg agcgttctaa
      121 gataccatgg ctttttttcg attttttttc ttctgactgc tttgtttcct ctaacgtttt
      181 tgttcgattg aatgttcgta cagacggctc accatttctt ttttcttgat acggcgtcgg
      241 ttctgtatcg aacaacagat aagagtcatc cgtttttttc atcgattcga ttaaatgatc
      301 ctcagaaatt ttgctttctc tagggtctgg gataatcgat gcgggtacat atttaggaac
      361 aaaataagaa cgctcccgca ccttcgtgtc aacagatgcg tttcgtgtag tatgcttgga
      421 ctctgtcctt gtttcacttt gtctgacaga acgagaggaa tcaccaaaaa gtttttttcg
      481 tcctgtcgat gtgatttctg attggtaatg gccggtataa tccggtaatt ttcctttatg
      541 cttttccaaa ttttccttac gcggagctga cttcaatcca tttgttgcac gacgcttgcg
      601 acggattttc tcggtgttcg tgttcgtgaa atgaaattct ttcatctgtt caaacgggga
      661 ctgattctgt tcagtcaaaa gatcttcttg tccgtcaaat aatactcgct tcttgtcgtt
      721 ggttaatttt acgcctaact catcatcgta cgcaggaaaa cggaaatgtt ttcgtctgat
      781 ttctttcatt caaaccatcc ttctcaaaaa tggaactttt ctcaataata gcacagattc
      841 attgttttca gtagcaatgc gctgattatt ttaagaaaat agcgatgaat ttcataaata
      901 tatatatagt aataagactt aattcgttgt ctctctgata actagttcag gaaaaaaacg
      961 acgataacct tgactcgccc ctttttcttc tatttttttt aacaacattt ctccacatac
     1021 ttcacccata cgtataatat cttgcttgat cgttgtcaat ttaggtgaac tgatttggtc
     1081 taaaaatacc ccatcaaatc caatcacacc aaattcttcg ggcaagtgtc ctccagcatt
     1141 tataatccct cttactgctc ctagtgctag ccgatctgaa ctacatacaa aacaagtttt
     1201 atctgtaaaa tctgcccagt tttctgtcat atatctttct acttctgtcg aatgatttgg
     1261 aaaccgcaag attttactag gaagatcaac ttccttcatc acagataaat aaccagtttc
     1321 ccttgaaatt tcaaaaggct cgtccacttc gataccgata taaataatcg tttgataatc
     1381 tctttctaag gcatattgcg ttgcttttgc tgtaccatat tcattatcgc tgtctacata
     1441 atcatatcct tcagaatttt ccccaaacaa gacaactggt ttttccaaac ggtcgatcca
     1501 atcgaagtct ttttttctga cacctgtgat gatgtatcca tcacaagaac cgacgtcgaa
     1561 ggcatcattt gtaactaatt ggagagaata atgcgcttta ccgatccgtt tagcgattcc
     1621 catcagcaaa ttcatgtaat agggctcagt tgtgtcgatt tcttccagta taaataattt
     1681 gatgatctgc gaccgattcg tgacaagtgc ttttgcagca atatttggac ggtaatccag
     1741 ttcagccatt gctgagaaaa ctaactcttt caattcgtca gtcacctgtt ccggatgatt
     1801 gatgactcga gagaccgtca tttttgaaac attggcacgt tttgcaacat ctgctaaagt
     1861 gggcatgctc tcttcctttc taacatgttg tcattcttac tatcaattat accaaatttt
     1921 cctaacagag tttattatag cccaaaataa aaaacggaaa atcctctatc gtcatgagtg
     1981 attttccgtt tcaattattt atactttttt atcaaatttt ttgctaaaaa agtggaagct
     2041 tcttccatta gttgtatttg taccatatgt cgctgtgttt ttgaggaacg aaaaacaatt
     2101 tcttttccgt aagacttatg ccgatttttt tctacaaatc gttccgtttg tatataaggg
     2161 attttttcgt cagcttgtcc atgccaaaag aacagaggac gtccagctaa cttttccggc
     2221 tgcaatgaca aatcatagct cttaacccat gaaatcaaat caaaatagtc ttttggtacg
     2281 acataattat attccgatgc gcgccttacg atttcttttc catattccaa aggtgcaggg
     2341 gaacccatca aacacacgcc cgctactata tgaggatgtt tagccaacaa ggcgcatgtt
     2401 gtcattcccc ccatcgaaac cccgccaact gcaagctgat catgtagaag acctcttttt
     2461 tctaaatgct tgataagtgt gtcaaattca aacagatttc cgtagatact atcccaaaat
     2521 gtataggatg gaatagaaga aaccggctgt ttgcgctccc catgattcat cgcatctggc
     2581 aggatcacac gaatgccctg ctgcgcaatt tttcttcctt gcgtcaaaac taactcctta
     2641 cttgtctgcc atccgtgata atagatgaca acgggaatac gttcctctct taattctttc
     2701 agaacaactt ctaacactgg aatatgatgg acagaacgtt tcaatataat tggattcaat
     2761 tgttactcac ctattttcat taaatttcct gatcgccata gcaaccatgc tccaacagtt
     2821 gtctggaaca ggttcagtgt accaaataat ataaatgcag gactgaatgg ctcgggaaat
     2881 aagcacagag aacaaagtcc caccactcct aatgctagcg aaaggagtgg ataaatagaa
     2941 aaattcaaaa aactggcaac gctaccgaaa atattaccaa taaacagtat caaaaagaaa
     3001 aaaacgctta aaattaaacc atctgtcagc gacatctgaa aaacaatgtt ctctacctca
     3061 attggctgtt gaatcgaata gacacctaag tatatactga gaaggagact tagcacgtgg
     3121 ctggcacaaa acacacgatt gaccgttttc attttaatca cctaacctat ctttataatt
     3181 ctattataat cattttatca tttttttgta tgtataaagt ttcaattttt cttcacttac
     3241 gatttttttt gatattgttg ttatataaat gaaaggagtt ttacaaatga ttgatccaaa
     3301 aatgttgggg atgatcttcc tcattaattt tgcttatatc actctgaaca cgttacgttt
     3361 catgttgaca atgaaaggct atcgactgat tgctccactt gtcagtatgg tcgagatcac
     3421 gatttatatc gtcggcttat cccttgtttt agatcgttta gataatccac tgaatttatt
     3481 tgtctatgcg ttaggttacg cagtaggtat cagtgtcgga attttgattg aagacaaatt
     3541 ggcattagga tacatcatgg tcacaacgat tttgccagca aatagcgcag aagataaaag
     3601 tttgccgggt atactgagag aacacggtta tggtgtgaca cagacttctg caatggggct
     3661 ggaaggtgag cggctagttc tcgaaatact ttctcctaga aaaagcgaac gtgaactgta
     3721 tcgcctgatc aaagaaatcg aagagcgtgc ttttatcatt tcttatgagc caaaatatat
     3781 ttcaggtggt ttctggacta aaaaagtgag aaaacgacaa ggaaaataac tcacaaactt
     3841 aaaaactgtc taatatctaa attaaatttt aaatgttaga cagtttttat ttattatttt
     3901 gattaaaaaa catcgacata tactcatcaa tttgttatta tactttatat acttatcaat
     3961 ttaatttgga gatattatgg aaattcaacg tagaaccact aattttttac atttaggctc
     4021 tttttttgca actttgacga tcttattttt tctatgttat tcatacctta agacctcaca
     4081 tgcgccttct agtgcattag gctggttgct tttggatcat tctgatatca cagaagccga
     4141 agaaaatccg ttttttttga ttttgtcttc tctttgcggc ttactgtttt ctgtttattt
     4201 cggtgcctat ttttttcatc gacatcctgg tacgttaaac gaagcttcaa gcgtattgat
     4261 tgcatttaaa acgttttctt ttttagcaat tcttttttat tcgtttaatc acaatacgct
     4321 tttcttcttt atcatgaatg gtttagtcgt gattttatcc atttttactt attttatttt
     4381 ttcactcatt tttagccaat tgaaatgtcc gctcttcttt cgattagttc ctgtcacctg
     4441 ttttatttac agcttgtttt ccttttttgt ccttgcttta atgccactga ccaacccttc
     4501 gacgatccaa gtcgcaggaa atctgttgga tatgctcttc atcgtcagtc taggtgtatt
     4561 tatgtggcaa agaaaaaaca aacaaaataa aaagcatttt gaagcctgac tattacgact
     4621 tcataaatgc ttttttagtt ttaaacactt caatctaaaa gaataaccca agattggata
     4681 aattattaat gactcgccat tcgtttctta gtgattctgg attatcaaat ggacttccta
     4741 atctaatcag ctagttcttc ccataaaatg ccatacttga ctgaatcata atattgatta
     4801 tttaaaaatc taacttttct tatcactact tcctttgtca ttccacattt ctcaccattt
     4861 ttttgcattc ctttgtttcc agaccatgta gtgaatctaa taggtggtag ataggcaaaa
     4921 tactcaaata attcttttaa ccataaagga agagaaaggc tcctcctgca agagtcagta
     4981 taatttttgc ttactgctct cttaggtttt actcactaaa actgttccat attctttgat
     5041 ataagaaaat gcatgcggta taaatttctt gctcagctca gaaaagattt gtcgtttaag
     5101 aaaggccatt tcctccattg tcaatgaagg atccaatgtt ggtaattcaa tctcaaaatt
     5161 gtttttttcg acaataggta attctaatgt gaatgattct tcttccttta tagtattcgt
     5221 cacaagtaga gactgatgga aaatactagg ccggcgattt ttatatttat tcttacgtat
     5281 cctgtcaacg ataacttcta tttgatcaaa aaagtacgat tcatttacgt ctccaataag
     5341 ttcggctaat tctttgaatg ccttcgttac aggaagctga ttcttctgta caaaaaagta
     5401 attagccaaa gtaagcggga agtgttctat agaggaaagt gtgataaaac ggtacttctc
     5461 atttcctgca gttgtcctca gcaattctgt ctctcggctg tttcttactc cataaaaata
     5521 cgcttctttc cgttcatctt cgaaacaata cgcccaaata acttctccca tcgctaattt
     5581 ttgttcatca acagagagtc tcttcaattg ttcagctatg gtttcagcag ccgaagtggc
     5641 ttgttgcaag taatctttag gatcaaatat tggaaagttc aaatgatatc ttcgttcttc
     5701 cctcttgatt aatcctgcat cgatcaataa atcaagaaaa tgggaaaatt tattttgcga
     5761 aaattctttt ttcaattctc ttagaatcgt ttcttgttct cgatgttcat atagataatc
     5821 ggtcaatgaa gaaaaaagcg gatcatcggt cagtttttga taaaagtttt ccttattttt
     5881 tgaataatag ttcatatatg ttctcttttc tatccgtttt attttactta aaaggctttt
     5941 acgtaaaaag gaggaagaac aagagtcata attgactgtt attctcctcc atttcttcgg
     6001 cctttagtat gttctatgtt tctttttata tcaatcgctt atccattcat tttgaatgct
     6061 tcatactacc atttgattag ctaaatacag ccgtcaattg aggtacgact tgttttttac
     6121 gtgaaacgac acctttaagc aaagcacggt tgttggtcaa agttgtttgg aaagcttctt
     6181 ctactttttc ttttccttta ccagcaacaa gtaattctga atcactgtct aagatgtttg
     6241 tgacaactaa tacgaacaga tcgtagccat ttgctttcat ttcatcttcc attgctgctt
     6301 ctagctctgc ttgtcgatcc atcacttcat taaggtccac tgtattgact tgagcgatac
     6361 gaacgttgga actgcccatt gggaaacttt ttgcatccaa atcaagaaga accgcagctg
     6421 atttatcact tagatttgtt cctgctttca acatttcaag tccataagtg tttagatcga
     6481 cttcagcgat tgctgcaagt tcttttgcag ctgcttcatc ttcaggcgta catgttggtg
     6541 atttgaacaa caatgtatct gaaatgatag ctgagagcat gattcctgcg atttctttag
     6601 gaattgttac ttggttttct ttatatagtt ttaaaataat cgtacttgta catccaactg
     6661 gttctgcacg atagtacaat ggattagctg tttcaaagtt tgcaatacga tggtggtcta
     6721 ctacagctaa aatatttact tcgctgatat ctgaaatact ttgttggaat tcattatgat
     6781 ccacaagcat gacatctgca ttttctgctt tagctgactc tactactcgt ggtgcagata
     6841 gctgaaagtg gtctaatgcg tattgtgttt cttcattagg tgtacctaga gcaactgctt
     6901 ctgtttcttt acctaattca ttttgcaagt gtgaaaacgc aatggctgcc ccgattgcat
     6961 ctgtatctgg attttggtga ccaaaaacta aaatttttga catgtcattc gctcctttgt
     7021 gatttctcct atttaataat agcaaaaaaa cggaaagaag caatacttat cttctttccg
     7081 atgcgaccta tcgcgttaaa tattttgtat actcttctgt atgaagcaat cgtttagcat
     7141 tttcgacacg ttcttttgta ggtggttcga tcccttctaa tggatatttc aaaccaagtg
     7201 tttcccactt gtattttccc atcgtatgat aaggaagaat ctctactctg tctacatttg
     7261 acaaggtttg aataaagttg tctagtcgga tcaaatattc atcataatca cttcgctcag
     7321 gaactagtac atggcgaatc caaaccggtt tgttgatttt tgacaaatat tgagccattt
     7381 ctaaaatatt ttcattgtcc caatgcgtta gttttttatg ctcttcatta tcgatgtgtt
     7441 tgatgtcaaa taacagtaaa tctgtatact tcatcaattc ttgaaaacgt gaaaagaaag
     7501 gttcttcata cgtaaaaggt tttccgcatg tatccaatgt agtatgcatc ttccgctctt
     7561 ttgctttttt aaatagttcg atcaaaaaat cgatctgcaa tagaggttct ccaccactaa
     7621 cagtaatacc acctttatct ccccagtaag gtctgaagcg ttctgcttta tctaaaagtt
     7681 catctgctgt gtattctttc cctccgccaa tattccaagt atctggattg tgacaaaatt
     7741 ggcaacgcat gcggcatcct tgcataaaca cgacaaagcg cagtccaggt ccgtctactg
     7801 aaccgaaagt ttcgatagaa tgaacatagc cgattgtttt ttcttccatt taagttcaca
     7861 tcttttcttt gatttcattt taagtacaag ttagctgtcg aagcgaagct ttctgaacaa
     7921 aatttttcgc aattgccgct tcgttatgtg taaaatttac agggacggaa catacaagtc
     7981 ctgcccctgt aaatcgtttc tttcaaataa gcatactgat tattcgattt tgatgcgatg
     8041 gctattcaac gtcctttcga cgctaagtcg aaaaactttt ttcacgaact ttttacattc
     8101 tgtcgtgtga agttctcatc aagacatcta attgttgttc tcttgtcaag tcacggaatt
     8161 taactgcata accagatacc cggatcgtca agttcggata tttcattgga ttttccattg
     8221 catcacgcaa taaatcatta ctgaatacat tgacattcaa atgataagct ccttttgaga
     8281 agtatccatc taagacatta cgcaagtttt cgattcttgt atcttgttct tttccaagac
     8341 ctgaaggatt gattgtttgg gtatttgaaa ttccatccaa tgcacagccg taattcattt
     8401 tagctgttga gttcaggcta gctaataacc cactttgttc cgcattgtaa cttggatttg
     8461 ctcctggcgc aagtggtttt ccagcttcac ggccatcagg agtatttcct gtatttttac
     8521 cgtaaacaac atttgaagta atcgtcaata atgaagttgt tggttttgag ttgcggtaag
     8581 ttttatgacg tttgatttgt cccatgaagt attccaagat ccatgaaact aattcatctg
     8641 cacggtcatc atcatttccg tatttaggga agtcgccatc cacttcaaaa tcaactgcta
     8701 ttcctttttc attacggatt gcttttactt ttgcatattt gatcgctgac aagctgtctg
     8761 ccgcatgaga gatcccagca atacctgtag caaatgtgcg ttgcaagtca gaatccatca
     8821 aagccagctg agctgcttca taagaatatt tatcatgcat ataatgaatg atgttcaacg
     8881 tattgacata tagttctgct aaccaatcca tcatattcat gaagttatgt ttcacttctt
     8941 caaagtctag tatttctgat gtgattggac gatattctgg tccaacttgg gcgccagtca
     9001 cttcatcgac tccgccattg atggcatata atagtgtttt agccaagtta gcacgagcgc
     9061 caaagaattg catgtctttt ccgacaactg ttcctgatac acagcaagca atcgcacaat
     9121 catcagaacc ccattgttca cgaagctgat tatcattttc gaattgaatc gaagaacttt
     9181 cgatagcgat tctgctagca tatgttctga atccttctgg aaggtgttca gaatacaaaa
     9241 cagtcaagtt tggttctggt gaaggtccca tatttgttaa tgtatgaagc agacggaaat
     9301 cattctttgt aactaatgaa cgaccatcta atcccatacc tgcaatggat aaagtagccc
     9361 agatagggaa tcctgagaat aattggttat attcaggtgt acgcgcgaat ttgaccatac
     9421 gtaatttcat tactaagtgg tcgatcaatt cttgtgcttc tttttctgtg attactccat
     9481 tatccaaatc acgttggata tagatatcaa ggaatgctga gatacgtccg attgacatcg
     9541 cagcaccatt ttgtgatttg attgctgcta aatatccgaa gtatagccat tggacagctt
     9601 cttttgcatt tgcagctggt ttagaaatat catagccata tttcgcagcc atttctttca
     9661 tttgcgctaa tgcacgatat tgttcagtga tttcttcacg taaacgaatc acttcttcac
     9721 tcattgtatg ataaccagtc atatcatgat cttttttctt ttgttccatc aaatagtcga
     9781 ttccatacaa tgccacacga cgatagtcac cgatgatacg tccgcgaccg taagcgtctg
     9841 gaagccctgt aatgattttg tttttacgag cagcacgcat ttcagcagta taagcgtcaa
     9901 atacaccttg attatgtgtt ttgcgccagt cagtgaaaat atgtgtcaat tcatcgtttg
     9961 gtgtatagcc attgcttttt agcgcattgt tagccatatt gattccgccg aacggcatga
    10021 atgcttgttt caaaggtttg tctgtctgta gaccgacgat tttttccaaa tctttatcca
    10081 aatagcctgc atcgtgtgaa gtaatagttg atacgatttc tgtgtccata tcatagacac
    10141 cgttacgctc gaattggata tcatttaact tttgcaattc atcccataat ttttctgttg
    10201 cttctgttgg gccttccaaa aagctgtcat caccgcgata ttcagtgtag ttattttgaa
    10261 taaagtctct agtgtctacg ccttctttcc atttggtacc tttaaatcca ttccattgct
    10321 ccattgtatg gacctcctaa atttctgttt tatgtaacag tttttacgtg attagtataa
    10381 cacagcaccc tatctttgca agggttttct tgttttttat ttaacaattt ttatgtttta
    10441 tttacatata tttaaatcaa cgttgacata ccaacattta agcattgtga acactttcat
    10501 ctttttttgc aaacgtataa ggatttatta aaaatgaatg aaaaagagac gccacctgta
    10561 ttaatacaga tggcgtctct tcattaaaat actacaacag ttacgactcg atgaaaatct
    10621 caggagcatc gataacttcg atgacttctc cacctttttt ctcatccatc gcaaatgatc
    10681 cattcgatgt tcgatcacct atagggaaat cagttgggtc aatcgttaac tgctgccctt
    10741 tttgattcat caaggttatt tcttttgatc ttccttccga catatagata atacggtgcg
    10801 gatttttttt cagctcacgc atgaccgtca ctccccgctt cgcacgtgaa gtttggctga
    10861 tttcttgagc aagcatgcgt ttaacagctc ctcgctgagt aacgattacg atcggcgtat
    10921 caccttctgc taacacaagc agcccgttga taatcctatc tccatctttc aaattcatag
    10981 acttcactcc agctgccttt gctccaacaa cgggaacatc tgttaaagga tagcgcaaac
    11041 ccattccttg atgcgttact aagaaaacat ctaagcctgg ctgatcttct ttcagataca
    11101 cagcaatcag ttcatcttct tccgccttta gcttcataca gctgagcgga cggctcttat
    11161 aggtgcgcca tggttcgaac tctgtcattc gtgtttgctt gatcaatcca ttcttgctga
    11221 tgaatacaaa tgttttctct gcttgaattt tttgatatgg gaaaacagct aaaatcgatt
    11281 cgtctacaga cagattggag attgtttgag aaatgtgctc accagcatct ttccatttca
    11341 aatcgggtag ttcatggaca gggcggtaga tcacatttgc tttatttgtg atcagaagaa
    11401 catggtccaa tgtactcaat tcacctgagt atagcaagta gtcgccttct cgcatcccga
    11461 tttcttctgg tttggaagca ctataagaac gaaggcttgt tcgcttcaca tagccttctc
    11521 gcgtcacact gacaattacg tcttcttgtg ccactaagac ttgcgtatca attttgattt
    11581 cttcgatttc gtcttcgatt gtcgttagac gagcggaaga ataatttttt ttcacttctc
    11641 gcaattcttt tttcataaca gagaaaagct ctttgtcatt tgaaaggatt ttattcaact
    11701 ctgtgatttg cgcaatcaag ctttcggatt ccttttgaag ctctgtgata tccgtgttcg
    11761 tcaaacggta taattgcaat gtgacaattg cttctgcttg ttcttctgta aattgaaaga
    11821 cgtcgactaa attctttttg gcgtcttttt tgtctttact ttcacgaatc gttgcgatta
    11881 cttcatccag aatcgataaa gctttcatca atccttctac gatatgctgt cttttttgag
    11941 ctttggctaa atcgaattga ctacgttttg ttatgacttg ctttcgatgc tcgatataac
    12001 ttcttaaaat gtctttcagt ccaacttgat gcggagtcat atggtcgata gcgaccatat
    12061 tgaaattgta attgatctgt agctcagtgt ttttgaacaa ataattcaaa attccttgag
    12121 cattggcgtc ttttttcaat tcgacgacaa tttgcagacc tgttctatca ctttcatctc
    12181 gtacttcagc tattccatcg attttcttat tcaaacggat ctcatccatt tttttcacca
    12241 aggtcgcttt atttacttca tatgggattt ctgtgatcac gatttgctgt ttattaccct
    12301 tcattgcctc tatctttgtt ttcgaacgaa gaataacttt tcctcgtcct gtttcataag
    12361 cttttttgat ttcatctttt ccttgcaaga tccctcctgt tggaaagtca ggtccaggaa
    12421 tatattccat caatttttca aggctcgctt gtggatgatc gatcatatag attgtgccat
    12481 cgatcacttc agctaagtta tgagtcggga tttctgttgc atatccagca gatatacctg
    12541 tcgatccatt gacgagaagg tttgggtatc gagctggaag gactgttggt tctttttctg
    12601 tatcatcaaa attccacacg agatcgactg tttctttctc gatatctgct agcatctctc
    12661 cacttaactt ggataatctg gcttcagtat aacgcatcgc agcaggtgga tcaccgtcca
    12721 tactcccgtt attcccgtgc atttcgatca gtacttcccg caacttccag tcttggctca
    12781 tgcggaccat cgcttcataa atactgatgt caccatgagg atgataattc cccatgatat
    12841 ttccgacaga ttttgctgat ttacggaatc ctttgtcata cgtattgcca tctttgttca
    12901 tggcaaataa tatccggcgt tgcactggct tcaatccatc acggatatca ggcagtgcac
    12961 gttcttggat aatatatttg gaataacgtc cgaagcggtc acccatcact tcttctaaag
    13021 ttaattcttg tacttcttgt cgattttcca tcaggtgacc tcctattcta cttcaaataa
    13081 ctgattgttt tcatttttat ctgctcgttc ttcatctaat aaatcattag aaactgaagg
    13141 agagatttca gtatcctctt ttttatctaa gatactgcca tcttcttcga gcgtaaattg
    13201 cacgtgattt tcgatccatt ttcttcgagg ttctactttg tcacccatca gtgtggttac
    13261 tcgacgttca gcttgcgcag catcatcgat acggacgcgg atcaacgtac gggaagtcgg
    13321 atccatcgtt gtttcccaca attgttccgc attcatctct ccaagtcctt tatatcgctg
    13381 cagcatatat cctttgccga cttttttaat cattgctgcc aattcgtcat ctgtccacgc
    13441 atattcaatg acttgcttct ttccttgtcc tttggatact ttatataaag gcggtaacgc
    13501 gatatatact ttcccagctt caatcagcgg tttcatataa cgataaaaga aagtaagcag
    13561 taatacttga atatgggcgc cgtctgtatc cgcatcggtc atgatgatta ctttgtcgta
    13621 attacagtct tcgatagaaa actcaggacc gactcctgca ccaatcgtat agatcatcgt
    13681 attgatttct tcatttttca agatatcttg cattttagct ttttcggtat tgatgacttt
    13741 tcctcgtaaa ggtagaattg cttggaactt tctgtctctt ccttgtttag cagatccgcc
    13801 agcagaatcc ccttcgacta aatacaattc atttttctta gggtttcggg attgtgcggg
    13861 tgtcaatttt cctgaaagaa gagattcgcc ttttttacgc ttcttcccat ttcggctttc
    13921 ttctctggct ttacgtgctg cttctctcgc ttctctggct ttgattgctt tacggaccaa
    13981 catctggctc atttcgctat tttcctgcaa ataaaagccc atttgttcac tgatgacgtt
    14041 atctactacg gtacgagcaa ctggcgtacc aagtttttct tttgtttgcc cttcaaattg
    14101 cagaagattt tcaggtacac ggatagaaag tacagcagct aacccttctc tgaaatcact
    14161 accttccaga ttcttgtccc gttctttcag caaaccaact tttctcgcat attcattata
    14221 agatttcgtc atcgccgctt tcataccagc ttcatgggtt cccccatctt ttgtccgaac
    14281 gttattgaca aaagataaaa cattttctga ataaccgtca ttgtattgat aagcgacctc
    14341 tacttcgata ccttcttttt ctcctgaaaa ataaacaact ggtgtcaatg tatctttttc
    14401 ttcattcaga taatcaacaa attctttgat tccttcttcg taatgaaaaa tttccttgac
    14461 tggttcttct ccacgaagat cagaaagaga gattttaacg ccttttagaa gaaaggcaga
    14521 ttctcttaac cgttcggcta aaatttcata tgaaaatttc gttgtactga atattgtatc
    14581 atctggtaaa aaatggacag tcgtcccgtt tgcttttttt gtttttccta tctttttcaa
    14641 agtaccgtca ggtttaccgc cgtttttaaa tttctgctga tactctacgc catctctaac
    14701 gatcgttaca attagccatt tcgataaggc atttaccaca ctggctccta caccgtgcag
    14761 tccaccagaa gttttgtacc ctccttgacc aaactttccg ccagcatgca gcacagtaaa
    14821 aatcacttca actgttggta ttccggaagc atgcattcct acaggcatcc cacgtccgct
    14881 atctgcaaca gttatactat tgtcttcatg gatggtcaca tcaatctcac tgccataacc
    14941 tgataatgct tcgtcaactg cattatcgac gatttcatag actaaatgat gcaagcctcg
    15001 gctgtcagtg gatccaatgt acatccctgg tctttttcta actgcctcta atccttccaa
    15061 tacttggatg gacgagtcat tgtattcgtt gtttactttt tttgccaagg caaacgctcc
    15121 ttcaataata ctttgcggca aaaccgcacc acgattatca tactagaaaa ggtcgtgaaa
    15181 aaaaagaggg tagacgagaa atcaatttta attatctcct aaccgctttt tcgacattcc
    15241 cctctatcga tcatctcttc tttgtggtca tttttatttt tggttttctt tcaacttacc
    15301 aagttcaatc ttaatacaac gatccattac gatatctgta cgtcctgctt ttcttagcat
    15361 ctcagctgct tctttgtttt caatccctaa ttgtgcccag aacactttag catcagtttt
    15421 caaaaaatca cctgctattt caggtaaaaa ttcactccgt ctaaatacat ctacgatatc
    15481 gattggatca tcgatttctg tcaaattcgc atagactttc tcgcctagaa tttcttcacc
    15541 agccaataat ggatttactg gtatgatttt atatccattg tcttgcaata cctgtgcaac
    15601 catgtaactt gttcgttctg gtttattgct caagcctacg acggcaatat tttttgcttc
    15661 ttccaaatag ttgaaaatcg tttcttgtgt tggatttttt acagacattt ctgccacccc
    15721 ttgcttttct ttttattcca tctattatac ttgtatcttt cattttttca attgttcctg
    15781 ttacctattc atgctaaaat aaaggatagt tttcaacttg aaagagggta aaaaatgaaa
    15841 ttgatcttat tacttatttt agcatatctt ttaggttcta ttccttccgg tgtctggatt
    15901 ggcaaattgt ttttcaaaaa agatatccga caacacggga gcggtaatac tggaacgacc
    15961 aatacatttc gtgtattagg caaaacagcc gggattgttg tcctgcttat ggatatttta
    16021 aaaggtacat tggctacttg tttgccgatg atcttccacc tttctgggat taaccctctt
    16081 tggtttgggg tatgcgccat tcttggccat acatttccga tttttgctgg gttcaaagga
    16141 ggaaaagctg ttgcgacaag tgcaggaatg cttttaggat ttaatcctgt attttttgtt
    16201 tattcttgta tcatctttat cgttagttta ttttgtacaa gtatggtgag tttgagcagt
    16261 atgatcagtg cagtattgat cactttatct acgatcgtcc ttccttatgt agctcctgtg
    16321 attttggcta agcccaattg gttactgacg atcattgcct ttttagtttc ttcctttatc
    16381 ttttatcggc ataaagacaa tatcaagcgt atcagaaacc atactgaaag tcgaatcccg
    16441 tttggtttga atagcagcaa gaaaaaatcc tgatactttt tcattccaaa gagataaaac
    16501 gggagtgtga caaatcgatg tcacactccc ttattactta caggatagct tttttacaca
    16561 ccttggtaaa cggtgttccc tgctaacttc tcggtcacaa cctcttactt cactgtgatg
    16621 gaatattttg tcttgaagat ttcatgggca cctagttgat tgatccctac tttttccatc
    16681 aagtttccac tactgttcac cgtgtcagcg attccgcacc acggttcgat acaaacgaaa
    16741 ggcgcttctt ttggataagg agaccagatc cctacataag gcatatcttt atagcttaaa
    16801 gttacgctat gggaagattt ttcacttcga atggcgtacg catttagacc ccttgtttca
    16861 aaaaccaagg catcttgctc aaaaagttca cgattcaaag ccagattcgt atttgtctga
    16921 cctaacgtct tttgatccat atcgatgaat gggcctttca atggtaggcg taatcttgat
    16981 ttcaatggag aaaatgccag atagaaatct tcaaatgaca atccttcttc aagaggtaca
    17041 ttgaaagcag gatgtccacc gatagaaaag aacatttctt tttcaccggt atttgccact
    17101 tgataatgaa ccgtgatgcc gtctcctcct aattcatagc tgatcactaa ttcaaaatca
    17161 aatggatact tttctaatgt ctccggcgta ctccttaaga tgaaactagc catttcttgt
    17221 ccatgttcaa tgacttcaaa atccatatcc cgagcaaatc cgtgttgccc catttgatat
    17281 gttttccctt gataaacata ctgatcatct tttaaacgtc caacaaaagg gaataaaacc
    17341 ggtgcatgtc tgccccaata cgctggatca ccttgccaga tatactctaa accatgttct
    17401 tttgatttca gactgatcaa ttccgcaccg tgttcagcga tttcggcgat cagctggtca
    17461 ttttgaataa taacagtcat tgccattcct ccaacttcga cttacttttt aaaatgggaa
    17521 ggctggaaca aacatttcta tctgccccag tccccttact tctaataact gtgttttgct
    17581 aactcgtctt taaaacggga attcaatact tttaaataag ttcccttcat gcctaacgaa
    17641 cgagattcaa tgattccagc tgattccaat tttcgtaaag cattgacgat cacagaacga
    17701 gtgatgccga tactatcggc aatattagaa gcagtcagac gaccttcatc tccatccaat
    17761 gcttcaaaga tagcttgtac agccttcaat tcgctataag ataatgtgtt gattgccatt
    17821 tggacagccg ttgcactccg tacatctgct tcgatgcttc gagacttctg ataaaggatc
    17881 tgcataccga caaccgtagc tccatattct gctaagacta gatcgtcatc tgcaaacgat
    17941 tgttccatac gagataagat aatcgtaccc aaacgttcgc cagctccaaa gatcggaacg
    18001 acagtcgtca gaccaaatgg atatttctct cttgactcta ccggaaatgc agtaatatca
    18061 ctagtaattg ggatattagc ttcagttttc atcaactgtg ccactgtatc agtataagat
    18121 tcaggaaatt gtttctcaac aaacattgtt ttgattcgtt cattgttcac atcgtgacgt
    18181 tcattataac ctagtaatac tccgtcactt cctatgatat aggtgttgct gtcaagaata
    18241 ccacctagtg tgacagccat tttatcgtaa ggcaaatctg cttgcatgtc aaaagtattc
    18301 ttttgctgca aaagtgtgtt tatcttgcgg gttttcgtta gcaagtctgt cattgtcgtc
    18361 ctccgatttt ttataaaata tagcgactta aatcttcatt tgccgcaatt gagccaagtt
    18421 tgtcatccac atatgcttct gtgatcgtta tttctcccat ttgcatatct ggtgcttcaa
    18481 ataatagatc ttctagtagt ttttccaaaa tggtatgcag tctgcgtgcg ccgatattat
    18541 ctgtgtcacg gttgacatcg aaagcgattt gagcgatccg ttcaattgct tcttttgtaa
    18601 acgtaactga cacattttct gtaccgatca acgcgacgta ttgttttact aaagcattat
    18661 ttggttcggt cagaatcttc acaaaatctt cagctgtcaa atcatctaat tctacccgta
    18721 tcgggaagcg tccttgaagt tctggaatta gatcacttgg tttagacaaa tggaaagctc
    18781 ctgaagcaat gaataggacg tgatctgttt ggatgatccc gtattttgtg tttacttgtg
    18841 aaccttcgac aattggcaat atgtctcttt ggacgccttc tctggatact tctccagatt
    18901 gctgtgattt agaagtgatc ttatcgattt catcgataaa aataattcca ctttcttccg
    18961 ctaatttgat tgcctcacta tggatatcag catccttgac taattttgct gattcttcct
    19021 tgatcaataa ctctaaagct tctttgactg ttaccgtccg ttccactttt ttcttcggtg
    19081 tcaaagcacc tagagtttca tttaggtcga tccccatttg ctccaatcca ttgttcatca
    19141 ttggtgcagg tttcttttgg tcttcaactt caatggttac ttcacgacta tccagtaaac
    19201 ccttttgtag ttgttccaag actgtacggc gattgattcg aatatcttct gtcacttctt
    19261 cttgcggttc agcggtattt tgctgactag cattaaaaag attcatcatt tgttcaaatt
    19321 gattagtgga ttgtttctgt tcttttttga taccaggtac caagattttt accaaacgtt
    19381 tttctgcatt ttttttagca cttgtgtata cacggctgta ctgctctttt tctacgattt
    19441 ggatcgcatt ttctactaaa tcccgaacca ttgattctac atctcttccg acataaccga
    19501 cttcagtaaa ttttgttgct tcgactttga caaatggtgc attgacgatt tttgccaaac
    19561 gtcgtgcgat ttctgtttta ccgacccctg taggtccgat catcagcata ttttttggtg
    19621 taatatcttg ttgcattttt tcatctaact gcaaacggcg atagcggtta cgcaaagcaa
    19681 cggcaacaga tttttttgct gcgtgctgtc ctacgatata ttcatctaat tcacttacga
    19741 tttgtcttgg tgtttgtttt aattcataca tatctactca cctcttacag ttcttctacg
    19801 ataatattat gattagtaaa gatacagata tctgcggcaa tatttaacgc attttctgca
    19861 atttctttcg cactcatgtc gttttgagca tagtttttca ttgctcgggc tgcagccaac
    19921 gcaaaattcc ctcctgatcc gatagctaag ataccatcat ctggtgtgat aacttctcct
    19981 gtacctgaaa caagaagcat ttctttatca ttcattacga tcagcatcgc ttcaagtttt
    20041 tgcatagatt gctgtgtccg ccattcttga gctaattcga cagctgcgcg ttgtagattc
    20101 ccgttatatt cgtttagttt gccttcaaat ttttcttcta aagtaaacgc atctgcaaca
    20161 cttccggcga aacctacaac aacttctcca ttgtagatac gtcgtacttt ctttgcagtg
    20221 cctttcatga caacagattc acccattgtt acttggccat ctccagccat agcaaatttc
    20281 ccgtcttttt cgacggcaca gatagtggtt gaatgaaatg gtgattcgac catgtttgtt
    20341 tcctcctaaa tttgttcttt tccttatttt tacgctcttg gatggaaaga acgatagttt
    20401 ttttgtaaac tttcttttgt tacatgggca tagatctggg tagtcgataa attggcatgt
    20461 cctagcaatt cttggactgt ccgcaaatct gccccattat ttaataaatg agtagcaaat
    20521 gtatggcgca gcatgtgagg atggatatca ctatccaaac tgcttttttt gatcatttga
    20581 ttcaagacat attcaattcc agtaggcgtg attggttttc catgatgatt cacaaaaacg
    20641 atatcatgct cttcgtgata ttttgtcatc aatacctggc ggccttccct gaaatattct
    20701 tgcagagcat cttgagcaaa agaacctaga ggagcatatc tctctttgtt ccctttacca
    20761 tggatcaaca tgacctctga atcaaaatca atgtctgata ccgacaaact gctacactca
    20821 cttaaacgga tgcctgaccc gtaaaggact tccagtaaag cgcggttcct caaatccaaa
    20881 ggactatctc ctgctacgct gtcaaaaagt gcttgcattt cattttcata aaaaaaacgt
    20941 ggcagtctca aatttttctt tttgagatga acgtaagaaa atggattttc ttttatcact
    21001 tcttctttga gaagatactg ataaaacgaa cgtaggctgg ctatcttgcg actgatagaa
    21061 tttctgctat agctaggacg atgatcagct aatgcactca aatagacacg aaccgtaaaa
    21121 tattccgcct tcagcagatc agcgtctcca gattcctcca aaaaatgttt gaaatggaga
    21181 atgtcttctt catatgcttc tttcgttttt tctgaatatc ctctttcgac aatcaaataa
    21241 cgcaaaaatt tttgttccca cgtggcttct gtcattttct caccttcctt ttgtcccaga
    21301 gaataattta gcacagcgtt tataaaaaga caaataaatt gtcagaattt aaccaatatt
    21361 tctaagtttg ttcacaattg gcgcaattcc tcacattttg tttgagaaat tcttaagaaa
    21421 aacgcaagaa aaagagcgag actatccgtc tcaccctctt catggagttt ccttactcat
    21481 ttgggattgc agttttttct aattctgttt ttattgcctc attagctttt aaagctcgtt
    21541 gtgcaattgc ttcatatctt gcttttttgt ctcgaatgcg ctctggcagt tcaggaaaca
    21601 gaccatagtt ggcattcatc ggttggaaat gttttccttc cgcatgagta atgtagtaag
    21661 ccatacttcc aatagctgtt tcttgtggaa actcgactgg ttccatttct tttgctaagc
    21721 gtgcggcgtt gattccagca agtagtccgc tcgctgcact ttcaacatag ccttctacac
    21781 cagtcatctg acctgcaaag aacagatctt cgcgtttcgt tgattggtaa gtcggtttca
    21841 gcaattctgg cgaattcata aagctgtttc ggtgcatcac accatagcga acgaattcag
    21901 cattttctaa cccaggaatc atccggaaaa cacgtttttg ctcgccccat ttcaagtgag
    21961 tctgaaagcc cacgatgttg tataaagaag ctgcagcatt gtcttgacgc agctggataa
    22021 cagcataagg acgtttgcca gttttaggat cttccaatcc aactggtttc ataggtccga
    22081 aaagcatcgt tttgaatcct cgctgtgcca ttacttcaat tggcatacat ccttcaaaat
    22141 atttttcttt ctcaaaagat ttaagttctg ccacttcggc attgattagt gcctcatgga
    22201 aagctttgaa ttcctcttct gtcatcggac agttcaagta agctgcttct cctttgtcat
    22261 agcgggactt caaatatact ttatccatat cgatcgtagc tttgtctacg attggtgcag
    22321 ctgcatcata gaaataaaaa ccatctgaac cattgaatgc tttgatgctt tctgctaatg
    22381 gatgagaagt caaaggtcct gttgctacta cggtgattcc ttccggtatt tcagtaattt
    22441 cttcgtttat cactgttact aatggatgct ctttgacttt tcgggtaatt gtttcagaaa
    22501 aagaatcgcg gtcaacagct aaggcaccac cagctggcac tgctgtttca tctgccgaag
    22561 tgattactac cgaattcaat cggcgcattt cttctttcaa taaccctact gcattagcca
    22621 aactgtttcc tcttaatgaa ttggaacata ctagttcagc aaaattactt gtgtgatgcg
    22681 cctcagtcga tttgactgga cgcatttcat ataaacggac agggactcct gcttgtgcga
    22741 tctgccaagc agcttcactt ccggcaagcc ctgctccaac aacatttacc attttcaatg
    22801 aaagaacctc ttttctatta tttttggatg tcttcttcgt aatctccatt tacacagact
    22861 acttgtttac cgcctttgac tttcttctcg accagatatc cgtcacattt tggacaattt
    22921 ctgcccactg gtttatccca agaagtgaat tcacattctg ggaaacggga acaaccatag
    22981 aatagtctgt ttttcttaga ttttcgttca atgacttgtc cttcatgaca tactgggcaa
    23041 gtcacaccga tttcttttac gattggtttc gtattccgac aatcaggaaa attactgcaa
    23101 gcataaaact tcccatattt ccctaatttt atcaccatcg gatgtccaca gacatcacaa
    23161 tcgaatcctg ctggttcatc tttgatttga attttttcga ttttttcttc cgcattcgtc
    23221 agttcttttt caaacggttt atagaaacga tcaaccactt tgacccacgc ttccgtgcct
    23281 tcttcaattt tatccaaatc tccttccatt tctgctgtga aatggatatc gacgatttga
    23341 gggaagaatt cacagattaa tgaattgaca atttctccta gttcagtcgg ctcaaatctt
    23401 ttttgtgtca attttacata gtagcgtctt tggatcgtat caagtgttgg tgcataagtg
    23461 gaaggtcgcc ctaccccgtt ttcttctagt gcacgaatca acgtagcctc actaaaacgt
    23521 gctggtggtt gagtaaaatg ttgttttggt tcaatatcaa cagattggac tacatcgcct
    23581 tcttcaagtt ctggaagaat attttctttc tcttctttgc cgtcatctct accttccaca
    23641 tacacttgca tgaatccttt gaatttgatt tttgaaccgt tcgcaatgaa gatgacacca
    23701 ttttgttcta atgtcacttt cattgtatct aaaatagctg gtgtcatctg actagccaca
    23761 aaacgtgacc agatcaatgt ataaagtttc aattgatctt tatctaaata ttgtttgagt
    23821 tcatctggtg tacgaagcac actcgttgga cgcacagctt catgggcatc ttgtgcgcct
    23881 tgagcatttt tgatctttcg gtgatcatgt ctagaaaatt cttttccata cgtatcttca
    23941 ataaatgctg cggcctctgc ttttgctgta tcggcaatcc gggtagaatc agtacgcatg
    24001 tatgtaatca agccgactgt tccttgttta cccaaagtga ttccttcata taattgctgt
    24061 gctaccatca tggtttttct tgttcgaaag tttaacttac gggcagcttc ttgttgcagg
    24121 ctacttgtag tgaaaggatt cgcagggttt cttttgcgct ctttcttttc tactttttta
    24181 acttcgaagt ctgttccatc aattctagca gtgacttctt ttacactttg tgcatctggt
    24241 aatttctttt tcttaccgtc gattccccag aaatttgctt tgaatttctt gcgagctttt
    24301 ttaaaattac cgtcgatgct ccaatattct tctggaacaa attctcggat ctctttttca
    24361 cggtcaataa taatttttaa agcgacagat tggacacgtc ctgcacttaa ccctttcttg
    24421 accttacgcc agagaatagg actgatcgaa taaccaacga ttctgtctaa ggtacgacga
    24481 gcttgctgtg catctactaa atctacatcg atcgttcttg gttccttaaa agctgctttg
    24541 actgcgtctt ttgtgatttc attgaagacc acccgatttt tttctttcag atccaaccca
    24601 agtaggtacg ctaaatgcca agcaatcgct tctccttctc tatccgggtc acttgccaag
    24661 taaacttttt gagctttttt tgctgcggct ttcaggcttt tgatgacatc gccttttccg
    24721 cgtatagaaa tataatgggg ttcataattg ttttcaaaat cgatacccat tttacttttc
    24781 ggtaaatctc ttatatggcc aacactggcc attactttat agtttcgtcc aagatatttc
    24841 tcaattgtct tggcttttgc aggtgattct acaatcacta aatatttata cgccattact
    24901 gaatggactc ctttccattg tcgtgttcct actattaaaa gaggattcat acaaatcgta
    24961 gctaatcata tccattcaga aaacgtttgt caagaaaagc aaatgaaaag tatggtgtta
    25021 ttctttaaat tctgccaaga tatcttcacc cgaaaccgtg caaactgcac cgtcttggat
    25081 caaatgatga catccggtag aatgttctgt taaaatattt cctggaacag aaaaaactgt
    25141 tcttccaaga tccaatgctt gctgtgctgt tatcagagag ccgctttttt tcttggcttc
    25201 aaagacacat gttcctttag acaaagctgc gataatccga tttctcattg gaaaacaatg
    25261 ctttttcggg ccggattgag gaggatattc actcaaaaga agatgatcat ttgcaatcac
    25321 ctcttgcaag tcacgatttt cagcaggata gacttgctct atccctgttc caatgactgc
    25381 gattgtccgt ccaccatgag agattgctgc ttggtgaccg tatgtatcta tccctttcgc
    25441 gcagccactg acgatcacat atccagcttc tatcactggt tttagtaaat aataaactgt
    25501 ttttttggct aaagcagaag tgtgtcttcc accaacaacg cttaacatgt tacaagaaag
    25561 taaagataga tcaccattat aaaacagtac aattggcgga taggtcaagt gaagcagctg
    25621 agggggatat ttgggatcaa agcaagtgat aaacgactgt ttcgattgga ttttgcccca
    25681 gttagcgtga atttcctgcc aacttttgcg aaacagctcc cggtgtttgg ttattttacc
    25741 tatcttaatg atgtcttcaa atccgatttc tttataagga tactgcatca tccagttagc
    25801 aagtgcccat tttccttgac tactgattcc ttttgtataa gcaagtttca ataaattttc
    25861 ttctatttga tacatcataa aaccgccttt cgtctctctt tctaataaga tacgtaaaag
    25921 cggctgtttt ttattgtaaa tattttttta caggtgcaaa acttttacga tggattggcg
    25981 taattccttg tcgttcaagt ccttctaagt gttcctttgt tccataacct gcattatgcg
    26041 agaaaccata tccaggataa agcacgtcat attctttcat caattggtct cgatatactt
    26101 tagcaatgat acttgcagca gcaatcgaca cagatcgtgc atcccctttg attatttttt
    26161 cctgctgaat aggcaaatcc aaggtcatcg catcgatcaa caaatagctt ggtacaatgg
    26221 acaatttttc tactgcttgg atcatcgcta atttacttgc ttcataaata ttcacatcat
    26281 cgattgtctg ctgatcaacg atcccgatcc cgattgccag ggctttttct tgaacttgtt
    26341 gataaagaag ttctcgcttt ttctctgata attgtttgga gtcattcagc cctaatatga
    26401 tctcattttt cggcaagata actgcagctg ccacgacagg tccagctaat ggtcctcttc
    26461 ctacttcatc aatgccggca atccattggt gaccttgtgc gtacccttgc ttttcaaagc
    26521 aaaacatttc ttcatatttt tccttaatta atgcttcctt tttcaatcgt cgttcaaaag
    26581 aagccagtgc ttgctgcacg cctttgcgtg gatcatttct taatgattct agtgtaggat
    26641 catctattct ctgtatgctt ttcaatcgtt ctttgatact ttggatggat tcagtcatca
    26701 cttttttgct ccatttcttc gcaacgatcc agtgtgtatc gtccaagttt tccttgacga
    26761 atatcatgca cgactaattc gctgccacga tcatagtctt ctttataccc acgttttgct
    26821 gtaatatcca tcagtaattc cggtgccaat ttttcttctt gctcgattga taaaccataa
    26881 cgatcgctga ttcttcctga ataatatcgg gcaaaaaagt ctaatccgta aatcgctaaa
    26941 tcatctaaat ggacaagctg gtctttgatc gcgcctgtca gcgctagttt tttgccgatt
    27001 tcttcatctt caaatttggg ccataaaatc cctggtgtgt ctaacaattc caactcatta
    27061 cctaaacgaa gccactgctg accttttgtt acgccaggct tatttcctgt ctgtgcaatt
    27121 ttcttgccta ctagtcgatt catcaacgta gatttcccaa catttggaat acctaagacc
    27181 attgcccgaa tagcgcgagg cttgatcccc cgagcagcat ctcttgcccg tttttctttc
    27241 aatgcttctt tagccgcagg tactagacct ttaattcctt tactttcttg tgccgtgata
    27301 gctaatgcaa gatggccttg tttacgaaaa tattcttgcc acagctttgt ctgttgtgga
    27361 tctgccagat ccgctttatt caataaaaca aggcgaggtt tttgctgaat gatctgatct
    27421 agcattggat tacgagaaga taaaggtaat cgtgcgtcta ccaattcaaa tacgatgtcc
    27481 acatatttca ttttttcaga aacttctcgt cgggcttttg ccatatgtcc cggaaaccat
    27541 tggatggtca ttgttctctc cattcctttc ataccggaat attccccggc tttacttgtt
    27601 tttttctttt tcaatataaa ttcacttttt tattataaag aaattccatc tattagttaa
    27661 gtgaaagtga accagtcctc attctatcac atcttcactt ttttgatgat gcaaattttt
    27721 attttggttt ctatatatga atctaaattt ttgacagcgt ttcctaaata agagatgata
    27781 gacgaacaaa acagaaagga gacgaaacaa atgaatagta tggtttttgt taattttcct
    27841 gttgctgatg tcaatcgttc cgttgctttt tatgaaaagc tgggttttaa aaaaaatgaa
    27901 gaattttcta ctgaagaagc tggttcaatg gtatgggatg acactttttg gataatgctt
    27961 ctctctcatg atttttataa aaaatttata aaagataaag aaatagctga taataaaaaa
    28021 acaagtggtg ctttgatttc gtttagtgtt gaaaatgcgg atgaagtaaa aaaatttgct
    28081 caaattgcaa aagaaaatgg tggtactttc cacgaagtag atatgggaat gcctgaagaa
    28141 gaaatgtttt cattagaagt ccaagatcta gacggaaata cattagaacc cgtatggatg
    28201 aagatgtaag cagataacta atctgacgtt ttttataaca aaaaaacaaa ggcggatgat
    28261 acgataagta ttctccgcct ctattttttt tattcgtaca atctcttttt tacagtttat
    28321 aaaagcagtc ttctcaccct ttttttaatt gttagatgcc agtgtatgta aatcaccaat
    28381 gtgtcatgac acattggtga ttttatggta tccttttatt attaactgac aaaggagaga
    28441 atattttgaa cactttgatt gcaaaagacg tttttagatt gcccgaaatg aatgcaagat
    28501 tgttgtcgac ctctgctctt ttattagccc ttgatgtcat cttatacaag atagcgattg
    28561 gtcctgctta ttttcaaatc acattcggat ttttaagtat ggcgcttatg ggttatctat
    28621 atggaccgat ctgggctggt ttgcttgaaa tgttgtcgaa cactttaaat ttcacgatat
    28681 ttggttcagg aacgtttcaa gctgcttttc ttatcacggc tttccttggt ggatttatta
    28741 atggcttatt tttatatcat aaaaagataa acctgccttc cgtcatcgct gcccaattat
    28801 tgattatgat cccagtcagc ctttttatca attcttggct gatgtctgtt ttgtttggtg
    28861 cagatttcaa agtaattttt ctaggtagat tcatcaggaa tatgatccaa atccctattc
    28921 aaataatggt tgtttattca tttatcaaaa ctttagaggc tagaaaagtg tttaaacgag
    28981 ctgtttgatt ttacgtacta aggtagtaaa ctgattttgg attaaacaat aaagcgtgca
    29041 gtctcaacag actgcacgct ttattgtttc tcactggatc cattttattt catcaagtgg
    29101 ataatagaca aatcttgctt tacctagaat atcagtttta ttgatcgtgc caaacgaacg
    29161 actatctttt gaaactcttc gattatctcc aagtacaaaa taattatctt ccggaagctt
    29221 ggattgtccc gttagctctt gtagtgtaaa gtccgtcgta taggatgcat ttgcatgatc
    29281 tgatttcagg tttttagtta gataaggttc cttaactact ttcccattga tcttcaattg
    29341 atcattttgg tagctgacat tttctcctgg aagaccgatt actcgtttga tataaatcgt
    29401 gccatctgct aattgaaaaa caacaatatc aaatcgtctg atctccgaaa atttttccat
    29461 cacaaccata tcaccttgct tcaacacgtt ttccattgaa ttgccttcca ctggtaccgg
    29521 gatgaataaa aatccccgca acatgaacgc aaggaataca gaaacaagta agtatttgaa
    29581 tacaagccag aagcgattaa tatagcgttg tttttttgtc aatctctatc aatccatccc
    29641 tctttttttc attctttct
//
LOCUS       DAYYCW010000024        28091 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000024 DAYYCW010000000
VERSION     DAYYCW010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.024"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(206..1105)
                     /locus_tag="ACHAYA_001006"
     CDS             complement(206..1105)
                     /locus_tag="ACHAYA_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8604825.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1115..1471)
                     /locus_tag="ACHAYA_001007"
     CDS             complement(1115..1471)
                     /locus_tag="ACHAYA_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8604826.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1826..2122
                     /locus_tag="ACHAYA_001008"
     CDS             1826..2122
                     /locus_tag="ACHAYA_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM8604827.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2766..3719)
                     /locus_tag="ACHAYA_001009"
     CDS             complement(2766..3719)
                     /locus_tag="ACHAYA_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8604828.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3742..4497)
                     /locus_tag="ACHAYA_001010"
     CDS             complement(3742..4497)
                     /locus_tag="ACHAYA_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8604829.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4494..5453)
                     /locus_tag="ACHAYA_001011"
     CDS             complement(4494..5453)
                     /locus_tag="ACHAYA_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8604830.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5446..6396)
                     /locus_tag="ACHAYA_001012"
     CDS             complement(5446..6396)
                     /locus_tag="ACHAYA_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8604831.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6546..6998
                     /locus_tag="ACHAYA_001013"
     CDS             6546..6998
                     /locus_tag="ACHAYA_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM8604832.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7087..9354)
                     /gene="gshAB"
                     /locus_tag="ACHAYA_001014"
     CDS             complement(7087..9354)
                     /gene="gshAB"
                     /locus_tag="ACHAYA_001014"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM8604833.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9541..10539)
                     /gene="ftsY"
                     /locus_tag="ACHAYA_001015"
     CDS             complement(9541..10539)
                     /gene="ftsY"
                     /locus_tag="ACHAYA_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM8604834.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10550..11359)
                     /locus_tag="ACHAYA_001016"
     CDS             complement(10550..11359)
                     /locus_tag="ACHAYA_001016"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8604835.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11368..14949)
                     /gene="smc"
                     /locus_tag="ACHAYA_001017"
     CDS             complement(11368..14949)
                     /gene="smc"
                     /locus_tag="ACHAYA_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM8604836.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14969..15655)
                     /gene="rnc"
                     /locus_tag="ACHAYA_001018"
     CDS             complement(14969..15655)
                     /gene="rnc"
                     /locus_tag="ACHAYA_001018"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM8604837.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(16003..17784)
                     /locus_tag="ACHAYA_001019"
     CDS             complement(16003..17784)
                     /locus_tag="ACHAYA_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8604838.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17810..18724)
                     /locus_tag="ACHAYA_001020"
     CDS             complement(17810..18724)
                     /locus_tag="ACHAYA_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8604839.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18751..19713)
                     /gene="opp4B"
                     /locus_tag="ACHAYA_001021"
     CDS             complement(18751..19713)
                     /gene="opp4B"
                     /locus_tag="ACHAYA_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8604840.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19713..20657)
                     /locus_tag="ACHAYA_001022"
     CDS             complement(19713..20657)
                     /locus_tag="ACHAYA_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8604841.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20658..21662)
                     /locus_tag="ACHAYA_001023"
     CDS             complement(20658..21662)
                     /locus_tag="ACHAYA_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8604842.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(21930..22172)
                     /gene="acpP"
                     /locus_tag="ACHAYA_001024"
     CDS             complement(21930..22172)
                     /gene="acpP"
                     /locus_tag="ACHAYA_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8604843.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(22294..23295)
                     /gene="plsX"
                     /locus_tag="ACHAYA_001025"
     CDS             complement(22294..23295)
                     /gene="plsX"
                     /locus_tag="ACHAYA_001025"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM8604844.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(23344..25380)
                     /gene="recG"
                     /locus_tag="ACHAYA_001026"
     CDS             complement(23344..25380)
                     /gene="recG"
                     /locus_tag="ACHAYA_001026"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM8604845.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEGSEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(25497..27179)
                     /locus_tag="ACHAYA_001027"
     CDS             complement(25497..27179)
                     /locus_tag="ACHAYA_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM8604846.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(27231..27593)
                     /locus_tag="ACHAYA_001028"
     CDS             complement(27231..27593)
                     /locus_tag="ACHAYA_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8604847.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            27863..28051
                     /gene="rpmB"
                     /locus_tag="ACHAYA_001029"
     CDS             27863..28051
                     /gene="rpmB"
                     /locus_tag="ACHAYA_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM8604848.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7767 a         6080 c         4950 g         9294 t
ORIGIN      
        1 ttatattttt ttttgtgatt atcgttcaaa aaaagaatac acgcttgcat ctagtactct
       61 gcgagttaca aatcatccga tcaaataaac attcgctgat tattttccgc aaaaaaagca
      121 gcatatccac gataaatgga tctactgctc ttacttatcc ttatgtttta caaggtattt
      181 tcttttacac attactaatg ctgtctcaat acggaacgat tgtcatcgct tgttcaaatt
      241 cctcttgcaa atgttgtgtt tgataggcct gaaaagctgg gtgatgggca cgcaagagga
      301 cacgtaattt cataggatca tagctgtcta gcaactgttc tttcaagaca aaatctcggg
      361 tcagctgcca ttcataataa taaccaaaag gtctgcagct gtaaatcgtg tgaccgatgg
      421 atttatcttc aaagcgacga tgcgtatggc caaaaactac ttgtttgata tttgaataac
      481 ggtcaagtaa ttctccgaat gattctgatc ctaaaaatgc atttaatttg ttccaacgct
      541 cataaggagc agattgatac atgataaatg cccgtttagg aacgaaatga gtggataaaa
      601 tcacctcaag tttttttgct tccagctggt ctagcaaatt ttttgcagct gcaagcattt
      661 ctgcattgac ctcgagatct gttttcccac gctggatgaa ccgatcatac caatacaaac
      721 gcttcattcg tacgattgcc tcgtaatctt tcaattcgga aaactgatag tcgtaccagc
      781 cattcatccc taaaagaacg gtctgctcat ttaaagcaat ataccgttca tttaaaaaat
      841 gtgggtctgg aaaatggtcc atcatctgtt cattgggcac atctgccaac tcgtgattgc
      901 cgaaatgaaa tgtcgtagat aatcctttat tgacgaaaaa ttcgacaaca gctaggcaac
      961 ggtctacttt attagccgta tctccggcta gatgaagccg agtgatcttg tgcttttgca
     1021 aaacttgcca aagctgcatc agctctgatt cgccgaattt attgatgtcc acatgtaggt
     1081 cgctgatgat cgccaatttt cccattttat ccctctagtc ttttcgaaca acttgcaagt
     1141 tgccttttct caagccgctt ggtcctttta attgtaaact attataagtt ggttctaaag
     1201 caagtgtgga tggatcatcc atgtaaagta ttgcactgtc tttgattttg attggtccaa
     1261 ctggtgtatc agccactaca gaataaagac ttagatctgt ttgtgtatct tttttgacaa
     1321 tgatcaatcg aaatgccgta tccaattggc aactgacttg gctttctgca aatggaccgt
     1381 caccgttttc gatatctaat aaaagttgat cgtctgcttc taatttattc ttcaatactt
     1441 cttgtgcttc ttttgtcact tctaatttca tctgtatcac ctcatgtcat agaataatca
     1501 ctttattcat cagatgcaac taatttgtct ggttctgact gttctcaatt tattggaaag
     1561 taagtcattc gtttattatg atttctctta tcacaaaaga gacaactttc ccttttcttc
     1621 gttcagtttc ttgaacggaa aaagacccaa aaagcaacct gttatttgtt taaccttatt
     1681 cctttcttaa ataacaaaaa atactataaa aaataaaaaa cctttaacaa ctattggaaa
     1741 aagaattact ttttgttcac ggattattaa caactcttcg gtagagtaaa cttatcaaat
     1801 aaaagacttc tttggaggaa tcactatgaa ggaatatata aaaaacatct attttatcga
     1861 agaaacacaa aatatcgagg gttcttatat tgaagtaaaa acactctttg tcaatgaaga
     1921 taaaaccaaa gcattggata tttataaaaa attagcttca aaaaaaacga atagttttgg
     1981 attgatatta agcgagtata aaatcaaagc agaagagagt tatttctatc aattactaaa
     2041 acggtggagt aagcttcccg ccgattttta tcgaaaaatg cagatcatca actatcaacc
     2101 gcttgctgag acacatgcct aaatagaggt tgtgaaatca gtgctttgag ctaataccga
     2161 aggtcagctg attgaacatt gtttagaaga gattgtgaca actgcgattt gctttgagta
     2221 ataagaacgc tggacagaaa atagcttctc atattttcta gtctagtggg cttattgccg
     2281 agaagcagca gatggaacat cgtttagaag agattgtgac aactgcgatt tgttttgagt
     2341 aataagaacg ctggacagaa aatagcttct catattttct agtctagtgg acttattgcc
     2401 gagaagcagc agatggaaca tcgtttaaag aagagatcgg tacaaaataa aaatatctaa
     2461 atttaagttt tatctaagtt tcgctttgta cctttgattc aaatcaaagg aggcgatctt
     2521 gatgctgaaa aaatggatca cactattttt tgccactccc cattctagaa tcaacccgta
     2581 tgatgtagat aatgaattca cgttgaaaaa agatttctca aataaataaa aaccatccgt
     2641 aaaatcatca atacgatttt acggatggtt tttgataact tctttgagga taggctcttg
     2701 ctatttaaga atgtaagaaa cattattgtt acatagtatg agagtttaat cctctgttat
     2761 ttatgctatt taaatgcttg attcacatct tcgatcatca gtttcattga ttctaaaccg
     2821 cccccgctta aataccagac atctggttca agggagatga tctgctgatt tttaccagca
     2881 ttcgtttgtg ctaccagttc attggcagaa atatcatctt tgctgtcgtc tccaccaata
     2941 gcttttgttc tgtctactac aaataagata tccggatttt tttccagcac atattcataa
     3001 gaaacacttt gaccgtgagt ggatgcttcg atttgatcat ctgcttgttc aaagccaaat
     3061 aaatcatgga tgaaaccgaa tctagaacca gatccgtagg ctgataattg cccttcattc
     3121 acaagtgtga ctagtgcttt tttgtcagta gcttctgctt tctttttcgt ctgatcgatt
     3181 gcactagata attcctctaa ttgagtttta gcttcttctt gcttaccaaa gatctcccct
     3241 aaagcagtga cattttgctg gaaagattcc catggatttt tcgcatctaa agaaaggaaa
     3301 atggtcggag caatttgact tagatctttt tgaaaatctc tttgtcggcc agaaatgatg
     3361 attagatctg gctgaagctg attaattttt tctaagtctg gttcttttat tccacctgct
     3421 gattctactt ttttatagga tgaaagatac tcaggaaggc tgcttgtagc tgctccaact
     3481 accttatctc caacaccaag agcatccatc gtatctaaag aaccattgtc gaatacgacg
     3541 actttttctg gattatttgg cacctcgatt tggtctccat tactatctgt tatcgtcaat
     3601 gtagtttcag tttgtgtgct gctttttttt gtgtctgcct gactggtgtt gttgctattt
     3661 ccacatgctg ctaaaccaaa tgatgctaga atcaagatac ctactactaa ttttttcatc
     3721 tcttctctcc taatgttagt tttagaaata aagacagaat ctgcggcctt caatctcaca
     3781 aatccgtatg tccatttcgt ataattcatc caatacatct ttttttatca tttcttgggt
     3841 cgttccttgc gtaaataact gtccatcttt cattgcaaca atttcgtctg cataacttgc
     3901 agcaaagttg atatcatgaa gaacaataac aattgttttc ccgatttctt gtactaaatt
     3961 ttttaaaatc tgcatgattt gaaccgcaaa attcatatct agattgttca aaggttcatc
     4021 cagtaaaata taatccgtgt cttgcgctag caccattgca atatacgcac gttggagttg
     4081 accgcctgaa agagtgtcaa tcgattgatc cgctatttct tctaacccaa ggtgttccag
     4141 tgattcctga acgatttcct cgtcttttgt cgtcaatttt cctttcgtat aaggaaatcg
     4201 tccaaattct actaattctt tcacggtgat ttggacattt atttcattcg attgtcgtag
     4261 gatcgataat tttttggcta attcattgga tttccaagcc tttacttcgt tatggtctaa
     4321 atagatttca cctgtatctt ttggtatcaa acgactgatc atcgacaaca ccgttgactt
     4381 tccagcacca tttggaccga taaaagcagt caacttcttt tcaggaatcg ttaactgtac
     4441 atctttaacc actgattttc catggtacag tttattgacc ttttgtaaat caatcatagg
     4501 atactcttcc tttcgcgaat caattttgtg acaaaataaa ttcctccgac aaattcaacg
     4561 acgacactca ctgttgtatt ccagccgaaa atctgctcta ctgcgagctg tcctcctgtc
     4621 agaaacagca tagctaccag aaagcttccg ataaatagct gaccatgacg ataagtagaa
     4681 aagacacgat aactgatatt tgcgacaata aaccctaaaa aagtaactgg acccacaaga
     4741 gccgttgatg tccctgtcaa aagactgaca caacataaaa tcaaaaattc aaaacgatct
     4801 gtgtctatcc cgaggtttac tgcttgttca tttccgagat ggaaaacgtc tagcacatgt
     4861 cgatatcgaa ataacaggta ccagcagccg ataatgatca aagaagcagg caaaagatac
     4921 tgactctgga tattgccaaa gcttgcaaat aatctgcctt gaagcaaatc atattcattc
     4981 ggatccatga tgacttgtaa aaaggtactg acactaccaa aaagcgtacc taaaatcatc
     5041 cctgacatca gtaataaaaa aagattcgct ctcatttttt tcaaaagaaa gaaaaataac
     5101 aatagactca gcccgaccat cagcccaacg ttcaataaga aaaaaactgt acctgtctgc
     5161 tgaagcatcg tcactcctcc gataaagaag aaaaggaccg tttggacaag gacatataac
     5221 gactcaagtc ccaatactga aggagtcaaa aactggttat gcgtaattgt ctgaaagctg
     5281 attgtggcac tgctgacagc tagcgagaca aggataaatg caaaaatttt cttactcctc
     5341 aatgcacatg caaaatgcca gtttccatat gtatggatcg tcaagtacat tcctgtaaca
     5401 aataaaacaa gaacaagcat accaataaaa atagatggct tttttttaag catatcgtcg
     5461 tccccttatc agaagataaa ggaagatagc cccaccgatg attccaacga tcacactgac
     5521 tggtatttca taaggaaaaa tcaacacgcg agcgacaaga tcgcaaaata ctaagaacaa
     5581 actacctgct aaagcagtta cgccaattgt tttgtgcact tgatctccat atattctaga
     5641 gacaagattc ggaatgatca ctccgataaa aggaatgctt cctaccatga tcaacatgac
     5701 tgcactagcc aatgatacta agccaatgcc taaaaactgc atcttctggt aatctattcc
     5761 cagatttact gcaaacgatt cgcccagacc aatgatcgta aatcggtaag caaatagcca
     5821 aaccaagagc aacaatggaa caacagcata caataactca taatttcctc gtatcacagt
     5881 cgcaaaattc ccttgaagcc atgaagtcat attttgtacc aactggaatt gataagcaaa
     5941 aaaagtagca actgaaccaa tgatattccc taacatgact ccaatcaacg gaagcatcaa
     6001 ttgatttttc tggaggaaca atcgcgtaag ggataaaaaa agcaatgttc ctgcaaaagc
     6061 aaagcaaaat gctgtcaagg atcggacaat caaggatgca ccaggtagga aaatcatagc
     6121 cactaaaata cctaaacgcg cactatccat cgtgcccgca gtcgttggtg aaacaaattt
     6181 attttgggtc aaatgttgca tgatcaaccc acaaatacta ctggtcgctc ccacaaggat
     6241 caaacaaatc gttcgtggca aacgagtcac tagcaagatt tgtaagcttt gcccatctaa
     6301 ctggagcagt tctgacaatt gtacattcga tactccgatg aacaatgaaa tactacttaa
     6361 aataactaat aatgctacta gtcgtccgta gcgcatccaa tccacctttc taattgagaa
     6421 tgattttcat tcttatttta agtgaatcgt tgaacaaaag caaccgcttt ttcatatgtt
     6481 ttaggtatta ttgggaatat gctataatcg taagaaagac aaagagaaga aggagggtat
     6541 tttttatggc acaagatgat gtaaaaagcc atttggataa gggactttat ggtacacctt
     6601 tagtgaaccc tgaagaacag cataaatatt tagggacatt cagggaacgc tgctatctta
     6661 gtatgactgt tgctgaaatg atccaaccaa aaaacaaaga aaacttttta aaagaactca
     6721 aaaaacatcc agatgcttcc atactcttga atggggctct acccctttct gtccaaaacc
     6781 aatatatcca gctagcaacg aaaaacaata gccgtttcac tgtagtcaac gattacgtca
     6841 ctgcaaatcc tgaggaattc ggattgctct taacagcaaa agaagcagta aatgaacctg
     6901 tgattgatat cgaaaaaaaa tacccagaag aaaactcttc gccagctgaa aaagaaaaac
     6961 caaaaaagaa aaaaagcttc tgggatcgtt tattttaaaa cgaaaaaaca cataaatttt
     7021 tacatgcaaa aaaagctttt gatcagattg acatcaaata ggacaatctg tcaaaagctt
     7081 tttcttttaa aatacttcag gatataacaa tttcaataca ttcatcgtca atcttctgcc
     7141 ttttcctgca aaaggataaa catgcatatg catggctgga ttgaaatttg cttcgatgat
     7201 cccatatgct tttttatcag ttgtaggatc gatttcttta tctggtataa tcaaatcgat
     7261 accactaatc ttagctccta gtgcttctac cgcagaaacg gcgatttttt tgtaagcttc
     7321 agaaaattca tcagtcatat cgatcgaatc accacctgta ctgatatttg agttctccct
     7381 tagataaaca atttgattgg cttggggaac tgattcgatc gtcaaccctt gttctttcaa
     7441 catcaattgt tctaattcac ctaattggat caattctaaa ggtgcacgat gattcgttcc
     7501 acgcaaaggg tcactatttt tttcttcaac aagttctttc actgttcgga tactgtcacc
     7561 aataacatta gcagggactc gcaacatgat ggcttggact tctccgtcga tcacaaagaa
     7621 acgatattcc gttcccggca taaattcttc tactaaaacc gaactgtctt cacgaaaagc
     7681 aatggctaaa cctgcttgat aatcttccag actagctcct tccttgaaaa tcgtgatacc
     7741 taacccgtaa ttggttgatt taggtttgat cacaaatgcc tgctcggcaa atctcggata
     7801 agcttttacc gcttcttcca tggatgaaaa ttctgcgcct cctggtaccc ggaacccagc
     7861 ttcttttaag acttttttcg ttactgtttt gttttccatg ataagtggga cgatatagct
     7921 gtccttactt gtcatgtttg catttttcac atattctaca tggtcttgga attgcagacg
     7981 caaaaattga tccgactcat ctaatacttt gacttggaca cctttttgga tggcatcgaa
     8041 cataaatatc tgagtagaaa gttccatttc gcgaaaaccg gccaactgat agggacgttc
     8101 atggctttct ttataatatt gatgtcccaa tgccacagca acttcttgct gactcgatag
     8161 ctgtgcttct tgatagagtt tcgctgctaa cgtttgttca ggatgatcca tttgagtcaa
     8221 tgcttcctct actaaataaa gagaatccaa ccaatccaat tcaaccagca tttgcttcat
     8281 tccttcaaga atttctctac cttcttgatg aaattctgtt tgatctgatg gttgttctag
     8341 tgctactttg ttatttcgag tcgtaccttc tgctacccat tggtcacagt cttctttttc
     8401 ttctgtccat aacatgtaca tcaggaataa ttgtaagaac cgaacttgct ctggattgat
     8461 acccaatctt gcataaggat tcaaatcgat attccgcaat tcaatataac gaacacctgt
     8521 tgttgctaaa tctgcgactt tctttccgcc tcggaaacga agcggtgtat aaaattcttt
     8581 ctcctccgaa agtttccctt cttcgatcat tcgctcgata tctgctaaat actgtttcat
     8641 agaagcatag gaaactttca catttggatg attcgtatag cccagcgcac tgttgcgaat
     8701 actgcgaaca ggttcttgcg gatgtgattc atctaaaaag tagttcttct cactcattgg
     8761 tgaagcaccg aataaataag tgatcatcca gcgatagcgc ataaagtttc tcgctgtttt
     8821 caaataaagt tctgttttga attcggaaaa atcttgaaat tcttcctgat ggctgaacaa
     8881 tgttcttagc aactcatcgc caaattcaaa attgaaatga ataccgctta ccatctgttt
     8941 tcgcttcccg tatttttttg ctaaataacg tcgatacaag acatcttcga aattttttaa
     9001 ttttgcaata atgatctctt cgtctttttc cggtaaggca ggtggcatac ttaatggcca
     9061 gatcatctct tctttcggta tagaacgagc agtcacatca taaacagcag cgagatactg
     9121 aaataattcg ggaatagaat cggtaacagg ggtgatcatc tctgtttgtg tttcgctaaa
     9181 atctgtttgg atataggggt gataggatcg atcgccaaag attgctggat gatcggtttt
     9241 tgctagattt cctgcaagat caacccgctg tccttcccgt tctataccaa atcgagcttg
     9301 gtcgataaaa gggcgcgcat tgacatgcaa taataattgc ttaaaattca tcattaagtg
     9361 acctccaatt catatgaatc taattataat caaaaagtct aagaaaaaga acgaaattgt
     9421 ttgagaaccg cttacaaata agtataaaaa tgaaggcatc aaaaacacag ctatgttaaa
     9481 aacaatcatt caaacaagga gctgcaactt ttgtcacagc tcctgctttt tagacatggg
     9541 ttattcttct tttagtaatc ctttgaacaa tccaacgaca aaatcatttg gatcaaacgg
     9601 ctccaagtca tcgattcctt caccaagacc gaccaatttc actggtaaat gcaattcatt
     9661 acggatagca agtacgatac cgccctttgc tgtaccatct aattttgtca aaacaagacc
     9721 ggtcacatct gttgtttctt tgaattgttt tgcttgaacc atcgcgtttt gaccagtggt
     9781 tgcatctaat acaagaagtg tttcttgggg tgcttctggt aattcccgct ggattactcg
     9841 ttttattttt tctaattcgt tcatcaagtt cactttattt tgcagtcggc ctgcagtatc
     9901 aactagtaaa acatctgcct gctctgtctt tgctctttcc aaagcatcga atacgacagc
     9961 tgctggatcg cctcctgcat tgccacgaac aacttcaaca ccagcgcgtt cgccccagac
    10021 aactaactgg tcgattgctc cagcgcggaa ggtatctgct gctgccatca atactttttt
    10081 accttcgttt ttgaactgat gggcaagttt accaatactt gttgttttcc ctacaccatt
    10141 aacaccgaca aacaaaataa ctgttagtcc gtcttcttgg atatttagtt catttacttc
    10201 attgattcct tccgcttcat agagatccac aagtttctcg atgatcgcat tttggacttc
    10261 tgccgatttt ttcgcatttc tgagtttcac ttcttgacgt aatgcttcgg tgattcgtat
    10321 cgctgtttca aaacctacat ctgcaccgat aagtgtttct tctaattctt caaaaaagtc
    10381 ttcatcgact gttcgaaaat tggcaaacaa ctcattcatc cgttcaccaa atgttttacg
    10441 cgtcttttct aaacccttgt cgtacttctc ctgtacttca acttcttctg ttttctcttc
    10501 gccagtaaaa gcacgcttga ttttatcaaa aaatcccatt tgactccccc tactctaata
    10561 caaatttttg gatcactttg gctacgccat tttcttcatt gctggctgtc acgaaatctg
    10621 ctgcttcttt gacgaaaggc acagcattct ccatcgctac gcctagtcca gcatattgga
    10681 tcatcggtaa atcattttcc tcatcaccga tcgccatgat ttcttcttgt ttcaaaccaa
    10741 gatcttttgc caataaagaa ataccataag cttttgtaac accttttggc ataaattcca
    10801 aaagcatact tcttgttttg atgatctcat acatttcatg gaattctgct ggtatttctt
    10861 tgattttagg atctaattct tcttggtcat aagcgattac tgccttatta agcacaaatt
    10921 catctgtaat atcgcttata tccgctggtt ggaattgcag cagcttattc aacacattgt
    10981 acaaggattg cttccttggg gcactaggca gctgtaaaac cacattgtca gacaaaacat
    11041 ctagtggcag atccaattgc tgagccagtt tgtataagcg ttggatatct gatacttcca
    11101 tcactttctt ttccatgatt tcaccagtat catttttttg taccaaaccg ccgttgaaag
    11161 tgatgctgta gtcaccttca tctaccagtc ctaattcttg cagataatgc gccatcgcag
    11221 ctaaggggcg tcctgtacat agaacgatct tgaccccttg ctgttttgct tgtgctaatg
    11281 cttgtttatt ctcatctgaa atcagctttt gttcatttaa taatgtaccg tccaaatcga
    11341 ttgctactaa tttgatcatt ttcgttccta cactttcgtt tcgttgattt tcgcctcttc
    11401 attgatatct tccatccgta cagaaacgat ttttgataca ccggattcct gcatcgtcac
    11461 tccgtacagt acatctgctg cgaccatcgt tcctttgcgg tgtgtgacta cgataaattg
    11521 tgtgtcatct tggaaatcgc tcaagtaacg accaaaacgt ttcacgttgg cttcatctaa
    11581 cgctgcttct acctcatcta agatacaaaa cggaacagga cagacacgga taatggaaaa
    11641 gagcaacgcg atggctgtaa gtgctcgttc tcctccagaa agcaaactta gactttgcag
    11701 ttttttgcca ggaggttgta cttcaatctc aatacctgtt ttcagcaaat cagttggttc
    11761 cgtcagtact aattcagctt taccgccgcc aaacatattt ggaaagacaa ttttgaattc
    11821 ttgacggata gcttcaaata cttctttgaa tcttgtccgc acttcatcat ccatctcatc
    11881 catcgtttca aacaattggt ttttcgctgc taacagatca tctcgctgtg ttgctaagaa
    11941 tgtatgtctt tcactgactt gttcgtattg ttcgattgaa tttaagttta ctggtcccag
    12001 tttttcaatc tgttctttta aggatgaaac ctttgttcta ctgtcttcga tatcggttgt
    12061 ttcttgatag tcttgggaag ctttctcaaa tgtcagctga tattctgact gcagataggt
    12121 caaatgatta tctaaaatca tttcagctcg gtctttttgg acttctaatt tggattgttc
    12181 cgacagtcgt gctttttgct cacgattacg ctctgccaag actgcttcaa gcttatcgat
    12241 ctccttttgc cttctatctc gctgctcttt ggcttttgcc aactcggctt ttaatgtttc
    12301 tcgctgagca gataattcgt taatttgttt ttccaaactt tcttctgtga cttcatgatc
    12361 agaaaagtct gctgtcaatg tcgccaactg cttttctatc gcttcttgac gttcggttgc
    12421 ctctgctttt tggactcggg caccgcggag ctggatctgc agatgattaa actgctcttt
    12481 caagacagca agatctgctt gttcttgtgc tttttgactt tgtacttgtg ctcttctggc
    12541 ttccatctgg tcgctttctt gacttaacga tttgatttct tcgtcgattt tttgtcgttg
    12601 cgattcaaga tctgtttgtt tgtctttcaa ttcgttcgtt tgcttttcgt aatcttcgat
    12661 aaactgctgc agttcacgtg tctcaaaagt tgagatttgc ttttcttttt caaaacgttc
    12721 aagttcattt atgatgtttt gtagctgatt tgtcgcttct tgttcttcaa aacgtaattg
    12781 ttcgcctcgt gtacgcaaaa cttcttgttc ttcggctaaa cgagctgttt cttgttgcag
    12841 ctcctgaact tttttttctt gtgcttggag ttgtttatct gcttcttcaa attcactagt
    12901 caactgtttc aattcttgat tttgtacaaa caaactgcct tggtttcctc gtttattcgc
    12961 tccacctgtc atggatcctc cggcattcat cacatcacct tctaaggaaa ccactcgata
    13021 ttgatagcgg atcgtttgcg cgattgcatt ggcactagtt aagtctttgg ctaaaaggat
    13081 cgttcctagc aggttttgaa ccacagtttg gatttgatcg ggataactga cttgttcact
    13141 ggcaatccct ataaaccctt ctacagctgc tgcttgtgtc aatatatgag ccggtaattg
    13201 tcttggtttg attgtcgtca gtggaaggaa tgttgctctt cctcctcttt gctgtttcaa
    13261 atacgtgatg gcttgccgag catctttttc attttcaacg atgacatgct gggctgcccc
    13321 acctaaagct gcttcgatag ctaacgtgaa atctgcagga acatcgatca gttcagccac
    13381 tgctccgaca atacccgaaa gctgctgttt atgctgcaag accaatcgaa ccccttggta
    13441 aaaaccaaaa tagttttctt ggatttcttg aaggcttttc ttacgagcac gtagctgctg
    13501 gacttgattc attaattgat acattttagg ctgttctttt gctaatgctt cttggaactt
    13561 tttcccgttt tgttggatca tttctaactt ctcttttgtt tcagttaaag atgattttag
    13621 ctgcgcctgc ttttgtgtca attcttcttt ttgtaagatc aatcggtcta ctgatgcttc
    13681 tacttccgat tgcttggcta acgtctgctt gctttttgct gtttcttgta tatattgacg
    13741 ttctagatac ttcagttcgt ttcctacagc tgcttgttct tgcatcaaat caacatattg
    13801 atctcttaat tctgccaata attctttcgt tgatttttgg tatttttcta attcttgctg
    13861 tgtttttatg agatttgctt cagctttttg gatctctgtt tctttttctg ctgctgcttt
    13921 catcaagctt tcttgaagct tttcaaaatg tttaaccttt ttttgtgctt ctgctaatga
    13981 agtttgatat tcttgcgagc ttttttgtgt atgctttgtc cgctcctgca aaacatcttt
    14041 ttgtccttct gtctgcttca gtttttccga aagatctaat aatacttgct gatttttttc
    14101 gatcagacga tcggcttgcg cattttcttt acgctgcttc gccaaaatgg attcttgttc
    14161 ttggatcgac tcagatagtt tgcctaattc tagattgaat ttcgcaagct gtgcttgttt
    14221 gttgtcccaa tctttttttg ctgtcttgat ttcagcaacc atcaaagaga catcggtttg
    14281 agtcagtgtt tcttttagcc gtaaaaactc ctttgctgcc tcactctggg cggccaaagg
    14341 agttaactgt tcttccaatt cgtggatgat gtcttggacg cggctgaggt tgtcttctgt
    14401 ttcaaaaagc ttctgttctg ctttcttttt ccgctgcttg tatttcaaga caccagctgc
    14461 ttcttcaaag atcccacgac ggtcttccgg tttactactg aaaatcgctt ctacttttcc
    14521 ttgagaaatg attgagaacg attcttttcc taatccagaa tctaagaaca attcttggat
    14581 atcttttaat ctgcagcttt gtttattgat gaaaaactcg ctttccccag tccgtcgata
    14641 acggcgggtt acactgatct cattgtactc caatggcaga taatgatcac tattgtctaa
    14701 aatcactgtg acttctgcaa tattcagctg cttacgggta tcggaaccgg caaagatgat
    14761 atccggcatt tttccgcctc gcagactttt agcagattgt tctcctaata cccagcggat
    14821 agcctctgta atattgcttt ttccgctgcc atttggtcca accactgctg tgacgctatt
    14881 ttcaaaatca atgactgtct tgtctgcaaa tgatttaaaa ccggctattt caattcgttt
    14941 caaatacaaa gctttccctc acttactttt acagtgctgc caatgcattt tccgccgcag
    15001 cttgttctgc taattttttc gatttccctt gtccagtgcc aatcaattga tcatctacat
    15061 aaacttcgat ccaaaatact cgttcatgag ctggaccttc ttcattgatc aatcggtatt
    15121 cgattgatac atcacctgat ttttgcaaga cttcttgtag ttttgtttta tgatccatct
    15181 catgtgaaaa agcacctgct ttgactttag gaaaaatcac tttttcgatg aatgcatggg
    15241 tcgtgtcgaa tccctggtca agatataacg cgcctaaaaa agcttcgaat aggtcgcaaa
    15301 gcaatgctgg tcgcgtacgt cctcctgaat tctcttctcc cttgcccaac aaaacatatt
    15361 gatcaaagtg acattctttg gcaaatttcg acaagctgtc ttcgcgaacg atcgcagctc
    15421 gtgtttttgt caattttcct tctggcaaat gaggatataa acggaataaa tactgagaaa
    15481 ccatcagttc taaaacggca tctcctaaaa attcaagtcg ctcattatcc gataattgca
    15541 gattgcgatg ctcattcaca taggatgaat gggtaaaagc ttgttcaagc aaatttagat
    15601 cgtgaaaaac gatgttatag ttttctttta gttcttttgt cagctggttg tccattttcc
    15661 acattccttt tatggatatt ttttcgatac ttccttatta tactggtttt ttgtcaaatt
    15721 tgaaatggtt cggtcaaaag gtaagtaaag ctttaataat cctagtgatt gacgagaagt
    15781 tgtgaaaaat tcgtttagtt tcgagtatga agaaaaaatt ttgtgaaaat ggctttccat
    15841 atttttgcat aaatttgact taataccgag aaactggact ttgaacaccg tttattggag
    15901 aagtcatgaa atcagcgcct tgacctgaat attagagaaa taaacagaaa aatatcaaaa
    15961 aaccgaaaaa ggacatcgcc ctttttcggt catttttagt gattatgaac gtgaatctgc
    16021 tgtaacagaa acatttgccc atgggtttgg tacatcataa gaccaatccc agtctttgac
    16081 acggtcgttg actggcaata cttcattacg gaacaacgtt ggaatcgcaa atgcttcttc
    16141 aaatgcatat tgttgccatt tttgatatgc ttctttttgg tactcttcat caaatgcttt
    16201 gcttgaactg atatcgttga tcaattttgt attttcttct gattcataac gagtgtagtt
    16261 gaacgcagca tttggtcccc atagtccact tggatttgga tcagttcctg tattccaagc
    16321 agcttggtaa acatcaattt caggatcatc gttttctaat ttatcataga aagaattgaa
    16381 ttcgatcaga cgacctgttg tgtattggac atccaaacca atttctttcc attgttggat
    16441 atagtaatct gccaatggtt gcgctgtttc cccaccggac atcgaagcaa agttgattgt
    16501 tagtttattt ccatctttgt cttctcttaa accatcgtca tccacatctt tgtagccagc
    16561 atcatctaat agttttttcg ctttgtctag atcttgtgtg aatcctttca tctcatcatc
    16621 atggtatgac tggaatactg gaggaatcaa tgaagtcgca ttgcttctca aacctgcata
    16681 gaaacgttca cctactgcat tattatctaa tgcatagccc attgcttgac gtaatgattt
    16741 atcagccatt tttgaatctg gattgtattc cacactcttt gtatctgcat tccatttacc
    16801 taacttgaat cctagataag tataagaagt ttgttcacgg cctaagtttg tatagccagc
    16861 aatttcttta taggtatcgt aagtatctgt tggcatttgg aaaatcatat catataattt
    16921 agagttcaat gcttcagttg cacttgccat cggtacagat ttcatcgtga tctttttcaa
    16981 tttaggcgta ccattccagt agtgctcgtt tggcaagtat tctactgatt ctcctgcaac
    17041 gatattactc atgtaataag ggccaaaagt cactggattt ttacgtactt gatcgcttga
    17101 ttccatatct ttgattgcta caccttctaa cgtatgtttt ggtgctgcat atgaccagat
    17161 accaccaccc gattgaagca tgcttgggtt tacttcttta tattcgattt caactgtttt
    17221 gtcgtctatt tttttgattc cggagattgt gtcggcactt ccatcatgat attcttccat
    17281 acctacgatg ttacggaaag tgtcgtcata acggatacca gtgtaatctt tattcccaat
    17341 gatttcgtaa gggaaaatca catcgtccgc cgttacatct tcaccatctg accatttgac
    17401 gttgtctttt actttgattg tcgctttttt cgtatcttca tctagatcaa gcgttgcagc
    17461 tccgtcgtct gtgattctga aatcgtcatc gtttgcaaat agcggttcat gagaaggagc
    17521 cataaaagct gcatcatatg aatcttggta aaactcccac tggaacaatc ctttaaactg
    17581 actgtccgtt gcgaccgcag cttctaattc gccgtcttcg attgcatcat cattattttt
    17641 tgttgcaatt ggaaaagtta attcctcttt gttattgccg ttgcctccag agttgtttcc
    17701 agaggtatct gtacctccgc ccccgcatgc tgcaagaacg acagcagaca tcagcgctgc
    17761 aaatccaaaa atcgttttct tcattcctat ttcccccttc gcttctatac tagcctaacc
    17821 gttggcgcgc atctgcagaa cgcttgaatg cttgtccaac ataattgata ctcaacatca
    17881 aaactaaaat aaggattgat gcgggtaacc agatccattg ccgattcact aagacttctc
    17941 cgttacttgc ataaccgatc aatgtaccta aactaggtac atttgccggc aagccgaaac
    18001 ctaagaaagt caaagttgtc tcgataccga tgtttgccgc aaagttcatc gtcaaattag
    18061 taatgattaa tgaacttaga ttcggcatga tttcgcggaa catgatttta aaatcattgg
    18121 ttcccattgt ttttgaggca ctgacataat ctcttcttgc ttcagataat gtcttgcttc
    18181 ggaataatcg ggctttagct acccaataaa atgcactcat gatgaatata aaggaccaga
    18241 cattgtatct ggaaataatc gatacgaaga cgataatgat cagcatgatc ggtagaatca
    18301 tgataaaatc aacgatccgc atcaagatgt tgtcaatgat ccctccgtag tagccagaga
    18361 tgatccctaa tccaaccccg atgatcgaag taaggatcgt gatcgcaaat ccgatcgtaa
    18421 cagagtttct agcacctatg atcaattgtc ctaatacatc acgtccgcct tcatctgctc
    18481 ccagaatgaa cttttgtcct tgtgtcgaaa ctgttcctgg tgcagcgtat ttgtcgaaga
    18541 tactaacata catcactttg tcttgatcgg tcagcatcgc accgataaag atcactaata
    18601 ataaaaccac tagcaaacct aatgaaaaaa tagcaatttt atctttttta aattctcgag
    18661 caatcattcg tatccccata ggaggaatac tttcttgaac tgctgtattg tttttttcac
    18721 tcatcgatcc attctccttt ctgattggtt ttattgaatc cgaatacgcg ggtcaacgat
    18781 actcatgatg atatctgaaa gtaatgtccc cactagcgtc gcgattccta aaatcaatac
    18841 taaggatgtg atcacggtat agtcccgttg gccgatggaa tcaataaata gttttccgac
    18901 tcccggataa ccaaaaatct tttcgataac tacagaacca gcaatcaacg aagtgatttc
    18961 ataccccatt tgagaagcca tcggcaacgc tgcatttcgg aaaatatgtc gactgaatac
    19021 tttattcgtc ggtacccctt tggaacgggc tgttctgaca aaatcgagcg atttcgaatc
    19081 gattacttca cttcgcagat attggattgt gacggcagtc gccaaaaatg cttgggtaat
    19141 tgatggtaac agaatatatt tcaaacggtc tatccattga gtgataaatc caccttgcaa
    19201 acctagtgtt tgtgatccac ttgttgggaa ccagcctaaa cgataaccaa aaacaaatag
    19261 catgatcaag ccgaagataa ataaaggaac agcaaaacta aagaagttgt agattacgac
    19321 aatcttatcc aagattgaat tttgataccg gccagcaagc atccccagag gcacagcaat
    19381 caaataagta aggacaactg tcaagatcga tagatataaa gtgttgatga tccgttcgcc
    19441 gatcaagtca gcaacagagc gtttgtataa gaaactttga ccaaaatcgc cttgcaaagc
    19501 atttccgatc caacggaagt actggacata ccatgggtca ttcaagccag ctgcttgtcg
    19561 catctgttcg atcactgccg gatcctgatt tggattgatc aaaccggtaa atggatcccc
    19621 cggcatcgct tgggctaata agaagatcag aacacttaaa ataataactt gcgggatcat
    19681 caataaaaca cgtcgcagaa tcgttttcca cattagcttg cacctccatc tttcaacgcc
    19741 actttgtgtg tagttgtgat cgtacgcaaa tcgtagactc gaccagtcgc atcatagtat
    19801 tctttttggt tttggatata ctcttgttct actcttcgtc gattttcttt gtggctttcc
    19861 ctattttcta catcgatttg cggaatagct gaaagcagac gtttcgtata gatatgtcgc
    19921 gggtcattat aaatgtcttc tcttgtaccg atttctacaa atcgaccttt gtacataatt
    19981 gcgatattgt cgcacatgtg ttttactaca cctaaatcat gtgaaatgaa taaatagctg
    20041 aggccgtatt cttgctggat tcgtttcatg aaattcaata cttgcgcttg aacagataga
    20101 tccagagcag atacaggttc atcggctacg atcagttttg gattggtagc aacagcacgg
    20161 gctactccta gacgttgacg ttgtccgcct gaaaattcat gaggatattt atatagcgca
    20221 tcttccggca tcccaacgat atctagcagt ccttttacct ttttcttttc ttcttgatcg
    20281 cttaaccgtt caaaatttcg gatgggttca gcaatgatat ccaacactcg ctttttagga
    20341 ttcaaactgg acattgaatc ttggaagatc atctgcacat ccttgttata acccattttt
    20401 ttgcggttgc ttcgcttcgt tacatctttt ccttcataca tgatttgtcc ggctgttacc
    20461 ttttctaatc ccacaacggc ttttcccgta gtagattttc cagagccaga ctctcccact
    20521 aaaccatatg tctttccttt ttcgataata aaatccacac catctacagc atatacatga
    20581 tccgttactc gattgaagaa tccactgcga atagggtaat gaactttcag gtctttgatt
    20641 gtgattaatt ctgtcatcta cacctctcct ttttcgtctc taaagtggaa atgcttatag
    20701 caggagcaac gtaccaagtg gttcggtgcg acttcatgaa ggataggctc ttcttcatga
    20761 tcgcttgctg gtatccatgg aatacgtggt gcaaaccgac aacctttacg aggcatgttt
    20821 ttcagagaag gaacgacacc ttcgatcaca tggagttctg catcatgatt tccttcttgt
    20881 ggtatagagt tcagtaaaga gcgtgtataa ggatgcttag gatgatcaaa cagttctttt
    20941 acacctgcta cttcaacgaa ctgtccgcca tacatcactg ccactcgatc agccatttct
    21001 gctacgactc ctaagtcatg agtgatcaag atgatccctg tttttgtttc ttcctgaagg
    21061 tctttcagta aatcaagaat ctgtgcttgg atggttacat ctagggctgt cgtcggttca
    21121 tctgcaatga cgattggagg tttgcaggcg attgcgattg cgatgatgac acgttgtctc
    21181 ataccgcctg atagttcatg gggatattgg cgtccaactc tcgcaggatt aggaatccct
    21241 acttgttcta gcaattctag tgcacgagct tgacgctgtt ctgcattcat ctttgtgtga
    21301 taggaaagtc cttctttgat ctggtcttcg attctcatga gaggatttaa tgcagacaac
    21361 ggatcttgga aaatcattcc gatatcattt ccgcggattt tattatagag cgtttcgttc
    21421 aggtcgatta aattcagatc gttatataaa atctcacctg taatttttgt gttattagga
    21481 tcaagtaatc ccataatcgt agtagccaag gtacttttcc cgcacccaga ttccccaacg
    21541 attgctaaaa tctcgttttt gtctaaagta aatgaaacgt catctactgc atcgtaatag
    21601 tcatctttga gtcggaatcc tgtatgtagg ttctgaactt ctaataattg gttatcggac
    21661 aatcgaatac ccctctctgc ttcctaaatg gaagtttttc ttaataaaaa aggacattac
    21721 acagaataaa gcgttttata acaaaggtta ttcttatctt tgtgtaatga attattaata
    21781 attatataga aaatcgcttt actttgcaac ataatttctg tttatcgcca gaattatcag
    21841 aaaaatgact tttacgtgtc aaaaagatta aaaaaagaag gataaaaagc tgtatgcagc
    21901 tttttatcct tctttgcttt tatttgaaat tatttaagat ggctgctgat ataatctact
    21961 gcgccaccga ctgtttcaat ttgttcagcg tcttcatctg aaatttccgt accgaattca
    22021 tcttcaagct ctaaaacgaa ttccataata ctaattgaat cagcttttaa atcatctttg
    22081 atgttcaatt catccgtcac tttatcggta tctatctcaa aatgatttga aataattttc
    22141 gctactttgt taaatacttc ttcgcgagtc aactttttca cctccaagaa tcatacactt
    22201 gtcaattacc tacaccatgg ctaattttac tggcattttt caggaatgtc cacctttttt
    22261 tcaatggaac ggattgcttc agatatccaa atactattct tcgctttttt caaattgttc
    22321 gactaattgt ccaactacat ctgtctcaag cattgtgtgg atttggcgga tcgtatagcg
    22381 aacagcatcg gggcctgttg ctccatgagt tttaatgact ggtgctttca agccaaacaa
    22441 aacggcaccg ccatgtttgg aataatccat ttctgctttc aaagaacgta agccgtcttt
    22501 taaaagcata gcgcccattt ttcctttgat cccttcattt agaatagcag attttaatag
    22561 attcatcata ttcattgctg ttccttcaat agatttcaaa acagcgttgc ctgtaaatcc
    22621 atctgtgacc actacatcag ccacaccatt gagtagctca cgagcttcta cgtttccaat
    22681 aaaattcaag cttgtttcat tctgaaggag ttcataggct tttttcgtca cttcactacc
    22741 ttttgttgct tctgttccgt tgtttaagag tgcgacacga ggacgtccga tacctcggac
    22801 tttacgtgca taaaaagaac ctaaaatacc gtattgcaac aaatgttctg gtttgttttc
    22861 tgcgttagcg ccaagatcca gcatatcgaa accggcacct tctttcccta ctactggcaa
    22921 ggtagacatc aagccagggc gttcgatatt tttgatacgt cctacgatga acaaccctgc
    22981 tgctagtagc gcgcctgtat tccctgctga aaatacagca tctgcttcac cgtttttcac
    23041 tgcttgtgca gcaaggacca ttgatgcagt cttttttcgt cgaattgctt ttactggctc
    23101 atcatcacta ttgatttttt cgtctgtatg gataatcgtg atgttttttt catttgttaa
    23161 gtatttcttg atttctgctt cttttccgta aagtaagaat tctacttctg gaaaatcttg
    23221 ctgcgctaaa gccacgcctt ctacaattgc ttgaggtgcg tgatctccgc ccattgcatc
    23281 tacggcaatt ttcattgttt ccactccttt ttccgtcatc agtataaagt atagcacagt
    23341 ctgctaatcg aaataacgat tatcctgatt ttctatttgg aggctttgcg ctaaaggctg
    23401 atactcagga agcatctgcc aatcctttat ctgccaaagg gctgcagcct cttctctggc
    23461 tacctccaaa acatttccat ccgtcaccaa gtcagcaatc gcaaattgag gcagaccaga
    23521 ttgacggaag ccaaatactt caccaggacc acgtaattcc agatcttttt cgcttagtac
    23581 aaaaccattg gtggtctctg tcatgatttt catccgttcc acacctagtt catttttagg
    23641 attagcaact aagatacagt aggaagcatc acttccgcgt ccaacacgtc cacgaagttg
    23701 atgcagctgt gccaatccaa atcgatccgc atccatgatc agcatgacgg tcgcgttcgg
    23761 cacgtttacc cctacttcaa ttacagtagt cgaaactaat atctgcatct ggttgtcttt
    23821 gaaggcttcc atgattactt ctttttcctg attcttcatt ttaccatgta acaagccaac
    23881 ttcatatttt ggtgcaaata gttcacgaag tttttcatag atttccactg cattttttac
    23941 atctaatgct tctgatcctt caatcagcgg acagatgaca tacatttgat gtcctcttgc
    24001 cagttctttt ttcgtccaat ccagtacact gtccaattga ggtgttctta cccagcgtgt
    24061 ttcgatcggg atacgtccag ctggaagttc atcaatgata gatacatcca tctcaccata
    24121 tgctgtaata gctaatgttc taggaatcgg agtcgctgtc ataaataaga catctggtcg
    24181 ccagcctttt tcacgaagta ctttccgctg gttcacgcca aaacggtgct gctcatccgt
    24241 aataactaga cctaaattct gaaattcgac tccatcttgg atcaatgcat gtgtgccgat
    24301 cacgatgtta atttcccctt gctctagttt ttcaaggagc tcacgacgtt cttttgcttt
    24361 cgttgacccc gtaagcaatg cgatggttac ttcattggga tcaaacagct gttgcaagct
    24421 ttccatatgt tgttgtgcca agatttctgt aggcaccatc aacgcacctt ggaaacctgc
    24481 tgtaacggta gcgtataacg caatcgcggc aaccactgtt tttccgctac ctacatctcc
    24541 ctgcaaaagt cgctgcatat gctgcggact gcgaagatcc cgacagattt cattcgttac
    24601 acgtttttgt gcatttgtca gttcaaaagg tagcttttga gtaaattctt tcaatttttg
    24661 gacatcataa aggatctcca aaccattcgt ttctgctttt tctgcttttt tcaatccttg
    24721 aagacgcatt tgaaataaaa agaactcttc aaaaacgaca cgtcgtttgg cttgatggtt
    24781 ttcttcgtgg tcttttggaa aatgcatact tcttacagca gaagctcggt caagcagtcg
    24841 atatttctcc acaaggttgt ttggcagatt ttcttcaatc aatgtgccgt attcttgaaa
    24901 cccttttttg attagatcaa ctaatgtcgt ttgacgaatt gatttgttga cgtgatatat
    24961 cggcgaaaag tcatccattg tttgagaacc taaaatcttc atcccgttca atgctttacg
    25021 ctttgcatcc catttaccgt atacggcgat ttcctccgat aaaatgactt tttcttttag
    25081 ataaggttga ttaaaaaaag aaacgttgaa cacatcatgt tcttgcatca tacgaaattg
    25141 caatcggctt tttttataac caaatcgatt catcacagga ggcgagacta cgattccttt
    25201 caaagtaacc ttttcttgat cttggatctc gtttagattt ctttcctgaa tatcttcgta
    25261 gcggaaagga taataagtca gtaagtcttc aatcgtttgg atgcccaagc tggcaagact
    25321 agctgctctt ttttctccga ctcctgccag catagtgatt ggatcatcta aagaacgcat
    25381 aaatttttcc tccctccctt aacaaaaaaa ggacttggtc agcgatcata cctgatcact
    25441 aaccgaaggc cttttatgaa tgaatactgt gttagaaaga tattctgaca cagaaattat
    25501 tcagcagaaa atagataagg atagacaggc tgatctcctt ggtggatctc gatttccaaa
    25561 tcaggatgtt tttctgttag tttctcagca aattcatttg cttgttcaac tgttccttca
    25621 tcacctgcca agatcgttac gatctctgtg tcctcattga tcatttgttc tagtgtactt
    25681 aacgctgttg ctgtgatatc tgggttagag atcaggattt ttccatcgat catgcccaaa
    25741 aagtctcctt ctttgatctc tacaccgtca attgaggtat ctctgatcgc gtgagtgact
    25801 tgaccgctca caacgctaga caacatttct gtcatcgttt ctttgttttc ttctaaagaa
    25861 gcttgatcat tgaatgaaag catagctgtc aatccttggg aaatcgttct tgaaggaaca
    25921 actgctactg ggatatctgc tacttctgct gcttgatcag ctgccatgaa gatgttctta
    25981 ttgtttggca agacaatcac tttatctgcg tttacttctt gaatcgcttt aacaatgtct
    26041 tccgtacttg gattcattgt ttgcccacca ctgataacat aattagcgcc taaactttca
    26101 aacagctctt ggacgccttt accagcagcg atcgctacga taccatacgg gatacgtggt
    26161 gtttcttttt tcgcaaactc gttcacttct tcatcgtgtt ccaaaatcgt ctcatgctgc
    26221 aagcgcatgt tgtcgacttt gattttgact aatgaaccga atttttgtcc ataattcaag
    26281 acttctcctg ggttttctgt atgcacgtgg actttgatga tctcgtcatc attgaccaca
    26341 agtaaagaat ctcctatacc atctaaatag ttacggaatt cttcgtaatc gaatgtacta
    26401 tcgactgtag gtccttctcc gatttttacc atgatttctg tacagtaacc aaaatggata
    26461 tcttctgtcg ccatttgacc ttggacactg cgatgatgtt ctgcattgac catatcatcc
    26521 atttctgctg gagaaggttc atatgtttca tccgcttgga attctccatt caatgcactt
    26581 aaaaagccct cataaacaaa cagcagtcct tgccctccac tgtctacgac accgacttct
    26641 tttaggactg gaagcaagtc tggagtttta gctaaagcac gctttgcccc ttttacaaca
    26701 gctgtcatta cttcaacgac atcatcagta ctttttgctt tcttttctcc agctttcgct
    26761 gcttctctag ctactgtcaa aatcgttcct tctactggtt tcattactgc tttatacgca
    26821 gtttcaactc catgagtgaa ggctgcagca agatcggaag catttaatga gacaacatct
    26881 ggaatttgct tagaaaatcc acgaaacaat tgagacaaaa taacgccgga atttccgcgc
    26941 gcacccatca gcaatccttt tgaaagcatt gctgctaatt ctcctacttt ttctgaggtt
    27001 gaatctacta ctgccttagc tccactagtc atagatagat tcatatttgt tcccgtatcg
    27061 ccatctggca cagggaatac atttaatgaa ttgacgtatt ctgcatttac ttgcagacga
    27121 ttcgcacctg cctggaccat ttcctggaac tggcttgcac ttatttctgt tacactcacc
    27181 tagataatcc tccttcagat cgactgctga gttaccagcc agtctcctgc ctagtctggc
    27241 agtacacgaa caccttgtac aaagacatta actgaattgg cagttacgcc taataatgcc
    27301 tctagattgt atttcacttt ttcttggacg ttgcgggaaa cttcagagat tttcgtacca
    27361 tagctgataa tcgtataaac atctacagca ataccattat cttcttggcg gacaactact
    27421 cctcttgagt aattttcttt acgcaaaatc tcagtaatat tatctttaat ttgatttttg
    27481 ctggccattc ctacaacacc gaacacatct gtcgcggcgc caccaacaac tgttgcaatt
    27541 acatcattgg taatttcaat ggttccagct gacgtttgaa tttttacagc catgaatgag
    27601 cctccttaac gggtaaaaaa ctcccatttt tcttctatca tattttatca tagcgcaact
    27661 tgaaataaaa ggcagttacc caaagtactc aaaaaccttg caaaatttca ggtataacct
    27721 tgtttaattt acaattattc ttctcaattg tcaattgtat ctatgaaaat gtacttgcaa
    27781 atttccattc tatatgatat gttagtctgg tatgagctga attattctat tgctccaaat
    27841 caaggcaaag gaggaaatta ccatggcaaa agtttgttac tttacaggtc gcaagacaaa
    27901 aagtggaaac aaccgttcac acgctatgaa ctcaacaaaa cgtactgtta aacctaactt
    27961 acaaaaagtt cgcgttatgg tcgacggaaa acctaaaaaa gtttgggtgt caactcgtgc
    28021 tttgaaatca ggaaaagttg aacgcgttta attgaatgat acttggaccg ttcagatagt
    28081 gaacggtttt t
//
LOCUS       DAYYCW010000025        27514 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000025 DAYYCW010000000
VERSION     DAYYCW010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.025"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..999)
                     /locus_tag="ACHAYA_001030"
     CDS             complement(55..999)
                     /locus_tag="ACHAYA_001030"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8604849.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1295..2239)
                     /locus_tag="ACHAYA_001031"
     CDS             complement(1295..2239)
                     /locus_tag="ACHAYA_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8604850.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLKIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2460..3674
                     /locus_tag="ACHAYA_001032"
     CDS             2460..3674
                     /locus_tag="ACHAYA_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8604851.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3696..5711
                     /locus_tag="ACHAYA_001033"
     CDS             3696..5711
                     /locus_tag="ACHAYA_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM8604852.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5794..7167)
                     /locus_tag="ACHAYA_001034"
     CDS             complement(5794..7167)
                     /locus_tag="ACHAYA_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8604853.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7193..7477)
                     /locus_tag="ACHAYA_001035"
     CDS             complement(7193..7477)
                     /locus_tag="ACHAYA_001035"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8604854.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7479..9494)
                     /locus_tag="ACHAYA_001036"
     CDS             complement(7479..9494)
                     /locus_tag="ACHAYA_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8604855.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9673..9978)
                     /locus_tag="ACHAYA_001037"
     CDS             complement(9673..9978)
                     /locus_tag="ACHAYA_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM8604856.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10067..11098)
                     /locus_tag="ACHAYA_001038"
     CDS             complement(10067..11098)
                     /locus_tag="ACHAYA_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8604857.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11305..12180
                     /locus_tag="ACHAYA_001039"
     CDS             11305..12180
                     /locus_tag="ACHAYA_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8604858.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12332..13174)
                     /locus_tag="ACHAYA_001040"
     CDS             complement(12332..13174)
                     /locus_tag="ACHAYA_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8604859.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13266..15143)
                     /locus_tag="ACHAYA_001041"
     CDS             complement(13266..15143)
                     /locus_tag="ACHAYA_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8604860.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15140..16561)
                     /locus_tag="ACHAYA_001042"
     CDS             complement(15140..16561)
                     /locus_tag="ACHAYA_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8604861.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16564..19953)
                     /locus_tag="ACHAYA_001043"
     CDS             complement(16564..19953)
                     /locus_tag="ACHAYA_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8604862.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20210..21601
                     /locus_tag="ACHAYA_001044"
     CDS             20210..21601
                     /locus_tag="ACHAYA_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8604863.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21674..22321)
                     /locus_tag="ACHAYA_001045"
     CDS             complement(21674..22321)
                     /locus_tag="ACHAYA_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8604864.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22329..23015)
                     /locus_tag="ACHAYA_001046"
     CDS             complement(22329..23015)
                     /locus_tag="ACHAYA_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8604865.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23021..24094)
                     /locus_tag="ACHAYA_001047"
     CDS             complement(23021..24094)
                     /locus_tag="ACHAYA_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8604866.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24316..24990
                     /locus_tag="ACHAYA_001048"
     CDS             24316..24990
                     /locus_tag="ACHAYA_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8604867.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            24995..26335
                     /locus_tag="ACHAYA_001049"
     CDS             24995..26335
                     /locus_tag="ACHAYA_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8604868.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26484..27470)
                     /locus_tag="ACHAYA_001050"
     CDS             complement(26484..27470)
                     /locus_tag="ACHAYA_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM8604869.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8006 a         5212 c         5008 g         9288 t
ORIGIN      
        1 agctatttca gctgttaagc agttttagga tttttagttc atattcattg tttcttataa
       61 tacagtgttc aatacttctc ttaaagcact tgttgatttg ctgaacattg ctttttcttc
      121 ttcgttgatt gataattcaa caacttcacg aacaccattt tcgtcaacga ttgatggcac
      181 acctgtaaag atatcttttt cgtcgtattc accagttaaa taagcagata caggcaatac
      241 agcttgttcg ttattcaaga tagcttttac gatacgtgca gtactcatac caataccata
      301 gtaagttgct tttttacgat caatgatctc ataagcagca ttacggactt tatcagcaat
      361 cacatctaat tcatcttggg caatacgatg gtctttttct acaatttcaa acactggttt
      421 accaccgaca gttgtatgtg accatgcagc aacttctgaa tcaccatgtt cacctaagat
      481 atagccgtgg acgctgcgtg ggtcaacttt taatttcaat gcgatttctt tacggaaacg
      541 agttgtatcc aatgttgttc cagtaccgat aacacgtgat gttggcagac cagattcgtt
      601 ccaagcgata tatgtcaaaa tatctactgg gtttgaagca acaacgataa taccgtcgaa
      661 tcctgatccc atgatttctt ttacgatttg gcgcatgatt gacgcatttg ttttaactaa
      721 atctaaacga gattgaccag gtttttggtt gataccagct gtgataacaa cgatatcagc
      781 atctttacat tcttcatagc cacctgacca tacagccaca ttttcatcgc cccaagccat
      841 accatctaaa aggtccaatg cttccccttc tgctttttct tgattcacat cgatcaatac
      901 taattcatta gagattcctt ggttgatcat tgcataagcg atacttgttc caacaaaacc
      961 tgtaccgacg attactactt tacgacttgt ttttttcatg aataaacagc tccttttcat
     1021 aactatttgt tactttcatg aaaagtatag tcttgtttat tcattttatc aacatgttaa
     1081 ttataatagt tgttattata atttttgatg gaaacgaatt cctgaatacg cataacagat
     1141 aaatgacaaa tgtttacacc tatatcccct gataatacaa ccaaaagttt agaactgaaa
     1201 ataaatccaa aaaaattcgc tgcaacgata taataagaca gcaatcgcac ctaaaacaat
     1261 tttccttcat atgctttagt gaaagatttt cttcttaatg tttatttctc cattcattgg
     1321 gagtacatgt tgaatacttc ttgaataatt tgaataaaga agtcgtttgt ttatatccga
     1381 tacgattagc gatttcttcc aacgaaaaac tcgtttcttg aatcagttcc aacgcaaagt
     1441 cgaatttttt ctttagaacc agttcttgaa aggaagcatc aaaatacatt ttcaatttat
     1501 tactaagata gaccggattg tagccaaaat gagctgctgt ttcttttaac gtacaagaag
     1561 taaaatgctt atcgatataa tctaatactc taagcatctc attttctgcg catccatctg
     1621 ctgatgaaaa gctttgttgt tctaataaat gagtcaattc gatcagtaat agtgatgtat
     1681 agagatttaa cgaacggatc ttatgactgt atgtatcaaa ataaatcaac agcatatttt
     1741 caatcaaatg cgtcaaacga cttgtttttc cgccgcgaaa atgaatgaat tgtgtatgtt
     1801 cccctttttc ttttgatgca tttatcaaaa aagaagaaag tagattattt gtgcttgcca
     1861 aatgattcaa tatctctgta gataaggttt cttcttttaa taagatgttg attaaaatat
     1921 cctcttgccc tgtgtattcg attctttgag tagcttcttt atcaatgatc accacgtcac
     1981 tttcttccat ctctatcctt tctccattaa taatttttaa acatgtaccg ctatacatat
     2041 aattgatttc aacaaaatca tgtgtatgct cgggaacttc cgcatatcga cggtgtttac
     2101 tgatatgaat atttccctgt tgaaatagat cccctcggtg gatttttggc ggtgtctgaa
     2161 gatggtaagc taagtttaaa tcattgatat tcattccttg ttcacgctgc agagtttcga
     2221 ttgattcttc gttacgcaaa aaaaatgtct aattcagcta gttccaattg actccctcct
     2281 ttttgtaagc ggtttctttc gcttattagt tcacgctgta ctgctattat atgcaaagct
     2341 atttttggtt tcataaagac tttctatggt tgggacatga acttcctgtg attccgctat
     2401 tgagagaata accacttcat actatatttt tgataagcgc ttacaaaaat gaaaggaaca
     2461 tgcttatgaa taaaaagatg atgaaatttt ctctgctgtc agtctctctt attttgactt
     2521 ctgctggtgc agtcacgtct gcaattccca gtatgtcagc tacttatgcc acgcgttctt
     2581 tatctgctgt tgaattatta acgactgctc cctcttttat ggtcactatt tttgttttga
     2641 ttagtagttt tattgctagg aaaatcggac aaaaacaaac tgtgatcatc ggattagtga
     2701 ttgcatcgat ttgtggtatc ctccccctct tctcacaaag ttacagtatt gtcttagctt
     2761 caagagctgg actaggtatt ggctttggat tgatcaattc tttggcagtc agcatgattg
     2821 ctgcctttta tcacggagat gaacgagcct ctatgattgg ttttcaaagt gcatttcaag
     2881 gatttggtgc agctttaatg acatttgttg caggacagct agtacagttc ggttggcaat
     2941 ataccttttt agtctacgcc atcagcttac ctattcttgt cttgtttgtt ttgtttgttc
     3001 ctacacctga aacaactgaa cttcaatcgc atggcacagc cccaacaaga caagtaatca
     3061 aaacgaatcc taaaatgatt agttatggta tttttctctt gctggtcatc gtggtatata
     3121 atgctgtatc gattaaatta gccacagtcc tgacgactta ttctctagga aacgaaacta
     3181 atgcagcgaa tatcttaaca atcatgcaat tggcaagtat ggctgccgga gtttgttttg
     3241 gctggcttca gacaaaagca catcgctata tgcttacact ttctttacta ctgatgggaa
     3301 tcggctttgt attgattgcc actgtgataa acctgtatgt gattgggttt ggtgcgatcc
     3361 taacaggaat ttctttttcg ttattcattc cttacttatt caaccaagta agtatccaag
     3421 cgcccaagta cgcggagagt ttcaatactt caatcttatt ggttggcgct aatttaggta
     3481 ctttcattgc accatatggt ttgctctttc tctcttttgc ttctcttggg agtaaaacac
     3541 ttgctgtttt cgtcaatggc agtatcttgc tgttgattag cgcgcttatt agccttctag
     3601 tagtacacag gacgaataga aagacagtga taagagtaga tgaagaaata aaagaacaat
     3661 tgacaacaaa ataaatttga tggaggtcgt gtaaaatgac aaatgaaaaa cataaagata
     3721 gatggctttg gtatcctgga gactttgaga tccgccatgg actgctacaa aatttccaac
     3781 gggaagaacg aggttttgac tggccagcgt actggtacat ggatgattgc catcggaacg
     3841 tcaagtttaa gagatattac tttcttgatc aaccttccat gtttaaagtg acgatccaag
     3901 gagttgggta tgtagaaatt aatggacaaa aacatccatg tggaaaatgg ctgacttgtc
     3961 cagctggtaa agcaaaaatt cgtatctttg taggacatac atcagggcta ccagcgatgt
     4021 ttatcgaggg agacgaagta aaatcagata ttggctggac agcaagtaat tttattgaag
     4081 aatatccagc tggctggagt ccgctttatg tcgatatcgc aaaagatcca aatcaaatct
     4141 attatcaaaa agaatgcgtc catccagtca ctgaaacgga gattggcgga ggaatcttat
     4201 tcgattttgg gcgagcagtg aatggctgtg tagaactaat atctctggat gaccatatcg
     4261 aatctatcac tctatgttat ggagagtccg atgtagaagc tatggatatc gattattgct
     4321 actataagca aataggaatg acaagaaaaa tgaaagctcg taaacgcgct tttcgctatt
     4381 tgtttatacc agaggtttct actggaatgg ttcaacttca agtttttcat gaatttttat
     4441 ctctttcgaa acaagctgct ttttcaagcg attcttcttt actgaatcag atctggtcag
     4501 tatcagaaga agccttcaag ttatgtagtg atttgttttt tatcgacggt atcaaacgag
     4561 atcgctggat ttggtctgga gatgcgtatc aatcattcat gatcaatcaa tatctttttt
     4621 ttgacgagga aatcaataaa cgaacgatat tagcattaag aggacaaaat gaaatcaagc
     4681 agcatatgaa tactatcgtt gactattcga tcttgtggtt gattggtatc gaaaaccact
     4741 atatgatgac gaaagactat gaatttttac gcatcgtcta ccctaagatg caatcattaa
     4801 tgagatactt gatggagcaa acgaatgaat tagggtttat ttatgggaga gcaaaagatt
     4861 ggatatttat tgattggtct gaaatggata aagaaggaac gcttgctgca gaacaagtct
     4921 tattactgaa agcctatcat tccatgatca cttgcggaga tatcttagct gaaacatatg
     4981 gagagaacga aaattatcat actcatctct atcaagaaaa atatcaacta ttgaaacaaa
     5041 acctatttga ctacttttgg gatgaagaac ttggcgcttt tatcgattgc tatgaatctg
     5101 ggagaagaaa tgtgacgcgt catgcgaata ttttcgctgt tttattcgat tttgctaaca
     5161 aagaccaaca gcaatcgatc ttaaagaatg tcttattgaa tgacactatc acacagatta
     5221 ctactccata tttcaaattc tatgaacaag atgctttatg caaactcgga cagacgaata
     5281 tcgtctacca aacgattctt gattattggg gagggatgtt atctcatgat gcggtaactt
     5341 tctgggaaga atacgaccct actcaaacag gaaatgaaaa gtacagtatg tatggagatc
     5401 cttatggaaa aagcttatgc catgcatggg gagcaagccc aatctatctt ttaggacgct
     5461 attttatcgg tttgtatcca acaaagccag gttatgaaga atttatgata caacctaatc
     5521 tagattcatt tgaaacactg aattgtacgc tacctatcaa agatggaaag atttcgcttg
     5581 aaaaaacaaa agataagatc gtgattactt ctgatagaac aggcggaaca gtgaaaatga
     5641 atggtaaaag ttatcctctt ctagcaaaac agccacttgt cttaacaagc tattcaaaaa
     5701 taaatgcata aaacaaaaaa ctgcttgcga catatcagaa gactttaaat cttcttctga
     5761 tattgtccaa gcaattttta ttttgaagtt tgcttaattc tgttctttag aaacaatgtt
     5821 tgttcttttt ccggcaaaaa gaaagctaga taatacgaca agtaaaaaga aagcgatgat
     5881 tccaactgtc aaggctattt gaccgtattc tcctagtttc ccaagtacaa gtcctggtac
     5941 tagataatca gtatcggcaa atgtagaaga agcgatttta agatctccta atactggaag
     6001 gataaataat ggcagccaac taatcaataa gctattgaaa gcagaaccta ataccgcccc
     6061 tcttctaccg ccagtcgagt tgccgaagac accagaagca gctccgcaga agaaatgtcc
     6121 tactacccca ggaatgataa ctgtagtttg caagcctatc atagcaaaca tggatagtgt
     6181 tcctacaaag aagctaacaa aaaagccaat taatacagca ttaggtgcat atggaaaaac
     6241 gataggaaca tctaatgctg gtttagaatc gggtacgagt tttttagcaa tcccttggaa
     6301 tgctggaacg atttctgcca acaccattcg aaccccttga aggacgataa caaacccagc
     6361 tgtgaatgtt ccagcttgga tcagtgcaaa gattacagcg ttgtctccat tgcttagttc
     6421 tctttcaaca aatccaggac cagctaacaa agcaagtgcg acatatacga tcgacatcaa
     6481 gagcatgatc gatacagtag aatctcgcaa aaagctgaaa cttttaggaa tctttatgtc
     6541 ctctgtagaa cggtctttgt ttccaaaaaa ctttccgatc catccactag aagcataacc
     6601 gatatttccg gtatgcccta acgcaacttg attgtttcca gtgatgttct tcataaacgg
     6661 ttgacaaaga gcaggcgtaa tagttaatag tgttccttcg aagaccccgc ctaataagat
     6721 catccacaaa tttgcagtaa accccgcgct tactaaaatg actgctgtta gacaagaaac
     6781 ataaagcatt gcttgacctg taagaaaaat gaatttatat cttgtgaatc tagcaagtaa
     6841 aatattcacg atcatcccta ctagcatgat caaagcagta ggtgtaccgt attgttgcaa
     6901 agcaatcgcc actactgctt cattgctagg aactactcct tgagtatgca gagctgtttg
     6961 gaacatcgaa gcaaaagggg ttagtgaatt actgattaaa ccagcaccgc cgatcagtac
     7021 taggaatccg acaaatgttt tgatagttcc ttggatcact tctgaaaatt tcttcttctg
     7081 aagaacgaga ccaagcagtg caatcaaaga aacgaggatg gagggctgac tgacgattga
     7141 cacaaacaaa ttcaacaatt tatccattga tagttcctcc cttgaatcat ttttattctt
     7201 ttgacaagat cttcgttact ttttctttta cttcttcgct gtcaatgatc gattgcaaag
     7261 gtacgagctt ttcatcaggt atagaagtta gcgtatcttt caaagaatgc tcaatgaaga
     7321 aataatcagc catatcgcta gatacacttc caacatccgt atgacttact tcaaactcgt
     7381 ccacatctgc tccaagatct gcaagtgctt gtttgatgtt catctctacc ataaaacttg
     7441 ttcctaatcc actttgacat actgcaaaaa ttttcatatc attcgtctcc ttttcttatg
     7501 atcgctaata tttcttcttt tgttgatgcg tgtaataaat cttgaagatt ttgtttatta
     7561 gaaagtagct ttgttaaatt tgccaaagcc cgaagatgtg cctcattatc gatagcagct
     7621 aaacagatga acaaactaat gggatgggct ggattatcaa gcagatccac aggttgttga
     7681 agtttcagca aagacatccc tacttcgttt acgccatctt ctggacgagc atgaggtaat
     7741 gcgatgtgat cgccaatatg gataaacgca ccatactgct ccacgcgctc gatcatagct
     7801 ttgatataac tttcttctat tttattttct gataatagcg gctgtgcggc aaatgtgatt
     7861 gcttcttgcc agttttttgg ttgatccgat aattggatca tttcttctgt taataattca
     7921 gatagcatag gacgttgatc ctccttcttt tctaaaagtt tgttcatttt acgatttaat
     7981 acttgataga gcttttcttc gtctacgcct tcttttaatt cgatataagg cagtagcgcc
     8041 gacaacaatt cttgggcaga aggcattgaa aatcctggta tcagccaatc tctttgtact
     8101 tgattgatca gctgattttt ttccaagtca gtcatgattg gatgcagaac atagagagac
     8161 tttttcttat cagtaagtgt gagaggtaca gtagaaaaaa taatatcgta agaagtttcg
     8221 tcaacctcgt gtaagcggtc agctgaatta gtctctgtaa aaaagatcat cgggaataat
     8281 tgctgaagtt cactttttaa gatcaatgaa gagctaatgc cacttggaca gacgatcacg
     8341 gctcttattt ttctatttct ttcttctgcg tccgcttttc tgatctctcc gccaaatagt
     8401 atagtgaaaa aaccaatctc ttcttcaggg atgctcttcc ctattcgtct ttctaacgga
     8461 agtaatgctt ttcttgtcat ttcgtagatg gaagcatatt gatgcttaat ttgatcgatc
     8521 atcacattag gtaaataaaa agaataggaa atacgaaaat aggcagggac caagtgataa
     8581 aagacattca tcaataattc acgaaacgaa gcaaattcta cggccatcag gaattccatg
     8641 cgatggatga cttctgaaga acattccagc aaaaaatcaa gtgcaggctc ctggacatct
     8701 ccttcaacga tcgatgctaa gcaaagagag aaaaatataa tctccgaatt ttgatgttcg
     8761 gaatagccaa ggccagtcaa tacttgtcga ctaatgtcag tgactgcaag agaagccaac
     8821 agttcagcat cttttaccag ttccatggat gtttttccaa ttcgaatggt tgtcaaactc
     8881 aaataataag ctaattcatc cattcgattt ggtgcgaatc gaagatctga tagtgaaatc
     8941 gttttatcta gcaaatctct aactttggcg taataatcaa tggaaagggt tgcggttaaa
     9001 tacgtgagcc caaatcttcc agcaagtgac tgcatcaaac gagcagtgcg attttttgct
     9061 tctcttctta atattgcttc tgaccctagc agcctaaaac cagttttcct agtatacgac
     9121 agctgacaat cttccacttc taattcattt cttatctttt tgatatctga caggactgtc
     9181 cctttagaaa cctgaagaaa atcttgaaaa tgaaaaacag aaacagacgt tgcagtcgaa
     9241 taaaccatca aataaagaag atctcttcgc tcttgttcgg tatacacagc ttcaaatcga
     9301 gtaatatcca aaaatttttc tgcccagtcg attgctcgga atttctttat acggatacgg
     9361 gtatcatcca catattcggc aaaaggagtt aattgtttga tcgcctcgct tgtatttact
     9421 tcgatgatct ctgctgcttt ttctagactg atttctcttg gtgttttcag gagataacta
     9481 attttgtagt tcacagcgct gttcacctcc tacttatatt ctagtatggt agcgctttta
     9541 aatcgagggg tgtttggtac aacttacagt tggatttttt ttacgactgt accgaacgat
     9601 ttctatcgaa tggatacaga aaaaaagcag gtgaccaaat gtctcctgct tttatgaatt
     9661 gatttcgtcc gatcaatgat gatcatgttt attttttgag gtcagttctt caaatttttc
     9721 tttgacatct tcaaccgctt tttcgatatc tttttcaaag tgattgggct cattaggctt
     9781 tgtttcctct tctacagaat ttgaaccaga taaagctgtt tctttatttt ctgtttcacc
     9841 agagtttgtg ccagctaatg attcttcttt gtcttctgtt tcaccagaat tagttcctgc
     9901 taaagccact tcttcttctg ttttaggggc cattgaacct cttaatcgtt tgtgtttttc
     9961 accgtcataa tttgtcatgt tggttcctcc tagtagttct atactttttc atcgtacaat
    10021 aagacagttt atgcgtcaaa aaacaaaccc gattcttaca aatatgtcat atttttcctt
    10081 ctgattcttg aatgcttttg attccttctt ccaaaatttt catttgtaag attgtttctt
    10141 catctttgat gactttttct gcgccattaa ctaatgtctc gtaaatccct tcgtagacac
    10201 ggctgtaatc accaacctcg gaaattactt tttcttcatg ataactccct tgttcatcta
    10261 tataggtcaa tacaccataa tgttcgggca aatcgatacc aaagtcttca tgatcaggca
    10321 tgtaaaacat tttcagatgt tcttcttgtc gatcttttgt ttgtttcacg aacatccctt
    10381 tttttccgta aacgacgaaa cttggacgtt ctttgatgcg gaaataactg gattttatcg
    10441 aaattttcat atttccatag aaaaaatcca gatcgaagta atcattcatt cgtcctttcc
    10501 ctaaaagttg acgtacatca tagctgatat catctggttc gccgaaataa gaaagaactt
    10561 gatctactgt atgacaagca tgtccatata aatagctgtt cgcagtagaa aattcatgcg
    10621 tattttctgg tacttccggt ctgaaataat cataatgcat ttctacttct aaaatatccc
    10681 ctaattttcc gctttcgatt actttttgga ctgtcaagaa gtcagaatcg aaccgtctgt
    10741 tttggtaaca ttggataaat agaccttttt cttttgctaa tgcaaataac tcttctgctt
    10801 cctttgacgt ttctgcaaaa ggtttctcta ctagtacatt cttaccatgt tcgagtgcca
    10861 tttttccata ttcataatga aattgactag gtgtagtgat gacaactaat tggatctcag
    10921 gatcatccca aagatcagac agctgatcag tatattcgat gtcttcgaat gttttccaca
    10981 ctggttcaga tggggaaaaa atttttttga tattgatttt gttcgtaagt tttcttgaaa
    11041 aaggcaaatg atagcgattt gtactctttc cattgccaag ataagcgatt gtaagcatga
    11101 gtggtgcctc cttttattga tgttttcagt atatcctaga aaagcgcaga aagtatttat
    11161 aatagctatt ctggtaaaaa agttcagaaa aacacttgtt atcattcggt ttggtgaaat
    11221 taatgttcaa agtaaggata aaaatcaaaa agatagtagc ggaaccaagt gtttatcagt
    11281 aagatagaat ttggaggtca gatcatgctt attgaagaaa aactaacaaa acaagagttg
    11341 tttaccacga cagagaaacg gattgtcgat tacatccgta ggaatatcga agctgcagtc
    11401 tacatgacga ttgaagaatt agcaaaagct acgtatactt cccattcagc tatcattcgt
    11461 ttatgcaaaa aaatgggatt cagcggattt aaagaattcc gtttcgagct ggggcgggaa
    11521 gttcatcaat tgattgctca gttcaatcaa acgattgatc ctaattttcc tttttcaagt
    11581 gaagatagca ctcagcaaat tgcaaagaaa atggcggatt tatcgatcca gtcaataaaa
    11641 aaagcacaat tgcagattga acatgaagat ttagatcaaa tcggtaaagt attgaacaag
    11701 gcacagcgaa tctttctttt tgcaaaaggt gattctcaaa tcacggcaag gaaattccag
    11761 aataaaatgg tcaagttgaa taagtttttg atcatggctg aagaatacag tgattcttct
    11821 tggaatgcag ccaatttgac tagcgaggat tgcgccattt ttattagcta cagcggtcgc
    11881 atccatcatt atgaacgaat aatgacctat ttgaagcatg tagaggcccc taccttgctg
    11941 attaccggaa atcagcattc agaaatggcc aaacaggctg atatgtgtct tgttatatcg
    12001 caggaggaat atgattttgc taaagtagcg actttctcct ctcaaattgc cttcgattac
    12061 gtattaaata cgttattctc agtgatatat tctcaaaatt ttgaagagaa tatccgcaac
    12121 ttgaaaaaca aacaagaatt gatgcaaaaa ggccttctta aagaatatcg gaaaacttaa
    12181 gaaaagatcc tcatttagta ataagaaaga tagtgtttga taggctctct ttgatatcat
    12241 cataaaaaca ggctgaagtt ttctttttca aacctcagcc tgtttgttta cggaacgatt
    12301 cgaatagata tattatagaa atgaggaata gctaacttct gtttcttatt ttgaacgatt
    12361 gacgaaaaag atatataaga aggaggaaaa tcagtataca tgcagctaac aatccataca
    12421 ggaaaaatcg tttgttctcg gatactttct gtacttcttt ttgaacttct tttgcatgat
    12481 aaggaacccg gtgccctctg acaagtaagc gatgactgtt gaccatataa ggtgtacaag
    12541 ttaggagtgt cacgaaatcc tgcccttttt cgattctcag atcttttgta tcagtcggtt
    12601 ctaccgtctt gatctgatcg acttgataag cgagtgtccg tccattgatt tcgatataga
    12661 aagaatcacc taaagtcagt ttcggtaagt ctgtgaacaa ctttgcttga ggcagtcctc
    12721 gatggctgga aataacggca tgtgtatttt ctccccccgt aggataagac gttccttcca
    12781 ataatgaggc acctttttct agcagcacag tattcgtttc atcaaaaatt ggcaaatgga
    12841 cattgatctc agggatcgtc aaaataccga tcgtatgttt ctcaaaatag gttctatcct
    12901 ttttcgcagg ttcttttttc ttagtaaagg gatctgcacc tggatttcca ccttcttttg
    12961 ctaattgctg gtttttcttt gtcatgtttt cttggatttt agccatgact tcttcatttt
    13021 ctttgaccgc ttgttgctga taatgagaga tgatctgctg atccagataa ttattcagcg
    13081 cgtcgctaac aaatggataa gctagtatcc ctattcctga gagaagcaag aaaagcatca
    13141 gcagatctag aagtttcctt ttctttggtt tcatttgtgt tctccttgtg atgactgttt
    13201 gataataaaa gaaaaaggag catttgcgta atccgctgct cctttttcca attcttgcaa
    13261 aaagactaaa tctgactgtg cttgcgtcta gcaaagtaca gtccagcaat cagtagaagg
    13321 actactcctg caccgatata cacatagatt cccttaccgc ctgttgaagg aagtgtacct
    13381 ttgtgtttat ttggtatttt ttcaggagaa atcaactgtc ctgctgttac ataagattgt
    13441 tcatcgatag taaatgctac acggtttgtt aatggaacat atttttctgg cgctttcgtt
    13501 tcttccagat agtacgtgcc atattttaga cctgtcacat cgattaaacc gttacttgtg
    13561 gttgtaaaaa ccgttgctga ttctttcgcc gatacccagc tgacggcttt tgtggatggg
    13621 tcgatcgata aatatttcgc tgtgtcacta tcttgatcac gaacgacgaa ttctgctcca
    13681 gcaagtgttt ggtctgatgt aacgtcacca tctactttaa caaatcgttt gcccccagta
    13741 acgacatcga ctgacggtgg tgtttgatca tttgtatgcc cgttatcgac atttgcttgg
    13801 ttagaaaatc ctttggttgg atctgctttt tcgttcaaat gcatatagta aacgaatttc
    13861 aatgtaccgc caggagttaa tgaaggaata taattcggat caactgaaac cgtgaatccg
    13921 tctgtttgct cagtgacaga ataatttact gggtcgattt ctttatttcc atcatataag
    13981 gcataagcat acgttcctga agaatcatta tcaaatgtta aagcagcgtc atgagtatcg
    14041 ataagtttga atgttgtgta cttgttttgc gttccttctt tatcagcgat acctaatggg
    14101 atattgacag aaatctcata ttgaattttt tcaccgattg cgacatcttt tccattcaat
    14161 tgaggtgttg ttttatcaac tttagacgta tcatttttga tggtcttttc tactggtgtt
    14221 tggctatttg ccaggatctc aaaaggcgtg attgtttgct tttcaatcat ttccgcatta
    14281 tcaggagctt ttacttcttc taaattatat tttccaactt ctaaatgatt aacgatcaac
    14341 tggccttttt caatagatgc ctcggcaaag tcattgttgc cgatgtcata agaatgacca
    14401 gtaatgaaat gtttggcttt ggtttgatca gttgtccaag tgtacaatcc atctgtgaca
    14461 ctttggatgt attttttgac gcttggtgtt ccttcttctt tagaaataat aaattctgct
    14521 ccatttagtg cttcgttttc ggcagtaccg atttttgtaa ctttcaacgt tccatcatta
    14581 ccgactgtat ttttagggta gagatggata gtatctagtt cttctgtccc gtatttataa
    14641 gagccatctg cttgtttgat catctcatat acagggaaag ctaaaaccat gtttgcggca
    14701 gctgacactc cgtcttttgg ttcttctttg atcgtgtaga ctgcatcttt cccattttgt
    14761 tttttaggta aagataaagt gacatttcca tcagcatctg tcgttcctga agcaactggt
    14821 gtaccaggag tcaaagactg gactgcttgt tttgctgcat ccacggacgc tcctttatct
    14881 cgttgcgcat aaaattcttg agtgacgtta taaactgaaa atgaaatatc ggctaatcct
    14941 tggtattgat cgaattcgct catttctttc ccgctgtttt ggattaaagg atcgggtaaa
    15001 tcagtcattt tctttttatg aaggatgact tgagctgctg tctcctctgc aaacactttt
    15061 tttgcgccga agctgtttgt gagtaatggt aaaataagga aaaggactcc taacataacg
    15121 tttatttttt tatgattttt cattttttca ctcctgttct ttttttgata aacaaccaga
    15181 ttgcaaagct tgcgaatacc ataccaacaa cgcctaaaag tgtatttttc gtttcgtttg
    15241 tttttggaag aaatggacgg ctattttctt ttttattgac tactttcaat atactgtccg
    15301 cctctttatc ctcagaaaga aaagttgaaa tggtaaacgg aatttctgtt tcacttaaaa
    15361 tataaccaga aggtgcttcg atctctttta atcgataact tcctgttttc aacccggaaa
    15421 ttgaaaaagc gccattttta tcggaaatca ggtgaagcgc atctgattcg ttctttgtcc
    15481 acttgtatcc gttggctgtt tgctggaggt aatttcctgc tttgttttta ataaggaata
    15541 ctgcgccagc taatcgttta tctggtttat ttgtctcgat tttgacaaat tgcttactcc
    15601 ctgtatatac agagactttc tttgtactga caccaaaacc aaaatccagt gttccgttgt
    15661 tgttgatttc cttgttcgcc tgtgctgtac tgcttagctg catctgataa gaaatggtca
    15721 gtttttttcc agcatatttt tccaacgaag ggatcgaaaa taaaacatca aatccgtgag
    15781 gtttcttgtg tatgacaaag ccttctgtca gcttttctcc attcgatgaa atcgttaaac
    15841 tattaggtaa aaacgttaat gcttcatccg cactatctga caattcgaac ttttgatagt
    15901 caaggatatt tacaggaatt gtcgtatgca aggaataagt gattttttca ccaatcgtaa
    15961 aatcatttct aggctcttct aataccttct caaaaggtgg atcagggtag tcgttttctt
    16021 catttttagg atacaaatgg atagttgaaa gtttttgatt gttttgtcca tgtacaggaa
    16081 gaacaactac catattctct gccttttctt tgacgacttc tggtactttg gattcaatga
    16141 aaagatacgc tttatctctt ttttcttgat ctttagcggc cagagaaaaa gaagcgatac
    16201 catcttcgtt gttaagagtt gtagttgttg cttctgcggt aaacataccg gaagacgcac
    16261 cgttttttgc gatctctgct tgtgcttctt ctaccgtttt gcccttttcc cgtagatggt
    16321 aaaaagaatc tgtgacatca taaacttgga atgtgacacc atttaatcct cgatacgttt
    16381 gtaataaagc tttttcttgt ccgtcatttg gatgattttt cggcagttgc ccgtttggga
    16441 aaagcaattt gtgtaaagta atctgaacca tttctgtttc tgtttctgtt gctacctgag
    16501 taaaagctgg tttaaatagt aacaagaaga gacacatact aagccaacca agttttttca
    16561 tatttagctg ccccctcttt cttttttcaa aaaaagatac caaatagaaa acgcacaacc
    16621 aatcatccct actagataga ttcctaaacg gccaattcct cccgtttcag gtaatggtac
    16681 ttttgcctga ttcgtgatgt ctaaagaaat ctggttgttt ttggctcctg gtgacagaac
    16741 agattcatga tcttgtccat ccacttgaat tgacccatct tcgtgtataa ctatagtaac
    16801 tggctctttt agaccttggt atccttctgg tgtaaaagtt tcggtcagcg tataagttcc
    16861 aggttttaaa ttttcgaata gaaaggtatc tgtttcttgt ccatcttttg gcgattctaa
    16921 gtccatttct ggtccctgca gtctgaattt tgctcctttt aatgtctgtc cgttgtcttc
    16981 ttttttgtgt accgttagat caaacgcttt tagtcgattt tgatgagtga gcgtccatac
    17041 attatttgtc atctcggtat tttttcccgt gtaaatgatt tttccatatt tatcgatttg
    17101 gaattcataa tttcccggcg aagtatcata tccggcagga ccagctgttt caacgactcg
    17161 atatttacca ggtgtgagtg aatcaaaact ggcaagacct ttttcattcg ttgtttggct
    17221 gtcaattggc tgataagtac catttgcttc ttttttctgt agggaaaatg ctgctccttt
    17281 aagatttatc tctttgtccg tcccttgcat cgcatactta cgtactgcta caggaacttc
    17341 aacaaaagga ttttcaatag tcaactggat cgtatcatcg gaagtagtga ctgtttttcc
    17401 atcaatggtt accgtaccat cagaagcgat cttgatttcc caagtagtct ttttgcttaa
    17461 tccatgccct tctggagctt ttgtttctgt cagcgtatag gtcatttctt tttgcaattt
    17521 gacattttct ggaagagaat aggttccgtc gccatgatct gttagtaaag tatcaataga
    17581 atctcctgtt aatttgaaaa cagcgccaat aagatccttt tcacctgtag aggaattttt
    17641 cgttattttc aagtttaacg gtgtgaattg tttagtattt ttccatgtca ttgcatctat
    17701 ttgttgagaa tcgtatcctg gtacaggtaa ttctttgatt gtttggtaac tgaatgcttg
    17761 accttgatta ttgtattgag gtagtgataa tatctcttga taactttctc cgctatttgc
    17821 agataatttg tcaatgtctg cacgttccca cgtatttgtt gtatctttag ctggtttaat
    17881 gattcgaata tatccgtttt tccaagctgc agcatttgtc gtatgttccc gttgaatctc
    17941 aaaagtaact tgatctggac gatcagctag attgttgtca aattcttccc ataacttttt
    18001 gatgtgaaga ctgactccgg gagctttagc agatggtata ccgaattcag ctaattcatt
    18061 ggtatcaatg tttggctgga aagttgtcct gccgttcatt tgataccaga aatttggatg
    18121 gaagtcctca ttttctgttt ggattctcac ttggtaatgg atttggattt cttggtcttt
    18181 tcctaaatag atctgattgc tcttgatggt atttccttct atggaaatag atggagtgac
    18241 cgttgtagga cttgtcccca cactcttgac tgaaagtgta ccaggctgat aaatgaatgg
    18301 ttctgcgatt ggatcattta tttgtccatt gcttacagtt gctgagatct gtacagcttt
    18361 tttggctagg tattcagaga tatctgttgc atgatcagct gattcatagt aaagatcgcc
    18421 tttttcatct gatgaaacca tttgtcgcat acgagactct acttctgctt ttgagagacc
    18481 agctgccgga tcgctttgaa gttggatgcc aagaccatgt atttcgattc ctcgttcttt
    18541 gagtgccatc gcttctccga tggttgcgat aaacgtactg tcgattcttc tggatagatt
    18601 gttttggtca ggtgcatcat agattcttga gattagagaa gtatttcccg gccgatcttg
    18661 cgtattagaa aactgagttc cgtaataatt gctgcttgat tgtgcgtgta cccgctgtac
    18721 tttataggaa aatgttggta caccatccgt cagcaaaacg atcacttttt tatgtccatt
    18781 tggaacggat agcatgcttc ctgcatctct tagtgctttt tgtgtaaaag taccaccatt
    18841 tgtccgtgaa ggtgtaatgg attttacttg atttttcact gaatcaaatg aacccatctg
    18901 tactgcaccg ttactgtagc tatatccttc gcttgagtag ccgacatatc ccatattgat
    18961 tttgtctgtg ataccgctat ctgctaaagt atcgacaaaa cgatcgacac caatctttac
    19021 ttcaccgatc cgattattgt cgttcatact tcctgaccag tctacgacca atacgagatc
    19081 aagaggcgtg atatcttttt gtacatttcc acgagcattc aaataaacat caaacaatcc
    19141 gggtgtactt gtttcctttg catactttcg tatcgcatag tctgcttgat taccggttcc
    19201 tccatattcg atatacgaat gggtccgatc attaggatct ccattccaag aatttatacc
    19261 atcccattga tttgttccgt ttttattgcc ttgatgatca agaacatttg tatttcctgt
    19321 aggttgccaa ctggctttag gataagtgcc ctgatcatct gttgtatatt ctgtaggaat
    19381 cgtttgatta gatggcacac tcacagctgg caaggataat gtaggcaatg taaatgggct
    19441 gacagaattt tcgttttctt ttgatgattc ttcttctatt tcgtctggct ttggtactga
    19501 cacatctgta tctttcttat tttctgtctc atcaataatg tcatattctt tattatcgac
    19561 gatcagcttg tttgcctggc tgtggtaaag tgctggatcg attggaaaac tcagatcgat
    19621 agtttgttta gtgtttgatg agatttccaa cgtaattttc ccgctattta cctgataagc
    19681 atagccatct ccttcttttc tttccacatc acttaagtaa ttttcgacta gaccaatttc
    19741 tttttctatc ttttcagcac tggtgttatt cgcatttagc ttgatgttca gtgctttttt
    19801 tccagactga tccgaaatga cttcagctga aattgtttct aaaactgttg gatcatctgt
    19861 agtgtctgct attgcagata atggcacaaa gtttgataaa ctcaatatta tcagcatgaa
    19921 aataactttc agtttcttcc ctgtggtcgt catttttccc ctcctttttc tttttttctg
    19981 ttttgacgtg aatagtgaaa attattaaaa taaacaggcg ttacataatt ttatttattt
    20041 taatatgata attattatac gaagggcgct aaagggaatc acaccaattt tcataccaat
    20101 tggtttcatt tatgaagttg attccttcga ttaatgagcg tttcattgaa agaaaagggt
    20161 tgagggagaa aaacatatga tcaggttgtt ggaaggaaac tatagaaaga tggaaaaaat
    20221 cattcgaatt ctgtccattt ctgacaagtg gatgactaaa aaagaacttg ccaaacgaat
    20281 tggtagttca gaatctacat ttgtaaggta tatcgaagag atcaaaatcc gctggggaac
    20341 ggatgtaacg attcaatctt cacaaaaatt ggggtatcgt cttgaacatt tgaatgtacc
    20401 agcttattta aatgttctga tggaaatgtc gcaaagttct acaaacagtc gtttattaaa
    20461 tgagattatt gtcaatccgg gtaaaacgat tgagtattat tgtgaaaaaa tttccatcag
    20521 ccggtcttct tttacacgta agttgaaaca atgcaatcaa attttagaaa gctatttatt
    20581 aaaagtgatc gttgaccaag gttttcagtt aactagcctt aaaagtgagc tccagttgcg
    20641 tatctttgtt acctttttct ttttaagtta ctacggaaga cgcacgttgc catttccaat
    20701 cgaaaaaaag aagatcaaag aaattatggt caagaataat tgtcagatga atttgtttca
    20761 ggaagaaagc ccatatgagc aatctttctt tattatgtat tttgttgtgc atctcatgag
    20821 agaatcccaa ggaactccat ttgaatggaa agctggagac tcttatgtac agttgataaa
    20881 tatcgataaa aaagacttct taatcttgaa aaatctctta ggaaaattga agtacagcag
    20941 ctatataaaa gtagtgaact attttgtcaa tacgattttt tcagaatttt cggagaaaga
    21001 aaaaaatgaa atcagtgagc agatacgaaa aaatatgtcc tactgttcgt tttcatccgc
    21061 tgattgcaaa gaaaaaaacg aagaatatgt tgttcaatta ttaacgcaaa tttatttttt
    21121 ctcccgtatt ttcccatacg atacgactca tttgacgatc cgtgcaactt actttgcaca
    21181 gcagatgcaa caccgccaga tacgtctata tgctggattt cgtgcagaac ttcaatttta
    21241 ttccactcta ctgaatcaga atctgattta ttatttatct agctatatct tttggatgct
    21301 tatcggtaat cctgaaattc accattatac ttctgataaa aaaatactga ttatcagcga
    21361 cttaagtctt cgacatagtc aatatattga agaatatatc agtgatattc ttgctgtgca
    21421 taaaattcac tctgagacaa ctgctattac tgaagatcag ctgtcgaaat acaatcttct
    21481 cgagtatgat ctgatcgtta cgaatcaacc cattttgaat gcccatgttc ctcatatatt
    21541 gattgatgat agtgtgtcat tcgcaaatga agaagaactc atccgcttat tcgagttatg
    21601 aataatcaaa aaacagctat gtataaacca ccctgataaa aatttcagga caaatacaca
    21661 tagctgtttc tttttattgt attccttcag caatccaacg agatttatag cgaaagtctt
    21721 ttaacaactc ttcttttgtc caattatttg caatgagatc cgtcaaagtg ttcattacga
    21781 gggtcatgga aaagtatagg aaactaactg cttcttccga cgattgaatg gagaaatgat
    21841 tgattcttaa taattccagc catttttcgt tcattggaga atcagccgat agttctctac
    21901 gtttggttgt ctgctgatca tttcgctggg ttaacaagta taacctttgc caatagatac
    21961 tttcgtgatc taagtcgcag caccactcta aatatttttc gatccccaaa aagaaatgtt
    22021 gtttgttttc gctaatgaat tttaagatat cttgatggac tatgtgagaa ctgcttttga
    22081 tcgtatacaa ataagcatct tccaaatctt gaaaatattt ataaaaagca ccgcgcgaca
    22141 tccccatctt gtcaacgatt tctgagactt tgacttggct gatgtgctgt gagcaaaata
    22201 tctccagtaa taggtcatcg attctctgtt tctttgcgtt cgataaattg aaaaaagttt
    22261 cttttggcat ccacatctct cctttatctc tgattatacg tggatagccc ttcattgtca
    22321 tgaaggattc attccttctt tttctcttct tctgtcaagt acccatcttc cattcgataa
    22381 acgcgatcac tccattgaat catacggaca tcatgagtaa ccataatagt cgctttttgt
    22441 ttctcatgag cttctttaac tagtaatttg acgacttcat atgcatgatc tgtatccaaa
    22501 ctagctgttg gttcgtctgc taagatcaag cttggctcat tatataaagc gcgagcaatt
    22561 gccactctct gtctttctcc accagaaagg tctttaggat aactattttt aagtgactgg
    22621 atatctaaag attgcagcaa ggaatcgatt tttgttgaat cttctttttt tgaagtaact
    22681 tggtcaacta gttcaaattg ttcctttacc gttaaaaaag gaatcaaatt agagctttgg
    22741 agaataaatc ctatttcttt aaagcgtaaa tctgctcgtt ttttttctgg aagctcagtt
    22801 agtgagtgac cgttgatcac tatttctccg gaagtcggtg tttgcaatcc tcctgaaatg
    22861 gtaaggaaag tacttttacc tgaaccagaa ggaccaatga tgctgacgaa ttctccttcc
    22921 tttacataaa aatcaattgc tttcaaagct gagacctttg tatgaccaga accaaatgtt
    22981 ttggtaactt ttttcatcgt taatattttt ttcattttta ttaacctcct attgctgtaa
    23041 ttgggtctac tttggttatc gtacgtatcg aaaacaatcc gcctagcatt gcgacaatga
    23101 tcaaaatccc actatatagc aaccattgtg accaaaaaac agcaaaaggc atcgcgcttg
    23161 gtaaaatcag actagtcaaa taagcaaaca aaagagcaag cagaacgcct accaccccaa
    23221 caataaagga ctgtgctact aaagatttag cgatgaagaa atttttgatc ccttgtgctt
    23281 tcattactcc aaaaatagca gtcttctgaa gtgtcatcac gtacatgaaa ataccgacca
    23341 cggcagcaac cactaagaac aaaaagtata tcatcgcatt taaggtcata ttttgggcag
    23401 aatagccagg aatactttca atgaatgtcc caatagttaa taactgtaga cccttcgatg
    23461 ctttttcttt cgaaatttta gctgaatcct ttattacgat cccattgatc ggctgttctt
    23521 cttcagaagc aaacggctgt tgtccatact tcaaagcagt ccacgtatct agggacgcat
    23581 agatgacggg agaaactgtg taataagttt caggaaagat accgacaatc gtcaaaggat
    23641 cgtcgtaact gccaattttg atcgtatctc caattttata gcctgaatca gccagatttt
    23701 gagagatgat gatttcattc tttgttttaa attcatgccc tttcgtgagt tttggtaata
    23761 aaaatgcttc tttcgttgtg ccgaaaactg taacattttc tttgtttttt cctttgattg
    23821 ctccgctgta caatccgatt ggtgctttct ctcctccttc aatgtatttc agatctcctc
    23881 ttgttaattg tgaagctgca aatacttgat ttgcatcctc cgacaaaaca atctcttgtg
    23941 cgtcccaatc atcgattgct tttttaaact cttctgaaag tccattcgct agtcctgaca
    24001 acatgaatac gacatacgca atcaagaaca taataccgac gatcaagcca tatcgtaatt
    24061 tagaatagcg aatttctttg atagctagaa acatttgttt ttcctcccta tgatagattg
    24121 atagtgacaa aatgtcactt gttgaatagt gtaactctaa agtgacaatt tgtcacttgt
    24181 gttgtttttt tcttatcaat aaattgtcta agacatcttt cgataaaacc ttaacatgag
    24241 tcataaaaaa tttatttaag ccttatttat gctttccatg ataaactaaa gtgaccaaat
    24301 caatgaggtg ggaaaatgat taaactatta gtagtagaag acgataaagt attatccaat
    24361 aatataaaag aaatactcgc tgcaatcggc gagataacac aagtttatga tggtagtgaa
    24421 ggactgttcg agttggaaag tggagtttat gatcttgcaa tcttggattt gatgatgccg
    24481 gaaatgaatg gatatgaagt gttagcaaaa atacggaaag aaaaaataca gactccagta
    24541 ttgattttga ctgcaaaaga tagtttggaa gataagattg aaggatttga acgaggtgca
    24601 gatgattatc taacaaaacc tttctatcga gaagaactga tcatgcgcgt gaaagcatta
    24661 ttgaaacgct cgctaggtct ctttgatgaa aatgttctga agtataagga tatcagttgt
    24721 gatttaaaaa gcaatgaagt cttgtataaa gaacaagttt tgcccataca aggaaaagag
    24781 tttgaattgc tcgtttattt tttacaaaat aagggaatca ttttgacaaa agagcagata
    24841 tttgaccgga tctggggatt tgattctgat acgacgatta cagttattga agtttatatg
    24901 agcaacttac gaaaacatct gcgaagtact ggattggata aagaaatcaa aaccttacgt
    24961 aatgtcggtt atatactgca aagggtgtga tgcaatgaat ggagatatta cacgaaagca
    25021 acgtagtcgc ttttttgcga caaatgtagc agcatttgct tttatctttt ttcttttggg
    25081 attcattact ttacagatat taaaccaatc tgcatacaga gaaacagatc tgtcattaga
    25141 gaaaataaat tttgattctc gaacagtaca attagagata caaagatatc agcaaggcga
    25201 tcccttttta aacccgactc aaacggaaca aaacagaaca aacatggaac agccattaat
    25261 ggaaccatca aatgaccgtt ttaacactca agtgatcttg tggtctaaaa atggtgagat
    25321 attgaataaa gcagctattg gtgggcgttt caaccaaatc aaagaattaa aaatggatac
    25381 gaaaaatctg aataccatcc aaaatattga attaaatgaa gacagtggct catctgccct
    25441 ctctttccgt tcgattacga aaaaagcgga gacgaatcaa gatggcgttg cctacatcca
    25501 aattttagaa aatacaaatc aaatcaagaa ttcattagcg acttttcgca cgattatgat
    25561 tttgtgtatg attgtttttt ggattctctc tattggtatc agttattact tgtctagtct
    25621 aagcatgcgc cctattattc tttcttggag aagacaaaaa gaatttgttg agaatgcttc
    25681 tcatgaattg cgcacaccgt taacgattat tcaaaatagt ttggaacatc tttttaccaa
    25741 gcctaatcat actattattg aagaatccga aagcatcgct caagcattgt cagagacgag
    25801 gcgcctcact ggattgactt cggatttgct gacaatcgct cggaatgatt ctgatcagca
    25861 gctgctttca aagcaaatga tcaatacaca agaatatatc gaacaacttg ttaaaccatt
    25921 tcaagagatg gccatcatag atggaaaaga gtttattctc cataacgatg caacattaga
    25981 agtaccaatt gatgaaaaaa aaatccatca agttttggtt attttgctgg ataatgcatt
    26041 aaaatatacg aatccagaag ataagatcat tttggattca tctgttaacc ataaagaatg
    26101 gctgattcac gtaaagaata ctggcccttc tatcagtgaa gaagatcaag caaggatttt
    26161 tgatcgattt tatcgggaag atcaatctcg ggcgaaagag actggcggat acgggcttgg
    26221 cttggcaatt gcaaaacagg taatcgaaca gcatcatgga cagatttctg tatccaatta
    26281 tcttccacaa ggagtcgatt ttaaagtcca attgccgttg aagcctaaaa aataatgctt
    26341 atccctaaaa aagggctgtg acaaaaatct tgtcacagcc cttttttgtt gcgaataaac
    26401 ggtgttccag aagtagcttc ttcggaaata agccgaaatt tctttaagct gaccacttct
    26461 gtcacaacct ctttagctta cgcttacgat tttttattgc ggtcttcaat gtgtacatca
    26521 ccgcttgtgt caatatccaa ttcttctcgg ctaacatctt ctgtcacttt ttcaacatct
    26581 tcatgttctt ctttatgaat cccaacttct tcacgaataa ctgtatgttt gcttacatct
    26641 acttgctctt ctttgattgg gatagtaatc gtgtcatctt ccaatttttc gtctgttccc
    26701 tgtgaagatt gatctgttac tggtttgcgt tcgatgacga tttcttcacg tttgaccggt
    26761 acttctaccg tttcagtatc gttgaccaca tgtttgtgga tgtcgacttc tccagttgtc
    26821 acatcatgtg tatttacatc taatttttct tcttttagtt gaattgtatc atcatctaga
    26881 tttggtttgc gatcgatgtc ttcagtcttg cttgtaccca ttgtatcagt ctcgccaggt
    26941 acgtccatcg gatcagttcc tggtgtcata ttcaatggat ccgttgtatt ttgaaagcct
    27001 gtcgtgccac ctacgtccat tggatctgtt ccagtgccag tttctaatcc agtccccatt
    27061 gtgcttgctg ggctaacagt atcttgtgta tccattgtat cttctgttgt ttctggctga
    27121 cgatagcctt ttactgtgat gacaaatttt ccatcagaaa tatctcctct atattgatat
    27181 aaaggatcat tttctggtgt agccgtctca gaatcatagg tatcaaaaga aaaagtatct
    27241 tttatttttt cccataaaga acggtcttct tcaccatcca tccgattttc atctgtttca
    27301 acatctatac cagacaatcc gtcaaatcct agtctagatg ctgcttcatc agtagtataa
    27361 ataacgatat catctcgttc atacccttcg ttacgcaacc gttcaattac tgataatgct
    27421 tcttccctcg tactatagct tccgatgacc gttaaatttt tctcttccat gtaaattcct
    27481 cctagcaaat agatttatga ttggtatatt gtta
//
LOCUS       DAYYCW010000026        26643 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000026 DAYYCW010000000
VERSION     DAYYCW010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.026"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..227)
                     /locus_tag="ACHAYA_001051"
     CDS             complement(<1..227)
                     /locus_tag="ACHAYA_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8604870.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKI"
     gene            complement(341..433)
                     /locus_tag="ACHAYA_001052"
                     /pseudo
     CDS             complement(341..433)
                     /locus_tag="ACHAYA_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(569..1204)
                     /locus_tag="ACHAYA_001053"
     CDS             complement(569..1204)
                     /locus_tag="ACHAYA_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8604871.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            1716..2858
                     /locus_tag="ACHAYA_001054"
     CDS             1716..2858
                     /locus_tag="ACHAYA_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8604872.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            2851..3057
                     /locus_tag="ACHAYA_001055"
     CDS             2851..3057
                     /locus_tag="ACHAYA_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8604873.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            3136..4329
                     /locus_tag="ACHAYA_001056"
     CDS             3136..4329
                     /locus_tag="ACHAYA_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8604874.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            4534..5673
                     /locus_tag="ACHAYA_001057"
     CDS             4534..5673
                     /locus_tag="ACHAYA_001057"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM8604875.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5666..6466
                     /locus_tag="ACHAYA_001058"
     CDS             5666..6466
                     /locus_tag="ACHAYA_001058"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8604876.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6466..7293
                     /locus_tag="ACHAYA_001059"
     CDS             6466..7293
                     /locus_tag="ACHAYA_001059"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8604877.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7298..8005
                     /locus_tag="ACHAYA_001060"
     CDS             7298..8005
                     /locus_tag="ACHAYA_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8604878.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8105..8971
                     /gene="rfbA"
                     /locus_tag="ACHAYA_001061"
     CDS             8105..8971
                     /gene="rfbA"
                     /locus_tag="ACHAYA_001061"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM8604879.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            8985..9557
                     /gene="rfbC"
                     /locus_tag="ACHAYA_001062"
     CDS             8985..9557
                     /gene="rfbC"
                     /locus_tag="ACHAYA_001062"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM8604880.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9579..10607
                     /gene="rfbB"
                     /locus_tag="ACHAYA_001063"
     CDS             9579..10607
                     /gene="rfbB"
                     /locus_tag="ACHAYA_001063"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM8604881.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10705..11556
                     /gene="rfbD"
                     /locus_tag="ACHAYA_001064"
     CDS             10705..11556
                     /gene="rfbD"
                     /locus_tag="ACHAYA_001064"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM8604882.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11590..13623
                     /locus_tag="ACHAYA_001065"
     CDS             11590..13623
                     /locus_tag="ACHAYA_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604883.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13667..14947
                     /locus_tag="ACHAYA_001066"
     CDS             13667..14947
                     /locus_tag="ACHAYA_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM8604884.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15157..15963
                     /locus_tag="ACHAYA_001067"
     CDS             15157..15963
                     /locus_tag="ACHAYA_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8604885.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            15975..17195
                     /locus_tag="ACHAYA_001068"
     CDS             15975..17195
                     /locus_tag="ACHAYA_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8604886.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17185..18771
                     /locus_tag="ACHAYA_001069"
     CDS             17185..18771
                     /locus_tag="ACHAYA_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8604887.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            18810..20948
                     /locus_tag="ACHAYA_001070"
     CDS             18810..20948
                     /locus_tag="ACHAYA_001070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8604888.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21316..22299)
                     /locus_tag="ACHAYA_001071"
     CDS             complement(21316..22299)
                     /locus_tag="ACHAYA_001071"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8604889.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22677..24068
                     /locus_tag="ACHAYA_001072"
     CDS             22677..24068
                     /locus_tag="ACHAYA_001072"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8604890.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24084..25124
                     /locus_tag="ACHAYA_001073"
     CDS             24084..25124
                     /locus_tag="ACHAYA_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8604891.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25143..26228
                     /locus_tag="ACHAYA_001074"
     CDS             25143..26228
                     /locus_tag="ACHAYA_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8604892.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            26261..>26643
                     /locus_tag="ACHAYA_001075"
     CDS             26261..>26643
                     /locus_tag="ACHAYA_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8604893.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQI"
BASE COUNT         8662 a         4453 c         5414 g         8114 t
ORIGIN      
        1 ctaattttag caaatattac tgcattttca tttccaattc tttgaaaaat tgcttttaaa
       61 atatcttcct ttgaatcgaa atgatgataa atagcacctt tagaaagttt tgtttcattg
      121 ataatatctt gcaatgatgt gttatcatat cctttttcac aaaacatttt tgtagaaaca
      181 tctaaaatga gttttactgt ttcttctgga tatttatttc gtgccataac ttacctccaa
      241 tacaaaccgt tggtctgatt aaagtatatc ttagtatttt atgcttgtca atataaagac
      301 ttgcaatttt ataaaaatgc agaatgcaag cctttttcat ttattctaca atcttccagt
      361 tgctttcctt atgcggtgtc aattcatact gtgatacctg cgttgccttt gcctgattta
      421 ccacaaatca gattttatcc ctttcttcct cttgattgcc cacaacagaa aagaaaaagc
      481 cctgtcaaga gctttgccac cattggtggt gctttgcact cttgatcggg ctttttgttt
      541 gctgtatctt ctcgcaagag gaaaaaagtt actcgtcctc atcatcaaaa ttgttattcc
      601 ttaatacttc tcgtaacagt tccatatctt cttttgagtt cgggtttatc attttcacga
      661 cttgataagg tgtcttatac ttatgtccct ctatgctttc cccaaacata tccatactgt
      721 ataaatcatc atagcggtct aataattttt gaaacttcaa atgctggata aattctttga
      781 ttgggtaaag gtcggacgct tcaatgcttt tcccgttgat atagtctgta atatattccc
      841 gtaacttttc taactcatat tccagccatt cttcctcgct gtcaaagaag ttatcatagt
      901 gtccatgttt cagttctgca tttagacgtt cttcggctct taaaaactga cagacttcct
      961 tttcagattg attgacatac ttccattctc ccgacaacaa ttcattgggc gtaacgtcca
     1021 gcacttccat tatcttttcc agcgtttcat aggcaggata gttcaaccct ctttcaatct
     1081 tggaaaggct ctgcatattg ataccgattt tgtccgcaag ttcctgttgt ttcattcccc
     1141 taaattttct tatggtctgt atattatttc ctaaatggct tattttcatt gcataaccct
     1201 ccatatctcg ttatatcttc gctattataa gtctatcatg cttaatatga tttgtaaagg
     1261 caaaaaaaga tattgacaaa taagcatata agaattacta tgttcttgta atcgaataag
     1321 cacaatagaa tgaaatataa aaacattcaa aaagaaatac ctatctgatt aaatccgtag
     1381 atatgaatat cggttgaaat ggaacttgtt agaaaaggca aaacagaact tgctgacaaa
     1441 gtatttctga cacggtggcg gaagcctctc aggcttaccg ccatgacacc ttgtgtcaag
     1501 gggaagaaat gctttgtcgg ctggcgagcc tgcgagcctt gtctgtgccc ccgttatcta
     1561 acaggggggc actatttcac aaaagacaag tcaaaaacca tacaccccca aacctgtgag
     1621 tagtaatgag aggttttaag aagatagtac atatcacact ttttgaaagg tggtttacag
     1681 gtatttcaag gctattgtgg gattggagga atacaatgaa caatttagaa tttgcacttg
     1741 aaatgaagaa caaacgtctt gccagcggtc tttcacaagg agaacttgca agtcttaccc
     1801 atgtatcaag atatagtatc aaccgctttg agaacggaaa agctaacgcc agcaaggaaa
     1861 cacagaacat tatcctgcgt tgtctgaatt actatatctg cgataagcct ttttatctgc
     1921 tcgttgatta tctgtctgtc cgttttccaa cgactgacgc attggaagtt atccgaaagg
     1981 tgcttggcat gaaagcagat tattttatcc attatgacta tgggtattat ggctataagg
     2041 agcattatgc ttacggggaa atcaaggtca tggcttctga tgatgaacac atgggcgtat
     2101 ttttagaatt aaaaggggca ggctcacgca acatggaata tgtcttacag gcacagaaca
     2161 gggactggta ttcattctta aaccgctgtc ttgactgtgg cggtgttatc cgacgttttg
     2221 acttggcaat caatgatatg tgcggtctgc tggatattcc tgtgctatct gaaaaataca
     2281 gaaatggtgg tgcggaatgt cgctgtaaaa actatgaaaa tgtacaaggt ggaaaattaa
     2341 gcggaaaaaa ccgtaattta gcaagtaccc tttatatcgg ctcaaaggca agcacaaaat
     2401 atttctgcct gtatgaaaaa caaaaggaac aggcaacgaa aaagaaacat accgatatta
     2461 tcaaccgttt tgaaattcgt ctgcgtgaca aaaaggcggt acaggcagtt gaagaactgt
     2521 tattgacgta taaccctcat gggctggtgt tttatctcat taccgatttt gtggaatttc
     2581 ctgactatcc attgtgggag atattcattt cccatgacag tttacctttt gaaatgaacc
     2641 ctgtacctgt caatatggaa cgcactctgc aatggctgga aagacaggtc atgccgtcaa
     2701 tcgtgatgat agaggaaatc gacaggctga caggttcaaa ctacatgaaa atgattgatg
     2761 aatgtaccca cctttctgaa aaacaggaaa tgcttgtgga acagatgtgt acggacatat
     2821 cagacgtaat cgaaagtgag ggggtgtttt atgagtaata cacaggatat tcccgtatgg
     2881 gaaaaataca cccttaccat tgaagaagcg tcaaagtatt tccgcattgg agaaaacaag
     2941 ttaagacgat tggcagagga aaacaaggac gctggctggc tcattatgaa tggcaaccgc
     3001 atacagatta aacgccgaca gtttgaaaag gttattgata aattggacgc aatctaatgc
     3061 aaatgagcct tgtatgtgtt atgatgaaca caagtcatat caaggctctt tccaacaagg
     3121 aaaggagcag acaccatgaa agaaaaaaga cgggataaca aaggtcgtat cctgcatact
     3181 ggagagagcc aacgaacaga cggaaaatac ttatataaat atgtagacgc attgggaaac
     3241 acaaaatatg tgtatgcttg gagattgaca cccacagacc cgacaccaaa gggaaaacgg
     3301 gaaaaaccct cacttcgaga actggaacag cagataagac gggatattga ggacggtatc
     3361 gacagcacag gcaagaaaat gacgctttgc caactctatg ccaaacagaa cgcacagagg
     3421 acaaatgtga agaaaagcac acagaaacaa cgggaacaac tcatgcggtt attaaaagag
     3481 gacaagttag gtgctaggag cattgatacg ataaaaccct ctgacgctaa agaatgggcg
     3541 ttacgcatga aagacaaagg cttttcctac aataccatta acaaccataa acgctcgtta
     3601 aaagcgtcat tctacatagc catacaagat gattatgtga ggaaaaaccc ttttgatttc
     3661 aagctaagtg aagtcataga aaatgatacc aaagagaaag tcgcattgac agaggaacag
     3721 gagcaagcct tattgtcatt tattaagacg gacaacgtgt atcacaagca ttatgatgat
     3781 gtgctgatac tgttaaagac aggacttcgt atctcggaac tgtgcggact gacagtagcc
     3841 gacattgatt tcaagaatga agttgtgatt atcgaccacc agttactaaa gagtaaggaa
     3901 cagggctatt atattgaaac gcctaagacg aaaagcggga taaggcaagt gccattaagc
     3961 aaagaaacga tacaggcatt tcaacgggtt atcaacagcc gaccaaagat aaagtcgata
     4021 gaggtagacg gacacagcgg ttttgtgttt atcaatccga aaggtaatcc aaaagttgcc
     4081 attgattata acgccttatt tatccgtatg gtaaagaaat acaacaagca ccacaaggac
     4141 aatcccttgc cacatattac accgcatacg ctacgccata cattctgcac aaggctggca
     4201 agcaagaaca tgaacccgaa agatttacag tatatcatgg ggcattcaaa catcagtatc
     4261 acaatgaact ggtacgctca tgcgtccata gataccgcaa aatcagaggt tcagcgtcta
     4321 atcgcataga agtatttacc acgattttaa ccacgattga cagggaaaat ataagaagat
     4381 agacctagat atgcgaggtt taccacaaaa gcaaaatgcc cgtagagccg ataaaataag
     4441 gctttgcgga catttgagaa gatataaaaa gatagtcaaa aagacatata taatttataa
     4501 taattatgac tcttaattaa agtaggtgcc catatgcttg tttcttttag tatggttata
     4561 agattttttc tgacaatttt attatcactg atattaacac ctgtattcaa aatcatttca
     4621 gtacaaacag gtatggtcga caaaccaaat gaaagaagaa taaataaagt tccaatgcct
     4681 tcagcgggcg gactgcctat ctttatttca tttgttatat cgactttatt cttattccgt
     4741 aaaattatcc ctcaatcgta tattgtaccg atcttgattg cttctttagt aatcatagtc
     4801 acaggagtgc tcgatgataa atatgagctg acacctaaac aaaaaagtat agggatcttg
     4861 attgcttctc ttattattta ttttgtagca gatatacgta ttgattcctt cacgcttcct
     4921 tttattggat atatccaact aggctggctt agttttcccg taacgatttt ttggatcttc
     4981 ggtatcacga atgcggtcaa tctgattgac ggtttggatg ggctggcggc agggatttca
     5041 ttgatcggtt taattacgat aggaatcatt ggatattttt tcctccatgc ttctacagtg
     5101 tatattccag ttgttatttt ctgtcttgta gcaagtatta tcggtttttt tccttacaat
     5161 ttttatccgg caaagattta tttaggcgat acaggagctc tattcctagg gtttatgatg
     5221 gccgtccttt cactacaagg gctgaagaac gtgacttttg tttcatcgat ttctttgctg
     5281 atcgtcatgg gagtgccggt aacagacacg ttttttgcta tcattaggag aaaagcaaac
     5341 cgtgtttctt tttctacagc agataaaaaa catcttcatc accgactaat cagtttaggc
     5401 tttactcata aaggtgctgt cctgacgatt tatgcgatgg ctttgatgtt ttctttcaca
     5461 gctatggtca tgaattatac tggaagaatc ggaacgatca tattgattat tgccatgttg
     5521 tttgctgcaa ttttattatt tgaattgatt ggtctgatca atgagaaaca tcagtttatt
     5581 ttaaaaacat tgcgattttt agggaataaa gagtaccgta cgcaagtaaa acagaaatat
     5641 ttgaagaaat ggaaccgtca tatagatgaa taacaaatta aaccagccga tccttattag
     5701 tattgtgacg gcaaacagtg ataaaatttt tcggacacta gataatttta tttcaactgt
     5761 aaaagatgaa acagcagtaa aaatccgaat ttttgacaac aattcacttc ccgagtacaa
     5821 aaaaagacta acagagtata ggaaatatcc ctacatcgat atctattttc atgatgaaaa
     5881 ttccggattt ggttatggac ataatcacaa tctgatagct agcgattctc gctatggcat
     5941 tatttgtaac ccagatattt tagtggatga agagacagtt acagcattgg tcttgttatt
     6001 gaaagaacat ccggaatgtg cgatgttagc acctaagatt cttaatgaag atggaacaac
     6061 tcagcacctc atccgtgagc agctggctgt ttttgattac gttttgcgct ttgtgccttt
     6121 caaatgggtc aaaaaaaagt ttgacaaacg actggctgtg tttgaatgcc gaggattgcc
     6181 tactgatgct aatagttatg tacggatgat ctcaggtagt tttatggtag ctgatattga
     6241 taaatttaaa gagataaagg ggtttgatga gcgtttcttc atgtactttg aagacaacga
     6301 cttgtgtttg agttttgaaa aagcaggtta caagttattg tacacaccct tttatgcagt
     6361 tatccatttt tacgggaagg gtgcgcatcg aaatgcaaaa ctgtttcgcg tgtttttgac
     6421 ttctatgatg aaatttttca ataagtgggg gtggcgtttt ttctgatgaa accagaaata
     6481 ttagtagtcg ttgtgcttta tcagcaacta ttcagtcaat ctccagctta tggaccatta
     6541 agcaaagcac ttagcgaaaa gcaaattcag cttattattt acgataatag tcctcaaagg
     6601 cagcaaaatg agctgtttga aaaagaaaat atcgtttatc atcatgatcc gcaaaatcct
     6661 ggtttagcgg tggcttataa ttttgcgttg tctcaagcag atgaaggaac acgttgtctc
     6721 gtcactctgg atcaggatac gaggctagtc gacgattatt ttgaactatt gcgtaaagta
     6781 acctttacag atgaatgtgt tgctgcagtt ccgatgattt tttctgggag ccgacaagtt
     6841 tctcccctat attcagatcg ttatatcaat cgtacagctc aagcagtaga agttgaaggc
     6901 ccaacagctg aacgaatcat ggcaatcaat tcaggtgttg cctggtctgt caaatttcta
     6961 aaaaagattg gcgggtttaa tcctgctttt tctttagatt ttttggatca ttggctgttt
     7021 tggaaagtgg atcagttgga aaaaactgtg gagatccttc ctgtacgaat ggaacatgat
     7081 ctttcggttt tagactatga aaaagtttca agtaatcgat accgttctat tttacaagca
     7141 gaaaatcgat tttatcatga atatgatcaa aggcatttat ccagtcaccg acgacaatta
     7201 ttgttaagaa cagctaaaca gttcttgacc gtcaaaaacc ggcatatctg gcggatgact
     7261 ttgcgctcat atgttaagaa ttggaaggtg tgaattcgtg cgttcggttt gtatagctac
     7321 ctttaatgga ggcagatatt taagagagca gttagatagt attttatctc agctttcagc
     7381 agaagatgaa gtagtcattt ctgatgatgg gtcagtagat gatacatggg atatattaac
     7441 tgaatacgca gaaaaagata agcgaatcca gcttttttcg gggcctcgtc aaggattgat
     7501 cgctaatttt ggttatgcga tccagcagac gaaaggagat ctaatctttt tggcagacca
     7561 agatgatgtt tggttggaga caaaagtgaa aaagatcact gactattttg agcagaatcc
     7621 ggatcagcta gtagtagttt ctgatttaat cattgtggac agcgagctgc aagagatcca
     7681 tgattcgtat ttttcgtttc gaaattccaa agaaggatgg cttaataatg tgataagaag
     7741 tcactatatt ggtgctggta tggctttccg gtcttcttta aaacccgata ttttgccgat
     7801 cccgtcagaa gtgccgatgc atgatatgtg gattggtttg ctgggaggaa agaagactgg
     7861 gttcctaaga gaaccgttaa ctctgtaccg aagacatgat ttaaacgcga gtgagattga
     7921 aacacattct aaattacggc aaaaaatttc ttggcgtgtt catctgatta cggcgttgat
     7981 caaacgaaaa tggtttaaac gttgataaaa gatgtttaaa tccttaatat ttccatgaca
     8041 aatataacaa ttgatgagat aataacaaag tgaaatttga aatcaaaaag gagagcagat
     8101 atccatgaag ggaatcattc ttgctggggg cagcggaaca cgtctgtacc ctttaacaaa
     8161 ggcaacatcg aaacaattga tgccgattta tgacaaacca atgatttatt atccaatgtc
     8221 tactctgatg ttggcgggaa tcaaagaaat tctgattatt tcaacgccac aagatacacc
     8281 acgtttcaag gaattatttg gaaacggaaa tgatttagga ctgcagatcg aatatgcagt
     8341 acaagaaagt ccagatggat tggcacaagc gtttatcatt ggagaagaat ttattggaga
     8401 tgatagtgtc tgcttggtat taggggacaa tatttactat ggtggcggtc tgtctaaaat
     8461 gttgcaacga gcagcttcaa aagatacagg cgcgacagta tttggctatc atgtcaatga
     8521 tccagaacgc tttggtgtag tagaatttga tgagaatatg caagctttgt cgattgaaga
     8581 aaagcctgct cagccaaaat caaattacgc tgtaactggc ctttattttt atgacaatga
     8641 ggtaatttct atcgctaaag gtatcaaacc ttcagaacgt ggagaactag agattacgga
     8701 tgtcaataaa gcatatttgg ataaaggaaa actatccgta gaacttatgg gacgcggatt
     8761 tgcttggctt gatacaggaa cacatgaatc attgcttgaa gcatctacct ttatcgaaac
     8821 aattgaaaaa cgtcagaact taaaagtggc ttgtttggaa gaaattgctt accgtatggg
     8881 ctacatcgat aaggaacaat tgctggccct tgcacagcca ttgaaaaaaa atcaatacgg
     8941 tcaatattta ttgaatttgg ccgctgaata aggagtcctt acagatgaaa gtaacagata
     9001 caaaattaac agatgtcaaa atcgtagaaa tggacgtctt tggcgatcat cgcggtttct
     9061 tcacagaaag ttattcaaaa gcaaaatttg cagaacatgg attgaaatat gactttgttc
     9121 aagacaacca ttcactatca gctgaagctg gtgtccttcg ggggcttcat ttccaaaaag
     9181 gagaagctgc acagacaaaa ttgattcggg tagtcactgg agcagtttta gatgtagtag
     9241 tggatatccg caaaggtagc ccgacttatg gtcaatggga cggatttatc ttatctgaac
     9301 ataatcatcg acagttgctt gtacctaaag gatatgccca tggattcgtt actttgacac
     9361 caaatgtgaa cttcctttat aagtgtgata attactataa tgcagaagca gatgggggca
     9421 tcgcatttga tgatccagat ttagcgattg attggccaat tgatccatca agagcgatta
     9481 tgtcggaaaa agatcaccgt catccgacat taaaagaatt tgaagcagaa aatccatttg
     9541 tttacggaga aatctaagaa ttaaggagaa attttcttat gaaaaacatc attgttaccg
     9601 gaggtgccgg attcatcggc tctaatttcg ttcattacgt ggtaaacaat catccagaag
     9661 tgcatgtcac tgttttggat aaactgactt atgcaggaaa caaagagaac ttggctggac
     9721 ttcctagcga tcgcgtagaa ttggtcgtag gagatgttgc agatgcagaa ttagtggacc
     9781 gtctagtaaa agaagcggac gcagttgttc attacgcggc agaatcccat aacgataatt
     9841 cattaaaaga tcctttccca tttgtacaaa cgaatatcat tggtacgtat acacttttag
     9901 aagcttgccg caagtatgat gtacgctatc atcacgtatc tactgacgag gtttacggcg
     9961 atcttccatt acgtgaagac ctgccaggac atggggaagg cgaaggagaa aaattcactg
    10021 cggaaacacc atataatcct tcaagtccat attcttctac aaaagctggt tcagatctgt
    10081 tagtaaaagc atgggtgcgt tcttttggct tacgtgcaac catttcaaac tgttcaaaca
    10141 actatggtcc ttatcagcat attgaaaaat ttattcctcg ccaagtaacg aatatcttaa
    10201 gcggtattcg tccaaaatta tatggcgaag gaaagaatgt tcgtgactgg atccatacga
    10261 atgatcattc ttcagcagtg tggctgattt tgacgaaagg cagaattggc gaaacctatc
    10321 tgatcggtgc agacggtgaa gaagataata agaccgtgat ggaattgatc ttagaaatga
    10381 tgggtcaacc aaaagatgcg tacgaccatg taaacgaccg tgcaggacac gacttgcgtt
    10441 atgcaatcga ttcgacaaaa ttacgggaag agttaggatg gaagcctgag tttacgaact
    10501 tccgtgatgg gttggctgac acgatcaaat ggtacgaaga acatgaagac tggtggaaaa
    10561 aagaaaaaga ggccgttgaa gcagcatatg ccaaaaatgg acaataatac agagaaaatg
    10621 tcactgtatt aatggaaaaa cacatgtgag gcaggcgctc gcctgtctct tttttaaaat
    10681 aaaaatcaag gagcggataa tcatatgttt ttattaactg gaggaaatgg gcaactaggt
    10741 acagaattgc gccatttatt agatgaaaaa aatctgcaat atgtttcaac agatgcaaaa
    10801 gacttggata tcacagatgc cgagaaaaca atggcttaca tcacagaatt aaaacctgat
    10861 gttattttcc attgtgcagc ttacacagca gtagataaag cagaagaaga agcaaaagaa
    10921 ttagatgaga agatcaatgt tgatggtaca agaaatgtag cacttgcagc aaaagctgtg
    10981 aatgctcaat tcgtctatat cagtacagac tatgttttcg atggaaaaaa caaaaatgta
    11041 gaatacgaag tagacgacca aacgaatcca ctaaatgaat atggtcgtac gaaattatta
    11101 ggcgaaaaag cagtagaaga gattttagaa gactattata tcatacgaac ttcttgggtt
    11161 ttcgggatct atggacacaa tttcgtcttc acgatgcaac gcctagcgga aacccgcgac
    11221 aaactgactg tagtaaatga ccagtttggg cgaccaactt ggacacgtac tttggcagaa
    11281 tttatggtat atgtcatcga agaaaaagca ccatttggta tttatcattt gtcgaacgaa
    11341 aatagctgtt cttggtatga attcgcaaaa gaaatattga aagatacaga tgtagaagtt
    11401 gcacctgtta cgtcagaaga atttccgcaa aaagcaacac gtccgcagta ttcagtcatg
    11461 agcctgaaaa agacagaaga gttaggattt gtcataccaa cttggcaaga agcacttgcg
    11521 caaatgctgg aagctgccaa aaaaatgaat aagtaatttc cttttatcaa atagaatttg
    11581 gagcatgata tggaaaaaca aacaagaagt gcacgacgaa aagaaaagcc aaaagcgaac
    11641 caggcagatt cgaatttggt gcttgacctg tttctaaaac tccgctatct tattggcgtt
    11701 ttagtcatcg tggtcatcgt tacttttaat ttaaacggaa gttcacttgg tgtttgggat
    11761 aaatatgtgt cgcaaagaga cgatgggaaa aaaactgatg tgatttttgg acagaatcgg
    11821 gaagtgcggt cagacgaatg gctggtgcag acaccttttt acctctcaca agctgaagag
    11881 gactttccgt tggtgaacga ggattattca ttaaacggac aaaacatgat cattgcgtat
    11941 aattctcctg taaaggatat tacggttatc ggaaaaccat tcaactgggg attctttttc
    12001 ctcggaagag atcgaggact ttccttttat tgggcgatga aattagtcgt catggtcttg
    12061 cttggattcg aagtactgat gatattaacg aagagaaaaa aagctctatc gctaatcggt
    12121 gcatttgcgc tgacattttc tccagcagtc cagtggtggt tcatgcaaca tgtgggcgat
    12181 ttgattttct ttactttggg actgatgact gctttttatc attatttcta caatcatgat
    12241 aaaaaatggc tacgaacgtt gatgatgctt ttggtggtta ttttcggtat tgggtttatt
    12301 ttagtgattt acccagccca tcaagtgatg ctggcgtact tgctgatttt ttattttgtt
    12361 ggcctgctta tttactatag caagaaagtt tcatgggatc tgttggatgt agtgttgatt
    12421 ggaggggcag ttttatttat tggcggagtc atggttcatt tctggctaac gtcaaaagac
    12481 gcattgttag cttctttgaa taccttatat ccaggaaaac gggtaagtac tggaggaaag
    12541 tggacgattg gcgaattttt ttctttcctt acaaattgga aaattccttt tgaagatatc
    12601 aactattcga ataactctga agtggccctg ttctatcact tttttccaac tgtattccta
    12661 gcaagtccat ttgtgttatt tggcaaaaaa gatagcgaac aaaagttact gggacgtatt
    12721 ttgatgattt tttgtctgtt ttctatcttt tggattaccg ttgggttgcc aaaatggcta
    12781 gcagaaatca ctctgctgtc ttatgttcct ccattaagag ccatccttac ttttagtttt
    12841 gcggcttgtt tattatcgat ctggtttatc gcttatattt ggagagaaga tgttattcct
    12901 atttggtata aattcatttt gttggcagta aatgcatggt cttatttcta tgcgattacg
    12961 cattcgaaaa tggataatta tttttcggat tttgaaacca tcgcagttgc tgtgttggga
    13021 acagtgattt tagcttttgc attatttggc tggagacgct tgttttacct cgcttttgcc
    13081 gccttagtta tcgtatctgg ctttttcgtc aatccagtcg tagaaggtac aggagcgatt
    13141 tttgaaaaaa cactggcaaa agaaatccaa aaaatcgatc aacaagatcc tggtaaagtt
    13201 tggttgtctg aagatgaatt atataacttc accccaacat tgggagtgaa atccttcaat
    13261 acagtacgct tttatccaga tatagctgca tggaagaaaa tcgatccgaa agaagaatac
    13321 gagaagtatt ataatcggta tgctcataca agagctttta tcgctaagga agatacaaag
    13381 ttcattttag atcgtccaga tatgttttcg gtcacgttga attttgaaga cgcagaaaaa
    13441 ttagggatcg actatgtgat cagtaaacga ccattaactg attacaacca attatatgaa
    13501 gcgcagttca cgcagctata tggaccggat aaagacggtt atatgatttt taaagtagaa
    13561 tatccggatt atccagatgt accacaaaca caaactcagg aaaatcagct agtcgtacag
    13621 taataggcta gctgagaata atcctgaaat gttagtagaa aggagagtgg agataattca
    13681 agcaatcgta caaaaatttg tcaacaaaat gagtgtcggt ttcttgcttt tgttactgac
    13741 gctagtcttt tttgtttgga cagcagggtt aggttttcca gcaactttgt ttatatataa
    13801 caatagttca ttacttatcg cttttagtgt ggctattttg ctcattttaa atgtttataa
    13861 agcaaaaggc gtcgattttc tttatctagg tgttgcgctt gcccttttca ttttcttttc
    13921 ttactttcgg gaattaagaa atgagtcttt atttggagac ttactgtttc ctttgattat
    13981 tttattttgt ttagtaatta aatggatcga gtggacaaaa tgggatcgcg gtatttatct
    14041 gattgtcttt tcggtcttcc tcggaatcac gttatatcgt gtatttacag agatccaagt
    14101 accagaagga caaagcatct gggctccagg caattcgctt tctgatattt ggatcaatac
    14161 gaatataatc ggaagttctt tgatgactct tgctttattg atcagtggat ttgccagttc
    14221 ttttgaacga tggtatgtac gaatcatcgg tgcagtagca gttgttgcag cttttctcgg
    14281 gatttgggtc tgccaatcta gaggtgcatt agtcgcgttg attgtttttg cgatattgga
    14341 tcttttacct aaatcgttga tgcgtgtcat tcgtgcgcca tttattgctt acacggttac
    14401 gattctttta gctttgccga tttcttatct ggcggctgtc tctgaaaaag tcaatttgtt
    14461 taccggtcga gaagatattt ggcataaatt ctatcagacg ctaggagaaa aatctgaaca
    14521 aattttacta ggaatgaaaa catttatctt ccaacgagga aaccaatttt taggaaacca
    14581 caatagttat aattctattt tgaatatcta tggattgata ggatttggga ttgctgcttt
    14641 gctgcttatc ctttttattg gtcgcttaac gttgaaagca gatttatcca acgggcagat
    14701 gacttttatc tgggcctttt ttgcggtgat gatgcagtca tttatggagg atacattgac
    14761 ttcataccca tgggttccga ttatttatat cctgttggct atggccgttc atcgatatga
    14821 tgaaccagaa gttgcggagg aattctatgt tatggaagag gcatatgacg atatgactta
    14881 tgatgataca tcttatgagg aacaaccaga tagcgctcat tcttccagga tccaacgcta
    14941 tcattgatag gcacaaaata cgagagaggc tgtgacaacc gtcgcagcct tttttggttt
    15001 attgcaattg tctagcatct tctgtaaaat agaaacgtta gagaaaatct tgtatcatag
    15061 aaaattaaga aagaaataag aacgcagctc ggtaccaaaa atatggtaaa atggcacttg
    15121 actgtaattc actgaaaatc gcttggagga acacaaatgt ttaaagattt gtatttattt
    15181 atgaaggaca tttatcagaa cagaaaatta ttgctacagt tttcgatcaa tgacttcaaa
    15241 tcaagatatg caggatcgtt tttaggaatc ttatgggcgt ttatcaatcc gctatttact
    15301 gtattgattt attggctggt atttggtgtt ggactgaaag cacaaatgat cgatgcaaaa
    15361 tacccattta tcgtatattt agttaccggg atgattccct ggttcttctt ctcagatgca
    15421 tttgcatcaa ctacggttgt ttttcgggaa tatacttatt tagtaaagaa agtcgtattc
    15481 aatattcgta tcttgcccac gacgaaaatc ctatccaatc tgtacaccca cttattcttc
    15541 attctgattg gcatagctgt cgcgcttata aatggtatat atccttcagc aatgtcttta
    15601 cagctgattt attatttatt ttgcatgtgt gctttcttga caggattgac ttggctgacg
    15661 gcatcgatcc aaccattttt acctgatatc atgcaattaa ttacgatcgt gctgcagttg
    15721 atcatgtgga cattaccgat attatggagc cctaatcagt ttgattcagg tcttgtaagc
    15781 atattgaaat taaatccgct gtactatgtt gttcaaggtt atcgtgagtc atttttaagt
    15841 gaagcttggt tctgggagca tggaacatat actttgtatt tctgggtttt cacgttagta
    15901 atgttcgtaa tcggctctgc agtgttccgt agattgaaac cacatttttc agatgtgctg
    15961 taaggagaat agctatgaca gaatatgcaa ttagactaaa agatatcaca aaatcttata
    16021 atatgtacgc aaaaccttcg gatcgatttc gtgaagccct caatcctttt aagaaatcct
    16081 atcatgatgt attttatgcg ttgaaaaacg tgaatgtaga aataaaaaaa ggtgagatga
    16141 tcggtttcgt gggagaaaac ggatcgggaa aatctacaat gttgaaaatc attacgggag
    16201 tactgacacc aacatccggc acgatggaaa tcgaaggaaa aatctcagct ttgctagaac
    16261 taggatcagg attcaatcct gagtattctg gctatgaaaa tatctattta aatggaatgg
    16321 ttctaggatt ttcaagagaa gaagtcgatg aaatggtcga tgatatcatt gaatttgctg
    16381 acatcggaga ccatatttat caacctgtaa aaacttattc aagcgggatg ttcgttcgtc
    16441 tagcttttgc tgtagcaatc aatgtgaatc ccgatatttt gattgtggat gaagcattgg
    16501 cagtaggcga tcttgaattt caactaaaat gtatggaaaa atttacagaa atcaagaact
    16561 cgggaaagac gattttattc gtttctcatg atatcagttc gattcgtcgt ttttgtgatc
    16621 gttcctattg gttgaaaaac ggagaagtcg tcgaatatgg cgatacgatg gaagtaacga
    16681 cgaattatga aaacttttta aagaaaaaat cagttaaaac agtggatcgg aataaaaacc
    16741 aagtagaacg gttcgccgct ccagatatcg tcgacgtgac gaaagcggaa ttgttggata
    16801 aagacaaaaa tccggtggat atgctggaac aaggagaaaa actatacgtt aaagtaactt
    16861 atgaagtaaa agatgataca atcaaaaatc cggttttagg aattgcgcta cgaacagtcg
    16921 acaattttta tgtatgcgga cttaatactc tactagatgg tatcaagatt ccttggaaaa
    16981 aaggagagaa tatcttttat ctggagtatg aaaagttagg tttgttagct ggtgaatatt
    17041 attttgacgt tgcggtcttt gaagaaaatg caacggttcc tttggtatat aagacaaagt
    17101 acatgaactt gtttgtcagc ggttcttata tcggagaagg catcgtggtc ttagaccata
    17161 aatgggaaga agggactcat agcaatgaaa tatgattttg aaatggacct agatgaacaa
    17221 agcagtgtgg gaaaaattgc cgcacagatc aaaccaggaa gtaaagtttt agagtttggt
    17281 cccggaaacg gccgattaac gaaacattta atcggagcaa aacaatgtga agtgagcatt
    17341 gttgagttag ataaggaatt gttcgatttt gtcagcgaat ttgctcaaga tggattttat
    17401 ggtgatatcg aaagttttga gtgggcaaac tattatgctg gacagacatt tgattacgtc
    17461 ttgtttgcgg atgttttaga acatttggta gatcctggaa aaacattgaa gaaagtcaga
    17521 gaatttttga atgaagaagg agagatcctg attacattcc ctaacttggc gcataattct
    17581 gtcatgatcg atttgttcaa taaccaattg ccgtgggctt cttatggtct cctagatgaa
    17641 acccataatt ctttttatac tcatgatggc ttccaaaaag tttttgaaaa agcgggatta
    17701 ttcattaata ttgaagatta tttgtactta gctgtaggtg atacagaatt aaaatcaact
    17761 tatgaagaat tgccggaagc tgtacgctat gatttcaaaa tgcgaccatt tggtgaagtc
    17821 taccaatact ttttctcatt gatgaaacat ccagtagcac aaagttccat tgcagagcca
    17881 caaaattcta attatgtaaa agtgttagaa gtaacccagc aaacgaaaca agatgaaaca
    17941 atccaacaaa taccattcaa taatttcaca ggggaaaatg agacactaag tttccccgta
    18001 tcagaaacaa cagaaagaat ggtgtttcgc ttcgcgcaac aacctagctt tatcgaattc
    18061 agcgcagaag ctgccggaga aaaactaaca tttatcgatt cgaatgctgt agtcaaaaca
    18121 gtaaatgatt gttatttgtt tgatgggaaa gaattaccag agttcgttct aacggatatt
    18181 tctgggaaag aagtgactat ccattgtcac tatcgcttta tcggggaatt gacaccaaca
    18241 atgaaagaat tgttggaaac gattcgtccc atggctgaag tcacaaagca gctttctgag
    18301 aaaaatgacg aattagaaag agaaaatcat catcttcttg aggaaaatac aagactagac
    18361 catacgttaa aaacaaccac aaatcgctat tgcacattgt tggaaagtga tgaatggaca
    18421 ataaaacata gactaggacg acgtaagaaa gaaacttcga aaaaaatcca agaaaaagaa
    18481 ctttctatat gtattgacga gaaaatctgg gatgcagaaa caaaaatatt aaaaatcatc
    18541 ggatggggaa tcgctaattc ggatcgccag cccttgtcct ataagttatc agctgaccaa
    18601 tcgccatttt tcgaagcaat tcctgtttca cgagaagaag tcaatcaagc agaacagctc
    18661 gcaaaaggaa cagaagcggg atttgagtta cgaatccttt gcgagcaaga acgttcattc
    18721 ttagtggaag cagtagctca aaatggccaa tcatggatca ttgaaatgta aataatgatt
    18781 aacttataaa gataaggagt aaacgaatcg tgcaaaaaaa agaaattgcc gtatttatcg
    18841 atcaaatcac tcgtgaacgg gccactggcg atttgcttat tgtgggctgg gcaattgatg
    18901 aagtgaccaa agaaatccct acgataaagg tagaaaaaga aaatgtgatt gctgaagcaa
    18961 cacatgtcgt aagattggat atcaatcatt tatataatct tgatgttaag acccaaagcg
    19021 gttttaaggt acgtttatca gggaaaatga gaggaaaagc catcttagac ttccaaacag
    19081 cgagacatca aaatggcata gctgtgaaat taaacggtaa atacccttat gatgacggca
    19141 ttgaatcttc atgggaaaga aaaaaaagat tactgaaaaa agggataaat tatgcaagga
    19201 ctcatggcgt aaaaaaagcg atccgtcggg tcaaattgga attgaagcca ggatcgatcg
    19261 actatgcaga gtggatcagt cgtcatgaga aaccagattt gaaagagcaa aggaaagaag
    19321 cacaaggatt tgcgtaccgt ccattgattt ccatacttat gcctgtctat aacgttgaaa
    19381 tcaaatggct cgaaaaatgc attgattcgg tactggatca aacgtatgat cattgggaac
    19441 tatgtatcag tgatgatgct tctactgatc cagctatccg aaagtgtctg gaatcctatc
    19501 aagcaaaaga tgatagaatc aaagtcgttt tccgaaaaga aaatggacac atcagtctgg
    19561 caactaattc tgcattggaa atagcagaag gagaatttat agcactctta gataatgatg
    19621 atgaattacc gccatttgca ttatatgaag tggcaaaagt gctaaacgta catccagaac
    19681 tggatgtgat ctacagtgac gaagataaaa tcgatgcaga tggcaatcgc tttgatccgc
    19741 atttcaaagc agactggtcg cctgatacat tgatgggaaa taattatatc tctcacttag
    19801 gcgtatatcg aacaagcatt gttaaagaac ttggcggttt ccgtaaaggc tatgaaggtt
    19861 ctcaagatta tgatttggtc cttcgtgtga cagaacaaat ccctgcagat catatttacc
    19921 acattgaccg agtcttgtat cattggcgga cgatcccagg atcaactgca agtaatggcg
    19981 aagccaaaag ttatatctat gattccggag tgaaagcatt gacagatgcc ctgtctagaa
    20041 gaaacatcaa aggaagcgta cgtcctggca gaatttctgg attttatgag attgcttatg
    20101 acgtcttgca agaggatttg gtcagtgtga tcattccaac gaagaatggc tatgaagatc
    20161 taaaaacttg tgtggattcg atcatcgaaa aaacgtctta ttcaaattat gaaatcatta
    20221 ttgcggataa cggaagtaca gatccaaaaa tgcaagaatt atttgcagaa tacaagcatc
    20281 agctgaaaga ccgctttatc gtagaattga ttgacattcc attcaattat tctcgaatta
    20341 acaatttagc agcagaaaaa gcaaacggca aatacttttt attcttgaat aacgatacag
    20401 aagtgattga gccagattgg atgacggtga tggtttctta tgcacagttt gatcgaatcg
    20461 gctgtgtagg agcaaaactg ttttatccag atgatacaac gcagcatgca ggagtattat
    20521 taggaatcgg tggtgttgca gggcatgcat tgaataacta tgatcgaaca cattgtggtt
    20581 atttcggccg attggtcatt gacgtcaatt acttagcagt cactgcagct tgtatgatgg
    20641 tcaaggcagc tgacttcaat gctgttaacg ggtttgatga aacattagaa gtagcattca
    20701 acgatgttga cttgtgcttg aaagtttatg aactgggtcg ttacaatgtc tatgcacatc
    20761 aagcagaatt gtaccatttc gaatctaaat caagaggtta tgaagatact ccagaaaaac
    20821 aaagacgttt tgctggtgaa atcaaaaaaa tgcaagataa atggccagca tatattgcgc
    20881 atgatccatt ttacaatgat aatttaacaa aagaaggaat cggcgacttt tcgttgagac
    20941 cagattaaac tataataaaa agctgttatg ttgtgattcg ctcgcacata acggcttttt
    21001 atagtttttt gtcaaaaaaa ggcagaaggt gccgactcaa aaaaataagt cgaggttggg
    21061 acagaagtgt ttaactccga gaaataagaa gaaattcacg aaaattgctt ttcaaatttt
    21121 tgtgaatttc ggcttattta agaagaagtt gcttctgctc ccaccgttta ttcgttttta
    21181 aagaatgaga caaaagtgtt tcttactttt gtcccacgct cttattcatg ctcagagcgc
    21241 caatagatac aaaatcatag ccaaagtttc acgaacccta gctgtcaaag aatcgatatt
    21301 ttattttaat ataggctatt ttaaaataaa tggtttaagg tattcctcta caaaagccac
    21361 tctatcaatt atcatgggtt taaaataaga agtgatggag ataaccagat tgttcttagc
    21421 gtcaatataa agaacatttc cactattacc aattgctgaa tatatttcgt tctcagaatc
    21481 taatacccac cataaatagc catatctcat attttgtaag tcttctaaag cactggttct
    21541 aggtttgaac atctcgttta tccacatggg tggtacgatt tgttttcctt tatacatccc
    21601 tttatttagg cataatacac caatttttgc cagatcactt gccgataaac acaaaccata
    21661 tcctgccgtc ccgatattta gtggatcaca aaaccagaca ttggttttag gagacttatc
    21721 cattgtaaac tgcttatgtt cttcagctgt ttcagcataa taattgacat ggtttggtat
    21781 ccctatagga tcaaatagat acttattggc aaaatcaaca gttgttagct tacttgtctg
    21841 atacaacaag ccagttaata tgtgtaaaca gacagtttga tagttgaatt cgccggttat
    21901 accttttctt cctcccaata aatctagtga tgtttttgtc caatcttcac ttgaacagac
    21961 ttttgtccat ggatcgcctt tacatttgta gggtgctttc attgttagca agtgcttgat
    22021 tgtaatagcg taaatagttt tctccccgcg tttcacttta tactctggaa aataatctaa
    22081 tactttatca tcgatacttt tgattaatcc ttgctctaaa gcaatgccaa tcagcaaaga
    22141 tacaatactt ttcgttgctg acatgatatg acaagtatcg tcttttttat aaccattcca
    22201 ttcatctgta taaacttcta ccccatcctt atatgcgaca atttgacata tatttggttg
    22261 tcgaaaacca ataaaatcat gcaactcttg tttattcata agatattacc tcactttctt
    22321 ctataagcat aatgtcatta aaaatcgatt tcaatttttt cgattcttca cgtaatacag
    22381 gtattcttat ggactagaca gcgctttggg aagatggatt ctctgaaaaa tataaaggtt
    22441 cactttatga gtggataaaa aaagcagcaa atacttcgac ttctttaatt taccaacatt
    22501 agtgaaaatc gattcatctg gagatttgta atttagagtc cttgtcttga ttagaggtgt
    22561 ataaagcttt ttattagaca tttttaaatt ccattacaat ttgcttataa actaggtttt
    22621 taaaggtgac tatggtaaga taatagagat attattttgt gattaggagt gtatatatga
    22681 ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg gatgttattg
    22741 tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag atcccattac
    22801 gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt attgcgttga
    22861 acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc gtgtttgtca
    22921 cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt gcgggccgct
    22981 ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg cttttcttaa
    23041 gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat aaaaaggtcg
    23101 tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt tcaaagaaaa
    23161 acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac atcaaaaata
    23221 tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact gaaaaaatca
    23281 aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca acatttgaaa
    23341 acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt attgaagcat
    23401 ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat atcatcgttt
    23461 cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt ttagtcaaag
    23521 tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat caaaaggaat
    23581 tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct ggaccagttt
    23641 tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga gctgtccgct
    23701 ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc gtaggaccac
    23761 gtcctgaacg tcctttcttc gtcgatcagt tcaatgcaga aaacccttat tactacttac
    23821 ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa tatgtctctg
    23881 attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca ttgatgcttg
    23941 atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc tctcaaggac
    24001 ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat gaaaaaatct
    24061 ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc gtaccagtat
    24121 acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg gattatgaag
    24181 agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa atcattcaag
    24241 aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa gggcccggat
    24301 tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc ttcgatgcag
    24361 atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa aagagtccaa
    24421 aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga agaatcgttt
    24481 ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag tttataaaag
    24541 acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa tgctatcgta
    24601 gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt gaggatcgaa
    24661 tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca aaagtagaat
    24721 atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct aacaagttcg
    24781 aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt aataaagacg
    24841 gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt tatagtacct
    24901 ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt gatccagtga
    24961 tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata acaaagttgc
    25021 tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc ttcgttgaag
    25081 tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg gaggaaaata
    25141 taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca gagtctttca
    25201 tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt ttgatttatg
    25261 aagattatgc agatgttcaa gactatcatt cagagattga aagattagga ggccgtattt
    25321 ttgttgtacc taatcctaag aaaaatattt ttaaatattt aatagaagtg aataagttac
    25381 taaaaaaaga atctttttct atagttcaca atcaggttta ttttggcggg ggaattaatt
    25441 tatggttagc caaaaagaat ggaatcagac agagaattgc tcatagccat gcaactgaag
    25501 atggaaaatc tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc aaattgttgt
    25561 tacaaaatgc aacagactac cttgccgtta gtcaagaagc tggtgaaagt ctgtttcaaa
    25621 accacccctt tgagatagtt cataatggta ttgatttaga gctttatagt aaaaattcag
    25681 aagctaaggt taataagaga aaagaattgg atatctcaat gagtactttt gttgtgggaa
    25741 acattggacg attagagaaa caaaaaaatc aatcttattt gttagagatt tttttagaaa
    25801 tactaaaaat gaattctgat tcttacttga ttttagtagg tgaagggtca cttagaaata
    25861 aactgaaagc caaagcagta gaattggata tagagaaaag agtaaaattt ttaggagaaa
    25921 gaaacgatat tcccgagtta ttatctgtga tggatgtttt tgtaatgcct tctctttatg
    25981 aaggtctgcc aatggttgga ttagaagcac aagcagcaaa attaaaatta gtcttagcag
    26041 atactatttc caaagataca aagttaacaa ataatgtgga ttttgtatca ttagatgaaa
    26101 caccacaaga atgggcaaaa aaagtaataa aagagccgta tgctaataaa ataacacaag
    26161 aactaaagag ttatgatgtt tcgtacacta ctgaacaaat ggagagtata tatagtagag
    26221 atcattaaaa atgttcaaga tattttagga gtgattattt ttgaaaatat gttatttgat
    26281 attggcatac aatgatattg gcaacttaaa gagattggtc aatcgattga acgaaaatgc
    26341 agtttttatt attcatatag ataaaaaaac tgatatagaa ccatttattg aagcatttaa
    26401 atcatatgat aatgttgaat ttttatgtaa taaaaaccgt gttaaaattt attggggtgg
    26461 tttttccatt gtacaagcag aaatcaattt agtaaagaga gcattacaaa atgagaaata
    26521 tttaaaatat gtattattat ctggtgctga ttatccaatc aaagataatg agtatattta
    26581 taattatttt aaaaagaata gttcagtgga gtttattcgt ggaatagatc tagatcaaat
    26641 taa
//
LOCUS       DAYYCW010000027        25859 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000027 DAYYCW010000000
VERSION     DAYYCW010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.027"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            208..2268
                     /locus_tag="ACHAYA_001076"
     CDS             208..2268
                     /locus_tag="ACHAYA_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM8604894.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2351..2920
                     /locus_tag="ACHAYA_001077"
     CDS             2351..2920
                     /locus_tag="ACHAYA_001077"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8604895.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3095..3901)
                     /locus_tag="ACHAYA_001078"
     CDS             complement(3095..3901)
                     /locus_tag="ACHAYA_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604896.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4039..5553
                     /locus_tag="ACHAYA_001079"
     CDS             4039..5553
                     /locus_tag="ACHAYA_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM8604897.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5956..6939)
                     /locus_tag="ACHAYA_001080"
     CDS             complement(5956..6939)
                     /locus_tag="ACHAYA_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM8604898.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6944..7591)
                     /locus_tag="ACHAYA_001081"
     CDS             complement(6944..7591)
                     /locus_tag="ACHAYA_001081"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8604899.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7676..8308)
                     /locus_tag="ACHAYA_001082"
     CDS             complement(7676..8308)
                     /locus_tag="ACHAYA_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8604900.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8324..8875)
                     /gene="coaC"
                     /locus_tag="ACHAYA_001083"
     CDS             complement(8324..8875)
                     /gene="coaC"
                     /locus_tag="ACHAYA_001083"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM8604901.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8872..9609)
                     /locus_tag="ACHAYA_001084"
     CDS             complement(8872..9609)
                     /locus_tag="ACHAYA_001084"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM8604902.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9606..10595)
                     /locus_tag="ACHAYA_001085"
     CDS             complement(9606..10595)
                     /locus_tag="ACHAYA_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8604903.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10853..11395
                     /locus_tag="ACHAYA_001086"
     CDS             10853..11395
                     /locus_tag="ACHAYA_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM8604904.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11412..12497
                     /locus_tag="ACHAYA_001087"
     CDS             11412..12497
                     /locus_tag="ACHAYA_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8604905.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12509..13315
                     /locus_tag="ACHAYA_001088"
     CDS             12509..13315
                     /locus_tag="ACHAYA_001088"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8604906.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13316..14143
                     /locus_tag="ACHAYA_001089"
     CDS             13316..14143
                     /locus_tag="ACHAYA_001089"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8604907.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14140..15213
                     /locus_tag="ACHAYA_001090"
     CDS             14140..15213
                     /locus_tag="ACHAYA_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8604908.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15641..18337
                     /locus_tag="ACHAYA_001091"
                     /pseudo
     CDS             15641..18337
                     /locus_tag="ACHAYA_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            18402..18902
                     /locus_tag="ACHAYA_001092"
     CDS             18402..18902
                     /locus_tag="ACHAYA_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8604909.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            18914..19408
                     /locus_tag="ACHAYA_001093"
     CDS             18914..19408
                     /locus_tag="ACHAYA_001093"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8604910.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19429..20238
                     /locus_tag="ACHAYA_001094"
     CDS             19429..20238
                     /locus_tag="ACHAYA_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8604911.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20235..21056
                     /locus_tag="ACHAYA_001095"
     CDS             20235..21056
                     /locus_tag="ACHAYA_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8604912.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21107..23206
                     /locus_tag="ACHAYA_001096"
     CDS             21107..23206
                     /locus_tag="ACHAYA_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM8604913.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23203..24321
                     /locus_tag="ACHAYA_001097"
     CDS             23203..24321
                     /locus_tag="ACHAYA_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8604914.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24360..25238
                     /locus_tag="ACHAYA_001098"
     CDS             24360..25238
                     /locus_tag="ACHAYA_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8604915.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25384..25581
                     /locus_tag="ACHAYA_001099"
     CDS             25384..25581
                     /locus_tag="ACHAYA_001099"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8604916.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            25565..25795
                     /locus_tag="ACHAYA_001100"
     CDS             25565..25795
                     /locus_tag="ACHAYA_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604917.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
BASE COUNT         8189 a         4485 c         5442 g         7743 t
ORIGIN      
        1 ctagatataa ctagatataa ctagatatat tacttaacaa aattaaaaag tacataagtt
       61 attattccga ttttcttgaa acaataaaaa atcaaaaaaa atactctcct aatagtccat
      121 cttcttttga ttttatttat ataatgaaat catcttatga aaattactac ttcatgttta
      181 gacaagagta gggaggacta agaataaatg tgttcaaaaa gacgttcaaa acaactaaat
      241 aaacacgctt gtcgtttaga taaggaacgg tcgtgttctg aaaaacacta cgttaagttt
      301 atgatgcgca cgatgctgat cagtttatct gtaggtgtct atatgcatgt acccgttcag
      361 gcaacgacac aaaccatcaa ggtcgtacgt acctctgttt ctgagaccac tgattcccct
      421 atcactatta ctcatacttt cactgatgca aatggaaaac agctacttcc tgcattgact
      481 tcctataata aagtcagtta tcctccaacg ataaaccact actggttaat tcctaacaaa
      541 acattatgga catcggaaac cggaagcagt gtgccgcttt ctatcttttc cgatatgact
      601 gatcctacaa aaggtgatca gttaaaacag atcactgata ttttgaatca tgctttgaca
      661 gatataaagg acggtggaag cgcacaagtt tcttatgtat atgaaaaaga ttattcacag
      721 tttgaaagaa agaaaatcga tgttctggca gggacaaaag tttcgatatc tgatctggta
      781 gaaaggctga agaacgcaga tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat
      841 gggaatatcg tggatacgac taatacagga acatctacta tttccttgtc ctattttgat
      901 ccaattgctt taaaaacaat gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca
      961 gctatcaaag gaaaagatct tcacttaaga acaggagaga gttacgcccc taaagatctt
     1021 gttgaatatg taacaaaaag cgatggaact tcaggaaata aagaagaaat caaaatgaac
     1081 gggacagtag atactacaac tccgggagaa tatcccgttg tattaactta tatagactcg
     1141 aaaacatcaa atgtggcaga aacaacagct gtagtttatg tagaagcatc tgctgcttca
     1201 tcgactgtca tcactcattt tatagatagt caaacaaata aagaacttca agcagaaaca
     1261 catatgagta gtgtaaatcg tattcctgat ttgcgcctac ctgctgaatt ttctgatcga
     1321 gtgccaaata tagatttatc gacttatgaa caaaatggga aagtcacttc tatttctgat
     1381 tggatgcaag caaacggatt agacagaaaa agtcactggt cggagatcct tgaagtactt
     1441 cactcagaac tgatgaataa taggaagcaa tcaggagggg aaattacctt tacttatgtt
     1501 tatataccag acaatcgttc attagaaggt catgatctta ttgttcatcc aaacgaaaaa
     1561 ataaacaaag aagagttgat ccaatcagca acaaattcct taggtaaacc cgtaccgtta
     1621 aaggatgtat cggttacaat caatgaagta ccattggagg atggttattt gaccaaacaa
     1681 acaggtgatt ttacgattat gtatagttat catgacccgt attcactcca agaactccat
     1741 gctcagtcac tattacatgt tttaccatta aacaaacaga tagatcaaaa agaacaaaaa
     1801 agacaagaag aaagcttgtc agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca
     1861 gggaaagtaa aacaaacaag aagcctagag ttagcaagaa tatctagaaa aatctcagaa
     1921 tctaataaaa cgataaggca agaagctcat ttggccccac aagtttctgg attggaaaat
     1981 gaaattttag agcctaaaat aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg
     2041 gaagatcaga aggaatcaga tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt
     2101 gattccaccg atgagcatct gcagccgcaa gtaccacttc ctagtccggg aacacccgca
     2161 cctggtggag gtattccttc ttctgcttta ggagatttca tgcgaggtat ggcaggaaca
     2221 tgggtatacg ctaatcgaaa tgaatttgac gcagatgact ggttctgatt tatatctcta
     2281 tctgttatag tcaaataagt agctgagtca tatgttattt ggcataatgg agacgaggag
     2341 ggatagaaaa atgttagata aaacgttacc ttacgcagaa atctggatga caagaccact
     2401 tcaacaaaat gttccaatca cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa
     2461 agaagatgaa tctgcttggg cggagattga gacttccgtt ggggaatttc aaacgacaga
     2521 tgaagcactt gagtattttc aacaaaactt tgctccttat cctgaagaac ttgaaagaag
     2581 gatgtttttt gtagagacaa aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa
     2641 aaaatcaaga gagcaagtgt atccggtttt tcattggtta gcagtgaaat cagattacca
     2701 aggaaaaggg ctcgcaaaag cattgactgc cagagtcttg caagaatttc ctaccctcca
     2761 tacggacgga ccaatctatc ttcacacaca gacatggagc catcaggcga ttgctcttta
     2821 taaaaaaatg ggttttactt ttattgctga aaatctagac ggcacaccta atccagatta
     2881 tgaaaaagtt atgcaggttt taagtaaatt agataaataa cgaaatggtg aaataagtcc
     2941 tcagtattgg gataaaaaac gaaatagtca ttcctcatat cttacttttt tgagttaata
     3001 ctatcggata actaatggca tgccatacat tcaaaaaaga aggggcagtg acaaaagtat
     3061 tgtcactgcc ccttctcttg ttttttagca tgttttatcg tttgattttt ttaggaataa
     3121 aattcttgat gaacgtaaat ttttggtaat cgtttcgact atagaggtaa ccaaatacac
     3181 tgaaaacaac tgctccaata aaattaacaa ataaatcctt catagtatcc atgattccca
     3241 cgtctaagta accattttgg atgattgttt tatctgtttt accatctgca ttttgacttt
     3301 caatgattgt ccgagtgata tctggaacat gatagacagt gtttccgtct ttacttaact
     3361 ccacactatt gatttgctgg atgatcgtat ctttttgcat atcgaattgc agtaatttat
     3421 ctgctgaaaa ttccacaaac tcccacataa caccaactgt catcgaaaaa cagaaggaaa
     3481 caactgctag taataaagga gtcaagttca tggtttttgt atttttattg agcaaataaa
     3541 caagggaaaa gccgacaccc ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc
     3601 acattgaaaa atagccataa aaatcactta actctcctaa aactgttgaa ctaaaaataa
     3661 aagataacaa aatgatttct agcatatttg gaatctgtat ctttaatatc ttttccagaa
     3721 aataagggat ttcaaataaa attaatgtca gtaataaaag cattgtattt ccccagtgtt
     3781 ggttccagac ctgatccact agcataataa cgacgatgat acgtaataaa atatagattc
     3841 cgtttgtgat tctacctgca gtttttgtac tgttcgtaaa aaaggttttc aaatgattca
     3901 taaaataact ccttcatgta tatccttaat tatactaaga agcgtgtgtt taccaaagct
     3961 atatgactta aagaaaaaca gagttgatat tgtgaaaaaa atcacttaat atagtataag
     4021 gagaaaaggg ggatacaaat gaaaaaatta gttgctggtt tattaacagc cgttgcactt
     4081 cttgctatga gcgcatgcgg cgcagatcag aaaaatacag cgcagacaag ttctagtacg
     4141 aaggaatctg ccgaagtatc atcaggcgct tctgaacaag tatatacaga tccaaaagaa
     4201 atgaaagaat cttatgatgt cgtgattatt ggagctggtg gtgcagggat ggcagcagct
     4261 atcgaagcaa aagatgctgg gttgaatcca gtaattttgg aaaagatgcc tgttgccgga
     4321 ggaaacacat ctaaatcatc aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa
     4381 gaaggaatca aagactcgaa tgatgccttc tatgaagaaa cgctaaaagg tggtggagga
     4441 acgaatgata aagaaatgct tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg
     4501 ctagatcaaa acggtatcaa actggacaac ttaacgatca ctggagggat gagtgaaaag
     4561 aggacacatc gtccgtctga tggctcggct attggtggat acttagtgga ggggttattg
     4621 aataacgtcc atgagcggga aatccctatt tttgtcaacg cagatgtgac agacattaag
     4681 aaaaatgggg aaacggtcaa tgaagtgact gtacatgttc aaggagaaga accaaaagag
     4741 gtcaccggaa aagcaatcgt agttaccaca ggcggttttg gtgccagcaa agaatttatt
     4801 gaaaaatatc gtcccgattt agctgattat attacgacaa atcaagaagg atcaactgga
     4861 gatggtatca ccatgattga aaaattaggt ggacaaaccg ttgatatgga taaaatacaa
     4921 attcatccaa ctgtgcagca ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa
     4981 ggagcaatct tggtcgatca agaggggaaa agatttgtca atgaaatgga tacaagggat
     5041 aaagtatctg cagcaattac agcattgcct gaaaaatcag cttatttgat ttttgaccaa
     5101 ggcgtgcgtg atcgtgccac agctattgaa ttttatgacc aaaaaggata tgtcacaact
     5161 ggaaagacaa tggaagaatt agcagaaaaa atcgatttac caaaagaaac actagcaaaa
     5221 acagttgatg gatggaatac ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc
     5281 acagccatgg aacatgactt atcgtctccg aattattatg cgatcaaaat cgcaccagga
     5341 atccattaca cgatgggcgg tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa
     5401 aaccagccaa ttagggggtt atatgctgct ggtgaagtca caggtggctt acatggaaac
     5461 aaccggatcg gtggaaattc agttgcggag atcattattt ttgggcgaca agcgggcata
     5521 caagcagctc gatttgcttc ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt
     5581 gaaatcagcg ttttgagcta ataccgaggg tcagctgatt gaacactgtt tagtgaagag
     5641 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caagtaaaaa cagcttctta
     5701 tgtttttaac ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     5761 gaaaaagagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa
     5821 acggcggaag cagaagaaat tccttcggaa ataagctgaa attcacaaaa atttgaaaag
     5881 caattttcgt gaatttcttc ttatttcttg gagttaaacg cttctgtccc agcctctttt
     5941 ttgatcagca tgattttatt cagttatctt caaaatgatt tttccgatgt ttttgtttgc
     6001 ttccatatat tcatgcgctt gtttgactgc gctgaattgg aatgtacggt caatgattgg
     6061 ccgaatcttg ccattttcta gaaaaggccc agctttttgc ataaaagttt gtgcgagctc
     6121 tgctttatac tgatcacttc taggtgtcaa taatgtacca agcagttgga cacgtttccc
     6181 aatcaataca ccaaggtcta tctcaggcac gatggtccca cctagcatac caatcaagac
     6241 ccaacgtcca tcaacggcta tagaagctaa attcttttcc cagtaagaag cgccgatgaa
     6301 atccaaaata acatcgactc cccgaccttc agtgattttt cgtacctctt cttgaaagtc
     6361 ttgtttttta tagttgatca attcatctgc accaagttct ttacaaaagg cgagtttttc
     6421 ttctgatcct gcagtaacga tgattttcgc gtgagaaagc tgtctagcta attggatcgc
     6481 tgctgttccc acaccacttg ctcctgcatg gatcaagaca gtttcgcctt cttgtaattt
     6541 tccaagccag taaagtgttt gataagcagt caaaaaaact tctgagattc cagcagcaga
     6601 aacatagtct agcgtatcag ggagaaggat tgcacgatcg gcaggcatca cagcatattc
     6661 ggcataacca cctagattga ctaatccgta tacttttgtc ccaggagtaa aagtggagat
     6721 atcactttta ttctcaacta cctctccagc tacttcgact cccaaaatag ggtagggagg
     6781 tttcaaagag actgattctc ttttcataat atctgttcga ttgacagctg ctgtatggac
     6841 tttgatcagt aattcgcctt gttttggttt aggaacaggc acttcaattt gttctaatac
     6901 atctacagaa ccaggatgct tgattgaaat ggcacgcatg gatctattcc tcctttgccc
     6961 attcttggaa tgctgtattc aactctttgc taccttcaga aagtactaga gtcgtacgag
     7021 aaatatccgt ttcttccact agagcggcaa gtacagcagc aactgtagag cgagtgatcg
     7081 tttttgatgc cttctcatta ggcttagtta attgaatgga agtaacttct tcatcgttag
     7141 tcaatgtgac tggttgaaga atgacaaaat ctaagttagt tcgtttcagc cattcatctg
     7201 cataatgttt agtaatataa taatctgtca tggactcagg ccattttgct ggttcatctg
     7261 cataaacagc actgaccatc agatagcgag agacatgcgc attttcagct gcgatcactg
     7321 ttttgattgc gccatccaag tctacttgca gaagattctt tccttgagag ccggcagcaa
     7381 aaatcaatac atcgatgcct ttgaatgctt cggtcatttt ttccacggac caagttaaat
     7441 caaaagagac atacgaaaca ttctcttttt ttactaatgt ttgggaagca acatctctca
     7501 cccctgcaaa aatttcatgg gaacttggag ctaaatcttt tatcaggtgt cggccaattt
     7561 gtccgtttgc acctacaaca aaaattttca tttggatcat cctttcgacg tcaaatcttt
     7621 ccattcaata gtaacaccaa aagagaaaaa aatcagagat cggctacgat gactatcagt
     7681 tagtaagaga agggtgcttg aagtatctta acaagacatt gcatactgct gcagtcgcaa
     7741 gacctgcgac gattccttca ggtataccac ttgtaaaaat aaccgctaga atagctgaat
     7801 aaacagcatt taaatttgtt ccgatgactt tggcataatc ttgctggaat aaaaagtaga
     7861 tcatattcat cactaaaatc gtattggtca atgaaccgga aagtccagct aagaataaag
     7921 aaacggaatc tgctttttgt ttcattagtt tgcgcagacc tttataaacg aaataaggaa
     7981 ccaccccaat caagatgcga ggaataaacg cgatcagcaa ggctttccag cttccatgtt
     8041 ctgttccgat gactgggata aacggagaaa agacaaaaga caaaggtgtt tggataactg
     8101 tgctacggat catgctgatc agtccaaatg taccgccaag aaaagcacct agtcttggtc
     8161 ctaaaacgat cgaagcaatg attacaggaa tatgcatggt tgttgcattg attggaccga
     8221 tagggataaa acctaaaggc gtaacagcta ataaaatcaa aatagctaaa aacatcgcag
     8281 ttaatgtaaa gttttttgta tttttcataa gtactccttt tttctaagca tttctttcat
     8341 taagagtcag catgacctgt tcgatgatcg tctgaatatc agctaaggca ccttttccaa
     8401 aatctccaca agctaaaaga gattctcttg gttcaatttc ctgatagccg acagatctca
     8461 atgtttccag attacgctga gtgatcgggt tttgatacat attcgtgttc atggcagggg
     8521 caatcaattt tggtgttccc actggaagcg ccattgcgac tgttgaaaga agatcgtctg
     8581 caatgccgtt tgctagcttg ccgataatgt ttgctgtagc aggcgcgatg agaaacaagt
     8641 cagcttgttt tgctagttcg atatgattga ttacctgcgg atttgattct tgcatgacgt
     8701 ctgtatggac tgcgcgcttc gacagagatt gcaaggttaa tggcgtgata aaatgtgtgc
     8761 tgcttttaga cataatcaca tctacctggg cacctaattt gacgaattga cttgtaagat
     8821 cagcgctttt gtaagcagaa atacttccag taacgcctag taagatcttt ttcattttga
     8881 ttcctccagt ctaattttct cagtaatcaa tgcagcaatc tcagatttac tttgagcttc
     8941 ttccactgtt ccatctttag ataagagata gccgtggtgt gtctcgccat ggatttccgt
     9001 caaatcattt gcgaaaataa aatcagtttt gttatttatc agactatttt ttgcgactgc
     9061 cagaagagat tcctttggta catctactaa tagtttgaaa ccaatgattt ttgcttttgg
     9121 ctgccattca cgtagataag aaatgatttt tggtgttttc ttcaaaacga gcacaagatg
     9181 ctcggtgtta gaagagatct ttgaaacagt ggtatctgtc tgttcatttt ccgcaaacca
     9241 attgtccagt tcttcttgcg ttgaagaagt gggtaggttt tttgccagct gttcttctga
     9301 aaaactaaaa gcaggagtaa aatcactgac tgccatactg tggataatcg catcgtagtg
     9361 ttcttttttc gttagttctt ctaattgtaa aaataagtcc agtgcagatt cgatcgtata
     9421 aatcgttata ttggagtgtc ttttcggttt cagtgcttgt ttcgtagtca cgtaatcgat
     9481 tgtggtatcc ggattggcag ctaaataatc agctaatgct tgccctaatc gtcctgtcga
     9541 atgatttgta atagcacgta cttgatcgat tttttcagtc gttcctccag ctgtgattag
     9601 aatcttcatt tatggtcacc tacatttact agtgttcttc cttggtgtgt tccagcaagt
     9661 agtttagatg ctgtttcagg caattgatcc agagtaatct cttggacgac cagttcatct
     9721 gcaatctgca aatccgccat ctgttgccat aaagaaagac gttgatctat tggtacattg
     9781 acagaatcaa ttccaatcaa ctggatgttt cgcagaataa aaggcaagac agtcgcgttt
     9841 agtttgattc ccccagcatt gccacataaa gctactgcac cgttataaga gatcaatgga
     9901 agaagactgc ttagctgctc gccacctact gtgtcgatga catagtcgat ttgctgtttt
     9961 cctaaagctt ttgttgtctc tggcagaaat tcatcaggtc gtacgacttg caaagcacct
    10021 agacttttta gccatgtgac agactcttct tttctggaaa aggcaatgat tgactggaat
    10081 cctagtttat gtaacagggc tatcgctgta ctgcctacac cgccagaagc accagtaacg
    10141 acaacttttt tatcggaggt cataccttga cggattaaag cgttcacaca caacatagca
    10201 gtaaaccctg ctgtaccaag gatcatcgct tgacgcagcg tcatattttt tggtagagga
    10261 actaaccagt ctcctggtac tttttgatat tggctataac cgcctggatg ggtgacgcca
    10321 agcccaaaac cagtagcaag tacaagatct tcttttgaaa aacgtggatt gttactttgg
    10381 acgacagttc cggataaatc aatccctgga gtcatcggat atgttcgtat cacctgacca
    10441 gactcgctca ttgccaatgc atctttataa ttgacatcag aataggcaat ttttaccaag
    10501 acttcatttt cagccaactc agtaaatggc agatcactga ttgctgtatg gaaaacaggt
    10561 tcctttatta cctgaaaaga tcgaaaagcg ttcattcgtg agacactcct tttaaaattt
    10621 tcttttatca taccataaag cctactcttt tattcttgtt cttccgtaaa tttagacaaa
    10681 aattaggtga aaaataaaga atcaaacaac tagaatttat tccaaaagat aaagaaatat
    10741 tcagcaaaaa aaattttaca aagggttgcc tttttcaata aatttcgtat actaatacga
    10801 gtggtagtaa acaggaagtt tactattaga aaacttcgat tcggaggaaa ttatggaaat
    10861 tggtgagaag ttaagaaatt tgcgcatcca aaagaatttg acacaagaag aactaggcga
    10921 acgcacagat ttgtcaaaag ggtatatttc ccagttggaa cgagatttaa gttcaccatc
    10981 aatggaaaca ttcttttcta ttcttgaagt cttaggtgtg actccagaag aattttttca
    11041 tcaagaagca gcgaatctgc aagtggttta ttcaaaagaa gatcatacgg tttatttcga
    11101 tgaagaaaac gggtatgaat tggaatggct cgtggctgat tcgaatgaaa aagaaatgga
    11161 gcctgtcttg ctgacttttg aaggtgcagg agaatataag acgtttgaac cttcattatc
    11221 tgaaacgttt atttttgtat tagaagggga attagagtta agcttaggag agacggtcta
    11281 tgtggcaaaa gcaggacagt cgatctatta tcaagcgaca aaacaacatc aattaaggaa
    11341 tcatctcaaa aaacgaacaa aagtattgat tgttgcaacg caatcatatt tgtaatatag
    11401 ggggtaaaaa cgtggggaaa ccaatcattt catttgatca agtagtgaaa cgctatgatg
    11461 atgaaacagt attaaaaaaa gtaagttttg agatcgaaca aggaaaattt tatacactgc
    11521 ttggtccttc cggctgtggt aaaacaacga tcctacgtat tatagctgga ttcaccgaag
    11581 ctacagaagg ggacatctat tttgaaggga aacggttgaa tgatttgcca gctaataagc
    11641 gacaagtaaa tacagtcttc caagattatg cactttttcc tcatatgaat gtttttgata
    11701 acgttgcttt tggattgaag atcaagaaaa tgtccaaatc agatatcgaa aagaaggtaa
    11761 aagatgcttt gcgtatggtc cagcttcctg gatacgaaaa acgggagatt agtgaaatgt
    11821 ctggtggtca aagacagcgt gtagcgattg cacgagcaat tgtaaatgaa ccgaaagtat
    11881 tgctattaga tgaaccgtta tcggcgcttg atttgaaact acgtacagat atgcagtatg
    11941 aactgcggga tctgcaacaa cgattaggaa tcacgtttat ttttgttact catgaccaag
    12001 aagaagcgtt ggcaatgagt gatgagattt ttgtcatgaa taaagggaaa atcgtccaaa
    12061 gtggtacacc cgtagatatt tatgatgagc caatcaatca tttcgttgct gattttgttg
    12121 gggagagcaa tatcgtggac ggtgtaatga tcgaagataa tctagttgag tttgttggta
    12181 agcaatttga atgtgtcgat ggcgggatgc gtctaaatga accagttgaa gttgttttac
    12241 gcccagagga tttgacgatc acgacaccag ataaaggaaa actagtcgta acggtcgata
    12301 ctcagctgtt tcgtggtgtc cattatgaaa tcatctgtta tgatgaacag caaaatgaat
    12361 ggatggtcca ttcaacgaaa aaagctaaag aaggaagcaa agtaggattg gcctttgagc
    12421 cagaagatat ccatgtcatg cgtttcaacg agtctgaaga agaattcgat gcacgtcttg
    12481 acagctacga agaatagggg ggacaagaat gaaaacaatg cgcaggatct attccgtccc
    12541 ttatattttg tggctcggtt tattcgtgat cgcgcctgta ttaatgatca tttatcaatc
    12601 tttttttgat atgaatgggc aattcacgct gaataattat ctatcttatt ttacgtcggg
    12661 gacatcgctt tctatgacga tcaattctgt atggtatgca tttttgatca cgtttttcac
    12721 gctgatcatc agttacccga ctgcttattt tttaacaaaa ctgaagcaca agcaattatg
    12781 gctgatgctg attattttgc ctacatgggt gaatctgctg cttaaagctt acgcatttat
    12841 tgggattttc agtgtgcacg gcagcgtgaa tcatttcttt gattttatcg ggatcgggcc
    12901 gaaacagata ttattcacag acttcagttt tctgttcgtg gcaacgtata tcgagattcc
    12961 atttatgatt atgccaattt tcaatgcatt ggaagagatg aatccttctt tgatcagtgc
    13021 tagtagagat ttaggcgcga atagctttga gaccttccga cgagtgattt ttccgttatc
    13081 attaaatggt gtgaaaagtg gtgtccaagc agtatttatt ccttctcttt ctttattcat
    13141 gctgactcgt ctgattggcg gaaatcgggt gatcacttta ggaacggcta tcgaacagca
    13201 tttcttggtt acacaaaact gggggatggg ttctacaatc ggtgtgattt taatcattgc
    13261 tatgttcatc gtgatgctcc tgacaggcga gaagaaaaaa ggaggtcgct cataaatgaa
    13321 aaaatttaaa tggtcgtatc tttatttagg gatcgtcttt ttattcctat atgcaccgat
    13381 cttctatctg attttttatt ccttcaatga aggaggaacg atgaacagct ttacaggatt
    13441 tacttgggaa aattatgaag ctgtttttga agatacacgt ttgatcatta tcgtattgaa
    13501 tacgttcatg ctggctttct tatctgcatt gatcgccacg atcatcggga catttggggc
    13561 tatgggaatc tattacacga aaaaacgacg tgcacggaca gcgttgttga gcatgaataa
    13621 catcttatta gtctctcctg atgttattat cggtgctagt ttcttgattt tctttacact
    13681 actaggattc ggattaggtt ttcagagtgt attgctttcc catattgctt tcagcattcc
    13741 aatcgtcgtc ttgatggtct tgccaaaatt gcaggaaatg aacgattcaa tggtcgatgc
    13801 ggctagagac cttggtgcta ataatttcca agtagtcaaa aatatcattt tgccttatct
    13861 ttctccagga atcatagctg gatattttat ggcatttacc tattctcttg atgattttgc
    13921 ggttacattt ttcgtcacag gaaatggctt tacgacgctc tcagtagaga tctattctcg
    13981 tgcaagacaa ggaatcagtt tagaaatcaa tgcgctaagt gcgttagttt tcttgttctc
    14041 catgatattg gtcattggat actactttat tagtaaagaa aaccgtccaa aacggctgaa
    14101 gaaaaacaga agagtacgcg agggggtggc acggctgcga tgaaaaaatt acaatctctg
    14161 tttttaggta ttctcatcat tattgcggtg ctgctgcttg gtgttcgtca acttgaaaaa
    14221 acaagtggca tgagtggagc aaaggtactt accatctata actggggaga ttatatcgat
    14281 cctagtttga tcacgaaatt tgaaaaagaa tatggataca aagtgaatta tgaaacattt
    14341 gattctaatg aagcgatgtt cacaaaaatc cagcaaggtg ggacaaacta cgacatcgct
    14401 ataccaagtg aatatatgat ccaaaaaatg atcaaagaaa aactagtact tccattagat
    14461 cattcgaaga tcaaaggatt aaataatatt gatccacgat ttttagactt ggattttgat
    14521 ccagataatc agtattcgat tccatatttt tggggaactt tagggatcgt atataacgat
    14581 aaattctttg atgcaaatca aattaagcat tgggatgatt tgtggaagcc ggaattaaaa
    14641 gacagtttga tgctgattga cggtgcacga gaagttatgg ggctttcttt gaatagttta
    14701 ggctattctt tgaatagtaa gaatatggca cagctgacag aagcagcaaa taaattgaat
    14761 catttaacac caaatgtcaa agcaatcgtg gcagatgaga tcaaaatgta tatgatcaat
    14821 gaagaagcct ccgtggcagt gacattttct ggcgaagctg ccgatatgat gtgggaaaat
    14881 gaacatttac attatgtgat cccaccagaa ggatccaatc tttggtttga taatatcgtt
    14941 atgccaaaaa ccgtgaaaaa taaagaaggt gcctatgatt tcatcaactt tatgctagaa
    15001 ccggaaaatg cggcacaaaa tgcagagtat attggttatt ctacaccaaa tgaagaagca
    15061 aaagcgttat tgccaaaaga catttcgtcg gacgaacaat tctatccatc tgatgacacg
    15121 atctcccatc ttgaagtcta caaagatcta ggatcgaaat acctaggcat ctataatgac
    15181 ctattcttag aattcaagat gtataggaga taatggcaaa actcagtaag aatggggact
    15241 tatcaacgtt ctaaagagct aaaaatcgtc ctttttctat ctgagttttc caatcttcta
    15301 ccaaaagcgg atgaaacgcc gctttttttg tgttatttta ttttttctac cacgtttttc
    15361 taccagagaa aagtggacaa aaccattgta aaatagggcc cgagacgggc tagtaaatct
    15421 aagattgaaa aagttacaga aggataaagt aattggaaac tatgcaaata tacttaaccc
    15481 ttctgtaatc tattaataaa actaatatat gaatccagtt caatatttgt agctatgaaa
    15541 aaacataaaa atcatattag ataaaaacta aattggtaga taaattattt tgtcagatat
    15601 ctgaggcaaa tattcgacta tttgtgtttc aaagtaaaaa ttggaataca aatatagaat
    15661 agacatattg cagccgatag tttattatgc ttttagacac attaaactgg aggaaaaaat
    15721 gaaaaagagc gctaaagatc gagtttttga tgagctgata cgactaacag ggaatatgtc
    15781 ttctgtcact gccgagaaaa tttcgaagaa tctaggtatc tctcgtcaga atgccagcca
    15841 ttatttgacg agattagtgg aagataaaaa ggtcgagaaa ttaccgggaa agcctgtttt
    15901 gtggaagcct ttagatgaat atgcggttgt agataatacg gagcaaataa acgaagcctt
    15961 tcattctgta gttggtcatg acggaagtct gagagaggtc attcaaaaat gtatagccgc
    16021 tgtcaaatat ccgcctaacg gactcagtgt attaataaat ggagccacgg gtgttggaaa
    16081 aagtttttta gccacaaaaa tttttgaata cgctatccat gagcaaatta ttgaaaaaga
    16141 tgcaccattt gctatcttaa actgtgccga ttatgcggac aatcccgaac tgctttcggc
    16201 aacactgttt ggatacaaga agggggcctt tacgggggct gaaaaggata ccgatggttt
    16261 attggcaacg gccaacaacg gctatctttt tctcgatgag gtccatcgcc tatcgaaaga
    16321 aaatcaagaa aagctctttc tatttattga tactggaaac tatcgtccag tcggtgaaaa
    16381 tgtaaattgg cattctgcta aggttcgttt tattttcgcc acgacggaaa aaggtgaaaa
    16441 ttatttatta gacacctttg atcggcgtat tcaaattagt gttatgttgc caacttttga
    16501 agatcgcccc attcgagaac gattagagct gattcaatta ttttttcaaa atgaagcgga
    16561 tgttttacaa aaagatatta ttgtttcaag agaagccttg tctttaatgt tatctcatcc
    16621 ctttagtggg aatattggta agctgaagaa tatcattaaa atcagttgtg cggatgtgta
    16681 tagtcgaaca caagaagaac cattaacgat tgggaaaaat gagatcttga ttcagttaaa
    16741 tatgctggaa tccgaagtag aaagcttgcc gattgctagt ttattgataa agaaaaatca
    16801 aaagataaaa acggatcgaa tcaaaccgtt tgattatgat tcgattttgg aaaaaattac
    16861 tcagctgata aatgaaggta atttcaatga aaatttttct tcgataaaga atgaagtaca
    16921 gcatttggtg catcacattg ataaggattc ttttttatca atgagtagaa aattgatgat
    16981 tcagatgttt agaaaggtct gggtatccgt tttaggaaaa acgtacggat tgacgacgag
    17041 tggacctgtt gcagaaatta ttagcagatc ctatgtcatt tccccaagct ataagacaga
    17101 cttaaccggg ataaatcgta cattagctgc aaatctacct cggacgactt acttatgtaa
    17161 tcagttcttg gagcatttac caccgctctc agaaaaggac gagaactttc taagactgct
    17221 tattgtcgtg actttatctg attatgtaga tgaatccatc gagctgaagg gcctgttgct
    17281 tgctcacgga gatagtacgg ctagcagcat tcaagcagta gtgaatcaat tatgcggcaa
    17341 ttatgttttt gaagcgttgg atatgccagt agagagtaat gtgtcagaga ttgtcgaatc
    17401 gacaaggaat ttcgtgaaac ggcaagtgaa tacagatagt ctagtgctga tcgtagatat
    17461 gggctcttta aatcaaattt acacacagat caagaacgat ttaaaagggg agttacttct
    17521 tctgaataat ttgacgactt ccattgcact tgatattggc ttgaagatga cgatgaatgc
    17581 gccatttaaa tagattgcgg aggatgcagc agacaagtat tcaatcaatg ttcaatactt
    17641 tgaaggcttt tctcaaagta gcaacattat catttcctgt atgtcgggtt tggggatttc
    17701 ggacaaattg aaagatgttt ttcgtcatca tttatcggat gaatccatcg aagttttcac
    17761 gaaggactac agagaactac gtgaattaat cgaccaaaat gatgaaactt attttgacaa
    17821 gacaaaattg gtcatcacga cttcggatct tccacgttcc ttttcaattc ctaatattaa
    17881 tgtttatgac atcttagacg gtgatggagc agccaatttg gagcatattt tgagtcctga
    17941 aatgaagccg gcagaattta accgcttgat tcaggagcta gtaaaattct tttcgttgga
    18001 aggaattgcg gaccgtttga actttctgaa cccaacgatc gtgatgagtg aagtggaaac
    18061 agtcatttct aaatatgaaa attactatca tttcacttta aatggccgtg taaaactaaa
    18121 tctctatatg catacggcat tgatgatgga acgtttattc ttggctcgga cagaaaaatc
    18181 tgaagaaccg aattatgatt tgaaaccaga tgaagatgaa ttttattatg tttctagaag
    18241 tatttttcaa tcaatggaga tgaaatataa ttttaaagtc aatgcttatg agctttcgct
    18301 gctctatgaa ttattaggac cgtatattca aaaataaagt gtctaaaaaa gtgtctaatc
    18361 tgtttttttt aggttagaca ctttttttaa tgtgaattaa cgtgattaaa ccaatgtttt
    18421 taaaagttgg cacaggaatt gcttatataa aagtgaacag aaagaaagga attaaccata
    18481 tgaagaaaat tctaatagcg actcacggtc acttagcttc aggctttctt agcagtattc
    18541 aattactgac ggggaaggtt caagagatta cggtgattaa tgcttatgtg gatgagtgtg
    18601 attttgaaca ggagttgaat gtatttgtaa accaggtgaa cgagcaggat cagatttttg
    18661 tttttaccga tttatttggc ggaagtgtca atcagaagat taccaaaacc tttttggaga
    18721 aagagattgc tgtaactatt ttgtctggct ttaatctgcc aattctgctc gaaatagtat
    18781 tggcaaccaa ggaattaaca aaggaggata ttcaggaact ggtaaataaa tgccaacagg
    18841 aattaaagat gaccattctt gatgaggtac atgtagtaaa tgaagaggaa gatttttttt
    18901 aggaggaaga aaaatgatta aagcagtgcg aattgatgaa cgattaattc atggacaagt
    18961 agcgatgact tggacaaaga gtctaaattt aaccgggtta gtagtagcaa gcgatgaggc
    19021 ggctaccaac gatattcaaa agatgacatt gaagatggct gcaccatcga atgtcaaagt
    19081 cattattaaa acagttgatg acgcgattca tttgttaaaa gatccgcggg cgaaggacat
    19141 gcgcttgatg gttttagtcc ctaatgtcaa agatgcggta aaagttgcga aagctttccc
    19201 aacggaaatc gaattaatga acgtggggaa tgctgggaaa atgacaccgc catcggaaga
    19261 taagaaaacc ttatcaaagg aagtgatgtt aacgacgtca gaaattcaag acttaaaaga
    19321 attagttgaa ctttatccag ataccgtttt tcagcctaca ccggcgatgg aaaaacgttc
    19381 agctatctct attttgagtg atttttagaa aaaggaagga gaaaaattat gtatgatgca
    19441 tttgtagtcg ctttggccgt ttttattgga gttgctggac atgaaatgtt cgggatgtcc
    19501 atgctgagtc gtccgattgt tgttgctccg ttagcaggag cgttgatggg ggatttgcag
    19561 acggggttga ttgtcggggc gtcactagaa gccatcttta tgggtgtcgt caatatcgga
    19621 atctctagta cggcggagcc ttctttggct gctgggctgg ctacagcgtt cgcaatcaaa
    19681 atgggaggaa acgtggatgc gattattccc attgtctttc cactagcagt attaggatta
    19741 cagttaatga atatgatttt ttcttttgta tgtggaccga tggctgctaa atttttcaaa
    19801 tttgcaaaat ctggtgatca aaaaggaatc atcaatctgc acttctttat gtggtttgtc
    19861 cactatgcgc tttatgctct tattccattc tttgccgttt tatttggagc agatgttgtc
    19921 aaaagcgtct tggataatat tccagaggtc atcatgaacg gattaacagt cgctggaaat
    19981 ctgctgccgg cagttgggat ggcgatgctg ttacggatgc tttggagtaa aaatattgct
    20041 gtgtggtttt tctttggggc cgttgttatg gcgtatctcg agttaccatt gattgcaatt
    20101 gccgcagttg gaacgattat tgcaattgtt atagcccaaa gagatctaca actaaaaaat
    20161 atatctgttc atgcagttca aagcgatgct gctgtaaatg caacgcttga ccaagaagag
    20221 gaggatttct ttcaatgaaa ttagctgatg gaatttctaa agatgagaaa aaattggttc
    20281 ggaagatgtt ttggcgctcg atgactatgt atgcttcggt gaatccggtg acgatgggcg
    20341 gcggtggttt cgcctattct atgatgccct tcattaatca tttctacaaa agtgaagaag
    20401 atcgagctgc ggcgctggaa cgtcatacac agtatttttc gacgaccatt ccttgcgcgt
    20461 cctttgtcat ggggattgcg gcatcgatgg agaaggaaaa ttctgaaaaa gctggttttg
    20521 acgtggaaag tatcaacgcg atcaaaacga gtttgatggg accattggct ggaattggcg
    20581 attcattgtt ctggggagtt tggcgtgtcg ttaccgcggg actagcgatc aacttagcca
    20641 atgccggaaa tattttggca ccaattatct ttttagttat gttcaatata cctaattact
    20701 tgatgcgata ctatggcggc gttttaggct attcattagg ctctaaatat attgaaaagc
    20761 tgtatgcgaa cgggttaatt gaaattctga caaaagctgc tagtatcttg ggtctgatca
    20821 tggtaggtgc aatgacgatc agtatggtca aattcaatac cgttttgagc ttttcaatga
    20881 agggcgaaga actaatgaat gtccaagaaa tcctcgatag tatttttaaa ggaattgtac
    20941 cgttattggt aacattgagc tgtttcaaat tactagataa aaaagtcggc gtcatcaaaa
    21001 ttatttttgg aatggtcgtc ttgggaattt tattgtcatt acttggaatc gcatagtagg
    21061 aagctgcagc tactaggctg cagttttttt agcaaggagg gaaaagatga ctattaatga
    21121 agtaatttat tcacccaaac acccattttt taatgctgag ttaaacgacg gaaatgtgac
    21181 tacttcaaac gaatacagct actatcggaa tattcctgat gtaaagggat tgaccttcga
    21241 tgtaagtaat caaaagacat tagcaaacct tgatgtttgc gggacgattc aaacgttgac
    21301 cttttatcga gaaaatttac taactgagga aaaaccaggt gtttgggtca ataaacagct
    21361 ctctcaaacc aattcgttat ccttaaaggt tgaagtaaac gggattgtct accatttgtc
    21421 agaatcggat catcgtattg aagtcgatgt gattcaagac tgtttgccta gatatattca
    21481 tcattattcg acgtttaagg tcattgtagt atcctttgct cccatactgg ctaataagcg
    21541 tttaagtatg cttgttcaac aggtttacat tgtgaatgaa acacacgaaa gccttcaagt
    21601 ttcagtgaaa gatgtgcctt cgtatcaaga gaagtattcg gatcagcaaa atgtgttgat
    21661 ttctcaaaaa ggaaataagg acacgatcgc acaacaggaa gtggcaagct tcgccattgc
    21721 ttttgtggac ccaaatgctt ttgaagaagt aatgactttt caggaatctg atatcgaaaa
    21781 atggctgaag gaaacattga actattttgc tcagctattt gggcagctgt caatgccaaa
    21841 cgatagagtt gtccatctct atcgtcgtgc actttatcaa agcttttcta gtttcggtat
    21901 gaatcgacag ggacaggtcg tcggcagcaa ttggggcagc tatcctgcta ctaatcgaat
    21961 ttggaataag gatatgtatt acgcatcatt gccattcacc atgtttgaac cggagttatg
    22021 tcaaaaaacg gttctatggt ttgatcaata tggcgttaag tttcctggtt caaaatttac
    22081 aggaggtatc aatcattctc tgagtaattc gttatccagt gcgttactgg ctagtcttta
    22141 ttttgaacat actggggata caaaattctt tgagcggtgt ccgcaggttc tactgaatgc
    22201 ttcaagaatt attgaggaca tattagctca acgtcggtca ggggaaccaa tgctttttcc
    22261 atctgtttgg ttatcagacg cctttgcact tggaaagtat cataccggca gcaatctttg
    22321 tctttggaag gcctgtattg gacttgcaga aatttttgaa gccctagaac aactgagtct
    22381 tagcaagcga tacaaaaaca ttgcctgtaa actaaaggaa tcaatttgta aacaaatgac
    22441 tatcgatggt ccttttggtg agcaattttt ggagggaatc ggtgatgagg agaaaacaac
    22501 ctattcagtg aagcactatc aaaaaccgat tcttgagcaa gggttaatct ttttaagtga
    22561 tgtgattgtg gatggaaaaa ttaatttatt gatgcatgat ggagaagaat cagacactac
    22621 gttgatgcct ttttatcaat ttttagataa taaagatcat ttatatcaga acaccatggc
    22681 cttttctgct agttctttca atccaaccta tagcgaggaa atcaaaggca tcacttgggg
    22741 attagaatcc ggcgccactt ttcctggctt tatcactgtt ttaatgagcg agctgcacaa
    22801 ccctcaagct ttcgtaaccc gattagaaga actaatttgt ttggcagatt tagacggttc
    22861 gtggtggtgg tggccgtatc gcttagaggc tcaacaagga gaagtcgtgc gagactttgg
    22921 ctgcggcaaa tgcggctggg catcgggtct ttttgtcagc ctaatgatca gtcaatattt
    22981 cggattgaaa ttcaaaaaag gagtattgca aattgatcct gttgataatt taacgttttc
    23041 ttggaagaat ttgaagttcg gtcaagcctt catttcgatt gaatgtacgc aatccgagtt
    23101 gagtatttca aatttgtcag aaaagccatt gaaaataagt gatacgaaaa aaattcttca
    23161 cgtagaaaag ggaaaaacga ttcacattca aaggagaaaa agatgagtat actaaaaaat
    23221 attacccaat ttaaaaaaat ccagagtcga gcaatcactc ccgaaaatcc aacaggcgag
    23281 aaaggtaaag ctgcgatgtt gccaagcgag ctaggaccaa gccgcaaagg tcgtggcgca
    23341 attccgctga cttctggtga agaaactgtg attgcggata ttactggaac gggaataatt
    23401 cgtcatatgt ggatgacgat tcgtgatcga acagaatttg gcagttttgt tttacgtgat
    23461 ttaatcttaa gaatttattg ggatgggtcg gatagccctg ctgttgaaag tcctttaggg
    23521 gatttctttt gtaatgggtt tggagagcgt tgcgaggtga actcgttgcc gatagtggtt
    23581 aatcctacag ggggctttaa ttcctatttc gaaatgcctt tccaaacatc cgctaaaatt
    23641 acgatcgaga atttacatcc gaaggatatg tcctcatttt tttatacgat aaattacgct
    23701 ttggtggacg aattggaaga aaatagccta tattttcacg caaattggca tcgggaacgc
    23761 attaccaaat tacaggagga ttataagtta attgatcgat taaagggtca aggctattac
    23821 gtgggtactt accttgcgct tacctgccta gaacgttatt ggtggggcga aggcgagttt
    23881 aaattctaca ttgatgggga tagtgagtat ccgaccgtaa cctcaacagg ttcagaagat
    23941 tattttggcg gggcgtgggc ctttcatgag tacgatgagt tcggacgtcc acatgcgaaa
    24001 aactattcaa cagccttttt agggtatcct taccaatcaa atagagatgc cacaagagat
    24061 cgttttgaaa cgggaaaatt gaatgccgtt catgcctttg gtgacgacgc aatgccacga
    24121 catggtcttt atcgttggca tcttcaagac cccatctgtt ttaccgaaga tttatcggtc
    24181 actttacaac aaatcggcaa cgatgatgtt cgcttatttg agcggcaaga cgatgttgct
    24241 agtgtcgctt actggtattc aacagaagct tcaggaaata ctaaacgtca tttcactgat
    24301 cgagaacgct tgccgcgtta atgaagtgtt tttcacatat tcgaagggag gaacctatga
    24361 tgatatatgg ggcaattgaa gctggaggga ctaaatttgt ctgtgcagtc ggtactgagg
    24421 aattaacgat tttggaaaga gtcagttttc ccacaacaac tccagttgaa acaatgaagc
    24481 aggtcattga tttttttaaa ccttttaagg aacaattggc aggcatcggg gtcggctgct
    24541 ttggtccaat tgatattcaa tgcaaatcaa cgagtttcgg tcatatcaca tcaacaccaa
    24601 aacttgcttg gcaaaatttc aattttgtag gaacgctaaa gcaatatttc aatattccga
    24661 ttgaatggac aacagatgtc aatgcggctt gctatggtga atatgttgct ggttctggca
    24721 aagggttagc aggtgttgca tacttcactg tgggaacagg tatcggaggt ggtgccttgt
    24781 tcaatgggac tttcgtggaa gggttcagtc atccggaaat ggggcatatg ttgatcaagc
    24841 gacatccaaa ggacaatttt tcaggaaact gtcccttcca ccaagattgt ttagagggga
    24901 tggcagcagg accagcgatt gaaaaacggt tgggtgtaaa aggccagaat ttattggctg
    24961 acgattcgtt ttggcagatt gaagcctttt accttgccca atgtgcatac aatacaacac
    25021 tgatgttttc gccagataga attatttttg gcggtggtgt aatgaaacaa gaacacatga
    25081 agaaaaaagt tcaggataaa tttgttgagc ttataaatgg atatgttgaa ataccaccaa
    25141 ttgatagcta cattattacg cccgaattag gtgataatgc cggaataatc ggcggattag
    25201 cattggccag aaaagcggta agaaataagc aaccataatt aatgaatgtt tcattgatac
    25261 tcatgtcaaa agtatttaac cgagtaatca aatgatcaaa tgaagttgga taaagcgaaa
    25321 atgaattcaa ataaaaaggc tctaagtcaa atcgtgttgt tttcattaaa tcactgaaag
    25381 gaaatgcttc tgcgtttcct tttttctttt tgttgtgcgc tgtatacctt gaggatattc
    25441 actggtggcg tacacttaaa tagtaatata ttgtgttact ttatgtctgt ggtaatagtt
    25501 atagttgctt accaatgtat gttcattttt cgaaatgttg acttcataat ccttggctta
    25561 atgtttgaat ttttttggtg atagattttt tcagccgtat aaaataaatc gtaattctat
    25621 tatagagagt tgtgattata aaaagatagc aagaaaaaaa aagaaagttt ctatacattt
    25681 taaatatttt attcctattt ctattgctaa aaaagtagaa attctatatt tacctttaac
    25741 tctcgcttct cttgcaaatg tagttagcat tttaatcgat aataaactca ggtagttata
    25801 ttaccttgaa atgattgata attttgtcac ttctattttt tttgcaataa attttttat
//
LOCUS       DAYYCW010000028        25755 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000028 DAYYCW010000000
VERSION     DAYYCW010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.028"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            218..772
                     /locus_tag="ACHAYA_001101"
     CDS             218..772
                     /locus_tag="ACHAYA_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8604918.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(945..1418)
                     /locus_tag="ACHAYA_001102"
     CDS             complement(945..1418)
                     /locus_tag="ACHAYA_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8604919.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1430..2377
                     /locus_tag="ACHAYA_001103"
                     /pseudo
     CDS             1430..2377
                     /locus_tag="ACHAYA_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            2648..3083
                     /locus_tag="ACHAYA_001104"
                     /pseudo
     CDS             2648..3083
                     /locus_tag="ACHAYA_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            3099..3932
                     /locus_tag="ACHAYA_001105"
     CDS             3099..3932
                     /locus_tag="ACHAYA_001105"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8604920.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            4177..5202
                     /locus_tag="ACHAYA_001106"
     CDS             4177..5202
                     /locus_tag="ACHAYA_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8604921.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5340..5465
                     /locus_tag="ACHAYA_001107"
     CDS             5340..5465
                     /locus_tag="ACHAYA_001107"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604922.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            5490..6605
                     /locus_tag="ACHAYA_001108"
                     /pseudo
     CDS             5490..6605
                     /locus_tag="ACHAYA_001108"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            6688..7584
                     /locus_tag="ACHAYA_001109"
                     /pseudo
     CDS             6688..7584
                     /locus_tag="ACHAYA_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            7600..8424
                     /locus_tag="ACHAYA_001110"
     CDS             7600..8424
                     /locus_tag="ACHAYA_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8604923.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8456..9766
                     /locus_tag="ACHAYA_001111"
     CDS             8456..9766
                     /locus_tag="ACHAYA_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFM8604924.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9914..11173
                     /locus_tag="ACHAYA_001112"
     CDS             9914..11173
                     /locus_tag="ACHAYA_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8604925.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11183..12055
                     /locus_tag="ACHAYA_001113"
     CDS             11183..12055
                     /locus_tag="ACHAYA_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8604926.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12067..12897
                     /locus_tag="ACHAYA_001114"
     CDS             12067..12897
                     /locus_tag="ACHAYA_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8604927.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12937..15120
                     /locus_tag="ACHAYA_001115"
     CDS             12937..15120
                     /locus_tag="ACHAYA_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8604928.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15181..16401
                     /locus_tag="ACHAYA_001116"
     CDS             15181..16401
                     /locus_tag="ACHAYA_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8604929.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16398..17858
                     /gene="gtfA"
                     /locus_tag="ACHAYA_001117"
     CDS             16398..17858
                     /gene="gtfA"
                     /locus_tag="ACHAYA_001117"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFM8604930.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17935..19041
                     /locus_tag="ACHAYA_001118"
                     /pseudo
     CDS             17935..19041
                     /locus_tag="ACHAYA_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19363..19572
                     /locus_tag="ACHAYA_001119"
     CDS             19363..19572
                     /locus_tag="ACHAYA_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8604931.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19565..19714
                     /locus_tag="ACHAYA_001120"
     CDS             19565..19714
                     /locus_tag="ACHAYA_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFM8604932.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19868..20533
                     /locus_tag="ACHAYA_001121"
     CDS             19868..20533
                     /locus_tag="ACHAYA_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8604933.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20817..21902)
                     /gene="xerS"
                     /locus_tag="ACHAYA_001122"
     CDS             complement(20817..21902)
                     /gene="xerS"
                     /locus_tag="ACHAYA_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM8604934.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22236..22469
                     /locus_tag="ACHAYA_001123"
     CDS             22236..22469
                     /locus_tag="ACHAYA_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFM8604935.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22466..22873
                     /locus_tag="ACHAYA_001124"
     CDS             22466..22873
                     /locus_tag="ACHAYA_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8604936.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24010..24528)
                     /locus_tag="ACHAYA_001125"
     CDS             complement(24010..24528)
                     /locus_tag="ACHAYA_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM8604937.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            24895..25143
                     /locus_tag="ACHAYA_001126"
     CDS             24895..25143
                     /locus_tag="ACHAYA_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604938.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            25646..>25755
                     /locus_tag="ACHAYA_001127"
     CDS             25646..>25755
                     /locus_tag="ACHAYA_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8604939.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         8784 a         3933 c         4800 g         8238 t
ORIGIN      
        1 ctaaaattta aataagagct tagaatcttc atataagtaa ggttaatttt tttgtacaaa
       61 aaagtcggaa tattctgagt tactgaaatg atataaaatc ataaactaaa tcccttgatt
      121 ttattgactt tcaaaagttt atatatataa gttaatgatt atggtaaaat atatacataa
      181 tcattaactc agaatatttt taagaaggga aacaatagtg agctataatg tacaaccgtt
      241 aaggacaaat gaagagatac aagattttca gttttggcta agaagaacaa gtaatccgga
      301 aagagatagt tttttatttt tgtttggaat caataatggg ttacgtatgt cagatattat
      361 aggattaaaa gttggagata tacgaggcaa aagtaagcct attattgttg aacataaaac
      421 aggtaagcga aaaccgattt ttatagataa tttgagagaa gaaattttat tatatactga
      481 aggtaaagaa gaaaatgatt ggttatttcc gagtcggcaa caaggtcgac atataacaag
      541 ggatcgtgtc tatcagatat atgcagatat agcagaaaaa ttaggacgag atgatatagg
      601 aacacataca ttaagaaaaa cttttgggta tcactactac aagaaaacta gagacattgc
      661 taccttaatg tttattttta atcatagctc gcaagcaatt acaaaaaggt acattggtat
      721 aactgaagat gaaataggag catcacttcg tggatttaaa ctaggtgtat aatttaaaaa
      781 tttttataaa cagtaaagac aaagattaaa agctattttt atctagaacg attttatttc
      841 ataaagttcg cggaataaaa gctggggtta ctggtacttg gtataggttc gtgttatata
      901 aactaagtaa ttaaataaca tttttaggtt ggaaaaggat gaaattaaaa aaagagaatc
      961 aatacggtct caaaaacgca aacgctgcgg aataagaaaa atactgataa ctcaacgttt
     1021 agcagactat ctaataggga tagtctgctt ttttcgtttt tgaccatact tttgaccttc
     1081 tgcaaacttc cttgcttttc ctctgtagaa ctcatgaaat ctgcaaaacg aattgccgtt
     1141 tcctctgtct gctttttcgc gatatgggtg tatatatcat ccatagtggt tttaatatct
     1201 gtatgtccaa gtcgaacata cacttctttg acagacgcac cagattcaaa gagcagacta
     1261 caatgggtat gacgaaaacc atgaagtgtg atatgtttta agttattctc tttaatgatg
     1321 attttcaaat tgtgatttac atagtctaaa taaaggggct tattttcttc tgatgaaaac
     1381 aaaagttgtt caccatcttt tataggtttg attctcattt ttagatatta tgcccgcacg
     1441 tattcatcac atttctattg tgaatcgctt tattcgaccg acatttgact tttatcataa
     1501 tatactcggt ttaaaattac taatgaaaac aattaatcaa gacgatcata ccatgtatca
     1561 cctgtttttt tcagataatc accaccgtgt gggaactgaa ctgacttttt ttgaagtaca
     1621 agaagggaac aaccagtttt ttggaaccaa tacgattgaa cgaacgatac taaaagttcc
     1681 ttccgaagca tcattgcatt tttgggaaat atattttgaa acacaaggcg tttgccatta
     1741 tggaatcgaa tcattttctg gacgacctat cttacgtttt gaagggccag atgcaacacg
     1801 attagcttta gttcccttac gcgaatttga agatattgat gattatttcc cgtatgtcac
     1861 agaccagatc cctacagagc atgccatttt aggaattgat gccattcaac tacgcgtaca
     1921 atattccgat gcgactgaac gagagttatt gtcggtaggc tggtagcaca aagaatacgt
     1981 cagtttcttt tcgggtcatc aacgagtgac aatcttagaa aatcaagaca ctcagttcta
     2041 tcaagaagtt cagattattc aagatcgtga gaatccagtt gccattgaag ggattggtag
     2101 cgtccatcat gttgcgtttg gcgtagaaaa taaatctgat ttacaacgca tcgacaaaca
     2161 attacaagag cgcaatttta taaattcagg aataaaagat cgtgaatttt ttgtatcact
     2221 ttattatcgt gacccaaatc aattattgat tgaaattgct acaggggaag gaaacctaga
     2281 tgcaaaagcg tatgaaaatc aaagtccacg ctttgaagaa attcctttat ttttaccgca
     2341 gtacttaaat ttcattcgtg aacaagtgga gcaataatta gctaatcaaa tccatgacga
     2401 agagtaagag gttgactcat aagtcaaaaa ataaagaaaa acgcatcaaa tccattctaa
     2461 agaatgttga tttgatgcgg ttcctatttt tataaactat cattttcgac taataaaact
     2521 acttatgagt cagcctctct ttttttcttg atttttatac ctctttctag gtaaactaaa
     2581 catctgccaa aaataccgtg tctcattgaa acattctcaa ctatttcaaa ataaagggga
     2641 gtaccaaatg aacgagtata ttgaaattat ccacgcaaga gaaacaactt aaaaatgtca
     2701 gtctcaaaat tcccaaaaat aaaattagta tttttaccag cgtatctggt tcaggaaaat
     2761 catcgattgt ttttgatact attacccaag aagctggtcg tcaactgaat gacacctatg
     2821 atagttttac ccgtttattt ttacctaaat acagtcgtcc agaggttgat gagattgata
     2881 atctttctac ggcgattgtg gtcaatcaaa agaagattgg tggcagtgcg cgctcaacgt
     2941 tagggacgat tactaacatc aatgcgttat ttcgtgtgtt gttttcatgt tttgcaacac
     3001 ctagtttagg atatgcaaat gcttattcat ttaatgatcc tagtggaatg tgttctgtct
     3061 gtcagggaat cggtaagacg attgcatttt taataggatt gctttatttt tcggctattg
     3121 ataatgctga ttttgtattt tctgatccat ttaccgcaga agttttgtct ggaattgagg
     3181 aacaggcaaa gaatcatggt tattttgtat tggtgcataa tgttaattca gcagaagatg
     3241 ttaaaactat tcagagaaat tggcgttttg aggggttcat tgttgttggt gtacgtgtaa
     3301 aagaattttt ggaattaaat aacgtttttg atggtcctgt tgtttatatt gatacgcatc
     3361 tttcaaaaga gacattaaga gaacttgaac aacaaatgaa tcgtttattt gtaaatacaa
     3421 atgattacca agctagtaca ttggcagtgg aacatttgat tcattatggt catgagaaaa
     3481 tagcgtttct ttcttttgac tacattgtag gtgaaccaag tgttattcaa gaacgacatc
     3541 aagcttatat cgatacatta aagaaatatc atttaacctt taaatcgcat tggatatata
     3601 acaactctga atttgagcga atttatcaag aattagctga atttactgca attgttgtta
     3661 ctggagatta tttagcggct aaattagtaa aatttttaaa aaataagaat aaacgagtga
     3721 tggaacaatt atcgattatt ggatttgatg atattacttt tgcagaatta atggatcctg
     3781 cgttgacaac aatacgacta aatccaggta acaagggggc gattgctttt caccaactga
     3841 ttggactggc caaacaagaa gaaataaaaa atcaagtact actattagag ggagaattga
     3901 tttctcgaga ctctgttcac aaaatcaaat aaaaaagctc tgtgaaataa gtatagttaa
     3961 caataaaata tccgaacgat agagagaatg aactgtgatc tagcagttcc tcgtcgcaaa
     4021 ttcactagaa ttagcattat ttactctaaa ttgttcggat attttttctt gtttttaatt
     4081 atgattacag tgcctatttt taacgaaatt tgcttatttt caaaatgtat gattatatgt
     4141 tagtaaacac tttcaaatat gaaaagagga gctattatgg taggaattag ggatgttgca
     4201 aaacgggcgg gtgtgtccat tgcaacagta tcgcgcgtgc taaatgagga tccgacactt
     4261 tcagtaaccg atgaaaccaa acgaaagatt gcagaatcgg ttaattttta tcattatgtc
     4321 aaaaaaagtc ccgttgcaaa aaatttaaca aatattgcat taataacgac agtttctgaa
     4381 gtagatgagt tggaagatcc ctattttcga acgattcgca gagggattca agtcgaagca
     4441 gaacaaagca aagtcaattt gaagaaaatt attcgtttgt cagaaacacc gttggagcaa
     4501 gacgatttgc taaaatgtca aggtgtgttg attattgggc aggtgctatc ttctgtgatt
     4561 gaagaagtat cagcaattaa ttcgaatgtc gtagtgattg atgaccctag tattgaagct
     4621 gaagtagatg cagtttatac tgatttaaca aaagcaacga atcagcattt agatcgattg
     4681 tataaaaaag gacaccgaaa cattgcgttt atcggaggag gacgtgttcg tttagatagc
     4741 caaggaaata aacacgtgtc agatgatgat tatcgaaaaa tagcctatga gacgtggatg
     4801 aaacaaaaag gattagaaaa atatagtcaa atttatttag aaggctggac cacacttgac
     4861 ggaatgcagg cagcagattt acttttgaaa aaacatgcta aagataagtt gccgactgct
     4921 atcatggtag gaaatgatcc tattgctgtg ggggtttatc gttcacttca aaaaaatggt
     4981 ctacatattc cagaagatat ttcgattgtg agttttgata atattgaagt tgctgaattt
     5041 ttaacacctg cattaaatac ggtgaatgtg aatacagaag aaattggaaa actagcagtt
     5101 cgcatggtta atgaacgaat aaaagaagtt agaaatgttt cgatcaaagt gattgtttct
     5161 aataatatta ttgttagaga aagtgaaaaa aatatcgatt aaaaaagtaa acatccaaaa
     5221 aagggtgttt acttttttgt ttactaatat ttttagtaaa aaatctcgac aaaacattaa
     5281 gtaaacagtt atggtgttgt taacaaaaag aaaacgcatt caaaacagga ggaattaaga
     5341 tgaaacattt caaaaaaata gctttaacag ggatagctgt tttaactggg acattactcg
     5401 cagcatgtgg tgggggagat agtaattcta aagacgggca agtaacttta agctatgcga
     5461 gttgagatac tgaccaagct ccaggactaa gaaaaatgtt agatgaattt gaaaaacaaa
     5521 atcctgatat caaagtagaa atggagacaa cgccttggga tcaatattgg gttaaattag
     5581 aagctgcaac aacgggaggt aatatgccag atgtagtaac catgcactct agcgaatcct
     5641 ataaatatat gtcacagaat gctttgatgg gacttgatga tgttatcaaa gaaaatgata
     5701 ttgacatggg aaactttact gaaggaattg ctgatttcta tacgcttgaa gacgaactat
     5761 acgcaatccc taaagatgct tctgtagtgg ggctatggta caacaaagaa ttatttgata
     5821 atgcaggtgt cgattatcca gatgaaacat ggacatggga aacactcaga caagctgcaa
     5881 ttgatttaac agatgaatca aaaggcatct atggttttgc atcacatatt ggaacagaga
     5941 ctggctactg gccatttatt tatcaaaatg ggggagaagt atttgccgat gacaatacaa
     6001 aatcaggttt gaatacccca gaagcacaag aagctttagc tttttatcga gatattgcac
     6061 ttgttgatca tgcttcacca acaattacag aattacaaga tcctgataag ttaagtcgtt
     6121 ttcaatcagg tagattagca atgatgattg aaggaaactg gtttgcaccg ggatttttga
     6181 aaaatgacta tctagttgaa aatgttgctt ctgccgtgtt acctaaggga aaagttaatg
     6241 caacaattac aaatggactt ggttgggctg ccagtgcgag tacaaaatac ccagaagaag
     6301 tgaaaaaact aatggcattt ttagctagtg aggacgccaa taaaatccaa gcggaaacgg
     6361 gggcatcaat cccagcacta aaaggctttg aagacgagtg ggctgcttct tcagatacaa
     6421 ttgatttgag tgcttttgga aaaatgttgg agtatggagt ccctcgtcca tacaataaag
     6481 atggattacg agctgaacaa gcagaagttc aaattattaa ccgtattctg tctggtgaag
     6541 taactgttga agatggaacc gctcaaattg aaaaagaagt caatgcgata ttgagcggaa
     6601 attaaattag ttaattatac aatatacagg cgaaaggatt ctatttcgtc tgtatattaa
     6661 tggatgaata ccaaaaaggg ggatattatg aaaaatcgaa tttttggaaa atatcgtaaa
     6721 gatcaagtga tatggggcta tatttttatt tttccaacgg cattaggatt aattttatta
     6781 aatatatggc cagcaattca aacagcaatt ttaagttttc aaaaaacggt agggttcggt
     6841 gatactgaat gggtcggatt aagaaattac caaaaaatgt ttgcagacgg tgaagttttt
     6901 cagtctttat taaacacatt aatttatgca gtggtttcga tacctagtat tgttgtatta
     6961 tccttgttgg tggcagtttt aatgaataga aaagttaaag gattgtcgat ttatcggaca
     7021 atttattttc taccagtcgt tgctgcacca tccgcagtcg caatgatttg gcgctggatg
     7081 ttcaataatg attatggact gattaataac atgttggcta aaataggtct cgatggacca
     7141 gcatggttaa cggatccatc gattgcgatt tattctatta tcattgtagg agtttggagt
     7201 gctattggtt ataacatggt gttattacta gccggcttat aagaaattcc taaagattat
     7261 tatgaagcag cggaaattga tggtgctagt ccaatccagc agtttttcaa cattacgtta
     7321 ccattagtta caccaacatt attttttgta gttgtaacaa cagttatcaa tgctttccaa
     7381 gtatttgatg ttatttttat gatgattact cctaatagta ccgccatgtt taaatcacaa
     7441 tcattagcct atttattcta taaacattca tttattttaa atgacaaggg atatggttcg
     7501 gcaatcgtta tgttcctttt agtgattata ttaattatta ctgcaattca aatgaagttg
     7561 cagaaaaagt gggtaaacta ttaaggaggc tatcaaatta tgaaaaataa atttttttca
     7621 aatgcgccaa tccatatcat gcttatttta ggtggtattg caatggcatt accttttatt
     7681 tggatgattt taacttcagg gaaaacgttg gttgaatcta cccaaattcc acctacaatt
     7741 ttccctgaga aatttcagct aagtaactat caacaagtac ttgatttatt accattcatg
     7801 gctttttata tcaatacatt tttaatggtt gttggacgag tgattggctc ggttttattt
     7861 tcagcaatgg ctgcatatgc ttgtgcgcgt ctaagttttc cagggaaaaa tatccttttc
     7921 agcttagtac ttattcaaat gatgattccc ggttccattt ttattactcc acagtattta
     7981 ttagcacaaa aattgggtat tttaaataca attccagcat taatattacc aggggtagtt
     8041 agtgcgtttg gtacgttttt attaagacaa tttttcttag gtttacctga tgaattggag
     8101 gaagcggcct atttagaagg tgccaatatt tggacgatct ttttcaaaat tatgttgccg
     8161 cttgctaaat caggattggt tgcacttgga atattcacag cgttgtttgc atttaaagat
     8221 ttaatgtggc cattgattgt caatacttca attgataaga tgcctttagc tccgggatta
     8281 gcaaacttac aaggacaata tgccacgaac ttcccacaat taatggcagg ttcgttatta
     8341 gcaatttggc caatagttgt gatattcatc gttttccaaa gaaaattcat tgaaggaatt
     8401 gctacaacag gtagtaaatt ataaaactat taattagtga ttggagagta agtacatgac
     8461 aaaaattaca tttattggtg caggaagtac tatttttgca aaaaacgtat taggcgatgc
     8521 gatgttaact ccaagtctac aagatgcaca tattgcattg tacgatattg atgaaaaacg
     8581 actaaaagaa tcaaatttaa tgcttaaaaa tattaataaa aattcgaatg aaaatcgtgc
     8641 tgatattgtt tcttattatg atcgcaaaga agcgttaaga gaggctgatt ttgttatcaa
     8701 tgctattcaa gtgggtggtt ataaaccttc aacagtgatt gattttgata ttcctaaaaa
     8761 atatgggttg cgacaaacta ttggtgacac catggggatt ggtgggattt ttagaaccct
     8821 acggacacta ccagtaatgt tagactttgc taaagaaatg gaagaagttt gtccaaatgc
     8881 ttggttattg aactatacaa atccgatggc gatgttaacc atgggtgttt taaaagcgac
     8941 gagtattaag actgttggtt tatgtcatag cgttcaagta tgcgtaccag aattgtttga
     9001 gcatttagga atagccgatc aatatgactt aaatgacttt caatggaaaa ttgcaggcat
     9061 taatcatatg gcattcttgt tggaaattac aaaaaatggg gaagatttct atccaacaat
     9121 tcgtcagtta gctgcagaaa aaccaaatcc acatccagat tctgtacgtt ttgaattgtt
     9181 gaaacgtttt ggttactata ttacagaatc aagtgaacac aatgcagaat accatccata
     9241 ctttattaaa tcaaaatacc cagaattgat tgacgaatta gggattccgt tggatgaata
     9301 cttacgtcgt tgtgaacaac aaattaatga ttgggaaagc atgcgagagg acattgtaaa
     9361 taatggggag ttaacacata cgagaagccg tgaatatgct tcatatatca tggattctat
     9421 tacgacagga actccgtcag tgattgcagg aaatgtctta aataaaggct taatcacaaa
     9481 cttaccgaca gatgtttgtg tggaagtacc ttgtttagtc gataaaaatg gcgtgcaacc
     9541 aacctatgtc ggtgaattgc caacacaatt agctgcttta aatcggacga atatcaatgt
     9601 tcaagaactg acagtagaag ccgctttaac actagataaa tcaaaaattt atcaagcagc
     9661 gtatttagat ccacatctat catctgaatt gtctttaaca gatatcaaaa atttagtgga
     9721 tgatttaatc gaagcacatg gggactattt acctgaatat aattaaaaag aagctgaaag
     9781 agtaaggaat tttcaattcc ttactctttt cttagttaaa tttagtaaaa atattgacaa
     9841 aacacttagt aaacagttac attagtgtta acaaaaagaa aacgcattca aattgtataa
     9901 ggaggagcat aaaatgaagt ggtctaaaaa aattatttta gcttgtatag cactaggagc
     9961 aacgatctct ttgactgctt gtggaaaaaa tcaggaagat gcaagcggta aggtagaaat
    10021 agagtttttt aatcaaaaaa aagaaatgac tcaaacgatt caggagattg ctaaagattt
    10081 tgaagcaaaa aaccctgata ttcatgtaaa agttgtggat gtaccgaatg ctggtgaagt
    10141 aattaaaaca cgtatgcttg caggcgatgt accagatgtc attaatcttt atcctcaaag
    10201 tatagaattg aaagaatggg cgaaagctgg ttatttagag gacttaaccg aagaaccgta
    10261 tttagagaat atcaaaaatg gatatgcaca acgttttgct attgaggatc gtgtatatag
    10321 cataccatta accgctaatg tctatggttt ttactataac aaaactgctt ttgaggaaat
    10381 ggggattcaa gcaccagaaa cttgggcaga atttgaaaaa atagtagctg agattaaaga
    10441 tcaaaacaaa gtaccgtttg cgattgcagg agcagaaggc tggactttaa atggttatca
    10501 ccaactagct atagcgactg ctgctggtgg tgaagaggaa gcgaacaatg tttggcgttt
    10561 ctcagaagtt aatggaatta atgctgaaag caaagaaatg caaaaagatt ttaatcgatt
    10621 agatttgtta agagaaccaa aagctttgca aaacaactgg caaggtgccg gttataacga
    10681 tacagtagtt acttttacaa aaggtgatgc gttgattatg ccgaatggtt catgggcaat
    10741 gccgatgatt cattcacaaa atccagattt tgaagtagga acttttcctt ttccagcaga
    10801 tgaagcaggt caaagtctaa cgattggcgc aggagaccta gccttatcag tttctgcaac
    10861 ttctcaacat aaagaagaag ccaaacgttt tgtagaatat atgacaacac ctgaagcgat
    10921 gcagaaatat tatgatgtag atggtgcacc gtgtgctgtt gagggcgtta tcgagaatac
    10981 tcaggattca ccattaagtg gtttaactga acttgctttt actgatcgtc atttagtttg
    11041 gttagccaaa gattggaata gtgaaaatga tttttatacc ttaacaacaa attatttaca
    11101 caatggcaat caaaaagcca tgattaacgc attaaatgct ttctttaatc caatgaaagc
    11161 tgacggaagg taggaaaata aaatgaaaat aaaagaaatt ttaaatcgtt actgggcaat
    11221 catttttgta gcaattccaa ttattttgca gatgattttt ttctactttc caatggttca
    11281 aggtgctttt tacagtttaa ctaattggac tggtctgact tataattttg attttgtggg
    11341 tttgaacaat tataagattc ttttttctga tccgaagttt atcaaatcga ttggcttcac
    11401 tttaattgtg acgctttgca tgattgctgg acaaattggt tttggtattt tgattgcgcg
    11461 ggcgttgaac gcaaatatta aagggaaaac attctttaga gcatggttct tttttccagc
    11521 ggtcttatcg ggcgtggttg tttctttagt atttaaacaa atttttaact atggtttacc
    11581 aaaaattggt agtatgttag gcattgaatt tttacaaacc agtcttttag gaacaacatg
    11641 gggagcgatt tttgcagtaa tttttgtttt actttggcaa ggaattgcca tgccagtcat
    11701 cattttctta gcgggttttc aaagtattcc acaagaaatt attgaagctg ctagaatgga
    11761 tggggcgaat gataagcaaa ttttttggaa tattgaaatc acctacttat taccaagtat
    11821 ttcaatggta tttattatgg cattaaaagg tggcttaaca gcatttgatc aaatttttgc
    11881 tttaactggt ggtggaccaa gcgatgccac aacatctttg gggttacttg tttataatta
    11941 tgcttttaaa aacaatcagt ttggttatgc gaatgctatt gctttagtct tatttatctt
    12001 aattgggatt gtttcactgg cacaattaaa attatcgaaa aaatttgaaa tctaggaggt
    12061 ttcccaatga aaacatcaaa agaaaaaaat atttctaaat acttgctttt attattaggg
    12121 ggcttcatga ttttagttcc cttagtttca acgctattcg gttcatttaa aacaacaccc
    12181 gatattatga ataatttctt tcgttttcct aatccagcga ctttggaaaa tttccgaaga
    12241 ttgttagcgg atggaattgg tcattacttt tggaattcgg caatcatcac aatcattgca
    12301 gtagttgctg tgacagcaat tattcctatg gctgcttatt cactagcaag aaaaatgact
    12361 cgtgggaaag catacggatt gatgtattta tttttaattt taggaatttt tgttcctttc
    12421 caagtaatta tgattccgat taccgttatg atgagtaaaa ttggtttagc aaatatgtgg
    12481 ggattaatct tactctatct cacttatgcc gtaccacaga cactattttt atatgttggt
    12541 tttattaaac taaatgtgcc tatcagtctg gatgaggctg ctgaaattga tggggctagt
    12601 cactttacaa cttatttcaa aattgttttc ccaatgttaa aaccaatgca tgcgacgact
    12661 attattctta atgctctatg gttttggaat gatttcatgc taccattatt aattttaaat
    12721 cgtgattcta atatgtggac actaccattg tttcaatata attatacggg acaatatttt
    12781 aacgactatg gtccaagttt tgcttcctat acagtaggaa ttgtcacgat tacactagtt
    12841 tatcttgctt tccaaagaag tattatagct ggaatgagca atggagcagt gaaataacca
    12901 ataaattatt ctcattataa aggagttcat aaacttatga ttatatttga caatcacaca
    12961 caaacatttc atttacaaaa taaaaaaatt agctatctat taggaatcga agaaaataat
    13021 tatttaacgc atctttattt tggaaaaaag attacgcact attctggtgg gtatcgctac
    13081 ccccgaacag atcgttcctt ctcacccaac aatgcaaatg cgcaagatcg tttattatct
    13141 ttagacacat taccattaga atttccagga tatggacatg gtgattttag agaaccggcg
    13201 cataatatta aattaggaaa cggtagtcgt gtaaatgatt ttcgttatga tagctacgaa
    13261 attcaaaaag gaaagcccag cttagcggga ttgccagcct tatatacgga agatgatgat
    13321 gaagctgaaa cgctgattat tcaattgaaa gacgcgatta gtggattgac tttacagttg
    13381 ttctacacaa tttatcaaaa tcacagtgta attattcgtt ctacgcaatt agtaaataat
    13441 agtgatgaaa cagtggagat taaccgctta gctagtcaat caattgattt tccaaatcgt
    13501 ccactcgagt taattcattt aaatggtacc tgggcaagag aacgtcaaat gactagagaa
    13561 aaaattcata cagggataaa agtattagat agtaaacgcg gatcaagcag ccatcaacaa
    13621 aatccgtttg ttgctttagt agatgccaaa acagatgaat ttcaaggaga ggcatatgga
    13681 tttaatttag tttatagcgg taatcacgaa acggtgattc aaaaagatcc atttgaccaa
    13741 acacgggtca ttatgggaat taattcattt aattttggtt ggcaactaaa aactggcgac
    13801 tcatttcaaa cgccagaagt cgtgatggtt tatagtaatg aaggtctgaa tacgttatca
    13861 cagacgtatc atgaattgta taatcaccgt ttaattcgtg gaaactatcg tttaaaagaa
    13921 cgtcccacgt taatcaacaa ctgggaagca acgtattttg attttaacga agaaaaaatc
    13981 atgaatattg tcgatgatgc gaagaacttg ggcattgaaa tgtttgtttt agatgatggt
    14041 tggtttggcg gtcgtgaaaa tgacttcact tcattaggtg attgggttga aacagaaggc
    14101 aaacttgctt gtggtttgga aaatttagcc aagcaagtgc atgataacgg gatgaaattt
    14161 gggatttggt ttgaaccgga aatgatttct gaagatagtg aactatttcg agcacatcca
    14221 gattgggcat tgggtattcc aggtcgtgac cgctcaatta gtcgaaatca atatgttctt
    14281 gatttttcaa gacaagatgt tcgtgataat atctatcaac aaatgacggc gatttttgat
    14341 cgtgtaccga ttgattatgt gaagtgggat atgaatcgaa atatgacaga agtctattct
    14401 gtcttgttgg actcaacgat gcaaggtgaa gtggctcatc gttatatgtt agggttatat
    14461 gaatttttag aaaagttaac aacgaattat ccaactattt tatttgaaag ttgttcgggt
    14521 ggaggcggtc gttttgatgc aggtttcttg tattacatgc cacaaacttg gacgagcgac
    14581 aataccgatg ccgtggctcg tctgaaaatt caatatggaa ccagcttagt gtatccaatt
    14641 tcaagtatgg gtgcgcatgt ttcagcagtc ccaaatcatc aaacaggaag agaaacgagt
    14701 ttaactattc gtggcaatgc tgcgatggct ggtgtttttg gttatgagct agatctaacg
    14761 caattaactt ctgaagaaaa agcagaaatt aaagaacaag ttgcctttta taaaaagcat
    14821 cgccaacttt tgcaatatgg aactttttat cgcttggaaa gtccgtttga aggcaatgat
    14881 actgcatgga tgtttgtctc aaaagaccaa acagaagcca tggtctttta tttccgagta
    14941 ttagcagaag ctgcgcatcc attagtcaca ttgaaattag caggattagc agaagaagca
    15001 acttatcaaa tggaagatgt aacatttgct ggcgatgaat tgatgaatct cggtttttat
    15061 attgactcag aactacatgg tgattatgcg acacaacgct tttatttgaa acagatatag
    15121 tagcttgaat ttacaatata agtgcaacac caaaagagac tcattccaaa cgaagaaaat
    15181 atgttttatt tacacgcatt tactaaaaag cagcctgatt tgaattggga aaatgaagaa
    15241 gtacgtgaag aaatctatgc aatgattaac tattggttag ataaaggact aggcggattt
    15301 agaatcgatg cgatattaaa catcaaaaaa aaactagagt atggcatctt tgaatctgat
    15361 ggagaagatg ggttagcctt cattgggccc tggattctta atcagtcggg cattgaagtg
    15421 tggttaaaag aaatgagaga gcggacattt aaaccacata atagtatgac ggtagcagaa
    15481 gccgatgttc ctaatgaacg tttagatgaa tatattggtg aagatggcta ttattcgatg
    15541 gtttttgatt ttagttatac ggatattgat gtacctgaaa ctggtgaatg gtttaaagat
    15601 agccaatgga catggacgga tatgcgtacc aatattttta cgaatcaatt agttacgcaa
    15661 gataaaggtt ggggcgcctt atatttagaa aaccacgatc aaccacgttc aattaataaa
    15721 tatattccag aagaatggat caatgattat agtaaaaaaa tgttggcgac gctttttatg
    15781 ttactaagag gtaccccttt tatttatcaa ggacaagaac taggaatgac caacatagaa
    15841 atggcatcat tagaagattt tgatgatgta gctacgcatt ctcagtataa acgggcatta
    15901 gaatatggat tggcaccaga gcaagcattg aaagctgtca ataaacgaag tcgggataat
    15961 tctcgcacac ctatgcaatg gaatacgcaa aaaaatgcca gattctcaac ttctgataat
    16021 gtatggttaa aagtaaatcc taattatcca gaactcaatg cagaagctca gttaaccaat
    16081 caacagtcgg tattcaatta ttatcatcaa ttaattaaat taagaaaaaa tagtgtatac
    16141 aaagacgtgc taatctacgg acaatttcta ccagtgaaag ataaaaatga acatattttg
    16201 ctttatgaac gccaattaga agataaaatc gtattagtta tcttaaacgt tacagcaaca
    16261 gaacaagaat attatgtaga tgaacagtac caacaagtat taatcaataa ttatgaagat
    16321 gttttattag atcaaagtcg aaaattgcgt ttacgaccgt ttgaaagtat tgtattagct
    16381 aattatggag gacaattatg aaaattaaaa acgaagcaat gctcatcacc tatgcagata
    16441 gtttaggaaa aaacatgggc gaattaaaac aagtcttaga caaacattta caaggtgtta
    16501 ttggtggtgt acatctgttg cctttttttc catcgacagg tgatcgaggc tttgcgccga
    16561 gtgattatac gacagttgat ccagctcttg gaacatggga agatgtggaa gcattaggtg
    16621 aagattatta tttgatgttt gattttatga ttaatcacat ttctcgtgaa tcaaaatttt
    16681 tccaagattt caaaaaaaac catgagcagt caccatacaa agaaatgttt atccgtattc
    16741 atgaattttt cccagaaaat cgaccaacac aagaagatat tgatttaatc tacaagcgta
    16801 aagacaaagc gccgtttcaa acggtagaat ttgcggatgg tacgacagaa gaggtttgga
    16861 atacctttgg ggaagaacaa attgatttag atgtgaccaa agaagttgtg aaagaattta
    16921 ttcgagaaac catcaaagat atggcgaacc atggctgttc tttaattcga ttagatgcgt
    16981 ttgcttatgc gattaaaaaa ttagatacca atgatttctt cgtggaacca gatatttggg
    17041 aacttttaga cgaagtgcgt caagaagcag cgaaatatga cgtcgaatta ttaccagaaa
    17101 tccatgagca ttattccatt caaatgaaaa ttgccaatca tgattattat atctatgact
    17161 tcgccttgcc aatggtcact ctgtattcac tttatagtgg aaaatcagaa cgtttagcca
    17221 attggttgaa aatgagtccg atgaagcagt ttacaacatt ggatacgcat gatggaattg
    17281 gtgttgtgga cgctcgtgat ttattaacag atgaagaatt ggattacacc tctgaggaat
    17341 tgtataaagt cggggcgaat gtgaagaagg tctattcaag tgccaattac aataatttag
    17401 atatttatca aattaacagc acctattata gtgcattagg cgataatgac cgtagctatc
    17461 tgttagcacg tgcgattcaa gtatttgcgc cagggattcc acaaatttat tacgttgggt
    17521 tattagcagg aaaaaatgat attgaactgt tggaagcaac gaaagaaggg cgtaatatta
    17581 atcgtcatta ctatgattta gccgaaattg aagaagaagt gcaacggcca gtgattcaac
    17641 aattatttgc acttttaaaa tttagaaatg aatctgcggc gtttgatttg gatggaacga
    17701 ttgaagtagc aacgccatca gaatcagaaa tagtgattac acgtaaaaat aaaaacggta
    17761 gtgatagtgc aacattaacg gctaacttgg atacgaaaga atttatcatt gagtctaaag
    17821 atataaaaat tttcactagt gttgcattaa aattttaacc tattccatag aacaaaaaat
    17881 cctttatact agcacttgga tgacttatcc aagaccaata aaaaggactc atacatggat
    17941 aagtataaaa caaatacagc atttaataaa tggttttctg ccataaaatt aggaaaactt
    18001 tctgtcaact cccaaaaatg tatttttgat tacaattcgt atcgtaaaaa actgtctttt
    18061 gaagctgtgc tcaagctctt tctctacgca gttaatgaag aaaaagaaag tttgcgtgat
    18121 ttgagtacag ctcttatcga ccgatcgctc caactagaaa ccgatgtgtc atcgattagt
    18181 catacccaat tgtctcgagc atttaatgca cttgaaccta aagtgttaga agaaattttt
    18241 caacaactgt tagaaaaagt tcaacatcaa atcaaaccaa ccaagcgaaa tcatctgtat
    18301 ctgattgatt ccagtacgtt ttcgcttagt ttaaaacgtc ataagtgggc gactttccgt
    18361 caaacaaaat caggtgtcaa gttacactta aactattgtt atatggacga tactacgatg
    18421 tatccaactg attttacctt aacaaatgcc catgaacatg atctcaatca actaccagta
    18481 ttggtgaatc aaccagaggc aacttacgtg tttgatcgtg ggtattagga ctttgaaaag
    18541 atggatcaga tgcattggga tggctatttc ttcgtcactc ggattaaaaa aaacaccaaa
    18601 gttcacgtga tagatactct ggaaacatcg ccagaaacag aaattctacg agacgagctg
    18661 gttcgtttag gatctaaaac ctatctaacc gccaatttcc gactagtcac tgttcaagat
    18721 aagaacggac gtgtgtttca atttatcacc aatcgaatgg acgtctcttc aaaagaaatc
    18781 tctgatatgt atcacgctcg ttggcaaatc gaattgtttt tcaagcacat caaacaacac
    18841 atgaccatta aaaccttctt ttctcaaagt gaaaaaggcg tgcagaatca attgatttta
    18901 acgatgattt ccgctctcct cacgtttctc atcaaattgg agacgaaaac ggaaaaatca
    18961 gtgttccaaa tcaaacgatt ctttcgttat ttactgtttc agccctttga atgttgtcta
    19021 gaaaaattaa ttcccacatg agaaaaaaga aatttggctc gttacttttg gttttcagtt
    19081 gaataacaga taaaaaattt tctggaaaaa gtcatcctct gcgtcagcac agtttttttt
    19141 gacttatttt cagaggaaat agttttcaga atattctaaa atgttttatg caacactagt
    19201 gatatttttt agaattagat ttctaatgtg cattttttga gtattaagga gcaagtatac
    19261 aaagatttat aaagctgatt aaatttgaat aaaaatatgc aggaacgcta ctatttttag
    19321 tagtgttctt ttttatattt gaataaaaaa tgtggtggtt aaatggtggt tataacaaaa
    19381 aatgaattaa tgaaattagg ttatgggaag tatcagtctg agagaatcat acgacaggca
    19441 aaagcaatta tggcttcaaa aagctatacc tattacgaaa atagaagatt aggactagta
    19501 cctgttgaat ctgtagaaga agttttaggt atgaagatag aggtagagga ggattttcag
    19561 aatcatggtt aattttaaaa aagataaaaa tgggaaatat cattttactg ctagtttggg
    19621 attcgacaaa cgaacaggga aaagagtaca aaaaaacgta gaggggttaa aacagttaaa
    19681 gaagaaaaaa agcgtatacc gaacttgttt ctaattatgg aaaagaagca gaaacttctc
    19741 aaagtactat gttgtttgag gagttttttt ataaattatt cctaccttat tatcaaggga
    19801 aagtagaaga aagaacatat aaaaatcggt tatctacaat caaaaaaact tttcttattt
    19861 ctttaaaatg gagttaagag aaatacgtcc aattgacatt caaaagtggc aaaataattt
    19921 attgagcaaa tataaaaatt cttatgtaag aaacatgtat gggatttttc agatgtcatt
    19981 agatcgagca gttgttttgg gactacttga aaaaaatcct gcaaaagtta ttggaaatgt
    20041 gaagaaaagg aaaactacag ttgatttttg gacaaaagaa gaatttacaa aagttatttc
    20101 aactttttat attgatgatt tgtaccaaca ttttggtttt atttgtatgt ggttactttt
    20161 tatgaccgtt atgcgtttgg gagaagccac tgcattaact tggaaagata ttgattttga
    20221 aaataaaaac ttgcgtgtga ataaaacact ctattataag agagccaaaa cctatgagtt
    20281 tgtagaaccc gaaacggctt ctagtattcg tacaatcgaa ctagacgaca atacaattac
    20341 ctttttaaaa gcttggaagg agcgtcaagt ttcaatagga ggcatgtcgt ttgtactttc
    20401 ttataatgga acacctactc agaaatatac aatccgtcat attatgagaa gacatgcaac
    20461 gatcgttggt gttcatataa tccgcataca cggattaagg catagtcatg cttctttgct
    20521 tatatcacaa taaaaagatg cacttcgaat taaagaaagg ttaaaacatg ctgatgtaca
    20581 aacgacttta ggaacttatg gtcatttgta tctaaattca ttgagagaga taacaaatga
    20641 tcccacagat tatatccaaa ctaatttttc taaaatcaaa ccgacaccta tttacgataa
    20701 taaaaaataa atgataaaca aaagtaggat cacaccaaaa gaaacacact agaaaaacta
    20761 taatacaggc ttttctagcg tgttgtttct tatttccgcc tatttataca ttatactcat
    20821 aaagcattta atgcatcgat cgtagccgca ttgtctatat gggtatataa agttgttgta
    20881 ttggtacttg aatgtcccaa ttgttgggca gttaatacca atgaatttgt ctgttggtag
    20941 agtctagtag ctaatgtatg acgcagtttg tgagggctga cttgaattct aaagcctttt
    21001 gaatattttg ctaccatccg ttcgattgca ataccggata tacgcttggc ttctccttgg
    21061 taaattgcta gaaataaagc tttttcgttt tctgttactt tatatcgttc tttccgaata
    21121 gctagataat tagctaaata ttccattgcg aatgctgcta tattgacagc atctctttta
    21181 tttccttttc tagttactac cacattattc ttatctaaag ataggtcatc taaattaata
    21241 tttataacct cggagagtct caatccagat cctaaaatca gtgcattgat cgccaggtct
    21301 ctttctacat ctcgttgata atagatcaaa gctcgtttac tcaaagtatt tttatattca
    21361 ttggcaatat aatctagata attttgtgta tccttatcta gaaataattt tttctcaatg
    21421 gcattggcac gtgcagctaa cgtagtttta tctgtgatgg ttgctacttg atccatagga
    21481 ttattgtcta agtatggttt tcctagagga ttattggaac ttttcgaaag atatttaaat
    21541 agaaccttta atgccgtgat atttctattg atagtagaag tagatagacc taagttatta
    21601 ttatctttga gcttagtttt tgttgtctct ttttttacat tttcttttgt gcgacgtttc
    21661 aaatgagatt tgtacaagtt taggtcatag gcagttaact gtcttatcga ggtgatagaa
    21721 gtatcaggaa cgatagactc actcagaagc cattcataaa agagcttata attttttaga
    21781 tattctaaag tggttcgagg tgattgatct ttatcctcac gatcttgaac aaaatctttt
    21841 atataatcgg gcatatgaag tatgattgta tctatttttt gatatatttc tgtatttttc
    21901 atgtcattca tcctttcaat aagtagggga gaagagtatc tctataattg gactatacca
    21961 attatagaat gttttgtcaa ttggtatgct ctaaaattta ttaatttgtt tcaaaaaaag
    22021 catataaaca ggtatacgtt taataattta aaataaatgg tatacataaa tagggaagga
    22081 aatagaaggg agcaatctaa attttttgaa ttttcttaat taattatata gaaagaacaa
    22141 tctttctata gagttagaca tgagagggac tatgcttgat ttaaaatcaa caagcgtata
    22201 tactgtatat acaaagaaaa aggagggaga agactatggc aattaaagaa agaaaattta
    22261 gggaagtggg gaactcagta gtattaacta tttcaaagga gtttcttaaa gaaaatgggt
    22321 taaatcccgg agatacggtt tttttagatg aagaaaaact tcgagcggca attacaaaaa
    22381 aacaagcctt atctgatgaa attgatttag caatatctca agcattaact gaatatgata
    22441 ctacttttag agagcttgtc gataaatgag tattcattac ctagaagcca aagatattat
    22501 aaaaatgaat gtaatccaaa taaaaaaata ctctcctaat gaaccccttg gtgtaaagga
    22561 tgcgaatgct ttagaaatgt gtgtagggca acttcgagcc tcagcttttg gtgaagaggt
    22621 ttatccttcg atttatgaaa aagcagctat cctacttata caattgataa agaaacatcc
    22681 ttttcacaat gcaaataaac gtactgcttt cttagctact tttgtctttt tgaaaatcaa
    22741 tggacagctt ttagctattg atcaaaaaga tgctgtaaat ttagcggtat atattgctac
    22801 gtatgataag gatttcgatc aactaaaata tgaagtgatc aacactataa agacacatac
    22861 gcagcccatt taaggagtta ctgtatgtgt ctttataggg ttattagctg ctatagcgaa
    22921 ataattgaag taaattacaa atagctataa ttcaaatcca actatataga aaacgtaaat
    22981 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
    23041 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
    23101 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
    23161 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
    23221 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
    23281 tatgaaatga aagacctttt acatcttgca gaaatcgtta ctgggattaa actaagcaat
    23341 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
    23401 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
    23461 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
    23521 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
    23581 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
    23641 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
    23701 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
    23761 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
    23821 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
    23881 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
    23941 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
    24001 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
    24061 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
    24121 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
    24181 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
    24241 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
    24301 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
    24361 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
    24421 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
    24481 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
    24541 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
    24601 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
    24661 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
    24721 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
    24781 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
    24841 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
    24901 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
    24961 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
    25021 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
    25081 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
    25141 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
    25201 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
    25261 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
    25321 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
    25381 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
    25441 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
    25501 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
    25561 ccacttagct tggataggct acaattaact agacagaaaa attaaggtgt gtagactaga
    25621 agaaaacata ccaggaggaa tttttatgtc taagagaaca cgaagaactt tttcacaaga
    25681 attcaagcaa caaatcgtca atctttactt agctggaaag ccacgtgtag aaatcattcg
    25741 agaatatgaa ctaac
//
LOCUS       DAYYCW010000029        25478 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000029 DAYYCW010000000
VERSION     DAYYCW010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.029"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..293)
                     /locus_tag="ACHAYA_001128"
     CDS             complement(66..293)
                     /locus_tag="ACHAYA_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM8604940.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(290..808)
                     /gene="msrA"
                     /locus_tag="ACHAYA_001129"
     CDS             complement(290..808)
                     /gene="msrA"
                     /locus_tag="ACHAYA_001129"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8604941.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(828..1541)
                     /locus_tag="ACHAYA_001130"
     CDS             complement(828..1541)
                     /locus_tag="ACHAYA_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM8604942.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(1544..2440)
                     /locus_tag="ACHAYA_001131"
     CDS             complement(1544..2440)
                     /locus_tag="ACHAYA_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8604943.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(2598..3440)
                     /locus_tag="ACHAYA_001132"
     CDS             complement(2598..3440)
                     /locus_tag="ACHAYA_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8604944.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            3572..4225
                     /locus_tag="ACHAYA_001133"
     CDS             3572..4225
                     /locus_tag="ACHAYA_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM8604945.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(4288..4815)
                     /locus_tag="ACHAYA_001134"
     CDS             complement(4288..4815)
                     /locus_tag="ACHAYA_001134"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8604946.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(4835..5782)
                     /locus_tag="ACHAYA_001135"
     CDS             complement(4835..5782)
                     /locus_tag="ACHAYA_001135"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM8604947.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(5794..7677)
                     /locus_tag="ACHAYA_001136"
     CDS             complement(5794..7677)
                     /locus_tag="ACHAYA_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8604948.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(7840..9918)
                     /locus_tag="ACHAYA_001137"
     CDS             complement(7840..9918)
                     /locus_tag="ACHAYA_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8604949.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(9931..10206)
                     /locus_tag="ACHAYA_001138"
     CDS             complement(9931..10206)
                     /locus_tag="ACHAYA_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604950.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(10568..11023)
                     /locus_tag="ACHAYA_001139"
     CDS             complement(10568..11023)
                     /locus_tag="ACHAYA_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604951.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(11177..12385)
                     /locus_tag="ACHAYA_001140"
     CDS             complement(11177..12385)
                     /locus_tag="ACHAYA_001140"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM8604952.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(12497..12829)
                     /locus_tag="ACHAYA_001141"
     CDS             complement(12497..12829)
                     /locus_tag="ACHAYA_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8604953.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            12977..13852
                     /locus_tag="ACHAYA_001142"
     CDS             12977..13852
                     /locus_tag="ACHAYA_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8604954.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(13934..14515)
                     /locus_tag="ACHAYA_001143"
     CDS             complement(13934..14515)
                     /locus_tag="ACHAYA_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM8604955.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(14521..15780)
                     /locus_tag="ACHAYA_001144"
     CDS             complement(14521..15780)
                     /locus_tag="ACHAYA_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8604956.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(15782..16828)
                     /locus_tag="ACHAYA_001145"
     CDS             complement(15782..16828)
                     /locus_tag="ACHAYA_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604957.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(17063..17338)
                     /locus_tag="ACHAYA_001146"
     CDS             complement(17063..17338)
                     /locus_tag="ACHAYA_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM8604958.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(17581..18891)
                     /gene="der"
                     /locus_tag="ACHAYA_001147"
     CDS             complement(17581..18891)
                     /gene="der"
                     /locus_tag="ACHAYA_001147"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM8604959.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(19073..20302)
                     /gene="rpsA"
                     /locus_tag="ACHAYA_001148"
     CDS             complement(19073..20302)
                     /gene="rpsA"
                     /locus_tag="ACHAYA_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM8604960.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(20437..21117)
                     /gene="cmk"
                     /locus_tag="ACHAYA_001149"
     CDS             complement(20437..21117)
                     /gene="cmk"
                     /locus_tag="ACHAYA_001149"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM8604961.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(21166..21801)
                     /locus_tag="ACHAYA_001150"
     CDS             complement(21166..21801)
                     /locus_tag="ACHAYA_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM8604962.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(21867..23270)
                     /locus_tag="ACHAYA_001151"
     CDS             complement(21867..23270)
                     /locus_tag="ACHAYA_001151"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM8604963.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(23257..24288)
                     /locus_tag="ACHAYA_001152"
     CDS             complement(23257..24288)
                     /locus_tag="ACHAYA_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8604964.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            24319..24540
                     /locus_tag="ACHAYA_001153"
     CDS             24319..24540
                     /locus_tag="ACHAYA_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM8604965.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(24705..25442)
                     /locus_tag="ACHAYA_001154"
     CDS             complement(24705..25442)
                     /locus_tag="ACHAYA_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM8604966.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
BASE COUNT         7046 a         5350 c         4335 g         8747 t
ORIGIN      
        1 aataattctt tcaattaggc tattggacaa aaaacttttt tgatcataaa gtacaaaact
       61 atctcttata tgtcatgatt attttctaaa tatttttccc atgcttcgtc aaagatatcc
      121 atggacggca aatagtcgac attcagttcc agataattag aaatttcttc gtagctagag
      181 ctgtgcttag gaaattggat gtctttaccg gcttctgtag cgaaaacaga taaagcatca
      241 tgtgtgccac cgcctcgcag tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt
      301 tcctccaatt ttcttcgata aaggcagccc ggcgtgcatg gtttctttta aaattttcag
      361 gatgcttttt ataatactct tgatggtctt cttctgctag ataaaatggt tgggctggtt
      421 cgatttttgt aacgattggc tgttcgaaac gtccactttc agctaactgt ttcttacttt
      481 tttcagcaat gatccgctgt tcttctgtca aataaaagat caccgggcga taattgtcac
      541 cgcgatcctc gaattgtccg aaagcgtcag ttggatcagt ttgctgccag taaagttcta
      601 ctaattgttc atatgaaatc aatgttggat catagataat ctccactgct tctgtatgtc
      661 cagtcgtttg gctttttact tgttcataag tcggatctgg tacatgccct ccagtatatc
      721 ctgacatcac tgtatggata ccaggaagcg tgtcgaaagg ctggatcata caccagaagc
      781 agcctcctgc aaatattgct cgttccatta tgattccccc tctaatacta ctcatccgat
      841 gattttttat tttcttttgt cttgctttca gtactgctgg tgctagtttt gttggtattt
      901 gagttgtttg aagcatcaga ggtatctttt ggcaaatata gactaaaccg aatgtcatca
      961 tttactaagt cgattttatc tgctttaata tacatcccgt tttgcattcg gaattgatcc
     1021 aacctaagca tgatagtcag atcatctgta ttgacttcga tccaatctgg taatttatag
     1081 tttctcttga tcatattcat cacatctttg attggcaaac tcaatgttcc gatagaaaga
     1141 cttctagctt ttagttggac atttccgttt tccatcacat acggatcaaa atataaatag
     1201 aaatcgatag gaaaacctag tacttcgaaa gtaccattga gtagtgcttc attttccaaa
     1261 taaaatttat attcaatatc actatctttt tgatactcac taaggaaaaa gctgatgatt
     1321 tgattgactt tttctttatt tgaattgatc gttaagacag gatctccttg tctttccgta
     1381 agagctggta tttctgggag ttctggttcc cgattagcaa aaatacggct gcctaaaaat
     1441 acggtcgaac caagtactac cccaagaagg ataaggaagg cgaatttcca gtaatttgtt
     1501 ttctttgttt tgtttgaaga tacgtcagat cttgttttca tgtttattga tcatcctcct
     1561 tctctaacca ttggttttgt gtctcgatca gctgatctct cactgcattt gccatcaatt
     1621 gataacctaa attatttgga tggaaatgat cttcttcata gagcacatta tttttgatgc
     1681 catttagatt ttctgtcgtg ctagttgttt gatctgttcc ttcttctgtg acacctactt
     1741 cctgattcaa tccttgataa agcaaatcat tgattgggat aaagtagcag ttcggcattt
     1801 tttttgtcac cgtttctgtt cctttgttcc aattgttaac aattgtctgc atgtcagtga
     1861 tctcaggaaa gttcaaataa aatggattgt agatgcctaa aatgtaaata ggtgcggttt
     1921 tattgaggga tcgaaattct tccaatagtt ctgtgacatt ttcctgatag tttttcagtg
     1981 gctttttaaa ggaattgacc gttaacccga ataaattatt ttggataact ttcatcaaat
     2041 cattaccgcc aacagtcagt gtgataaaat cagctgtttt gatattgttt cgaatttctt
     2101 cttttgtttt tactcgcttt aagatctggt cacttcgttc accatttaca ccatagtttt
     2161 ctgtttcaac cgcggtcaaa ttataacggt cttttagatc atttgcgaca aggggaacga
     2221 atcctccttg cttggtttga tctcctactc cttcagtcag cgaatctccg atagctatgt
     2281 agtgaagttt ttctttttta tttggatctt ctctcacagt agttactggt ttcattagtg
     2341 ggccagcttt cggtatagaa gcagctaaac ctgcatagat gatgatcgca attaataatg
     2401 ggaacaaaac gaggatcaaa tgtcgtttcg ttaatttcat tttttcacct cttgtattcg
     2461 attggttcta atgttatcaa aaattttatc aaaaaggtag tctttatgaa aaaagaaaac
     2521 aattattttc ctaattttat aaagaaaaag agaataagct gcaagccttt caggctgagg
     2581 gcttatcctc ttttcgacta atctacataa tacatgattg caaacgcgtc tttaccagta
     2641 tgcgtagcga taaccggatt cgtatgcagt acagggatat gcatgtccgg gaataattct
     2701 tgcaaaccct ctttgaattc ttgcgccaat tcttttgcat ccgcatgtga aataccgatt
     2761 tgtcgcacat ttggcaattc tttcagttca tttttcaaat tattaaacca tttatcgaac
     2821 gttttctttc ctcgcccctt agttacaggg attaattcag aatgggcgaa attcatcacc
     2881 acgcgtatgt tcaaaacact agacagcaaa ccagttgttc ggctgattct tccacctttt
     2941 accaagttat ctagtgtgga tattccgatg tataattttg tattgtcttg tacttttttg
     3001 acttcttcaa ggattttctc tactgtttta ccttcttttg ctaattcagc tgctcgaatt
     3061 acctgaaaag ataatccttg gtcagtggta tcactgtcga taaccgttac tttcgttttt
     3121 gttagattac tggcttgtct tgctgcttct actgttccac tcaagccttt tgtcatatgg
     3181 atcgaaatca cttcactgcc gtcttctcct agctcatcat acatcgagat gaattctcca
     3241 attggtggtt ggcttgtttt gggcagattt tttgctttcg ccatcatttc cataaatctt
     3301 tcgccgtcta gttggtcatc atcgggatat actgttccgt cgatcatgat ggatagcggc
     3361 atgacacgaa tgcctaaagc atcgcgtaca tttttttcca tcgtacatga agaatctgtt
     3421 acgattttta catttgtcat tattttctct ctctctttct ttaacaagat gccttattca
     3481 tgttaaacta catgtaagac aatttctatt tttagtataa cagacaaata atgatttttc
     3541 attaattaca cctagtaaag gagccgtttt catggaaaaa acgaaattta atcgaacgta
     3601 tgaaatatta aatgaagtgt ttaatgcagt cacccatgga attgggactg gattgagtat
     3661 cgcgggacta gtggttcttt tagtaaaagg tgcccacttg cattcctccc tccatattgt
     3721 ctcttatgct atctatggat cgatgatgat tcttttattt ttaacgtcta ctttgtttca
     3781 ttcattgatt tttacaaaag caaaaaaggt gttccaagta ttcgatcata gttcgatttt
     3841 tcttttgatt gcaggaagtt atactccttt ttgtttgctg agtatccgag gatggctagg
     3901 ttggacttta tttgtattaa tctggttgtt tgcgattctt ggcattatat ataaagcgtt
     3961 gacgttgcat aaacgggaga gagtatcaaa agtatctacg atcatctata tttttatggg
     4021 gtggctctgt gtcgtggctg ctgttcctct ttatcactct cttggaatga ctggtgttat
     4081 tttgatggta gcaggcgggc tttcttacac gataggcgca ttcttttata gcttgaattc
     4141 tatccgctat atgcatgttg tttggcattt gtttgttatg ttaggtgcag gttttatgta
     4201 cttctctgtt ttattcttta cttaataaaa aaaataaagc tatggatggg acagctctaa
     4261 ataacagcta gtctatccat agctttttta tatcgtcaat tcgcctgtcc cgctttttac
     4321 aggattctca ttacgctgat acgtttcaaa ttgatgcgga taacgatttt tttcatccat
     4381 ttctccttga ctagtattga tcagtgacca atcagaccag tcaacaggtg gaaaataagc
     4441 atctccatcg aatacatgat ggatgactgt acggtataat atcgtacaaa atggcagaaa
     4501 ttcttgataa acagctgacc ctccagcgat gaaaaagata ccgtctgttt ctttttccag
     4561 agcaagcacg tcttccacac tatgcatgac atgcacgcct tctgctttat aggaagaatc
     4621 tctcgtcaaa acaatcgtct gacgattcgg caacggacgg ctacccatcc cttcgaatgt
     4681 ttttcgtccc atcactagtg tatttgcttc tgtcatttgt ttgaagaatt tcagatcatt
     4741 tggcaaacgc caaggaagac ggtcttcttt tccaatcaat ccgttttcat cctgtgccca
     4801 aattgcagct aacattttct catcctttct gattctaaac agcgatagga gctttgatcg
     4861 ccggatgtgg gtcatatcct tctaattgga tatcttccat ctcaaaatca aatactgact
     4921 gcttttcctg attcaaaact aaagtcggaa atgcgcgtat ttctcggctc aattgttctt
     4981 tcatttgttc gatatgattg atataaagat gagcatctcc taatgtgtga acaaattcgc
     5041 caaccgctaa acctgtttca tgtgcaatta ggtgagtcag caacgcataa cttgcaatgt
     5101 tgaacggaac gcctagaaac acatccccac tgcgttgata caattggcag cttagccgtc
     5161 cctcttgaac ataaaattga aacatcgtat gacaaggagg caaagccatg ctaggaacat
     5221 cttccggatt ccatgcagaa acgatcaatc ttctagaatc aggagtcttt ttgattgctt
     5281 cgatcacttg tttgatttga tcgataaagc caccgtcttt tgtttcccag tgacgccatt
     5341 gatagccgta gatgtgtcca agatcgccgt atgtttcagc aaaggaatca tcggttagga
     5401 tcttctcgca aaaaagcttg ctctgctttt tatattcttc attgaattca ggatctgtca
     5461 aacatctatg gccaaaatca gtcatatctg gtccattata gtctggactt ttgatgtagc
     5521 gttcaaatgc ccattcatcc caaatatggt ttttattttg taatagatag cggatattcg
     5581 tatctccttt taaaaaccaa agcaattcgc ttttgatcaa tccaaaaggt actcgtttcg
     5641 ttgttaacaa aggaaaccct ttttgcagat caaaccgcat ttggtatccg aataagctta
     5701 atgtgcctgt gcctgttctg tcttctttac gatgcccttc ttccaacaat tttcttccta
     5761 agtcaagata cgcttgttcc atcctttttc ctcctaatct tctacccatt cactcaagta
     5821 ttcccaccgt gccatttttt cttcaagttg tgcttctgtt tcagatacgt cgttttgcaa
     5881 ttcttgtaat cgagtgaaat tatctccttg atgattcatc tcttcttgga gcaatgagat
     5941 tttttcttca agttctgcaa tctcatcttc aatcgtttcc cattcttttt gttccatgta
     6001 agataatttc ttcttttcag caggttcctt gctctgtatt tttggtttcg ctttttcagc
     6061 aggctgctgg gtctcttttt gcttcagtaa ataactggac atgtttccgt aataaagttc
     6121 ttgttttccg tttccttgga atactaataa cttatctgct accttgtcca agaaataacg
     6181 gtcatgggaa acagtgatca cagcacctgg aaaacttcta aaataatctt ccaaaatcgt
     6241 caatgttgct atgtccaaat cattggtcgg ttcatccaat aataaaacat taggttgctg
     6301 aatcagcagt ttcaacaaaa ataaccggcg tttttctccg cctgaaagtt tgcctatcag
     6361 tgtcccatgc ataaatcttg gaaataaaaa tcgttcaagt aattctgcta ctccgatttg
     6421 actgccatct gctgtttttg cttcttcagc agcttcttgt aagtaagcaa tcatccgttt
     6481 atctggtgcc atttcttcat tttcttgtgt ataatacgca agatgaaccg tttcaccgat
     6541 agacattgta ccgctatcca ggggaattct gccagctaga atattaagca atgtcgattt
     6601 tcccgcacca tttttaccag taatacctag tctttcccga gattgaatca ataaatctaa
     6661 atgttctagc agtgtttggt catttatacg gtaactgcca tctttgattt ccagtacttt
     6721 tttccctaaa cgttgtgtcg ctaggttcag ctctaaatca tcttcttggt ttacttggta
     6781 caagttctcc tttagatctt cgaaacggtt gatccgtgct tgctgcttgg tgccacgtgc
     6841 ctgtacgcca gccctcatcc aagctaattc ctgtttataa agccgcttgc gcttttcttc
     6901 ttgttctact tctacacgat cacgttctgc tttttctaag acgtaagctt cataatttcc
     6961 tttatactcg tataattttc caaatgaaag ttcgaagatt cggtttgcta ctctatctag
     7021 aaaataacga tcgtgggtaa ccatcaatag tgcgccacgg tattgtttca aatagttttc
     7081 caaccactcg atcgcttcat aatctaaatg gtttgttggt tcgtctagta atagtaaatc
     7141 gggttcatca atcaacactt gcgccagact aactcgtttt ttctgaccac ctgataaagt
     7201 actgattttt ttatctaatt cactgattcc taatttttgc aaaataatct ttgcgttggt
     7261 atctgtagtc caagcatctt ctttattcat ttgttcttct gctttagcat aacgtttttg
     7321 cacatcaggg tcttctccgt tttcccctag ttcgatcaat gcttcctcat atgctcgaac
     7381 agtttggatc aaggggctgt ttccttgaaa gactgcttgg agaacagtta attcatcgga
     7441 aaattcagct gcttgagaaa gataaccaat acggtaatca gttggataaa acactgtttg
     7501 ccgatcaccg tcgctgctat cgataccggc aataatattt aagagacttg ttttacccgt
     7561 cccgttcgtc ccaatcaacc cgatacggtc tttttcgtgg accaaaaaag aaatctggtc
     7621 aaacaaatct ttttcaccat aagttttttt gaggtcagtt acttttaatt ctttcaacat
     7681 gttccctcct tatcttggat attgtatcat aaaaatagtg aacttcagga aaattttttt
     7741 ggtatttgtt ctttttgtgt tctcaaaaaa agaacaggac cgattcgatc ctgttctgat
     7801 atgttttgtt ttttataaaa cttgaaaatt tttacctttt tattttattg gactcgtttt
     7861 ttttgacttt gctcgaaacg caaatcggaa agaaaagtta tcagatggtt tgttcatcgt
     7921 ataagattct aacaaattga tattcctgcg atagctgttc gcagaaaacg tcgtgatttt
     7981 ttcagcaact tcgtattcat caatgatttt tctttcaaga taataacctc ttagcatttt
     8041 tttcgtaaac agactatctt gctggatcat ccacgaagaa atcaaattcc ccgagttcat
     8101 ttttattcct tcaagtttgc gttgctggat aaaaaattga accatcgttt catcataaat
     8161 atcagtcaag ttatctaaac tttcaataat caactgagta tttttttgat atgtctctct
     8221 gatttcttgg atatcttcat cactaataaa tgtctgacga tcagcatatc ttctttgcca
     8281 aaacattttc ggatggagaa cgattcgcat taacctacgg acaacaatga accaaaagcc
     8341 gataaaagac aaaatctggc ttgtgataga atgttcgaat cgagaaagaa aacgggagta
     8401 aaaacgataa ccattagaag aaagcttgcc cgaacgataa ctttcatcca atccatcccg
     8461 ttcgatcgac aaaatcagtg cttgtatttc ttgtacttct tgcttttcat catcggttaa
     8521 atcttccaag tacagatcac gtatccgttc ttgataattt tctatcactg cttcagtggc
     8581 taaaaattct tttgtattac gatcaatttc ctttatttct tttcgcaaaa cttcaacgac
     8641 ctcttctaaa atcatcaaag cattcaaatc cgtgcttttc gcttcctctg tttccgtcag
     8701 cataggcaag acaatgatcc ctatgaccaa agtcactaag atgacacccg ccgtcagaaa
     8761 caaaagcaaa gaccgctggt aaaacatcat attattgatt gaaaatggca aaataaaaat
     8821 cgtagccaaa ctgactgttc cctttacacc gccaaatgtt aagatcaaga tctcgttcaa
     8881 ctgttttttg aactttttac tgccatcttt gaatacataa aaaagactga tagaaatgaa
     8941 tctgatcaca aaaagcatga cagagattaa tacgataatc gctaataaaa gaccgtttgg
     9001 atataaaccg tctccccata ctggcgagaa cacttgcgtc aattcgattc ctaaaaatat
     9061 aaatactaat gcattcagtg taaaagcaat agttgtccat gtactgtggg acaagctcga
     9121 caattctgca tcaaacacag agattttcct aaaaccagaa gcttggagaa tacctgctgc
     9181 cactgcagca atgattccag atacatcaaa tgcctctgcc aatacatagg ctagaaaagg
     9241 aagcaaaagt tcgatcagca gataccctgt gacatcttgt gctgatgctt tttcgatcaa
     9301 atgaattatt ttttgtttga accagacaag caaaaagccg atcaaagctc ccccaatact
     9361 agaaactact agactcatcc ccgcgtttac tgctgaaaaa gatccggtga tcagcgcacc
     9421 aagagcaaac tggaaggcag taacaccaga tgcatcattc aaaagacctt ctccttccaa
     9481 aatatgcatc gcctttttag ggatattcac tcgtccagaa agagaactga ctgctaccgc
     9541 atctgtcgga cctaacgctg ctccaaaagc aaagcaggca gcaagtggga cacttggcaa
     9601 caggaatgat aacgtcgcac ctaccccgac aagggtcaaa atgactcctc caaatgccaa
     9661 gaacaaaatt gtcccgaaat ttttgagaat cgaagaaata tctgcttttt ctccttctct
     9721 aaataacaat ggtgcaataa tcataactaa gaacaattca ggttcaaaat cgattgattg
     9781 tccccactct gtcagaccta ataccactcc aaggaaaatt tggatcagtg gagctggaat
     9841 cattggcaaa atgcggctag ctatgttcga taaggtgatc gttactgcaa atacaataac
     9901 taactcagca aattccatcg tccatcccct ttatttttta ttttgtgtaa gcatttttaa
     9961 aaattgttct tttttcttta aaattttggt ttcgactact tcaacctgtg tttggttccc
    10021 ctctaattca cactgtacac gttttgactc caaacgaagc agatcatgtt tcagacgttc
    10081 caactgacga tacatcaata tatttctttt acgcaactta caaagtattt gacggcggtc
    10141 atccatcgaa agggcgccat agcgatcatg caacagtttt ttttgttgtt caaaactctc
    10201 tctcattttc cagatctcct atacacaaac ttccatcatt atagcaaagt tctttttgga
    10261 tgaaaaatat ttcattgttt catcgtttat tgctagttag aaatttccat aaatttttca
    10321 acgcctcatt ctattccaaa tagtatcaga tatcaagtaa aacttaagaa agataaaagt
    10381 ttctgacaaa aggatgttta ctttcccatt cctgcctttc ttgttctttt atcacaacaa
    10441 aaaaatgggg ctgcgacaaa aatcttgtca cagtccctta ttctgaataa acggtgtgga
    10501 agagacagcc tatcagtagt aataagacac atctctttca cagcctcatt tttgcgtaaa
    10561 aatcgattta ttcgaaaaat tccccgtata catcttctac acgttgatct ggattgatca
    10621 cgatatacaa gttacctgtt ttttcgctga tttttgttga ttgatattga ttgtctaatc
    10681 cttctgttcc tttatctaga tatgggaagt acacaagatc aggtgtgcca tttcggtaaa
    10741 ggatttccat tcgctcgata tcttcgtatt tcaatgcgcg attaaacatg cccatttcca
    10801 gtccgcctag gttgatatca tgtgtcgcaa ctgcgtcccc ttcatggaag atttcgattt
    10861 taaatccagc acacggatgg atttcaacaa attcactgcc attgattcgt ccaaaacttg
    10921 ttgtgatttg tttgatccaa agatcgccga tatgacggcg gttgatcgtc caggtttctc
    10981 catttctaaa ataaaatttt agcgctgtca tttctttact cattaaaaag actcctttat
    11041 gatgtaattt attcttgtac tacctcagtt tagcacaaat ttatgtaagt ggtcacatct
    11101 tatgtttttt cttctaatga cgtatctgcc cactttaagt agccaacaaa gctgagcaga
    11161 tacagtctag tgttttttaa atcaattttt ctttcgataa aaaatccaaa acctgttttt
    11221 tatcattttt tattctttgt tctacgacgg ctttttcagc taattgcaaa gcttcactaa
    11281 cccacttacc aggttttttt ccagtttctt ctagcaagat tttaccatct actgctaatt
    11341 cgcttaatga cttgataggt aaacgatcat aggattctcc tacggcatcc atctgaggtg
    11401 tttcgtcaaa atagatcatc aattcttcta cttgcagtgc atgttttttg cctaagaagt
    11461 acaattcgaa tggttccaaa gctccagttt ctcgttgtcg tagtccttgg acaagtgctt
    11521 gaacgtcttt aatcatttgg ttagaacact tccacgattt caaaaaggaa cgaatctcat
    11581 tttcttttag atccagttgg ctgatcaata atgcccatgc ttggctctct tctttcaatg
    11641 ggacggatgg taattcagca aaacgtaaaa gtgcttcgcc ttcttctttc aaaccgggac
    11701 agcaaatgta gcactctgtt tcaataaatg catggattcc cgcggcccgt tcatttccca
    11761 agagcatctt gatgaattcc acatttatac gttctacaga tattttacct aatagctcgt
    11821 ggttttcttt gatggcgtag aaggtttctt cctctaattt gaaccctagt tgactgacaa
    11881 aacgcaaccc gcgcatcatt cgtaaggcat cttcatggaa ccgctcatga ggattgccta
    11941 cagctcgcaa gatctgttct tctagatctt gcagaccatc gaacaaatcg atgatttctc
    12001 catcttcttt caaagcaaac gcattgatcg taaaatctct tcttttcaaa tcttctgcca
    12061 acgaacgaac gaattcgaca tgatctggtc tacggtagtc ctgataagtt gattccgtcc
    12121 gaaatgtggt gatctcatat tgttcatcat tttctaatac caacacagtt ccatgttcga
    12181 tcccaatatc tatcgttttt ggaaaaagct gtttgatttc ttctggaaat gcgcttgttg
    12241 cgatatccac atcatggatt ggctggttca gcaagatatc acgaacactt ccacctacaa
    12301 aataagcttc atatcctgca ttctctaatt ttttcagaac tggaattgcc ttttggtatt
    12361 ccagaggtaa ttgttttaat ttcatttttc caacctttcc tgcgtgtttc ttgtcctgag
    12421 ataaagactc agttgcatgg tgactgtttg acaagtccgt ttcagagtct tcaaggccag
    12481 gaccacttca tcttgtttac tcgtccttac gctcaaagcg attatggtct ctttgattga
    12541 atttttccat gtttttttca aaaacttcag ttaagtcgat attcaatgaa ttagccatga
    12601 tcatcgtaac gaataaaaca tcaccaagtt cttctgctac cgagttaact ggttcagaag
    12661 cctttttgct tttttctcca taagtgtggt tgacttctct tgccaattca cctacttctt
    12721 cagttaatcg agccatctga gctaatggag agaaataccc caccttgaat tgctggatat
    12781 attcatccac ttcctgctgc atactgtata aagaacgctt tttatccatc tttttccctc
    12841 caaaactatc ttaccaaaac tttaacgcaa atcctagata agagcaattg agaaaatcga
    12901 atttccatgc tacatttagg aaggaatttc taaatggaaa ttctgtgaaa atacataatt
    12961 gaaagctgga attcatatgg tcaaacgaaa aacagtaaaa gaagttcttt ttatcattat
    13021 aggaacaagt atttatgcat ttggtcttgt ttacttgaac atcgctaatc atttagcaga
    13081 agggggagtc tcagggatca ccttgatctt aagagcttta tttggtattg atccagctta
    13141 tacgacttta ttaatcaaca tcccattgat tttactagga ggaaagatcc tcggtaaacg
    13201 ttccttggcc tatactgttc taggtacact ttctttgtca ttctttttat ggatctggca
    13261 aagggttcca ctagaaatca atttacagca tgatctactg atcgtttctc tacttgcggg
    13321 tttagttgca ggtgtaggaa gcgggatcgt ctaccgcatg ggtggaacaa caggcggcag
    13381 tgatattatc gctcggattt tagaacaaaa ttacggcatc actatggggc gttccctatt
    13441 ggcatttgat atcatcgtct tacttgcttc attaacctat atcgacttga aacgtatgat
    13501 gtatacgttg attgtttcgt atgttttcag tcgtgtgatt gattcgatct tagatggcgg
    13561 atattctgca aaaggtctac ttgtcgtctc caacaaaagc gaagagattg caccactact
    13621 gatgaccggt ctgcaaagag gtgtgacttt cctttctgga gaaggcgcat tctctggtaa
    13681 ttcaaaaaaa atcatctata tggtcgttag ctctagtgaa ttaactgaag ctaagaggat
    13741 cgtccacgag attgacgaaa aagctttcct ttcaatcatc aatgtgcatg aagtggaagg
    13801 gcaaggcttt acttatttga agccacagac aagattactg aaaaaactat aagaaaaaag
    13861 acaaagatag gctatgagga aagattccac atccagctga cctttgtctt ttttagtact
    13921 atactatttt taactaattg ttatgttcac ttacccggtt acttttcttt tgtgtctgca
    13981 aaaggatctt tcggttaact tcatcagata atttcaaaaa agcatcccta tctccatctt
    14041 ctagtgcttg atcgatttga gcataaagca tttttagttt tgcctcttct tctttttctt
    14101 gaaaatatcg ttcaattaat tcagtatctt cttcactgac tgaagaatcc caagaagaaa
    14161 atggattgtc ttctaagata gatagatatt ctcttgtctg ccaagcattt tcgaatatac
    14221 attccacata taaaggcttt ttccaattca aacggatttc atgaaaaatc tggtctgtgt
    14281 ccgtaaattc ttttccctgc aaaaacaatt tcatgggttc cccttcaaca gatagatcac
    14341 tgatctgcag tcctcgatcc gcaaaattcg ccccttcaac aaagtggaca ttgcttaaaa
    14401 tcgcttcatg attggcaaga taattcaaga tccaaaaaac ttctcttcgg ctaaaagata
    14461 catgattgac catccacgtt aaaaaattct tcttttcccg tacatcaatt agcatttttc
    14521 tcacctctct gttaggtcat ctaataatga cagtgcctcc atatctcctg gttcaagtgt
    14581 taaataataa gtcaataatt cttgcgctct tttcagctgc ccttcttctc ttaagaaaaa
    14641 ggcatactct ttcaagaaat ctggttcatg tgatagttca tgataagcct gttcatagtg
    14701 tacagctgca acggtaaagt cttctaattc attataagca tgggctaggt tccattctgc
    14761 ataaggatta gcagtttctt ccatctgatt gatcacttgt atcacatctt catatctttc
    14821 atcatccaag taaagattac ttaaagtaag taatgtttca tcttgtttgt ctcctacttc
    14881 taaagctttc agtaaaagtt cttctgctcg ctgtctatca tgcagacgga aagcattttc
    14941 cgaggccagg tgataaagtt cgacttggta aggattttct ttgattcctt cttctaacac
    15001 tgtttgtgct tcttcaatca tttcttcttc ttgaagagct tgtcctagat atagatataa
    15061 cgattgataa tgcggattaa cttctcgaag ttcttccaga tagttgattg cctgctgatt
    15121 ttctttcaat tgaagattcg taaaagctag ctggaacagt cgatcatctg tcctttcttc
    15181 tttcagtgct tcttctaaag gtacgatagc tgcttcgaat tctccttgca tacttaagga
    15241 ttgtcctaaa cgttcctgca tcgagattcc tgaaatttca tttatcccgc ttgccaacaa
    15301 tgtttcataa accactgctg cctctttgaa acgatcaact gaaaaataca attcagctag
    15361 cgcaaactgg atcaatggtt catcaggaag caacccagct gcttctttca attttgcttc
    15421 actcacttca gggattccga taacttggta caaatcagct atcgccaata agctttgcgg
    15481 atagtaatca ctttgcttat ccactttttc caggtattca aaagctaagt cgatctcatt
    15541 atcttcgatt gcaatttctg ctaacgggat gttcagtccg tcattttctg ggtattggtt
    15601 gatcagttgt tcaaaaatct gttttgcttc gatcaaaaag ccaatagcca gcaattcttc
    15661 gcctagttca gctaatatat ctgctgggtc ttcttttaat gctttttcta aaaagatttg
    15721 tgcttgtgac aagtcttcat ctgataaagc ttgcaacatt ttttcgctat ttgtattcat
    15781 ttcaattccc tcttatctca ctgacgattt gttgaaatac cgtcattgtt ctcaataatt
    15841 ctttttcgtt tggatgttgg tatccgtcta ttatacctat tttttctaaa acaagtattt
    15901 ttttgctttt ttctgccaaa aaaagaactt tttccaaata ctccatcttt gaaatctgtt
    15961 ccagccagtg gattggatag ccaagcaagt ccagccacac aaaaaagttt tttagttgga
    16021 aatcaaaagg atatgcttgg agattccaca cgaaatgaaa catcattcct aaaaattttt
    16081 tcatactagc agaaagtaga tggccatttt cggtcagata aaaagcttgt tcaaacagtt
    16141 taccataatt tgacaggttt tctgcctcta attgataaaa atagatcatt tcttcgatca
    16201 tcccggcaaa cggccgagtc gttaattgct ttttatttgg atagttttta tacagatttt
    16261 gcaaaaaacg atgatcacaa atcattccgc aacagataaa agtcaaaaag tcaatctgct
    16321 ttccgtccac ctgccgctcg ctgattgttt gatcataaac gatgcgttct ggcagattga
    16381 tggtttgcag cactacacga tttggtagct gcaaaatcgt ccgctgcggt gttaatgctc
    16441 ggcttagaga acggatcgat acagggatca tccatagtga tgaatcaagg gttgtatgtt
    16501 tttgaaaaaa acctgccaga tccatgatcc cttcatttcc aaatccgatg ataaacatgg
    16561 attgattttc gggatagcgt ttgacgaaat caagcaggtt ctttaattct gttaaatgat
    16621 tgcattgagt gttggcacaa atataccagt cattgtttgc ttgatttgca aataacgggt
    16681 tgatcttatc tgcaaacaaa tcataatagc gctgattcgt taaaaataag attggctgat
    16741 caatggaaag ttctcttagc tgactgacta gcgtttctcc gtaaaacact ttcgtcaacg
    16801 catcttctac ttgataatca agttccatac gcatccctcc tcttcttatt tttattttac
    16861 cattagtaag caaattctag aaaagataag agcaaagctc acctcataac ttgcaaataa
    16921 ataaaaatta aataaaatac atgtgttttc tatgtatgac atacggattc ggaatctctg
    16981 tctcttattt attccttttc tgctaggaaa taaaaagaaa agcacttgta gccctagagc
    17041 cacaagcgct ttttcaactg tcttatttaa cagcgtcttt taatgcttta cctggtttga
    17101 atgcaggtac tttgctagct ggaatttcga tttcttcacc agtttgtggg ttacgacctt
    17161 tgcgagctgc gcgttcgcga acttcaaagt taccaaaacc gattaattga actttttcgc
    17221 cgttagctaa agcatcttga atagttgaaa atacagcatc tactgctgca gttgcatctt
    17281 ttttagttaa atcagtagct gaagcaactt tttcgattaa ttctgctttg tttgccatgg
    17341 atagattcac ctcctccaca agagatatga tagaaatatc atttagttaa aactttctga
    17401 gtggtccaga agtttaaaaa gaaatattga ttaacttcaa tattctgtta tcacgatatc
    17461 atatgaaccg cttaatagca aggtttttac caattttcac atgctttttt tagtcataga
    17521 aacgtaacaa acgaaactgt ctttttcttt tgaaaaaaaa acgtcgatat ggtaaaatgc
    17581 ctattttctc ctacgaggga taattcggat ggacgtccct tcaaaagtaa atgcttttct
    17641 gatttgattt tctaagaaac gttcataaga aaaatgcatc aattcctctt cattgacaaa
    17701 aacgacaaat gtaggtggtt tgacagcgac ttgtgtagca taaaaaatct tcaggcgttt
    17761 tcctttatct gttggtgttg gattgattgc tacagcatcc ataatgatat cattcaacac
    17821 agccgaagag atacgaagat tctggttcat acttacttgt tcgatcattt caggcagacg
    17881 ttccaaccgt tgtttagtca ctgcagaaac aaaaacgatt ggagcgtaat caagataccg
    17941 gaattcatca cgaatctctt gttcgaaatc acgcattgta ttggtttctt tctttacaag
    18001 atcccattta ttcacgacga taataattcc acgaccagct tcatgggcgt aacccgctac
    18061 tcgcttgtct tgttcccgaa tcccttcttc agcattcagt accatcaaaa cgatatcaga
    18121 acggtcaatg gcacgcattg cacgcatcac gctgtatttt tcagtatttt cgtatacttt
    18181 tccgcgttta cgcataccag ctgtatctat cattaagaat ttctgaccac tttcagaaac
    18241 gaagtgggtg tcgattgcgt ctcgggtcgt tccttcaata tccgaaacaa ttacacgttc
    18301 ttctcctaaa atcgcattga tcaaagagga tttaccaaca tttgggcgac caatcaaact
    18361 aaacttaatc gtgtcgtcgt cctcttcttc agtctcttca gagaaatact tcactgcttc
    18421 atctaaaata tcaccaatcc ctagtccatg gcttcctgaa acaggataag gatcgcctag
    18481 ccctaaggaa taaaattcat agatatcgtt tctcatttct ggattatcta ctttattgac
    18541 tgctaaaatc actggtttat tacttctgta taaaattttc gcaaccatct catctgcgtc
    18601 tgtcacgcct tcgcgtccgc tgactacaca aatgattaca tctgcttctt caatcgcgat
    18661 ttctgcttga tgcttgatct gatccataaa aggttcatcg ccaagatcaa ttccgcctgt
    18721 gtcgatcacg ctgaattctc gacctaacca ttcacctttt gcataaatac gatcacgagt
    18781 tactccagga gtatcttcga caatcgagat acgctcacca gcgatgcggt taaaaatcgt
    18841 agacttaccg acgttcgggc ggccaacgat tgcaattgtt ggatttgcca tattatttcc
    18901 tcctcacaag tttccaatct cacttagttt aacgaatgca ttgccaactt tcaagcatag
    18961 attgtcaatt aaacgaagta tttttattcc ttattgaaat actaatcgct tttcaaaaga
    19021 aaaactgaga caaaagcagc tgctttcatc tcagtcacag atacaaaatc aattattttt
    19081 cagaatcatc ggttgctttt tcttctactt cttctttcaa cgcatcacct aaaatgtctc
    19141 ccatagtaaa tccagtattt tcttctggta attcataaga ctcaacattt tttggttctt
    19201 cttctgaagc tggtttttct tctaaagctt tgatactcaa tgcaatacga tgttcttctg
    19261 gatggatctc taatactttc actttcactt cgtcaccttc atgaagcact tcatgtggtg
    19321 tcgcaatatg tttatgtgaa atttgagaga tatggactag cccttctact ccagggaaca
    19381 cttcaacaaa ggcaccgaag cttgtcaaac gtttaactgt tccgtctaat actgtgccaa
    19441 ctggagcttt tgtatcgatt tctgaccaag gtccaggctg tgtgtctttg attgacaagg
    19501 aaacacgttc ttgttcagga ttgatagaca acactttgac atttacatca tcattgacag
    19561 ccaatacatc acttggtttt gctacatgac tatgtgaaat ctctgagaca tggactagac
    19621 cgtcaattcc gcctaggtcg acaaatgcgc caaaatcagt aagacgtgca acacgtccac
    19681 tgagaacatc tccctcatgg atagttgaaa gaatttcttt tttcttttct tctctttcgt
    19741 tttccactac cgctttatgc gataagatca aacgattttc tgatggttcg atttcgatga
    19801 ttttaaagct caatgttttg cctttatagt ctgaaaagtc tgcaacaaaa tgatcttcca
    19861 ccattgatgc agggacaaaa ccacgtacac cgacgtcaac gactaaaccg cctttgacaa
    19921 catttgtaac aggtgcttcg atgaccttgc cctcttggaa atctctttcg atatcttccc
    19981 aaactttttt agcatctaaa cggcgttttg aaagtagata gctgccgttt tctttatctt
    20041 tgccgatcgt actgataacg actaaatcta atacatcacc gacttttact acttcgctga
    20101 tatcttctac cggcaaagtt gataattctt ttgccggaac gactccttca actccagccc
    20161 cttcgattcc gacaatcact tgtttgtctt caatgactaa aacttcgccc ttaacaacat
    20221 cgccaacttt tacttcttgt acgcttttca ttgcgtcttc cattgactgg ttgttctctt
    20281 gattttgatc aaattctgtc ataacacttg tcctcctaac caacattctg ccgtaagtac
    20341 ggtactgttt ttagtttaat tgattattca aaaaaaataa agcgatttcg ttcgttttcc
    20401 ttgaaaaaac aattgttttt catttttttt cttaatttaa aaaagaagat aacctttttt
    20461 caaaacaaca gcttttattg ctgcaacgac ttctggaatc gatttgccag tcgtatcgat
    20521 acgaacagca tcctctgctt gcttcagcgg agagacgtcc cttgttgaat cataatggtc
    20581 tctttcctgt atctctttcg tcagtgtttc aagatcagtc ggaattcctt tttcttggtt
    20641 ctctttgaaa cgtctttctg cacgctcact tacgcttgct actaaaaaga ttttcacttc
    20701 tgcattcggt aatacagctg tcccgatgtc tcttccgtcc atgacaacgc caccgcgttc
    20761 ccctattttt cgttggacag caactaattc ttctctgact tttccatgtg cagagacttc
    20821 agaaacagct tttgtgacat ctggttgacg gatttctaaa gtcacatctt ttgatgctat
    20881 gaatacatgc tgtccatctt cttgttgcga aaaagagata ggatactttt tgatcagcga
    20941 aactagccct tcttcatctg aaaaagatac attgttcgta atagctaaat aagttactgc
    21001 gcgatacatg gcaccggtat ctgtataaat ataattcaat tcttttgcta atatcttggc
    21061 aacggtactt ttcccagatg aagcaggccc gtcgattgca atactgattt gtgtcatgat
    21121 ataactcctt tttctcagaa aaaaagccgc aaagcgcggc tattattatt taattcttaa
    21181 ttcttgacca gggtaaagca taaagttgtt tggatctaag ccattcaatt caaacaattg
    21241 atcagtggtg atccctgcac gctcagccac ttgttttgga ccttcgcctg cttgaacagt
    21301 cgttgttgaa ccggctgcag ctgatgaaga aggtgttgtt tctgctgcat tgctgcttga
    21361 atcccctgct tgataagttg cctcacttga ggaagtcggt gttgtctctg ctgcaatact
    21421 gctttctgac gttacacctg tatcttgaga cacagtagat tcttccgcga ttgtactaga
    21481 ttctactgtt gaagtctcag ttgactcctc ttttgttgta cttgtagaac tttctactac
    21541 tgatgtcgaa gtttcagaag aagttgctgc tgttgcattg ttgcttgatc catttctgat
    21601 ccagaaataa gcacctgctg gaatagcgat acataagacc agtaaaatca aaaatagcga
    21661 aagaaataat gtatttcctt tcttttgttg tctctgagaa cttcttgatg aaacttcatc
    21721 atctgtatcg tagattggtt gttcccatgg ttcttgtgtt tcgttatttt ctgtagattg
    21781 atgtctatct tttctgctca ctgttttacc tcctaaactc tatcgtgtgt attataccat
    21841 aggaaaaagt gtcttgtcct aaaaaatcaa tgttccatgt tttttttaaa taaatttagt
    21901 aaaatcgttc cccaatgaag ttgttcagtc ttagaaaaaa gcttcctctt tggcagaagt
    21961 tctgcttttg ctccatcaat atcacaacaa ttctgcggct tttcggagag tttttcgcca
    22021 aaataagcta agatgaattt tctgcggcag ttttcttcat taatatagcg aagcatctgc
    22081 tgcaggcgga attctttttc tttttcctga cgtttgagcc gttccaccca attttctggt
    22141 ttttcagact gctggatcag ctccatccat tttttttgca gctcatcaaa gggagcttgc
    22201 tctgtggcat attccaaata aagctcaaaa ctttgtcgct gttctctaga aagttgatta
    22261 aagaaataat ggatacgttc atccccactt tgataaaaaa gaattgctgc actttcttcc
    22321 tgatctctgc ctgctcgacc gatttcttgt acatagtttt ctagactgtc tggcaaatca
    22381 taatggacca cataacggat atcagattta tcgatcccca taccaaatgc attcgtagca
    22441 atgagaaact gcagctgatt ttttacaaac tgctgttgaa gttgtcttct ttgagctgca
    22501 tcaagaccac cgtgatagta tccaactgta aaacgttccc tgaataaatg atacaatcgt
    22561 tcgacttctt tttttgttgc acaataaatg attgccgctc cttgtgctct ttccatgaag
    22621 tgttcaagtt cttgttcttt ttgctgcgtt ttccgaacaa aaagcgagat attttgccga
    22681 ttgactgatt gtctgatctc ttttggtggt cgatcaaata gtacatgacg tatatctctt
    22741 gctactaaat ctgttgctgt cgccgttagt gccagtgtga ccgggtttcc taaaagcttt
    22801 cggatttttc ctaactgttg atattctgga cggaaatcaa ctccccattg agacacgcaa
    22861 tgagcctcat ctactacata taacgcaata ttttgttttt tcagatgctc taaaagagaa
    22921 ggattagtca acatttcggg actcaaaaac aaaaatttat attgcgatag atgacggagg
    22981 acgtatcttc tttcagattg cgaaagtaaa ctattgaaag cagctactcg tttctcccct
    23041 cgtttttgta attgcgtcac ttgatcttcc attagagaaa gcagcggtga cacgatgatg
    23101 accagacctt ccattaaata ccccgtcaat tgatagcaaa ggctttttcc cgttcctgta
    23161 ggcaaaatag cgagagtatc ttctccatca agcaacgcct gaataatttc ttcttggccg
    23221 ggacgaaatg aatcaaatcc aaaatatttt tttaattcat ttattaacat tctgtatcgc
    23281 ttcctttctc aatagataga tttgatacaa acgaaattct ccataatcta attgtccatc
    23341 tacattccat tcgctaaagc gccactctcg cacggaatcc ttatttggtt ccaaacgttc
    23401 gattgtttct gcagagagaa tccactcgta tggaaaatca tcaaaaaaca aggcccactc
    23461 aataagatga tctgttactg ttcctctttt taaatgacgg attgccatga tctggtcaat
    23521 tgtctgacca ttcataaaca tctgcttggt gatcatcatg ctttgattga aattttgctg
    23581 taataatgaa tcgatcaaag cgtgccaaag cgaatctggc cgtttttcga tttgtgctaa
    23641 caaaaggtgg atacatctgc tttgttgcag ataaaccgct gactcatcat tgttttttgc
    23701 cagctgataa ggaagcagcc ctgttcgctc atttccagat aattggttag ctaaaaaatc
    23761 tgctgcttct tctggtatct ccgaaaacag ctgcaccaaa ctctcatacg cagtatcaat
    23821 caaaatttca cgtggcaatt ggctttcact aagccatttt tttagttgga aagtataaaa
    23881 aggacttgtc tctgctggaa gatagtcttg aatacccgtt gccagattag aaattacttg
    23941 aacagcaaat ttaatgagcc gccaactagt ttcccctgtt ctgccatagc ggtcaaaacg
    24001 caaaccagta tgttctattt gatgttcgga caacatatca gcccctgcag gcgttagctt
    24061 tgcttcattt tcatttattt cgatccaacc ttgctgcgca attttttgca aaatttgata
    24121 aaacttatcc tgcttcaatt ctggaaaact gttatggata aacaaaagtt cgtgggtaaa
    24181 tccataaata agtactgaag tcgttcgttt tcccacaagg agttggtaaa gagaactaat
    24241 ccgcagcttg tgtcctgatg aaaatagcga taaaataaaa acaatcatat tattctcctt
    24301 aagttaggat ggttagaaat gtcactacta tgtaaacttg tacctgaacg ctgtattgca
    24361 tgcggtcttt gtcaaatcga agcgccagat attttcgatt atgatgaaga aggaatcgtt
    24421 ttatttgccc aagaaccaga ggctcatcaa cagttcgtga tgccggaaga agaaacatat
    24481 gtaaaaaaag cgtatcaaaa atgtccagtt cgagcgattt tattagaaaa taaaatataa
    24541 atataaacga aactttaaaa acgtacacta agttctctaa acttgaaaat aagccgggat
    24601 ctgtttctct tgttccgtga aatcccgact tattttgaaa gattcatttg ctatctatag
    24661 ttaattgact agttttttca cggaccctat aggacccatt tatattattt caactttttc
    24721 agccagtttt ccaatgtatt gacgatcaca gtttgctggt ctgcgttaga aatttctgcc
    24781 tctccatctc ctttttgctc accatagctg ccaaaacctc catgatttcc gccattgatt
    24841 gatacatact ctgttctttc cggtaaatac tgcttagctt cttgataagc ttcctgattc
    24901 aatactttat cttttgtcgc cgtgatcgat aaaacaggaa cagctgattc tgtcaatgtt
    24961 ccttttttgt cgggataact tgccatgaag aatacccctt ttaaatgttt gtctgaatga
    25021 ttcttattga cataacgact tgccattacg ccacctaatg aatggccacc gatcacataa
    25081 tcggaatttg gaaaatcttc tttgacctta tccgctttgt ttccgcccat cactgctaga
    25141 tcaagtggca ttttgacaat agcgactgaa aagccgtttt ttgctaaatc ttgcgcccaa
    25201 atactataac tttctggctc tacgagagca ccaggataaa aaataagtaa tggagacttt
    25261 ccatgattct ttttctctcc ttcaaaaaat aagtaatctt ctttgttttt tccttctttt
    25321 accaacactt gcgcttctga cgatggttcg tacgtatttt gctgtatata gtaataaccg
    25381 gctccaccga gtaatcctaa aatgatgact aagccaatta gtattcgtct aaatattttc
    25441 actttctccc accttttcta tctgagtgac cccttttt
//
LOCUS       DAYYCW010000030        25334 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000030 DAYYCW010000000
VERSION     DAYYCW010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.030"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..398)
                     /locus_tag="ACHAYA_001155"
     CDS             complement(33..398)
                     /locus_tag="ACHAYA_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604967.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            606..1088
                     /locus_tag="ACHAYA_001156"
     CDS             606..1088
                     /locus_tag="ACHAYA_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604968.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1285..2175
                     /locus_tag="ACHAYA_001157"
     CDS             1285..2175
                     /locus_tag="ACHAYA_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFM8604969.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2501..4975
                     /locus_tag="ACHAYA_001158"
     CDS             2501..4975
                     /locus_tag="ACHAYA_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8604970.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5078..6814)
                     /locus_tag="ACHAYA_001159"
     CDS             complement(5078..6814)
                     /locus_tag="ACHAYA_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8604971.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6811..7515)
                     /locus_tag="ACHAYA_001160"
     CDS             complement(6811..7515)
                     /locus_tag="ACHAYA_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8604972.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7646..8149
                     /locus_tag="ACHAYA_001161"
     CDS             7646..8149
                     /locus_tag="ACHAYA_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM8604973.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8109..8447
                     /locus_tag="ACHAYA_001162"
     CDS             8109..8447
                     /locus_tag="ACHAYA_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM8604974.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8468..9886
                     /gene="ffh"
                     /locus_tag="ACHAYA_001163"
     CDS             8468..9886
                     /gene="ffh"
                     /locus_tag="ACHAYA_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM8604975.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9998..10576
                     /locus_tag="ACHAYA_001164"
     CDS             9998..10576
                     /locus_tag="ACHAYA_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM8604976.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10580..11101
                     /locus_tag="ACHAYA_001165"
     CDS             10580..11101
                     /locus_tag="ACHAYA_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM8604977.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11180..11734)
                     /locus_tag="ACHAYA_001166"
     CDS             complement(11180..11734)
                     /locus_tag="ACHAYA_001166"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8604978.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11987..12262
                     /gene="rpsP"
                     /locus_tag="ACHAYA_001167"
     CDS             11987..12262
                     /gene="rpsP"
                     /locus_tag="ACHAYA_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM8604979.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12274..12525
                     /locus_tag="ACHAYA_001168"
     CDS             12274..12525
                     /locus_tag="ACHAYA_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM8604980.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12649..13440
                     /locus_tag="ACHAYA_001169"
     CDS             12649..13440
                     /locus_tag="ACHAYA_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604981.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13610..14131
                     /gene="rimM"
                     /locus_tag="ACHAYA_001170"
     CDS             13610..14131
                     /gene="rimM"
                     /locus_tag="ACHAYA_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM8604982.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14121..14882
                     /gene="trmD"
                     /locus_tag="ACHAYA_001171"
     CDS             14121..14882
                     /gene="trmD"
                     /locus_tag="ACHAYA_001171"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM8604983.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15018..15365
                     /gene="rplS"
                     /locus_tag="ACHAYA_001172"
     CDS             15018..15365
                     /gene="rplS"
                     /locus_tag="ACHAYA_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM8604984.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            15675..15917
                     /locus_tag="ACHAYA_001173"
     CDS             15675..15917
                     /locus_tag="ACHAYA_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM8604985.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(16005..16151)
                     /locus_tag="ACHAYA_001174"
                     /pseudo
     CDS             complement(16005..16151)
                     /locus_tag="ACHAYA_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            16208..16522
                     /locus_tag="ACHAYA_001175"
                     /pseudo
     CDS             16208..16522
                     /locus_tag="ACHAYA_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            16551..17810
                     /locus_tag="ACHAYA_001176"
     CDS             16551..17810
                     /locus_tag="ACHAYA_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8604986.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKVVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            17863..18759
                     /locus_tag="ACHAYA_001177"
     CDS             17863..18759
                     /locus_tag="ACHAYA_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8604987.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            18770..19639
                     /locus_tag="ACHAYA_001178"
     CDS             18770..19639
                     /locus_tag="ACHAYA_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8604988.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            19649..20324
                     /locus_tag="ACHAYA_001179"
                     /pseudo
     CDS             19649..20324
                     /locus_tag="ACHAYA_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            20530..22194
                     /gene="alsS"
                     /locus_tag="ACHAYA_001180"
     CDS             20530..22194
                     /gene="alsS"
                     /locus_tag="ACHAYA_001180"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM8604989.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            22207..22917
                     /gene="budA"
                     /locus_tag="ACHAYA_001181"
     CDS             22207..22917
                     /gene="budA"
                     /locus_tag="ACHAYA_001181"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM8604990.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            23047..23544
                     /locus_tag="ACHAYA_001182"
     CDS             23047..23544
                     /locus_tag="ACHAYA_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM8604991.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            23729..23989
                     /locus_tag="ACHAYA_001183"
     CDS             23729..23989
                     /locus_tag="ACHAYA_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604992.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(24351..24590)
                     /locus_tag="ACHAYA_001184"
     CDS             complement(24351..24590)
                     /locus_tag="ACHAYA_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604993.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            24876..25190
                     /locus_tag="ACHAYA_001185"
     CDS             24876..25190
                     /locus_tag="ACHAYA_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8604994.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT         8392 a         4298 c         5212 g         7432 t
ORIGIN      
        1 gtagcttctc tttgtacata tatggtttgt ttttatggat gccgagcttt tttataagat
       61 aaggctaccg cccataacga cccaaggatt tgtggaacta agtaaatacc tgcaagaagc
      121 aagatcagct gggtcggttt taaaggatga tagaagtaac gccattcagt aaaattggcg
      181 caaaataaat aggaagtgac agtagatata atattggcaa tgaataaaga tataatacgc
      241 ctgttccttt gaaaataata tccagcaaaa agagataaaa atatcaatgt gatgaacccg
      301 cttacggaat gataacgaaa atcaagcagt aaggcgaaat gcacataagg aacaaaagat
      361 aaaagcaacg gaaggttttt ccagcaagtt tttatcatct ttcatcacca cctaacaagt
      421 gttattattt tgttagtttt attgtaaatt gaatcctgca ataatcaatt actttttcat
      481 tatttctaga ttaaatgaat aaactctcat gccattatct ctaaaaaaaa agtttttccc
      541 cctaattttt ttagatagaa aattgtatag tatgatgaga gaaaaagtag tttgggggaa
      601 cgttaatgag aagaaaagca gtaatcgatc ggctttattt atttttttgg ttttttccta
      661 tcgttttgct accttttaca tgggtaaatg ctgttgtaca aggtgcggag atattaggcc
      721 tgatccaagg aagcggatta gtggtttcta ggaattttcc agctttgact gttttctatt
      781 tagtgataag tgtaggtctc ttattaccaa aatctctgat caatcactac aattggcagc
      841 tgctttactt ttcttgctta ttagtattta catctatgtt ttctttgctg cctcgcttga
      901 ttatcggccc taatggtttg agcgagagtg gattgaagga ttcagcttat tcctccatgt
      961 atttcttgac gatcaaacca tggttctatt tagtaattgc ctcatttatt cttgcaacat
     1021 ttttttactt ctggacagaa atcaggataa aaagtgaacg atctgtatct ataaaaaata
     1081 actattgatg gtggatcatc agtaaaaaag catatgagct gcgtaaaatg catgtttctc
     1141 atatgctttt tttattatta tgtaaagaat gcgaaaaacg tttacatgat ttttacataa
     1201 cttaactata tttttacagc aaccatacga gggatttaca gtcgactgat aaactttagt
     1261 tgtaaataag aaaggagcaa taaaatgaaa aaagtattgt ttactctagg tatgatggga
     1321 attcttttag ccggatgcgg ctcaggatca ggaaatacag atggctctgc cacacaaaat
     1381 aattcaagtg actcaaatga acaagtaaaa atcgttgcgg taggttcgac agcattacag
     1441 ccgttagtcg atgcagcaca agaaagcttt gtacaagaaa atccaaatta tcaaatctct
     1501 gtacaaggag gaggaagcgg aactggtttg agccaagtcg aagctggagc agttactatc
     1561 ggaaactctg atgtatttgc agaagaaaga gacggagttg atgcttcaaa attagtggat
     1621 cataaagtag cagtggtcgg gatggcacca atcgtcaata aagatacaga tgtcaaagat
     1681 atcaccaaac aggaattgat tgatatcttt acaggaaaaa tcactaactg gaaggaagtt
     1741 ggcgggaaag atcaaaaaat caacgtcgtg aaccgtgcaa acggcagcgg tacccgcgcg
     1801 acattcgaaa aatggggact ggatggtgct acccctgtgc agtcccaaga acaagattca
     1861 tcaggaacag ttcggcagct ggtaagtcaa acaccaggag caatcagcta tttagctttc
     1921 tcatatctcg atgattccac acaagcacta agtatcgacg gtgtagaacc aaaagaagag
     1981 aatgtagcag ataacagctg gggaatctgg tcatatgaac atatgtacac gaacggaaaa
     2041 ccttcccctg aagtccaaaa attcttagac tacatgatga cagaagaaat ccaagaagga
     2101 cctgtcaaag aattgggcta tttaccaatc acaatgatgg aagtagaacg tgatcacgaa
     2161 ggaaatatca aataactagc agataaacat gccacggtag tcgttactat taaggaaaaa
     2221 tcaactatgt ttccccacat atacattttt cctttaattg tgaaaaatag ttcctcatat
     2281 tttgccgttt tttgagctaa taccgagggt cagctgattg aacaccgttt attcgaaaaa
     2341 aagagctacg acatagaact gtcgtagctt tttttttgct atatgacagg aatgaaaggt
     2401 gtttgcaagc ttaatgtgag gaacactttc catatctgtt ctatactgga taggagaagt
     2461 ttggaaatat ccagaaaacg aaatcaatcg aggtagccac atgaagaata aatggataaa
     2521 tcgaagtaaa aaaaagaaag taacacaaag atcatcagat aaagtcatcc gtctttttcc
     2581 atcgtataaa gaaggattaa cggcagatca agtcaaggaa cgaatagaaa aaggtgcagc
     2641 aaataattca gtcgatccca cattcaaaac aaatcagcaa atcgtattgg aaaacatatt
     2701 tacgtatttc aatttgatct ttcttatctt agccattttg ctttgtttag tagaatccta
     2761 taagaacttg acttttttac cagttatcat agccaataca ggaatcgcca tttttcagga
     2821 aattcgatcc aaaaagatct tggacgaact aaatgtttta aatacagcaa aagtcaatgt
     2881 catacgagac gggatagaac aaacaataga tatagaagac cttgtaatag atgacattgt
     2941 tattttagag actggacatc aaattccagc agatgcagag gtcgtggaag ggaaactgca
     3001 agttaatgag gctttattga caggcgaagc agatgaaata accaaagaaa taggcgatcc
     3061 attaatgtcc ggcagtttca ttgtttccgg aaaagcttac gcccgattag acaaagttgg
     3121 agcagattcg tatatttccc agctgacagt caaagccaag acaatgggag aaggagaaca
     3181 gtcggagatg attgcttctc ttaatcaatt gatcaaatgg attgggatca tcattattcc
     3241 tatcggtatc ctgctttttt ctcaaagtta cttttttaat ggaaacacga tcaaagaaag
     3301 tgtcgttgca atggaagcag cattgatcgg tatgatacct gaaggactgt acttgctgac
     3361 gacgattgca ttagcattaa gtgcgactaa actagcaaag caaagagttc ttttacacaa
     3421 tatgaaaagc attgagacat tggcaagggt aaatgtcctt tgtgtagata aaacaggaac
     3481 gatcacagaa aataaaatgt ctgttcaaaa agtgatcgtc tctaaaaaac aagaaataat
     3541 cagtcagtca gatcaattgg aagcatgcat tgctgactat gcaaaagcga tggcaggaga
     3601 caatgcaaca atggaagctg ttaaagcata ttttacaaaa accactgatc aatcctatca
     3661 ccatgttatt cctttttctt ctgtccaaaa attcagcagt gtagcgttgg cagacaaggt
     3721 gtatgtttta ggagcgcccg aaatggtttt gcgcgatcaa ttgccggaat atgcggaaga
     3781 gtttgtttct ttcgctgaaa aaggctatcg cgtgttggtc tttggtgtgt acgatgggat
     3841 tcttgaagaa ccagtattga cagaagcagt cacaccatta ggctatattc tgattgctaa
     3901 tcctatcaga aaagaagcaa gagccacatt tgattacttc aaaaaacagc aagtagccat
     3961 caaagtgatt tctggagata atccgctcac cgtatccaat gtagctgtcc aagcagggat
     4021 ttcacatgct gatcagtacg ttgatgcatc taaattatca gaagaagaat atgaagcagc
     4081 tgtcgaaaaa tatacagttt ttggacgcgt caagccagaa caaaagaaaa tcttcgttca
     4141 attgctaaag cgtcgtaaca atacagtagc tatgacaggt gatggcgtga atgatattct
     4201 agccatgaaa gaagcagact gcagtatagc gatggcttcc ggaaatgaag ccgccatgca
     4261 ggcctcacaa gtggtcttac ttgattctga cttttcacgc atgccagaag ttgttgcaga
     4321 aggacggcgc gttgtgaaca atattgagcg atcagccagc ttgtttttag tgaagaatat
     4381 tttctccttt ttgctctcac tgttttcagt gatctttgct ctgacctatc ctttagaacc
     4441 atcacaaatc acattgatta gtttgtttac tatcggactt ccatctttct tgcttgctct
     4501 agaagaaaac aaaaagcgaa tcagagggaa attcatcatg aatgtgatgg aaaaagctgt
     4561 gccaggtgga ctaacagata tgatcgtagt cggtgcgttg gtgatctgcg gggtgacttt
     4621 gaatctaaat aaaacagatg tttctacagc atctaccatg cttttgattg cagtagggtt
     4681 cctcgttctc tataaaatct gttcaccgct gaataaattc agaagtcaga tcattctttt
     4741 ctgtgctagc ggtaactttt tttcggtcat ctttttgcat aagctgtttt caatcaccgg
     4801 tatctcagct gtatcacttt tgctgttgag cattctattt ttctcggcag attcgatttt
     4861 taggcatttg accacattgg tagaatattt gtttgaacgt aaaaatgaaa aagagcaaaa
     4921 gccgtttagt cgcgtagtta aaaaatttct atcgatattt aaacgagaaa attgacgaaa
     4981 agtatctcaa aaaagagtaa aaaagaacaa ccactcgtgt atctttcgaa gcaggatgag
     5041 tcatccaact ttaaaacgca cgagcagttg ctctttttta cattttcgga atggtaacgg
     5101 tgaaagtcga acctgttcca agatgactgt ctacagtaat cgtaccatga aggagttgca
     5161 cgtagtcttt tacaatcgct aaccctaatc cggtcccgcc tgaatgccgg cttcgcgcct
     5221 tgtctacgcg gtaaaaacgc tcaaagatcc gttcctgatc ctcaatgtcg atacctatac
     5281 caaagtcttg gacagaaaac gatagatcat ctgttgcctg atagcggata ataatctcgc
     5341 tgtcgctttt tgaatattgg acagcatttt caatcagatt tttgataatt ggataaagga
     5401 tatctgtttt agtagtaaat ggttcgttct ttccaatcaa tcgaatcgtc aaccgtttct
     5461 tttcgatgat tggctgataa ttttgaacga tttgctggaa ataaggttct gtataaattg
     5521 tctggatctc gtaagaattc tcgccatctt ttgacagctg gatgatttcc cgaatcagtt
     5581 tatctaaccg gatcgcatct ttttgcatga tctcaagaaa agaagtcaga gtttgcgggt
     5641 catcttttgc accatccagt aaagtctcgg taaaaccaat cagtgaagtc acaggagttt
     5701 ttaattcgtg agagacgttg ccaacgaaat ccttttgtag cttttctaaa cgtcgcactt
     5761 tcgttaagtc atacgccacc cctagaatct gtccggtacc ttctgaatta gtaaagtagc
     5821 gcaaactcaa gtctaatgtc tgccgacctt ctgtaagggt gatctcctga tggatcagcg
     5881 gatgctcagg agtcacttga tgaatcagct gaatgagttt tggctcttga atgacttctg
     5941 tgtaccgctg ttcaggttga tagcttacca cgtttaaatg tagctgcatc tttggattaa
     6001 gcagtagtaa acgagaatca gcatcatcaa tcaggaatac gccaatcatc agttcgttca
     6061 gtaacgtata taattgctct tctgtggtag tatacgcccg atacatctta ctcatttgtt
     6121 cgctcaacgc attgatcgtc tgatacaatt cttcccattg atcgggtgtt tgcatgatta
     6181 cttccgcctg attaggattt tgcaccattt tttttagtac gggcagcacg gttttcaatg
     6241 gctggttttt ttgatagatc agataataga tcattcctgc aagaacaagg aagaaagaca
     6301 gaaagaagaa aaagacccaa cggcgaaaac tctcagttct cggtaaaaaa ccgcttgtcg
     6361 gttctgcgat acgtaaaatg gcttctaact ggccactttt tttgactggt aatgcgacat
     6421 ataaaagatc cccattcaaa gtagtacttt tgcgcaaagc gctacctagg ttcccaccat
     6481 tcaatacggc tttgatttcg ggacgggagt ttcgctgttc attcaatgtt tcattgtttg
     6541 tgtcaaataa gatctttcca gtagcatcca ttaaagtaat ccgttcgttc gactgatgga
     6601 cgaattcttc caaagcagtt ttgttttgcg ctgcttggag gttttcgaca tctaattgcc
     6661 ggatcagcag ttgtgctttt tttgtcaggt agctttcttg ctgttccacg atctgctgtt
     6721 gaaaataatg ggaaatcaat tgccagccac caaaaaacaa actaactaaa agaataagaa
     6781 agaagagacg ttcataaatt tttttccatt tcatcgtttg ggttcctgaa acttatagcc
     6841 aaatcctcta atagtcacca agtactttgg gtgttttgga tctcgctcga ttttttctcg
     6901 caaatgacta acgtgtacat ctacgattcg gctttgtcct gcgaagtcaa aattccaaat
     6961 gcggtctagc aatgtgtctc ggtcgatcac acggtctttt cgtttcatga aataaacaag
     7021 taattcaaat tctttcggtg ttagttcaat cagatggtcg tctacggtca cttgataatt
     7081 ggtcaaatca gcagtgattt gtccaatcga tagatattca ggctcagctt catcttttga
     7141 ttgcttacga ggttcaattc gtctgaatat tgctttcata cgggctaata cttcgcgagg
     7201 actaaatggt ttggtcaaat agtcgtcagc acctatttct aggccaatga tccgatccac
     7261 ttggtcatct ttagccgtca gcatcaaaat cggtgtttca tttttttctt gacgcagccg
     7321 ctgagtgatc tccatgccat ccatcgttgg aagcatcaca tctaaaatga tgaaatcaaa
     7381 tgattgttct aatgctaatt ctaatccttc gcctccatct gtggcactgg ttaccttgta
     7441 gccttctttt tccaaattga acgtcaagag cgttacaata gaaggttcat catcaacaac
     7501 tagtattttt ttcattcaaa tctcctttaa ataggtttca ttctgtttat attttagcat
     7561 agaatcagta caaagggcta ttttacaaaa gaaataaatg gtataatagg aaggcacttt
     7621 gctgaataga aaggaagagc agtcaatgat tggaatcagt gcatgtttag gcggtctggc
     7681 ctgtcgctat gatggacaga aaaaagaagt accagaattg atggaactag ttgaaactgg
     7741 agaagctgtc atggtctgtc cagaagtgat cggcggatta ccaattccta gagatcccgc
     7801 tgaaatcatt ggtggaaatg gattcgatgt gtggaaagat caagcaaaag tatggacgac
     7861 aagcggcgaa gatgtaacgg aagcgtacaa aaatggcgcg atcaaagcct atcaaaaatt
     7921 gcaggaaaaa cacattgatc tagtcatcat gaaggaaaaa agcccttcat gcggaaccca
     7981 ttcgatttat gacgggagtt tttccggaat aaaaaaaaat ggtgccggag tggctactgc
     8041 ttattttatt cagcaaaaaa tgactgtgct ctctgatgaa gaatggctga aaactagagg
     8101 tgagcagaat ggaaatcgaa aagaccaatc gaatgaacgc gctctttgaa ttttattcga
     8161 ccttattgac agaaaaacaa atgaactaca tggaattata ttatgcagat gatttttcat
     8221 taggtgagat cgcggaagaa tacgaagtaa gccgacaagc agtatatgac aatatcaagc
     8281 ggacaagcaa gatcttggag gattatgaaa aaaaacttca tcttttttct aattatattg
     8341 ttcgagaaca ggtattggaa aatatgactg catatattac agataagtac ccggaagata
     8401 aaaaactact tgaatatgta cagcaaattc aagcaatcga agaataaata aaaggaatga
     8461 agaaaatatg gcatttgaaa gcttaactga acgtctccag caggcgatgg gaaaaataag
     8521 aaaaaaagga aaagtatcag aagctgacgt aaaagaaatg atgcgggaaa tccgccttgc
     8581 cttactagaa gccgatgtta acttgcaagt agtcaaagat tttactaagc gcgtacgcga
     8641 acgtgcggta ggggcagaag tgctggaaag tttatcacct gcacaacaaa tcgtaaaaat
     8701 cgttgatgaa gaattgacga tcacacttgg ttctgaaaca gcagagttaa ataaatcacc
     8761 aaaaatacca actgttatca tgatggctgg tcttcaaggg gcaggtaaaa cgacctttac
     8821 tggtaaatta gccaattatt taaagaaaaa tgaaaatgct cgtccgctcc taatcgcagg
     8881 ggacgtatat cgaccagctg cgatcgacca attgaaagta ttaggtcaac agctagacgt
     8941 tcctgtattt gatatgggaa ctgaagtcag cccggtagag atcgtccgtc aagggatgga
     9001 gttagcaaaa gaaaagaaaa acgactatgt attgatcgat acggcaggac gtctgcatat
     9061 cgatgaaaca ttgatggatg agttgaaaca aatcaaagaa ttgacgcaac caaatgagat
     9121 tttgcttgtt gtcgatgcca tgactggtca agatgccgtc aacgtagctg atagcttcaa
     9181 ccagcaatta ggtattacag gggtcgtcat cactaaactt gatggggata ctcgaggcgg
     9241 ggcagcacta tcgatccgct cagtgacagg cgcaccaatc aaattcatcg gttctggtga
     9301 aaaattgact gacttagaag tattccaccc agatcgtatg gctagccgta tccttggtat
     9361 gggggatatg ctgacattga ttgaaaaagc gcagcaagac tatgacgaga aaaaagcgga
     9421 agaacttgca cagaaaatgc gggaaaattc attcgatttc aatgacttta ttgaacagct
     9481 tgatcaagtg atgggtatgg gcccactgga agatctaatc aaaatgatcc caggaatgaa
     9541 tcaagtccct ggaatcgaaa acgtcaaggt agatccaaaa gatgttgaac gcaaaaaagc
     9601 gatggtttat tctatgacgc cagcagaaag agcaaatcct gatctgttga acccaagccg
     9661 tcgtcgccga attgctgccg gttctggaaa cagtgttgtt gaagtgaatc gaatgatcaa
     9721 acaattcaag gaatcaagaa aaatgatgca gcaaatgtcc aaaggggata tgaacatccc
     9781 aggtatggac caaatgtttg gttcaggcgt caaaggaaaa ctaggcaaaa tggcgatgaa
     9841 tcgaatgatc aagaaaaata agaagaaaaa gaaaaaacgg aaataagatg aaaaaaacag
     9901 cttcggtaca gactgaaaat gtctactgcc ggagcttttc tatcagcaat ttttattctt
     9961 tactaataca attactctaa acgaagaggt ggcacagatg agatgtccgt ggagtgaaca
    10021 aacagaaagt atgcggcaat atcatgatac tgtctggggt gttcctgaat atgatgacca
    10081 aaagctgttc cgaaaattga tgcttgatat taatcaagcc ggattgagct ggcagacgat
    10141 tttaaataaa agtgaagcat tcgatgaagc atatgatcga ttcgatatta aaaaagtcgc
    10201 aaattatgga gaaacaaaaa tcagtgaact aatggaaaac aaaggaatta ttcgtaaccg
    10261 aagaaaaatc gaagcagccg tccaaaacgc caaagcagtc ttgaaaattc aagaagaaat
    10321 cggcagtttt tcagagtatc tttggtcgtt ttctaatgga aaaatcgttg attcttctta
    10381 tcattcacaa gaagaagtcc caacaacaag tcctctttca gatgaattag caaaagatct
    10441 aaagaaaaga ggatttaaat ttatcggaag cacaacaatc tatgcttttt tagaagctgt
    10501 cgggatcatc aatgatcacc ttgacagttg tttccgcaaa gaagaggtcg tacgtatcgg
    10561 cagaaagggc gaataaagca tgcgaaaaat catattttat ggtgctataa gtttagatgg
    10621 ctatttggca acgaaaacgg atgatcttca atggcttttt gacactccaa caggtaagca
    10681 aaccacttat gaagcttttt atgaaacaat cgatacgacc atcatgggca gaaaaactta
    10741 tcaggaagca aaaaaatatt tggagaccga agcaatttat ccagagaaaa agaatttcgt
    10801 tttttccacc cacgcacaat tacaattgcc agatgctgag gtcgtaaacg aagatcctgt
    10861 tgcttttcta aaaaatctac aaatgacaga aggaaaagat atctgggttg tgggtggcgg
    10921 tcgattgatc aaaccgctat tggaacatca aatgattgat gaatggtata tccagattac
    10981 ccctgtatta ttaggggacg ggattccttt attccaaaaa ggaacatatg aagcgcgttt
    11041 tgaaatgcta gacacgacac gattcggtga atttatcgaa ttgcattatg tacgaaagta
    11101 gaagtaaata aaataaaaaa ggcaactata tgcagaaatg tcctgtacat agttgccttt
    11161 tttattaccg ttatgctttt tactctgtta atttgttaga aaaatctaga tacgcatcta
    11221 aaatcgtttg gaagaaactt aatgttcctt caacgatatt accattttca tcaattagat
    11281 tcgcaacgtt tccgatatat gcttccggtt gctgcaaggt tggcacattc aaaaagacaa
    11341 gtgattgtcg tagatggtgg tttgcgccaa agccgccgat cccacctggt gatgcagtaa
    11401 cgactaaacc tggtttatga tcccaaacac tgtgtccata aggtcttgag cctacatcta
    11461 aagcattttt caatactgct ggcacagaac ggttgtattc aggagtaacg aaaacgacac
    11521 cgtctagtcc cctcacttcc tcacgaaaac gagtccattc agcaggagcc tgttctggag
    11581 tttcgagatc ctcattgtaa aatggcagat catcgatttt tacaaataca ctttcgtacc
    11641 cttcaggaag caatttcgca aaagcttctg ctactttttt gttgtaagaa tcttttctta
    11701 aactaccaat aaaaaatcca attttttttg tcatacgatt tttcctcctt caaactatct
    11761 ataacaatct tacaaaaaat aagtaactgg agcaaataaa aacacttact gacaaaagct
    11821 acatagaaga tagattactt atttttaaca aaaaagaagt ctaaaatctt ctatttccta
    11881 aaaaaaatcg aatttaatca agctgtaaag aaaaaaagct ttacagggta aaaaaaaact
    11941 gttacactac atcttgtgaa taaaacaatg gaggtggaaa taaataatgg cagttaaaat
    12001 ccgtttaaaa cgtatgggtt ctaaaaagag tccgttttac cgtatcgtcg tagctgattc
    12061 acgttctcct cgtgatggac gtttcatcga aactgtaggt acttacaatc ctttgaaaga
    12121 ccctgcagaa gtagttttaa aagaagactt agttcttgac tggttatcaa aaggtgcaca
    12181 accttctgac actgtacgta acatcctttc aaaagaaggc gttatgcaaa aacaccacga
    12241 agctaaattc tcaaagaaat aaggtgaccg gatatgaaag atttaagcga cttagtctta
    12301 acaatcgttc gtccgctagt cacttatcct gatcaagtca agttagacgt cgtagagtcg
    12361 aaagactttt acgagtacaa tctaacagtg gctcctgaag atattggccg tatcattgga
    12421 aaacaagggc gtgttgctaa agcaatccgc acaatcgttt acggtgttcg gatcaatgca
    12481 ccgaaaaaag tgcgtttgaa tatcatcgat gataaggaaa agtaaataaa cgtgctgtac
    12541 tcgaattttc gagtgcagct tttttgtttt tttattaatt gaagaaatga ttatttggca
    12601 aaacaagcaa gaaagcgtat ccttatagga ggagggagtg aacagccaat gagaaagaaa
    12661 atgttattta ttccatttgc tctattgtta agcgcttgta gttcggttac tcaagaatca
    12721 gacaatacac atctatcaag cagtatatct gaaactgtta ccagtccaga aaaaagtagt
    12781 accaaaacgg ctaccaccac tgaacaaaca atggctacct caaataatga gaaaaagact
    12841 gctttagacc agttgaaaga acaacagcca aatgttccca tgcctctaga tgtaccagtc
    12901 tcttcaggct atctcaatat cgcagcgaca catacaaagc aaggttattc tattttatat
    12961 tacagaacag atcgtcccct cgggttgaat gctgacgaat tgaatcaaga aacgcctatt
    13021 gcaacctatc tatatcaata tgggtttgct tctagtcaag aaacaatcca agttttgcag
    13081 ccctttgaga tcgacacaaa tggtcagcaa gtggacttag gttctcgaat cacagggtat
    13141 cagcaaggtg cagcaggttc aagttttctt gaatggcaag aaggaaactg gcgtattcga
    13201 atccgtggga ataatataga aggccaagat cccttactat tagctaaaga aatcgtagct
    13261 tatttggaag aaaacagtct accagcacct gaacaatttg gtaaaattac tgttgatatg
    13321 ggtgatacga cgaatcgagc cgtagaagta agctggcaag aaccgaaaaa tgcctatacg
    13381 atcactcatc aagatcctat gtctgcgttg aagatggcag tttctatgaa gaggttgtga
    13441 aatcagcgcg ctgatctgag cccatcattt aactccaagt cacaggaagt aatcaaaaat
    13501 agcacgccgc atttttgcat aattttggct taatacctta aaagaaaact agaattgcct
    13561 cttcacacat gctaaaataa aacagacaaa aaagaaatgg ggatataagt tgactgaata
    13621 cttgaatgta ggaaaaatcg taaacacaca aggtatcaaa ggcgaagtcc gtgttatctc
    13681 aacaacggat ttcccagaag aacgttacaa aaaaggaacg gtgttaacac tattccaaga
    13741 cggaaaagca cctgtggaat taacagtcaa aagtcatcgc aaacataaaa attttgactt
    13801 attgagtttt gaaggacatc cttctatcaa tgatgtggaa aaatatcggg atggcatatt
    13861 aaaagtgtca aaagacaact ctgtagatct agctgaaaat gaattctatt accatgagat
    13921 catcggtctg catgtattag aagatggaac tgagttaggt aaagtaaaag aaatcttgtc
    13981 accaggtgcc aatgatgttt gggtcgttca acgtccgaaa aaagctgata tattgcttcc
    14041 atatatcgct tctgtcgtca aagaagtcga tttagaagct ggagtggtcc gtgtagaaat
    14101 tccagaagga ctgattgacg atgaagattg atgtattgac tttatttcca agaatgtttg
    14161 aaggtcctat gggggaatcc atcatcggta aggctgtaga taaaggatta ttagatatca
    14221 atatctctaa cttccgcgat tattcagata acaaacatca gacagtagat gattatccat
    14281 acggcggtgg agcaggtatg ctgctcaaag tccagcccat ctatgataac atccaagcca
    14341 ttgaaaaagc tgcaccggat gtcaaaaaaa gagtaattct tcttgaccca gctggtaaac
    14401 gattcgatca gaaaatggca gaagaatttt cacaagaaga acacttagta ttcatttgtg
    14461 gtcattatga gggatatgat gaacgtatcc gcacattagt gacagatgaa gtttccttag
    14521 gagattatgt ccttactggg ggagaattag gtgcgatggt catgatcgat gcaacagttc
    14581 ggcttttgcc agatgtgtta gggaatcaga cttcagctca aacggattct tattcaaccg
    14641 gtttattgga acatccccaa tatactcgcc cggcagaata taaagggatg aaagtaccag
    14701 aagtattgac aaatggaaat cataaattga ttgaagaatg gcaattaaaa gaatcactaa
    14761 gaaggaccta tcaacgtcgg cctgatcttt tggaaacact tgaatggaca cctcaaatga
    14821 cgaaattttt agaagaaatc aagaaagaag aacaagaaaa aacagcagat gaagccaagt
    14881 gactttttct aaaatattct ttacagtagg ttgataatat gttagtattt tatggtgagt
    14941 gcaaaagcac ttaactatta cgatattccg ctatgagaaa agctcataag aatatttgga
    15001 ataaggagaa gaaaaccatg aatccattaa tcgaagaatt aacaaaagaa caattacgtt
    15061 ctgacatccc agcgttccgc cctggtgaca ctgtgcgtgt tcacgcaaaa gttgttgaag
    15121 gtactcgtga acgtatccag ttatttgaag gtgttgttat caaacgccgc ggtgctggaa
    15181 tcagcgaaac ttatacagta cgtaaagttt ctaacggagt gggcgttgaa cgtacattcc
    15241 cattgcatac accacgtgtt gcaaaaatcg aagtagttcg ctacggtaaa gtacgtcgtg
    15301 caaaattgta ctacctacgt gcattacacg gaaaagctgc tcgtatcaaa gaaatccgtc
    15361 gttaatcatt gttatcagac tgagaaagaa cccttggtat tcaagggttc ttttttctta
    15421 gcatgtaatt tgaacaccct gatccagttt ttttctttaa ttattggtag gggagaataa
    15481 tggattagaa taaatctacc tataagtagt agtaaaaaaa agagcactat gataatatct
    15541 ttcttaagag taaccgcttt aataaaaaat ccagaatatt ctaacataaa tggagtattc
    15601 ttagaatcaa aaggagaaag aagatggttt atacttatac ggagcaagaa aaaatgaaag
    15661 tttgagacac attcatgaat gatggaaaag taaaacaagt accaagttca acaaaaaaga
    15721 aaaacatcct attgaaagaa gtcttgaaac gctttgacca tggagtgact tatacagaaa
    15781 cagaggttaa ttcgatactg ctgaacgtat tttctagtgg cgattatgtc gaacaaagac
    15841 gttatctaat cacatttggc tttttcaaaa gaagttcaga cggtcgggca tatcagatga
    15901 tgggaattga aaattagaag ttttaattta tactcattga aaaaagttca agtatgcgat
    15961 attggttcta caccgcatac ttgaactttt tttgtcatct gtatctataa tacaaagcca
    16021 agaacgttat tcaaaatatg gatagctatg ataaaagcta atgagcgatt cgcagttaaa
    16081 taagcaatcg tcagtaaaat cgttccagga gcgtacatga aaaaatcaat tggatgcgtt
    16141 acatgaataa agataattct cttttatttg tacgaataaa taccaaaacg tacctcctaa
    16201 aattagattc aaagaagata gcaaagagga aaaagatagt tctgcagcag ctatagcggc
    16261 atgtggattg ctagaacttt ccaaacaact gcctgtttca gatcctcgtc atttcgatta
    16321 cgaagagata gcaatcaaaa tcttggaaga attacagaaa aactatacaa cgaaacgagt
    16381 taacggatca aacggtctgc ttctccatgc cgtttatgat aaaaacagct taaaaggtgt
    16441 agatgaatgt gtgatctggg gcgattactt ttatgtagaa ggaatcactc gtctagctaa
    16501 aacatggtac tgttactggt gatgtataag agaagaggag gattcatata atgaagcgat
    16561 ggaaaaaaat gatgattgga tcaatagttt taggaatgtc actcgtattt ggtgcttgtg
    16621 gtaatcaaga agacagtagt gaagaatcag caaatgaaat taccgtttcg acatggaatt
    16681 atgaaacaac acctgaattc aaagcgctct ttgaagcatt cgaaaagaaa acgggaatca
    16741 aagtgaaagt agtagatatt gcttcagatg attatgatac gaaactcacc acgatgcttt
    16801 cttctggaga cacgacagat gtattgacga tgaaaaatct tttgtcgtat tcaaattatg
    16861 ctttacgaga tcagttagtc gatcaaacag aacgaattag agaattagac actgatgctg
    16921 ctgaagaaac atatgaaatg tatgatatcg atggaaaaac atatgcattg ccatatcgaa
    16981 cggacttttg ggtactatat tacaataaga aaatgtttga tgatgcagga atcgaatatc
    17041 caaaaaatct aacttgggac gaatatgaag atttagcaaa aaaacttagt aaaaatgacg
    17101 gacaagttta cggagcatat caacatatct ggcgttcgac aatccaagct attgcagctg
    17161 ctcaaaacga ggcgaatctt gttgaaccag attatcaata tatgactgat tattatgatc
    17221 gagcattgag gatgcaaaaa gaaggcgcgc agatggattt tggcacggct aaatcaacga
    17281 aagtaaccta tcagtctcaa tttgaagaac aaaaagcagc aatgatgtac atgggaactt
    17341 ggtatatggc tggcatactc gcaaacaaag aagcaagtaa gacagaagta gaatggggaa
    17401 ttacagctat tcctcagaag aaaaaaggag aatcggtcac gacgtttggc tcaccaacag
    17461 catttgctgt caataaaaac tcaaagaaac aaaaagcggc gcaagaattt attgaatttg
    17521 cagccagtga agaaggagca aaagtattag caggagtagg agtagttcct tcatatcgta
    17581 cggatgagat tgatcagctt tattttaatc tcgctggaat gccaacggat gaaatttcta
    17641 aaaaagcttt ttctcctgat gaaatcaaac ttgaatttcc gattgatacg tatgggcctg
    17701 caatcgataa gattttacaa gaagaatatg acttagtttt ggttggagat gaaacgccgg
    17761 aaaaaggaac ggtaaatatg gagaaacgtg tagctgcgga aaaagaataa tcatgaaagg
    17821 agagaagcca agagataaaa cactcttggt ttcgtcatcg aaatggagaa tagtataaat
    17881 aagaaagcta caaatcattt gcgtagaaaa cgaacgtgga tcgcttattc gtttatcgcc
    17941 ccgaatttca ttggtttttt cgtttttaca ttaataccaa tcctgttttc gcttgtacta
    18001 gcatttatga aatgggattc tttttcaacg ccagaatttg ctggactaga taactttcgt
    18061 aaaatggcca acgatgagac attttggatt tctttgaaaa atactttttt atataccgct
    18121 gctgtagtgc ctggcacact tgtttgttcg ttagggctag ctttactact aaatcagaaa
    18181 attagaggga tgaagctttt tcgtacgata tttttctttc cgtatgttac ttccttagta
    18241 gcaattgctg ttgtttggaa tatgttgttt catccaacag tgggacctat taatatcttt
    18301 ttaagacgat ttattgaaca tcctccaggt tggacttcaa gttcaaattg ggcgctttgg
    18361 gcaattgtaa ttgtcagtat ttggcgaggg atgggttatt acatgattct ataccttgca
    18421 ggactacaaa gtatccaaaa agaactatat gaagcgtctt cgctagatgg agcaaaccgt
    18481 tggaaacaat ttttgaatgt gacattacct tcgttgagac caacaacctt ctttgtaacg
    18541 atcatgcttg tcatcaattg tttcaaggtg tttgaccttg tgcaagtgat gacggatggc
    18601 ggccctggtc gagcgacaaa tgtgctggtt taccaaatct acaatgaagc attcaaaaaa
    18661 tttgattttg gttatgcttc agctatttca cttgttttgt tcgtaattgt tttgggcgtt
    18721 acggttgttc aattttcttg gaataagcgt cgtacttagg aggtgtgata tggaagagag
    18781 tgttcgaaat gaaagaaaaa aagcaaaaat tcgttcaaaa aacatgccct ttattcaatg
    18841 gttgggtatg attctgctga ttgtattagc tttattgacg atcattcctt ttttatggat
    18901 ggtttctgct tcattcaaag caaataatga agtgttaacg attccgattc aatggatccc
    18961 taaaacttgg catttagaga attatctggt tatttggtct cgtattcctt tagtgacatt
    19021 ttttaagaat acagcttttt taagcgttgt gatcacgttg attcagttgt ttacttctag
    19081 ttttgctgtt tacgggtttt ctgaactaaa ttttaaaggg cgcgatattt tatttttagc
    19141 atatatcggc acgattgctg tcccttggca gtcttatatg atcccgcagt tcattatgat
    19201 gagaaaagtg ggtttaacag atacgttgtg gtcgttggtg ctattacaag cgttcaatgc
    19261 ttttggtgtg ttcttgttga agcaatatta cagtagtatc ccagattctt tatgtgagag
    19321 tgcgcggatc gatggcttat cagagtggga aatctatcgc aagatcattt tacctcttac
    19381 aaaaccggcg attgccagtt tgacgatcat cacattcgtt aatacatgga acgattatat
    19441 ggggccattc atttatttgt cttctacgga aaataaaaca atccagttag ggttgaaaat
    19501 gtttattggg ctatttgatg cagagtacgc attaatcatg gctgcctcag ttgtttctgt
    19561 tcttcctgtt gcttttatat ttttagcgat gcaaaaatat tttgtagaag ggatcgcttc
    19621 atcaggaatg aaggaatagg aggtgattat gaaagagcag cgttttgaca aaaatgttta
    19681 tatggaattc ttttattggt tgtatcaatc attggcaagt agcctttgtt tttggtgtgt
    19741 caatatcttg tatatagcag ctgcgttcct tttgtcgata gatggaagga atgtcttttc
    19801 ctttattttt tctttacttt ttattggtcc aggttgcatc gtgatgatag gtatgacaga
    19861 agaattagca aaagaaaaaa gtttttcacc agtacgtttg ttcataaaga cgtttcgcca
    19921 attttggcta aagggatttt tttattggct atttgcttgg atagttagtg tgataatgat
    19981 ttttgattgt ttctttttta tacgtttttc ttatggaaaa tggttgattc cgttgtttgt
    20041 tttacttgct tgttttactt gcttgtttat cagtttcctt tagcatcaac tgctggtatt
    20101 ttcaggtgag aaatccagcg tcgaaaccaa atcaagttct acggatagcg ttctactata
    20161 cgttgaaaaa atggtatgtg agtttactag attttttact attgacaagt ttgtttctct
    20221 tctttttcgt aaagccccaa tggtgtattc tactaggtcc ttctatcgta tttggtttga
    20281 tttacttcaa caatcgtaaa ttgatgagaa ctatggatct gtagacttta taaattgaaa
    20341 ctttcaatta agaatgacag ttttttatgt aaaaactgtc attctttttc attttttgaa
    20401 acatatggct aaacaaatga aataaaacat gctatacttt tgaatgaaac aggtttagaa
    20461 actgtatcac ttatttgtga ttatttctaa ctaataatta aaaataaagc ataaaaagga
    20521 cgatgttcaa tggctgaaaa aatgaaacaa ggttcgaaat ctgtagtaga aagtttgatt
    20581 aatcatcatg ttgattatgt attcggtata ccaggtgcaa aaatcgatgg tgtatttaat
    20641 gaattagagg atcaaggtcc tgaattaatc gtgactcgtc acgaacaaaa tgctgcattt
    20701 atggcacaag caatcggacg tattacagga gagccaggag tagttatcgc aacaagtggt
    20761 cctggtgcta gtaacttagc tacaggtttg gtaacagcga cagcagaagg tgacccagtt
    20821 ttagcaattg ccggacaagt aaaacgaagc gatctattga agttgaccca tcaaagcatg
    20881 gataatgcag cactgttcca gccaatcaca aaatacagtg cagaaatcca agatccagaa
    20941 acaatttcag aagtgattgc caatgcttat cgtatggcta agagttcaaa aaaaggcgcg
    21001 agctttatca gtatcccgca agatgtagtg gatgcacctg tccaaggtaa tgtcatcaag
    21061 ccattgagcg atcctaaatt aggttctgct tctgcagatg atatccgtta cctagctgaa
    21121 cgcattagag aagcaaaact accggtacta ttagtaggta tgcgaggatc aagtgaaaaa
    21181 gaaacgttag ccatcaggca attagttgaa aagacagcat tgcctgttgt agagacattc
    21241 caagcagctg gagtgatctc acgtgaatta gaagcgcatt tctttggtcg tgtgggttta
    21301 tttagaaacc aaccaggaga tatgcttttg aaacgaagtg atctagttat tgcaattggc
    21361 tatgatccaa tcgaatatga agcaagaaac tggaatgcgg aaaaagatgc gcggatcatt
    21421 gtgatcgatg aagcgccagc tgaaatcgat ccttttatgc agcctgaaag agaattgatc
    21481 ggtgatatct cagccactct tgatttattg acaggttcat tagagcctca acaagtgtca
    21541 gaagatgcaa aagaatattt agcatctctt caagcaaaat taacggagcg ggacattgtt
    21601 caatcaaaag gagaagcagg tatcttgcat cctttagaag ttattaacac gcttcagtca
    21661 aaagtaacag atgatatgac tgttaccgta gatgtaggta gtcattatat ttggatggca
    21721 cgccacttta gaagttatga acctcgtcat ttgttattca gtaatggtat gcaaacgctt
    21781 ggtgttgcat tgccatgggc gatttctgct gcattggtgc gtccaaatac acagatcgta
    21841 tcagtttccg gcgatggggg atttttattc tctgcccagg atttagaaac ggcggtacgt
    21901 aaaaaattga atatcgttca tttgatttgg aatgatggac actacaatat ggtagagttc
    21961 caagaaaaaa tgaaatatca gcgggcgtca ggtgtagact ttggtccagt tgattttgtg
    22021 aaatatgcag aagcttttgg cgctaaagga atccgagcta caagtgtaga ggaattagaa
    22081 aaagctttag aagaaggatt tgcgacagaa ggacccgtaa ttattgatat tccgattgat
    22141 taccgtgata atgagaaatt gggagaaaca atcttaccag atcaattcta ttaaggaagt
    22201 gtcgacatga ctgaaaaaat cttataccag cacgggacat taggagcttt gatggccggc
    22261 ttgatggatg gaacagaaac gattgctcat atattagaaa aaggaactct gggattggga
    22321 acattgcatg ggcttgatgg tgaagtcatc tttttagatg gcgtagctta tcaaggaaga
    22381 tcagatggct cagttgccca attagatgga agtgaactta ccccctatgc agcaattaca
    22441 gattttacgc cagatactag tttttctgtc tcaaaaactg cagatggaga acaattgaaa
    22501 aaagacatat tagtaagaga agccggggaa aacctatttt tggcagtgaa gataactgga
    22561 ttattcaaaa atatgcacat ccgtatcatg ccgaagcaag aaaaacctta cagaagacta
    22621 gcaaaaattt cagagtctca accagaattc caacaaagca atgtccaagg aacattagtc
    22681 gggttcttta cgcctgaatt attccaagga gttgctgcag cgggatttca cctgcatttt
    22741 atcgatgata cacatacatt tggcggacat gtaatggatt ttgaagtggc tgaaggaaaa
    22801 gttgagatta gccgcatctc gtcccttgta cagcattttc cagttgatga tccgactttt
    22861 gttcacgaaa cgattgatta tgctgattta gcagcagaaa tccaacaagc agaatagaaa
    22921 aagacagcga gatgcgttca aggtgattaa cctgacgtat ctcgtttttt tgaggagaaa
    22981 acctagcgtt tacaaaaaaa ctgagctata atcttctcca tagatcaaaa aggagcggtt
    23041 tttatcatgt ggacagaaaa acagaaaaaa gatgcatacg aaattttatt attacagcaa
    23101 caaggattat tagaagcaga agataactgg ttagcaaatt tagcaaattc atctgcattg
    23161 ttgaatgaga ctttgcctga gactgtcttt gcaggatatt atttattcga tggtaatgaa
    23221 ttgattttag gaccgttcca agggcgagtt tcttgcacac gtatcaagat gggaaaagga
    23281 gtttgtggag aatcagctga aaaacaagaa acattgatcg ttgatgatgt caaaacccat
    23341 gcaaattaca tttcctgcga ttcagccgca caatcagaaa tcgtagtgcc aatggtaaaa
    23401 gaaggtaatt taatcggtgt tttagatatc gattgcggag taacgaaagg gtatgatgaa
    23461 atcgatcaag catttctaga aaaatatgta gatcttttat tagcaaattt taatataaat
    23521 gctttttcat ttattaataa ctaaaatcgt ctattgaaac aaaaaagaag agaaatcaag
    23581 aataaaaaga gccggaaaga cagtgggcat ttctgttttt cggttttttt atttttttat
    23641 aaaatcacaa gtgttattga ttttcataat catattttaa tgttattatc attttaaata
    23701 atgaaaaaac attaaggatg gatgaaatat gaataacgaa actatcattt taggaaaaaa
    23761 atatatttgc caacctattg gcttgaaaca aaaagtgatt ggagaagtaa tcagtaaatt
    23821 agaaaattgt gttgtgatct gcgtagacga atatcatgac gtcgatcgca atgaaatatt
    23881 agaaaaatgc ggaaaagtag tggcaaaata tactcacgtt tatgagttgg aaacaacaga
    23941 atatctatat aactatagca acaaatatga acatacttta gtttcataaa gatttaaata
    24001 aagtgccttc aaaagcgaga agaccaagct tttgaaggca ctttatttag ttggcagtga
    24061 ttgttcatga tttttgattt attcaaaagg ttcgcctaga gaatctgtct ctgtaacgag
    24121 gcctggtgga gaaactcgat aaaaacctat actgccagaa ctgtcctatt ttcggataga
    24181 caaactagta gttttaaaaa gatcatccga tccaatcatt tcaaaaaatg caaaactgat
    24241 atcaggatca tttagctcag gcttgttatc gtaaagcatt tgtaaaggtt gcatttagtt
    24301 aggagtaaac gcgaaactat catcattatt tgctgaagaa gatgaagaag ctaattcaga
    24361 gtttgtttga aataaaggag acgtgctgac agtttcttta ccctgttttt tgcagaggta
    24421 gaaactttta tatcggaagt ttctaccgga gaatttttct catttgtaga aattgttgaa
    24481 tcgatactat tcgaaatttg cgtatctgaa ggtatgttct ttgatgcaga agtcccacaa
    24541 gcagtaagta acaataaatt tgataaactg ataaacagaa ttcttttcat tatgtacaca
    24601 ctccttatag tatcgtttag catacaccta tgctcagtat taggcaaaat caatgcaaaa
    24661 ctatcaagtc ttttatagta tctattagtc ttagtaatac atatatggag agggtgatat
    24721 cccagttcaa accagataca ttggttacca tctgtattaa cgattatgcc tctaaacgct
    24781 ttttttatga aaaaatttat aagtgaaaac gtaggtatga tattatctag aggtaatttt
    24841 tttgggtatt taaatagttg ctaggaggat agcttttgga tttttacagt aattttatct
    24901 taataatagc aatattatta cttctaaata tttggttttt tgataagagt agaaatgcag
    24961 ggataggttt tagaaccaaa cgaagtacgt cttctgaaaa aaagtgggta tattcacaga
    25021 ctatttttta cggaggggtt atttcaatca gtttactttc ttcaacactt tattctttta
    25081 atgttataga cgtttccatg tcaaatttta tatctattat tggaatcctt atttctgcaa
    25141 ttattacaca actacttctt gtgtttgaag agaagtcaaa aaataattaa agcaattatg
    25201 ccttttagta caggaattat tacttcttac aaatgtatag cgaatgtgaa gcctgctgtt
    25261 ttaattgttc tagaaaaact tttgagtgaa caattatttt atgttaggat gaattaattt
    25321 ctctagagtt tata
//
LOCUS       DAYYCW010000031        24877 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000031 DAYYCW010000000
VERSION     DAYYCW010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.031"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..645)
                     /locus_tag="ACHAYA_001186"
     CDS             complement(34..645)
                     /locus_tag="ACHAYA_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8604995.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(768..893)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(975..1691)
                     /locus_tag="ACHAYA_001187"
     CDS             complement(975..1691)
                     /locus_tag="ACHAYA_001187"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8604996.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1697..2266)
                     /gene="scpB"
                     /locus_tag="ACHAYA_001188"
     CDS             complement(1697..2266)
                     /gene="scpB"
                     /locus_tag="ACHAYA_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM8604997.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2250..3020)
                     /locus_tag="ACHAYA_001189"
     CDS             complement(2250..3020)
                     /locus_tag="ACHAYA_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM8604998.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3017..3388)
                     /locus_tag="ACHAYA_001190"
     CDS             complement(3017..3388)
                     /locus_tag="ACHAYA_001190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8604999.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3407..4294)
                     /gene="xerD"
                     /locus_tag="ACHAYA_001191"
     CDS             complement(3407..4294)
                     /gene="xerD"
                     /locus_tag="ACHAYA_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM8605000.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4390..4863)
                     /locus_tag="ACHAYA_001192"
     CDS             complement(4390..4863)
                     /locus_tag="ACHAYA_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8605001.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(4968..5831)
                     /locus_tag="ACHAYA_001193"
     CDS             complement(4968..5831)
                     /locus_tag="ACHAYA_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM8605002.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6027..8126)
                     /locus_tag="ACHAYA_001194"
     CDS             complement(6027..8126)
                     /locus_tag="ACHAYA_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM8605003.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(8346..9455)
                     /gene="rpoD"
                     /locus_tag="ACHAYA_001195"
     CDS             complement(8346..9455)
                     /gene="rpoD"
                     /locus_tag="ACHAYA_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM8605004.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(9479..11320)
                     /gene="dnaG"
                     /locus_tag="ACHAYA_001196"
     CDS             complement(9479..11320)
                     /gene="dnaG"
                     /locus_tag="ACHAYA_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM8605005.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(11502..13817)
                     /locus_tag="ACHAYA_001197"
     CDS             complement(11502..13817)
                     /locus_tag="ACHAYA_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8605006.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(14002..14862)
                     /locus_tag="ACHAYA_001198"
     CDS             complement(14002..14862)
                     /locus_tag="ACHAYA_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM8605007.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(14921..15553)
                     /locus_tag="ACHAYA_001199"
                     /pseudo
     CDS             complement(14921..15553)
                     /locus_tag="ACHAYA_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            15720..16481
                     /locus_tag="ACHAYA_001200"
     CDS             15720..16481
                     /locus_tag="ACHAYA_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8605008.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            16507..19098
                     /gene="mprF"
                     /locus_tag="ACHAYA_001201"
     CDS             16507..19098
                     /gene="mprF"
                     /locus_tag="ACHAYA_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8605009.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19286..20473)
                     /gene="tuf"
                     /locus_tag="ACHAYA_001202"
     CDS             complement(19286..20473)
                     /gene="tuf"
                     /locus_tag="ACHAYA_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8605010.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            20841..21197
                     /locus_tag="ACHAYA_001203"
     CDS             20841..21197
                     /locus_tag="ACHAYA_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8605011.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(21438..22442)
                     /locus_tag="ACHAYA_001204"
     CDS             complement(21438..22442)
                     /locus_tag="ACHAYA_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM8605012.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            22608..23459
                     /locus_tag="ACHAYA_001205"
     CDS             22608..23459
                     /locus_tag="ACHAYA_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8605013.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            23476..23721
                     /locus_tag="ACHAYA_001206"
     CDS             23476..23721
                     /locus_tag="ACHAYA_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605014.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            23908..24612
                     /locus_tag="ACHAYA_001207"
     CDS             23908..24612
                     /locus_tag="ACHAYA_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605015.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         6992 a         5162 c         4351 g         8372 t
ORIGIN      
        1 aaagaccaag aaatttcttg atctttttga tatttatttg actgctgtat gacgttgttc
       61 gaactgatgt tgttttcgac ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac
      121 agcactcacg atgaatcctt tgatcaagtt gaaaggaacg atcccaatca gtacatagtt
      181 tgctaaagac atgcccagca tctgtgttgc gttcagccct aaaaacatta agtaaagagg
      241 cgtaataacg aaataatttg cgatactcat aaaaatagtt agagcgatgg ttcctgtaat
      301 gacacctaaa aatttgtttc ttcctttatg ctgtttcttt acactaaaga aataataaat
      361 cgggaatgta aagatcgttg ttgataagaa actagcagtg tctcctacca aattatccgg
      421 agaaaaacct gtagtcagca gatgtaatac tgaacgcaag aacgctgttg caactccggc
      481 cgctggaccg aataagaaca tgctgatcaa taccgagata tcactaaaat cgatcttcaa
      541 aaaactaaaa gccggcatga tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc
      601 aaacatcgct actgcaacca ttttctgtac acgggtgttc ttcatttgaa atcctcctgt
      661 taggactcct cttcagcgaa ctttttgacg tttctctcca aatgaaaaaa ctctattttc
      721 cagggaaaat agagttagag ttatttgatc aagcgtcgtc aaataagctc tatcttcttt
      781 atccagacta tactgtcggt atcggaattt caccgattca gctatttttt tgcaaaatag
      841 gtcgtggact ataaccaccg gtcgggaatt tcaccctgcc cctgaagacg aaccttatat
      901 aatttttgtt tacatattca ttatacatat ctattcaaaa aacgcaacat ctaacactta
      961 taaaaagtaa gtgttcattt tgagtggttc agcaaactgc tgacttcttt tttgttcagt
     1021 tcacggtaat ccccaggacg taggccttgt aaagtcagat ctccgtattt ttcacgtttt
     1081 agtttttgca caggaagacc aactgcttga agcatatttt tcacttgatg attacgtcct
     1141 tcgtggatcg tcaattccac gatactgtgg tttgtcttag gatccactga taaaatttgg
     1201 aatttagctg gagatgtttt tcgtccttcg atctttattc cttttgtcaa aggtgcaagc
     1261 atcgattttg tagcgatccc ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt
     1321 ggatgtgtca ggcgctgagc aaaatctcca tcatttgtca gtagcaagat ccccgaagta
     1381 tcgtaatcca gtcgacctac aggatagata cgttctttta ccatcggcaa aagatccgta
     1441 acaacttttc ttcctttgtc atctgcaaca gcagaaataa ctcctttagg tttgtacaat
     1501 aaatagtaac cgtattcttc ttgataaatt gggacttcat ccacttccac agtgtcatgt
     1561 ttcccaactt ttatccctaa ttcccttaca acttgtccgt ttacttttac acgtcctgca
     1621 aggatgaatt cttctgcttt tcgacgagaa gtgatgccag cgtgggcaat tactttctgc
     1681 aatctctcca ttacaattat tctcctttat tcattccatc aaagaataga tctggtgaca
     1741 cgtcttcttc aagtgaaatt tccatttctt gtgtatcagg cagttcttct aatgatttca
     1801 aaccaaaata atctaagaag tagccagttg ttccatataa tatcggccga cctggtcctt
     1861 ctactctacc tttttcttca atcagtttat gggcagctag acgctgtacc gctcctgaaa
     1921 cctgaactcc tcgaatctgc tcgatttcag ctcttgtgat tggctgttta taagcgatga
     1981 ttgctaaggt ttcgacggcg gcttgagata atgtgtttga tgtcccttgc gcatatcgct
     2041 taagtaaggg agccagctct tttttagtcg ttaatacata atggctttcc gtttcaaaaa
     2101 tatgcaaagc tgagtgcgag ttttgagtgt aagattcttt taattctttc actaaacttg
     2161 tgacttctgc ttcgctgaga tttaacagtc cgcttaattc ttcattactg atcccttcct
     2221 cgccaacaat aaaaagaagg gcttcaagtt cacttaattt cgtcatgttc ggtatctcct
     2281 actgctggat aaagcataat tgattcatag ttttcttttt gttgcgcaat gatctgccct
     2341 tttttcatca attctagcaa agccataaaa gtagtgacaa cttcactttt agaaggtgac
     2401 tcaaaaaaag aatcgaaagt acagccatgc gcatttcttt ttatacgctc ttcgataaat
     2461 accattcgtt cttcgatcgt tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa
     2521 aatttcttac gttctaagat tttctgcatg gcacggatca aatcgatttt tttgacttgt
     2581 cctcgcttca acggcttttc agcatccgct aacccatcga tgttcatagg ttcttttgtg
     2641 taataaagac ttcgttcctc tgctttttct gtcagtcttt cagctgcata tttatattta
     2701 cggtattcta aaagctgagt gaccaattct tctcgaggat cttcttccca taattcttca
     2761 tcgtctgtga cgaattcttg gacaggaagc aatgtcttgc ttttgattgc catcaatgta
     2821 gcagccatca ctaagtattc tcctgcaaca gctagctcga gtgttttcat tgtatggata
     2881 taattcatat attgctccgt gatcgtagcg atcggtatat catatatatc gatttccatt
     2941 tgctgaatca gatgaagcag cagatcgaga ggaccctcga atacatctaa tttaatattg
     3001 atctccgtca actcattcac tctttcttct ttttctttgc tagccatttt ccaatgtaag
     3061 gctctgtgat ttcagttgca cggatggaga catccgggta cttttcctgt aattcatcta
     3121 acatttgaaa gcccatattc tcaccgcgat aagacggatt caaagaaatg tcatggatga
     3181 tcagctgatc gttcgttgcc cagtaaatac caataatccc ttgaatgttg gttgaacctt
     3241 ctggcgtaaa gaaaaaaagt tcataatcgt cttcagattc gtacgtatca atctccttca
     3301 acaaagaagt tttacctgtc agtttcttat ggaaactcaa tagtcccata gcaattttac
     3361 ggttttcttt ccggtaatgt gttaacatct tctcaccttc ctatgactaa gcccttggaa
     3421 aatgctgctt ataaacatcc gtcattcttt tcttcgtgat atgtgtataa atctgagtcg
     3481 tagagatatc cgcgtgacct aacaattcct gtactgtccg caaatcagct ccattttcta
     3541 gcagatgtgt ggcaaagcta tgccgcagcg tatgtggtgt tacttctttg taaatcccag
     3601 cctctcgtac taactgcttc agatttttcc agattccttg ccgagaaaga cctttcccat
     3661 gattattgac aaatacatgc atttcagatg cgtcctttac aagtaatgga cgtgcttcgt
     3721 ctaagtagcg ttccagccat tggatcgcat aatcacctaa aggaatgatc cgctctttat
     3781 ctccttttcc aactgtctgt accagtccaa gagaaagatg aagatctccc aatttcaatc
     3841 caatcaattc actgacgcgc atcccagtag catacatgac ttccaagatt gcacgatctc
     3901 ttatccctaa cgtttttgtc gtatcaggtg cttcaatcag cttttctact tctgtcagag
     3961 tcaatgtact tggtaatttc tgtgctttct tcggcgtatc gatatgttgc atcggatcat
     4021 gatcagtgat acgctcttgc cgtaaaaact gatggaatct tctgagactt gagatcatcc
     4081 gagtaacagt tgctgtcgct tttttttctt catgcaactt ctccagaaaa cggatgacta
     4141 aaaagcgatc gacatcctgc catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact
     4201 gttccaaatc acgttcatag ctttgtcgtg tattggcgga caatccccgt tcaatattca
     4261 agtagtggag ataatcgttg atttgctctt tcataataat ccccctcatg cctctctatc
     4321 atatcagata ccgcttcatt ctgatagtct ttttccataa tctgagcaaa gaaaaagccg
     4381 tctagacact cagacggact ggtttctttt ttctttttca tagcattcct gacagattcc
     4441 atggaacgtt agccgatgat cttttactaa aaaatgataa cgttcttcta ctacttgctc
     4501 cacttcaagt aaaagatcct cttctacttc ctcgatcgtg ccgcagttca agcatagtaa
     4561 atgatgatgg aaatgtttgg cgccttcttt tctcaaatca taacgggcta atccatcatt
     4621 aaaacttact ttatcgacaa ttttcaattc tgtcaagatt tcaagggttc gataaacagt
     4681 agcaagtcca atttccggac tctttttttt cactaaaaag aagatttctt ctgcagataa
     4741 atgctctttc tcattttcca aaagcacaag cagagtagct tctctttgcg gtgtcagctt
     4801 aaaccccgct tcatgtagct gctgttttgt ttttttcatc gccgaaatag cattcatgac
     4861 catttactca accccaacaa tttataataa ttacaaataa aaaaagatat aacactatgt
     4921 taattaaaat tattataagc tagttaagaa aaacatgcaa gggattctca attagatttt
     4981 ttgactagtt ttgtcacacc gttttcttgt gtaatcaatc cttgtttcaa aagattacct
     5041 aatgcgcgct taaatgctcc tttgctgata ccaaaagctt tcatgatttc ttctggatcc
     5101 gatttatctg taaagttgat ttgctgatct gcagaacgtt ctaaaatggt taaaatcatt
     5161 tgtgcatcat ctgatatagc ttcatatgct cttggcttca atgaaagatt cagtacaccg
     5221 tctggtcgga caccaatcac tcgacctttt atctgctcgc ctagacgagg ttcattatat
     5281 cgttcagaag gatggataaa cccaagatag aaatcttccg ttaaaacgta cgtacctgtt
     5341 aatttcaagc ggtatgctgt tcctatcaca tctttgtttt tcatttcttc tgttccatat
     5401 ttgcttaaag atttgaatat tttttcatct gctaaactgc cccaaatacg gtcttttgca
     5461 tcaactctaa gggaaatcat caagcgatcc ccttttttag gccatagctc tgtcattgtc
     5521 ggcaattcat caagagatac gacaaagtct ttatctggaa gaccgatatc gacaaacgca
     5581 cctaaatctt ttcgtgtagc agtaacgatt ccaaaagcgt aatgaccgat acggctttta
     5641 ggaatttgtg tagtgatccg attttcatgt ttttgatttt ggtaaccgaa tccttcaact
     5701 gcttcaccga ttttgtgttc accttctgat ttgcttaagc caaaggtttg tccgtttttt
     5761 tggacaaagt agtttttttc attttcatca ataataatac ctgtaaatat agttgctaat
     5821 aaatcgttca tttttttctc cctctatagt gaaaggtttt cttaatacat ttctttgcta
     5881 tcataacata atgttatcta cttgaatagc tgagccttgg tttggaaagg tgaaaaaagg
     5941 agtatgacaa cagttgtgtc atactcccct ttttcgaata aacggtgtta aacggcaggc
     6001 tcacagcctc tttcatttgc ctaaaattaa ttgctcccat ttcctccgaa ccatccagtt
     6061 ttacagttga tggttacgat gtaaaatggc aaagcacttt ctccaatgcc tttcatcaat
     6121 gcagattttg ttgaagtggc atatagatta taatagcctt ccccattttc gattctggct
     6181 ttttctaatc tgtaacctcc ttcaacgata tatccacgtt ggatacatgt agcaatcact
     6241 ttgctttgga cacctggtgc ccacgtccat gctggattag atgcatcggc aatatcactt
     6301 gctctggcag atttgattgg ctgattcgct gagtttgaag aggaaggggt cgaggatgcc
     6361 gcttcttgct gtgctgcttg tgcgaccgca gcttgttgag cggcttgagc tgcagctgct
     6421 gcttgagcgg cttgttttgc ttgttcttca gctgcttttt tcgcttcttc tgcttttttc
     6481 ttttcctctt cttctttttt ctctttatca gtgagcatct tatctacttg tttcaattgt
     6541 tcattcaact gagcagatag atctgtattt ttcacttttt ctactgcttc ttttgccgtc
     6601 ttgtattggt cgcgtgttgc tgaatccact acttttccgt ctttaaagac tatagctgtt
     6661 ttttctttag ctgtttgcaa ttcagcatat tgatttttag cttctgtttg tgcttgttga
     6721 atcaattttc caaaatctgt gttatccgtt tcgattgctt ggatctcttt atctgctttc
     6781 aacttgatgt catctgctag atgatcatcg acgatggcag gtgctatgaa ccactcattc
     6841 actttttgga tatctcggat cttagattgg atatcttggt aagttgtttc taactctgaa
     6901 tatccttttt cattcttaag actggataaa tcatttttta gttttgataa atcttgatga
     6961 atcatatctg cacgaataaa ttgatggtga tcatctgtat aaaaggcagc aagtttctca
     7021 tgaagttctt ccatttgttg ctgtcttttt gcttctgctg ctacttcggc ttctactttt
     7081 tgatgatggt cataataagc ccagcttcct ccaccgagta gaagtactgc tgctaaaacg
     7141 agataaggta ctttttttga ttttttcttt tgcgctttat ctggatctgt tttttctaag
     7201 tttgattctt gatgtgtttt ttcagtatct ggcacgattg ttattgattt cgcttctgct
     7261 tcaaacagct taggctggtt ctcttctttt tctatttttg cttcaagatc agtaccctct
     7321 ttgcttacag attgattttc agcatcggat tcactattta tttcatcggg ctctgttgac
     7381 tttttgtctg tgttttcgag tatttctttt ttctctgctt ccggcagttt attcgttttt
     7441 tccttttcta attgtttttc agtaaatact tcagtttttt ctgtgatatc cgtttttacg
     7501 tcttttattt ttactgtttc tttacctgtg gcgttttctc tatcagagtc tttttcagtt
     7561 tcttgttcct tttctggctc tactttgttt tcctgtgcaa ttttttcact gatcattttg
     7621 agcagctctt tcgcgtgctc ttcttttgta tcttcacgat gtgctttgat ataagcggat
     7681 agtatcgaat tttcttcctc attatcgacg atttctttca gttcagctac tcttcttttt
     7741 tgttttgatt caagcgtttc ttcttgattc tttcctgatt cttccgaagt ctcatgaaca
     7801 gttgctttct tctgttcatt tttctctata ttagttactt gatcgtgagc aggtggttct
     7861 tcgtcttctg gttctgcttt ttcgaataat tccattacag attctagagg taaatctttc
     7921 aattccgacc attcgattgg atcgtttaat tctgtatcag gaaagactgc atcttcagtt
     7981 gatttatcct gcatagctgc atctgtattt gtttcttcaa ttgattcttt tatttgctga
     8041 tcttctgcag ttatttgatg accgcagtct ggacagatag tattctgctc catttttttc
     8101 gatccgcaat ttggacattt tttcatcact tagcgatcgg ctcctttact aggtttacgc
     8161 taccatttaa tctatcatat tctatacgtc acgccaatag cttattttta ggatttactt
     8221 aaaaataatg aatgaatttc cttcctatag aaaaaaaagc ttgagactgc gtagtctcaa
     8281 gctttttttc ttttaaaata aaccaaacag ttttactgat ttctcttctg gtcaatagtc
     8341 cacgcttatt ctaagaaatc ttttaattgt tttgaacgag aaggatggcg caattttctt
     8401 aatgctttag cctcgatctg acgaatacgt tcccgagtaa caccaaatac tttccctact
     8461 tcttccaatg ttctcgtccg gccatcatct aaaccaaaac gtaagcgaag gacattctct
     8521 tcccgatcag tcaatgtatc tagtacatct tccaattgtt cttttagtaa ctcataagct
     8581 gcgtgttctg ctggacttgt tgcttcctgg tcttctatga aatcacctaa gtgagagtca
     8641 tcttcttcac cgattggcgt ttcaagggaa acaggttctt gcgcgatttt caagatttct
     8701 cggacttttt ctgttggaag atccatttcc gcaccgattt cttctggcgt aggttctctt
     8761 ccaaggtctt gcagcaattg acgttggata cgtatcaatt tattaattgt ttcaaccata
     8821 tgaactggaa tacggatcgt acgtgcttgg tcagcgattg ctcgagtgat ggcttgacga
     8881 atccaccatg tagcataggt tgaaaactta aatcctttgc ggtagtcaaa tttttcaact
     8941 gccttcatca gtcccatgtt tccttcttgg atcaagtcaa ggaactgcat gcctcgtcct
     9001 acataacgtt tagcgatact tactactaaa cgaaggtttg cttctgccag acgctgtttc
     9061 gcttcttggt cgccttcttc gattttcaat gctaacgcta cttcttcttc cgcagttaaa
     9121 agggagacac ggccaatttc tttcagatac atacgaaccg ggtcattgat ttttacaccg
     9181 gttggcgcag aaagatcttc tgtttttgct tgttcagctt ttttctcagc actcttcaag
     9241 ctgtgaattg atggatcacc attttcatcc accacactga tacctgcgtc ttcaatcttt
     9301 tggatcaatt tgtccatttc atctgcattc aaagtgaatg gtgtagcaag ctgattggat
     9361 aaatcatcat atacaacttg tcgtttgggt ttgttttctt tgatgaaagc tgctactgct
     9421 gcttcatacg tttttttatc tgttccattt gccataaaag aaggcccccc ttgagtagct
     9481 atttggcttg ttttaactgt ttcgttaaat tgatgatttc aatcgtcaat tcaagctcaa
     9541 gctgtttgtt tccagttcgt ctggcttctt gctgcatttg tttttttaat gttatttcat
     9601 cagctaagcc agatttgctg atcacatcta gcaagtcttg gatttctcgt ggcgaacttt
     9661 cttctgccac cggtaaatat tcgatttcca ccaccaaccg ccttaaattt tcatcttgca
     9721 aaaagtctaa aaactctgca aggttgaatt cataatgttg tgagacatat gcatcgaata
     9781 aaaaataaac ttctgcatat gcatcatgaa taaactgaaa ttctcgttct tttaaaatat
     9841 tacggacaga aacttcttta aatgcacgat ataatagaat ctgctctgcc ttttctactt
     9901 gcgtcaaagg tctctttttc cgtgcagttt cttgtctcgt aacctgagtg tgttgacttt
     9961 cctgaggttc ttgacgattt tgctgatttg tccttcgaag atcgcgcagt tgtttttgta
    10021 tcgtctctct gccgatctga aactctgttg acagttgaga tagatacata tcttgttcga
    10081 ttggtgaaac aactcggcta agttcaacta gtaattcttc gaggtattcg atcttgtctt
    10141 tttcattatc taaatttttc ccttgtttta aataacgtga tttaaaagta aagactgttt
    10201 cgcgcccatg agccactaat tcttggaaag atgcttctcc atacttacgg agatattcat
    10261 caggatcaag cttttctgga acgactacga cagaaagtgc taatcgactg ttttgccgaa
    10321 gcaattcaat ccctcgattg gttgcttcaa aacctgcgtt gtctccgtca tagcaaaaga
    10381 ccaattcttt cgtcagcttt tccatttgtt ggatctgttg agctgttaga cttgtcccca
    10441 tagaagctaa tccagttttg ataccagcct gccatgcagc aatcacatcc ataaaccctt
    10501 cgaacaacaa aatttctccg tttttccgga tatcgcttct tgctttatca agattaaata
    10561 aaacttgtct tttattgaac agttctgttt ctggactatt caagtatttg ggctgatctt
    10621 tttcctgccc tttttcaggt gcaagccagc gtcctgaaaa accgatcgtc tggccttgag
    10681 aattacgaag tggaaacatc actctttgat aaaaacgatc agacatacgg ccatcatcat
    10741 gcgtgacaaa caatccagag cgttctaaca attcatttga aattgattca ttcttgaata
    10801 cttgctgaag aaaagaacgc tcattaggtg caaaacctat gttgaattct tcaattaatt
    10861 ctaaagacaa cccacgactt tgcaaatatt ctaaagctgg ctgtccagct tttgtatgca
    10921 atagcatgtg atgatagatt tccgctgctt tttcatgagc agcaatcagt ttccccgtag
    10981 cagattcagg attttggaca gctatagtac gctgacgcca ttgatcttct aacggtatct
    11041 gttcgaagtc tgccactttt accactgctt ctggaaaact tagaccttct agttcttgaa
    11101 gaaaagtaaa cacgtttccg cctttaccac aaccaaagca atgaaaaatc tgcttgtctt
    11161 ctgcaacaga aaaagacggt gtacgttctt catgaaaagg acacagcccc aagtaatttt
    11221 taccagactt tttcaactgg acatattgtc caatcacctc aacgatattc gtttgactgc
    11281 gtactgtttc gataacctct tgaggaatcc gttgagccat tttttcaccc cctgctctat
    11341 tgatatcttt tgggtttaac agaagatatt tcaggagaat aaccggtaaa aaatcgcact
    11401 aactgaaatc agtgcgattt taaaggacac aattatacat gtttgattgt atcatatttc
    11461 attgcttttt caagtataac acatttctat ctcattaagc atcaatttgc tactcttttc
    11521 agtaactgat tttgaatatc agccttccag tacaatactc gaagaacaat atttacgagc
    11581 atgaatacaa agaagtatcc tacgctctct cccaacccta agtttacttc caaaattcca
    11641 tggaacagca ttgcagctac atatatccct acgatcgtcg ttactgctag gaagatgcgc
    11701 aatgggttga agggataaca agcttttaca actgccatgc aactgatccc aatcagcaag
    11761 taatacatca aagtagtagt ttcagcattt ccccatgcga atgtttttcc aagtagatac
    11821 accgcaatga tattcactac tacaagcaag gcatttggta aagcattaat aagtgctgta
    11881 ggcaaataac ggtaagtgat ttttctttta tcttgttcaa aagataaaaa gaaggaagga
    11941 tacccttcaa tcgctaaatc aatcagcgtg atttggatag ggataaacgg aaatccaatt
    12001 gctgtaacgg cacatataat cgataaaata aatgaataaa tcgttttgat aaaaaagatg
    12061 cctgagactt tcgtcacatt gttgaccact cggcgtcctt caaacaggac ttctggcaat
    12121 gtagtaaaat cagagtccaa caataccaga ttcgcaatct gacgtgttgc tccatcacct
    12181 tctgccatag caatgctgca atctgcttct cttaacgcca aaacatcatt cacaccgtcg
    12241 cctgtcattg caaccgttct gccgctttct ttcaattcat ttaccagcaa tttcttttgc
    12301 tgcggactca ctcgtccaaa aaccgtatag cgatgagcag cttctcgcac ttccatttct
    12361 tcagtaattt gagaaagatc gacatacgca tcataattcg gtaatcctgc acgtctagcg
    12421 atattggaga cagtaaaagg attgtctcca gaaatcactt tcagatccac accttcctca
    12481 tgaagataag ctagtgtttc ttgagcgttt tttcggattg gatcatcgat ttcaaacaga
    12541 gcaaccggct caacaaatgc tggtttttct tcgttcaatt gctgatttgg agcgatcccc
    12601 aacataagta cgcgatagcc attcgcttgg gctgttttta gactgtccgg aagccgatct
    12661 tcttccacca aacgttcagg cgctcctacg tacacgactc ccagttctgg aaattccatc
    12721 gcgccccatt tgcgatcaga agaaaatgcg actttgtttg tcacatcata ccgattagaa
    12781 agctcaaaat aatctctcat agcccgcata gtgatgttgt tatcactgct ttctgccaga
    12841 tacgtcccca taatttctgg gatggctttc tcgtacagcg gatgtagtgc ttctacccgc
    12901 tggatactca ttttcccttc agtaatggtc cctgttttat ccaagcataa tgtatcgaca
    12961 tgggccagcg tttcaattga atacatgtct tgaacaagga ttcgtttttt tgccagtttg
    13021 gttacccctg tcgttaatgc aatacttatt agcaatacca aaccctttgg aagcatgcct
    13081 aacaaagctg ctgcagatgc aataacagaa attttgatag aagcatcgcg gatgaacagt
    13141 gcttccaaaa atagtatgat ccccaaagga ataatcacga aactagtgaa tttggatact
    13201 ttgcgaatcg aatctacaag ttcagaacga atagctttgt gagtctttgc ctcgctagtc
    13261 aagcggacag cataattatc tgcacctact cggataactt cagccatgac agaaccgctt
    13321 gtcaaaaagc ttccagaaag caattcagct ccagtttctt tgacaatcaa atcagattcg
    13381 cctgtcaaaa gagcttcatt tgcttctgcc cgtccagata taacctgtaa atcggctggt
    13441 atctgttctc ctgctccgat ttttaccaaa tctcctaata ccagctcttt agtagaaatc
    13501 gattgttctt taccgtcccg gatcactagt acttctgatt gggaaacaat cgataatttc
    13561 cgtacaagat ttcttgcatg tatttcttga taaattccaa taacgatatt gacgacaata
    13621 attgctaaat acgccatgtt ggaatacgca cctacaaata acagacaaat cccaatacca
    13681 aagttcagaa aattgaataa cgtcaatata ttatcgctga atatttgttt tgtacttttt
    13741 gatgcatctt cctggtagtc attttgctgg ccagaagcca ttttctgaac tacctcttgc
    13801 tgtgtaagtc cattcatttt ctttttctcc attttccgcc aactttcaaa aataatcacg
    13861 tgtttaagtc tagcgtgttc caagattaaa aacaacttgt ttttataaaa tttatagaaa
    13921 aacacacgaa tgattttaca aaaaagatgc caaccttttt gtttgaatca cccgtgtgct
    13981 gtttttatta tcaatctatc tttacttata ttgttcctcc aaacggctca tccaccaacc
    14041 tagtgctgta ccggctataa aaagaacagg acaagctagt accccagcaa ctcccaatcc
    14101 cgcgtaatcg gtaggaatga tcataatcgt tgaagcaaaa actacaccta aaatgaaatg
    14161 gaatagcttg ctatatgcct ttgagaaaat gtaatccatg atttttgaca atccaagaac
    14221 acaaagaatc ccaccaattc cgatcggcac aatcacttca agtcgtactt ctttaaatcc
    14281 gtcagccatc gctttataca atcccatata caccaaaaaa ttagaagggc tcaatcctgg
    14341 gacgatcata cccaaaccaa tcaatccgcc ggcaatcagc catgttccaa aattctgatc
    14401 gacttgcccg tcaaataatt gagcgccgta taataaaaac aaataagcca gaacaaaagt
    14461 aacaaccatg atgatcacat cagaagtatc tcttcctttt ttccctgctt ctttccataa
    14521 cgcaggaaca gttccagcga tacatccgac aaaaaaccat aatatcgttg tttcatacgc
    14581 acctaataaa aaactgacta aaaaagaaag tagaaagatg cctgtcactc cgcctagacc
    14641 aacaggcaga aaaaacaaaa cattttcttt gaaatttttt gtaaggtggg ctaaaaaaga
    14701 aatcagccgc tcatacatgc caaagacagc tgctaatgca ccaccgctga ctcctggcaa
    14761 gataaatcct gaaccgataa acattccctt cacaaaccgt aacatccagt ctttggttgt
    14821 gtaccccact tcttgatttt ccattttttg ccttttttcc actatttgcg tactccttct
    14881 ttcatttaat tttattcatg attgaacatt tcctgctatt ttacttaaag actttttcat
    14941 aggtctctat tattttttca ttcattttga caatatcagg ataaaacatg ttacctccat
    15001 tatgatagag atagccgcca ttgtaaagca aggactggat ccgccaatat cggtattgtt
    15061 tcccttcctc atttcctaaa agtgggctca gcacatcttt agaatacgct tctgctaatt
    15121 ctactgtgtt ttctccgcca tgatttttta cataggcaat ataatctttc ccaaaattat
    15181 atgcttggac agctgtccat aaatcgcatc cttgtttctt tgcttctact atcatttccg
    15241 ccaaatattt gacaccttga tcaatactgt cagctggatt atcaatgatc tcagcttctc
    15301 catgcagact ttccgagctc tgcatgagat ctgtcccttt tccttttgat tcggtcaata
    15361 ttatcgcctt gatcaaattc tcgtagtttt ccatatgata attttttgca gctgttgtta
    15421 tttctatttg atagctttcc actcgtttta ccgaacgata agttcttcct cataaaaaca
    15481 acaccgcacc aataaccgtc agaatgatca tgtaaaaaag aatacggata atagataatc
    15541 gttttcgctt catttcatca cctagttatt ttgacatctt tttttatctt actatttctt
    15601 tatcttcttg gctatttctt ccttgatttc tgtgtacgcc ttaagaaaaa atgaattttc
    15661 ctgtattttt tgacatctta cgaaaatgat cgttgttttt cagtaaaaac aattgcccaa
    15721 tgactgatat aatcgttata ctttacacag taggtgatta tgtgaaaaaa gaaaagaaat
    15781 atgcaaaaat gccagatggg agcgaaattt actatgaaaa gagcggtcaa ggcttccctc
    15841 tctttttact gcatggcaat gacggcagcg gccgcttttt ttcagaacag gtccctgtac
    15901 tcgaacgcta ctataccgtg tatctagtag atagccgagg acatggtcgt tcgacaaacg
    15961 aagcatctat gttgaatttt cagttaatgg ccgaggatct gaatacgatc atgttgctgg
    16021 aaaaaatcga tcaggcagat tttcttggat ttagtgatgg agcaaatcta gcactggtat
    16081 ttgccagcag cttccctaaa aaagttcatc gattgatttt aaattctgga aacacgcttg
    16141 ttaaaggcgt tcgtttttct gccagagtca tcagtaatat tcattatgca tgggtatggt
    16201 tactctcttt atttcgccca agtcttcgca aaaatctttt agttatcaaa cttttgctgc
    16261 atgacatcgg gcttacagaa aatgatttaa aaaaaatcaa ctcccccact ttgatcatcg
    16321 tgggaaaaaa agatgtaatc aagctaaaac attctttgta tatagctaaa actataccaa
    16381 aagcttcctt tgtgcttgtg aaagaacaag gacatgaatt agctagaaaa gatcctgaac
    16441 gcttcaatcg agaagttctt caatttcttt ctgaaacata actattagga atagaaggtg
    16501 atttatttgt taaaaaatac catacaatgg ctgaaaaacc acctaggact gttcaaaaca
    16561 atctttttga tttctgtcat tgtcattatt gtcggtgaat tgatgtccat cggtaaaact
    16621 ctatctatcc agcaattagg agaaacgttt gccactatac ctgtatggaa aagcggacta
    16681 atgctattga ttggtcttat ctctgtgtta cctatggttg gttatgatat tattttgaat
    16741 cggatgcttg aacaaaagca gaatcctcgt tacctgtttg agacaagctg gctgatcaat
    16801 acgatcaaca atattgccgg tttcggcggg tttgttagtg tggggcttcg ctctgaacta
    16861 tatggacaaa agaaagagag cagaaacgtg atccaagcat tatcgaaagt tttcttgttt
    16921 ttgatggctg gactttctat ttacagtctg ctttcttttt tacttgtagt cttcacgcca
    16981 gttgctcctt ttttgaagca atattggata tggttgatcg gcggcggtct atattttcct
    17041 gtcgtctttt tatttactac tttcaaaaag aaaggattcc ttggtggtac ctcacttaaa
    17101 actcaaggac agctattgct agtttcattg ttggaatgga ccggtgtcct gtttagtttc
    17161 ctttctgttg gttatttaat ggaaatccgg attgatctgt ggcaaacaat tccattattc
    17221 attgctgctt cagtgatagg aatcgtgtct atgattccag gagaaattgg gagtttcgat
    17281 gtaatgatga ttatcgggtt gtccgctatt ggtgtgccaa gagaaatagt cgttgtgtgg
    17341 atcttgctct atcgcctgtt ctactacatc attccgtttt tgattggaat cgtctttttc
    17401 ttcaaaaata tcggttccac ttttgatcag cgttattctg gtatccctaa acaattagca
    17461 acagaaattg cgcataaaat cgtggttgtc ttattatact tttctggaat catgctagta
    17521 ctttctgcca ctatcccgca agcttttaca gagtttcgct ggcttcatag tttgaaccca
    17581 ttgaaatttc attttattat ccaatttcct agtatactat tgggatttct tttaatcatt
    17641 atgggcagag ggatcgcagc acgtgtgaaa cgagcatatt tgcctacaat cttcttgatt
    17701 gttctagctt tactttatgt tttattaagt gattttagtt tcacaccggt ggttttcttg
    17761 tctattctgc ttctgatcat tcttgcttct aaaaacgaat tatttcggga acaattgatc
    17821 tattcatggg agtggcgtac gatcgatgga atcattattg gtgcactgtc cttgctttac
    17881 atcgttattg gggtatacaa tctgccggat ttccctcatc ggcgtcacca ttttatttcc
    17941 tttttcttat ttccttctga aaagatttgg ttttcgggat tgttggccat catcgctgtt
    18001 agtttcatga tcgttctatt tgttcatttc cttcaaggag aaaagaaaca gattggcgaa
    18061 gcctttaatg aagaaaaggc cctgaagatc cttacaactt atggaggaaa ctccgatagt
    18121 caattgatct tcttaaaaga taagcggatg tttgcctatg aaaaagacgg tgaaccaact
    18181 gttcttctcc aattcgcttg cttcaataat aaatgtattg taatgggtga tccttctggt
    18241 aagaaagaag attttccaga agcaatcgaa gcttttatcg aagaaacaga tcgactatgc
    18301 tatttaccag ttttctacga aacaagcgaa gagattgtta tgattttgca tgaattcggc
    18361 tacgatttca ttaagatggg ggaagaagct tatgtagacc tgaatagttt tacaacttca
    18421 gggaaaaaaa tgaagggcac tcgtgctgtc ttgaatcgga tcgaacgaga aggatttaca
    18481 tttgatgtct tacaaccacc attttctact gagcaaatga gtactttcaa aaatatctct
    18541 gataattggt tgggttcacg aaaggaaaaa gggttttctt taggcttttt ctctgaagat
    18601 tatctacaaa gagcgcctat tgcagttgtc aaaaataccc aagaagaaat tgtcgctttt
    18661 gcaacgatca tgcctactta cacgaacaat aaagtaggaa cgattgatct gatgcgatat
    18721 gattctaaaa aagcaccttc tggcagtatg gatttcttgt tcttgcattt gtttgcctat
    18781 atgaaagaag aacatatcca atggttcaat ttagggatgg cccctctttc aaatgtcgga
    18841 acttccagaa agagtttttt acaggaaagg attgcatact tggtttacga gtttggttct
    18901 catttttatt ctttccatgg attaagagaa tataaaagca agtacgctac aaactgggtc
    18961 tcacgttata ctctatattc tagagatagt tggattgcat atgttatgat tgctttgctg
    19021 attattgata atgcgccagt tgagcagaca caaggtagaa taagtggttt gaaaaaatgg
    19081 atacgaagaa agtattaaaa actgcatacc gatagatgaa cactctcatg atgaataaaa
    19141 gagactgaag taaaacgaaa gttcatgctt ctacaatctc ttcttataca attgaagaac
    19201 aaagagcggt ccaaacagaa atcctgtttg gaccgctctt ttattttaaa catttttcat
    19261 taccaaaata ttcgagtcgc gtttcttatg cttcgatctc tgtaacgata cctgaaccta
    19321 ctgtccgtcc gccttcacga attgagaatg tcgttccgtt ttcaacagcg attggatgga
    19381 tcaagtcaac atcaatcgtc acgttgtcac caggcatgac catttctgta tcttctggca
    19441 atgtgattgt tcctgtaaca tcagttgtgc ggaagtagaa ctgtggacga tagttgttga
    19501 agaatggagt atgacgtcca ccttcttctt ttgtcaacac gtacacttct gctttgaatt
    19561 ttgtatgtgg agtaatggaa cctggtttag caagtacttg tccacgttcg atatcgtcac
    19621 gttggatccc ccgtaacaac acgcctacgt tatccccagc ttccccgtaa tctaacgttt
    19681 tacggaacat ttctacacct gttacaactg ctttttgtgt ttcaggtttg atccctacga
    19741 tctctacctc atcaccgaca cgaacagcac cacgatcaat acgacctgag gcaacagttc
    19801 ctcgacctgt aattgagaag acgtcttcca ctggcaatag taatggttta tctgtatcac
    19861 gttctggtgt tgggatatat tcatctactg tatccatcaa ttccataata gcagcttcag
    19921 catctggatc gccttgcaat gcttttaatg cggaaccttt gatcacagga gtgtcatcgc
    19981 ctgggaaacc atattcgctc aataactcgc gaacttccat ttctaccaaa tcgatcaatt
    20041 cttcatcatc gactaaatca actttgttca agaaaacaat caggtatttt acaccaactt
    20101 ggcgtgacaa taaaatgtgt tcacgtgttt gaggcatcgg accgtcagtt gcagaaacaa
    20161 ctaagattgc accatccatt tgtgctgccc ctgtaatcat gtttttcaca tagtccgcgt
    20221 gtcctggtgc atcaatatgc gcgtagtggc gtttttctgt ttcatattct acgtgtgcgg
    20281 tattgatcgt aatcccacgt tcacgttctt ctggagctgc atcaatacta gcatagtctt
    20341 gaggatttgc taggcctttt ttgcctaata ctgtagtgat cgcagcagtt aaagtagttt
    20401 tcccatggtc aacgtgtcca atcgtaccaa tgttaacgtg tggtttactt ctgtcataat
    20461 gttcttttgc cataatttac aaacctccta aataatatag atcactgaaa aatttcagct
    20521 ctcacctttt tattatagtc tttttttgac taaatcaaaa agaatacgtc cgacttactt
    20581 tttgtaagat tcgaactaag cttattatac gtcaatattg gtcaaagtca actaagagca
    20641 ctcgtctgtc ttatacgcga aaaaagtacc ttttataaat aaaaaaatgt gttttttgag
    20701 aaaaacttca agatagtcct taactttctt ttgaagcaat aagaacaaaa tgaggaaaag
    20761 tttaaaattc agctgttgcc aacaactttc aatagacgaa atcttctatt tcgttaataa
    20821 taaggaaaga ggtgaagtac atgaaaaaat taattggcgt acttgtcgtt ttaggcatat
    20881 ttctcggcgg aagttttgca gcttatcatt atttttacgg cggcgaagct tattatacaa
    20941 aaatcacgac gaatggcgaa atgtcagcta cccaagcaga taacggagag aaattcacga
    21001 cttataccta caagcaagcg gcttataata aagatggtga aaaaaaacag gtgacgcttc
    21061 gcgaagaaag agaacatcca ttaaagatga atgcgtatct taaactcaaa gtcaatcctc
    21121 gtaaaggtgt attaagctgg gaagaaataa aggcttctga agtgcctaaa aatgcggcag
    21181 aaaaaatcga taattagtaa atggtcttaa aattttatta gataaaaaga ggctgggaca
    21241 gaagcgttta actccaagaa ataagaagga gttgcttctg cttccgccgt ttatacgtgt
    21301 ttagagcgtg agacaaaagt gttctttact tttgtctcac gctctttttt tgttatattc
    21361 gtcttttccg atccttctct tgacacaatt catttaactg atcactggta cttgaacacc
    21421 taatattcta gtcattttta taaacgattc aatacttcat ccacattttt gatcatcacg
    21481 gatatcgtac catccggatt attcacaaat tcaatcaaat ctgggttgcg atatacatct
    21541 actggtacga tcagttcgat cccgtttgat agcttgaatt tttgttttcc aaattttttc
    21601 tcagaaattt cttgtacttc ttttacagga ggtgcttctg ggacaaatcc ttgctcttgt
    21661 acttcttctt taaaagccat ttttgccgtg atgttttcct tgaagacttt ttccgcaatc
    21721 atctcatgat ccaaccgacc tttttcttct atcgtatcat agacagcttc tttcaaatca
    21781 gccatgatat caaattcttc tgtctcaaat ttttttccaa gatgcttggc tactttttgg
    21841 atcactttta cattttcttc taaagaaggt gcaggaacac tttcgatcac tcttccagaa
    21901 aaataaagct cttttttgcc agaaaactca tatttttttt caattaattc gtaggtccaa
    21961 tcagtcaaat tcacagcgaa tgcttcatct gctttttgtg ttttcgcccc tagaattgct
    22021 cgattcaaaa tcaagcggtt gtcgatccct gcttcgtctg cttcaacaaa gtgagtgtaa
    22081 gcctcacgat aattgacttt caataaagat aagtacatca ctgtatctaa ttcatacaag
    22141 acaacaaata catctgcact aggtgcttct tcactttctt ggtagatatc gaaccaccgc
    22201 tgaacgattg cttcacttgc ttccacaaaa ttttcttgag cgattttaag tgattcaacg
    22261 atcatgcttt cttctgctag ttgtccagtt tttgtttgag cagaagagat tttttggatc
    22321 ttcttttgaa gaaaatcacg aatatattct tttgtcagat ccagctcttt ttgtgaaaag
    22381 actggagaac cagtttctct gtcaataata tgcagaattg ctttttttaa atagatatcc
    22441 attttctcat tcccttctgt cttagtttac ggaaaaaaaa tcaaaaatac cagaatcaaa
    22501 atcattttct tttaaacaaa aatttcaaga gtagaaacaa aatggattct ttttttcgaa
    22561 aaatgcgcta cacttatagt tgaacgaata aaaaaggagc tgaaagtatg aattcattaa
    22621 caagcactta tcgtctatca aatggttacg aaatcccggt agtaggattc ggcacatggc
    22681 aaacacctga tggggatgtc gctgtttcct ctgttaaaga agcgctggct gcaggttatc
    22741 gccatatcga tactgcccaa ggatacaaga atgaagaaag cgtcggccaa gcgattaagg
    22801 aaagtggaat tcctagagaa gagatattct tgacaacaaa attatggaat gcaaaccaca
    22861 gctatgaact tgtgatgtct tcttttgaag aatcattgaa gaagctccaa acagattatt
    22921 tagatctgtt tttgatccac tggccaaacc ctgttgcttt tcgtgataat tgggaacaag
    22981 caaatgcaga tacatggcga gcatttgaag aactgtatga agcaggtaaa atcaaagcaa
    23041 tcggtgtgag caatttctta ccccaccatc tagatacact agcaaaaaca gcaaaaatta
    23101 tgccgatggt caatcaagtt ttccttgcac caggcgaatt acagacaact gtagttgagt
    23161 atgcaaaaaa acatgatatg atcctcgaag catatagtcc attaggcact ggtaaaatct
    23221 tcgatgtgcc tgaaatgaaa caaatcgctg aagcacatga caagacgatt gcacaagttg
    23281 ctttgcgttg gtcgctgcaa cacgaattcc ttcctcttcc taagtcagtg acaccaagca
    23341 gaatcaaaga aaatactgaa ttatttgatt ttgaattaac agaagaagaa atgaaacaaa
    23401 tcgatcagtt agatggtgta gtcggcaaag caaaagaccc agatactacc caattttaag
    23461 tcgaaaggtg attttatgga tccaatcgaa cgcttgaatt ctctctcgga agaagttacc
    23521 cagacttttc attccgattt tgtctttttg atcgatgcag agaaaatcca acattttcct
    23581 gcaagaaact ggacacatga tcaaatcatc gaagaactga aaaaacggtt cgatcattcg
    23641 ctaatggtca aaccctggca cgaacatgaa gtcatttatt cacctgaact acctgttttt
    23701 gccttgatac cgaaaaaata gaaagaatgg aacggattat gtatatatac ataatccgtt
    23761 ccatttttta tttttctttc aaaaaacggg aaaatacgca aatttttctg ttgtaatttg
    23821 cgtatacgca agtttttttg cattactcct tttcatttgg ctgttttttt cttataatcg
    23881 aaagggtatg agcgaaggag agaacatatg aagaaaaaac agaaacaaaa attattaaat
    23941 caattccgtc cttcattgga tggtgtaaga ggacaattat tccgttctct agaagaagag
    24001 agcttgcgac gttatggttt gccgttacaa gtcatgcttg atcccaaaaa ccgtcaaatc
    24061 ttaacgatcg gattagctgg gaaaacaaat gaagatacac ggatcaacgt gccaatcgat
    24121 gacaacttca atactgtatt aaagagaatt cgttcaggag aaaacgggat tttcgaacga
    24181 ttccgtgaca atcttcttat tgaaatcgtg tcttactgga atgatcaacg attgaaaaac
    24241 gatgaaccaa ctgcgcaagc tgttgcgaca ccagttacag aagaaacagt cagtgtgcca
    24301 gaagaaaaaa cggaaaaacc aaaagcggac acaactgaaa atacacgctc aactgatacg
    24361 aatgccgctt taagttttgc tgatttttca aaagccgttg ctgagtatcc taaattttac
    24421 acagaacaaa atgaacatga agtagttatt aatgaaaaaa atgctgatga acctcgtaaa
    24481 ttagcaacta tttctatgac tactgaaaat gagttcgtta tcgaaaaagc actcgaacgg
    24541 aaatacaaag tcaaattgac tttgattcct ttgatcgaac aatttgcagc gacagcgatt
    24601 actgatcgat aaaaattcta tttgtcataa aaggctggga tttaaaatcc cagccttttt
    24661 agtcatataa taatttctac agcaagtaaa aagaaattcc ttctaacatc aggctcaaat
    24721 atcagcttga cctgtccaac aaatcaccta tgatcctaca atgttaggcg acttctcttc
    24781 tacctgaaga tatcttggag ttgcctgatt tcttaagatg cctaacctta actataaaaa
    24841 attctaataa taaacatatt ttctgataga gaaaaaa
//
LOCUS       DAYYCW010000032        24734 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000032 DAYYCW010000000
VERSION     DAYYCW010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.032"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..336)
                     /locus_tag="ACHAYA_001208"
     CDS             complement(<1..336)
                     /locus_tag="ACHAYA_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8605016.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLE"
     gene            complement(466..1050)
                     /locus_tag="ACHAYA_001209"
     CDS             complement(466..1050)
                     /locus_tag="ACHAYA_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8605017.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(1381..1614)
                     /locus_tag="ACHAYA_001210"
     CDS             complement(1381..1614)
                     /locus_tag="ACHAYA_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8605018.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(1629..2579)
                     /locus_tag="ACHAYA_001211"
     CDS             complement(1629..2579)
                     /locus_tag="ACHAYA_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM8605019.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(2576..3418)
                     /locus_tag="ACHAYA_001212"
     CDS             complement(2576..3418)
                     /locus_tag="ACHAYA_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM8605020.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(3420..4139)
                     /locus_tag="ACHAYA_001213"
     CDS             complement(3420..4139)
                     /locus_tag="ACHAYA_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8605021.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            4687..4989
                     /locus_tag="ACHAYA_001214"
     CDS             4687..4989
                     /locus_tag="ACHAYA_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8605022.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            5061..6287
                     /locus_tag="ACHAYA_001215"
     CDS             5061..6287
                     /locus_tag="ACHAYA_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8605023.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(6375..6767)
                     /gene="rpsI"
                     /locus_tag="ACHAYA_001216"
     CDS             complement(6375..6767)
                     /gene="rpsI"
                     /locus_tag="ACHAYA_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8605024.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(6783..7211)
                     /gene="rplM"
                     /locus_tag="ACHAYA_001217"
     CDS             complement(6783..7211)
                     /gene="rplM"
                     /locus_tag="ACHAYA_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM8605025.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(7745..8212)
                     /locus_tag="ACHAYA_001218"
     CDS             complement(7745..8212)
                     /locus_tag="ACHAYA_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM8605026.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(8332..9870)
                     /locus_tag="ACHAYA_001219"
     CDS             complement(8332..9870)
                     /locus_tag="ACHAYA_001219"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM8605027.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            9950..10636
                     /locus_tag="ACHAYA_001220"
     CDS             9950..10636
                     /locus_tag="ACHAYA_001220"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM8605028.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(10837..11364)
                     /gene="msrA"
                     /locus_tag="ACHAYA_001221"
     CDS             complement(10837..11364)
                     /gene="msrA"
                     /locus_tag="ACHAYA_001221"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8605029.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(11448..12125)
                     /gene="pgmB"
                     /locus_tag="ACHAYA_001222"
     CDS             complement(11448..12125)
                     /gene="pgmB"
                     /locus_tag="ACHAYA_001222"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8605030.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(12122..13252)
                     /locus_tag="ACHAYA_001223"
     CDS             complement(12122..13252)
                     /locus_tag="ACHAYA_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8605031.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(13252..15435)
                     /locus_tag="ACHAYA_001224"
     CDS             complement(13252..15435)
                     /locus_tag="ACHAYA_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM8605032.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(15432..16457)
                     /locus_tag="ACHAYA_001225"
     CDS             complement(15432..16457)
                     /locus_tag="ACHAYA_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8605033.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(16502..17329)
                     /locus_tag="ACHAYA_001226"
     CDS             complement(16502..17329)
                     /locus_tag="ACHAYA_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8605034.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(17326..18393)
                     /locus_tag="ACHAYA_001227"
     CDS             complement(17326..18393)
                     /locus_tag="ACHAYA_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM8605035.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(18419..19255)
                     /locus_tag="ACHAYA_001228"
     CDS             complement(18419..19255)
                     /locus_tag="ACHAYA_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8605036.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(19252..20121)
                     /locus_tag="ACHAYA_001229"
     CDS             complement(19252..20121)
                     /locus_tag="ACHAYA_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8605037.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(20191..21474)
                     /locus_tag="ACHAYA_001230"
     CDS             complement(20191..21474)
                     /locus_tag="ACHAYA_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8605038.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(21488..23173)
                     /locus_tag="ACHAYA_001231"
     CDS             complement(21488..23173)
                     /locus_tag="ACHAYA_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8605039.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            23448..24473
                     /locus_tag="ACHAYA_001232"
     CDS             23448..24473
                     /locus_tag="ACHAYA_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8605040.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         7370 a         5049 c         4206 g         8109 t
ORIGIN      
        1 ttctaaagag gataataatt cattgctagt atagacacta accaacaacc atgagatcat
       61 tacgaccggc aacattcgaa atacaatata taaggatgtc gcaaaaaata tagtaaaaaa
      121 ctctatcttg atagcatcaa taattttcat tactaaaaac agaactgaat aaatcaagat
      181 gaaataaata catgtcttta ttttacgaaa catcaatact aaaactgtag caataatgaa
      241 acaccccact tgaaatatat ttccctttcc taaaaaaata atgaccggaa aaaaaatatt
      301 taaagtaatc aaggttaacg gattgatttt tgacactctt tcacctccaa aaaataagat
      361 cgcactgtta tcttaagctg ataaaaatgc accgttattc agataacagt gcgatcttat
      421 caaaaagcat aactgagaac aattctcaat gcaagctttt tttaattaac tcagtaattc
      481 aatccaccca ggaacagaaa attgatacaa tcttctggtt aatagcatag tcttttcttt
      541 tgaatagcta tgcttcaata tatcatagaa tgtagcgtag aatgagtaaa tgtagaagtg
      601 gatttcttct tcattaaaga ctaaattttg attgatcatt ccgatagtct ttaacttatc
      661 tagacaacag actgtctttt tcgtcacaaa tgatgtaatt ttttcaacaa tatcttgata
      721 tttggttcca gctgattgga ataaaatcaa atcgaatata cgtttattct caaaaatata
      781 attaataaat ggatctaatt cattgaagtt ctctctccaa cttttctcta tatctatatt
      841 ttctaagttt aagaggtatt tggtaaactc ttcattatac atattaataa gcgcattaat
      901 atgcggctga ataatttctt caaataacgc ttccttagaa gtaaaatgtt tataaaaagc
      961 acccgtagtt acgcctgcgg tcaggcacag ttgccttatc ttagtttgct gaaatccttc
     1021 tttttcaaaa tggcttagtc cactttctat taacttttgg tgagttataa tataatctct
     1081 ggtttctatg ggtacttcct cctaataaga tgaattaact ttattatctt acacaggact
     1141 tgaagtttca atcgttaacg tttattactg aactcattcc ttagagctaa actaatgaga
     1201 aagagcagaa taactgatac aaaaagcaat cctacaagaa ttaatagact catctaaatc
     1261 attcaccttt tctaaaataa tccgagaaag atttaaaaaa attatatcca gctattagga
     1321 taagtcctat cataatcact ccaatagcca ttttcttatc ctccctaaaa ataaagtctt
     1381 ttacttaaaa ccttttttgt aatcgtcaat aaattttttc cctttatcag ttaattgaaa
     1441 tcctatatct aaatagtcct catatctaag tttagccagc atctcactta cagatgctgg
     1501 actaacactt agaacttttg ctaaatcatt attttttaat ttcttttttc tttttgataa
     1561 ttcctgaatt gctagtaaat aaattagtat agtagtcgtt ttaatttgta ccatacctac
     1621 cctccaaatt atttcgaaag gccttcagca attttatcca ggttccattt catcatcgca
     1681 tagtaactat caccatcttg tcctttttct gcaattgaat cagtaaaaat cgttgaatag
     1741 ataggaatat tggtatcttt tgatactgtt ttcattggtc tatcatccac actgctttct
     1801 acgaataagg agggaacttt tgttgtgcgt aatttttcaa ctaagtgttt tatttgatct
     1861 ggtgttcctt cttcttcagt gttgatttcc caaatgtaag cagaaggcac attatacgct
     1921 ttcgagaaat atttaaagca tccttcactt gtaacaatca tttttttatc attcggaatt
     1981 gaagcaaatt tagatttagc ttctttgtct agagaatcca atttttcaat atacttatct
     2041 agattttctt tatagaattt tttattatca ggatcttttt ccgctaactg tttttcaata
     2101 tttttagcgt aaataatacc gttttctaaa tttaaccaag catgaggatc ttccttccct
     2161 ttctcactct gaccctctaa gtaaataaca tctatgccat cacttgctgc aaaatagtct
     2221 ttgttttcct ctttgttcgc atttttaact aattttgtaa accaagcatt tcctccagtt
     2281 tccaagttaa caccgttata aaaaatcaaa tctgcttttg aagttttttg aacatcttca
     2341 ggcaaaggtt catattcgtg gggatctttc ccaataggta cgatactgtg taaatcaatt
     2401 ttatcttttg ctatattttc ggtaatatct gctaaaatcg aattagtaac tactactttt
     2461 aatttttcgt tcgaagccac tttatctttg tctttactat ttattgaaca tgccccgaat
     2521 ataaaaagag caatcaaaat ccccagactt cccagtactg ctttccattt tctcatcata
     2581 acgatctctc cttctttctt aaaaattttt gttttggtga gattagaaaa ctaattacaa
     2641 ataagaatgc cgctgttaaa acaatacttg atcctgctgc tatgttaaaa ctataaccaa
     2701 taaacaatcc aagcaatgat gaacttgctc cgactgttgc agaaataaat atcatagttt
     2761 tcaagctatt tgcatataaa tatgctgtag ctgctggtgt aattaacatt gcaacaatta
     2821 atatagttcc cacactttgc atagctgtta ctgctactaa agttaataga accatcaata
     2881 gataatgata aaaactgaca ttcatcccta ttgcttttgc cattaatgga tcaaaagaag
     2941 tgattaataa ttctttaaaa aatagaacta ctataaccaa cactaaaacc gaaacgccaa
     3001 ttgtaatcca cttatcaata tcttgtacag ctaaaatatt tccaaataaa atatgaaata
     3061 aatccgttga gctatttgca actcctataa ggataactcc tagtgctaaa aaagaactga
     3121 aagtaatacc aattgctgta tctcctttta ttacactatt gctttttata aaagtaataa
     3181 tgatagaagc taatagacca aatacaatcg caccaataaa aaaattaata cctaaaatat
     3241 aagaaattgc tactcctggc aaaacagcat gagaaatagc atcacccata agagacattc
     3301 ctcttaaaat aataaaacaa cctactgttc cagaaacaat ccctataact acagatgtaa
     3361 ttagagcatt ctgtaaaaag tgaaagtcat acagaccatt aataaaattt tgaagcatat
     3421 tattccccct ctccaataaa tatagtatct ccataggctt tttttaaatt ttcttcatta
     3481 aatacagatt ctacacttcc gtgagcaatt agatttcgat tcaaaagaat aatattatca
     3541 aaatacttct ttaccttgct taaatcatga tgcacaatta gcactgtttt tccctgatgt
     3601 ttcaattttt ttaacgtatc cataattatt cgttcactaa ctaagtctat ccctacaaat
     3661 ggttcatcta aaaatatgaa atccgcattt tgagcaagac atcttgctaa aagtacacgt
     3721 tgaaattgtc ctccagataa ttctcctatt tgatgattag aatattttcc catatcgacc
     3781 ttttccaaag cctttgaaac tctttgccat tctgcatttt taattctttg aaaaactttc
     3841 attccggcat atgttcccat agatacacat tcttttactt ttattggaaa agtaaaatca
     3901 atatcagctt tttgctctac atatgcaatc ttcaccagtt ttttattgat atcttcatta
     3961 ttaatggaga cctctccctg atgagggata atatttaaca ttgctttaag caatgtggat
     4021 tttccagcac catttggacc aataatacct gttaaagcac cttcttgtat ttttaaagaa
     4081 atctcgttca atgctaaaaa atcgttataa caaacattaa gctttttcac ttctaacata
     4141 actttctcct tctaaatgat aatgattatc acgatcatat ggtatacaaa aattatattt
     4201 tagtcaaccc taaattttat agtttagatt tttttctgtt gctgaagatt agaatgacaa
     4261 aactatttga aactacgcgt atagtaagta gctttcaaaa tatataaaaa aggagcgttc
     4321 tcaaatgaga acatgctcct aaaactactt tattggcgta tggaaaaatt atacttgaag
     4381 tggatagcgg gttgaataag ttgataagca tcaaaacatt tataaaaatt attcaagact
     4441 atgtctgttc catatacaaa tatgaaaatc agtaaactcc atttaacttt tttcgtagtg
     4501 tgtcttttta tacatgttta tcgttattaa tagtgaatag gaaaaaaact atttttgatc
     4561 attgacaatt aaatacaatt tttgaataac gatccctgct attcgagcta gaaactcaag
     4621 tacgccatga cctgattagc gacaaatact tagagatttc tttttgttct gggaaaacaa
     4681 aatgccatga caatagcagc aaataatgat acttccgtaa ggctcgccgt aaactgggga
     4741 gaggtgaaat tcctatgtgc ttacccaaag caattcaaaa atttcataac tactatttta
     4801 ggagatgatt ttatagaatc cagagtctac ctgaataaaa aacaaacctg tgcatttcta
     4861 ggaatcgata ataatactct agataagtgg attcgtatat ataatttccc agtaataaga
     4921 gttggtggtg tagttcgctt tgacgtagaa gatattaaag actttatgaa tacatttaaa
     4981 ataacttgaa attttcttct cgccgcatat actaaaatta gtgaaatata ctgccttact
     5041 ttttttaagg agtgaggatt ttgcctacaa agctttcaaa tgggaaatat aaaactaatc
     5101 tgagatatcc aaaaaggttt aaagaaataa caggaattgc atcagaaaag tatcaaaaaa
     5161 catttccaaa taggcaattg gcgattaaag ccgaaaatga tatgaagaag aaaatagaaa
     5221 aagttttgag agaggagaat gcaaactcat tagaattaaa aggaaaaata acgtttaaaa
     5281 aattttacga atctaaatgg cttcctagat atgaattagg tcaaacaatt agatcaaaca
     5341 ggcctcctag tgatataacc attagtaata caaaagatat atttcggttg catattttac
     5401 ctatgtttgg tgaatacgca atgaattatc taaatatcaa tactgagata atatcagatg
     5461 aattaaccaa aaaatcgaaa gaatatgcaa atataaaaat cataaaagga tatgttcgtt
     5521 ccatgttcga tattgcagaa atactaaatt atatcgaatt taatcgaact actaaaatta
     5581 ttcaaagtat cactgcacct aaaaaaaatg ctttagaaga aaaaagaata caagaaggta
     5641 agcaagcact atcatcaaaa gaacttacta attggataga agcagtaaat gatgatttta
     5701 ataatcattt acttactttt catgattaca cactttttat gattaccctg tatcttggtg
     5761 atagaaaaag tgagacttac gctttgcaat ggaaatatat tgattttgaa aaacaaacgg
     5821 tccgtcttaa acatacttta gataaatatc aaagaaaaaa gtttacaaaa ggtcgaaaag
     5881 ataccgtcat tcaagtacca gaagttgtaa tgacattgct aagtgaatgg aaatcggtac
     5941 aagctgatca attgttgaaa ttaaaaatta agcaaacgtt agatcagtat ctctttactt
     6001 atactaaacc atctggagaa gtaaattgtc ctgttcatgc cgattaccta aactatcgaa
     6061 tcaattcaat taaaagaagg catcctgact tagttcattt atctccacac aaactccgtc
     6121 atacttatgc aactatagct agacaaggtg gagcagatat gaatcaaatt tcaaatgctt
     6181 taacacactc agatatttct acaacaaaga tatatgtgaa cacaccagat atagttgata
     6241 aggctgtttt tgaagctttt cagagaggat tgaataagtg tgattgatat gtgttcttaa
     6301 atgtgttctt aaaaacaaaa gaagcacttt ccaaataatt gaaaagtgcc agaaacgttg
     6361 atatataaag gatattaacg ttttgagaat tgtgaagctt tacgtgcttt cttaagacct
     6421 ggttttttac gttcgaccat acgtgcgtca cgagtaagta agccagcacg tttaagagct
     6481 aaacggaagt caggatcaac ttctagcaat gcacgagcga taccatgacg gatagctcct
     6541 gattgtccag cgtatcctcc gccgtttacg ttaacaaata cgtcatatgc gccttttgtt
     6601 tcagtaacag cgaaaggttg attgattact tcacgcaagt cagcatgtgg aatgtattct
     6661 tcaacatctt ttttatttac agtaattttg ccagttcctg gtactagacg tacgcgagca
     6721 actgcgtttt tacgacggcc tgtgccgata tattgaactt gtgccaatga aattccctcc
     6781 tattagatta agtttgtgat gtctaaaact tctggttgtt gtgctgcatg tggatgttct
     6841 gctccaccgt atacgttcaa ttttgtaaat tgtttgcggc ctaaagtgtt tttaggaagc
     6901 atacctttta cagaagtttc gatcaaacga cgagaatttt tagcacgcaa ttcgccagca
     6961 gtgattgatt tcaatcctcc tgggtagttg ctgtgacggt aataaatttt gtcagtcgct
     7021 tttttacctg ttaatttcac tttgtctgca ttgatgacga tgacgaaatc gccagtatcc
     7081 aaatgaggtg tgaaagttgg tttattttta ccacgtagta cagatgcaac aactgttgaa
     7141 agacgtccca atggaacatc agttgcgtct actacatacc atttacgttc tacttcgcct
     7201 ggtttggcca tatatgttgt acgcacgtta ttttcctcca atttaagttc tgatgtttga
     7261 catacacttc gagtttccgg ggctctttgt ggggcaaaca ataccattta acatattacc
     7321 cgctaatcta gtcaatgtca acgtttttct gcttgattga aaaatttata tgaaacttta
     7381 cttcttttct ttcttttata taaaagggtc gtttcctgtc aaaatgaaaa acaaaaagag
     7441 gctgtgacaa aagtcttgtc acaacccctt attttgaata aacggtgttc tcctgccaac
     7501 tcctcggtca caagtcacaa tatccataaa acataagaag ctgtttttta aaattgtttc
     7561 ttgtgactcg gagctgaacg gcaggcttac aacctcttgg taaacggtgt tcaatcagct
     7621 gaccctcggt attagctcaa aaaacggcaa aatatgagga actattttga cgtttgtttc
     7681 tctaatactc aggtcaaaac gctgatttca caacctcttt ttcggtcaag ttcaacaaca
     7741 gtttttacgc gtcgatctct ggcgctttgc ccaaatgagc ttggatcatc cagatacgtt
     7801 tttctgtggc tgttttgaat tcgataaaga tatcttgtgt tggatcgtcg ccttcttctc
     7861 cagaaacttc gattcctttt tggtaaagat ctgctagata acgatagcct gcaacaagtg
     7921 tttccaaacg ttctggaata gtacgatccc agcggccaat ttcatcaggg atttttgtgt
     7981 tatctgcaaa ttcacgcaat gtagaataag gtgaaccgtc taatgtgatc aagcgttcgg
     8041 atacttcatc taactgagcg ttcaaatcat ccatgaactc atccatcatt gggtgcaatg
     8101 taaggaaacc tggtcctctc atgtaccaat gtgtttggtg aacaacaaca gacatttggc
     8161 ttagatctgc aactaattga tttaatactt ctttggtttg ttggaatttc atctttcttg
     8221 cctcctaaca ttttcttaca cttaaaataa tagtagactt tcaatgaaaa gtacaacaaa
     8281 atcatttacc tgagaacgat tttcagttcg cttttttatc cgtaaagcca atcatttttc
     8341 agaatcagct aattcttctc gcagttgagc aaattgctga taagtctgtg ctaaatgctt
     8401 ttgtgtcgaa acaaataatg gatagatttt ctgatatttt ttatgatcag tttcttgtac
     8461 tccaacttgc tctttcaatt ctatcaattc ctgtgcttca ctgatacttt ggatctttcc
     8521 gatgcttctc cagcctaaaa tcacagctcc taatgcggat gcttctgtga cagtagggaa
     8581 aacaagcggg taacccagta catcagccat catctgacga aaaatcggag attgagcaaa
     8641 cccgcctgta gcacggatct ctgtgagctg attttcctgg caaactcctt ttaaaatccg
     8701 ataaaggttg agacagatcc cttctagaat cgccttcatc atttcttttt ctgtatgttg
     8761 ccgctgtaaa ccgataaaac tgccaaacgc atctgcgtcc caaagcggtg ctcgctcacc
     8821 aagtaaatag ggatgaaaaa tcagaccatt ggcacccgct ggcacaaaag cgatagccga
     8881 aagcagtgca tcatatggat tttctccttt ttctttagca gattgaacga cttcctgtaa
     8941 aaatgtatgc cctgcccaat caaagatgcc agcaccattg ctagtcgctc ctcccactac
     9001 ccaatgtttc tgatccagaa cataacaaaa cgtctccatt tctggatgga actgaggctg
     9061 agtagtaata aaacgaagtg ctccgctcgt tcctacggtc aaagcggcga tcgtatcacc
     9121 aaaagcacct aaccctagat tagaaagcgg cccgtctgcc cctcccaaaa caaatgatat
     9181 atcatttggc aaacctagtt ctgctgctgt ttttgatggt agacctgtta cttgataggt
     9241 ggaaggaaag acttgcggca attgctctat tcgaatgttc aagtagtcaa gcacttcttc
     9301 tgcccattgg aattcatgga tatcaaaata tccagttcct gaagctgttc cgtagtcgct
     9361 gatcagttcg ccaaacaatc tataaaagat atagtctttg ataccgacaa aataagcagt
     9421 ttttttgaat aattcaggtt ggctgttttg cagccacttg attttgttca atggtgtcat
     9481 tggatgaatc ggcgtcccag tctgccgata aaattctttg ccaagcacgg tatatttcag
     9541 tttttctgct tcgtccgctg ctcggttatc tgcccaagtg atcattcgag ataacggtgt
     9601 tttctcttca tccaacaata tcaggccatg catcgcagag gagaaggaga caaattgaat
     9661 gattgtgtct gaaggcaact gtttaattac ttcttttata acggtgcaga caccttgaaa
     9721 gatcaattcc tgatcttgtt ctgccatccc ttgtgtttct tggatcaaag ggtagcgaaa
     9781 ataagcggat gctgcttctt gggcttcatc tgtgaaagca actgctttgg tttgtgtggt
     9841 tccgatatct aatccgattg aaacgttcat tttttcacct caaattttat gtatgtacaa
     9901 aaatttagtt taagcttgcg tatcttggtt ttgaaaaggg ggagatggcg tgttacttta
     9961 ttttatcatt ggttgctgta tcggatcatt tttatgttta accgcccagc ggattccgtt
    10021 aggtcactct attatttatc ctagatctca ttgtgtcaaa tgctgtcgtt cgctttcttg
    10081 gtatgaattg attccgattc tctcgatcat cgtccaacga tttcgctgcc gctattgcag
    10141 gtgtcggttg cccttctact atttgttggc agaagcctta tgtggtggtc tttttgcctg
    10201 gttctttact ttttcttccg caagaaattt ttcgacgttc atatggatgc tttcggcttt
    10261 gctattttcg ttgatggatt tattttactt catggtccat tctcacacgc tattctgttc
    10321 ttgggcaatt ttatggattt tttggttgca ggccggcgtc ttccaatggc aaagtgtgct
    10381 gttaacgttt atcgtaggcg gtgtgtgcct tcgctatggt caatcctatc tcggagctgg
    10441 cgatactctg ttgatgcttt cttggagtgg tggcttgtct ctagaagagt tgcttcagat
    10501 cttgtttctt gctagtctgt ttggaatatc cgttttttct gtttacttta tctgtcataa
    10561 aaaaaagttg gaaaagctgc ctttcattcc ttttttgagc agtgcgttgc tgattgtcct
    10621 tcatcttagg tattgaaaaa aggttgtgac aaacgacttg tcacaacctc tgatatatag
    10681 tgttctctta ccaaatcctt gtgacttgga gctaggcgac aggttcacaa cctctccact
    10741 aaatggtgtt caatcagctg accctcagta ttagctcaaa atgctggttt tacaacctct
    10801 gatatacggt gttccataag tagctttttc ggcttcttat ttcttgacgc cccaatgtcg
    10861 acgaaggaaa cctgcacgtc cagaaccttc tcgataaaga ttgtaatgga gcttgttttt
    10921 cttgtaataa tcctgatggt aatcttctgc aggataaaat tcgcttcttg gttcgatttt
    10981 tgtcacgatt ggcttatcga atcgcccgct gtcttgcaat gcttgttttg aacgctctgc
    11041 aatatttcgt tcttcttcat cattatagta gatcactgga cgataagaat caccgcgatc
    11101 agcgaactgt cctaatgcgt cagtcggatc ggtttgctgc caatagattt ccaccaactg
    11161 atcataagaa ataacttctg gatcatattc gatctctact gcttctgtat gccctgttgt
    11221 gcctgttgtt acttgttcat aagttggatt tggtacatgc ccgccagtat aaccggatgt
    11281 cacagaaatg atccctggct gctcttcaaa tggtttgacc atacaccaaa agcatccgcc
    11341 tgcgaaaact gctttttttg tcatataaaa tccccctttt ttctcatcat accttgattt
    11401 ccaaaaggag gataacgttt tgcttgtgac agaaaaaacg gaaactatta tatacgtgat
    11461 acggcttctg ataaatgcca aaacgaacgt acttgatcaa aaacggttaa atcaggaatc
    11521 acatattcag cagaaggaag atttatgttt tcacctattc ccaatacatg catgttacca
    11581 gccaaagctg cttgacaacc agcttctgaa tcttcaatga ccaagcatgc atttggcggt
    11641 acattcaatt gctgtgctcc tttgagaaaa acttctggat caggttttgc ttttgatact
    11701 tgcgtgccgt caataaggac atcaaaataa cttgtcaaat tcgttttttc taaaatgagg
    11761 cgtgcatttt tgcttgctga tcccaatccg atcttgatgt ttgttttttt caagtactca
    11821 agaacagcta ttgcaccagg caaaacggat gtttcatcca ttttttgaat cgcttgaaga
    11881 tatagattgt ttttttgctc agcaaaggct gatttctctt tttctgagaa tttctgttcc
    11941 atttttccaa atgacagcaa tcgctctagt gactcggttc tggatattcc tttcagttgc
    12001 tcgttttctt tctcagtgaa tggaatggac agctggttag ctaaaaccag ccacgcttcg
    12061 taatgataac gagctgtgtc tacaagaacg ccatctaaat cgaatagtgc tgctttgatt
    12121 ttcatcgatt acctccttga aaatgaacga cttgaagtaa agatgcgatg tttttagctg
    12181 tgcgatgaag attatcagtg gcttgctgta aagcttcttg taaaggcaca gctgcaggaa
    12241 ggatcggaaa aacggccgaa atgccaacat ctgctgcttt ttcgcttcct ttaccaacac
    12301 ttccgcaaat agctatcaca ggaacgccct ctggtactct tctagcaatc ccaactggcg
    12361 cttttccttt tagactttgt ccatctagac gtccttcgcc tacgattacg aggtctgctt
    12421 gctgtacccg tttatcgaac tccagtaaat ccaatacgta gtcgatgcca gagactattt
    12481 tccctgaagc aaatgctaac agtcctgcac ccattccacc accagcacta ctgcccgatg
    12541 cctcgattac ttgaggggca aatctcatat aaaaatcatg catggcttgg tcagccactg
    12601 ctaattcatt ttctttcaag cctttttgtg gtccgaaaac aaaagaagcg ccttcttttc
    12661 cgcataaaag attagtaaca tcagtaatga cagtgatttt ggctttaatc agctcaatag
    12721 gcacgttcgt tttatcaact gtagcaattt tacctagatt ttctcccagt gcttctactt
    12781 tttgattatc ttgatcgtaa aaagcataac ctagaccttc tgccatgccg atgccgccat
    12841 cgttggtact acttccgcca acgccgatga tgatgtgtcg aacaccttgt tgcaacaatt
    12901 gtcgtatcag ttctcctact cctcgcgtgg ataacctaag tggctgttgt tggtttagtg
    12961 ggacatcttt caatccgacg atttctgcca tttcaaaaat cgcagtttga ccgtctgaag
    13021 cgtactggat ttctttgttt ttgccaaatg cattagtaac gactgctgtt tgccgcttca
    13081 accttaaact gtctgctaat gatgaaaccg ttccttctcc tccatcgcct ataggtagca
    13141 gatcatattc agcctgaaca aaaacttctt gaaaacctga ttgaatggct tgtgctgcct
    13201 ctaaggctgt cagactttct ttgaaagaat ctggcgcaat cagaatacgc attattttcc
    13261 cctccgttcc aagatcagtt ctttttccag ctggtattgg tctccattga caaaaatagg
    13321 taatgtttct ttcgtctctt tttttattac tatttgtttt tttgtcaaaa caaatagcaa
    13381 cctgtgattt ttccaaataa aaggaaaaga aattttttcc catgctgaag gaagtcttgg
    13441 agaaatagaa agagttccat tcgttactgt aatacctgaa aaaccaaata taactgctag
    13501 ccatagtgct cctaatgaag cagcatgaat tccgtcatct gatgaatgcg gatctggtcc
    13561 taaatcaatc aggcacgctt cttggaataa atgatacgcc cattccattt cttggatacg
    13621 gcaagcctct atggcgtgaa tcgctttact caacgatgag tcgtggattg ttttgttttc
    13681 atagtaatgc aaattcttag aaacgattgg ttgagaaaaa tactcaggta ataagtataa
    13741 cagcatgaca agatctgctt gcttgctgac ttggagttca tttacttcgc tacgggcata
    13801 atcaagtaaa atagactggc tgccaggacg ttctttgtaa gtggtcaaat caataactgg
    13861 cttactgaga tacgtgtcat cttgcggaat cagctgttct ttgttaggca ctggcaaata
    13921 taacttatct aaaaaatttt gacattcttt caaaaaaagc ggatcgccag cttgatattt
    13981 aacagctaag gccacattat aatgcgccag atagttcgta tatgcattat tgtcgatata
    14041 ttctgtgtat tcatctggac caatgactct gtgtatctct aaatgatcat cgacttttac
    14101 agctctgctc agccaaaaag ctgctgtttc atgaatgagc gtttttccct ttgtttctaa
    14161 aaacgattga tcctctgttg cttggacata ctcttgaacg gcataagcaa ggtctgcact
    14221 gatatgatgt tctgcggcag ctgaagcaac ttcttgtctt gttccggttt tgatattcaa
    14281 tgcagcaaat ttgggtgtct cttcctgacc agtgtgtgcg ctttcccatg ggaaaagtgc
    14341 accttgatag tgataatctc ttgctttttg ccgtgcgcct tctaatcgat cataacggta
    14401 cagcagcaaa ttctttgcta cttcaggaaa ctgatataaa aagaaaggca ggataaaaat
    14461 ttccgtatcc cagaaaacat gccctttata accttcgcca gttagtccct tcgcccctac
    14521 actaacactt ggatcattgc ggggagtcat aatctgtaaa tggtaagcag cgaagtccat
    14581 cgctaactga tcgaaagaag aatcgccttg gatcgttatc cgatgctgat gccaaaaatc
    14641 tgtccaataa gacatcgact gctctaaaat ttctgtataa ctaacgcttt tctgggcttg
    14701 ggcaatactt ttcttaaacg ggtctgtttg ctgaagagag gtagagagca cgatgatctt
    14761 ctccagcgta aaggacgtat tttttctgat cttttctgaa aaggaagcgg tgatccggcg
    14821 attttttgct tggaatacac ctgctttatt gaatttagct gcaatcccaa tatcaatttt
    14881 actttctgtt gtctggtagg atgcactgat caaatcgtca ccaaatactc gcagttcttt
    14941 ttcaaccaac tgctgtgtac caaaattggt ttgctgtcca tcgatacccg ttttcacatc
    15001 tacttggcag tcttcggtca acggcttgat gaccactttc agggctgcct gatggagatt
    15061 attttgatca gcaacacgac aagtaatgac ttccactttt tttccttgag cagtttcaat
    15121 gagcgcatag cgtttaagct ctcccatttc taaatctagt gcccgctcgt atgcaagaac
    15181 tttcatcgtc tcaagagaaa aatgttcacc gtcgattttt atttcccatc tgatgacatc
    15241 tgggagattc actaactcgg ctgattctgc tcctaaaggt cggttatata caccagctgc
    15301 aaacattccc cttacttgat tccgatagtc ttcctcctgg cttcctctga taccaaaaaa
    15361 tccatttcca atggtcatct gactcgcata cttattgata tactctaaat caaactgttt
    15421 ttcttctact ttcataaggt aataacttct cctgtttctg ccgaacgata taaattttcc
    15481 accagattct ggatgacata tccttgccag ccatctgcaa tcatctgagt ctggtcaccg
    15541 agcgcccgat tcacaaaatg agccatactt agttcatgtg ggtcattaac tggcgtggtc
    15601 ttttgtaagg aaaccagctt tccatctaca tcttcgaata gttcgcctgg atacagatta
    15661 gcacctgcct gatcgccaca aaaagccaca ttcagtttgt cttctctgat attcaaagca
    15721 aacgaagtat tcagttggat gatcttgccg ttttctaatt ccagtgtgcc aaaaattgca
    15781 tcttctactg tgaatttgct tggatcccat ttcccgaatt tccctgaatt ttttttcgta
    15841 ccaatttttt ggaagctgta cgcccgaacg ctttttactt taggaaatcc agtaatatac
    15901 atcgctgtat caagcatgtg aatgccataa tcgatcaaag gcccgcctcc ttgaagttct
    15961 ttgtcgataa acgttcccca gcctggaaca ccagaccgac gaagcgcatt cgcttctacg
    16021 taataaatat ccccgagaac atcaagatga ttcttgatca ttgttgcttc gtcagaaaaa
    16081 cgatgctgga atccataagc caaaatctgc ccgtttttat cggctgtctc tgccatcttt
    16141 cttgcttgat cagctgtcat agctggtggt ttctcgcaca tcactgaagc attagcttcg
    16201 agggcagcca atacatgttc gaagtgaaag cgattgggtg tacagacact cacgatatcg
    16261 ggagaaatct cattcagcat ttgtgtaact gatgtatacg catgagaaaa tcctgtacgt
    16321 cggcaaaagt cttctgctcg ctggctgact ggatcaacca cagcggccaa ttctaagaca
    16381 tctgatcttt tttgataata agcagcatgt actttttctg ctacttgtcc agcgcctatg
    16441 attgctactt tcttcattta tttctccctc ctttgcttat ttacttattg ttattctttc
    16501 tctattcttt ctctaaatca tttaaatgac gcgctgtttc cagacagttt ttcaagtatt
    16561 gcaatgattc ctgataaatt ttcacttcat tttctcctcg caaacgacat tcatatacca
    16621 tataaccttc atactcattt tgaagtagcg tttgaaaatg gcgagtaaaa tcaattgcgc
    16681 cagatcctgg ttggtagcga tgattatctg ctaaatggat atgacccaca tactcttttt
    16741 gcgtagtcaa tgcttttgaa atatcatctt cttcaatatt catatgataa aaatcagcga
    16801 tgatctgcac atgtttcaat tgattagagt caatgtaatg acgcgcatcc gctaacaaat
    16861 tgatcatatg gtcttggtat cggttcaaag gctccaaata aataaccgta cctgtttctt
    16921 cggctacttt atctaaataa cgaagtgatt cgctgactgc tttatagtct ccttctttgc
    16981 ttcggggtga aaccatcggc ggtaatcgat aagtgaacat tccccaagct gctgggataa
    17041 cgattccttt gcctccaacc tcgcttagtg cacgcaaaat ctcagcaatt tgacgtacac
    17101 cattaagacg ccgttcttcg ataaagtcac cgatccaccc atcgtagccg ttacaagctg
    17161 tcactactgg gatccctgtt tctttgattg cctgtttgac tgtgtcgata ttttctatta
    17221 acaacttacc gtcgatttcg tattcttcaa atccacagtc tttgataaat ctaaattttt
    17281 ctaaaatgtc tcttgggaaa aatgcttgat cttgcgtacc gaatttcatt gttctcctcc
    17341 tcggacttga acgcccattt tgatactttc ttctgggtgc tggtctacat aacgcatgta
    17401 agcttctgcg ctttctgcaa atggcacaac tggatcaatg atttcttcac aatccaagta
    17461 accgttcatc aataattccc aacatgtgtc ctcgattcgt ttacggttcc atcttgggta
    17521 atctgggttt ggttcactag atgctcgagc aaagacgatt tttgcattat tgaaatgtgc
    17581 ttcgcgacca agattaaacc cagctggaaa aggtttagca aaggcaacat aagaaatgat
    17641 cccgccatag ccgatccctc ttaaagaagc ttgtaatgcg gcagcactgc cactcgtttc
    17701 aataactgaa tctactccac gtttatctgt caattccttt agacgaaaac cgacatctgc
    17761 tttcatagga tctagcgtgt aatcggcacc gtatttttct gctaattctc tacgctgcgc
    17821 tatgggatcg atcccaacga tggtagtagc acctgctttt ttagctaatt gaatcgcaat
    17881 ttgcccgata gcgcctaaac cgaagattgc tacagcatca cctgctcgaa catgtgcttc
    17941 ccggatacca cctaaagcga attgagcagg atcgtaacat actgcatttt tccattgatc
    18001 caaccgatca agtttacgta gtttataatt atcaacaccg tttgtaatca gcgtttcacg
    18061 aatgggtcca taagtgcaga ctaactctcc tttttgatac tctgtcacca aactgcccgt
    18121 ttcttcgatc gtaccgacaa tcatatttcc taattgaaat tcgccaaata ccacaccgcg
    18181 ttggcttcct tcttctcttg gcaagaatag gcgccattct tctgaaaatt cttcatcgat
    18241 aaatggactt aatcctctaa agtcgacaat ttctgttcct ggttttggag tagcgaaaat
    18301 tactcggata cgtacttcgt tcttttcgat ctcacggtct tcgtaatctt tgaatccggc
    18361 tattcttggt tctaatgcaa ctaatttttt catcttttct ttcctctttc ttttcttttt
    18421 atccttttac gccgccttcg actaaatcac ctttaataaa tcgttcagat aacgcataca
    18481 taatgatgac tggtaaagcg gtcaccaatg aagcggccat cattcgtccc caaatgtagt
    18541 ctggtgtact gaataatgtg ttcaaaccga ttggtaaagt aaatttttcg gaggtcgaca
    18601 ggaaaatcga agcaaataaa taatcattcc acgaaaccat aaagcaataa acgaagacag
    18661 aaatgattcc agaaatagcc aatggtataa tgatccgaat aatgatctgg atccggctaa
    18721 gaccatcgat cattgctgct tcttcaatat cagtcggaat cgtgtcaaaa taacttttta
    18781 gcatgaatat cgcagtagga agtgtttgaa cgaccatcgt gataatcagt gctgtattgg
    18841 tatcatacaa acctagaccg gaaatgattt taaataatgg aacaacgatc aaaataccgg
    18901 aaaacatata aacggtgtaa aagctagcat tgattttcga gcgagctttg aagcgtagtt
    18961 ttgacaaagc gtatgccccg ctgatcccaa tcactactgc agttacggag gctatcaagg
    19021 agatcactaa actatttttg aaatacatga tgaatggaaa aatctcagca ttgaaaatat
    19081 cctgataatg ttcaaaagtc cattctttcg gaaaaatagt ttgtgtcgtt gcaattgctt
    19141 cttgtgaatc cttgaatgat gtcatcagca tgatcaaaaa tggaaacaaa ttgacagcta
    19201 atagcaacaa aatcgtcgcg taaaataaga ttttcctgct ctttttattt ttcatttcaa
    19261 gtccaccttc tttcgtgaga gcaagacgac cgctgcaata atgatgaaca atagaatcga
    19321 tatagctgct gcttttccaa gatcattgaa cgcaaaggct gtctgataca aataaacgcc
    19381 taagatattg actttattcg tcagtagata gacatctgta aacatataga acatccagat
    19441 agatcgtaaa acaatcacag ttgctagtac tggtttgatt gccggcagtg tcacgatctt
    19501 gaacttctgc caagcatttg caccgtccat ttcagctgct tcataaagcg ttttgtcgac
    19561 tgtttgcaaa atagccaaaa atgaaataaa cgcatacgga tagtatctcc agatagcaaa
    19621 caaaacgacc ataaagaagc tagcaactgg ctggtcgaac cacagcggtg cttcgttgaa
    19681 aagctgcaac ctatctacta acagatcatt gacgattcca taactattat tgaacatata
    19741 tttccaagca aaaatcaaag aaatagatgg cgtaacataa cttaataaaa tcagtgaacg
    19801 caccgttttc ctaaatctga acttgcggtt aagcaaaata gctacggcta atcctaaagc
    19861 tgtactgcct gctacaacta agaatgtgta agcaactgtt aaacctaaag aacgataaaa
    19921 ttcactatca cttaaaattt caagataatt gcttaatccg ataaaaacgg cgtctgagtt
    19981 aggattcagc ggcatatcga aaaaactgat ctgaatattg gaaagcattg gaatcagcac
    20041 taaaacagct aataaaagca tgcttggtgc taaaagtccc atggctaatt ttaagtcaga
    20101 atgtttcctt ggacgattca ttgaaacctc cccctgataa tctgccaata gctatctact
    20161 tttttcgaac accattggca gaacttcttg ttatttcgta tttttttgtg cgtcagctaa
    20221 agcttgatcg acatcttcgt ttccgacagt tacttggttc actgcttttc cgatcacacc
    20281 tgagcttgtg atatctccca ttttcgtgaa attcttttcc ccgactagtc caaagacttg
    20341 tacttcatca aaagcactag caatctcaga tggcagctcg ccaaaagact tgatgacttc
    20401 attttctttg taaccatcca gttcgactac ttgtttattc actggttgag caccaccagg
    20461 agacatcaag acccactttt ccatgttttt cggttgagat aagaaagctg tgaatgcttc
    20521 agcagcctct ttttgtttct cgtctagacc agaagaaatc gtcaatccag atactgtgcc
    20581 atagacagct tgatcttttt gtgttgggat agcaaaaccg atattttctg agttccccgc
    20641 ttcataaaca gaaggcaaaa tgtaagttga atagattgcc attggtgctg ttccattcat
    20701 gaaagcatct ttgatttctg tcacatcatt agagcctggc atcgtgtatt gggataagtt
    20761 ttgataaaac gacaatgctt ctctcatctt ttccgtatcg atcgttacct cgccttcatc
    20821 atccaaaaca tttgctccat ttgacaaagc aaattgagag aacgcttgct cactcattgt
    20881 tccttcagca gtaggcatag caattccata ttgtttattc tctttatctg tgaaatgttg
    20941 agcaattttt aaaatatcat cccaattttc tggttctgaa aaaccagctt ctgataattt
    21001 ttctttgtca taccaaatac cttgaaccca gccgctaatt ggtgcagcaa tatagctttt
    21061 tccatcttct gaacgaacaa ggtttttggc tccgtcataa taattatctt ctccagcttc
    21121 ctcgataacg ttttggactg ccgtctgatc aatcagttca tctttatcca tcactttagc
    21181 aaaatcctga ctgacttcca ttacttctgg taatttaccg gaacgagcta acgtaacgat
    21241 ttttgtattg aacgctgatt cttctaccgg cacttgcttg attttgatgt ctggattttc
    21301 tttttcaaaa tcagcaacca gttcattgat cacacttaat cgttcctgtt cgacagaaga
    21361 gtgcatgaat tgaatcgttg tctgttgtga ttcgttagag ctttgacttt caccacaagc
    21421 agtaagaaga aatccagtag ctagcagaca ggtagtaaca ataatttttt tcattttttt
    21481 ccacctctta ttttattatt ttcagccaca tatattcata cggctggaat atcaactttc
    21541 gttcttgact gcaatggata atttgtccag aaaacaggtc taggtaatct ccttttggta
    21601 aaagataacc tgctgattga ttggaaaaat tatggataac taagactttc tcttccttcg
    21661 cttcgcgaat aaaactgata aaagaatctg ctgagtccag taagtaaatc aaactattcg
    21721 gatggaaggc tttttctttt tttcgaatag atagcatctg ggtcaattca ccatatactg
    21781 ctgttcttaa tggatctgtc tgcaactctt tctcaatatc tgctaacaaa aatttttgcc
    21841 gattgatttt tcgattctct cctgtttttt ctacgccttt gtaatcattt cgacttccca
    21901 gcacactttg aatataaatt gccggcacgc ctggtaacgt taccaaaaat gaatgagcag
    21961 ctaaaaagcg tttgcttctc cattccgtcg tatctgttgg agaacttaac gcatctagat
    22021 acgtagcgtt tatctcatat ggactggtcg ttccatctgg gtttttcttg tagtttactc
    22081 gggtaccctc tttcttcagc tgttccacta attcaaggat cgttttttcc ggcagaatac
    22141 ctcgtaccgg attcaatccg ataccgtcat gggaggctaa aaaattaaaa aaagttgttt
    22201 ttttcgatgc gaaatcgatg gtttttaacc attctttcaa cttacttgta ttacccgaac
    22261 gaatcgcttg aagggtcaaa ggtggtagcg aaaactgata gaccatctgt gcttcattct
    22321 cgccattccc aaaataactg atattatctt tgtgtggcac atttgtctca gtgatcaata
    22381 ccgttcctgg agctgcttca tcagcgattg cacggaataa ttggatgatt ttatgcgttt
    22441 ctggcaggtg tatacaagaa gttcctagtt ctttccataa aaatcccaca gcgtccaagc
    22501 gaataaatga agcgcctttt tctaagtaaa acaaaagaat atcgatcatt ttcaataaaa
    22561 cttctggatt tttaaaattc aagtctactt gatcttcgct aaaagtcgtc catacgtgtt
    22621 tttctttccc gtttttgtca atgaacgttg taagtaacgg gctggtcctt ggtctagtca
    22681 cgctgcttgt atctgtatcc agtggcaaat caataaaaaa atctttgtac gtatcattca
    22741 agtttaaata ttcctgaaac caagtacttt cggcagacat atgattgcag acaaagtcga
    22801 acattaaccg tgtttcgctt ctcatttctt ctatatcttc ccatgtgccc aaacggtcgt
    22861 tgattgcctg ataatttaca acggagaaac catcatcaga tgtatatggg aaaaatggca
    22921 agaaatgtac aactctaaat gtatctgcca aatacttttg ataaaatttc ttaaatgtac
    22981 gcaaagttgg ttgctgctct tcaaaaaatt gatccccata agtaatcaag aaaatatctt
    23041 cttcgtccca atgcagtttg cctgcctggc ctttcttctg ctgataaaca ctgacttttt
    23101 gttcaatcat agaggcggtt ttctcagaca aatctgagtc gcgataaatc gcttgtaaaa
    23161 gttttgtata catcggattc ccctttcttt tttgggagcg ttcccaacga tttgatcata
    23221 ttatggaaaa cgcttactgt caatagcatt tttttaaata attttgatat acacaaaaat
    23281 acaaataaga aagcttatgt atcaataatt aatagcttct taaaaaaata tggtctctca
    23341 tgatcatgca agctattcct aaattttcaa aatcatagac aaaaaagaat gtctattgta
    23401 ctatggaatt attctaaaac gctcccaaaa aggaggtgca tataaaattg aaacctacta
    23461 tttatgacat tgcgaaaaaa gctaatgtct caaaatcaac tgtctccaga gtattaaata
    23521 accagccaaa tatctctgat aaagcaaaag caagtgtact tcaagcaatc aaagaattga
    23581 attatgaacc gagcaaaatt gcaagaggac tgtcaggaaa tggttttgac gcgattctag
    23641 cattatctca tcgtacttct catacgacct atggcaatcc ttttttttca gatgttattc
    23701 aatccatttc tgccgtttct gaacaaaata acttcgactt gattctacaa acggcaaaaa
    23761 caccagaaga agagctagaa aaatgcgcga aaaagatcca agaaaaaatg atccgaggaa
    23821 ttattatttt aagctctacg atggatgaaa cacttctttt tgaattggat caatacaata
    23881 ttcctattgt ggtaatcgga aaagtcggaa acgactacac gaacatctac tcagtggaca
    23941 cggataattt ccaggatagt tacgatttgg tggaaacact gatcaagaat ggacaccgtc
    24001 atatcggctg tctccatgct ccgacagaag tttatgtatc caatgatcgt ctcaatggtt
    24061 accgtcaatg cttatttgat caccacctag atatccgtaa tgaatggatt atagacggcg
    24121 gctttcgctt agaagatagc atggcagcaa tgaatgattt aatgaagcta cgagaaagac
    24181 caactgcgat tttttctacc gatgcattaa aatcattgag tatttacaaa gtggcagaac
    24241 aatctggaaa gcagatacct gatgatcttt cgatcgcagg attcagtgat cccttattgt
    24301 cctccctatt ttcgccacag ttgactcgga tagacattcc gacaaatcag ttaggaatca
    24361 aagccacaga acttttattc caactaatcc accaccaata ccctgaagaa aaaagaatca
    24421 ttctttcaac cgagattact ctcacagatt cgatccaaca acaagaaatg tagaacagaa
    24481 ttataaaaga acagaaacac tgaggataag tcagttactc tgttcttttt tcaatcactc
    24541 atccctcgtt accccctccc ggtattttag cgaatttttc ttattttcca tctgttttag
    24601 atagctgtgg ataactataa tgtttgtgtt aaactatttt ttccacaggg aagaaaaaac
    24661 tatttttaac cacgttgtgt ataagttttt atagttcgtt tttttaaaca catttaccct
    24721 tatttcaatc catg
//
LOCUS       DAYYCW010000033        24426 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000033 DAYYCW010000000
VERSION     DAYYCW010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.033"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACHAYA_001233"
     CDS             complement(53..787)
                     /locus_tag="ACHAYA_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8605041.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="ACHAYA_001234"
     CDS             1114..1524
                     /locus_tag="ACHAYA_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM8605042.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="ACHAYA_001235"
     CDS             1517..2218
                     /locus_tag="ACHAYA_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM8605043.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="ACHAYA_001236"
     CDS             2218..3804
                     /locus_tag="ACHAYA_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8605044.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="ACHAYA_001237"
     CDS             3823..4044
                     /locus_tag="ACHAYA_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM8605045.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="ACHAYA_001238"
     CDS             4041..4802
                     /locus_tag="ACHAYA_001238"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM8605046.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="ACHAYA_001239"
     CDS             5182..5691
                     /locus_tag="ACHAYA_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8605047.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="ACHAYA_001240"
     CDS             complement(5766..6305)
                     /locus_tag="ACHAYA_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM8605048.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHAYA_001241"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHAYA_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM8605049.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="ACHAYA_001242"
     CDS             7318..8169
                     /locus_tag="ACHAYA_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM8605050.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="ACHAYA_001243"
     CDS             8197..8832
                     /locus_tag="ACHAYA_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFM8605051.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="ACHAYA_001244"
     CDS             8852..9625
                     /locus_tag="ACHAYA_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8605052.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="ACHAYA_001245"
     CDS             10033..10830
                     /locus_tag="ACHAYA_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM8605053.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="ACHAYA_001246"
     CDS             10808..11221
                     /locus_tag="ACHAYA_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8605054.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="ACHAYA_001247"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="ACHAYA_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8605055.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="ACHAYA_001248"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="ACHAYA_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM8605056.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="ACHAYA_001249"
     CDS             15998..16558
                     /locus_tag="ACHAYA_001249"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM8605057.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHAYA_001250"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHAYA_001250"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM8605058.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="ACHAYA_001251"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="ACHAYA_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM8605059.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="ACHAYA_001252"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="ACHAYA_001252"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM8605060.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="ACHAYA_001253"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="ACHAYA_001253"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM8605061.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="ACHAYA_001254"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="ACHAYA_001254"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM8605062.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="ACHAYA_001255"
     CDS             21905..22483
                     /locus_tag="ACHAYA_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8605063.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="ACHAYA_001256"
     CDS             22535..23017
                     /locus_tag="ACHAYA_001256"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM8605064.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="ACHAYA_001257"
     CDS             23134..23508
                     /locus_tag="ACHAYA_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605065.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            23938..>24426
                     /locus_tag="ACHAYA_001258"
     CDS             23938..>24426
                     /locus_tag="ACHAYA_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFM8605066.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMD"
BASE COUNT         8007 a         4390 c         5213 g         6816 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggtat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttttt gagagtataa aatattttgt gtaaatgaaa aaatccatac aaaaaaggaa
    23881 gtcccttctg tagaataaag ttaacgacaa ccaattcaca gaaaagagga cttccctatg
    23941 aatgatttta ctacagaaat tgtgcaaact ctagtcacta aaggcgattt aaatgaatta
    24001 ttccgttcgc acttagaaaa agcgataaac acactcctac ggactgaatt aacggctttt
    24061 ttagattacg aaaaatatga tcgcactggt tttaattcag gtaattcgag aaacggttct
    24121 tactttcgat caatcaaaac cgaatatggt gaattaacat tggaaatacc tagagatcgt
    24181 aatggtgagt ttaaacaaca aactttacca gcctacaaaa gaacaaacga tacattggaa
    24241 accactatta tccatttatt cgaaaaaggt gttacgatgt ctgaaattgc tgatttgatc
    24301 gaaaaaatgt acggtcatca ctatactcca caaaccatgt ccaacatgac taaagttctg
    24361 actgaagaag taaatgcctt taaatccaga gccttaaatg ataagtatgt cgctattttt
    24421 atggac
//
LOCUS       DAYYCW010000034        24315 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000034 DAYYCW010000000
VERSION     DAYYCW010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.034"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..885)
                     /locus_tag="ACHAYA_001259"
     CDS             complement(37..885)
                     /locus_tag="ACHAYA_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8605067.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1109..3049)
                     /gene="thrS"
                     /locus_tag="ACHAYA_001260"
     CDS             complement(1109..3049)
                     /gene="thrS"
                     /locus_tag="ACHAYA_001260"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM8605068.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3386..3565)
                     /locus_tag="ACHAYA_001261"
     CDS             complement(3386..3565)
                     /locus_tag="ACHAYA_001261"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM8605069.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3612..5435)
                     /locus_tag="ACHAYA_001262"
     CDS             complement(3612..5435)
                     /locus_tag="ACHAYA_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8605070.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5713..7992
                     /gene="helD"
                     /locus_tag="ACHAYA_001263"
     CDS             5713..7992
                     /gene="helD"
                     /locus_tag="ACHAYA_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM8605071.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8085..8732)
                     /locus_tag="ACHAYA_001264"
     CDS             complement(8085..8732)
                     /locus_tag="ACHAYA_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605072.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8911..9630)
                     /locus_tag="ACHAYA_001265"
     CDS             complement(8911..9630)
                     /locus_tag="ACHAYA_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8605073.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9761..10912)
                     /locus_tag="ACHAYA_001266"
     CDS             complement(9761..10912)
                     /locus_tag="ACHAYA_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM8605074.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11129..11869)
                     /locus_tag="ACHAYA_001267"
     CDS             complement(11129..11869)
                     /locus_tag="ACHAYA_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8605075.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11873..12196)
                     /gene="rsfS"
                     /locus_tag="ACHAYA_001268"
     CDS             complement(11873..12196)
                     /gene="rsfS"
                     /locus_tag="ACHAYA_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM8605076.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12253..12846)
                     /gene="yqeK"
                     /locus_tag="ACHAYA_001269"
     CDS             complement(12253..12846)
                     /gene="yqeK"
                     /locus_tag="ACHAYA_001269"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM8605077.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12830..13480)
                     /locus_tag="ACHAYA_001270"
     CDS             complement(12830..13480)
                     /locus_tag="ACHAYA_001270"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM8605078.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13490..13801)
                     /gene="yhbY"
                     /locus_tag="ACHAYA_001271"
     CDS             complement(13490..13801)
                     /gene="yhbY"
                     /locus_tag="ACHAYA_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM8605079.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13817..14926)
                     /gene="yqeH"
                     /locus_tag="ACHAYA_001272"
     CDS             complement(13817..14926)
                     /gene="yqeH"
                     /locus_tag="ACHAYA_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM8605080.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14923..15450)
                     /locus_tag="ACHAYA_001273"
     CDS             complement(14923..15450)
                     /locus_tag="ACHAYA_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM8605081.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15630..16415)
                     /locus_tag="ACHAYA_001274"
     CDS             complement(15630..16415)
                     /locus_tag="ACHAYA_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM8605082.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16408..17277)
                     /gene="accD"
                     /locus_tag="ACHAYA_001275"
     CDS             complement(16408..17277)
                     /gene="accD"
                     /locus_tag="ACHAYA_001275"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM8605083.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17278..18657)
                     /locus_tag="ACHAYA_001276"
     CDS             complement(17278..18657)
                     /locus_tag="ACHAYA_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM8605084.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18665..19084)
                     /gene="fabZ"
                     /locus_tag="ACHAYA_001277"
     CDS             complement(18665..19084)
                     /gene="fabZ"
                     /locus_tag="ACHAYA_001277"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8605085.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19109..19585)
                     /gene="accB"
                     /locus_tag="ACHAYA_001278"
     CDS             complement(19109..19585)
                     /gene="accB"
                     /locus_tag="ACHAYA_001278"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM8605086.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19588..20823)
                     /gene="fabF"
                     /locus_tag="ACHAYA_001279"
     CDS             complement(19588..20823)
                     /gene="fabF"
                     /locus_tag="ACHAYA_001279"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM8605087.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20842..21579)
                     /gene="fabG"
                     /locus_tag="ACHAYA_001280"
     CDS             complement(20842..21579)
                     /gene="fabG"
                     /locus_tag="ACHAYA_001280"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM8605088.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21591..22508)
                     /gene="fabD"
                     /locus_tag="ACHAYA_001281"
     CDS             complement(21591..22508)
                     /gene="fabD"
                     /locus_tag="ACHAYA_001281"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8605089.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22615..22839)
                     /locus_tag="ACHAYA_001282"
     CDS             complement(22615..22839)
                     /locus_tag="ACHAYA_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8605090.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22892..23854)
                     /locus_tag="ACHAYA_001283"
     CDS             complement(22892..23854)
                     /locus_tag="ACHAYA_001283"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM8605091.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23864..24304)
                     /locus_tag="ACHAYA_001284"
     CDS             complement(23864..24304)
                     /locus_tag="ACHAYA_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8605092.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6651 a         5419 c         4241 g         8004 t
ORIGIN      
        1 tattctaaaa aacttatata aaaaatagta aggtttttat ttcgaatcca taaaatattc
       61 ttggattgct tctcgtgttt ttttcacttt ttcgctaagc ttttcgtttt tagccatata
      121 gaaataaaaa atgatatcga tcgtagcaaa ttgggccaaa agtgaattag ttgccgcact
      181 tcgattcgtg atttcttttg gtctagatgt ttgtacgagt acatcggcta tcttgctaag
      241 cgggttattt ccaaattgcg tcaacgcaat gatttcgata ttcatgcttt tcgccagctc
      301 ggctaatgcg actacatctg cacttctacc tgagtttgaa acaagccaaa gcactttctt
      361 tttctcattg ctgacaagtt gcgggagcaa gacgtggata tctttttcaa aaatgattgg
      421 cttaccgaca cgagaccatt tttgcagaat atcttctact actaaagaag aggcgccaac
      481 cccatataaa aaaacagtct ctgccgattc caaacactct acgacttttt ggatcgtttc
      541 ttcttctaga atatctactg tttcttttat cgctacttgt gcattgttac caagtttgtt
      601 tttgatagag taaaaggact catttgcttc tatatcacta aaaccttcat cgattccagt
      661 ctgtagttct gttgaaatcg tgattttgaa gtccgtcaga ctttgaaaac cgattttttt
      721 ggaaaaacgt acaactgtcg gcgcactcaa cccggaagct tctgctaatt cattagctgt
      781 cattgtaggg accttctcaa tattaccgat aatgtagttg gccaactttt tttctgctga
      841 agaataatct tccatcatac tttcaatacg actaattacg ctcataagtt cctccaccct
      901 tttaatgata gtctatgtaa gtataccata tcagaccctt attcacggtc gtatactgga
      961 taaaaaacaa aaaaacaatc cgcccaacag aatatcttat cccatcaatc ccacagattc
     1021 tattgagcat ctgtgtcttt gaggaatacc tgttatctat tgttcggatt gctttctatc
     1081 tactactttt ttacaggaaa tttacctatt agttttctct gctgtaattt ttgacttctg
     1141 cttccatagc atcaataaag atattcaaat cttctacagc tgtttcttta cttccataac
     1201 gacgtacgtt cactgttgca tcttccaatt ccttgtctcc aacaactaat tggtaaggga
     1261 tcttttgtgt ttgagaggca cgtatcttat agcccatttt ttcattacga tcatcaactt
     1321 ctacacgcaa ccccttcatt tgcaaacgtt ctttgatttc ataagcataa tcgccatgtg
     1381 catcaacaga aacagggatg attgttgctt gtataggtgc caaccatgtt ggaaaagcgc
     1441 ctttgtatac ttctgttaaa taagcgacaa aacgttccat tgtagaaaca attccgcggt
     1501 ggataactac tggacgatgc gtattttccc cgtcttctcc aacatatgtc aaatcaaagc
     1561 gctctggcaa taagaagtct aattgtatag ttgataatgt ttcttctgta ccaagagctg
     1621 ttttgacttg aacatctagt tttggtccgt aaaaggctgc ctcgccttct gcttcaaagt
     1681 aatctagttc taattcatcc atagcggctt tcagcattgc ttgggctttt tcccacattg
     1741 cgtcatcatc aaaatattta tctgtattgt ttggatcgcg atagctcaag cggaaacgat
     1801 aatctgtaat attgaagtca gcatatactg ccaccatcaa ttcaagcgta cgtttaaatt
     1861 catctttgat ttgatcagga cgaacaaatg tatgaccatc attcaacgtc atttctcgta
     1921 cacgttgtaa acctgacaaa gcacctgatt tttcatagcg atgcatcatt cctaattcag
     1981 cgatacgaat tggtaattca cgataagaat ggatcgtgtt tttgtagacc atcatgtgat
     2041 gagggcagtt cattgggcga agaacaagca tttcgccatc tcccatatcc attggcggga
     2101 acatgtcttc ttgataatga tcccagtgtc cagatgtttt gtacaactca acatctccca
     2161 ttattggtgt atatacatgt tggtaaccta gactaacttc tttgtctacg atataacgtt
     2221 cgatcgtgcg tcggattgtt gccccttttg gaagccagaa tggcaaacca gaacctactt
     2281 ctggggaaac catgaacaag tcaagttctt ttcctagttt acgatggtct ctttcttttg
     2341 cttcttcacg gagacggata tattctttca aagctttctt gtcaaaaaag gctgtcccgt
     2401 atacacgttg catcatttga ttgtttgagt ttccacgcca ataagcacct gccactgata
     2461 ataatttaaa tacttgtata cggccagtgg atggaacatg tggtccacga cacaaatcga
     2521 tgaattcgcc ttgttgataa gcagtaatca cttcttcttc tggcaattca ctaatcagtt
     2581 ctactttgta cggatcactt gcaaaaattt ctaatgcttc ctgttttgaa agaacacggc
     2641 gttcgatcgg cagattttct ttgacgattt tcatcatttc tgcttcgatt gccggcaaat
     2701 cttctgcagt tacttggttt tgtccattat ctgtatcgta gtaaaaccct gaatcgatag
     2761 ctggtccaac gccaaagtgg atatttggat agaggcgacg catggcttgg gccatcaaat
     2821 gagctgctga atgacggact aagccaagtg catcttcatg gtctggtgtg atgatctcaa
     2881 tacttgcatc ttcctcgatt ggacgagtca aatcaacgag ttttccgttc actttaccag
     2941 ctaaagcttt ttttgctaag ctttttgaaa tgctttcagc aattgccaat gttgttgttc
     3001 cagactcaaa ttctttgact gcaccatctg gaaaagtaat ttttatcata atagtttcct
     3061 ccttgaaatg ttttgattga tcagatcatc aagctgaaaa acaaaaaaat cctagcatct
     3121 cttgaaaaga gatactagga cgaaaagttc gtggttccac ctaatgttca aacgcaaatg
     3181 cgttcctcaa gcgttgtaac ggtccgaccg cccttgcttc aacaagggtt tcgaaagtgg
     3241 tcatcctttg gttttctcct ggaaaagttt cagcctagct tttcctctct tatcgaagac
     3301 tctcaaattc agttggcttt ctcatcaatc aattatgtgt ttatttaacc actaataaat
     3361 agaaaaatca agtgggaaac taaacttatt ttttccattc cccatttaca ccgtatgttc
     3421 cttttttcat ctcattgatg atgacatgga tgtgttcttt tggtgcctgc gtgtttttat
     3481 gaacagcttc cgtgatatct ttcatcatat tttctaattg ttccggactg cgaccttcta
     3541 ctaattcgac atgtacgaat ggcataatgc tctctcctct ttctgatcgt tcttttttca
     3601 actcgattct atcatactat gtttgtcttg ggtactgaat caggaaaaat ggctggatct
     3661 ttttttgctc tcaaggcact caaaatagag accgtatcca cgacttcttg caaaacagct
     3721 ccgaataatg cggggattag tcccgtactt gcaattaaca tcaaaaaaat acaaataaag
     3781 atgccaatca gtacagactg tttcgctatt ctcatcgtat ctcttgaaat cactactgct
     3841 tttgctacac gcaagagatc atctttcaag atgaccgcat ctgcactttc actagcagct
     3901 gtagaaccat gagcccccat tgcaataccg atatctgcag cagcaagtgc tggtgcgtca
     3961 ttcacgccgt ctcctaccat cgcaacaggt ctttgatctt tagggatact ttctaaaatg
     4021 gtcagtttat cttgaggcaa gctacgggca tgtacagaat ctaattggac tttttctgca
     4081 atcgtttgag caatggcaga tccatccccg gtgatcatca tcaaattttt cacatcaagt
     4141 tttcttagct ccttgattgt ctttgctgct tctgggcgaa tctgatccga aaataggatc
     4201 gttcctgcaa aagatcgatc aattgttaca tagattgctg tattggacgt ttctatttct
     4261 tcagctgaaa caaaagaggc acgtccaatc tttacttcat gattattgac tactgcttgg
     4321 attccttgac cggtcacttc tttcagttga ctaacaggat acagttggct gtttttcacg
     4381 ttgttcacta acgaacgagc taagatatgg ctagattcct gttctgcact agctgcatac
     4441 agaagtagct cttctttttt tattccattg actggctgaa tcgcctctac ttccaatatc
     4501 cctttggtaa gcgttcctgt tttatcaaaa gcaatcgttt ttgtacgtgc gagcttttca
     4561 atcgttgttc cagtttttac aacgatcccg tttttacttg aacggctcat tcctgcaact
     4621 aaagcaatgg gcgcagctag aatcagtgga caaggtgatg ccactaccag tacctcagca
     4681 aatcgtaaag ggtcttttga gaaccaccaa gaaataccac cgatcaaata agcaatcaac
     4741 gtgaaaggga ctgcataacg atcagctaaa cggacaaatc gggcaggctt ttcttttgat
     4801 tcttcgacta gtttaataat catttgatac tggctgtctg cggcacgttt ttcagcgatc
     4861 atttggatcg atccgtctcc atttaaagac cctgacataa tctgatctcc aggtttcttg
     4921 gtttccgggc gggactcacc tgtcaaagaa gattcatcga ccgaagattc tcctttggtc
     4981 actcgaccat ctgcgggtac aagttcgcca ggcttgacta gtaactggtc atcaatattt
     5041 atttcatcga ttactatatc ctgcaatttt ccttctttga atcgatgggc aattcttggg
     5101 gtgttatcta acaaagactg cagttcttta cccgctcgtt tattcgcgta atcctctaga
     5161 ctatctccgc ccgtaagcat gatcaatatc atcaaactag cccagtattc tttgacggct
     5221 aatgttgcaa ggattgcggt aatcgctaat acatctacgc catatcttcc tgaacgtaac
     5281 gtcttgatca tttccaaaaa catcaagaag gcgagaatcc ctccagtaaa tgcgacaatc
     5341 gcaaatgcaa gggccggctg gtgaaaaaca aattcactta ataaagctat cccaccaata
     5401 agcaaagtaa gtaaaaattt tttgaaatgt gtcattgttt tttctcctct tttatgtgac
     5461 ttagagaggt tagtaataaa tttccagctg cctctttcat cttaagcata gtccttgact
     5521 agcacttttt aaaacaatag gccttaattg aaaacaacta tcactatcag cgtttccaaa
     5581 attcatcagt tctacaaaca aaaatattca gcaaggatta ctttttcgat tcatcagtta
     5641 aaaagtacct tttttcgaaa aactatgcta tacttttaat tcgttattta cggcggaagg
     5701 agcaaacatg cgatgaatga gaaacaacta gaacaaacgc acgtggatga aacgatcgaa
     5761 ctaatccaga tcgaacaaaa tatgctggat acaaaacaac aagaattgac tcaggaaatg
     5821 aatcattcga caaaagacac agctaatcac aagattcgag ctggttccaa tgaagctttt
     5881 tatgaatctg ctgttgaata tcgccaacat gagcaggaat tgcttttgaa ataccatact
     5941 cttgaagcac agcaaaaacg cctgcaaacg ttgtctgtca tgaaagatag tccttatttt
     6001 gcccgtatcg actttaaaga agaccgtgaa acagaaacat tatatctagg tatcgcttct
     6061 ttaagagatc tggcagaaga aacaatcgtt attgactggc gcgcaccaat tgccaatctt
     6121 tattatgaag gtgagattgg tcccgcattt tatgaaactg aaacagatag aatagatgtc
     6181 gatctgcagt tgaaacgaca atttaaaatc caagaaggac gtattttatc tatggtagat
     6241 acgtcggaag taatcaatga tgactttttg ctagagatat tggatgaatc atcaagcaac
     6301 catatgaaaa acatcgtctc tacgatacaa aaagcacaaa atgcaatcat tcgggacaca
     6361 acaagtaaag taatgctgat tgaaggaatc gccggaagtg gaaagacatc cgctttgctt
     6421 caacgtattg cttttctgtt gtatcataat cgcaagtggc tcgaggctga aaacgttctt
     6481 ctattttcac ccaaccattt gttttctgat tacatctcga ctgtattgcc ttcattaggt
     6541 gaaagcggcg tgcctacaca gacattcaaa aattatttgg tgcagctgct gccagagttt
     6601 tctttactag atgaacagca acaagaaagt ggttttttgc taggggaaaa agatccgata
     6661 caagtgatga aaagcggatt gaccttagtc gatcagattg atcgctatat ccaaggcatc
     6721 acaaactacg gtcccttgtt ccgtgatatg aaaataaatg gccgtacgat cctatcaaag
     6781 gaatcgattc gccaatggta taaagaaaca aatgaacaat tacctttaca tcatagactt
     6841 tctctattac aaacgaagct cctgaaaaaa ttaggcggtc tgcaaaaaga cgaaacacgt
     6901 cagcaatggg tcaaagatct ggcggaagaa cagctccaag aactatacgc aacagatcca
     6961 aatcttgaat atacagaaca aaaggagcgt tcattacgta aaaaactggc caaccaaatc
     7021 gtacgtaaaa gattccgtcg aatccatcga gggatcatga attatcaatt tgtcaatata
     7081 acgaaacaat atctccactt tctacaaact gtgccacttt ctcttctaac acgacataaa
     7141 atcagcgaga ccaggtggca ggaacatttg acaactgttc gtacacactt gcgtcaaaga
     7201 gaattgcgtc aagaggatgc tgtactattc tttttactga tgcgccgaac atatccagtg
     7261 acggttacac acaaagctcg ctatatcttt attgatgaaa tgcaggattt cgcaccatca
     7321 caagtggcat tgctgcaagc cttgttccca aaagcaaatt tgactttctg tggagatcta
     7381 aatcaaaatg tctttggaaa tgaaacgatc gttggatcat tggaagacct tttcgtggac
     7441 cgtgaagtca ctcgtttcca attaacgaca agctatcgct ccacaaaaga aatcactgat
     7501 tttgctaatc attttattgt gaaagaacat caagtagaaa caacagcaag aaaaggagac
     7561 cttcctttgc tcatccaagg aacaacagat gatatcaaat tgaattggtt agcagatacc
     7621 attcgaacga ctcagggaaa agcccggtat tggcgtacag caatcatagc taaaacacaa
     7681 gaagaatgcg aagctcttta tgctcaactg ccagacgagt tgaaacaaca agtccagctg
     7741 attttaaatg agagtgactt tatgaagcgt tccattattt tacttccagc ctttttagca
     7801 aaaggtctgg agtttgaccg agttttcttg tgggatgtag atgcagaaca tttttcttct
     7861 cagcaagaca aattgatcct ttacaccatg tgtacacgtg cgatgcatga attgaccctt
     7921 tttacctcca ataatgaaag tcctttgtta aacaatatgg atatttcatc ttatgagaaa
     7981 aaagtactgt aagtaaaaag acaactgcta tcattaatgt tagcagttgt ctttttattt
     8041 acaataataa ttgcagaaaa agtaattcaa tcatttaagg tcatttattc tatatataaa
     8101 tccaaatctg tttcaggata ttttaatttg acaagcgaaa taatcatttt cagttcttcc
     8161 atgttttctg gatattctgg gtcaaatacc ttgctatgac ggccaatccc gccattgtcg
     8221 atcaatcggt aaattcgatt ccattcaatg aattggtcaa ttgattggaa attgctgatt
     8281 ggatagccgc cacaatccaa aatctctgat acacgaaatg ctaggtatgc actatcgatc
     8341 actttatttt ttttcgtgtc tatcttctca tttctttcga taagaatttt ttcaggacga
     8401 tgtttcaagc tccactctaa tagtagaaag aaagcctcca tccgatactc ttcttcaaat
     8461 aaccgaaagg ctgaatcaga acgacgaaaa agcagcggaa tcagataatt ttgtctataa
     8521 atcagcattg gtgcgcggtc caccatacac tctaaataac ggttcagata ccctgtaccg
     8581 acatagtttc ctaatcgata gccttcaacg ccgaatcttg cgtatttttg cgctaatttt
     8641 gaacaatcag ttacgttcac agctaactct tctactttgc ggtaaccgct taagatacgt
     8701 tcaaaaggtt ctaaagtgat ttttgcttcc atattcttcc ccgtccatct tccacatact
     8761 tcatatcatt tcacatgata cttatgctct catcatacca caggttgaat tagaaaagaa
     8821 aataaatcac acaaaaaagc aagacaatct gttagtgaga ttgtcttgct tttcgaaaaa
     8881 gactgaatac ttttcgttaa aactggttgc ttagtcttct aatgccacat tatggaacac
     8941 atgctgtaca tcttctagat cttccagtgc atcaatcatt ttttcaaatg tttcttgatc
     9001 ttctggagat agtgttacct cgttttgagg gatcatttgg atctcagcga ttgaaaaatc
     9061 agtgattcct ttttctttta atgcttcttg tatactatgg aaatccgcta cttcaccgta
     9121 aacgatgatt tgtccttctt cttccattac atcgcgaaca tcgatgtctt tttccattag
     9181 gtattcaagg atctcgtcag cgtcttctcc tgcaaaagca aagattgctg tattgtcaaa
     9241 catgtaagaa accgctccgg aaacacccat gttcccgcca tttttcccaa atgctgcccg
     9301 tacgtctgct gctgttcgat tcacattgtt cgtcaatgta tcaacaatca ccattgagcc
     9361 gcttggtcca aatccttcat agcgcaattc agagtatgtt tcatctcctg caccttttgc
     9421 tttttcgatt gctcggtcaa taatatgttt tggtacatta taagttttag cgcgatcaat
     9481 gacgaaacgt agtttttggt ttgcatgcgg atcaggatca cctgattttg ctgccgcgta
     9541 gatttcaatc ccaaattttg cataaatccg gctattatta gcatctttag cagttttctt
     9601 tgctacgata tttgcccatt tacgtcccat attttcactt cgctttctga tttgatagtt
     9661 tcataacgca aaaacgtcct gttccattat acttgtgaat cacgattctg tgaagctttt
     9721 tttctttctt ccttcattct tctcattttt agtcaattat ctatactcgg attgggattt
     9781 gcccgaaatt ctgttccgga atattgcctc ccaattgata gatttgatca cttctaacta
     9841 acagttccgc ttcttgtgcc tctttttttc cgattcggga ataaatagaa atctcttttt
     9901 tttcttttaa gaatcttctt ccagcatcgg taaatccaag tagtcgaaga taatttttat
     9961 tttgttggac aggaatctct tgctttttga tattcagcaa gacataacaa gataatcgtt
    10021 ggatccgtgt ccatgtatat ctttttgttt ttacctttga cacaaaatcc tcaaaactat
    10081 cagcagttgc agctgctgtt ttcatgcgcg attctattcc ttcggtcatc tgatagatat
    10141 ctgtcagttc cagcaaagta gaagataaca accgataacg caaatacggc caataagatt
    10201 cccaagtctg cagcgaacaa gttgaaagat cttcttctgt ctgaggcggc acaagtccag
    10261 ctattctttt tccttcttct aacgcatggc gaatggctgt tgcactggct atcctctgat
    10321 ctgtgatgat tttttcatga tagcctgcac ttttacgtcg gatcggatgt aattccatag
    10381 ggtggacata cttggcattt tcctttgcat aacttaatcc caagatatga tttggcgaag
    10441 aaaaatcaag cgtaatatcg ggatacagac tgcgaaaaac ctcagtcatt ttttgcggat
    10501 aactatacgt ttgatcagac agttcttgaa atgcctcttc tatcctatct tgattctctt
    10561 ggacaaagcg tccgaaactg ctgtaatcaa acgaagtctc ttcttctgtt ccaaaacaca
    10621 aactgtcaca gtccagtgtt tgcaagattt ttactgcacc tttagcaaaa tagtcagctg
    10681 actgaaccgc ccattcgacg ggaagctcca ctactaaatc cacaccattt tgtaaagctt
    10741 cattcgcgcg atgccattta tcgatgatag ccggttcgcc tctttgcaaa aaattcccgc
    10801 tcattacagc aatgacgacc tctgctcctg ttatttttcg tgccatttcc acatgatagc
    10861 gatgtccatt gtgaaaagga ttgtactcta cgataatccc gcagcttttc atgcgcatcc
    10921 tccttcatag ttatcgataa aaaagaaata gagacaagta aaaaaaggca aaaatcggaa
    10981 aaaatacccg aactatataa actattctcg caaacacaat acttctgcga cgaaaagctg
    11041 cactgcaaga gcaatgccta tatcgttcgg atatataatt tatgttgaag atttattctt
    11101 ttcttgttaa ctcgagcagg caaaaccgct agtctttgta tgctacaaag aaccagcgct
    11161 ttgatttctc tgatgctgct tcgtctgtaa aatcagaaaa tgcttcgact ttcgaaaagc
    11221 cgctgttttc cagcattctt aaataacttt ccaatggata ggtacgctct tgatgtagtt
    11281 catcttcacg aataaatcga ccagaccctt cttgttcttc aacgaaaaag gtaagaaaat
    11341 gttcgatgct gtgtttctcg cttcctggat aactatccca caaaaaagca aaattatccc
    11401 cttgataatg atagctatat tctggaaaaa cctcatctac ttgataggtg gagtgtacgt
    11461 caaataaaaa tcggccattt tcttctaacg cttgatagac ttcatcaaac acttgctgta
    11521 cctcttgtct atttttcata tagcacagcg aatcagaaaa acaggtaatt gcctgatatt
    11581 gtccgatttc tgataaatcg agcatattgc cttccacaaa ttggatcgat gcgtcttctt
    11641 catatgctcg ctggctggca atcatcaaca tctcttctga aaggtctaac cctgtcactt
    11701 tatatccatc ttttgcaaag gcaaccgcta atgctccagt accgcatgcc agttccaaaa
    11761 tattttttgt gtcttcttgt aaatgacgtt tcgaaaaagc taaccattgc tgataaagtg
    11821 tttcgtccat cacttcatcg taaatgaaag caaatgtttc ataagccata tgttaatcca
    11881 accattcact aagatcgact agcggagcat ctgaccatag tttttccaga ttatagaaat
    11941 ttcgttctgg cgcatggaaa acatgtacga tcacatctcc aagatcgatc aatacccatt
    12001 ttccgccttc ttttccttca atacgtttta cttcatattt gttttcttct tctttatcaa
    12061 tgatttcttc tgtaattgcg ttgatttgac gttcgctatt tgctgaacag atcatgaaat
    12121 aatctgccaa caaggaaaca tttcgaacgt ctaaagcgat aatgtcttct gcccgttttg
    12181 aatctgctgc ttcaatcgca atttttaatt gttctttact atctatgatg atttcctcct
    12241 aatatgagca gcttattttt ttgctaccca gtaattatat gtttcgattg tttttggata
    12301 aatctgttgt tcttgcgcaa gcaaatgggc caatgtatgt tttgtttcaa atgctacagc
    12361 ttcgtctaga tcagcccatg caatggctcg tgcatcttgt accccaggaa aatctcgacc
    12421 tggttcaata tagtctgcta cataaatgat tttgtcaagg agactcatct tagcagcccc
    12481 agtcgtatgc acccggattg catgaagaat ctctgcatcg gtgattccta attcccgctc
    12541 tacaaacgaa gcgccaacaa gaccatgcca gattgcatta ttataattca ataactccgg
    12601 atcaaaaccg tctcttacga ttaccatttt gaactcctca tctggtcgtt cttttgcata
    12661 atcatgtgtc aatgcagcaa tactagcttt ttcaggagaa gcaccgtatt ttttggcaag
    12721 agccacggct gtttcttcta ctccaagcac atgcttgaag cgttgttcgc tcattctcat
    12781 ctgtattttt tgcatcaggt tttctctaga ataagcagta tattcattac tatagtccat
    12841 attcatacaa cccttcattt tgaatataat caattacttt atctggaaca aggtagcgga
    12901 tagaacagcc ttctttgatc ttttgacgta ttttcgtgga actgatgtcg atctcaggta
    12961 catctaccca aattacagga tatggagtat ccgttgtgta tcctggtcga cgtataccca
    13021 caaagttgac catagaagtc agctcatcga ttttatacca ttttggcaaa tattctacca
    13081 tatccccacc gataataaag taataatctg tatccggatt gttttgcgtc aattccttca
    13141 tagtatcata ggtgtaactt ttcccaccac gagctaattc aatcgtttca atctgcaaaa
    13201 aaggattgtc ttccaccgct aattccaaca tgttgagtcg atgttttgca tcaatcgtct
    13261 gtttctcatc aacatgaggt ggctgataag aaggcatcaa aaatacgcgg tcaagaccta
    13321 aattccggcc agcttgttca gccatcacta aatgtgcaag gtgaacggga ttgaatgttc
    13381 cgcccaagat ccctacttgc tttttcttct ctaagaattg aggcatttct tcaggaaata
    13441 attgtgaacg aacaaatgtc ttagcttgcg tattcatcat ggtcactcct tatattgctt
    13501 ttacttcttt tgatagcctt tgatactttt cttttgtgga aggtttgtat aatacaataa
    13561 cacgtccaat gatttggacg atttcgcaat ctatttcttt ttctaatact tgagctgctt
    13621 cttctgcaat ctcatctgta ttttgaagta acgatacttt gatcagttct cttttttcta
    13681 aagcttcttc aatttgaacg atcattgctg aattgatccc accttttccg atttgaaaaa
    13741 tcggctgaag atgatgcgct ttgctacgta aaaaacgctt ttgttttcct cttaaactca
    13801 taattttcct tcttccttaa atcaatgctt tgcgtttcaa tacatctaca ccctttggcg
    13861 cccagcccgc aattactact ggcgctgtta cagtgatcca gcctagaccg gcaaaaacga
    13921 tgtctgtttt ttctttcaca gaaaactcaa aacgaacaag ctccggaaat gcctcgactt
    13981 catcaggacg aggaggctgt aataatccac ctacatgttt ttgataaaat tcgtctgctt
    14041 tttccagttt cgttcgatga atatttacat catttgacac ataagcaata aatgaagcac
    14101 gattgccgct gataaaatca aatcttgcaa gtccgcccaa gaaaagtgtt tgcccttcat
    14161 tcagctgata aactttaggt ttgatttctt ttaacggtgc aatgatccgc aaatcttttt
    14221 tacctaagta gtgcgccatt tgatggcgat ggataattcc cggtgtatca atcaaaaaat
    14281 gcccgtcatc taaaggaatc tcgattttat ccaaggtcgt tcctggaaac tgcgaagttg
    14341 tgattacatc ttgtactccc gctgtatttt tgatgatttg attgataagc gtcgattttc
    14401 cgacatttgt tactccgacg atatagacat cccgtccatc gcggtattct tcgatttttt
    14461 ctaatagttc cgtcatctca tgagatttct tagcactagt taataataca tctactggac
    14521 gaattcctgc ttcgtaagca cgttccttca tccattgtgt taatttagtt tttttcaatg
    14581 atttaggtaa tatatccact ttgttcccaa ctaaaagtac tggattgtca ccgacaaaac
    14641 ggtgtagtcc tgggattagt gaaccattga agtcaaaaat atccaccaca ttgacgatca
    14701 atgcatcttt ttgccctaat tcattcagta agcgcaaaaa gtcgtcatcc gtcaatgaga
    14761 catcttgggt ctcattataa tggcgtaatc tgaaacaacg ctgacaatag acttccccgg
    14821 aagctaaacc tttttccaaa gccgtttgcg gcgtgtatcc taattcaccg ggtttgtttg
    14881 tttgtataac ggcaccacag ccgatacatt gtaattcttc tgtcattcga ttccacctcg
    14941 ccagcccata tcagggtgtt ttgctaatag ataggccata attctttttt cccaaaaacg
    15001 attaaattgt gtcttccaac tatctgttgc gataatgggt tttacaagga tgctacgtat
    15061 ccctgcacga ttggctccac gtatatcagt cataatctga tcgcctacca ttactatctc
    15121 atcatctgct aaatccaatt gtttctttgc tatgttgatc cctcttgcta aaggtttcat
    15181 tgctcgtgca acatacaaaa gatcaaattt ttcaatcgct cgcgcgacac gttttgaatt
    15241 attgtttgaa acaacaacaa cagggattcc tgcatttttc atttctaaaa tccaagtaag
    15301 caactcttct gttccatctg gattattcca tgcaatcaac gtattgtcta aatcggtcaa
    15361 tactgctttg atgcctagat tctttaattg ggcaggtgta atctgataga tggcttctac
    15421 catccatgtt ggtttgtaat ttgaaaacat gctgtctcct catctgcaaa aaatgaagga
    15481 gcacataaag tactcctcaa ccccgtatta tactgcatat cagcaaaaaa aactacctct
    15541 atactcgttc gtctttcaga aaaagcattt ctttacaaaa tgaccaaaaa taaagattga
    15601 tcacagaaaa atttagaaac aaaaaacaac taatattggc ggaacctctt gtatcgtttt
    15661 tccagtaatt tatgctgatc taatcgaaca agttccgtca atctttcaat caacgctttt
    15721 ttcataatgt gtgcgacttt atatcgtggc aactgctcgc cgttgaacgt ttcaggaata
    15781 actcgatcaa cgattcctaa ctcgcgtagt tctggcgcag taattttcat caactcagct
    15841 gcttctgcgg cgcgactacc gtctttccac aaaatcgacg caaatccttc tggagaaagc
    15901 acagcataaa tcgtttcttc cagcatccag acttcatccg ctaaacctag agcaagggca
    15961 ccgccacttc caccttcacc aatgatgatg gcgatgatcg gtacctttaa atcagacatt
    16021 tcgagcagat ttctggcaat agcctctcct tcgccgcgct cttctgctcc tacaccgcaa
    16081 taagcacctg ctgtattgat caaggtaatc accggtctat tgaacttttc cgcttgtttc
    16141 atcagtcgta aagcttttcg atagccttca ggatggggag aaccaaaatt tctgcggata
    16201 ttttcctgca gatcccgtcc tttttgaata ccgatcactg ttaccggttt gccatctaat
    16261 cgagcgattc caccaacaat cgcttcgtca tctgcaaata aacgatcacc atggcattcc
    16321 tcgaaagaat cgaagatctc ttcaaaaaaa tccaagcttg tccatcgttc tttagctctt
    16381 gctaactgaa cgacatcatg tgctgtctta tccacgatct atccatcctt tctgtgtatg
    16441 caattcaaga agcgtatgga ttttctgttt caattccatg cggggaacaa tctgatcgac
    16501 aaaaccatga gataataaga attcagcctt ttggaaatct tctggaagtt cctgcttgat
    16561 cgtctgttcg atgacgcgtc ttccagcaaa accgatcaag ctttggggtt ctgctagaat
    16621 aatatcgcct tccattgcaa aactggcagt tactcctccg gtcgtcgggt ccgttaaaac
    16681 agttagataa aaaagacctt cattgctatg ccgtttgaca gcagcagaga tttttgccat
    16741 ctgcattaga gaaaagattc cttcttgcat ccgtgcgcca cctgaagctg tgaacaagac
    16801 gactggaaga ctttcctttg ttgctcgttc gaataatctg gtgatctttt ctccgacaat
    16861 cgttcccata cttcccatga taaaattagg atccatcact ccaataatga agggaatccc
    16921 ctgaatggtt gctttaccag tgaggactgc ttcatgaagt cctgttttat cttgcatttt
    16981 tttaattttt tccgtatatt cgggaaatgc tagcggatct ttggttacaa gatccgtatc
    17041 ccattcttca aaactttttt catcaataat caaagctaga cgctgccatg caccgattcg
    17101 aaaagcgtat ccgcaatgag ggcatacttt ttcttcaccg atgtctttcg tataaaggat
    17161 atgtttgcaa tttggacact ttgcccacat gttatcagga acagatggcg agttagcagg
    17221 tttgctctct ctattaggat tgatccgaat atatttcttt ttcttaaaca gagccatcta
    17281 tgcgcctcct atctccgttt ctggtgtcca attcggtaaa aattcttctt gtaaaaatgc
    17341 tgtgctgtaa tcacctgcta tgactgcagg atgactaatc aaatccatct gaaattctgc
    17401 gttcgtgatg attccttctg tgataaattc acttaaggct cgctgcatct tcattaatgc
    17461 ttcaaaacgc gtatttccat ggacgattat ttttgcaatc attgaatcat aatatggcgg
    17521 gatcgtatat cccgggtaaa ctgcactgtc tactcgcagt cccatcccac ctgctggaag
    17581 cagtaagttt tggatcttcc ctggagaagg agcaaaatgg aaagcaggat tttctgcatt
    17641 aatccggcat tcgattgcgt gtccttggaa tactacttct tcttgacaca cattcaaagg
    17701 ctcaccggct gctatctcca cctgtttttt tactaaatcg attcctgtga ccatttctgt
    17761 tactggatgt tccacttgga ttcgagtatt catttccatg aaataaaaat cgccttcttc
    17821 atccatcaga aactcgatcg ttccggcatt ttcataattg actgctttag ctgctcgaac
    17881 agcagcgtct cctagtgctt ctctttttgt ttgtgagatc actacagaag gagattcttc
    17941 cagtactttt tgattgttgc gttgtaaaga acaatctcgc tcgcctaaat gaatgacatg
    18001 accaaatcga tcacctaaga tctgaacttc tatatggcga gcaggataaa tgattttttc
    18061 caaatacata tcatcatttc caaatgccgc tgctgcttct tgctgagctg aagaaaaatg
    18121 ctgtgggagc tcttcttttg attgaacttt ccgaatgcct ttcccgccac cacctgcagc
    18181 tgctttcagc atcactggat aaccaattct gtctgctatt tccaatgctt cttctactgt
    18241 agataataca ccagtacttc ctggaatgac tgggacattt gcttcttgca tcaattgacg
    18301 cgcattgatc ttatttccca ttgcatcgat cgttgcactt ttaggaccaa taaatgtgat
    18361 attgcactct tcacacatgg aggcaaatcg gctattttca gataaaaaac caaatcctgg
    18421 atgaattgct tcggcttttg tcacgattgc agcgctcagt acttcctgaa cattcagata
    18481 tgaatcaacg gcttttgccg ggccaatgca gattgcttca tctgctaact gtgtatgaag
    18541 tgcttcggca tctgcttctg aatatatcgc tacggtttgg atccctagtt cgcggcatgc
    18601 acgaatgata cgtacagcga tttcaccgcg gttagctatc aatactttag aaaacatatt
    18661 tcacctatcc aatcataaag gttaattccg cttcggctac ttttttaccg tctactttag
    18721 cgattccttt gcctattccg gctgaagctt tcactttcaa tatctcaact tcaaggtata
    18781 aggtatctcc cggtgtaact ttcttacgga attttgcttt gtccaatccg ccgaaataag
    18841 ccgtctttcc cttgaattgt tcaagagaaa gtaatgcaac tgctcctgct tgagccattg
    18901 cttcaacaat caatacccct ggcataacag gttcttctgg aaaatgtcct tgaaaaaagg
    18961 gctcattgat cgttacattt ttcttagcaa cgatccgttc tccttcaacc atttcttcca
    19021 ctcgatcaat caaaagcatt ggataacgat gtggaatgat ttcttttatt tcttgtatgt
    19081 tcattgtttt ccctctttcc tgctttgctt atttttgctg aatcctaaag agtggctgtc
    19141 catattcgac aactgactcg ttttctacaa ggatttctgt gatcactccg tcggcctcac
    19201 tagttatctc attcatcagt ttcatcgctt caataatgca gataacttct cctttggcaa
    19261 cttgatcacc tacttttttg aaagctggtt tgtcgggcgc tggttgcaga taaacgattc
    19321 ccacgattgg cgaaacaacc tcgattccat caaatactgc cgattctttt tctttcatta
    19381 tttctggagc agttgttgct tgttcaggta tgacattgac tggctccgaa actggcaacg
    19441 tttcgtttgt ttgaaccata ttcatgcctc gacttgtttt atttttattc atatacaatt
    19501 caaattgacc ttcacgcaaa tcgaattccg ttagcgagga ttgatcaaat tgactgacta
    19561 attctctgat ttcattgata tccaaaatta ttcctcccag cgtttcatgc acagaaccgc
    19621 attatgtccg ccaaacccta atgaattact cattgcataa gtaaaacttg caggtttgct
    19681 ttcgtttttg acaactgtca agtcaatctc aggatcttgt ttttctacat tgatcgtagg
    19741 tgggataaat tgatgttcta atgccagcaa agttgccaag gcttcgatcc cgccggctgc
    19801 tcccaataaa tgtccagtca tcgatttcgt gctgctgacg taagtatctc tgaaattttc
    19861 acctaatgcg atttggattg cttttgcttc tgcctggtca ttggctggtg tacttgttcc
    19921 gtgagcattg atatatccga cttgttccgg ctgtacacca gcttcttcca tcgcaagctt
    19981 cattgcttta gatgcaccag aaccatctgg agtaggagct gtcatatggt aagcgtcaca
    20041 gtttgcaccg tatccaacga tttctccata gattttggca ccgcgagctt gtgcatgttc
    20101 taaagattca agaactagaa cgcctgctcc ttcacccatc acaaaaccat tccgattttc
    20161 gtcaaacgga atcgaaccac gattaggatc agttgattct gagagtgctg ttaaagctgc
    20221 aaatccagca atccccatct cacaaatcgt tgcttctgtt cctccagcaa gaatgacatc
    20281 agaatagcca tgtttgatgt tacggaatgc ttccccaatt gaattcgttc cgctagcaca
    20341 agctgttacg atagctgtac aaataccttt tgcacctaca cgcaaagcaa tatttccagc
    20401 tgccatgttg gcaatcgcca ttggtacgaa cattggagaa actcgttttg cacctttttc
    20461 atgtagacga ataacttggt tttcgattgt tggaagacca ccgatcccag atccgatgat
    20521 gacgccaaac cggtcttgat tcattttttc tttatctaaa ccgctcatct ccattgcttc
    20581 taaagcagcg tagattccat agatcgaaaa catatccatt cgtttgctat cttttttaat
    20641 aaaatattta tcgtaaggga aatctttgac ttcacccgct aaggtaatcc ctgtttctga
    20701 agcatcaaat ttagtgatcg gtgcgatgcc attcgtcccc tcttttaagc tattcaaaaa
    20761 gctgtctgcg tcatttccaa taggtgaagt gactccatag ccagtaatta ccactcgatt
    20821 catttgtttg ctctcctttt cttatccgtg catgaccaat ccgccatcga cattgatgac
    20881 ttgtccggtg atatatggat tttgtgctaa gaaaactgct gtttgtgcaa tatcttctac
    20941 ttgtccgaaa cgctgcatcg gaatttgctt ttccgcttgt tccttgactt tgtctgctaa
    21001 aacttccgtc atatcagttg tgatgaatcc tggcgcaatg gcattacatg taatgcctct
    21061 agttgcagct tctcgtgcca cagattttgt taatcctact acgccagctt tactagctgc
    21121 ataatttgct tgaccaatat ttccaatcaa cccagataca ctagagagat taatgatggc
    21181 cccttctcgc tgcttcatca tttttttcaa tacgtgctgc gtcatattga aggttccaat
    21241 caaattgata tccagacatt ttttgaaatc ttctgcatcc attcgcatca caagtttgtc
    21301 atttgtgatt ccggcattgt ttactaacac atggatcgat ccaagctttt cttctgcttc
    21361 tttgatcatt tgtcccgctt tctcataatc agagatgtca ccggaaactc ctacgcattt
    21421 tacaccgaat gcttcgattt cttctatctt ttcttttgga atctctccgc gaccattcaa
    21481 aatgatattt gctccagctt ttgcaaaagc taaggccatc gctttgccga ttcctcttgt
    21541 actacctgtg ataaatacat tttttccttt tacatccata acgttcctcc ttatagtatt
    21601 gctattgttt catcaaatgt ctgcttattc tcaacacgca gaactggtat tgttttatcg
    21661 atttttttca taaatccgct taatactttt ccggggccga cttcgatgac ttgttcgatg
    21721 ccgattgttt ttaacttatg gatactctca tagaaacgaa caggttgcat gacttgctgt
    21781 tcaagtagtc ccgcgattgt ttctttcttc attatttccg ttgttgtgtt gcttatgata
    21841 ggaaaagaag gttcagagaa ttgaatctga cttaggtcct tcgccagttt ttttgctgcc
    21901 ggttctaaaa tagctgtatg aaaaggcccg ctgacattta gagggatcat tcttttgaac
    21961 cctttttctt ttaatagtaa aacagcttcg tctactgctt tttcttcgcc accaatcacg
    22021 atctgctgag gtgtattata atttgcagga gatacgatgc cgtacttgct agcttcatga
    22081 cagctttcct cgatcgtttc aatcggcgca ttcatcactg caaccatttt cccgcttcca
    22141 gctggggcag cttctgtcat ataagcgcct ctttttgcta ctaacgcaac tgcttcagtg
    22201 aaggatagcg cattactagc tactaatgcc gaatattctc ctaaactcag tcctaaagct
    22261 gcatctggga tgattccatg ctccttcaat aagcgataat acgcgatgct gactgttaga
    22321 atagctggct gtgtatattg cgtctgatcc aaacgttcat tttctgtaaa gcaaagttca
    22381 gccatatcgt agccgagaat ctcacttgct tcatcaaatg tctgctttac aacggcttct
    22441 tcatataaat cttttcccat accttgatat tgtgcacctt ggccactaaa taagaatgct
    22501 gttttcatgt ttttctccaa tatatccaga atatatgttt gattcttcct attcaaaaag
    22561 gccgagacat tcctatttca ggaaatgtcc cagcccccaa cctaatttta atgattatgc
    22621 agtttgtttt tctacgtaag tcactaaatc ttgaaccgtt ttgattccgt cttctgtttc
    22681 gattttcacg tcaaattcat cttcaatttc attgatgatt tggaataaat ccaagctgtc
    22741 agcttctaag tcttcttgga tgtcagtagt caatttgatt tcttcagttt cttttcctaa
    22801 ttcttctgcg atgatttctt gaattttttc gaataccata ataatatatt cctccgatat
    22861 tgtttgcttt ttatttttat aatttaacag tctacaactc catgagaata cttccccatg
    22921 ttaaacctcc gccatagcct gtaaatatta ctttctgctg ttttcctaag gtgatctttc
    22981 cactagaaac tgcttcatcc aataagatag ggatgcttgc agcagatgta ttcccatatt
    23041 tatccatatt ggtcaggaac ttttcttgcg gcatctttac ttttcgggca attttctcaa
    23101 taattctgag gtttgcctga tgcaaaagca gataatcaac tggtttgtct ttcatgactt
    23161 cttggatatt gtttgcaacg tctcgtacgg caaaatcaaa aatatctctg ccagtcattt
    23221 gcaaataata cgaaccttct gaatcttctg aatagaacgg atttttattt tgctggtagc
    23281 cagcggtcaa tgagctactt ctggtaccgt cagctgctaa tttttctttc agcaattttt
    23341 tctgtggatt cgcttctaag agaatcccac ctgctccatc gccaaacaac acagcagtcg
    23401 tacgatcaga ccaatcgatt agcttagata gtgtttcacc accaatcacc agtcccttag
    23461 ttgagccgca gcggatcatt ttttcagcca tgcttaacgc ataaacaaat cctgagcacg
    23521 cagcactgat atcaaaagcg atagcatgag ttgctcccaa tttcccttgt acttgtgctg
    23581 ctacagatgg tgtcgtgtaa tcgggcgaca ttgttgctac taaaataaaa tccaaatctt
    23641 tagggtttag atttttctgt tttatcaatt tttccgccac taaaatgcat aaatcagaag
    23701 tctcctgatt tgttacacat cttcgttcac gaatacccgt ccttgacgaa atccattcat
    23761 cagaggtatc catcatcttg gaaagatcat tattagtaat aatcttttca ggaaggacgc
    23821 tggctgttgc agtaatcatt ccaaattgtt ccattaaata tcctcactta tagtcttgca
    23881 agaagtcgtg caagtttttc agtgcatcca gcaaggcatg ctgttcctcc gtagccattc
    23941 cgtctaagat gttcttgacc atttcacgat gaaaatgctg atggacccga aatagtaact
    24001 ttcctttttt cgttagtccc aactttacaa cgcggcggtc atcttcactg cggatacgtt
    24061 cgacatagcc tttttttact aacttgttaa tagcagtggt caaggtccca acagtaattg
    24121 atagttcttt ggctacttct gaagttgttt ttttcttgta catcccgatt gcctcgatcg
    24181 tgtgcatctc tgtgattgac agatctttga attgagattt tttcagctca gactcttcga
    24241 ttgtcaaaat atcattgaag acactaacaa gataatcatt tactgttttt aaatctggtt
    24301 ccatgttcca ccgtc
//
LOCUS       DAYYCW010000035        24259 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000035 DAYYCW010000000
VERSION     DAYYCW010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.035"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHAYA_001285"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHAYA_001285"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM8605093.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHAYA_001286"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHAYA_001286"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM8605094.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHAYA_001287"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHAYA_001287"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM8605095.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="ACHAYA_001288"
     CDS             complement(3373..4680)
                     /locus_tag="ACHAYA_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM8605096.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="ACHAYA_001289"
     CDS             complement(4683..5264)
                     /locus_tag="ACHAYA_001289"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM8605097.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="ACHAYA_001290"
     CDS             complement(5836..7128)
                     /locus_tag="ACHAYA_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8605098.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="ACHAYA_001291"
     CDS             complement(7125..7748)
                     /locus_tag="ACHAYA_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605099.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="ACHAYA_001292"
     CDS             complement(7751..8587)
                     /locus_tag="ACHAYA_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8605100.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="ACHAYA_001293"
     CDS             complement(8604..9491)
                     /locus_tag="ACHAYA_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8605101.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="ACHAYA_001294"
     CDS             complement(9507..10790)
                     /locus_tag="ACHAYA_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8605102.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="ACHAYA_001295"
     CDS             complement(11473..12156)
                     /locus_tag="ACHAYA_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8605103.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="ACHAYA_001296"
     CDS             complement(12174..12791)
                     /locus_tag="ACHAYA_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8605104.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="ACHAYA_001297"
     CDS             complement(12801..13625)
                     /locus_tag="ACHAYA_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM8605105.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="ACHAYA_001298"
     CDS             complement(13618..14178)
                     /locus_tag="ACHAYA_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605106.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="ACHAYA_001299"
     CDS             14352..15308
                     /locus_tag="ACHAYA_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8605107.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="ACHAYA_001300"
     CDS             complement(15613..16569)
                     /locus_tag="ACHAYA_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM8605108.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="ACHAYA_001301"
     CDS             16673..17275
                     /locus_tag="ACHAYA_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8605109.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="ACHAYA_001302"
     CDS             complement(17363..18862)
                     /locus_tag="ACHAYA_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM8605110.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="ACHAYA_001303"
     CDS             complement(18878..19402)
                     /locus_tag="ACHAYA_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM8605111.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="ACHAYA_001304"
     CDS             19700..20449
                     /locus_tag="ACHAYA_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8605112.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="ACHAYA_001305"
     CDS             20440..21225
                     /locus_tag="ACHAYA_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8605113.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="ACHAYA_001306"
     CDS             21194..21910
                     /locus_tag="ACHAYA_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8605114.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23031)
                     /locus_tag="ACHAYA_001307"
     CDS             complement(22012..23031)
                     /locus_tag="ACHAYA_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM8605115.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23050..24051)
                     /locus_tag="ACHAYA_001308"
     CDS             complement(23050..24051)
                     /locus_tag="ACHAYA_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM8605116.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5222 c         4225 g         7624 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg tattaacatt
    24181 tttaatttta tctttacatt gttacttatt gaacaatagg aaattaaaga aacttttgaa
    24241 acttatgtaa agtgatttt
//
LOCUS       DAYYCW010000036        24156 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000036 DAYYCW010000000
VERSION     DAYYCW010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.036"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..842
                     /gene="purR"
                     /locus_tag="ACHAYA_001309"
     CDS             24..842
                     /gene="purR"
                     /locus_tag="ACHAYA_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM8605117.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1345..2718
                     /gene="glmU"
                     /locus_tag="ACHAYA_001310"
     CDS             1345..2718
                     /gene="glmU"
                     /locus_tag="ACHAYA_001310"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM8605118.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2812..3783
                     /locus_tag="ACHAYA_001311"
     CDS             2812..3783
                     /locus_tag="ACHAYA_001311"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8605119.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3789..3891
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3957..4526
                     /gene="thiT"
                     /locus_tag="ACHAYA_001312"
     CDS             3957..4526
                     /gene="thiT"
                     /locus_tag="ACHAYA_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM8605120.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4607..5380)
                     /locus_tag="ACHAYA_001313"
     CDS             complement(4607..5380)
                     /locus_tag="ACHAYA_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605121.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5647..7347
                     /locus_tag="ACHAYA_001314"
     CDS             5647..7347
                     /locus_tag="ACHAYA_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM8605122.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGRGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7317..7535
                     /locus_tag="ACHAYA_001315"
     CDS             7317..7535
                     /locus_tag="ACHAYA_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM8605123.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7555..8445
                     /locus_tag="ACHAYA_001316"
     CDS             7555..8445
                     /locus_tag="ACHAYA_001316"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM8605124.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8463..8747
                     /locus_tag="ACHAYA_001317"
     CDS             8463..8747
                     /locus_tag="ACHAYA_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605125.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            9234..9848
                     /gene="gmk"
                     /locus_tag="ACHAYA_001318"
     CDS             9234..9848
                     /gene="gmk"
                     /locus_tag="ACHAYA_001318"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8605126.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            9853..10158
                     /gene="rpoZ"
                     /locus_tag="ACHAYA_001319"
     CDS             9853..10158
                     /gene="rpoZ"
                     /locus_tag="ACHAYA_001319"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8605127.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            10411..12819
                     /gene="priA"
                     /locus_tag="ACHAYA_001320"
     CDS             10411..12819
                     /gene="priA"
                     /locus_tag="ACHAYA_001320"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM8605128.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            12834..13325
                     /gene="def"
                     /locus_tag="ACHAYA_001321"
     CDS             12834..13325
                     /gene="def"
                     /locus_tag="ACHAYA_001321"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8605129.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            13318..14256
                     /gene="fmt"
                     /locus_tag="ACHAYA_001322"
     CDS             13318..14256
                     /gene="fmt"
                     /locus_tag="ACHAYA_001322"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM8605130.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            14256..15614
                     /gene="rsmB"
                     /locus_tag="ACHAYA_001323"
     CDS             14256..15614
                     /gene="rsmB"
                     /locus_tag="ACHAYA_001323"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM8605131.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            15627..16367
                     /locus_tag="ACHAYA_001324"
     CDS             15627..16367
                     /locus_tag="ACHAYA_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM8605132.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            16364..18433
                     /gene="pknB"
                     /locus_tag="ACHAYA_001325"
     CDS             16364..18433
                     /gene="pknB"
                     /locus_tag="ACHAYA_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM8605133.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            18598..19497
                     /gene="rsgA"
                     /locus_tag="ACHAYA_001326"
     CDS             18598..19497
                     /gene="rsgA"
                     /locus_tag="ACHAYA_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM8605134.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            19510..20160
                     /gene="rpe"
                     /locus_tag="ACHAYA_001327"
     CDS             19510..20160
                     /gene="rpe"
                     /locus_tag="ACHAYA_001327"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM8605135.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            20161..20799
                     /locus_tag="ACHAYA_001328"
     CDS             20161..20799
                     /locus_tag="ACHAYA_001328"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM8605136.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            20954..21808
                     /gene="mreC"
                     /locus_tag="ACHAYA_001329"
     CDS             20954..21808
                     /gene="mreC"
                     /locus_tag="ACHAYA_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM8605137.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            21812..22321
                     /gene="mreD"
                     /locus_tag="ACHAYA_001330"
     CDS             21812..22321
                     /gene="mreD"
                     /locus_tag="ACHAYA_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM8605138.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            22576..24150
                     /locus_tag="ACHAYA_001331"
     CDS             22576..24150
                     /locus_tag="ACHAYA_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM8605139.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         8113 a         4391 c         5197 g         6455 t
ORIGIN      
        1 tttcttaaaa ggagttggaa taggtgaaag tacgtcgcag tgaacgtcta gttgacatga
       61 cacagtattt attgaatcac ccgcatgaat tgatttcctt aacaagcttt gccgaacgtt
      121 atgaatctgc taaatcttct atcagtgaag atttgggaat catcaagaaa acatttaaag
      181 aacgagggta tggaaccttg gaaacaatcc cgggtgctgc aggtggtgta ttgttcatcc
      241 ctgaaatttc atatgaagag gcgaaaaaat atattgaatc tctagcagaa cgtctatcag
      301 aacaagaccg tctgcttcca ggaggatatg tttatctctc tgatttgtta ggagaacctg
      361 aactgctgcg tcaagttgga agaatcattg cttctaaata ccttggtgaa aaaatcgatg
      421 ctgtaatgac tgtagccaca aaaggcgtac ctattgccca agctgtgtcc tattatctga
      481 atgcgccatt cgttattgta cgccgggact caaaaataac agaaggatca acagtaagtg
      541 tcaactatgt gtctggatct tctgaaagaa ttgaaaagat ggaactgtct aagagaagtt
      601 tgaaacgtgg ttcacgtgtc cttgtagtgg atgatttcat gaaaggcggc ggaacagtca
      661 acggaatgaa gagcttgatt gaagaattcg aatcagaact tgttggcatc actgtatttg
      721 ccgaatcaaa attcaacggt catcgcgcca tcgatgatta tacgtcactg ctttatgtca
      781 aagatgtaga tactcaaacg aaaaatatta cggttgtgcc aggaaattat ttttcagaat
      841 aagaggttgt gaaaaagccg cttagtttcg agtagtaaga aaaattttgt gaaaatggct
      901 ttccacattt tttcataaat ttggcttaat accgagaaac tggcttttga acaccgttta
      961 tctagaggtt gtgaaatcag cgtcctgtcc tgagtattag ctcaataaac ggaaaaattg
     1021 ctcctcatat tttgccgttt attgagctaa taccgaaggt cagctgattg aaaaacgttt
     1081 aatgaagagg ttgtaagcct accgtttaac tctaagtcac aagaaacgat ttcaaaaaac
     1141 agcttttcat gttttatgag tagtgtgact tgtgaccgag gagttggcag gagagcaccg
     1201 gttaccaaat agataggccc gttatggtct atctattttt ttatccagaa aacgctggga
     1261 taaaagaaaa tcacttgttt tgttgaaaaa atgggtatac tgggataaac tgaaatcagc
     1321 aaactatatt taggagtgac gcagttggac gcacgttatg caattatttt ggcggcaggg
     1381 aaaggtaccc gcatgaagtc aaaattatat aaagttttac atcccgtttc tggtaaacct
     1441 atggtagaac atatcattaa tcgggtcagt gaaacgaaac cagatgaagt gattacgatc
     1501 gttggacacg gagcagaaca agttaaagca caactaggag aacgcagtaa atatgcgtta
     1561 caagcagaac aattgggaac aggacatgct gttttgcaag ctgcttcttt cttggaagga
     1621 aaaaaaggaa caacgttagt tatcagtggc gatacaccat tattgactac tgaaacgctg
     1681 aacaatcttt ttgaatatca ccaaggaaaa aatgcaagtg caacgatctt gacggcacaa
     1741 gcagaaaacc caacaggata tggacgaatc atccgtgacc atattgggat cgtagaaaaa
     1801 atcgtggaac aaaaagatgc aacaccagaa gaagctcttg tacaagaaat caatacagga
     1861 acttactgtt ttgacaacga agctttgttc gatgcgttaa gcaaagttgg aacaaacaat
     1921 gctcaaggtg aatactatct aacagatatc atcgaaatct taaaagaaga aggccataca
     1981 gttgctgctt atcaaacgga tgattttgaa gaatcaatgg gagtcaatga tcggatcgcg
     2041 ttagctaaag caaatgagat catgagaaag cgaattaacc agatgcatat ggtcaatggc
     2101 gtaagctttg tcgattcagc aacgacatac atcgatgcag gcgtagaaat cggtccagac
     2161 acattgatcg aagcaggtgt acaaatccaa ggaaatacag tcattggttc tgattgtgtc
     2221 atcgggtcac attcaaaaat cgtagacagt aggattgagg atcacgtcgt cattgaaaac
     2281 tcagtcattg aaagcagcca tgtcaagaaa catgcagatg tgggacctta tgcacattta
     2341 cgaccaaaag ctgaaattgg cgaaaacgtc catatcggaa actttgtgga agtgaaaaat
     2401 gctcaaatcg gcaaaggaac taaagtcggt cacttgactt atgttggtga tgctacgctg
     2461 ggagaagaaa tcaatgtcgg atgtggtgtt gtcttcgtca actatgatgg aaagaacaaa
     2521 catcatacga ctgtcggcga ccacagcttt attggttcta gcacgaatat catcggacct
     2581 gtcgaggtag ctaaaaacag ttcgattgct gccgggtcaa caattacaga caatattcca
     2641 gaatatgcgc tggcaattgc gagagcaaga caagtcaata aagaaggata tgcaaaaaaa
     2701 ctcccttatc taaactaaga cttttgcaaa atcacttgat ttatttaaaa aatatgacta
     2761 ctatttatta ggtagaattc tagagttgtg aaaaagaaag tggaggtccc catgtcaaaa
     2821 cattactttg acccaagatt aaaaattttt gcgttaaatt ccaatcgccc attagcagag
     2881 aaaattgcag cagcagtcgg cgtcgagtta ggaaaatcat ctgttactca atttagtgat
     2941 ggagaaattc aagtaaacat tgaagaaagt attcgtggat cgcatgtcta tgttattcaa
     3001 tcaacgagca gcccagtcaa tgataacttg atggagttgc tgatcatgat tgacgcactg
     3061 aaacgagcaa gtgcaaaaac aatcaacgta gtcatgcctt attatggcta tgcgcgtcaa
     3121 gaccgtaaag cacgtgcacg ggaaccaatc acagcgaaat tagtagcaaa catgattcaa
     3181 aaagctggtg caacaagaat gctgactttg gaccttcatg cagtccaaat ccaaggtttc
     3241 tttgatattc cagtcgatca tttgatgggt gcgccactta ttgccaatta tttcttagaa
     3301 aaaggaatca aaggcgatga tgttgtcgtt gtttcaccag accatggtgg tgtcacacgg
     3361 gcacgtaaat tagcggaatt tttgaaatct ccgattgcga tcatagacaa acgccgtcca
     3421 aaagcaaatg ttgcagaagt aatgaatatc attggacaag tcaaaggcaa aacttgtgta
     3481 ctgatcgatg atatgatcga tacagcaggt acgatcacat tggctgccaa tgctttgaaa
     3541 gaagctggtg caactagtgt gtatgcttca tgtacacatc cagtattatc cggccctgct
     3601 ttacaaagaa tagaagattc agctatcgag cgtctagttg ttacagactc gatctatctt
     3661 ccagaagatc gtaaaatcga caaaatcgat gaagtaagtg ttggcggact aatgggagat
     3721 gcaatcaaac gtatccatga aaacaaacca gttagtccat tgtttgaaac aaaaaataaa
     3781 taataaataa actcacaaag gggagtccaa ttgggctgag attgaatcta tttctaaacc
     3841 cttcgtacct gtatcggtta tgcgagcgta ggaattgtga ataaacagct gtttgactat
     3901 tttcgaaagc tgcttcctcc tttgtgactt ttttattcaa aggaggaagt ttttttatgg
     3961 gaaaaaatag agtttgggta gaaggaacga ttgttgcagc gttagcgatg gtgttgtcgt
     4021 tgattccgat ccagatcggc agcagttttt ctatttcatt agggcagatt ccattgactc
     4081 tttttgcgtt aagaagaggg acaaaggcag ggatgctggc aggtttcatt tggggcatcc
     4141 tacattttcc gttgggacaa gtttattatt taagtgtcgt tcaagtgttg attgagtacc
     4201 cgattgccta tacttttgca ggcgcagcag ggctcatggc ttcaaatgtc caaaaaggct
     4261 atgtagacga taatacgaaa caaattagaa aaagcatcgt gtttggtgca ttgattggcg
     4321 cactttccag atatttttgg cattttattg caggagtggt tttctgggga gcgtatgcac
     4381 tatgggggat gaatccttgg ctcttttctt tcgtaatgaa tggtgccagc gggttgagta
     4441 cagctattgt aacgatcatc gttttattag ggatccaaca aacgactcct tcgatttttg
     4501 tgccagataa acgacaggag ctgtaaataa aaaagaagca agaaacttga gacagaatca
     4561 tgtcctagtt tcttgtttcc tttttatttt ttgataagaa atagatctac ttcaatggca
     4621 gataagaaag atcggcatct tctaccaatt gccagtgtcg atcggtgaca gagtagatta
     4681 aagtagcgat gatttcctct tctcgatacc gggtgccttt taatttccaa atataatctt
     4741 caatcacata gattgggata cgatatttac catcgatcaa accaacttga tcaaaagaaa
     4801 aaatgatccc ttcctgcatc aaattacgca tttctttcaa taattccaca ctgatttttt
     4861 caacaggtgt ttcttttttg aaaaaagatt tttttacatt gatcttatct gacaagatcg
     4921 aacttaattt actagcaaaa atcctataaa attcatctaa atagcgataa taaatccgat
     4981 tcagctgatc attaagatcg aagtatacaa aattattctg tagtttataa aaaaacggcg
     5041 aatgaagatg tgttttcata tgaccaaaat ataataattc tgaaatttct aaaggtgtca
     5101 attctttcag catcgttaaa tcagtaaagt cgatccactt caattcatcg aatccacgcc
     5161 tgttacgaga agaggaaaag aaacgctgaa tattttctgt accacggatt actttcagcc
     5221 ctgtatggga ttcatattct ccagcagaag cagatggatc aagcaagagc aagttttgag
     5281 gaaaatgcac gatactgttt gcaaagtcag tagcagtcag cccttttgtt aaaacagcat
     5341 tacttgtggt atcaatatgt acataaactg actcagtcat catgttcctc ctcttatttc
     5401 ctcttaatct cttttatttt acaacatttg cattaaaaaa gcttgtttct tttattttaa
     5461 aattccatta caaaatcacg ttttcattga aaacactctc attttcaatt aatttttgtg
     5521 atttttgtat ttttttattt aaaggaggtt ttatgaattg ttttcttatg gaatctacaa
     5581 agtcgaatac gagactcatg acctttttat gacgtactgg tatacttgaa gaaaaaggag
     5641 agcgcgatgg agataaaaaa agtttacttt tcacagaaaa tgtcgctgta ttcgcctatc
     5701 cagcaagcaa aacaacccct tccatttttt caaagtcatc aagaaagtag agacgctttt
     5761 tggcgttctg caaaaaagca atgtgctttt aaagcagtat cgaaaaaata taccgtctat
     5821 tttactattg ctaaaagtag aggagatgtt atttttaata atctgttgat tgaaggtgaa
     5881 acttttgaat ggaaaaaatt ttttcggtat atggaagctt gcgctcgttt ttttattaaa
     5941 gaaaactgtt gttttttatt tcaatctcct ctatcagaag aatggttgcg tatttttcat
     6001 aaaaacggct atcaaggaac cacacaatta aataaaaagc tggtctatca tacagcactt
     6061 gttttaggta gaggaggagc gcacggtgct tatcagattg gtgtgtggca ggcgctgaag
     6121 gaacatgata tcaactttga gattatcaca ggtacctcgg taggtgcgtt gaacggcgca
     6181 ttgattttac aaggagatat gaaaaaagct gtgaatttat ggaaaaagct gtcgacaaga
     6241 caagtgcttg cgttaccaga aatggctgca gttgaagacc ttcgtgaacg cttctatcga
     6301 gaaagacgcc aaatgacgaa aacagcacta attgaaggcg gggtttcttc tgctccattg
     6361 gaaaagatgg tcaaagacaa tttggatttg tccttactca aacataatcc caaaatacgt
     6421 ttatatacgg tgtctactaa actgccagaa ctagcggaag tagtgactga tatccaacag
     6481 acaaaaacag aagaaatacc agattggata ctcgcctctg cttcatttta tcccgctatg
     6541 gcttatcgaa agatcggaaa aaataaatat gcagacggtg ggtatcgcaa caaaatacct
     6601 attgatattg cgattaacaa aggagcaaca gaggcatttg tcgtagatgt gcaagggcca
     6661 ggcccagcga aaaaaattcg tatgccagac atttttatcc attggaaatg tcaaacattg
     6721 tggacactag gcagctttct gctttttgat agccaaagga accaattaaa tttacaatta
     6781 ggctatctgg aaatgaaaaa acgattaggc tattactatg ggaactggta tacatttcat
     6841 tctgttaaac ctgcaagaac atactggcga ggatttttgt cttacttaac aaaagaaatt
     6901 cagttagaat tatctttttt caaaagcata aaattttggc aaaaactgcg taatttatat
     6961 aagaacaggg tcgtaccaga gacatgcgga ttggcgatgc tagaactttt agcaaaaaaa
     7021 cattttttac tgcccaatga aatctatcaa gtagacatga tgattcaaat gatctgccaa
     7081 caagatataa aaagtatgcc ggatgattta ttggcacaaa ttggtcaatt aagtactgaa
     7141 gaatggcgaa ggtatcgaaa ataccagcaa aaaatacaaa atgagcgcac gaaaactgtt
     7201 tattttgatt atctgctaca aggaaaacgc aaggatcgtt tgcagcatga attgcttaag
     7261 caaccagttg acacattact gatcctatat ttatattatc tcaaggagga acaaccatgg
     7321 cacaagaatt ttcatatgaa atcgtagaag aaatcgctat attgtcagaa aacaacaagg
     7381 ggtggcgtaa agaactgaac ctagtcagct ggaatggtcg cccgccaaaa ttcgatcttc
     7441 gagattgggc gccagaccat gaaaaaatgg ggaaaggcct gacattgacg aacgaagagt
     7501 ttgaacaact gcaaaaagca atagaaaata tgtaatgagg aaggaccctg tcgaatgaaa
     7561 agtatgactg gttttggcaa agccatccgc gaaacaaaag cgtatcagtt agatattgaa
     7621 ataaaaagtg tcaaccagcg ttttctagat gttcaaatac gaagtcctaa aatcctgaac
     7681 tttgttgaaa acgattttcg tcagcttatt aaacagcagt taagccgcgg tcgaatcgaa
     7741 gtatttgtga atctagctta tattggcaaa aacgaaaaac agatcgtcat caactgggaa
     7801 ttgattgatt cacttatcac tcaattgaca gacggaattc aacaacgata cggtagtcaa
     7861 aatcaagtgt caattagtcg gatggcggaa cgcttcgtgc tggacgaaaa cttcgtaacg
     7921 gtagaagaaa aaccggaaga gagcatggaa gaattggcac agttagctct agatacattg
     7981 aaaagcgcac ttgattctat tgaggcgagt cgagaaaaag aaggtcaagc tttagcttta
     8041 gtgttgaaaa agaatactga agaattcaaa gaagtactaa aaaaactcga tagttttgta
     8101 gaaatctatg aaaaggaatt tcaaataagg tatcaaaaaa agctggagga atttcttgga
     8161 gcgaaagtcg atgagcaacg gttgttgacg gagctagcca tcttgttgga acgcggagat
     8221 attcacgagg aactggatcg tctggcgatc catgtacaaa aactagatga acttatcgaa
     8281 gcacaatgtc ctgtaggtcg ggaattagat ttcctgatcc aagaaatgaa tcgggaaatc
     8341 aacacgatcg gttccaaatc aaatgcaatc gaaattaaaa atcaagtcgt ccagtctaag
     8401 acgatcttag aaaaaatcag agaacaagta caaaatattg aatgacgaaa gaggtagaga
     8461 taatgaaata ttatgacgta acgttccacg aattaagtgg aaaaactgtg gttaaacggg
     8521 atatcccttc tgaaaaaaac ggctttgacg tatggaaaga tgcctgtgca gactataatg
     8581 agaacgaact gtttattttg atcaacgacg gtgcttacgt gacgatgaat cggaaattta
     8641 ttgttcgaat tgacacagaa gaggtagaag atccgacaga aaaagcacgt tctcgaaaag
     8701 atgagatcat gggtgtagtg aatactttgt ccaatatggg cttttagcaa ggatgctgaa
     8761 caaagaaata caataaataa acagaattgg atttcttcgc ttagcttctt tgctctagga
     8821 atacatttaa aggttgtgac aaaattcgtg tcacaatctt tttttatttt gaataagtga
     8881 tgttcaaaaa ccattttctc ggtattaagc caaatttatg caaaaatgtg agaaactatt
     8941 ttcacaaaat ttttcttcat actcgaaact aatcggcttc ttcacgacct cttatacacg
     9001 gcgttcaaaa gcttgcatct gcgataataa ccccaaccaa acgaaaaaca tgagaagctg
     9061 tttatgtttg gctgttctta ttacttgatg caggacagct tttttacgac ctcttggtac
     9121 agggtagctt ttttaccaat ttctagctat aattaaagat aaccttgaaa gttgcagaaa
     9181 aaaagtgcaa aataaacgat aagtacaaaa agactagttg aaaggacgtc gtcatgtcag
     9241 agcgtggatt attaatcgtg ttgtctggtc catccggtgt aggaaaaggc acagtacgaa
     9301 aagcaatttt tgagagtgag gataatgatt ttcagtattc gatctcaatg actacccgta
     9361 aaatgcgtga aggggaggtc gaaggcgttg attatttctt ccgaagcaaa gaagagtttg
     9421 aatcaatgat cgaagctgga gagatgctgg aatacgcaga atatgtcggg aattattatg
     9481 gaactccttt gtcatatgtc aacaagacat tggatgaagg aaaagatgtt ttcttggaga
     9541 ttgaagtcca aggagcaaaa caagtcaaag aaaaagtgcc cgatggtgta ttcatctttt
     9601 tgactccgcc agatttagcg gaattaaaat cacggatcgt aggccgagga acagatgcag
     9661 atgaagtaat cgaaaaacga atgaaagttg caaaagaaga aatcgaaatg atggctcttt
     9721 acgactatgc tgttgtaaat gatgaagtgc cgctagcagt tgaacgaatc aaaaacatta
     9781 ttgccagtga acattttcga gtagatcgcg taatcggtaa atatattaaa atgttaaagg
     9841 agatgtagtc ttatgatgtt aaaaccttca attgactcac tattagatcg tgtaaattca
     9901 aaatattctc tagtgatttt agcaagtaaa cgcgcgcatg aattggatgc aagtgcacag
     9961 cctactctag attcttttga ttcagtaaaa agcgtcggtc aagctttaga agaaatcgat
    10021 gcaggaaatg tagtcaatga tccacatcct gaattaaaac gtgaacgttt gaagatggaa
    10081 gaagaggaac gtcaagcaca aaaagagcgt gaacaacacg aattggaatc acgtatccgt
    10141 gaagagcaaa aaatgtaata aagaaaaata gagcgaacat ctaaaaagaa aagcaaaccc
    10201 agctgtttcc ttttcaaaca ttcgccactt attgtctggt agaaaaggct gtgtctgaac
    10261 tttttgacac agttcttttt tttcttatct gctgttaatt ttttgttagg atttggcaga
    10321 agataagaca aatccatcga tctctagtaa aataggcaag gaactttttg tcgcttgata
    10381 agaaaagaca aaaaaaggag gaaacgaacc atgacattaa cagctgaagt cattgtggac
    10441 gtacctacta tgcagaccga tcagccattc acttatttgg ttccctctga aatggaaaca
    10501 gctttgcaag tcgggatgcg agtagaagtc ccttttggta atggcaatcg tcatgtccaa
    10561 ggatttgtta tggctatcca aaaatcagaa gaatcagcta atccatcttt gaaggcagtt
    10621 attcgtttgc tagaccttgc tccggtggtc aatgaagaat tactttcatt agctgattat
    10681 atgaaaaaaa ttacttacgc attcaaaatc acttgtctac aaacgatgct tccgagtgtg
    10741 atgaaagcag aatatgataa attgatctat cctttggcag acacaccaga agttcaggta
    10801 ttgtttgatc agcgagaagt gatttcctgg aaggaagcag aagaagaagg cagcctttct
    10861 caattgattc gctggcgcca agagcaatta gtagatatca aatatgaagt ccatacacga
    10921 aataaagtaa aaacgattcg tttggtccgt tctttgttaa ctgaaaagca aatcgaagaa
    10981 gaatgggcaa aattacggca aaatgccaag aaacaaaaag aacttctttt gtgtttatct
    11041 gaactgagcc aagaagaacc aattgcttat ttcaaaaata aaggaatcag tactgctgtc
    11101 ttgaatcaag gaaaagaaaa gggctggctg gagtttatag aatcagaacg ttatcgcgac
    11161 ccttataaag accgagtatt tgaccaaacg acggcattgg aactgaatgc agaacaaaaa
    11221 aatgctgtcg aacaaatcat cacagcaggt cagcagcaaa aagatcaggt ctttttgctg
    11281 gagggaatca ctggcagcgg gaaaacagaa gtatatcttc aggcaatcgc tgatgttttg
    11341 tcagaaaata aaacggctat tatgttagtg cctgaaatcg cattgactcc gcaaatggtc
    11401 gagcgattca aaggacgctt cggagaatca gttgcggtct tacatagcgg actttcccaa
    11461 ggtgaaaaat atgatgaatg gcgcaagatt gagcgggaag aagcgcaagt agtagtaggc
    11521 gcacgatctg ctatttttgc cccgctgaaa aatatcgggc tgatcattat cgatgaagaa
    11581 catgaatcaa gttacaaaca agatgagaca ccgcgttatc atgcgagaga tctagcaatt
    11641 tggcgcagta aatatcatca ttgcccaata gttctaggaa gcgcgacgcc ctctttagaa
    11701 tcaagagcac gagctcaaaa aggcgtttat cagttattgc agttaaacca ccgagccaaa
    11761 gcagctgcac agcttccagc tattgagatc gtagatatgc gtgaagagtt tcagaatcat
    11821 cggacctcta ctttttctgc taatttgcag gaaaaaatac aaaatcgatt agacaaaaaa
    11881 gaacaaactg tcctactgct caatcgtcga ggatattcgt cttttgtgat gtgtagggac
    11941 tgtggatttg ttcttccatg tcctaattgc gatatctctc ttacactgca tatggataca
    12001 cgttcaatgc gctgccatta ttgcggacat gaagaaccta ttcccaatcg ctgtcctaat
    12061 tgtggcggca ataaaatccg ctactatgga acaggtactc aaaaagtaga agaggaactc
    12121 cgagaacttt atccgcaagc taggattttg cgtatggatg tagataccac tcgaagaaaa
    12181 ggggcacacg aacagatttt acaaaaattc ggtgcaaaag aagcagatat attattaggg
    12241 acacaaatga ttgctaaagg tttggacttt ccagaagtga cattagttgg ggtgttgaat
    12301 gctgatacct ccctgaattt accggatttt cgttctagtg agcatacatt ccaattgctt
    12361 acccaagttt caggacgagc agggcgagcg gaaaaagcag gcgaagtcgt gatccaaaca
    12421 ttcaatcctc aacattacgc catagaattg gctaaaaaac aaaattatga gcagttttat
    12481 cagcaggaaa tgcatgtacg gcatcgtgga ggctatcctc cttattactt tactgtgaaa
    12541 ataaccgcca gccatcctga agaacaagta gcggcaaaga aaatctttca gattgccaat
    12601 cagctaaaag aagtgttaag ccctcaaagc ttgctattag ggccaacgcc cagcatgatt
    12661 ttgagagtga aaaaccgata ttattatcaa ctaatcatca aatataaaca tgaaccgaat
    12721 cttcctcatg ttttggatga aattttgaat ggaagccaga aagaacagcg acaaggtctt
    12781 tttgtggcaa ttgacaacga accattaaac tttatttaag aaagggccaa attatgcgct
    12841 atcctatatt gatccaccct aatgataaat taaaaagaac tgctcagcca atcgatgtaa
    12901 tcacggacga gacaatcgct ttattagaca atttatatga gacaatgatt gcgaatgacg
    12961 gaatagggat cgctgctcct caagtaggac aaaacaagag aattgctgtg atcgaagtag
    13021 atgaaggcga gaaatttgaa ctgattaacc cagaaatcat cgaagcaaaa ggtgaaagcc
    13081 ttgacgtaga aggttgtctc agtattccgc atgtctatgg aacggtcaag cgagcagatg
    13141 aagtgaccgt gcgttattat gatcgagacg gagaagagat agaagtgacg gcttttggct
    13201 atttagcccg tgccttccag catgaaatcg atcacttaga cggtatcttg ttcattgaga
    13261 aaatgataca gcagatcccg gaagaagaac ttgaagaata tatggaggaa catcttgatg
    13321 actaagctag tatttatggg cacacctgct ttttctgtgc cgattcttga agggctgata
    13381 gaatcaggtt acgagatcca ggcagtggta acacagcccg accgtccagt tgggcggaaa
    13441 cgagtaatta cgccgacacc ggtaaaaaaa gcggctctga agcacggtat ccgtgtgctg
    13501 cagccagaga aaatctccgg ttcgccagaa atggaagaaa tcattgaatt ggtgcctgat
    13561 gtgatcgtca ctgcggcatt tggccaattc ttgcctgaaa agttattgca agtaccaaaa
    13621 ttaggggcaa tcaatgtcca tgcttcttta cttcctaaat atcggggagg tgcgcctgtt
    13681 cattatgcca tcatgaatgg ggagaaagaa actggtgtaa cgatcatgga aatgatcaaa
    13741 aaaatggatg caggcggaat ctatgcgcaa gaaagcatac caatcactaa acaagatgat
    13801 gtagggacga tgtttgaaaa gcttagcctt ttaggaagaa agctattgct agaaacattg
    13861 cccaatattt tggatggaca aaaaccagta cctcaagacg agtcagaagc aactttttcg
    13921 ccaaacatca cacgggaaca agaagcaatc gattggcata aaactgcaga agaaattgac
    13981 aaccaagtac gcggaatgcg tccttggcca attgcattca cgacctatga gggcatacgc
    14041 tggaaattat tatcggttga agtattggca gaaactacag acaaagcacc tggtacgatc
    14101 atccggaaag acaaaaaaaa tctatggatt gcttgcggta aacagaccgt cttagccatc
    14161 aatgaattac agccagctgg taaaggaaaa caggccgtac atgatttcct taacggaagc
    14221 ggtcagcaag ttcagattgg tcagcaggtg aaataatgaa aaaaatccct aaaaaaatga
    14281 aaaaatcagt acgatatatg gcattactga cattagagcg agtgaacaag ggtggcgcct
    14341 actcgaatct tttgttgaat gagatgatca aacaaagcga gctaaatcaa aaagatgtgg
    14401 gtttatttac agaattgatc tatggtacga ttagccatca gcgactattg gaattttacg
    14461 ttgcaccatt gatcgctaaa gcaaaaaaag tggatgactg ggtcaagatg cttctttatc
    14521 tttctgtata ccaattagaa tttttagata aagtccctgc acatgctatt ttaaacgagg
    14581 cggtcgaaat cgcaaaagta aaaggaaatc ctgggacagg gaaatttgta aatggtgtct
    14641 tgaggaatta tcagcgtcaa ggagcaccag atctgcaagg cattgctgat cctatcgaaa
    14701 gactttctgt caaaatcagc ctgcctctat ggatgacgaa acgactggtg gatcaaatcg
    14761 gatacgaaga aactgaaaag ctcggtttat cgctatatca gcctagccat gctagtgccc
    14821 gagtagatac acgccgactt tccagagatc aggccatcac tgttttgcaa gaagaagaga
    14881 tcacagctga aaaaagccaa gtcagtccat acggtatagt agcgaaaaaa gggcatttag
    14941 cttctagctc attgttccac gatggcgtca tgacgatcca agatgaaagt tcgatgcttg
    15001 tagcgccgtc tatgcaaatc gagcctgaac atcatgtctt agatgcctgt gcagcacccg
    15061 gaggtaagac gacgcatatt gcttcttttt tagatccgtc atgcggtgga gaagtggtcg
    15121 ctttagatgt acacgaacac aaagtcaaac tcattgaaga taatgcagca cgtctaggtg
    15181 tctcttctgc tgtctacgct caaaagatgg atgctagaga agtcagtaaa cagttcgaag
    15241 cagagcagtt tgatcggatt ttagtagacg cgccttgttc aggtcttggg ctgcttcggc
    15301 gaaaaccaga tattcgttat aaaaaacagc cggatgaatt agcgaattta ccaaaaatcc
    15361 aattggaaat tttagaaagc tgcgcaccaa ctttaaagtc ttccggtatt ctgacttata
    15421 gcacttgtac gatcttaaaa gaagaaaacc aagacgttgt ttctgctttc ttggaaagac
    15481 accctgattt tgagaggatt gatgttttga ccaatcctat gctacaatca tcaatcgaag
    15541 agaaaatgtt gactttgtat ccgcatcagt tttacacaga tggatttttc atttgttgtt
    15601 tgcgcaaaaa gtaatgaggt gaaaaaatgc agattgaata tcaatcagat gtaggaagac
    15661 gacgtaacac aaatcaagac tatgccagtg tatttaccag ccgttcaggt ctcaagcttg
    15721 ctgtattagc agacgggatg ggtggtcatc gtgccggaga cattgccagt cagatggcgg
    15781 taacgaatct aggcgaagct tgggaaaaac aagaagctgt tgacgatgaa aaaatcgccc
    15841 aatggttcat tcaatcgatt caagaagaaa atatgctgat ttatcagcgt ggacaagaac
    15901 agccggaata taacggtatg ggaacaacaa tcgtagcggc cgctctgtca gatgactggt
    15961 tcacgattgc caatgttggg gatagcagag cttatttgat tcgtgaagat aaaatcatgc
    16021 aattgacgga ggatcattct ttagtcaatg aactagtcaa atcaggagaa atcagcgaag
    16081 aaatggcagt gaaccatcca aggaaaaata ttttaacacg ttctgtcggc atgcctggaa
    16141 cagtcgaagt agatgtttct actcatcttt ggcaactaaa agatcgactg ctgttatgtt
    16201 cagatggctt gacgaacatg ctgtctgaag aagaaatagg aacgatcatc aatcaagaag
    16261 gcccattgtc tcataaagta actgagctga ttgatcgtgc caatgaagca ggcggagcgg
    16321 ataatatcac cgttcttctt atcgaatata aggaggacag tgtatgatcg agttgggcaa
    16381 aaaattaaat ggccgttatc aaatcactgg aaatatcggt agcggtggta tggcaaatgt
    16441 atttttggcg cacgatttaa tcttggatcg tgatgtagca gttaaagtct tacgatttga
    16501 ctttcaaaat gaccagactg cgattcgacg atttcagaga gaagctttag cagcaacaga
    16561 actcgttcat ccaaacattg ttagtgttta cgatgttggt gaagaagaca acatgcagta
    16621 tctggtgatg gaatacgtca aaggaatgga cttgaaacga tatatccaaa cacattatcc
    16681 tgttccttat gaaacagcag taaatattat gcagcagatc ctttctgcta tttctttagc
    16741 tcataaccat caaatcatcc accgagattt gaaaccccaa aacgtactga tcgataatga
    16801 aggtgttgta aaaatcacgg atttcgggat tgctatcgct ttatctgaaa catcgatcac
    16861 gcagacgaat acgatgctag gatctgttca ttatctgtcc cctgagcagg ccagaggaag
    16921 tatggcaacg aagcagtcag atatttatgc tttagggatc atcctttatg agatgttgac
    16981 gggtagcgta ccgtttgatg gtgaatctgc tgtaacaatt gctttaaaac attttcaaga
    17041 tgatcttcca tctataaaag cacttgatcc aaatgtgcca caagcactgg aaaacgtgat
    17101 tttacgggca acggctaaag aacctgcaga ccgttataag tcagccgagg aaatgtctga
    17161 tgatctttct acagtattat caccagctcg tgcaaacgaa gaaaaatggc agcctcatgt
    17221 gatggataat gaaacaaaag ttatcacacc ccttacggaa gatgcaccaa tgcctgattc
    17281 attcaaatct atgcctctgc caaaagacaa gccagaaaac gaaaccttac cagaggagga
    17341 agaagttctg cctgatccta aaaagaaagg acagaaaaaa tggtggctga tcctattagc
    17401 tattcttgct gttttaggaa tcggaacagc tgtattcttt gcttctggcg ggcgaggaga
    17461 agttgttgtt cctgatgttt ctgatctttc tgaagcttct gctagagaaa cactcgcaaa
    17521 agcgaaatta gaagtagcaa caaaaacaga agaaattgca gatgacaaaa tcgaagaagg
    17581 taatgtagtg aagacagacc cggcagcggg aacgacagtc aagcaaaaac gcgaagtcac
    17641 tctgtatatt agtagcggca cgaaaaaaat caaattagat gattacagtg gtaaaagtta
    17701 tgaagatgcc gaagcaagtt tgaaagaatt agggttttct gctgacttga ttcaaaaaga
    17761 agaggaattc agttcagagg tagaaagcgg aatgatcatt tcgcaatctc catcagaaga
    17821 tacagaagtc gatcctaaaa cagacacgat tactttcaca gtcagcaaag gaccagaaac
    17881 agtgactttg agtagtcatg taggagaaaa ctacgaaact gttcgtaatt ggcttgttac
    17941 acaaggattc aatatcaata atattagtgc tacttatgac tatagcgaca ctgtaccatc
    18001 aggttcgatc atcagtcagg atccagcctc gggtgaagtt gtagcagaag aaacttacat
    18061 caattttgtc gtaagccaag gaacagagcc taaattgaat gatatctccg gctatacaaa
    18121 aagcgaagca caaagttatt tagctagtgt cggtgcagaa tatatcgggc atgagacttc
    18181 cgaatattca aacacggtgg ataaagataa agtgattcgt acagtaccag gggcaggaac
    18241 gacaattacc aaaggaatgg ttgtgaacgt aatttactct aaaggaccag atccagcgtc
    18301 ttctagttct gaaaaagact cttcagacag ttctagcagc tcaagtagct ccagtagcac
    18361 aagcaactct tcaaaagaag aatcttccag ctccacaagt tcatcagcta gcacctcaga
    18421 aagttcaaaa taagtgctga ataaaatata tcagaaggga atgcgacatt gatttgttgc
    18481 attctctttt ttgtgtttct atctcttttg atcaattttc aagccatata ctttgagaag
    18541 tttgcatgga ataacaccta ctttcctctt cgtatggtaa aataaacaaa aacggaggtg
    18601 gcacttatca aaggacaaat cagaaaagca atcagcggtt tttactatgt atacgcaaac
    18661 ggagagacct atcaaacaag aggaagagga aatttccgga atcggaaaat cacgccgctt
    18721 gtcggagatc aggtgatctt tgagagtgaa aatcaaacag acggctatct tctggaaatc
    18781 ttgccgagaa aaaatcaatt agttcgccca ccagtcacaa atgttgatct tggaatcatt
    18841 gtcacaagta tcgtagagcc tgcattttct tataatctac ttgaccgctt tctagttacc
    18901 ttggaatacg agtcgatcga accgattatt tttttgacaa agaccgactt gtcagacgac
    18961 ccagaaaaaa tcgaagagat caagcaaacc tatactaaaa taggctatcc ggtgattgta
    19021 cccaatcata cgggtgatac gacagaactg atccgttact tcccagaacg gctatcggtc
    19081 tttatgggtc agtctggagc aggaaaatcg acgctactga atcaaatatc accggaactg
    19141 aatttagcaa caggagagat ttctgatgca cttggaagag gacggcatac aacacgtcat
    19201 gtggaactga ttcctatcta tggcgggtta gtagcagata caccaggatt tagctcgatt
    19261 gactttttga caattgaagc aggagaactg ccgagacaat ttcctgaatt tgtagaagct
    19321 tctgtccatt gcaaatttag agagtgcatg catgctaaag aacctggctg cgaagtaaaa
    19381 catagagtag aaacaggtgg tatcgcacag acacgttacg acaattattt gcaattctta
    19441 caagaaatcg aaaaaagaaa accaatgtat gcaaaaaaag aaggtaaacg aaaataggag
    19501 gaaacaagaa tgaagatagc accatccatt ttgagtgcag attttgccaa tttacaaaga
    19561 gacattgagt tagtggaaaa aggaggcgca gattacatcc acgtagatgt aatggatggc
    19621 cgatttgtcc caaatatcac attagggcca aatattgttc aggcaatccg tcctgttact
    19681 aaattaccgt tagacgtcca tctgatgatc gttgatccag aaaactacat tccagcattt
    19741 gcaaaagctg gagcagatat cattactgtc cacgtcgaag caactcctca tattcatcgg
    19801 gcacttcaaa tgacaaaaag cttaggagtt aaagctggag tagttatcaa tccaggaaca
    19861 ccagtctcca tgatcaaaca tgttttgcct ttggctgatc aagtactagt catgactgtc
    19921 aatcctgggt tcggcggaca aagctttatt gaagaaacag tagaaaaaat tacggaactc
    19981 gctgatctac gtagagagaa caattggact tatgcaatcg aagtagatgg cggaatcgtt
    20041 ccagaaacag caaaaatctg taaagatgca ggggcggatg tcttcgttgc aggatcatat
    20101 atctatggag ccgaaaatcc agtcggacaa atcgaacgat taaaagaggc attagactaa
    20161 atgaaaattt tattagctgc tggaggtaat ccgcaaaact ggccgcagtc tctgctgtcc
    20221 aaatacgatg cctatgtcgg gatagaccgt ggtgcgctgt acctgcttca agcaggctac
    20281 ccagtagatc tggcagtggg tgattttgat tcattgtcga acgaagagca gaaggaagta
    20341 tttgcaaaag ccaaagagta tacccgttcc cctgcagaaa aagacgatac agatacacag
    20401 ttaggtatct taaaaacatt tgaacgttac ccgcaagcgg aagtcacttt gattggagct
    20461 acgggcggaa gattggatca tcttttagcc aatctctggt taggtatgga acctcgcttc
    20521 aaaccctttg cccatcagct tattatcaga gattgtcaaa attatttgtc ttattatttg
    20581 cctggagagc atatagtaag acagatacca gatatgaagt atttagctta ttgctgcttg
    20641 actcccgtct cgaatcttac attaaaaggc agcaagtatg tattggaaaa taaagaagta
    20701 gaatatccga catcttatgc aagtaacgaa tttctttcag ataccgcaga attttctttt
    20761 tcagatggta tgattgcagt tattcaaagc aaagattgat ccataaacaa ataaaaaaat
    20821 ttgtctttca ggctaaaaat gacagaatct attatttgct aaaaaaaagc ttaagaggag
    20881 caacttacct tttgtttcac ggcatttagt gataaaattg aacacaagag tctaattaat
    20941 tcgaggtgtc tttgtgaaga agttcaatcc taataaaaat attattatta ccttggttat
    21001 tgttatcatc gtggtgacga tactaagtgt gaccgctgcg cagcgagcga atgaaggaaa
    21061 gcccaatttc ttccaatcca tcgttaatga cagtgttgca atggttgata aaacaatctc
    21121 tgccccagta cggtgggtac gaaatggagt agattctgtc cataatcttt tttcaactta
    21181 tgccgaaaat gagcggctaa aagaaaaaat cgatagctat gatgacgttt cacagcaaaa
    21241 caagaattac gagagagaaa tcgaagcgtt gaaaaaagaa cttgagttga atcagacgct
    21301 gactagttac gaaaaagtaa cggcaaatgt cattactcgt tcgcctgaca gttggcagga
    21361 catgctgatc gtagacaagg gttctaaaga cggtatcgaa gcaaatatgg cagtcatgtc
    21421 gcaaaaagga ttgattggcc gagtgatcga ggtcaatacg gcttcgtcta aaatcgaatt
    21481 actgtcatcc tctaatgaaa gctccaatca ttttccagta cgggtatctt cggctaatgg
    21541 cgaagcgttt ggtttgctta aaaactatga tgaaaagctc catgccttag tggtgaccca
    21601 attaactggt gatacggata tcaaagaagg ggatgttgtc cagacatccg gtcttggagg
    21661 gaattctcca gctaacttgc cgatcggtac ggttattaaa acgaaaccag atagttatgg
    21721 gctggatcgg gaagtttatg tgaaacctta tgcagaaatg tatgacgtgt cagttgtgac
    21781 gattgtccaa cggctagcgg aggattagtc catgttgaag aaagaaacaa tgaagtacta
    21841 tctgccaatc gttttgttct ttttgatgtt gatagatggt catttaacaa gaatgctagg
    21901 ggagtggtcg aaaggcacct atatgtcaaa tgcccacttt ctgatattgg cattattatg
    21961 ttgcagtatg gcgtttgaaa aacgttattt actgattacc acgattgttc tcggggctat
    22021 ctatgacgct tactatattg gcgttatcgg tatctatgca gtagctctcc ctttaattgt
    22081 atggttgatg tatgtaatga aagacgttat ccatgtcaac atctttactg aatttttcag
    22141 tatgatcatc tttgtcacgg gttatgaatt gtttacgatg gtggtccagt tgatttttaa
    22201 attagcagta gtaaataaca cgtattttat tacaaggttt ttaggaccta cactgctgtt
    22261 gaacatgatt atatttgtat tattcatttt tccctttaag aaattattca gtgatgaata
    22321 atttcttttt ttatttttag gtcgaaagcg aaaaagaatc gttgttatct taagaaatgc
    22381 tagacttaac gcggcttttt catgatcatt ttctgaaacg aacagttttg tcatttttaa
    22441 aagttgtttt gaaacatttt tgtaacataa ctattatatc gttgacacac tgctatggta
    22501 caatttttat atcgttaaga aagacttaaa tatatttaga tatttattaa attaagccag
    22561 tcggaggaat gaagagtgaa aaagagttta atatcagcag taatggtatg ttcaatgacc
    22621 ttgactgccg tagcatcacc gatcgctgcc gcggcggacg attttgattc tcagatacaa
    22681 cagcaagatc aaaagattgc agacttgaaa aatcaacaag cagatgcaca atcacaaatc
    22741 gatgccttag aaagccaagt ttctgaaatc aacacacaag cacaagacct attagcaaaa
    22801 caagatacat tgcgtcaaga atctgcacaa ttagtaaaag acattgcaga tttacaagaa
    22861 cgtattgaaa aacgtgaaga tacaatccaa aaacaagcac gcgaagcaca agtaagcaac
    22921 acaagctcaa actacatcga tgctgtattg aatgctgatt cattagcaga tgctattgga
    22981 cgcgttcaag caatgacaac gatggtaaaa gcaaacaacg acttgatgga acaacaaaaa
    23041 caagataaaa aagctgtaga agacaaaaaa gctgaaaacg atgctaaact aaaagaatta
    23101 gctgaaaacc aagctgcttt ggaatctcaa aaaggcgatc ttttatctaa acaagctgat
    23161 ttgaacgtac tgaaaacttc attagctgct gaacaagcaa ctgctgaaga caagaaagct
    23221 gacttgaacc gtcaaaaagc agaagctgaa gcagagcaag ctcgtattcg tgaacaacaa
    23281 cgtctagcag aacaagctcg tcaacaagct gcacaagaaa aagctgaaaa agaagcacgc
    23341 gaacaagcag aggctgaagc acaagctact caagcttcat ctactgcaca aagctcagca
    23401 acagaagaat catcagctac acaatcttca atgactgaag aatcatcatc agctacacaa
    23461 tcttcagcaa cagaagaatc aacaacacct gaatcttcaa cagaagaatc aacagcgcct
    23521 gaatcttcag caacagaaga atcaacaaca gcgcctgaat cttcagcaac agaagaatca
    23581 acaacagtgc ctgaatcttc agcaacagaa gaatcaacaa cagtgcctga atcttcaaca
    23641 acagaagaat caacaacacc tgcgcctaca acaccatcaa cagatcaaag tgttgataca
    23701 ggaaacggca caggaagtag tactccggct ccaacgccaa caccaacacc tgaacaacca
    23761 aaacctgtaa cacctgctcc agctccttct ggaagtgtga atggtgcagc aatcgtagca
    23821 gaagcttaca aatatattgg tactccatat gtttggggcg gtaaagatcc aagtggcttt
    23881 gactgctcag ggttcacacg ctatgtttac atgcaagtaa ctggtcgtga catcggtggt
    23941 tggacagttc ctcaagaatc agctggaaca aaaatttcag tttctcaagc aaaagctgga
    24001 gacttgttat tctgggggtc acaaggcgga acttaccacg tagcgattgc tttaggcgga
    24061 ggacaatata tccatgctcc tcaaccaggc gaaagcgtaa aagttggttc agttcaatgg
    24121 tttgcccctg actttgctgt cagcatgtaa gaatga
//
LOCUS       DAYYCW010000037        23953 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000037 DAYYCW010000000
VERSION     DAYYCW010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.037"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1273..1938)
                     /locus_tag="ACHAYA_001332"
     CDS             complement(1273..1938)
                     /locus_tag="ACHAYA_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8605140.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="ACHAYA_001333"
     CDS             2064..4100
                     /locus_tag="ACHAYA_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFM8605141.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="ACHAYA_001334"
     CDS             4104..4742
                     /locus_tag="ACHAYA_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8605142.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="ACHAYA_001335"
     CDS             4770..5198
                     /locus_tag="ACHAYA_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605143.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="ACHAYA_001336"
     CDS             5655..6566
                     /locus_tag="ACHAYA_001336"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8605144.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6783..7220
                     /locus_tag="ACHAYA_001337"
     CDS             6783..7220
                     /locus_tag="ACHAYA_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605145.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7315..8199
                     /locus_tag="ACHAYA_001338"
     CDS             7315..8199
                     /locus_tag="ACHAYA_001338"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8605146.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8423..11590
                     /locus_tag="ACHAYA_001339"
     CDS             8423..11590
                     /locus_tag="ACHAYA_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM8605147.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11658..12155
                     /locus_tag="ACHAYA_001340"
     CDS             11658..12155
                     /locus_tag="ACHAYA_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFM8605148.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            12174..12638
                     /locus_tag="ACHAYA_001341"
                     /pseudo
     CDS             12174..12638
                     /locus_tag="ACHAYA_001341"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            12635..13393
                     /locus_tag="ACHAYA_001342"
     CDS             12635..13393
                     /locus_tag="ACHAYA_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8605149.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(13479..14219)
                     /locus_tag="ACHAYA_001343"
     CDS             complement(13479..14219)
                     /locus_tag="ACHAYA_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8605150.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            14280..15533
                     /locus_tag="ACHAYA_001344"
     CDS             14280..15533
                     /locus_tag="ACHAYA_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8605151.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            15855..16082
                     /locus_tag="ACHAYA_001345"
     CDS             15855..16082
                     /locus_tag="ACHAYA_001345"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8605152.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            16205..16531
                     /locus_tag="ACHAYA_001346"
     CDS             16205..16531
                     /locus_tag="ACHAYA_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8605153.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            16599..17312
                     /locus_tag="ACHAYA_001347"
     CDS             16599..17312
                     /locus_tag="ACHAYA_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8605154.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            17622..18472
                     /gene="licT"
                     /locus_tag="ACHAYA_001348"
                     /pseudo
     CDS             17622..18472
                     /gene="licT"
                     /locus_tag="ACHAYA_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            18593..20458
                     /locus_tag="ACHAYA_001349"
     CDS             18593..20458
                     /locus_tag="ACHAYA_001349"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8605155.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            20472..21915
                     /locus_tag="ACHAYA_001350"
                     /pseudo
     CDS             20472..21915
                     /locus_tag="ACHAYA_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            22023..22724
                     /locus_tag="ACHAYA_001351"
     CDS             22023..22724
                     /locus_tag="ACHAYA_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8605156.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            22850..23362
                     /locus_tag="ACHAYA_001352"
                     /pseudo
     CDS             22850..23362
                     /locus_tag="ACHAYA_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(23760..>23953)
                     /locus_tag="ACHAYA_001353"
     CDS             complement(23760..>23953)
                     /locus_tag="ACHAYA_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605157.1"
                     /translation="QILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNLIIFVHQ
                     LLLKIFLLTINSWICKNIT"
BASE COUNT         8064 a         3912 c         4938 g         7039 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaactg ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta
     6661 cttgatttag ttctttttat tttaaaaatt ttttttactt agaatccagc gaccctagat
     6721 atttttctgt ataatcttaa ataaagaaaa caatatcaaa aaatgcacgt agaagtagat
     6781 aaatggagtg gttttgcaaa tttcttttat ttctagaagt tattcacgta tacggatttg
     6841 tttttctcac aaataaaaag gcaggaagta caatggatca tttgtatata aaatatattc
     6901 atcaattttc cgttttggtg ttaggcacac taattatagc ggggattgtc gcatttatta
     6961 ttcaaggccg tgacatatta acagtacatc aagagttttt cagttatgca tttggtattc
     7021 ctctgatcgc tataaggtct gataacaaaa agtggaagaa acgctattac attctcttgc
     7081 tatttttgtg tttttatctg ccacctaaga tttttacttt attaggtata caatgcctat
     7141 cattttcttt tatttcctat atcattgttt taattttcct cagtttcata gtatggatgt
     7201 tttatcttgt caaaaaataa atgaatgttt catgaacagt acttcatcac agcctaattt
     7261 ttttctttat gctatcgtta tattaaagaa atcaatcaga agggaaagaa agtgatgaaa
     7321 aaagttttat tagtggtaaa ccccagttca ggcggtgaac aagcaaagga atttgagcaa
     7381 ttagcgatag caaaattgga atcggtgttt gatgaagtgg tcgtcttgca tacaaaaaaa
     7441 gcaggggatg caaaaaactt tactcgcgaa gcggctacgg aagggtatca tagtgtgttt
     7501 gtcatgggcg gagatggaac agtcaacgaa ggaatcagtg gaatcgctga acaagaacat
     7561 cggccaaatt tcggattttt cccattgggt acggtgaatg acttggctcg agcattaggg
     7621 ataccgctag aacctgagga agcgatcaat catttttcga ttgaatcagt caagccattg
     7681 gatattggaa aaataaatga cgactatttt atgaatgtcg tcgcaattgg ctctatccct
     7741 gaatcaatca atgatgtaga tgcggagaag aagacgaaat ttggaaaact agcttatttc
     7801 atgtcaggaa tcaaacagtt agcttccaca caatcctact cgtttcacgt agaagttgat
     7861 ggaaagaagg aagaaattga gagcagtacc ttgctgatag gactaactaa ctcagtcggc
     7921 ggttttgaaa cattacttcc aaatgcaaaa gtggatgatg gtaagctcca tttggtttat
     7981 ttaaaagatt cttctttgat cgatacgctt aaaacattgc cagacctatt aaaaggagta
     8041 gatgaatcta cgaaaaatct agcctacctt acttgtgagc aattagttgt gtcattagct
     8101 gatgaggaag aattgacgac gaatgttgat ggagatgaag gggataaatt acctgtgaca
     8161 atcaaagtat tgccctcaca tttacaggtt tattgttaaa atggaggttg tgaccgagga
     8221 gttggcggga gaacaccgct tgttcgggat aagggactgt gacaagactt ttgtcacagc
     8281 cttttttttg tacaaaaaag aaatttacac ctaatagaaa aatttttcca taaaaataca
     8341 tattttaaat atgaaagtac aattggaaat agattaaaac gataaaataa aattgtcaaa
     8401 acgtcagaga ggatggtgga gaatgaatca atgtgtaaaa aagctgattg cgtcgatttt
     8461 gtttgtcttc cctctcttga tggtgacatc cgtttttgcc gatgtagaga aaacaaacga
     8521 atcaacgcaa tcagagaatc aaagttttga caagactact ttttcagaag aatcgattac
     8581 ttctgaaact acagaatctc aaagtgaggc ttctactaat aaaaggcaag aagaaaataa
     8641 gacaagtgag acctcagaag tagctgaaca aacgtctgat agcagtgaag cggcactggc
     8701 aagagctgtt tcagcggatg ggtacagcga agctgccaca acagaagaac tggcgcagct
     8761 tctcgcagac gaatcagtaa ctaagatccg actgattcag cctttgactt tagacaggga
     8821 attagagatc aaacgagata tcgttattga ttttggtgga ttcgctcaca atttcggcac
     8881 acaccatatt tatatcaatg agatacctca gtcgatcgag ttccagaatt ttaaaggaac
     8941 agctgcacat ccaggtggat tgacaccaaa tatcgatgga aatgccatta tcattgctta
     9001 tatgaccgat ttttggggaa gcagatatca ttttacggga gaaataaaat ttaccgggac
     9061 acttgatttg tatgatgggt ccaaattagg actgatttac gctcctcgtg caactgtcac
     9121 attagatggt gtttccggtg tgttggatgt ccaaccagtg aaggaaggac tgaatgccgc
     9181 tccgggtaaa gcctattttt gtagaagcta tcaattgaat gtgatcaatg gtagtcaact
     9241 ttatggaccg tatctaggga aattctacgg ttttctagat tcaggagatg agactggtag
     9301 tggaaaaaca gcgccaggaa tccatatctt aagcggttca aaggtctctc ttgattatga
     9361 tcgggcagat ggcgttagcg atgaaggaga ggcagtcgat acgctacctg gtaatgtcat
     9421 gtttgaagtc agtggttcgg gaagcgaatt ttcggtaaat acaaagatca atattaccaa
     9481 tgacaacaat cgcggaatca tccaaatgag aggttcagga tcgaaagtca ctgtatcgaa
     9541 tggtgggaaa atcaccatca atacagcaac tacgggcgga tttcgcttgc aaggagattc
     9601 tagtcaaatc catgtatcaa gtggtggaca aatctcggtc aaaatggctg gagatgacga
     9661 tcaacccggg aacaatggga tgcgttttgt aggaaaaggc ctttctttga tcgtagaagg
     9721 agccggatca gttatcgaca tcgataagca gagtgggcgt tccagcgcag ttcgttttga
     9781 gaatggtacg caaaagctga cagtaaccaa tggaggctca ttgagggtaa gaaatacagg
     9841 tgatggcaaa tcttacgtga acagactcaa tcaaggaaat caagcgatcc aattttatga
     9901 cgcttctggt tttctagaaa gtccaggttc agctgatttt acagtcactg gagaaaaatc
     9961 aaatatcgat atacgcgcag attccggagc aactgtagat acaactggag atattgattt
    10021 aaattttacg gcaggtgaaa aaacttattt agtcatggtc gggaaaacag gggaagataa
    10081 gaccggcatc atcagtgggg atgtgttgaa tgtggactta gaatctccta cctattttga
    10141 tttccaaaac aaacggattg gaagcgagac caatggcata ggcggctggg tattccaagg
    10201 aaatggagat tcttctttaa agatcgccca ttcagaaatc gctttatggc gaaaaacagg
    10261 cgcaacattc gataactttg aaaaagaccc ggattatttt tctgatatga cagacttatc
    10321 gatcaatggg accgacttag gcaatttcgt atcaagccct agtgcaggtt taacaaatga
    10381 atttgctcag acaggtgccg gaatgaaaaa ttataatcga atcagtgcca ataatcagct
    10441 cccgatgatt gaatccttaa gagtgccaac gaatgcagat aaaaaaatct atgggcatgt
    10501 cgttgttccc gaaggtgtcg aggcaaagcc aagagatgct tggacaggcg aagtagaagt
    10561 gactttacgt attacttacg cagatacgac gaaatcatct gtagaaatta ctgctctcac
    10621 gcaaggacaa acagatgaaa atgaaggggc aggatataac ccatggggag aaggcgaaca
    10681 aggcggcttg ttcgaagttc aagtacccaa tgatgagaga ttaaaaacgg aggatcaagt
    10741 gcaagttgtt gctgctaaaa aagtaagagg tggccaagaa gtgacagaac tggactctgc
    10801 caaaacgaca gtcgatgtga ttcctcctga acctgctaat ttaagtacca cattgatcaa
    10861 tgcagcttct cgcacgatca caggtacagg tgaagcagga gccttggttt cattgaaaaa
    10921 aaatcaagct gttttgtcag aaacaactat tgatgataca ggggaatttg ttctttctat
    10981 cccagaaaat caactttttg caggagatgt attagaaatt gtattaaacg accaagcagg
    11041 agaagctact gctaagggtg tgcagaacaa gccaagtacc aacgatgaaa ttgggaatca
    11101 caatccaagt gatacgccag ttgtttatca tgatgccccg ccttttgcac ctgctacgaa
    11161 gatcgtagta gaaggtggtc tctcatttct tgccccttcc aaactcgatt ttggtcagat
    11221 caaagcaact ggtttgacac aagaagcatt tggccaaatg gatatacagg aaaaattatg
    11281 gatttatgat cagcgagaaa cgaaaaatcc gtggatctta acgataaaat tatctcagcc
    11341 tttcacaaat gatgcaggat tttctatgca ggatgtccta tatttcaaga aaggagagga
    11401 gtatcaatta gtgaatgaag aagaactgga aattgtcaga ggacaaaatt caacgaatgt
    11461 gcaagcagat tattcagaat atttaaataa cggtcgggcg tttaaacttg tcttaaccaa
    11521 agaaaatcag atagctggta attactcagc agagataaca tggacattag cagatgcgcc
    11581 gacagaataa agatcaagga ggatgattga tgaatcgaat atggaataaa agtcatcgaa
    11641 acaactgctc tttacttatg atccttctcc ttggattcat cctttggcag acacctgtcc
    11701 atgcctccga attcaatttt gcggtagatt ttgaattacc ggctaatcag attgataaga
    11761 acaaaacata tataaatgta aggatggagc ctgggaaaac acaggaaatc ccctacacgg
    11821 tctttaaccg aacagataaa tcagtaacag ttgagacatt gatccactcc gctacgacga
    11881 attcaaacgg tgtgatcgaa tatggagaat caaaaagtaa atttgatgaa acattaagtt
    11941 atgatatgaa aaacttagca aaaacagtgg gagaaataac tgttcctcct caaagtgcgg
    12001 tcactgagac actttcactg aaagcgccag aaaaagagct ggctggtgtg gtcgcaggag
    12061 ggatcacgtt tcaagaaaaa aaagagaacg tttctaacaa aagtacagtt ggtgtcgtca
    12121 atcagttttc tcaagtgaaa gcaatcttga tttgaaatag cgaaaaagag tcttccccag
    12181 atttgaaatt aaaaaaagct tatgcggatc aaagcgatgg aagaaatgtg attaaggcga
    12241 atcttcaaaa tccaaatgct tcttatatgt tcggcttgac tacatcggtt gaaatctaca
    12301 aaaaagaaac aaaagagcct tacctttcaa taaaaaaaga aaaaatagat gtagcaccca
    12361 atacaaattt tgatttactt gtcccattag aaggaaaaaa attagctccg ggacattatc
    12421 aagcggttgt gaagggtgct tggaaagaaa aacgctggaa tctccatacc gattttgaaa
    12481 taactgatga tgcagcggac caattgaatg aaaaggatat tgatttaaaa aatcagccag
    12541 aaacatttga ctggcgatgg ctgatacttg ttggagtcat tcttattggc ttatttgctg
    12601 gattatttat tttaaaagaa agaaggaaaa agaaatgaaa aaaacaattg cattaagtac
    12661 cgttctagct ggaagtctat tattatgtac acaaagtgtt tttgctgaga ataagagctc
    12721 agccactaca gaaggaacga tttctgtgac caaaccagat gctgcaaatc ctgaagacgt
    12781 tgttccagtt cctcctgttg atccaaatga tcctgatact ccttttacac ctgacgaacc
    12841 aaatcaggga acatccggat tactgacgat tgaccaagct ccagatcttg attttggaac
    12901 aatcaagtta ggtacagcaa aaacagtata tgctgcatta caaagcggaa cggacagtac
    12961 tggagcacaa acacaagtac agaatttcgt acaagtaacg gataaaagcg gaaattatgc
    13021 cggttggaaa ttatcagtca aacgaacaga atttattggt acaacatctg atacgaataa
    13081 actaactggt tcaaaggtga aattcaaaaa cgccgtgatt cgaaatagcg caactaatag
    13141 cagtggaatg ccatctaccg tttctggaac tggcgctgaa ggaatcgaaa tcccagcaga
    13201 tcaggcagta gatctagtaa ctgcaggagc aaatgaaggg attggcacat ggatttattc
    13261 attaggagag aatgtggaac aagggagaca atcagtggaa ttatttgttc ctgtatccaa
    13321 ttatgctgta gacaactacg tttcaacatt cacatggtcg ttaacagatg ctcctacaga
    13381 agaggaagcg taaaatcata tcaacgattt cagggagtgg agccgattca aggcccactc
    13441 cctgaaatct gctatgctta cgtctcagct cttattcatc aactcgaagc agttttttgg
    13501 aagtcatgga aacgttcctt tgtcttttca aagatctttc gaccaacgag taaggagtca
    13561 ccgttgaaaa attctaacac ataatgagca gaatcaattt tctgcaattg cgataagttg
    13621 atacagtaag attttaaaag taaaaagtaa gcatactttt ctaactctgt cttcaattgt
    13681 tttaaagaag tattcactaa tttatcttct gtagttgttt tgaccataac ttggtttttc
    13741 aaacctttga tactttcaat atataaaatt tcccgaggat acaggacgag acttccacct
    13801 ttttgatcaa taaagatttt atcgacattt acgatctgct gttctttcca attgatctgt
    13861 tctagcactt gacgaatttg ttcagataaa ttcctgtctt ttctgatata tccaatcgga
    13921 aggattttcc gattgatgat ctctactgca aaattcgtat aagcggtgaa aaaaacaata
    13981 ttactatgtg catttttttt acgtatttgt tcggcgacat caatacctgt gagtgattgc
    14041 ttcaagtcga tatcgataaa gaaaacatcg tgattatcaa tagtgcccaa ctggatcaac
    14101 tgattgattt gagagataga agtgagctgc acgatattct cgatatgatg attgaccaat
    14161 tctctgatga tccattcgct gtgaaactgg ttatcttcta tgataaaata ctttttcata
    14221 tgcaggctcc tattaaaaaa atatgtgtac attttgaaac aaaacaatgg aggaataaga
    14281 tggtcgcaac agtgacactt tatttgacct ttactttgat tttagattta ggtatctatt
    14341 tattcttttc gaaacaactg ctagtaaaaa acagctttgg ttgtatagct ttgatggtgg
    14401 tgacactttt acttcctatc cctaaagtga gctttcctta cctagttggc ttattattga
    14461 tgtttctttt ttattttact acgaaaaacc tgtttatcag ctgtttgatc agtaatcttt
    14521 tttttgctat cactggactc agcttttttc tggtttttga tctgatggat tatttaggaa
    14581 agagacatct tggaacagca agtttttttg cactacttat cgcatttatt ttctatctat
    14641 tagtaaaaag tatagacaaa cagacgagat ttctcaactt gctttttact tatccaactg
    14701 aacactattt tggaggtatt tgtattttat taagctgggg cgtgatttac ttggtattgg
    14761 gaagtgttca cttccgctca tctaattatc tgcttctatc tcttttgtcg atagtcatca
    14821 gttttttttc tttgtttttt tccattatgt tgatcagttt ccaagtaaaa gaaagacaac
    14881 atcaggaatc gcttcaactg tatcaaataa atgaagaata ttaccatcat ctagaggaat
    14941 tcaaacatga ttataaaagt ctactgtttt cattaaaaat tactcttcag gaaaataaaa
    15001 aagaaacgtt ggaattttta gaaatattag aagatcattc tgcagctatt tttgaaaaac
    15061 cgcaaataca acagctaaca aatattgtat ccccagctgt caggggtgtc tttttaaaat
    15121 ttctagaaaa agcagaatcg gagaaaatcc cgatccaagt agttattcct gtcgaagtga
    15181 aaacaatccc gattgatctg gttgttttta ttcgctgttt atctattttt atttctaacg
    15241 cattcgatca tcgtgtagcc gatcagagtc cgattacgat caatctgctg gaagaaaaag
    15301 agggatcacg ttttagtata gcgaacaagg caaatccaac gaatttgacg ctatcggaga
    15361 tggtcaaaag aggaaaaacc tctaaaaaaa atggcgggtt aggtctatac agtgcgaaaa
    15421 aaatgttgga tgtttacgaa aatgcagaac taaaaatcga attttcaaaa cagaatcact
    15481 atttttttgt agaaatgtat ctggcatcta gaaagagcaa atcagcctac tgacttactg
    15541 tcaaaggata agtttgagga aagtctacac ggagatagag acaacaacac agcatattca
    15601 aaagaaacag aagcgtgatg accgttgtca cccttctgtt tcttttgcta tgtagttaaa
    15661 caaagtagtt gacacttccg ctacttagta ttagtacgaa agtatacttc atgatactaa
    15721 gtagataaga agtgaaaagc ggaacccacg atcgtcaaaa tgctaactgc tttgcttaca
    15781 ccagataaag ggaagagcaa tatcgcttat tttaggcgtg gagaagcaag caaatcccta
    15841 cgatcacaaa aaaaatgctg acccagcagt taagagagtt agaacaagat gaaagtatcc
    15901 atcgaaaagc ctataaccaa gtcccgccta aagtagagta ctcgctcacc gaagaaggaa
    15961 aatcattaag agagattcta gtagcgttgt ctgtttggga agaagaacgt atcgaaaaac
    16021 aaaaagcctc aagggaagaa gacacgctac tgcatacaca tggtggctac ctaaattatt
    16081 gattttctat gacatttatt ttaaataaaa gaaaacagga gtttttttaa aagcatcatc
    16141 tattttttat gtaaaaaaat ggtatgatta atcaagtaag aagtgattgg aagaaggcaa
    16201 gcaaatgaat agtatggaaa aagtcaacca gtttagagat gaacgtaatt ggcgacaatt
    16261 tcataacgaa aaagatttgg ctatctctat ttctttggaa gcatctgaat tgttagaact
    16321 ttttcaatgg aaaacgccgg aagaagtaaa agaaacacaa ttagaacgaa tcaaagaaga
    16381 actggcagat gtattgatct attcatacat gatggcagac aaccttcacc tggatctaga
    16441 tgagatcatt gaagaaaaat tgatcaaaaa taatttgaaa tatcctgtag aaaaaagcgc
    16501 aggtgtacga aaaaaataca cagagttgta gaaatgtgtc attcggctgt tggaaagagc
    16561 aaaagaaaaa cagtaaaatg agtctggagg gagagaaaat gaaaaaaaaa ggaaaagcaa
    16621 aaagatggct agtcaatatc ctcttgttcc tcttgttatt agtaggacta gctttgattt
    16681 tcaatgaaca gatcaaggat tattttgtca gagagacagg agataaatat gctatagcga
    16741 atgtaacaaa agaagatctg aaaaagaata acgataaaga tgtcagtttt gactttgatg
    16801 cggtagaacc aatgacgaca gaaggggtca tgaggtcgca aatgagagga acagatctac
    16861 cagtgattgc tagtatcgca gtcccttcag tttcaatcaa tttaccggtt ttcaaaggct
    16921 tggataatac gtcactactt tacggagcag gaacattatc tccagatcaa gaaatgggaa
    16981 aaggaaatta tgccttagca agccatcgtg caacgaaccc tgaattgctg tttacaccgc
    17041 tagaaaattt agagatgggt gccaagatct atttgacaga tttagaaaat gtgtatacat
    17101 acaaaacatt ttttaaagaa aaagttgctc ctacagatac ccaattactg aatgaagtcg
    17161 agggaaaaga aattgtcaca ctaattactt gtggcgacat ggacgccgtc acgcgactag
    17221 ttgtacaagg agaactggaa agtgttacat cgataaaaga tgcgacagat gatatgagat
    17281 ctgctttcaa tctagagaca aaaacatttt aggatactgg gacataagtc gttacagatt
    17341 aaaaacagcc aaaggatatg aatcgataag gaaaaaccaa tttgctgaaa aaaagagaaa
    17401 cgcaaagagg tgcatctctg atttggttgt tttctagata gacttgtgtt ccagtttttt
    17461 tattttttat atgaaaaggc ttgcataaat aaaaattcga tggtatgatg attttgaata
    17521 agaaatggat tgttactgta cggcaggcaa aaccagaatt cagctaagaa acagtaggcg
    17581 aattctaggt ttttttattt ttcttgagag ggcgaagcaa aatgaaaatt gataaaattt
    17641 tgaataacaa tgtcgtcatt tcgaaaaatg gattcgggga agaagtcgtt tgtatgggaa
    17701 aaggactagc ttttcaaaaa aagattgggg acgagatttc gccagaagcg gtccaaaaag
    17761 aatttgttta agagattctt ttgctaaaaa ccaattccag cagctgatgg ctgatattcc
    17821 ggcagaggag atcgaatggg tcaaacaagt agtcgaactg gctgaagata aactaaaagt
    17881 tgaattttcg cctaatattt atctgacatt gaccgatcat cttcattatg cgatcacacg
    17941 agcaaaagaa aatatccaac tgcctaatcc attattattt gaaacaaaaa aattttatcc
    18001 gaaagaatat caagcagcga aagaggctct tttgttcgtg aaacaaaaga cgggtgtgga
    18061 actttctgag gatgaagccg gttttattgc ctttcatttt gtgaatagcc aacaaggaaa
    18121 cgaagacatg caagtgacga tgaccgctac cacaatggta agagatatac tgagtatcat
    18181 cagtaaattc ttcggcatcg tgttcgatga agaatctttg aattatcagc gtatcattac
    18241 tcatctgcag ttttttgccc agcgttatct caaaggggaa ggatctgacg agcaagatga
    18301 atttctctac gcattagttc aaggaaagta tccaaaagcg tttcattgtg tcgaacggat
    18361 caatgagtat cttcgacaaa cgcaaaaaca ggaaatgggg atcgcagagc agatttattt
    18421 aacgattcat attcaaagag tagtaagtga gaaaaaaagc atccaatcat aaggattgtt
    18481 actgtatggc aggcaaaacc tggattgaaa aaataggttc acgaatgttt gtgactgttt
    18541 ttttcaatct gggtttttat tttttaaata aaaaagaaag aaggaaaaaa caatggataa
    18601 tcaagcagtt ggtcgtcgtg tatgggaagc ggtaggagga caaaagaatg tcaaaagttt
    18661 agtacattgt gctacgcggt tgcgattcag attaaaagat gaatctttag cagatacgca
    18721 aaagttaaaa gaagacccgg acgtgatcca agtcgtacaa agtggcggtc agtatcaagt
    18781 agtgattggc agtaatgtag cagatgttta tcaatcgatc gtggatgagg aaggtcttgc
    18841 agtttcggag gatggaaaag aagagtccaa aaatattttg aatcgtctga tcgatattat
    18901 ctctagtata tttacacctt ttctaggggc gatggcagcc gccggggtat tgaaaggatt
    18961 cttatcattg gcaacggtga tgggctggct gacagctgat agcggtactt atcaaatatt
    19021 gtttgctgca gcagatggtg tctttacctt cttacctgtc atgcttgctt ttacagcagc
    19081 taaaaagttc aaagccaacc agtttttagc agtagcaatt gctatggcac ttgtgtatcc
    19141 agctatcaca gcagttgctg gtgcgggaga agctatcagt ttctttggga tcccagtgat
    19201 tctttcacca tctggttata cttcttctgt cataccaatc attctagccg tatgggttca
    19261 aagtaaatta gagccattcg taaaaaaagt gattccgcaa tttctgcaaa tgattttagt
    19321 tccgcttgtc gttttagtag tgatggttcc attaacattt cttgctttag gaccaatcgg
    19381 gacggttgcc ggtaatgctt taggcggtct cttcaactcg atttatggat ttagtccaat
    19441 cgtagcaggt ctaatcatgg gaagtttatg gcaagtattc gtgatgttcg gcatgcattg
    19501 gggattcgta ccgatcatgt ttttgaatat cgaacaatat gggtttgatg tattgatgcc
    19561 aatgcttctt ccagccatct tggcacaagg tggggctgct ttagctgtag cattgcgaac
    19621 aaaagatacg aaacttcgag cattagggat ttcttctaca gtgacttcgt tatttggaat
    19681 cactgaacca actgtgtatg gtgtgacatt acctttgaag aaaccattca ttgccgcttg
    19741 tatttctgga ggtattggtg gagctattat cggattttcc ggagtaaaag ctttctccag
    19801 cagtttggtc agcttattga cgattccgac tttcattaat acggtggatg gtgtagagtc
    19861 aaatgtgaca gtagcagtaa tagctacagg aattgctttt gttcttgcat tcgtaggtac
    19921 attgatttta ggatttgacg aacaaacaca agacaatcaa ttagaaaaca aacatgcgaa
    19981 tgcaggagaa ccgattactt cagcacggca taccttaaaa agtcctttaa cgggtaaagt
    20041 actgccattg agtgaagtac cagaccaagt tttttcttct ggcgtgatgg gaaaaggtat
    20101 cgctattgat ccagaagtag gagaactagt tgcacctgca gacggagaga tcacgactat
    20161 ttttccaact ggacatgctg tagggatcac aacgactgac ggagcggaga ttttgattca
    20221 tatcgggatg gatacagtag aattaaatgg gaatggcttt gagatcttag tcaaacaagg
    20281 ggatctcgtc aaagcaggag atttattgat tcgttttgac atcgaagcaa ttagagcagc
    20341 tggctatagc gtaatcactc cagttgtcat tacgaatact gatgcatttg ccgatatact
    20401 tgaattagac caaaaagaga ttattgcaaa cgaagatgtt ctggcaattg taaaataagg
    20461 aggaaatcag gatgcaatca cgtttttcag aaaaattttt atggggtgga gcaactgctg
    20521 ccaaatcaat tagaaggggc ttatgatcga gacggaaaag gactatccgt tgcagatgct
    20581 atgcctggag gtaagcagcg ctttgcgatt atcggcagtg aagaatttga ctggacgatt
    20641 gatcaagaaa aatatattta cccgaatcat cgaggaatcg atcattacga tcgtttcaaa
    20701 gaagatcttg ccttgtttgc ggaaatggga ttcaaatgtt atcgtttttc gattgcctgg
    20761 acacgaattt ttccaaatgg agacgaaggt actcctaacg aagcgggatt agaattttat
    20821 gatcagctga ttgatgagtg cttaaaatat gatattgaac ctgtgatcac gatttctcat
    20881 tacgaaatgc ctcttcattt ggcaaaagaa tacggtggct ggaaaaatcg aaaactgatc
    20941 gacttctatg aacgatttgc tcagacagta ttagagagat acagcagtaa agtcaaatat
    21001 tggatgactt tcaatgaaat caattcggcc tttcactttc cagctttaag tcaaggatta
    21061 gtaaaaagca atggagcagg agagtaccaa aatattttcc aagcatggca taatcaattc
    21121 gttgcaagta gcaaagccgt aaaaatcggc cacgaattgc gttcggatat ccaaatcggc
    21181 tgcatgatta tctatgcaac aacttacagc atcgatgcca atccagtcaa tcaggcagca
    21241 acaatgatcc aaaaccaaga attcaatttt ttctgcacgg atgtccaagt acgtggagaa
    21301 taccctgcat acacagcgcg tacccataaa aaatatggag tcgatccgga aaaattagag
    21361 caaacggaaa aagacttcca gttgctaaaa gaatatccag tggactacat cggatttagt
    21421 tattatatgt ctacggcaat caatgagact gatccacaag cagcaacttc agaagggaac
    21481 ttgctgggag gagtaaaaaa tccgtttctt gaggcaagtg aatggggctg gcagatcgat
    21541 ccagaagggc tgagaatcgc attgaatgaa ttatacaatc gttaccagaa accattattc
    21601 attgttgaaa acggtcttgg agccattgat catatggaag aggatggcac gattattgat
    21661 gattatcgga tcgattattt acgtcgtcat atcgaagcga tggctaatgc agtagcagac
    21721 ggtgtagatt tgatgggcta tacgccttgg ggatgtatcg atcttgtcag tgcatcaaca
    21781 ggagaaatga gtaaacgtta cggctttatc tatgtggatc tagacgatga aggaaatggg
    21841 acactgaatc gatcaaagaa aaaatctttt gattggtata aagaagtaat tgcctcaaat
    21901 ggcgtaaact tgtaattaaa gcagctgaat gaaaaagaac acacttctca catgtgattt
    21961 gtgttctttt tttatttttt atgtgctatt cttttagtaa tagtaaagca aagggtgacg
    22021 ttatgaaaag gacacgtgta ctatacttgg aagtcgcaga taaaattaaa gaagatattt
    22081 tctcaggaaa atatccagtc ggttcgatgt tgccgacaga aagtgaatta gaagaactgt
    22141 ttcaagtcag caaaatcact gtccgaaagg caatcgaact gctggcatca gatgaatatg
    22201 tcgcaaagaa aagtggtcgt gggacgacgg ttctaagcaa tcgtccttac aatcgattat
    22261 ccaaagcggc aagctttacg caaatcttgg agaactcccg atatgaagtc cggaaagaaa
    22321 cgctggattt gcaattggtc catataaaga aggaagatcc tttgttttcc tttttcggtg
    22381 agcaagctgt ttgttttaag cgcttatatt atttgaacaa tcagccttat atttattttg
    22441 ttcattatct tccacaagaa atgatgcaat tcagcagaga agaatttggc aaagaatctt
    22501 tgtatcgttt gctgacgaaa aaaggatata ctattgactc attttctgat gatttctcgg
    22561 cagtattttt aacagaagaa gaacaaggaa ttctgaaaac agacgcacag atcggcatca
    22621 aacggatacg gaaatcgtct acgcaaattg gggaaatgat tgaatattcg gaggcggtct
    22681 accatacgga gctgcatcct taccatattg attatgaaac ataaataaaa aaagaggttg
    22741 tgaaaaagct gccctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt
    22801 tttcgtttgg ttaggcttat taccgcagat acacggctgt ttctggatta ccatttattc
    22861 tggataaggg ggtgtggcac gattttcaga aggaatctat gaaccaatca ctgtattgat
    22921 tttagcttcg atggtaaccc cattcggctt gacgatcgct tatttcttag ggaaaatcat
    22981 tcgtaaaaat atactcaatc gtcaagaaat cgatacattg aagaccgctt ttcctatggg
    23041 gatctgtcag atcacagagg gatgtttccc tatcgtattg aacgacttgg cacgcaatgt
    23101 catagcaacg ggagtcggtg ggacagtagg tggcggatta agcatgtttt ggggcgcaga
    23161 tagccgtatt ccggcatcgg gaatgtttgc agtcgctacg atgaccagac catgggcatt
    23221 cattggtgca ttgttagctg gttcttttgt cacaggaata accattttat tattgaaaaa
    23281 accagtagat ccgaatgcag aaatcatcca agaagaaaaa gaggaagaag acatatcttg
    23341 ggatgatttg acaattagtt aggagaggca gaatggatta caagaaaaaa gtacaataga
    23401 tgtaccttga gacaggaatg gtatgaaaga atcagagtta gaaagaatcg aatatacaga
    23461 ttttggttta ggagcgatcg aaactgtcca gagcatcggc accctccttg aaatgacgat
    23521 gaaggaaaaa tgagaacctt tcgttgtcga aagggtctgg tataccttat gtcgaaggaa
    23581 aggaaacaaa aaaactttcc tgtactattc caaaaaggaa agaaagttat tatacagtaa
    23641 aaaaagaaat tattcttcat gctagagatc agtacacgtt cgagcccaat atcaaacact
    23701 agttccaggc aggcgcaaca ggtgcggtta tcactgaatt ttcttccagc agtgacgatt
    23761 caagtgatat ttttacaaat ccagctattt atcgttaaca aaaagatttt taggagaagc
    23821 tgatgaacaa aaatgatcaa attttgatcg tttagttcgt cggctttttt atttagagaa
    23881 acatcattta tcagtgaaac ataacacgag aacaaaatga ttttttcgtt aataaaagaa
    23941 acaagtattt gtt
//
LOCUS       DAYYCW010000038        23322 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000038 DAYYCW010000000
VERSION     DAYYCW010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.038"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..1678
                     /locus_tag="ACHAYA_001354"
     CDS             17..1678
                     /locus_tag="ACHAYA_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8605158.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1748..2110)
                     /locus_tag="ACHAYA_001355"
     CDS             complement(1748..2110)
                     /locus_tag="ACHAYA_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM8605159.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            2178..3119
                     /gene="opp3b"
                     /locus_tag="ACHAYA_001356"
     CDS             2178..3119
                     /gene="opp3b"
                     /locus_tag="ACHAYA_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8605160.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3120..4163
                     /locus_tag="ACHAYA_001357"
     CDS             3120..4163
                     /locus_tag="ACHAYA_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8605161.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4169..5239
                     /locus_tag="ACHAYA_001358"
     CDS             4169..5239
                     /locus_tag="ACHAYA_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8605162.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5229..6182
                     /locus_tag="ACHAYA_001359"
     CDS             5229..6182
                     /locus_tag="ACHAYA_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8605163.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6194..7204
                     /locus_tag="ACHAYA_001360"
     CDS             6194..7204
                     /locus_tag="ACHAYA_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8605164.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7489..8310)
                     /locus_tag="ACHAYA_001361"
     CDS             complement(7489..8310)
                     /locus_tag="ACHAYA_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8605165.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8407..8685)
                     /locus_tag="ACHAYA_001362"
     CDS             complement(8407..8685)
                     /locus_tag="ACHAYA_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605166.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8953..10962
                     /gene="metG"
                     /locus_tag="ACHAYA_001363"
     CDS             8953..10962
                     /gene="metG"
                     /locus_tag="ACHAYA_001363"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM8605167.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11441..12028)
                     /locus_tag="ACHAYA_001364"
     CDS             complement(11441..12028)
                     /locus_tag="ACHAYA_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605168.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12203..13525
                     /locus_tag="ACHAYA_001365"
     CDS             12203..13525
                     /locus_tag="ACHAYA_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8605169.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13680..14456
                     /locus_tag="ACHAYA_001366"
     CDS             13680..14456
                     /locus_tag="ACHAYA_001366"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8605170.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14449..15018
                     /gene="rnmV"
                     /locus_tag="ACHAYA_001367"
     CDS             14449..15018
                     /gene="rnmV"
                     /locus_tag="ACHAYA_001367"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM8605171.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15011..15895
                     /gene="rsmA"
                     /locus_tag="ACHAYA_001368"
     CDS             15011..15895
                     /gene="rsmA"
                     /locus_tag="ACHAYA_001368"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM8605172.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16346..16975
                     /locus_tag="ACHAYA_001369"
     CDS             16346..16975
                     /locus_tag="ACHAYA_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605173.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17099..18208)
                     /locus_tag="ACHAYA_001370"
     CDS             complement(17099..18208)
                     /locus_tag="ACHAYA_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8605174.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18417..18839
                     /gene="mgsA"
                     /locus_tag="ACHAYA_001371"
     CDS             18417..18839
                     /gene="mgsA"
                     /locus_tag="ACHAYA_001371"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM8605175.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19026..19124
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19162..19698
                     /locus_tag="ACHAYA_001372"
     CDS             19162..19698
                     /locus_tag="ACHAYA_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8605176.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19738..21501)
                     /locus_tag="ACHAYA_001373"
     CDS             complement(19738..21501)
                     /locus_tag="ACHAYA_001373"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8605177.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21504..23219)
                     /locus_tag="ACHAYA_001374"
     CDS             complement(21504..23219)
                     /locus_tag="ACHAYA_001374"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8605178.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7498 a         4283 c         4758 g         6783 t
ORIGIN      
        1 taaaggggta cgtaaaatga aaaagaaatt tacatttggt ttggttgcgg tatgcggcct
       61 tgtacttgct ggatgttatg gcggcagttc agccacaagc agtagttcag caaaaagcgg
      121 ttcagcagat ggaggaggcg tatttaactt agtcgtgcct caagaaatgc ctacagctga
      181 cttatcagtt gcaacagata caatcagttt tacagcattg aataacgttt atgaaggaat
      241 ttaccgtctt gatgaagaca gcaaaccaca gccggcagga gctagcgagt tggcagaagt
      301 cagtgaagac ggattaactt acaaaatcaa attacgagaa gacgcaaaat ggtcaaatgg
      361 tgatcctgtt acagcagcgg attacgttta tggctggcaa cgaacagtag acccagctac
      421 agcttctgaa tatgcttacc tgtatgcgcc agttgaaaat gcagaagaca ttacagctgg
      481 aaagaaagat aaatcagaac tagggatcaa agcagttggt gactatgaat tagaaatcaa
      541 attgacgaaa caaacaccat atttccaata tctgcttgct ttcccatcat ttttcccaca
      601 aaaacaatcc gtagtggaag aaaatgggga tgcctatgca tctgcaagtg ataaagctgt
      661 ttataatgga ccattcacat tagaagattt tgatggacca ggaacagata cagaatggac
      721 ttataagaaa aatgatcatt actgggacaa agatacagta aaactttctg aaatcaaagt
      781 cagtgtagta aaagaatctt ctactgcatt gaacttattc aaagatggcc aagcagatga
      841 cgtgatcttg tctggtgagt tagcacagca aaacgcaaac gatccagctt atacttctgt
      901 taaagaagcg cggacaagct atatcgaatt caatcaacgt gaaaagaatt caccattcaa
      961 caatgtgaac ctaagaaaag ctatttctta ttctatcgat cgtgaagctt tagtaaaaca
     1021 agtactaggt gacggatcag ttgtctctac aggattgatc ccatcagata tggtaaaaag
     1081 tccaacaacg aacgaagact ttgcaaaaga agctggaaaa ttagtttctt atgacaaaga
     1141 taaagcaaaa gaatattggg aaaaagcgaa aaaagaattg ggcgttgatt ctttagaatt
     1201 tgatttgatg gcttctgatg atgattcatc gaaaaaagta atcgaatatg ttcaaaactc
     1261 aatccaagaa aacttggatg gcgtaacagt caaaccaaca cctgttccat tctctgtacg
     1321 tcttgaccgc tcaacttcgg gtgattttga cgcagtacta ggcggatggg ctgctgacta
     1381 tgcagaccca agcagcttta ctgatttgtt tgtcacaggt aattcataca accgtggtcg
     1441 ctggagcaat gcagaatatg acgaagcagt taaagcagct tcatcaacag acgctggcga
     1501 tgaacaagcc cgttgggatg atttccaaaa agcaatcaag atcatctctg atgatatggg
     1561 tgtcgttccg gtgtaccaaa aagcagaagg tcacctagtg aatgaaaaaa tcaaaggtat
     1621 cgttcaccat gcggcaggcg cttcatggga ttacaaatgg acatacgttg aagaataatc
     1681 gttaataccg tgagcatgag aaaaaacgag gctgcggcaa atgtcgcaac ctcgttctca
     1741 tattcactta tcctaacaaa gaaaaaagca tgaaaatagc agaaagagca attagtattc
     1801 cggcaatgat cagccaaaag ctgtacatac cgtagctggc agacgagagc gtggaaaatt
     1861 ctggtttttt ctttcggttc cttgttcgtg caagatggac cgatcgctga aaatgatcga
     1921 agaaacccga agaaaagacc cataaaaaac cgccgataat cagaaacggc aatgaccata
     1981 agaatagatc attagataat tgaatgagtg taaaggaatg gttgatgaga ttttttatta
     2041 aaataaccag aatcagtaga aaagctactg cgtaggggat agattttttt tcatttgatt
     2101 gctcctttat ttttggttca ttatctaata atgtagcgaa acatctgtca gaagtcaaaa
     2161 gctaggggga aacattagtg aatagttata tcaaatatgt attaaaacgt gtctttttca
     2221 tgatcatcac gctttggtta attgcaacaa tcaccttttt cttgatgcaa ttgctgccgg
     2281 gcacgccata tacgaatcag gaacgtttga gtcctgaaac aatcgagatg ctaaataaac
     2341 aagtcggttt ggataaacca gttatcgtac aatatgggat ttatctttct aatttgcttc
     2401 aaggagattt tggaatttcc ttccaattta aaaaccagcc agtcgcacat ttgttagctg
     2461 gaagaatcgg accatccttg cagttagggt tacaggcaat tatctttgga acagtcctag
     2521 ggacgatttt aggaacgatc tctgccatga agcaaaatac atgggcagat acatcttcta
     2581 cactcgtagc gatattaggt cgttccattc caaactttgt atttgctgtt ttattacaat
     2641 atatctttgc aatcaaatta caagttttgc caatcgcaaa atgggatggc tttgtgtaca
     2701 caatcttacc aacaattgcg ttggccatgt cgccgcttgc tgactctgct cggtttatcc
     2761 gtacggaaat ggtggaagta ctgcatagtg attatgtgga attagcaaaa gcaaaaggat
     2821 tgagccgttg ggaaatcgcc tttaaacatg gtcttcgaaa tagtttgatt ccattgatga
     2881 ctttattagg accgttagct gtagcattga tgaccggatc actggtggta gaaaatatct
     2941 ttgctattcc tggtatcggg gaacaattcg ttaagtcaat cacaacaaat gattatccta
     3001 cgatcatggc tgtaacgatc ctttactcat ttatgctgat ttttgttatt ttgatcgtgg
     3061 atctattgta cggtctagtt gatccaagaa ttcgtgtttc agaagggagc cgaggataaa
     3121 tggaaatgat accaaagcgt tatgaaacag ttgcagatat ccctgcggat gaatttcttc
     3181 cattacaaaa aaataccgaa gaagaacgtg aacagattac tgcaccttct ttgaatttta
     3241 tccaagattc atggcgccgt ttaaagaaaa acaaagctgc cgttatttca atggttttac
     3301 tattggtcat cattttgatc tctgtgatca caatctttgt ttctcctcat gatccaactg
     3361 cccaaaacgt ggcttatatc aatttgcctc ctcgtattcc gggaatcaat atcgatggat
     3421 tgaatggaaa agcaatggtt ggtggtgaac ttgttgataa atatgctcaa gcaaacgtcc
     3481 cttcaaacgt aaatttcttt ttaggaacag acggtttggg acgtgatgta ttaagccgct
     3541 tatttatggg aatgagaatc tcgttgctga tcgcatttat tgctgcatta ctagatgtaa
     3601 cgattggtgt agcttacggg ttggtttctg gtttgttagg cggacgtgtc gataacgtga
     3661 tgcaacgttt tttggaaatc ttatcaggaa ttccaaacct cgttgtcatg attttgatgt
     3721 tggtcgtttt tgaaccagga attttttcaa tcgtggctgc tatggccatc acaaactgga
     3781 taccaatggc acgtatcgtt cgtgcacaaa caatgaaatt aaaagatcaa gaatatgttt
     3841 tagctggatt gacattaggt gaatcaagaa tgaaaattgc attcaaacat attttgccga
     3901 atatctctag tgtcattatt attcaaatga tgtttagtat tccatctgct attttcttcg
     3961 aagccttttt aagctttatc ggtttaggtc ttacgcctcc gtcagcttca ttaggaacga
     4021 tgttgagcga agggtacaag acattcctgt atctgcctta tctattatgg attccggcag
     4081 taactttatc ggttatcatg attggcttta acttacttgc tgatggattg cgtgatgcct
     4141 ttgatcctaa aatgaaagag tgaagacgat gacgaaaatt ttagaagtga aagacttaga
     4201 aatctctttt gatacgtatg ctggaaaggt acgagcgatt cgcggtgtca attttcattt
     4261 gaataaagga gaaacattgg cgatcgttgg ggaatctgga agtggaaaat cagtgacgac
     4321 cagaagcatc atgcgcttgc tgtcaagcaa cgccaatata gatgctggtc aaatcctctt
     4381 taaaggtaac gatatcgttg gaaaatcaga aagagagatg caaaaaatcc gtggtaaaga
     4441 aatagcgatg attttccaag atccaatgac ctctttagat ccgacgatgc ccattgggaa
     4501 gcaagtcgct gagtccctta gaaagcataa taaagtttca aaaaaagagg gacaaaaagc
     4561 tgcattagaa ttgcttaaat tagttggaat acccgaagct gagaaacgta tcaacagtta
     4621 tccgcatcag ttctcaggag gtcagcgtca acggatcgtg atcgcgattg cactggtatg
     4681 ttatccggaa atcctgattg ctgatgaacc tacaacagcg cttgatgtga ctattcaagc
     4741 acagattttg gagctgctga aagaaattca gacaaaaatc gatacatcaa ttatctttat
     4801 cactcatgac cttggtgtag tagcgaatgt cgctgatcgt gtcgctgtca tgtatggagg
     4861 aagaatcgtt gaagttggaa catcggaaga aattttctac aacccgcaac atccctatac
     4921 ttggggactg cttggttcaa tgccgacatt agatagtgcg aacgatcgat tgtatgctat
     4981 tcctggttct ccaccggatt tattgaaccc gccaaaagga gacgcattct atccaagaaa
     5041 tgaattcgcg atgaaaatcg atgcagaaca agaaccacca ttttttgaac tatcaaaaac
     5101 acacaaggca gcaacttggc tacttgctcc gcaggcaccc aaagtgacac cgccagaaga
     5161 aatccaacgt cgctgggcta tctttaaaga gaaacaaaaa catacttacg gtcaaggagg
     5221 aattgctgat gccaaataaa ctacttgaag ttaaaggatt gaaacagtat ttcaatgtag
     5281 gaaaaaagaa tgaagtacat gctgtaaatg acatcagctt tcacatttat gaaggagaaa
     5341 cattcggttt agttggagaa tctggaagcg gaaaatccac tactggccgt acgatcattc
     5401 gcttgaatga acctactgat ggtgagatat tattcgacgg tcaagatata acgaagatca
     5461 aagataaaaa aggtttgacg aaattccggc atgatgttca gatgatcttt caagatccat
     5521 acgcttcgtt gaatcctcgt atgaaagtca gagatatcat tgccgaagga attgatgtta
     5581 atggactagc taaatcatct gaagagcgtg caaaaaaagt cgatgatcta ttgagaactg
     5641 ttggattgaa tccaagccac ggtactcgtt atcctcatga attttcaggt gggcaacgcc
     5701 agcgtattgg tattgcacga gcattggcag taaaaccgcg ttttatcatt tgtgatgaac
     5761 caatttctgc actagatgtg tcgatccaag cccaagtggt caatttgttg caagatctcc
     5821 aaaaagaaca tcaattgacc tatctattta ttgcacatga tttgtcgatg gttaaacata
     5881 ttagtgaccg aatcggtgta atgcataacg gcttgttatt agagatggga acaagtgatg
     5941 aaatctataa tcacggtgtt catccttata cagaaagttt gttatctgct attcctctac
     6001 cagatccaga ccatgagaga aaacgcagaa gaatcaaata tcaacctgaa ccagatgacg
     6061 gacaagtacg tcaacttcgt gaaatcgcac cagaacattt tgtctatgca actgagcagg
     6121 aagtcccata ttacgcaaaa aaactaaaac gacaaaagga atctcttctg gttgctaact
     6181 aggagggaca tagatgctaa aagcttttaa attccgtatg tatccgacag aagaacaaaa
     6241 acagcaattg attcgtactt ttggatgtgc tcgatttacc tataaccatt tgctgaaaaa
     6301 gcggcagaaa agttggcaac aaacaggagt agccaatttt tcgctcacac ccgctacatt
     6361 aaaaaaagaa tacccattct taaaagaagt agacagtctt gctttagcaa atgcacagct
     6421 taatttggat cgggcttttc gcaattattt caagggacgt gccagcttcc caaagcttaa
     6481 aacgaaaaaa agtatgtggc aatcttatac gaccaacaac caaacccgta cgatctatct
     6541 ggaaaatggc tacttaaagt taccaaaaca aaaagaacta atcaagataa atcagcatcg
     6601 gccagttgaa ggatcgattc gctccgctac gatttctgct cgatataatg aagaatttta
     6661 tgtcgcacta ctttgtgatg tttcgccagt aaaaaaagaa tctttagcta aatggatcgg
     6721 gatcgcctat catccgaaga cattgatcca aacatctcga cctctagaag taactttacc
     6781 aaaatttcac cagacagaag aaaagctgca acatgcacag cggaaattga atgtgaaagt
     6841 ccgctccgct caccatagaa agatcaggtt agatcaggca agcaattatc agaaacaaaa
     6901 gagaaaagta atggatcttt atctgaaaca aaagaaccag cgagaagatt atcttgaaca
     6961 attatctggg aaattggtca aacaatatga ctacttattt gtagaatctt ttccaaaaga
     7021 agaggcacat gctgattttt ctattcacga ctggcataaa ttaataacaa aattgcgtta
     7081 taagtcccaa tggtataata aaaaatttct tttgatcaat accgacggag cagaagaaag
     7141 caattccgta agaaagagcc aagtagtaga aaaaatgggc agacattcgg ttatcaaggg
     7201 ataaaaaatg aatgaaactg ataggagcgt tagggttagc cttgttaata aaaagtctta
     7261 atgacttttg tttctaggaa tttttctttt gaagaaaagc tcactaatta tgacaaacca
     7321 ataaaacaat agcagaaaaa gttcgtacga gctttttctg ctattgttaa ttttttattc
     7381 agaaagttta cgaatacatc aaaattctga aacaaaaaac gtaactcaca aagagttgtt
     7441 tttgcgtttt ttcttgaatg attaggtctg aatcttttgc tcacaacctt attcatggtg
     7501 ccccggcttg agctgataga tttttgaagg acggccgatt cctacaggac gttcaccgat
     7561 ctcatcgaaa tattggagca tagctttctt aaaattggaa tgatcgatcg ttttataatc
     7621 gatccctaag aatttagcaa atacttttcg agcttccgta atcgtgaaat ctttacctaa
     7681 aacttgtaga acttgcggct cgtgttccat tttattgact acacggttga atgctttcaa
     7741 gatgatttga ctatggtcaa aagccaaagt atctttgcca gaagactctc cagtgactaa
     7801 atcaagcgag atagccactt ctccgctaga taaatggatc ttgttactat gacgttctag
     7861 tgtgaaccag cgtacttcct tggcatcatc tccggcgatt agcggttctt ctccaataaa
     7921 ggctaagtag ctgactgtga cgacccagcc tcgcggatct cgattaggcg tactgaatgt
     7981 atgcagttgc tcaatatttt cttttgaaat aaccacacca gtttcttcct ttgtttctct
     8041 taagacgctt tctccagttg actcatcctt ttggacaaag ccaccaggca atgcccatga
     8101 atttcgataa ggatgccctt ttctttggat caacaaaact ttcagctgat cttcttcttt
     8161 gttatagcac atcagtacga tatcgacagt caaagagggc ttgtcatatt ctggacgttc
     8221 ctgtttttga taccagttaa gaaattcttc ttcacttgct ttatgttcat aatataattt
     8281 ttcttcttct ttagaagcaa aacggttcat ctgaccacct ccattgacag taaaagtact
     8341 ttttacctta ttgtagcaga ctctgaaaaa aaaccaagga tttacttgaa aaatctaggt
     8401 tatcatttaa atatgaaaaa taaatggaag tatgagattg aataaaaaga aaagcagata
     8461 aaacggcaat actttatacc gctcttcacg aatggcaaag aagccacaaa taacgatata
     8521 tacgtcaatg ataaaacgca aaatccaagc tgctgtcata aaactgttca aatgcccatg
     8581 gaggttaaaa taaccataca tacgaatata aaaatagctg aatattaaat gtgctaatga
     8641 aagaaaagca atactaagtg taaaaaaaag atgtttcttc tgcatgattg ttccagtccc
     8701 ttactaagat agatagtctt tattatacca cgtgtgaggg gagaaataaa aagccgtttt
     8761 ttagtagata tgaaacaaaa caaaaacccg catttattca acctattcat ccttttaaag
     8821 gatagattga acgtagatag aagtcgttta tccagtcttt ttcattttga aaaaaagcta
     8881 acagcatgct ttttttatgg tatactagca aggaaacgaa ttcgcatcat gcgataaagg
     8941 aggaagagcc ccgtggcaga aaaggaaact ttttatatca ctacgccaat ctattatcca
     9001 agcggcaaac ttcatatcgg caactcgtat actacgattg catgtgacgc aattgctcgt
     9061 tataaacggt tgatgggctt tgatgtgttt tatctcactg gagtggatga acatggtcaa
     9121 aaaatcgaaa caaaagcaga agaactaggt gtaaaaccgc aagaatatgt agataaaatg
     9181 gcggctgatg tcaagaaatt atggaaaacg cttgatatca gttatgataa attcattcgc
     9241 acgacagatg attaccataa agcagctgtt caaaaaatat ttgatcgttt attagaacag
     9301 ggagatattt acttaggtga atatgaaggc tggtactctg tttctgacga agaattcttt
     9361 acagaaacac aacttgcaga agtgtacaaa gatgacgaag gtaacgtgat cggtggtaaa
     9421 gcgccaagtg gacacgaagt agaattggta aaagaagaat catacttctt ccgcatgagc
     9481 aaatacgcag accgtctatt ggaatactat gaagaacatc ccgaattcat ccagccagaa
     9541 tctcgtaaaa acgaaatgat caacaacttt atcaaaccag gattagagga tcttgcagtt
     9601 tcacgaacaa cttttacttg gggagtacca gtcaagagtg atccgaaaca tgtggtgtat
     9661 gtatggatcg atgcattatc caattatatt actgcactag gatatggctc cgaagatgaa
     9721 agtctattcg aaaaatactg gcctgcagat gttcagatgg tcggtaaaga aatcgttcgc
     9781 tttcatacga tctattggcc aatcatgctg atggcccttg atttgcctct acctaaaaaa
     9841 gtatttgggc atggctggct attgatgaaa gacggaaaaa tgtctaaatc aaaaggaaat
     9901 gtcgtttatc ctgaaatgct tgtggaacgt tatggtttag atgcactccg ttattatttg
     9961 cttcgtgctg ttccgtttgg gtcagatggt gtctttacgc cagaagattt tgtttcccgt
    10021 gtcaattatg atctggcaaa tgatctaggg aacttattga atcgaacgat tgctatgatc
    10081 aacaaatatt gtgatggact cgttcctgac tatgcctcac ttgtcactcc atttgatagt
    10141 gaactgtcta cgacggctgc taacgttgtc ggacgttacc atgatgcaat ggaaaaaatg
    10201 gaattcaata cagcattggc tgaaatctgg gtgctgattt ctcgtgcaaa caaatacatt
    10261 gatgaaacag aaccatgggt attagcaaaa gacgaagaaa agaaagctga attgaatagt
    10321 gtcatgatcc atttagctga atctttgcgt atcgcagcga tattacttca gccaatcatg
    10381 acagaaacgc cagagaagat cttcaatcaa ctaggattgg actctgaaac aatgaatctt
    10441 gaaggattac actttggcga atttccttca ggaacaaaag tagttgcaaa aggtacacca
    10501 atcttcccac gtttagatat ggaagaagaa gttgctttta ttcaagaaaa aatgtctgaa
    10561 ggtactcaaa caaacgaaga tacagtcaaa tgggatccag aagaaacaga acttgtttca
    10621 acgaaagaaa aacaaatcaa atttgatgta tttgaaaaag tagaactaaa agtagcagaa
    10681 gtcatcaact gtcaaaaagt agaaggggca gataagttgc tgcaattccg actagatgct
    10741 ggagacagcc aagaccgtca aatcttgtca ggaatcgctg aattttatcc tgatccaagc
    10801 gaattgatcg ggaaaaaagt cgtcattgta gcaaatctga aaccacgaaa gatgcgtgga
    10861 caaatcagtc aaggaatgat cctttctgca gaagcaccag atggctcttt acaagtaatc
    10921 gaagcaccaa aatccatgcc aaatggctca gaaatcgcat agaaattcaa agggggtatg
    10981 acactagttg tgtcataccc cctttttccg aaatttgaga agcaattttc gtaaattcct
    11041 tcttattact tgaagccaat catataatac agaaacagag aactatccaa aataaatcct
    11101 agaaaaagaa gtacgagctt cgctgatttt tgttaattta gaataatact aataggaatc
    11161 gtaaaaacag aaaaaaagca taatgataaa gggtcgggca gattttaatt gattcatctg
    11221 gtactctttt ttattgtcct tacattcata aagtttgaat gcaatgactt tattacatct
    11281 atatttatat ctaacagtag cgttgatgtg ttaattataa gttctatcac tttatgttca
    11341 attagtttgt gctcttatat gtgtgattga aaatatagat aaaaaaacaa tcatcggctt
    11401 tgacttcgat gattgttttc tttattttgt aaaaatccta tcacttgatt ttatggtaac
    11461 cttttatttt tgtcttcaaa cggcttggta agagagcagt aagcagtgca aatgggatcg
    11521 aaaagacata ccagaaaatc gtacgtccga taccataatg ttgatagagc tttgtcaaca
    11581 tcacgtgtaa caagatcaac cctaaaagca aggagccagc tataaaccaa tgattatccc
    11641 aatgcatgac ttcactaagt gccatcactc caaaaggata gaaataaaaa tagatcccga
    11701 ctaaggtcat ccgccagttc tttgtaaatg ttccgatatt caataaaaag aaaatcgctg
    11761 aaagcaaaat accgataggc ggaaaaagcc caaccaaaaa ggaatacaac atcccccaca
    11821 agatcatcac aggacctttg atcaatgcaa cgattgcgat gaacaaagtc agcaatatta
    11881 gtttttgtga taacgttgca acagtcatta atccaaataa aattaatgtg aaccaatgcc
    11941 aatgggactg ctgttgttct ttacgggaaa aatcgacccg gttggataaa taatctgcta
    12001 atttcggata agaacgctta aaatccataa actgttcctc cttcgtggaa aaggtaggtt
    12061 tagtatacga aaaataaaaa agaaatacat ccgtcttaag agggagtttt tcactgaaag
    12121 tggagaaaaa agaatggtaa attaaaatat tctcagttat actgaaaatt ataccaaatt
    12181 tcaaagaaga aagaggatgg gaatgaaagg caaactaaaa cgagaaatca atctgtttgg
    12241 cgcattagct accgttatgg gaaccgtgat cggtgcaggc gtcttcttca aaactgctgc
    12301 tgttactgct agtactcagt ctacaagttt gacattgcta gcttggcttt tgggtggttt
    12361 cttaacgatc tgtgctggat tgacagtggc tgaattagct acagcaatcc cggaaactgg
    12421 tggagctgta aaatatattg aagctgcgta tggaaaattg cccagctttt tattaggatg
    12481 ggcgcaaagc ttgatttact ttcctgctaa tattgctgca ttatcgatta tctttgcgac
    12541 acaattgacg aacttattac aattatctac cgattatctt ttgttgattg cagtgatcac
    12601 cgctgtttct gtgacagggt tgaatttgtt agggacaaaa gtcggtgctt ctgtacaatc
    12661 agtgacgttg attgttaaat taataccaat tgctgtgatt gtcatatggg gattattgac
    12721 cccaggacaa ggaactgtcc agttatttcc aatcgaagct ggaaaagatg ttacttttgt
    12781 cgaaggattg agcagtgcgt tgttagcaac gttgtttgct tatgacggct ggttaggtgt
    12841 cggtgcaatg gcaggggaaa tgaaacggcc ggaaaaagat ttgcctaaag caattattct
    12901 tggtttgagt tttgtaacag tcgtttatct attgatcaat tttgtttttt tgaaaacttt
    12961 gccgattgat catcttgccg gtaatttgaa tgcagcttct gaagcatcag acgtcatttt
    13021 tggaggaatc ggtggaaagc tggtcactat tgggatcttg atctcggtat acggtgcatt
    13081 gaacggttat acactaactg ggatccgcgt gccttatgcg atggctttag aagacgagct
    13141 acctttcagt aaacagctga caaagctttc aaagaagttt acggttcctt atgttcctgc
    13201 ggttttccag ttagcagtag cttgtattat gatgagcctt ggttctttcg actttttaac
    13261 agacatgttg atttttgtga tgtggctatt cagtttactg atttttatcg gtgtctttat
    13321 tttaagaaaa aaagcgccgg aattgccacg tccatataaa gtaccacttt atccaatcgt
    13381 tccgattatt gcgattctcg gtgctatttt cattttaggt atgacgatga tgacgcaaac
    13441 gaaattagca ttgatcggta taggtgttac cttgataggt atcccagtct attatcaaaa
    13501 aaagaaaaaa cgctcagaag agtaaaatat ttcaacaaca gacagaaaaa gtacgaacta
    13561 ggtcctctag atcttcgtgc tttttctatt tcaataaaac aagttcttct attttgattt
    13621 ttgctacttc tgcaagtagt gcttatgtgc taaagtaaga atgaaaagga gagataaaca
    13681 tgatatttga ttctcatacc catttaaatg ctgaacagtt caatgaagat attccagaaa
    13741 cgattcagcg agcacaagaa atgggggtaa cgaaaatggc ggtcgtgggc ttcgatacgc
    13801 caacgattga aaagtctttg caattaagcc atgactatcc aaatatatac agtattatcg
    13861 gctggcatcc gacagaggct ggcagttata cgaaagaaat cgaaaataaa ttacaagaac
    13921 aattgacttt accaaaggtt gtagcattag gggaaattgg cttggattat cattggatgg
    13981 aagacccgaa agaagtccaa gataaagtat ttcgcagaca gattgctatc gcaaaagaaa
    14041 tgaacctacc aatcagtatt catactagag aagcacttga agacacgtat caaatcctaa
    14101 aagaagaaga tgttcgagat atcggtggaa tcatgcatag tttcagtggt gattatgaat
    14161 gggcaaaacg atttcttgac ttagggatgc atatttcatt tagcggtgtc gtcactttca
    14221 aaaaagcatt agacgtccaa gaggctgcag caaatgtacc aatcgaacga ttacttgtgg
    14281 aaacagatgc cccttatctg gcaccagttc cttttagagg aaagcgaaat gaaccaggct
    14341 atactcgtta tacagtagaa aagattgctg aacttcggaa tcttccgaca gaagaagtgg
    14401 ctgcccaaac atggacaaat gcgcaccgat tatttggtct aagcgaacat ggataagatg
    14461 aagatccaag agattatagt cgttgaagga aaagacgata caagaaggct gcgggaagta
    14521 gttgatgcag atacgataga aacaataggg tctgcaatca atgaagagat cctgatgcaa
    14581 attgaacatg cccaagagac tcgtggagtg atcgttttta ccgatcctga tttttcagga
    14641 gaaaaaataa gaaaaatcat tatggaagct gttcctcagg caaaacatgc ttttttgccg
    14701 cgcacacaag ctaaaccaaa gaaaaaggga agccttggtg tcgagcatgc aagtgatgct
    14761 gctattttag aagcattgaa aaaggtcgtg acgccagaca tttcccaaac ggctgtttct
    14821 gaaatcacta gacaagacct tttagactat ggtttaatag ctggggcaca agcaaaagaa
    14881 aaacgagaaa aactaggtga cgagttgcgc atcggttaca cgaacggcaa acaattggaa
    14941 aaacgtttga agatgtttcg tattacgccg aaagaactcg cccaagcaat gaaaatagtg
    15001 gaggaacaac atgagtgaat atcgtgaaat tgcgacaccc tctagaacaa aagagatttt
    15061 attgaaacat ggtttttcat ttaaaaaaag tttaggacaa aattttctga ctgaaccaaa
    15121 tattttaagg aaaatcgtag aaactgcgca aatagatgaa catacgaacg tgatcgaagt
    15181 tggaccagga atcggtgcat tgacggaaca attagccaag catgcaaaac aagtcgtagc
    15241 atttgagatc gatgatcgtt tgatccctgt attggctgat acgatgcaac cctatgacaa
    15301 tgtgactatt gtacatcaag atattttaaa gaccgatttg tcaacagctg ttagagaaac
    15361 atttcatgag gagcttccgt taaaagtcgt agcgaattta ccttactata ttacgacacc
    15421 gatcatgatg cattttcttg aatcagattt ggttgtggat gaactagtcg tcatgatgca
    15481 aaaagaagta gctgatcgta tctctgctga accaggaacg aaagcttatg gttcattatc
    15541 gattgctgtt caatattata tggaagccag tttagcattc atcgtaccaa aaacagtatt
    15601 tgtccctcag ccaaatgtag attcagcgat tttgaaactg acaagaagag atataccggc
    15661 agtcgaagtg acagacgaaa aagcattttt ccgattgaca aaagcggcat ttcagcaaag
    15721 aagaaaaacg ctttggaata acttgcagca ttcgtatggc aaagatgacc agacaaaagc
    15781 atggttagca aaaagcttgg aaacagctgg aatcgatcca aaacgtcgtg gggagacttt
    15841 gtcattacaa gaattcgccg cattaagcaa tgcgatgtca gaaaatatgc agtaaaaaaa
    15901 gaggttgtga cagaagttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac
    15961 aaaaatagtt cctcatgttt ttcgtttgtt tgggcttatt accgcagatg caagcttttg
    16021 aacaccgttt aagaaagagg ttgtgggcct gccgtttaac tctaagtcac aaggaacaat
    16081 ttcaaaaaac agcttctcat gtttttgagt tagttggtct tattacttga tgcaagcttt
    16141 tgaccactat tatgccgaac aaggagatgt gacacaaatt ttgtcacatc tcctattttt
    16201 gtttcttgat caaaaaattc ttatacttga tactcacact cattttgtat ggatttagaa
    16261 aagaatatgt gtcttgacag ttattagtaa gtgagttata ctaagtcgac atttatgtgt
    16321 caagacatat taggagagag aaaaaatgag aaaaaattca gttcgaaaac taacgatttc
    16381 tgctttattg attgggatgg gggtcattat cccaatggtc atgcctaaaa tcatgattcc
    16441 accagcttcc tttactttgg cgtctcatgt gccattattt attgcgatgt tttttacacc
    16501 aggagttgca gtggctgttg cgctaggtac gacatttggt ttctttttaa caacgccggt
    16561 tatcatcgca ttgcgagcat tgtcacatct tgtgtttgcg ttgattgggg cttggtactt
    16621 gcaaaagcat ccgagtatcg ttttaaaaaa tggacagttt acttttgcta attggcgttt
    16681 ccaagggttc aattttgtga tcggtgtgat tcattcttta gcagaaatgc tagtagtcag
    16741 catcttttac tttattggta atatgcctga aacatattat tctcaaggat atttttatac
    16801 agtattcatc ttgatgggga ttggtgggct tatccacagt ttagttgatt ataatattgc
    16861 ttattttata gcaggaacac tcagcaaata ttttgatatc cccgtattta cggcagcaaa
    16921 aaaagaaaat cggaaagagt cgattcaagg gtacgcgaag aaaacgacat attagacatg
    16981 atctctcaaa aaaaaaactc tcatctagta atggataata aatcctctaa gatgagagtt
    17041 ttttgtccta aaaagagctg caccaacatc ttctggcaca gctctaacag ggggcaattt
    17101 atggaagttt gatgacttgt tctgtttctt tatcaaagaa gtggcctttg ctgatgttga
    17161 aggcaaggtc gactatttcg cctggtttat ggaaatctct tgcatctact tttgagatga
    17221 actcagtaga accagttttt gtgtaaagca tcgtttcagc tccaagtaat tcggaaacga
    17281 caacttctga tttcactacg gcttcaggtg aagcttcaag ggctacttgt tcactgtgga
    17341 tgtcttctgg acgaataccg aagataactt cttttccttc ataacctttt tcaactaata
    17401 ctttgttttt tccttcagga atacgaagct taagcccatg tccatctgtg ataacaccat
    17461 ttttcaatgt cacattaaag aagttcatgg caggagatcc aataaagccg gcaacgaaca
    17521 tattgacggg tgtatcataa acttctttcg gtgtaccgat ttgttggata aatccgtctt
    17581 tcatgataac gattcggtca gccatagtca ttgcttctgt ctgatcgtga gtaacataga
    17641 tggtcgttgt ttcaagacgc tggtgaagtt tggcaatctc agcacgcatg gctacacgta
    17701 atttagcatc taaatttgat aatggctcat ccattaagaa gacttttgca tcccgaacga
    17761 ttgcccgtcc caatgccaca cgttggcgtt gtccaccaga taaagcagct ggttttcgtt
    17821 tcaagtattc tgtcagacct aagatctctg ctgcattgtc cacacgtttt ttgatgtctg
    17881 ctttatcata tttacgtaat ttcaagccaa aagccatatt atcaaaaaca gtcatatgag
    17941 gatacaatgc gtagttttgg aaaaccatgg caatatcacg gtctttaggt gccacgtcat
    18001 tcatgacttt tccgccgata tttagttctc cttcagagat atcttccagt cccgcgatca
    18061 ttcgcaaagt agttgacttt ccacaaccgg aaggaccaac aaaaacgata aattcacggt
    18121 ctttgattga tagattaaaa tcagttacag aataattttc tgcattgtca tattttttat
    18181 atacgttttt cagagccatt tctaccataa tacgttccac actttctttt ttagttttat
    18241 taactattta aagtataaat gaaagcgtta actaacttca atgtcatgtt gaacaagaat
    18301 tttttctttt ttttgtcaat gtgctagttt taagctttaa taaatatcca taagaaataa
    18361 aaactgatac actggagtaa ataagtatgt taacaatatc aaaaggagtg tctcttatga
    18421 aaatcgcttt gatcgctcat gaccgaaaaa aacctatgat ggtgaaatta gcaacagcgt
    18481 ataaaacaat attggaaaaa catgaacttt ttgctaccgg gacgactggg cagaggatca
    18541 cggaagcaac cggcttgccg atccatcggt ttaaatccgg accgttaggc ggggatcagc
    18601 aaattggagc catgatttcg gaggatcagt tagatttggt gatttttctt cgagacccgc
    18661 tggctgcaca gccccatgaa ccagatgtaa cagcacttat ccgtttatct gatgtctatg
    18721 agattccgtt agctacgaat atcggaacag ctgaggtgct actaagagga ctggaagccg
    18781 gcttcctaga ttttcgaaga atcgtccatg aaatagacaa gcatccactt tcattttgat
    18841 ggataagcgt atggaaaaat aacgtttaat gttcgcctat atgtagctat aaaagctaaa
    18901 caaactgata atatgcgata taatacgaac gtattggata atataaacaa taatattagt
    18961 ttttcgtatt tgcagtaaat gtgaaaatat gctatagttt ctgtgggaaa agatcccaaa
    19021 aataatacag agtagaaaat aaagcgttaa gtgctaagag gatgggatgt tgcctcttgg
    19081 acgaaggacg taatgttcgc ggttttattt tcgcattcgc tgcatgatac gatgtagcag
    19141 ctcttaaagg agatgctaaa aatgaagaaa caccggttag acgcacgtat gattgctatt
    19201 atgggcttat tgattgcatt gatggtcact ttgtcccgac tggtagcaat cgagacccca
    19261 tttattaaaa tcagtgtcac atttattcca caagtgatca tgggcattct ttttggtcct
    19321 ttttggtcag ggataggcgc agttttagct gatttagtag gaatggccct cttctcgaaa
    19381 tctgcattct ttattggatt tacattgaat gcatttattg aaggagcaat ctacggattc
    19441 tttttctatc gaaaagagat cacttggaaa aatgccattc tggctacgct tagtgtcacg
    19501 ctgatcatca acttattttt gacaccatta tggcttgcgt tgatgtacca tgtgccattg
    19561 ttcagctggg tcgtttgggc gccccgcctg ttaaaaacag taatatggtt accgattcaa
    19621 tcaattgcca tttattacgt aggacgtagt attccttaca aaaaaatttt aagaagttta
    19681 gcgatccacg ctaaataaaa aacaagcagt cttcccatcc cagaagactg cttgttttta
    19741 ttgtgctgtg aattgtgctt gatagagccg tcggtaataa ccatcttttt gaagcagctc
    19801 atcatgggtg cccatttcga caatagctcc ctgatccatc actaatatct ggtccgcatt
    19861 tttgattgtg gacaggcgat gggcaatcac aaaactggtt tttccttcca tcattgctaa
    19921 gaaggcttgt tggatttttt tctctgtcaa tgtatcgact gagctggtag cttcatctaa
    19981 gatcaacata gggggattac tgatcatcgt tcgagcaatc gtcaacagtt ggcgttgtcc
    20041 atctgatatc tttaaccctt gaccgccaat cttagtatct aatttttccg gcaaacgttc
    20101 tacaaattca tacatataag aggcttttag ggcatcattg atttctgcgt cacttgcttc
    20161 cggattccca aaacgaagat tttctcgcaa tgtactgtca aatagccaag tatcttgtaa
    20221 caccattcca aaacttttac gtaatgtatc acgttggata gagcggatgt catggtcatc
    20281 gactgtaatt gttccttttg ttgtgtcata aaaacgcatc agcaaattaa caagtgtgga
    20341 ttttccggcc cctgtttttc ctactatagc aatggtttga cctggataag cagtaaaatt
    20401 gaaatcttcg atcaaacgct gcttggggtc ataagaaaaa gaaacatgat caaaagagac
    20461 ttttcctttg atatttttgg gttccaaggc ataggcatct ggcaaatcag gcttttcttc
    20521 tggctgatcg atcaaatcaa atgctcgttc tagaccagaa aaagcggttt gcagttgtgt
    20581 cgtgattcca gaaagttcga taaatggttt agaaaactga cttgagtaga tcgtaaagct
    20641 ggaaatcaca ccaacagtga ttgacgaacc actcttcaat gccaataggc cgccaactaa
    20701 accaatcgat aaataagcaa ggtgatctac aaaacgagaa gacgggtttg tcaaggaaga
    20761 agaaaattgt gcccgttgtc ctttttcata tagatcagca tttagcagtt caaattgctg
    20821 ttgactagct tcttcttgct gaaaagcttt cacgattttc tggttaccga ttcgttcggt
    20881 cacaaagccc gagatatccc caataatcct ttgctgtcta tcgaaatttg tttgtgaagc
    20941 acgagcaacg atccaactca caaggaaaat aattggtgta gaacaaagaa cgaccaatgt
    21001 cagaagtgga cttagacgca tcataaaaat aaacgaaaga atgatgattg ccactccaga
    21061 aaataattga ttgaaggccg cagaaattgc aatggaaata ttgtccatat catttgtaaa
    21121 tcgactgacg atatttccgt gggagttttg atcataataa cgaagtggca atcgattcag
    21181 atgagcaaat gcatcttttc ttaattttgc aacggataag taagctaacc gattgcttaa
    21241 cacttggatg agccattgac tgactacgtt gattaaaacg ataccaccga atagaataag
    21301 taaatggaac agtcgattga agcggacctg tccttctcca atcatttgat cgatactttg
    21361 accaatttgt aacgtcatca caactgttgc aatcccattg atgattccta gtatcacagc
    21421 tagccaaatg tcttttgcat aggcagtaag gtaaggaatg aatcggcgga gcgtctgcaa
    21481 ggataatttt tttactttca taactattct tcctcctgtg atgcgacgat ttcctgatat
    21541 tctgctgatt gagcaagtaa ttcttcatgg gtaccctgag cagcaatctt accttgatcc
    21601 atcactaaga tatgatgtgc ttcttgaata gagcggacac gttgggagat aataatcact
    21661 gtactgccaa gataagtctg tagcgcctta cgcaaatcca agtctgtttg ataatccaac
    21721 gcacttaaag agtcatctaa aatcagtaat gctggcttgg caatcaaagc tcttgcaatc
    21781 gtcagtcgct gtttttgtcc gccagagaaa tttttcccat tttccatgac ttttgtatct
    21841 aaaccatttg gaagctgacg gacaaattct tctgcctgag cgattttcag agcttcccaa
    21901 cattcttttt ctgtggcgtc tttcttaccc cattgaaggt tttcacgaat agtcccggta
    21961 aatagtacag aagtttgggg aacaagagca atttgctgac ggagatgatc cagctgccaa
    22021 gaacgaacat ccattccgtc cattataact gtaccggaac tgacatcata gaaacgcgga
    22081 atcaattgtg tcaaagtcgt tttcccgctt ccggttggac caatgattcc aaggactgta
    22141 tttcgcttca gctgaaatgt aatattttgg agagaaagac cagaatcagg agtataacgg
    22201 aagtcaacat ggtcaaaagc gataattgct tcagaagaaa aatcgggagt agtactgacc
    22261 tcagcttcaa gaatcgaatt ttctgtgtct agcacctctt tcacccgatt tcctgaagct
    22321 tcggcacgtg tgaagatcac gacgaggttt gaaacaacga tcagtgccaa aagcatttga
    22381 ttcatataat tgatcaaagc aagaacttgt ccttgttgca agctaccaat atttactttg
    22441 atcccgccaa ctgtcagcaa acagatgatc ccgacattca tgatcaaggt tgtagcaggt
    22501 gataataggg cagagatatt ggaaacacgt aaataattgt tcgctagatc atttgtgctt
    22561 ttatcgaact tctctgtttc tttttttgtc tgcgcaaatg cgcggatgac acgaacaccg
    22621 ctaagattct gactaacgtg ttcattgagc tgatctagtt tttcttgcac tttttggtag
    22681 agaggaacgg tttttttgat gataaaaaat aaaatgattg agaaaatagg caataatgct
    22741 aagaaaaaca gacctacttc ccagtcgatc acaaaagcca tgaccactga cccgatactt
    22801 aaaaacggtg ctctgatcaa taaacgaatg accattgcta aagctagctg aagctgattg
    22861 atgtcgtttg tcattcgggt gatcaaggta tctgttccaa aatgattcaa ctcgttgtga
    22921 gagaaagtat tgattttttt gattagctga ttacgtaact ctgtaccaaa tccttgtgaa
    22981 gctactgaag catagtattg gcaaatgata gcacaaatca gaccaatcac agacatagcg
    23041 accatccata agcccatttc aatgattttt cctcggtcat tcatttttag tccattatcg
    23101 atcaaggaag ccatcattaa aggcaggaca agttcgaaga cagcttcaag aaatttgaag
    23161 aacgggccca aaatgatttg cttacgatag tcttttgcat aacgtaataa ctgaatcata
    23221 tgattcctcc tattatttat ttgtccacaa ttgaaaaaac aagtactacc acattgtagc
    23281 ttaggaaatt tagaatgaaa agagaacaag ccttttcata aa
//
LOCUS       DAYYCW010000039        23126 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000039 DAYYCW010000000
VERSION     DAYYCW010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.039"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..205)
                     /locus_tag="ACHAYA_001375"
     CDS             complement(<1..205)
                     /locus_tag="ACHAYA_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605179.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVN"
     gene            complement(212..760)
                     /locus_tag="ACHAYA_001376"
     CDS             complement(212..760)
                     /locus_tag="ACHAYA_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM8605180.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(805..1137)
                     /locus_tag="ACHAYA_001377"
     CDS             complement(805..1137)
                     /locus_tag="ACHAYA_001377"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605181.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1151..1408)
                     /locus_tag="ACHAYA_001378"
     CDS             complement(1151..1408)
                     /locus_tag="ACHAYA_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605182.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1603..2145)
                     /locus_tag="ACHAYA_001379"
     CDS             complement(1603..2145)
                     /locus_tag="ACHAYA_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFM8605183.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            complement(2166..3257)
                     /locus_tag="ACHAYA_001380"
     CDS             complement(2166..3257)
                     /locus_tag="ACHAYA_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8605184.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3265..5475)
                     /locus_tag="ACHAYA_001381"
     CDS             complement(3265..5475)
                     /locus_tag="ACHAYA_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8605185.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5491..8019)
                     /locus_tag="ACHAYA_001382"
     CDS             complement(5491..8019)
                     /locus_tag="ACHAYA_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8605186.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8071..8481)
                     /locus_tag="ACHAYA_001383"
     CDS             complement(8071..8481)
                     /locus_tag="ACHAYA_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM8605187.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(8494..8712)
                     /locus_tag="ACHAYA_001384"
     CDS             complement(8494..8712)
                     /locus_tag="ACHAYA_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFM8605188.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(8709..9701)
                     /locus_tag="ACHAYA_001385"
     CDS             complement(8709..9701)
                     /locus_tag="ACHAYA_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8605189.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(9780..10691)
                     /locus_tag="ACHAYA_001386"
     CDS             complement(9780..10691)
                     /locus_tag="ACHAYA_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8605190.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVSAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(10710..11054)
                     /locus_tag="ACHAYA_001387"
     CDS             complement(10710..11054)
                     /locus_tag="ACHAYA_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605191.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(11074..11352)
                     /locus_tag="ACHAYA_001388"
     CDS             complement(11074..11352)
                     /locus_tag="ACHAYA_001388"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605192.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(11711..12211)
                     /locus_tag="ACHAYA_001389"
     CDS             complement(11711..12211)
                     /locus_tag="ACHAYA_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605193.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(12212..12757)
                     /locus_tag="ACHAYA_001390"
     CDS             complement(12212..12757)
                     /locus_tag="ACHAYA_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605194.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(12754..14010)
                     /locus_tag="ACHAYA_001391"
     CDS             complement(12754..14010)
                     /locus_tag="ACHAYA_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8605195.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(14370..14681)
                     /locus_tag="ACHAYA_001392"
     CDS             complement(14370..14681)
                     /locus_tag="ACHAYA_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605196.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(14704..16179)
                     /locus_tag="ACHAYA_001393"
     CDS             complement(14704..16179)
                     /locus_tag="ACHAYA_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFM8605197.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(16628..17080)
                     /locus_tag="ACHAYA_001394"
     CDS             complement(16628..17080)
                     /locus_tag="ACHAYA_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605198.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(17082..17447)
                     /locus_tag="ACHAYA_001395"
     CDS             complement(17082..17447)
                     /locus_tag="ACHAYA_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605199.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(17546..21829)
                     /locus_tag="ACHAYA_001396"
     CDS             complement(17546..21829)
                     /locus_tag="ACHAYA_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFM8605200.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21976..22215)
                     /locus_tag="ACHAYA_001397"
     CDS             complement(21976..22215)
                     /locus_tag="ACHAYA_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605201.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(22305..22547)
                     /locus_tag="ACHAYA_001398"
                     /pseudo
     CDS             complement(22305..22547)
                     /locus_tag="ACHAYA_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22660..22896)
                     /locus_tag="ACHAYA_001399"
     CDS             complement(22660..22896)
                     /locus_tag="ACHAYA_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605202.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         6804 a         5009 c         3396 g         7917 t
ORIGIN      
        1 cattgactat aactagttca tctggtaaaa aaattaacca acaaacatcg acaaacagta
       61 aatgatataa tccatttcca cctccaagca tattcaatac tggtgaacat gccttagaaa
      121 cacgaatata gttatggttt aaatagatat tttctttaaa cattgcttgt ttaaaagcca
      181 tatgctcttg ttctaaactt ttcataaact tttactctaa tgcacagcta gcagcagctg
      241 ctgcaattcc tgctacaata tttaccttag ctaactcttt agctgcagct tttcctaatt
      301 gtttttctgc aatttttaat aataaatccg cagctttttc aaaattaaaa gcttttaatg
      361 cttcaataaa atctccctgc gtacctatag cccaagccac tcctcctcca ggaagagaat
      421 taatggctac acatccagca tatcgtttcc aatcaacttt ctccattcta ggaacaataa
      481 tatgttcata aaagactctt gctgctcttc cttgttcgtt atcttgactg attcgttcac
      541 gaatagcacc aaaatccttt atatggtata caccatgttc gtctaaaaaa ccaactgtat
      601 caaaataaaa agttaattca tctactaatt tattctttat ttctaaatca actgtattca
      661 ctttttctgt ggcatacaca cttgttccag tactaacaaa agtacttaaa cctaataaga
      721 aaacacctaa aacaccaaat attttaatta ttcttttcat aatttctcct ccataaaaaa
      781 taagttactt tttaaatatc ttgtttatga attcattccc caaaggcctt ttaagattga
      841 tacagcagtc cgttcacttc ttttagactt gtcatacaag tcttttaaat gatcccatat
      901 cagactagtt tgtctatcta gcttcaatcc attaaacgct aatgaacggt tcattttttc
      961 tattacttta gataaagcta cagaaatttt tataccttca caacgcgcat cttggatttc
     1021 aataatagta tcatcaatta gtttttgaat ttcttgccta tcaacatatt caatcttaga
     1081 aagagaatct tttaaagcta ctaatgattc aacgatttct tttttctctt cagacatttt
     1141 cttcctcctt ctagtgaccc gtaaagccat gtagagcaga gtccattgaa ccagtaatta
     1201 cactatctac aacgccattc caatttactc tacattttcc atttttcaga tttgtcggtc
     1261 catacggata gcctttttga cattcaatgt tcggattata ccagatagga ttataccatt
     1321 cagccgcaga agcagattca gtcgatacaa acaatcccac accaacaaat ccaatacaca
     1381 tcacactata gccaattagt tttttcatct cgtatccatc tcctaaaaaa tagaatagtt
     1441 cttatagtta ttcataagac atattttttc cgtttttgtt ttatcctttc tgtcctataa
     1501 caactttttt caagtaaaga atgacatagt ttttcttaaa aaaacagtct gtttttttct
     1561 atttcttcgg tgtataataa atatgagtgt tcatttttta ttttaatacg cactccgtac
     1621 ataataaatc ttgatcgcat caatcttttg agcttcttga cctactttca attgaagtaa
     1681 tacaaattga gtaaccttat tcattgtttc ttctccaaat aacaaatatt gatccggatt
     1741 ctctatatct tgtgtcacct ggtaaagttt cccagttgtt ttcatttgta catgcgggtc
     1801 tacatcaata ctattgtctt taatacaacg atctgtcata tagtccctca cagcttgatt
     1861 gcgtttataa acatctgtga aattactcca ttttctagca aaatctttta agaaattttt
     1921 tgtcatttca tctatttgca tttgttgcgc tgtctcgtct tctaatgaac caatttgact
     1981 gttactttca gtactttgaa gagttccact tgtttctttt actgcttcga ttgtttcttc
     2041 tgtcgttctc aattgtgttt tttgtttttc agaagaaaac caatgacaac cagtaagacc
     2101 tatcaaacat aaccaactaa atactaaaat tatccctttt ttcacgtttc tagtcactgc
     2161 ctttcttatt tcaccttacg aaccagacta cgaatttttg tttgattgta cgtgcgttga
     2221 taccgagcca taatccgtcc ttgctcggca ttctgttcca cactagagaa atgttgtccg
     2281 ccatatgaaa catctgttat aattccggtg tgaccataga ttccaggaga caatgcacca
     2341 cctgcttgcc aattcaccac atctcctgct tgtaaatcac ttggttttgg gttcatgatt
     2401 acttgccaac cataggttgt ccagttataa tcttccccta ttaacatcgc aaacatgtgt
     2461 ccagaaccca ttaactgtgg gccacctaat tgagatacat accatgccgt accaccataa
     2521 cattccccat tatttacgga tcgtccaatg atccgatcca aaaccttcgc attgccttta
     2581 ctttttcctg caataagcga agtttttttt gttggtaggc tattccaatg cttgatcgca
     2641 aaagtatcat attccgttaa gttataggac gttatgatac tcattgctcc tcgataatac
     2701 ggactccctt cggtaatacc tgctgccgca tagcctccac gacctagaat caataaacaa
     2761 cttccaggat ctttattgcc cacacattga ttaaagttgc tttggctcgc caccataaag
     2821 ccataatagt cataacaagc ttttaagttc ggaaacttca tatagaaacc accttcagaa
     2881 ccaccaattc cacgtgcaga accttgtggg tagggacaac caggaaacca ggtaatccca
     2941 aaaaagttcg tatcgtttct tccagattgt gactgtccat aagctgattc atagcctaat
     3001 tgagaaagaa tcacactcgg taataactga tactttacag ctgcaccaac tgctgcttgc
     3061 gcatgttcat ctgtgattgg accacgtcct ttaatctctg aaaaaatcgg taatccttcg
     3121 gtatattccc cattaaaagc gacagctaga cctgtagctt ctcctccatg ctcagaaagt
     3181 tctcctgaaa aaaagaatgg caatagaaaa aagggaagaa taaagaaaag aagacaacct
     3241 agacaccctt tcttcatttc ttcctcatcc tttcttcgat aatggttttt ggactgttcg
     3301 tccttttggt aaagtaaaat cagttcctgg ttttgcgtcc aaaatcggca gtgttttagg
     3361 atccttttct attttttccg attgctctgt tggcttatct aaagaagaaa atgcaggttg
     3421 taacgatttt ggtgcggaaa tcgacggttt actttcagac gaacgttgcc ccggttgttg
     3481 cacagaacga tttcctgata gagtggctac cgacgaatta ggaacagagt attggttcaa
     3541 taactgctgc attgctggat aactcccatt tttcgcctga tttttcactt ggcgaatctg
     3601 ttgtgaaaga gaaccagcag ctgttaattg tggcacgggt acttttccag aaggcaacgg
     3661 aatcgattga ccaacaaaag cgataccttt atttaacaat ggttttcttt gattattccc
     3721 ttgttgctta ctcaattgtg ccaatgattg tacctgttgt tttggtctta ctgaattcat
     3781 ggacgaattt ggaatcaaac catttgaaag accagaattc cactctgttt tcgatttggt
     3841 agaagacatt tcaggctcta ccccatgata acgaacatga cgaccactat aaggcaaggc
     3901 ttgatttgaa cgcccaccta gttcaaaggg attaaccact tgcctccctt ttaccgattc
     3961 ttgtggtgta cgtttttgtt gaaacttttg aaatccatgt atcgctgcag aagcgagtgg
     4021 attcgttgct cgatacacat tccctgctaa tcctgcacct ttgattgcca aattttgtcc
     4081 agcagttgat agttgaccag gcagttgttt catttgagac aaagaatgac ttgctatgtt
     4141 tttcgtgttg tttatagttt gatctgcagc tcctaaccct agtttcttta ataaatcttt
     4201 ccgttttaca atgagtaaaa ttgataggag tgctttgaca aacatatttc ctaaaaaacc
     4261 aaaaacagta ttcattggaa ttaaatcgtc tactatgtta tagatcaaaa atgccaccaa
     4321 aataatcaca ctgtaaaaag atttttgaaa catcaagccc aaaaaggact ttaaggtatt
     4381 catgagcata taatcaaata ctggaacaaa agaagcgagt aatgaaaaag gaatcacaag
     4441 taataaacac aacaccaaca tttgaaagat aaaacgaatc atgccaatca ctacaagtgg
     4501 taatccaata cttagattaa gcaatggcac catagaacca acgactgctt tataaacagc
     4561 tttagttggt tgaatataga cacgataagc cttctttttc cctctatctt ctcctgcagc
     4621 agtggtcact tttgtctcaa tatcagataa ttcttcactt gacgcttcct tggctagaaa
     4681 ttcttgtggt tcaatctctg cttgatacaa ttcttctaaa tttgttgtcc cataatttaa
     4741 caatatatac ggatcaacaa cagccgcttt ataataaagt tggcgcactt tttgaatggc
     4801 ttccttttgt gtcgttggca ctaaaacacc attgacatct tttgtcgcca attcatctag
     4861 actatttgta gcagaaaaaa taaagccttc tacctccacg gacaaatcat ttaacccttt
     4921 ggtaaaagct tctcctttac taggtacgtt actaccatag ccaaaccaaa ccattgaaaa
     4981 aatgacaacg aagagaaatc gaataaacac catttttgcg taatgtttcc ctctaaatat
     5041 acgaagagta aatagatata acacatacaa gacgacaatc cccgcaccaa taggatcgta
     5101 tatttttgaa taaatagtat ccgaaaaagt gaaaaactgg tgaattaact cgttcattgc
     5161 agctccttca tagagcttct caatgacaaa atcaaggcct tgccaaatca atttacttaa
     5221 actaaagaac atattcgtca tcatttttag aaaatctgtg ccatttgccc caaataacat
     5281 tttgcctaat gaaaattcac tatcagataa ataagaagaa aaggtatcaa tatgaaattt
     5341 cccaatctca tagatcgatt gatccacctg cttttctgcc gcatacacca atccaccaac
     5401 aaaaagcagt ccgaatccaa tcgttaaaaa aaacaaaacg caccatttct tttccttatg
     5461 ctttatataa gccattgttt cacctcattt ctattgcatg attaacgtat cctgttcttc
     5521 tacttgagag tgacttgttt tttccgttgt ttgtaatgca gcggtcattt cttcaaacaa
     5581 actgtgtaca ttgagttttc ctacacgtcc atagggatct aacatccaac actgtccttt
     5641 aatcatgtgg ctcatttcag ctaagttcgc ttcatttact tccaaacgta aatgattcaa
     5701 aatcttttct cgctcactgt cttcatcaaa agcaaagacg acaccaaaat tccctttttc
     5761 tttatctgaa cctaagtcac caataaattg tgtatctaac actaattgat tcatctggga
     5821 acgccccaca cgtgccattg ctgcaataat actacgtcct gcgttcgaat tattaaatac
     5881 ccatgcttct gtaaaataaa tcgctgtttt cttactacta tctcgttttc caaacatttc
     5941 acaaaaacga cctaaactaa tcatcaaaca caaagacttt cgatccgcat ctgtatatag
     6001 ttctggtcgg acattgtcat ttggtaattt caaatcttgg atctctaaaa tcgttgtttt
     6061 ggcattaaag tctaatcccg ggttttctcc ataagaaaat cctaaacgta aaatcgaatc
     6121 ttctaccgtc aagcgtaaaa actcaccata ttctcgaacc tctttttgtt ccatttgttc
     6181 taaatagtcg accacatgca tcaagccaac ttgttgtccg gcttcttttt gaactaaaac
     6241 ttcttggatt ccctttaata ccgccccttt taaaaggtga ttggtaccta acggacttaa
     6301 ctcatcaatc atatcctggg ctacttgttt cgcatctact cgggataaac aaacaattgg
     6361 atctaataca ccataattac ggggatttgt agcatctaac gtgacataac tcattttttg
     6421 caaatgttct acaaacaaag ggtaatgttt ctgataatac gggtcttgaa tgagtttcgt
     6481 aaaccatttg cgcttttctc ctttgggatc cacaaataaa ctttgtacat tcataaacga
     6541 actatacaaa aagagcaagc caactaaaaa ggtcttccct ttaccggttt ttccagttac
     6601 tgctacatga ggcgcatcaa acaacgcacc tttgattcct tccgcaatgg ctagaagatt
     6661 caaaaagaca aaacgatttg acgcataaat taattcttct aatggtgttt tttctcctcg
     6721 agaaccacca tcccctaatg tatcttgccg accaatatac cagccggatt ccatacctaa
     6781 ttctgatact gctgaaaaca accattcgcc aactgcttct acagttgttc ggtgcttcca
     6841 atgtaactgc cgtcccaatg gttgccctaa taaacattta tagaacaatt ctcgttgatc
     6901 attttttgca cggtggatat ggataatcgt atgaagttgt tgaataaccg ttgcactatt
     6961 ttgacgaact tcttcaaccg tttgaccgta tacaataaaa catgcgatcc aatcgacgac
     7021 aggttgccca ttatctactc gttgctttaa atgttttact aatcctcgaa cagttaataa
     7081 gcgttcacta ggttcttcat cattttgaac caattcctta tgctctgttt tgaaccgttc
     7141 cgatagccaa ttcagtttta aaccaatacc tccctgtcct ttcaagcgtg gatagtgaat
     7201 tttcattcgc aattctgctg ggaaagaaag atcctgagca aattgaaata gatcacaatt
     7261 tctcatattt tcaggtgttt ccccaatagg caacaatgct aaataactac tccctaactc
     7321 atcttctaat tgaagaaggc ctttatgttc cttcggtata cgtacatacg ttccttgatt
     7381 gtctttcagt acttcccaat tattttgttc ttctactttg tgagccattc ctcgaatata
     7441 ttgcagacga ttgagataca acagctcttt ttttactaag ggacgaatag aaaagctata
     7501 aagtaattgt gtcacttctt ttacataagg atcactttca gaaaacaaag tttccaaatc
     7561 atctacttct aatccaaata agcgtgctaa acttcctgct aaatctaacc agattagatt
     7621 tattacatca tgaaaactct ccaccgttgc tttttctttg actttgattc ctaaaataaa
     7681 tgaaggacga tagacttgac cttctcgttc caacaatgtc actgtttctt ttgcataata
     7741 cgcaccaatt gcttgactat cgggagaaaa tccctttcct aatgtgtcaa acttcgtccg
     7801 taaattcatt gttcgtggat acatcaccaa ctccacatct tcataatctg ctaaaagcaa
     7861 cagtaaacga gccatcattt cttttatttc ctgtgtcttt tttggtgtgt tttttgaaat
     7921 tgttcgtgga aaaacatgat agtaggccca gatacaatcc tctccattta acaaacaatt
     7981 tttcttgaat gcttttactg gttcttttac aagcaccaat gaatttcacc tcccaataaa
     8041 aaaggaacaa ccagttgatt gtcccttttt ctaatcctct aaataaacat acttattcac
     8101 aaaaagtaat tcttccaatg cattgtttga taccttgtcc cagttataat acacacaatc
     8161 accattcatc atacgaatca tataacaaaa ccaatcccat aaataaaaat gcactttttt
     8221 tccatcaggt tttagttttg ccagtgctaa cacacataaa taggggagtc ctatataaaa
     8281 aacaactcct gctggtggaa aaaaagaaat gacccaacca ataaatgaac gtaaaaataa
     8341 atattcaaag attacgacca acaaaaaagt tgctgcattg actaatgcaa ctggaaatgg
     8401 aagacgccaa ttgcttgtaa tctgttgtac gcgataaggt tccgcataaa caatcgaata
     8461 atcataaggt tcccaatcca cttcactatc ctcctaatcc ataaataatt tttcaatgat
     8521 tttaccaaac gcatcaaata cacgtgttgg atcatttaag aagaaataaa ctgcggcacc
     8581 tgctgcgata gatattaatg catttcctac ttttgatttc ataaaatttc gcaatcccag
     8641 ataaacaacc accattatta aaaaatagcc accttgcgtt aatataaaat ctacaattcc
     8701 cttcagtgtc atttccgttc ttctcctttc ctatccaacc aattatgtgt taatttttca
     8761 acataatact gatttgtttt tttcgtaatg acaatcgtca tttgttcttt ttgtaaaagt
     8821 gacgtatcac catctcgaaa gacgaccgtt gcttttacaa taaaaggttt tttcgtttct
     8881 ttataacgca atactttcat ctctacttga tcaactttag ctgtttgatt gagtccttca
     8941 ggttctttca tcatgtatga catatctttt gcattgtttt tcgcatactt atctaagaaa
     9001 tcttccacaa atccttcaac atctttcgct tccgacgcat caaccgtttc gtataatgtg
     9061 tcatcataag taatcattcg ttgtttttct gtattctttg gtagtgccgt aaaataagga
     9121 taagcaataa tacgacagcc cttgtttgaa atagaaaagg gaagattgag tacttgtgtg
     9181 attgtcacac ttgtttcttt cttactctct ggattcatcg agcgaatatc ataagttact
     9241 ttataactag cagttggaat ggtttcaatt tcttttaatc caataaacga agcagcagtt
     9301 aattttcgaa cacctgaaac acttcctggt tcttgaataa agttttgtgc ataaaactct
     9361 ttttttaatg tttcttgacg ttgttgttct tgttcatgat tatcgaatgt attcatatac
     9421 aaagcaacaa aacgattcat aaaggattca atttcaggtg tgtacgttaa ttctgaccga
     9481 ctttgtactt ctttcttttg taattgttgg atttcatctt tcaaaaacga cacttgaacc
     9541 ttcgttttca tggattgaaa aaatgccaag ccccctgaaa aacaaagaaa taaaaccaaa
     9601 caccaaatcc atttcactgc tttttttgaa tggattttct tttgcttcgg tactttctct
     9661 gtataaactt tttctttccg ccgtagttgt cgttttttca taagctactc cttctagtat
     9721 gtttttacta gtacgtacga ttgacaacga taagcttgtt tttctgaaaa atagtccatt
     9781 caattaccct cattttctat tccttttctc tttcttttat atccaacaaa tccagcaata
     9841 cttgtcaaca ctcctcctag aacagaaagt gcaacatgta tttcttcacc agtctttggt
     9901 aagacttttg tttttccatc tgctgttttg atttcaatat tgccacttgg aagtttcttt
     9961 acaggaatcg acaaatcctt taccaaattc ccttcactat cttggattaa tggaatacca
    10021 tcttttacac caacaattgt ttgatcacct gctactttta atggggtggt tggtgttgga
    10081 atatgggtca attcctctac attactagtg acaatcggta cactcattcc ttgagaagta
    10141 ttcgtcgtaa tttcaccgct tggggttacc gatactgtga cttcttttgc tgaaacaata
    10201 ggtttatttt cttttggaat tgcttctgat ttttcaacag gttgtttcgg ttcttctttt
    10261 tgttcttcac tggtagacga catcgttggc tgttccgtac ttgacgaatc agtattcttt
    10321 tcagaagaac tggttgttcc tgattgttcc ggagttgtga ctatacttga atccgttgtt
    10381 gttgaactat gcacaccttc ttcttcgatc gaatcagtcg gtaaagttga ttcaggaaac
    10441 tctggttctg gtgtcactgg ctctttttca ggtttctctg actgcaattg ttcaggttct
    10501 gctggttttt ccgttgttcc tccatttagc tctgtctcaa catctggtaa tccttcccct
    10561 ggcaatgttt ctacaatttc tgacttaggt gtttctacag tttcatctgc ataaaccaat
    10621 gaactcccta attgaaacga aaataatgaa attcctagtg ttgttacgac tgctaatgtt
    10681 gtttttttca tgattattcc tccaattttt tatgcaattc gtgttttgtt catagacgcc
    10741 gcactaatat tatctgaacg ataaaagaac tcccgtccgt tttcttctaa cccaacatat
    10801 ggattatttt tgaataattg aatcaatagt tcactggtgt tgtattttgg atagtttgca
    10861 aaaaaataat actgtgcttg tcgcccatcc gaaaattcaa caagtaattc ataacgatac
    10921 cgcttcaatc cttttcgttg ttttaaagga tctctatacc aacttgtcca tcgttccatt
    10981 cgtggataga gatagacacg atacacatag aacaatgcca taccacatac caccaaaaat
    11041 aataatcgcc acataaaaat tccccctttt cttctacctt tcttgaatca tcgaacattc
    11101 ataccctcta tagagatttg aaaaatccat catcaattgt tcatataaac gttgttgcaa
    11161 atagcgattg tttcgaatac ataattcttc atagccatca atcacttgat cttctttttc
    11221 atagcatctt gtagctctat gacgctcagc ttgcaagaca taaccaaact ctgcaccata
    11281 tcgttctact gttaataact gttgactacg caataatgca atataacaat cacaaaaatg
    11341 ttcaaccgac atttgatttt tctcacgttt gtgaaatagg tattctttag aaagcacatt
    11401 attcgttaaa caatctgata aatagcgata atctggcggt cggttatttt catctgtgaa
    11461 tttttcccat aataaactga tttttattcg ttcagcggct agatagtctt gttcgcgtgg
    11521 ataaaattca tcaatcgcac aaagtttttt atcacctgaa taaacaaatc catataatgc
    11581 gaaacgaact cttccttttc aattggctct cctaaaaatt ccagtgacat accatcttct
    11641 ccttcctaaa aaaaataaaa aagcagaaac cgatttggct tctacttttc tttcctttta
    11701 tagctctttt ttacggaaga atccgcaaat gacatttttt caaaaaatca atcggcacaa
    11761 gttcacgaaa acttggagca attttggata gaatcacttc cccatcttgt aaaatacaat
    11821 cataaaaatc atcagttact tgatacttac gcttcttaca ataattgata aaatagtagc
    11881 gatcattttc tatttccatt cgttgttcta ctaagcgcat acgaacatca tcatagaaat
    11941 aagccacaaa tcgcccttca agttcatgtt tttcaatggg atactcttct ggtaacaatc
    12001 tattttttcg tcgtttatac attgctggat accttagaag aagttcatct aaacgtctgt
    12061 cagtatatgg taactgacga atcaacgatt ctaatcgttc tttatacaga tagaataatt
    12121 ggcgcaagca atagtgtacc gcctctggat tttctaatac tgttggatca cgtgaccaat
    12181 acgcaccaac ataaatcata atttttttca tttagacaat cacaccagca atactaatga
    12241 ccgatccaca ataggtagaa caagtggcat agcgaccaat ttcctctaat tctatctcaa
    12301 tctcttctaa agaaagatat gtctgttctt ctgttggctg gcgactcatt tcccaagtga
    12361 cagtcaccaa tgaaatttct tccataagag gtttttcgct gctaagtgct gccacacagt
    12421 tttcatatat cttcatttta tcgcagcctt ttttaaccgc taaaatcgca ggtattggca
    12481 atgaaaatcg ataaaagcga atgaattcta gccttccttt ccttcggcta aattccaact
    12541 cttcgagttc ttgttcgtat cccttacgaa cttctaataa gcgtgcaatt tcttgccagt
    12601 tctctttttc ctgataacgt ccagctttta ccgcttgttc aatataagga actcgaaaac
    12661 gaaaataaga caaatcaacc atcttttcat ttaatgcttc gattgcagcc tttaaatcca
    12721 acaggcgcag ccattctgtt gtcgtatatg cattcatgca attttccctc ctgacgttcc
    12781 catccattct agatagtttt gttgctcgtc atttagttcg gcattatcaa tcccttcttg
    12841 tacaaagtct cttcctaaaa tacgatctgc ttcttgtaac aactttaatg tcggagctac
    12901 ttgtttcaaa aaccagcgtt ctgaacgatc taatgagaat ttttctggct ttactgtaaa
    12961 atccaattct tccatcgaac gaaataattt tgcccatcgt ttatcaacaa tccgtgcatc
    13021 actttccagt ctatcccaaa caataagttt tgcattaatt aaaccaacgc caatctctcc
    13081 taaacgtttc ggtgaatcac ctaaaataaa tgcttctact gcttgtgttg ctttctcatt
    13141 ggctaaccgt aactcatatc gattataaat accatatttc gttaatgctt ctaattctga
    13201 aattttttct cggttcgcaa tttcaaaacg tttctcataa aaattaaaaa agagaggact
    13261 ctttcgcgat ccaaaataca aagataatcc ttcacttcga aaatagccat catctaaaaa
    13321 acaaccgcca atattctgga atctcttaaa gtccatttct aataaacctg ctttttgttt
    13381 ttctagtaat tcaaagagat cgaaatgttc ttccgtttcc aatccccaaa gttcatctaa
    13441 cgcaatatcg aatcggctac attgcacacg tgtataacca gcttccaact ttccttgatt
    13501 tttccaaatg gaatatagcc atgttcgcca atctgtttgt tgttcacgca tgacttcctc
    13561 atattcccgg caaccttgtc ctgaaaattg cacaagtaca ttttctgtta cagttcgttt
    13621 tgaatgccag aacacccaga tattcccacg aacagaaaga cagtcaaaaa gatacaagcc
    13681 cgttggacgt gtttcaaatt ctgtcagatc cattcgtaat acacgttgaa tcactgtttt
    13741 gtagtctgtc ccaaaaaagg aaagacgcaa ataatcaatc attgctcgaa gatctattcc
    13801 gcttttcata tacaatcgaa agcgttcctt gattcgttgt tcaaaatcct ctgggatttt
    13861 tcttttccct tgttccatct ttctaatgta atcttttgag cagcccatca attgcgcaaa
    13921 tcttgcttgt gatagcggct ttttatctcc acttatatta tgaattgcac ttaacttctt
    13981 tctgtagctt tttagctcaa tcggtgtcat tttctcgctc cttttataaa ataacaaaat
    14041 cgattttttt aaccctttag aaacaccgat atatcaacta gtccccctcg ttttttggga
    14101 aaagtagtac aactgctgac cttagataat cacttgatac aaaagggttt ataaaacttt
    14161 tttctatgtg ttatgttatt tctgcccccc atattatata tcggggggct ttacacaaag
    14221 aggccgtgcg caagcgcacg acctctttgt gagctgcaca cgctgctagc gagtgcgcat
    14281 tataggccca tgcttcgcag ccttaatgcg caccgccagg cagagtgcgg ctaccagact
    14341 acgtctagag ccctcccgat agataataac tacaaaatta aatgatctaa ttttgttatt
    14401 tcactatatt tttcaggaga aaaaacgcga taaattgaca aagttaaatc ctcttctaaa
    14461 tctactcgat aatgatacaa tcgagtcaat cgttctatat cttttttcgt tagcaatact
    14521 tgattaataa cttttcctgt aaaaacacgt tctttgcgca aaaagcggta atcagttact
    14581 tgttcaatac gattcaacca atcataaaac gttctggaag aaagagaagg attaatcatt
    14641 cgtattgctg tagctactct ataataacaa ctctctgcca tttaacttca cctccttagt
    14701 tgattacaaa atatgcgcat cagaaaaact tgttcctttt ggatcttttg ctaaatacat
    14761 ttggcgctct agttgttctt gtaacaactg attatcatct cccatcgttt gatcttgact
    14821 atcctctttt actgtaaaat catagacaat catttcagta aatttctcaa attctttgat
    14881 aaagtgataa tctttgtaaa caaatggcgc aaaaaactct acaggtacat caaatcctgg
    14941 aaaagcatat ccacgaccag gttgatcttt gttgtaaaat ggcttatttt taaaatcttc
    15001 tccaaaagtc attaaataac caatgggaga caatcgacca acagaaatac ggaatgttaa
    15061 attatcccga acaccacttg gaaaatattc tgcatctggt cgttgcattg ctaaaataat
    15121 gtacatccct gcttgacgac cctttaatac taatggctga atcaatttat cgactctatt
    15181 tctcgcttcc atatccaacg aaccataaaa agctgcccat tcatcaaaaa agataaaatg
    15241 cggtggtaac ccataataag cataattttt ccctggttca tatttaggat ctttcatggt
    15301 tttcatatag gcataccgtt gattcatttc ttttaatgca tttcgtaaac aaataatcat
    15361 ttcttcattt attttgtaaa acacatgtcc tttgaataca tcgacgtcac ctaatgctgc
    15421 taaatctgcc gcttttccat cgcaaatatc tactgtccca acacttaaac aagcttgaat
    15481 taaagaatac aataaatagg ttttcccacc accgattgca ccagcaatca acatatttgg
    15541 tgattgatca tatacccaat ccaaagtttt cattaaatga atacgtccat tttctgctat
    15601 acattgatca ataggaatcc gaccagaatc tgaaaggaat acataattga ttcgggcttt
    15661 ttctcgttct tcacgaatta aatcacgcaa aaatacttca gataacaaat gacccatttt
    15721 taaaaatcgt tcgtgaaaac gcccaccatc taaaggaaaa gaaaccacta attcttgttt
    15781 cttttgataa aaataaactt ttggtaatct cattttttcc cttgtttgtt tctcactaac
    15841 ttgtttcttt gtataaacaa accgattgtc aattaacatt cgaacaagta actgacgcca
    15901 ataaacagag gcaaaatatc cttcttttag tcgcgaccaa tgcatcaacc ctaaaattaa
    15961 cccactaacc agcaacaaat acaacacaat acttattcca ggaataagat acatttgcca
    16021 attcgaaaac gtacttaaac gtggataaaa tactttccaa ctaaaccaac ctaacagaat
    16081 caaaaatgga aacaaaaaga aaccataata gcaaaaccat aaataacgat tgaaatagcg
    16141 aattcgccga ccacgatact tgaaaaaatg agctttcaat tgtcacattc ctcctttctg
    16201 tttttacttt tcctgtgcta aaaacttttg acaaaaagca actaatacag gaatatcaca
    16261 tttagtaaaa aaacataatc gaaaaagatt taatcgattt ttataagggt gtgcacacat
    16321 attacatatt cgtactatac gaatccaaat cacttcctga tgatcttgtt tccactggta
    16381 cggtaatgaa aaacgaacaa gatcacgtaa aaacatacac tgcccaataa ccgcacaaat
    16441 atatgcattt gtttgtacaa aaatcgttgg attgtctcca aaatactcaa tcaattgttg
    16501 gatattttga taacaagcac aatctctcaa agtatttgat tggttttatc tgaattcaaa
    16561 atctcccctt ccttctatct tagataaaaa gaataggaag actccttcct attctttcaa
    16621 aaaaacctta ttttttctct ttttccttag aatcaacact tggttgtttt tgatctaaac
    16681 ctttttcttg tgattgtttc atattaggat tatttgttgg agcttctact ttctctaatc
    16741 ctttcgcata aatcactaaa tctgaatatt cacgattgcc atcttgtgct ggaatatata
    16801 ccacatgata ttctggatca attaatttca caggatcacg tcgtttaaaa ccagtcaaat
    16861 ctacatctgc tggaaattta actcgtcgtg caatctttgg ttcatctgga tttccaaaaa
    16921 ctaattgtgc tcgctgtgat cgaatttctc ccgtataatc gcctgtttca ttatcaaata
    16981 cacgatcaga ctcttctaat gcgccaaagc gtacggctcc taaacaagtt tcatctaatg
    17041 cttctgtaat gtcataatta tttggaaatt ttaatcccat cttacaaaac ctccccaatt
    17101 tttacaccag ttaaaaagac accaacatct tgatagccag ttgttcgacg ctctgtttgt
    17161 cccatatcat ttgtttcacg actattcact cgttctttac gaaaaacaaa ataagcttct
    17221 tcaaatgaaa tacgcacctc tttttctggt aatcgtaatc ttttatcctc ttcagctgtg
    17281 agataaacaa aaaaagcagg ctgttcttct cgcgaacact ctgcttgtaa ttgaacatat
    17341 cgcccagaca caccattaat ttcctttggt ggattaggtt cccgtgaacc caataaaaat
    17401 acttgaccta aaaactcttt cttcatacgc aatgcttttt ctgccataaa gtttttcctc
    17461 ctcttaaaaa ccaaaataaa ttctaaatat ttctacatta caatcaaaca agcggttaaa
    17521 aatcctaacc gcttgttcct catgactaat ctttggcttt acgtacttta taaagtgtta
    17581 tacctaaagc aatgactaat actactaatc caagaaccat aagtatacta cttgtttgtt
    17641 cacctgtttt tggcaacatt ggtacaacag tagctggttt aacccgttgt tctggtttca
    17701 ctggttgcgg tggttttggc gcttccacaa tttgaaccgt ttgatctttg tttgttagat
    17761 ctacattgtg tttcgccact tcttcaaacg tttctttctc atcttccgtt ttcgcttgga
    17821 gccattccgc aaaactcaag ccttttcctg caagattgcg accgtccact aagaattcaa
    17881 ataagaattc catcatttct ttatcggccg caaagacttt ttccgcatga gtgccttcaa
    17941 atggtgtgcc gtctggtgtt aagtagcctt gaacaattaa gcgatagatt tgaccaatcg
    18001 caagaccgtc tactttgacc cattcaagca cagtttgatt tggcaaggcc aagatctctt
    18061 ttccgccatt tttattcgtc gctttcgtcg taatggttgg attactgatt ttgatggttt
    18121 ggtcattgtc ggtaatatct gcgtgagaag ccaataatac gccttctcta aacagatctt
    18181 caaagaccac gacttctttg cctcgcaacg cacgtgcgtc tagaataaag tctagattga
    18241 ctgtaccatc cgttgtttct gccacaaacg ttaaggtact tgtgacttct tttccatcta
    18301 ctagcactgg tttttccgtt actttgtcca ttagaacacc gttgactgtg tatgtttttc
    18361 caacgaccaa atcgtggtat ttcacagcgt ctgtaatggt gactttcgtt aatggattaa
    18421 cctctttcaa gccagtgggt ttatgtgtag ccgttgtacg tacacttggg ttagttactc
    18481 gtaccgtttg ccctttgtcg ttgatgtctt tatggtttgc tagttcttca tcatttctaa
    18541 aaagactttc aaacacgacg atttcacgac catttatacc tgtcaaatca aaggtaaatg
    18601 gtacgatcac cgtttcattt tctgctttgg caataaagcc aacactcgtt tctaccgtat
    18661 ttccatgcac aagcaagggt tcaccggttg ctttatccat taagatccct ttgaccaagt
    18721 atgcttttcc tggaagaatc tcggtaattt caacggtatc gtttaaggta aacaatcctg
    18781 ttgggtctgc gactgtcaat ccgtcttcaa agacagccgt ggtatgaacg gttggattat
    18841 tcactttcac actttgggct tcatcgttga tatccgcatg tgctgcaact tcaatgccat
    18901 cacggaaaag tttttcaaaa acaacgatcg tttgaccttt cagaacacta gcattaacca
    18961 caaattcaag gtcttttgtg ccattgactt tgtctgcaat aaaggccaag gtacttgtga
    19021 tttctttgcc atcttgtaaa attggcgcat tcgttgcctt gttcatcaat actcctgaaa
    19081 tggtgtattc ttttccgaca aacaagttgg tataagaaac actgtcttta atgacgatcg
    19141 tttctaatgg atcaaccact ttttcaccat cggctttatt tgacgcttgt gtttctagtt
    19201 ttggtggaat caagtaatta cgtacagaaa ccactaacgt ttccccatca tctggcgcat
    19261 tttgtttgaa tgcaaccgtg aatgcttgtt tatctgtatt tggttggaaa ctttctaatg
    19321 gggcattttc caagaagtaa tagtcatcgg caactagacc tttcactaaa atttccccgt
    19381 tttgatcagt ggtatattca ccaattgctt gatttttacc gttatacaag gtaaagttca
    19441 cacctgcgag cggcgttttc ttcttgttcc attcttcata cttgaataat tttacacttt
    19501 tccgtgcttg acggttcgca aaatggatca ctttgatcgg ttctgtatca ttggtaatcg
    19561 agaacactaa tgggttttga ttcaaaacgt aatttacggg ggctttgatt tcttccaaac
    19621 gatagcgatc ttttccataa gtaagtgtac cgttagttag gaaaaagcct ttttcattgg
    19681 ttttaaagat atccgttgtt tctgtatcat ttggaatctc cattgtgaca taacgattgg
    19741 ctaaagtatc aaaaatcttg aattctgtat tagcaagtgg aatcactttt cctgtttctt
    19801 gatccacttt caccacttta agacgttgtt caattacttt atcttctaaa acataatgga
    19861 aagtttgccc attttcagaa attgtgactt caagtggttc aaatagttta tacccttctt
    19921 ttcctttggt ttcagaaaga atgtacgtat caaagacaac ggtattttta ccaaaagtag
    19981 cataaccatc attgtctgtc acaacggttt tcaccacttt gccagttgtt tttgacgtca
    20041 aagtgatttc aatgccagct aattttggtt tgttatctgg attcgttgaa tcgactaaat
    20101 ctttattagc aactttcact aaatcaatgg ttcctttgat cacttgatct tttgcggtcg
    20161 cattgacagt ttttccaggt tcaacaacaa tttcaattgc ttcatcagaa agcacataac
    20221 cttttggcgc tttgatttct tttaaaacat atttgccagg atcaagatct ttaatttctg
    20281 cctgtacttt gccgttaatt tttttcaagg taatttcttt aatctttttc ccatctgctt
    20341 gatacaaacc atagaccgca ttttctaaag aagctgcacc ttgtgctttc acaccagttt
    20401 ccgcatcttc tttaaacaca cgaatgtttc cttgttttag aatttgtaaa ttaataacag
    20461 acgcattagg ttctgttgga tgtaacacac caattgtttg agctcctgga cgacgatagg
    20521 ctaatggcgc accttgaact ttaattttat tcaattgaat ttttgcttta tccggtgcct
    20581 cttttgtgac tgtaatccat acttcattgc cgtgaataga tacattgaca ccttttggtt
    20641 tttgaatgtc tttcacatat ttcaaaaaat catttttatt ctttactttc aacgtttcgc
    20701 caatttttac cgtatgtgtt tgattattaa aagttgcttt ttcaccaaag ttattaatca
    20761 aacgttgaat ttctgctttt cgacgatcat aatcaacacg gatatttttg attgggattt
    20821 ttgtgcccaa agcttcccaa atcataaatt gtgccgcaat gtattgttca tcggatttgt
    20881 ctttatttgt gacatatcct aaataagaga tcaagcttgt ttttttccgt acatttgaat
    20941 ttttcaaata gtttttcaaa gcagtagctg tataaacttg accttctgcg gctgagacac
    21001 caggctcaat acaaaacgcc acttgtccat taatctcaaa acgaaccata taatttaaag
    21061 tgaagtttag attgttcgga ctagaagtaa caccaatatt ttcgccccaa tcaattttcc
    21121 gaccacctaa aacagctgca ttggttgcac ggcgaacacg aaccgaacga ttggttggta
    21181 ctgttactaa cagtgtaggt gtacctgttt ttggaatgtt ctgctctttt ttcttttgat
    21241 tttcaatact agccgtagat tctttgtttg gtttcgttgg ttgattttga ctagctgcgg
    21301 ttgaagagat tgttttttca acgttttcct cattttttcc ttctttgtca gcagtagccg
    21361 ctggttgttc cactgtttct tctattcgtt tcttttcttc cgcttgtttc tcagaagact
    21421 ctggctgcac agaaggcgtt gcttcggcag ctttttctcc ttctagagtg gacgtttctg
    21481 aagcaggggt tgtaattgag gcagtttctt caactggttg tgaagtacca ggactattcc
    21541 ctgggtgttg tgtttcccca atcgtctgat cgagagtcgt tttggaattg accgttggcg
    21601 cttctgtcac aggggcctgt tcaacagtgg cttcaccagc tgggaccccg gcttctgctg
    21661 gttggacttc tgtcacttca gaagcgactt gggcaatggc ttccatcgta ggttgtatag
    21721 ccgtcgtttc ttctgcatgt gcttgagcag ttcccaccaa gaaacagaat tgagaaaaag
    21781 ccaccaatgc gagtgctttc aaccaattca ttttcgtttt tgcatacaca agaattcctc
    21841 cttttcctga gaattttata taataaaaca aaacgccgtt accaaaagtg ataacgacgt
    21901 gaactgtctc ttctattttt atctggcgct cttatctgaa cacatttcat caatgatttt
    21961 accctcccac tataattaca gaacaaaaaa agaacctgct tcgtggaaat caactgtcca
    22021 tttcccaatt ggaataccag cttttttggc aacttcacca tcaccaaatc tcatgccata
    22081 ccaattacta ccttcatcct taatttcttt ccaaccatat gacatagctt catctaatga
    22141 atcaaaaatc atgacactat tgccaattgt tacaggatct ggcttaagat cttctggaag
    22201 agaaatatca atcataccat aatgatccga ttcctcagta ttattgtcat tactagctac
    22261 tggtttggtt gttctactgg cttattttga tacaacaagt actcttattc ttcttttact
    22321 tctccgtttt caatttccca ttcaaaccac tccgcaaagt cttgcttaaa accatctact
    22381 cgatacctat tttgtaaata ggcatcttct gacgtcgtag gatctaaata ggtaggttta
    22441 ttctctggac gagcatagac acctacccat gtttcgtaat ctccttcaaa ataactatct
    22501 accaaatagc ctttttttct aagaatctcc cgaacttgat ttttaattat tgatacttgt
    22561 tcaaaaccat cactcattct atcacctcaa ataactctag attctcataa atattcccga
    22621 caatttcaat cctatcaatg atctctaaca aatcgctact tatgcttccc cattcgtata
    22681 agaatttccc ttccgcaaaa taaattttcc ataaacttct tcatgatcgt cccaaccaat
    22741 atcactctcg aaaaattcca cgttgttctt atcttttagc tcagtaaatg ccataagttc
    22801 aacatgtttt ccaacctcga aacaatcaca ccacccatca accgttacac gtgtgccttc
    22861 aatacctaag aaactttgtc ctcgtgtatt aaacactaaa actttccgca ttttttgctc
    22921 attaattaat cacgcgcgaa atctaggaat cacaacttat tcgcttcttc tttataaata
    22981 aaaaaactcc aataatcgga aattactatc ttttcctcat tcctttttta aatcttcata
    23041 cttcttctta tctttgtcct aacctaataa cacaaatttt gaagaaaaac tcagtaaaac
    23101 ctgattcttt ttttatcttc acaaca
//
LOCUS       DAYYCW010000040        22996 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000040 DAYYCW010000000
VERSION     DAYYCW010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.040"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..421
                     /locus_tag="ACHAYA_001400"
     CDS             191..421
                     /locus_tag="ACHAYA_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM8605203.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            518..1819
                     /gene="murA"
                     /locus_tag="ACHAYA_001401"
     CDS             518..1819
                     /gene="murA"
                     /locus_tag="ACHAYA_001401"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8605204.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1832..2008
                     /locus_tag="ACHAYA_001402"
     CDS             1832..2008
                     /locus_tag="ACHAYA_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8605205.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2143..2439
                     /gene="yidD"
                     /locus_tag="ACHAYA_001403"
     CDS             2143..2439
                     /gene="yidD"
                     /locus_tag="ACHAYA_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM8605206.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2624..3091
                     /locus_tag="ACHAYA_001404"
     CDS             2624..3091
                     /locus_tag="ACHAYA_001404"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8605207.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3176..4060
                     /locus_tag="ACHAYA_001405"
     CDS             3176..4060
                     /locus_tag="ACHAYA_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8605208.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4174..4410
                     /locus_tag="ACHAYA_001406"
     CDS             4174..4410
                     /locus_tag="ACHAYA_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM8605209.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4498..4578
                     /locus_tag="ACHAYA_001407"
     tRNA            4498..4578
                     /locus_tag="ACHAYA_001407"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4532..4534,aa:Tyr,seq:gta)
     gene            4585..4656
                     /locus_tag="ACHAYA_001408"
     tRNA            4585..4656
                     /locus_tag="ACHAYA_001408"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4617..4619,aa:Gln,seq:ttg)
     gene            4781..4951
                     /locus_tag="ACHAYA_001409"
     CDS             4781..4951
                     /locus_tag="ACHAYA_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605210.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5171..5878
                     /locus_tag="ACHAYA_001410"
     CDS             5171..5878
                     /locus_tag="ACHAYA_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8605211.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5937..6581)
                     /locus_tag="ACHAYA_001411"
     CDS             complement(5937..6581)
                     /locus_tag="ACHAYA_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM8605212.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6707..6994
                     /locus_tag="ACHAYA_001412"
     CDS             6707..6994
                     /locus_tag="ACHAYA_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605213.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7102..7422
                     /locus_tag="ACHAYA_001413"
     CDS             7102..7422
                     /locus_tag="ACHAYA_001413"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8605214.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7503..8816
                     /locus_tag="ACHAYA_001414"
     CDS             7503..8816
                     /locus_tag="ACHAYA_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8605215.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8813..9532
                     /locus_tag="ACHAYA_001415"
     CDS             8813..9532
                     /locus_tag="ACHAYA_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFM8605216.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9660..10211
                     /gene="raiA"
                     /locus_tag="ACHAYA_001416"
     CDS             9660..10211
                     /gene="raiA"
                     /locus_tag="ACHAYA_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM8605217.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10433..12967
                     /gene="secA"
                     /locus_tag="ACHAYA_001417"
     CDS             10433..12967
                     /gene="secA"
                     /locus_tag="ACHAYA_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM8605218.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13093..14194
                     /gene="prfB"
                     /locus_tag="ACHAYA_001418"
     CDS             join(13093..13164,13166..14194)
                     /gene="prfB"
                     /locus_tag="ACHAYA_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM8605219.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14299..14985
                     /gene="ftsE"
                     /locus_tag="ACHAYA_001419"
     CDS             14299..14985
                     /gene="ftsE"
                     /locus_tag="ACHAYA_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM8605220.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14978..15862
                     /gene="ftsX"
                     /locus_tag="ACHAYA_001420"
     CDS             14978..15862
                     /gene="ftsX"
                     /locus_tag="ACHAYA_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM8605221.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            16014..17462
                     /gene="cls"
                     /locus_tag="ACHAYA_001421"
     CDS             16014..17462
                     /gene="cls"
                     /locus_tag="ACHAYA_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8605222.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17799..18656
                     /locus_tag="ACHAYA_001422"
     CDS             17799..18656
                     /locus_tag="ACHAYA_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM8605223.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18771..19691
                     /gene="pstC"
                     /locus_tag="ACHAYA_001423"
     CDS             18771..19691
                     /gene="pstC"
                     /locus_tag="ACHAYA_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM8605224.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19691..20575
                     /gene="pstA"
                     /locus_tag="ACHAYA_001424"
     CDS             19691..20575
                     /gene="pstA"
                     /locus_tag="ACHAYA_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM8605225.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20590..21396
                     /gene="pstB"
                     /locus_tag="ACHAYA_001425"
     CDS             20590..21396
                     /gene="pstB"
                     /locus_tag="ACHAYA_001425"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8605226.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21412..22170
                     /gene="pstB"
                     /locus_tag="ACHAYA_001426"
     CDS             21412..22170
                     /gene="pstB"
                     /locus_tag="ACHAYA_001426"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8605227.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22183..22860
                     /gene="phoU"
                     /locus_tag="ACHAYA_001427"
     CDS             22183..22860
                     /gene="phoU"
                     /locus_tag="ACHAYA_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM8605228.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
BASE COUNT         7579 a         3917 c         5024 g         6476 t
ORIGIN      
        1 ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt tgcatatttt
       61 tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat acttgttttt
      121 ccttttatac attttatggt attatcattt tattagttat tataaaacaa tcgttagaaa
      181 ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga gtcatttggc
      241 gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt ttttcagacc
      301 aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt tagggtacac
      361 tgcaagtaat tttttcttag aagttatcgc tttatgccga aatcttttcg ttagcctgta
      421 aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa gattttgagt
      481 aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca tcgtaagagg
      541 tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg ttttgcctat
      601 tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg ttccgattct
      661 ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg tagattttga
      721 tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg aagcgcctta
      781 tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac ttgccagaaa
      841 tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac caatcgattt
      901 gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg gctatatcga
      961 agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta gtgtaggtgc
     1021 tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca ttgaaaacgt
     1081 agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg gcgctcaagt
     1141 ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac atgcagtcaa
     1201 ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg ctgcaatgac
     1261 acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat tgatttctaa
     1321 attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg tcatcggacc
     1381 aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc ctacagatat
     1441 gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtag tgactgaaac
     1501 agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg ctcatgtcaa
     1561 aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg cagaagtata
     1621 tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag caaatggaat
     1681 cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc atatcaaact
     1741 acagcaatta ggtgcagacg ttgaacgtgt agacatggat caaacttctg ctgaaaaaac
     1801 agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata tcttattaac
     1861 tttgataaag attttaatgg tgatcttatt gcttatcctt ctttttgtgg ccggaacgat
     1921 gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtatttcagc caagtttgtg
     1981 gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc tagtcgatat
     2041 gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt gacgttagtc
     2101 gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa gatattgatt
     2161 tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag ctgccgttat
     2221 tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggcgc gctaaaaggg
     2281 acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg cggtatcgat
     2341 tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga accagattat
     2401 aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaacaattt ggaaaatagc
     2461 ttcttatatt ttgcaaaatt gtgagttatt gccgaagtgt tggattttga acaccgtcaa
     2521 aaaaaaggtt gtgaaaaata cggctagcac tgagcgataa agaaaaagag gaaatttatt
     2581 gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg aagaactaaa
     2641 caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag aaaaagttca
     2701 agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa caataggtgt
     2761 actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag taaccccaac
     2821 gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga agctttcaca
     2881 actcgcttcc agtcaaactc agatcatcat acgaaccggc agtactagca gtgcggcgtt
     2941 acatctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact attttatcca
     3001 tcattactct gagcccattt ttgaaaatgg agatcaatta agggatcaag tgattttagc
     3061 aattcactta cctaaaaata gctttaacta ataaaatttc aattcaagaa aatgctttca
     3121 aatgctaaat agatacatta caatagcttt agcaaacaaa aaggagaata tttttatggg
     3181 aatattagtt tttgattttg gtggatcggc tgtcaaatat ggttgttggg atggcaaaga
     3241 gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga aagcgcaatt
     3301 atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg gaatcagctc
     3361 tcctggagta gtgaataatg agaaacaggt gattgaggga atcagtgcga ttccttatat
     3421 tcatggattt aatattttcc gagatttgga aactctgttt caacttccag taacgattga
     3481 aaatgacgcc aactgtgccg gaatggctga attttatgaa ggtgctgccc gcgattatca
     3541 agatgttgct tttgttgtgg caggaactgg gattggcggg gcgttgttca cgaaaggaca
     3601 aatcaataaa ggggcacatc tttacggtgg agaatttggg ttgatgttct taaatggcga
     3661 taagacattc agtaaacttg gtacagcggt acagatggct tggcgttatt gtgaacgcaa
     3721 aggtctcgat aaaaatgctt ataccggaaa agatgtcttt gaattagcag aaactggaga
     3781 tgcaatagca aaagaagaag ttgagagttt ttatacgtat cttacaaagg gattattcag
     3841 tattcaattt tcgtttgatc cagaagtgat cgtattaggc ggcggggttt ctgcaaaaga
     3901 agggctcatt gatgagatca aaagccgaat gaagaagctg acagaacaat tcgatctgca
     3961 tgatttcgaa ccgcagattt tattatgtga atacagaaac gatgctaatc ttgtaggtgc
     4021 agcagcaaac tatctcgctg tacgtcaagg ggagaattaa gagctttaga gaaggtgcag
     4081 cttgcataaa agaagtgaat gaaatgaata ggagattttt acctaaggaa tgttttattt
     4141 gctgcagtat gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa taaagatatt
     4201 gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta agaattatga agtcactcag
     4261 ttatttatag ggaaaaaaat gattggtgag atcttagcct atggaccaaa agaatatgaa
     4321 attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc gatagaaaca
     4381 gtgattcgac actggaatct gcacgaataa aaaaatatca aaaaaacttt caataaaggg
     4441 ttgcaaaaat ctggaaaatt aggtattata actaagtcag gttgatacaa acctttcgga
     4501 ggagtagcga agtggctaaa cgcgacggac tgtaaatccg ttccttaggg ttcagtggtt
     4561 cgaatccact ctcctccatt tcattggggt atagccaagc ggtaaggcaa gggactttga
     4621 ctccctcatg cgttggttcg aatccagcta ccccagtata ccacaatcgt aagctctcgt
     4681 tagttcgaga gctctttttt ttgctttgct accttgttcc ttgaagattc tgctgattta
     4741 agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga aagaaaaagt
     4801 agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca atattgaagt
     4861 gaatattccg aaaaaaggcg gaaaagcggt ccgggtaaaa tcaaaacgcc caacgaatca
     4921 tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata agtcggcatt
     4981 ttcatcgatt ttagggtctt ttgttatttt ctaggttgga catatctaaa aaattgtttc
     5041 tgacaatagg agtaggaaga acgatgtgtg tctaaatcct ctgttgatgg atagaaacct
     5101 ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa cgaaaggacg
     5161 ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc aattaaaaaa
     5221 tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa gagaattagc
     5281 agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact tggcagatga
     5341 aggaatctta gaaagaaaga tcggttcggg tacatatgtt gcacgagaaa aagtacaaga
     5401 aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc gagtaccgtc
     5461 tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg aaaaactaaa
     5521 cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag atgatgtccc
     5581 tatttgtttt gaagttgcca gtgtgccaca aaagctgatc gatggctaca gcaaagcaga
     5641 gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga tcggtccagc
     5701 gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt tagaggtaaa
     5761 acgaggagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg gtacgccgtt
     5821 tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg aaaaataaaa
     5881 aacaggtgat ccctgattcc gtattagaaa taaaagagaa tcagaatcac ctgtttttat
     5941 atatgatcag ttaccagttg ctcgtgataa gaaacaggac cctcttcaaa ttggacttgt
     6001 ccgttcagca gctcaatgat ggattcttta aagtggtcag cacttttttc atctacagca
     6061 cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg ttggagttca
     6121 agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat ctcctgttgc
     6181 aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg agtgtatgct
     6241 cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac tactgcagct
     6301 acattgatca gttgattttt tttgaggact tccaacatcg gtatgcccgc cgttccactt
     6361 ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat gaaggcgctg
     6421 cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaact ttttgcttcg
     6481 tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat ctcgatctca
     6541 ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc tcttttttca
     6601 ttttctttat tcagtataaa taaagttcta ggttgtttca acactgtaac ggttgcgcaa
     6661 ttgctgtagt atgcgataat aaagacaaag gcaggtggat ctggggatgc aggaacgaat
     6721 gaaaaagtac gatgcaatta cgcattattt gaagaacaat ggcggctctc aagtaacgtt
     6781 gacctttacc caatttgacg aactgctgtt tcccagtaac ggacttccca aaacagcaag
     6841 ggaaagtaca gattggtggg caaatgatta taaacaccct gaaaaaggtg cctatggctg
     6901 gataaatgca ggctatgaag tagttgtcat caatttggac aaagaatatg tcgtgttcaa
     6961 taaattagtg aaatctagtt ggctatttga ttaaataaac cgcgtttaga aaacgttgac
     7021 aaatttcttt ctttttatat aatagagtaa cagacgcgaa gaaaacgttt gcgtagtttg
     7081 ttttaaaaag gagagaaaag tatggctaaa aaaacaatca tgcttgtatg ttcggctggt
     7141 atgagcacaa gtttactagt aacaaaaatg caaaaagctg ctgaagcaaa aggaatcgac
     7201 tctgatattt tcgctgtgtc cgcttctgac gcagataaca atcttgcaaa taaagacgtg
     7261 gatgtgttgt tattaggccc acaagttcgc tttatgaaag ctgattttga gaaacgtctg
     7321 gaaccaaaag gcattccttt agatgtcatc aatatggctg attatggtat gatgaatggc
     7381 gaaaaagtgt tagatcaagc aatcacgctc ataggtgaat aaatagaaca gtataactat
     7441 cgaacaagcg atccaaaaaa tagatcgctt gttttttcgt atattttttt agggcgaata
     7501 atatgaatca tttttggggt agaaggcttc tgacgagaga tattaaagca atagactgtg
     7561 aggaggtaaa tttaccaaat tatgagaaga tcgcagcagt ggaacgtttg agcaactgga
     7621 ttctttgtca tcgctgtgga atgagaacgt ccgtcattga tggacagctt gcagattacg
     7681 gttatttttg tacacattgt ctatatttag ggcgatgcga cactcaacaa gatttatatt
     7741 tgtttgatca gcctgaagca aaacctcgag aagtcgtctt tttttggaca ggacagctta
     7801 ctgaaaaaca aacggaaatc tcaaagagaa tactttgcca ttcagaaaaa agacatcatc
     7861 ttatttgggc tgtaacaggt gcagggaaaa cagagatgct ttatcctatt ttagtggaaa
     7921 ctttaaaagc tggaggaaga gtagcgattt gtacaccgcg agtcgatgtt tgtaatgaat
     7981 tgtttttacg ctatcgttat gtttttccag aagaaaaaat cacgttgctc catgggaata
     8041 cagcaacggc ttataccttt acttcttttg ttatctgtac gattcatcag ctataccgtt
     8101 tttatcggtc ctttgattta atagtgatcg atgagatcga tgcgtttcct tataacgggg
     8161 atgcaggatt agctcatgct gtgcaaacag cgattaaacc agacggacga ttcatctact
     8221 taagtgccac acctgataaa aagttactaa aggagatcaa gcagacgttt gacatccaca
     8281 aacttgcttt acgctttcat cggcgtttgc ttcctgaacc cgtcttggtt ttcatgaata
     8341 attggcgaca aaaatgcaag aacaagaaga agataaaatc tttggtgcgg atcattcatc
     8401 aattaattaa agaaaaccat atccttttat tttgtccaag tatcacgatg atgcatcgtt
     8461 tgaaagccac acttcaagag gctcttccga gatatgagat cacagatgtt tcttcaagag
     8521 acaatcagcg ggcggaaaaa gtccagaaaa tgcgagaagc aaaatatgac atactgttaa
     8581 ccacgatgat ccttgaacgt ggagtcacat ttgaaagaat atccgttatc gttttaggag
     8641 cggatcatcc ggtttttact aaatcttctt tggtacaaat cgcaggaaga gccgatcgta
     8701 aagggcctta tacgaacagt caagtatatt ttttctatga agaaaaaact gcagctattc
     8761 gaaaagcttg tcaggaaatc aaagaaatga atgaagaagg gaagaagata ctgtgaggtg
     8821 cagttgctgc agtaaagata tcgtccaaaa tttaacattc agcgagatta ttggtgtttc
     8881 gaaatacaga ttacctcaga cttgttcagt ttgtcgcgca caatttcatt tattggaaac
     8941 aagaggctgt tctggctgtc aaaaaccaat ccatttcaat ctagagagta cgaaaaagaa
     9001 agtaaattct atccccgttt actgcagtga ctgtcaagaa tggaagaaga aatatccgac
     9061 ctatgatttt cagcaccgtg ccttttttgt gtacgatgaa aatatgaagg cctggctcaa
     9121 tcaatacaag tttatgggag atatccgact agcaggcacc tttgcatata aatggacgca
     9181 gataaaaaaa gaatacccga catacatcta ttgtccaata cctttatctg aaaaaaggcg
     9241 aacagaaaga ggattcaatc aagtaagcga gatgttgaaa gcagcaagaa ttccttttca
     9301 gatgctactc aaaagagaga agcatctgct ggctcaagca aaaaacacgc gaaaggaaag
     9361 attggagatg cctcaacctt ttgaacttgc agtcaccttt tctaagatac aaaataaaaa
     9421 aatattgatt attgatgatg tctatacaac aggacagact cttttccacg cagcagattg
     9481 tttgcagatc gcacagccga aaatcatccg aactatttct ttggcgagat gaattagatg
     9541 tggaaatttg atagcgattt aatttgcaaa taacctagag ttcgatataa tagaattaag
     9601 aagagggaaa gagtggtcct tctccttctt gattgtggta gacgaaaggg gtaattttta
     9661 tgtttagata taatgtacgc ggcgaaaata tcgaagtaac tgaagcgatt cgcgactacg
     9721 ttgagaaaaa agtaggcaaa ctagaacgct atttcagtga cgcacccgat gcaacggcgc
     9781 atgtgaactt aaaagtttat acagaaaaaa cagcaaaagt ggaagtaacg atcccattac
     9841 cttatcttgt attacgtgcc gaagaaactt ctccagattt atacgcaagt atcgatttgg
     9901 ttgttgacaa attggaacgc caaatccgca aattcaaaac aaaaattaat cgtaaatccc
     9961 gtgaaacggg catgaatact gcaaatgctg ctgctttatt caatgaagag gacgaaaacg
    10021 aaaatgattc tgaattagat atcgtacgta cgaaacgcct ttcattgaaa ccaatggata
    10081 gtgaagaagc agtcttgcaa atgaacatgc tgggacataa cttctttatt tttgaagatg
    10141 cagaaacaaa tggtacaagt atcgtttacc gccgtaaaga tggaaaatac ggactgattg
    10201 aaacagactg atcagcctta cttaaataac aacgaaaact tgcagcagct actgacagct
    10261 gccgcaagtt tttttgagtt tcctcctctt ttagatgaaa tatgaataaa ttatgaagtt
    10321 cttactttaa tacgcttaag atatttcaat tatctataac taatgataaa atgaaaagta
    10381 aagtctacat agtctggata atacacagaa ggaaaaggag attacacaac gcatggccaa
    10441 tttgattcgt tcacttatcg aaaacgataa gaaagaacta aaacgtttag atagcattgc
    10501 aaaaaaagtt gaagcatacg ctgaccaaat ggcagcatta actgatgaac aattacaggc
    10561 aaaaactgct gaattcaagg gcagatacca aaaaggagaa acattagatc aattacttcc
    10621 ggaagctttt gcagtcgtcc gagaagcagc aaaacgtgta ttaggtttat atccatacca
    10681 cgtacaattg atgggtggaa tcgtgcttca cgatggaaat atccctgaaa tgcgtactgg
    10741 tgaaggtaaa accttgaccg caacgatgcc agtatatcta aatgctctga ctggcgaagg
    10801 ggtacacgta gttaccgtca atgaatattt agcaacccgt gactcgacgg aaatgggcga
    10861 actttataat ttcttaggat taacagtggg gttgaatatc aactcaaaat cttcggatga
    10921 aaaacgcgag gcttataatt gtgatatcac ttatagtacg aataatgaat taggatttga
    10981 ctacttgaga gacaatatgg tcgtttaccg ccatcaaatg gtacaacgtc cgctgaatta
    11041 tgccattgtc gatgaagttg actcaatctt gattgatgaa gcgagaacgc cgctgatcat
    11101 ttcagggcaa gcagaaaaat ccacagcttt atatacaaga gcagacaact tcgtgaaacg
    11161 tttgaaagaa gaagaagatt ataaaatcga tgtacaatca aaaacaatca gtttaaccga
    11221 agcagggatc gaaaaagcag aagaaacatt cggtttggat aacttatacg acattgaaaa
    11281 tactgcattg acccatcact tggatcaagc attgagagcc aactatatca tgcttttaga
    11341 tatcgattat gttgtccaag acggaaaagt actgattgtt gaccagttca ctggtcggat
    11401 catggatgga cgccgttatt cagatggact tcaccaagca atcgaagcta aagaaggcgt
    11461 agagatcgaa gacgaaacga aaaccatggc gacgatcact ttccagaact atttccgtat
    11521 gtacaagaaa cttgcgggaa tgactggtac agctaaaaca gaagaagaag aattcagaga
    11581 aatttataac atccaagtat accagattcc gacaaatcgt ccggttatcc gtgatgatcg
    11641 tccagatttg ctttacccaa cattacaaag caagttccac gctgtcgtgc aagatatcaa
    11701 agatcgctac cgcaaaggtc aacctgtgtt agtcggaact gttgcggttg aaacatctga
    11761 gttattatca gatatgctga atcaagaacg tattcctcat gaagtgctga atgcgaaaaa
    11821 tcactttaaa gaagcagaga tcatcatgaa tgctggtcaa aaaggtgctg tgacgattgc
    11881 taccaacatg gctggtcggg gaacagatat caagttagga cttggggtaa gagaacttgg
    11941 tggtctagca gttatcggga cagaacgaca tgaatctcgt cgtatcgata accagcttcg
    12001 aggacgtgca ggacgtcaag gagaccctgg tatgtcacag ttctacttgt cattggaaga
    12061 tgatttgatg aaacgattcg gttcagaacg aatcaaatct ttcttagatc ggatgaagat
    12121 cggtgaggaa gatgctgtta tccaaagcaa gatgctgacg aaacaagtag aatctgcaca
    12181 aaaacgtgta gaaggaaata actatgacac acgtaaaaac gtcttacaat acgatgatgt
    12241 gatgcgtgaa caacgtgaag taatctataa acagcgacaa gaagtaatca tggaagaaaa
    12301 aagcttatcc aagccactaa tgaacatggt caaacggaca atcagtcgtg tagtagatgc
    12361 gcatacacaa ttagagaaag aaaattggaa tttggaaagt atcatcgatt ttgccggcag
    12421 tactcttgtc catgaagata gtattactct tgcagatatc gaaggaaaaa cagctcagga
    12481 gatcaaagag tatctaaacg aacgagcaca agaagtattt aataccaaag cggctcaact
    12541 aaatggtcca gagcaactgt tggaatttga aaaagtcgtg atcttgcgtg ttgtggactc
    12601 taaatggaca gaccatattg atgcaatgga tcaattacgt cagtcaatcg gcttacgtgc
    12661 ttatggacag aacaatccat tagtcgaata tcaaacagaa ggctataaga tgtttgaaga
    12721 tatggtaggc gccatcgagt atgaagtcac tcgaatcttt atgaaagctg agatccgtca
    12781 aaacgtacag cgtgaacagg tttctcaagg aacagcttct cactcagaag acggagatgc
    12841 caaagaaaat agtaatacta gcgcgaaaaa acagccaatc catgtgaaca aggtcggaag
    12901 aaatgatcct tgtccttgtg gaagcgggaa aaaatataag aactgtcatg gaaaaaatgc
    12961 aaactaattt ctgacagcag gattggtgag acaaattctt tttttaagaa ttcgtctcac
    13021 cttctttctg ttatttgctg attctgttag actagatata atgaaaaaag tatagtgaaa
    13081 gaaggaaaaa acatggaaat atccgaaatt cgaaatctcc tgacagaaat gcaggaaaag
    13141 gtcacaagct tcaggaggtc tctttgactt agaccagctg gaagaagaca ttgcggaagc
    13201 agaagcaaaa atggctgaac ctggtttttg ggatgatagc gaagctgctc aaaaagtgat
    13261 cagtgaaaac aatgcaaata aagaaactta tgatcaattc cattctcttg ctgaagaact
    13321 ggaagaaatc gaattgatgg tggagatgtt gcaggaagaa cctgatgcag ccatgcaaga
    13381 agaagcagaa acacgtatca atgcgctaga tgaaaaaatg aagacatatg aattatctat
    13441 gttgttagat ggtccttatg atcgaaacaa cgccatcgtt gaattgcatc caggggctgg
    13501 cggaacggag tcccaagatt ggggcagtat gcttttacgt atgtatacac gctgggcaga
    13561 gcagcatggg tatcaagtag agacactaga ttatcaagca ggtgatgaag caggaatcaa
    13621 gagtgtgacc ctactgatca aaggacacaa cgcatttggt tatctgaaat cagaaaaagg
    13681 tgttcatcgc cttgtacgta tctcgccttt tgactcagcg aaacgacgtc atacttcttt
    13741 ttgttcggta gatgtgatgc cagagcttga tgaaacgatc gatatagaaa tcaatccaga
    13801 tgatttgaaa atcgatacgt accgagcaag tggtgcaggt ggtcagcaca tcaataaaac
    13861 agaatctgct gtacggatca ctcacttgcc tacaggaaca gtagtagcta gtcaggcaca
    13921 acgttcacaa ttgaaaaacc gtgagcaagc gatggggatg ttaaaagcta agctttatca
    13981 attagaagtg gaaaagaaag aacaagaagc agctgcactt cgaggagaac aaaaagaaat
    14041 cggctggggt tcacagatcc gttcttatgt tttccatcca tattctatgg taaaagacca
    14101 tcggacaaac gtagagacag gaaatgtcca agcagttatg gacggtgacc ttgatttatt
    14161 tattgacggc tatttaaaaa tgcatctaca ataagaaccc attgttaaat tgaaataaaa
    14221 ttgtaagatt ttgcaagcat gttgaaacat tgccatgcta taatagctaa agactgaaaa
    14281 agatatggag agaacgccat gattgaaatg aaggatgtaa tgaagaagta ctcaaatggt
    14341 acgaccgcta tccgcaatct ctcaattgtc atcgaacaag gagagtttgt ctatgtggtt
    14401 ggtccgtctg gagccggtaa atctacattc atcaaattga tgtatcgaga agaaaaagct
    14461 tcaaaaggaa ctttgaacgt cgcaggtcat gatctaatag caatcaaaaa ccgcgaagtt
    14521 ccttatctac gcagagaaat cggcgtggtg ttccaagact ataaattatt accaagaaaa
    14581 acagtatatg aaaatgttgc gtacgctatg caagttatcg gcagaaaacc tcgggatatc
    14641 aaaaaacgag taatggaagt gctggacctt gttggtttga aacacaaagt tcgagtcttt
    14701 cctagcgaat tatccggtgg ggaacagcaa cgtgtctcta ttgctcgggc aatagtgaat
    14761 acgcctaaag tacttatcgc ggatgaaccg acaggaaatc tggatccaga aaactcatgg
    14821 gaaatcatga aattattaga tcggatcaat gcccaaggaa ctactgtcgt gatggctaca
    14881 cataacagta cgatcgtaaa tacgattcgg caccgagtaa ttgctatcga aaacggccgc
    14941 attattcgag atcaagcgga aggggaatat ggatacgatg attagaacct ttttccgcca
    15001 tttattagaa agtatcaaga gtttgaaacg taacggctgg atgacagttg cttcagtcag
    15061 tgcggtaacg atcacattga ctttagtcgg tgtattcatg gcagttatca tgaacgcaac
    15121 taaattagct caagatatag aaggaaacgt tgatgtctct gtattcgtag atatcggtac
    15181 aaaagacgaa gagatgaaaa agctagaaaa agaattgaat gacttagatc atgttaaaaa
    15241 agttgaattt tcaagcaaag aacaggaatt gaaaaaaatc caaaacgaaa tgggtgattc
    15301 ctggaaattg tttgaaggtg acaataaccc gttatatgat gtgtatgtag taagtgctac
    15361 tgatccttct tatacaaaac aagtagcaga agaagcagca gatttgaaga atgtttctcg
    15421 ttctgattac ggtggagctt cctcagatcg gattttccaa atcgctggtg cagttcggac
    15481 atggggatta gttgcagcgg cattgctgct gttcgtagcg atgtttctca tttcgaacac
    15541 gattcggatc acgattttgt ctagacagcg tgaaattcaa atcatgcgtt tagtcggtgc
    15601 aaaaaatgga tatattcgct ggccgttttt ccttgaaggt ggctggatcg gtctgatcgg
    15661 tgcgattctt ccgattttga tcatcacatt tggttatgca tggtttttcc aattgatcaa
    15721 tccatcatta ttacgttccc attattcatt gattcatcca caaaatatcg tttggaaaat
    15781 caatctttta atggtcggca ttggtgtgat cattggttca cttggctcga tcatttctat
    15841 gagacgtttc ttgaaattct aataccacaa tataaataaa atgagaaagt tggacagttt
    15901 cgttccagct ttcttttttt attggctagg agaatagaag gaagcggctt ttttcccttt
    15961 actttaaaaa acaacttggg tattcttaag ttggtagaag aagaggtgaa gccgtgaaaa
    16021 ttatcgttga caattttttt acgatccttt tgattatgaa tattttgctt tcctttatca
    16081 tcgtttttcg agaaagaaag gaaactgctc aaacatgggc atgggtgctc gttttgatgt
    16141 tcattccagt tgtaggattt attttatata tttttttagg aagagggatt tccaaagaga
    16201 aaatctttga tttgaagatc caagggaaaa tcggtaagaa cctggaaatt gaagaagaca
    16261 aacaggcatt gatgcgaggg ctttatcctc atccgcctac tggacaagta gatgtgaaac
    16321 aattgatcta tatgctcacg gtatttgaaa gtacgctcta tacgactaaa aatgaaatca
    16381 ctttatttac agatggtcgt gaaaaattcg atgcgctgat tgaagatatc aagcaggcag
    16441 aagaccatat ccacttccag tattatattt atcgcagtga tgcattaggt gaagaagttc
    16501 gagatgcatt gacagaggcg gcaagacgag gggtaaaagt cagagtcctt cttgatgcat
    16561 ggggttccac acaagtttct tccagctttt ttcagaattt gaaaaaagca ggcggagaag
    16621 tcgcattttt ctttcctcta tttgttccgt atattaatcc tcggatcaat tatcgcaatc
    16681 atcgtaaaat cgtagttatt gatgggaaaa tcggatatac aggtgggttc aatgttggaa
    16741 atgaatatct aggattggta aagaaatttg gttattggcg agacaatcat ttgcgtattt
    16801 acggagaagc ggtgtatacc ttgcaaaacc gttttttgat ggactggaat tctcagcata
    16861 caaaagaagt aggctattct cccaaatttt ttccgtccat tcactctact ggtgatatag
    16921 ctgttcaaat cgtgacaagc ggaccagata ccgaacatga acaaatcaaa atgacttatt
    16981 tgaaaatgat ttcgttagcc aaacgggaga tattgatcca aacgccttat tacataccgg
    17041 atggatcgat tcatgaagca ctgaagttgg ctcttctttc aggcgtaaaa gttcatatcc
    17101 aaatacctaa caaaccagat catcttctcg tttactgggc aacgtattct ttcgctgcag
    17161 agctaatcga gtatggagca aggatcgaaa catatgagaa tggatttatt catgcaaaga
    17221 cgatgattat cgatggtggc attgtttcag ttggctcagc aaatatcgat gttcgttcat
    17281 ttcgcttgga ctttgaggtc aatacactgg tttatgatga acgaatggct tcacgtgtaa
    17341 gaaaagcttt ttttgaagac tcgaaaattt ctacgcatct cacaaaagaa atgtatgaaa
    17401 accgaggaat aatcatcaag atgaaagaag gattagcaag attgatttcg ccgctccttt
    17461 aaaacataaa aaggacatga gaaccatcaa cagatggaga tctcgtgtcc ttttctaacg
    17521 gggccatttc tgagagtacg tcagaaatgg agaaagcgtc tcgcatgagc gaaattaact
    17581 ttggggtatc tcttgttttt tggggaaagc aagaaataag ctaatatatg ctattaaaag
    17641 agacacattt caaagccagt atagcataga aacttttaat gagatagttt tttttacaat
    17701 tatgagaatt tattttgtta tttaatgatt ttgattcgtt ttcatcaagt aatgaaaatg
    17761 ataaaataaa catactttag gattaaagga gatttctaat gaaaaaactt aaattattct
    17821 taccaataat aggattagct attcttttat caggctgttc aaagtggatt gatcgaggag
    17881 aatccattac ggctgttggc tcttctgctt tacagccttt agtagaaaca gttgctgaaa
    17941 cttaccagac agaaaatcct ggaaaattca tcaatgtaca agggggcgga agcggaactg
    18001 gtttgtctca agtacagtca ggagcagtcg acatcggaaa ctctgatttg tttgcggaag
    18061 aaaaatcggg gatcgatgcc tcagcattag tcgatcatcg agtagcagtt gttgggatca
    18121 ctcctattgt gaacaaggat attggcgtaa aagacatcag tatggaaaac ctgaaaaaac
    18181 tatttacagg cgagatcaaa aactggagtg aagtcggtgg aaaagatcag ccagtagtta
    18241 tattgaaccg cgcttcagga agcggaaccc gtagcacgtt tgaaaaatgg gtattggacg
    18301 gagaaacagc tgttcaggca caagaacaag attcaagcgg aatggtgcgc cagatcgttg
    18361 ccgacacacc cggtgcaatc agctatgttg ctttttctta tgtttccgat gatgtagata
    18421 cgttgaatgt taacggtgta gaaccgacag atgaaaatgt gacaacgaac aaatggacga
    18481 tttgggcata tgagcatatg tacacaagag atcatccaaa aaaactgaca aaagaatttt
    18541 tagactatat cttatctgat gaagtacaag ataacatcgt tggagaacta ggttatatcc
    18601 ctgtttctca tatgaaagtc gaacgtgatt ggcaaggaaa tatcgttaaa gagtaatctt
    18661 tacacaaaat ttacaaactt agctaaactt ttacatgtta ttaaaaccaa attgatgttc
    18721 aatttgtaca ctgagtgagt atgttaaacc tgattaaggg ggagtcatct gtggaagatg
    18781 tgaaaaaagt attattaaca aaatcaaaac gttcaaaaat ggaacagcgt ggaaaattca
    18841 tcagtttttt atgtatcgcg ctgatcgtgt tggttgtatt atctattttt tattttgtag
    18901 ctagtaaagg attggccacc ttttttactg ataagatcaa tgtatttgat tttctgtttg
    18961 gtacagattg gaatccaagc caaatcggag caaatgggaa accaatggta ggggcattgc
    19021 ctatgatcac aggttcgttt attgtgacct ttttatctgc tatcgttgcc acgccatttg
    19081 ccattggtgc agcagtattc atgacggaga tctctcctaa aaacggaacg aagatcttac
    19141 agccggtcat cgagctgctt gtaggtattc cttctgtcgt ttatggtttt atcggtttat
    19201 cagttatcgt gccttttgtc agaagtattt ttggcgggac tgggtttggt atcttagctg
    19261 gtacatttgt gttgttcgtt atgatcttgc cgactatcac aacgatgact gttgatgcat
    19321 taaaggcagt gccacggcac tatcgggaag cttcattagc gcttggtgcc acaagatggc
    19381 aaacaatcta caaagtcgtg ctacgagcag ccgtgcccgg gattttaaca gcagtcgttt
    19441 ttggtatggc tcgtgcgttt ggtgaagcct tagctatcca aatggtcatc ggtaatgcag
    19501 cattgatgcc aacaggtctt actactcctg cttctacatt gacgtccatc ttaacaatgg
    19561 gaatcggaaa tacaatcatg ggaacagttg aaaataatgt gttatggtca ttagcgctga
    19621 tcttgctatt gatgtcatta ttctttaata tcgtgatccg tatgattggg aagaaaggag
    19681 agctgaaata atgaacgcaa aacgctcaga taaaattgct acaggtgttt tatatatagt
    19741 ttcggggatc atcgtattga ttttagcagc tttactactt tatatcttag ttcggggaat
    19801 accgcacatt tcatggtcct tcttgaccga accttccaaa gcctaccaag aaggcggtgg
    19861 aatcgggatc cagctattta actcgattta tttgcttttg atcacgatga tcatcagttt
    19921 tcctatttct ttaggtgcgg gtatctatct ttcagaatat gctggaaaaa actggctaac
    19981 agatgtgatt cgcacgtcga ttgaaatctt gagctcgttg ccttctgtcg ttgtcggttt
    20041 attcggtttc ttggttttcg ttattcaatt ccagtatggt ttctcgatta tttctggtgc
    20101 attggctttg actgtattca acttgcctct tttgactaga acagttgagg agtctttaca
    20161 agcggtccat tacacacaac gagaagcagg attggcctta ggtttgtcac gctgggaaac
    20221 agtgataaaa gtggtggttc cagaagcgac accagggatt ttgactggtg tgatcctaag
    20281 ttctggaaga atttttggtg aagcagctgc attgatctat acggctggac aaagtgcacc
    20341 tgcgttagat tttacgaact ggaatccatt aagtgtttca agcccgatca gcattttccg
    20401 tcaagctgaa acattagctg tccatatttg gaagatcaat acagaaggca cgatgcctga
    20461 tggtgtttct gtttcggctg gcgcttctgc cgtgttgatt attgctgttt tactgttcaa
    20521 cttcggcgct cgtgcgattg gcaaacgatt ataccgaaaa atgacttcag cctaaggaga
    20581 agaagcaaaa tgaaaaaata taactggaat gaaacaaatg tcatcaccat gccaaaagag
    20641 aaagaagtag cactatatac ggatgatcta catgtctggt atggcgacaa tgaagcaatc
    20701 aaaggagtgg atctggaatt tgaaaaaaat aagatcactg cactgattgg tccttccggc
    20761 tgcggaaaat ctacgtatct tcgttcgttg aatcgaatga atgatgggat cgctaatacg
    20821 aaaatcactg ggaaaatcat gtatcaaggc gtagatatta atgcgcctca agtggatgtt
    20881 tatgagatgc gcaaacggat tggcatggtt tttcaacgtc ctaatccgtt cagcaagtcg
    20941 atttatgaca atattacatt tgctttgaaa cagcacggcg aaaaaaacaa aaagaaatta
    21001 gatgaaatcg ttgaaacaag cttgaaacaa gctgccttgt gggaccaagt aaaagacagc
    21061 ttggacaaaa gtgccttggc tttatctggc gggcaacagc aacgtttatg tattgctcgt
    21121 gcgatcgcga tgaaacctga tatcttgctg ctagacgagc cggcaagtgc tttggaccct
    21181 atttcgacag gaacggttga agaaacactt gtaaacttga aagaagacta tacgatcatc
    21241 atcgtgacgc ataacatgca acaagcagct cgtatcagcg actatactgc ctttttctat
    21301 ttaggaaaag taatggaata tgacaaaact aacaagatat ttaccagacc aaaaattcag
    21361 gcaaccgaag attatgtgtc cggtcatttt ggttaagacg gaggcctcaa catgacaaaa
    21421 gagattattt catctaaaga tcttcatctg tattatggaa agaaagaagc actaaaaggg
    21481 atcgatctaa gcttcaatca aggggaaatc actgcgatga tcggtccttc tggttgcgga
    21541 aaatccactt atctgcgttc tttgaaccgg atgaatgacc tgattccgaa tgtaacgatc
    21601 accggaagtg ttttatataa agacaaagac atctatggtc caaaaacaga tgttgttgaa
    21661 ctaagaaaag aaatcggcat ggtattccag cagccaaacc catttccttt ttctatctat
    21721 gaaaatgtca tctatggatt gaaattaaaa ggggaaaaag ataaaaaagt ccttgatcaa
    21781 gtagttgaag aaagcctgaa ggcagcgtct gtttgggatg atgtgaaaga caaattgcat
    21841 aaaagtgctt tatctttatc cggtgggcag cagcagcgtg tatgtatcgc acgtgttttg
    21901 gcagttaatc cggaaatcat cttattggat gaaccaacaa gtgcgcttga tcctgtttct
    21961 actgggaaaa tcgaaagtat gctgttagaa ttaagagagc ggtatacgat gatcatcgtg
    22021 acccataata tgtctcaagc ttcacgtatt tcagataaga cggcattctt tttagatggt
    22081 cacttgatcg aatacaatga tacgaagaag atattcatga atccagaaaa acaagagaca
    22141 gaagattata tttcaggtcg atttggataa ggaggaaacg gaatgctacg tacacaattt
    22201 gaagaagaat tgctgaatct ccataatcag ttctacgaaa tggggatgat ggtgagcagc
    22261 gccgtgcaca agtcagttcg tgcatacatc aaacacgata aaatcttagc ccaagaagtt
    22321 atcgaaaacg atataaatat caataacatg gaaacacgct tggagaagaa aagttttgaa
    22381 atgatcgcat tgcaacagcc agttacaact gatttgcgta tgatcattac ggtcatgaaa
    22441 gctagctcgg atttagaacg tatggccgat catgcggtgt ctgttgctaa atccacgatt
    22501 cgcgtaaaag ggcagacgag aataccggaa atcgaaaaag agatctccga tatgtccgat
    22561 tatgtgaaaa aaatggtgga caacgtccta gttgcttatg tcaaaacaga cgaaaaagac
    22621 gcacgtacga tcgccaatat ggatcaaaga gtcaatgact atttcaatcg aatctataat
    22681 gcaacaatca aaaatatgca ggaaaatcct gagacagtca tcagtggaac agactacttg
    22741 aatgttgcca gttatctaga acggatcgga gactatgtga caaacatctg tgaatggatc
    22801 gtctacttgg ctacagggaa aatcactgag ttaaatacga accacaatga gattttctaa
    22861 actgaagctg tgattatgcc gtttgtttag gaaaagaggt tgtgaaatca gcggttgtga
    22921 aaaagctgtt ctgcatcaag taataagaac agtcaaacaa aaaatagctc ctcatatttt
    22981 tcgtttggct ggactt
//
LOCUS       DAYYCW010000041        22954 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000041 DAYYCW010000000
VERSION     DAYYCW010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.041"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHAYA_001428"
     CDS             complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHAYA_001428"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM8605229.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHAYA_001429"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHAYA_001429"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8605230.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="ACHAYA_001430"
     CDS             8118..9086
                     /locus_tag="ACHAYA_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8605231.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..10009
                     /locus_tag="ACHAYA_001431"
     CDS             9104..10009
                     /locus_tag="ACHAYA_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8605232.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10006..10812
                     /locus_tag="ACHAYA_001432"
     CDS             10006..10812
                     /locus_tag="ACHAYA_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8605233.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10969..11937
                     /locus_tag="ACHAYA_001433"
     CDS             10969..11937
                     /locus_tag="ACHAYA_001433"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM8605234.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12254..12778)
                     /locus_tag="ACHAYA_001434"
     CDS             complement(12254..12778)
                     /locus_tag="ACHAYA_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM8605235.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13065..14876
                     /gene="scm"
                     /locus_tag="ACHAYA_001435"
     CDS             13065..14876
                     /gene="scm"
                     /locus_tag="ACHAYA_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8605236.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(15031..16017)
                     /locus_tag="ACHAYA_001436"
     CDS             complement(15031..16017)
                     /locus_tag="ACHAYA_001436"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8605237.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(16040..16606)
                     /locus_tag="ACHAYA_001437"
     CDS             complement(16040..16606)
                     /locus_tag="ACHAYA_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM8605238.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16620..17036)
                     /locus_tag="ACHAYA_001438"
     CDS             complement(16620..17036)
                     /locus_tag="ACHAYA_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8605239.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17493..19427)
                     /locus_tag="ACHAYA_001439"
     CDS             complement(17493..19427)
                     /locus_tag="ACHAYA_001439"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8605240.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            19729..20664
                     /gene="pplA"
                     /locus_tag="ACHAYA_001440"
     CDS             19729..20664
                     /gene="pplA"
                     /locus_tag="ACHAYA_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFM8605241.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            20754..21857
                     /locus_tag="ACHAYA_001441"
     CDS             20754..21857
                     /locus_tag="ACHAYA_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8605242.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            21870..22823
                     /gene="menA"
                     /locus_tag="ACHAYA_001442"
     CDS             21870..22823
                     /gene="menA"
                     /locus_tag="ACHAYA_001442"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM8605243.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT         6740 a         4863 c         4488 g         6863 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttggg
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
     9601 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
     9661 cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa
     9721 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
     9781 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
     9841 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
     9901 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
     9961 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    10021 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    10081 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    10141 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    10201 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    10261 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    10321 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    10381 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    10441 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    10501 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    10561 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    10621 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    10681 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    10741 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    10801 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    10861 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    10921 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    10981 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    11041 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    11101 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    11161 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    11221 ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg agattctgtt
    11281 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    11341 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    11401 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    11461 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    11521 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    11581 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    11641 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    11701 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    11761 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    11821 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    11881 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    11941 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    12001 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    12061 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    12121 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    12181 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    12241 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    12301 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    12361 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    12421 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    12481 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    12541 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    12601 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    12661 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    12721 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    12781 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    12841 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    12901 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    12961 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    13021 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    13081 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    13141 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    13201 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    13261 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    13321 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    13381 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    13441 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    13501 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    13561 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    13621 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    13681 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    13741 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    13801 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    13861 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    13921 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    13981 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    14041 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    14101 ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat actacctcta
    14161 ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact acctctacca
    14221 caagtgatag cacggacaca tcagcttcaa gtgacagtac agatactacc tctaccacaa
    14281 gtgacagcac ggacacgtca gcttcaagcg acagtacaga tactacctct accacaagtg
    14341 atagcacgga cacatcagct tcaagtgaca gtacagatac tacctctacc acaagtgata
    14401 gcacggacac gtcagcttca agcgacagta cggatacatc taccacaaca agtgatagca
    14461 cggacacgtc agcttcaagt gacagtacag atactacctc taccacaagt gatagctcag
    14521 attcatctac cactacaagt gatagcacgg acacgtcagc ttcaagtgac agtacagata
    14581 catcctctac cacaagtgat agctcagatt catcaacttc aagcgatagt acagacacgt
    14641 cgtctaccac aagcgataca cgtcatgcaa gtgtctcctt ctcaaaaaca agttcggata
    14701 acaggacgaa ctttaactta actaataaaa caagcaatga caatggaaac aaatcaaaaa
    14761 gagcacttcc taaaactgga agtcaatcga ataattggat aacactcgca ggcgttatat
    14821 tacttgtaat cggtttaaga atcactttta tgagttataa gaaatcgaaa aggtaaaaaa
    14881 atattcaaga taaaaaagag caacgtcgct aacggacgtt gctctttatt tttgcttttt
    14941 atcataaata caagaactac aattcaattt gtcaaaaaac acgtagattt ccttctgaga
    15001 aatttattta cgtatcgaac ctttccctat tcagtctttg cgatccaaaa tcagttgggt
    15061 aagggaaaag agattctctt ttgttcgaag agaggtggca ggtaatttct ttatttcctt
    15121 caaagccttt tctgtgtaag aggccgctaa cttcctagct tcttccacgc ctcctgtatg
    15181 ttcaacgatt ttgcgtacgg cgtcgacatc ttcttctgtc attgccgctt tcttctctaa
    15241 ataaggtagt agctttggtt tgtttttttg caaagagtag atcaacggta aagaatagac
    15301 cccttgacgc atgtcttcta gtacaggttt gccgatttcc tggctctctt ttgtataatc
    15361 taaaatatca tcgataatct gaaaggccat cccgatagaa agtccgatat ctcctgcttt
    15421 ttttgcgaag cgctcgcttg ttccactttc aaaagcgcca acagaacaac ttaattggaa
    15481 cagttcagct gtcttacctg aaatattttc caaatattct tttacagaaa gttcataatt
    15541 atagcgatca tccatctgcc ctaattcgcc attcaatact ttttccatac tgcgagaatt
    15601 caattggaga cttttcaagg aagtggagta atcagacaat agtttaaaac agcagacaaa
    15661 taaatagtct cctgcataga cagaaacgac attaccaaac tgactacgga tattaggctg
    15721 accacgtcgc agatccgctt catcaacgat atcatcatgg atcagtgtgg ccgtatggag
    15781 catttcgata gaagctgcca aagcgatggc tttgtctcga tcttgctctg gtccaaattg
    15841 tgagaacaat agctgatagg caggacgcaa cagctttccg ccggcatgga tcgtttcttt
    15901 gattgcttgt tcgacaggtt tgtttttcag gcggacagtt tcttccatcc gttttagtgt
    15961 ttctgtcaat tctttactta aagcaggata gttttcccac attggatgta gcttcataaa
    16021 aaaactcctt gtgtgacgat tattttttgg taaacattgg gctccatttt tgtttggttc
    16081 cctgctcata atctttttgg aatcgttgaa gtgtctgcac atgtcgtaaa agataattcg
    16141 ctgcgatccc gattgcaatc cctgataaaa tccctaaaac tgaaaggact ggcaagtaca
    16201 acatgactgt ccatgattgt gcaatgaaag aagccatcaa aagctgtccg acattatgca
    16261 tgaaaccgcc ggccgcacta attccgatga tactgacacg ttttggtcct aattgcttga
    16321 ccaataacat tccaaaataa ctaagcaaag caccactcat actataaagg aatgtggaaa
    16381 tcgttccgcc tagcaaagta gtcaggaata gtcgtagcca aagtagtaag aagctgtctc
    16441 gttttttcat ggtaaaaatg gcaacgatcg tgattagatt ggctaagcct agttttgcac
    16501 caggcgcaaa agaaaaagga taagggatca tattttccaa taaaccaatg atcactcctt
    16561 gtgcaacaag tagcgaaata taaatcattt tttgtaattt actcatggtg tgtgaaggtc
    16621 taataaatca gactgtcttc gtcacctcca tctgttgaac ggacttcgat cactaattta
    16681 tgtggcaaac agacgatggt ttcgccattt ttcgttgccc agcctcgacg tacgcagatc
    16741 tggtcatcgc aatctgcttc ttttatccgt actcgatcgc catctatctc gatcaagtta
    16801 taatctccgt gttcatcttt gtaagtatat gtgttgctct cttctccagc catcaaaggg
    16861 aatgttttga tttctgtgcc atccacacgc agtaccgctt ctttttctgc tgttgcggtg
    16921 ttttgctgaa gaccaaaaat catgactggt aaaaaagaaa gaacagtcaa aagcaaaata
    16981 ataaaaacat cccaaggttt gagcagactt ttttcagcaa attctttaag gctcattttt
    17041 gttctccttt ttccagatta actcacccaa ttttagcata aaaacaagag gttgtgacac
    17101 ctgtgtcttg ctttgagtaa taagaacgaa gaatagaaaa cagctttcca tgttttctaa
    17161 ttctttgggc ttattaccga gaagcagcag gtggaacacc gtttataaca agaggttgtg
    17221 acagaagtgg gcaacttcaa gaaataagaa ggaatttacg aaaattactt ttcaagtttt
    17281 tgtgaaattt cggcttattt ttcgaagaag ctacttctgg aacaccgttt ataacaagag
    17341 gttgtaacag aggttgtgac acctgtgtct tgctcatgga ggcttatctc gggcatctta
    17401 ttcctttaat tttacataaa aaggaggctg tgacggtttg tctcagcccc tttctattga
    17461 aatcttgcat tcaaataacg ttctttatct atttatttct tgccgtactg ggaacttggt
    17521 ctaccgtacc accaattacc tagtgtacgt tgtagccatg gaattaccca gcgatccaac
    17581 ccaatcgcac gtccggaacc gttcatcaat gcgaaagcaa caaaaatgaa ccaaatgttt
    17641 acccagtaaa acatgcctga taagcagaac gtgatcacca atacaatcgt tgtcgcactg
    17701 catagccatg tgaataatcc agcaatcaat gctaatgcca aggcaatttc aaagaatacc
    17761 atgaattttt ggaagaacat tgctacatca ttgtttggca tgatcacttg catgatcttt
    17821 gtgaaccagt ctggtgactt ctcaatcaca gccattggtt cttcaccgta tgcataactc
    17881 aaaccaaaat gtgcagcttg agtcgctgct tctccggcac ttgcccctgc tccgctggca
    17941 gctgtggttg catcaccagc agcagaagca ccactggcaa catctgttac attgtattcc
    18001 catgtccaag ggaacacact gtttccagtg aaccatgatc cttcaccaaa ccaagtactt
    18061 ggtttgaaca cttcaccatt cccaatgatt tttttggaag cttcaacgaa ccaaactaaa
    18121 ccatagaaaa tacgaagtgg cacactccat aagacattgc cgtaacgaga agaatgtcct
    18181 cgagtcacgt tgcgatcatc tttgatatgg aaaaactcat gcattaaata ttggaacata
    18241 taataaccag aacgaatatc aaagaagtat ttcaaattca cgatatgttt cattacgata
    18301 gctaaaaatc cgcttaaatg gatcttatca aataggttag ctacccccca tttggcacca
    18361 atagaaacca taaatccttg atagtttcct ttaaatttat gcttttctcc gcctttgatc
    18421 gaagccacga tattcgctgc ggctgtgtgt cctgtttgtt cagcagcttg aacgatctgc
    18481 ggtgtcggtg ttcctgggaa ttcttcataa aagactaaat caccaatgat atagatgttt
    18541 ttatcttcgt agccttttgc ctgcatatat tcattggcga tcaaccgctg tccgcgtgct
    18601 gattctaatc caaagtcagc agcgtcagaa gttcctttga caccagctgt ccaaatcagt
    18661 gtgtgagtcg gaatgttgcg tccgtctttc aatttgatat gatcagcagc cacttctacg
    18721 attggtgcat tcaataataa ttccacgttg tgcttacgca aatagcgttc agcttttgca
    18781 gcatcatttc gatccaacat attcaagatc gtcggcattg cctctacgac catcagtgtg
    18841 atttcgtttg gatctaattt ataatctttg gctaggcgat ttttccagtc gatcagttcc
    18901 ccaatcattt caataccggt aaatccagaa ccacagacaa cgaaagtcag catggcttta
    18961 cgcagttcat catctggttc caatgcagct tttgctactg tcttctcaat atgttcccga
    19021 atccttacag aatcttcaaa tgaccaaagg gtgaatccgt gtgctttgac accaggtgta
    19081 ccaaagtcat tcggctctcc gcccattcca aggatcagct gatcaaaagg ataggaacct
    19141 aatttggtct tgacgatttt tcgttcttta tcgataccga ttacagtgtc tgtgacaagt
    19201 tgtacattct tttgacgagc aaacaaatgc tgcagatcgt attggattgc ttctggttct
    19261 acacgtccgc cagctacttc atgcaactcg gtcatcatag tatggtacga atgacgatcg
    19321 atcagagtaa ttgctacatc ttcttttttc agtttctttg ccaagtattt tgtagctgaa
    19381 acaccggcat atcctgctcc tacaacgaca atattttgct ttgccattga tcatccgctc
    19441 cttatagact atctatataa tgaatatatg ataattcttt tagtttgata cttattacac
    19501 aatctccata tattatatag atgttctgtt cttttgtcta actccttttg gatttttttc
    19561 aaattttaaa ataaagcgtt ttttcacgaa atttgctgaa agaaaacgtt tgaaatatta
    19621 catgggttat ttatcaaaaa tagttgactt ataggctaaa aagattagaa tgtataatga
    19681 atcacaaaga aaaattgtgg actagaaatg gaatggtggg gtattaaaat gaagaaaaaa
    19741 cgttttgtaa ctggatttgc cgtattagca ttttcagctc tagtgttagg cgcttgcggc
    19801 gcagatgaca acggaagctc aaactcttct tctgaatcat ctacagcaca atcttcgact
    19861 gcaaaaagca caacagaatc atctgcaaaa gttgtagctg gcggagattt gcaagacggc
    19921 acatataaat tagaagaaaa aaattattca aatggttacc gtgcagtctt tgaaatggtt
    19981 gtaaaagacg gcaaaatcac tgaatcaaaa tatgataacg tcaatgaaaa cggcgaatca
    20041 aaaacaaaag atgcagaata caacaaaaac atggaagcaa aatctggcac aaacccagaa
    20101 aaatacattc ctgagttgaa tgaccaattc ttaaaagcac aaagcgcaag cggtgtggaa
    20161 gtcgtaactg gagctactca ctcatctgaa agcttccaaa actacgcaca acaattgatc
    20221 caagctgctc aagctggtaa cacagataca atcgaaatcg acaatggtgc tgatttgaaa
    20281 gacggcactt acaagcttga agaaaagaac tactctaacg gctaccgtgt gcaattcgaa
    20341 atgacagttg ctggcggcaa agtaactgaa tcaaactttg attatatcga caaagacggt
    20401 aaatcaaaac aagatgatac agaatataat gaaaatatga aagcaaaaag cggtacagaa
    20461 ccaaaaactt acatccctac tctgaacgac gaattcgtca aagcaatggg cgaagaagat
    20521 ggctcacctg ctaatgtaga agttgttaca ggtgctacac acagctcaca ttcattcatc
    20581 atgtacgcac aacaattagt gaatgcagct gaaaaaggcg acactcaaac aatcgaagta
    20641 gataatatcg taacagaaaa ataatcacat aatcatacta ataaaagttg gagggtggga
    20701 cgatagccag attaggcagt cgtctcacct ttcaaaatga agagaggaaa aagatgcgga
    20761 aaaaactact attttcctta atcacactgg gcatctcttt tatggtgcta actggctgca
    20821 gcaactcagc aaataaaaca accactgaaa gttcagcagc taccaagata ttgaaagatc
    20881 catattctga tgaacagttc ttgttaggga cttacgttcg tatccgtgtg tatgatgaag
    20941 gcaaagaatc agcattgaaa cctgccttcg atcgagtaaa agaacttggt gataaaatca
    21001 cgatcaatca aaaaggttct gagatcgatg aagtgaatga gcaagctggg atcaaaccag
    21061 taaaagtttc tgatgacgtc tacacgttag tcaagcgggc ctacgaatat agccaagatt
    21121 ctcaaggcgg attcgatatg gcgattggtg caatcacaca attatggcga atcggttttg
    21181 atgacgccag aaaaccttcc caagaagaaa tcgatcaggc attgaaatta gtggattatc
    21241 ataagatcga actaaatgac aaagaaaaaa ctgtttatct aaaagaaaaa ggaatgatca
    21301 tcgatttagg tgcaatcgcc aaaggttaca ttacggacga agtagtgaaa gtcttgaaaa
    21361 aacaaggagt gaccactgcc atcgttgact tgggcggtaa tgtctatgtc ttaggccaca
    21421 gcccaagagg cgaaaaccaa gactggacag tcggcatcca agatccgaac atggctcgcg
    21481 gatctgtttt aggttctatt aaagaacgga acaaaacatt agttacctct ggaatttacg
    21541 agcgttattt agaagtagat ggcaaaaaat accatcattt gtttgatcca aaaacgggtt
    21601 atccgttcga caatgatatc gcaagtgtca cgatcataac agataaatca attgatggtg
    21661 acgggttgtc tacagcagtc ttttcaatgg gtgtgaaaaa aggactggaa tacgtcgaaa
    21721 gcgaattgaa taacgggacg gaagcgatct tcgtgacaaa agacgataaa gtatatgtga
    21781 ccgatccaat caaagacacc ttcaaattat cggaggactc ccattacacg atgggtgacc
    21841 gttctgaact aaaataagga gtacgaacca tgacactaaa aatcttttta gaagtagttg
    21901 aaatacgaac aaaggtggct agtgtatttc cttttgcggt aggggtatta ttctctatcg
    21961 ctttttttca tgagatacat tggctaaaca cttttttatt ttttattgga atgctagttt
    22021 ttgatttagc gactaccgct atcaataatt acatggatta tgaaaaggct cattctgaag
    22081 tatataagta tgaagaaaat gtcattggcc gcagaaatct atctcctgta ttgattcgaa
    22141 acatgatttt agctatgatt gcatttgttt ttgttatcgg cgttattcta acgatcaata
    22201 cgggttggct tttacttttg atgggattta tctgttgttt tattggagta ttctatacat
    22261 tcggaccgat tcctttatct cgaatgcctt taggggaaat tttcagtggg gtgactatgg
    22321 gccttggaat ttttgccatg gtcgtttatg tcaatacatt cggttcagat gtctatgatc
    22381 tgacagtcaa cttttcatct ggacaatttg ctttggtagg aaatctttgg gctatagcag
    22441 caatcgtatt agcttcctta cccttggtgt ttacgattgc caatatcatg ttggctaata
    22501 atttgcggga tcttgaccgt gatattgaaa atcatcgcta tacactagtt ttttatatcg
    22561 gacgtcctgc gggaacagtc ttattccagc tattgatgta tgcttgctat ttcgtgattt
    22621 taatggggtt gttgagcggt atctatgaat ggccgatctt ggtcaccttc ttgaccttgc
    22681 caattgtgaa gaagcatttg gatcaattca agcaaacgtt accccagcct atcagtttca
    22741 gctattccat caaaaatatg gtcttattca atgccagtta tgtattgggg ctattactaa
    22801 caatcattat gcaagccatt taacagcatg aagaggttgt gaaaaaactg ccctgcatca
    22861 agtaataaga acagccaaat aaaaatagct ccttatattt ttcgtttggt tgggcttatt
    22921 accgcagatg caagcttttg aacaccgttt atca
//
LOCUS       DAYYCW010000042        22926 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000042 DAYYCW010000000
VERSION     DAYYCW010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.042"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..371
                     /locus_tag="ACHAYA_001443"
     CDS             3..371
                     /locus_tag="ACHAYA_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM8605244.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="ACHAYA_001444"
     CDS             551..1492
                     /locus_tag="ACHAYA_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM8605245.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="ACHAYA_001445"
     CDS             1561..2292
                     /locus_tag="ACHAYA_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8605246.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="ACHAYA_001446"
     CDS             2436..4616
                     /locus_tag="ACHAYA_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM8605247.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="ACHAYA_001447"
     CDS             4636..5118
                     /locus_tag="ACHAYA_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM8605248.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="ACHAYA_001448"
     CDS             5134..5895
                     /locus_tag="ACHAYA_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8605249.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /gene="murC"
                     /locus_tag="ACHAYA_001449"
     CDS             6026..7360
                     /gene="murC"
                     /locus_tag="ACHAYA_001449"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM8605250.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="ACHAYA_001450"
     CDS             7667..8152
                     /locus_tag="ACHAYA_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM8605251.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="ACHAYA_001451"
     CDS             8145..8828
                     /locus_tag="ACHAYA_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM8605252.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="ACHAYA_001452"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="ACHAYA_001452"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM8605253.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="ACHAYA_001453"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="ACHAYA_001453"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM8605254.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /gene="coaE"
                     /locus_tag="ACHAYA_001454"
     CDS             12444..13073
                     /gene="coaE"
                     /locus_tag="ACHAYA_001454"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM8605255.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="ACHAYA_001455"
     CDS             13125..13457
                     /locus_tag="ACHAYA_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8605256.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="ACHAYA_001456"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="ACHAYA_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM8605257.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="ACHAYA_001457"
     CDS             14283..15656
                     /locus_tag="ACHAYA_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8605258.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="ACHAYA_001458"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="ACHAYA_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM8605259.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="ACHAYA_001459"
     CDS             16711..17151
                     /locus_tag="ACHAYA_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM8605260.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17295..17996
                     /locus_tag="ACHAYA_001460"
     CDS             17295..17996
                     /locus_tag="ACHAYA_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM8605261.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17993..19114
                     /locus_tag="ACHAYA_001461"
     CDS             17993..19114
                     /locus_tag="ACHAYA_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM8605262.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19129..20361
                     /gene="pepT"
                     /locus_tag="ACHAYA_001462"
     CDS             19129..20361
                     /gene="pepT"
                     /locus_tag="ACHAYA_001462"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM8605263.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20493..21401
                     /locus_tag="ACHAYA_001463"
     CDS             20493..21401
                     /locus_tag="ACHAYA_001463"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8605264.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21436..21708
                     /locus_tag="ACHAYA_001464"
     CDS             21436..21708
                     /locus_tag="ACHAYA_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM8605265.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21719..22765
                     /locus_tag="ACHAYA_001465"
     CDS             21719..22765
                     /locus_tag="ACHAYA_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM8605266.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7731 a         3990 c         5023 g         6182 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17281 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17341 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17401 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17461 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17521 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17581 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    17641 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    17701 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    17761 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    17821 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    17881 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    17941 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18001 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18061 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18121 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18181 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18241 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18301 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18361 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18421 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18481 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18541 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18601 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    18661 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    18721 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    18781 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    18841 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    18901 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    18961 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19021 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19081 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19141 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19201 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19261 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19321 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19381 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19441 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19501 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19561 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19621 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    19681 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    19741 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    19801 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    19861 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    19921 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    19981 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20041 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20101 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20161 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20221 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20281 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20341 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20401 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20461 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20521 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20581 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    20641 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    20701 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    20761 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    20821 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    20881 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    20941 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21001 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21061 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21121 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21181 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21241 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21301 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21361 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21421 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21481 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21541 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21601 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    21661 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    21721 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    21781 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    21841 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    21901 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    21961 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22021 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22081 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22141 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22201 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22261 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22321 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22381 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22441 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22501 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22561 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22621 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    22681 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    22741 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    22801 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    22861 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
    22921 tatatt
//
LOCUS       DAYYCW010000043        22830 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000043 DAYYCW010000000
VERSION     DAYYCW010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.043"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..210)
                     /locus_tag="ACHAYA_001466"
     CDS             complement(<1..210)
                     /locus_tag="ACHAYA_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605267.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIN"
     gene            complement(301..753)
                     /locus_tag="ACHAYA_001467"
     CDS             complement(301..753)
                     /locus_tag="ACHAYA_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8605268.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(766..1176)
                     /locus_tag="ACHAYA_001468"
     CDS             complement(766..1176)
                     /locus_tag="ACHAYA_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8605269.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1451..1663
                     /locus_tag="ACHAYA_001469"
     CDS             1451..1663
                     /locus_tag="ACHAYA_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFM8605270.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1666..1878
                     /locus_tag="ACHAYA_001470"
     CDS             1666..1878
                     /locus_tag="ACHAYA_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605271.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            2018..2173
                     /locus_tag="ACHAYA_001471"
     CDS             2018..2173
                     /locus_tag="ACHAYA_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605272.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2170..2394)
                     /locus_tag="ACHAYA_001472"
     CDS             complement(2170..2394)
                     /locus_tag="ACHAYA_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8605273.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2472..2660
                     /locus_tag="ACHAYA_001473"
     CDS             2472..2660
                     /locus_tag="ACHAYA_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605274.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2799..2927
                     /locus_tag="ACHAYA_001474"
     CDS             2799..2927
                     /locus_tag="ACHAYA_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605275.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            2986..3321
                     /locus_tag="ACHAYA_001475"
     CDS             2986..3321
                     /locus_tag="ACHAYA_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605276.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3554..4495
                     /locus_tag="ACHAYA_001476"
     CDS             3554..4495
                     /locus_tag="ACHAYA_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFM8605277.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4497..5387
                     /locus_tag="ACHAYA_001477"
     CDS             4497..5387
                     /locus_tag="ACHAYA_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM8605278.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5430..6230
                     /locus_tag="ACHAYA_001478"
     CDS             5430..6230
                     /locus_tag="ACHAYA_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM8605279.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6245..7096
                     /locus_tag="ACHAYA_001479"
     CDS             6245..7096
                     /locus_tag="ACHAYA_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8605280.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7297..7758
                     /locus_tag="ACHAYA_001480"
     CDS             7297..7758
                     /locus_tag="ACHAYA_001480"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8605281.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7784..8236
                     /locus_tag="ACHAYA_001481"
     CDS             7784..8236
                     /locus_tag="ACHAYA_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605282.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8342..8536
                     /locus_tag="ACHAYA_001482"
     CDS             8342..8536
                     /locus_tag="ACHAYA_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605283.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8567..8953
                     /locus_tag="ACHAYA_001483"
     CDS             8567..8953
                     /locus_tag="ACHAYA_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605284.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            8950..9153
                     /locus_tag="ACHAYA_001484"
     CDS             8950..9153
                     /locus_tag="ACHAYA_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM8605285.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            9150..9446
                     /locus_tag="ACHAYA_001485"
     CDS             9150..9446
                     /locus_tag="ACHAYA_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605286.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            9522..9938
                     /locus_tag="ACHAYA_001486"
     CDS             9522..9938
                     /locus_tag="ACHAYA_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8605287.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            10199..10336
                     /locus_tag="ACHAYA_001487"
     CDS             10199..10336
                     /locus_tag="ACHAYA_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605288.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            10746..10976
                     /locus_tag="ACHAYA_001488"
     CDS             10746..10976
                     /locus_tag="ACHAYA_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605289.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            11135..11968
                     /gene="terS"
                     /locus_tag="ACHAYA_001489"
     CDS             11135..11968
                     /gene="terS"
                     /locus_tag="ACHAYA_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFM8605290.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11961..13376
                     /locus_tag="ACHAYA_001490"
     CDS             11961..13376
                     /locus_tag="ACHAYA_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFM8605291.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13388..14917
                     /locus_tag="ACHAYA_001491"
     CDS             13388..14917
                     /locus_tag="ACHAYA_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8605292.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14835..15887
                     /locus_tag="ACHAYA_001492"
     CDS             14835..15887
                     /locus_tag="ACHAYA_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFM8605293.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15889..16206
                     /locus_tag="ACHAYA_001493"
     CDS             15889..16206
                     /locus_tag="ACHAYA_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605294.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16333..16956
                     /locus_tag="ACHAYA_001494"
     CDS             16333..16956
                     /locus_tag="ACHAYA_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFM8605295.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16971..17861
                     /locus_tag="ACHAYA_001495"
     CDS             16971..17861
                     /locus_tag="ACHAYA_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFM8605296.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17884..18099
                     /locus_tag="ACHAYA_001496"
     CDS             17884..18099
                     /locus_tag="ACHAYA_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605297.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18111..18452
                     /locus_tag="ACHAYA_001497"
     CDS             18111..18452
                     /locus_tag="ACHAYA_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605298.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18452..18823
                     /locus_tag="ACHAYA_001498"
     CDS             18452..18823
                     /locus_tag="ACHAYA_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605299.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18816..19211
                     /locus_tag="ACHAYA_001499"
     CDS             18816..19211
                     /locus_tag="ACHAYA_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFM8605300.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19213..19590
                     /locus_tag="ACHAYA_001500"
     CDS             19213..19590
                     /locus_tag="ACHAYA_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFM8605301.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19591..20199
                     /locus_tag="ACHAYA_001501"
     CDS             19591..20199
                     /locus_tag="ACHAYA_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8605302.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20199..20540
                     /locus_tag="ACHAYA_001502"
     CDS             20199..20540
                     /locus_tag="ACHAYA_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605303.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20782..>22830
                     /locus_tag="ACHAYA_001503"
     CDS             20782..>22830
                     /locus_tag="ACHAYA_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFM8605304.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     L"
BASE COUNT         8246 a         3315 c         4690 g         6579 t
ORIGIN      
        1 attaatatca ttttttaaca tagtaatttg tttatttaaa tctgatagtg tttcttcctt
       61 acttaatata aatctttctc tatcatgtaa tttatttagt ttttcatcat ttttttgttc
      121 taattcataa gcttcttttt ttattgtgtt agctttcgac agtttagtga ttcctataat
      181 taataaaata agtccaatca ataataacat tcaaaactcc tcatttcttt gatataatta
      241 ttgtagagtt tactcagaaa tgaggaaaga gtccatgtga aagcatgggc ttttttattt
      301 ttaatgttgt aatactaaat gttcaagatt atgaggaagc cctaaatatt caataatttg
      361 gaactgactc atatcagata aatcttgttc atctattgtt ctagttaaca tttcaattgc
      421 aaacatatta gcttcagctt ctataccata aatcatacaa ctaaggttgt ttgctctaaa
      481 agctgctgtg caaatacctt tatggagtct aacgtgggct atttcatgcc ataatacaaa
      541 tcttttcata gaaggtgata ggttttgatt aattaatatc acataacatc ttttattgtg
      601 tatcgtttgt cctaatgttt tattccctaa atcagtttca attaaatcta tatcagaata
      661 tttgattatt ttatatggat taatcgcatc agatccaaac tctacaaata atttttctat
      721 ttcttctaat atccagtatt tgtttaaatg cataccaaag cccacctatt gacgatattt
      781 tttaggagtg aattttttct ttgctttaac tttggttaag gcaagtgcaa gttgaatggc
      841 atcgtataat ttttccttat cttcatcgtc taaaggttca ccataataat ttgtttcagc
      901 atctgaattc aagccttcaa gtaatttttc agcttgtaaa gcaatatctt gtttatcttt
      961 ttctgttaaa tcataataat ggcgtttatc agtacgtcct aaaagataat cagttgatac
     1021 attaaagtaa tctgcaactt tttgtatttt atctccagat ggagtacttt tattccatct
     1081 agacatgctt ccatttccga aatttaactt tctttcaagt tctgccaacg tcattgaacg
     1141 ttcttttaat aagattttta tacggtctaa tagcatagga tatgcattcc tttcaaaata
     1201 ccgacaaaaa aagtattttt ctgcaaatta tctatttaca aatagagaat ttgcagttat
     1261 actagttttg taagcaagat atttacggca tacaaagact aaaagcaata aactaatagt
     1321 ctccccagac atcagtaagc tcgctttttg tgcgctgtat tttgtatgct tacataatag
     1381 catatttgca gtttttgtca aacttattag acaaaaatat tagaaaatat tcgataaggg
     1441 ggtatgaagc atgactttaa aacagcaagt attaattgct ttagtaaaga aaggttggag
     1501 tcaaagagag ttagctcgac gaatgggtat atcaattact tatttacgag atatcttcat
     1561 cgaaaaaaga aaacctaagg aacgtttaaa acaaatcgaa gaacttttgg atatcaagtt
     1621 agaagtagat tctaatgatc agccatcaga acaggaggcg taaaaatggt acaacgtttg
     1681 agcaaagcgg agtttaaaga gaaatataag tattctgaaa gtacttatca aagaagaatg
     1741 aaggaattta aaaaatcacg ttttagtgag gggtataacg cagttacttc caaagaaata
     1801 acaattgatg tcgatttata tgaacagttt agaacatggc gttctaaaaa tcgatttaga
     1861 gttagagctg tttgttaata gctaaaaaat ctaaattcac cacttctgaa aagaggagtt
     1921 gctgctctcc tttcaagttt gccaaagtcc acgttttttc attttggcaa ctcctcttct
     1981 tggaggtggt gacggcgaga gacgaaggag ggatgacatg aaggaagaat caaaaatagg
     2041 gattttgaat gagaacaatt acacaagatt aattattgaa acagaagaag ggaaaaaagt
     2101 agcagaagtt acattgaccg atgcaactcc tgctgacggt tatcgtattc gattaactcc
     2161 taaatatgat taaccttttg gtgggtgtgg atcgtttccg tgactatcac ggctttgaat
     2221 ttttccatct ctgccatgaa taaatagctc agatccttga tttttagaaa tctctctagc
     2281 gatttttact gcttctgatt ttgtattagt atgtgcagtc gctttagaat ttccagcacc
     2341 tttgacattc caaccaccat tggaattagg tacaacatgt tgattttttc ccatagtttt
     2401 cacctccatt tctatagaaa tattttatca aatagaaatg gagaaattca ataaacgaaa
     2461 ggagaagtga tatggcatat accttgcaac aagaacatca aattctcggt ttgattaaac
     2521 aacgcaggaa acaattacaa gatgaccgtg cagcgcttag aaaagccgat gagctatcag
     2581 ataggcaagc tgaactaatt gcttctgaac ttgaggattt gagaatgcta gaaataaaaa
     2641 atagggagat tagattatga agaagacaga cacacttttt ataggattca ttttggggtt
     2701 attagtgatt gtagcgcacc aaagtattat cgggggaagc ttgttcgcag cattgatggt
     2761 tttaatcaat ctgcttgatt caaaagaaag gagcaactat ggcacgagaa gaagcgctaa
     2821 aaatcggtaa agtgattgct gataatcggt ggggaaatag ccgccctatt attttaagca
     2881 agcaacatat cgaaaagcaa aaagcatggc aacaaataaa aaagtgactc tgccagccag
     2941 caaagagtca catacaaatt tataaaacta ggagaattat atcacatgaa taagaaaatt
     3001 gaaaatctta tcgaagaatt gaaacgtgaa tgccaaaagc aaggtgtttc tattatttgt
     3061 acagcccaaa aagaagggga acttaaaagt ctcgtttatg gtgagacaac agaaatttta
     3121 ctttgtcttg ctatgcaaga agaacattta gatgaaaatc tcccattgtc agcacatatt
     3181 atgcgtagaa ttgctgtgga tgcttacgaa caagcgaaaa atgaagaaga aaatcaacct
     3241 tcaaatcata cttttgttat taataacaag gaagatttag ctgatgtgat gactcgtatt
     3301 ctcaaggggg aatttcaatg atgaatgaaa aaacaattga tctactcatt attttatttt
     3361 tcacgttagt gattgtagcg aaaagttatg taatctttgg cttgttattc gcaattacca
     3421 tgcttgtgct ttgcgtgtta gcaagcagaa aagaggttaa acatgaacga gttagcaaat
     3481 cttgacaact atttaactga tcctgattat gctaaaccac catatgaagc accaattgat
     3541 gaggaggatg aagatgagta aatctacctt agaaatgagc catcaagaat ggcttgaaga
     3601 ccgtaagaaa ggcatcggag gttcggatgt tggaacaatt ttaggattga ataaatggaa
     3661 atctccttat caactatggc tagaaaaaac aggacaagta gtacttgaag aatcaggaag
     3721 tgagcccgct tattggggta atgttttaga agaagtggtt gctaaagagt ttcaggaacg
     3781 tacaggcaaa aaagtacgca gaagaaacca agtatttgaa catccattac atccattttt
     3841 aagagcaaat attgatcggg aagtagtggg agaaaatgcc attctggaat gcaaaacagc
     3901 taatcaattt ctcggtaaag aatgggaagg cgaagaggta ccgcttagct atctctgtca
     3961 agttcaacat tacatgaacg ttctaaacaa agactattgt tacatcgctg tcttaatcgg
     4021 tggtcaaaaa tttatctgga agcggattga acgagatcaa gagctgatcg atacaatcac
     4081 tgaacaatta gtagagtttt gggaaacaaa cgttcttgga ggtatcgagc caattattga
     4141 tggtagtcaa gcgacagctg atttcttgaa agagaagtat gcagatgttg aagacgttca
     4201 aacgacgtta ccaattcatt tcgatgaact agttgaacag aaaaacgaac tcaaacggac
     4261 taagaaagaa attgaatcag ctatccgtca agtagacaac gagattatca gcgagctagg
     4321 taaacgtgaa gccagtatcg gtatcactca aaaaaacatc atcagttgga aatttgtccg
     4381 tacgagacgt atgaactcga agaaactagc agagaaatat ccagatgtcg caaatgatga
     4441 agagatttat aacgttactg aatcaagaag gctaaccgaa agggagatca aataatatgg
     4501 caacaaatga atcgttaaaa aatcaattgg cagcaaagcc acagaaacaa gttgcaccag
     4561 gtcaattagg acttaaagct ttgatgaata caccaacaat gagaaagaag ttcgaagagg
     4621 tgcttcatga caatgctaat gcttttatgt cgaatgttat gactcttgta tctaatgaca
     4681 gttatcttgc agatagtgaa ccgatgtcta tcatgagtgg tgcgttaact gctgcaacat
     4741 taaatcttgg gctagataag aatttaggtt atgcatattt agttccattc aatagtaaaa
     4801 acaagcaaac aggaaaatgg gaaaagaaag ctcaattcat gcttggctat aaaggatata
     4861 tccaattagc ccaacgatca ggtaaataca aagcattaaa tgtgatcgaa gtttacgaag
     4921 gagaactaaa aagctggaac cgactgacag aagagtttga gtttgatcca aatggtagaa
     4981 cgtctgatga agtcattgga tatgttggct atttcgagtt actgaatgga ttcaaaaaaa
     5041 ctgtctattg gaccaaacaa gaaatcgaag ctcatcggat tgctaacaat aaagatcgcg
     5101 ataagacaaa gttaagtggt gtgtgggcat ctgattacaa tgcaatggca cgaaaaactg
     5161 ttttgagaaa tcttctttct aaatggggaa tcttgtccat cgaaatgcaa gaagccacca
     5221 catcggatga gagagtccaa agggttcaag aagacggcag cattattgct gaaacagaag
     5281 ttgaagaaga tattcctgaa agaaaagaag cagaggttat atctgaagaa aatgaagatg
     5341 tacaaactgg attatttgat gcatctaatc cgccgttaaa caaataatga gggagctttc
     5401 tcccttgctt ttctagagga gaaatacgaa tgaacacagg atatgtcaaa ttacatcgaa
     5461 aagtgatgaa ttcattcgtt tggaccaatc cttatatgta taaattatgg aacttgtgtt
     5521 tgatgaaagc gagtcatgag aatcgcaaga tactttttaa tggaaaagag atacaagtga
     5581 acagcggaga attcgtcaca gggcgcgatg ctatcacatc tgagatgaac gaaggcgtga
     5641 aacgtgaaca tcaagtgaac agcggttctg tatggagatg gctaaaacaa tttgaaaaaa
     5701 acggaatgtt gaacatcaaa tcaactacga aatacagcgt tatatcaata aaaaactggt
     5761 ctttgtacca aagcagtgaa caacaaatga acatcaaacg cacaacaagt gaacaacaag
     5821 tgcacacaaa caagaatgaa aagaattata agaatgaaaa gaataataaa acatcatcgt
     5881 cgcaaccacg caaaaagcgt gtttacgaca ccgactcagt ttactacatt ctcgcggagg
     5941 agttattcaa acagatttgt cagaatcagg aaatcaaaaa gccgaatctg caaagttggg
     6001 cagataacat tcggaaaatg atcgagatcg acaagcggac cgaaaaccag gtacggggaa
     6061 tgattgaatg gagtcagcac aatgtgtttt gggcatcgaa tattttatct gctaaaaaat
     6121 tgcgagaaaa atacgacaca atggcagcgc aagccaatcg tgattataaa acaaaacaaa
     6181 ctaaaacgct tgaatacgag aaatttagca cagatgagtt gcctatttga gaggaggcgt
     6241 aggcatggaa actgttgggg aaatcatgga aaagctgata cagaaagtgc ttgtccaacg
     6301 tggcgaatgt cctgaatgcg gacagccttt gtatggatgg cgtacgaaga accctgatgg
     6361 ctcagaacgt tgtaagccaa cctgtatgca atgtgggtat aaagcattgc gagttcagga
     6421 agacttacaa acagaacgaa tttacaacga gagcctgaaa gcaagagcaa tcaatttttt
     6481 caaaggtggt tctgttgtgc ctaatcaagc gttgtttgat tgcacattgc agaattatca
     6541 aattgtcgat caagaaacaa gacaagcggt tgaagtaacc aaacgctttg ttaattcagt
     6601 cttgttagga aatccaagtc accttgtttt aactggaaaa caaggaacag gtaaaagtca
     6661 cctagcgatg gcagcggctt gggaagtctt gaagcgatca aactacgata aaaaaatctt
     6721 atttatcggg ttacaggaaa tgctggatca aatcaaattt tcgtacaaca atcctgaact
     6781 cagaaaaacg attgagggat cgttgattgc agatatcaaa acagcggatt tagtcatcat
     6841 tgatgatatc ggttcagaac taggaaaaga tgcatcagat agtcgagcgt ttggcataaa
     6901 cacgctaaat tcgttcttgg atgcacgaca gaacctagca acgattatca caacgaactt
     6961 gcttggtgaa gaactgaaaa aagcttacgg tacgagaaca atatcaagaa tgtttgtcaa
     7021 ctctgatgga tttacgatgg tattttctca aacagcagac aagcgcataa aaccagtcaa
     7081 aggagcgatc gcatgaattt gactaaacaa gaaaaaagaa tctgtcgtca gataatgttg
     7141 caaattatag gtgatcgtga catggacgaa ttatctcaac aagagagtcg aatggttcgg
     7201 aagataagta agctaattat ttatcgggat acagaaagat ttctcactgt tttaggtaac
     7261 gtctacgaga atccagagtt attggaggta gagtaattgc caaaaatatt agatgcatgt
     7321 tgcggtagta gattgttctg gtttgataag aacaattctg acgtaacatt tatggattgt
     7381 cgtcagcagt atgaagaatt atcaacaggt catgtgatca atgttgatcc agatgtggtc
     7441 gcagactttc gcgatatgcc ttttgaagat gatgtgttcg atatggttgt atttgacccg
     7501 ccacacttaa gacatgtcgg cgaaaactca tggcttgcca aaaaatatgg aaaattagac
     7561 gaactttggc cagaagacat caggcaaggt tttgcagagt gtatgcgagt tttacgacct
     7621 tcaggaacat taatttttaa gtggaacgag gaacagatac cactttctga tgtgttagag
     7681 gctattggag aacagccatt gtttggtaat aaacgaagca aaacacattg gcttgtgttt
     7741 atgaaaaaat tcagctaacg gaggaaacaa aggaggaaaa gtaatgaaga ttgtattaaa
     7801 taaatgtttt ggtggctttg ggttgagcca tgaagcaaaa atggaaattt ttaaaagaaa
     7861 aaatattgaa gtattcccat acatcaataa ctttagctat gactcagatg atgaatacac
     7921 gagatacaca ggccaaaagc taggttcgat ggattttatc tactatttca aaaaagaccc
     7981 gaaaatcgac aaagttacag gaatttatag cgaaattgac cgactgtacg gaattgctga
     8041 tgattccagt ttttcaagtg attcaaacag aggtgataag gacctcgttg ctgttgtcga
     8101 aaaactagga agtgaggcca gtggaccata tgcaagtctt aaagtcgttg atatacccga
     8161 tggggctgag tgggaaataa gtgattatga cggcgttgaa actgcacatt atggttttca
     8221 aacaggtagt atttagtcca ctaatgacaa ctaaagagga ggcagaagga tgagtgaaga
     8281 tgaatacttt gaacagtagt ctattaacga cagagatttt taactggaaa ggggtgtagt
     8341 catggacgca tcggaagtag taaataaaat taatagtctc aaagaggttt tcggagacgt
     8401 cgaagtagtg ataaacgcac aaggctatga caacggattt tttaagcaga ttaccgacat
     8461 tagcattcag caaggaatgg aagacgaaga tggaaacttt atcgatgaag tagccatttt
     8521 agtgacttgc gagtaagtaa ttgacagcta aagaggaggc agaaggatga aaaaattgca
     8581 ccaactcata tctgagaaag aaagcgaact ccaaaattta gaagatagtt tgggattggg
     8641 gtttccaatt gttgagcaag ctaaaatgac tcaaatcagt catctacgac ttgaattgga
     8701 agatttaaga caaatagaaa aatcaattca actcaacgac aaccagaaaa tcgtgttgga
     8761 gtggttgaaa agcgagacaa ttttaactag agaagcacca atattatctg ttaatgcttt
     8821 ttctgataaa aatttactag gaaaattacc tgataaagta cgcaaagctt ataaactatt
     8881 ggcttgtaaa caagaatatg aagtcctatc agcattcgct caatggggat tagaacagga
     8941 ggaagcggaa tgaacaacag acatcgcaga ataacaaaac taagaaaaca ggaactgaat
     9001 gtactaaaga caaagtttga aaaagaatat ggaatttcag cagaagaaac atataaagtg
     9061 gcaagtcagt ttgttgctga tgcgagtaat gctattcgta agtttgggat ttcgatatta
     9121 aatgatgatc gtaaatggga ggaaatgaga tgaaaataaa agacggattt tacgctagta
     9181 gtcatggtat cggcggttta atgctagaca tgccgataaa gaaccctaaa acacgtcaga
     9241 aatcaaaatt caaaatcggt gacatggtcc gatgtaaagc agaagagttc atctatccgt
     9301 ttcgtggata tgtagagcat gtctataatc attcagcaat cattcacatt gaaaacacga
     9361 tggaatgcga taagtggtta gcgaaaagca aagataattt agcagtggct cgtttggtgg
     9421 atatggaact aatcaatgac aaataaaaaa gccggatcgc tccgactgat tcaataaatc
     9481 caacacattt attatatcac ataaaggagc ggtttgactt gatgcaattg ttacgagagg
     9541 tagatttcaa acagacaaga tgtaatgcga gagatgtgct gaagaacttt cggcgtttgg
     9601 agcggatggc aggtcgctct ttgatagata ttaagtcgcc gattattacg gatatgccga
     9661 aggcaccgaa gcacggcaat aaggcagaag acgcgatcat tcagatgatg gatatagaag
     9721 cggagagaga cgcaatacta gcggctttga tggctcttag tctgattagc cgtcagatac
     9781 tctactacag cttctgtgac gtaaacaagc actctaatta tgaaataggg caattgatac
     9841 gaggatacgg agagaagaat gtagagaagt tgaaatccat cgcattaatc gaatttgcag
     9901 aagcatacaa aaaaggcgtg ttagttcagt atcgttgatt ttgtagggtt tttgtaggga
     9961 tagtgtaggg tttttgagcg gtttaacgtg atattatggt agtgtcgaaa gattagtgat
    10021 aggtctgaga caaaataaaa tgtaagggag gaaatctccc tcatcgtttt aacttaagct
    10081 tcgatagaca gcaacggaaa tattaagaat aaggatgtga atttcaactc cttctaaatt
    10141 gttcttatta tctatcatcc gttgttgtct attaatttat gcatcggagg aaaaacgaat
    10201 ggataaagaa atcaaagtaa ctgtcaaatt agatttgact gaactaaaag aactgctcaa
    10261 caaggctagt gaccaagtcg aacagttaca agaaacttta gatgaaattg ctaattttaa
    10321 aatccaagtt tcttagcagt atatttttta gcagctgcac tcataaaatc ataccaagta
    10381 tcagaaaaat ataacttaac taaataatta aaaaaggtgg gtaaatagaa atgacggcgt
    10441 taataatttc aatttttgcg ctctgtctta atgtctatat gattggattt aaaaatggac
    10501 aaaataaaaa atagtagcag ccgagaataa ttttatagtg tcactgtggc ggaaatagaa
    10561 gacgcaaagg tggaggtagg aagcgtatgg cgcactatcg ggaccttgta caaaactaga
    10621 aaggactagt acgtgtgtgg tgcgagccct agggaggaac aggataaccg cctgtgtgta
    10681 ggttgctatt acatattaga tcactctttg agtggtcttt ttatttacat aaaggaggct
    10741 acataatgag aaactactgg tatatatcgc taactaatga atatcctcga accattgatg
    10801 attgttcagt gcgtgttgtg cgttctgtcc aaatcaaaaa gaagtactcc attgttgaaa
    10861 tgaccagaga agctacgcca aaagagattg ataaatgcaa acttctttat tgcggtcatg
    10921 gtttctatga tgaaccaaac attcaaaata atattaacaa gaatttgagg gattagaatg
    10981 caagaaatgg cttaaaaatc tccaatcagg acaaatcaag ttagacaaag tttccgattt
    11041 aaagatattg attgaagctg atttaatgct aaaagatatt gaaaattaga aaacaaaact
    11101 caacctaaga agattgcgag gtggtgtgta ttgaatggca agacaacgtg atccaagacg
    11161 tgatgaagcc aaaagaattt ggttagaatc caacggagaa aagcagttaa aggaaattgc
    11221 atctgaatta aatgtttcag attctcaggt tagaaaatgg aaatcgcaag acaaatggag
    11281 cgctgaattg aaaagtaacg ttaccaatgg caaaagtaac gttactaatc aaggtggcgc
    11341 tcctattggt aatcaaaatg ctaaaggtaa caaaggaaac agcagagcat caccgccaaa
    11401 gagaaataag aatgctctta aaactggtga gtatgaaaca atattttttg acacgttaac
    11461 cgataatgag aaggacatct attctagttt gaatgatgat ccttcttttg ctttgtccga
    11521 agaaatacga cttcttaaaa taagacaact tcgaatgatg aaaagaatcc aacaagctga
    11581 agctggacta aatgatgaag aagtcgaacg attgcagcag ctaagaaaga ttaaaactcc
    11641 gattgaaaaa aatggtaaaa agctagaaat caagcgtgag gttatgcaag atgtgcagat
    11701 tagcagaaaa aaacatcgca aaattgatga tattctttca attgaagatt cattgactcg
    11761 gattagcaac cagttagcta aagccatcaa gcaaatgaat gaactttata tgaatgaata
    11821 cagaactgat ttaattaaag ctcagactga taagatccaa gctgagacaa atgaaattgg
    11881 cggaaataat tcaggtgaag aaatagaaga atggaaacag gcagttttaa atgccgcaaa
    11941 caaacgggcg gtgaaagaaa atgaataatg aatttattcc ttttgctgat attggttctg
    12001 ccattgatta ttactatgat aaaccagtag ctttttgcca agatattttg catttgaatc
    12061 ctgatgaatg gcaagaaaat gttttaaatg atttagctga attttcaaag gtttctgttc
    12121 gttctggtca aggagttgga aaaacagcat tagaagcagg agcaatactt tggttcttaa
    12181 cgtgtcgacc ctacgctaaa gtaatagcaa cagctccgac aatgaagcaa ctttacgatg
    12241 tactttgggc agaggtagct aaatggttaa atgatagctt gatcaaaaac ttactgaagt
    12301 ggacaaagac caaaatttat atggttggtg attcagagcg ttggtttgct acggctagaa
    12361 cagcgactaa accagaaaat atgcaaggtt ttcacgagga ccatatgttg attgtggtag
    12421 atgaagcttc tggtgtgtct gatccaatta tggaagctat tcttggtacg ctatcaggtt
    12481 ttgataataa gctgttgatg tgtggaaacc ctaataatat tgaaggtgtc ttttacgatt
    12541 cccacaattc agaccgtgat aaatacagag ttcataaagt atcaagctat gatagtaaac
    12601 gtacaaacaa agacaatata gaaatgattc ttaaaaaata tggaaaagaa agtgatgttg
    12661 ctcgtgtccg tatttttgga gaatttccca aaggtgcgtt ggattcattt atcagtcttg
    12721 aaacggttga attggctaca gaaaaacaaa ttagtgattc tttagtcaat aaaacaacgg
    12781 ttgctcatat tggtgttgac gtagctcgat atggtgatga ttctacgatt ctctttccta
    12841 gaattgctac cagggcattg gagtatgaga agtattcaaa acgtagcact atggaaacaa
    12901 caggatatgt catcaacatg gccaagaatc taatgagtca atatccgagt attgataaag
    12961 tgatgattaa agtcgatgac actggtgtcg gaggtggtgt aaccgaccgc ctagaagaac
    13021 ttatagaaga caagcattat ccttttgagg tgtttggagt gaataacggt tcaacatcag
    13081 aagacgattt ttacgataat ttaggtactc aactatgggg aaacatcaag gaaatgttag
    13141 aagaaaatat gacagcaaat cttaacggag aacagcctgt tattgaattg ccttctgata
    13201 gttcgttaat caaagaatta agtactcgca aattcaaaat gacaagtaga agtcgtatac
    13261 gtttagaaag taaagatgat atgaaaaagc gaaatattgg tagtcccgat attgctgacg
    13321 cactggcttt agcgttttat gagccaccaa gtcactatca atttattcaa ttttaggagg
    13381 tgagtttttg attagtacag tattatcttt agagagatat aagaagctac gtgttaaata
    13441 tgcaacacaa attgaagatg gaatgtttga tccgaatggt tttatagaat atatgaaacc
    13501 attttttgct gatcgcgaaa gaaaatatct agcttatact agcgaaaaga atgaaataga
    13561 caatagacca aagcctaaca ctgatattgt aaaagttaat aataaacttc atgctggtat
    13621 gtattcaatt gtcgtagacc aagcagtcaa tcattttact ggtataccta tcaaatggga
    13681 ttacgatgta tcggaacaaa aaagaacact cattcaaaga ttaaaagata aattcttaaa
    13741 aaatgatata gaacttccaa cagttccaga agcttttaac aaattaacaa gcaatcttga
    13801 ttccatgaga ttcgcaatgc ttgattctga aactgcaact tttcaaggag cttgtggagt
    13861 agctttccga ttgttagagc ctgtggaaga agatgatggt tggaagttaa gagcaagtaa
    13921 tattgaacct tggagagcag aaagatatgg aaatgccgga atctatatca aagaaaagta
    13981 tgactcttac cagaaaaaat tttttcaaga aatgaaagtt atgacaagaa ataaaattct
    14041 gacatatgct tgctatggtg atttgaattt tgttacaagc ggaacgttca aaaaaattga
    14101 tgagaccgat aaccctttgg ggacgattac cttggcagaa tttaaaaata atacgaatcg
    14161 ttattgtgat tttgaagtag ctgaggaaat tggagatgca attgatcgtg ctttgtctga
    14221 tcaacaaaac gaaatcgaac aatttaagct tgcgtacatg cttgttactg gcacaacaat
    14281 gagcaaaggc actgctaaag aaatgatgaa tcagctaggg attatcaact taaaagaccc
    14341 tactgcaaaa gctgaatatg tcacaaaaaa attagcaaaa gatttcaatg aatatcatat
    14401 ggatctattg aaaaaacagt tttacaccat ttgtaaagca atcgatttta acgatgaggt
    14461 gtttaaatca aacagttctg gagaagctcg caagtggcaa atcattagcc tagaagcgaa
    14521 aacaaatact aaagagcaat attttagaga aggattgaaa gaatgcgcag aaacaattgc
    14581 tgcttttctt aagtttcatg acaaagtaga aattgagcct gaaaaaatta tttttacttt
    14641 ctctagatct ttgccaactg atttaagtta tttagctgaa gctttaccta aacttgcacc
    14701 atacgtatcc aaacgaacta ttcagagcca aattccattt gtaacagatg ttgattatga
    14761 aaatgaaatg atggaattgg aaagtggaag tgcttatcca gatagtgaat acaattttgg
    14821 cggaggtggc aatagtgacg atagaaacga agtattggac caaacgtcga gaactggagg
    14881 atcaagcaag gctcaaacaa gaaaatcaga cacttaaaaa attaactagt gtatttcctg
    14941 aagcactgaa agagatacaa gcaaaactat tatcacaagc tgacttacac aatatcactt
    15001 atccagaaat gatggagttt tacagtacaa gtaatcagaa aaaatatcgt gaatatgtgg
    15061 aaaaaaatta taagtcatta aaaatgtatg atgcaaaata caaagagttt atcgatgaat
    15121 tttttccacc atttgactat gcaaaagtca atcgcttatt acaaatacga tcagatgtat
    15181 ttaaaattct tgcagaatat gcgatggatg cagatgtgaa tcaatatttt tctgatcgct
    15241 tagaggaaat tcttcaaaga acatattctt ctaatgctaa tgtttttgtt caacttttaa
    15301 acgttgatat accaaattat ttaccagaaa atgaaattaa acattatttg aactatccat
    15361 ggtcaggaaa gacattttca agaagacttt ggggaaatat ttcatcgctt gaacagaaac
    15421 tctctaatgc tattgtaaaa agtgttgcta gtggagaagg cgttatacac gcattaaaca
    15481 ccatgagatt agactcagaa atttgtgaca tgtttaagtt agaagaatca aagtataaca
    15541 aagcgataga aaatctcgtt cgaacggagt atgcaaaatt tgcacaagat ggtattgaaa
    15601 aatcatattt agaaacaggt attgaggaat acaacgtatt gactgcaaaa gatgagagag
    15661 tttgccggat ttgtggagga aaggcaagta agaatcccta taaactgaaa gatgctgtca
    15721 taggtgaaaa tcgagcacct ttccatagtc gttgtagatg tacggatgtt cctaatttac
    15781 caaaattagg aaaggatatt gatgaagaat atgaccgttt atttggcgat ttattagatg
    15841 agtttgcaca tgattctttt ggaattaatt tgaaacggag gaagtagaat gaaagatttt
    15901 tttgaagcag tactaacaat taatgtaaat gctgatattg cagaagccta caaaacagct
    15961 attgagtctg agaaccatcc taatggcttg agagaccatt ggaatggcaa ttatgcctac
    16021 gtggttattg gcgatcaaac tgttaattat caagataata ctccagttga taagaatacc
    16081 gttaatttaa cgattcaatt attatctcat tcattaccaa atttaaaaga aacagttgat
    16141 tggtatgaaa agatgggatg tattgttgtt agaactgact acaaagaagg aaagtctagt
    16201 aattaggctt ttttattttg tccgaaatga cgttaaacta gcgcaatact gggcttggtt
    16261 gaatggtggg gcgcaactat taaaactcaa agcaatgcgg ggcgtgaaaa cgaatcgtgg
    16321 ggcgaaagga gaatgagcat gaaacacaaa tcattaatgc caatgaattt gcaatacttt
    16381 gccgaaggtg acgatcaaaa gttttctttt gatgacttca aatcttttgt ggaatccaat
    16441 gaagaagctc aaaaatttat tcagtcacaa tctcaaagtg ttgccgacaa acaattggaa
    16501 gcttggaaac aaaataactt agataaaatt aaacaggata ccatcaagga atatgaggaa
    16561 tctaagaaaa acaagtcacc tgagcaaatt caactggaaa aactacaagc tgaatttgaa
    16621 gcagaaaaag cgttgcgtgt gacaagtgat aataaagcat ttgttgcaga acaaattgct
    16681 ggattagaac tagatggaga gttaaaagag tctatttctc aatttatgct aaataatctt
    16741 gttagttcgg atacagattt cactaagaat gctgttgaag gttttacaag tgttttgaat
    16801 gcaattaatg agaaacatgc agatgcatta aaagaactac aaatgaagtc tgcatttggt
    16861 ggaactcaac aatcgaataa ccaggttcag cagaacaatg aaacattaac aaatccagaa
    16921 gaacaattag gacaaattct tcaacaattt aactaggaga gtgaaaaatt atgaaaaaaa
    16981 catctttaaa taatttagag tatttggata tttcaccagc gattaatgct atgcaagtac
    17041 caaatacacc ttttttaagc tatttacttg gtgctggaaa aacagagcca gcaaactcga
    17101 cagaaattaa atggcgtgaa tatgatatca acaacgatga ttcttctgaa aaacttgagg
    17161 gcggagaata tccagatgct gaatcaggtc gaacttggtt taacaactat actgaaattt
    17221 ttagaaaatc aacctctgta tctggtacat tagatgctat taatgtgaat ggagtcggaa
    17281 atgaattaac taatcaagta gcactacgtg gtatggaaat gaaaattgat ttgaaccgaa
    17341 aattgattac tggtgtaaaa gctgatgaaa atggttctaa aggtcgtcga atgaatggga
    17401 ttttgaactt gatcaattca gcaaataagg cagaaacagc cactgcgggt gcagtaacaa
    17461 gaaaagatat cgatacttta tttaaattga tgtatgaaaa aggttatatg ggagaaaaac
    17521 tatgcttgat ttctccagat atgcaggagt taatgactga tgagttagat ggaaaatcaa
    17581 caaaaattgt tcagttcggg gaaagagtaa cttttggatt gcaaattgga aatatcgtgt
    17641 ctaattacgg tacaggtatt gctctactag aaccatcatt gccaaaagga acaattgccg
    17701 caatcgatac taattatgtg aaactacgtc cattacgtga atggagagca gaagaacttg
    17761 caaaaacaac ggattctaga cgtatcggtc ttgtaggtga atactctctt gaatacaatg
    17821 cttcaaactc tggggcaatt ttaaatttaa agtctgaata aaagggagtt agtactccct
    17881 tttttgatag gaggaattac agtggtaaaa aaagatgaaa ctaaaaaaga tgaagtcgtg
    17941 aaatatagag taggtaaaac taaaaatttt gttgggtttg ttcatcctaa aactcgtaga
    18001 tttatcacag cagattcaaa taacgaattt atcatttcta tagatgataa agaagcaatt
    18061 gcaattttgg aagatgcaat tgatgttaat gaaatttagg aagtgatctg atggatgaat
    18121 cgctaaaaac ggaaatcatt gagtctacaa aagaagattt tccagatttg agtgaagaac
    18181 gcataactaa tttattagaa ataattttgc tagaaattga atcatacaac acttgtaaaa
    18241 atgatatttc atgggaaaag ttaaaaagcg tgattagtga agtgttgtat aaaataataa
    18301 aaaatgaatc agaaaaaaca gtatcttcag ttagacgtgg cgatacaacg attagttatg
    18361 cttcaacaac aaatgatgtt agtgagttac ttctaggcta tggtgatttg atacgaagag
    18421 ttattggttg tggaggattg gagttttttt aatgaatgaa gcagatattt tagaaactac
    18481 ttatgaagat agttgtatta ttgaaagact gacggacatt gaagattcta atacaaatat
    18541 tactattcaa gattacaaaa aagtatatga taatcctatt tcttgcgctc tttcacaagg
    18601 tcaaatcgat ggactagcag tcatagaaga tggagagatg gtaaatgttt caactgacac
    18661 atataaatta tttgttcatc ctaagattaa actaaaaaaa ggagatcgaa taacaataac
    18721 tcaaaaggcc agtggcttaa ttttttctct atttgctact aagccttttt actatcctag
    18781 tcattgtgaa gtgaatttga taggaagtga gaaaaatggg taatctcaaa tatgaatcaa
    18841 atgcagaaaa gatcattgag aattttaaaa atatgactgt aattgctcaa aaagaaggaa
    18901 tatccttcgt taacgattca atgaataaag tcgttagttt agctaaacca ttaactcctg
    18961 taaaatcagg taatttaagg cgaggatatc gtgtagtaaa agctagaaaa ctatcaagcg
    19021 gtcgaattgt tggagcagtt ataaacaatg aacattattt taaatatgta gaagaaggtc
    19081 ataggactaa aaatggtggc tttgtcaaag ggaaattcat gttaactcgt gcaacgaatc
    19141 ttgcaaatat gtcttatatt cctcgaagat ttaaacaaat ggcaataaaa atcgttaaga
    19201 aaggaaagta acatgaaaga tgaaatcatt gctgcaatca gtagcaaatt aaaagaaatc
    19261 tatcctgatg ggacaatata tcttgattca gttatgcagt caactaaaga tttttatttc
    19321 gttttatcag taatggaatc tggaactgaa aatgtaggaa ttgatattca aaatgtttct
    19381 ttcttaattg atattgcatt gattgataat aaacctaata gaaatttaat taatgaatta
    19441 gtctcacgtt gtgggacttt ttttaatacg attacaatcg atgagcaaat attatttcca
    19501 aaagcctatt tacctgatga agcagatggt gttcaacata ttcgttttac attagaattt
    19561 ccacaatata ttgaatggag tgaaagataa atgggagaaa aaagaagtaa aaccggaatt
    19621 atttctgtcg aaaaacctac ttggtttcct ttggaagatg agacagggaa ttttccggta
    19681 tatggtactg cgttgccaat gggaacagca gtaagtatca aaccaacagc taattatgaa
    19741 acaacacaag attatgggga ttcagtggtt caagatcaat ttacagcttt tggtggtgca
    19801 gaagttgaac tggaagcaaa tggatatact cataaagttt tatctacaat tactggaggg
    19861 aaaattgtta aaggtggtgc cttgcgttct ggagaagata ttgcgcaaga tggtgcgttt
    19921 gcatatagac gtaaaaaatc gaatgggaaa taccgttata cggtctttta taaaggacag
    19981 tttgctctag attcagatga aacatcaacg attgaaggaa gtaaagtaag ttttactcat
    20041 ccaacttgga agggttcatt tgtagatgta ccaggacttg gatatatgta ttcagttgat
    20101 gaagatgatg aaggcgtgga taaagcaatg atcggaaatt ggtttacaaa agtagcaatt
    20161 ccaattgaag aagcagaact ttcaggaggt acagaataat gtcaaaatat caaacaacga
    20221 ttaaattaac gaaaaaagat gaagaaggta aatatgagca agtacaattt aaatctgctg
    20281 aatttctacc aggcccagtc gtagaagaag cagcagaagt aatggaggga atgcaaaatg
    20341 ctgtagatgg aaaatcagta tcagaagcat taagtcgagc ttattcattt attgcagata
    20401 ttttgtttga aggacagttt actggtgaag attatcgtca agggatcgat gctcgagaaa
    20461 ttgcttcgtt gacaggtaag ttattaaaat ctgttactgc aggctttgat gaaacttata
    20521 cagaaacgaa aaaaaagtaa gcgaagctct caaatatcct ttttttaaac attcgattac
    20581 ttatcgagaa ttagatttaa agacgcaatt actcgaagca ggttggacgt taccagaaat
    20641 tgaacgtact gactttgacg agttaatgcg tctttttgct tttcgagatg cagtaaaaga
    20701 gcatgaggat gttgagtact acgataactt cacccaattt taggaggtga tactttgaat
    20761 aacgatgacc taattctgaa gatgatacta gatgaatctg gttttactgc cggtatgaat
    20821 aatgctgtca aaaagttgaa ctcttttgat gaaacaatcg aaagaaaaag tagaaatagt
    20881 ggcagttcat taggtagtat ttggaaaata tttgctggaa gttttctagc tagcggagta
    20941 actagaatcg taggagctgg ctttgatcta atcaagggtt ccataagtgg agcggttgat
    21001 cgagtagata cgatgaataa cgctctacga aatttccaaa atatgggatt tagtaattca
    21061 gaaattatga agaatatcgg aaagaatggg cttttatctc aaggtattca aggacttcct
    21121 actgctttga atgatgcgat aagtcatgtt caacttcttg cttcttctac aggtgattta
    21181 actcgttcaa ctcaaatatt taaagcttta aatgatggaa ttcttggttt cggtggttca
    21241 actgatcaag ttaatgaagc tgttattcaa ttatctcaaa gtttctcaaa cggaaaagta
    21301 gacgcacaaa cttggaactc aatgatcaac gctcaacttg gacctactct ttctgctatt
    21361 gctaaaaaga tgggaattac aatgggagat ctgaaagaag gtttatctca aggtaagatt
    21421 tctgttgaag aattccaaaa tcaattaata gaaatggata ccaaaggtgg cggaggactt
    21481 aaatcattaa gtcaaatcgc taaagattct actaagggaa ttaaaacctc tatacaaaat
    21541 gcaaaaacag ctgttacacg tggtgttggt gaggtaatag aaggattaaa taaagcatta
    21601 gtagattcag atttaggtgg atttaaggga attattgata aagtgggcag ttcaatggaa
    21661 tcgttcttaa aagtaattgc tgcaaatatt cctacagcag tatcattttt aagtaattta
    21721 tttaacgaaa ttcaaaaatt tggttctgct ttgaaattca tgatgccctt tcttgttcct
    21781 gcagctaccg cttttggagc gttcatgttt caacttaaag gtataccagc aataataaaa
    21841 agcttcaata attttaagaa tgccataatt ggcgttggaa attcattaaa gattatgggg
    21901 gcaatagctg ctgctaattc atttgtttta attattggtg cagtcgttgg tgcaatagct
    21961 gttttcggtt actttatggc aaccaatgaa gagcttagaa acaaggttat atctatttgg
    22021 aatgatgtga aagattccgt aattggtgta ttaaaaaata taaaggactg gggaattgat
    22081 acttggaatt ctgctaaaga aatggcatca aatgcagttg agggtgtcaa agatgcttgg
    22141 tcaggaacaa aggaatggtt ttctaacacc tggaaaggta ttaaggatgg ggccacaggt
    22201 ttatttgata aaacagtaga gacttcaaga aatgcagtcg atagtgtaaa aaatgcatgg
    22261 tctaatatga agaaatggtt ttctgatact tggcaaagta taaaagattc agccagagaa
    22321 aaatggaatg gaatcaaagg ttctattatg tcagttgctg gtccattgat tacaggtata
    22381 aagaatgcat ttttacatgt tactttttat ctagagacct tatggaataa tttagttgag
    22441 attggtaaaa acgtctttga gattttaaaa aatgttatac ttgcgccggt tttgtttatt
    22501 acctccatga tttctggtgg atgggaagaa acaaaaaata atatgattgc tgtttggaat
    22561 aatattaaag aaagtgccat aaatatttgg gaatctataa agaatatatt tgtaagttat
    22621 tttacaaata tttattttgc tgcactcaat atttggacag ggttcaagct tacattgata
    22681 aatatttgga atgaagtggt aaatcaagct aaatcgattt ggttaaacat aaagtatttt
    22741 tttattaatc tttggattga tataaaatat tttgcaattc agaaatggat tgaattgaag
    22801 ttttctatta ttcaaacttg gatcgattta
//
LOCUS       DAYYCW010000044        22774 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000044 DAYYCW010000000
VERSION     DAYYCW010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.044"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..3433
                     /locus_tag="ACHAYA_001504"
     CDS             155..3433
                     /locus_tag="ACHAYA_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM8605305.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3974..5881)
                     /locus_tag="ACHAYA_001505"
     CDS             complement(3974..5881)
                     /locus_tag="ACHAYA_001505"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM8605306.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6011..6886
                     /locus_tag="ACHAYA_001506"
     CDS             6011..6886
                     /locus_tag="ACHAYA_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM8605307.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6966..7940
                     /locus_tag="ACHAYA_001507"
     CDS             6966..7940
                     /locus_tag="ACHAYA_001507"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8605308.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8120..9265
                     /locus_tag="ACHAYA_001508"
     CDS             8120..9265
                     /locus_tag="ACHAYA_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8605309.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9402..9746
                     /locus_tag="ACHAYA_001509"
     CDS             9402..9746
                     /locus_tag="ACHAYA_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM8605310.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10017..11303
                     /gene="efmA"
                     /locus_tag="ACHAYA_001510"
     CDS             10017..11303
                     /gene="efmA"
                     /locus_tag="ACHAYA_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM8605311.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11441..12040)
                     /locus_tag="ACHAYA_001511"
     CDS             complement(11441..12040)
                     /locus_tag="ACHAYA_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8605312.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12444..13565
                     /gene="mnmA"
                     /locus_tag="ACHAYA_001512"
     CDS             12444..13565
                     /gene="mnmA"
                     /locus_tag="ACHAYA_001512"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM8605313.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13811..15232
                     /gene="arcD"
                     /locus_tag="ACHAYA_001513"
     CDS             13811..15232
                     /gene="arcD"
                     /locus_tag="ACHAYA_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM8605314.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15246..16364
                     /locus_tag="ACHAYA_001514"
     CDS             15246..16364
                     /locus_tag="ACHAYA_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM8605315.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16424..17716)
                     /locus_tag="ACHAYA_001515"
     CDS             complement(16424..17716)
                     /locus_tag="ACHAYA_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8605316.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17866..18426
                     /locus_tag="ACHAYA_001516"
     CDS             17866..18426
                     /locus_tag="ACHAYA_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8605317.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18555..18932
                     /locus_tag="ACHAYA_001517"
     CDS             18555..18932
                     /locus_tag="ACHAYA_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM8605318.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18865..20937
                     /locus_tag="ACHAYA_001518"
     CDS             18865..20937
                     /locus_tag="ACHAYA_001518"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8605319.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21148..22095)
                     /locus_tag="ACHAYA_001519"
     CDS             complement(21148..22095)
                     /locus_tag="ACHAYA_001519"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8605320.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22341..>22774
                     /locus_tag="ACHAYA_001520"
     CDS             22341..>22774
                     /locus_tag="ACHAYA_001520"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM8605321.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         7376 a         4152 c         4817 g         6429 t
ORIGIN      
        1 tacgttaata aatctcttta ttatcaacaa cttaagttat attttttctt tttataaaat
       61 acacttatta taattttgtt ttttattgac agtaaaaata aattaggtaa tactttaagt
      121 aaacgctttc taatttgtta cgggaggaat acacttgtta aaaaaacgta taaaatacag
      181 tgcgctaatg atgaatctcc ttatgctcag taccccggtt ttaatttctg tcgttgaagt
      241 tgctgctaac gaacaacagt ctgtgaacga agaaaatgca cacgtgaaca ttgaagggat
      301 gtcaacaatg gaggaaatac caagaactag tgaagatatg gatacggaag aaggaaatga
      361 tcctatgatg aaagaatcag aaaatgaaaa gacaaaaaag caatctgaag aagcaaatga
      421 agagtggaaa ggggttgttt tcggagaaag cacaagtgct agtaggtatg ccattgaacc
      481 attagaagac ggcgtgcggc tctcttccac gaataacggc ggaaaattcc agtcaagcgg
      541 atcagatgga atgacctatt attatcaagc aatccccaaa gacatcaact ttaggatgcg
      601 ggcgacgatc gaaatagaat cttggaccta tacgaatgcc caagaaggat ttgctttaat
      661 ggtaagagac gcagtaccaa aaaatcattt gtttgggcag gcattttatt caaattcatt
      721 tgccatacta ggcagtcgga tcgaatatgt ctgggactcg gatagacaag aagtggtcaa
      781 cacgagcggc agcaaatata cgatgcgact tggattgggg acacgggcaa tcacaggaat
      841 atcatctgaa gatccgcaag ctgcgccagc tcctgggagt gtctcagtgg aaacaacacc
      901 tttagagact tcagcagggt tcttggaaaa agaaacaggt tcttataata tattcggaaa
      961 cggtcatggg aatctgtttc cggccacgaa ctcgattact aaactggacg tagagatgaa
     1021 aaaaacgaat actggtttag aggcatatta ttacgatcct gaaacaggag aagaaatggc
     1081 ttctgacatc atgtatgact gggaaaaatt atacgcttca gatgaatcgg ttatttatgc
     1141 aggatttgct gcagcaagga atatgacgat caaggtagaa ggaatcgaag ttgcttactc
     1201 agatcctgcg acagatcctc ctgggcaaga aagacctact cgcttgattg atccgattta
     1261 cactgtgacc tctagtaatc attcaggaaa ccaagatcat actatgtcct ttagagcaaa
     1321 tgctgacgga ttattgacaa ttaaaaataa agcaacagga gaggtgctta agaacgcaaa
     1381 aaacttggct atcactgcga atcgggaatt tgattatcag acaaaactga tggaaggaac
     1441 gaacgaattt actttcagtt ttacgccaga taaaaactat ccgccagaag aatacgtaga
     1501 gatgactagt tatgaaacaa aagagattct tcatacagtg gactaccgta aacctaaata
     1561 ttcgacagtt tatgtaacac cagaagggtc agatgcagga aacggaagca gacaaaatcc
     1621 attatctctg acacaagcat tgagatacgc atatgctggg cagacaatcg tcatggcgcc
     1681 tggaacttat tccttcgaaa gaggattgac gattccaaaa ggcgttaacg ggacaagaga
     1741 aaacccgatt cagttgattg cagagaaaaa tccagaaaac aagcgtcctg tacttgattt
     1801 taaaggaact ggaaatggga tgacactgtt cagtcactac tggggtttac gagggtttga
     1861 cattacaaat agtgcagcta tgcaaaaagg attacagatt tctggaaatc ataatttgat
     1921 cgaagagatt catgcttatc ggaatggaaa tacaggaatc caaatcagcg gtagctcaaa
     1981 tgatcctttc gaagcatggc ccgcgcataa cttagtcaag aactgtacat cttttgaaaa
     2041 tgccgatcca ggatttgaag atgccgatgg atttgcggcc aaactgacaa taggagaagg
     2101 aaatgtattt gacggttgta tcgcgtatca taatgcagat gacggttggg acctttttgc
     2161 taaaaatgaa acaggttcga tcggaaaagt aatcatcaaa aactcagttg cttatcgaaa
     2221 cggctgggta ccaggaattg agggagaagg taacggtaat ggattcaaaa tgggcggttc
     2281 ttctttaacg ggccctcatg aattgatcaa tagtttgtct ttcgaaaatc ttgcgaaagg
     2341 aatcgattcc aatagcggaa cagatattat cgtcaatagc agtacaagtt ttaataacgg
     2401 ttcatataat gtggctcttt atacctcttc agcaggaaac acagactacc atgcttctgg
     2461 tatcttatct ttccgaacag agaatcttga gatgagagag cagatccgcc cattgaatca
     2521 aaacgaagat caaatctacc actcgttgaa ctactattgg aatgaacgag agaaacaatc
     2581 agaaaatact cttggacata ccatatcaaa agattggatc gagtctctaa atacatgttc
     2641 gaaagaaggt cagcagcctg ttacgcgcta tgaaaatgga agtatccatg ttcatggact
     2701 gatgcagatt aaaccgggga atcgtcaaac agaaaaggaa agagtggatg gtcttccatt
     2761 atctgtaggt gctatttttg acggagaaac ctcaccgtct tcggaatatc cagagtatgc
     2821 aatcgtacaa gaaccaaatc agacaaaacc aaatgaacaa aataatccga ttattataca
     2881 agaagcaaat ggtgtggcgg agaatgcaga aggaaatatc attgaactga agccttttac
     2941 agtttttgtg tcaactttca caccaaatca aggaacgcct gtctttgaag atatgcgtta
     3001 tttcccagaa aagatccaaa tcgttgaaac acagtcaagt gctcaatttt ctaatcatac
     3061 cgctattttt tcagcacctt caaaaggaga atatacgcta gaagtcactt atcgttcgga
     3121 aatatttgat ggggaaaaat ggtctgctca tacagaatct gttacccagc aaaaagcgat
     3181 cactgtgcga gaaactgccg atgtgccccc gggaaatgac aaaccagaaa gagaaaatcc
     3241 aggaaatgaa tcaccaaatg gaaaaccaaa tggaaaacct tctcatccgg ataggacagc
     3301 aaaagaaagt gaaaaaaatg gcactcaagg actcccaaaa acgggagaat ttaataaccc
     3361 gttgctagca ttagctggag gaattttgtt aatcggtgta gttgtttatg tcatgaaaca
     3421 gcgaaaaaaa taagtggaat ttctcctttt atgatgtaaa acaattgact gaggaaagta
     3481 aaaatagaag gaaatgagac caatagatgt ctcacttcct tctatctttt tatagatgag
     3541 gttctccttg ctgcttttga ttggctgttc ttattacttg atgcaggaca gctttttcac
     3601 aatctctcca ctaaacggtg ttcaatcagc tgaccctcgg tattagctca acaaacggca
     3661 aaatatgagg aactattttt ccgttttttt ctctaatact caggtcagga cgctgatttc
     3721 acaacctctc gataaacggt gttcaaaagc cagtttctcg gtagtaagtc aaatttatgc
     3781 aaaaatgtgg aaagccattt tcacaaaatt tttcttacta cttgaaacta agcagctttt
     3841 tcacaacctc taatacatgg tattccagaa gtaacttctt cgaaaaataa gccgaaatgt
     3901 cacaaaaatt tgagaagcga ttttcccaaa ttccttctta tttcttgaag ctgaccactt
     3961 ctggcacaac ctcttaccag attcgcactc ttttttctgg cgctagatac atagcgtctt
     4021 gttccgtgat accaaatgtt tcatagaatt cgtcaaggtt ttttggttgc acgtttgctc
     4081 ggagtttagc aggggcatgg acatcgattt gcagtaaaag ttgttggtat tctttttttg
     4141 cttttgtacg ccaaatcgtt gcccaattga caaagaattc ctcgatagaa agatctgatt
     4201 ccgtttttgc tgcttccaat gcacagctta gaccgcctgc atcagcaata ttttccgaaa
     4261 cggttaattt tccgtttact tttccgtcag caaaaggcag accatcgaat tcttcgatca
     4321 ttgcttgtgc tttgttttga aattgtgtaa gatcttcttc tgtccaccag ttgttcaagt
     4381 tgccatattc gtcaaataaa gcgccgttat tatcaaatgc atgggaaatc tcatgagcga
     4441 taacagctcc gattccacca taattagcag agcttgattg ttctagactg taaaatggtg
     4501 cttgaaggat cgctgccgga aagacgatca cattccgaaa tggatgataa taagcattga
     4561 ccgtgtcagc actcatttcc cattctgaac gatccactgg tttgttccat ttgctgaaac
     4621 gatcttttaa tgtgagtttg ctgaaaacaa gagtatttga aagcaaagta ccaccctgat
     4681 ctgcaggtac tgttttgaac tggtcgaata atgctgggat cttatcgggg tagcctactt
     4741 ggatacctaa gttatttaat tttatgacgg ctttttcttt tgttgcatcc ccaagccagt
     4801 cattttcttc tagacgtttt ttataaacgg cgatcatttt tttcaccatt tgttctacgt
     4861 cttttttagc agcttcgcca aaatattttt ttccgtagta atcgccaact acttgatcga
     4921 attgtccaga ggcaaggtaa tacgctgctt ttttctgagg cattgcttcg tctgttccag
     4981 acaatacgcg agaatagatt ccgctgactt ggcggaattc ttctgataga taaccactaa
     5041 gagaacgtac tgttttaatg atcatccagc ttttgaataa cggaaaatga gcttctgtca
     5101 ggatgtcatt caaatgatcg aagtacactg gatcagttac gatcacttta tctggagttg
     5161 tcccaatcaa acccgtgaca agttttttca agtccagttt atctgtgtat ttaataaatg
     5221 tatcaaaaga ttgcggatta tacattttgc tgtaatctgc gttttcttct gcacttttta
     5281 catgaggagc aatcaattta tcaaattgca ctgcttgttc cgtgattgct tctgcctgac
     5341 ttttttcttt gccggcaagc tctaataact gaacagccat atcaaagaaa acgcctatta
     5401 gttgtgcacc gttgggatgg tctgatgcat aatacgtctt atctggaagg aataaagaag
     5461 gcggataagc aaagaaggcg ttcgtttgcg cattcttcat atctgcatct acatccagat
     5521 taaatggcaa tggtaatgta tccaacagcc attctggaaa ttgcttattc aattcgtcga
     5581 aagactgcaa tgattcaact ttttcgatta taggttgaag cggttgagca tgcaactcat
     5641 ttcttcggtc ataatcgttt gccagttgat aatatgcaag gaaatgtttc attttttctg
     5701 aagacacttt ttctggatgg gcaagcagat cgtctgtttc tcccatcaaa agttggtcga
     5761 tcccgtcaac taaatcttgg aaaccgcctg tagctggttt atctgcaggg atttttgctg
     5821 ttttttgcca ttcttcattg actgcttcat agaaatcttg tttcaatcgt tctttgttca
     5881 ttcatcgatc gctccttttt tgtttcgtgt gcccattatc ttacaaaatg aaggaattaa
     5941 caaatagaac ataaaaaatt gaaaattcga acaatcaaac tgatttgttt ttttcaatcc
     6001 ttcacttata atgaaaaaaa ggaggcgatc ccaactgaat caagtagtgg ataaaccttt
     6061 atatttcaaa gtagataaaa aaattaaaaa attagccagc actcagcagc tgcagtcaag
     6121 aaaaagtaaa ttgctttttt tagctctggt ttttgaagat caaagttatg tgattatcga
     6181 tcaatcagga catccggtag aatattctcc agcaaaatat acttatcaag agggtctttc
     6241 ttgtaaaaaa tggaagctga tcaatgaaga gccaatcgag ttatctagat ggatcaacag
     6301 aaaagaagat attcccgttt tgatcgaaga aaaaaggtca ggaaaggaac tcgcaaattg
     6361 ctgggtggga ttgccagaag aacgtttttt gaggtataag aaatgggcta ccccctcagg
     6421 ctatctttgt ggtacttacg cggcagcagt tctccttgcc tattatcaag actatcgtaa
     6481 ggggtggatg cttccaaatg agattcgcaa gaaaaatacg gctgattcgt tggttttgac
     6541 gaaggcattg cgtagccaga tccaaccact aggacttcct acgattcctt ttcaggttag
     6601 tacagggatt tctagttttt taaagaagaa tggcaatcat gagcgtgcac gagcaactct
     6661 tctaggttca tggcagcgag ctacaaaacg tatacgtgaa ggcaaacctg tgatgattgg
     6721 tattttgaaa gtacttggca gtacatatgg caatcattgg gtgacagcgt acgcttattt
     6781 tgaaactgaa acaggagaac gttattacaa agtacacgac aactggggcg attatcataa
     6841 agtcattcct gccagttgga gtaacggaac agtttcactt ccttaagaat ttgctgaaat
     6901 taacagaaaa tcttggaaaa atatgttaaa ctatcgaaga atagagaagt tttggggagg
     6961 actacatgag taataagtat caggatgaca cgttgaagat ttttagtttg aatggcaacc
     7021 gtccattagc tgaaaaaatc gcgaaagtgt tcggaacaga attaggtaaa agcgtcgtga
     7081 agcaatttag tgatggcgag atttccatca atatcgaaga aagtattcga ggggatcatg
     7141 tgtatatcgt tcaatcaacg aatgcacctg tcaatgatta ctatatggaa ctactgatca
     7201 tgatcgatgc aatgaagcga gcaagtgcga aaacgatcaa tgtcgtattg ccttactatg
     7261 ggtatgctag gcaagaccgc acggcgaaac ctcatgaacc gatcactgcg aaattgatcg
     7321 ctaacttgat cgaagaagca ggggcaactc gagtactgac tttagatctg cacacggttc
     7381 aagtacaagg attctttgat attcctgtag ataatttatt tacgatgccg ttgtttgctc
     7441 attattaccg tcaattaggt ctgacgggag atgacatcgt agttgtttcg ccaaaaaaca
     7501 gcggtgttca gcgtgcacgt agtctttcag aatatttgaa cagcacatta gccatcgtag
     7561 atcatgcaga tgatgcttca actgagggtg gttatgtgat cggtgatgtt caaggaaaga
     7621 catgtatcat ggtagatgat atcttgaaca caggagcaac gcttgcacga gcagcaaatg
     7681 tattaaaaga aaacggcgca aaagaagtat atgcctgtgc gtcacatgga ttattgtccg
     7741 atcatgcgaa aagtatttta gacaatgcac caatcaaaga tatttgtatc actgattctg
     7801 ttttcacaga agaaaatcgt catccagaaa acttaacaat cgttacatgt tcagaattaa
     7861 tgggtgaagc ggtcaaacgc atccatgaaa atacaccgat gagtccgtta ttccgtctgg
     7921 aagaaaaaga attcgaataa acaaaaaaaa gagggaaaga agttgtgaca gaagtgagca
     7981 gcttcaagga gctgcgacaa gacttttgtc acagctcctt ttttctatgc ccatgaaacg
     8041 tcctctagac aaaaatgagc tcttctttgg tttgttcgct attttatgct agaatgtgcc
     8101 tagcaataat ttggaaggga tgaatgaagt gaaaactatt tatctggatc atgcggcaac
     8161 tacccccatg catccacaag taattgaagc gatgacggca atcatgcaag acacgtttgg
     8221 aaatccttca agcatacatg gatttggtcg ccacgcgcat gaaaaattgg aaacagcaag
     8281 acaaatcatt gcagacagtc tgcaagcaaa acctcacgag atcatcttta acagtggggg
     8341 aacagaaggg gataatacag ctatcttaga aacagcgttc tcgcgtcaaa aagaaggcag
     8401 acatctaatc actacagcta tcgaacatcc tgctgttctt aataccatga aatatttaga
     8461 gacaaagggg tttgacgtta catatctgcc tgtagatgaa aaagggaatc tgtcgatcga
     8521 agaggtcaaa aaagcgttac gagaagatac gatcttggtc tctgtgatgt acgggaacaa
     8581 tgaaatcgga aatttgatgc caatcaaaga aatcggtgaa cttttagcgg atcatccagc
     8641 ttatttccat acagatgcgg tacaagcgta cggaaaccaa acgatccatc cagaagaatt
     8701 aggaatcgat ttattaagta tctctgctca taaaatcaat gggccaaaag gcgtcggttt
     8761 cttatataaa cgagacggag taaatatccc tgcattgctt ttaggcgggg agcaggaaga
     8821 aaagcggcgt gcaggtacag aaaatttagc agggatctgg ggtatggcaa aagcagttga
     8881 actgctgtca cctgaagaac gtgaagcacg taacaacaaa tataaaggat tccagcaatt
     8941 gattcttgat acattagaag caaacgaaat cccttatcaa gtaaatggtg atttaacgaa
     9001 taaactccct catgtgttga atcttcgctt aattggcgaa gcaaatgatt tgctactgat
     9061 gaagcttgat cttaatgggg tcgctatttc tactggttca gcttgtacag caggaaatat
     9121 cgagccttca cacgtattag aagcaatgta tggagaagat acaccgatac taaaagaatc
     9181 catccgtatc agctttggtt tgggaaatac cgaagacgac atccagtatt tccttgatca
     9241 attagttcgt gctgtcaaaa aataactagt gaagtcattc ttatatgaac agatgtctga
     9301 gagaacgcct tcatttttta gggaacttaa ctgaaaaacc tagtaaaaat caggataaac
     9361 ttttataata aagagaaact aattttatcg aggtgaaaat catggcattt gaaacaacag
     9421 caacggtact gggcgcacca attagctatc gtttgcatcc tgatgcaaaa agatatacat
     9481 tgcgcgataa tggatttaca gaaacaaacg gaggcaatta tcaattgatc cgaccattag
     9541 atgccacgcc gcaaagcaaa gaaggattca aattaaaaat cactgtatca aaagatattc
     9601 aaacattgaa aatgtcgatc actacagcaa atggattgaa agcagtcaat attttcaaag
     9661 atccaaaaca gaaaatgagc caagataaat tttattttct gatggatggg atgattagcc
     9721 gtgggttatt cgaaaaagtc gaatgatctg tggatataaa aagacaacta gttttgaaac
     9781 ggcctattgt tagatgacta actttgtaag gacgacgcaa attagttgtc ttttttattt
     9841 ttaaatttaa cacatgttgt tatttttaat attttgctga tttattgttt ctaagtaaag
     9901 aaatttaaat tgaaacgatt gttatttagg gaaaaaggta ttttttgaaa aaagaatccc
     9961 gtataatgat tgttgtgcaa acgctataca aaatgaaaaa gaaaagaggg atgtttatgg
    10021 aaaatgaaca gtcggtcgtt ttaacgaatt ggaagagaaa ttatttgttc tttttatcgg
    10081 gacagttttt gtcaggaatc actagtatgg tcgtccaata tgcgatcatc tggtatttga
    10141 caagagaaac cggttcggcg acaattttga gttttgctac tttactagga atgattccca
    10201 tggttttact aagcccattt gtaggaccgc tagtggatcg ctgggataaa aaagctcttt
    10261 tgattgtcac ggatattatt gtagcgattt tcgcgcttat tttagcagta gtcggaacga
    10321 tttcagaatc cttcccgatt tggctagttt ttgtgtcttt gtttatgcgt tcggtcgcac
    10381 aaacattcca aatgccaacg atccagtcga ttatgccgac aatcgttcca tcttctcaca
    10441 taacaaggac gaatggacag ttaggaatgg tccaatcagc gaatttcatc attgcgccag
    10501 ctctaggtgc ggctttgttt tcagtcgttc ctgtcaatta ccttatttta ttagatgtat
    10561 tgggggcagt ttttggtgtc ggcttattga tttttgtgaa gatcccaaaa gtttctcctg
    10621 aaatattgga agttccgctt actattttta aagatgcaaa atttgggctg cagcaattga
    10681 tggataataa agggttatgg tacataacga tcaatggtgc atttgtgatg ctgttgttca
    10741 tgccagcaat cagtctgtat ccattgatga cgctagatta ctttggtggt tccgttggac
    10801 aggcgggagc agtggaagta gtctacgcag tcggaatgct cttaggcggt gcactgatca
    10861 gttttatagg aacatggaaa gaccgaatga aacctattat tatagcctac atcattatgg
    10921 gcctgacgat cggtgcaagc ggattggttc caaacgatag tcaagggttc ttgtactttc
    10981 ttatcttgaa tgcaggagca ggttgtgcaa cgccatattt caatacactg ctgatggcaa
    11041 tgatccagca gagttatgaa tcgaatgttt taggtcgtgt cttagggaac ttcaactctt
    11101 tgatgaacct ggcaggtcca attggattat tatttgcagg accattagct gatcgtttag
    11161 gggtagaaaa aatgttcttg ttttccggaa tcgggatttt attatgcgga atcgttttat
    11221 ttttaacgtc agctgcccga aattatgata aagaattaca gaaaaaactg gtaaaagaac
    11281 accatgagca aaaagacgaa tagtatggaa tggagttgtg acagaagtct tgttacaact
    11341 ccatttctga tcctaatgtt cctgtggcaa taagcctact caaatgaaaa atatgagtaa
    11401 ttatctttat ttgtttaata cttgatatag ggcagttttt tcacgaccta tcgtacttcc
    11461 tctttgcttc atagatttct ttgatttgtt gctcagataa cggagtgcca aatggtagtt
    11521 tttcctttaa gagttctctt tccggtgcgt ctacgcctac attgacatcg ttctcgatag
    11581 gtacgctgta atgatggata tgtccatgaa gattgcggat agtgtcgtta ggcccgagca
    11641 acatcggata atgagtcaag taatattgtt gatggtcgaa tttaacgatg gcacctacat
    11701 cataaaaata aaatttggga cttccgtcag taagcctagg atcatttttt tctaaataac
    11761 ggaaaaaagc gcgactgtca tggttccctt tgatcaagtg gattgttcct tgcagttgag
    11821 tgaccagttc caaaatttct gcatttgctt tttcatattg cggatgcata gcaaggtcgc
    11881 ctagatgata aacatggtcg gtttctttta ttactgcatt ccagctagtg atcaactgtt
    11941 ggttcatttc atccacattt gcaaagagcc gcggagcaaa gtcgtttttt cctaataagc
    12001 gttcatggaa aaagtgcata tcactgataa agtaattcat gtatcttttg cctccctttt
    12061 ttcttagagt aaacaaaatc acttatctga gcaactcata acatctaaaa agaaaaggtt
    12121 gtaaatgaga cgtgcagctt taagcgataa gaagcaattt tgaaaaatag tttttcacaa
    12181 tttataaaat tattccttat tgccgaaagg ctggcccttt ttatgagtat gccaattttt
    12241 tatatcgaat gctcaaaaga tgagtgattt tttggttgac tatcatgttt gtgtactgaa
    12301 taggtccagt agaacagtat ttttacgact tgataattaa gggatgaaat tttcagaaat
    12361 atgacgtata atgtaaaata ctgaaaagtt gcgaccttca actcgtagat gtttcagaat
    12421 ctaattgaaa tgatggtgat atcatgacag acaacagcaa aacacgcgtt gtcgtaggga
    12481 tgagcggcgg tgtcgactca tctgtaacag cacttttgtt aaaggaacaa ggatacgacg
    12541 tgatcggaat attcatgaaa aattgggatg acacggacga aaacggtgta tgcacggcca
    12601 cggaagatta taaggatgta gcgaaagtag ctgctcaaat cgggattcct tattactctg
    12661 tgaattttga aaaagaatat tgggatcgtg tatttgaata tttcctagcc gaataccgcg
    12721 caggacgtac acctaaccca gacgtgatgt gcaacaaaga aatcaaattc aaagcattct
    12781 tggattatgc aatggatctt ggcgccgatt atgtagctac tggacattat gcgcaagtca
    12841 ctcgcgatga aaacggcgtg gtccacatgc ttcgtggagt agacaataat aaagatcaaa
    12901 cttacttcct tagccaatta tcacaagaac agctttcaaa aacgatgttc cctctaggcg
    12961 gcatggaaaa atcagaagta cgggcaattg ctgaacgcgc aggccttgcg actgctaaga
    13021 aaaaagattc aacgggaatc tgctttattg gtgaaaagaa cttcaaacaa tttttgagca
    13081 actatctgcc agcaaaaaaa ggaaatatgg tcactcttga tggtgaagtc aaaggacaac
    13141 atgctggctt gatgtattat acgattggtc aacgtcaagg actagggatc ggtggcggag
    13201 gagattcgca agaaccctgg tttgttgtag gaaaagatct agcgacaaac acattatatg
    13261 tggggcaagg gttccatcat cctgcattgt atgccacaag cttggatgca agtgaaatcc
    13321 actttacgac gaatgaacca atgccaaaag aattcaagtg tacagcgaaa ttccgttatc
    13381 gtcagcaaga cgttcctgtg acagttcgct tattggatga taatcgtgca gaagtcgtat
    13441 tcgacgaacc tgtacgtgca atcactcctg gacaagcagt tgtattttat gatggaatgg
    13501 aatgtctggg tggtggtttg atcgaccacg catatcaaga aacaaaagtt ttacaatacg
    13561 tttaagcgat attaaatgaa tcaaaacagc tatggctaac aaaagaaaca ccttttcttt
    13621 tggatagcat agctgttttt ttgcaataaa attaattttt attcatttta tacttgaaat
    13681 acttgtgtaa aaagttcata atatccaata atatgaataa agttgctttt tgttaagcaa
    13741 gagcagttgc aaaatagaag ggaatctttt gcaagtgatt tgttcaattt tataagtgag
    13801 ggtgatggaa atggaaaaga aatcgacggg tattagcaaa tggggactgg ttgctctggt
    13861 tgtcagttcg tcaatcggaa gcggtgtgtt tgggatcaca agtgatctgg ctagttctgc
    13921 agctccaggg ccagcgatcc tttcatggat aatcgttggg attggtattt tagctttggt
    13981 attatcgctg aatcatttag gagaaaagcg tccagatcta gatggtggta ttttcggtta
    14041 cgcggaagct tcatttggta aattaggtgg cttcatcagt ggttggggtt actggttgtc
    14101 tgcgtggctg ggaaatgttg cttttgcaac gatgctgatg agcgccatgg gagaattttt
    14161 cccgatattc aaaggtggac aaaatgttcc ttctatttta tttgcaagta tttttatttg
    14221 gggtctgact ttattagtaa acaatggagt tgaaggagcg agcgtcatca atacaatcgt
    14281 gacgatctgt aaattagtgc cattattcct atttttaata tttgtattta tcgcatttaa
    14341 agtgaatata ttcactgctg atttttgggg taacgtatct gataatctag tgaatggaac
    14401 agggcagcaa ggaacgatct ggttgcagat taaaggttgt ttgatggtta tgatgtgggt
    14461 ctttgtaggg atcgaaggcg caagtgtttt agccaaccgc gcgaagaaac gttcagaagc
    14521 ccagcaagca tcgatcattg gtctgctttg tttattgttt atctatatcc ttgcttcgat
    14581 cttgccatac ggcgtcttat ctcaagaaga gttagcaact atttcacaac ctgctatggc
    14641 aaatatttta aaaggaatcg tgggaaattg gggagcagcg ctgatcaata tcggcttgat
    14701 tatttcgatc attggttctt ggttgtcttg gacgatgctc ccagcagaaa caacgatgtt
    14761 gatggctcga gacggtctgt tgccgaaagg ttggggaaaa acgaataaaa aaaatgcccc
    14821 aacttattcg ttagttgtta ctgcaatctt gactaatcta ttcttattga ctttcttagt
    14881 tactgattat gcctatcaat ttgcctattc tttatgtaca gcggcaattt tgatttgtta
    14941 tctacttgtc gggatctatc aagtccagtt ttctttaaaa gaaggaatca aaaaacaagt
    15001 agtgatcggt gggattgctg tagtttttga attgatgggc atcattttag caggattttc
    15061 ttatgtcctt ttatgtagta ttgcctatct tcctggtttt tgcttctact ggatcgcttg
    15121 tcgtgaaacc aaacatcaaa ttacgggaaa agaaaaagta atgattgggc tgatttgtgt
    15181 ggccgcactg ctttcaatcg ggttgcttgc tgtaggctgg atcaagatat aagggggaaa
    15241 gaaacatgaa aatcgcaaat ttcggtgtag aagaatggct aaataaatgg gaaaagaaag
    15301 cggtatatga tattgcacaa agctcgatcg aagcattgac attagacgaa ctcgttggct
    15361 tagatgggac tagcgtaagt aaatttttcg aaaaacgcaa aaacgaacca ttggactatg
    15421 gctggatcga aggatcagat gttttcaaac aagaagtcgc ttctctttat caaaccgtcg
    15481 atccggaaaa catcttacaa acgaatggag cgactggtgc taatctttta gctctatacg
    15541 cgttagtgga agcaggagac catgttgttt cgatgcttcc tacttatcag caattatatg
    15601 acatccccaa atctttaggt gccgctgttg actttgttca tttgaaggaa gaagaagatt
    15661 ggcagtttga tttagaacag ctagaagcct tagtcaaacc tgaaacaaaa atcatctgtt
    15721 tgaatagtgc aaacaatcct acaggcacat tgctagatcg gaaaacttta gaaaaaatcg
    15781 cagaaattgc tcgtacagta gatgcatatg tattgataga tgaagtatat gcgcctttaa
    15841 cggataaagg tgaattcttg tcgatcgtag atgtctatga aaaaggtata gcaaccaatt
    15901 ccctatccaa aacctactcc attccaggta ttcgtattgg ctggacagct actggacctg
    15961 agttggcaga agtttttcga aagtatcgag actacacgat gatttgcggc ggagtcctgt
    16021 cagatgatct tgctgtacat gcattgaaga ataaagaaaa aatattggcg cgaaacaaaa
    16081 agatcattac agagaacctt gtgattttaa aacaatgggt agccaatgaa ccgaaagttg
    16141 aacttgtcgc tcccaattac gtctctactt ctttcattaa attagccatc gaagaagatg
    16201 accagacatt ttgtatcaat ttattagaag aaacaggagt actattagta ccaggaagtg
    16261 cctttgattt accgaagcat gcccgactag gttactgttg taaaaaagaa accttggaaa
    16321 agggattatt cctcttatct gagttcttga aaaaacaggc ctaaacaaaa acactgcaag
    16381 gacagtctgt gacaactgtt caagcagtgt ttttcattta cgcttattgt ctatgagaag
    16441 gagagtgccc tgaagctatg gagactcctg aagctttttc ttctgcagtt tcttgctgat
    16501 ggatctcttg tgtattgtgt cgtacaagca agaagttgac caaaacaaga agagaagtcg
    16561 aaagaaagac gccgcgataa ccaaatgcac tggaaacact ggagccgatc ataggcccta
    16621 ctacgttacc agttgcttgg aaagattggt tataactgaa gatccgtcca gcagcttgat
    16681 gaggcgaata ttttgtgatc aatgcttgga cagcagggag cagacaagca tcagagatac
    16741 caatcaaaaa acgtaaggca gctaattgcc aaacattcgt gacgaatgcc attgggatat
    16801 aaaccagaat cgcaaaaccc aaaccaatcg caagtattct ttcactccca atcttgtcac
    16861 ctaagcgacc aaatcttgga gcggctatca aagtggcaat cccaggtatc gaagcaatga
    16921 ttccgctgat caacgtaaca ttcccatgtc catgcagcaa ttggcgaatg tacagactga
    16981 tgattggact aatggaattg ttggatgctt ggatgatcat cgttgtgacg aacataccga
    17041 tgacgacgtg tggatatttc aagtctttga aaatctgttt ggcagaaacc atgttttctt
    17101 ttttcactgg gacaaatttt tcatggacga aaaacagact caacaagaag acgaaaaata
    17161 agatgatccc tgtaatgaaa aaggtagggc gatagccaaa cgctgaagca gagatcccgc
    17221 caagaagcgg tccaagtaat gttcctgtaa cagaaccagt tgctaaggtt ccaagaactt
    17281 gaccactttt ttcttttggc gtcccagttg cgacaagggc agtggcatta ctgatataac
    17341 cagaaaaaat cccttgaagc agacgcaatg cgactaattg atacacacta gtcacgcaac
    17401 ccatcaaaga aatgacgatt gccattccta atgaagcacg caacagcatc aattttcttc
    17461 ctttttggtc ggctaatttt ccccaccaag gagagataag cgtagtcacc aagaaagtag
    17521 atgaaaaagt caatccgctc caaaagttca attgtgacgt actataatta cctaacgtat
    17581 ctatgtaaag tgacataaaa ggcatgacta aactaaatcc gataccagcc ataaatgttc
    17641 caaaccataa gacaaacaag ttttgttccc aagtttctct tttcctaaat aatttttcct
    17701 ttattcttgc agccaattag actccttctt tctgaaaatt ttcatctaca gctctttatt
    17761 ataaactaca atatgtagtt tatcaatgaa agtctctttt ttctctttct ttttcgtggt
    17821 aaactgaaag agtgaaggat ttattttatg agaggaaaca tggaaatgaa aaaaagaaat
    17881 ctgtctcaag agaaaatcat cgactgcttt cgagaactag cagaagagat ggatgttcag
    17941 caaataacct ttcagcattt agccaaacgc ttggatatca aggctccctc gctgtataac
    18001 catttcaaaa atattcgtga tgtcaaaaca gcgctaacag cgaaacttct gcaggaactt
    18061 aatgaccagc tgcgacgggc tcttgtggga aaaagtggtc aggaagcact gcagatctat
    18121 gcagaaactt atcgatcctt tgctttttca aatcaagcag tgtatgagtt actgatcagt
    18181 gtaccgcata ccaatgaagc tgttttatta gatggaatcc atgaaacgaa tcagatcatt
    18241 ttgcagatat ttgaggcttt tcaattaacg aaagaagaaa aagttcatcg aagcagggaa
    18301 cttcgtagta tgatccacgg ttatctttcc ttacgttttt taggatactt tacaaaagaa
    18361 ccaacaatcg atccagaaga cagctatttt tggatgatca ataactttat cgctactttg
    18421 ccttaaacaa tcagattcat ttgtaacaat tttaagatgt tcattaaatg acccttttat
    18481 aagagaaaat tcttgcaact ttcttgaaaa gtgactaaga tgagtctaga aaagctgaaa
    18541 gaaggagcac gagtatgtat caagtaatta ccatgttcgg ggacaatgag ccttggtggt
    18601 tttttgaaga ctgggaagaa gatattgaag aagaagaaac attcgagtct ttcgaagaag
    18661 cacgacaagc gtacgaaaaa caatggaacg aaatccatca aaattatgaa tatatcaatg
    18721 caaaaagtaa ctttctcagc gccttttgga atgacggaga tgagcgctgg tgtgaagagt
    18781 gtgacgatga tttacagcaa tacaagggac ttgcgttgtt gaaaaatcat cagcccatca
    18841 cagtagaaag cagaaaggaa ttttatgaaa caactaattc tagcggaaaa accaagcgtt
    18901 gcgaaagacc taagcaaggt gcttggtgct aatcaaaagc acaaaaatta ttatgaagga
    18961 cccaaggtga tcgttacttg ggcccttggt catttattag gattgaaaat gccggaagat
    19021 ctgaataagg aatggcagag ctggcaaatg gacacgttgc caatgatccc taaaaaacta
    19081 ggaatcaaac cacttcctaa aacaggacat caattaaaag caatcaagca gctggctcag
    19141 cgaaaagatg tctcagaagc agtcattgcg acagatgccg gaagagaagg ggaacttgtt
    19201 gctcgctgga tcttagagta tgtgcatttc aataaaccag tgaagcggtt atggatctct
    19261 tctcaaacag ataaagcaat caaaactgga ttcaagcaac taagacctgc gaaagaatat
    19321 gatgcattgt atgaatctgc tcttgcccgg gcaaaagcgg actggctggt aggattgaat
    19381 gtgaccagag ccttaactgt gaagtacgcg gataatttat cagcaggccg tgtgcagaca
    19441 ccgacactag cattagtgag agaccaagaa aagaaaatcg agcagttccg tccacaaacg
    19501 tatttcacga tcacgttgca agctggaaaa gaacaagcca aaatgcttca aaaaaatcaa
    19561 tttgctttaa aaacacaaga agaagcacaa aaacttgttc aattgatcag tacaagcaaa
    19621 ggaactgtca cagaagtcca agaaaaatta aaaacggaat ctgcgccatt gccttatgat
    19681 ttaacagaga tccaaagaga agccaatcaa cgctatggct attctgcaaa gaaaacatta
    19741 agtcttgtac aaagtttgta tgagacccat aagatcgtta cttatccgcg tacagacagc
    19801 aaatatctga caaatgacat gaaaagtacg atgaaagaac gattgcaagc agtcagcgac
    19861 tttgctcctg aggtcaagca gtatttgaaa aacggagcca tcgtccaaca gacaaaagta
    19921 ttccaagaca gtaaagtgac cgatcaccat gcacttttgc caactgaaaa cagagcacgt
    19981 tatgaaaaat taagtaatga agagcgaaaa atctatcaaa tgattgtctc tcgtttttta
    20041 ggactgtttg ctcaacccca taaagtcagc caaacaaaag ttacggttga atttggtaaa
    20101 gaacgattca tttttcggca aaatcgtgtc gtccaagcag gatggaaagg cgaaactgaa
    20161 actgagacgg aaacagtcaa atgggaaaaa ggaatgagaa tcaatcccga tttcgccatc
    20221 aaaaaagaac tatctgcacc gcctaaacca ttgtcagaag caagcttgct tggtcaaatg
    20281 gaaaaataca gtctgggaac cccagctacg cgtgcagaga ttatcgagaa actaatcaaa
    20341 tctgaactga tggaacgtac ccctcaagga ctgcaagtat ctcctaaagg taagcaatta
    20401 ttagtattgg taaatccctc tttagtaaca ccagatttga cagaaagctg ggaaaaagac
    20461 cttgaagcta ttgctgcagg aaagaaacaa gcaggaacct tcttgaatgg aatcgaaaaa
    20521 gaaacaaaac gcttggtgaa tgagatcaaa tcaagcaaac aagaatatca agatttctcg
    20581 attacccaaa agaaatgccc agaatgtggg gccaatctgc gcgagaaaaa cacacgtgac
    20641 ggcaaaatct atgtctgcac caattcagat tgcaactacc gcagaagaaa agatccaaaa
    20701 gtctctaatc atcgatgccc gcaatgtcac cggaaaatgg aaatcatcga aggtaaaaac
    20761 ggtgcttatt tccgttgtaa atacgatgga acgacagaaa aattgcttga taaaaaagag
    20821 cagaagaaga aaatgaccaa acatgaagaa cgacgcttgc tgaaaaaata tgcacaagca
    20881 gaagaaccag aagaaagccc attagcagca gcactaaaag cagcaatgaa gaagtaataa
    20941 gatatgaaag aggttgtgat agaagtggtc agcttcaaga aataagaagg aatttacgaa
    21001 aattgcttct caaatttttg tgaaatttcg gcttatttcc gaagaagcta cttctggaac
    21061 accatttatt cggaaaaaag gactgtgaca agacttttgt cacagtcctt tttcatttaa
    21121 aaaaatgttt acttcatttg acgtatatta ttggtgaggc ttcaattcat ttggcttcac
    21181 tttttctgtt tctcttatga tataaacagg acgctgtttc gtttctaaga agattttccc
    21241 aatatatttc ccgatgattc caagacataa caattggatt ccaccaatga acaggaagat
    21301 cgagaccatg gaaggccatc ctgatgtagg atcaccaaac aacaatgccc gcaaaataat
    21361 gaacaaaatt gcaataccag aaccgacaca agacaaagcg ccgataaatg aagcaatcgt
    21421 taacggtgca tccgagaagt tcacgatacc atcaatcgag tagttgaata atttccagaa
    21481 ggaccaagat gtttcgccag ccacacgttc gcggttttca taagaaatat attctgtttt
    21541 aaatccgacc cagctaaata atccttttga aaatcggttg tattctgtta attctaggac
    21601 ggcatccacc atttgacgag tcatcaagcg gaaatcacga gcgccatcga ccatttctgt
    21661 atcgctgatt ttattgatca actggtagaa ttttttcgca aagaaactgc gtatcgcagg
    21721 ctcgccttca cgagtcgaac gccgggtgcc gacacaatcg atatcagaag tagtgatccg
    21781 gttgatcatc tcaggaagca gttcgggcgg atcttgcaga tcaacatcca tcacggtcac
    21841 taaatctcct gtagaagctt gcaatccagc gtacagagcc gcttctttcc caaaatttct
    21901 ggaaaatgag acaaagcgga cataaggata agctgaggct agcttcttga ttgctgctaa
    21961 cgtaccgtct tttgatccgt cgtcaataaa caaatattcg aactcatggg cacctttgaa
    22021 tttttcaacc tcatcaaaaa aaagaggaac agttttttct tcattaaaac atgggacaac
    22081 aattgatact ttcatcgttt ctatcctttc aatcgtatgt ttatatatgg tttacattca
    22141 aaatttaata aaccttaaat aacaccttta aaatttgatt ttacgtaaaa gaacaggtat
    22201 tctttttgta aactaacgta tttagtttac catgtttcct cggtttatca aacttttaag
    22261 aacaattttt attagttggc aagtagtttt ggatagagaa aaaggaggaa gttccagtct
    22321 attgactagg agcaaacaaa atgaaaatac acacacctat cggcttttta tggttagatg
    22381 cagatgaatc cggattgact gctgtatctt ttcagccaat ttcagaaagc gaatcagcga
    22441 atcagcgaat tttaacgcgc acacaagaac agtttgaaga atattttgcg ggaaaaagaa
    22501 aagaatttga tgttccttta tctataaaga gaggtacgcc ttttcagcaa aatgtctgga
    22561 aagctttgtc gaccattcct tatggagaaa tccgaacgta taaagaagta gccgttgctg
    22621 ttgatagtcc gaaagctgtg cgagcgattg ggcaggcaaa tcgaaccaat ccattgccaa
    22681 tcgttattcc ttgtcatcga gtgatcgggc aaaatggaaa actgacagga tatatgggaa
    22741 acgcaataga tggggttgaa atcaaacgcc aatt
//
LOCUS       DAYYCW010000045        22633 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000045 DAYYCW010000000
VERSION     DAYYCW010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.045"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(227..1114)
                     /locus_tag="ACHAYA_001521"
     CDS             complement(227..1114)
                     /locus_tag="ACHAYA_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8605322.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="ACHAYA_001522"
     CDS             complement(1111..1980)
                     /locus_tag="ACHAYA_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8605323.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="ACHAYA_001523"
     CDS             complement(2117..3403)
                     /locus_tag="ACHAYA_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8605324.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="ACHAYA_001524"
     CDS             3676..4254
                     /locus_tag="ACHAYA_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8605325.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="ACHAYA_001525"
     CDS             4254..5183
                     /locus_tag="ACHAYA_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8605326.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="ACHAYA_001526"
     CDS             5180..6916
                     /locus_tag="ACHAYA_001526"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8605327.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACHAYA_001527"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACHAYA_001527"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM8605328.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="ACHAYA_001528"
     CDS             complement(7812..8321)
                     /locus_tag="ACHAYA_001528"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM8605329.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACHAYA_001529"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACHAYA_001529"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM8605330.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9622..10713)
                     /locus_tag="ACHAYA_001530"
     CDS             complement(9622..10713)
                     /locus_tag="ACHAYA_001530"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM8605331.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACHAYA_001531"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACHAYA_001531"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM8605332.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACHAYA_001532"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACHAYA_001532"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM8605333.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACHAYA_001533"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACHAYA_001533"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM8605334.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14676..15650)
                     /locus_tag="ACHAYA_001534"
     CDS             complement(14676..15650)
                     /locus_tag="ACHAYA_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM8605335.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15631..17514)
                     /locus_tag="ACHAYA_001535"
     CDS             complement(15631..17514)
                     /locus_tag="ACHAYA_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM8605336.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17532..19757)
                     /locus_tag="ACHAYA_001536"
     CDS             complement(17532..19757)
                     /locus_tag="ACHAYA_001536"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8605337.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19872..20906
                     /locus_tag="ACHAYA_001537"
     CDS             19872..20906
                     /locus_tag="ACHAYA_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8605338.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20973..21986)
                     /locus_tag="ACHAYA_001538"
     CDS             complement(20973..21986)
                     /locus_tag="ACHAYA_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8605339.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22064..22252)
                     /locus_tag="ACHAYA_001539"
     CDS             complement(22064..22252)
                     /locus_tag="ACHAYA_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM8605340.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         6446 a         4863 c         4096 g         7228 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agttttctag atttcataaa aaatcgctcc aaactgatgt aagaattgta cctagtatag
    14461 acataaaaaa atcgactgtc aatcattttt atgaaacttt ttttaatttt ctgatagtta
    14521 actttgtttt tcagaaaaat aacaattggt tttattgaag aaaatagtca tgaacaaaag
    14581 tatctgagaa cttttttact ctcttgttca tgacatatag aataaagcag ctttgtcttg
    14641 taaactccgt ttttcaaata ataatctaaa gattgctact gaataataaa gccgatttca
    14701 tggtcctctt cgctgctgat tcggtaaggt gactcaacat ccgctcccca gctattgata
    14761 cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg ttcgtctttc tggcggtagt
    14821 tcttctatat gtgttgcctg ttccagttct tctgctttga atggtaaagc ggaaaaagca
    14881 aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt cttgatcttt atccgtatta
    14941 tccaacgttt gatttcttgt tagttctacc catttcgttt ccatatgcat gccgcattct
    15001 tggggaacaa ggtatgggga aacggggagt cctgccactt tataaatacc ttcgacacca
    15061 ccagccatcc gatccggata tgtttcccct gataggcctt catactcaaa aaattttgct
    15121 ttcgttggca taatgaacct taaacccaat gcgggtaatt ccggtaatcc tttttgtcca
    15181 taataatgca tattgacaag cattttaccc tctccagcga tttggtagga aattgtcact
    15241 tgggtttttg gtactgtgtt gatttccagt gtaaaaatga tttcaaccgt ttcagcgtat
    15301 tcattacttg aatattgatt atttactgga gcaatcggca aatcgatctt tttattgtcc
    15361 accagtactt ctatttcttt gacttttgga tacaatccgg ctcctaacca cataccggat
    15421 ttaaatccaa atccgctacc tctgtcatta tcggtcgttg ctcgccaaaa aactggaagt
    15481 gtttcccggt atagccattc ttttccgttt ttattgagcg attccagtcc gcctcttgta
    15541 taagaaaaaa tataatgcgt attccccgat ttgacaccta atgtacagtc gccgaagacg
    15601 attgcaactt tttccgtatc catatgactt ttatttactg tatttttcat aataatatct
    15661 tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa acgatcccat tccctgagaa
    15721 ttcatagtca cttggtttat cgtcaaagtc accgccatag cgcatcactt ctctgcctgt
    15781 gattggatca gtcacttgga tcgcctgatc tttaaaatcc catatatagc ctccttggta
    15841 catttcataa cgatcaagca acgtcatgta agaatccatt ccacctaacg aattccccat
    15901 gtcatgcata tattcacata agatgaacgg tttttccgct ttattttcaa ggtagtcaat
    15961 gatctcttct ggtggcgcat acatgcagct ttttacatct gaaatcgaat cttcgaatgc
    16021 tggatttgga aaattccctt cataatggac aagtcgatcg gggtctactt ttttgaagta
    16081 atcgttcatt tgtcgaatgt tttcttccgc ataagattca tttccaagag accaaaaaag
    16141 aatacttgga tggtttttta atgtttcgaa attcgttttt gctcgatcaa gaacagccgc
    16201 ctgccactga ggtactgcac ctggtacgtt gtaagaaggc tcaaatgcgc ccattttttg
    16261 ccatgagcca tgggattcga ggttcgtttc cgccatcaca tggattcctg cttggtcgca
    16321 taaataatac caagtgatct ggttggggta atgagatgtc cgtacgccgt tgatctttgc
    16381 attttttatg atagccaaat ctttttccat atcttctata gtgatggcgc gtcctttttt
    16441 cggatgccac tcatgtcgat ttaccccgtt gataatcatc cgttttccat tgaagtagat
    16501 gattttatct ttgatttcga ttttacgaaa accgaaggaa tagtcaatat actccgttac
    16561 tcttttttct tcgtctatcg tctccaattc aagcttatat aaattgggct gaaaatgccc
    16621 ccacagtgcc acattttcaa atccatcgat gcgaatcgtc atttgctctt ttaacggata
    16681 ttgctgttga tagatttcct tcccatcagg atctagtatc gctacattca gctgactgtt
    16741 tttggctact tgctcaaaag acaaatctac ttcaagtttg ccattttgac atttgtcatc
    16801 taagcgaggt ttgatccaaa gatcttctaa atggttttgt cctcgagcaa ctaagaagac
    16861 atctctgaag atcccaaaaa atcggaaaaa gtcctgatct tccaagaatg cagctgtact
    16921 tcttttgtaa acctccacag ctagaatatt ttcttcttcc aaataaggtg ttaaatcaaa
    16981 ttcagacgga gtgaacgaat cttcggcata cccgataaaa tgaccattca accaaataaa
    17041 cattgcttgt tctacaccag caaaatgaat cgagatttgt tttcccttta gaccttcttc
    17101 taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct ttagcagctg aaaagatccc
    17161 ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg cgaaatacat gtccctccca
    17221 cggatacatc gtattgatgt aatggatctt gtcataattt gctaattcaa tatgactagg
    17281 aacagtgatt tcatcaaaag aacaggcatc aaaatctttt ttataaaatg tttttggtcg
    17341 taccgaagga tttggcgaat aacaaaaacg ccatgatcca tttaaagact gtttaaggga
    17401 ggatgtggtt tctacttctt cccttgttgc ataacaaaca tgatcgctat gtgcatttga
    17461 ttggttcact ctaaaaactt gtggatcatc taaccatttt atatttgctt tcatactttt
    17521 ttccttctct ctcataactt tttgaaatga agcagttctg tttggaaatc accttgaaaa
    17581 tcggggaagt aaagtccaac tgtcatcaat tcggcccccg aaaatatccg cttcgaggtc
    17641 tgttcctgat aaagtgcctc atcatctaac cccatcagat atacttcatg gaacgctggt
    17701 tgagctgaag ctagtatcct ccctaaaaac acaatagctt catcttgttt aggagacaca
    17761 aacatccatg cggcaatatt ttcttcaaaa ggactcgcca aacgataata atggccatac
    17821 tggatcaccg gacgaatcgt ttgataggat ataatttggt cttttacttg ctttttttct
    17881 tcaggagaca gctttgtcaa atccagttca taaccaaaaa cagcgctcat agctgtttct
    17941 cctcttgttt tcaacgaagt agatctgtcg gtctgatgat ttggtacagc tgatacatga
    18001 gctgtgatgg acgaaatcgg ataagcaagg cttgttccat attggatttt catacgctcc
    18061 actgcatccg tgttatcact tgtccatgac tgcggcatat aataaataaa cccggcatca
    18121 aaccgtccac ctccaccaga acagccttcc cataagattt ccggataatc agttgtcaaa
    18181 tgttcaagta gttcataaag cccgagcata taacgatgcg ctacttctcc ttgtctctgt
    18241 gggtcaaggg taatcgaata gacatctgaa agcgaacggt tcatatccca tttgatgtag
    18301 tcaagaggaa tcgtatctaa aatctttctc atttgtttct caatcgtctg ccgaacatct
    18361 aaccttgtaa aatccagtac atgttgtgat ctggaagcag aaggcattct acctggctcc
    18421 tgcataagaa aatcaggatg tgttcgatac agttcagaat caaccgagat catttcaggt
    18481 tcaaaccata aaccaaattt caatcctttg ctatgagcat aatctgctat ttcacgtaat
    18541 ccatttgtca acttgccttc atactcgaac cagtctccta atgaagaatt gtccgcatct
    18601 ctttttccaa accagccatc atctaatacg aacatttcga tgcctagctc ctttgcttca
    18661 tcaatgatcg ccttgatttt ttcactattg aagtcaaagt aagtggcttc ccagttattg
    18721 accagaatcg gccgttcagc aaattgatga cgacctcttg cgattctttc acgtagcaac
    18781 tcatggtgga tctgactcat tttgttcaat ccctgacttg agtaagaaag gatcatttca
    18841 ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa aacgatggct gttgatccca
    18901 ccgacgactc tcaattgatt gatctgatct ttttcaagtt cgaaagaatg gttgccggaa
    18961 taaacaaatt gaagtccgat cgcttctcca gtattttctg tcgtatgatg atgaactaaa
    19021 gcaataaagt tattcatatg gtgactgctc gttcctcgac ggctttcaaa tacttttgtt
    19081 ccagataaaa cagattgccg tgaaatctgt ctttcattga catgtgctcc tggcaaagca
    19141 atgacttcat caaatcgcct agtatgggca aagtctagct ggaaactagc cgctttttct
    19201 agtttgattg tttctccggt ttcatttctt atctttacag atcgtgtgat cactgctcta
    19261 tcagtaaata tggtatagaa caaatcgaaa taaatcgctg tttctctatc cttaagtgaa
    19321 atcaccaacg tttgtgcctc tgacttgtcc atcacataag tttgaggcaa gccttcgatc
    19381 tctggcttcc catctgtaat ataatggctg tcatatctca gatccagtaa gtctgatcca
    19441 ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca tgctgccatg agaagagtat
    19501 tcttgcggta gtgtatcttt cgacagagac cggtcaggat ttaagggaac atttcctgaa
    19561 aatccacgat ctcttcgggg ataattttta ttgtctttgt actgcttgac tggtttgcca
    19621 aaataaatat gcgccaatac attgctttct tctatcttta tcagataaga aagaaatgta
    19681 tttcgtaaat ggaagactcg gctcttttca tcaaataaga tgaaattttt atctgttttt
    19741 ctatgcgtaa tatccatcat ttcactccca acaaaacgga aaccgtttac ttttttttat
    19801 aattcctgta tactgtttac taaacaattt tgtcaataaa aattttttat ttttttgagg
    19861 agtgtgtaaa aatggttgga attagagatg tcgcacgaaa ggcaaatgtc tctcctgcta
    19921 cagtttcccg tgtgttgaat aacgatcaga cgatccatgt ggctcagact actcggcaac
    19981 ggatagtaga ggcagctgct gcgttggatt atgacatcag tactcgtaaa tatatcaaaa
    20041 agagaatgcc ttcgattggt gtgatctcta cgatcggcaa agcgagcgaa gaacaggata
    20101 tctattacaa acatttgcga gtagggatgg aagaagaagc tcgacgtctg catttaggga
    20161 tgaatcgaat ctataatcta tctgataatc ctaaacaatg ggaagatcta gatcagttag
    20221 gtgcggtgat cgttgttggg actgttacga aagaatcgat taccgccttg ttaaaacaaa
    20281 acatgcaaat agtcgtcgtg gataaccctg acatccagca agaggtcgat atggtgtatg
    20341 cagatcttga acggatgact aaaaccgtac tggaattgtt cttagaaaat ggtcataaaa
    20401 gaatagccta tatcggggga tataaagtcg atatggatga attcggtggg aagcagattt
    20461 ctataaatga aaagaggatg cgtgcctatc agcattttat gatcgatcat cacttagaaa
    20521 aatatatgtc ttatcatcta ggtggctgga cagaaaaaca aggtgaggta cttgcgacta
    20581 atctattaga aaatgaatcc cctcttccta ctgccattct tgtcgggagc gatccaatgg
    20641 caattggtgt ctatcgggca ttgaaacaaa aaaatatcca aattggagaa gagattgttg
    20701 tagcaagctt tgataatatc gagacaatcg caaacctctc ccctggcttg accagtgttc
    20761 aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat ggcattggag cgaattgacg
    20821 gtatacggga cgaaccaatc attttgactt atcctgcacg attgattgtt agggaaagtt
    20881 ttatcccaag aacaaaaagg tattgaaaga gaggttgtga aaagccgctt cgtttcttca
    20941 tactcaaaac gaaacggctt tttcacaacc tcttacttat acttattcac aaaatccaat
    21001 gtctgctggt aacaattttg cgattctttt agcgtgtaat caaattggta ttcatgggta
    21061 atctcttttt ttgtatctgt ataaaacaag gattgcacgg gtacctttaa ttctgtcaac
    21121 cgattttcta atgcttgacc ttgctcctgg aatgagtatg catttccgtc agttatatac
    21181 gttggcggaa attccttaga aacatggtca gccacactag cttctttcaa ttgctcactc
    21241 gttttccaat cttttgttcc aatcaacgac cacgcaacgg ttttgacgaa aaacttcata
    21301 aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat caacaggtcc gcaataagaa
    21361 atggttcctt tcagactatc ttttggaagt agctgtttga ttttcatttg tttagcatac
    21421 gcatcattcg tttgtatagc tgcatattgc aatgcgattt gtgaacctgc actatctcca
    21481 ccaaaaaata attgttccat gtctagcaat ttgtcgtctt tttcttcagc taataaatct
    21541 ttgatcagtt cattcacttg aagaacttga ttagggtatt ctgaatcagg cgctaattca
    21601 taattcatcg caaccactgc gatgttggcg tctgaaacta attttgtcgc aaattctttg
    21661 acacccgatt tatctccgcc gacgaatcct ccaccatgta cccaaaatag aacaggaaca
    21721 ggccccttgc tgttttttgg atagtagatg tcataagtat tctctttata cttggactca
    21781 tacgtcaaat ctgttttttt cgccacgttt ttttctgctt gatcgaacgt ttttttatca
    21841 gtgatttgaa cagtatttga aaacatatgg ttgatcaaga atgctccagg tctgggggaa
    21901 agctggaagg caagaaagat tgcaaacgcg atcccaagaa tgattccgaa gaaccatccg
    21961 ataaatttaa gaattttctt tttcataagg ctccctcagt agttagtatt aagcattctg
    22021 attataccat agtgttattt cctatcaagc tctcctagat gatttaatga ttttccccaa
    22081 acttcttctg caatgctttt gacaaaagaa attttttcca ttgttcttca aacaaaacat
    22141 tttcttcttc tgttgaagca aatccacatt gaggacttaa gcacaaacga tccaaaggca
    22201 gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg gtgctcttcc atttcgccat
    22261 ttttcgttac agtcaaaatc taagacaact aatttatctt cagagacttt ctctaaaaga
    22321 acaaaactcc cagcacgttc actatcgtat tctaaataat aggcacggac attttcttga
    22381 gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg ccgccaaaat cgaataataa
    22441 tttccgcgtc aaacatgtat atttagtgtt gaatcatttg agaatatgaa aataaaaatc
    22501 tttttgatca atcagttaat caaattgaaa taacgaaaaa tctgagacag aatgacgttc
    22561 attctgaccc agatttgatt ttgcacaact caacagtcct tattggcaaa tcgccaaaat
    22621 aatgtagact aac
//
LOCUS       DAYYCW010000046        22180 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000046 DAYYCW010000000
VERSION     DAYYCW010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.046"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            245..2080
                     /gene="tdc"
                     /locus_tag="ACHAYA_001540"
     CDS             245..2080
                     /gene="tdc"
                     /locus_tag="ACHAYA_001540"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8605341.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2385..3863
                     /locus_tag="ACHAYA_001541"
     CDS             2385..3863
                     /locus_tag="ACHAYA_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8605342.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3829..5160
                     /locus_tag="ACHAYA_001542"
     CDS             3829..5160
                     /locus_tag="ACHAYA_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8605343.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5274..7826
                     /locus_tag="ACHAYA_001543"
     CDS             5274..7826
                     /locus_tag="ACHAYA_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM8605344.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7833..9272)
                     /locus_tag="ACHAYA_001544"
     CDS             complement(7833..9272)
                     /locus_tag="ACHAYA_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8605345.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9247..9429
                     /locus_tag="ACHAYA_001545"
     CDS             9247..9429
                     /locus_tag="ACHAYA_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605346.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9618..9980
                     /locus_tag="ACHAYA_001546"
     CDS             9618..9980
                     /locus_tag="ACHAYA_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM8605347.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10167..11024)
                     /locus_tag="ACHAYA_001547"
     CDS             complement(10167..11024)
                     /locus_tag="ACHAYA_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8605348.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11144..12055
                     /locus_tag="ACHAYA_001548"
     CDS             11144..12055
                     /locus_tag="ACHAYA_001548"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8605349.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12062..13713
                     /gene="mdcA"
                     /locus_tag="ACHAYA_001549"
                     /pseudo
     CDS             12062..13713
                     /gene="mdcA"
                     /locus_tag="ACHAYA_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13815..14114
                     /locus_tag="ACHAYA_001550"
     CDS             13815..14114
                     /locus_tag="ACHAYA_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM8605350.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14104..15780
                     /gene="mdcD"
                     /locus_tag="ACHAYA_001551"
     CDS             14104..15780
                     /gene="mdcD"
                     /locus_tag="ACHAYA_001551"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM8605351.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15784..16377
                     /locus_tag="ACHAYA_001552"
     CDS             15784..16377
                     /locus_tag="ACHAYA_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM8605352.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16401..17477
                     /locus_tag="ACHAYA_001553"
     CDS             16401..17477
                     /locus_tag="ACHAYA_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8605353.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17502..18371
                     /gene="mdcB"
                     /locus_tag="ACHAYA_001554"
     CDS             17502..18371
                     /gene="mdcB"
                     /locus_tag="ACHAYA_001554"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM8605354.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18482..19246
                     /locus_tag="ACHAYA_001555"
     CDS             18482..19246
                     /locus_tag="ACHAYA_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM8605355.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19735..21828
                     /locus_tag="ACHAYA_001556"
     CDS             19735..21828
                     /locus_tag="ACHAYA_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8605356.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21853..22155
                     /locus_tag="ACHAYA_001557"
     CDS             21853..22155
                     /locus_tag="ACHAYA_001557"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8605357.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7180 a         3788 c         4725 g         6487 t
ORIGIN      
        1 ttatttgttt gttcgctttt tttaggttat ctattttaga agactgattt attgaaaaat
       61 tgttcgcttt tttgcgttga attatcagaa aaatccgact ttatttattt ttgtttgtaa
      121 aaatctacgt ggatttgatg ttataaaaaa aaaattctca tttttggcgc tatagtgaag
      181 aggtgttaca gataaacaca tgtatctgca agacattgac caacaaaata tggagggaag
      241 aaaaatgaaa gatatggata tcaaggccgt ctttataggc gacaaagctg aaaatggacc
      301 agtttataaa atgttgttga acaagatggt agatgaacat cttggatgga gagaaaatta
      361 tattccttca gatatgcctg caattagcga aggagataag ctcactccag attatctagc
      421 cacccgcgac catatgatag aggtcttgga tgaagtcagt cagcgattac gcgcaggatc
      481 gatcccatgg cattcagcag gccgttactg gggacaaatg aatgctgaaa cattgatgcc
      541 tgctttatta gcttacaact atgcgatgct gtggaaccca aataatgttg cattggaatc
      601 ttctatggca acttcgcaaa tggaagcaga agtaggacag gattttgctt cattattcaa
      661 tatgacagac ggatggggac acattgcagc agatggttct attgctaatc ttgaaggatt
      721 atggtacgcc cgttgcatca aatcgattcc tttagctgtc aaggaagtgc tgcctgaaaa
      781 agtgaaaaaa atgtctgagt gggaactttt gaatttatct gttgaagaaa ttttggaaat
      841 gaccgaaagc tttacggacg aagaaatgga tgaggtcaaa gcagcttctt cccgtagcgg
      901 aaaaaatatc caaagattag ggaaatggct ggtccctcaa acaaaacact attcttggat
      961 gaaggcgtta gatatttgcg gagtcggctt agatcagatg gttgctatcc cagttcaaga
     1021 ggattatcga atggatatta atgctttgga aaaaacaatt cgtgaattag ctggacaaaa
     1081 aatcccaatt ctaggtgtgg ttgctgtcgt aggaacgaca gaagaaggtc aagtagacag
     1141 tgtagataaa atcgtccaat taagagaaag gttaaaagat gaagggatct atttctattt
     1201 acatgttgat gccgcatatg gcggctatgc gcgttcactg tttctaaacg aagcaggaga
     1261 atttgtgccg tatgcttcct tagctgaatt ctttgaagaa catcatgttt tccaccactg
     1321 cgtaacgatt gacaaagagg tatatgaagg gttcagagct atttcagaag cggattctgt
     1381 cacgatagat cctcataaga tgggctatgt cccttatgct gctggaggaa tcgtaatcaa
     1441 acataaaaat atgagaaata tcatttctta tttcgcgcca tatgtgtttg aaaaatcagt
     1501 aaaggctcca gatatgttag gagcttatat tttggaagga tcaaaagctg gagcaaccgc
     1561 cgccgcagtc tggacagcac atcgtgtatt gccattgaat gtgaccggat atggtcaatt
     1621 gattggggct tcaatcgagg cagctcaaag attcagagaa tttctcgatc atctgacttt
     1681 tactgttaaa ggaaaaacaa tcgaagtcta tccattgaat catcctgatt ttaatatggt
     1741 caattgggta ttcaaagaac aaggctgtac agatttgaac gctatcaatg aattaaatga
     1801 aaaaatgttc gatcggtctt cttatatgga tggcgatgtt tatggtgaac ggtttattac
     1861 ttctcatact acatttacac aagaagatta cggtgattct ccgattcgtt ttgttgaaag
     1921 aatgggtcta acgaaagaag aatggaaaaa agaacagaaa atcactctgc tacgtgcggc
     1981 tatcatgact ccttatttga atgatgatcg aattttcaat ttttatacaa aaaaaatcgc
     2041 caaagcaatg gaaaagaaac taaacgaaat catccaatag aactgatttt tgtgtgaaag
     2101 aataacaaga gacatatgga gaacagacaa ttatttgctt catcaaaaca attgtatttc
     2161 cccctgtttt tcatatattc ctcgccaaga tattcttttt tataaaacgt tctataagtt
     2221 ggatcgttcg tttcatgtat caaacccagt tcactaagat cgttcaatgg atgtttcggt
     2281 gatactgggt tttgtactga atcgatgaaa ccttgtcatt tctgttcttg aacaaaaata
     2341 aaagaaaaca aaaacatatg tcgctattga aagaaggaat aaagatgaaa gatactggtt
     2401 catcaaagca aatcacgacc atgaccttta tcggaatgac ctgcgccctt gtagcaagtg
     2461 tacgaaatat tccagatgta gcagcaacga gctggacgat gattttttac atgctggttg
     2521 cagtattatt gtacgctttt ccaatctcat tgatctctgg tgaatttgct ggaatgtttc
     2581 cccaaaaagg tggtccagaa ctttgggtat cgaatgctct aggaaaaaaa tgggggttcg
     2641 ttgtctcatg gctgttatgg gtccaaatgt ttcctgggat ggtgatggtc gcttcagcac
     2701 tcgctccgtt gtttggtaat attatcgata atgtgccgct tggtttgaac agcaaattta
     2761 cattagttgt cattttagtc gtttattgga tcatcacttt tttgaatttg aaatttgata
     2821 tggcaaaaat cggcggaaaa gtcggtgttt ggttaggatt gtacgtgcct ttgacgatga
     2881 tgctcttatt agggtttgct gcgtggatca aagttgggat cgtaccaacc ggaacattag
     2941 gcagtttttc atgggacaaa ttaataccag atactacgac tgcatcctca tttgtttatt
     3001 ttgcgccgat catgtttatt tttactggta tcgaaatgtc ctctgtgtat atcactcgat
     3061 tggaaaatcc agttaaaacg tatattcgcg gagtatttgc cgcgttaatt tttatatttt
     3121 tcgtcaatat tttgaatgct ttagttgtag caaatgtcgt accaaaagga caaatggaat
     3181 tgaataatat cgcgcaatct atttcgattt attgccaaat tcttggtttg ccacatctta
     3241 ttgtgaatct gtttagttta cttgtcttca ttggtgtagc agtccaatta tctgcttggg
     3301 cctcaggacc agcaaagaca gtgacagaaa gtgctagaaa aggtgcgtat ccaccaaaat
     3361 tcaatttttg gaagacgaat caatttgacg tatccaaatc cgttattttg actcagtctg
     3421 tcattatctc aatctttgca cttttttatt tgctgattcc tggcgtcaat caagcatttt
     3481 tgatgctcgt aaattcaact actgttatct actgtatcgt ctatgtcatc atggggatcg
     3541 cagttttgag attgcgttat acacatgcaa agttgaatcg accatttaga attggaaaaa
     3601 aagagaaaaa gagcaatctt ggagtttgga ctgtcgttat tgttttattt gcggcaatcg
     3661 gattttctgt cggactgacg atgaaagctg gaacatggat caatctaatt gcagtcacag
     3721 ccatcagtgt cattttgttt gtagtaccac tctgtattga aaaaataaag aaaccttctt
     3781 gggaacagga agtcgccatg ctgcttgaaa aagaaaaagg tgaacttgat ggaaaaaccg
     3841 gcaatgaaac ggactctaac taatcgaaat atccagttaa tcgcattagg tggcgcaatt
     3901 ggaacaggat tatttttagg ttcagcaaag gcaattgcat cagcagggcc agctgttatt
     3961 ctcgtttatt tgatatgcgg tttggctatt tatggtttga tgcgggccat gggtgattta
     4021 tttttagcaa atagtacatt taactctatc agtgatttcg ttgactatta tttaggcaaa
     4081 aagtgggcat tttttgtcaa ttggacttat tggctttgct ggcttggagc tggacttgcg
     4141 gaattaacag caataggatt gtatatccat ttctggtttg tacatgtacc agctttgata
     4201 agcggaggta gcgcactaat gatcttaatg gcagtcaacc ttgttgcagt caagtggttt
     4261 ggtgaattgg aatctttttt ctcaatcatc aagatcttgg ggattttcct ttttatctta
     4321 ttgggtatag gattgagtgt ctcagatttt catacccagt ctatcggaat caaagaaaat
     4381 tttggacaga tcgatgaatt actaccgttt ggctttgctg gaatggtagt ctcatttcct
     4441 atggttttat tcagttttgc tggaattgaa atgatcggct taaccgttgg cgagacaagt
     4501 gatccgcaaa agaatttgaa aaaagcaatc aactgtctgc cttggcgtat tctctttttc
     4561 tatataggaa cgattttgtc gattttattt gttgttccat ggcattgttt agatttaaaa
     4621 gaaagtcctt tagtgactat gctgcaagtg atccattgcc aaggcggagc agcaattgtc
     4681 aatctaatga ttttggttgc ttctttgtcc tcagcaaata gtgcagtcta cagtactagc
     4741 aggattttat atcaaacagc aaaagaaaaa agtttgcccc attggttttg tgcgctttct
     4801 aagaaaggcg tacctttaca cggtatttta attacaggaa tgttattttt tgccggtctt
     4861 tgtctacatt tttttgtagc tgatagtcga ttgttttttc agcttttggc aggcatgaca
     4921 acgagttgtt ttctatttgt gtggtcttcg atcttgtgtg cacatgttca atttattaaa
     4981 gaaaagaaac ggaacactac acaaagatat aaagatcttg ctttgttggt gttttttact
     5041 tcaattgctt tcagcttgtt attcgaaagc acgacacgct ttattccttt tttattggtt
     5101 atctggtttg tattcttaaa ttttgtatat aaaagaataa gcaatagaaa tatcagttag
     5161 gcccagatac attttgcaaa acgaattatt tcagaccccc ctgaaataat cgatacgcct
     5221 gaaaaaactt ccctgcgtat aataacagta tcaattaaac aggaggggat aggatgaact
     5281 ggtacagatt gacggtagaa gatgtattga aaaactatca aacatcaaca gaaggcttgt
     5341 cgcaagaaga acgacagaaa cgcctgactg atcaaggata taatgaaata aaagcaaggc
     5401 agcagacaaa acgatggaaa aaaatagcaa agcattttac tgatctgctg atgatcgtgt
     5461 tgatcattgc ctcttttttg aaatttgctt ctagtgaata tattgaagga agtattattt
     5521 tattcgtagt gatcgtcaat ggactcgtta gttactggca agaaagaaaa gcggaagaat
     5581 cattgaatgg tttgaagcag ctgatgggac aagaagcgat agtactatca aacgggatgc
     5641 aagaaaaaat ccctgcgaaa gaactggtaa aaggagatgt cattgtctta cgtgccggag
     5701 atgtcgttcc agcagatatc cgattaatcg aagcacatga tttattagtg gaagaatcga
     5761 ttttgacggg agaatcagaa gcatctgaaa aaagtcatgc cgttttgaca gaagaagaat
     5821 cgatcggcga ccagaaaaat atgggttttt caggaacgct tgtacaatct ggttctgcag
     5881 tcggtgtcgt tgtagaaaca ggaaacacga cagaaatcgg taaaatcaat caagcattgc
     5941 aatcggtcga acaacaaacg acgcctctga ttaaaaaaat caaccgatta aacaaacaaa
     6001 tttttcaagg cattctttgt ttgagcctat tcttagttat ttttacgaca tttcgatatg
     6061 gtatggattg gcatatcctt ctttcggcta cgattgcttt ggtggtgtca atggtacccg
     6121 aaggattgcc agctgttttg accatgattc tatccgttgg agtacatgaa atggcaaaag
     6181 aaaaggcgat catcaaagga cttccttcgg tagaaacatt aggttctatg acagtgatct
     6241 gttcagataa gacaggaaca ttgacaaaaa atgaaatgac agttgtggat gtagctgcaa
     6301 aagaagaagc ctgcgtgttg tccattatga aaaactgtca ggaattaaaa acaaaagaag
     6361 aacagaaaac agaaaattta agtggcaatc cgacagaagt tgcgctgatt cgacacacag
     6421 aaacagccaa tctacctttg aaaaaaatag aagcaaagat tccgttcagt tctgaataca
     6481 aatatatggc aacgatgcat gcggaggaaa aaggagcggt gatttacgtc aaaggtgcac
     6541 cagaagtatt atttgctaaa tcaactcttt cttctgcaga acaagaagca tggagtcaga
     6601 cagctgcaga atttgcgaga aaaggtcagc gtgttttagg atttgcttat aaaaaagtag
     6661 attcgaaaca ggaactcacg catgagactt taacccaatt aacttttgca gggatcgccg
     6721 gattgatcga tccgccaaaa gaaagtgcag tcaaagctgt aaaggaatgc caacaagcag
     6781 ggatctcagt gaaaatgatt acaggggatc acaaagacac agcaaaagcg attgcggaac
     6841 aaattggctt aaaacatact gccaaagttt tagaagggat cgatttggat cttatgtctg
     6901 atgaagagtt gattcagcaa gtgccaatag tcgatgtctt tgctcgaacg acaccagaac
     6961 ataaactgcg aattgttaaa gctttgcaga aaaatggaga aatcgtcgga atgaccggag
     7021 acggcgtaaa tgatgcacct gctttgaaaa gatccgatgt aggaattgct atgggaatca
     7081 aaggtagtga agtaagcaaa caggcagctg atatggtttt aggagatgat aatttccata
     7141 cgattgcaaa agccgtaaaa gagggccggc ggattttgga taatttgcaa aaaaccatca
     7201 atttcttctt gccaactgca ttagctcaag gattgatctt gatttgggca cttatgctga
     7261 atcgcccgct acctttgtcc ccagtacaga ttttatgggt caatatggta acgacaatca
     7321 ctttatctta tgcactaggg ttcgagtcgc cagatccaga aatcatgaag cgcccaccaa
     7381 gagatccaaa gcaaggaatc ctatctggtt atcatttgtt tcggatcatc tatgtctccc
     7441 tactgatcat ggttcctgcc tatttattgg ctatgcagtt tgaagggcaa agcttacagc
     7501 aaaccattct tctgcaaaat atcgtcttag ctcaagctgt ttacatgatc aattgcagag
     7561 agttgtccaa gccgtcattg aaccaaggtc ttttgcaaaa caaagcgctt tttctttcat
     7621 taggtatcct tgctttattg caagggatgg ttatttttct accagtcggc cagcagctga
     7681 tcggtacagt aagtttaacg atgacacaac agttattgat tggagcaaat gtgattcttc
     7741 tattcttagt tgtagaaata gaaaaaggga tcatgaacaa actgtttcgc aaaaaagaaa
     7801 aaacagctag agaatacagt cgctagccgt tactataaaa aagttttgaa agtgagttca
     7861 gtcaaacgat tgagctcatt tttcgttgga atagaagaaa tgtatacaat agggatatct
     7921 agagaatcta gagggaaatt ggaaatcaag atatctgttt tttcaaaaga aatagtggaa
     7981 agatcggtta gttcgatagg taagaagcag acacgttttc ctaagtaatc ttttaattcc
     8041 tgctgcaaga acgccttcca tgaaggttct ccttggaaaa taaaatagac attgaacaaa
     8101 ttcggtttga tagaaaaaat cgtttgttgg atcagtagag acaagagttg gagaaagtaa
     8161 tttggttcat tgatttctat ttcttttttt agatcgttca gtaaattctc tttctgttga
     8221 tacaagtgtg catattgctg atcatatgtt tctaaaaaac gatggtattg acggtccaat
     8281 gcttcaatca atatcggcga ttcatgatat tttaatatga ggagagccaa gttatcagtc
     8341 agagatgttt tttttagttg atcaagagac agctctgaag ctagttcctc tacaaacgtt
     8401 tcgaggacct ctaaaaatgg agaatgatgc aggcagcgag cggtttcttg tccatagtta
     8461 ttgttgtaat tccaactttc cagaaagcag aaagaagcaa aagaaagctc agtgtcttta
     8521 agataaatac cttccgttaa atacaatttt ttcaatgcag agtcatacag ggaggagagt
     8581 gtgctgttgg tatggataga agaatctaat cggcaattcg cttttatacg gatcgtattg
     8641 ataaagaacc aacatgttcc gaaaatctct tctgtttcaa ccgctagttc agtttgactg
     8701 aggtttctta aaaattgaaa ataatgggat tcatggatcg aataatcctc gaaaaaatag
     8761 ttgttatgag tgaaaggaat cagcaatcga tgataaaaag tacgaatcgc acgctctttt
     8821 ccaagaatac aagctgtata tttcgtcaaa tcgatctgca agtcgtaact tgctaaacgc
     8881 tgattgattt tagtcacatg tcttttcaat gtcccataag aaagcccatg gtcggctaga
     8941 aaatcaggga cagcaacttt ttttgtaaaa agcagatttt tcagcagttc gacactgact
     9001 gattgtttga aaaaaatctc ccaaaggtca tttgttaata aattttgctg gttttgcaaa
     9061 ctgattcctg cttgatcgct gatgatgtcc cagccttcgg gcagctgaga gcgaatagat
     9121 tgaaggtcag aaaaaatcgt tcgttcggtt gtccctatga tttcagctaa ttcatttacg
     9181 gttatctttg tatgattcag caattgttct actaaagaaa catgacgtcg aatatctttt
     9241 tcggtaatga tttttttcat tagtgaatac atcaatcaga ctcccttcta ttctaagaat
     9301 acggagaaaa taaacggtgg tcaagtttca actgattttt caatcattgt ttatttttct
     9361 ggagcattta ctgatgataa gaatttcatc atgaacttga aaaatgatac cactaaaatc
     9421 aaaaaatagg tgggcaaaca ataataaaca ttttaatagt agatggtagc aagaccaaca
     9481 caggcggatt gaacatttaa gagttgataa aaaagaaaaa tatcgaagta tttgattagc
     9541 aactacttgt attcattttt ctattcttac gtggtatctt tgaataaagc gaatcaataa
     9601 gaaagagatg gaagcagatg ctaacgaaaa aagaactaat cgatcacatc aatgaaacat
     9661 acaatttgtc tccagaatat atttttaaaa aatttcctga ttattgcgta ttcagacatc
     9721 aaaaaagcaa aaaatggttt ggggtcctga tgtctgttcc agaagaaaaa atctatgatg
     9781 atgcaagtga ccataggatc atcaatattc ttgatttgaa ggtcgatcct gaacttggcg
     9841 gaattttgcg aggaaaagag gggtattttc cagcctatca catgaataaa gaacattgga
     9901 tctctattga tctacgtaaa atagaaaagc tagatgaact agcagatttg attgatggaa
     9961 gttttaaatc aacgcaatga atgtctagga caatagagaa gaggttgtga cagaaatgtt
    10021 tagcttcaag aaataagaag gaatttacga aaattgcttc tcaaattttt gggaaatttc
    10081 ggcttatttt ctaagaaatt acttctggaa caccgtttgt tcgaaaaaag agctgcgaca
    10141 agaattttgt cccagctctt ttttttttat tttcctaaat aaaattcccg catcgttttt
    10201 gcaatcattt tgatcaaagg atgatcttct gtgaagctag aagataaata aattgttcgt
    10261 gtcaagggca aatctttgat ttcccgcaag acgaaaggat catttttatc gtaagaccaa
    10321 gaaacttttg ggaaaaagct aacagctaat ccttgcttga caatagaacg taatgtcgca
    10381 ggatcatctg tcgtcgaact gatcgtcaat tcgatacttt tcgcttcaag tgctgcatcc
    10441 agtgtatgac ggaaggcgtt gctctttgac agcatcaaga tgggggtgcg ttggatatct
    10501 tctaattgca gatgttcttt tttagcaaga ggatgtttct taggaatggc gacataaaca
    10561 ggttctttca tgataggaat attggtcatg ccaggcttca tctccgaagt gatgtacagt
    10621 acaggatcgg taatatcatc tggtaaatga tgttgcatga tcgttaattg gatatcagga
    10681 tgcttttcca taaaaattcg gaccaagttc ggaatagaaa cagaggctac ctctaaatac
    10741 aatatgacgg aagtaacgat cttctcattg accgaagaca ataatttgat tccttcttct
    10801 aatgtgtcta atgtcttgac tgcatatgga taaaattctt ctgcataaac ggtcgtctca
    10861 atgtttctgc cgctatggta aaacaaaggg acacctaatt cgttttccaa atgtttgatc
    10921 gcacggctca aggcaggttg agaaataaat aattctttgg ctgcttgggt aacatttttt
    10981 gtttcgaata cttttttaaa atactgcaga tgaagcaatt tcataattat ccatcccatc
    11041 catacctttt tgttatggtt ttttcttttt ttggcatttt ttattatagc agaaattgac
    11101 taagatagca gtggaaaaag taagtgctga ggaggcgagg ctcatgagta caggtttctt
    11161 atttcctgga caaggcagtc agaagttagg gatgttagcg gatatccctg aaatgtacat
    11221 ggaacgttta gaaaatgtga cggggtatcg gttagttgat aaagaagaaa actatcaaga
    11281 tacggttttt atccagctag cattactgac aaaagcagta ttttatttag atgaaatgga
    11341 aaaaaacggt attcagcctg atattgcagc aggacattcg attggtgctt tttgtgcagc
    11401 tgttgcttgt cgatctttga gatttgagaa tgcagcggaa ctagtctatc accgcgcagt
    11461 cttaatgaaa gaagcttatc caagcggtta tgcaatgggc gtagtagtag gtgtgacgag
    11521 aagcgaagct gaggaaattg tagcaaaaac atttgatcct gaccatccag tttatctgtc
    11581 aaatgaaaat tgtccgatgc aacatacgtt atctgggagt atcgaaggtt tatccgttac
    11641 gttggcagaa gccaaacggc accaagcgca aacagcgaag ctgctgaaag tccctgtgcc
    11701 atctcactgt ctcttgatga atgatacggt caagaaattc aagccgtata tgcagtctgt
    11761 ccatctatct gatgctagat gtttgtatat aaaaaatacg gatggaagag ccactcagga
    11821 cgcagaagag attcgtaaag atttgttgga aaacctagct ttccctgtgc agtggaacca
    11881 aatgatggac ttggcaaaag aattaggaat ggatctgacg attgaatttc cccccggcaa
    11941 tactttgaca aagctgattc atacaaaatt tggtgtagat aaagggatcc gtacgatcaa
    12001 tctagaccaa catggtattg atgacgccac gtttctatat caaaaatgga gatgactaat
    12061 tatggaaaaa ccaaaccgca actgggcttc taaacgcgaa aaaaaagcgc agcgattgga
    12121 aaaaatcagc aacttgctca atggcaagta tgtagaaact gaaaaaatcg tagaagtaat
    12181 ggaaaaactg attgctcccg gcgacaaagt tgtattagaa ggcgacaatc aaaaacaagc
    12241 aagttttctt tcagaaagtc ttgaaaaagt agatgcagaa aaagtgaatg atctgcattt
    12301 gatcatgtct agtatctcac gaccagaaca tttgaatatt tttgaaaaag gaatcgcaag
    12361 caagatcgat ttctcttatg ctggtgcaca aagcttgcgt gtcgcccaaa tgattgaaga
    12421 cggcacaatg aaattaggtg atatccatac ttatttggaa ctttatggac gcttattcat
    12481 cgatttgatt ccaaatgttg ttttagtagc tgcagataaa gcagataaag acggcaactt
    12541 gtacacaggt ttcaatacgg aagaaacacc aacaatcatc gaagctgcag ctttcaaaga
    12601 tggtatcgtg atcgttcaag ttaacgaagt tgtcgattct ttaccacgtg tagatatccc
    12661 tggtgactgg gtagacgttg tcgtgaaagc agataaacct tatcaattag aagctttatt
    12721 cacacgagat cctcaaaaca tcacagaatt gcaaattcta atggcaatga tggcaatcaa
    12781 aggaatctac gcagaacacg gcgtacaatc tttgaatcac ggtattggat tcaatacagc
    12841 agctatcgaa ttgttactgc caacttatgg tgaatcacta gggttaaaag ggaaaatcgc
    12901 gaagaactgg gtattgaacc cacatccaac gatgattcca gccatcgaat caggttgggt
    12961 tgaaagcatc catagttttg gtggcgaagt tggtatggaa aaatatattg ctgctcgccc
    13021 agatgtcttt tttgtcggta aagacggcac gatgcgcagt aaccgaacat tatcacaagc
    13081 tgcaggacaa tatgccatcg atatgtttat cggttcaacc ttgcaattag attacgaagg
    13141 aaactcttct accgtaacaa aaggacgttt atctggtttt ggcggtgcgc ctaacatggg
    13201 acataatcct ggcggtcgtc gtcattctac accagcatgg caatcacttc gcgacaatga
    13261 tgatccatta gggaaaggcc aaaaattagt tgttcaaatg gttgaaacat atggttcaaa
    13321 taaaaaacca gtctttgtcg aagaattaga tgcactagct gttcaaaaac aagcaggctt
    13381 agccaatgca atgcaccagt gatgatttat agcgaagata ctacacatat cgtgacagaa
    13441 gaaggaattg cttatttgta caaagcaaaa acaaaagaag aacgacaagc agcaattgca
    13501 gcaattggcg gtgttacacc tcttggaatg actagcacga aagaatcact agatagcttg
    13561 cgtgcagccg gcatcgtgaa attaccagaa gatctaggca tcaaacgtag tgaagcgaaa
    13621 cgttcattat tagctgcaca aaccatcgat gatttgatcg aatggtcaga tggcctgtat
    13681 gaaccaccaa tgaaattcag aacttggtca taaaattggc gagaaaaacc ttttgtcgcc
    13741 atttacccag atggaagaag aaagacgaat aagcagaacg tgtatgctct gttttacaaa
    13801 aaggggagag atccatggaa caattagctt ataaatatcc aacgaaacag ccgatcaata
    13861 agaaaatcca tgtaggagtg gtcggttcag gagatttgga aatcttgatg tttccaacag
    13921 aaaaaacaga atctactgta aaaatttgta caggaagcga tggcttcggc gaagtatgga
    13981 acaatgtatt aacgagattt tttgatcgtt atccaatttc agcagatatc gtgatcaatg
    14041 acttcggtgc tacaccagga gttgtgtacc tacgattgac gcaagcaatg gaggaacttt
    14101 cagatgaaaa atagttttgt agaattaaac gccagagaac gagtgaacgc tctacttgac
    14161 aatggacgtg aattgattga tccattcgat caaatcatcg cgccaagttt accagcacaa
    14221 ggaatcgtcc ctgaaagtga tgacgggatc gttgtcagcc gtggcacgat caatggcaaa
    14281 caagcagtag taatcgctat ggaaggaaaa ttccaaggtg gcggtatcgg tgaagtttcc
    14341 ggtgcaaaaa tcgttgctgc acttgaacat gtgttagaag aaaataaaaa aggaaatgag
    14401 atctttccag tcattgtact agatacaggt ggtgttcgtt tgcaagaagc aaactacgga
    14461 ctgttatcta tttcagaaat cggaaatgta atcattgctc taaaacaata tgtaccagtc
    14521 atcggtttga ttccaggacg tgtaggatca tttggcggta tgtcgattac ttcagcatta
    14581 atgagttatt taatcgcaac aaaaaaagcg agagtcggtt tgaatggacc agaagttatt
    14641 gaacaagaag caggcgttcg agaatttgat tctagtgaca aagacttgat ttggaataca
    14701 ttaggatctc gtcaacgggt agaatcaaaa atcattgatg aattagtgac tgatagtgta
    14761 gaagcaatca aagctgctgt catcgcagca atggacgaac gaaaagacac gcatcgcagt
    14821 gaaagctcag cattttattt atcattactt gattctttag atctttcaaa accaatggcc
    14881 attgacgatt acaatgaagc aattgctaac catcaagcaa aatcaattga cttaccagaa
    14941 gtagcagaag gaaatgaacc agctgcagca agtgttggtt acgattggtt tgcggcacta
    15001 actggcatga aaaatccaac ttcttctatt agtactgtgt attatggtaa atcaagtaaa
    15061 ttcatagaag aagccatcgt gacatcaatc gtacctgatc ctaaaaaccc gatgtaccgt
    15121 gtacgaaacg gtgaagttgg tttagttgaa ggtttccgca tgggacaaat tttggatcac
    15181 gtttacgaag aagataaaga caaaaaagtg aaacggccaa tcatcgttgt gatcgatgta
    15241 ccaagccaag catacggcta taacgaagaa ttgatcggta tccatgtggc tttggctaat
    15301 agtgctgctg cttatgcaaa actccgtcaa gctggacatc cagttattgg attgattgca
    15361 ggaaatgcta tttcaggtgc atttttagca catggattac aatcaagccg cttgattgct
    15421 ttaaacaata aagaaattac cgtccaagcg atgtctaaag aaagtgctgc acgtgtgaca
    15481 aaacgttcgg ttgctgaaat cgaagcagct gctgaaaaag ttccagctat cgcttacgat
    15541 atcaataatt atacaaaact aggagcgatc tatcatttcc tagaagatat caccgaccaa
    15601 gctgcttcag aacaaaatgt cagcaaagtc atcgaagcaa tcaatgaagc cattcaagat
    15661 gtccgcaata caaatgacac gatgctgaca aatcgataca caaacgaaac agccaaaaca
    15721 ttcggacgtg tcgaaacaaa taaagtccgt gccaaaatgg acgaagaatg ggatgcataa
    15781 aaaatgattc aagcacatga tattgtcctt tttgataaac atgaaataga agggttcacc
    15841 ttgcctgaat gggtcgttct aactgaaaag atgttcggaa cagttcgtcg tgcgtcgacg
    15901 gatcacgcag aaatactacc gattggttta cgtggacctg cccgcaatca gagatggtca
    15961 acagaaatct caaaacatgc cgtcaaacaa gtcattcatc cgtgggacat catcgaacaa
    16021 gaatcgtata gacaagagaa aatcatggat tatcctgttt atcagcaatt tcatgctgct
    16081 agaaacatat tatcaggatg taaatgggga atcggcggca gcttaggatt tgaattatcg
    16141 actgggattc cggcagtaaa agaaaccagt gattttgatt tgctgctgta cgcaaattca
    16201 ccgattgaat tgccaattca ggtaattcaa tcgcatcctg ctttttttga gcaattcgat
    16261 acccaagtag tgactcggca aggcggtttt tcactaaaag agtatttgag agagccagag
    16321 aaaaaaattc tattgaaaac agtcgaagga cctaaattaa ccaaagaact ttggtaataa
    16381 agaaaaagag agagaaagag atgtttgaaa acaaaaaaat caaaatcaac gtaagtgact
    16441 tccagaaagt atcagctgta acggctgtta tgatgcaaac gatattgagc tttgctttgg
    16501 caaacagcca cagcgcaaca aatacagctt ttcttggtac cttttacgtc atcgttaaat
    16561 acacagcacc aatgtttatt tttgcaattg tttataatat ggtaaaaaca agtcagcatt
    16621 tgtcttattg ggaatttttg aaagaaaagt tttttgaact agtcgttccg tatcttctat
    16681 ggacgacggc ttatctttgg ctatttccag cagtccaaca acaaactccg tatacggata
    16741 ctgcaagctt tttactgaaa tatgtcacag gtgatggagc gcctcattta tggtataccg
    16801 ttatgatgct gcagatccaa ttgttcatgc cgttttttgt ttggctcggt tacaaagtat
    16861 ttgctaataa aaaatatgta tggccagtat tgattggttc aacgatttta tatgtcgcat
    16921 ggtatctgtt ctatcaaaca caagtattga ctgggcctta tcatgacagc tggtacttat
    16981 tagatcggtt tgttgctagt ttcatgattt atggggtagc cgcatttgtt tatcacgaaa
    17041 aagtctatca atatttggac agagtacgtt atcttttctt acctgttgcc ttagttatcg
    17101 cgttcttttc tgtccgttca ttgctcgcac atccaggaga cttgagcttt gcgaatgcac
    17161 cttacttgaa tacgatccaa agtttataca gtttagtcat catctttgct gtcttcatag
    17221 gcgcttccaa aatgattgta aacgattcgc caaaattgcc attgttcaaa tggttgtctg
    17281 tttatgctta tcgtacgtat ttagccaatg tatttgtttt ccaagtactg ttattattgt
    17341 ttaaagatag ttggctgcag ttaccttcag gagtcatgat tcttgtagct tatctaatga
    17401 cggcaagttg tgcatttgct ttaagctggt tacttcatat catttgggta gctatcaaaa
    17461 aaggtttcag caagtaatag taggaagaag gagtgagtgt catgataaat gacgaacgaa
    17521 aaaaagcagt gactcagcaa ctcgctgcgt tggtagagga agcattgatc gcagaagtaa
    17581 cattaagtcc aaaacctgga ttagtggatg cactggatgc gggtgcacat tccgatatgg
    17641 actatgcttt atttcttaag tctgctaaga cgctaactcc tttttttgaa gaaatggctc
    17701 aaatagcatg gcatcatgct attgatcagg agttaagaga aagaattgct gagattgggc
    17761 gaaaagcgga aaaggcaatg ctgattgcta caaatggcat caatacccat aaaggagcga
    17821 tttgggcaat ggggctattg atcagtgtca ttgcacaaaa aatcagtatg gaacagcctt
    17881 tttctttatc gaaaatactt gatgatgtca gcaagcttgc tgcttttcct gatacaaaat
    17941 atcgtcataa aaaaaccact catggtcata gggtgaagca aaaatatggc gtaaatggtg
    18001 catatgaaga agcgatatta gggtatccgc atatcaggct tgcaattcaa gcagctaagg
    18061 agctagataa tgattcatat gtacagaaac aaatgcatat gttgttagtt ttgatgagtt
    18121 cgctagatga cacatgcgta ttgcatcgca gtgatcagaa aacgttagtg gaaatgcaaa
    18181 aacttgctca tgctgctagt caaagtgctt taccaaatcg tccatttaca gcactgttgc
    18241 acttttgtca ggaaaaccaa atctctcctg gaggaagcgc agatctcttg tctgcaagct
    18301 tgttcatgct gaagatagag gaattatgga ctgccgacac atacatttgt ccgatgttac
    18361 tcaatgacta aataagacaa aattgctaaa aaaacagttt atgcatctgg ttattgactc
    18421 ttccttttag caaggctatc ataaaaatag ttttttgtaa taaaagataa cgaggaagac
    18481 aatgaaaaaa atcaattatt tagcaatcat tttgttagtg ggcctatgtt tgtttgctct
    18541 gccgacccaa ggattcgctg tcgcgggtgg tcatggtgga ggtacatcta gcggtcgaag
    18601 ttcgggcaga ggttcatttg gtggtcgttc atatgggggc agttattttg gcagtcgttc
    18661 ttacggcaga gattcttatg ggtattatgg tccaggtaca ggtccttcta gttatgagca
    18721 aacgggattt cttgcattag ctgttttgtc agtaggaggg gtttctgctt tttctatcgt
    18781 caagaataaa agacaaaaga aaaggctgaa agcggagata ttgtcccgca tgtcaggaac
    18841 ggctaaagaa aagaaagcac agtttctgga aatccaaaag atattcatga agatccaatc
    18901 cgcttgggaa gaagaaactc ctgaaaaagc cagaagctgt tatacagaca agctgtttgc
    18961 cgaacatcag aaagttctgc aaaaaaacaa agcagacggt gtaagaaatc atactaaaaa
    19021 aataactatt caggaagtag gaaattatcg acagatccac gaaaatagtt ttgccatccg
    19081 aattgatttc agatgctgtg attattcagt cgatcgaaaa gatgggcgat tgatctcagg
    19141 aagtaagaca caacgacagt atttttctca gatatggtat tttgattttt ctgagaaaga
    19201 aaaaagatgg caagccgatt ttattcagcc gattatgctg gattgacagg aaatatttat
    19261 gaacggagtt gtgacaaaag ccttgtcgca gctccttttt ctgaataaac ggtgtgccag
    19321 agatagccta tcggcaataa ggcacgattt tgtaaaatat gggaagcgat ttttcaaaat
    19381 cgcttcttat cgctcaaggc tgaacatctc tgtcacaacc tctccactaa atggtgttta
    19441 aaagcttgca tctgcgataa taagaacaac caaacgaaaa ataggaagca ctattttagt
    19501 ttagttgttc ttattacttg atgcaggaca gctttttcac aacctctttt ttgtatagat
    19561 gggcaaaatt tttgtaataa taggcgtatg tctttttcgt tgaggagatg aaaaagcata
    19621 tggcttattt tttgtttgct ttgcttactt tctttccact tcaagtggca gaaatatcta
    19681 ctttttatta tgtaagcgga accatatact tgaaatcaga aggagatcat ttctatgaaa
    19741 acaagaacaa ttgaaatact tcacagaatc atgacagccc aatatccagt aaaagcaaaa
    19801 catctagcaa gtgagttcaa tgtcagtcag cggacgatcc gtcaggaagt tcttgaagcg
    19861 aatatctggt tgagcagtaa aaagcttccg gagatccgta caattcgcaa caaaggtttt
    19921 ttgttgaagc tggaaaagga aaagcaagct cgattagaaa ctgaaataaa agatgtgaaa
    19981 gacggctttt tgaatcggcg cgaacgtatt tttgatctag ttttagccgt tgcttacagc
    20041 cgaatcccga tccatctaaa caaaaaagaa gaagaatttc aaatctctaa aagtacatta
    20101 gatgaagata tgagacggtt acgagcaaca ctggtcagtt atgggataga gatcgtcagt
    20161 tatggaaagc aaggactggt ttataaaggt tcagaacgat ctatccgtac gatgatctat
    20221 gattttatca atcagaatct gggaatgacc gacttcttta tgaatcaaga tgcacaatta
    20281 acaccggctc aaaaaatctt tctccattat tttcctttgg tggaaatcaa aaaaatacag
    20341 gagatctatt ccaataagtt gttgaaacaa gaagatgata tttataaaaa acaaatcctg
    20401 ctttttacat tgatctggat ccagcgaatc aggaaacatg aatttatctc tgccattaat
    20461 tggaaaaata cagaaataaa agacagtagc ttttctgcat ttattgacca catgatcgag
    20521 gaatttcaat tggacaatgt cccgcaagta gaaagaaatt atcttcgttt cactattgag
    20581 acgttcaatg ctagagatat ctccaattca atcgagtggg tacaagctca attactaacg
    20641 atccaattga tccaatttgt agagaacgag acacacgtac ctttccatct aaaggaagaa
    20701 gctttattcg aaaatctcta taagcacatg gctgcactga ttgtaagaat caagaatcat
    20761 atccaagtca tgaatccatt gaaagaaaac atccgaacaa attatttccc aatctaccgc
    20821 gcagtcactc attttgttcc tacgatcgaa gaagtaattg gtggcaagat actggaggac
    20881 gagatagctt ttcttgtcat ccacttttcg acgatcgcca gtgtaatcaa gcgagacttg
    20941 aattatgttt ataaatcagt cgttgtctgt aaccatggga tggctactgg aaatctgtta
    21001 gcagagaatc tgaaagaaaa gtttcctcag atcgagatta ctgcagtttt aagttcaaaa
    21061 gaaatcgaat tagtagataa attggatgtc aatctggttt tcagcacctt tcaattgaat
    21121 tatcgaaata agccgctttt agttgtcgat ccgattttaa ctgagacgaa tatcttggtt
    21181 atagaagact ttttgaaaag aaatgaagaa ctacagcggt tagagcctaa cagtcatgaa
    21241 tcgacggagt tattcactca aataatagat gtcatgaaag aaagtggtgg ccgagtagat
    21301 cgaatcatct atggcaagtt agaaaaatta ttcgaaacaa acaatttaga aatcaacaaa
    21361 agggagatcc agcctatgtt aaaagatatt ttgacggaca gcaatatcat gatcaaagaa
    21421 aaagccacta cttgggagga atcgattgaa ctagctgcta gaccattggt taaagaaaac
    21481 attgtcgaag agcgctatgt cgaagcgatg attcgtgcag tgaatgatta cggaccgtat
    21541 atcgtgattg gcaaacacat ggcactagcc catgcccgac cagaagatgg ggtcaataaa
    21601 ttaggagtca gcgtagctac gattgagcaa ccaatcgatt tcggtaatcc tgaaatggat
    21661 ccagtaaaga ttattttttg tttggcagca gtagactcct actcgcatct gaacatcatg
    21721 aaagaactaa ttgaattgat caacaacgaa gagaagttgg atcgattgat tgaatgcaca
    21781 aatatcgaag atttcaaaca actgcttttt tcagtagttg agaaataata aatgatacgg
    21841 gggagaacaa agatgaacaa attgaacatt ttattcgtat gcggtgcagg tttaggaagt
    21901 agttttgcag cacagatgtc tgctgaggat gttttgaaca aattaggagt agaagccaaa
    21961 ctggaccatt gtgatatttc ttccgcagtt tccatgaaac cagatgtgat catcacagca
    22021 gaaaattttc gttcgcagtt cgaaaagttc gcagttgatc ctaaaacggc gatgatctat
    22081 ttgaaaaata ttgtttcaaa aaatgaaatt gaagaaaaaa tcaccccagt tctgcaagat
    22141 aagggagtta tctaaataca aaaagaaaag agggtaaaaa
//
LOCUS       DAYYCW010000047        22028 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000047 DAYYCW010000000
VERSION     DAYYCW010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.047"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..400
                     /locus_tag="ACHAYA_001558"
     CDS             <1..400
                     /locus_tag="ACHAYA_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8605358.1"
                     /translation="EPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYY
                     FVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNNKI
                     KLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(783..1511)
                     /locus_tag="ACHAYA_001559"
     CDS             complement(783..1511)
                     /locus_tag="ACHAYA_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8605359.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1489..3063)
                     /locus_tag="ACHAYA_001560"
     CDS             complement(1489..3063)
                     /locus_tag="ACHAYA_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8605360.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            3292..4230
                     /locus_tag="ACHAYA_001561"
     CDS             3292..4230
                     /locus_tag="ACHAYA_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8605361.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            4242..5153
                     /locus_tag="ACHAYA_001562"
     CDS             4242..5153
                     /locus_tag="ACHAYA_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8605362.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            5177..6649
                     /locus_tag="ACHAYA_001563"
     CDS             5177..6649
                     /locus_tag="ACHAYA_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8605363.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6700..7311
                     /locus_tag="ACHAYA_001564"
     CDS             6700..7311
                     /locus_tag="ACHAYA_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8605364.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            7341..9254
                     /locus_tag="ACHAYA_001565"
     CDS             7341..9254
                     /locus_tag="ACHAYA_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFM8605365.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            9382..12363
                     /locus_tag="ACHAYA_001566"
     CDS             9382..12363
                     /locus_tag="ACHAYA_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFM8605366.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            12360..14021
                     /locus_tag="ACHAYA_001567"
     CDS             12360..14021
                     /locus_tag="ACHAYA_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFM8605367.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            14061..14993
                     /locus_tag="ACHAYA_001568"
     CDS             14061..14993
                     /locus_tag="ACHAYA_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFM8605368.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(15385..16932)
                     /locus_tag="ACHAYA_001569"
     CDS             complement(15385..16932)
                     /locus_tag="ACHAYA_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM8605369.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(17034..17387)
                     /gene="tnpB"
                     /locus_tag="ACHAYA_001570"
     CDS             complement(17034..17387)
                     /gene="tnpB"
                     /locus_tag="ACHAYA_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM8605370.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(17377..17571)
                     /locus_tag="ACHAYA_001571"
     CDS             complement(17377..17571)
                     /locus_tag="ACHAYA_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605371.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(17797..18999)
                     /locus_tag="ACHAYA_001572"
     CDS             complement(17797..18999)
                     /locus_tag="ACHAYA_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8605372.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(19002..19196)
                     /locus_tag="ACHAYA_001573"
     CDS             complement(19002..19196)
                     /locus_tag="ACHAYA_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8605373.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(19243..20091)
                     /locus_tag="ACHAYA_001574"
     CDS             complement(19243..20091)
                     /locus_tag="ACHAYA_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFM8605374.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(20296..20646)
                     /locus_tag="ACHAYA_001575"
     CDS             complement(20296..20646)
                     /locus_tag="ACHAYA_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605375.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            20824..21687
                     /locus_tag="ACHAYA_001576"
     CDS             20824..21687
                     /locus_tag="ACHAYA_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8605376.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
BASE COUNT         6920 a         3664 c         4302 g         7142 t
ORIGIN      
        1 ggagccactc aaacggcatt atcatcgact gtttaaacga ccaattagtc aaacagaaat
       61 cgttgatgag ctgttaagct acaacgaaga attgcgaact gcgtatcatt tttgtcaatt
      121 attacgctat tatttcgtta aacgaggtac ggaaggcttt caagaaaccc ttaaaacaat
      181 ttcatctacg ttacctcagt cgtttaaaaa gaaattcacg atcttccatt gctaccaatc
      241 gggtatttca aatgccttta aggtgtccca ctcaaacggc gtcactgagg ggctgaataa
      301 caagatcaag ctgattaaac gaattgcgtt tggttatcgc aacttttata actttcgggc
      361 acggatctat ttacaacaag gtctgatctt tgaaaactaa aaaaatatgc ggcgcacaga
      421 acgcgccgca tatcggacta agttttattc aaatttgtct tcaccaacac gatttgacga
      481 agagcctatt taaataacta catccattta ttctatcttc taaaatagcc tttttcattt
      541 cagttaaata gttcagcgcc atttttgcac tcgacttgca tagtagtttt ttccatattc
      601 ctcaacctca gcgggggttg aagacataag gaaaatccta tgcgttatgc tcaactggat
      661 ctgattaaat attctaatgt atttttctct accatcatta aatggttaat agccaaaaat
      721 caccactttt caatggctcc ttgttgtata agtataacta gaagatgaac acgtctgaga
      781 aattatagaa gctttttacg gtaattactg gggctgtgtc cagtagtttt tttgaagaga
      841 acagaaaaat agctcacgtg atctattcct actttttgac taatttcatc aatagataag
      901 aatgtgtctt tcagtagagc acttgctctt ttgattcgaa agtctagcaa gtaatcatga
      961 aaggaataac ctgtttcttt tttgaaggcc cgtcctaaat aagcgggatg gtaatggaat
     1021 tgtgtagcta catctttgag cgtaagcttt tcactataat tgttttgaac gtaatcaatc
     1081 acttgttgaa ttgactcatt aaaagaatca tcagttagag agttttgatt agctgctgat
     1141 ttacgcattt tttcttcttg tactaactta actaaataag gaaaatcaat gggtttcgtt
     1201 atgtaacttt taatagataa atcaatcccc ttttttgcat actgaaaatt ttggtgggca
     1261 ctaatgacaa tactagtaaa gtagcgattg gatgtttgtt gcaaagattc gattaattcg
     1321 agtccagaaa ttttccccat cgtaatatct gtgataagta aatcaaagcg ctggttttgc
     1381 aggtttttaa atgcttcgtc tccgtctgtg tagcagtgat cgataatgat gtcggggact
     1441 ttcttcaaaa gataatgcct tatgccttct acaattgtac aatcatcgtc aacaattgca
     1501 attctcaaca tgcatattcc cccctttttg tattttgata gtaatcgttg ttccgacgtg
     1561 tagttggctt tctataacaa aacgtgtatc tccaggatag agatgcttta tacgtcgaaa
     1621 agaatttgag atgccgatat tctcactata aggaatatca tcattaagtt tttgttgaat
     1681 atgctccagc ttgtcttggt ccattccttt cccattttca acgattttta acacataatg
     1741 gttggttgtt tcagttgcat agacagtcaa acgattttcc ttttttgatg aatcgaagcc
     1801 gtgtttaata taattttcaa caaatggctg taaagaaagt tttggtaaca ttaattgatc
     1861 aatatgatca atcagctgat agtcaaagtt atgtcgataa cgacgttgat gtagttgcaa
     1921 ggagaggtcg atattaatta attcctcttt caaatcaatt atgtcgggct gatatgtatt
     1981 attgcgatac aacttgctca ataaatatat actgtcgctg atttcttcgt catcattcaa
     2041 tatggcagtc attcgaataa attccaaatt attaaataga aaatgaggct cgatttgata
     2101 ttgaagtgct gcatactttg cttcttgctt caaccaatta ttttcataaa gtgttttttg
     2161 atatgtagca ttatctaaat aaaactgatt gatatgcata gcgatttctt tcaaatcacc
     2221 gactttgttt tcgatattga ttgacttgta gtctcctgta gatgaatgat ttaaggaagt
     2281 aatgatttcc tgatactgct ggatatagtt acgaaaactt ctatttaata attgatgaat
     2341 agcaagaata aacaaactca ttaataagaa aatcatgatt gccagaccta ataaggtttg
     2401 tgacgaataa tttggatttt catatacttg taatgtgtgg ttaccgatag ctatctgttg
     2461 cgtttttttc catttagaag gcgagatgtc ttcagataaa ttgacggaat caataccatt
     2521 agacaaagaa taattgtaat gtgttggaaa gagactcttt tttagcgcgt tgatcgaaac
     2581 gtctagaatc aatgtagcat cgggcatgtc ctcactaatg aaacttaatg aacgaataaa
     2641 agaaattttt tcgttttttg taggtgcgta ggcacttaag tatttttcgt aatctaatga
     2701 gatagatata tttttaactt catcatcttg cacaagtttt tttaagttat ctactaaata
     2761 ggtaggggag cgatcctttg acaaaatatc ttccatgtat tcagcaggtg atgagaggta
     2821 gtagtcatta aggttttctt ggaaatcata attttgatag atctctgtca cgataccgga
     2881 gagaatacga ttatatgttt ccatatagtg ggtgatttgc tgaatgtttt gcaggttttg
     2941 atttttttct ttttcaatca cattgatata ggaaaagaac aatgtgaaac aggcaagtaa
     3001 agtaataaaa aaaatcaata tgtatgtaaa acgcataata acttttgata aatcgttttt
     3061 cattcaaagt cctaattttg ataatctcta attttatgaa tcattatagc acatattaca
     3121 agtgatgtaa acgctttgca aacagggcta cttatgattg tgaatacagt ttccgtttaa
     3181 aaactattct attgtagagg tttaataaac taaaaataat gtataaaaaa ttaatgtaag
     3241 cgattaacca agtgggtata atcaaggaaa cgctgagaga ggagagacaa aatgaagcta
     3301 aagaaagggt tattaggaaa tctaaaaaaa aaccgagcat tgtttattat gcttttacca
     3361 ggtgcgttat ggttatttat attttgctat ataccaatgt ttggagttgt aatcgctttt
     3421 aaagatttca gaatttcatc aaggggattc tttacaagtt tgttcaacag tccttgggtt
     3481 ggctgggaaa atttcaaata tctctttgct tcgcaagatg cttggatcat tactcgcaat
     3541 acggttgttt ataacgttgt aggtattttg ctaagtactt tttgtacagt gtcattagca
     3601 atcattctga gccaaatggc gaacaaacgg ttattgaaaa agatgcagac atttatgatt
     3661 tttcctaatt ttttatcatg ggtagtcatc agttactttg tttacgcatt tttgagtacg
     3721 gataaaggat taattaatat tttactaagc aattttggtg tggaacccat aaattggtat
     3781 agtgaaccga agtactggcc ttttattatt gtttttacga gtttatggaa aggcgtaggc
     3841 tatggatcca tcatttatta cgcgtcaatt atggggatcg atactagtta ttatgaagct
     3901 gctttagttg atggtgccag taaatggcaa cagataagga atgtagtctt gccacattta
     3961 agatcgatta ttgtcattat gttgattttg tctgttggta gtatttttcg agctgatttt
     4021 ggattgtttt atcaattgcc aagaaattcg ggggctcttt atgatgtgac gaatgtatta
     4081 gatacttatg tctaccgtgg attaatgaat agcggagata tcggaatgtc tacggcagcg
     4141 ggattatatc agtcatcagt tggctttgtc ttgattttga ttactaataa gatagtcaac
     4201 aagttagatc ctagttcagc actattttag gaggaatcaa aatgatatca aaattaaaac
     4261 ggcagatgta cttttcgctg ccagtcgaac ggtttttcac aatattacta atcttattta
     4321 ctcttagctg tatttttccc tttattttcg taatcgtcat ttcattaaca gatgaaaaag
     4381 cattacaact caacggttac gaattattgc cggctcaatg gagcactgat gcatatcaat
     4441 acttaataca agatggcgga cagttacttc ggtcattagg cgttacaatt atgattacag
     4501 tgattggaac gttgattact gtttttatga cgggaaccta tgcttatgtt ttatcaagag
     4561 cctctttccc ttaccggaag ttctttactt tttatctttt ttttaccatg ttatttgcgg
     4621 gagggatggt tccttcttac ttagtgatga cgaagatgct tggcttaaaa aatacaattt
     4681 gggctttgat tctacctctt gcttttagcc catacaatgt cattatttta aggacattct
     4741 ttaagaagtc gattccagaa tcgattatcg aatccgctaa aatggatggc tgtagtgagt
     4801 ttcgagtttt tttccaaatc gttttgcctt tagcgatccc aggggttgca acaataggtt
     4861 tattttcaag cttaggttat tggaatgact ggttcaacgc attgctgtat attgacacca
     4921 acaaattgat tccattgcaa tatttgttaa tgaagattca aaattcaatg gaattcttag
     4981 caaataataa tgacattaca ttagcgcagc aacaagcaat tcaaaatagc cttcctcaag
     5041 aatcaacacg aatggcaatg gtcgtagtag ctactttacc gattgcaatt gtttatccgt
     5101 tcttccaaaa atactttgtt caaggattga cgatcggtgg agtgaaagaa taaaaaagaa
     5161 taggagatgt taaaagatga aaaagaacat gcagaaatat ttgattggtg gtgcttctgt
     5221 actaaccctt ggcctattag gtgcttgtgg tagcagtaat ggcgaagcaa ataatgaaac
     5281 aggaaccaca aaagttactt ggtatcaggt tggagaagaa cccaaagatt ttgatgaggt
     5341 catgacaaaa gtcaacgaaa aattaaagga agaaatcaat gtagagttgg atatgcggct
     5401 gattccggac ggggattatc agcaaaagct tggtgtgatg atcaattcag gagaagaata
     5461 tgatatttgt ttcgtcaatg gcacagatta tgtgaattat gggaacaaag gtgctttcat
     5521 tagtctcaac gatcatttgg ataaagagtt gaaagattat gcagctgaat tgaatgaagg
     5581 atttattgaa ggtggggcaa ttaatggcga aacttatgga ttgccagtca ataacgatat
     5641 tgccacaacg gaatattgga cctttaataa agatttggta gatcaatacc aattggatgt
     5701 agagaatgtt gaaggtgtag aaacattggc tccagtatta gaaaagtttg cagaaaaaaa
     5761 tactgatacg aatattacgc caatcggtat gacccgtacc agtgtctgta ttccttatga
     5821 caatattatc ggtggtggtg taccgctagc ggttgcattt gaaggcgata cacaaacagt
     5881 tgttaatact tatgagactc cagaaatgat gacttatttg aaggctatgc atgagtttta
     5941 taaaaaagga tatattttga aagatgcagc cactgttacg acagatccat ttcgaattga
     6001 tagtaaaaac tggtttttag gtcgtaactc aagtggttat caaccattag cacaaaaaaa
     6061 attagaagct ctagcgggac atggattaat tttaaaacca gctggaaaag cttttaaaac
     6121 gactggattt gcaagagtgg caatgcatgc ggtatctgct tcaagtaaac atccggaagc
     6181 agccttgaaa ttgttgaatt atgtgaatac caatcaagaa gtagcggatt taatggttta
     6241 tggaattgaa ggaaaacact atgatcgcaa tgaagatggt tcgattactt taacaaactc
     6301 tgatgattat tatcaaggcg cttgggcatt ggtaaatact tatctagttt caaaaacttc
     6361 accgattaca gaagaaagta aaaaactaga agaagaaagt aaaaagtttc aaaatgaagc
     6421 accgatgtct cctattttag gtttctcttt tgacgcttct gattttcgaa cagaaattgc
     6481 aagtgtacaa aatatcgtgt cacaatatga agtactttta agctcgggaa cagttgaccc
     6541 tgaacaatat gtaccagagt tgttagaaaa actagaaaaa gctggattgg ataaaattca
     6601 ggcatctgct caagaacaat acgatgaatg gcgaaaatct aacaaataat tttttactaa
     6661 atgaaaagaa tagactaagc agttagtggg gggattcaaa tgaacgaaaa caaatggacc
     6721 aaaggattta attgggtttt tcaattgttg ttactaaact ttacttattt tatactaatt
     6781 tgtttaggat ttggaatttt aggttttttc cctgctacga tagctttgat tgctggcgtt
     6841 gtgaatcgaa aaaaatatgg cttgcaagag tctgtgaagt tcatgagtaa aatctataag
     6901 aaaaattttg ttagatataa tctatattgc tggtcagcaa ctttgttgtt ggccggcaat
     6961 attactacga tctggctttg ttggcagcaa agcgcaccat tgttttattt attaagtaca
     7021 ttattatcgg taattacact gttcctagtc tttgcaatgt tgaattgggt cgtgatcaaa
     7081 agtcttgggt tgtggatgat gggagaccga ctgattgact atttgctttt cacagcaacg
     7141 atgttgccaa cggtagtcat gcaaggcttg gtgcttggaa tttatttccg aatattgatg
     7201 attttgcctg tgatttttct gtttggcgga acagctgtaa cggtaatatt gattagtaaa
     7261 ttaactgaaa gagcgttagc aaagttgcct tgtcagatac aatcgcacta attttttcac
     7321 aaaaaggagt atttctttct atgaagttaa ctgataaaca agtagctaat atagccttta
     7381 tccagcaact tgagattcca ttgacacaaa aagatgaaga acaaatagac ttgattttta
     7441 ctgacacaaa ggctaatgtg cgacaaatca agattactaa aaatgagcag caacgaattt
     7501 gcttgtctta ttcacatgaa gacttgctgt atcgaggatt gtttatctgc cttccacgtt
     7561 ttttagctca aggaggagag atgccataca ttgaagaaac ctcgatcatt gagaccagtt
     7621 attcattaga tgcttctcgt aatggcgtga tgaataaaga gactctaaaa aaattcattt
     7681 cttacttagc gttgatgggg tacaacacat tgtatattta cacagaagat acctatgaag
     7741 tagaagacct tccatatttt ggttaccttc gcggagcgta tacaaaagaa gaagtgaatg
     7801 aaattgttga atttgcttct ggttttggca tagaagtagt tccatcgatc cagacccttg
     7861 ctcacttgac ccaatttttg aagtggtatg ctcaacaaga actgatggat gatcaaaata
     7921 ctctactgat cggtgaagaa aaggtttatg cggcaatcga aaagatgatt attgcctgca
     7981 aagaaatgta tcagacagag cgtattcatt tgggaatgga tgaagcctat caagcaggtc
     8041 tgggacgata tttaagcctg tatgggtatt gtgatcggac aactttaatg attcaacata
     8101 tggaccgtgt gttatcaatt gttgataaat ataagatgaa accaatgatt tggagtgatt
     8161 tcatttataa gctattagat gaaaccggta aagataggta ttacaatcct gattttaaac
     8221 tcaaagcatt agcagctaaa ggattaccag caggattaac ctatgttcat tgggactatg
     8281 gttcagatga agtttcctcc tatgagaaag taattgataa ccatttgaaa ttttgtgaca
     8341 ttgatcatta cgttttagct agtggtgccc atatctgggg gagactagcc ccgaatcatg
     8401 ggaaaacaga agcaattgtt tctgcagctg taaaagcttc taaaaataaa ggtgtacgtt
     8461 ctgtgatgtt aactacttgg ggagatgaag ggcaggaaac agagcattgg cattccttaa
     8521 ttggggcatt aaaattagca gattatgtct attctgaaga agagtggaat gattttgagg
     8581 aaatctacga tcgtttatta ggaacaggta gttatcaatt aatgtccgaa ttacgatatt
     8641 tcgatgaagt ggataccatt acaaataaca acttacaaat ggcttccgtg agtaaactgc
     8701 tactttggca agatccgtta gttggtatat atgactttca tgtagccgct cataacaaaa
     8761 ttgccactag aacaattggt acttattatc atgagttagc tcagaaatta gatatgaata
     8821 aaaattggaa tccaaaacaa ccaattttaa caactattcg tcaacgatat atacttttag
     8881 ctgaaatttt agcacaaaag gcagaactag gcatctattt acaacactgc tgtctggtag
     8941 aagaccttgc aggattaatg gaattagcga atcggtgtga agcactagcc aatcgagtaa
     9001 atgaattggc atctattcat gaaataattt ggtatcaaac atacaaaacc tatggttggg
     9061 aagtattaga acaacgatat gctgcattag ctcatcgtct gaaaaccgtt gcgcgtcgta
     9121 ttaaaaatca tcttcagggg caagatgccc tagaggagct tttggaagaa cgattaccgt
     9181 tttgtcaata tcaagtgccg cttgaaattt ctggctttaa ttatcggcaa agctcggtgt
     9241 caggctacaa ttaacgaagg aatcttcccg ttgaacggac cacttgctca tctaatggga
     9301 aagtggttcg ttttctattc ataagtagct taccactaac tcttttatta agaagagaaa
     9361 aaaagtagga agtgagaaaa tatgttatcg gactattatc aaaatctgtc agttataagt
     9421 gtcaatacag aaccattacg taattattat attccttatg caagtaaaga agaagcaaaa
     9481 caagagttag aaagggaaaa aacgagccag tttatcgatc tcaatggtac atgggatttt
     9541 cagtattatc cttctgtcca acagttaccg gaaaattttt ttcaagaggc ctttactgaa
     9601 agctatgata gaatttctgt tcctggagtt tggcaaacag agggatttga cttacctcag
     9661 tatatcaatg tatcttatcc aattcctttt gatccgcctt atgtacctaa tgatacccct
     9721 tgtggtgtat atcggcgtca gtttcaaatt gagcaaacag atgcacgctt ttacttaaat
     9781 ctagaaggag cagatagtaa ttgttttgtt tggataaata gaaactttgt aggctatgac
     9841 caagtgagtc atagtacatc tgaatttgat gtgaccaagt atttacaggt tggggttaat
     9901 gaaattcaaa ttctagtgat gaagtggtgt gatggtacct actttgaaga tcaggataag
     9961 tggcgaatga gcggtctatt tcgtccagtg tatcttcttc aacgaccaac tatgcatctg
    10021 acaagttatc aactacggac gttgactgat cctaaaaaag gaagcggaac attaatggtt
    10081 tcttttcaac gatttactcc tattgaagta tcgttaccta tttcttacga attattccat
    10141 cctgatggac gtctcatagc gaaaggggag tgttactcgg aacaacaaat aaatttggat
    10201 gttgaagatt gccttttttg gacagcagaa acgcctaaat tatatacatt acttttaaca
    10261 tgtaatggtg aatattttaa agaaaaagtt ggctttcgag cactgacgat caagaatcgt
    10321 aagttattgc tgaacggaac acctattaaa ttatatggag tcaactatca tgacgtgaat
    10381 cagcaaggca cgccagtgat gacccgagag cagttacgtg cagatttgtt attaatgaaa
    10441 aaattaaatg tgaatactgt acgaacggca cattacccta aatcaccaga tttttatgaa
    10501 ttggcggatg agattggtct gtatgtaatc aatgaagcag atttggagtg tcatggtgtg
    10561 gttaatctat atgatactga agcgaattat caactaattg ctaatgatcc gacattcgaa
    10621 aagccgatta tggaccgagt gatccgttct attcttcctt atcaaaatcg aagctctatc
    10681 attatgtggt ctatggggaa tgaatctggt tatggagtaa attttgaaaa agctcaagct
    10741 ttagcacgga aattggatga ttcacggttg attcataatg aacgctcgat tgaaccagtt
    10801 attgaccaag tgaatgattt tagtaacttg gatgttatta gtcaaatgta tcccccactg
    10861 gaagatgttc atcgtcattg tcaaagaagt gatactgaaa aacctttttt tatgtgtgaa
    10921 tatagccacg ctatgggcaa cggtcctgga gatcttaaaa cgtacttcga tttgattcaa
    10981 cagtatgatt cctttcttgg cggttgtgtc tgggagtgga ctgatcatgc aatcaataga
    11041 ggaactttag aaacgccatc ttatttatac ggtggtgatt ttggtgaaaa atatcacgac
    11101 ggcaattttt gcgtagatgg tttattgacc ccagatcggc aattaacagt aggtgcattg
    11161 gagtaccgta atattcattc gcctattcaa ctgattgctg tagatgagga gctgcaagaa
    11221 gtaaccttga gcaatataac tgcttttaca gccggttcag catttaacct ttctcttgaa
    11281 tggtatatag atggaaaaaa aacagtttat gaagtaatga atcctttcat acttgagcct
    11341 ggcaaaagtt gttcattgcc catcacagta cctacaacga ttcatggtgc tcatacggtt
    11401 gctgtttcgc tatctgttta taaaaatgat gagctttatg gacagcaaca gctctttctt
    11461 aaaaaagaaa aaatgagatt gccaagaact gctcatcata atgatgcacc acaaatttca
    11521 gtaaaaggca cagagctgat aatcaaagga gaatccttta tttatatata tcaattagca
    11581 aaagctgcgt tcacttctgt aaagatcaat caaaaggaac ttttggaacg tcctactcaa
    11641 ttttcaattt ggcgtgcacc aacggataat gatcgtaact tagtaaacaa atggaaagca
    11701 gctggatatg ctgaaatgtc tttagaaatc agaaaaaaag actggaaatt cgtagatgat
    11761 catctcctga tcgaaacgtc ttatcgagga gttattccag gattacagcc aatttttaca
    11821 atgcacatac agttgaaaat aaatggtgca ggcgtaatca accaaaaagt atcagtggag
    11881 aaaaatccga tttttccaga tttccctcgc tttgggatgg actggccgtt gaatgatact
    11941 ttttcgaagg tttcttattg tggctatggg ccgtatgaaa gttatcctga taagcactat
    12001 gctgcgatgt ttggtattta tgagatggag attgaggaat ttgatcagcc atatatcaaa
    12061 ccacaagaat atggtaaccg ctggcaatgt gattgggtat caatcaaaaa tcatcaagga
    12121 tctaccatga cggtttatgg aaactctaat ttttctgctc tgccttatag tcaagctcaa
    12181 ttaaccgaaa agtcacacca ttatgaatta ataaaagaaa atagaacttg gttcaattta
    12241 agttataaac aaaatggtat cggctcacac agttgcggtc cttatttaga agatcgtttc
    12301 cgttttatgg aagatacttt tgactggaat atagtgatta ctttcaaagg agaaaataga
    12361 tgaaagaaac aaagatcaat caagtcaaaa aaaaattatc tcgttatcaa gaagttgttt
    12421 atcgaaacca ttgcttaaaa tggtcaaaaa ataagctgaa tgttcgtttt aatggattat
    12481 tttatcgttt cgaacagtta gaagaagtct ggaaaccggt aacgaatttc caatcatgtc
    12541 ctgtacagct cataaatgat ccaaaagaaa catacgattt atgtataaca cacctttata
    12601 accagcattt gaaaaatgac ggattttatt taaaggttac taatgacaat caacttacga
    12661 ttgaatcagc aaaccttcga gggtttcaat atgctatgga agccttatta gagagttttt
    12721 tttcggtagg agatcaatta ttattaccgg aattgattct gaaacatgaa ccatcagtga
    12781 aaattcgagg aatcattgaa gggttttatg gtatcccctg gacacatgaa atgcgcctct
    12841 ctcttttttc atatatgaaa gataaccaat tgaatacttt tatgtatgcc cctaaagatg
    12901 atgaattatt acgtaaaaaa tggcgtgaac tctacgatgc gcaagaatta gacaaattta
    12961 aggagctttt atctgctgct gaggctagca atatcgattt ttggtactta attagtcctg
    13021 gaaatgatat tgatattacg tgtgaagaag acattcaagt tcttctgaaa aagttagaac
    13081 aactgattga gcttggcgta tttcaatttg gtctattaat ggatgatatt gactatcaat
    13141 taaaaggtgc tgctaaacgc cgttttcgag agccagcatt cgctcatgcc tatttagtga
    13201 atcgagtaga ggaatatctt agcactcggt tgtccaatca tgaattagtc atttgtccta
    13261 cagaatatga taatcgtcat ggttctcgct atttagcaac gttaagtcaa gaaataccag
    13321 aacaattacc gttcttttgg actggcccaa gcacgttagc ggcgtcaatt tctacagaag
    13381 agctgcagga aatggcttct gtctatcaac ggccgatgct gatttgggat aatattccgg
    13441 taaatgatta tttagaggat aaagaactat tgttcatgag tccatatgag aatcgaacac
    13501 cgaatctttc taaggagaga taccaggtta caggagtagt ttcgaatcca atggctcaat
    13561 tagaagcttc aaagtttacg atcaatagta tggctaatta cttatggaac tgtgagcgtt
    13621 ttgatccttt ggaaacttgg acgtcagtcg ttgaaaaagt agttggaagt aaactacaac
    13681 cagcttatct taccttaacc aatgcttttc caaatcatta cacaagcagt ctgctttcag
    13741 atgaagaatt actgctagcc aaagacccag aatgggtcac aaaagaaatg gctgctttag
    13801 tccaagcagt aaaatggatc cagcaagaac aacatacatc tagattgaga acagaattgg
    13861 agccttggtt acaagcaatc acagaaagtt ggactttctg gcaagaatgg caattaaaaa
    13921 aagatccaaa agaagcagaa gaatggttgg ctaagaaaaa aacacaccgc ataggacgtg
    13981 atttagtaac tgcccattta gagcaaatta tcaaaagtta gatatcttaa aaaataaaaa
    14041 gcaagaaaaa ggagaacaaa atggtcaaga gattaattag tgcaaactac agtgatgtca
    14101 agaaaatgac gtcaaaagag ttgaagcaat ccatcaaagc gagtgaagga cgggtgattg
    14161 tttgtgaaac ggttgctaat actcttccac aatctggaga aattacgaat gcagaagttg
    14221 cgtcagcatt tggtgcagat ttgattcttt taaatggttt cgattgttat cgtcctgcag
    14281 tttttggact accaaatgtg acgattgaga aggtttttga tctacagcaa acgattgaaa
    14341 acccgattcc taaattaaaa gaactagtcg gtcgtcctgt gggaattaat ttagaaccca
    14401 ttgcccctaa aacaaatatg atgagtgaga aagtagaaat cagccaagga agagcaagct
    14461 ctgttgaaac gatacaaaaa gcagaagaac taggagtcga ttttatttgc tttaccggta
    14521 atccaggcac tggagtaacc aatttagaaa ttactaaatc cgtaaaaatg gcaaaaaagt
    14581 atttttcggg attgattatt gctgggaaaa tgcatagcgc cggctcagaa gagccagttg
    14641 tgaataaaga ggcgattatt tcttatgcgg aagcaggcgc agatatttta ctagttcctt
    14701 cagtagggtc tatcccaggc tttttagagc aagacctaat tgaagcggtt cggttggcaa
    14761 aaaaatatga tttgttaacg atgtctgcaa tcggtactag tcaggaaagt gcccgaccag
    14821 aaacattgcg gcaattagcc attatgaata aaagttgtgg tgttgattta caacatattg
    14881 gagatgctgg atacggggga ttggcaccag cagaaaatat tttcgaaacc tcggttgcaa
    14941 ttagaggaat gcgacatacc atcaactgcg ttagccgctc gatcaatcgc taacaaaatt
    15001 aaataaaaaa tggttaaatt gattatttaa gtgggaacat acttgagata tcagctaact
    15061 tctgtcactt taaaaaaggg actgtgacag aagtgaaaat agacaaaaaa tatccgaaca
    15121 agagactagg atagcagaaa aactatcgtt ttgcaacgag gaccaactgg accgtaggag
    15181 caattctctt attgttcgga tatttcgctt gttcaaggac ttatgtctgt agtgcccttt
    15241 tttggtagga aaaatggtag ggaaccatat catttttaat gaaaatcaag catttcaaaa
    15301 gttgaaaagt aagcgcccca tagaacagta cctgtacgtc aaataacccg acctatagaa
    15361 tagaatcggg aatgtttggc gtttttagtt acgagaaaaa tgttgttgta catccttgtt
    15421 ccaaggcaac agttgatcta aagtttctgc tgaaagtacg ggaagatttg gtatcttatc
    15481 aaatagaaac tctagatact ttacaggatt caatccgtta agttctgctg ttttatatac
    15541 actcagaatg attcctgaag ttcgtgctcc ttcaagactt gtggaatgca accaattttt
    15601 ccgtcctatc accagctctt tgatatttcg ctccgcacga ttatttgtga gttctaaacg
    15661 accatcttca aaaataggtt caaaaccact tcgtagttta cacgcatatt gaacggctgc
    15721 atccaacttg gattttgcaa caggatctat cgttgccatc cactcataaa aagcaataaa
    15781 taatggacgc agttcttgtt gccgacagct taatcgcttc tcaggagaaa agtctttcca
    15841 tttcttttct aggacaaacc atttatccaa ttgtttgact gcttgagctg cgggaatatc
    15901 cgtgtttttc tttccttttg gaattgcttc gaaaaatttc cgccgaacat gcgcgaaaca
    15961 atagacaagt cgtacacttt ccacggcatt atacccgcta tagccgtcac agtgtaaata
    16021 ccctgtaaac tccttgagaa aagttttcgg aacatctcca gcacgacttt cgtcgtggtg
    16081 ataataaacg atgggatgct gactgtcttt tcctgtacaa aactgccagt aataggtatc
    16141 tgttttggca ctttcaatca cacgataact tgtttcatcc ccgtgtagaa tctcttcttg
    16201 attaagatag actcttaatc gttctcctaa agggtctaac gcgttttgca ctgctttaat
    16261 atgccaattg gtgatattat ctctcgaaat atccaaccca tgtgtttgtt tccaatagtt
    16321 ctcttgccga taggcaggaa ctttctgacc aaatttcatc cggatcgttt ccgccactaa
    16381 actattggaa cctaagctat tagaaatcaa aggtttagga acgatcgctt ttttaaaaga
    16441 agaaagtcct tctttttcgc agcttttaca acaataggta tgttgataat gggtgagaca
    16501 atccaacata gcctgatgga aacgaatttc ttctcgtact tttgtccgtc cgatatcttt
    16561 catctgtgaa ccgcaatgtt cacaaaattg ttcttccaaa ggaaggagat gttctttctc
    16621 tgttataggt aagtgtttga ttttgtctgc tttcgttcct acaactcgtt gatgtccttt
    16681 tactggaata atggtggcag ctgcttctgg aacacttaca gaaatttctt catcactaaa
    16741 aagattcaat tgcccattcg tttcctcaaa gagagtctct ttagagcgtc caaataattt
    16801 gttggttaaa taattgacct tttcattcaa tagttggatt tcagttgtaa gattctgaat
    16861 ggtttgttgt tgtgcttgat tggttttatg ggcttcttga agcaactgaa gcaacgtatc
    16921 tgtagtttcc aaaatttttc accaccttga atcgaactga ttttgttaca gaataccaca
    16981 agaaacgcac aagaaatagg ggttcaacac attttttgtg ttaacccccc agcttaaaaa
    17041 actccttttt ttcctggcaa aatagctcga ggttgatcga tacttaatcc ttctaacaac
    17101 catcgaacct gttggttcgt taaggttcga acttcgtctt tcgtccgagg ccattttaac
    17161 ttgccgtttt ctatccgctt ataacagaga agaaaaccat caccgtccca atagagaagt
    17221 ttgaaacggt cttgacggtt cccgcaaaat aaaaaaacgg catcttcgta aagctcgagt
    17281 tgatactctt gttgaataag actggcaaga ccatcaattc cttttcgcaa atctgttttt
    17341 ccgcacacaa tataaatatg tttggtttga agtaaatcat aaagcattgg acaacagttc
    17401 tttcaaaata gcttgaagaa tataattgtt aataccgtta tagatcaaaa cttcagaacc
    17461 attctctctt ttgatgcgca tcgctaactt ggcagccgcg ggtttacctt tttctggtgc
    17521 actcttttcg tttcctaatt ggacaggaac aatctcattt aatttataca tagaaaacct
    17581 ccagacttct tgatagctca agtatatctt gaggtgcatt tgttagtaag gtactgttct
    17641 atggggcgct tacgttgaaa aacgttgatt tatcggcatt ccccatccat attatattta
    17701 cgtcttattc ccactcaata tgagtagcct cgtaaagaag ctattatatc agtgcttttt
    17761 cagcctattc ctcattttgg tagggaaatg gtagggttag atttttactg cttgatttcc
    17821 attaaagttt gcaactttag attttgttgc acaggtaaat ttaatgtttc ctgtcatttg
    17881 ttctgcaatt ccttcatcat tacggctcca tagatgagcg tagtgcttta aagtgatttc
    17941 agggctagaa tggcctaacc gttgcgacac gaccagaatg tctgcgttaa actcattgat
    18001 taggtagcta acatgagaat gacgtaatcc ttttccttgt attaggggta ccttagctag
    18061 tttggcataa cgctgaataa ttcgttgaac agttgaacga tagagtggga ggtcagtgta
    18121 gctaagaata aattgttcta caccatgatt tttttgaata actttccact tttttagcac
    18181 ttcaattgta tcatcgtcca gagaaatgat ccgcaagccg ctttcagttt ttgtgtaagg
    18241 tttccgaata aaatcttttt gatttgtcat ttccaaggtg tgatgaaccc tcaacttttt
    18301 acgtttgaag tccacgtcat tccaattaag tgctaatcct tcgcttacac gaacaccagt
    18361 catgtaatat agccagatca ttaaaaaaca catatgttcg tagaaatcat ctttataaat
    18421 ttttgccaaa acttcttcaa attcttcttt tgtccagtaa ggaacgattg attttccttt
    18481 tggaattgct tttgttcttt tggaaatatt ctcattcaga aattgcaggg tgaccgcata
    18541 gtctaatgat ttacgaaaca taccgtacat aagtgaactg tatgcttgtg agtatccgct
    18601 atcgttcaat aaccaaattc gataattttc acaatctcgc acattgattt cacgaagctt
    18661 cttatcaccg aaaaattcag taatctgaat caatccacac tcacgagaac tccaagtact
    18721 tttttgtacg ctagctttgt agtgtggaac gtaagactgc tccataaatt ctttgtaagt
    18781 taaatcataa ttcgcaaatc catttacaac catgtattca ttttttatac gcatgacttc
    18841 tttgtgtgct tcacgagctg atgagaatcg ttttcctaga gaatcttttg ttcctttctt
    18901 ctggatcctc tttccagtca cttgatctgt tcctaactca actagataag aaaagcgacc
    18961 atttttatct tgatacacac aagggtattt tgttttcata gttattcctc ctgtgaaaga
    19021 ggaacaccta taatttcttc aattacttgt cggggtacta ccccaactct tttattatta
    19081 taatatggat atccctgatt taccattctt gtttttgctt gtcggattat tgtggtagct
    19141 tgatgtgttt taaatcctag agcctctaaa tcttttcgac aaattgtatt tttcattgta
    19201 taaactcact ttcaaagaaa acactatcat tgggtaatag tgttatttct ttatttttgt
    19261 ataatgaacg gtactttctt gaaaatccat cacttgttca ggacttccca actcatttaa
    19321 agctaaagca ttgtaaaaaa atggacgtct aactatatat tcgcttgtat atagtatatc
    19381 ttgattcgtg gaatcatagg gtttctttac catctttttg gtgacagttg gtagtttgag
    19441 attccgggac ttgaaaaaag cttttttctt atgctctttc aattccagct ctttatcgaa
    19501 atatttgctg atatagcgcc cgcgattttc gatactgtct acatcgatct tgttgatctt
    19561 aacgaaaccc tgaccccaaa tcgtagttaa ccggttaaat gagataaagg ggaaatcaaa
    19621 caagataata tggtagtgga ttgcaccgcg ttgctgcttt tcccacgtgg cgagatactt
    19681 taaatttgct tttttagttt tatagaggtc ataattcagt cgtttgataa attttttaaa
    19741 ttcattattt gcatactcaa tgtcttgaat gttttctttg aaagtgagag ttaaaaattt
    19801 cgtttgattg tcaaaattcg tgtctacaag gcgggcaatc tcaaatcgca tactttgata
    19861 atgtttctgt ttgcgtttta aactgtcata ttgtccttga gcggataatt catcatagtt
    19921 ttttcttttt tcaagagtaa tatcattttc ataaatccaa gctaattttt gttttcgttc
    19981 cagatttact tcgttttcga tcgttttacg tttgggagta atgattgctt cataatcata
    20041 aatttcaata tacgtgggag tttcaatgat tttctgatta taagcttgca aaaatcatca
    20101 ctccttgttg cgtattcgat tgtcaaagaa ccagtcattt tctcctgatg ttgatctata
    20161 aatcaagtta agggggaagg cctgcgtagc agcccttcta gcctatgggg tgttgggcgg
    20221 tcttaacgga cagaaacggc tctgtgggcg ctgccccgcc gtttcgtcct aaccacccaa
    20281 cactccatag tatagttagt tttgtgtagt ggtaccttgt tttctgggaa tcaaagttgc
    20341 cgtatcagcg gttgccgata acccataact gacaaattgt ccattcgagc gtccccaagg
    20401 tacaatcacc aaattggtaa atgtaattat ggctaaacct tgttgggtaa gttcttccgg
    20461 cgtgatcaca ggatgttcag atttgacttt gacactgatt ttttcgtagc gaagttttgg
    20521 taggatacat tcgtagctcc aacctagaat ttcgccctcc ttttgccagc gagtgacatt
    20581 gacgacttgg tattcttttt cgagtgtttc ttttggtaaa ctgagatcgg taatttttat
    20641 tggcatcaca attctccttt tttatacgaa agtttatacg atttgattaa aaaatataga
    20701 tggccatcta cgacttgtat tatatacgag aacttatacg tagtcaagag attttctaaa
    20761 aaaatatata cgatttttta tacgagtgtt ataatgaaaa aaatgataag gaggaatttg
    20821 aatatgtttg gtttagtaca acctaataaa gtgaaggtgg gccaacgaat caaagaaata
    20881 aaagacggaa tgtctctctc ttttacagaa ttagggaatc gattaggttt aaaaaagccg
    20941 acgatcagtt cctacgttca aggctatgcg ttggctccta agagtgtgat caaccaatta
    21001 tctagtatca gcggaaaacc tgtcggctgg ttttattttg ggcatgtgga agaatatatc
    21061 cgagagtatt tgtttctcat tggtcagaaa aaaatagtgg acgattatcc tgaaatcatt
    21121 gagaaaataa agcaagaatt ttatacgggc gagttcaaaa atcctgcttg ggagaatgag
    21181 ctaggctatc ctttggagga atttatccaa gaatgttttg tagaatattc gagtgaaatc
    21241 atgaaggact atgtcgtaca gattacgaaa gaggtacttg aagaatccaa aacgctaaac
    21301 aaactatcat caaaagcaaa agaagaagct gtcacgattc tttcatctgg gatcattgac
    21361 tatatcgaga tgagtggtga gatcaagtat ggagagaaag atcgcattgc gaaaatggtg
    21421 aaagaatcaa taaaaacctt tgatgtgaaa caagacctga catttaatga ttcctatttg
    21481 attggaaagt tgatcaatat tcttggtaca gatgaagata cggagctgtt tctttcttct
    21541 ttatcaatga tatttactga taagcatttt tccactcgat ttggtgggga agaattagtc
    21601 gaaatatttc aatcgatgcg accagcgtta atcaaactct atcgggagaa aacaccagat
    21661 gaattttatg attggtttga gaagtaaaat aagaaaagtg gcttagatta caagttggtg
    21721 aaaaaatttt tttcagcata acatcagtaa tctataaagg ggttgttgat tatactgtag
    21781 aagttaagta ctcaagagat tttttgtacg taaacaaagc aaaaaagggt gcagtttcac
    21841 tagaaaatgt actctttttt gtgatttttc ttagttatag agtttttttg aaactttaaa
    21901 aatgagaaaa catattgatt ttaaagagtg tagacatagc ttcttttgct aaatagatta
    21961 aacttactgg tgagtggtct tgaacatact tttaaatcac attttactga gttttattgg
    22021 attttata
//
LOCUS       DAYYCW010000048        21813 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000048 DAYYCW010000000
VERSION     DAYYCW010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.048"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACHAYA_001577"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACHAYA_001577"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM8605377.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACHAYA_001578"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACHAYA_001578"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM8605378.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACHAYA_001579"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACHAYA_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM8605379.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACHAYA_001580"
     CDS             complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACHAYA_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM8605380.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACHAYA_001581"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACHAYA_001581"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM8605381.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACHAYA_001582"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACHAYA_001582"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM8605382.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="ACHAYA_001583"
     CDS             complement(8557..9027)
                     /locus_tag="ACHAYA_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM8605383.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="ACHAYA_001584"
     CDS             complement(9073..9498)
                     /locus_tag="ACHAYA_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM8605384.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="ACHAYA_001585"
     CDS             complement(9565..9834)
                     /locus_tag="ACHAYA_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM8605385.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="ACHAYA_001586"
     CDS             complement(9845..11440)
                     /locus_tag="ACHAYA_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8605386.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ACHAYA_001587"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ACHAYA_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM8605387.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /gene="pth"
                     /locus_tag="ACHAYA_001588"
     CDS             complement(14993..15553)
                     /gene="pth"
                     /locus_tag="ACHAYA_001588"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM8605388.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="ACHAYA_001589"
     CDS             15906..16880
                     /locus_tag="ACHAYA_001589"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8605389.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="ACHAYA_001590"
     CDS             17050..18450
                     /locus_tag="ACHAYA_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM8605390.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="ACHAYA_001591"
     CDS             complement(18528..19250)
                     /locus_tag="ACHAYA_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8605391.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="ACHAYA_001592"
     CDS             complement(19329..19835)
                     /locus_tag="ACHAYA_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8605392.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="ACHAYA_001593"
     CDS             19958..21757
                     /locus_tag="ACHAYA_001593"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM8605393.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6054 a         4752 c         3910 g         7097 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtaaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggttga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctggaaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ttgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaataaaaa
    21781 aagagttagt ccagaatggg ctaactcttt ttg
//
LOCUS       DAYYCW010000049        20826 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000049 DAYYCW010000000
VERSION     DAYYCW010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.049"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACHAYA_001594"
     CDS             591..1280
                     /locus_tag="ACHAYA_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8605394.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACHAYA_001595"
     CDS             1267..2475
                     /locus_tag="ACHAYA_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8605395.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACHAYA_001596"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACHAYA_001596"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM8605396.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHAYA_001597"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHAYA_001597"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM8605397.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACHAYA_001598"
     CDS             complement(6578..7273)
                     /locus_tag="ACHAYA_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM8605398.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHAYA_001599"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHAYA_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM8605399.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACHAYA_001600"
     CDS             complement(8472..8663)
                     /locus_tag="ACHAYA_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM8605400.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACHAYA_001601"
     CDS             complement(8684..9574)
                     /locus_tag="ACHAYA_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM8605401.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACHAYA_001602"
     CDS             complement(9561..10328)
                     /locus_tag="ACHAYA_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8605402.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHAYA_001603"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHAYA_001603"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM8605403.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHAYA_001604"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHAYA_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM8605404.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHAYA_001605"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHAYA_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM8605405.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACHAYA_001606"
     CDS             14899..15282
                     /locus_tag="ACHAYA_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605406.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHAYA_001607"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHAYA_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM8605407.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACHAYA_001608"
     CDS             complement(16226..17044)
                     /locus_tag="ACHAYA_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM8605408.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHAYA_001609"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHAYA_001609"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8605409.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHAYA_001610"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHAYA_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM8605410.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ACHAYA_001611"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ACHAYA_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM8605411.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..>20826
                     /gene="dnaN"
                     /locus_tag="ACHAYA_001612"
     CDS             19704..>20826
                     /gene="dnaN"
                     /locus_tag="ACHAYA_001612"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM8605412.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVR"
BASE COUNT         5939 a         4249 c         3825 g         6813 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgta
//
LOCUS       DAYYCW010000050        20660 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000050 DAYYCW010000000
VERSION     DAYYCW010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.050"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..1239
                     /locus_tag="ACHAYA_001613"
     CDS             37..1239
                     /locus_tag="ACHAYA_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8605413.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
     gene            complement(1416..2684)
                     /locus_tag="ACHAYA_001614"
     CDS             complement(1416..2684)
                     /locus_tag="ACHAYA_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM8605414.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     regulatory      complement(2735..2802)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(2845..3492)
                     /locus_tag="ACHAYA_001615"
     CDS             complement(2845..3492)
                     /locus_tag="ACHAYA_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFM8605415.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     gene            complement(3757..4260)
                     /locus_tag="ACHAYA_001616"
     CDS             complement(3757..4260)
                     /locus_tag="ACHAYA_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8605416.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            complement(4384..5148)
                     /locus_tag="ACHAYA_001617"
     CDS             complement(4384..5148)
                     /locus_tag="ACHAYA_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM8605417.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            5256..5531
                     /locus_tag="ACHAYA_001618"
     CDS             5256..5531
                     /locus_tag="ACHAYA_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM8605418.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            complement(5879..7030)
                     /locus_tag="ACHAYA_001619"
     CDS             complement(5879..7030)
                     /locus_tag="ACHAYA_001619"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8605419.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(7050..7772)
                     /locus_tag="ACHAYA_001620"
     CDS             complement(7050..7772)
                     /locus_tag="ACHAYA_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8605420.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            complement(7765..9216)
                     /locus_tag="ACHAYA_001621"
     CDS             complement(7765..9216)
                     /locus_tag="ACHAYA_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8605421.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            9358..10440
                     /locus_tag="ACHAYA_001622"
     CDS             9358..10440
                     /locus_tag="ACHAYA_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8605422.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLPEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
     gene            10774..11712
                     /gene="yidC"
                     /locus_tag="ACHAYA_001623"
     CDS             10774..11712
                     /gene="yidC"
                     /locus_tag="ACHAYA_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM8605423.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(11893..12297)
                     /locus_tag="ACHAYA_001624"
     CDS             complement(11893..12297)
                     /locus_tag="ACHAYA_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8605424.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(12313..13122)
                     /locus_tag="ACHAYA_001625"
     CDS             complement(12313..13122)
                     /locus_tag="ACHAYA_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8605425.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(13109..13999)
                     /locus_tag="ACHAYA_001626"
     CDS             complement(13109..13999)
                     /locus_tag="ACHAYA_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8605426.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            complement(14012..14491)
                     /locus_tag="ACHAYA_001627"
     CDS             complement(14012..14491)
                     /locus_tag="ACHAYA_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8605427.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            complement(14508..16274)
                     /locus_tag="ACHAYA_001628"
     CDS             complement(14508..16274)
                     /locus_tag="ACHAYA_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFM8605428.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFTTGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            complement(16320..17261)
                     /locus_tag="ACHAYA_001629"
     CDS             complement(16320..17261)
                     /locus_tag="ACHAYA_001629"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFM8605429.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            complement(17258..18253)
                     /gene="lacD"
                     /locus_tag="ACHAYA_001630"
     CDS             complement(17258..18253)
                     /gene="lacD"
                     /locus_tag="ACHAYA_001630"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8605430.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            complement(18246..19439)
                     /locus_tag="ACHAYA_001631"
     CDS             complement(18246..19439)
                     /locus_tag="ACHAYA_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8605431.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            complement(19457..20185)
                     /locus_tag="ACHAYA_001632"
     CDS             complement(19457..20185)
                     /locus_tag="ACHAYA_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8605432.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
BASE COUNT         5996 a         4450 c         3618 g         6596 t
ORIGIN      
        1 taagaaaaaa tttacaaatc gaaaggagtt cattcaatga atcttactag agacctgata
       61 tttttagtta tctatatttt aggaatgtca ggcatcctct attggcgcaa gcgccaaagc
      121 gatcccgctc tattctttta ttgtgggacg tggactttta tgatgatggt cacttacttc
      181 gtcgttttag aagtttttta tcatacattt tattttttga ttccattgct atttttcagt
      241 atttttgcat ttttctattt taaggaaaaa cgtaggttaa tcaatggcgt actttttaat
      301 acgtttttga ttgtctttgg tggttatcta gggtggctac taatagagac gcaaaatatc
      361 tttatcggtg ggttattggc tttgatcggg ataccaatat taattctttt ggcaatcggt
      421 atctacattt tgatcatttt tttattttgg aatggtgttg tagtcatgaa aaaagaatcc
      481 cgttctttgg ccaacctttt gactctcttg ttaggagtcg ctttgactat ctttttgata
      541 ttggactata cattagtcca ttggctccca caatgggcaa atgctctatt tgctgtcatt
      601 ccgtttattt ttgcttatct actcatcgtt ttctttaatt ttatgactgt ttcaattttg
      661 tatcagttca accgtccaag gtataaccaa gattatatcg ttgttctcgg cgctggcttg
      721 attgatggtg aacgcgtatc tcccctactt gctaaaagga tcaataaggc gatcgcattt
      781 tattgggcac aaagtaaagc aactttgaat ccgccagtct tgctcatgtc aggaggaaaa
      841 ggaaccgatg aaaaagtagc tgaagccatt gcaatgaaac aatatgcttt agaaaaaggg
      901 attcccgagc gtgatatttt agtagaaacg aattccacaa cgacgctaga aaatatgctg
      961 ttttcaaaag aaataatgga tcagcaaatg aatgggcctt atcgcgcgat ttttacttca
     1021 aataattacc atatctttag agctggacta tatgcgcgac aagcgaaact caatgcaaac
     1081 ggaatcggtg caaaaactgc actatactac ttaccgaatg ctatcttaag agaatttatt
     1141 gccatccttg tcttacacaa aaaacgccat cttatcatct gtggcttaat gattggcttt
     1201 gcagtatttt ctgctcttat tctcaaattt gctcaataaa aaagagcgtg ggacaaaact
     1261 aaaaatcagt tttgccacag actctaactc cgaataaacg gtgggagcag aagcaactcc
     1321 ttcggaaata agcgaaattc acaaaaattt aaaaagcaat tttcgtgaat ttcttcttat
     1381 ttctcggagt taaacacttc tgtcccaacc tctttttatt tctctttttg tcgtgagatt
     1441 cttccaagga tcggtaggaa caagattcct tttttcactt caactttttg cgcagaatag
     1501 attccactat ttcctgaaca cataaagcta atcaaacaga tcataaagaa gtagctggct
     1561 gcttgacctc caaaaagttc gatccccata acaaagcaag caatcggtgt attcgttgct
     1621 ccagagaaca ccccaataaa ccctaatcct gctaaaaaag gtactgatac atgaagaatg
     1681 ggtgctaatg tcgcacctaa agttgcccca atctcaaaca aaggggtcac ttcgcctcct
     1741 tggtatcccg cgccaagtga tagaacggta aagccaagtt tcccaagaaa atcataagga
     1801 tgagcacttc cattaaatgc atcttgtaat aaaggtaagc ttaatccgag atatctttgc
     1861 gtctgtaaaa tcactactac gagtaccacg actgctcctc caataaagtt gcgtaaaact
     1921 ggatggggga tccgctttgc ataccaagct tttaaccaga cgatcgaacg gctaaagagc
     1981 cagccaatga taccaaatgc aatgcccgca agcaacaatt tgaagaaaag tgaagggtcc
     2041 catgcaggaa tcgcccccat ttgataatgg gtatgcttga cattgaaact ttccgttaca
     2101 aagttcgcaa aaagtgctgc aaaaaagctt ggaaacagtg cttcggttct cacttttccg
     2161 attgccaata cttctaatcc aaacaatgtc ccagccaagg gtgtcccaaa tactgagcta
     2221 aaacctgcgc tgatcccact gatgacaaga atctcacgct cgattttgtt caattttagc
     2281 caacgagcaa aattctcagc aagtgccccg cccatttgga ccgctgtacc ctcccgacca
     2341 acagaaccac caaaaagatg cgtggtaatg gtgccaaata aggtcagtgg gatcagccgt
     2401 aatggaatct tttcatttcc tccattccct tgatcgatca ctaaattgtt tccccgggaa
     2461 gaattgcccc catacgtcgt atatagataa gcaaaaagtc ccccacttat cggcaaaaga
     2521 aaaagtaacc aaggatgctc cagccgaata gctgtcacat cagtcaaact agttagaaag
     2581 aatgcggata aacttcccat aaaaatacct aaaattgctg tgatcaataa ccatttgatc
     2641 atatagatga tcaccttcaa tggatcaggt aatcgatttt ccatctcatt tcctcctcaa
     2701 aaaaacgcct actaaaaaaa ggggtagccc cgtttcttta gtaggcgtca ttagttatct
     2761 tacgatcaac gtttaaaggc gaacaccatc acctattcac ttcaccaaat aagatagcat
     2821 atttttcaga aaaatacaat gaaatcaccg ctcacgtttc actcgttctg tgaattcgta
     2881 aaaaggagct aattgtacca actgctgatt acattggtca ctatcatgac aaatataatt
     2941 tccttttttc gtgtatgttc catctgaacc agtttttgtg gtagaaagaa agagtgaaac
     3001 atttgaagtt ctttgacaaa tcgaacaaat tcctttgttg attgtaggag ataatgttcc
     3061 actgatccct tttagttttt cttcttcata atagatcata aatttttttt ggctacctgg
     3121 atcattccaa ccaatataag tatgttccct taaatccagt tgattccaat ctggttgttt
     3181 taactttttc acttttcgga ataatttatc gatctgcggt tttgtcggtt gtatgaatgg
     3241 aagaacgatc actttcaatt cctctaatag tcgttctgct ttttcttttt ttagagtgac
     3301 gtctagtacc ttttccataa attcatttac agctgtatta ggatgaccaa gtacctcgtg
     3361 gatcttttcc gcagctaatg cttggactgt ttggactgtc tttggatcat tcactgaact
     3421 ataggcgctt aacagctgct cgacttctcg cttgataaaa taatattgat atggtttgat
     3481 tgttttttcc atgactttga gtcctttctt ctttcaaaat cggctacttt gaagaaaaaa
     3541 gtgatctttc tatcaaagtg cctttagata aagccctcaa actcttttaa ataagataca
     3601 catcgcatct tatcatcacc ccctttattt ttatgttggt tatgtacttt ttcatctcaa
     3661 caaagtaaac actaaaaaga ccaattagtc aatgtctaaa caaaaaaccc gaggattttt
     3721 ttcaaaatac tcgggtcaaa tacttagtta actaccttaa atcgattgtt ctttaccgaa
     3781 atatcgttgc catttttgtt tcattgaagc tgtcattggt tgagctgctt ctttgactgc
     3841 acaatattta tccacaaaag taacgatata accttcttta tatttaggag gggcaatcgt
     3901 tgctccccac atatgtttca aaataatatc acgctctaga tctgaaagct ccgtgatttt
     3961 ttcagcattt ttcacagcga tccttgggtg aacatacgca tgtgtacctt catcgaactt
     4021 cgtcgtacgc caatcgtagt aaaataaatc atgtaaaagg cctgctcgag cagtcgctct
     4081 tgcatcaccg ccccattttt tcgctaattt gtagctatag taagacacac tgattgaatg
     4141 ctctaatcgt gtcgagtgga catgttgcgt gtaatttgct aatttcttta ctgcatcagt
     4201 ttctaataga tcatttatat aagacatata ttcttcgtct tcacgccatt tttctgtcat
     4261 aattatcgat ctgccacctt tttgaattcc atttatcttt gaaattgaac ttagtatacc
     4321 actagtgttc gtaatattct agtgaggttt tcgcaaaatg cagaaagctt aatagttcct
     4381 taactatacg gagaagtgat aaagcatgat gcccgctgca actcctacat ttaaagactc
     4441 cgcatttcct tgaatcggta tatacacatt tttatccgtt agcgccagca cttctggacg
     4501 tacgccttga ccttcattcc ccatcacgat cgcataattt tctgttgtct ggatatcatt
     4561 cagggacact gcttcttcat tcagttctgt tccataggtt ataaatcctt gctctttaag
     4621 gataggcaat agttttgtta gatcctcttg gatgatcggt aaatgatagt tactcccctg
     4681 catgcttctt aatactttgg tactgtagac atccgccgtt cccttaccta gtacaacaca
     4741 agatagacca aaagcatcgg ctgtgcggat cattgtccct acattgccgg gatcttgtac
     4801 gttgtcgagc aacaaccaac caccttcaaa attcggtatc tctctttttt cagcttccac
     4861 cactgccatg atcccttgcg gagttggtag atctgaaaca gttgccatca cttcttctga
     4921 tactagtgta acagaaagcg cctgattttc ttcaagccaa ctagcccact cagtcagacc
     4981 gcgttgcgta ataaaaagtt cgttgacctt cgcatttgag gcaacagcct cctcgatcag
     5041 gtgaaaccct tcaagcagat attccccttt ttcttcacga tattttttct tatgtaattt
     5101 ttttaattct ttgattcttg gattttttgc tgattggatc tctttcacat ttttcacctc
     5161 aactgattat accatggatt agatcattgc acttgttcag tagcaaaaaa gtcggtatca
     5221 ttaatttaaa aataaaagaa agaacggtga ataaaatgaa caaaatcaag atgaacgtgc
     5281 aaggacgtgt acaaagcgtt ggttttcgct atatgacaaa aatcgtagcg gatcaattag
     5341 ggatcaccgg aacagtgaaa aatgaagatg acggctctgt cacgatcgaa gctgcgggcg
     5401 acgatgaggc aatcaaaaaa ttcgtggaag aagtcaaacg atcaccaagc cccagcggac
     5461 gtgtccaata tgtagacctt caggaaaatc cgatgatgga ggacagaaaa aagtttgttg
     5521 taataggctg accttctaga aatgaatagg aaatgtcaga aaaatcggac tgtctgtatg
     5581 tagatcgaaa aagtttgccc ctctctccat cctagatcga caaaaaaaca ttcttattag
     5641 cgagtttagt aaccagtgat aggcatacgt aaaagcggga ggacttcaaa aattttggtt
     5701 caataaaatg gagtctataa tattttatgg tagtggaaaa tcgactttga ttttccacta
     5761 ccactttttc attgtcaaat cctgacgctg cccagtcaac aagccaactc tcgaaaggat
     5821 cattttccct ctttgttcta atggataccc atagctgttt tcaaatatac agttttatct
     5881 aaaaatttag ttttgctaaa cgatcaaagg cttcacctaa gatttccaat ggctgagtcg
     5941 ctgctagtcg aatatagttg tccccgatcg tttccccaaa cgcttttcca gggacaaaaa
     6001 gtacgccgct tttttctaaa acctcttcca caaacgttac tgaatcgagc ccactcttac
     6061 tgatattaat aaaagcatac atgcttccag aaacagaaga taaagataag taagggattt
     6121 ctgccactct tttctcaata taagctaatc gttctttgaa tacttcaaca actggcggta
     6181 caagctgttc atagtgcttt aaggcgtaga ttgcacctct ttgagaagca gtcggtgcgg
     6241 aataagtgat gttttcattg atcaacccag caatttgatt gatgtatgct ggtgcgatca
     6301 tgtaccctaa gcgccagcca gtcatcgcaa accccttcga tacaccactg acagtgatcg
     6361 tattatcggg cgcaaatcgc gccatcggta cgaacttctc tttaaaggca aagtcttcat
     6421 aaatctcatc ggaaatgata tagagttcat tttcgattgc gacttcagcg atttttttca
     6481 acgtgctttc agaaaaaaca gctcccgtag gattattcgg cgaattgatg acgatcacct
     6541 ttgtacgatc cgtaatcgct gcttttaaat cttccacatc caactggaag cccttttctt
     6601 cataggtagg aacaaaaaca ggcttgcctc cagataaaat gacttgttcc cgatatggtg
     6661 aaaaataagg ttcatggatg atgacctcat cctctggatc tagtaatgcc tgtaaaacta
     6721 gatacatccc gtgcattgct ccgactgtag ctcgaacttg gttgcattca acgctcaatc
     6781 catatttctt ttgataaaaa tctacgaccg ttgacaagaa ttctttgctg ccatcaggcg
     6841 cagtataatt agtagcacca ttttttacat ctgtataagc tgcttcaata atcgtcggat
     6901 cagtgatcaa gtcaggatca ccaatcgata aatcgataat atcctctttt tcctttgcca
     6961 aaatagcaat attcatcaac agattttctg ctggttgctg aaaccggcgt gcaatcttac
     7021 ttttgtccat cttacttcct ccatctttgt tacaacactt tgttcaagaa atcctgtgtt
     7081 cgactatttt gcggattttc aaaaatctct ttcggtgttc cctcttcttg aatgatgccg
     7141 tcagccataa aaatcacacg atcggcaact tctttagcaa aacccatttc atgagtgacc
     7201 acgaccatcg tcatcccttt tttagctaaa tcctgcatga ctgccaacac ttcaccgacc
     7261 atttcaggat caagtgcaga tgtaggttca tcgaaaagca tcacatcagg attcattgct
     7321 aatgctcgag caattgctac ccgttgttgc tgtcccccag ataaactggt aggataactg
     7381 tccacttttt cctttagacc aacttggttc aataatgata gagcatgttc ttttgctttt
     7441 ttagggtctt cttttttgat ttttacaggt gtgatggtca aattttcgag tacattcata
     7501 tggggaaaca aattaaaact ttgaaatacc attcccattt tttgacgaat ttgattgata
     7561 tcgctgtcag gggctgtaat gttttgtccc ttaaaaaaga tttcaccaga agttggttct
     7621 tccagcagat tcatacaacg tagaattgta cttttcccac ttcctgaagg gccaatgatc
     7681 accactactt ctccttcttt cacagaaaga tcaattgatt ttaagacttc attctcgcca
     7741 aaactttttg acagtttttt tacattaagc attcagaccc attctccttt caactaaagc
     7801 taatatacgg gaaatactga aggtcaacac gaaataaatc aaagagacca acacatatgg
     7861 taggaatggt ttgaagctag caccttgtac cacgcccgtt tgaaagatca attccgatac
     7921 cccaatgacc gaaacaactg aggactcttt gatgatcgtg acaaactcat ttcctagagc
     7981 tggaagaata tttttgattg cttgtgggaa gatgatgaaa cgcatggctt ctttctggtc
     8041 cattcctaaa gaacgtgcag cttccatctg tcctttgttg accgcattga tcccggcacg
     8101 gacaatttcg gctacataag cagcactatt caacgagaca gcaatacaac ctgctaccag
     8161 tttcgataga tccaatccca gtactcctgt tccgaagtaa acgataaaga tttgaactaa
     8221 gagaggtgtt ccccgtacgt attcgatata aataatggct aatgccttta aaaatttatt
     8281 cttacccaat ttcatcagtg caagcaaact accaaagacc attccaaaca atacacccac
     8341 aaaagctaat aaaatagtgt attttgttcc aatcaaataa tacggaccgt atttttgaag
     8401 aaaggactga tcatcttgga acatcaattg attcgcttgt ttttgataat ctttcagcaa
     8461 gccgtcgtca ttgatcgttt tgattgacgc attgacatga tttttcagtt ctggtgtatt
     8521 ttttgacatc gcaatagctg tggttttttc gccttgcgca aattttactg gagtcaaagc
     8581 tactttctta ttctgttggg catatgcttc tgcaactggt tgttccagca caacaccttc
     8641 aactttttca ttcagcaaat tattgatcag atcaggaact ttttgcaggg aggtcaactc
     8701 tgcaccagtc agttcatttt tcactaactc ctcttgtgtg gtttgctttt gcgccccaac
     8761 ggctaagcca gaaaagtcgg ctgtcgtctt aaatttattt ttgtcctctt tacgaacgag
     8821 tactttttgc tgtaccacga aatatgggtc agaaaagtca acttcttgta aacgttcttc
     8881 agtcaccgcc ataccagata taactaaatc gatcttgcca gttttcaacg cacctaataa
     8941 tgcatcaaaa ccaagttctt ctacttttag tttcacaccc atatcctttg cgatcttttc
     9001 ggcaatggat acatcaaaac caacgatttt atccttacca tccacattcg catggaattc
     9061 atagggcgca tagtctgcag ataggccaac gactaattct ccactttgtt ttatccgctc
     9121 gtaaatttca tctttttctg caccttgtac ttttactggt aaaaaaacca gtagaaacat
     9181 cagcgccact aataatatgt ttattccttt tttcatccat ttgaccacct ttgaataata
     9241 atgtaatcag aaaagctata aaagaataaa tattcataaa taacttgcta atatactagc
     9301 ataactatta taataaaaaa agattaattt aaaaaaataa tggaagaagt tttttatatg
     9361 acaaaaaatt taattgttcg cggtgcccct caagagtatg aaataaaaga aaacgcatgg
     9421 gattcactcc ctgaacacct ttacaaaagg catattgaca acgttttgat tctccatgga
     9481 aaaacatctt ggaaagcagt aaaagatgtt tttccagatt tatcaataac aaaaattttt
     9541 gaatattatg gtggagaatg taccgatcca cttgctgaca gttttgtttc tttatgcaaa
     9601 aagaaggatt tcaatggtat tattgcagtc ggcggcggga aagtagcaga tctaggaaaa
     9661 ttgattgcct ataagacaaa cttaccaatc atcatcttac ctaccttagc tgcgacgtgc
     9721 gcagcttata ctccacttag tgtggtctat aatgaagaag gagtgatgct tcgctatgat
     9781 atgttcccta aagcaaatga tttagtgttg atcgatccaa agatcatttt gcactcccct
     9841 cgcgagatga tgattgccgg tattggtgac accttagcaa aatggtatga atctcacgca
     9901 atcatctctc agttaactat caagcctatc gaagtccaag tagcggaatt tgcagcgtta
     9961 cgatgccgcg aaatcctttt agagaaaagt gcagaggcaa tggaagcgat ggaccagcaa
    10021 aaaatcaatc aagcttttat cgatgtcgtg gaaacaaaca ttttattggc tggtatggtt
    10081 ggtgggtttg gcgatgacta cggccgaaca gctggagctc attccattca cgatgcgatg
    10141 accgtactcc ctgatagcca tcagcaactt catggaaaca aagttgcata cggtatcctt
    10201 gtccagctga tgatcgaaaa taaattagca gaaatcgaac acctactgcc gttttatcat
    10261 cacttgaact taccaacgag tttagctgat atgggtttac atttaacaga aagtgactat
    10321 caagccgtag ctgaacgtgc atgtttacct gatgaaatga tccacttcat gaaagaaacg
    10381 atcacaccag cactggttgt ccagaaaatg aaagagttag aacaagtgac acagggatag
    10441 ttagcccctt ataccatggc agtcagcttt tttatggatc ggctcaatgg taaaaaaaaa
    10501 gaagtggaaa aaattgacat tccacttctt ttttttgcgc taaaattatg atttcaagga
    10561 aatatccgtt gctcatctta ttttagagaa cggaggtgct aagtatgctt tatactgaag
    10621 tatttttagc tggattcttc tttttaacat taagcgtctt ttttgatcct ccaatcggtt
    10681 atttctatga aatttctatg aagttgattg aattatgagc gcttttttag tatagttaag
    10741 cttgtgtaaa aattataaaa gaggaagtat tttatgaata aatggaaaaa ttggttattg
    10801 ggctctgggc ttgtcacgat catgcttttc ttgtccggtt gtgtccgcat ggattcaaat
    10861 ggtaaccctg acacttcagg aatcatttac cgtatattgg ttcacccaat gggacaagcg
    10921 attacatatt tagtggaaaa ctttaattgg tcctacggct gggctgttat cttgatgaca
    10981 gtcatcgttc gtatcatcat tttgccatta ggtattagtc aatcgaaaaa aacaatgatc
    11041 caatctgaaa agatgcaagc attgaaacca caagttgaag ctacgcaaca aaaattgaaa
    11101 gctgcgacaa ctcgagaaga acaaatggct gcacaagctg aaatgcaaca agtttatcgt
    11161 gaaaatggct taagtatgac tggtggtatc ggttgtttac cgcttctgat ccaaatgccg
    11221 atcttttctg cgctatattt cactgctcgc tatacagaag gcatccgtga atcaaccttt
    11281 tacgggatcg acctaggtaa ccctagctta gttttagtag cgattgccgg tattgcttat
    11341 ttacttcaag gctatatctc aactatcggt atccctgaag aacaaaagaa aacgatgcgt
    11401 acgatgttga tcgtatcacc cgctatgatc gtcttcatgt cgatcagtgc cccagctggt
    11461 gtcactcttt actgggtagt cggtgggatc tttagttgtc tacaaacatt cattacgaat
    11521 gtcatcatga aaccaagatt aaaagcgcaa gttgcggaag aattaaaaca aaatccgcca
    11581 aaacaagtgg tgacgcctcg taaagatgtc acacctgctg aacctgcgaa gcaaacaaaa
    11641 aaacaagtac aagcaccaaa aaacaatgcg aagaatggtc ggaatgctgg taaacagcaa
    11701 aagcgtaaat agttatttgt aactagcttc ttcgatcgac ccagatcatt tctgatttta
    11761 tgacgataaa agccaacgta ctgtgaaata tagaaaacta tatttcgcgg tacgttggct
    11821 tttttataca cggcctcttt atacatccgt atttatatcc agttgtatca ccagtcaaga
    11881 agttgtgctt gattattctt cttcatcgtc tcccatgcca gcaatcactt ggttcactga
    11941 aacgatactc tcacgacccg aaccaataaa atctggttct gctccaatca tttgcgaatt
    12001 gatgaattcg atgaccatcg gcaaattcat gccagcatat agttcgatcg tttcaccttc
    12061 catgattagc ttggaaacgg tatttgcagg tgtcccaccc aacagatcgc aaagcactac
    12121 gtaatctttc aatggtgctg taacggagag gaatctttct ttatacgtgt ccgcgccatc
    12181 ttcaggcaat aaagggatcg tatagatatt ttcttgtggt cccatgatca tttctgtact
    12241 gttttttagt tcttcacaaa atttaccatg actaatcaag actagttgtc tactcatctt
    12301 tccaattctc ccttatttcg aaatcacacc taatgcagat aatgctacac atacgatcaa
    12361 tacgatgaat attgctcgag taggtgtcat atttttctta cctaacatcc aataaacgcc
    12421 accaacgatt ccagctggta aaagtttagg taagatcata tcgatatagt tttgcatatt
    12481 caacgttaca tccccaatcg ttggtgccca tgagaattta acattgatca tggttgctac
    12541 taacgctcca accatgaaaa ctcctagtaa agtagccgca tctgtcaatg cagtcaagcg
    12601 tgattgcatc gtggtaacta gagaaatccc ttctttgtag gcaaattcta gttgtttcca
    12661 acgaaagacc atgacagcga cctgagcaag gatccagata aggataccta ctggattacc
    12721 atctgttgcc atatttgcgg ctaaagctcc aaatatggtc gggatcaatg ccgcaaatac
    12781 agaatcacca attgcagcga aggaacccat caatcctgct ttcatcccag aaacagtttc
    12841 ctttgaagag actccttctt tttcttcgat agcaagatcg atcccagtaa tgatcgtgtt
    12901 aaagaaatta gacgtattaa aaaattgcgt atgtgttttc atcatttctt ttaattctgg
    12961 tgtatcatca ccatagattt ttctaagttg tggcaagata gtatacagat aacctgaacc
    13021 ttgcattctt tcatagttcc aacctaattg gaaagtaaac aaacttctac gattgatctg
    13081 cttaaaatct tctttggtca atttataatt agatttcgtc atcttcgatt tccccctcat
    13141 cagattgtga ttgctgactt actactggat gttggtcatt cttttgatca ttcttataac
    13201 tgatcgttgc cagtgctaga ccaatcaatg ccacagcaag catcggtaat gaattgaatg
    13261 ttcctgggaa atctttgacc acgctagcca cacttgttcc taacaattgg acattcgaga
    13321 acactgttgc taataacgct gtgatcgtaa atcctaaaat cagataagaa atgtgtttct
    13381 ttactggtag gtagcgtagt aaaattgcaa acccaactgc tggtaagact gcccctgcaa
    13441 cagataagcc atctcctagc cattttaggt cgccgtttaa aacagccaca acattttgga
    13501 ctaagccacc accgaatgct agggccaaga atacaggaac cattctggaa agtgcccaag
    13561 gtaatgcccc catcaagaag ttccgttcaa ttgctttgta attcattgtt tccacatgtt
    13621 gatcgatccg atgtgcaaaa tacgtattcg caaaacgtgc caaaatatct aattggatca
    13681 tcaaacttgc tacaggaacc gcgatggccg caatcgcttg ttctgggttc atccctagag
    13741 aaatggagaa agctgttgct aagatcgttc cagagttggc atcgattttt gatgcaccac
    13801 caaatgtccc tacccctaaa acagtcaact gcatacttcc accaatgatt aaacctgctt
    13861 ttaaatcacc cattactaac ccagcatata atcctgcaaa aactggggcg ctcattgaag
    13921 aataaatctg taattcatct aaaatttgat accctgcata taatgttaat aataatatct
    13981 gccaccattg aatatccatg aatcattcct cctatttgat taaaagactc ataaagtctt
    14041 ctgctttgtc atttggcacc atttgagcga tcaaatgaac gccaagctga tctaatttct
    14101 tgaagacctc tacatccgca tctacgacat tgattgattt tgtgatggaa cgtgtttcat
    14161 ctgtttgtga catattgccg acatttaatt ctttgatcgg tacacctttt tctactagcg
    14221 ctaagatacg atctggtttt ttcgctacga tcagcactcg ttgagaatca tatttacctg
    14281 ctaaaatatt tgcggctgct ttttcaatag gtaaaatact taatttgact ccagctggcg
    14341 ttgccaactt taacccactt ttttcgatcg catttgttgc tacctcatcg tctacgacca
    14401 tgatccgacc aatattcaac tttgtcgtcc aaagatttgc cacttgtcca tgtatcaaac
    14461 gtccatcaat tcttactcca ataattgcca tcatccattc tccttttcta acttttttct
    14521 attggttctt tgacaaaatc tataacttca ctgaatctac cttctgtttc aaagataacg
    14581 atttcatttt gtccttttct aaataaaccc tcgggaacaa acagtgaaag aattggtcct
    14641 acttcccaaa aacgaccaat attcgtgcca ttcacccaaa cgatcccttt cccaaactca
    14701 ctaaggtcca aataagtatc accgatttct gacagttccg cttcaaagcg gtaaaatgct
    14761 ggttgacctg cttcccattt ttttgaaaaa tcgatgtgtt ctgtagtctc taaaggtaaa
    14821 caatactgtg tccattctgt gataaaatga agatcgctca tcactcctgt acgtattcct
    14881 tttctttgcg tatcagctag cagcttgtgt ccatagttga cccgtcccat attttcaaat
    14941 aatagatcaa tttgatggtc ttctttctca agtgtcaatg gaatggtctc accgatctct
    15001 gtttggtatt gaatcgcttg cttttcttca tcgagaaaaa catggacccg atcacgtccg
    15061 tcgatgatcc gtaatttttc attgaccgca tcttttttga tcgttgttcg atacaatagg
    15121 tacccagtat tctgacccaa ttcttccatt gtttgaggat aggtcgagtg gatcggcttg
    15181 ctgatggtat ccagtgttgc gaaaaagctg actttttctt tcaagttgat tccttttttg
    15241 acgattgttt cttttatcca tggttccatt tgctgcattt ctggaaaatt ttctgccatc
    15301 attcgtttca gagcaaaata tttttccgtt ggatttccct gctcatccaa tggtgcatcg
    15361 taatcatagg aagtgacttg cggcaaatca atcgcgcctc tggctgaaca gccattcata
    15421 aacccaaaat tggttccgcc atggaacata tagagattga tactgccgat ctgtaatgct
    15481 tctttgactg catccgctaa ttcttccgga tctcttttaa tgatcggctc tttccaacga
    15541 ttaaaccagc catcccaaaa ttccatacac atcaacggcc attttttacc atgtgcttca
    15601 aaaaactggg ccatatctgc aaaattttct tttgcttttg agccaaaatt ccctgtcgta
    15661 aaaacatcat cgtcaatcaa aatgcctgct cttaatgtgg cttgccaagg tccatcagaa
    15721 gtgaatagcg gtacatcgat tccaccttcc tccaagtatt tttttacttg gcgcaggtat
    15781 gctttctctt caccatacga accgtactca ttttctacct gcaccattaa gacgttgcca
    15841 ccatgagtga ctagatgagg aacgatttta ggtagtagcg tttggtaata ccgtttgaca
    15901 tacttcatga agcgaggatc cgctgaacga atacggcact cctttgtcaa tagccaagca
    15961 ggcaagccac caaattccca ttctgcacaa atataagggg ctggacgtac gattgcgtat
    16021 aatccaaggg attgcgctaa ctctagaaaa tgggaaagat ccaatatttt cgaaaaatca
    16081 taggttcctt cttctttttc atggagattc caaggcacat aggtttccac agtattaaat
    16141 ccaagcgctt tcaaattgtg caatgaccgc tcccaattcg tcggatgcac tcggaaataa
    16201 tggatggcac cagataaaat tttgaaaggt tgaccgtcta acaaaaactc ttcttttatt
    16261 tcaaatctag tcaagcaaat ttcctcctcg tttttagaaa ccgctttcta attttataat
    16321 caacgaatct ttctgacgtt gatttccccc ctaaaataat cgactttcac aggatcgatc
    16381 catcctgttt tttcttgttg tgcattcgcc atcccacagg ccatcgcaaa cgcaagtgtt
    16441 tcttcaatcg tatagctctt tgatagagca tgtgctaagc ctgcaatagt cgaatctcct
    16501 gaccccacag gattcaccac cgcgatttta ggaatcgtaa cctgatagaa gtgtctccct
    16561 actttagcga ttgctccttt tgcgcctagc gaaagaacga tccaattgat tccttcaaaa
    16621 agcggatgat ccaataatga ttggatttca tctaacgaag tagccgaaga aattgtttgg
    16681 tggaccaact ctcctaattc ttccaaatta ggtttgatca aaaatggttt ttccttgcct
    16741 tccaagactt caagcaaggt ttgaccagaa gtatctaaca atactcgtgt tcctttttta
    16801 ttcgttaggg caagtaactc tgcataataa cttttagaaa atccttgagg cagactaccc
    16861 gaaatcgtaa agatatcagt tgtttccatt agtcgtgcaa aatgctcggt aaatttatgt
    16921 tgctcttctt ccgtcacaac aggaccgctt tccaatagtt ctgtttgatt cactccatgt
    16981 aaaatagcaa tcgaatctct cgtttcccct ttgataggta aaaattgatg cgttatccct
    17041 gattgatcga gttgttcttt gatatatgct ccatgctgcc cgccgactaa tccagtagct
    17101 aaaacatcat ctgctaataa atgaaggaca cgagcaacat tcaagccttt tcctccagcc
    17161 gttttgttga ccgttttgac ccgattgact tggtcgatcc gtaattcatc caacgtgtaa
    17221 gcgatgtcaa ttgaaggatt catggtaact gtcgtgatca ttttgctgtc accgaatctt
    17281 cttttacttt gtcaaaccaa ggggtggcgt tttcatttat cactttattc aacgattcga
    17341 tattttgtcg cccttgtgtt ctcaaccact caatcgcacc ttctctacca ttttcaacat
    17401 atgctttgac tccatctttc caagtcgcac gtccgcataa cacaccgttg aatgttgaac
    17461 cagattcatg agcaaagatc aatgtttgtt ggaacaatgc agtcgaaacc cctgcactca
    17521 agaagataaa tggtaacggt gttgcatcac tttgttcctt gaagaatgcc gctgcttctt
    17581 ttttactata cgcaatttca ccatcagaat aaccttctac atagttcata ttgactggca
    17641 cttctacttt caatacatcc actttatatt taggatcaga aaattctttc atcatctcga
    17701 tcactttatg cggtttgatc ttcgcatatt ctaatgaagt gctgtcattg gttttggcat
    17761 catacgaaac caattctaga taaaacggac gcttttcacc tgcacattca ctgcctaaac
    17821 gttcgataaa tacatgtttc aaatgattga tctctgggtc ttcatctaca tcataataaa
    17881 gcaaaaactt gatcgcatcg gctgtttgtt cctttaatct aagcgtagac caatccgcta
    17941 ataaatcagg cagacgtcct ggagttgtcg catcataacc cgttttttca taggctaaaa
    18001 gtagtcctgc ttcctgatgt ctggctttag cagctggcaa tccatactct ggatctagta
    18061 aaatagcaga agcataaggc gttaattctg ctgatactaa ttctttgaat tcagtgatct
    18121 gttgcgccgt tggttctttt cctaacgctt tgatcatctt tttcaaagca ccgcgttgat
    18181 caattgccaa tgcactgatg attccttctt ttgttgataa actcttcata gctgcaattt
    18241 tttcttcatt catccttcgt ttcccctttg ttcacatatt catggataat gacacctttg
    18301 accactcgat tgactgtacc tgtaggcgat ggtgtatctg gtttattcgc tacttttatc
    18361 gacgtatcga gcgcaatgat ttgggctgtc atgatcatag gtaaggcaag atacgcatca
    18421 ggtaggctga tcgaagcttc taacaaaaga cttgtacctg tgaaatcgat gtttcctggt
    18481 tgggcgatcg cgatgacact cgtggtaatt tggtcatctt ttatttcttc tagaatatct
    18541 aagtcataat ttcttgtata cggttggtta ttaacaaaac caataacaag tgtttcgcta
    18601 tttaaaaacg attttggtcc atggcggaag cccatagaag aatcaaagat cgttgccact
    18661 ttaccggctg tcaattccaa tattttcaat tgtgcttctc tcgttgcacc agccaaactt
    18721 cctgaaccaa gataggcgat acgttcaaat gacttaggca ataatttttg gatttcttga
    18781 tctcgtgaaa ttagatcttc tccagttctg atcaattgtt gaagataact ttcttttttc
    18841 tcaacggcag tttggtcgaa tacccataac gcactcaaca acatgcacgt aaaacttcct
    18901 gtcatggcaa atcctgcatc atttgatttt tctggcatca gtaaaagata atttcgttca
    18961 tctgtcgctg cattctttgc taactgacct tctttagcac aagtaatcgt caaatgaaac
    19021 aatgtcttga tttgttcatt ggcaatcttg actgttgcca cactttctgg actatttcca
    19081 ctacgtgcaa atgacaccag taatgtcggt tcatccggaa atagaaattc ttttggtgaa
    19141 gacacgatat ctgtcgtacc aaagctatga aaaataaagt gatcgttatc accgtattca
    19201 tttaaataag gtactgctgt atccccgaca tattgtgatg tgcctgcacc agtaaaaatc
    19261 actcgaactc ttttgccatt tgctgctttt tttatctggt caaaaaacaa tgctaatttt
    19321 tcttgattct gtgctaatat ctcagctgtt tctttccata attgcggttg ctgtttgatt
    19381 tctcgcgtag ttatttccgc acccaactct tttaatttat ccttcgatag ttcaaacatg
    19441 ttgttttccc tctcaatcat tttcggacgt ggcggatctt ataatgaaac tggtctgctc
    19501 tcgctacact atccgtatat tcaataattt catttttcac attataggtc gttcggctta
    19561 gatataatac cggggcacca tcagcgatac gaagcggatc agcatctttc ttttgaacga
    19621 tacttgcata aaattcttca tctgctaatt ggacttgctg ttcataatcc tgagaaaaca
    19681 catcatacaa tggcttactt tcaagtaatg gcagatcaag accaataaat agttttgcag
    19741 gtaaataaga acgttccacc atcaatggtt cgtcatccgc taaacgaagt cttttcaatt
    19801 tgaaaactgt ttcgcctaaa ttgatgttta gattcttggc aacctttttg tttgcttcca
    19861 tttgcttgaa ttccaagatg actgtattag gcacccgtcc caattccctc atctcttctg
    19921 taaaactata cgctcccgcc agatcagacg acttatctga tagatcagac acatatgttc
    19981 cttttccgtg tcggcgataa atataccctt gcgtctctaa ttcttgcaaa gcaaggcgaa
    20041 ccgttgtccg actcactcca taatgctgac aaagttctct ttcagataac aatttgtcat
    20101 gaggtgtcat ctgtgtttcg atacgttctt tcaatacgtc aactagttga taatataatg
    20161 attttccttt acttgagctc tccataaaaa tacctcttct ttactggtta taaccacaac
    20221 acaagaatac ccacatattc ctcatttgtc aaagggtttt tggcgtaaaa aagctattat
    20281 aacaaggttt caaggtaatt tagaactggt atcaaaagat acctcaaaaa taccagttga
    20341 caaataaaaa acaaaaaaaa gctgaatctt ggcaaagatt cagctaaatg gtctagagaa
    20401 gtaaagaaaa aatattcttt atacatctgt ggctatttct actatcgaca agcaaatggg
    20461 cctaatagac tactatgtaa ataaaaatgg ctttctctaa ataaagtagg attttttatt
    20521 ttttagataa cttttctctc atgccggtct gatagtcgaa attttgttgt acatttttaa
    20581 aaataacaaa aattttttat gataattctg tctttttcta gtttccttat ggcgtaagtt
    20641 taacgaataa gaatttagta
//
LOCUS       DAYYCW010000051        19619 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000051 DAYYCW010000000
VERSION     DAYYCW010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.051"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(449..1078)
                     /gene="udk"
                     /locus_tag="ACHAYA_001633"
     CDS             complement(449..1078)
                     /gene="udk"
                     /locus_tag="ACHAYA_001633"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM8605433.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1162..1857)
                     /locus_tag="ACHAYA_001634"
     CDS             complement(1162..1857)
                     /locus_tag="ACHAYA_001634"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM8605434.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1870..2586)
                     /locus_tag="ACHAYA_001635"
     CDS             complement(1870..2586)
                     /locus_tag="ACHAYA_001635"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM8605435.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2916..3524
                     /locus_tag="ACHAYA_001636"
     CDS             2916..3524
                     /locus_tag="ACHAYA_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM8605436.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3849..4172
                     /locus_tag="ACHAYA_001637"
     CDS             3849..4172
                     /locus_tag="ACHAYA_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8605437.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4354..5724
                     /locus_tag="ACHAYA_001638"
     CDS             4354..5724
                     /locus_tag="ACHAYA_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM8605438.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5854..7155)
                     /locus_tag="ACHAYA_001639"
     CDS             complement(5854..7155)
                     /locus_tag="ACHAYA_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8605439.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7509..9500)
                     /locus_tag="ACHAYA_001640"
     CDS             complement(7509..9500)
                     /locus_tag="ACHAYA_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM8605440.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9644..9910)
                     /locus_tag="ACHAYA_001641"
     CDS             complement(9644..9910)
                     /locus_tag="ACHAYA_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8605441.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10420..12546)
                     /gene="pnp"
                     /locus_tag="ACHAYA_001642"
     CDS             complement(10420..12546)
                     /gene="pnp"
                     /locus_tag="ACHAYA_001642"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM8605442.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12734..13003)
                     /gene="rpsO"
                     /locus_tag="ACHAYA_001643"
     CDS             complement(12734..13003)
                     /gene="rpsO"
                     /locus_tag="ACHAYA_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM8605443.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13254..13817
                     /gene="def"
                     /locus_tag="ACHAYA_001644"
     CDS             13254..13817
                     /gene="def"
                     /locus_tag="ACHAYA_001644"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8605444.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14070..14591)
                     /locus_tag="ACHAYA_001645"
     CDS             complement(14070..14591)
                     /locus_tag="ACHAYA_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM8605445.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14594..15436)
                     /locus_tag="ACHAYA_001646"
     CDS             complement(14594..15436)
                     /locus_tag="ACHAYA_001646"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM8605446.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15429..16154)
                     /locus_tag="ACHAYA_001647"
     CDS             complement(15429..16154)
                     /locus_tag="ACHAYA_001647"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM8605447.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16330..16626
                     /locus_tag="ACHAYA_001648"
     CDS             16330..16626
                     /locus_tag="ACHAYA_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605448.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16882..17056
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17194..18786
                     /locus_tag="ACHAYA_001649"
     CDS             17194..18786
                     /locus_tag="ACHAYA_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8605449.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18799..19539
                     /locus_tag="ACHAYA_001650"
     CDS             18799..19539
                     /locus_tag="ACHAYA_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8605450.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5616 a         4027 c         3717 g         6259 t
ORIGIN      
        1 caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga aataattttc
       61 gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc gttaaacggt
      121 gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg aggagctgtt
      181 ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct ctacttcacg
      241 gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata aacagctatt
      301 ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct cttgttataa
      361 ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt tcaaacggtc
      421 gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta gctacttttg
      481 ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg atatccgcat
      541 agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc aaatattgtt
      601 cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata cgacgaatga
      661 tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca cgcagacgtt
      721 cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc tgaatgatcg
      781 ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg gctttatagt
      841 tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat gggtgatcgt
      901 aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa taagaatctt
      961 gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg ctgacacttg
     1021 tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt tttgtcattg
     1081 actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa aaaacacgaa
     1141 aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat aaagttcctc
     1201 taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt tatttctatt
     1261 gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag tcaaagaatc
     1321 accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga catgatcaaa
     1381 aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat ccttgaagta
     1441 atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac cgttcgtcac
     1501 aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc ctggaacaag
     1561 atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac tatctactgt
     1621 tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt ctcgtgatag
     1681 tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc tggtttcgat
     1741 ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat tggcttcagt
     1801 taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat atttcatctt
     1861 tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg tcaacgatcg
     1921 atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta gccgtttcaa
     1981 tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga attttccttc
     2041 ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt ttaaatcagc
     2101 cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg ttacgtctgc
     2161 cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt tttctggaag
     2221 tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg ttgtatctct
     2281 atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa tcagatcgat
     2341 tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt tattgagaag
     2401 ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat gcagtaaact
     2461 atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt gcttcaaagc
     2521 aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta atttatctaa
     2581 acgcatgctg tttcctttct tattactact aattaattat accttgaata ttcttgcttg
     2641 caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac ggttaaatag
     2701 taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa gttgaatcct
     2761 gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc gccctgcctt
     2821 cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt ccgttaccaa
     2881 agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta gaagtaagta
     2941 aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg aaagttccag
     3001 ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt ttgacactaa
     3061 gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc gatggacaaa
     3121 aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat ttcgaacatg
     3181 cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa gtcgttcttg
     3241 ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg tatacagtag
     3301 tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg acaaacttgg
     3361 aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac aaattcgtga
     3421 ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa gaaagcacag
     3481 gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga aacaatcagg
     3541 tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc taatcgttta
     3601 ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc ctaatttacc
     3661 aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt gaaaaaaagt
     3721 attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg ttgaagtaag
     3781 tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat tcgaaggagg
     3841 ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca gttggtgtcg
     3901 tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac ctagatctta
     3961 agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat cgtggaaaag
     4021 tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat ttgccgtttg
     4081 cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg agagaaacga
     4141 tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg atgtccctta
     4201 ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc tttttatgga
     4261 gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct atatactact
     4321 aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa aggaagcccc
     4381 gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt tcagcagttc
     4441 tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc gctggcagcg
     4501 gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg tttaacagct
     4561 ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg tctgaagaaa
     4621 agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac tgtcctaagt
     4681 ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat acttgatcaa
     4741 gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag tttcaatttg
     4801 ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt tctgatcatt
     4861 gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg catgggtgtg
     4921 gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact gtgtctagtg
     4981 tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa atttgaaaaa
     5041 gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc ttcgatcatc
     5101 gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga tgtcgtagct
     5161 gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat gtcatttggt
     5221 attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg ccgaatcttg
     5281 caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc aggtgctatc
     5341 gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga aacacgtgta
     5401 tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat cttagcagtc
     5461 ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc tacgatcgcc
     5521 ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg aatgtgggga
     5581 tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc cgtgttgctt
     5641 tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga acatttcaca
     5701 ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc taaaaatgaa
     5761 aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga taacaaaaag
     5821 gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt ccgtggactc
     5881 tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac ggaatgatgc
     5941 aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa agcccgcaag
     6001 tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac gctggaaatc
     6061 atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg ctatcaatgc
     6121 ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa tatcttcaat
     6181 cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat gcttaccctt
     6241 ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta cgatcggctc
     6301 ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga tcaaatctgg
     6361 caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat agcgagtatg
     6421 tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt cgaatacttg
     6481 catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt caaacacgtt
     6541 ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat tcaatgcgac
     6601 taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac gcacacctaa
     6661 tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa acattacttt
     6721 ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat tgacgatact
     6781 atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta aaataaatcc
     6841 cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac cgatcactgg
     6901 ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac cggttagcgt
     6961 aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat gaagagctag
     7021 cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc tgattttcac
     7081 taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa ataaaataat
     7141 gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt tttctacaaa
     7201 aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg gcatttttca
     7261 gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca gctttttcat
     7321 gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc caaacagatg
     7381 aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag tacagctttt
     7441 tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt caggtcaaag
     7501 cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat gataagcgga
     7561 acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg cgaggtagcg
     7621 atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc tagagttgtc
     7681 acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc tcgccctggt
     7741 gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa atcatggatg
     7801 acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa cagattcact
     7861 tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata tggctcatcc
     7921 gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt atctaaaatc
     7981 gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa gaagaccaca
     8041 tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg cgggatataa
     8101 tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg ttcccaaata
     8161 aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc tcggaagaat
     8221 ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa ggtaactaaa
     8281 tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag tgctgttgtc
     8341 atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg agacgttcgg
     8401 aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc tgggatatac
     8461 atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag tttcaacttg
     8521 attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc aatgactgca
     8581 gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat ttggaagaat
     8641 ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa caaccacgca
     8701 gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct cagacgaata
     8761 tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt cgtagccagg
     8821 aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag cagctggata
     8881 gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt cattaatccc
     8941 attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt tccgacaata
     9001 tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt gataatgacg
     9061 atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg caagccttca
     9121 atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa ggcagctcct
     9181 ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt gtagagtgag
     9241 aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata tttgattgtt
     9301 gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa atcaggattg
     9361 attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag cggacttgtc
     9421 ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga tatctttttg
     9481 gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata tcctttaggt
     9541 aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa ttgcttctaa
     9601 ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct ttctttggac
     9661 gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt gcacccaata
     9721 atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt aaaaattcgc
     9781 cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc gggacttcct
     9841 ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac gaccataaaa
     9901 aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt acgctttatt
     9961 cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt gtaacaaaag
    10021 tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag gttgtgaaat
    10081 cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca tattttgccg
    10141 ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag aggttgtaaa
    10201 aaagctgttc tgcatcaagt aataagaaca gccaagtaaa aacagctcct aatgttttta
    10261 acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt cgaaaaaaaa
    10321 ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg tccccctgtc
    10381 accttcacga tcacaagtac attattccta tgaattaaat tatttttctt catttttcgt
    10441 tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt gtttatcgat
    10501 ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga ctttattgac
    10561 acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga tcaagttgac
    10621 gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac caactttgac
    10681 ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg catcagctga
    10741 agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt catcgatgat
    10801 tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag gttcgatctg
    10861 gatcatttcg atttttggtg catattggct taattcttca cgtggagcag caatcgttga
    10921 agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg cttcagttag
    10981 gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga taccatcttt
    11041 tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt ggatatcagt
    11101 taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg cgactggcgc
    11161 tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac ttgcttggga
    11221 agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag gaaattcttc
    11281 ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc cgatttcccg
    11341 acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga aattgtagtg
    11401 gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt gctctcctag
    11461 aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca aacctgatcc
    11521 atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat ccaatttacg
    11581 cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt tttcaagatc
    11641 ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac ctgcaaattt
    11701 ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt cgcgagcaag
    11761 tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa agatttcttt
    11821 tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc ctactgcttg
    11881 aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa acagcaatgc
    11941 accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat tgatggcttg
    12001 tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta ctgttggatt
    12061 caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc cgtcaaatgg
    12121 gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag ctggcgagca
    12181 gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga agccttctgc
    12241 aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt ccgtacttgg
    12301 acggccttct cgtttgatga aacctccagg gattttccct gctgcataca ttttttcttc
    12361 ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg ccacagctgc
    12421 acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt gttttgctag
    12481 ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata cttgtttttc
    12541 tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca aatgaaaact
    12601 gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc tagcttgatc
    12661 acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg aatcccactc
    12721 tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg taacgttgaa
    12781 cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt ttcattaggc
    12841 cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag tggttgattt
    12901 cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca tggcgagcgt
    12961 attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc acctctttcg
    13021 attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg gcaggtcatg
    13081 tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag tagttaatca
    13141 ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg tggtaaacta
    13201 taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat tccatgatta
    13261 caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca aaagaagttt
    13321 ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag ttcttaaaaa
    13381 acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt ggcttagctg
    13441 ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt cctgacccag
    13501 aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg agccattctg
    13561 ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga gaagtaccag
    13621 gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat ggtgaaaaac
    13681 acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgagatc gaccatatca
    13741 acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta aaagaaggcg
    13801 tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca agaaagcttt
    13861 ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc cactaatcga
    13921 aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg gatacgcatc
    13981 ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg atagaatagt
    14041 ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc tgcttcagct
    14101 agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt atgcagatgg
    14161 atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg tctcataaag
    14221 gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc gtaaagggga
    14281 tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct cagttcttct
    14341 tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt attggtttct
    14401 tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc agctcgtagt
    14461 aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc aaacttgcta
    14521 gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg ccgtgtctct
    14581 ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag tgtttcatca
    14641 aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc agactaacaa
    14701 ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt tcttcttttt
    14761 tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca accaactctc
    14821 gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt agttcttcct
    14881 ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg ggaaaagatt
    14941 tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca cccggcgtga
    15001 cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt gcgtactcca
    15061 gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc tggacattcg
    15121 gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat gtacaattga
    15181 aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa ttgataactg
    15241 gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat tgggatcgca
    15301 gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac atacggtcgg
    15361 caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt tctttttcgg
    15421 ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac aaccgctccg
    15481 tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt gattactaag
    15541 attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac ttgaaaactc
    15601 gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg ctccatgact
    15661 ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc tccgcatgct
    15721 tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca ttttcgacaa
    15781 tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc ctttgcgtgc
    15841 aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag atgtgctatt
    15901 tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac aaattgatag
    15961 aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg ctgctccatc
    16021 ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt ggatacccct
    16081 gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc ttcgtctact
    16141 gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc gaacaaattt
    16201 attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg agcaatttat
    16261 cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat aaaagtgagg
    16321 tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg tatcgcctgg
    16381 ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa tggaaatctt
    16441 tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac attagccgta
    16501 tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct aatattagcc
    16561 atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa gaaagcggca
    16621 gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt ctttttttat
    16681 tgtattacat acatttttgt gtataattat actatcgttt gcaatggata gatgattcac
    16741 attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat tttttcaaaa
    16801 accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt gtataaataa
    16861 ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta accctgtgga
    16921 gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca cttttttgct
    16981 tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag tgatgctaag
    17041 ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc tttttatatc
    17101 tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca tctagaccgc
    17161 tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt ttcagttgcc
    17221 gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa tgcagatgaa
    17281 aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc cttcaactgg
    17341 tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag ttacgccggc
    17401 ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa attagtcatc
    17461 gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat cgacgcgatc
    17521 atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac cgatccttat
    17581 tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa agcaaaaagt
    17641 ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca ctatactgtg
    17701 atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc agcaatgaga
    17761 acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga aggtgtaacg
    17821 gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga tggcttccag
    17881 acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc ggatatccaa
    17941 aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat tatggaccaa
    18001 gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat catttctgat
    18061 ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg acttaccttg
    18121 ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat tggtgtcttc
    18181 cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat cggaaatgca
    18241 cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca agcaatggtc
    18301 atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat tgccgctttg
    18361 tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg ggggattttt
    18421 gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca tggccaaacg
    18481 atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac aggaaatgag
    18541 tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc tgatttgttc
    18601 ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt tacgatcgtc
    18661 gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc ggtagaaaag
    18721 aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc aaatttgcag
    18781 gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct aagaaaaagc
    18841 ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg tgaagttgtg
    18901 acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat caatttactg
    18961 gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc tcctaaatat
    19021 aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa cctgttcaac
    19081 aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa acgagataaa
    19141 gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga tcgttatatc
    19201 gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat tgctcgggca
    19261 ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt agaccctgaa
    19321 atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt gacaatgctt
    19381 atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat ttttatggat
    19441 aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc gaaagaagaa
    19501 cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct gattgggaca
    19561 aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag gtaaatttc
//
LOCUS       DAYYCW010000052        19502 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000052 DAYYCW010000000
VERSION     DAYYCW010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.052"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..3654
                     /locus_tag="ACHAYA_001651"
     CDS             127..3654
                     /locus_tag="ACHAYA_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8605451.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="ACHAYA_001652"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="ACHAYA_001652"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM8605452.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="ACHAYA_001653"
     CDS             7442..7783
                     /locus_tag="ACHAYA_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8605453.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="ACHAYA_001654"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="ACHAYA_001654"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM8605454.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /gene="pheT"
                     /locus_tag="ACHAYA_001655"
     CDS             9140..11560
                     /gene="pheT"
                     /locus_tag="ACHAYA_001655"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM8605455.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACHAYA_001656"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACHAYA_001656"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM8605456.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="ACHAYA_001657"
     CDS             12994..13731
                     /locus_tag="ACHAYA_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8605457.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="ACHAYA_001658"
     CDS             13745..14560
                     /locus_tag="ACHAYA_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8605458.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="ACHAYA_001659"
     CDS             14573..15220
                     /locus_tag="ACHAYA_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8605459.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="ACHAYA_001660"
     CDS             15236..15892
                     /locus_tag="ACHAYA_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8605460.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="ACHAYA_001661"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="ACHAYA_001661"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM8605461.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="ACHAYA_001662"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="ACHAYA_001662"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM8605462.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="ACHAYA_001663"
     CDS             18234..18752
                     /locus_tag="ACHAYA_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8605463.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /gene="cbpB"
                     /locus_tag="ACHAYA_001664"
     CDS             18859..19359
                     /gene="cbpB"
                     /locus_tag="ACHAYA_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM8605464.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6562 a         3348 c         4184 g         5408 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    19501 tt
//
LOCUS       DAYYCW010000053        19426 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000053 DAYYCW010000000
VERSION     DAYYCW010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.053"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(248..988)
                     /gene="truA"
                     /locus_tag="ACHAYA_001665"
     CDS             complement(248..988)
                     /gene="truA"
                     /locus_tag="ACHAYA_001665"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8605465.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1179..1814)
                     /locus_tag="ACHAYA_001666"
     CDS             complement(1179..1814)
                     /locus_tag="ACHAYA_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8605466.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(1961..3181)
                     /gene="sstT"
                     /locus_tag="ACHAYA_001667"
     CDS             complement(1961..3181)
                     /gene="sstT"
                     /locus_tag="ACHAYA_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM8605467.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            3400..4398
                     /locus_tag="ACHAYA_001668"
     CDS             3400..4398
                     /locus_tag="ACHAYA_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605468.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            4594..6429
                     /locus_tag="ACHAYA_001669"
     CDS             4594..6429
                     /locus_tag="ACHAYA_001669"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8605469.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6489..7205)
                     /locus_tag="ACHAYA_001670"
     CDS             complement(6489..7205)
                     /locus_tag="ACHAYA_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8605470.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(7432..7974)
                     /locus_tag="ACHAYA_001671"
     CDS             complement(7432..7974)
                     /locus_tag="ACHAYA_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFM8605471.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(7979..8206)
                     /locus_tag="ACHAYA_001672"
     CDS             complement(7979..8206)
                     /locus_tag="ACHAYA_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFM8605472.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8285..10165)
                     /locus_tag="ACHAYA_001673"
     CDS             complement(8285..10165)
                     /locus_tag="ACHAYA_001673"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8605473.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(10354..10968)
                     /locus_tag="ACHAYA_001674"
     CDS             complement(10354..10968)
                     /locus_tag="ACHAYA_001674"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8605474.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            11110..11670
                     /locus_tag="ACHAYA_001675"
     CDS             11110..11670
                     /locus_tag="ACHAYA_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8605475.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(11975..12289)
                     /locus_tag="ACHAYA_001676"
                     /pseudo
     CDS             complement(11975..12289)
                     /locus_tag="ACHAYA_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(12543..14178)
                     /locus_tag="ACHAYA_001677"
                     /pseudo
     CDS             complement(12543..14178)
                     /locus_tag="ACHAYA_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14617..15486)
                     /gene="scrK"
                     /locus_tag="ACHAYA_001678"
     CDS             complement(14617..15486)
                     /gene="scrK"
                     /locus_tag="ACHAYA_001678"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8605476.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(15506..16627)
                     /locus_tag="ACHAYA_001679"
     CDS             complement(15506..16627)
                     /locus_tag="ACHAYA_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8605477.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(16629..17024)
                     /locus_tag="ACHAYA_001680"
     CDS             complement(16629..17024)
                     /locus_tag="ACHAYA_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8605478.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(17043..17840)
                     /locus_tag="ACHAYA_001681"
     CDS             complement(17043..17840)
                     /locus_tag="ACHAYA_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8605479.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(17842..18630)
                     /locus_tag="ACHAYA_001682"
     CDS             complement(17842..18630)
                     /locus_tag="ACHAYA_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8605480.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(18644..19120)
                     /locus_tag="ACHAYA_001683"
     CDS             complement(18644..19120)
                     /locus_tag="ACHAYA_001683"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8605481.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(19192..>19426)
                     /locus_tag="ACHAYA_001684"
     CDS             complement(19192..>19426)
                     /locus_tag="ACHAYA_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8605482.1"
                     /translation="SFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQ
                     THFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         6131 a         3908 c         3022 g         6365 t
ORIGIN      
        1 ttcaagatat ttatctatcg ttactttgat tttattttta ctttatcgat ttactttttc
       61 taaacaaaaa caaccgagaa gagaagtaca acattctcac tagattttta tttaagaaag
      121 actttttagc tgtacacata aacaggctaa aaagtctttt tttatatttc tttaatttgc
      181 ttcatacatt atttaacatc gatatttata ctgtgaaaca tgcgtttcat ccattatcaa
      241 agtattttta tcgataataa acttcatcca aaaacagacc ttgtgcaggg acggtttctc
      301 ctgcatgttg ccgcactttg ctttcaaata tttcatcgat tgtttctatc ggcatggcac
      361 ctgtgccgat ttctagtaat gtccccatga taatccgcac cattttatat aagaatccgt
      421 ctccgacaaa agtaaaatga agcatttctc cctttcgttc gatagataat tcatcaatcg
      481 tacgcactgt ggattttttt gattttttca atgcagaaaa accaatgaaa tcatgtgtac
      541 ccactaattt ttcgcaagct gcattcagct tgtccatatc caactcttga gggaaataaa
      601 agctgtggtt tcgctcaaat gctgaaggaa tcacgttgtt ccaaacataa tagctgtatt
      661 ttttgcccac cgcattatat cttgaatgga agcgctcagg catttcttcg attcttttga
      721 tgacaatatc ccgcggaagg taacgattca taaagtcgat catttctgcc agtccaattt
      781 ctgtgtttgt tttaaaattt gctacttgtc ctcgcgcatg ggtgccagca tctgttcttc
      841 ctgatccaat aatttctatc tcttcatttg tcatctgatg caatactttt tcgatttttc
      901 cttgaatggt tttatcgtca tctccaagtc tttgccatcc tgaatatctt tttccgtcat
      961 attcgattgt caacttgatg tttctcatat tttagtggct cctctgtctt ccatttggtc
     1021 ttctctctac ctttttaaga ttgtagcaca aaatatagct ccttgcttct ctaagacaga
     1081 agctatacaa gaaaactgtg gaaatatata tttcagaaaa acaaaaaaca gccaccggat
     1141 gaagtatccg ctgactgttc aatttgacgg ctcaagtttt actgtttgag atgcaagata
     1201 ctcaaccagg caatgggcac ataattaaag aaaaaactga ttctttggca aaaacctatt
     1261 ttattcagta atggccaaga atttatagaa ggaattcttg ccagtccata aatcgccgca
     1321 aaaagcaaac tgatcacaga cagtccaagg tataagtggc ttacgtccat ttgcccattt
     1381 tttcgataga tcaagaaaag aagaactgga aacaataaaa aaccactgta acctaaacct
     1441 gatccgatat tatggaccca tgtcgagaca ttccacgaag attcatgggt attgatactg
     1501 aacaaacctg taaagataca atcacctata ccatagaacg cgatggctgc tgtaactagg
     1561 gtagctaaca cacgagatgt actaacaaat gcagcataca atgcaggcaa tgacaggatg
     1621 aaaaagatcc ctgaaaccac agaccaaata agaaacgctt ttcttacagg actggaaacc
     1681 tctccaaata cactgatcac gctcttcatt tgatctaatt caggataata caatcctaat
     1741 acgtacggag tcaaaaaatc actcagcacg cctaataata aaaagaaaaa accaaattta
     1801 cgtaaaaaat ccatgatacg cctccaataa caattaccta tgcttttaat tgttattata
     1861 aacgacttta tcttgattta acagaaacct ctatttacac gaacatctat ataactcatt
     1921 aaaaactcga atcattttaa aaatactaaa tatctaacgt tcaacgttca cttaatccat
     1981 ctaaacaatc tttcaatttg attttttccc cacttctttt acgcgcgcct agctctccta
     2041 ttgcagtaaa caacagatca cttgacgaat ttaaggcagt ttccattgaa tcctggataa
     2101 caccgataat aaatccaata ccgacgattt gcatcgcgac atcattagag ataccaaaca
     2161 aactacatgc taatggaatc aataacagtg aaccgcccgc aacacctgaa gctccacaag
     2221 cagctaaagc agacaaaata cataaaagga ctttcaaaac aaaaggtgct tcaatcccta
     2281 aagaatgtac agcagtcaac gtcatcaagg aaatggtaat cgcggcaccg cccatattga
     2341 tcgtagcacc taacgggatc gatatggaat aagactcttt tgtcaatttc aatctttcag
     2401 ccaacatcat gtttatcggg atatttgcgg cagaacttcg ggtaaagaaa gcgggaagtg
     2461 cgctttcttt caaacaaaag aaaatcaatg gatagggatt ttctttaata aacaaaaacg
     2521 tgaggaatgg gtacacaact aatgcgacaa acagcatcgt accgatcaaa acaagtaaaa
     2581 gctgcagata ctctgccaat ccagcgattc cagtcgtagc aattgattga taaaccaatc
     2641 ctaaaatccc tatcggagca aatgcaataa tcacttgtac gacttgtgac aacgctatag
     2701 atatctgatc caccaactct tttgtcgatt ctgaacgtgc tcgaaaagct agtcccaaca
     2761 atacagccca gaataagacg ccaaggtaat tcgcatcaat aagagattga acaggatttt
     2821 gtgtgacatt cgtcaacata gtagataaaa cactttccaa actttgcggt gctttttcag
     2881 atactgtttc ttgtaaagta ataggaacag taaaaattga acttacaata actgctacaa
     2941 gtgcagaaag caatgttccc actaaatata agatcaaaat aggcttcacg aatgtcttat
     3001 tcccaatttt atgcttggca attgacgcca tcgtcaagaa aaaaaccaga ataggtgcga
     3061 ttgccttcaa gcaactgaca aacaacgttc ccaatattga aataaatgtc catgtgggga
     3121 aaaagatacc aaacaatatt ccgactaaaa taccaacaac aatacgttga actaacgaca
     3181 tatccaaaaa cttctctaac aaatgaccac cctttcacaa ttttaacaat cattgtatca
     3241 ttttatgcaa agaaactaaa ggtataattt tctaaaaatt ctaacaatac aaacaaccat
     3301 ttcttgctaa ttttgtactt tttctgtcta cacgttgttt tttgagatat tctaagttag
     3361 gatgttatta atgggcaact tatcaggaaa cgttttctta tgtttttgtc caattttttt
     3421 gtgaggtgtt tattcttgaa aaaaataaag aaggagagtt atggcttcct gacaatcttt
     3481 gtcgcttgta taatgatggt tttacagcgt atattgattt cagcagtcac tattcctaca
     3541 tatgcaacaa accctggtcg attctacatc tatacatttt tacaaacttc cgttgttatt
     3601 ggtgcaaatt tgatcccact ttacatgggt taccaatatc aaaaaatcgc ccgtttaaaa
     3661 gttttacatt atttaagtcg ttttgccttt atattcctaa tagccacact agtttgcaat
     3721 atcttttttt acgtacaagc tggcagcttg aatattcgtg attattggct tatcctaaca
     3781 cctatcagtc agaattactt tacttatgct gtttcttgta ctttgctttt ttgtagtatt
     3841 ccttatacag ttggattttt aaataaatta tcgaaaacaa caattaaata tctactatta
     3901 tttttaacaa ttggattagt tggctgctct actctattta ataaagaccc gtttgcatta
     3961 cagaatggac agagtatttt atggattttt tatttatttt taatgggtta tggtttgaaa
     4021 gaatggaact ggaaagaaaa ggtccgctat aagcttcctc aagtatttat cagcttgatt
     4081 gttttattcg gtttgattat tttgatgaca caaatttcac tcatcatcag aggaaataca
     4141 gatacagcgc tgaggttttc taaaccatat tctctcttcg caatgtatta tactttctct
     4201 tcgtttttat ttttagaaat gctatctgat aaattaggta taaaaggaag aggaaatttt
     4261 cttgctgtat ttttgattgt cactcaagtt atatgtaatt tccctttagt ttcttatcgc
     4321 gtatctacat ttatgaaacg tgaatttcca aactcaggga aggcatggct gataaatatc
     4381 gggcagtttt ttatttgata tttattagca gtgtccctcc tagttatctt tttattttat
     4441 atacaaaaaa tccgtttcat tcatcgtttc atcgaatact tcgcttttga ttcgattgaa
     4501 caattaactc agcgttggaa aaaaagaatt cattggcttt ttgttcataa aagttatttt
     4561 ttagttgcag cattttttta ctgctttacc ttcgtgcaga ttttcgtttt agaaccaaaa
     4621 gaaggttgga aagaaactat ccaggttgcc ttaaaaatat ttactcagcg acaagcaccc
     4681 atgatcctga caatcatcat tattatggga tttttcttct tactattgtt gatttccaat
     4741 cgtttttggt acgccttgac tgctaccttg atcattaact tactgctcac tatttcgaca
     4801 gtaatcaaaa tggaaatgcg ggaagagcct gtctttcctt cagatttaaa aatgctgacc
     4861 ggattgtcag aactattatc gatggttagc ccagtcttat taatcgtggg cggattgatc
     4921 cttctcctat tattgattac tagtattatt gtacaacgta gactacaaaa acagtattcc
     4981 ttgaagattc attggaaacg tcgatttatc agtatcgctg tattattagg atgtttttcg
     5041 ggtgtctttt tcattaatca caaaaattcg ccttctttct tgctgtttaa tttatttaaa
     5101 gtaaacaaga ctttctttaa ccagcaaggc gccgtaaaag ataacggacc agtcattcaa
     5161 ttcctaaaca atatcgacat taaaatcatg gaagaacctg ctggctattc gaaagaaaaa
     5221 attaatggaa taatgaaaaa gtacaacaca aaggcagatg aaatcaacca aacaagaagt
     5281 gactggttaa acaatcagac gatcgttctc aatttaagtg aaagcttcag tgatcctgca
     5341 cgtgtcccta atctgacagt acctacagac cctattcctt acatcaaaag cattataaca
     5401 gaaggaacag gaggattgat gttatccgtc ggttatggtg gcggaacagc taacatggaa
     5461 tgggaaggac tgacaggctt gagtatcagt aacctgtctg cttcgttagt tacaccttat
     5521 acccagctag tggagcgtca gaaaataagt ccaaatatta ccgatctatt cgatgaaaaa
     5581 atagccatcc atccttttac agctgctctt tatcgcagaa aacaagtgtt tgataaattt
     5641 ggttttgaca ggttctatta tttagacagt ccggataaat taacttatac ggataaaata
     5701 gaacaaagcc cacgcatatc ggatgattca gcatacaaag aaaccttgaa agctttacaa
     5761 agcaatacgg aaacgtcaca attcatccaa ttatccacta tgcaaaacca tatgccttat
     5821 gatgattact atagtgaatt gaactacact gctgaaggaa ctgctgtcat tcctaataga
     5881 agaaaagaat tacaaactta tatgcaaggc cttcactata ccgatacagc agtaaaaaaa
     5941 ttcatccaag aaatcgacaa aatcgataaa ccgattacct ttgtttttta tggtgaccat
     6001 ttacccgcaa tctatagtgg gaatgattcg aaaaaatatg gattagaaca gcaccagaca
     6061 gattatttca tttataacaa caagtatagt cgtgaacaag cagctacgtt aaataaagaa
     6121 gttgttgctc ctaataactt tggtgctatg gctcttgagc atgcgaatat caaagtaact
     6181 ccttactatg cgttgttgac agaagtatct aatcatctac ctgcattaac aattgatcct
     6241 acagaaagct tgtctaatcg ttttaatgga aaacaggtct ttgttacaga ggatagtcaa
     6301 attatccaag aaaaaaattt aactgagaaa caaaaagaaa ttctagaaga ttatcgtctg
     6361 atacaatatg atcttgttgc aggtaaccag tacagtgcaa catgggcgac acaaaaaata
     6421 aaagactaac aaataaactg agcaaggggt tgtgataaaa gacatgtcac aaccccttgt
     6481 ttatctcatt aatttgcttg tccataaacg atttgattaa agccattttt caattcatta
     6541 ttcgctgtct cgttgaagta aagataatta tcgggattcc cctgattttg ttgtgtcacc
     6601 aatacttttg gctgattttg ctgaaacccg aatagataga cgtgcttttt cagatatggt
     6661 tctgtctctg catcggaata gacggctgtg atttgatagt ctgcttgacc tgtcccactt
     6721 tctgaccatt ctgcagaaac aggcactcca ccaatagcca tcttccactc cccgttcaaa
     6781 atagcttgag ggactttaag accatataag tccacactca tttgattcgt ataacttttg
     6841 tattgttgtc ccattgtttg gccccatttg gtcataaatg cctctaattg tgcggatttg
     6901 tctgcattcc acagtgtttg ttcaacttgt tcgctgcttt ctgaagagga agatgaagcc
     6961 tctacagaag aacttgaagt agatgtttga gttgtttcag tcgttttgga agactctgac
     7021 gaagtagagg tatctgctgt ttctgtactt gctgcagttg acttgctctc tgtgctagtt
     7081 gttgtagtag ttgcaactgt tgctttcgct gtttcttttg ttgattcttt tttcgattca
     7141 ttcccgttgg tacaaccagc taacaaaaaa gctagaccaa ttgttactaa aatccttttt
     7201 ttcaattaaa aaactccttt cgattactcc cacttcttta ttctatcaaa taagcaatgt
     7261 gattttattg atttttcgta ttttctcatc aatctttcaa atcttctaaa aactgagaag
     7321 cacccgctga tgttgtctgt aatacatcag cgggtgcttc ttttatttta atcttcgtga
     7381 ttttccaatt tacctcacgc tttatatttg tatttgaaga aacatagact tttagtcatc
     7441 atcgtcttcc tcatccaaat cttgtcggac agtttttcct tggagtcttt gtacttggta
     7501 gatgatttct ttattgaaac ttaaaagttc aacaagcaat tgttgcaagc catatttttc
     7561 ttctttttca gctaatttga ttgcccgtgt gataaagaca ttttggaaat caaaatcttt
     7621 tgcaaagttt tccaacattg ttgaggctgg taaatatttg ttttgaccat gttcgtaaat
     7681 ttttccataa cgcatgactt cttctaaagt agacggaacg atttcatttt caatcactaa
     7741 ctcatgagag attttattga accatctcgc ttcttgatca gcatattctg aaaaaagggt
     7801 acttaattct tgcgaagatg tacccaaaac atacaattgt gcttggatca atttcatttc
     7861 ttctaattta aaatttgcta atacatgccc caccatggcg ccagcagttg gtttatgatg
     7921 gtcgatatct gcttgttttg tttcttgaac aaatttttct tccactatat tcattgattt
     7981 acgcctcttt tacttttgct gattggactt cataacctaa tttttccaca actgcagcta
     8041 attgttcagg gtggacttca tctggatcaa agtttgcttt gattttgctc gcattaaaca
     8101 aaactttcgt attgcttacc ccaggctgtt gagaaaccgc atgttcgatt ttttgcatac
     8161 agcttggaca agctagtgta tctaattgca taataatttt tttcatcgta gttcttcctt
     8221 cctttttctt gatagtctta ttctagatta cagcttccat aaaaaaattg attcttatca
     8281 agttttacga tttttcttct ttgctttcgt gtccaaaata caataaacgc atcgcgttaa
     8341 agataacgat caagatacta gcttcgtgaa cgaacatccc actagccatg taaataaacc
     8401 cggcaaataa gccgttgagt aaaaataaga cgacaccaat ggcaatcact acattttcac
     8461 gggtatttaa aacagttttc ttagccaagc ggtaggcatg aaccaacgat tcaaaattag
     8521 attgcatcaa gacaacgtca ctcgtttcga tagcaacatc cgttcctgat cccatagcaa
     8581 tcccgatatc agccgcagcc aaagagggag aatcattgat tccatctcca ataaaagcaa
     8641 cgcgcaatcc ttgttcttgg tattttttca caaattgaac cttttcgtct ggtaataatt
     8701 cagcatggac ttcatcaatg cctaacatat cagctacata atcagcagtc atctgattat
     8761 cacctgtcag catcactaac tgttttgctc ctgcttgttt caacttagct aattgttcag
     8821 cgacttctgg tcgaatcaca tctgacacac ctatgatttg agtgattttt ttgtcgattg
     8881 ctacaataat gaccgagctg cctgtttgtt gtaattgatg taaatcttgt acttgctttg
     8941 gtgtgagaga aagtttgtga gcggcaatca atttggtgtt gcccgcacaa actgtatgac
     9001 catcaatctc agctgtgatt ccttgtccct tgattgtctg attatctttg actgctaatt
     9061 gttgaaaatc aatgtctttc tcctgagcat aatggatcac ggcgcgcccc agaggatgat
     9121 cggataacgt ttctaatcga gccactaaag ctaagctgtc cccttcatcc tctgtatagt
     9181 tcttgatagt ggatacagca gtagttcctt tggtcaatgt accagtttta tcaaagacaa
     9241 aagtatcgat tttggaaaaa gtatccattg cttcaccgcc tttgactaag atgccccgtt
     9301 tcgctccatt accgattcct gctacattcg aaacaggcgc accaatgact aaagcgccag
     9361 gacaaccaag aaccaaaaca gtgattgcta aacgaaaatc acgactaatc agaccaacaa
     9421 ccaacgcaat aagtaaaacg gttggagtat aatactgaga aaatcgatca atgaattttt
     9481 ccgcatggga tttggtatct tgtgcttctt ctactaattc gataattttg gcaaaggtcg
     9541 tatcatctcc gaccttagtt gcttcaatcg tcacgtagcc atctgctaaa atcgttccag
     9601 agaagacttc atctccactg attttgacga cttgtcgtga ttcaccagtc actgcggctt
     9661 catccgcata ccctttcccg tcaatgactc gtccgtccac aggaatagct ccaccagttt
     9721 ttactaagac gatatcgcct tcttccacgt catcgatttc cacttcttca gtcgttccat
     9781 cttcttggac aacactagca gtagatgggg ccatctcggt caaagccttg attgactccc
     9841 gcgttttggt caatgttttt tgttccagat agctaccaaa caaaaacaag aatgtaacga
     9901 tagctgactc cttgtattct ccgatcataa aggcaccaag cacagcaata gtaacgagta
     9961 aatcaattga gataactttg actttcactg cctgatacgc atggatcata ataggtatga
    10021 ccgcaataat agaagctatg gctagagcaa tgttatagaa caagacaaaa ccagtaaatt
    10081 ttccgataaa accaaaaata atcaacacag ctgataaggc ggtgatatga tttcgatgct
    10141 cttggatcca cttttgtaat ttcatctttc tccctctttt cctttcattc ttacgagact
    10201 aagtatagat acttatccat cataaaaaat tgaccactat caagtttagt caaaaaggcg
    10261 gttttctttt ggtagaatac actacaaaaa atagttagca agaagactca ctaaaagtga
    10321 tcttgccaac tattgtcgtt tctatcgaaa ttttcattga atgattttcc tgtacagcat
    10381 acatcggaga tctatttctt tatctaagat ttttatcttg ctaatttttt cctttagtct
    10441 ttcaaatcca cgtcgttcat agaattgata cgtacagcca ctatctgtaa aaaggaatat
    10501 ttcttttcct ttctctcttt tttcaaattc tgttagcaac ttggtaccga tgccttttgt
    10561 tttcaaatca ggatttgcag ctaaaaaaat gatctctcca tctggcgaat gcttctttaa
    10621 atatgctgca agcatttgtt catttgctgt ctcgtaagct cctttacttt ctttagcaaa
    10681 tatattttgt aaaaaatcga atatcttaac atatatttgc tgtccaaaag aacgatgttt
    10741 ctttttttct ccttttatct ccgccagcaa aacacctgcc aactcgtcct ctgcatacaa
    10801 agcaagaatt tgagttgctc gattcaactc catatacaaa aaatatcttc cgtaaagatt
    10861 cagcagaagt tcattttcca tatatctctc aaagttcatt ccttcaacag caaaatagat
    10921 agcttttttg taatctttct tttctaaggg tttaaaagtt agtttcatca tttcgcctcc
    10981 tcaacttcaa tgattgtgtt atacttacat tataatagac acagtgtcta ctatctataa
    11041 tcaatcatct ataagtttgt cgattaagcg acagctttgc tttgatatgt cggataagga
    11101 gataggtaaa tgaaaaagac agatagacgc gtcaaaaaaa cagaaaaagc tttggctgaa
    11161 acactatcca cattattagt aaataaaaag attcaagcaa tcactatacg cgaactaaca
    11221 gaaacggcag atgtacatcg ttcgactttt tatacacatt ataaggatat ctatgatcta
    11281 tatgatcaat tagaaagcaa cttttttcgg gatctaaatg aaattctctc ttatgatcca
    11341 gcccatagct atgaggaact atacactcgc ttgattgact atgtttacgc aaatactttt
    11401 ttatatcgct tatttatcct caaccatggt gatatttcgt ttcagaagaa agttaaacaa
    11461 gttattgaag aaaactatct gaacatatgg ctatatgaag acaagaaaac gattatcaca
    11521 gaagaaatga gattctttac tacgtaccac gtccaaggtt gtctatccgt tctcaatcgg
    11581 tgggtagaaa gcaattacaa gtactccaaa aaagaattat tagaactctt acagaaactg
    11641 aatgatcaca tagagaaaat catgccttga ataaaaacat actaagctta gttaagaaga
    11701 accgtaacct tctcgtataa caactgaatc ttatttagcc aaattgttta ctgaagattt
    11761 tttgagtaaa atttctataa ttcctactta ttgaaaagta gtttgtagtg ctgcttctcc
    11821 ctctctaagt gatatatctt gttgtaccaa tcaaattcac ctcatcatta ataaattact
    11881 tttaggctaa tttattacaa cttataggtt gaagctgtaa ccaatttata gtcatattat
    11941 atttttatca tccagtttat tgaattcttc tcgtttacct caattcattt tcaaggggaa
    12001 tcagctcatc atctaacatt ttacgccatg ctcgcttcat ttcttgtggg tatttaaatg
    12061 gtttcgcttc tggatgtttg gctttatatc cttcgtaatc cttactgttg ataattgctt
    12121 tcatattagg aattggatct tcacctggca tatattgcag attagaagca tgtaattttt
    12181 tctcagaaac aaaccactca ttggaaaaga catgaatcaa atcatcactt acttgtgtaa
    12241 aatagcgacg tttaatgaca aagatatctt cattgctatc tagtgtacct tcatttcttt
    12301 gtgcttaaat tcaaagataa ttttctcaat aaagattttt gattccagta agattccttt
    12361 actcctttta caacatagcg ttgtcctttg ttttgaaaca tcttcttccc ctctttgcca
    12421 ttaaaaatag cccaattctc tataaagaag attaagcttc attattataa tataaagttt
    12481 aaatttggtt tattacttac tttttcttat tctctagtaa gctaatattg aaaaaaatgt
    12541 aatcagatca ttatttttat atgttgtaac ttcctgataa gaaaactaat taaaagaaag
    12601 aggtaactac ataaactaat taaaaaagct aacttcatag ttggaggata aatggaagaa
    12661 acttcaatga ttgtttctcc ttgtgttcct tcaatcgaca ctagtcctat tatcaatctc
    12721 tcttttttgt ccatttactt ttgcgactgt tccccaataa tcaaatacag gaagcgttat
    12781 tttttgtcct ttagattcat gtactgtata gactattttc gaatccatca cttgataagg
    12841 catagtatgc cattccttgc caattcttac ttcatgattc tgcaaatgtt ctttgtattg
    12901 taatgcttcc gtgggtgcat agtcataaag tcctccggga aggagtgtcg tgtaacgaat
    12961 aagattttct tgattcatta ccaagtgatc tctctgagtg acaactttat gttcatagtt
    13021 tgcttttgtt gcagcagata cagccaacag taataaaaca attgccgtta tattaactac
    13081 ttttctttgc ttagctgatg tgctcttcat gaaaacgaac gataaaatca acgagccaaa
    13141 cagtacttga aagcctaaga ttcgccaagg aaattgaatc atttgaatgg gtgtattttg
    13201 taataaagac catggaaaaa atttggtgga taagagccaa gagactaccg cactaaggta
    13261 aatcactttt tctttgcgat taaatccttt aaaaaagaag agtggacaga tcaaagaaat
    13321 aataccaatg attcctaccg taaaggttgt caggtcatta ttcatcgcac caatcaaact
    13381 atccgagaca tttaatgctt ggtgttgtaa ggtcgtttta tctggctcat taatcgattg
    13441 aaccatgatt tgtcggaaca tcggatacca aaagtatgct gttaaaagaa tcgtcattcc
    13501 tactgccttt gacaactcca caatcaaccg gatgctttct ttttttcttg tttcacttac
    13561 tacaaagccg cctaagacta gtaagccaac ggttattaca ctcataaaag tggaaagaac
    13621 atgtgtataa atgagtaagc tcatactgac tgctaatgga attgcacatg gctttttctc
    13681 ataaatcagt gcgtaagcat aatacataac tatcggtata accgtaagtg caatcgcttc
    13741 agcaatagca gaacgatgat aaatatcaat cgtacggtac ccataaaatg tatataaaca
    13801 gctaaatata aaagccgcat cgattctttt taaaaatttt agtccataat gaaacatcag
    13861 catgatagta caaacattca atagccaaac atataagata taactaacga ttaaattatt
    13921 acttatccaa taaaaaatga ctgccggaaa aaaggtcaag tagggataaa aataattcac
    13981 tccgctccca taatgattaa acgtcgtaaa attgattgga ccagaaaaaa ttgaagataa
    14041 ccctttgact ctagcaaaat ggaaggcaat atcccccgat gtagaaatag tatgaacatt
    14101 tctaaaataa ttgattgaat aaagtccagc aataattgct aggagaatgg acaatattat
    14161 ttttttcttt acttgcatta cttaattata tactcctttc atggattgct ttatctagta
    14221 taacttaaat tttaaataaa tcgttattta tttttactct cacttaaaaa agcactaaaa
    14281 ttataagaga gggaggtaat ggtctgaaca ccaagaattg ttccaacatc gaattgctta
    14341 actaatgagt tcaaagctta attatgtggc tcttatattt accgagcgtg gattgcgatc
    14401 tctttagcga aacaaacatc tttcataaaa aaaacagcca tatccgcttt cttggatatg
    14461 actatttact actactttac tatctttcat tcttcttgtc tcacttatga taaggcgctg
    14521 ggtatcatga gtgaatgata ttgctgaata caaatagtcc gaccacatta agttgatcgg
    14581 acttcataag cattttattg actaatactt ttttgcttaa gaattgattc taacatcttt
    14641 tgctaaagtt aagcatccta tagttcctgc attatctgct aacttaggtg tgacaatgta
    14701 ttcttcgata tttggcgtat cgacatagcc gttaattagt tctaaaaaac gtctttgtac
    14761 tttctttttc agatgttctt gttgcatgac accaccacca aaaattataa tatctggtga
    14821 aagcatcagg gtcgtattac aggcacattg tgcaatataa tccgcctcga tttcccagaa
    14881 cggatcttct atagaaagat cttgtccctt ggttcccgta cgcatctcga tagcgggtcc
    14941 agatgccaac ccttcgagac aatcatgatg aaaaggacaa gtaccagaga aagtatctcc
    15001 tgaatgtctc ttaactaacg tatgccccat ttctggatgg ctaaaccctt caacaaaaat
    15061 cccatcttgt atagctcctc caccaattcc agttccaaca gtgtaataaa caacactgga
    15121 taaacctttt ccatgacctg aaacatactc cccgtaacaa gcagcattca catcggttgt
    15181 ccaggcaatt ggaatttcga aatgcttatt caacgttcca acaaaatcaa aatttttcca
    15241 ggcagttttt ggagtagagg tgatatgtcc ataggtcttt gattcacgat ggatatcaat
    15301 cggtccaaat gaccctactc caatgctttc taattgttct ttatagtgat taaagaaatc
    15361 aattaccaaa ctcattgttt cattcggtgt agttgtagga aaactcactc tttctaaaac
    15421 ctttaaatca tcattgccaa tggcacaaac aaatttagtc ccacctgctt ctattgagcc
    15481 atacatttct tactccaccg tccttttatc taggaattcg ttcttccctg tctaatagtt
    15541 tttcgaaaaa ctcatgtggt tccttttggt accaataggc aacagaagaa atatcgtctt
    15601 gacgctcata aagcttgatg tcatcatttc ctagggtttg gaaagtaaca cgtaaatttt
    15661 ctttgaaact aatggggtcg ggtaaatgcc agcggtacaa cccatgcatc ggtaaagcat
    15721 catcgccaaa accatgaact ggatttggat taggttttcc agcactaaaa taatcccggg
    15781 tgtgatctct tttagattga aaaggatagc ccataaatag cgtattaaaa ttttgagcat
    15841 atgccctttc tccaaagttc tttttatgga aagcccaagc gccgccaaaa taatcttctg
    15901 ctcctgtaga tgttattgta gggaaatctt catctccatc tatataaaac ttaaattctc
    15961 cttcccccca ccagtatctt tccaatgcgc acaaagcaaa gtaggttcct acataatacc
    16021 cagtgccttt gattccatca ataagggtgt aatcttttcc ttttgtcgtg atgggttcac
    16081 ggttccattt agcatgaaaa tacatcgcat catccggtac ttcagtcaat gcataattaa
    16141 atgtataaaa aatgtgctta ataaactcag gatgttcatt cgttaaagta atcttagctt
    16201 tttttcgaaa tggcatctca aaatagcaat tcatccctga tgttggattc actacgattg
    16261 gtaaggaatt cacttcgcat cttgtcccaa acccattaca aaaaaaatct cctaatggac
    16321 tttctacaga aggagtggat tcatcatccc aataaatacg caaaattaaa tcacgtaaga
    16381 cgaaactccc agcgattgtt ttgtctgtca ctgtcatcca aaaatgacgt aaaattccag
    16441 ggccttccat ttctgctaaa gtaattgtct ctccagcatt tatcggaaca gaaggtgacc
    16501 cttttctccc aattcctaga tgactcgctg tcattgccgc tttccctttt tcaccatttt
    16561 tattttctgg tgaaatagtt ctactttcct ctcccttgtg gagccatata ttatttaaac
    16621 aattcatttt attcgtcctc tatcctttct tcactttttt gattattcat atagatgatt
    16681 tgttgttgtg cctccgcaat aattgctcca atatctaaat tatcatttcc taactgcaaa
    16741 gcgattgaca ttgccaaggg aagattcatt cctgcaatta cgtgcactct gtcagaaata
    16801 taggtaaaaa acttttgatt tacacttcct ccagccatgt cagttaaaac aattatttcg
    16861 tctgtagtat ccactaactc tttaatttgt tcatctatat catcaatttc ttcgacatat
    16921 gcacataagc tactaatttg attatgcata cctgttaaaa actctagtgt ctctttaaaa
    16981 ccctcagcta gtattgaatg gctagcaatg atcaatcttc tcataattca aatccttttc
    17041 ctttacgcta atattccaaa tgcagccata accatcgcaa aaacaataac gagtagaatc
    17101 aaaatggtca tactgatttt tttcttactg agtagataat atgctgcagc agtaataatt
    17161 actggaatta atgttggtaa aattttgtcc aacattggtt ggattttcat actaacttca
    17221 ccaaaagtga atttaagtgg cgttgaaaca ctgataactg aagcaattaa tgatccaact
    17281 actgttagcc ccaatactga agcagcttca gttaaataat taatcttttc accgtattca
    17341 gtaacaatct tcattccctg cttataacca aattcaaagc ctctcattcg gaaaaagaag
    17401 attaccatga ttaaaatgaa ccataaaaag attcctacag gattattttc taaagccata
    17461 taagccgcaa ttgatcccat gatggttggg atcatcgtca tcaaaagaga gtccccaata
    17521 ccagcaattg gacccatcgt accaacttta aagtcattaa tcgcatcttt tgcttctaat
    17581 ccttgctttt cctccattgc aacacaagca cctaaaataa tggctgccag ccaaggctgt
    17641 gtattgtaat actgaaagtg attatttaaa gcttcgatgt attcatcatc atctttgtat
    17701 aattttctta aaataggtga caatgcgtaa gtcacagatg caccttgctg tgtttgataa
    17761 ttaaaggtac aaactgacat caaccaacga tagttcgctt tcctaagatc ctttttcgtt
    17821 acttgatatt tttcgctcat tttattcatc ctccccaata aacccaacat cagaagctcc
    17881 cgcagaagca gtgatttgtg catctttttc agctctatga tacacattaa atgctaaagc
    17941 gaatcctatg atcgccgctc caataatagg taccttcaaa aaagcggcta agaaaaatcc
    18001 aacaagtaaa taactaatgt atcgacctgt aggcataaag cgtaacaaca tcgcaatacc
    18061 aacagcaggc aacatttttc cggctaatgt tagcccatcc ataaaccaat taggtaaaac
    18121 atccaaaacg taattaacag cagactgtcc taaagtaacg gcaataaata ctggaattgc
    18181 tgcagataaa ccaaagaaaa ttggccccaa ccacaaaaca ctattcattt tcttaaattc
    18241 tcgtttattc gcatattcct gtgatttgcg aacaacaaaa ccatttaaga ttttgacaat
    18301 tacatctagt tgcaccccta acatacctac aggtagtcct attgctaatc cggcttctac
    18361 tccaagctgg ctaccaaatg ccgttgcaat aattgcgcac gttccatagt ccggtacaga
    18421 tgagccacca aaaccggcaa ctcctaatga catcaattga actgtaccgc caactatcaa
    18481 acctgttttt atatcaccca tcaaaattcc tgtgaataat ccccaaaaaa ctgcgtaatt
    18541 tacaaagatc atcggaatcc cataataatc aacatgttta ataaatgcta atatagtcag
    18601 cataataatt tgaaaaatag taaaatgcat tatccttact cctttatcct aattttgatt
    18661 gaatttcctg tgccttatcc gccggaacaa attgtgtaac aatttctaca ccagaatcca
    18721 acaatttttt atacgtgctg atattttcat ctgatgcata ggctctagaa gatagctgta
    18781 atgcattatc tttaacaaca gtaccgccaa caattagtcg gtcaattcta ataccgttat
    18841 ctactaaatg aaggattgtt tccggctctt tcacgatgac aaatactttt tcctgatcgt
    18901 atttcccctt tttaaaattc gcaatcgctg tttcttcagt gataattgat acagccattc
    18961 cagcaggtct agctaaacgc atacttgatt tttggatgtc gttatttgcg acagaatcat
    19021 cgatcaccat cactctgttt gctttagaca ttggtgccca ttgcgtgaca ataatcccat
    19081 gcaataaacg gttatcaact ctcgacataa caatactcat aaatacttcc tcctttatct
    19141 ttcattttct tacaagcaat taccatgcca aaacaaaaag aacttgataa atcaagctct
    19201 ttttacataa tcataaacgt atttaacctc atgaatcggg atgtcgacac tataataatg
    19261 ttcaacgaca ctgaaagatt gcttaaaatg tgtctgaaat ttttgaaatt cctcactcgt
    19321 ttcattgaaa taaatttcat cttccaaagt attttgtttt actaatcgtt caattaagca
    19381 actaatgtga acatataatc cgaaacacgt attattatca aatgaa
//
LOCUS       DAYYCW010000054        19338 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000054 DAYYCW010000000
VERSION     DAYYCW010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.054"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..656
                     /locus_tag="ACHAYA_001685"
     CDS             90..656
                     /locus_tag="ACHAYA_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8605483.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            801..888
                     /locus_tag="ACHAYA_001686"
     tRNA            801..888
                     /locus_tag="ACHAYA_001686"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:833..835,aa:Ser,seq:cga)
     gene            1224..2198
                     /gene="bsh"
                     /locus_tag="ACHAYA_001687"
     CDS             1224..2198
                     /gene="bsh"
                     /locus_tag="ACHAYA_001687"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8605484.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            2523..4418
                     /gene="asnB"
                     /locus_tag="ACHAYA_001688"
     CDS             2523..4418
                     /gene="asnB"
                     /locus_tag="ACHAYA_001688"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM8605485.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(4613..5059)
                     /locus_tag="ACHAYA_001689"
     CDS             complement(4613..5059)
                     /locus_tag="ACHAYA_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8605486.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            5287..5436
                     /gene="rpmG"
                     /locus_tag="ACHAYA_001690"
     CDS             5287..5436
                     /gene="rpmG"
                     /locus_tag="ACHAYA_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8605487.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            5524..6042
                     /locus_tag="ACHAYA_001691"
     CDS             5524..6042
                     /locus_tag="ACHAYA_001691"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8605488.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(6119..7075)
                     /locus_tag="ACHAYA_001692"
     CDS             complement(6119..7075)
                     /locus_tag="ACHAYA_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8605489.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            7241..8047
                     /locus_tag="ACHAYA_001693"
     CDS             7241..8047
                     /locus_tag="ACHAYA_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8605490.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8044..9033
                     /locus_tag="ACHAYA_001694"
     CDS             8044..9033
                     /locus_tag="ACHAYA_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8605491.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            9030..10034
                     /locus_tag="ACHAYA_001695"
     CDS             9030..10034
                     /locus_tag="ACHAYA_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8605492.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            10166..10708
                     /locus_tag="ACHAYA_001696"
     CDS             10166..10708
                     /locus_tag="ACHAYA_001696"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM8605493.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            10728..11426
                     /locus_tag="ACHAYA_001697"
     CDS             10728..11426
                     /locus_tag="ACHAYA_001697"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM8605494.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            11447..11659
                     /locus_tag="ACHAYA_001698"
     CDS             11447..11659
                     /locus_tag="ACHAYA_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM8605495.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            11659..12621
                     /locus_tag="ACHAYA_001699"
     CDS             11659..12621
                     /locus_tag="ACHAYA_001699"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM8605496.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            12639..13034
                     /locus_tag="ACHAYA_001700"
     CDS             12639..13034
                     /locus_tag="ACHAYA_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8605497.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            13197..13562
                     /locus_tag="ACHAYA_001701"
     CDS             13197..13562
                     /locus_tag="ACHAYA_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM8605498.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            13559..15370
                     /locus_tag="ACHAYA_001702"
     CDS             13559..15370
                     /locus_tag="ACHAYA_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM8605499.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(15432..15599)
                     /locus_tag="ACHAYA_001703"
     CDS             complement(15432..15599)
                     /locus_tag="ACHAYA_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM8605500.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            15848..16837
                     /gene="galE"
                     /locus_tag="ACHAYA_001704"
     CDS             15848..16837
                     /gene="galE"
                     /locus_tag="ACHAYA_001704"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM8605501.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            16849..18333
                     /gene="galT"
                     /locus_tag="ACHAYA_001705"
     CDS             16849..18333
                     /gene="galT"
                     /locus_tag="ACHAYA_001705"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM8605502.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            18357..19337
                     /locus_tag="ACHAYA_001706"
     CDS             18357..19337
                     /locus_tag="ACHAYA_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8605503.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         6119 a         3296 c         4068 g         5855 t
ORIGIN      
        1 aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata gggtacaata aaacaacata
       61 tactgcagta aagaagtggg ggagttttaa tgagaagaaa agtctatacc aaggatcaaa
      121 ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt taaaggattt actgcgagaa
      181 atattgctaa aaagatgggg atttcgacac agccgattta tctggaattc aaaaacatgg
      241 aagatttaaa aaatacgttg ctggattcta tctttgaaca actaaaagaa gatgttttcc
      301 ctattgaacg tactggtgat aaaatcattg atctcggttt gaactatatc tactttgcaa
      361 aaaagcatca tagtctttac atggcattgt acattgatga acatggggac ggaaagaaaa
      421 tgcatgattt ttcatacaat tatttccgtg aaatagttaa agaagatgaa agatacaatc
      481 aattatcgga tgaatatgtc gatgctctgc atactggtac atgggtagca gtgactggct
      541 tagcgtcatt gatgtcatca aacatcatgc atccaacaga agaacaaatc attgcactga
      601 tcaaacggac aattgactcg attttagcat tagataatcc tgaaataatc agatagtgtt
      661 ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa actgtcaatg gacttttttg
      721 ctaaaaaaag cgatttctgt tatttatcta ttagaaatag gcttgacatt tcgtgaaaaa
      781 agtttaaaaa tagtacttac ggaaggttgg cagagtggta atgcaccgga ctcgaaatcc
      841 ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt accttcctta tatcaacgat
      901 tagaggttgt gaagaagctg tcctgcatca agtaataaga acggccaaac aaaaatagct
      961 tttcctattt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
     1021 tgttcggaat aaggggctgt gacaagactt ttgtcacagc cccttattgt tgtactttta
     1081 acctacaata ttttttgatt ccccctgagt actatttttt ataaatgcta gtaaatcaat
     1141 cttctttggc cttttttttg ataaaaaaaa atactagatg taagataaaa gtgtaaaaac
     1201 gatttgtttt aggagggaaa acgatgtgta ccgcaatcac ttatgcaacg aaagaccatt
     1261 attttggaag gaattttgat tatgagatgt cttataatga agtagtcact attactccaa
     1321 gaaattatcg tttcgatttt agaaaggtaa aaaatttaga taaacattat gcaatgatag
     1381 gaatagcagc tggggtatcc aactatccac tctattatga ggctacaaat gaaaaagggt
     1441 tgagtatggc aggactgaat tttcctggca atgctgatta taaagaatta caagaaggaa
     1501 aagataacgt tgcaccattt gagtttattc catggatact agggcaatgc tcaaccatag
     1561 acgaagctaa agaattattg gctactatca atctagtcaa tatcgatttt agtgaaaaac
     1621 tacccttgtc acctttacac tggttattag ctgataaaga aaaatcgata gtaattgaaa
     1681 gtatgaaaga tggacttcat ttatatgata atcctgtcgg tgtgttgaca aataatccgc
     1741 catttgatta tcaattattt aatctgaaca attatcgctc gctatcaaat ggaacaccag
     1801 aaaatcattt ttcaaaccag atcagcttga atgtttatag tcgtgggatg ggaggattgg
     1861 gcttacctgg agatctctct tctgtttctc gatttgtaaa agccacattt actaaaatga
     1921 atgctgcatc aggtgattca gaatctgaaa gtatcagtca attttttcat atactaggct
     1981 cagtcgagca acaaaaagga gtatgcgata ctggagaagg aaaatacgaa tatacgattt
     2041 attcttcttg ctgtaacgtt gataaaggga tttattatta tcgtacgtat gaagacagtc
     2101 aaattactgc tgtaaatata aataaagaag acttggatcg tcatgaattg gtcagttatc
     2161 cagtcataaa agagcaacag ataaactatg tcaattaatt aatgaataga tccgtttggt
     2221 atcatatttt ctgatacagt cataattaga cacgatcttt aggtcgtgtc tttttttgct
     2281 gttttttttg gaaatagcat ccactaatat ttttttaaca cgaagagaat aaggtagcct
     2341 caaaaaagaa aacatggtaa attagatatt gggactttat tttctgtaaa aacagaaact
     2401 ttcatctttt gttttcacgt gacgaaattg taagagagaa aatattcgtc cgctatttat
     2461 tctatgataa gatgcatatg aatgaaataa acagtaattt tatattagga tgtgaaagtt
     2521 aaatgtgcgg tattgtaggt tttgttaacg acaaggacaa caaaaaaaca attattaata
     2581 caatgatgga tcgaatcgtt caccgtggac ctaatagttc aggagaatat atcgataaac
     2641 atgttgcttt aggattcaga agattaagta ttattgactt agagggcggt actcagccta
     2701 tttacaatga agatcggaca aagatcatta tttttaatgg cgaaatctat aattaccagc
     2761 ctttgagaga agaattgatt gcagctggcc acgtttttca aacacatgca gatacagaag
     2821 tccttttgca tggttacgaa gaatggggaa cagaattatt gcaaaagatc cgcggaatgt
     2881 ttgcttttgc tatttgggat aatgaaaaga atgagttatt cggtgcaaga gaccactttg
     2941 ggatcaaacc atattattat gcagaaatga acggaacatt tatgtttggt tcagaaatca
     3001 aaagttttct gcctcatcct gattttaaca aagaattgaa caaagaagcg ttgaaacctt
     3061 atatgacttt ccaatattct ccattaaacg gcgaaacatt cttcaaagat gtctatcgtt
     3121 tgcctgaagg ccattattat acgtataaag atggaaaact agacattcag caatattggg
     3181 atgcagattt tgaaacaaaa gaaacgcatt cccgacaaga atggatcgaa aaaatcgacg
     3241 aaaccgttca agcttctatt gaagcacata cggtcagtga tgtagaagtt ggttctttct
     3301 tatccagcgg ggttgattcc agttatgtaa cctctgtatt gaaaccagat cattctttct
     3361 caatcgggtt tgatgataaa acctataatg aagcgattga agccagaaaa ctaaccgaac
     3421 tattggactt agataataca gcagcagtga tcgacggaga catgtcgttc aaagcattcc
     3481 ctttgatcca gtatcactta gatgaacctg attcaaatcc ttcttgcgta cctttgtact
     3541 ttttagcaaa tcttgcttca caaagtgtgc gggtagtcca atctggagaa ggggcggatg
     3601 aactgtttgc gggataccaa acgtatggat tccatacgaa ttcaaaattc atccgtgtga
     3661 ttgctcaagg attgaaaaaa ttaccaaaag gaacacgcta taatttagga cgtaaaatcg
     3721 gaaaaatgaa aaatttccat ggacggatcc atttatatga atcacttgct cctgcaaaag
     3781 aatattttat cggtcatgct cgagtgttcg aagaatcaga agcatcagaa gtgttaacac
     3841 caaaatatca aacagcacca tctgtggatg aaatcatgac tgttcattac gagaaaacag
     3901 aaggaatcaa agatgaagtc aataagatgc aatatgtcga tcttcatcaa tggatgccaa
     3961 aagatatctt gttaaaggca gataaacttt ctatggcaag ttctttggaa gtccgagtac
     4021 ctttattgga tatcgaagtc atgaaattgg ctcaacaaat accaagtaag tttttattga
     4081 atcaaaacaa cacaaaagat attttcagac aagctgcgaa caaacatctt ccggaagaat
     4141 ggtcgaatcg tgtgaaatta ggattccctg tgccaatcaa agcttggttg aaagaagaac
     4201 acggctacga acaagtcaaa gcattattcg aagcagattt tgcaaaagaa ttttttgatc
     4261 aagaaaaaat catgaagttg ctagatgacc atcacgaagg acgtaaagaa gagcaaagaa
     4321 aaatctggac aatcttcagt ttcttgacat ggtataaagt ctactttgta gatgaaacga
     4381 ttccgcaagc agaagcaatc gactatgtga ctgtttgatg aaaatcaaat atcaataaaa
     4441 aagaggtttt ctcctaccgt ttaacactga gtgataagaa acaatttcaa aaaacagctc
     4501 ttcccgtttt atagatatta tgacttatgg ttcggagttg gcagaggaac accattgttt
     4561 cgaacataag ggggttgtga cacgattttc gtcacaaccc ctttacatat gttcaataat
     4621 cgatttcttt ttcttttgtc ccatcttttt tataaatgaa caaagttgat tctttgtttt
     4681 gtgctacttg ctttcctttt tcgattgctt ctttttttgt atcaaaatgg ttgcttgcct
     4741 tttttgcccc tttagatttc acgatccatt gttcttcctc atattctacg atcacatctg
     4801 aatcaagcag ttttccagca cgaggattcg tatcgtgttc atccgtcttg cttggatttt
     4861 tttctttttc gaaagttcgt ttttctgatt cactagcatc ttcataccat ttttttgctt
     4921 gatcaatggc aattgggatc gcacggttat cagggtaccc ttcatctaat aatgcatttg
     4981 cgatgtcgat tgcttttttt cttgtcaatt tatctaagtt tttcattgat gcaggaaagt
     5041 ccttcatgtt ccatggcata ttagtatccc cctttattaa aaaacataat ctatataaac
     5101 aagtgtaagg gtatttatac aaaaagaaaa agattttgtt tgagaagtaa aaccgttgta
     5161 tttatcgtca agtagtttta cttaacaaaa tcgtgaaaaa cactagcaat ctagaaaaaa
     5221 tcatggtaag ataatcaagt ctgagagtca tcttagagga ttcttgtaaa aatgggaggg
     5281 aaaaacatgc gcgtaaacat cactttagag tgtacttctt gtaaagaacg taactatcta
     5341 acaagcaaaa acaaacgtaa caatcctgat cgtttggaaa aacaaaaata ttgcccacgc
     5401 gaacgcaaag ttactttgca ccgtgaaaca aaataatgtt tttaaagttc tcaattcctt
     5461 gctggtcaaa gaattgagac tttttttatc tcaaaacatc taaaatcata aggagcggat
     5521 aatatgttta tttccatgtg ggcacaagat aaaaatggtc tgatcggcaa aaatggtttg
     5581 ttaccatggc gattgccaaa tgatatgcgt ttctttcgcg aacatacaat ggatcgaatt
     5641 ttagtgatgg gaagaaagac ctatgaagga atgggggatc tttcacttcc ttatcgccat
     5701 atcatcgtgt tgacaacgca accaaatttt cgcacgaaag aaaatgcgga agtaatgcat
     5761 tcgatcgatg aacttttgtc gtatagtcaa acgatctcag aagatatcta tgtttcgggt
     5821 ggtagtcgga tttttcaaga acttcttcct tacactggtc ggatctggcg aacattgatc
     5881 gatagcacgt ttgaaggcga tacgtatatt gggaatattg atttttcaga cttcgccctt
     5941 atagaggagt atgaggggat aacggatcaa gaaaatctat atgcccatcg ttttcaaaaa
     6001 tgggaaagag taaaaaatag ggttgcttca aaagaagaat aaaatctatg cgtctagttt
     6061 gttaataaga aaaaaagcat ctcacgacta atcgtgaaga ttcttttttt agttaaaatc
     6121 aatttagttt ttctttgata tcattgacca tttcctgact ggcaataggt ccgttatata
     6181 gccaactaga ttcaggtccg aattgcagta catgattgtc tgtcactgct ttaagattct
     6241 tccagcttgg ttcattgaac atggcagcat ctttgtcact gttcacaagg atgatgtagt
     6301 cagcatctaa ttcagctagc ttttcagagg aaacagcaga ccagtcagaa gtggcttttt
     6361 tggagacctc ttccactaga tcaggaacac caaattccag atcgtcgtat acgattcttc
     6421 cacttgaccg atttttgctg accataaagg ctgagttgtt ggtcacccac aacactgcag
     6481 ctgtcttgtt ctcgatttta tctgctagtt cttctcgagt tttcttgatg gatttttgat
     6541 aatcggaaat gattttttct gcctcttttt cttttgccaa agctttgccg acatctttta
     6601 gttgttcttc ccatgtgaca tctgttccgt tttttacaac ataagttggc gcgattttgc
     6661 tatattgttc gtatttcccg ccttcgacag tagcggaaga agaaatcaac agcaaatcag
     6721 gctcaaagct taacactttc tcataaggaa ggtcataaga aatcgttggg acatccttta
     6781 aatcatcttg cagataatct tgaatggaac cactgccaac agtccactgg gcgataggtt
     6841 tttcccctag tgcaatcaga tagtcttcta agtaactgcc gatgatccgt ttgggcgcgt
     6901 ttggaatctc tactttatgt ccaagtgtat ctgtgactgt atgagtacta ttggatgaag
     6961 tcgtcgaatt ggcagttgta gctgtttctt tatctgaaga gggggagcaa gcgctcagta
     7021 ctccagccag cagcaatatg ctagataatg tgaatacttt tgtttttaat ttcatttctt
     7081 tcctcctaac ataattgaga atctttctca ttggttatta ttcctgatat ttcgaacgct
     7141 gtcaaccgct tcatttggct atagatatga aagtagcgct tgacgaaatg ttgaaacagc
     7201 gtcataattg agaatgattt tcagcgaaag gatgggtgaa atgacgagat taagagcagc
     7261 tgacattgca gccggctacg aaaacaaaaa aatcattgaa aacatgtctg ttacgattcc
     7321 tgaagggaaa atcactagtt tgattgggcc aaatggaagt ggaaaatcta ctttattgaa
     7381 aagttttacc cgtatactac ctgtgaaatc aggaaagatc attgtcaata actctgatat
     7441 ctctacatac agttcaaaag aacttgcaaa aaaaattaca ttattagcac aaactagtga
     7501 acaccctaca ggtatgacag tagaagaagt agtatcttac ggacgatacc cttatcaaaa
     7561 actgttcagt ggcatggaca cgcaagatca tgaagcaatc gagtgggcgt tggatgctac
     7621 aggtctagct ggatttcgta tgcgggatat cgcaacgctg tctggtggac aaacccagcg
     7681 tgtctggatc gctatggcgc ttgctcagga aacagatatc ttgatcttgg atgaaccgac
     7741 aacattttta gatccggcac accaattaga gatcctacag ttattgactg aaatcaatca
     7801 aatgaaacaa acaacgatct tgatgtcgat tcatgacatt aatcatgctt cacgtttttc
     7861 agattattta ttagcaatga aaaacggtaa gttgatcaca gaaggtacac ctgaagaagt
     7921 gatcacaaaa gagtgtatga aaaaaatatt tgagattgat gcaacgattg tcacgcttcc
     7981 aagaacaaat aaaccagttt ttctaaccta tgatttgttg ccaaaagagc aggaggatca
     8041 attatgagga ctatgaagaa aaatagactc ctgcttttgg tctgttttgt tcttttgatt
     8101 attttttttg ttgctctttc aaatggcgca gcgactgtgg gaaaagcaga tttttggcag
     8161 gcgttgtttg attttgatag atcaaaacaa agtcaccaaa tcatacgaaa tattcgtttg
     8221 cctcgtgtgg cgggagcatt tcttgtaggg agttgttttg cattgagcgg tgcattgatg
     8281 caaggagtga caagaaatcc tttagcagat tcaggcttac taggaatcaa cgcgggtgct
     8341 tcgttggcaa tggcccttag ttttgctttt tggcctaaag cttctgattt ttctgtgttt
     8401 ctaatgtcat tggcaggttc ttttgctgta actttgctcg tatttggttt tttgcagttt
     8461 tcgaaaatgg gcatgaatcc tttacagctg attttagctg gcgtagctat tagttctttt
     8521 tttactgcta tcagccaagc acttacccaa ttctttaatc tgcagcagga tcttgctttt
     8581 tggtttgtag gcggagctgc caatatcaca tggaaacaat tagcagtatt gtttccttta
     8641 tatatacttg ctgttttggt ttctttttca ttaggaaaag caatatcttt gattcatttg
     8701 agtgaaacac atgcattagc tcttggaagt catcctaaac gaacgcggtt gattgttttc
     8761 tgtctagttg cagtgttagc agccattgcc gtttcgttag tcggacctgt ttcatttatt
     8821 gggctgttgg ttccgccagt tgttcgcgga atggttggaa cggactatcg ttatgttcta
     8881 cctgtctctt ttttagttgg cggtggattg gttatgttgg cggattttac agcgaggatg
     8941 atcaatcccc cttttgaaac accttttgga ttagtcattg cattgattgg cgttccgttt
     9001 ttactgatac aagtaaggag ggaacaacgg tgaaaaaaat atattggacg ttctatttct
     9061 tgctttttgt acttgtcatc ctgtctatag gtagtttaat gattggtacg ccttttattt
     9121 acctggatca gctttttgcc acgttcatag ggaaagaatc atcttctttg gcgctgattg
     9181 ttagagaatt tcgagttcct cgattactga tcagtttatt agcaggagct tgtcttggcg
     9241 tttctggtta tatcttgcaa ggagtaacaa gaaatgaatt ggcagattct agtatattgg
     9301 gcattaatgc cggagcagga tttttagtga tgctttattt aggtttcttt tcgcactatt
     9361 cgctgccgtt tctattgcta gttgtagcct ttctaggtgg tgttttagct gcttgctgtg
     9421 tctatcttgc ggcctatgat cgaaatggct ttttgggtat gaatcgaatc ttattgtccg
     9481 gtattgccgt taatgccggc ctttctgcat taacattgct atgcacgatc caattatcta
     9541 aggaaaatta tggatttgtg aatgcatggt tagcgggatc aatctggggt gcgagctggc
     9601 cttatgtttg ggcattgctt ccatgggcgg ttatcttaat tccgctagga ggactttatg
     9661 cacagaggat tggattgcta tcatttggtc aagaacgcgc gcaaggatta ggccttaacg
     9721 tggctaaaag tcaaaaaatc cttttgttgt tagctgttgc tttggcaagc ggtagtgtag
     9781 cggtgacagg tagtttatct tttgtaggac tactagctcc tcatgctgcg aagctggtcg
     9841 tacgtaaaga aaataactgg acctttgttc tgagcggttt gttcggttcc atttttctgc
     9901 ttagtgcaga tatggttgct agagtgattc taccaagtgg ggaagtacct acaggaattt
     9961 tgattgccct ttttggtgcg ccatattttc tgtatgcact ttttatcaga caaaaacacg
    10021 cgctgaacag ttgaaattta ttaaaaaagg aaggccaatc atttctaggt cttcttttta
    10081 ttctatttat gcatttattt gaaagttcga ttgctatttt tctttttttc ctataccatt
    10141 ttatgagaga aggaagggtg agtttttgga ccaattgaaa gtaagattac gccggaaagg
    10201 aatcgaaaag ctgaaaaatc tggccaaaag tggcaagaaa aaggaaaaag aacagcgaat
    10261 ctataaacag ctattcgcca gcgagtactg gaagtcagca aaagtgatag ggacgacttt
    10321 atcgaatgaa tttgaactgg atacacagcc ggttatccaa cgagcagaaa aagaacaaaa
    10381 gaaaatagtt gtacctaaaa ctttgccgaa acgtcagatg gcgttttatg aaatggatga
    10441 acagtccgtt cttgtacgtt cgagttttgg tgttcttgag ccacaatcag agaagctcta
    10501 tttgccggag atgatcgatc tcctcattgt accaggtatt atctacaata gagccggtta
    10561 tcgaatcggt tttggcggtg gttattacga ccggtattta aataattttc ataaaaaaac
    10621 atgtagcctt gtttttcgtg aacaattgaa tgaacaatgg caaccggaga gctttgatca
    10681 gaagatccaa caattgttta ttgactaaaa tgaaatggga ggttttcatg aattatcaac
    10741 agcaaataaa attgcgtgca tggttaagac gaccgttatg gacctatgct tttttaggga
    10801 tacagacagc ggtcttcctc atgatggaat tatttccgcg acttgagatc ccttattatg
    10861 ccggaatgta tggtccttat cttgttcact tcaatgaatg gtggcgttta gtgacaccaa
    10921 tcttcatcca ttttggactg atgcatttcg taatgaactc actgatactg tattttatgg
    10981 ggcagcaaat cgaggcaatc tatggccatt ggcgtttttt cttgatttat atgttcagcg
    11041 ggataatggg aaatacagca agctttgctt ttaacgaagc gaatgttctg tctggtggtg
    11101 cgagtacttc gatattcggt ttgttcggtg cgttgtttat cttagggttt cattttaaac
    11161 ataacccagc gatccagcag ctagtcagac attttctttt atttattgtg ttgacatttg
    11221 tttttggtct tctggatacc tctgtagatg tttggggaca tgtcggtggg ctaattggcg
    11281 gattagtgtt agggaatgtt ttgggtctac cgaaacaaca gacaagctat tcgatacacc
    11341 aaaggattct ttctacacta gtttttgtat ttctttttgt aatatgtatt ttactgggtt
    11401 taaaaaaata tggtttactt gtataatatg aaggaagtgc ttaaagatgg agagcttata
    11461 tgatgtacag cagctgttca aaaaatttgg catctttatc tatgtgggtg cacgcatcta
    11521 tgacattgaa ttaatgacta tcgaattgcg taatctgtat gatagccgtt tgattgatca
    11581 tgatacatac atgacagcgt ggcgcatatt aaaaagagag caccgaattg aagaaagccg
    11641 aaagaaagga aatttgtaat ggacaagaag attattggga ttgatttagg tggaacaaca
    11701 gcaaaatttg caattttaac accagaagga gagattcaac aaaaatggag catcgatacc
    11761 aatattttgg atgatggtaa gcatatcgtt cctgaaatca ttgaatcaat caaccatcgt
    11821 ttgaatttat ataatatgaa agccgaagac tttatcggaa tcggcatggg taccccagga
    11881 agcgtggaca gcgaagctgg aacggttatt ggtgcgtata acttaaactg gacagaagtt
    11941 caatttgtga aaaaactgat cgaggcagga acaggaatca aattcgctat cgataatgat
    12001 gcgaatgttg ctgctttagg tgaacgatgg aaaggtgctg gagaaaacga tccagacgtt
    12061 gtattcgtta ctctaggaac aggtgttggc ggtggtatcg ttgctggcgg aaatttgatt
    12121 cacggcgtga gcggagcagg cggagagatc ggccatatca ctgtggatcc tgaagggttc
    12181 gaatgtactt gcggtaaacg cggatgttta gaaactgtgt ctagcgctac tggtgtggta
    12241 cgtctaggtc gttacttggc agaagaatac gcaggagatt caaaactgaa agcaatgtta
    12301 gacaacggtg aagaagtaac aagtaaagat atctttgaaa tggctcaaga agacgatccg
    12361 tttgcattga tggttgttga tcgcgtatgc ttctacttag gtttagcttg cgccaacttg
    12421 gggaatacat tgaatccgtc aagtattgtg ttaggtggag gagtatcggc agcaggagaa
    12481 tttttacgta gccgtgtaga gaaatatttt aaagaattta ctttcccgca agtaagagaa
    12541 tcaacaaaaa tcaaattagc agagcttggt aatgaagctg gtgtcattgg tgctgcctca
    12601 ttagccttga aatttgctta aaaaaacgga ggcattacat ggttttatgg gtaattaata
    12661 tcattttgct attgattgtg cttgcaatca tcttttggga agtctatcta cgagtaatgg
    12721 ccaagcgttc agcaacgact ttgacagaag aagaatttcg agaaggaatg agaaaagcac
    12781 aagtaatcga tgttcgcgaa aaagatgtgt ttgatgctgg gcatatttta ggtgcaagaa
    12841 acatccctta cactgttttg aaagattcat taggatctat tcgaaaagac cagcctgtct
    12901 atatttacga tcaaaagaaa agcttgagca tccgtacagc aaataaatta cgtaaagcag
    12961 gttatcaaga gatttattta cttaagggtg gatatgacgg atggtctgga aaaatcaaaa
    13021 gtaaaaaagt ataaaacaga tgattgtatg acagctttaa aaaccgaatc aatcattaga
    13081 ttttgaccaa gatcacatga acggcgcaat ggatagttgt gccgttcatt ttttttattt
    13141 tgtgctatta tctacatata taagctaagc gaaaaggcag aaagaaggaa acaactatga
    13201 tcaaattaaa acgtgtttac cttgatgctg aaaaagagga tggctataga atactcgtgg
    13261 ataggttctg gccacgagga atttcaaaag aaaaagcgca gttagatctg tggttgaaag
    13321 aaattgcacc tagtaatgaa gcacgcaaaa cttttgacca tgtaccagaa cgatttgctt
    13381 catttgcaaa gaaataccag caagaattga ttttcgaaga agaaaagaag caagctattt
    13441 ttaaactcag tcaacttatg aaagaacatc cagtcattac ttttgtttat ggtgcaaaaa
    13501 atgaacatca gaaccatgct gttttattga aaaaatttat cgaaatggag gcatgcaaat
    13561 gaaaataggg atcattggtg ccggacctag aggattaagt atagcagaaa ggattctcca
    13621 taataatcag gacagacatt ctcttgaact ttttcttttc gatcccaatg gtcctggagg
    13681 tcgagtttgg cggttgaatc agccaccgga gttattaatg aattctgttt ctcaacaagt
    13741 gacgcttttt acagatgaaa cattgtccag tggaggattg gtaacacagg gacccaactt
    13801 gtttcaatgg agtaaaggac ctgctacaga ttatatcgaa cagacggtaa ccaaaaataa
    13861 gcaactaatt atagaagaaa tcaatcgtct ggaaaaagat cagcaatcta ctcgatgttt
    13921 atacggactc tatcaacgct ggtttttctc aaagttgcaa gaagaatttc cagcttctat
    13981 tcagtttgtt tattctttag taaaaaaagt cagcaaaaga aaagatggtt atcttttaca
    14041 gacagatcag caaagctatt ctatcgatca gcttgtttta gcaagcggtc actgggaaaa
    14101 tgatgagaca gaagaagaaa gagtgtgggt agactacgca aagaaaaatg atttactgta
    14161 tcagcttcct tctaatcctg cagatgtcga tctttcgaag ataccgccta aagaaaatgt
    14221 ctttttaaga ggtctaggct taagtttttt tgattatgtc ggattgttta cagtatcaag
    14281 agaagggcgc ttcgagaacg aaaaaggaaa acttgtctat catccatcag gcaaggaacc
    14341 gattgtatac agtggatcaa gaagggggct cccttattac cctcgaggaa gaaaccaaaa
    14401 gcaaggcgga tcaatggcat ggccacggtt actaacaaaa gaaaacctgg agcaatggca
    14461 tcagtcggga tttttgactg gcgaaacatt ttttgactat ctgaaaaaag acgttgagtt
    14521 gttttattac aagaaaatca tcgaagaata tcatttgccg attccaaaga aaaattttga
    14581 gcatgatttt ctctccctct cttcagagga gtgtctcgga aagcatccag cactaatccc
    14641 ttatagatgg gattggtcgt atttagaaat tcctcttacc tatcaggatg agtcctttgc
    14701 agaagctagc cgagcattta ttgaagggca gataaaagaa gcggaaaaag gcaattgcac
    14761 aggagcattt gcttcaacat ttgatgcatt aaaagactgg cgtgatccaa tccggcaggc
    14821 tattgaatgg cacgtttttt ctgcaaagga atacaaagaa atactatggg gatggtttac
    14881 tcatctaaat agctttttaa cgatcggtcc gccagtcatt cggacgaaag aattagcagc
    14941 actgatagat gcgggtattt ttcatttgtt cgaaccgcca tttgatattc agcaaaagca
    15001 caaccgctat gttatttctc tacaagataa agaaatcagc agtcgctatt tgattgaagc
    15061 tcgattgcca caaacaagct tttctcgttc aagtaatcct gtcataaaaa atttgaagga
    15121 aaacaaactt gttcgccctt tttcattttc agataataca ggaagttatc attcagaagc
    15181 tatagatgtt gagcgaggta cgaatcgcgt gattgatgct tcaggggaaa tccagtctga
    15241 cttttattgc tatggaatac ctgttgaagg tgtggattgg ctaactgcag cagtagctag
    15301 accttataca gatccatgga atctgagaca ggcagataaa atagcacaac ttatattgac
    15361 tgacaaataa aaatacgcat gatgacttat ctgttctttc acaagtcttc atgcgtgttt
    15421 ttaaataatt attaacgaga aacgcgtttg cgcattgctt ttttacggtt ttcttcgatc
    15481 attgcttctt tttcgtcaat tggatcgatc actgtttttt tcacagaagc gaccaaacca
    15541 gcaacggcag cggctgttgc cagacttcct actaatattc cagatacaaa ttttttcatt
    15601 gaatccacct catttattat ttgttatatc tattatggaa caaaagagaa aaaaaggcta
    15661 gttttacgtt tgtttgtaaa cctttgcaaa aaatccttga aaatgtttac taaatgtttt
    15721 attataaatt taccatatga atttacgaaa ttttactttg tttgttttca ccataaaagt
    15781 aaacttcgtt tttttaaatt atatataatc aacaaacaca attttactaa tcaaaggagt
    15841 gttttttatg acaattttag tactaggcgg agccggttat atcggttcgc acgctgtaga
    15901 ccagctagtt caaaaggggt atcaagtagc tgtggtagat aatttgctca caggtcacaa
    15961 acaagcagta catccagatg ctcattttta tgaaggagac atccgggata aagaatttct
    16021 acgttccgta tttgaaaagg aaccgattga aggagtaatc cattttgctg caagctcttt
    16081 agtaggagaa tctgtcgaaa aaccattgat gtatttcaac aacaacgtat acggtatgca
    16141 aatcttactt gaagtcatgc acgagttcaa tgtgaataaa atcgtgttct cttctactgc
    16201 agcaacttac ggagaaccaa aagagtctcc aatcacagaa gatacaccag ctaatcctaa
    16261 aaatccgtat ggtgaaagta aattgatgat ggaaaaaatg atgaagtggt gtgaccaagc
    16321 atatggcatg cgctatgtcg ctttgcgcta cttcaatgta gccggagcaa aagcagatgc
    16381 atctatcgga gaagatcata caccagaaac gcatcttgtc ccaatcattt tacaagtagc
    16441 tcttggtcaa agaaaagcat tggctgttta tggagacgat tatgatacac cagatggaac
    16501 atgtatcaga gattatgttc aagtagaaga tttgattgct gcacatattt tagctttgga
    16561 atacttgaaa gaaggaaatg aaagcaactt cttcaattta ggcagcagca aaggatactc
    16621 agtcaaggaa atgctagaag cagctagaga agtgacagga aaagaaattc cagcagaaat
    16681 agcaccaaga agagctggag atcctagccg attagtcgct tcaagtgaaa aagcgagaga
    16741 ggttctgggt tggaaacctg aatacacaga tatcaaagca attatcaaaa cagcatggga
    16801 ttggcatgtc agccatccaa atggttatga agaatagggg aatgagatat gtcaatcagt
    16861 caaacgattt gtgattttgc cacacttgcg atcgaagctg gcggatggat ggaaatggac
    16921 cgcttatatt tacagaatcg tcttttaggg atggtaggag aagatgctct agaaagcatt
    16981 gaagtaagac cagttaagga agaatctgta gacttattgg atcagcttgt cgctaaggca
    17041 agagaaaatg gtgttctttc agaattgcca gcagaacaag agatttttga agcgcaatta
    17101 atggattttc tcacaccgcc accttcagtc gtcaatgcat tctttgcaca gcactattcg
    17161 aaaagcccag aggaagcaac tgagtatttc tttaatttaa gtaagaaaaa tgactacatc
    17221 aaaacaagag ccatcgcaaa aaacatcaaa tttccggcag agtcggagta tggtgaactg
    17281 gaaatcacaa tcaatttatc taagccagaa aaagatccaa aacaaatcgc agcagaacgc
    17341 gaggcggtaa aagtagacta tcctacctgc atgctttgca tggaaaacga aggatataga
    17401 ggtcgcttga actatccagc ccgcaccaat caccggatta ttcgaatgaa tctagacggt
    17461 gaaagctggg gattccaata ttctccttat gcttattaca atgaacattc gatcatttta
    17521 tcagaacaac accgcccgat gaaaatcaca aaggaaacat ttgctagact gcttcggatt
    17581 actgaagtct tgcctcatta ctttgttggt tcgaatgctg acttgccgat tgttggagga
    17641 tcgattcttt ctcatgatca ttatcaggca ggacgtcatg aatttcctat ggcaaaagca
    17701 ccgatagaaa aacttatcaa actagcagat tatccgcaag tcatcgcagg agtcgtgaaa
    17761 tggccgatgt ctgtgatccg tttgcaatca acagagaaaa aattgttgat tgaagcagct
    17821 aactcagtat tggaaaagtg gaaagcatat tcggatgaaa cagtatctat cagagctttt
    17881 tcagcagatg gtacaccaca ccatacgatt acgccaattg cacgaagaag ggacaattac
    17941 tttgagttgg accttgtcct tcgagacaac aatgtttcag aagaacatcc tgatgggatt
    18001 tttcatccac ataaagatgt ccaacatatc aaaaaggaga acattgggtt gatcgaagta
    18061 atggggcttg ctgttttacc acctcgtttg aaaccagaac tagtagaagt agagcatttc
    18121 ttattaggag aagaaaacca gatggctgaa tatcatcgtc cgtgggcaga acaattaaaa
    18181 aaagaacatc ctgatttgac gaaagatacg gtcaccgatg tcgtacaaaa atctgtaggt
    18241 caggtttttg cacgtgtact ggaagacgct ggagtattca agcgagatga aaaaggacag
    18301 gcagcgtttg aacgatttac tgcaatttta taaacgtaaa aaaggaggag agacaaatgg
    18361 caacaattaa agacattgcg cagttggcag gagtttctcc tgctactgtt tcaagagttc
    18421 tcaactatga tcctgatctt tccgttggct tagccacaaa gcaaaaaatt tttgaagcag
    18481 cagaacgctt aaattatacg aaacatcgta aaaatgcagc aaatactaag caaacgcttc
    18541 gcctgatcca gtggtacgac gatacggaag agcttgaaga tctttattat ttatctattc
    18601 gtctaggaat cgaaaaaaag gcagaagaag caaacgttac tttattgaaa gaaacgtggg
    18661 attccattag tgatcttccg acagatggaa cgatcgcact aggaaaattc gacgaagctc
    18721 agttattaga aatgaaagga tcccaaaatg ccttgctttt ggtagattct gacggcactt
    18781 ctcaaggaat cgactctcta gttgtcgact ttaagtcaag tgttcaaaaa gtcattgaac
    18841 attttgtctc ccataaagcg caacatatcg ctatgcttgc tggaacagaa tatacgaaga
    18901 aaaaccatca gcggctaaaa gatcccagaa ttatggcatt tcaacaagtt ttggcagaaa
    18961 aaggctacga cgaaggtcca attatcgaag cagatttttc tgtcgagtca ggatatcaag
    19021 cagtgaagca gtatttaaaa acgaatccgc atgttcctga tgcgcttttt gcagctaatg
    19081 atgcattagc gattggcgca ctgaaagcga ttcaagaaaa aggtttagtg gtaccagaag
    19141 atatttctgt gatgggtttc aatgatgtca gtgtagcgaa atatgtaacg ccctctcttt
    19201 ctactgtaaa agtttatacc gaatggatgg gagagctagc agtggagacg attcttcaat
    19261 taagtaattc caaagcaccg gtttcaagaa agattattgt ggaaacagag ttgattattc
    19321 gagaatcaac gaaataaa
//
LOCUS       DAYYCW010000055        18803 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000055 DAYYCW010000000
VERSION     DAYYCW010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.055"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..1526
                     /locus_tag="ACHAYA_001707"
     CDS             99..1526
                     /locus_tag="ACHAYA_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8605504.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1618..1983)
                     /locus_tag="ACHAYA_001708"
     CDS             complement(1618..1983)
                     /locus_tag="ACHAYA_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8605505.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1980..2345)
                     /locus_tag="ACHAYA_001709"
     CDS             complement(1980..2345)
                     /locus_tag="ACHAYA_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8605506.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2537..2785)
                     /locus_tag="ACHAYA_001710"
     CDS             complement(2537..2785)
                     /locus_tag="ACHAYA_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605507.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2784..2912
                     /locus_tag="ACHAYA_001711"
     CDS             2784..2912
                     /locus_tag="ACHAYA_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM8605508.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2969..4663
                     /locus_tag="ACHAYA_001712"
     CDS             2969..4663
                     /locus_tag="ACHAYA_001712"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM8605509.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4763..4936
                     /locus_tag="ACHAYA_001713"
     CDS             4763..4936
                     /locus_tag="ACHAYA_001713"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605510.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5314..6447
                     /locus_tag="ACHAYA_001714"
     CDS             5314..6447
                     /locus_tag="ACHAYA_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM8605511.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6478..6798
                     /locus_tag="ACHAYA_001715"
     CDS             6478..6798
                     /locus_tag="ACHAYA_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM8605512.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6827..7291)
                     /locus_tag="ACHAYA_001716"
     CDS             complement(6827..7291)
                     /locus_tag="ACHAYA_001716"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8605513.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7423..8676)
                     /locus_tag="ACHAYA_001717"
     CDS             complement(7423..8676)
                     /locus_tag="ACHAYA_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8605514.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8705..9022)
                     /locus_tag="ACHAYA_001718"
     CDS             complement(8705..9022)
                     /locus_tag="ACHAYA_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8605515.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9048..9368)
                     /locus_tag="ACHAYA_001719"
     CDS             complement(9048..9368)
                     /locus_tag="ACHAYA_001719"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8605516.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9832..11247)
                     /locus_tag="ACHAYA_001720"
     CDS             complement(9832..11247)
                     /locus_tag="ACHAYA_001720"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8605517.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11264..12580)
                     /locus_tag="ACHAYA_001721"
     CDS             complement(11264..12580)
                     /locus_tag="ACHAYA_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8605518.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12730..13428)
                     /locus_tag="ACHAYA_001722"
     CDS             complement(12730..13428)
                     /locus_tag="ACHAYA_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM8605519.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13717..14706)
                     /locus_tag="ACHAYA_001723"
     CDS             complement(13717..14706)
                     /locus_tag="ACHAYA_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8605520.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14786..15619)
                     /gene="msrA"
                     /locus_tag="ACHAYA_001724"
     CDS             complement(14786..15619)
                     /gene="msrA"
                     /locus_tag="ACHAYA_001724"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8605521.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15794..17428)
                     /locus_tag="ACHAYA_001725"
     CDS             complement(15794..17428)
                     /locus_tag="ACHAYA_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM8605522.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17415..18800)
                     /locus_tag="ACHAYA_001726"
     CDS             complement(17415..18800)
                     /locus_tag="ACHAYA_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605523.1"
                     /translation="MCTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAI
                     AEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIV
                     RLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEA
                     LSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTN
                     LFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFL
                     LVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKH
                     EVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKH
                     GLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVT
                     KKEINKKNENK"
BASE COUNT         5947 a         3706 c         3142 g         6008 t
ORIGIN      
        1 ttatatgctt tttcagaaat taactgatga tttcaaaaag aaaacatgtc ataatatttc
       61 ccatattgtg caaaaaaata caaggcggag gctctactat gattgaagaa tatattgaga
      121 aaaatatttt aagacagctg tttttatgtg ggcaattcta tgtaaataaa gaagtcaatc
      181 tagaaaagct ctccaatctc ctgcatgttt gcaaaaccac tttactcaat gatatcaata
      241 acataaaaaa agaatttgaa gaacagatcg cttatacaca tcgggaaaag gaccgttaca
      301 ctttatattt ttcagaacat atacctcgat gcaagatcat gcagcaattg tctcaaaatt
      361 cgttgttttt aaaaacttgt ctgctttatt tggaagaaga tgaacctgat tatcttcaat
      421 taactgaatg tgaattcatc tctgtcagca aagcttattc attaaaaaaa caagtattag
      481 cttattttaa tgactgcggg atcgaaatcg accgctactc ccctcgattc actgaaatgg
      541 aaagacgtct gctgcttctt aatgtgtcgt atcgtttagg cggttttaat tcttgggagc
      601 tacctgaatc gttttttgaa cgtgccgatc gtttcattga atccgtaacg gaaaatagtg
      661 gcagatttta tgataaagaa aataaagaaa tcttatcgat tggttttgct atcagtttcc
      721 tacgccagca agtgtgtgct gtgacaatag attcaaaatt tatcgaagaa atcaaaaaac
      781 gtcctattta caattatgtc gaatcagcat gggaaaacac tgatttccaa acctattata
      841 aaaaagaaga attcgccttt attttgacac tattcaatct ttgtaattat ggtttccatt
      901 cttatcagtt gattgcggaa gactttcaac agctacacca agtgtttatt gacaatactc
      961 ccgaaataaa agaactagtt gctacctttg aatctcactt caatcaagaa ttattcggaa
     1021 accaaccgtt cgaacgtgcg ctgattcatc tgatgcgttc ggcttgggat aactatcaat
     1081 tattcatgcc agaaaaattt tatcttttaa atgaggaaca gacaaattta tataaagaag
     1141 tccagacaat tttttccagc tggtccagcc agttacctta tgatctacgg ttgaatccta
     1201 attgcatgcg ggcttttgtg atcgaattga gcggaattct ccgtctgaca aaagaacatc
     1261 tgactatcta tatcgtcaca aattcggatg ttcattatct gatttatcgg gaagccttgg
     1321 aagctgtaac tactttcgat tttcaagttg caccaacaat ttattcaagt atatctgata
     1381 taaagaagta tgcgcagcag tcctcaaacc gggtattatg tgaacgtaca ttatatacac
     1441 cagatgcggt ccaatatgaa aatattattc ctatttcgat caatacgatc gatcgagcta
     1501 tcatttcagc tgtgcaaaac aaataagggg ctgtgacaaa aatcatgtca cagcctctta
     1561 tttgttgaag atgcccattt ctgtaacaat ctcttgatac acgtcaggct cacaacctca
     1621 cttattttgg acatctacgg gtccgatctc tttatttaaa aaataagaaa tgatggttcg
     1681 gataactacc aacgccgcta gttggaaaat atcctgcaag gtaggtttga taatcgattc
     1741 gatgatccca gctgaaacaa gaatttccaa acttagaagt atatagcctc ccaacatctt
     1801 tttaatagca ttgttctcgc tgactcggtc gctttgcact tttgtatgga caaataaata
     1861 tttcaaaaaa tccttgattg atagacaaac accccaaacc aataaacaga tagcaaagat
     1921 atcaagtccc agtacaagtt tttcgaaata tggaagaaga aactgcatca actctgtcgt
     1981 catcctacct ctcctcttct ttgtttttct ttgtcttcta tttcctgatt caagaaataa
     2041 gaaatgactg ttcgaatgat aataataagt gcaagcttca aaatatctgt ccaagtcggc
     2101 ttgataactg actcgatgat atcagcaacg actagtactt ccaagcttaa caaaatataa
     2161 cttgctagca gttttcttgt atctgcatta cgagtattac gttctgcata gtttttcttg
     2221 acatgagacc aatcaaaaag tttgaataga acaagaacga tcccgacaat taagataccg
     2281 atagaacaaa tatttaaaat agcaatgatc cattctagaa attcaattaa atttgagcga
     2341 aacatatacg ctacctactt tcttttaatt tgttcctatt cttttattgt aaaagaaagt
     2401 taccttgttc gtctatttaa aagttgttct aatgataacg gttcgcttaa atttttcttt
     2461 ttcactctcc catttttctc cctccatcca taaatcgtgg aagtatccaa aacaacgtag
     2521 caagttaaaa taaggcttag tccgtcatca gcttttcaaa gaagcactgt ctcggtgctt
     2581 tttttgtacc caaatttcta aaaatagaac catacacctc ctcaaatctt ttcttcttac
     2641 ctaccctatt tttatttaca ggaaaatcag acacttcttc ttttttgtca aaatttcctt
     2701 tttcttctcg tcctatcatt tggcattcta ataaaaacga actatccttg gatatagaaa
     2761 gagaaaacaa ggaggcgttt ttcatgagta aaaaattaat caaagtcggc atcggtttag
     2821 gtttgcttgc ccttggcgct gcatatctag gaaaaaagac aggactattc gaagatgaca
     2881 gtcatttata tgatgaattc gaatcaatct agcatcctat ttagttgaat cactacggac
     2941 atacttggaa gacattggag gaatcacaat gagaaaaagt aaagcaataa tgattggtgc
     3001 cggtattgcc aatatggcag cggctgttta tttgattcaa gaaggcaaat ggaaagggga
     3061 tcagatcacc ttttattcat tggatgatca cggttcaaat gatggtgcac cgaccgacac
     3121 tgtagtggat gaatattgga ataaaaacca tccattagaa aaccaaaaag gctatattgc
     3181 tcgaggcggt cgcatgctta attaccgtac ctatgtagat ttgatggatc tgttaagtcg
     3241 aataccttct gttactgagc cagggatgac tgctgaagaa gatacgagag attttgatgc
     3301 aaaacaccga acctttgaca aagcacgttt gatggaaggt ggcaaaggaa tcattgatgg
     3361 cggaaaactg ggattcaata ataaagaccg tattcttctg actaaactga ttgctatgcc
     3421 tgattcagaa gaagaaaaac tagacaatat cactatcgaa gaatatttca aagatgatcc
     3481 acactttttt gaaacaaact tctggtttat gtgggaaaca acatttgctt tcagaacacg
     3541 tagttctgct caagaattgc gtcgctatat gcatcaaatg atttatgaat tcactcaaat
     3601 cgaacatcta gtgggcgtca atcgaacacg ttacaatcaa tacgaaagta ttatgcttcc
     3661 tttgatcgag tacttgaaag aacaaggatg caaaatcatt ttgaaccgcc gtgtcactga
     3721 ctgggaattc aaagaaacac cgatgcaaga tgaaatcact gtgactggtt tgaaaattac
     3781 caatacactg actcaagaag aagaatacgt tgtggtcgat gaagatacag cagttatttt
     3841 cactaatggg tccattacag attcagctac tttaggagat tacgaaacac cagctgtaga
     3901 aaatatggat tatggtgcag cttctagtct ctggaaaaaa gccagtgaac gtttctacaa
     3961 cttgggaaat cctgacaaat tctttgctga tcgagatgca agcgaatggg tcagctttac
     4021 cctgacgacg aaaaaccatt tgttgttgaa tgaaatcacc cgtatcacga ctcaagaacc
     4081 aggaaatgcc ttgaattcat ttatttcaac cacaccgatc acgccgttag gccaaaaaga
     4141 tgtcaatatg tctattgtcg tgcatcatca accgcatttt acgtctcaga aaccaaatga
     4201 aacagttatt tggggttatt tcctttatcc aagacgacgt ggcgaatttg tggataaaca
     4261 gtatattaaa atgaatggaa aagaaatgct agaagaactg attggacaac tttcaaaagt
     4321 agatcctgga ccagtgaata ttcgtgaaaa agaaacagaa attttcgaca gcgtaataaa
     4381 taatatccct gtctatatgc cttatgcttc cgcgttgttc aataatcgtg ccaaaagcga
     4441 tcgaccaaaa gtcattccta aacattcgac caatctggcc tttacaggag aatttgtcga
     4501 gcagccttat cagatgatct ttactgaaca aagtgctgta cgttctggag aaatcgctgc
     4561 tttccatttt gcgggtattc ctatgtcccg tcttgtcaaa acaccgcgtt acgacaaaga
     4621 tattcctact ctagctcgtg ccgcaaaaaa aatgtttgaa taatcaagag gttgtgaaaa
     4681 agctgccctg catcaagtaa taagaacaac caggctaaaa cagcttctta tgtttttgat
     4741 ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttaccaa gaggttatga
     4801 aaaagctgcc ctgcatcaag taataagagc agccaagcta aaacagcttc ttatgttttt
     4861 gacttggtta gacttattac cgcagatgca agcttttgaa caccgtttac caagaggtta
     4921 tgaaaaaggc gtttagtttc gagtatgaag aaaaattttg tgaaaatggt tcctcacgtt
     4981 tttgcataaa tttgacttaa taccgagaaa ctgatccctt ctgtcataac ctctttttat
     5041 ttatgtctgt ttctttttga aaacaactat tttttcaacg tttactctct tgccaatcca
     5101 acatcatcta acggatacaa ctatttcaat gattaccatt acactaaaaa atcggaaaca
     5161 cacctctccg aacacaaata cataagacta tagaatgttt catttaagaa aaaaagcgtt
     5221 tcattatcaa aaaggtaaga tctcctttaa aatgaaatta tctgtagtac taataatgca
     5281 gacaatttta tgaatctgaa aggagcatct ccaatgaaag cagtcacttg gcaaggaaaa
     5341 cagaaaatgg aaatcagaaa cgtagatgat ccaaaaatat tagaaccaac agatgcaatc
     5401 atccgtatca cagcaacagc catttgcggc tccgatctgc atctttacca tcatggtgaa
     5461 gcagttttgg aaaaagggta tgttgtagga cacgaaccaa tgggaatcgt tgaagaagtg
     5521 ggatctcaag ttcaaacatt gaaaaaaggg caacgtgtgg ttatcccttt taatattagt
     5581 tgtggtcatt gtcatttctg cctgaatcac atggaaagcc aatgtgatga atcaaatcca
     5641 gatcagttat atggcgggct tttcggattt ggcaagttga acggtaatca ttggggtggt
     5701 caagcggaat atctaagagt tccttatgca gactctactt catttgttgt accagataat
     5761 gatcttcctg atgaaaaagt tttatttcta tcagatattt tgcccacagc ttattggagc
     5821 gttgaaaacg caggagtaaa aaaaggcgat acagttgtga ttctaggttc tggtccagtc
     5881 ggtctattcg ctcaaaaatt cgctgttatg gctggagcag agcgcgtaat cgcagtagat
     5941 ccagtacagc accgtctaga taaagcggct aattacaatg gggttgaaac ttatttctta
     6001 gaagacacgg cacaagctgg aacagaattg tatgaactgg ctaaaggtgg cgcagatgtc
     6061 attatcgatt gtgtaggaat ggacggatta gagcctgtca aagaaaaagc gaaaaattta
     6121 gtaagcttac aaagcggcac aatctctcca ttacagatgg catcgcaagc tgtcaaaaaa
     6181 tttggaacag tccaagtaac tggtgtttat atgactccag cttcttctta cccgctgcaa
     6241 gaatttttta tgcgggatat cgctgtcaag catggacagg caccaattat ccatctaatg
     6301 ccaaaaattt acgcaatgat tgcagaagga ctattcgatc ctacacaaat cattactcat
     6361 tcgctgccac tatcagaagc agcacaagca tatgagatct ttgacaaaaa agaagataaa
     6421 aatataaaag ttgttttgaa accttgaatt cagtaaaaaa tacaggtgat tttctttatg
     6481 cccaaaatca aacgaaatac ccatgagatc gaaataggaa aagaagaaga tatcgaaata
     6541 cgaggacgtc gcttccgctt ctatttccaa aatcgttaca cattgatctc actggctgtc
     6601 gatttattaa ccgggatttt ctatattttt ggaagccttg cttccttaac taaaatcccc
     6661 gatcgatatg gcacctattt ttatttggct ggtgctattt tcttgacgat tcgtcctatt
     6721 ctcagaatcg taaggaacat ttttatttac gacgaaaaaa aagcaatgaa gagaacaagc
     6781 aaaaaaaaag aggcatgaga atttttcaaa tctcatgcct cttttttcac cctatcgtat
     6841 cactttgata cagacgagcg gtcgtcccac tttcttcctt gaccattgtc aaaaatgtcc
     6901 actcgtatcg ttcataaaac gtggtatgat ctgtaattaa gtaaagtttt tcgtacccaa
     6961 gttgatgcat ttcttttctg atttccgcta atatccgctt ggctatcccc tgcttacgct
     7021 tttccggttc aacaaacagg gcacaaagat ttggtgtcag atcagggcgg ttatggaagt
     7081 cgttttcaat gacgcccgct cctccgacga tggtttcttt ttcatctaag accacatacc
     7141 attgaggaat actttcctta ttagcgattc cagtacgaat actttcctca tacacaacag
     7201 caggaactcc ccattttgat gaaaaccaat tcgcgctttg ttttactaat tctgggtgtt
     7261 ctcttaattt gatccagtta tatgtaacca atgtttaaac ctccgactaa aataaaacgt
     7321 ttacaaaaat tatacaagtc tttatgaaaa ttagcaatga caacaatagt gatgtaaaaa
     7381 aaaaaacgga aaacaatcac ttgttctccg ttttttgatt aatcaagcat ctaaagcttc
     7441 ttttcttctt tcttggatcg tagctacctt gacgaacggt agatagatca aaatagaaag
     7501 aaccaaattg acagcggcta gtacaccacc tgcaatactt tttgttgcca atacaccgcc
     7561 aataattggt ggagttaccc atggaggcat aacggtagct gctggcacta aacctgtaga
     7621 agtcgcaaag taagcaacac taactaagac cattggtgtc aaaataaatg gaatgaacat
     7681 gatcgggttc aacacgatag gtaacccaaa catcgttggt tcattaatat tgaagacacc
     7741 tggcgcaatc gacaagtttg ttacaaccat atatggtttg tttttacgtc ctactaaata
     7801 aatagctaac agtaaaccta aagttgcacc tgttcctcct aagttgacaa agctgtcaaa
     7861 gaaaggtttg ttcacgataa acggtgcgct atgtccagct gcgattgcat taacgtttgc
     7921 ttcaatggct ggcgcattga ttgtttgcat caaaggatcg accatattcg ctccgtgtaa
     7981 accaaagaac cataagaatg gtgtgataaa tgcaagcagt aaggcagatg gataagagtt
     8041 tgcaagtccc ataaatggtt cttggaccac tgtatagaaa gaacctacga tatcatcaac
     8101 accgaatgca gcaaaaattg cagcaactaa agcaaacgca gagatagtga tcattgctgg
     8161 tagtaatgaa gcaaatgctt ttgcaactgc cggtggcaca ccatcaggca ttttgatgat
     8221 caatttttca tttccgctta aacgtacaaa gatttctgtt gaaataatcc caacaataat
     8281 agcaataaac aaaccgtttg agctcatacc taatgctccg cctaaagcga agaaagagcc
     8341 taaagaaatc acacctgcgg aaacaccgtc ttttcggtag ccgttagcta aatgataagc
     8401 aagcaagaaa gcaatcaaga cagaaagaat ggcaaatgta ccgttccaag cagcagcccc
     8461 gaatcctttc catgattcac caccaaaaat tgagttcatc aattcttgga acccgggaat
     8521 cggtaagtta ttgatcatta ctgctaatgc tcctaagatc attaacggca tactcaccat
     8581 aaatgagtcc cgaatcgcaa ctaaatgccg ttgtgcacca atttttgatg ctacaggtat
     8641 aaaatgtttc tccataaatg caacaaattt atccataaat cttccctctt ttctttactg
     8701 tagattacgc gttcaatcga cgatacagat caatgatctc ttttgctaaa tctgtaaaag
     8761 ctatagatgt catcagatga tcttgactgt ggaccgtcaa aagcgtgact tcaacgtgat
     8821 ttccttgtgc ttcttgtgtc agcatctctg tttgagagtg atgtgcttgg actaaagatt
     8881 cctctgcatc tttgattttt tcatctgcta aagtgaaatc accttttttc gcagcagcaa
     8941 tcgcttccat tgcatcgctt tttgcatttc cgccaaacat gatcagaccc atgatggctt
     9001 ctagattttt ttgatcttcc atttattgta tcctccgata atttttatta tttgtttatt
     9061 agtttttctg cttggtcgag tactttttca ccattcatca tgccatagtc agccatattg
     9121 attacatcta aaggaatgcc tttaggtgct agcttttcag caaattgatt ttgcataaag
     9181 cgtacttgcg gtcctaaaag taaaacgtct acatttttag attctagttg attatccgca
     9241 tctgaagcag atacagcaaa gatgtctgct tccattcctt ttgcttctgc tgccttttgc
     9301 atttttgtga ctaggagact tgtactcatt ccagctgaac atacaagcat gattgttttc
     9361 ttttccattt taaacacgtt cctttctata cttacacttg tatataagca agtagcgtgc
     9421 caactttaaa agcgctttaa aaagttgata tcatcacatt taaggtctat accaagttgc
     9481 atacacacta aaaaagaaag cgcacactaa acacacaaat taatttttat tacctttttt
     9541 cttttaacgt gcacaaaaaa agttgtgaca caaaacttgt cacaactttt ttcaaataaa
     9601 cggtattcaa aagcttgcat ctgcggtaat aagtccaacc aagttaaaaa cataagatgt
     9661 tgtttttact ttgctattct tattacttga tgcaggacag ctttttcaca acctctcaat
     9721 aaacggtgtt caatcagctg accctgggta ttagctcaaa acgctggatt cacaacctct
     9781 tttatttcaa ataaccagta aatacaatga acatctacaa aaaacttcta tttatttata
     9841 gatttcgtca ggcaaatcca attctctctt atcaatcaaa gtctgatacc acgaagcaga
     9901 ttttttcaat gttctgcttc gttcatcgtt caattcgatt cgaactaatc catatcgatt
     9961 cttaaatgca ttcattggtg acacacagtc agtgaatgcc cataacatat agccttcgca
    10021 atttgcgcct tcttctgttg ctttgatcaa gtaatgcaaa tgctgtccga taaattcgat
    10081 acggtaatca tcttgcacga ttcctttctc atccatatac tgttcttcat attctcttcc
    10141 cataccgttt tctgttatca gccaagggat attatgataa ttttctttta gatacatggc
    10201 tatatcatac atgattttcg gatagatttc ccatcccctg gaattattca ttttcttccc
    10261 aggtaaatca aagacttcat aatatcgttc aggatggaaa ggtgtctctt cattccattg
    10321 ataacgaggg gcgcgaacgc gtttaggata atattgattg attccaagga aatctactgt
    10381 atgatgctga atcgtttcta aatcctctgt tgcaggatcg aaataaatcg attgtttttg
    10441 acacaactcc aggaactctt ctgtgtagta tccttttacc atcggatcaa aaaatactcg
    10501 attataaaac aaatcataca tataagcagc tttttggtct tctggagaat tggatcgagc
    10561 atagaccatt tcaggattta aaatacagcc aatccttcca ccataatttc cgctgcggta
    10621 aatctctact gctttagccg ttgctaaaac tttatgataa ttccacatca tccacttttt
    10681 tgtgttttgt tcatgcggcc agcgaacagc atccaaatag caacgcgttt ggggaacgat
    10741 tggttcgttg aatgtaaacc agttttttac cctatcaccg taacgatcaa aggcaatttg
    10801 agcatactta gcataaaaat caactacttt tcttgaagac cagccatcat atttttccaa
    10861 aagaaaagct gggacttcat aatgttccaa acaaatcatc ggttctacgt ctgcttcgat
    10921 caaagagtcg atcatttcac ttatatgctg agcatattct tcatctacta tacagttctc
    10981 ataatcagta aaaaaacgag cccaattgat agaagtccga taatgtgtca gatggatgga
    11041 tttcatcaga tccacatcct gctggtattg ttccataaaa ttagttgcta cggctgggcc
    11101 atacccatta tgccaaacaa aatgttcatt tttataccag ctatctataa acgaatcctg
    11161 tccttctttt tttcctttcc aaccttccgt ttgccaagca gaagatgcag caccaagaat
    11221 aaaatttttt ggaatagtaa ttttcatgat ttcctcctgt tttttagatc ccatcatttt
    11281 ccacacgatt tgctactttt acaaatggca gatagatcaa aatagaaaca ataatacaaa
    11341 caagttgcgt aataactgca ccgatatttc ctgctgttgc cagataagca ttgacgatcg
    11401 gcggcattgg ccaagggacc attacaactg ctttcgccgc aaatcctaaa gcggttgcaa
    11461 aataaccaat cgttccagtg ataagaggcg ttaagataaa cggaatagct aaaattggat
    11521 tcagcatgat cggcatacca aaaataactg gttcattgat gttgaagatc ccaggagcaa
    11581 gggataactt ggtgatttgt ttcatatctt cccgttttcc aactaacaat actgcaatca
    11641 gtaaaccgat cgtgactcca gacccgccca tactcatata catatcccaa aatggcatag
    11701 taataatgtt cggtacttct tttccagctt caaaagcttc cgtatttaca gcaattgccg
    11761 ctaaaagcaa cggttcccgt atcggcttca ccatttgatt tccatggata ccaatcaccc
    11821 aaaagacttg cgcaataaac atcaatagca aaatacccgg taacccttgt acgacacctt
    11881 ctaatgggcg ttgtacgaca ttgtaaatga tatcataaag gtacattcct gtaagttctt
    11941 tgatgataaa tcccgcagcc gcgatacccg atacagtcag aatcgtcggg aataaagcag
    12001 aaaaactagc agaaacattc gttggcacgg tatcaggcat tctcagctgt agtttcttct
    12061 gtttcctcaa ccagcaataa agttcaatag aaaggatgga gaccaacatt cccaaaaata
    12121 accctttcgt atctgtatat tgtttagcca atacgttttc tactataaca gtctgatttt
    12181 cattcactaa ttcataaaca gttggattaa cagataaata agacatcact gcgatgatac
    12241 cagggaatac accatccact ttattcagct tggctaattc gatccctatc agaaaaacag
    12301 catagatggt caagaaactt agtgtgacat agctgatggc agatgcgata ggctttaatt
    12361 cttctaaaaa acgcaagaaa cttatctgtg ctaagccatt ttccgagtca aagaccattg
    12421 ctgaaaacaa tgtgccaaaa gcacccgtaa taattaccgg aatcaatgca gcaaatgcgt
    12481 tttttatcac catgatatac cttaaattat tgattctcgt tgccacgttc cctaaaacat
    12541 tggctaattt atccataaaa ctcattccag atcccctcat tcctgcttct tctccttgac
    12601 gtcatctctt tttgtctatt agtcagcgct tacatttcga tgaacagaaa ctatttactc
    12661 ctctttttta ccaaatataa aacaaaaatt catctgatgt atttatcata aaatacatca
    12721 gatgaactgt caattagttt ctttttctcc tattattttc tgtaattctc tccgatttat
    12781 tgccatatgg acagtcattg catcaaacgc agcaagtgca ttccgccttt cgatggcctg
    12841 aacgatctct gtatgaatat cgatcgtctc gattttcaga tgctggatat cataattttt
    12901 attaaacaaa gcaattgatt cattaatgat tgggataata tgcagcaagg ctacgtttcc
    12961 gctagcttct gcaatcatcg tatgaaaatc gatgtctaat tgaacatgtt cttgtgtgcc
    13021 ttctcgaaaa gaagtttcaa tcttttgcgt taattttttc atttggacga tttgattctt
    13081 cgttgcatat tccgctgcca aacctgccat cttaggctcc aataaatatc tcagttcaaa
    13141 taagtctcgt atcatccgat cctgatcttc aatcaaagaa aaaccgagtg ggtcattggc
    13201 aatccctcgc ttatcactga caaatgtccc cgctccttgc cgaacttcaa ggatattcct
    13261 ggaagctaga gcccgcacag cttctcgcac cgtgcttcgc cccacctcta aataatcgga
    13321 caattcatat tcattcggca gtttatctcc tacttgcaac tgcatttcct ttaaatatct
    13381 aaggatcttg tcagtagtta cttctactag ggttttattt tcttccaaac cacatacctc
    13441 cttttcatct gatgtatttt ttcgtaatta ttataacacg tttgaatcat ctgatgattt
    13501 tttgtataca aaattgatta atccgttcat agacatacaa acgaagaaga tatttaccat
    13561 aaaaaagact aggataaaat tcactcctag ccttcgatca tttttatttt gaacgacaaa
    13621 aaatatgatg ttcaataaca gacgatcttc attcgattct tgatgtcatc ttttgttttt
    13681 tttaatatat ggataaaaag tttgattaca tcgtttctat tctagaattt ggtcgattct
    13741 caattcttct tctggtgaga aatctaaatt atccatcatt cgaacacttt cttgtaactg
    13801 acttaaccga cttgctccga ccagtacact tgtaacagat ttttgtcgca aattccatgc
    13861 aagggccatt tgcgctaatg attgtcctcg tgatacggcg attgtttgca gtgctttgat
    13921 tttttccaga gttgatccaa cttgttcttc tgaaaggaat ggtatttcct tacgatgtgc
    13981 tctagaatct tcaggaatac catgtagata acgatttgtc agcaaccctt gagcaagcgg
    14041 actgaacgtg attgccccta gcccttcttc ttctaatact ggagatagat cgtcttctag
    14101 tagtgcacga gaaaacatat tgtagcgcat ctgatgaata atgaacggtg cttcctttct
    14161 ttttaaaatt tcagtcattt tttttgtatc ttctccatta tagttggata taccaatata
    14221 taatgctttt ccttgtctta caagttgcat caaagcttct gctgtttctt ccaaaggtgt
    14281 atcagggtct gggcgatgat gatagaagat gtcaacataa tctagcccca ttctttggag
    14341 actttggtca caacttgaaa tgatggattt acgagagccc cattccccat atggcccttc
    14401 ccacatatga taacctgctt tgctagaaat aatcagttca tcacggtagg cttgaaaatc
    14461 ttctctaaaa atacgcccaa agtttttctc tgcagctcca gcagggcctc cataattatt
    14521 cgcaaggtcg aaatgggtaa ttcccatgtc gaatgcgcct cttaaaatct ctctttgatt
    14581 atggatagga tcataatcac caaaattttg ccataatccg agagataaaa tcggtaattt
    14641 caaacctgaa tgaccggatt tacgatattc cattttttca taacgtgcac tatctgcaat
    14701 atacaatttt tctcctcctc atcaaatctt tttaaaacgc tttctttttt ctatttttag
    14761 tttactacat tttaaagaaa aggagttaat caaaatgatt ttttcgtata tttttttgcc
    14821 atttgttttt tactcgttga tagatcagaa attgattacg tgcttgctgg atttttttat
    14881 aacgtttcgg ttgttttttg taaaattgct gatgatagtt ttctgctggc caaaacgtac
    14941 cggctggttc gatttttgtg acgattggtt ggtggaaagt tcctgaactg tctagtttct
    15001 gttttgattg ttccgctaat tctttttgtc gttcgacttg ataaaaaatc actggacgat
    15061 attgcttccc tctatcttga aattgtccaa atgtatctgt gggatcagta atttgccaat
    15121 agattgtcag caactcgcta taacttattt ctcttgtgtc ataaatgatc tccacagctt
    15181 ccacatgacc tgtgtaacca ccacttactt gatcataatt tggtttttca acatgtccgc
    15241 ccgtgtatcc tgacaaaaca gataggatcc cttttttcga ttcaaacggc tcgaccatac
    15301 accagaaaca tccccctgca aatactgcac gttcttgata gtcagcatct tgcatggcat
    15361 gcttggaaaa gtcatatttt tgctccttat caaatccttc cgtaatcaat aaataaaagt
    15421 ccattacatc aggcgtcaga ttcattcgga tagctaatgg acgtagtgtt gcttctagtt
    15481 ttgataactg ttctttaacg gatatatttt ctcttatgtc attctttgtc tgaatcaata
    15541 cttttcgttc ccaatctcgt gtattcggat tcaaaatcag attataaagt gtttgaatga
    15601 atatttgttc ctcatccata gattctcctc ctttccgaag aagacaagct agtgacttta
    15661 gctgtaattt atcataaagt gaggaaataa gcgattactt catcccccta cagatttttc
    15721 gtataaaaaa gagagaatcc ttatcgacag attctccttt ttaatcaatt tattttataa
    15781 atgaaaagtt aatttacctt ttatggaaaa ctattttaaa aaataaacat aaatatgcag
    15841 ttaatgtaac aagataagaa ataatagtcg ttactgtata acttgtgctt actttaatat
    15901 cagcaactcc tttgactccg cttatttgta ccaatccatt agcattcacc tgctccttta
    15961 caggttgatg atttatttct acattcgttc ctaaataagc atatacagga agtgtgatta
    16021 cttggctctt ctcattagtt actctatata tgatagaact accagttgtt ttataaggaa
    16081 ggtttaccca ttgactgcca atctttgctt cgtgttttat tatatgctcc ttattctcca
    16141 atgaagcgat aggagaataa tcatataaaa aataatccat tcctttagtg gtatacgcct
    16201 ctactgtttc tttgtttaca acaatatgtc ccggctgaga aattattttt tgattgaaat
    16261 tttttttact agaaacagtt aaagtgatta ataacaaaac agtcataaat aaataaaata
    16321 gttctcttgt cttattgtat cttctatttt tgaaaaatat catcgttaat atcagagaag
    16381 agaatattat ttgcaagctt aagatccgcc aaggaaattg taacaatttg gctggcgtat
    16441 tttgcagaag ataccatggc accaaactag ttgtagctag ccaagtcaaa cacgtaccat
    16501 aataaatctt tttttctatg tttgttaatt ttttaaataa aatcagggga atacataaag
    16561 ataacagacc aactagtccc attgagtaag tagttaagtc gttgttcata gcttcagtca
    16621 gactatcgaa cacattagat gcatgagcat ataaatttgt cacgctaggt ttgttgattt
    16681 tttggtagag catttgttca aacatcggat accaaaaagc actcgtcaga accaatgtca
    16741 ttcctgccgc actaaataat tttttaaata tagcaataaa atctgcattc ttgaaacctt
    16801 tagaaaccaa acgaataaaa ataaaaatga taattaatgc tgtactcatt aatgttgata
    16861 gtacatggct gtacactagc aaactcatac tcaatgctag ctttacagac ggatactttt
    16921 cttcttttcc agagatgatt tgataagcat aaaataatac agggataaca agtgttattg
    16981 cgatagcttc ggcaattgcg gaacgatagt agatgtctac tgtccgataa gcagagaaaa
    17041 tatagagaca actaaataaa aatgccgctt ttttttgttt taaaaaccgc tcgccataat
    17101 gataagctac gagtgttgta cacaagttca ataaccaaac gtaaataata tagcttacaa
    17161 tcaaattttt tgtcatccaa tataacatta cagctggaaa aaaagtcaga aagggataaa
    17221 aataatttac accatatcca gtataattga atgtctcata attaatcgga ctggtaaaaa
    17281 tagtcgataa ccccttgatt cgagataagt gaaaagaaag atctccagtg gtataaatac
    17341 tctgtgtatt tttgaaaaag atgaaagaat atatactact aactataatt aatccactca
    17401 atattactac ttttttattt attttcattc ttcttgttta tctccttttt agtcacatat
    17461 cgagaatctt tcaacttatt aggtgcaaat cgtttaaaac ttgaatacac cactaaacaa
    17521 taagtcagaa tactgatcca taaagccatg agagcagttg gagtatattt tgttgtaatt
    17581 tctatcttat tttctccagc atttacatcc acattcacta agccatgttt tttataagag
    17641 tcatttacga ctttaccgtt tacttttacc tgtgtaccta aataagcata tactggtact
    17701 gtcacttttt gctttttgtt gcttgccact gtgtaagtaa tgttattatc tgcagcttta
    17761 tacttccctt tttcccacga ttctcctact tttacttcat gtttttctaa gtgaggcctt
    17821 tcttttatcg cttctgttgg ggcgtaatcg tataatcctc ccatatctga tgttgtataa
    17881 cgtgggagtg tttcttcatt gataacaata tgaccgtgtt ttgattggat aacttttgcg
    17941 taattttctt ttgctgctat agacgtgacg agtagaaaca ccacagtagc gccaatgatt
    18001 aaccatgaat attttttatt catagtcttt ttcataaaaa caagcgttat gatcattgaa
    18061 gaaaaaaaga tttggatacc taagatacgc caaggaaact ggagtaagct tatcggtgtt
    18121 ttttgaaaca aaaaccatgg aaataaatta gttacgcata cccatgtaga aactacgcca
    18181 acatagatga ttttttcttt tttctccatt tctttcagta tgaataaagg aaaaatcaac
    18241 gcaaccaatc ctaataaccc catagtgtat gttgtaatat cgttatttaa cgcttgaatg
    18301 atactatctg aaatagaaag tgcttctagc tgcaaatctg ttttgaaggg actgtgtatt
    18361 tcttgataat tcatctgctg caacatagga taccaaaaat agctagttaa tgcacaagtc
    18421 aaaaaaacag cctttactaa attcacgacc acaaatttta tatcgttttt tatttgcttg
    18481 cttaaaaaca aacgaactat aatcatacaa gcaatcaatg cactacacaa tagtgtagtt
    18541 aagacatgag tatacacaag tagacttata ctgacagata aagcgatgac ttttttttct
    18601 ctattgaata tcaatccata agcataatat aaaacagctg ggataatcgt aagagcgatt
    18661 gcttctgcaa ttgcggatcg ataatagata tcaactgttc gataagttgc aaaagtatat
    18721 aaacaactaa ataaaaaagc agcatctatc tttttaaaaa acttttctcc ataataaaaa
    18781 ctaatcagaa tcgtacaaat att
//
LOCUS       DAYYCW010000056        18559 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000056 DAYYCW010000000
VERSION     DAYYCW010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.056"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..801
                     /locus_tag="ACHAYA_001727"
     CDS             154..801
                     /locus_tag="ACHAYA_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8605524.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    930..1146
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1194..3839
                     /locus_tag="ACHAYA_001728"
     CDS             1194..3839
                     /locus_tag="ACHAYA_001728"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM8605525.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4149..5462
                     /locus_tag="ACHAYA_001729"
     CDS             4149..5462
                     /locus_tag="ACHAYA_001729"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM8605526.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5452..6105
                     /locus_tag="ACHAYA_001730"
     CDS             5452..6105
                     /locus_tag="ACHAYA_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8605527.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6160..6852
                     /gene="radC"
                     /locus_tag="ACHAYA_001731"
     CDS             6160..6852
                     /gene="radC"
                     /locus_tag="ACHAYA_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFM8605528.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            7002..7202
                     /locus_tag="ACHAYA_001732"
     CDS             7002..7202
                     /locus_tag="ACHAYA_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8605529.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7456..9135)
                     /locus_tag="ACHAYA_001733"
     CDS             complement(7456..9135)
                     /locus_tag="ACHAYA_001733"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8605530.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9137..9349)
                     /locus_tag="ACHAYA_001734"
     CDS             complement(9137..9349)
                     /locus_tag="ACHAYA_001734"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM8605531.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9742..11472
                     /gene="efrA"
                     /locus_tag="ACHAYA_001735"
     CDS             9742..11472
                     /gene="efrA"
                     /locus_tag="ACHAYA_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM8605532.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11469..13229
                     /gene="efrB"
                     /locus_tag="ACHAYA_001736"
     CDS             11469..13229
                     /gene="efrB"
                     /locus_tag="ACHAYA_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM8605533.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13324..13521
                     /locus_tag="ACHAYA_001737"
     CDS             13324..13521
                     /locus_tag="ACHAYA_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605534.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13674..14159
                     /locus_tag="ACHAYA_001738"
     CDS             13674..14159
                     /locus_tag="ACHAYA_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM8605535.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14263..14856)
                     /gene="clpP"
                     /locus_tag="ACHAYA_001739"
     CDS             complement(14263..14856)
                     /gene="clpP"
                     /locus_tag="ACHAYA_001739"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM8605536.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            15036..15563
                     /locus_tag="ACHAYA_001740"
     CDS             15036..15563
                     /locus_tag="ACHAYA_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605537.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15654..16391
                     /locus_tag="ACHAYA_001741"
     CDS             15654..16391
                     /locus_tag="ACHAYA_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8605538.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16395..17312
                     /gene="miaA"
                     /locus_tag="ACHAYA_001742"
     CDS             16395..17312
                     /gene="miaA"
                     /locus_tag="ACHAYA_001742"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM8605539.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17314..18543
                     /gene="hflX"
                     /locus_tag="ACHAYA_001743"
     CDS             17314..18543
                     /gene="hflX"
                     /locus_tag="ACHAYA_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM8605540.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5995 a         3210 c         4033 g         5321 t
ORIGIN      
        1 attttacgtt tggttgagct tattaccgca gatgcaagct tttgaacacc gttttatttg
       61 tctcacaccc ttttccttcc tacttgtaac cttgctcccg tgttagaaac atgttacact
      121 aaatatgtga aattattaac aaggagcgtc gttatgcgag aaaaaacaga tacactgcca
      181 aaagcaactg cgaaaagatt acctttatat ttacgctact tgaagttgct ggatgactca
      241 ggtataagta gaatcaaatc aaatgagttc agcgaaatca cccaaattcc ttctgcaacg
      301 atccgcagag acttctcgca attaggcgag ctaggcagaa gcggatatgg ctatgatgtc
      361 ccttttctta tcgatgtatt caataatatc cttaatacta aagaagaaaa acgaatcgca
      421 ttagtaggat atggaaatct tggcaaagca ttgaaacaca acaattttag aagaaatgaa
      481 aatctaaata tcgtgtgcgt ctttgataat gatccagcac tgatcaatcg agtaattgat
      541 ggagaaatga tctatccgat tgatcgtttc gcggagatag ccaaagcaaa aaatgtgaca
      601 gtagcaatct ctacggtacc aagtaaatat tcacaatctg ctattgatga aattgtcaaa
      661 ggaaatgtaa ccgctatact taattttgcg cctgacagag taactgtccc agcttatgtc
      721 aatgttcaat acattgattt gacaaccgag ttgcagacgc ttatttactt tgatgagaat
      781 tactcagaag ttttttctta ggaaaatttg agtaggctta ttgatcttgc gagaactcgt
      841 cgtaattctt tcaaaaattt tttagtacta aatgaagtgg ttttttcacg accacttgtt
      901 gactaaaaaa aaaaaccatt gtagaatgaa gcataacgct gaacaagagg agtaactggc
      961 acattcattc ttagagagag aactatctgc tgaaaggttc tcaatgacaa ccagtgaagg
     1021 tagcttggga gtttctgtct tgaatggagt agagatagac gagtcgtgat cgttaactca
     1081 cttgagaggc aataatgatt gattattgca atttaggtgg taccgcgtaa atcatacgtc
     1141 ctagaatacg aaagtatttt aggacgtttt ttagtttaaa ggaggaaaac agcatgacgg
     1201 aagaaaataa cctatcaaca aaatataatc cgcaagaagt tgaagctgga cgttatcagc
     1261 attggttaga acaagatttg tttaaaccga gcggagataa aaaagcaaaa ccttattcaa
     1321 tcgtaatccc gcccccaaat gttactggaa aacttcattt agggcatgct tgggatacga
     1381 ccttgcaaga tatgctgatt cgtcaaaaga gaatgcaagg atatgatact ttatggctgc
     1441 ctggtatgga ccatgcagga atcgcgacac aagcaaaagt agaagcgaag cttagagagc
     1501 aagggatttc tcgttatgat ctaggtcgtg aaaagttcat cgatcaagtg tgggaatgga
     1561 aagaagagta tgcgagccac atccgcgaac agtgggctaa aatgggctta tctttagatt
     1621 acagtaggga acgttttaca ttagatgatg gattatctga agcagtacgt aaagtttttg
     1681 ttaccttata cgaaaaaggt ttgatttatc gtggtgaata tatcatcaac tgggatcctc
     1741 aagcacgaac agctctatct gatatcgaag ttattcataa agatatcgaa ggcgcctttt
     1801 atcatatgag ctatgaacta gcagatggat caggcgtagt agaaattgcg acaacccgtc
     1861 cagaaacgat gttgggagat acggcgattg ctgttcatcc agaagacgag cgttatcaag
     1921 cgctcatcgg caaaaaagtg attctacctt tagtcaataa acaaatccca atcattgcag
     1981 atgaatatgt agatatggaa ttcgggacag gtgtagtaaa aatcactcca gcacatgatc
     2041 caaacgactt tgaggtaggt aatcgtcatg atcttccacg cgtaaatgtt atggatgatg
     2101 atggtacgat gaatgaacta gcaggaaaat atgctggaat ggaacgtttt gctgctcgta
     2161 aagcaatcgt gcaagatctg aaagaaatcg gtcgattgat taaaatcgat acgatgatcc
     2221 acagtgtggg tcattccgag cggacaggtg tcgttgttga acctcggctg tctactcaat
     2281 ggtttgtaaa aatggcgccg ttagcgaaaa aagccattga taaccaagaa acagatcaag
     2341 cagtggaatt cttccctcca agattcaatc aaacgttttt acgttggatg gaaaatgtcc
     2401 atgattgggt aatctcacga caattatggt ggggacatca aattcctgca tggtatcaca
     2461 aagaaacagg tgaaatgtat gtagggatgg aagcaccagc ggatagtgaa aactgggtac
     2521 aagatcctga tgttttagac acttggttta gttcagcttt gtggcctttc tcaacaatgg
     2581 gctggccaga tacggaaagc gaagattata agcgatactt ccctactagc actttagtaa
     2641 ccggttatga catcattttc ttctgggtaa gcagaatgat tttccaaagc ttggaattta
     2701 ctggcgaacg tccatttgag aatgtcttga ttcatggatt gattcgtgac gaacaagggc
     2761 ggaagatgag taaatcatta ggtaatggaa tcgatccgat ggaagtaatc gaaaagtatg
     2821 gagcagatgc tctccgctgg ttcttatcga atggttctgc accaggacaa gatgtccgct
     2881 tcagctatga aaaaatggat gcttcttgga actttatcaa caaaatctgg aatgcttcac
     2941 gtttcgttat catgaatatc gaaggaatga ccgtagaaga gatcgattta tctggagaaa
     3001 aatcagtggc tgaccggtgg atccttactc gtttgaatga aacgatcgaa cgagtaactg
     3061 agctatttga tcgttttgaa tttggtgaag caggtcgaca actgtataac tttatctggg
     3121 atgatttttg tgactggtac atcgaaatga gtaaagaagt actttatgga gatgatgctg
     3181 tttcaaaaca aaccacccgt agcatcttag tccatacact tgatcagatc ctacgtttat
     3241 tgcatccaat catgccattc gttacagaag aaatatggga acatattcct catcagggaa
     3301 attctctagt agtggcagaa tatcctgttg tacgtccaga gtttgatgat gaaacagctt
     3361 ctaaagggat ggaagtattg aaagaattga ttcgagccgt tcgaaatatt cgttcagaag
     3421 tgaatacacc attgtctaaa ccaattacgt tgctaattaa aacaaacgat gctaaaatcg
     3481 atcaattttt agttgaaaat acaagctata tcgaacgctt ctgtaatccc gaagagttga
     3541 cgatatcaag tgagatcact gcgccagatc tagcgatgtc agctgttctg acaggtgcag
     3601 aaatcttctt gccgttagca ggattgatca atattgaaga agaaatcaaa cgattagaaa
     3661 aagaactgga aaagtggaca agcgaagtaa aacgtgtcca aggaaaactt tctaatgaac
     3721 gttttgtttc taacgcgccg gaagaagtag tagaggcaga gcgtgcaaaa gaaaaagatt
     3781 atttagagaa gcaaacggct gtcaaagaac gaatcgaaag tttgcgtact attcaataag
     3841 aatagaaaaa gagagtgtga aatcagctag ctattactaa aagctagctg attgaacatc
     3901 gttacaggaa agagtttgag cctcactctt agcttcgatt cataaggaga tgtgacataa
     3961 attttgtcgc atctcttttt tagtctactc ttattatgta ggagaaggag ggaggtggat
     4021 catgtatcaa gagtcaataa agtagttgat aaaatcgttc ctctctctgt ttcttttctc
     4081 tttgtcatac ttttattttt cttatgtgtt ctatttgtta taataaaaga gaaatggggg
     4141 aattgttgat gacgattgaa gaagcaatag aatggatcca tagtcgatta ccatttggtt
     4201 cacgtcctgg ccttgaccga gtcgaagctt tattagaagt ggtagggcat ccagagagaa
     4261 aagtaaaaac gatccatatc gctggaacaa acggaaaagg ttctactgta gcttatttga
     4321 gaagcctatt agaagaggct gggctgacag taggtacatt tacttcacct tatatcgaaa
     4381 cgttcaatga acggatcgca atcaatggca aaagcattcc agacaatgat ttgatccagc
     4441 ttgtaaaaaa atatcagcca ctgatcaaac aacttgatca atttgaagca gtagctggta
     4501 ttactgaatt cgagacatta acagcaatgg catttgatta ttttttgatg aagaaagtgg
     4561 acgtagcgat tatcgaagtt ggcttaggcg gcttattgga ttctaccaat gtagctgtac
     4621 caatgctgac gggaataaca acgattggac ttgatcatac cgaaatactt ggagatacaa
     4681 ttgaagaaat cgctgcacaa aaagctggaa tcatcaaaca aggcgttcca gtagtaacag
     4741 gaaatattgc cccagaagct tttactgtca tccaacaaac agctaaagca aaaaatgcac
     4801 cgctttatgt atacggggaa aattataaaa taaactatct gcatcctgat gagatgtggg
     4861 gagaagtatt tgaatttttt aatgaaatag gaaagatccc tcaattaacc actccgcttt
     4921 tagggcaaca tcaagctgaa aatgcaggag tagccatcga attgtatttt gtttactgtc
     4981 aattggaaaa gctgcctttc cgagaaaaag atgttttgaa gggattgaag aaatctcgat
     5041 ggccaggaag aatggagcgt ttgagtaacg agccattagt tgttttagat ggtgcccata
     5101 atacgcatgc aatgaaccgg cttgttgaga atttgaaaaa agaattcaaa ggatatcaaa
     5161 tcaacatttt gttttccgcc ttacgaacta aaaatgttag agaaatgcta gaacgattgc
     5221 agcaagtacc aaatgtccat atttatttga cgacctttga acatccaaaa gccatcgaat
     5281 taagtgaagt atcagattta gcaagtgaga gagtatcaat cgtttcattg tggcaattcg
     5341 gattagcaga tatattggaa aagatgacaa atgaagacgt attagttgtg accggttcac
     5401 tttatttcat ttcagaagtt cgccatttat taatacaatt aggaggagat catgaagtat
     5461 aatgcagtga tttttgacat ggatggtttg ctgttcgaca cagagttagt gtattacgaa
     5521 gccacacaag tagtagcaga caagatgggt tttccttatg ataaagacct gtatttaaaa
     5581 ttcttaggtg tttcagatga agaagtatgg gcgaattatc atatgatttt ctcggaattt
     5641 ggcaaaacaa gtgtacaaag attcatagat gattcatacc aggaaacgct tgaacgtttt
     5701 tcgcaaggag cggtacaaat gaagcccggt gtgttggaac tcctctcttt tttagaagaa
     5761 catcagattc ccaaagttgt cgcttccagt aaccaacgag cagtcattga acttttattg
     5821 aagaagaaca atctgtttga acgatttgag acgattgttt ctgcagaaaa tgttcaacga
     5881 gcaaaaccag acccagaaat tttcttgtta gctaatcaga agctaggtac agcgaagaaa
     5941 gaaacattga ttttagaaga ttcacaaaat ggtattttag cagcttctgc ggctgagatc
     6001 ccggtagtca tggtaccaga tctgcttcca ccatcccaag aattgatcaa aaaaacagct
     6061 ggggttgttt cttctttaca tgaaatccct gcattgttaa aataaaaatc catctctttt
     6121 cgtatcttat tttagataag atagaaagga gatgtttttt tgggaaaacg attgatcaaa
     6181 gaagtaccta ctagctcttt accaagggaa cgtatggaaa tttatggcgc ggaagcttta
     6241 tcggatcagg agcttttggc aatcttactg cgtaccggtc aacaccctta tagtgtgatg
     6301 tcgattgctg gaaatctgtt aaaaacgttt ggaggactgg cctctttacg acaggctact
     6361 ttgcatgagt tagaagagat tcgagggata ggacgcgtga aagcaatcga aatcaaagcc
     6421 ttgatcgaat taggtcgacg aattcagacg gatcatgaaa gagcggctcc accggttcgt
     6481 accagctacg aattggccca gcaattgatt ctggaaatga aagatcacaa acaagaacat
     6541 ttggtttgta tttatttgga cacgaagaat caagtgatct taaaaaaaac agtgttcatc
     6601 ggttcgctga atcagtcagt cgctcatcct agagaaattt tccattatgc tgtacgttat
     6661 tgtgcagcaa ggatcgtttt agcgcataat catccttctg gtaatgttat tccatcgcag
     6721 caggatatga attttaccaa aaggatacaa aagtgtggag aaatgatggg gatcactgta
     6781 ctggaccact tgatcatcgg acgcaaacga tattttagtt tgagagaaga aggaatgatg
     6841 gaagagaagt aagttgcttt cttgcaaaaa aaatgaagca agtgtacaat tatattgtaa
     6901 ttttgtttgg gaatagaagt tgaaacatct gttgcacctc tatcgtacac gagcaatgtt
     6961 acaatttaaa ttagtgcttt tggcacgagg aggatattat tatggaaaac ggaacagtaa
     7021 aatggttcaa cgctgaaaaa ggatttggat ttatctcacg tgaagatgga agcgacgtat
     7081 tcgttcactt ctcagctatc caaggtgacg gattcaaaac attagaagaa ggtcaagcag
     7141 tgactttcga cgttgaagaa tcagaccgcg gcccacaagc tgcaaacgtt gtaaaagctt
     7201 aattttatct gtaaatcaaa ttgatggtag acatcaattt ttacgagaga acaaagcgag
     7261 taacaaaaat acctgccaca agagacaatt tctcttgtgg caggtatttt tttgtatgaa
     7321 aaagtaaata aatttaagag gctatgaaag agccaccttg tatcaaggaa taagaacaaa
     7381 caaaaatggc ttctcatgtt tttcatttgt tttctcttat attcaggaca aggtgctgat
     7441 ttcataacct cttatttatt cttcatctgg tgtcattacc attggcaaaa tcattggatg
     7501 tctttctgta cgttcgaata agaaaggctg aagcgcgcca gtcatcgctt ctttcaattt
     7561 atattctgaa cagtcaggtc cattcaaaca ttctcttaaa gcgtggaata agattcgctg
     7621 agcttcattg atcatttctc ctgattctcg catgtagaca aatccacgag agagaatatc
     7681 tggaccagcc aagatttctt ttttcttgat atccactgtc gcgacagcta atacaagacc
     7741 ttcttctgaa agcaggtgac ggtcacgtaa aacgacatta ccaatatcac cgatcccgct
     7801 accatcgata tacacatctc cagccacaaa atgcccagca ggacgcgcag aatcgctagt
     7861 taacgctaaa acatcgccat tttccatgat gaagcagttt tctgcaggaa cacctacatc
     7921 ttgtgctaga gaagcatgta ctttcaacat gcggaattct ccgtggacag gaataaaata
     7981 ttttggcttc atcaaacgaa gcattaattt ttgttcttct tgtccaccgt gaccggaagt
     8041 atggatgtta ttgatttttc cgtgaatcac ttctgcacca gcttcagaca gcaaattgat
     8101 caaatgattg acacttgttg tattaccagg aataggtgaa cttgaaaaga tcactgtatc
     8161 gtctggctgg atttggattt gacggtgagt accattcgcg atgcggctca acgcggccat
     8221 cgcttctcct tgagaacctg tacataggat catcgtttca ttggcaggca agcgattgat
     8281 ttcgtgtgca tctacaaatg tgccgtctgg aactttgata tatcctaaac gttgaccgtt
     8341 gacgatggca ttttccatgc ttcgtccgaa aacagcgatt ttacgaccag ttgccacagc
     8401 ggcatctgct gcttgctgca aacggaagat gttagaagca aagctggcaa aaatgatacg
     8461 tccttcgatt tttgaaaaga tcttcaaaat agaatgccca atcgtttttt ctgatttagt
     8521 aaatgttgga atctcagcat ttgtactatc agataaaagg cacaagactc cttcttcccc
     8581 gatacgagcc attttatgca agtttgctgg ttcgccaaca ggagtgaaat cgaatttgaa
     8641 gtctccagtc gctacgatat ttccagaagg tgttttcacc acgatcccta atgcatctgg
     8701 gatactatgg gtcgttctga aaaagctgac agaagtcttt ctaaaacgga tgatcgtatc
     8761 ttcattgatc tcatggagct ctgcatctcg aagcaagcca tgctcatcta atttgtttgt
     8821 gatcaacgct aatgcgagag gaccagcata gataggaata tttgcttgtt tcagcaagta
     8881 gggtattccg ccgatgtggt cttcgtgccc gtgagtaatc accaaggctt ttactttgtg
     8941 taagttttgt atgatgtagc tgtagtcggg aatgacatag tcaattccaa gtaaatcgtc
     9001 ttccgggaat ttgatcccgg catcaatcaa aatgatttca tcttggaatt gtaccccata
     9061 cgtatttttc ccgatttcac ctaatccacc aatagcaaag acgcctgtct cgttattttt
     9121 gatggaaagt ttcatattaa aactccacta atgagaaatc agcattttct ttttcgtact
     9181 ctagatgact tccttctaga agttgaacga attcaatatt gtatggggta ttttcttcca
     9241 cacgttggcg agcgtctaca tcgctttttg cctcaatgta aagagatttt gtttcttccc
     9301 gtttagggtt gcgtactttt gtttcttgat aataaacttt atagatcatt gttttgtctc
     9361 ctttatctgc gtaaagcagt gatttagcta gttgattttt ttagacaaca caaaacgagg
     9421 ggacaagcca cctggttttt catatgcttt gactagaata ctggattcga gtcaaagaga
     9481 caagcctcgt ttttgcttat ctgttcggat attttttcaa ccgtaccttc tgtataaaaa
     9541 atatacaaca attaattgtt attctagcat aaaacagcaa ataagtatag aaatcaacta
     9601 gattgaaaga aaaaatcaat gtttatcatc ccgctataat cataatgact tttttatgag
     9661 agaagtgttc tattaatagg ctcgttgtgc tatcattttt agtaatgaaa atgtaacaaa
     9721 agattaaaag gagatgagaa agtgaaacta atgtggcatt ataccatgcg ttataagaaa
     9781 ttcttgtttt tcaatttcat atgcgtattt ggttttattt tgattgaatt aggacttcct
     9841 acgattttag cacgtatgat cgatgtagga atccgcaatg atgattttga ttatgtgaag
     9901 caacaaggga tatttatgat cgttattact gtaatcggta ttattttgaa tatcttgtta
     9961 ggttattttg gttcgaggat tacgactaat atcgtagcag atattcgaga tgatttgttc
    10021 gaaaagatcc aaagttattc gcatcaggaa tacgaaaatc taggcgtatc ttcattgatc
    10081 actcgtacaa caaacgatgc ttatcagatc atgttgttcc tgcaaaatat tttacgtatc
    10141 ggttttatgg caccaatgat gttcattgtc agtttgtaca tggtcatgcg tacaagtgtg
    10201 acactcagtt tgtatgtagt aggtgcatta cctttactat tattggcagt cgtagcgatt
    10261 gctaaggtgt ctgaaccgtt atctaaaaag caacaaaaaa acttagataa aatcaacagt
    10321 atcttgcggg aaaatctttc aggattgcgt gtgattcgtg cattcgtcaa tgaaaagttt
    10381 gaagaaaaac gttttgctgt agtcaatgag aattatgctt ctagttcaaa aagcttattc
    10441 cgtttgatgg ctgttgcaca accgggtttt tatttcttat ttaatatcgt aatggtattg
    10501 atcatctgga gcggggcagt gcaaatcgat gcaggcgagc ttcgtgtagg agatttgatt
    10561 gcgtttatcg agtatatatt ccatgccttg ttctcattca tgttatttgc tagtgtgttc
    10621 atgatgtatc caagagcagc tgtttcagct ggacgtatcc aagaagccct agatgccaaa
    10681 ccttcgattg tagaagatcc aaatggtata tctgatacaa aaactaaagg gtatatcgaa
    10741 tttcaaaatg taacatttgc ttatccaggc cattcgcaag agccagtgat acgaaatgtc
    10801 agttttacag cctctccagg agagaccgtt gcttttattg gaagtaccgg tagcggaaaa
    10861 tctacgttga tccaattgat ccctcgcttt tatgatgtga gtgaaggaaa gatcttgatc
    10921 gatggagtag acgtgagaga ttatcggcta agcaagttgc gagataagat tgggtttatt
    10981 ccgcaaaaag cgctgctatt tactggaacg attgcagata atttgcgata cgggaaagaa
    11041 gatgctacgc aagaagaaat ggaacgtgca gctgagattg ctcaagcttc agaattcatt
    11101 tctcgaaaac cagatggatt taatgaacat ttatcagaag gtggttccaa tttttcaggt
    11161 ggacaaaaac aacgattggc aatagccaga gccatcatac gccgtccgga aatctatatt
    11221 tttgatgaca gcttttctgc attagattat caaacggatg cgaagttacg tgctcgtctg
    11281 aaaaaagaaa cgacagaatc tactgtattg atcgtagcac aacgtgtggg aacgatcatg
    11341 catgcagata agatcatcgt attaaatgaa ggagaagtcg tcggtatggg gacgcatcgg
    11401 gagttgttgg aaaaatgccc gatttattat gatattgccg cttctcaatt atcaaaggag
    11461 gaattagcat gaaaaaaacc ttttcttcct taaaacgttt agctcgatat atcagaccgt
    11521 atcgtgagac ttttctttta gtgatcctat ttacagttct gacagtggct ttcaacgtgg
    11581 ctttgccata tgttacagga cttcctacta cagaaatcag ccgtaatatt gccaatggcg
    11641 aatcaatcaa tttttcttat atattcaaat gtctcatttg gatcacagtt gttggaatag
    11701 ggtattgtat ttcgcaatta ctttctggtg ttttgatgac aaatgtcgtt caaagtgcca
    11761 tgcatgattt gagaagagac atagaagaaa aaatcaatcg tcttccggtt tcatatttcg
    11821 ataaaaacca gcaaggaaat attttgtctc gtgtgacgaa tgatgtagat gcagtcagcg
    11881 gtgctttaca acaagctttt attggtatcg taaatgcgat attgggaatt acgttggctg
    11941 cagcaatgat gttttatatc cagccgatga tggcgctgat ctctatgatc atgattccaa
    12001 catcgatctg gatctcaaaa cgagtgatca atgcttcgca gaaatatttt caaagcatgc
    12061 agaattctct tggtgagcta aacggctacg tccaagaaaa tatgacaggt tttagtgtat
    12121 tgaaagtata tggacgcgaa aaagaaacat tggaaggatt caaaaaagtt aatcacagct
    12181 tgaaatactt tgggtttcgt gcagcattca tttctggctt gatgatgcct ctagttcaat
    12241 taactgccta tgctacttat attggtatgg ctgttctagg aagcttttat gttattactg
    12301 gggcgatcgt tgtcggtcaa ctacaagcat tcatccaata tatttggcaa atcagtcagc
    12361 caatggggaa cgtgacacaa ttgtcttcag ttttgcaaag tgcatcagcg gcaacgaaac
    12421 gtgtattcga gattttagat gagccagaag aaaaagaaaa caaagtagat gcttcactgc
    12481 cagaaacgat tttaggagat gtgacattcg atcatgtcga ttttgcatat gatccgaaga
    12541 aaccattaat ccaagatttg aattttgaag tgaaagctgg tcaaaccgta gctgtggtcg
    12601 gtccgaccgg tgccggcaag acgacattga tcaatctatt gatgcgtttt tatgacgtag
    12661 acaaaggagc aataaggatt gatggaatcg acaccaaatc aatgagtcgc agtgatgtgc
    12721 gttcgctatt tggtatggtc ttgcaagatg catggcttta tcaaggaacg attgcagata
    12781 atatccgctt cggaaagctt gatgcgacag attatgaagt agttgatgct gcgaaaacag
    12841 caaacgtaga tcattttatc cgaacgatgc cagatggata tgatatggcc atcaattctg
    12901 agggagacaa tgtgtcttta ggacaaaaac agttgctgac gattgcccga gcagtggtct
    12961 ccgatcctaa aatcttgatt ttagatgaag caacaagttc agtagacacc cgtttagaag
    13021 cgttgattca aaaagcaatg gatcgcgtga tggagggacg gacaagtttc gtcatcgctc
    13081 accgtttatc gacaatccgt gatgcagatc tgattctagt gatggatcaa ggtcagatca
    13141 ttgaaaaagg aacgcatgag agtttattgg cacaaggtgg attttatgaa aaactgtata
    13201 acagccaatt tgctgaggaa gcagaataag tcatataaga ataaaacagt ttaaaactta
    13261 ttttttgttc tctattcttg ataatggtaa agtaaagtta tcaagaaaag tgaggacacg
    13321 gagatggaac agtttttaaa tgaaaaaatt actgaagcca aaagagaagt tgatcgttta
    13381 acgcacgtct gtcagacgaa gccttttgat tcagctgatt tttttgaaaa tgagtttcaa
    13441 atcgaacgct tggcagcaca aatagaagtg tacgaattaa ttttaaaaaa agtaatagaa
    13501 gaaaatatag atgtacaata aaaacaggta aaacgtcagt taccagatac tgtaaataga
    13561 catctggcct gagcaggttt tacctgtttt ttttcttatg aaataaatat atgctatgat
    13621 acctttattc aataggatta gtttctacaa aaaatgaaag gagaaaaata aatatggaac
    13681 ggtttatagc atcaaatgcc acagtgattg gtgatgtgac attgtcagaa gatgtgacga
    13741 tctggtatca agctgtttta cgcggggaca gtaattggat aaaaatagga caaagaacga
    13801 atatccaaga tggtacaatc atccatgtcg atcatgatgc acctgtagat attgcggaga
    13861 atgtgacggt aggacatcag tgcatgctac atggctgtac aatcgaaaaa ggggcgctga
    13921 tcgggatggg gaccacgata ttgaatcatg cggttattgg tgagaatagt ttgattggtg
    13981 ctggatcatt agtaacagaa ggaaaagtta ttcctcctaa tgtcttggct tttggtcgcc
    14041 cagctaaagt gatccgtcct ttgacaaaag aagaaataca aaaaaacaaa gaaaacatcc
    14101 agcattatgt cgagataggt cggatgcatg cgcaaggaaa attcaaggag caaagatagg
    14161 cagtagcatc cataaaataa aaaaggatgg tcagtttgag ttgaccatcc ttttttattt
    14221 ggtttagata agagagctta aaaaagcttc ctttgaaaca gattaattta atgaagaact
    14281 gttttccatg acttcatcga tcaatccata tgcttttgct tgttcagctg tcatatagtt
    14341 gtcacgatct gtatcttttt caatcacttc aagcggttgt ccagtgcgtt cagctaaaat
    14401 tttgtttaag cgttcgcgtg tttgcaaaat atggcgtgcc gcaatttcga tttcagttgc
    14461 ttgcccttga gcgccgccaa gaggttgatg gatcatgatc tcagcatttg gtaaagcaaa
    14521 tcgtttacct tttgtaccag ctgtcagcaa gaagcttccc attgaagcag ccatacccat
    14581 aacaattgtt tggacatctg ctttgacaaa gttcattgtg tcgtaaatag ccatacctgc
    14641 tgtaactgaa ccaccaggag aattgatata aagatagata tctttttcag aatcttgagc
    14701 gtctaagaaa agcaattggg cgatgatcga attagcaaga tcatctgtta cttggccgct
    14761 taacatgatg atacggtctt tcaataaacg tgagtagata tcgtaagctc tttcacctcg
    14821 tgaagattgt tcaatgactg taggaattaa attcataata gagttcctcc ttgatttcgt
    14881 ttagaaatag tgtaccataa tttagtttct aatacctaaa atgtagtata catttttggt
    14941 caaaaaaggt caaataaaaa acttaggcaa attagtcgca taaacccgct ttatggtatg
    15001 ataagcagag aaacaaaaca aggaggaaca acagattgat agttggtatt actggcctta
    15061 tcggagtgct gacaggtgct gtagttgtct atatcggcat gatactgcaa atgcgtcact
    15121 tggaaaataa agaacagcgt agacgtgaat tggaattgaa agtcaaagaa atcgagacgc
    15181 tgaatcaatt aaataaaaag atcaacgaga tcttgcaaaa acggacaacg ctgatggaag
    15241 aatatgtcag ctttgatgca tttgatgatt gttatatcac gattgacgat tttgcgtatc
    15301 tccaatcatt ttcagcgcaa aatagttttt atctgccaaa ttatatttta gacgagctat
    15361 ttaagaatat ctcccatcga aaagtgattt tatctccaga tgaaacaatg aagattggtg
    15421 gatatgctta taaaggcgga cgcgtgattt tagagaattt tacggatagt ctgactgaaa
    15481 tgatcaatga aaaaaaagct cagttgaaga ctatgacgcg ccagccgatt ggtttcttct
    15541 cgaaagatga tgtttggagc tgatattttg aagcattgcc gttgcacatg agagcgtggt
    15601 aatgctttct ttttttctta atgatacaat agaactgaac ggagtgaaga aagatgttac
    15661 aaatctatgc ccatcgcgga agcagcggca cccacccaga aaatacgctt cctgcatttg
    15721 cagaagctgt acgagtaggt gcagatggta tcgaactaga tgtccatcta agcaaagatg
    15781 gttatttgat cgttatgcac gacgaagaag tggaccggac gactaacggt aaagggctga
    15841 ttcgagaaaa aacattggaa gaactcaaaa aattgaatgc agggggcaaa ttcagcaaag
    15901 aatttccagc agctaaagtt cctgaattga gagaagtgtt aggactgctc ctccaaaaaa
    15961 attaccgagg gatgctaatg attgaaatta aaactgatca gtatgaatat cccggtatcg
    16021 aagaaaaatt agccgaaaca ttaaaaagta gagattggcc ttttcaacat gcatattgca
    16081 gctttcattt ggattctttg gaaaaaatcg cattactgga accggagata cagctggact
    16141 tattgatgag tacttctgat aaaaagatcc aactagcagc agaaacccct tatatagaag
    16201 gagtacatcc aaaaaatact tggttgttcg aacatccagt ccaggcagct gattttccaa
    16261 aagcaattcg tgcatggacg attaacgatg aagaagaaat ggccaaatgt caagaactca
    16321 aaatagatgg aataataact gattttccgg agaaagctct tcggtggaaa caatcaacga
    16381 aaagtaggta aaatatggaa aaagtattag tgatcgtcgg tccgacagca gtcggtaaaa
    16441 cagcgctaag tgtggaactc gcacaactat ttggaggaga gatcatcagt ggtgattcat
    16501 tgcaaatcta taaaaatctg gatatcggta cagcaaaaat caaagaagaa gagatgaata
    16561 atgttccgca tcatttgatt gatgtgatcg aacctactca acgttactca gccgctgatt
    16621 ttcagcattc aggtcgacaa ttgattaccg agattgcgaa ccgagggcac ctacctatca
    16681 tagctggtgg tacgggtctc tatatccaat cccttctata tgattatcag ttaggagcga
    16741 cagaagaagc gaatcctgag attagaaaaa agtatgaaac gttagctgaa acaattggtt
    16801 cccaagcatt atggaatcag ttgaaagaaa aagacccggc agctgctgag aaaatccact
    16861 ggaacaatca acgcaaagtg ataagagctc tggaagtttt agagacgaca ggacatagta
    16921 tcacggcacc taaagaagag ccaaagcaat tgtatgatta cctcatgatt ggattagata
    16981 cgaagcgtag tattctttat gaacgaatca atcaaagagt cgatttgatg ctagcagaag
    17041 gtttagtagc agaagcgcgt aaggtgtatg agcttggaga agtccaggcc agtcaaggta
    17101 ttggatataa agaatttttc ccttattttg aagggaaaga aacattggaa gaagcaacag
    17161 aacaagtcaa actacattcg agaagatacg cgaaacgtca gttgacgtgg tttcgtaacc
    17221 gtttggatgc acagtggttt gatcttataa gtgagccaaa tcaaaaaagt cagattgagc
    17281 aaacaatcaa aaaatggctg gaggcggaat aaaatggaag aaaaagtgat catcgtaggg
    17341 gtagaaaccg aagcaaacca gcgttacttt tcagagtcaa tggaagagct agttcagctg
    17401 acgaataccg catcaggaga agtcgtattc acaattacac aaaagagacc acaagtggat
    17461 cggcaaacga ttatcggtaa agggaaatta caagaattag ttcaacaagc ggatgcgcat
    17521 gaagcggatt tgatcatctt taatcatgag ctaactccga gacagagcca actgattacc
    17581 gatgctgttg gtcttccggt aattgatcgc gtgcagctta tcttagatat ttttgcgatg
    17641 cgtgctcgtt cgaaagaagg gaagctgcag gttgaacttg ctcagttaga atacctgctg
    17701 cctcgtttag tagggcaagg gaaaacatta tctcgtcttg gcggtgggat cggaacaaga
    17761 gggccaggtg aaacaaagtt agaaacagac cgccgacata tacggaataa aatttttgct
    17821 gttaagaaag aattgaaaga agtagaagct catcgagagc gcaatcgcca aaaaagaaaa
    17881 aatagcgaga tttttcagat tggacttatt ggctacacga atgccgggaa gtcaactatc
    17941 ttgaatctat tgacacaagc ggatacttat tcgaaagatc aattgtttgc aacgttagat
    18001 ccattgacta agagatggcg ttttgctgaa ggatttgaga ttactgtaac ggataccgtc
    18061 gggttcatcc aagacctgcc tacgcaactg atcgatgctt tccattctac attagaagaa
    18121 agtcaaaata tggatctttt gctacatgta gtcgatgcta gttctccaga tcgtatttta
    18181 caggagcaga ctgtcttaaa attgatggat gaactgaata tgaaagaaat gccgatactg
    18241 actgtttaca ataaggcaga ccagatcgac ccagcgatgt tcacgcctac gctattccca
    18301 aatgtattga tttctactca gacgaaagaa ggtaagatgg cgttgatcga agcaatcaaa
    18361 cgtcagctga tggaactaat gaccccttat acactttctg tgccaagcaa cgaaggacag
    18421 aaattaagcg agttgagaag acaaacaatg gttttggacg aagcgtacat ggaagaaaca
    18481 aacgaatatg aagttaaagg gtttgcaaaa accaattcaa aatggttaag aaaaaaccaa
    18541 taaaatttta tttttttaa
//
LOCUS       DAYYCW010000057        18146 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000057 DAYYCW010000000
VERSION     DAYYCW010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.057"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(50..1558)
                     /locus_tag="ACHAYA_001744"
     CDS             complement(50..1558)
                     /locus_tag="ACHAYA_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM8605541.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1717..1908
                     /locus_tag="ACHAYA_001745"
     CDS             1717..1908
                     /locus_tag="ACHAYA_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605542.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2111..3028
                     /locus_tag="ACHAYA_001746"
     CDS             2111..3028
                     /locus_tag="ACHAYA_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM8605543.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3021..4094
                     /locus_tag="ACHAYA_001747"
     CDS             3021..4094
                     /locus_tag="ACHAYA_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605544.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4242..4760
                     /locus_tag="ACHAYA_001748"
     CDS             4242..4760
                     /locus_tag="ACHAYA_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605545.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4741..5133
                     /locus_tag="ACHAYA_001749"
     CDS             4741..5133
                     /locus_tag="ACHAYA_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM8605546.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5209..6144
                     /locus_tag="ACHAYA_001750"
     CDS             5209..6144
                     /locus_tag="ACHAYA_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8605547.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6277..7056
                     /locus_tag="ACHAYA_001751"
     CDS             6277..7056
                     /locus_tag="ACHAYA_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8605548.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7049..7366
                     /locus_tag="ACHAYA_001752"
     CDS             7049..7366
                     /locus_tag="ACHAYA_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8605549.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7610..9220
                     /locus_tag="ACHAYA_001753"
     CDS             7610..9220
                     /locus_tag="ACHAYA_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8605550.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9377..10225
                     /locus_tag="ACHAYA_001754"
     CDS             9377..10225
                     /locus_tag="ACHAYA_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8605551.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            10456..10935
                     /locus_tag="ACHAYA_001755"
     CDS             10456..10935
                     /locus_tag="ACHAYA_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM8605552.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            10968..13196
                     /locus_tag="ACHAYA_001756"
     CDS             10968..13196
                     /locus_tag="ACHAYA_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8605553.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            13325..14968
                     /locus_tag="ACHAYA_001757"
     CDS             13325..14968
                     /locus_tag="ACHAYA_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8605554.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(15041..15586)
                     /locus_tag="ACHAYA_001758"
     CDS             complement(15041..15586)
                     /locus_tag="ACHAYA_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM8605555.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(15609..16094)
                     /locus_tag="ACHAYA_001759"
     CDS             complement(15609..16094)
                     /locus_tag="ACHAYA_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8605556.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(16084..16896)
                     /locus_tag="ACHAYA_001760"
     CDS             complement(16084..16896)
                     /locus_tag="ACHAYA_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM8605557.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(17035..>18146)
                     /locus_tag="ACHAYA_001761"
     CDS             complement(17035..>18146)
                     /locus_tag="ACHAYA_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8605558.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         5894 a         3303 c         3631 g         5318 t
ORIGIN      
        1 atcgattaag ctaatagttt cgttttcaac atttataagt aaactttttc tattggtaat
       61 atagattttc ggaagggtag acaggtgcgc aggtaatatc gattcctagt tttttcaatg
      121 tttgttcatc atttttattg accatcacag tagagtgggc ttgtgctccc tcaagttcag
      181 ctagtttatc ataagcaact tgagcgattg gattcgttac tgcactgatt gataaagcga
      241 tcaagacttc attgatgctt agtgcatcca aagaggaatg aagtgtcttt gatttcaatc
      301 cttgtattgt ttcaagaact aatggtgaca ataaaggaat gtcatctgcg atatgggcta
      361 aatgttttac tgcatttaag ataactgccg ctgacgcatc catcaatgaa cttgtacgcc
      421 cggtaatgat gacttcatct ggtaattcga ttgcaatcac agcagaagga tcttttgtag
      481 gactttgcgc ttgaatctta tctgcatagt catgggcagg gactaccacg tttcggtctt
      541 caggacgtaa accgacttct tccataatca gtttgattcg attaactgct tcttcgtcga
      601 tcaatccttt tttgaagtcg cattctgttt ggaaataacg acggatgatt tcttgtttag
      661 aggcttcttg gacaacggta tcatccacga tcccaaatcc tacacgattc acacccatgt
      721 cagtaggaga ttgataaaca gattctttac cagtaatttt ttcaataata cgtttgatta
      781 caggaaatgt ctcaacatcc cgattatagt tcacagctac tttattgtag gcatcaaagt
      841 ggaaggaatc gatcatattg acatctttta gatcgactgt ggctgcttca taagcaatgt
      901 tcagcggatg tttcaacgga acattccata ctgggaaagt ttcgaacttg gagtaaccag
      961 ctgcatgtcc tttttggctt tcatgataca gttggttcaa ggaagtagcg agctttccac
     1021 ttcctgggcc aggagctgtc actacaacga ttggtttagt tgtttcaata tactcatttt
     1081 ttccaaaacc atcttctgag acgatttttt ctacgttgac tggatagtct tcgatttctg
     1141 agtgtttata gaccttgatg tttcgtcttt ctagtttatt gataaagact ttcgtggcag
     1201 gctgtccact atagcgagtg ataacgacac tgttgattaa taagccataa ccacgtaatt
     1261 catcgattaa ccgcaagata tccatatcat aagtaatccc ataatcacca cggatcttat
     1321 tacgttcaat atctccagca tagacgcaaa tcagtatttc agcttgatct ttcaattttt
     1381 gcagcaattt gattttcgca tcttcttcaa atcctggaag tacacgtttc gcatgcttgt
     1441 cgtcaactag cttcccacca aattctaaat ataatttgtc atagtggttc actcgttcca
     1501 aaatatacgc tgactgctct tcaatatatt tttctgaatc aaaaccaatt tttttcacta
     1561 ggttccctcc ttaagtacta aagcattttt tcattttttt gaggattcgt caactggaag
     1621 agcgaaattc ctcaaaaaaa caaagaaaat ggtttaaacc aacttgtcta gatagttatt
     1681 cattggtatg atagtttttg ataaggaagg tgctacatga aaaaaagaca gaaaaagaaa
     1741 aacgcatata aacactatat ccgttcgatt tttaccggtt atgaaagaat gcttgaagat
     1801 cccgaattgg aacaattgac gtttacttat ctaaacgaag aaacgcagct aactcgtgat
     1861 gagcatcagc gcattcattt cacaacaaga gatctgccta gtaaataaaa aataagataa
     1921 aggtcatcgg attcgattct gtcctatcag atggaaagac tagttgatcg aaaaaaaggc
     1981 tgtaccaaat tttggtgcag cctgttttat ggagcattgc ccgctctttt ttataacaat
     2041 tttttacaga aaatggtact attgaagtat taataagtga aaaacagatg agtaagagga
     2101 ggcaagtagt atgaaagagc cagatacaaa tgcaaattat gagccagacg aactagagga
     2161 gacgaatgcg gatcaggata caggacaaga atcgtcttct ttttacgata tcaaagaaaa
     2221 agcaagcatg aatctttccc aattattaga agacttacag gcttttgaaa aagctgtccg
     2281 cgaagaagat atccctgaga tctaccgttt atacaatggc cgattgaatg aagaattaaa
     2341 aagaagctca aatgaaaatc acgagatcga tcaattgctg atgcgtaaga tccatgatcg
     2401 ttttttacag gaatttcctt ttatggaaca agtagaaaac atctcgccaa cgatgagcta
     2461 ttacaaaatc ggcacttatt accatgatcg gcccacggtc ggtattgatg caagtctgcc
     2521 agaaattttt gttttgccta agatcgatga agagtgggag aaatattccc aaccggagtc
     2581 aacggactat gacaaaaaaa tcaatgagct agacgcaaag gtgatcactg ctcagacaga
     2641 aattgaacgt ttagatgaac aaataaaaga aatcaatcga caaatgactg atttgaacga
     2701 caataaagga ttcttgaatc gtaaaaaaat cgaggaagaa attcaagaat tagagaagaa
     2761 gaagcaagta ctatctaatg aaaaattagg ctggcttcct tatattgaga cgcctgaaac
     2821 aatccagcag caaaaagaag cattgaagca agaagcaaga gcagaccagc ttcgtgcagc
     2881 catcgtagaa aaagaacaaa ggcaaatcag acgctatttc ggtagcaaag agggattcgg
     2941 tcaagcaatc catgaattct tgatgaatta tcttggcaat gagaatccta ataatcaatc
     3001 taatgaagga ggttccgagt atgaatgaac acgatgaagc agaatggaca aaaaatgaag
     3061 aacaaacaga agatcatgaa gaaacgaaca cttcattgga agaacaaaca agtaatcctc
     3121 tggatgacac agaagaaatc cctatgctag aggaaacaaa aatacacgaa aaagaaaaag
     3181 taactccgac gaatacgaat actgcagaaa acacccatgc ttacgaaata actgaaaatc
     3241 ccgggtattt agaaggaaaa gagcaagatc atcaagatat cgtcagtttg attgaaacag
     3301 cggaagcaga attagcggat ttacgtatga gaaaagattt gatcgaatca gattttccac
     3361 aattattaga atcatatgat cgttttatat tgaatgaaga accaatctcc acgatcggaa
     3421 agacaagcct gatcgaatac ttcacgtatg aattactgaa accgttgaat gatattggtc
     3481 tggaacggtc aaattcagct tatgacacat ggaaaacaag acatttttcg atcaattata
     3541 cgttgaatgg acaaaacgaa ttagtctttt cctttgtatt accgaccatc gatcaacgat
     3601 accaagtgga atcgatgagt ctcctaaacg tatcaccaga gacaatggaa atcaatgtcc
     3661 aagatgaccg ggtgctttct ttgatccgac actggtcagt ggatcgagta ttctctgcag
     3721 ggcagatcac gatattcaat cacaaattga atcaattatt agcccatgca agaacacttg
     3781 gttttaccgt taatcagaca ttactagaca atacgaagcc acttcgtttg aatcttcaga
     3841 gcgaatttga attgacgaat gaagtattgg atgatatttt tatcgtggca atgaaaaatc
     3901 catcctacga tatggaaaag atcaacgaaa ctacttatca agtacgatta gataaaggac
     3961 agtctttaac gataacaaca aacgaaaaag atcaaacagt cttgtcgatc tcatctggag
     4021 actacccacg atctgtcatt gatttcttta tcaattacga attcttggtc ccattaatgg
     4081 tgagaaagtc ctgattggat aagttggatt accagatgtc attcagataa ttctgttcca
     4141 aaggacttgc ggcagttatc cagaataatt gtcacaagtc ttttctgtat gtactaaatg
     4201 gacgtgaata acttgctgat gaaggaggga tttcattagg aatgtccaca aataaaatag
     4261 cacagcttag cttattgtct gctgcttgtg tagcaggtag gatcgctttt caatttattc
     4321 cgaatttcca gccaatgact gctatttttt tatttatagt actttactta agcttaaaag
     4381 atgcgttaat tgtcatgagt ctttctattg cgatcagcag tttttatttt ggtgttggac
     4441 catgggtcat cggccaatgg atcagctata ctgttgttct gagtttgttt tcaatcgtct
     4501 gcttgagaaa aacagtgcaa aggaatctat ggttaaaggg agtctgtttt tttctggcgg
     4561 gcatggttta cggaataggt atgaccgttt ttgatacgct gttatatcgt ttgcctcaac
     4621 cttggatcta ttaccttcaa ggggtgtcgt ttgatttgat gcacagtata ggcaatgttg
     4681 tgttctttct tgtttttctg ccagtcgtca aacgttttta caatcattca ggaggaaaaa
     4741 atgaaaaaaa taatttatag tttgcttgca atgagtgtca gtatactcat tttgacaagt
     4801 tgcagtacaa cctatacaca atcggcttcc aaccaaaagg aggaagtaaa aacatcgcaa
     4861 aaagcaacaa tcacgttaca agaaaatggt caaaactttt cggaaaaaga agtgttcttc
     4921 aaaaaaggcg atgatttact gagtattttg aaacgaaatt ttgaagtcaa agaagataac
     4981 ggcttcatca cttcgcttga agggcactca caggatgaga aaaatcaact ctattggatg
     5041 tttactgtgg acgggaaaag tgctactact ggtgccaaag agatcaaatt gaatgatgga
     5101 caatcggtca ttttcaattt gtctaaacta taagtttcat aaaaaactgt taaagggcga
     5161 atattgccat aattcagtga gttcagttga tgaaagaagg aaaataacat ggaaaatttt
     5221 aaaaaaagcg gaatgttttc cgtaattgct gctctagggg ctaatattct agttgctatc
     5281 agcaaatttg tcggctatgc aatcagtggc agtgccgcca tgctaaacga aagcatccac
     5341 agtatcgttg attgcagcaa tcagattttg ctgttattcg gtgataaaca agccaataaa
     5401 ggacagagcg agttgcatca atttggtgaa ggacgagcaa aatacttttt cagtacaatc
     5461 gtcgcaatga tgcttttctt cggcggtggg gcacttgggg tgatggaagc ttttgaaaag
     5521 ttgacgcatc catcccatga agtgggtaat ccgatcattg ttatagcaat actgttattt
     5581 ggtttggtaa tcgaaggaag ttcgttacgc gtggcaatga aagaaatcaa agaattgaat
     5641 actgaaaaac tttccacgat gcgctttttg cgcgaaagtc gacacagtga aatactgatt
     5701 atttttacgg aagacttgtg tgctgtaatc ggtttggtct tggctttagc aggaacaata
     5761 ttgacttatg caaccgggat tgctgcattt gatgcgatca gcggattgct gatcggtttc
     5821 ttgctgatgg gagccgccat tttcctagca aaagaatttt atagtctgat catcggtgaa
     5881 agcgtgacag caagcgattt atcaaaaatc aagttagctt ttgaacgtcc tgagatcagt
     5941 cggctgatcg atataaaaac agttcattta agtccaacgg agattttagt tgcagcaaaa
     6001 gcagatatcg ttggtaacaa agagaatgaa gcttactcgg tcatcaatga tattgaaaaa
     6061 aatatgcgtc agtctcttcc tgacaagaag atgtatatat acattgaaac agataaatat
     6121 gacccaaatt attctcgtaa ataaaacgtt tattcatgaa ttgaagcaaa aatgagaaaa
     6181 atataatctt gtttttaaat aaaacggcta caatttagcg aatttatgtt acaatgagtc
     6241 cgctgtttat ttgttgtaat taaaggagat attttaatga acaaaaagaa aagtgcgcca
     6301 tggcttcaag ctttgcatgc agtgatgcca ctttgtataa gctatatacc agtaggatta
     6361 gcttgcggag tattgttgca aaaagttgga tttgatcctc tcttatcagg actgttatcg
     6421 attctggtct tttccggtgg cgcgcagttt ttagttgcat ctatgcttac gacgcaagca
     6481 tcatttgcaa cgacattatt gatggttttc tttttagaat tacgctatac attattagga
     6541 gcaagtcttg ccggttttat gaaaaaagaa aagcggacgt ttttggctgt cttttctcaa
     6601 tcactgaatg atgaaaacta tgcggtgaac tacttaaagt tttcaactga cccttcctgg
     6661 aataaaagaa aggctttata tgtcaattgg ttctcaatga ctgcatgggc cggtagcaat
     6721 atgatcggta attttttcgg ctcggtgatt cgagtagatg ctgaccttgt ccattttgct
     6781 ttgacggcca tgttcatttt catgtttatc atgcagatga aaagcgcact actgatcttt
     6841 acaggtttat tctcaggcgt attaggggtc gtattcatgg tattattcca aaatactttc
     6901 ggattgattg cagcaacagt cattgcttca tttgccggat ttatgctgga gaaggcattc
     6961 aaaaaagaaa aagctaagaa actaagagac cgcggtaaag atgtcaatga gccgctcttc
     7021 aacttcccgg aacaggaggc caatcaccat gactaaacta tatttgctac ttttagttgc
     7081 ttgtctgttc atcgtggcat atatcccgcg cttgttcccg atgctttatt ttactcatcg
     7141 aaaagtgcca tcctggttca gtgactggat gaaatatgta ccggtggcat tatttgcggc
     7201 gttagctttt aaagatgttt tcattactca tgaacatctg gatattgctt ggaatatcaa
     7261 aatagctgca atgattctag tagcaggcgt ggcttacaaa acacgttcga tggcgttgtc
     7321 agttttgaca ggacttgcgt ctgtcttttt gctgtctatg ctttaaactg ttttatttta
     7381 ttaaaaacag agattgcgac ataagttgtt caaactgatc tatccgaact gcatttgcaa
     7441 cagtcaattc tggatgcaag tttattcgta gggataaaaa agattagtgt tggcttatga
     7501 tgcaagctct tttttatttt aggaattaga gagggatttg tccttctatg attttttgct
     7561 ataataggga agatgtttat tttatgaaag aatagttgag ggaatttaaa tgaataaaaa
     7621 attgatgcaa ggaacatttt ggctaacctt tgctaatctt ctatgtaaag ttttaggggt
     7681 agtctatttg attccctggc taagtatgat ggggaacaat caagatggta tgcttgctac
     7741 gacattgtat aatgttggct atcttcctta tggattgttc ctgatgcttg gtacagtcgg
     7801 atttccaaat gcgattgcaa aaaaagtagc tgtagccaca aaagaaggag ataacgctgc
     7861 ttgtcggctg atttttagaa gtacgatcaa tatcatgttt gtcatcggta tcgcctctgc
     7921 tgcactgatg tatttatttg ctcctttact agccggaatc agtccgattg ctaacgttaa
     7981 taatgggata ctagctatca gaagtttatg cccatctttg attgctatcc caattcttag
     8041 tgcaatgaga ggatatttcc aaggaaaaaa tgatctgcgg ccttatggaa cttctttgat
     8101 tatcgaacag gctgtgcgag tgatcgttat cttggctggt acttattact tgcgcgtatt
     8161 gactgacgga accatattag aagctgtatt gatcagtaca gtcgcttctt tcttcggtgg
     8221 gttagctgcg attatccata tgtatatcgt cggacgcaga aaagacttct tcgaacttaa
     8281 agattttttg atttccagcc gttatttcga aagagaaaac cgatctgcct ctgtctctat
     8341 cattcgggaa acactaccgt ttatttttgt cggctcagtc attactatcg ttcaaatgat
     8401 cgaccaagta acgatgaaac cactgcttca ctttttccgt ccagagatcg cagatcagca
     8461 actggaattc ttattcagcc gtgcttctgt caatccaaat aaattgacat tgatcctgat
     8521 ctccatggta ggaacagtag ctataagcag cttgccaata ttaagcacga tgaagaaaaa
     8581 agatcggata caaatcgaaa aaacagtagg agatagtttt tcgattgcgt tattgattct
     8641 tcttccttca ttgactggaa tgtctttatt agctggacca ctatacacat tgttttttgg
     8701 ctatgatcct gaaagtgtcg gatatttcca aatggctttg ctagcttctt tgtttttctc
     8761 tttattcacg atcctatcta cgatgcttca atctttgaat caccatcgtt ttgcgatccg
     8821 attaactgta gaagcaatta tcctgaaagt catcttccaa gtaatcggat taggattagt
     8881 tggcggatac gggatgagct tatcaaatgg cttagcattc ggtattgtct ttgttcgcgg
     8941 ctatcacttt atgtgcaaag aataccgaat agcaccttta gcaaagatca gcaatttttt
     9001 cttaaaaact ttccgctcca ccctgattat gcttgcagta tgttttgccg ttttcttttt
     9061 gctgaatcag cgattgtcaa tggaatcaaa aagctatgca atgatttatt gtgtggctgt
     9121 aggaagtatt ggcggcttag tatttttatt ttctcaattc ggaaaaaatg ggtttcgaat
     9181 gttgaaaaat gttaaacatc atagcagaag caaagaatag gttaactaat cgttttttgc
     9241 tgtcacttga aaaacggcaa ctcgtatgat tacgcttgtc gtttttctat tttccaattt
     9301 tagtttcata tctaatctta cttatgatat agttataagg tctggaaaaa ttctttgaaa
     9361 cgaggaaatt aaacacatga aacttgcagt agttacagat agttcggcat atctgccgga
     9421 acgtatcaag aaccatcccg acttattcgt tattccaatc cctttgatta tggatgggaa
     9481 aatttataac gaaggaatcg atatcgaagc ggatgaatat tatggacttt taaaaaacag
     9541 taaagatttt cctactactt cacaacctgt cgtaggggaa gttttgggtt tatatgagga
     9601 attgaaagag aaagggtatg acacgatttt aagtatccat ctttcttcag ggatttctgg
     9661 ctttgtcaac acattatttg cgatgaaaga tgacatcagc ggagtaacgg tcattcctta
     9721 tgactctaag atcactagta tgccgatggg acacatggta gaagctgcct tagacttaaa
     9781 tgatgaaggt cagtcccttg atgaaatact tgcacatatc gatcgaatcc gagataatac
     9841 ctacgcttat ctgattgtag atgatttgaa caatttagta cgaggaggac gtttgacaaa
     9901 tggtgcagcg ctgattggca gcttattaaa aatcaaaccg atcctgacat tttctgaagg
     9961 gaaaattgtt ttattcgaaa aaatccgttc aagtaaaaaa gcatttgctc gagctgaaga
    10021 aattattggt agaagaaata acgaaataca accgccagtc aaactttacg tgatacatgc
    10081 aaataattta gaagtagcga ttgcggagaa agagaaattg cagaaaaaat atccaaatgc
    10141 agaaatcgaa attggtcatt tcggaccagt cattggtaca catttaggtg aaaaagccat
    10201 cggcattgcc atttcagcgc aataattcct tttattcaaa aaacatacca aaaatattcc
    10261 aacatcatct tggactttct acatgttact aatgagcgtg taaaagaacg agatggtgtt
    10321 tttatttcta tcaatgaagc tctttttctt tgcaatcctc acttcaatcc tctataatgt
    10381 cgcatatagt ttagtaaatg agtaaactac taatttactt atttactaaa aataaaagga
    10441 tgaggagtta aaataatgga aataccaaca aatttaaaac ataaaccagt aatcgttgta
    10501 gaagactatg ataaagttga tggaagaaat gcattaaata cggatgcaaa agggctttca
    10561 ctgggccttg ctcagtggaa tgatcgtgga aaagtcgata tctcaggcaa ggtatggcga
    10621 catactggag aaaaatggtc acgacagtcg gaagaattac caatcacacg tattttagat
    10681 ttagcgatct tagctgcaca aggaagttta tacttccaag atgcttatcg ttatgaaaaa
    10741 ttttatgatc cagaaaatcc agtcgtagac atcattggat tacaagggaa ccgaatgacg
    10801 gtagaagtag ataccaaaaa tccaaagatc gatgaagaca tcattttgta ttacgataca
    10861 ctacaaaaag acggagaact gattggggaa cgattccgta ttttaaaacg tcttttagat
    10921 gagctaggat attaatagac agagatgatc aaaaggggaa gagaagcatg gagaatttct
    10981 cactaacaga aaaccgaacc ttttcacggc tagaaaagca aatgatctgg aagaaaccga
    11041 aaagccatcg gacaagtgca gaagaatggc ggatcgcctc cgaaatcaaa gggaattggc
    11101 aagatcctga aatgaaaatc agtaacgtac ttttggaagg agatgccggg tcggggaaga
    11161 cacaattagc caaagcacta tctgctgatc tgcaacttcc ctatacaaaa gtgacctgtt
    11221 ttgcagatat ggacaaatca gatgttttcg gagccttatt acctgtagta gattctaata
    11281 aggaacaaga ccaagaatta ctagaagcta tttaccaaac agatacactt gaagctgtac
    11341 tgacactgat ccagaaccat tacggcattg atcctttttc tgctaaagaa aaattagcag
    11401 agctagttca aaggatcgaa gctgattatc cagatgcaat ccattacagc tattaccctt
    11461 ctgaaatcgt tcgagcgatt gaaaaaggat atttattaga gattcaagag ccgactgtca
    11521 tccgagatgc ctctgttctt gtggcactta actcagcgct cgaaccaaat gggatgttga
    11581 atttaccgac aggaatcatt cgtcgccatc cagattgcgt cattgtgatc actacaaatc
    11641 gaaattacca aggaaatcgc ccattaaatg aatctttaag agatcggatg caacatgcgg
    11701 aaaaaatgga tcttcctgcc ttagaagtca tggcagaacg tgcgatggcg aaaactatga
    11761 tccaaaaaca agaacttctc ttaaagatgg cagagattat ccgtttgttg gatgaaactg
    11821 caaaagctaa cgccatcaaa ggtgtagcag gaatgcgttc ttatttttat tgggcaaata
    11881 cctataagca aggacaagat ttactccagt cgatttatcc aaaagttttg tataaattat
    11941 ccactgattc agatgaattg gcaattcttg aacaagcttt gtctgacagt ggattgctac
    12001 tggaacttca ggacatattg agaaaagaaa aatggggtca tcctcaatct gagcatacaa
    12061 aaggaagaac cattagcgcc gaagaagcaa atgaacgaaa catcactgaa aatgctgaag
    12121 aaattctgcg cttagatgaa aagaaatcgt tgtctgctga agaaccaaat gagggagaag
    12181 agaaacaaga agaaacaata gaaaaaaacg agattcctcc tcaaatgcaa aaagaacgca
    12241 gtagcgatat cgaaggaaaa aatcaaaaag aacaacaaac aggagatgat tctcaagaaa
    12301 tgtctgcttc tgacatggaa gcacatgata aggaaatcaa gaagcaactg aataaagaag
    12361 cgcgacaaat catgaaggaa acgattcatg aaaaagaagg tctcatcgtt caccgaccag
    12421 ctttccaagg aaaaacgccg gaagcacgtg agctacacat gcaagtaatg ccaatagtag
    12481 aatcacttag ccggcaaatt ttagagttac tggataatga acaaacagag acgtaccaaa
    12541 aaggaaaata cgaaggtcag cggttcaatg caagcaaagt agcatatggt gatttgagaa
    12601 attttgacaa aaagaacccg ccacatgagc aaccttcttt agcggtagct gtacggatcg
    12661 atgaatctgg gtcaatgata cgggatgatc ggatcgaagc ggcaaaaaaa gcagcgattg
    12721 ccattgccga attttctaag aaagtcgata tcccattgtt gatttacgga gatacagcag
    12781 accgttcgtc aagagaaaag acttctttgt tttcttataa agaatttgaa gacggatttc
    12841 aatggttaaa tgaaaaatta gtcactatga aaccaaggca aaacaatcgt gatggtgctg
    12901 cacttcgttt agcagcagaa aaattaaaca gacaatccgc aacaaccaaa ctgttgctta
    12961 atattagtga cggtcagcca aaagcgttgc cggattatac aggcaaaaaa gcaaaagaag
    13021 atatccagtc tgttttgaaa gaatatgaac gtcaaggaat cctatttgtc tccgcagcta
    13081 ttggccaaga caaagaagaa atcaagtcga tctacggtga aagtcgtttt gtagatatta
    13141 ctgacttgaa tgaatttccc aagcaaatga ttcaactgat tgcccgctat ctatgataaa
    13201 ataaagctca acgaacgcac ttagcagatt cgttgagctt ttcgtttcta gttaccggta
    13261 acaaggatac ttctaaaata gaatcggtta taatcaagag ggaccgttaa aggagtgaaa
    13321 cgatatgata caagaacaaa aatggtggaa agaagcagta ggctatcaaa tctatcctgc
    13381 tagtttcaaa gatagcaatg gcgatggtca aggagacatc aatgggatcc gtgaaaaact
    13441 taattatttg aaagatcttg gtatcggctt tatctggatc aatccgatct atcaatctcc
    13501 atttgtcgat aatggttatg acgtaagtga ctatcaagat atagaagaga gattcggaac
    13561 gatggaagat tttgatttat tgctaaaaga agcacatgaa ttagggatca aagtgattat
    13621 ggatcttgtc atcaaccata gttcggatca gcatcaatgg tttgaagaat cgaaaaaatc
    13681 taaagaaagc ccgtatcgcg attattacat ttgggtcgac ggggtagatg gcaaagaacc
    13741 gaataattgg acatcgattt tcggcggttc tgcttgggag tattctcatg aaactggaca
    13801 gtattatctg catgtttttg caaaagaaca accagactta aattgggaaa gcgagaaatt
    13861 gaaagaagaa ctattcaata tgatccgatg gtggttggat aaagggatag atggatttcg
    13921 tttagatgcg atttctcatg taaaaaaaga tgaatattca gtaaaagcca cagagaaccc
    13981 tttctcaccc tttcaaaatg tctcaggaat cgaagaacat ttgaccgatt tgaaacatgt
    14041 ttttgaagag tacgatatca tgactgtcgg agaagcaagt ggtgtcactg cagaagaagg
    14101 accacaatgg gtagggaaag acggttattt cgatatgata ttcgaatttg atcatatcca
    14161 tatctggcag caagaaaaag aagggcaatt agatgtcctt aaattgaaac ttgcattaag
    14221 cgcttggcaa acttcattgg atggtatcgg atggaacgct ttatatatgg aaaaccatga
    14281 tgttcctcgt gccgtttcag tgtttggaga tactcgtccc gatttttggg ctatgtctgc
    14341 aaaagcaatc gctatgatgt atttcttttt gcaaggtacg ccatttattt atcaaggaca
    14401 ggaaatcgga atgacgaata tgccctttga atcgatcgat caagttgatg cagtagattc
    14461 gaaaagactt tataaaaggc tgcttgcgga aggtaagaca agagaagaag cattggatat
    14521 cattcgtgaa acaacgagag acaacagcag gacacccatg caatggactt cggaacaata
    14581 tgctggtttt tctacacatg aaccttggct gatcactaat ccaaatacca aaacgatcaa
    14641 tgtagaacaa caagaatatg aaccagagtc ggtcctgcaa ttctataaga acatgatccg
    14701 cattagacaa accaataaag gtctgatata cggttcttat aaagaatatc ttcatgaaca
    14761 tccacaactt tatgtctatg aaagatattt agaagatgaa caatatctta ttatggtaaa
    14821 cttaacagaa tcacttgctg actatgaact tccaaaagaa gcagatcagt cctggacatt
    14881 actcttgtca aattcctcta gcggagaatt tgaagctaaa ggcatactag caccttatga
    14941 agctcggtta tataaaacaa acaagtaaaa cttcctcgtt aaagttcatt agttggactt
    15001 tgcgaggaag tttttcagat tgctctgtca attactcatt tcaataattc gctctaggac
    15061 caccgatata tttaggacga gagcgaaggg ctgtcacgtg agcttgtcca agggttttca
    15121 aacgaggtct gacaggtact tggacatgtt tgatatgcat accgattgat gtgtcaccga
    15181 tatcgatacc ggcttgtcca actacgtgtt ccacttcgac aggatcattg aactggtcaa
    15241 aagcagcaac agaacaagca cctccagcat gtagtgcagg aacaacagag acaatttcga
    15301 aattttttgc ttctgccact gttcgttcta ctactaatgc tcggttcaaa tgttcacaac
    15361 cttggacagc tagcgcaatt tctttaggat ccaacaactc ttttaacgta cggatgatcc
    15421 attgaccaac ttccgcactg gaattttttc caatatgtcc tccaacgact tcgcttgtgc
    15481 tgcatcctaa aacaaaaaga tcaccttttt tcaagtgtgc ctcagccaat atgtcattaa
    15541 ctatttcagt caattggtct tttaattctt tttctgacaa cttcattttc taaaaccttc
    15601 tttttcaatt aatattgctt ccgtgcttga atacgttgaa gacttgcagt tataggaaag
    15661 gcaagtccca ttccaatcag attttggaag atgtttcctg gtatagaagc cactccagcc
    15721 ggccaatcat ataaaatcca accggcgata ccatagccga tgatcataac taagcttcct
    15781 aataaaagcc ctaatcccca catacgagta gaagatttgc tgaaagaacc agcaacatat
    15841 ccttgtaacc cgtggatcac taatgaaaaa actgcccact gcggatatcc tgaaatcaaa
    15901 tcgatcaata gaccactgga agcaccaaca actaatccac ccgttggacc ataaagaaaa
    15961 gcagcggtat agatcccggc ctcgcataat gtcacaaatc cattcgtagc cggtacaggg
    16021 atcaaaattg ttaatgaaag ggcaactgtc aacgcaatca gcatagcata gatcgttaat
    16081 tgcttagttt tcatttctca ttcctccttt ttgaataccg ccagaccaga cacttccggt
    16141 atgatcgttc atcagcacgc cattagaaat acaatgggca acaaattttt tactttctgc
    16201 tactgctttg gtaaaatctg cccctaacac aagattcgct gttattgcag aagaaaaggc
    16261 acaaccggcc ccgtttactg ttgtagtgtc gattttttta tgagaaaata cctcataatg
    16321 atcaattgaa tagagaagat ccacagcttc atctccagcc atccttgtgc cgcctttgat
    16381 cacaatgttt tttgttcctg aagcagtgag ttctttagcg agatggatca tttctgttaa
    16441 cgaagaggga agagcttgac cagaaagtaa agcagcttct gccaaattag gcgtaatcat
    16501 cgtaacaagt ggaaaaagtt cttcgatgag ctgcgaaata tacaattgat tgatcacagc
    16561 ttcagtttct ttgaatgcca aaacagggtc taaaatgatt gggatatttt gttttgtttt
    16621 tatgaagtct cgaacaacca tgattgcttc acttgaattc aataacccaa ttttgatacc
    16681 atctaatgaa gtcattttat caatcgaaac taactgtcgt ttaagcaatt ccaaagatat
    16741 agattccagc ataaatcgct cattaatatc tagtgcccct atagaagtca aggcgcttag
    16801 tccgaataga ccaaaattct caaaagtttt taaatctgtt tggattcctc caccagcaaa
    16861 tggatcagaa ccgccaattg tcaagattct tttcataaca ttttcctcca tgaataagag
    16921 tataacgaaa ttttcaaaaa agaaaaacag ccaattggca tatttttatc tcatccaatt
    16981 taaaaaaaca aaaagaaaag aaagaccaaa atggatcttt ctttccttaa atagttagga
    17041 cttttgtgat tttaattctt tctcgatttg aagccgttgc actttcattg tagctgttcg
    17101 cgggattgca tcaaaatcac ggatgatagg gacgttcaag cgaggaagtt catggacttg
    17161 ctcccaccat cgattccaat ccatttcttt atcaggagct aaagcaataa tcggttgtgg
    17221 agaatgattt ttatccctta caatcactac ttctgccaaa aaatctaacg aatctaataa
    17281 aaagtcttca atagctaatg tactattgat cgtttcgatc aaatccactt gccgatcttt
    17341 caaaaatagg tggccatgct tatccataaa accgtaatct ccgctatccc accaatcgcc
    17401 ataaacattt tcctggaacc gtgcatcttc cttgtaataa gtcaaagccc gtcctttaga
    17461 cagtaattgt atatgaccat ctgtgttttc aggtaggaca ttcccaactg aatctgtaat
    17521 ccgagcgctt gtcatgtctt ccagtccaac gcccatatct cttgcatcac ttgttttcaa
    17581 actctctagt gtatgagcct taagaatcat tggtccgcat tcactttggc catatacttg
    17641 aagaaatacc gcatcctgtg ccatcgacgt tcgcaaaaat gtcttcattg tctggttgtt
    17701 tatagcatca aaagtggagt gatagtatcg gatacccgaa aaagcttctg gatgctcctt
    17761 agcagtaaaa cgccactgaa caaagttatt cggatgggtc tctagtgcaa ttggccggtg
    17821 agcttccaac atatgaacaa cttttgaact ttgtgcgttt gctaaaggca tcataggaaa
    17881 tcccatggac atcaaagaag agataccaat attgaatctg gaatgcactg gagagatgtg
    17941 aaaagcgatt aatttctttt ccgaaatttt tgtgaaaact gttttttgcc atttcgtccg
    18001 ccatcccata gaatgagcag aatgacaaat aagtttaggg ataccagttg taccagaagt
    18061 atgggtcata taagaaattt catttttgtc taaaaatgat tgagaaacag acaaagcagg
    18121 ttgttccatt aaataaagaa taggaa
//
LOCUS       DAYYCW010000058        17567 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000058 DAYYCW010000000
VERSION     DAYYCW010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.058"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..979
                     /locus_tag="ACHAYA_001762"
     CDS             14..979
                     /locus_tag="ACHAYA_001762"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8605559.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(1078..2250)
                     /locus_tag="ACHAYA_001763"
     CDS             complement(1078..2250)
                     /locus_tag="ACHAYA_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8605560.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(2237..3382)
                     /locus_tag="ACHAYA_001764"
     CDS             complement(2237..3382)
                     /locus_tag="ACHAYA_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8605561.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(3396..4328)
                     /locus_tag="ACHAYA_001765"
     CDS             complement(3396..4328)
                     /locus_tag="ACHAYA_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8605562.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            4445..5524
                     /locus_tag="ACHAYA_001766"
     CDS             4445..5524
                     /locus_tag="ACHAYA_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8605563.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            5521..6139
                     /locus_tag="ACHAYA_001767"
                     /pseudo
     CDS             5521..6139
                     /locus_tag="ACHAYA_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(6249..6476)
                     /locus_tag="ACHAYA_001768"
     CDS             complement(6249..6476)
                     /locus_tag="ACHAYA_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8605564.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(6579..9167)
                     /locus_tag="ACHAYA_001769"
     CDS             complement(6579..9167)
                     /locus_tag="ACHAYA_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM8605565.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(9187..9846)
                     /locus_tag="ACHAYA_001770"
     CDS             complement(9187..9846)
                     /locus_tag="ACHAYA_001770"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8605566.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLLNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(9895..10398)
                     /gene="trmL"
                     /locus_tag="ACHAYA_001771"
     CDS             complement(9895..10398)
                     /gene="trmL"
                     /locus_tag="ACHAYA_001771"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM8605567.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(10619..11485)
                     /locus_tag="ACHAYA_001772"
     CDS             complement(10619..11485)
                     /locus_tag="ACHAYA_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8605568.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            11594..12235
                     /locus_tag="ACHAYA_001773"
     CDS             11594..12235
                     /locus_tag="ACHAYA_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM8605569.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(12297..13658)
                     /gene="mgtE"
                     /locus_tag="ACHAYA_001774"
     CDS             complement(12297..13658)
                     /gene="mgtE"
                     /locus_tag="ACHAYA_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM8605570.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(13676..14575)
                     /locus_tag="ACHAYA_001775"
     CDS             complement(13676..14575)
                     /locus_tag="ACHAYA_001775"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8605571.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(14577..15374)
                     /locus_tag="ACHAYA_001776"
     CDS             complement(14577..15374)
                     /locus_tag="ACHAYA_001776"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM8605572.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(15352..16056)
                     /locus_tag="ACHAYA_001777"
     CDS             complement(15352..16056)
                     /locus_tag="ACHAYA_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM8605573.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            16187..16762
                     /locus_tag="ACHAYA_001778"
     CDS             16187..16762
                     /locus_tag="ACHAYA_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM8605574.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            16880..17557
                     /locus_tag="ACHAYA_001779"
     CDS             16880..17557
                     /locus_tag="ACHAYA_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM8605575.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         5191 a         3783 c         3130 g         5463 t
ORIGIN      
        1 gcaggaggaa atcatgaaat tccattatca tatattgatc aatccatcgg caggcagcgg
       61 aaatggctat aaagtagcag aaagaatttt accggtatta aaaaataagc atattgacta
      121 tacacttcat tatagtgaat acaaaggaca cgatgctgag attgctgaaa ctttggcaaa
      181 agaaacattg attccttgga tagaagaaga acagaaaaca atcgatactt tccccctttt
      241 gatcatcgta ggaggtgacg gcaccttaca tcaggtactt gatactttct atcagatgga
      301 agtagaattc ccagttgctt atattccagc aggttctgga aatgattttg ctcgaggggc
      361 aggccttcca aaaaatccca aaaaaggact tcagctgatt ttagcagctc aatcaccaga
      421 aaaagtccac ataatggctt atgaagaaaa aataagtgaa aaaaaaggaa tagctgttaa
      481 taatttcggt ataggattag atgcagctat cgtccatgcg acaaatcatt cttccacaaa
      541 aaagcgattg aacaaataca acttaggatc attttcgtat ttattctcta ttctccgtgc
      601 tttattcact caaaaaggat ttccaattct ggtagatgca ggaggaaaac aattaagttt
      661 ttcaaacgca tttttatgta cagcaacgaa gcatcctttt tttggcggag gaatcgcgat
      721 tgttccaact gctgatgcgt ctaaaccagt cattgatttt gtcgtggtcg aacgaatcaa
      781 tttctttaaa atcttgtggc tcattctttt acttatacag aaaaaacaat tgaaatcgaa
      841 atatttccat cactacacaa caaacagatt acgtatcgtt tcaacaacac cgcaacatgg
      901 tcaagaagat ggagaagaaa tggaaaaacg accttttgat atcacaataa ccacaactaa
      961 acagttgttt tggtgttaat aaaagaggtt ttgtcagaag ctacatctgg aacaccgttt
     1021 attcggaaaa aaggactgtg acacgacttt tgtcacagtc ctttttttac tatgcagtta
     1081 attctttgcc caattgggtt gccatcgata gtttctttct ttacccaaaa taaattggat
     1141 cattgaaaac accgcagcaa aagcaactaa gacacaacca ttgaaccaaa aagattctgc
     1201 aaatgtttga ttgtaactga gtgtttcact gatcaagtta ttattttgaa caaaccaata
     1261 tgttggtgtg aatgccgcta ttttgctaac catttctggc aagtattctg ctggtacaaa
     1321 caccccaccg ataaagcaac tgcctgtgat aaaaatatct ttgatcccac cgctggtttc
     1381 tgagtttttt accaatgaag tgatacaagc cgaaaagcta atgatcggca ataaaaacaa
     1441 gaaagtattt aaaatatgat accataccgt tatatcccac gaggtgtgga tcactaacag
     1501 aaccattaga agtaagaagc cactgaacaa cagcagcgag taactaatca aactaaaggt
     1561 tactttgcga ttcaagtttc gtttggtaat gggcgcactc tggtttcgtt tactgatctc
     1621 tttgcgatta aacgctagat tgatgacgct gaaaccgcta aaaatggata gaaacaaccc
     1681 ataagacaag aaattaaagg ttgtgcccgt caacagcttt ttcatccgct gactatatcc
     1741 cgcacttagc tcaacttctc cagtaaccga gagtgccatt tgtgtctgtt ccatagctgc
     1801 atcgattgtt ttatcttctt cttgaaacag aatcagtgtt tgcaaaaagg tgttcacttg
     1861 ctgtgtaaca actgttttag aaaaggcatc tgggcgactc tgtatcttga tcgtcgccat
     1921 ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga tgtaagataa tgtttacacg
     1981 attaaaataa agagcatcat cgatttcttt ttgtgaggtt ccatccaatg taacgactgt
     2041 ttccttgtca ttcaaatatt tacgaaggcc atcgactaca ggcgactctt cttcactaat
     2101 caaggcgatt ttcgcatctt gcagagaggt attttcaaca tcaatatttt gcgcaaaaaa
     2161 gaatgtggca acaacagtga taattacacc tatcagtaaa ttcgatttat ttgctttaag
     2221 caatgtccaa atcgttttat aaagcgtcat aacgcacccc tctttccatc caagcatttc
     2281 ctaacgcaaa aaggaaaata aatccgatca accatagaag attggtataa aaactagcca
     2341 aatcagtgta gaaataaagt ttatacaaac tttcgctgat caagttgacg agatttaatt
     2401 gaccaacaat cggaatatga agatcaatcc aatacttcac ttgttccgac cccatcatac
     2461 cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat gctatccttt tgcaccagat
     2521 tcatttttgg tatcaaatta ccaaccaatg tacctaacaa aatcgcacac aacgacccca
     2581 gagcggtaac aatcatcaac cattgccaac gttcaccaaa gtctacttga tagataaaac
     2641 gaaagacagc taacataata taaatctctg tcaaaacaat cgtaaagctt gctgccaaat
     2701 tagcgattga aactaacgat tgctttctag gaatcaagga caagcgaata ccatttgacg
     2761 attgatttgc ttgctgatcc tgtacattgc ggacccccca catcgtcccg tagagaatcg
     2821 ccattccaat cagagtgaag aaataaaaac ttttgaaact catattacga cctgcatgaa
     2881 cttcttgaaa agatacttct tgttcaaaaa cagtcatgat gggcgcgaga tcaacgttct
     2941 ccacttctgc cactttactc gaaattatac caatacgctg taaaaattga tccatgacgt
     3001 ttttcatcaa cgtctgactc attcctgctt gacccacatg gagcgaaaca gatgaaccat
     3061 caatgagata aaaaccagcc aaatcatctg catttagctt ttcattcgct tctacttccg
     3121 atagttcctg tacttcaaat aatggtttat cattgacttc gacttgttct aagactgtgg
     3181 taaaattttc cgaaagcgct gtttgatccg ctttgattac aattccagta ttaatcgttt
     3241 caagattgcc accttgatca atctcgccaa aggccgccat aaacatcaat cctaaaagaa
     3301 ttggaaaggc caatgtccaa aacaacatgg atttatttcg cagcagtatt tttgtgcgat
     3361 atttataaaa atgccaaaac attatttccc ctcctctagt ctctcaaatc cttgccagta
     3421 atctctaaaa agacatcatt caaggacgct tctttggttg ctatttttcc gtaactgatc
     3481 ttttcttctt gtaagattgt aagaatcggc aacaatactg tatttagttc agtagttgtt
     3541 acttggagct gacctttttt ataagcgatt ccatcagaag ctattgttcg ctgaatcgct
     3601 gacaacacat tttctgggag taatgggatg tcgatcgtca aacgatccgc cgtccgtacc
     3661 atatctttta gagattcttt ggttccttta gcaatcacct gtccttgatc catgatcacg
     3721 atctggtcgc aaagaatttc tacttcttcc atgtaatgtg ttgtatagac aatggttgct
     3781 ccttgtcgat tcagctcctt gatactttct aaaattttat tccgactttg ggggtctaca
     3841 gctactgtcg gctcatctaa aaaaattaac cgcggcttgt gggcaatccc acatgcgatg
     3901 ttcaaccgac gtttcaagcc accactaagt tctcctggac gaaacgaaac atatttttct
     3961 aatcccacta attgaatcac ctcttcaatc aaggctttcc ttttctcttt ttcggaaaca
     4021 tataaaccgc aaaaataatc gatattttct tgcacactta gttcgtcaaa gaccgcaacg
     4081 tcttgaggaa cgaccccaat cattgccttg acagcataat tttcaggagt cattgttgtc
     4141 ccgaacaatt taatggttcc tttgtcataa tttaacaaag aaagcataca gttgatggca
     4201 gtcgatttcc cactgccgtt tggacctaac agacctaaga cttctccttc ttgtacttgc
     4261 agatcaaaat gattcaatgc tacaaaatct ccatagcgtt tgactaattt atcaatttcg
     4321 ataatcatca tgaaaaactc cttccaaatt tcttgttgat ctgactataa taaagtgcaa
     4381 gaaaagaaac tagtgacgat tggctaaacc acacatgaca tttgtcacaa tgaaggagtc
     4441 tgctatgcat aaatctcggc tactcaacga aactagcttt ctactggtta tctcattgct
     4501 tttgctggta agtcaaggat tcgttgctat gcaagttatc tatttattga tcgccgtttt
     4561 attcactggt gtcagttatt tactgcctac aaagccaaat tggattttcc aagttattct
     4621 atttctttta tgtgcctttt tacctgatct tgtcctgttt ctgccagcga cgatgcgagt
     4681 gatcgcccct gaagaaagcc accttgatta cttgtttttg ttaggttttt tgttgatttc
     4741 gacggttggt aatcatcccc ccatcaaggt cgcctttgtc gtaattttgc tgtttttttc
     4801 cttttactta cgaaaaaaag atcaacgaac gaccgatctt gagaaaaagt acattgtctc
     4861 caaagatact cattgggaaa aacaacaaga gttatcggaa caaaataaag cattggccct
     4921 gtcacaagaa acacaattag ccttgcaaat tgtggaagaa cggaaccgaa ttgcccgaga
     4981 catccatgac aatgtcggtc atctgctgtc aagtgcgatt ttacaaattg gtgcgcttga
     5041 aatgatcaat caagaaccaa aactaaaaga accattagaa aatttaagtg ccaccgttca
     5101 cactgggatg gatcggattc gtcaaagtgt ccatgacctc catgagacat cgatctcttt
     5161 tcaacactcc ttagagtttc tgatcgctga ttttccttct tctgttacga ttgaaggaaa
     5221 cttgtttgaa ggattgaatg aaacgcagca aaactgcttc ttaatggtca tcaaagaagc
     5281 cttaaccaac agtatcaaac atagtcatgc caaaaccgtt gttttacatt ttcgaacgtt
     5341 gccggccttt tatcgcttgc aaatcacgaa tgatggcacc agcccacaaa aaaattctgc
     5401 tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa gaacaaaagg gacaagtcca
     5461 tattttccaa gaaaaccagc aatttcagtt aacgatcatt ttaccaaggg agggacacca
     5521 atgataacag tagtcattat tgatgatgat ccatttgtga cgaaatcgtt acaaaccatt
     5581 ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc attgtgccaa ggatgcgcga
     5641 aacccatcgt cccgatgttt tgctgacgga catccgcatg cctaaacaaa cgggcatcga
     5701 tgctgccaaa gaaattttgg ctaaatttcc caaagccatc cttttgcttt tgacgacatt
     5761 caaagatgaa gaatacatcc gcgaagcatt ttccatcggt gtcaaagggt atttaatcaa
     5821 acagaacttg caagcgatta ttccttctgt caaatccgct tataatggac aggccgtatt
     5881 tggtaatgaa atcatcgaga ctttcactca aatgatgaag aataaaccga cgattgatag
     5941 tcatgcttat gcttctttta gtgaacgtga gcttgcaatc atcaaagaag tagctgccgg
     6001 gaaaaacaat aaagaaattg cagatgcctt gtacttgagt gacggcactg ttcgcaatta
     6061 catcagccaa ttactagaaa agctagattt acgggatcgc acgcaattgg ctatctattt
     6121 ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa cctgaatgat gctccttcgc
     6181 atcattcagg tttttttcac atcttctatt caattgtccc gcaaaccatt tcctcacgat
     6241 tgaaagaatc aaggattcgc agggctgcca ggattcgttg gattgttcgg cgtgttaggc
     6301 ggaaaaatag gcgccttgtc taacgttttt tctagttttg attcttcacg atcgtctcgt
     6361 tctacatcag gactttcatt caatttgaat tctttttcca tctcatctaa ctttttaaag
     6421 tcattgccag attcttttgc taatttcgct aatttatctt cggtttcttt agccataaag
     6481 taacgccccc cttttgattt cagcttagca tatcacactg aaaaaacaaa agaaaacgct
     6541 aaccaacgat catttttagc tatttctcat ctttaaaatc aagcattttt tgccggtata
     6601 aaatcgtagg gagaactgtt ttccatacca atcatcggat ctactaactg gttatcgatc
     6661 acttctgctg taagaacgaa atctttagaa acagcaggtt ctacctcttc tttcttttct
     6721 tctccaacaa atgaagaaaa cagatctggt tccactgttt ccattacttg gtttttcgtt
     6781 tcattcgtcc cactgtcatc ttctacaggt agcttttctt ttacaacccg tggctctttt
     6841 gtatagttcc catcttcaaa tggatcattt ccgaccaaag aatcttcata agctgctagt
     6901 ttggtccgga gagtgatatt cattttatca ttatccatga tccttgattt caacgtagtc
     6961 atccggcttg ctgattcatt cgctacacaa gtgacatagg cttgctcttc ccctaacaaa
     7021 atcaacagtt ccttactgcg agtcactgcc gtgtagagca aattacgctg aagcatccgc
     7081 tgatattgat ggaccatggg caaaataacc atcttgaatt cactgccttg tgatttatgg
     7141 attgagcaac aataagaaag agtgatttta ttccattcgt ttcttttata agtgacctca
     7201 tttgcatcaa actggataac taattcatcc actttatctt cagaatcttt tgccaagatg
     7261 atacccgtga tttctcccat atctccattg aatacattca attcgggagt attgactaat
     7321 tggaggactt tgtctcctat ccgataaacg gtgtcgttcc aatgtacttc cttttttcca
     7381 ggtttccctg gattgaatat ctcctgcatc attttattta acgcatcgat ccctgcaggt
     7441 ccccgataca ttggtgctaa tacttggata tcttgtgcac ggaatccctt atttttcgct
     7501 ttttccacaa ttttagagat caagggttca atttgataag catcacttcg gaaataggaa
     7561 cgatcttttt gattctttgt gaaatctttt ggcagacgtc cttctttgat ctcatgggca
     7621 agaggaataa tactagaacc atcaccttgc cgataaatct gattgagttc cattttggga
     7681 atttgttcga tttccaaaag atcatggagt acttgaccag gaccgacaga gggaagctga
     7741 tctttgtctc ctactaagat aacttgcata ttcgtcggaa tcgctttgaa taatgtgtta
     7801 gccagccatg tatcgaccat agacatctca tcaacaatca aaagtccgcc ttctaattct
     7861 ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc ccagaagacg atggatcgta
     7921 ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg ctcggccagt tggtgctgca
     7981 agtagaatag gaaaaatttc ttgggtatat tttgaaggat ccaattccag tccgttcaac
     8041 tctgcaaaaa gattgacgat tccgttgatc acagtagttt tccccgtacc tggtcctcct
     8101 gttaaaataa atagagggga acggattgct tcagcgatag cttgttcttg cgaaggtcca
     8161 tactgtatgc cgaatatttt ttcgatacgc cgaatattct tctgcacttc tttttcagga
     8221 taatggattt cttttttacg actgagaagg cgctggatcg aattaccgat cccccattca
     8281 gaaaagtaca aactgttttc gtaaagatta gtctcttctt gttgtatctt tccttcttca
     8341 acaagttgga taattacagt agcaacctga tcaggctgga tttcaattgg gcgacttgtt
     8401 tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg gtacgtatgt atctcctgtt
     8461 tgaactgcat gctgaaaaat ctgatgcaag atagctgctc gaattcttct cggtgaatct
     8521 gctgcaatac cgatttgttc ggctagatta tctgcacgtt tgaacccgat tccttctatg
     8581 tcttcgacta gctggtacgg attttcttcg atgatatcca aagactcgtt cttataggcc
     8641 tggtagatac tgaaagctaa ctgactaccg aacccgtaac gattcagtcc catgatcact
     8701 tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca tttctctttt tggcttcgtc
     8761 aaaccggaga tttgttttaa taccgaagga tcagcaatga ttttttcgat agcatcttct
     8821 cccagcaatt ctacaatttt ttctgctgtt ttcttcccga ttcctgggaa tttttcgctg
     8881 gaaagatacg ctacgatccc atttgcagtt gttggctttt cttgctcata tctttcgagt
     8941 ttaagctgcc ttccgtattt aggatgatcc actaattctc cataaaaacg ataagtctca
     9001 ttttcttgaa tatcgccgaa acttccggtg actacaatct ctttttcacg gtaatcagct
     9061 gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat tactaggatt ttgaaaaaaa
     9121 atcgcggcaa ctgtaccaac gaaataaggc atttcttctg gctgcatcgg aagccgcctc
     9181 cttcttctat agtaaaggtt ctggtgctgt ttcagttcct aaaaaatctg tttggttcca
     9241 tactttcagt tcatatggca ataatctgca aggctcttca gtctctaaaa tcgtcaaact
     9301 actatttaac aatccaccca tttcacgcaa ctcacttagc tcttttcctg tcatccattg
     9361 aatggctgca gttaaagaag ctccatgtcc tacgaataaa agtgggccat ctttatgtcg
     9421 ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt gccgattcaa tcgtttctcc
     9481 gtgaaaagga gaaggatcat acagatctaa atgattacgc aaattcggca gattatccgg
     9541 atatttctgg tacatttcat cgatcgactg accttctaaa gtacccagcc ctagttctct
     9601 tagcttatct gtataaaaaa tctctaccgg acgagcaagc tctttgttga taccttctgc
     9661 tgtatcacgt gctcgctttg ctgtactgga ataaattgct ttaaaaggaa catattttac
     9721 ctttttccca aatgctttga tttcttcgta actttgtggc aaaagcggag aatcgccatt
     9781 gctcccttga aaacgtcttt cctgattcca ttgtgtcttc ccatgtcggg taaaatatag
     9841 ttccatacgc agcaaactcc tgtctttctt ttattttact ctataataaa ctgtctaatc
     9901 cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta gggaatcctt gctggcgaag
     9961 tgcttcatac actatcattg cagctgtatt cgataaattc aatgagcgga catgctcatc
    10021 attcataggg atgcgtaaac acttttcttc gttttcacgc ataaatgttt ctggcaaacc
    10081 ggttgtttct tttccaaata aaaagaagtg ctcttctcca tcagcaaaat tttcgtcact
    10141 atatacttta tgggcaaatt ttgagatcaa atgtagttta cgattaccta aaaatgttaa
    10201 aaaagcttct aaattactgt ggtaagtaat atttacatca ttccaataat ccaaccctgc
    10261 ccgttttaga tgtttgtcat cagttgaaaa acctagtggc tcgattaaat gaagcggagt
    10321 attcgtggct gcacacgtac gggcaatatt tccagtgttt gcagggatta atggttcaaa
    10381 taaaacgata tgattcatca ggatcattcc tctcatattt atgttcgttt ctattttctg
    10441 acaatttgat ttttgcataa aaaaaaacgt atcaactcgg tgtcagtcac tgcgagtcgt
    10501 tacgttagtg aagctcttct cactaacttt tatcagaaca ggaacctgat aaaaaccaat
    10561 gaaaaaacaa cttgttgcta atataacacc ctcaactttt ttttgcaacc tctactcatt
    10621 aaaaactgct agaaaaacgt tttctcttag ttccttttaa taattctatg tcaattgtaa
    10681 tcttcttcaa aggattctta tataagtctg ctttgattcg ctcttctacc tgccattcta
    10741 aaggtgacat ggccaaaaga tctttttgat tttcctctgg tatctcaaat tcataggtta
    10801 cacgctgacg ttcagttaat ttcattgctt cagcaaactt ggctactaca cgttcattgg
    10861 aataactctg tttttcaggt tgattcggat aaaaagcctg acgtagttcc tttaaataat
    10921 tttcttgtgg caccaccttc aacacttctc catcttctga gagtacacgt tggaattctt
    10981 ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa tgtttgatca gcaaacggca
    11041 aattggtcaa atcagctaca caccaaaatg cttcggcaga ttgttctgta gctgcgtaga
    11101 ctccttcttt tgaaatatca aatccaacag cattttgaat ctttttcaat tttgaaagtt
    11161 cattcaagaa gctaccttct ccgcagccaa catctaatag cgagacttct tcaggtaaat
    11221 gctgcgcaat agtctcaata acgggctgat acatgccaga ttggatcatt ctacgccttg
    11281 gaacgaacat tccttgatcg tattctgttt taaccgcatg ggataaaaaa taaagtgttc
    11341 cttttttgga aaggtcaaaa cggtgattat ttttgcatat caagccatta tcagactttt
    11401 ttacttcttc ttggcaaacc ggacagcgga acagctgatg attttctgcc aaaaactggc
    11461 gtcctcggtc aatttttttt aacatagttc cccctcattt ctgttttatc ttaccgttag
    11521 atgctcactt tggcaactgc taaaaatatg atacaatgcc tagaagaaag tgaaagataa
    11581 aggagaagat tagatgatca aagaattttg cgctgaaaac tacacatcta ttcctctagc
    11641 aattgctaga ggagccgatc ggatcgaatt gtgtgacaac ttagcggtcg gtggcacaac
    11701 cccgagtact ggtgtgatcg aagaaacgtt aagttatgct ggggaaaaag atatccccgt
    11761 aatgacgatt attcgtccta gagggggcga cttcgtctat aacgatatcg aattgaaaat
    11821 catgcatact gatttgattg aagcaaagaa attagggacg gatggcgtag tatttggttg
    11881 tcttactcct tcaggctggc tagacgaaga ggccttagaa ctattgatcg aaacagcaga
    11941 agggttgcag atcaccttcc atatggcttt tgacagtatt cctgaagatc ggcaattcga
    12001 agcaatcgat tggctggtag aacatggtgt tgaccgtatc ttgactcatg gcggtcctgc
    12061 tgacaaaact attgtagaaa atttcccacg gcttaaagaa ttgattgcct atacagatgg
    12121 ccgcttaatc attttgcctg gtggtggtgt cactgcagag aatgcagcac atgtagctaa
    12181 agaattgaac gtaagcgaag tacacggaac caaaatcgtg gatcttcagt tataatctta
    12241 taaacaaaaa aacatttgca acaggcataa tgcttattgc aaatgttttt gtttttttac
    12301 ataaaatagc ccataaataa agaagcaata ttgaaataaa tcagtacact cgtcaaatcg
    12361 ctcaaagtcg tgataaaggg accgcttgct actgctggat caaatcctag acgatccatt
    12421 agcatcggga ttaaactacc tgcaagattc gccactgtga ttgcaaatag catagccatc
    12481 cctatgacaa accccaagat aaaattatgt ttccaaaaac ctacgattag aaaaatcgtg
    12541 atccccgtaa cagctcctgt gacaacgccg gtcaagactt cagccaaaac aagtcgaaca
    12601 aatccatgat ctttctcatc agaaatcgca agtcgacgaa cagccacagc tagcgactgg
    12661 gttccagcat taccggcagt tcctgtaatc agcgaaatga agactgctaa aatactcgct
    12721 tcacttacca agtcttcata gtgactgatt aaagaagcgg tcgacatacc taaaaacaac
    12781 agagtgatca gccaaggcaa acgtcgagac gctgccttta ctggattttc attaatttct
    12841 tccacattga ccccggcaag tcctgaataa tcgctagcag cttcatcatc aataacgtcg
    12901 atgatatcat cgactgtgac gattcctaac aaatgatcat cataatcggt aacaggaaga
    12961 gctaagaagt cataatctct aaatgtttgt gccacatctt cttggtcgtc gcctacatga
    13021 actgaaagca ctcgttcgct catcagatca gaaatcattg cgtcgtcatc attcacgatt
    13081 aggtcgcgca gagaaataac ccctactagc tgaccttctt gattaacgac atagatgtaa
    13141 tagattgttt cagctacatc tgcttcgttc ttcagcacgt acatcgcaga gcgaacagtt
    13201 tgattggcta cgatcgaaac aaattcagtt gtcatgatcg cgccagccgt ttcatcctcg
    13261 taatggagaa gttccttgat ttcactggca tcgtctgccg acatcaaact taaatattta
    13321 gcaatttgct ttttatccag cgtattaagc aagtcaacag cattatctgt atacatctca
    13381 gaaagcatat cagctgcata gctaggacgc atttcatcta ggtattcttt catatgttcg
    13441 ttgtcttctt caatcacatc gaacatatcc gctaattctt taggcgacag ataggaatag
    13501 atcagctgcc tgttctcttc tgtaagagat tggtagaatt gcccctgctc atagatatgc
    13561 atctctaaaa aactgtttct gaatgcctga atattatttt cccttaaatc ttgagtcaac
    13621 tgggcaaatc tttcctctaa ttcttgattt tcatccaaac caatctcacc tcgccttatt
    13681 cttcaaaccg tctcacaact gcctccatgt ctgcggctaa aggctgcttg aactccagca
    13741 actcttctgt aaatggatgg taaaaacgca gatcataaca atgaagggct tgtcttttta
    13801 ttcccagatc cattcgcccg ccatacatat catcaccgag cagggcacag ccaattgcgg
    13861 aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag ctggatatca actaacgcga
    13921 tttctgttag tcgcttctct atccagtact cagtctgtgc cgaacgtcct tcatcagaga
    13981 ccattcgctt caatagcgaa gaataatctc gggcgatcgg caaatcgata tgtccatgtg
    14041 ccgataaatc tgaaagcctt ccactgacta aggcctgata ttttttcaca aacttctttt
    14101 cacgaagttg catatctaat tttgcgtgcg caaatccatg ctttgcaaaa agcatcaagc
    14161 cagatgtatc ccgatctagt cgcgtcacca catggattac ttggttttcg taaccttgtt
    14221 ttttataata agcctttaca cgattagcca ttgtcccatt tggatggtat tgggcaggaa
    14281 tagacgaaac gcctgctggt ttatttacta ccagaacgtg ttcatcttca tacacgatat
    14341 cgatcggtgt ttcatccaaa agtacggttt catgttctcc ttcagcagga atcacaatgg
    14401 ttaccttatc atccttagct agagaaaaca agacattttc tacttgatca ttaactttga
    14461 tttgaccgcc ttgaaatttt attttcgcta acagaccctt tgagatccct ttttctttta
    14521 aaaaatattt tacttgctga gaatgatctt tctcataatc ccatgtaaac tgcatatcaa
    14581 tcctcactaa taaaggcatc tttcacacga tgccaaaaat gcatatgtcg ataagaagca
    14641 aaatggatcc gttcttctgc tattcgataa taaattgagc ggatatttgc ttttgcgaca
    14701 ttcaattgat caaccgttac aagataatct tctgtatttt ccaattttat ttctagccat
    14761 tcatcatggg caatcacgat cggagaaccc aaagtacgaa acactcgatt attcagtgaa
    14821 gcaatctctg ccaactgaaa ggcattgata ctaggatgga tcacagcacc gccgacactt
    14881 ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca atccatctcc tctaaaactt
    14941 tcaaacagct cgtttttgat gtatacatcg gctaccatcg tacgattggc ccgtttaatc
    15001 gttgattcat tcagtgctaa aaaatgctgg tccggtgttt catccaaata actgatccga
    15061 acatccagca agggataact aacactttgt tcccggttcg ttcgtaagct gtctactagt
    15121 tcttccagct cgtaatcccg ccaatccgtg taaaacccta aatgacctgt atgcacgccc
    15181 aagaaacgca catcattcag acaatgactg tagtgatgga acgccgacaa taatgtgccg
    15241 tctccgccga ctgagatgac caattctggc tcatgatcat ctcgttgaat ccctgcttgt
    15301 tctaaaagtg cgttcaaacg ttctgtcact tctatagtct ttgcttcttt attatgaacg
    15361 atcgctactt tcatcccctg cctcctctct ttcgctttct ttcttcaaaa gcgttcgata
    15421 gtagacatca tctgattttc ctcttccatg ggagaataac cgctgtgctt cttggatttc
    15481 ttcacggata ctggacatct cttcatccaa ctgataggca gcttctgccg cacgctgaag
    15541 tcgttcactc atttcttctg gaaaaacgcc ttgatattta taattcagcg aatgctcaat
    15601 cgttgcccag aaattcatcg caagagtccg aatctggatt tccgccaaaa ttttcttctc
    15661 accgctgatc agctgtacag gatactcgat caccagatga taagaacggt agccacttgc
    15721 ttttttattt gtgatgtaat ctcgttcttg gagaactttc atgtcttttc ttttacggat
    15781 gatctcaaca acttgatgga tatcttctac aaactgacac atgatcctta acccggcaat
    15841 atcagacatt tcttcttcta aacgtcccat tggaatatgc cgtaattgtg attttgttac
    15901 aatactatcg actggcttga ctctcccagt gacaaattca atcgggacat gtcgattttg
    15961 ttcacggaac tgtttgcgga tcccccgcag tttcactttc aattctgata cagtttgttc
    16021 atatggtgct aaaaatgctt cccaatctcg ttccatagct gatacctcta cttcattaac
    16081 attcattgtt tattctacca tatatgcaaa gttttttcgt actcttgatt tatgcattga
    16141 ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc gatcaaatga cacaaatgct
    16201 tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac gagatattca tcgctcacta
    16261 tcaattaaca gataaagatt tccgtgtaca gactaatatt tatttcgata ccgttgatga
    16321 acagctgaag aaacaaaatt gtggcctacg gatccgtttc gttggtcatc aagctgaata
    16381 tacattaaaa acacctgctg aaaaagggcg tctcgaaaca acggatacct ttataacaga
    16441 agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt tcaggcgcag tttttcaaaa
    16501 actttctgat ttgaatatcg atcctgcttc attaatccaa accggaaagc ttacaacaaa
    16561 acgagctgaa ttccctattg aagaaggact tttagctatt gatgaaagct ggggagacaa
    16621 tctacacgat tttgaactgg aactagaggt aggggatgca tctgtgggaa aaattgcttt
    16681 tataaatttt ttaaaacgtt tttctttacc ttatcgtcct gctaagaaca agatccaacg
    16741 tatgcttgaa gctaaaaatt agaaaccttt cattttcaca aggcacttga gttcattttt
    16801 ttactttttt ctgttaagtt ataggatgtg aaagaatcta gtcactagtt gtatagaatc
    16861 tattttgggg gagattcata tgatcgaaat ctatttattt gttaatcctt taggggaata
    16921 ctgtttcgaa atggaacaac agttattaca atttatcgaa gaagagtacg ggacaccatc
    16981 aaaagaaaag atgcaatttc gctttcttcc tttggtcaat ttgcagacaa ttggggatgt
    17041 catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa cgtaatcact tattctcaat
    17101 gacttattct gcagcattgg actgcaaagc ggcgcagttt caaggaaaga aaaaaggccg
    17161 ccgttttttg atcaaattgc aagaagcagt cggctgtaat gacataccat attctcaaga
    17221 acttgctgaa tccatatttg aagaaatcgg cggcgatttg aatatgttta aagaagatcg
    17281 tcagtctgac ttcgttaaag aagtattctt ttctgatcaa aaaattgctc gggaaatggg
    17341 catcacaaaa cacccttctg cagtcgttta caattatgcc tgcgaccgtg attttggtgt
    17401 tttagtagaa gtaaaaggta caagtagcct ttggagaatc acagagctgt gtaaaacaac
    17461 agataactca tatcaagttt ttaatggtga acatttacat acaaatgatt ctcagttcca
    17521 ttcaagaact agccatttac acttgctttc taattaaaaa aggctct
//
LOCUS       DAYYCW010000059        17478 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000059 DAYYCW010000000
VERSION     DAYYCW010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.059"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            347..1072
                     /locus_tag="ACHAYA_001780"
     CDS             347..1072
                     /locus_tag="ACHAYA_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HFM8605576.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            1062..1736
                     /locus_tag="ACHAYA_001781"
     CDS             1062..1736
                     /locus_tag="ACHAYA_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFM8605577.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(2024..2308)
                     /locus_tag="ACHAYA_001782"
     CDS             complement(2024..2308)
                     /locus_tag="ACHAYA_001782"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HFM8605578.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(2602..3909)
                     /locus_tag="ACHAYA_001783"
     CDS             complement(2602..3909)
                     /locus_tag="ACHAYA_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM8605579.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            4398..5504
                     /locus_tag="ACHAYA_001784"
     CDS             4398..5504
                     /locus_tag="ACHAYA_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605580.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
     gene            6095..6295
                     /locus_tag="ACHAYA_001785"
     CDS             6095..6295
                     /locus_tag="ACHAYA_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8605581.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            6442..9231
                     /locus_tag="ACHAYA_001786"
     CDS             6442..9231
                     /locus_tag="ACHAYA_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM8605582.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            9278..9805
                     /locus_tag="ACHAYA_001787"
     CDS             9278..9805
                     /locus_tag="ACHAYA_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM8605583.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            9826..11016
                     /locus_tag="ACHAYA_001788"
     CDS             9826..11016
                     /locus_tag="ACHAYA_001788"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8605584.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            11056..12354
                     /gene="asnS"
                     /locus_tag="ACHAYA_001789"
     CDS             11056..12354
                     /gene="asnS"
                     /locus_tag="ACHAYA_001789"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM8605585.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            13288..13962
                     /locus_tag="ACHAYA_001790"
     CDS             13288..13962
                     /locus_tag="ACHAYA_001790"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8605586.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            13959..14300
                     /locus_tag="ACHAYA_001791"
     CDS             13959..14300
                     /locus_tag="ACHAYA_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605587.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            14330..14689
                     /locus_tag="ACHAYA_001792"
     CDS             14330..14689
                     /locus_tag="ACHAYA_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM8605588.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            14949..15071
                     /locus_tag="ACHAYA_001793"
     CDS             14949..15071
                     /locus_tag="ACHAYA_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605589.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            15213..17294
                     /locus_tag="ACHAYA_001794"
     CDS             15213..17294
                     /locus_tag="ACHAYA_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8605590.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
BASE COUNT         5812 a         3017 c         3629 g         5020 t
ORIGIN      
        1 cagatttttt agacctcgat catgatgaag acttttgtag atacaaataa ctgtaacatt
       61 gaaaatgaat gcgaaaacga tatttttttt tattggaaaa tatcggtcaa gcaataaacc
      121 agttgcataa taaaatgtta acggcaacaa aatctgtgga aggaatgaac cggatgattt
      181 gatggaaaaa acaagataga caagaagtat acatagtaaa acgtggaaca agtaggccag
      241 aatgaaaaat cagtttttat ccactcgctt ttctatacat tcactgtcac aaatataatc
      301 gaaagtaatt ctattttgag ataaagtatt ttataggaga tagtatatga acaaaaaaag
      361 atttaaacgt attgaaagaa aatttgcatt aacttatgac caagctaagt atttgaaaac
      421 actatttcca aaacaggtag ttcgggataa atatagtgat cattttattt cttccttgta
      481 ttttgataca aacgatcatc actttttaaa agaaatcact acagaaaagt cctatcgaga
      541 aaaatttaga atcagaaaat atggagatcc tcaggacata tcgcctatct acttagagat
      601 aaaaagaaag tttcaaggaa tgacagataa acgtagaata aaattagcgc atcatgagct
      661 cgatgatatg ttggaaaata tcgaattggc agaaaccaca ttcagtaatt tagaagaaga
      721 gtatattttt aatgaaatac agtggttgtt cagaaggttg gcattggagc ccaaagtata
      781 tatctattat ttaagagaag catttgtaaa tagagattca gaagatttgc gaatcacttt
      841 tgatcaaaat atccattacc gtctaatgaa cttaacgacc tgggaacact gtacagaaga
      901 caatctggta gcgccagaaa tcgatgtgct gatggaagta aaaacgacag gagaaatacc
      961 tgaatggtta caggtaggat tggataggat gggtaaaaaa gagataaaat tttctaaata
     1021 taaacaagtc tataaaagat atatccaaga ggacccgctc tatgttcaat agtgtcttaa
     1081 aaaatgagcc ttctcaactc acactagatg atttcttttt atgtatggtg gtgtcgattt
     1141 tgctaggcat tctgattgct tacacccata tgtatcaaaa caactgtaca aaaaacttca
     1201 tgtttgcctt gattgggtta cctactttag tacataccgt cattttgttg atcaacggta
     1261 acttaggcac ggggatcgct gttctaggcg catttaactt agtgcgattt cggtctgctc
     1321 aagggaattc aagagatatt ttcagtattt tttgggcaac gtcgattggt ttagccacag
     1381 ggacgggata tattgtctat gcactattcg tgtctagtat agttgctgct atccaagtca
     1441 tcatcttcac gcttccaatc aaaaataaac atacagtttt taatactgaa aaaacgatca
     1501 agctaaaagt ttctaaatcc ttgtttcttt cgagtattat tgaagaaaaa attgcagaat
     1561 atacagaaaa ctgcaattta aaaaaagtaa aaatcaataa agaaacttgt tccttacact
     1621 atatagtaaa agtaaaaacg agtatgcttg aaaaagaatt ggttcgaacg atcatggatc
     1681 atccagaagt catttcgatc actgtaggta tggataaatc gaataaatta ttgtgataac
     1741 caagaaagta cttaacaatg aagtaagacc ccagttccaa agtttagcgg ggacacttca
     1801 ataaagtact ttcttttttg tcttcagcaa ctactggtaa aactgagagc aagctttcgt
     1861 gagagtacag cgataaagat caacctccct ttttatctcg agcaatttcc ttgcaaggct
     1921 gtttcgttga tcttaaacga gttagcaaca gtcaaagatg cgatggccat aaaaaaattg
     1981 cattaagttg gctggtatct gatagctggg aatctaagga agactatcca ttctgtttct
     2041 taaattccgc tagttgatag atatcagcca attgttccag atgatcaaca tcaaagataa
     2101 ccgaaagatg tttcctttta ttcccatttg cttgaagagt ttttatcttc tctttggttc
     2161 cttcactcaa tgagagtgtg actaatttct ccctcgtttt ttctgggaaa tgaaaggata
     2221 ctttgaaaaa tccgtcccaa atagaaagcc agaagatcgg ttttactttc tgagagtggg
     2281 acaaaagtaa gaaacacttt tgtctcattc tttaaaaacg aataaacggt gggagcagaa
     2341 gcaactcctt cggaaataag ccaaaattca caaaaatttg aaaagcaatt ttcgtgaatt
     2401 tcttcttatt tctcggagtt aaacacttct gtcccaacct cttgtattca ccttttgata
     2461 gccaagcttt tccatcatta taaaaccgca aatttcaaaa aaaaacgaac aatacaaaaa
     2521 gcgtattgct cgtttttctc ttcagtttct cacacctaaa cgacttttgt ctcgacctct
     2581 ttttggtgac tattcatgat gttaaaataa attactgaaa aaatctgtga catggcgcca
     2641 accaatgaca aaaatattag ctttttcaac agtagaagtg gtaatgatgt ctgtcgaagg
     2701 ctcttggttt gcttccaaat agcctaattt gtcatcagct aattggatcg ttgctgagcc
     2761 aactgtagtg cctttgttga taggagcggt cacttggtca tccgttactt tctttttatc
     2821 aattgttgga gtaatcgtta attttccagt atccattcca ttttcgaccc agacttttac
     2881 agggcttttt agcgcaacat tgacggaggt ttcttttcca tctttcactt tgatgtcttt
     2941 taggcttggg atgcttgcat ttgcttttcc aagttctttt tctgaccagt tgtcataaca
     3001 ataatccatc aatcgtgatg tttcattgaa tcgggcactt ggattttctt catggttatc
     3061 tgcatttaaa acgactgtga tgattcgtcg gccatctttg ttgatcgttc cgacaaagca
     3121 tgcacccgca aaatcagttg tgccagtttt taatccatcg acaccttcgt gatagtttac
     3181 aaaaccaggt agcatccagt tccaattgac catttcaacc ggtgactgtg tgttttcgcc
     3241 gaatgtttga tttgtggtac tcgtgatttt taagacttca ggataatcta agatgaggtg
     3301 acgagcaaca atagcgacat ctttcgcaga cagttgattt tcatctgttt caccagtatt
     3361 ctcaggtcga ttctcaccta ggtaagaatt gtttaaacct gatgcattga cgatcgttgc
     3421 atctttgata ccccaatctt ttagttgttt cttcattagc tcaagaaatt tactttcact
     3481 gcctgcaact ttttcagcta aagcaaccat ggaagcattg gctgattgga tgaaagctga
     3541 gtcaacaagt tctttcactg tataatcatt ttctttatgc aaaggaacat tcgatagatc
     3601 cggcacgaca cttaattttt ctgcataatc cgaaatggct accttatctt cccaagataa
     3661 tttaccctct ttgatttgtt caagtacgat atatagacca atgatctttg tgatggaagc
     3721 aattcccatc gttttttcgc catcctgatc ataaaggatt tttccacttt ctgcatctac
     3781 ggcaaaggca gctttggcat ttattttaaa tgcgtcgtct gcttgaacag ttgcgctagg
     3841 caagaaagaa gtgatacata agataagact tgctaatagt acaataaaac gggtattttt
     3901 tttgaacatc tttgttctcc tcatttttaa attgattctt acaacttaga atattatcag
     3961 agagaacaaa aggtttcaat ggttctgaaa aaaaccttat aaagaacaag tcttattaat
     4021 gaagaatcat agaaagttat cttttgggtt cattgacacg aactttgact ggctgaactt
     4081 cgggttcggt taggcgtgat gctgcaaaaa gggcgcctgc tgagagtgcg accaaactaa
     4141 aaatcatgaa taattgcata agatcacctc gttctttttt gtttttttat agtatagcaa
     4201 gagagtttga agctactgtt aaggaaatga gcatataatt gatgaaagta tgaaaaaaat
     4261 atgaagtagg acaacacaac taaaaatcga tcaggtattt ctctggtagg gattcgtttt
     4321 attgtattga taaaatggga tgtggtagac tttatttaca gatacaaaga aatgattttt
     4381 ttaggagggg tacgtttatg gcaaaaaaag aaaaagaatt attatcacaa ttgacttcag
     4441 aaggtaccaa aggaccgttt gtcacgatta tgttaaacac ccatgttgcg catcaagacg
     4501 tagaaaaaga tcaaatcaaa ttcaagaatt ttgcaaagga agcgaaaaag cgctttgaaa
     4561 agaaatactc tgagcatgat tggtctccat tccaagaaaa aattactagt ttgttggacg
     4621 atccgtcttt ctggcgtagt gccacaacga gtgtagctgt catagtatca ccagaacaag
     4681 tgtttatcca ccgtttgggt attcctgtag atgaccagta ttatgtgagt gatctacctt
     4741 atttattagc gatcatcaaa aatggacatt tcaactattc atactattta ttaggattga
     4801 atcgtgattc gatgaaattg tacgaagtaa cgaataaaca agtcaaagaa gtggctttgc
     4861 ctgaagatgc accaagagat gtcgtcactg ctttaggatc tgaactaaca ggaggcgggt
     4921 tgaactacgc atcaaatggt agtgaaggta cgacgttcca tggagtcaat cctaaagatg
     4981 aagaagtcga aattgattgg gtcaattatt atcaagcagt cgatgactat ttgaaagata
     5041 catttgaaaa tccagagaaa tggcctatct tcttatttgc attaccagaa aatcagacga
     5101 tgttcaaaaa agtggcgaag aacccttatt atcgttccga tgttgcgatc tcagcttcac
     5161 ctgcacaact ttcattaaaa gaaattgaag caggcgcaaa aaaactatcg caagaattag
     5221 ctgtcaaaga agcaagtgac tacaataagt tgcttgataa aaagttcatt gatcaactcg
     5281 ttgatattat accggcagca aaagaaggga aaatatccca tttattcatt gctacatcta
     5341 atttagcaga tggttttggc gaagatccag aaacagaata tgaccgccgt caggtgctga
     5401 atgaactagc agataatgtg atcacgaacg gtggtcaggt ctttatcctt gaacaaaaag
     5461 atgcaccaga cgaaaagagt ttagttgcga tcttacgtta ttgagtgaaa actaccaaag
     5521 tagatgaatg gtcaggcgtt gatagaagtg tatagctccg agagaaggag tttcctctgt
     5581 ttcagccgtt ggattcatct tgagcgagag acaaaagcta gcttttgtct ctcgctcatt
     5641 atttttgtgc tttagtccca ttggctttgc gaggggaaga tacttttctc aaaaaaacga
     5701 accttgcagt tgagaggggg atagatgcgc atactaattt ttatcagtta aaaaatgatg
     5761 tggatcatta gagaattcca aagttcgttc atcctttttc agataataaa ttgaaatttt
     5821 ttgcaaaagt gaaaatagca aatagaaatt ttctgaatct tataggggtg atttcgagaa
     5881 aacgttcgtt tatttgcaaa tatatcttct aaataagaga ataaatacaa aaaaataagg
     5941 aaaaactctg gcaattttgt aaacggtatg atatagtaag aatgtagttt ttgttcggta
     6001 atctgtggta gcttgtttca aataaacatc ttccagatat acaccattca agtaattgca
     6061 aaatatgtgt tgatacacaa ggaggatttt tattatggaa acaggtacag taaaatggtt
     6121 taactcagaa aaaggatttg gattcatcac tgcagaaaac ggaaacgatg tattcgttca
     6181 tttctcagct atccaaggcg acggattcaa aactttagaa gaaggccaag cagtaacttt
     6241 cgacgttgaa gaaggtcaac gcggacctca agcaacaaac gttactaaag cttaatttac
     6301 ctatattaaa aataaaagtc cagacattta tgtgtctggg cttttttgtc tttttttcct
     6361 taaaaagcga tgagagtatt tttctgcact ttgtagtata atttcaaaag aacattagag
     6421 gcaggagaag aggaagatat tttgaaacca agaacttatg cagtcgttga tatcgagacg
     6481 actgggacga atccaaaaga agatcggatc atccaatttg gctgcgtcat gattgaagct
     6541 ggtcggatca cttcacgttt ttcgatcgat attcatccag ggcggaagat ttcaaaacaa
     6601 atccagcatt taacagggat cacgaatcaa cgcgtccaaa aagcgcctta ttttgaagat
     6661 gtcgcccaaa cgatttacaa tcttttggca gacaccatat ttgtcgcgca taatatttat
     6721 tttgattata attttttgaa ccatgaactc atgcgatgcg gacttcctag tttgaagata
     6781 ccgggcatcg ataccgtaga acttgcacaa attttcttgc cgacggaacc aagttttcgc
     6841 ttagctgatt tgtcagaaag ccttggcttt attcatgaga atcctcatca ggcggatagt
     6901 gatgccgaag tgacaggtca gcttctttta ctgatcgaag aacgaatgag gaaattgccg
     6961 atcatcacat tagagcagat tgcacgatta agtagccata caggaatgga tacgagtcgt
     7021 ttcatttacc acgtgcttga agaaatgaaa gaaaacccgg agccccttga tctctcatta
     7081 gaaatcatcg atgggctagc tttgcagaaa aaagaagtgg agctattcac atccgtccat
     7141 tatggcgagc ggacatatcc aagaaaaaaa caagcaaaag aaaaaatgtt tggtaaaaca
     7201 ctgatgtatc gaaaagaaca gaacaggctg atgaatgctg tctacgacca tttcacaaaa
     7261 gatgaatcaa aagatttgat gatcgaagca gctacaggaa tgggcaaaac gattggttat
     7321 ctgttgcctt tgagttattt ggctacacct gaaaaaccag ctgtcatcag caccgtttcg
     7381 cttgttttgc agcagcagat catggaaaaa gacattccat tactgaatca attgctagat
     7441 cagccgatac aacctgtgat tatcaaaagc tatcgccact atattgattt acagcgtttt
     7501 aaaggtacac ttgtggaacc gccgggacaa aaacaatatg ctttgtacca aatggctgtc
     7561 ttagtttggc taacacaaac gaagaccggt gatttggatg aattacattt aaccaatctg
     7621 aaccatgcat tctttgcaga tgtcgcgcac agaggcactg ggtttttagc aaggaatcag
     7681 tcattttaca aacaagactt tgtgcgtttc ttgcaaaaga aaatcaaaca aagcaatttt
     7741 ttgatagtca accatgcatt tttagtacaa gaaacacagc gaaaagaacc attgcttcca
     7801 gctagtcctt atttgttgat tgatgaagtg catcatttgc cggaaattgc tgaaaaagta
     7861 aatgacaaac gactaagtat taactacttc cataaacaaa ttcagcaact ggaagagccg
     7921 aatcagttat ttgaccgcat acaaagttat ttgccgaagg aacatgaagc cttgcgctgg
     7981 ttgtcgattt atcgagaaga actctctgca ctatgcgaag cgcagatttg cgtggcagag
     8041 ggattaatga acttagtaag cagcaagcaa cctaacggac aatatccaga agaagtcatg
     8101 atgacaaaag aaatgttgga tcagctgcca ctggaatctg gaacagcaat ggatcatctg
     8161 caactttttt atcaagagat ccaacaacta caatcacaga tgcgcttgac ttttgagatt
     8221 atccaagaaa gttggaccaa ccagatgcgg atcgatttag caagtttata tagcttattt
     8281 gatcaattag acaagcaata cttgctgatg gaccaatggc tagaagactg gcgagatcat
     8341 ttgatccatt ggttcgttcc cgggaataca gcacagcaga tgatttttca tctccttgat
     8401 tttcaagcag ctatgttgcc gtcaactgtg tggtatcctc gatatgacca tattctttat
     8461 attggaggaa cactgaagat cggacctaat cgacagtatc tctctaaacg acttggcatt
     8521 gaagcagcac ctcttaaagt gattgcttct ccttatgatt atgagaaaca ggcaagaatc
     8581 tatgtgccga aagaagcacc tgcagtccat caagtcagca atcaagcata tatcgattat
     8641 ttagaagcag cactcacagg attgattgaa caagaagatc ggccgatcct tgttttattc
     8701 acttctcatg atgttttgca aaaggtatat cagaaaatgc atttgtctat gctagaaaaa
     8761 ggacgtgaaa ttcttgctca aggatttggc gggagtcggg aaaaactttt gaaacgcttc
     8821 attttgtcag aaaaaagcgt cttatttggt gctgacagct tttgggaagg cgtagattta
     8881 cctggagatg ctttgcagat tgttgtagtc acacgtttac ccttcgacaa cccgttacgt
     8941 ccgatggtca aagctaggaa tgcttatctg gaatcagagg ggatcaatcc tttttatcag
     9001 gaatccgtgc ctaaagcagc tttgaagtta cgccaagcat taggtcgttt gataaggacg
     9061 gagacagatc gtggcgtatt acttattttg gatcggagat tagtcactgc aaaatatggt
     9121 gcacgaatca tccatgcatt accgaaaaaa gtaccgataa aagagctagc tatagatgaa
     9181 atctatcagg atatccatga attcttaaag aaaaatgaag aaggaagtta aaggtgccgt
     9241 cttttttcat cattaaaatc tgctataatg aaaaggaatg aagataaagg agcgacgacc
     9301 cttgaagaaa aaacagacaa aggaaaaaac gattaatcgc gtgttgcttg gcttgatcgt
     9361 atttttatta gcggtcatcg tgatcagctc gattttttac ttgcgttcca gtcgtccggc
     9421 ggtccaggcg aaaaaagaag cagtagaaat cgcaaagaaa tatgcaaatc tcgaggaagc
     9481 cgatcatttt tactggttta ctcgaaagaa aacgtacttt tcattgacgg gtacgaatga
     9541 taaaggaaaa gaaatcgccg ttatcattcc taaatctggt gaaaaagtaa aagtattgga
     9601 tcaaacaaag ggactgactg aaaacgaagc aaaacagaca attgctgcgg cccatcctga
     9661 aatccaaatc gaaaaagctg ctttagggat gtatgatgat cagcctgtgt gggaagtagt
     9721 aggaaaagaa acaaatggga aactgaatta ttacctgatt ggctttgaat ctggagatga
     9781 agtcaagaca atcaagggaa tttaaaaggt aaaggaatga atgggatgga gttatcaaaa
     9841 agaacaaaac gattagaacc gtctgtcact ttggcagctg cggcaaaggc caaggcatta
     9901 aaagcaaaag gcagagatgt gctgagtttg actgtgggag aaccggattt tgcgacacca
     9961 gaaaacatcc aagaagcagc aatcgaagca atccgcaatg ggaaagctag ctattataca
    10021 ccaacagcag gaatcccaga gcttcgacag gcaatcgttg attatctgaa gaaaaactat
    10081 ggacttgtct atgaaccttc acaaacagtc gtaacagacg gagcgaaatt tgctttgtat
    10141 acccttttcc aaacgatcct tgacccgcaa gatgaagtca tcattcctgt gccttactgg
    10201 gtcagttacg gtgagcaagt aaaactggca gaaggacttc ctgtctttgt gaaaggggaa
    10261 gaatccaata gctggaaagt tactgttgag cagctagaac aagcacgttc agaaaaaaca
    10321 aaagccgtaa tcatcaattc tccatcgaat ccgactggaa tgatttatag caagaatgaa
    10381 ctgcaggcaa tcggtgaatg ggcagtaaaa aatgatattt tgatagttgc tgatgatatt
    10441 tatggccgtt tagtttataa tggcaatgag tttactccaa ttgcaacgat ttcagaagca
    10501 atcaaaaatc agaccatcat catcaatggc gtgtctaaaa catatgcgat gacaggctgg
    10561 cgcattggtt atgcggtggg gaattctgag atcatcaatg gaatgattgc tgttgcttcc
    10621 cagtcaacca gtaatccgac agcagtcagt caatatgcag ctgttgaagc attgaaaggc
    10681 gagcaagata cagtggaaga aatgcggaac gcctttgaag aacgcctgaa tacgttatat
    10741 ccgcttgtgg cagaactgcc gggtgtttct cttgaaaagc cacaaggagc tttttatctt
    10801 tttcctaatg taaaagaaac gctacgcatg tgtaaatatg aaaatgtcac acaatgggta
    10861 gaagatcttt tagaagaaac aggggtagcg cttgtgactg gcgaaggctt tggggcacca
    10921 gaaaatgtcc gcatgagtta tgccactgat cgcgctacgt tagaagaagc ggtacgcaga
    10981 atcaagcaat ttattgagag taaaagtcaa aattaatcct gaagaaattc aaggaatata
    11041 aaaggagagt catacgtgga acaaattcaa atcatcgatt cgaaaaacca tgtgggagaa
    11101 acagtaacaa tcggcgcttg ggtcgcaaat aagcgatcaa gcggaaaaat cgcatttttg
    11161 caattaagag acggaacagc ctttttccaa ggtgtagttg taaaaagcga agtgccggaa
    11221 gaagttttcc aaacagctaa aaatctacac caagaaacat ccgtgttagt aactggagaa
    11281 atccgtgaag acagtcgttc aaaatttggt tatgaaatcg gaattacaaa tgtggaagtg
    11341 gtaagcgaga gccacgatta tccgatcaca ccaaaagaac acggaacaga cttcttgatg
    11401 gatcatcgtc atttatggct gcgttcttca agacaacatg cgattatgca gatcagaaat
    11461 gaaatcattc gtgcaacgta tgaattcttt aatcaacatc attttgtcaa aatcgatccg
    11521 ccgatcttga ctggttctgc accagaaggc actacggatc tattcgaaac aaattacttt
    11581 gatcaaaaag cttatttatc tcaaagtggc caattataca tggaagctgc tgctatggct
    11641 ttcggaaaag tcttttcatt cggtccgact ttccgtgctg aaaaatcaaa aacacgacgt
    11701 catttgatcg aattctggat gatcgaacca gaaatggctt tcactcatca agaagaaagt
    11761 ttagaagtac aggaacaata tgtagcattc ttagtacaaa atgttttaga tcattgcgaa
    11821 tacgcattag atgtactagg acgagacaaa gaagtcttaa aaacttatac aaaattgcca
    11881 ttccctcgta ttacttatga tgatgcgatc gaattactga agaaaaatgg atttgatgac
    11941 atcgaatggg gcgaagactt tggttcacct catgaaacgt tcattgcgaa ttcattcgaa
    12001 cagcctgtct ttatcctaaa ctatccaaaa gccatcaagc cattctacat gaaaccacat
    12061 ccaacaagag aagacgtggt gatctgtgca gatatgatcg caccagaagg ttacggtgaa
    12121 attattggtg gaagcgaacg ggcaacagac tatgatttct tgctagaaca aatccgtaat
    12181 cacggtttgg atgaaaaaga atattcatgg tatcttgatc tacgtaaata cggttctgtt
    12241 ccgcactctg gttttggtct aggccttgaa cgaacagtca cttggattgc tggtatcgac
    12301 catgtgcgtg aagctattcc attcccacgt ttgttgaacc gaatttatcc ataagaggtt
    12361 gtgaaatcag tgccttgacc tgagtattag agaaacaaaa cggaaaaata gcttctcata
    12421 ttttactgtt ttttgagcta atatcgaggg tcagctgatt gaacaccgtt tatatcaaga
    12481 ggttgtgcgt ctgccgttca gctccgagtc acaaggagta atttcaaaaa aacatttttt
    12541 catgtttgat gactattgtg acttgtgacc aaggagttgg ccggagagca acgtttattt
    12601 aaaaaaaaga actgcgaact ctcgcagttc ttttttttta aatacgggat aaaggaagtt
    12661 tccagcagat aagagaagaa agcaaaagaa attgctattt gattcacaag gttctataac
    12721 gaaatcgcaa tcaatcaaac atatacaaag aaggttattc aaatggaaat ctgatttgat
    12781 gatgcttctg ctgttgaaat aaagcagcat ctagcacctg aaaacaaact aatagtaaca
    12841 ttcgaagatg gagtaggtcc gtattttcag catgcaatga tccatatgta agttcaattc
    12901 acccttgaaa tcatcggacc agataaagtt atggccaatc tatcccaagc aatctagaag
    12961 agatattagt aaaggaacat ttactggaag tcttagatga gcctataaat atatgttctg
    13021 atggcgtcat caagtggtct gattgacgga agcttaggtg tgatcgatct ctctggacca
    13081 aacaatgaag gaatcaaggg gaatccagca cgttaagaaa aaaatagcat aaaaaactag
    13141 acacttgatg agcgttcatt caattgtttg aagggactag gctggtgtca ttttttatac
    13201 atatattttc tctttcgtct tgatagggct tatgggataa tataaggaag aaaatcatcg
    13261 ttttattcaa aaaggagttt ttgacaaatg aaaaaacgta tattatttat cggtgacagc
    13321 attacagacg caggaagaga ccgagcagaa gcaactgatc taggtaaagg ttatccactt
    13381 ttgattgccg gaaaactaat ctcccaatat ccaacgatgg aatttgaatg catgaaccgt
    13441 gggataggag gagacaaaat tgcagatctg caagtacgct ggcgagaaga ttgtttagct
    13501 ttacaacctg actatgtatc gattttgatc ggaatcaatg atacctggca taatactagc
    13561 gatcaggcta tattcggaac tgagaaatct gctcagcaat ttgaatccgt ttatcgaaaa
    13621 ttactaaatg aacttcaaaa agcgggaatc tgcaacatca tcttgatgga gccgtttgta
    13681 cttcctataa ccgacgaccg tcatggctgg cgtaaagact tagaccctaa aatccaaatt
    13741 gttcgtaaac ttgctaagga atatggcgca ttgctggttc ctttagatgg catattcaat
    13801 gctttaggga ttgcttatgg tttttcgaaa tatacaagag aagatggggt ccacccaacg
    13861 attggcggac atgagatcat tgcacaaagc tggatagatg caattgccgc agtcgatttt
    13921 tttagtggag ataaaaatga gcgaaagaaa agtgagaaat gatggaaata cggtgggcag
    13981 taaaagaaga tctacctcaa atcaatcagc tgattactga tcatccagaa tcattcgtaa
    14041 aagcaaaaac ggcagctgac ctaaatgata agaataaact attgctcgtt gctgtaacaa
    14101 aagaaaaacg gattatcggt tttgcgcaag cggacgtgca acagtcagaa gaacgggtgg
    14161 gtcagcgacc atcaggaaaa ctaatccaac acacattagt aacaaaagaa aatacggaag
    14221 aaatccaaaa aaaatttgca gaatatttga tgctggaagc taaaaaaaga agaataaaat
    14281 caatttttgc ccagttttaa tcaaaaacag taatagaagg agaaatacaa tgagttgttt
    14341 aggaaatgtc atctggttta tattcggcgg actaatcggt ggtcttagct ggttagcagc
    14401 ggggatactt tggtgtatca cgatcatagg tatcccaatc ggtatgcaat gttttaagat
    14461 cgccttcctt tcttttgcgc cttttggcaa agaagtaatt tatcaccagt ctggcatgtc
    14521 attgatcgtt aatatcatct ggctgatcgt ttcaggccta ccaattgctc tcgcacatgt
    14581 cgtcagcggc atccttctat gtatcacgat catagggata ccttttgcca agcaatcttt
    14641 caaacttgct cgattggcgt tgatgccatt cggtgcagaa attgtataaa agagaataat
    14701 aaaggttaga taaagcatag agtcaggtgg gatacatgta tttcacctga ctctatgcgt
    14761 tttcctgtat aagttcttcc atgtgacttt tacgtctttt atttatatgg ggttactgtt
    14821 tatggaaagt tgaaataatt ttcattaaag aactctttct atatttttga aagcgtagtg
    14881 gagcttcgta tatctgggaa ggacattttg cagggattga aaaaagatat tgtcctatcg
    14941 aaaaattaat gaatttagac aaattgccag caacaggctt caaacttagt tgctatcctg
    15001 tcaagatcaa gaaggcaagt gcgggttgga taagagcagt ggcaatgatc gaagagaaaa
    15061 agaaagaata agccaaaaca tctgtatgga agaaattttc cgatgcagat gtttttcatt
    15121 ttttctttcg gataccttga catatgaata accattcatt tataattagt tatgaagaga
    15181 tattcatatg ttgaggcgag gaggtcatta aaatgaaaag ttacagatta gaaggtttag
    15241 attgtgcgaa ctgtgcaatg aaaatcgaaa aaggagttca gaaaatcaat ggagtcaagg
    15301 aagcaacggt aaacttcact tctggaaaac taacgattga cgcggaggaa gatcatttgg
    15361 ctacgattga acaagaaaca aaaaaagtag tgaaggaatt agaaccagat gtcaaagtaa
    15421 cagaaataga taaggaaaaa gtatctgaac atggaaatga aaaagagaga aatacgctgt
    15481 tccggatttt attttccctt gcaggtatcg ctttattgct attatttgat tttaatgaac
    15541 taattcgctt gatagggtat ttattgattt atctgctgat aggatatgat gtagtgaaga
    15601 aggcagtgat gaatatcgtc aaaggaaaaa tctttgatga gaattttctt atgtctgttg
    15661 ctacgattgg cgctatgttg atcggtgaat atccagaagc cgtagcagtg atgctatttt
    15721 atcaaatcgg cgagtatttc caaggattgg cagtcagtca ttcaagaaaa tcgattcgcg
    15781 aattgatggc gatccgtcca gaaacagctc acgtgcagac ggcggaagga ttgatgaccg
    15841 tgaaccctga agacgtgctg atcggtcagt ttgtacttgt gaagcctggt gaacgtgtac
    15901 cgttagatgg aacgatcatc gaaggagaat cattagtcga tacgtcagca cttacaggag
    15961 aatcagtccc taaatctgta tatgttggtg aaacagtatt aagcggattt atcaataaaa
    16021 ataaaccatt tttagttcaa gtagaaaaat cttatgaaaa ttctactatc agcaaattat
    16081 tagaattagt agaaaatgcc agcagtaaaa aagcaccagc tgaaaatttc attactaaat
    16141 tcgcgcgcta ttacacacca gtcgttgtag gattggccgt tttattagct gttttacctc
    16201 cacttgttgt ttctggagcg gcgttttctg aatggatcta tcgagcattg acatttttag
    16261 tgatctcatg tccatgtgca ttagtgattt ccgtgccact tagtttcttt ggcggtatcg
    16321 gaggagcaag taaaatcggt gtattagtaa aaggttcaaa ttacttggag ttgcttgcgc
    16381 aaaccgagac agtggtattc gataaaaccg gcacattaac gaaaggcgac ttttcgatcc
    16441 agacgatcga tacaaaaatc gatccaaaga tattcatgca atatattgcg agtgcagaac
    16501 agttttctac acatccgatt gctcaatctg ttttagaagg ctacgcaggt ccgcttttac
    16561 caacagctaa tatccaggaa tttgcaggtg aagggatctt agctgaagtt gatggcaaac
    16621 aagtattagt aggtaatcac aaattgatgg aaagatttga aatctctttt ccttcaagtc
    16681 aagaaatcgg tacattgctt tatctagcaa tcgatcaaag ttacagtggt tatcttgtta
    16741 ttgcagatac cttgaaagaa gatgccgtag acgcattggt tcaattaaaa caggctggtg
    16801 tgaaaaacac agtcatgttg acgggcgact cgaaaaaaat tgctgatcat atcgggaagc
    16861 aagtaggcgt ggacaagata tatagcgaat tgcttccaga agataaagtg cagcggttag
    16921 aggaaatctt acaaagtaac aataaaaaga ccgcatttgt cggagatgga atcaacgatg
    16981 caccggtgct agcaagagca gatgtaggaa tcgcaatggg tggattggga agcgatgctg
    17041 caattgaagc agcagatgta gtcatcatga atgatcaacc atcgaagatt gcagaagcaa
    17101 tccatttagc aaagaaaaca ttgaaaatcg tcaaacaaaa cattgtcttt gctattggga
    17161 taaaaatttt ggtactttcc ttaggtgctt ttggttttgc atcaatggga gatgcagtat
    17221 ttgcagatgt tggggtgaca gtattagctg ttttgaatgc catgcgaagc ttgcatgtaa
    17281 aaaataaacg ttgatcaatg aagaggacga atgaacgtcc tctttttttt atatcctttt
    17341 cttaatgaaa aatgtaaaga aaatagatag gattataaaa taactaatgt ttttaatttt
    17401 gcagaaaatt caaaatagct tgaaattcaa taaggtcaat gaaataaaag aaaataactt
    17461 atttttttga tggaaaaa
//
LOCUS       DAYYCW010000060        17317 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000060 DAYYCW010000000
VERSION     DAYYCW010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.060"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..573)
                     /locus_tag="ACHAYA_001795"
     CDS             complement(28..573)
                     /locus_tag="ACHAYA_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8605591.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(585..1277)
                     /locus_tag="ACHAYA_001796"
     CDS             complement(585..1277)
                     /locus_tag="ACHAYA_001796"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM8605592.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1296..1580)
                     /gene="macP"
                     /locus_tag="ACHAYA_001797"
     CDS             complement(1296..1580)
                     /gene="macP"
                     /locus_tag="ACHAYA_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM8605593.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1593..2156)
                     /locus_tag="ACHAYA_001798"
     CDS             complement(1593..2156)
                     /locus_tag="ACHAYA_001798"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8605594.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2384..3046
                     /locus_tag="ACHAYA_001799"
     CDS             2384..3046
                     /locus_tag="ACHAYA_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM8605595.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3170..4366
                     /locus_tag="ACHAYA_001800"
     CDS             3170..4366
                     /locus_tag="ACHAYA_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM8605596.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4467..5843)
                     /locus_tag="ACHAYA_001801"
     CDS             complement(4467..5843)
                     /locus_tag="ACHAYA_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8605597.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5880..6747)
                     /locus_tag="ACHAYA_001802"
                     /pseudo
     CDS             complement(5880..6747)
                     /locus_tag="ACHAYA_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
     gene            complement(6836..7861)
                     /locus_tag="ACHAYA_001803"
     CDS             complement(6836..7861)
                     /locus_tag="ACHAYA_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8605598.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7879..8553)
                     /locus_tag="ACHAYA_001804"
     CDS             complement(7879..8553)
                     /locus_tag="ACHAYA_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8605599.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8737..9630)
                     /locus_tag="ACHAYA_001805"
     CDS             complement(8737..9630)
                     /locus_tag="ACHAYA_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8605600.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9704..11401)
                     /locus_tag="ACHAYA_001806"
     CDS             complement(9704..11401)
                     /locus_tag="ACHAYA_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8605601.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11394..12707)
                     /locus_tag="ACHAYA_001807"
     CDS             complement(11394..12707)
                     /locus_tag="ACHAYA_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8605602.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12707..16648)
                     /locus_tag="ACHAYA_001808"
     CDS             complement(12707..16648)
                     /locus_tag="ACHAYA_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8605603.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16757..17215)
                     /locus_tag="ACHAYA_001809"
     CDS             complement(16757..17215)
                     /locus_tag="ACHAYA_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8605604.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4856 a         3893 c         3010 g         5558 t
ORIGIN      
        1 tttattttat caaatcactt tgttttttta taaaatttct gcgcctagtg tggactcaaa
       61 atgtctcaat gcccattcat gtccaacggg atcgaaactt gccacggaat ctttgaaaat
      121 atggagtttg tatcctagat tatacgcatc gactgctgta tgcaacacac agatatctgt
      181 acatacgccg gtcaaataaa tatctgtgat ctggcgttct cgcaaacgga tgtctagatc
      241 tgttccacta aaagcggaat aatgccgttt gtctatccaa taaacatttt cctcttgctc
      301 atagtcttgg tataaacttt ttaaagagcc gtacaattcc cgtccacccg ttcctatcac
      361 attatgcgga ggaaataagc ggttctccgg atgaaaggaa tcttttggat catgcgcatc
      421 aatggcaaaa acgacaaaat ccttttgatc aataaacttc tttgtatgag cagtcagctt
      481 tttttcaatt acttgtcctg ctgtcccagt agtcagcttt ccatcctctg caacaaaatc
      541 atatgtgtag tcaatcgaga tcaatgcgtt catcttagtc ctccttatac gagatgtttg
      601 acaaattcga tcaccatttc tgctgatcgt ttgcctgcgt cttcgataaa ttcgtcaaag
      661 ctttgtgtcg ccgagtgatc tgctgtgtcg ctcattgcac gtacgatcaa aaatgggata
      721 ttgaactgac gagctgtttg tccaacagcg gctccttcca tttcgcaagc taatgcttca
      781 gggaaattcg ttaagatttc tttgatttta tctggcgagt cgacaaatgt atcacctgta
      841 acgatcaatc cttcttttgc gttcaaattc gttgcttttg ctgctttaac catttcactg
      901 cgcaaatatg tgcttgcttc ataatataga ggcattcctg gtaattgtcc tgggttgtag
      961 ccaaatcctg ttgcatctgc atcaaaataa gctactttgt ctgatataac gatgtcacct
     1021 acttgtaagc cttcaccgat tccgccagct gaacctgtat tgatcaccat gttcacgccg
     1081 tattgttgga tcaacagact tgtggtaata gatgctaaga cttttcctat accggaacgt
     1141 acgacgatca cttcgtggtt acctaatgaa cctgaaataa ataaagcgcc tgcgcgttcc
     1201 catgataatg gttcgcttag gttctctctc aaaattttta cttcttcttc cattgcgccg
     1261 ataataccga ttttcatctt tttaattcct tctttctata taaatgcgat tgctaaaaaa
     1321 accacacata acattaggat aacaataata agagatttca tcaaaaaaga gttccatttg
     1381 gtcgtctttt cccgttcgct gatccttgtt ttcgtgattg gtttattctt tttgctttct
     1441 ttacgataaa agctggcaat ctttttttct tcttgctgat aagctgtttc tgcttttcgc
     1501 tgttttttta atgactcgct tgcttgtgct tgttgtcgct tgcgaagttc gcttcgcgta
     1561 ataagcggtc ctttggccat aagccgatcc ctctactttc ctttggtcaa tagttcccag
     1621 tattgaatcg cataaatcgt ctttgcatca caaatcaact gatctttcat cgcttgttgc
     1681 gcctcttcaa gagttagatg atacaactcc agtacttcgt cttcatcttg tgctaaagga
     1741 ttctcgacct tctctacccc ttgtgcatga taaatatgaa ggacttcatt tgcaaaacct
     1801 ggtgaaagat acatcgaagt aagatggctc agcgacttcg cacgatagcc agtttcttct
     1861 tctaattctc tagctgctgt catttcggga ttttgtcctt ctcctggatc gatttttcca
     1921 gctggaatct ccaaaatcac tttttctaaa ggttttcgaa attgtttgac aagaagcaga
     1981 cgatcttgtt cgtcaaaagc aatgatcccg acaccgccag gatgaaatac cagttctctc
     2041 ttcgaagttc ctccatctgg caaacgaact tcgtctacag caacatcaat aatttttccg
     2101 ctatagattt ctttgcgact gattgttttt tcttcaaatt gctcattgtt cagcatatct
     2161 tctcctgctt tctttgtaat cataactgct tttttttcat ttttcaattt ttctcaaaaa
     2221 ctccttatta gtttaccata gataaacctt agattttctc taaatataga aaatccttct
     2281 ttagtccgat actctttcta gtagtccgta tgctaaaata agtatgtaat cgaataacga
     2341 aaagaattca atactcaacg ataaatgaca tggagggttt attatgataa aacgaaattg
     2401 gccgtggctt ctagcattgg tcttgctatt tctctttgtc cgaaacaata gtttttcttt
     2461 tgttctagga cttttattga taggtgtagt tttagttttg tggggactct ttggttcgcg
     2521 caggaaaaag ggaaaacaag aagaaattcc tgcactgact gacgaacttg aaactcacta
     2581 tgaaaaaagc ggtatgacag cgaatgaaat ttcatttttt cgacaaacaa tgaatcaaac
     2641 aaaaaatgag atcaatcaat tgcaaatgaa tatgcagcag acagccaaac ttaaagcaat
     2701 cgatctccgc catgatccag tcaaagcagc gaaagcttta ttcaaagaat tggtgaaaga
     2761 accaaaccga ctgcatgaag caagtcagtt tctttatacg catctgccga atctagttga
     2821 tttgacaaat aagtataatg agatcaatga ccatgagatc aaaaacaaac agacctatga
     2881 aaaactagaa gaaagcgcgc agatcatcga ccagctgtca gctttaatcg cacaagatta
     2941 tcaaaaattc gttgctgagg atctagatga tatcgacgta gagatatctg ttgcgaaaca
     3001 gagcttaaag cgtgataata aatatgaacc agaacaaaag gattaactct tttgttcata
     3061 aaagaagact cggaacattc agatgatgtc tggctgtttc gagtactttt tctaaagtag
     3121 agacattcat gtaagaatcg agaacagatt caacaaggag gaatttttta tggatcataa
     3181 accgactgaa acaaaagatg ttacaccagt taacgataca cttgaagatt tattgaacaa
     3241 cccgttttca actccaacag atacgctgac cacttcacaa caagcggaga ttgatcagct
     3301 ccaagagcaa cagacagctg ctcgtttaat tgacaaacta ccggcggaac gccaagaaca
     3361 agctaaacag ctagcagcaa aaatcgatgc taatgatgca cagtctgtca tcagctacgg
     3421 atcggctgcc caagcaaaat taagtgaatt ctcacaatcg atgttgaatc atgtgcaagc
     3481 acaagatatt ggtcctgtag gagattcctt aactgaactg atgtatcgtc tccaagaagc
     3541 caatcctgac gaactgcgtg ccggagaagg gaacatcttc cagcgtgtct tcgggaaggt
     3601 caaacaatcg atctatgaag tgacagcaaa atatcaaaag attggtgcac aaattgacaa
     3661 gatttcagtg aaattagata aagaaaaaga cggtctgcta aaagataatc tgatgttaga
     3721 acagctttac cagaaaaaca aagactactt tgatgcactg aatatttata tcgctgctgg
     3781 agaattgaaa atggaagaac ttcaaacaac gatcattcca gaagcgatga aaagagcaga
     3841 agaaactggc gatcagatgg atgttcagat tgccaatgat tacacgcaat tcctagatcg
     3901 tctggataaa cgtacgcatg atcttcgctt agctagacag atcacgatcc agcaagcacc
     3961 acaaatccgt ttgatccaaa acacaaatca ggccttggct gaaaagatcc aagcatctat
     4021 cgctacagcc attccgctgt ggaaaaacca agtcgtgatc gcattgacct tgcttcgtca
     4081 aaaagatgca gtaactgctc aacgccaagt ctctgaaaca acaaacgact tattgaagaa
     4141 aaattcagaa atgctgaaaa tctcagccat cgaaacagcg aaagaaaatg aacgtggaat
     4201 cgtggatatc gagacccttc aaaccactca aaatgatttg atcgaaacaa tccaagagac
     4261 attgcgtatc caaaaagaag gaaaagaaaa acgtcgtcat gcagaagtag agcttggaca
     4321 catggaagaa gatctgaaac aaaaactgtt ggatttaacc caatagatca cctttcgctt
     4381 gttactaaaa caaacgccca acaacagttt attacaactg ttgatgggcg tttttcttat
     4441 ctttcttcta acttctccat tggagtctat ttctcattca tcttgctgtg tccatagcca
     4501 tagccaaaat aaatgatcag tccaataacc attacgatac cgaacacgat ccatgtcaca
     4561 gtttgcagac gaagcatcaa tacaatacaa gtcaaaatgg agacgattgg caatagcgga
     4621 acgaatggca ctttgaattc tccagctttt ggtttgccaa acatttttct caacttcagt
     4681 aagcctagac taacaagcat caaataggcg agcgccatga tattcgttag ttccgctaat
     4741 acattcaatg ggaaaaatcc ggcaaagaat acagctgcca caccagcggc ataagtcgct
     4801 tttttcggtg tacgatgctt ttgatcgatt tctgtcataa acttaggaag taagccatcc
     4861 ttgctgacag caaacagtaa acgagcaagt gaatacatca tcgagatcgt taccgtcagt
     4921 agcgttaaaa ttgcccctac agcgatcacc gcgcccactg ttccatgtcc tacatacctc
     4981 attgcaaaag ccactgggtc attgactcct agttcagtat acggaacgat tcctgtcaag
     5041 atcagcgtca cgatgatgta aaggacagta gcaatcagta tcgaaccaat gattcctctt
     5101 ggcacatctt tttgcgggtt tttgacttcc tcggctgcca ttgcaacggc atcaaatccc
     5161 aagaaagcaa aaaacacaag agctgcacca tctagcacac cggaaaaacc aaaaggcatg
     5221 aatggctgcc agttatcagg tttaacgaaa aatattccta caatgataaa caacgcgatg
     5281 ataccaaatt tgacatatac catggcatta ttcaaacgta atgctttctt ggcttcaagc
     5341 gacacgatgt aagtgacgaa tatcactacg agcaccgcaa tcaagtcaat gtatgtacca
     5401 ttttccggat tgtacccgcc agttaatgct tgtggaagtt cggcccctaa acttttcaaa
     5461 aaaccttgca tatagccaga ccatccggaa gcaacagagg aaaccgctag taaaaattcc
     5521 ccgatcaaca accaccctgt cagccaggca gcaaattcac caaatacaac atacagatag
     5581 gcatatggcc caccaatcac tggcactcgc gaagcaaatt ctgcgaaact tagtccagac
     5641 aaaatgacaa cgattgcagc taagacaaat gaaagtgaca gtgctggacc agcattttga
     5701 ttggccgcaa cccctgtcac cacaaatatt cccgtaccga tgatcgcacc aattccaagc
     5761 atgatcaaat cagacgtttt tagttctttt ttcatctcac tcgtatgatt gagattgatc
     5821 tctttcttac gaaacaaatc cattgaagtt cctccccatt ttaggttgga aaacagcgat
     5881 taaactgttt tcatataccc taaaagggat accacctaaa atgactaaaa ggcaaccaac
     5941 aataacataa atgagctctt ttttcgtttt tctttcgcca aggatgaaaa tcccacctaa
     6001 agtagagata atgatcccca tttgtgaaag tgagaagcca acagctaaac caagtgattt
     6061 gactgttaga agcatgcaga tgtttcctaa gccccataat aatccacaga tcatgttttt
     6121 ccacacaaac tgatcgactt taactttacg gaaagcaaag aagcttgcgc ctaagatcat
     6181 tccgatactt tgaggtaaga taattgacat cgcatccaaa ttcgcccagt tgacgatgat
     6241 ggtatataca ccataaccaa tcgtagagaa gatcaatgct ctaaaaccct taccaaaatc
     6301 aagcatttta ttatctgtat ctatttttcc ttcgtcatct tgtcgggcag tcaaatacgc
     6361 accgcttaca agaagagcta aagcaataat accgtataca taatctttcg tttgtgtcca
     6421 ttcatggaag aaaacggctc ccgcaatcgt attcaaaatt aattgaaaac cagtagacaa
     6481 tggtagccct acagaaacac ccatgtactt cattccatgg aactgtccat tttgtccaac
     6541 actccaagct aagccggata aaaatccgat caagaaaatc caaggggtga ttacaggact
     6601 aacaacgaaa aagacaacta aagaaaacag aaatgcaccg atcgtcattc ctagtgtttg
     6661 ttgattggca tcgccaccaa ttttaccact tactaaacca atactacccc atgccaacat
     6721 agggactaat gctattaaaa tttccataac caatctcctt ttatttcaca tgataagaaa
     6781 cttatcagcg aaaacgttaa caacacaagc actttttttc gtttctaacg attttctaac
     6841 gtttttcatt ataaaaattt aagattggga aaaataaagt cattgttgtc ccttttcctt
     6901 cagttgattc agccgtcaaa tggataccta atttttcggc cagatttttt gctaaataaa
     6961 gtcctaatcc tgttgaatgt gttttgctaa gtcgaccatt tctcccagta aatcccttat
     7021 caaagatccg tttcaagtct tccgctggga taccgatccc tgaatcttta actgaaaggc
     7081 tgactccttg cgcttccttt tctatgctca caatgatgtt tccgtgatcc ggcgtatatt
     7141 tgatggcatt actaagcaat tggttgaaga taaaagcgat ccatttagca tcggtcaaaa
     7201 cagtatgatc ctcaccaacg atctcgtagc gaatattttt ttggataaaa taatttgcct
     7261 gactgcgtat cacaggctga atgattcctt ttagtccata ttcttggatg aggtagtccc
     7321 gcgagaaact gtcgagtctt gcataataca aaacttgttc tacgtattca tcgatttttg
     7381 ataattcatt ttctagtaat gtatactttt gatcgtcaat atcgaactcg atcgaacgaa
     7441 ctagcaattt agaagcggaa agtggcactt tgatttcatg gacccatgag tcaatataat
     7501 ctttttggtc ttgttgatta ctgattgctg attgcattac ttcttggtgt tcacggatca
     7561 gctgattgat atattcttct accagttttt cttcttctgt cctcccacca ctcaaataat
     7621 tttgcaaagg ggaagttttt tgtatgtcgg ctaattttcc ccaccatctt ctcttgctca
     7681 agtaaccacc cagcagataa aaaatcagaa agacgccctc aatcaaagct aaataagcaa
     7741 ttgtgctcaa atcaactttg atgtttggcg ccagccacat cacaaagatc gtcaatgcaa
     7801 taaaaaacag ccatcccaag atcaatgtcc actgatcttt caaatattta aaaaaattca
     7861 tcttaaccgc tcatcctttc agggaacgat atatccctgt cctacttttg tttcgatata
     7921 tccttggata cctgcctgtt cgatttttcg gcgtaaccga ttgatgttga ctgtcaatgt
     7981 attgtcatct acaaatcgtt cgtcatccca cagtgctcgc agcaattttt cccgacttaa
     8041 gatctttcca ttgtgcttca tcaaaataaa taatagacga tattcatttt tgctcaaatc
     8101 aatgatctct ccgttgattt ccatacttcc gttgtcaaca ttcaacgtaa tgccattgtg
     8161 gctcatcatt tcactagata attctgtgta gttgtaagaa cggcgaagta atgcattgat
     8221 ttttgcaatc agtacatcga tttggaaatg ttttgtcaca aaatcatctg cccccatgtt
     8281 catagccatg atcatatcca tattcgtatt cctgctggag ataaaaatga tcggtacttt
     8341 cgatacttca cgtatttttt gacaccaata atagccatcc aaaacgggaa gattaatatc
     8401 tagtaataca agctgtggtt cttctctttc aaagtctgag aacacttgat tgaaatcggt
     8461 cgttccaaag gtttcaaatt gccatttctc aagttcctcc atgatcaatt gtcgaatgat
     8521 ctcttcgtct tcgactacca tgatttttgc cataaaaaat ccctccatct ttcttcttgc
     8581 tactacaata tcaaagaagc ccttgtgaaa caagttggct tacctggaaa atgtcatttt
     8641 agcaaacgtt aaaaaaaact ggaaatatgc gatttaatcg cgtttgtctc ttccaagcag
     8701 cttaaattta ttatgttatt cttaataatt ggtctattat ctgttatttt tctttcgttg
     8761 tttttgtctt ttaaattttc gataacacca ataaccgaaa agcgttagca ctagcaatat
     8821 acctagaagg agtataagta atcgataaag atggtatttt tttgtctgtt ctatctttga
     8881 agagacttct tctgtaaagg gaactcgtct tcctgtcact agtaatcggt gagtgttcac
     8941 ggtgtaaggt gtacaagtga taagtgtgac tagatcctgc ttttctctaa ttgctaatgt
     9001 atttgttttt gttggaagga caacaatttt ctcaacgact tcataagcga gcatccgacc
     9061 atacacttca agatagaatc ggtcatcttg ctccatttct tcaagatccg taaagagttt
     9121 tttctcagca ataccgctat gggcggtaat cacggaatgt gtactctctc ctccagttgg
     9181 aaaagaggtg ccttgcagga ctgtcgctcc tctatctaac aaaagatcat tcgtttcatc
     9241 aaataaaggc aagctaacat tgattttcgg aatatagatg gctccgatca tatgttgttc
     9301 gtaatattct tttcctggat tttggacatt tctaaccgct tgctcaaaag gatctttgac
     9361 ttgtcccatt cctgggatta ctgatgtatg agcaagcgtc tgatttttct tttcatgcac
     9421 tgctaattgt tgctcatgtt tttcttcttt atcgcgttca agctgctttt gataattctc
     9481 gatccttctt tgatccaaat aattgctgag agcatctgct aaaaatggat atgtaaaaac
     9541 tgcagctccg gcaagaaaca tcactgccat aaataattgt aataaaagac tgatttgggt
     9601 ttgaggtcta ttacctattt tctttttcat tttcttcacc ttcttattta aaaaaactgc
     9661 agcaaccttt ataaaaccga ttgctgcagc ttatttttgt tgcttatact tcagacttca
     9721 ttttagattt acggtaccag ctgtacgctc caatcatcaa acttattccg attagtaaga
     9781 atgccaagag cccgttccca ccagtagcag gaagtgaccc gcgtttcata ttcggtactc
     9841 tttctggccc tggattttct actcctacga tctgaatttc ttcattatag cttccataag
     9901 agatgataaa ttcagtaaag gctgcctcac ctgttggaag tacataacct tctggcgcca
     9961 tggtctcttt catttgatag gttccataat ctaacccttg aacctcaaag tatccatttc
    10021 catctgcgac aaattctgtt gcttgtccgc ttgttgccca ctcaattcta tctgctcctg
    10081 attcataaac agctaaaggc gactcccctt tataaaatat tgcgtattca gaacggtctt
    10141 cattccacaa ttcgaatctt gcatctttaa gcaattcact gcttgatgca tcatattttt
    10201 caaactttct tccacctgtt gtgactttct ctggcggctc tactccttct tcataatcaa
    10261 attcatcatt tcgtccgatt tcaataaccg ctgtgttgtt atggaaatca tccgcttcta
    10321 gttctgtgga aaccttcaaa ttatacgtga tcgttaaatc tcctccacgt gcatctgcta
    10381 atctacctaa agtatctact ttggctactc cattttcttc tgtcaaaatc gtcacgacaa
    10441 acccgttgct tactacttct actatataat cttgtccttt tgttaaaatc gtctcgccgg
    10501 ctcgcacttc tatttcctga tcataataag ttagaccaac tgtaggcgta tctgtgattt
    10561 tgtaattttt gataacagcg cttccgtttt caaggacaga atcgatgaaa taaggaatag
    10621 gtgctgtgat cgtgtaagaa gccgtttttc ctctttctag atcaatataa gaaatcgttt
    10681 cttgttctcc agcttcatta ataatcgtta cttgtctaag gtcgctttcc tctacattca
    10741 tctgtttatc tgcttctctg atttcgtttt tcggatacaa atgcacatct gtattgtatg
    10801 tgtctttcca tgtagaacca tcccacacgc catccaccat atcaggcatc atcactggca
    10861 tcataacaac cattggagac gcgattctgg aaatatgagc tggcgaataa gtttctaaga
    10921 aaagatagac tttatctcta gaagaaggtg cttcttccgt cacttggacg gtagagaata
    10981 ccgcttctcc agcttcattc gttgtttgtt tgtcaatact tgtcagtcga tattttaaaa
    11041 accaactttc tgtgtgccgt ttttgaatcc attcaatcgc ttcggcagaa ttaagaccgt
    11101 cttctcgttc cggtgttaaa ggattatctc gaatcaaatc gtaatatact tccgttacgt
    11161 caaacaaatc aaaatcgact cctggaatat tttcttcacc aaaatccgta gaaaccaatc
    11221 cgctattcgg tcgttcttcc ggaacagatg agaaaccttt tttgtgtaat gtaatcgtca
    11281 cttcacttgg tttttgtgcg acttcttcgg ctttgactag ttttccgttc ccgaaaatcc
    11341 ctattcccgc agtaacgagt aacagtacac tcattcccgt gacaatcatt tttttattca
    11401 tttttttctc cccgttcttt ttttattaat aaaacagcac ctaacaataa aagaattcct
    11461 agtaaagaaa tcaacgttct tgcttcattc gtttgcggga atacggacca ttttcctgat
    11521 tgacctggat tgcttggacc actcggctct ttcggtttgt ccgttgtttc ttttacggtc
    11581 tcgttataaa cacgtggtgt tgcggattca taggcagaga ctggaacttt cccggattct
    11641 aattcagcca tctcctgtcc gttaactact acatattgtg gttggccttt tgcatccacc
    11701 caaaattcag ggatgatgac ctcgattctc ttacttgctt catccatctc gtaaccttcc
    11761 acacctttta attcttcaaa ataataggtg ccagaaggca agaaacgttg tcctgtcgta
    11821 acaaggccct ctttatccga aatgaatttg cttacatttt tgtcattcaa aggatcattg
    11881 tctgctggtt tgatccattg atttttcaag tcattagcag gagccatatc taaataatat
    11941 ttcccccctt ctatcatctg atatagagca aaaacagcac cttctaatgg ttttcctttt
    12001 tcagttgttc ctctttgacg tccatacttg aagaagtatg gatctcttag ataaccgaca
    12061 tttttaggat agatatgaat ctcttgcagt tcctcggttg attctgtcgg gtgataaatt
    12121 ggcaaaatag cagcaattgg gatagctgtc tgatcgatat caacatccaa tccaacttct
    12181 ggatcaatcg attcttcgaa aatcaaataa actgcatccc gatcttggtt tttcttaggt
    12241 gctttgatac gtgcaatccc gtctcctaat ccgccaaccg tatctttccc agttgttacc
    12301 gaagtcccta tttggacaag cctttcttct gtgattagct gacggatatt gtttctgttc
    12361 gtttcagcga tttgctggac gaattcttta ggagtcatgt tttcagcagc ttttttttca
    12421 tgataataat cggtcatgtc gtaaatggca aatgtcgctc catttagtgg caccgtctgt
    12481 gaaatgatat ctgctgtttc ccctgcttcc tcagagatag ccaagccatc attttcatgg
    12541 tattcaaatt gatcgttata atcgatatct cgaatcatcc gtttatgcaa cacgaattcc
    12601 acgacatctt cccctgattc ttctgccgac atcgcatgtg gaagaaggac tgctgcaagc
    12661 ggtagtaaaa caagtaattg aagcatcaaa cacaacagct ttttcattac cttgctccct
    12721 cctttttaga ttgtctgaca aacaaaccat aggctgctcc tgcaagcgcc atgaagaatg
    12781 cagcaagcag gatatagaat aatcgtccgg atcctcctgt agcaggcaac ggattttcag
    12841 gatcgttccc gacactaagc tgtaatctga ttcgatttgt ctccaaaagt tcatattcta
    12901 actcttgctg tagatcaggc gtctctgctt cttcgtttgg ataatacgaa atctcaactt
    12961 ttagtgtacc cgcttctttt cctggaacta cccgatcatt ttctatgtca ggttcttctc
    13021 gataactaat ttctaaaata aagtaacctg gaagttttct atagctgtca ggtgacttca
    13081 cttccttgat ccgatagacc ccttccaaca agtctgaagc attgaatagg gaagaatcgt
    13141 ttggatctgg tgccaagcct ccctcgatga tttcatactc tcctgcacta ttttgtttct
    13201 caattgaaaa ttctgcgcct tcaagacgtc ggctattcat actgtcaatt ttcaaaagtt
    13261 ccaaatcgat tggcgctagt tcatcttcta aggttacttg tagtacacca tcgatgattt
    13321 catagccttc cggcaaggga gcagtttcgt cttcgatgac cgtatcttca atgataaact
    13381 taccttcttc attcagtgca aactggaaca cttgatcaga tagccgatac ccatttggtg
    13441 cgacagattc cgagacctca tattcaccaa tagacagatg cagaggttgg tcgtaattta
    13501 aatcgacata cttttctact cctacggcac gagaatagaa gtatccgatc ccaggcaaac
    13561 gtttcgattc ttctggttct aacggattac cagtgtatgc tcgatacagc gggaaggaag
    13621 aatcccccac tttcctgata tcgaaacctg caccatctaa tgtatggcct tcatggtcta
    13681 attttctaac tcggatctcc cgataatcaa acgtattgta agcagtgaat gctacccata
    13741 aacgatcctc ttcatctgtt gtatacgtga gatattgcca attttcttca cctgtttgct
    13801 tgagttggat agtccctata gcattatcgt aaagtcgcaa gatccatgtg tcattttcat
    13861 caggttcttt gaaaccttct gggttctggc tctcaatgat tttgtattct gcatagttgg
    13921 tagtttcttc actatcctta taaacaaatg aatctggctc tccgattgga tgaataactt
    13981 tctcaccatc cacgattaca tatttcccgt cttcatctac tgaatagaac gtgagtctcc
    14041 ctttctcttc ctctacttcg ttcacaccag aaagcccttt cgctactgga tatccgattt
    14101 ccagtccgtt accatctgtt tcataaagtt caaactctgc gccattgatt ggcatattac
    14161 tgccttcaat tagtttattg atattcatgt ggattggctt gaattcattt cgcatttcca
    14221 gtttgacata caactcatca tactctgtgc cttctatgtt aggctcttgt acaacagaaa
    14281 gcttgtgata ttctccttca taataggaat tcggatcttt accttccggt ccgccgactc
    14341 tcgaaccaga tgtatattca tctacaagct cgtagtgacc taacatctca tcttcgttgg
    14401 ctgcatattt tttgatttgg aattgatacg tctcatctac taactgataa cgtggtggtc
    14461 cagcgatctc tctgatccaa aatatgtcgc tgactgctcc agtttcatct cctaaaatcg
    14521 gcatcttcgt ttcaaagatc gctattccgt ccgtggtagt tttacttgtt tcgattggtt
    14581 cggcttcccc actctctgga ttttggtcat ataatgcaaa ttcgactcca gccaacggct
    14641 cgccattttg acccttcttg ctgacttgga aattacggcc tttatgttcg taacgaatgg
    14701 aagaagatgg gaaatacatc cgtccagtag tgatatcatt ctcataatcc tccaggttct
    14761 gaatataggc catattgttc actgtgcaaa aatttccgtc ttgagctccc gcatcaagtt
    14821 ttacttggta cttgatcctg accatttccc cttcacctaa agaaatcgga gaaaatttat
    14881 aagcttggtt ttcccgtccg acatacgttg tcggagtcgc cactacttct ggtggggctt
    14941 gtccatgacg ttcttcatcc catatttctg gctcttggta cttcatgccg ttttctcctg
    15001 gatctagatg gtaaaactga atatctgccg atctgccagc tccaaatggt gagacattaa
    15061 aaccagtttc atcataaagt gtggcttgct ggatcgttct cttgaaggta gaagagatat
    15121 cctccaaggc tgtaacaatg tcattcttgt tgttcgcatt gtaatatcgc ggtgttacgc
    15181 catcttcttc taaatcagca atatttttca atacatttcg tccaatagca tctagtcgtt
    15241 gtctcccgct aacgtttcca tcgattccta aaccaatgga gaagatactt ggcgggttct
    15301 cttgatactt gtttttcagc caatgatgat aagcaactgt gttgatcgca gatgactttt
    15361 cttgcgaagt aggactgcca ttcgtattgt tattagaaga accatcacca acccagtgag
    15421 tcgctttatt atctccttct tttacctctc cataacgata gtgtacattt acttcatctt
    15481 ctggacggtt aaagtcagat gcataggtaa ctggatgagt aaaccctgca ttagaagtat
    15541 tcccagaaaa ctcctcaaaa tttctgaaca aaacgtcatt atcttcagca atcttatctt
    15601 ccatcacagt ccagttgctc caagctccat tagtgctcgt acgataactt gactgaatcg
    15661 gtgcataact gaaggtcggt gtcccatctc ccacaacgat taggattttt tctggatttc
    15721 tattttctcc gccattgtct ttatacaatg tttcatagcc atttttaagc cctagtgtaa
    15781 gcggagttcc tccgattggt gtacgattta atgctgtatt ctcacttccc gtcaaacgat
    15841 tgatatcatt tgtcaatgga aagatgtccg aactgatttg ctcatggtta ttgatagctg
    15901 tactattatg gtaaaaattc accataccga ttcgaatatc cagactcggg tcagagagca
    15961 gatctttact catttcgttt actgcctcaa tcaacgcagc atttttagtt tgactattgg
    16021 tcccagcggt taattcactc atactcgctg acttatctag aacaaaaacg atatcgattg
    16081 gactgatttc ctttttctcg cccccgatga catctagata gacattgtaa agggttgttg
    16141 catcttcagg atgtagatca gatgctgtgc tgcttggcag aacaggttct acccattttt
    16201 tgatgtacgc cccgttataa tccaagtaac tgttttcaaa attccgttca tttccccaaa
    16261 gctgaacggt ttgagctgga acgtttctat cttcttcgcc tgcagttgat ccattattag
    16321 tagcatagtc gtaatttcgc cagttcgctc ccccgtttgt ataatcacta tcatcgatca
    16381 agttctctcc gtcatatggc gagcctttgt ctccttgata gcgttcattg ctttgtgtcc
    16441 aatttcctaa cacttttgta tagtcattcg ttgtaaactt cgggtagcga tgctgttcgt
    16501 tttcaacttc tccgctagga tagatatatt cataggggtc ttgcgtagct gttggcccaa
    16561 gacgctccaa aataccgttg aataactggc gttgaatgcg ctggagtgct tgaccgctgg
    16621 gtacgattgg ttcgtccagc ttttccatct cattttcttc gctacgttga atttcagcgg
    16681 aatcttctgt atcagtttca ttttcttgta attcttggtt atctattctt tcatcaacca
    16741 ccacttgaag aggtagttac ccttcgttca catcggggat cttttctgtt tcaaaaatca
    16801 atgtgccggt ttctgcttct tttgaataaa cttttccgac ataccattct tgaccatcga
    16861 tcgtctcgac ttttaggtct tctccatctt catcatagga ggatagacct gttccacgaa
    16921 cgttttttac ggtaccaatc ccggcggctg ccgcatcgat tgctaaacgt attctacctt
    16981 cattggcact ttcctttttt tcaaaagcca gatgccattc aagggtttct tctttttcaa
    17041 tatacttccc ttcaattttt gcatagtcct catctaagaa tacctcatcc tcctttgttt
    17101 ctgttgactc agcagctaca tccagggtcg tcatatgata gactgactgg aaatatggtc
    17161 caaatagcaa actgacagtt actaactgca aaagcttcaa ttttaaccct ttcacatcct
    17221 tatactcctt tcaaaaatat ttgtcttctt tcacaacaat caacaaaaca atgcccatct
    17281 aattataatt taatttaatt ttaattttaa atcaaat
//
LOCUS       DAYYCW010000061        16997 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000061 DAYYCW010000000
VERSION     DAYYCW010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.062"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(507..3212)
                     /locus_tag="ACHAYA_001810"
     CDS             complement(507..3212)
                     /locus_tag="ACHAYA_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM8605605.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3518..4465)
                     /locus_tag="ACHAYA_001811"
     CDS             complement(3518..4465)
                     /locus_tag="ACHAYA_001811"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM8605606.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4655..4924
                     /gene="rpsN"
                     /locus_tag="ACHAYA_001812"
     CDS             4655..4924
                     /gene="rpsN"
                     /locus_tag="ACHAYA_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8605607.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(5100..5462)
                     /locus_tag="ACHAYA_001813"
     CDS             complement(5100..5462)
                     /locus_tag="ACHAYA_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8605608.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(5473..6594)
                     /gene="alr"
                     /locus_tag="ACHAYA_001814"
     CDS             complement(5473..6594)
                     /gene="alr"
                     /locus_tag="ACHAYA_001814"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM8605609.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6610..6960)
                     /gene="acpS"
                     /locus_tag="ACHAYA_001815"
     CDS             complement(6610..6960)
                     /gene="acpS"
                     /locus_tag="ACHAYA_001815"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM8605610.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(7090..8601)
                     /locus_tag="ACHAYA_001816"
     CDS             complement(7090..8601)
                     /locus_tag="ACHAYA_001816"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8605611.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(8923..10275)
                     /gene="murF"
                     /locus_tag="ACHAYA_001817"
     CDS             complement(8923..10275)
                     /gene="murF"
                     /locus_tag="ACHAYA_001817"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM8605612.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(10428..11504)
                     /locus_tag="ACHAYA_001818"
     CDS             complement(10428..11504)
                     /locus_tag="ACHAYA_001818"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM8605613.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            11700..13445
                     /locus_tag="ACHAYA_001819"
     CDS             11700..13445
                     /locus_tag="ACHAYA_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM8605614.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(13725..14162)
                     /locus_tag="ACHAYA_001820"
     CDS             complement(13725..14162)
                     /locus_tag="ACHAYA_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM8605615.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(14146..15519)
                     /locus_tag="ACHAYA_001821"
     CDS             complement(14146..15519)
                     /locus_tag="ACHAYA_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605616.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(15841..16329)
                     /locus_tag="ACHAYA_001822"
     CDS             complement(15841..16329)
                     /locus_tag="ACHAYA_001822"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8605617.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(16351..16953)
                     /locus_tag="ACHAYA_001823"
     CDS             complement(16351..16953)
                     /locus_tag="ACHAYA_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8605618.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         4796 a         3627 c         3081 g         5493 t
ORIGIN      
        1 aaagtcaata aactttaaga aaataattgg aaaaaaacag aaaaatatca agcgcttttt
       61 caaacgcttt aatacagcaa aaatctaaca tttttttatg ggctttttct ttgaattcta
      121 taataaaagg atagaacttg acttagaaaa tcaagttcta tcctttttga tatcttaact
      181 tttggaagag agatgtaagg ctgctttcta cccatcattc ttactactcg aagcatagcg
      241 cagttgtcac aacctcttga taaacggtgt ttaaaagctt gcatctgcgg taataagccc
      301 aaccaaatta aaaacataag aagctgtttt tatttggtcg ttcttattac ttgatgcaga
      361 gcagcttttt tacaacctct tggtacacgg tgttcaatca gctgaccctc ggtattagct
      421 caaaaaaacg gcaaaatatg aggaactatt tttccatttt tttctctaat actcaggtca
      481 aaacactgat ttcacaacct ctttttttaa tgaaaaatat ggctctcatg cgccgcttct
      541 ttgaatttcg tcattttttc ttcgatgaac ggatagattg ccgttcctac gattccaaag
      601 acaacaaaca aaccgatcat gatccagatt gctttcgtag catttggcca ataaattcct
      661 cctgcagatt cccgtaacaa gtttaccgca tgagtaaatg gcaagaacgg attgatcatt
      721 tggaagaatt tccctgagac ttggatcgga tagttcccgc ctccgccaga gatcgacagt
      781 actaagatga taatagcgat ccctttaccg acattatcga ataatgcagc caagacatag
      841 acgatcatca tgaacgctaa ggcgatcaag acagcaaatg acacgctgta gaccggattt
      901 tggacatcta ctcctagtaa gaaaatattc cctaatgtaa cgatcaagct ttgcgcaatc
      961 gccattgtca aaaatgtcag catacgagca acgaattgtt ctcgtttcgt aaaattgttt
     1021 cggtctttct ttcctaaata gtattctgtc gtagcaacac ttgaaagaag aacagcgccg
     1081 acccacagac atagtgcagt ataaaacggg gtgctagctg aaccgttatt tgcgattgga
     1141 taaagcgcat tggtttgtag ctcaacaggt tgtgtaaaga agtcactttc ttttgttgcg
     1201 tctagtttca ataatttgat gacttcgcct agatcgactt cttgttcgcc ttttcggatc
     1261 gcgtctgctg ctttttggat accagctttg atatttggcc agtcattgtt gatcaagtcc
     1321 gcagcagcgt tcacagctgt ttcgacttct ggcatttttt catttactgt tgtcaatgta
     1381 ccggtgattt cattttttag tgctggccaa tcattttgga taaaatcagc ggccaatcct
     1441 aatttttgct gaatgatcgg taattcattg ttgtataggt ctgccccttc attgatgcca
     1501 ttgacgatgg tatccatatt tccattcagc attgtattgg catcatggat ttcttgtcca
     1561 attgcaggca tttctttttg atatttttct aaaattgcca ctgcattcgt tacagttgct
     1621 ttcgttgagc tgagaagcga atcaaagtcg atctggtccg ctttgctcag tgcgccttgt
     1681 gcagtcccaa tcgtatcgat gacttggtct agtagagaag tgacagtgga actgatttga
     1741 cctgtattga taccagcaat gatgccattg atattattaa caacggcatc aatttgatct
     1801 agcaattcat caatagcctt cgtgtcttca ttttgaacag ctgtgttcaa agcagatagg
     1861 cggttttgca gattgcttag caagggtttt aattgattta gctgatcaat aattggctgt
     1921 aatttcgtat ttccagcact tgtttgcaat ttagtcagga actcgaccag tttatcgatt
     1981 gcattttgtt gttgagatat tccgtcaata aagccgttca atacattgct gatgtttttc
     2041 ttttcatcat cagtcagctt accatcttca atagctgttc ttacagtatc tattaacgaa
     2101 cctgtcgttg ttgccacttg tccaattgac ttcaatgtaa catctacact gtttgtgatg
     2161 cttggtatcg catctttcag ctgagaggca cctgattttg tttggtttgc tagatcactt
     2221 gcctgatttt ctaaattttt cacatctggc aagatatctt gtacttgttg gatgatcgtc
     2281 aagccttgtt ttgcttcatc gattccttgg ttcattgtct cttggatcga agcaaaatcg
     2341 ccgtcgatct ctgctagttg cttaccggca ttttggatct ctgggatttt ttcttgcaaa
     2401 tcaaggatga tttttgcttg atctttcaac tcaggcattt tgtcattcaa tgcaacgact
     2461 ttttcaccca tctcatctac tttagggatg taatccacga attcatttgc tttattcagt
     2521 ttttccttga tttcaggtag ttgactttgt aattcgagga cttgttttgt gtagccgtcg
     2581 atcgtgtcca aattttcatc tgttgataaa atcatatctt taactttatt gatacttact
     2641 aggttcgtat cgatgtcgta acctatctca ttgaatactt tcaataaagt cgaactggct
     2701 gttttgataa attcatttgt gatttgcgat tggatcgaac ttgctccttt atcggtgatt
     2761 ttcggtgcaa tcgcattgat tttttcattt accgtatatt cgatttttgg tttcttgata
     2821 tcaccactag taaaactcag cagatcttct gagaaatcct ttggcaagta aatacctgcg
     2881 taatactttc cggaacggac tccgtcttct aattgttctt ttgaatcaac gaattgccat
     2941 cctaattgtt tatttttatg aagcgattcg atgacttgct ccccaatggc cacttcttta
     3001 ccttgaaact ctgctggctt gtctgcactg taaacagcaa ttggcagctc tccagtattt
     3061 ccatatggat cccataatgc tttgatgtta aaccacgcat atagcgaagg gatgatcatg
     3121 atcgcgatga tcagaagtgt tgcaatcggg tttttgaaaa tccgtttcca gtcaagcaaa
     3181 tataatcgaa gcgtattttt gattgactgc atagttctcc cccttcttct tttttcagct
     3241 gaattacttt accatgtatt tcatactttt ttcaatcggc ttttttcaaa aaagtcgaat
     3301 ttttggacat atcacggcaa ttgtctcatt gtaatctctt acatattgag aaataattaa
     3361 ggattacagc ctttacttta tcatgaaata aaagggaaag ataattttat gccaaagttt
     3421 ttatctatta tacgtatacg caaaaaagct aaaacatttt ttgtctttgc ccctacaaaa
     3481 atgtttcagc ttttttcgtt ttttattcct gtatttctta tttttcgaat gacaggatga
     3541 ttttcccagc aggaggatgt ccttcaacta gctgatgtgc ttggatgaca tgcttaagtg
     3601 aagcagacag attcattgaa ataaaaacat gaaaggctcc tttttggtag gctttgatta
     3661 tgccagcaaa ggcttctgca tctagatatt cctttcttgg aacaaacgat tcataaaagc
     3721 cttccttttt ttgccgcaaa tctagatctg ggaggtcatt taatgcaaca tacctgccgc
     3781 ctggtttcat gatctggatg ccggtttctc ctttgatact tcctttcgtc gcatcgatta
     3841 caaggtctgc ctgatcagca aattgcagac ctggatttgt tttatcataa gcagcaaatg
     3901 ctgaagctcc taattttttt accttttcct catttttact ggatgcactg gttaagattc
     3961 ggattccctt ttcatggaga agctggatca gacttgaccc gaccgcacct gatgctccta
     4021 gtatcattac agtatctgtc ggctggattt ccgtcaaatg gttgattaga ttataagccg
     4081 taattcctgg cgtgaccatt ccggctgctt cttcccaact cattttatct ggaatttttg
     4141 ctacttttgc tgatggcaac acgacttctt ctccgtaagt cccgccgact gcatggacag
     4201 ctactcgatc acctacatga acagttgtga catctgaacc aatctctgtc acaataccag
     4261 cgccatcatt acctggtaca taagggaatt tcaatgttcg tatctctttc atttctccta
     4321 aacgcaaggc aacatcataa ggattcacac tgaatgcttt tatttctaca cgaacatgtc
     4381 cagaaccgac ttctctggga tgagcatcga tttcttcaaa aacatcttgt gcttctccgt
     4441 aaccgttaat tgcaaatctt cgcatggtca ccatctccct ttttctttaa gtttacgttt
     4501 atgcctttat tttgacaaaa aaaatgtctt ttcgttttgg cgcatgattc aaaacaaaat
     4561 agttgtcttt tttcgcgtaa cgtgttaaat tatttatcta aaagcgtaaa gattacgatt
     4621 tacgctaata aaaaataatt ggaggaattt gttgatggct aaaaaatcaa agattgccaa
     4681 agcaaaaaaa cagcaagaaa cgattgaacg ctatgctgct ttacgccagg aattaaaagc
     4741 gcaaaaagat tacgaaggct tgcgtaaact tcctttagat gctcgtccag aacgattgaa
     4801 aaatcgtgat ttaatcgatg gacgccctcg cggatacatg cgtaaattcg ggatgtcccg
     4861 tataaaattc cgtgaattag cacataaagg tttgattcca ggagtaaaaa aagccagctg
     4921 gtaatccaag tctggctgta atgtaaagga ggagggcgca tggaccactc cttggatcat
     4981 caaaaaagat acgttcgctt cttctagcag aaatgctggt actagaagca gacgtatctt
     5041 tttttacgca cagctggtcc ttttttatat gtaaaggctg catggttact tcgctatatt
     5101 tatttagtaa caggagtcag tgcttcgata cccacactaa ttttcaatgc tcggtcaact
     5161 tctgccataa tgggtcgatc caaatggcag accttttctt tcaatcttga tttatcgatc
     5221 gttctgatct gctccaacag gataacggag tctctttcta ttcctgttcc agtagctttg
     5281 atcccaatgt gagtcggaag cttaggttta gccatttttg ccgttatggc cgcaacaatg
     5341 atcgtcgggc taaaatggtt acctagatta ttttgaataa ccaaaactgg gcgtgttccg
     5401 ccttgctccg atcccacgac tggagaaaga tcagcaaaat aaatatctcc tctttttacc
     5461 ataggatcct ccttattctt gatattctct tggtatccgc tgtcctaatc cgcaagccac
     5521 ttcatagtgg atcgtaccta attgatctgc tatatcctgc atagtgattt ctttgtctgc
     5581 atttttgccg ataagcgtca cttttgttcc gacaggaaat tcttgaggca atcggatcat
     5641 cagttgatcc atacatacac gcccaatcgt ttcacagtag ttcccttcta ccaatagaga
     5701 aaaaccctgc atctttcgtg gccagccatc tgcataaccg atcggtatcg ttccgatcca
     5761 ctctgcttca ggagtgatat atgtttctcc atagccgatt ccttcacctg ctgggagttt
     5821 tttgacttga atcaattcag aaaccagctc aagtgctggc tgcaatggat aaacttccgg
     5881 tagtgcgtgc ccagagggat taagtccgta cattgctact ccataacgaa tcatattgcc
     5941 gattgtttca tcatgccata aagccgttgc actattgctg acatgtacat atcgaggaag
     6001 ttcgggaagt tttttcagca cttctataaa gcgttctttt tgcagattcc aatatgtatc
     6061 atctgcctga tctgctgtag aaaaatgggt aaataaacct tcccattcta atacacgact
     6121 ttctttgatg aatgcagctg cctcttttac ttcttcaggt gtacaaaatc cgatacgacc
     6181 catcccagtg tctgctttta gatgaatcga aagaggcgtc tctagcttac ttttctctaa
     6241 aacagcagct gctgcttgca accattcccg tgtacctgca gttactgata aatcatgagc
     6301 aacagctaca gtcagtgcgt cgtaaggaac catattcaag ataaggatgg gctgagtaaa
     6361 tccagcttct ctcaactcga ctccttcatc taaatttgaa acacaaaagc ccgaagctcc
     6421 acctgcaact gctgcctcag ctgtttcaat agctccatga ccgtatccgt tcgcttttac
     6481 tactgcaaac aattcttttc cttgcggaag tcttgcaact tcatttgcta cattttcagt
     6541 tatagctttt ttatgtatca ctgctttcgt tgggcggtgc caagcgacaa ccatcgtttt
     6601 tccttctttc tagtcttcca aaataatttg tgcaaaagct gtttcatttg tatgagtaat
     6661 actgacccat acattgcctt catgaggtga acgcgtcaca acaggctgac cattttcatt
     6721 ttttaaaatc tcgacatctt gaaaggtaac ttttccgatc ccagttcccc aagctttaga
     6781 gaaagcctct ttgcaagcgt aacgcccgcc taaaaattct acttggcgat gaaaaggcaa
     6841 ggactggaat agtttcattt catcagaggt caagattcgt gcaatgaatt ttggtttttc
     6901 ttcgatcagc cgagtgatcc taggaagttc gactgcatcg atcccaattc cttttatcat
     6961 atgcttttct cttttcaata aatttagaac actctcattc taccaaatta taaccatttt
     7021 gccacaaata agtccacaaa taacaaaaat gaaagtttct cttaaaaaag agaaactttc
     7081 attttatgtt taatcgttat ttgaacggat tacaaatccg cgttttttat cattgtgacg
     7141 tttaccgcca tctttttggc tgttattatt tttgctataa ccgccttttg atttgtttcc
     7201 acgataatta ccgccgtcac gacgattacg gttgtttccg ccaccacgtt tgccattttt
     7261 attgtatcct tttttctgca ttggcaatgg gcgttctggt gtgatcttca ctggcactgc
     7321 atcagctgga tcttttgctg tcgtttttag caaaagtgca actaaatctt gtgcagaata
     7381 ttcttctaat aatttatctg ctgtttgcag gtatttatct agaccatttt ctgcaagttt
     7441 tgtttcgatt tgttcaactg cagcacctaa ttgacctttg aatgcttctt tttcggttgg
     7501 tggacgtaaa gtcgtcatcc gttttttcgt caagttttca atcacatgca agtagtccat
     7561 ttcatttggt gtaacgaaag tcacagacat accgcctttt ccggcacggc ctgtacgtcc
     7621 gatacggtga acgtagcttt ctggatcttg agggatatca tagttgtaca catgagtgac
     7681 acctgaaata tctaatccac gtgcagcaac gtctgtcgct acaaggatat ctaaatgacc
     7741 gctcttaaat gaacgcaata cgctcattcg tttttgttga gacaaatcgc catggattcc
     7801 ttctgcacgg taaccgcgtg cttctaatcc gcgtgccaat tcgtctacac ggcgttttgt
     7861 acgtccgaag acgattgtta attcaggtgt ttgaacatca aacaagcgag tcatgatatc
     7921 gaatttttcg tattcttttg cgcggacata gtattgatcg atcaagtcag ctgtcatttc
     7981 tttcgctttg atcttcacat gatgtggatt tttcatgaat ttaacgccaa tattcttgat
     8041 agctggcggc atcgttgctg agaaaagtaa tgtttgtctt tgatcaggaa cttgtgaaat
     8101 gattttttcg atatcttcca agaagcccat attcaacatt tcatctgctt catccaaaac
     8161 taatgtttga acggttccaa gtttcaatgt gtggcggtta atgtggtcaa gcatacgtcc
     8221 tggtgtaccc acaacgatat gtggacgatc tttcaagcca cggatctgac ggccaatatc
     8281 tgctccgccg taaacggctt gtacacgtac ttttttgtcg cgccctaaac gatataattc
     8341 ttcttgtgtt tggatagcta attcacgtgt tggtgcgata actaaacctt gtaattcgtg
     8401 acgatctgga tcgatttttt ctaacattgg taatccaaat gcagctgttt tccctgtacc
     8461 tgtctgtgct tgtccaataa cgtcttttcc agctaatgct agtggaatcg tttctgcttg
     8521 gataggcgtt gcttcttcaa aaccagcgcg ttcgactgat ttcaacaatt ctggtgataa
     8581 ttctagttct ttaaatttca aatgaatcct cctatatggt tgttaactga ggatgtagct
     8641 tttttgctac cccgtcgatt tgttcggtca aagtgtattg tttctttttg acagtggcca
     8701 cgcacgtttt tatccattct tagcccctgt ggcaccaccg aaaaatcact ttgcgaagtt
     8761 taggcattag aaaagccaaa cgggtcgcgt tcagctttat cattattatg acatctggct
     8821 tcaacaagtt gtttctatgg caaaaccagt ccaatttctc ttcatctttc ggcaataggt
     8881 tattctaaca tagtttttcg ttttctgcaa gtttacagat ttttatttca tttgctgaag
     8941 ttgttctacc acttcaaata agcccatgct gttgctgcct tttagcataa tactatctgt
     9001 tgcttcaaga ttttgtttga tagcatcaat caatttattc ttttcttttt caaaatagaa
     9061 cacaggtagt tgggggtatt tttggttcaa ttcttctttt agtgcatgca tttgttcccc
     9121 ataaaggaaa atcatggcga atttctcagg atcgatatgt tcactcattt gcgcatgcat
     9181 atttaaagac tctttcccta attccagcat atctgctaac acagcgattc tgcgtccttt
     9241 tgttggcaga tttttaaaag tatctaatac cagtcccatg gctgttggat ttgcattgta
     9301 gacatcgctc aaaatagctg caccatttcc tgccgttagc cattcggttc tgttttgagt
     9361 gatagaaaca tgggcgagcc cttggcgtat ctcaggtatg gacagtccaa agaagcgccc
     9421 gatcgtatac gcaatcaaag cattcgttac attataactt ccaggcagcg ggatcgtaaa
     9481 tgtttctcct tctactacaa aatcggtttg tgcttttcct tttgctgtca cttccgcatg
     9541 aacatcgcca tttccaatac cgaatgttgt gactgtttgt actaatgttg ccgttaaagg
     9601 tgtcagcaac ggttcatctg cgggcacaac gagtaatccg tctgatacaa gtccgtcagt
     9661 gatttccatt ttggctttag cgattccttc tcgcgaccca agattttcta tgtgtgcttc
     9721 accgatcatt gtgattgctg cagcttttgg ttgagccata agtgaaagtt ccgtgatttc
     9781 attggcgtga tccatgccca tttccaaaac gatcttttct gtatcttggg gcatgtgcaa
     9841 aatcgtgtaa ggaagaccaa tattattgtt gtaatttcct tgcgtcttat atgttttgta
     9901 tttttcagac aagacagacg cggttagatc tttcgtggtg gttttaccat tgcttcctgt
     9961 aattgcaacg acttctgcgc ctactttttc caagtaatag gttgccaatt gctgcatggc
    10021 tttcaaagga tctgctacag gaataactgg aaaatttttt ggagcttgtt caacaggcaa
    10081 gctccaaaat gaagcagatg ctccgtttgt gatcgcttgc tggataaatt catgtccatc
    10141 tcgcgtacca gcaagaggaa cgaataaact tccttcggtt atcttccggc tgtcaaattc
    10201 gacattggta atctttgtca gtgcagagca ttcgctgccc acggcttgtg caatttcttg
    10261 tattgttaga ttcattgaga tctctcctaa aaaaggttgt gaaaaaagcg tttggcctaa
    10321 acggcgggtt cgcatgactc tcacttccaa gtaaatttct ctattcgtat ttagaagcta
    10381 gaatgaacgc ggttacgcaa tcactccagc ctcttcttta tttctcttta ttcatttttt
    10441 tcaaaaaaag attgacgctg atggtatcga ttcattccta actggatcag ttcttcaatc
    10501 aaatcaccgt atttcaagcc catattttcc cataagagtg ggtacatact gaactccgta
    10561 aatcctggca tagagtttaa ttcattcagg aataattcat ttttatttgt caaaaagaaa
    10621 tcgcaccggc tcaatccgct tccacctaac atcgtgtaag ctaacttcgc gtactcttgc
    10681 gctttttgat aaacttcttc tggcacttcg gctggaatct gcatttcgat tttattattg
    10741 atatattttg cttcataatc atagaatgct acgtctttta cgacttcacc aggcaaagtc
    10801 gtccgaacgt cttcatttcc taatacagca acttcgattt cgcgcgcttc aattccttgt
    10861 tcaacgattg ctcgagaatc atactgatag gctgttgcta aagcattttg cagctcttct
    10921 cggttttctg cttttgtaat gccgacacta gaacccatat tcgccggttt gacaaacatc
    10981 ggataaagca aagaaccttc acattgatca aatacttttt taggattttc tttccattga
    11041 ttcttaagta ctggtacata aggaacttgc ggcacaccag cagcttgtaa aatatacttg
    11101 gtcatgattt tatccatggc acatgcactg gtcaatacgc ctgcgccgac ataaggcata
    11161 ttcaatgtct ctaagaagcc ttggatcgtt ccatcttccc cgtttggccc atgtaaaact
    11221 ggaaaaacga tggctccttc ttctttgatt tcgcccggat tgatcacttt tcctgtaaag
    11281 ccttcctctg tctgtccact tgggtcccat gaaagatgca agacatcttt gctggcaggt
    11341 ttttctgtta gtaatggacc tttgacccat tgtccttctt ttgtaataaa tacgagttga
    11401 acttggtaat aattataata aatggcattt aaaactgaaa atgcggaaag aatggacact
    11461 tcatgctctg cgctgcgtcc gccatatagt aaagtaatct tcaaaagatt gtcctcctgt
    11521 attctaaatc gttcagtgat ttactctcat attttagcac aaaacaaaaa taaacgaata
    11581 acttatttat aaaaaaacga gccagcttct aaaagaacga gcgtcattta ttttttgtca
    11641 gctagattat ttgtcccgtt ttttataaaa agttatgata aagaaaagga gggatgatga
    11701 tggcgcaaac aaatccacgc agccgcttgg cagaaatcat tcgagtgttc atcaattaca
    11761 atgtagtgcc aaactttgta cagcaaaaaa atcccgaaca ggtaaaaaaa gcctttgaag
    11821 aactaggccc caccttcatc aagataggac agatgctttc tgttcgagaa gatttgcttt
    11881 cttctgcctt cactcaaaca ttcaaaacat tacaagatag tgtacctagc gacacatttt
    11941 caacggtcaa aaagacgatc gaaacagaac tttctctttc attatcagat atattcgatg
    12001 atttttcaaa atcgcctttt gcctctgctt ctatggggca ggctcatcgt gccaagctga
    12061 agaacggtga cgcggtagtc gttaagatcc aacaccctaa catcgccgaa gagatccgac
    12121 ttgatcttca actatttgaa cgagcgatcc ctttgatcaa atacattcct gaaactagcg
    12181 tagttgatct aaaaggggta ctccaagaag taaaacgttc cttgatcaat gaaatggact
    12241 tcttaaaaga aagtcaaaat ggcgaacaat tctatcaaaa gaacaatggc tggaaagaga
    12301 tccgcagccc caagatttat gacgcttttt gttcgaaaaa agtgatcgtg atggaagaaa
    12361 tgtctggtaa aaacttgaat catttaatga acgccgaaaa caaaactgaa acttttatca
    12421 ctggaattca aaacaagcag ctgaaacaag aagttgcaaa gcttttagtt gagaacttta
    12481 tgaaacaggt gtttgatgat ggttttttcc atgctgatcc tcatccaggg aatctgctat
    12541 tccatgtact aacaaaagaa gaacagacac aagctagccg aaaaactgaa acagtccatg
    12601 aaaaagagtt cggttccttt gcttttcgag cctccacttc tgcagaagac cctgttgcgc
    12661 catataccat caactacatc gattttggta tgatggggca tctttctgca ggacttaggc
    12721 aaaaactcac acaagccgtt ttagcgctgt atacgaaaga tgcttatcga atcgaaaaag
    12781 cggtccttcg actttgtcag caagaaggct cgtttgatga aagtcggttc caccaagaac
    12841 tgacaagttt tcttgaacag tattatgatt ctcctattga tgaaatcaac ttgcaagaag
    12901 tattcacaca tgtcgtgacg atttgtcatc aaaataatct gcagttcgat cgtgatatca
    12961 cgcttttgct gaaagcattt gggacattag aaggggtgat ccgcgtcctc gatcctgaag
    13021 tttccctcat ggaagtcgct tcgccttttg ctcaacatta ttttctcact catctggatg
    13081 tagaagatac attgaaacaa agcggattag acttattgga agggatgaag gctgcgccta
    13141 agatccctca acagctccac catctattag aaatgtggac ttcgggacaa ggaaaagtca
    13201 atttggaatt gaaaaagcaa gacaaattat tgagcaggat cgaaagtatg atcaatcggc
    13261 ttgttttcgg tatgatttta gctgcattga tcgtagggtc ctccctgctt gtccaagcag
    13321 cacctgtaga aaacgcagag gtcgtttcct tattagggat atttacgtat gctattgccg
    13381 cattcgtcat tatttttttg gcaatcgatg cattgatcca aatgtataag aagaggaaaa
    13441 agtagaaaaa ggttgtgaca gaaagaatca gcttcaagaa ataagaaggg gttttacata
    13501 aattgtttct caaatttttg tgaaaattcg gcttattttt cgaagaggct acttctgaaa
    13561 caccgagaat aaaaaatgtg aaaaagctgt tctgcaccca gtaataagaa caatcaaaaa
    13621 gggaacatgg aacacattta tcctgtttcc ctgttccctt tttgatgtat tcgagcataa
    13681 ataaaatacc ttcgttacct actatcttct gtacttggta aaccttagtc aattgccttt
    13741 tttaacgctt gacgaaaaga catatgatct ttctccgtaa agggttttgg accctttgtc
    13801 cttatgccag cttttcttaa ctttccacta tgataacgtt gttctagaag cgtgccgata
    13861 ttcgcttcgt caaattcttt tccagagtga tggaacactc gcacaccctt cggcaataca
    13921 agtgaagcta aaccataatc ttgcgtgatc aaaaagtcat tcttcttaac cataccgacg
    13981 attttataat ccgcactatc agctccgcgg tctacgtaga cgatttggac atgattagcg
    14041 tattctttgg tcgtataatg gtccacactg gtcacgataa ccacgtcaag accataggat
    14101 tctcctaatt ttatgacatc ttcttttact ggagaaccat ctccatcaat gaatagcctc
    14161 attctttttc gccttcttcc ataaatgata aacagttact aatccgctca gtaaaagacc
    14221 aacattgatc cattttgcca agataagcag ttgtacactt tctatcggca ctgattcatg
    14281 gttcagcgtg atcaataata gatggattgg ctctcccatt tcttttgcta aaacaaacaa
    14341 aagcgtcatc aataaaaact ggtggatcac actcccttta ttttgagtag tatacttgac
    14401 aacgatccgg cgatccattg gaatagctgt atcagtttga taatattcct tatttagcca
    14461 gctgcctgtt actacgtgcc agaaactaag cagaaagaca cctatcgcaa aaccatttgg
    14521 aatcaagagg accaataaag aagaactcag tccaagcaat tgcatagcca atggagacca
    14581 tgttaaacgt ttcaaaatcc atggtccgac tatttttgct gcaatcaagc ctgctgcata
    14641 aggcaaatac ataaaaatcg aaagtttttc taatccgtac agtatgccaa cataaaaact
    14701 gctaaataaa aaaaggaaat tccctagcat tccatttaga aaagtaaaca gatttagcca
    14761 gcttggcaac ttccattcct tcttctggtg tcctaaaaca aacacgaaca agatgaacag
    14821 cagcaagaaa ccataaatag caagatcaaa aagcctttca tcgaataaca atcgggcatt
    14881 tctcaaaaga aacaataagc caaagaatat caaaaataaa acaagctctc gcgtagctac
    14941 cagatttctt ttgatgtctt tgaaatccag ttcgtaacgc ggataatgac taaccgtatg
    15001 gtaggccatg acgtaaaaaa gtgtataaat agtaaagctt gctgcaatct gagtcggtgc
    15061 aggcaactca atcgcttgat acaggagaaa tagccagata gctgcaaata agtacttttt
    15121 cccatccata ttgataaaac cttgcgtctt ttcgtgaaaa ttgaccgtgg tattagcagg
    15181 tacaagccat gctgcactca gccctagcaa aacacttgaa aaataaaata aaggaaaata
    15241 aaatacgcct aatattccaa gcaatgaacc agcaccacca atcagcagag cgatcatcag
    15301 caacgtgtaa ctatctagac caaatcgtag actgttgact aaaaatagtc cagtcatccg
    15361 aaaggcataa aacagcgtaa atggcaaaat cgtcagccaa ttatgcgagt ctgttagtcc
    15421 caaaaataaa agataaggaa taaaaataac cgtgttactt aagataaagg gcgttgtttg
    15481 tgtaaaatta cgcccaaatc gtttgagttt ttcgaacatg acaatggctc cttaaacagt
    15541 cttataacta atctgctctt cgattttagc attaaagggg aaataaacca attttacaga
    15601 atatattctt gtaaaaaaaa atacagaaag aagcataagc ttctttctgc atttttacgg
    15661 tacttgatta ctcaagcaga caagcccttc tccacaaaag tcttgcccga cacccctgca
    15721 tccggcaggt gataaagata gaaatcaatt atcctgtgcc tccaaaatca attaggagct
    15781 ctgtaaacca gagtccttct cgacccgtcg cataaattta ctttaacaca gttcctatgt
    15841 ttatgcgaga gcttttttca ttttataatc gaccatttga aatccttgac cgatatctga
    15901 aataagttct tctgtaatct caaatccttc atgcttataa acctcgattg cttgatgatt
    15961 cccctgattc actcgcaaaa agatctctct ttcctttgtt tgcgcagcaa gttcttcata
    16021 ccagcgaaaa actgaagaag tcaatccttg accacgtact ttatttagca aatataattt
    16081 acttaaataa atttttccat ttatctcttc ataagcagtg taaccgacag tcttttcttc
    16141 gctttttaat aaaaagtagg acacaccttc tgtcagttct ttttgaatct gtttttttga
    16201 ctgataagtt ttcagcatgt aatccacttg tttttgacca atgatcggca caaaaacttc
    16261 cggccatact tcttgtatga ctgccatcag ctcatccagt tcattttctg ttacttttat
    16321 tttttccata cacccacgtc cttttttaca ttataaatga ccttgcgctt gatggaataa
    16381 aaagagcatg atcatgctat acacacccac cgaccacggt ttcaagaaaa tcacaatctg
    16441 cataaattcc ctaaaagata atttcgtcaa accagctact agacagatca agtcgtctgg
    16501 cgcaaaggga agcagcaagg taacaactag tactttttta atatctttcg tttttcttgt
    16561 cagcaattca tcaaatttct gatacttttt tggcgataaa ataagctgga caaaggctac
    16621 gccatagtaa cggacaagca aaaatccgat tgcttcccca atcactaggc caatacaact
    16681 atataaaagc ccaaatccat tgccaaacat cagcatgccc gctactgagg agatccctcc
    16741 cggcaaaata ggaatgatca cttggatcac ttgcaacaga acaaatatca tcactgcata
    16801 cttgccaaat tgctggataa acgcttgcaa agacgtgctc gatcgaaaaa tcccatgatg
    16861 ataagcataa agaatcaaca caaagaaaaa aaacagacca ataatgggca tgatctgaag
    16921 acatttttgt aaaactgatc cttttttaga catctatatt ccccacatct tttttcattt
    16981 attaaaactg cctcctt
//
LOCUS       DAYYCW010000062        16897 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000062 DAYYCW010000000
VERSION     DAYYCW010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.063"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..578)
                     /locus_tag="ACHAYA_001824"
     CDS             complement(66..578)
                     /locus_tag="ACHAYA_001824"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM8605619.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(592..2889)
                     /gene="recJ"
                     /locus_tag="ACHAYA_001825"
     CDS             complement(592..2889)
                     /gene="recJ"
                     /locus_tag="ACHAYA_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM8605620.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3031..3474)
                     /locus_tag="ACHAYA_001826"
     CDS             complement(3031..3474)
                     /locus_tag="ACHAYA_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM8605621.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3475..4263)
                     /locus_tag="ACHAYA_001827"
     CDS             complement(3475..4263)
                     /locus_tag="ACHAYA_001827"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8605622.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4260..5201)
                     /gene="rnz"
                     /locus_tag="ACHAYA_001828"
     CDS             complement(4260..5201)
                     /gene="rnz"
                     /locus_tag="ACHAYA_001828"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM8605623.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5378..5965)
                     /locus_tag="ACHAYA_001829"
     CDS             complement(5378..5965)
                     /locus_tag="ACHAYA_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM8605624.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6186..7499)
                     /gene="obgE"
                     /locus_tag="ACHAYA_001830"
     CDS             complement(6186..7499)
                     /gene="obgE"
                     /locus_tag="ACHAYA_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM8605625.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7787..8797
                     /gene="gap"
                     /locus_tag="ACHAYA_001831"
     CDS             7787..8797
                     /gene="gap"
                     /locus_tag="ACHAYA_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8605626.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8882..9817)
                     /locus_tag="ACHAYA_001832"
     CDS             complement(8882..9817)
                     /locus_tag="ACHAYA_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8605627.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            10003..10962
                     /locus_tag="ACHAYA_001833"
     CDS             10003..10962
                     /locus_tag="ACHAYA_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM8605628.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(11048..12142)
                     /locus_tag="ACHAYA_001834"
     CDS             complement(11048..12142)
                     /locus_tag="ACHAYA_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM8605629.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            12331..13092
                     /locus_tag="ACHAYA_001835"
     CDS             12331..13092
                     /locus_tag="ACHAYA_001835"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8605630.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(13207..13647)
                     /gene="fabZ"
                     /locus_tag="ACHAYA_001836"
     CDS             complement(13207..13647)
                     /gene="fabZ"
                     /locus_tag="ACHAYA_001836"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8605631.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            13867..14622
                     /gene="fabI"
                     /locus_tag="ACHAYA_001837"
     CDS             13867..14622
                     /gene="fabI"
                     /locus_tag="ACHAYA_001837"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM8605632.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(14732..15784)
                     /locus_tag="ACHAYA_001838"
     CDS             complement(14732..15784)
                     /locus_tag="ACHAYA_001838"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM8605633.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            15910..16830
                     /locus_tag="ACHAYA_001839"
     CDS             15910..16830
                     /locus_tag="ACHAYA_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM8605634.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         4915 a         3641 c         2962 g         5379 t
ORIGIN      
        1 cttttgaggg tcgctttaaa atcgcttctt taagaataac atcaagaata gcttcttttt
       61 tctgcttaat attccattaa tgtaatgatg tcatatcctt tgattttgtc gcgtccatgg
      121 agatccatca attcgatcaa aaatgcgcag cctacgacta caccgcctaa ttgttcgatc
      181 agttcgatcg tcgctttgat tgttcctcca gtagctagca aatcgtcaca aatcagcaca
      241 cgttgacctg gtttaatggc atccttatgc aatgtcaatg tatctgttcc atattctaat
      301 ccataagtca cttcgatggt ttctcgaggt aacttccctt ttttgcggac tggggcaaaa
      361 ccgactccta actcataagc aacaggacat cctacgatga atccacgggc ttctggccca
      421 actaccatat caattcgttt ttcttttgca taatttacga tttgtttagt agcttcccga
      481 taagcgtcac catctgccat cagtggggaa atatctcgaa acacaattcc ttttgacgga
      541 tagtcgggaa tactggcaat ataatctttt aaatccactc tttgtttcct cctaggttgt
      601 taaccatttc tttatggtag gaatatcgct caaaagcaaa aattcctcaa ctttgatttt
      661 attgagccgt cgctggtata atctgctctc agtcaaagga tgactttctg ggctagcgac
      721 tttttgtaaa acaccgttct ctattgtaac aaatttcaat tcaaaaaaca cctgtatcat
      781 aaaaattaat aatttttcag ggatacgcaa atattgagct attgctttta ttttatgacg
      841 tatatcaatt cgttcttgag aatggatcaa tttaaacaat tttgcgtact gttctcttgt
      901 gcctacccca ttcaaatagg cctcatcagg tgaaatcccc atcagataaa cacgattgaa
      961 tgaataagac cgaaaaactt ctttcagatc cgctaaatca tctggacaat ccataacaac
     1021 tagagagttt ggttttcttt tttcaattat tgcctttaca gaatctagtc cttcgaaaac
     1081 aatcgcagct tcctggataa tctcaggcac gaatttcaac gtcgaagaat caaatgctaa
     1141 ataaaggcta ccttccgacg ttcggctctg ttcacggaaa cgttttgccc gccaatcaaa
     1201 tacttgcaga ccacccacag aaaaatcaga taccatcagc tgcggtttct tacgcccgtt
     1261 ccattcattg atcgaaagct ttcctaccac atctacctga ttattcagca gttcttcttc
     1321 ttgagtacca aaaccaaaag cgaccgcatc aagctgactc gatgcatcgg aaaggacaaa
     1381 tttcagatgc tgttggtttg ctccgattct tttgatattt tctgcttgga cttgtcgaaa
     1441 taaaaactga ggcaaaggat tatccgtacc aaatggcgcc aatagtttca gctcatcaat
     1501 tcgctctact gttacttcat ttggcagcaa tacttcatca attctcaact caggccctct
     1561 catcaagtca atttgatgat cgactatata ttggttcatc ttctcctgta gtatagtgac
     1621 attttcactt ggcatcgtta aaccgactgc tgcatgatgt cctccgaagt aagtaaataa
     1681 atctcgcatc tggtcaagca tttcaaaaag attcaaggct tcaatactac gtccagatcc
     1741 tttagctgat ccatcttctt ttaaagtaag gacaagcgtt ggtttacccg tttcattcat
     1801 tattttccca gcaacaatgc ccaaaacgcc ttcatgccat cctcggttta ccaatagatg
     1861 gatatgattc tccttattga ccatcgctaa tgcttcttgt gttatctgct ctacgattgc
     1921 ttttctttct tcattgatct cattcaattt ttttgcttga acggatgctt cctcttcatc
     1981 aaacgtcgca agtaacgaga cggcaggacg tggatcgccc atccgaccga ttgcatttag
     2041 tctgggagca atcgaaaaac cgatcgttgt ttcgtctgcg tccctcattt ttacgccgct
     2101 ttcttcaaat agcgcctgaa gtcctatccg ttctgaatga tgaatggcat cgattcctaa
     2161 tgaaacaagg attcgattct catcagtcaa tgaaactaaa tcggcgatcg ttcctatcgc
     2221 tactaaatcc aaaaattctg caggcggttc ttccaataat gcagaagcta ctttgaatgc
     2281 tactccgact cctgccaaat ctggaaatgg atagtgcccc gctggatgac gagggtgaac
     2341 aatcgcataa gcgtctggta aattctcagg tagttcgtga tgatctgtaa tgatcacgtc
     2401 tacaccagct tgctgagcgt atgctaccgc atcatttcct gctaccccgt tatcaacagt
     2461 gatgatcaac tggattcctt cttcgatctt ttcttggtat accgattggt ttggtccata
     2521 cccatgctca aatcgatttg gcaggaatac ttctacttct gcaccaagta attccagtgt
     2581 ttctttcatt accgttgcac tggtgatccc atctgcgtca taatcaccgt aaacgaggat
     2641 tttttctcca ctgattacag cttcttggat tcggctgatt gctttatcca tatcgtgtat
     2701 cagatacgga tcatgtaatt cttgttcctt tggatgtaaa aatcggttga tatccttttc
     2761 ctcatggtaa cctctttgcc acagtaactt accgacaaat ggagataatt tcagttcacg
     2821 gatcatttgg agaaaggatt cagaaggctc tgtctcgctt aacttccaat caaaatttgc
     2881 ttgtctcact ttctcactcc taacttagtt attatagcaa aggaaatcag caaaaaaaag
     2941 cagaaagaaa gaataaaaac agatcagatc cgctagacag atgttcagca ggacctgatc
     3001 ttttgtacga ccatttatga aatatcccta ttagtcaaaa taattgaagc tttgtttggg
     3061 cgattgagtt ttttgttctt ccggcgcttt caattgactg atttgtgcgg ttttcgcttg
     3121 taactccgcc tgataagcag cttccaactc cgcacgttcg tttctaaact cgcgttgctg
     3181 ttcttctttt gcttcttcaa ttttcttttg aagttctgtt tgatattctg acatttcctg
     3241 agtcagctgt ttgatttgct ttttttgctg ccatagtgtt gccgaagcag tcaaaaatac
     3301 aatcagtgca ccaatcagcg cagaaccgat gatcactaag atcaatggcg cagaaaagga
     3361 agtgaagcca aaacttacag gaacattttt attgttcaat acagcaaaaa gaacaataat
     3421 caaaactaaa acaaatccaa gaatcacatt gctttgcttt ttcaattcct tcacctactt
     3481 tttgttaaaa attccgctgg ctaaataatc accaatcgtt ggaaacagcg tataaaattt
     3541 tgatgcaact tctaaaataa acggttgatt gatttcccgt tttggatgtc tcatattgcg
     3601 gacgatcatt tttgccaact tggcaggttc taaaacgatt tgtccgactt tttccaaata
     3661 gcttccgctt ggatcagcct tatcgaagaa atttgtctcg attggacctg gattcactgt
     3721 cgtaacagct acgccaagtg gtttcaattc taatcgcaaa gcgttggaga agccaaggac
     3781 tgcaaatttc gtggcagaat aaaccgttga ttttgcagtt gccatttttc cagccataga
     3841 ggcgatatta atgatatgac cttgtcttct ttctgccata tcgatcgcaa atttttgtgt
     3901 caaaaccatc attcctaaga tgttgacttc aaacatatcg tatgcttttc caaggtcgaa
     3961 tgtaaggaag tcttcgaaaa tcccaaaacc cgcattgttt accagaatat cgacgcgccc
     4021 gacttcttcc atcacctttt catataattc gtccacgctt tcaggattgc tgacgtctac
     4081 cttgaaagca taagcctctt tgccgctcaa gcgtctgcac tcgtctctga caccttctac
     4141 aaatgccatt cttcgtgcgc aacttataac gattgctcct tgctttgctg cttcataaca
     4201 gatttgtgcg cccagaccgc ctgaactacc tgttactaaa acaactttat cttctaaatt
     4261 catgcctctt cctcctcgcg aaatggaatc tcaatgctat caaaatcttt cataatcgtc
     4321 gtgttttcaa acacttcttg tgcttctttt tcaagctcta aagctgcttt tcctaaatat
     4381 ctcgcactga tatgagtaag tagcagccgt tttgcctgcg cttcttttgc aacttcagca
     4441 gcttgatgag tcgtggaatg gaaatagttg tgcgccattc ttgcttcatc tttattaaat
     4501 gtgctttcat gtaccagaat atcgctattt tccgcaagca caacggaatt atgtgttttt
     4561 ctcgtatcgc ctaaaatagt cacgattcgt ccttttttat ctggacctac gaaatctttt
     4621 ccattgatcg tccgcccatc ttctagttgg atcgtttcac cttgcttcag tttgccgtac
     4681 agtggtccag ctggaatacc taattctttt agacgatcca cctgcaattc tcctttatga
     4741 tcatgttcaa ccacacgata accaaaactg tcgatcccat gatttagttt ttttgtataa
     4801 acagaaaatt gctgatcagt aaaaatcggg gctgtttcat ttagctcaat gaatttcagc
     4861 ggataactta aacgcgtttg cgaaatacct aaagagactt ttatgtattc ctctatccct
     4921 tttggcccgt aaatctctaa tggcgtatcc ccaccttgaa atgaccggct gctgatgagc
     4981 ccgggaagtc caaaaatatg atcgccgtgt aaatgtgtaa tgaaaatttt accgattttt
     5041 ctcggacgaa tagtcgttcg caaaatctgc atttgcgtgc cttcgccaca gtcgaacaac
     5101 caaacttcat tgcgctcgtc taaaagtttc aatgctatac ttgtcacatt tctatgtttt
     5161 gcaggaacac ctgcacctgt tcctaaaaat tgtaattcca taatctacct gactttcttt
     5221 aagttcctct ttaattttag ggtaaaaaga agggataatc aaccgtaagt cgagaatggg
     5281 ttcaaattaa aaataacgtt ctaaagcgat agcgttttct ttgaaaatta ctataaaaac
     5341 agcagcgaac ccgtttgaga tcctctgctg ttttttatta atttgaagat ccagaacttt
     5401 cattgtcgtt ggaagtttgc ggatgtttgt ccttccaagt cttttgtgca gcttctttcg
     5461 catgattttc agcttctttc gcatcatgca catctttttc gacatcttca taatccaagc
     5521 gagtaatttc tccgcctaat tccatcatta ccaattgatt cagtgggcga ttgtcctctt
     5581 catcagcaat caagatcagt cctgtagctc cttcaccgat tttatttcct acaaagtcga
     5641 acacggtctg ggcatctttt atttctttgg catcttgtgt cgcaccgatc attcctccgg
     5701 caaaccatcc taataaaatc cctagcggac cacctaatac accaaccaac attccgatta
     5761 atccacccgt agaagatttg ttattaccgg taaagtcgat aaaatcttct attttgaatt
     5821 gatgtgtccc attaggatca tggctgacaa ctgccatctg ttctccttta atcgaacgaa
     5881 cagcatgtaa ctttttgatt tctgaaaatg cctgatatgc ctggctttct acttgaaaat
     5941 ttaaaatgat tactcgtttg ctcattaaaa acgccccttt cataccttct tgttatttat
     6001 tttaccttta attgaaaaat aagaaaagaa aagcactctt tttttccaat aaaatagggc
     6061 tgtgacaaaa gtcgtatcac agctcctttt tccgaataaa cggcgttctt gaagctgatc
     6121 acttctgtca caacctccct taaatctgaa tctatatttt ttggtatttg tttttatttg
     6181 cttgcctatt cgacaaattc aaattcaaac tcaccgatac gtacaaggtc gccatctttc
     6241 gcaccgcgtg cccgaagagc ttcatccaca cccataccac gtaattgacg agcaaaacgc
     6301 atcactgttt catcatgatc gaaattcgtc atttggaata atttttcgat cttctcacca
     6361 gataagatcc acgttgcatc tgaatcacga tcgatttgga actcaggacc ttctggattg
     6421 aatccatagt gtacagtttc ttcttccagt tcttcttcgt acaatgggaa ttctggcgtt
     6481 acttctaaca aatctgctgt agcattcaat aacgcgtcca atccttgtcg agtcactcca
     6541 gaaattggaa atactgggat gtcatcagca aattcatctt ctttttcctt gttcagctgt
     6601 tctttgaatt tcactagatt ttccggtgca tccggcatat ccattttgtt tgccacgatg
     6661 atttgcggac gctctagcaa gcgtaagtta taagtagata attctttgtt gatggcaaga
     6721 tagtcttcat aaggatctcg tccttccatc ccactcatat cgatcacatg taaaattact
     6781 cgtgttcttt cgatatgacg aaggaattgt gtacccagcc caacaccttg cgaagctcct
     6841 tcaatcaagc ctggcaagtc tgctgcggca aaacttctgc catcacttgt agtaaccatt
     6901 cctagattag gtacgagcgt cgtgaaatga taagcgccga tttttggacg agcagatgaa
     6961 ataacggata gtaaagtcga tttcccgaca gatggaaaac ctacaagtcc aacgtctgcc
     7021 aatactttca attctagttc gatttttcgc tcttgtcctg gttcaccgtt ttcagcaatt
     7081 tccggtgcag gattccgagg agaggcaaaa cggatattcc ctcgtccacc gcgtccacct
     7141 tttgccacaa ctagtgtttg accattttca attaaatctc ctaaaagagc accagtctcc
     7201 gcatctcgga cagtggttcc ttgtggtact ttaacatagg tatcttctga accacgaccg
     7261 tgcatccctt tactcatccc attttcacct ggctgcgctt tgaaatggcg attgaaccga
     7321 aaatccatca atgtacgtaa cccttcatcg acgatcaaga tgacatcgcc gccacgacca
     7381 ccgtctccgc cagccggtcc gccgtcagga acgtattttt cgcgacgaaa tgcaaccatt
     7441 ccgtctccgc ccttgccagc tttcacatcg atcgttactt gatctaaaaa catggacata
     7501 attaagtcct cctattattc atttatttat ttgactgcct gaatttccac aataaacaaa
     7561 agtcttactt attttatcgg tgaaatctga ttttgtctat catttcagca aaaagattgt
     7621 ttaccttctt ctttttttat catttggtaa ggtttccaac cttttttctg ctaccaagta
     7681 taattttttg acaaaatatt ctttgcgtag cagaataaca gtgtattaat tgtattaatt
     7741 gaggatgccg tttcacagca cccctaataa aggaggaact tttgatatga cagtaaaagt
     7801 aggtattaac ggatttgggc gtattggccg tctggctttc cgccgcatca aagaagtctc
     7861 agatgatatt gaagtagtag caatcaacga cttaacaagt ccaactatgt tagcacaatt
     7921 attgcaattt gactctactc atggtactta tcctggcaca gttactgcaa cagaagactc
     7981 aatcgtagtg gatggcgaag caactcgtgt ttatgcagaa cccgacgcaa gcaaaatccc
     8041 ttgggcaaaa gaaaacggtg tagatatcgt actagaatgt actggttttt atacatctga
     8101 agaaaaagca aaagcgcatt tagatgctgg tgtcaaacga gtagttattt ctgccccagc
     8161 tggtgctatg aaaaccatcg tctataacgt caatgatgat acattagatg aaaacgacaa
     8221 aatcatctca gcaggttcat gtacaacaaa ctgtttagca cctatggctt atttcttaaa
     8281 taatgaattt gggatcgaag ttggtacaat gacaacagtt catgcctaca cttccacaca
     8341 aatgctctta gatggacctg taaaaggcgg taatttgcgt gctgctcgtt cagctgctga
     8401 caatactatc ccacattcta ctggtgctgc gaaagcaatc ggcttagtta tccctgagct
     8461 acaaggaaaa ttacaaggac acgcacaacg tgttccagtc gttgatggtt cattaactga
     8521 attggtttca gtattgaaaa caaaagtaac tgctgatcaa gtaaacgaag caatcaaaaa
     8581 acacacagtt gacaatcctt cattcgggta tgatgaccgt caaatcgttt ctagcgatgt
     8641 cattggaaca actcaaggtt cgatctttga cccaacgcaa acagaagtaa caactgctgg
     8701 tgatttccaa cttgttaaaa ctgttgcatg gtatgacaat gagtatggat ttacttgcca
     8761 aatgattcgc ttactagaaa aattcgctaa cttgtaattt gatagatcaa tcgataaaag
     8821 aagcctttga ctggatccag tcaaaggctt ctttcttttc atcccctact tttaaactcg
     8881 ttcaatgaac atattggctt aaaaattgtt ggactacttg ctgatatttt tcaggatctg
     8941 tcttgtatgc ttccgcatgc tcggctcctt ttacaacata cttttctttt ggtcccttcg
     9001 ttgcacgata gaccttatct aacatttcaa atggtacaaa tgtatcttcg tctccatgta
     9061 taaagagcat tgggcgctgg tttttcttca attgttccac tgcatttgct tcaccgaaaa
     9121 agtagccagc acggactttt gtcactagac tcgttacttg cataagcgga aatgctggaa
     9181 gactaaacat atcgtctagc tgataagcaa gctcatcatg aacggaagaa taaccgcaat
     9241 cttcaatgat tgctttcaca ttatctggta gtttttctcc gctagtcatc ataactgttg
     9301 ctgctcccat gctgacaccg tatagaacga tttcttgcga ttttccattg ttttctagca
     9361 ctttgtttat ccactgtaca tagtctttac gctctggcca tccaaatcca atgtaatccc
     9421 cttcgctttt tccatgtcct cttgcatcag gaacaaggac attgtaaccc atatcatgaa
     9481 acatctttgc gtaattggtc atcgtttcag cgtttcccat atagccatga gccacaagag
     9541 ctgttttttc acttttcgtt tcagcaggaa gataaatcgc tgataatttc agaccgtctt
     9601 ttgaagtgat ggtccaatcg cttcggtttt cttttgaact aaaccattta tgtgcatccg
     9661 tttctttgga tgtctgtgtt gtaccaggtg tatcttcgtc taaaaagtct ttctcagaag
     9721 gcacaacagc ataattataa aaataattcc ctgcaaacac taacgcaaca atcagagcta
     9781 aagcgacgcc taaaaggatt ttcatccaaa gtttcataaa tcatccatct catttctctt
     9841 ttttcatata accttgaatt agtataatca aagaatctct actatgcaat gttcattttt
     9901 ataacccgcg aattgtaaaa ttaagttaga aaaataaaaa ggcatttatg gtacactcaa
     9961 attgtatttc cgacgaaaga aaacaaagga gtgtaaccat aaatgaccta caaacatctt
    10021 accatagacg aactgacaat gatagaatca tattatcttc aacataataa gccggttgaa
    10081 atcgctaacc gaatgggacg tgctatacaa actatttata atgtagtcaa taagttcaag
    10141 caaggcaaga ctgctcttga ttattggcac cagtataaag aaaataagaa aaaatgtggt
    10201 agaaaagtca ttcaattacc tgctcatgaa gtagattaca ttaaagagaa agtcactctt
    10261 ggttggacgc ctgacgtcat tatcgggcga aaagaaaggt ctgtttcatg cggtatgaga
    10321 acactttatc gtttattttc taaaggaata tttgatattg acacactacc gatgaaaggt
    10381 aaaagaaaac ccaatggcca tcaggaaaaa cggggaaaac aacagtatca gcgctcaatc
    10441 catgatcgac ctgataatta tcctgatttc aattctgagt ttggtcacct tgaaggtgat
    10501 acgatcgttg gcattcatca taaaagtgcc gtcattactt tagttgaaag attatctaaa
    10561 gtcattatca cgattaaacc caacggccgt aaggcattag atattgaaac tgcccttaat
    10621 caatggtttt ctcgcttccc taaaaacttc tttaaatcta ttacgtttga ctgtggaaaa
    10681 gaattttcta actggaaagc cattagtaac caacatgata ttgatatata ttttgcggac
    10741 cctggaacac cttctcaacg cccattaaac gagaattcta acgggattct gcgtcgtaat
    10801 ggactgccga aatcaatgga ttttagaaaa gtgaatcaga catttatttc cagtgtcagc
    10861 aatcaacgta atcatattcc aagaaaatca ttgaattaca gaacaccaat cgaaatattt
    10921 ttgagctatg tacaagaagc attttattct agcttaattt gacaaatcat aacctataaa
    10981 aaaacaaaat aaaaaagcag aaataactgg ctatttctag cggattattt ctgcaataac
    11041 taattatcta ttttttattt tcaattaatt taaagcttga ccatggtttc aaaaagtcaa
    11101 ggaggaataa ttcgtcatcg gagattcttc ctacaacatt gacacgtccg tcgtttttca
    11161 tatccttcaa ggcgatttgt gtttctcctt tgtattgacc gtacccttca ttgtcaatca
    11221 gtacatcccc atgatgcata tctcgcgtat tatgaggcgg gaaatcttta tctttgtagt
    11281 atacacgagt catcgttgaa cgaaggatat attctgagcg atcccctcga taactatgaa
    11341 ggttatcaaa caagcagatt cgttcgtttt ctgtaatacc ttcttccgta tctactttca
    11401 atgttggata atccgcgtta aatgcttctg ccatttcttt caattcttct tcacttgcgt
    11461 acgcatttcc aatagaaata tcatcaatca atcctgtaag gattaaatgc ttcacttgtg
    11521 tcgcaatttc aagatctcgg tggtcttcca gcgtgcacaa gccatcttgc gtaggccaag
    11581 ggccaaactc agcagattgc gaattgacga atgccatcgt attcaaatta tattttctga
    11641 atttttctga acaaaaaaca aagtgttcgt atcctaaacc agagtatcga tgaggataaa
    11701 aattatgaga acctaataaa ttatccgtgt ttggcgaata actcatgatg ttgtcaacgt
    11761 aattcgtgcc cgaactcata ttgatctcga ttttgatacc aaaaggattg cgcgtcattt
    11821 ttgcttcttc tgctcctgtg aatccgatat ctaagcggac cccgtaggca cccatcttat
    11881 ggaaaaaaga taagtcatcg taagaaatct ccaactgttc aaataacgct ggattgatat
    11941 caaccattac ttccatgcct aggctattcg cgtaatcaac tacttcttta aattctgaaa
    12001 gaactttttc tttatcatca ttgatctgca gcaaactggt aaaaacccgt ttatacccat
    12061 acttatgagc taaatccaag tacgctttgt ccttttcaaa tgttgaccgt tctggataaa
    12121 tcgagattcc taattttccc atattaccat ttcctcctgc atcttttcgt ttttgtatcc
    12181 acttacatta tagcaaacac aaagtaaatg gtctacacct tttctttttc ttttaaaata
    12241 aatgataaat tccaataatt taagtaggta ttttccctct attttgttat cattaaagat
    12301 aacaaaatag taaattggaa aggaacagtt atgaagaaaa ataaagttat tttaagtatt
    12361 agtttagcag cagcattgct gtttgctgct ggttgttctg caaacaacac gacttcttct
    12421 agttcgtcta cagaaagttc aaaaagtaca tctactgcgg aaagcacgac tgccagtagc
    12481 gtcgatttga acgcattgga attaccacaa ttgacgaaag atgtcgccga aaatgaagat
    12541 ctggtcgaaa tggtgacgaa taaaggcaca atcaaaatca aattatttcc cgaacacgca
    12601 ccaaaagcgg ttgaaaactt tatgacacat gcaaaagacg gttactacaa cggactgact
    12661 ttccatcgcg tcatcaaaga tttcatgatc caaggtggcg atcctaaagg cgatggcact
    12721 ggaggggaaa gcatttgggg agaaggattt gaaacagaga tcaacaacca cctttataat
    12781 ataagaggtg cgttatctat ggctcgctca caagatccta acagtaacgg cagccagttc
    12841 tttatcgttc aaaatacaga tgatatgcat gatggtcttt taaaagacga ctatcctcaa
    12901 gcaatcatcg acgcttataa aaacggcggt tatccttcac tagatggcca ttatactgtc
    12961 ttcggccaag tgattgaagg aatggatgtc gtggatgcta ttgcaaatat cgaaacagat
    13021 agttcagaca aaccaaaaga agaagtaaaa atcgataaaa tcaatatact gcaagaagca
    13081 aaacaaaaat aaaaaaataa cgtctgaatc ctgcatagat gacaacgtat gctaggattc
    13141 aggcgtcttc tttttacata aaaagcaagt aagcttcctt ttgttttgct atatcaggtt
    13201 ttatagtcaa gcattttggt tgacgatgaa cgtgaactcg cactcacaga cttttttatc
    13261 atctacatat gctgcagata acgctgtacc gactgatccc ttcattttgg tgatttcaaa
    13321 atgacatttc agcacatctc ctggaacaac tttttgacgg aatttggcct tattgatccc
    13381 accgatataa gctctttttc ctagaaactc ttctgatttt aggataagta tcgaacctgc
    13441 ttgtgccagt gtttctagaa tcaacgcacc cggcatcact ggattgtcag gaaagtgacc
    13501 ttggaaaaaa tcttcattga ttgttacatt ttttgtcgca acgatctttt ttcctggttc
    13561 taaagtatct acataatcaa tataacaaat aggataacga ttaggaatca attccattac
    13621 ttcagtagca gataatacgt aagacataat taaactcctt ctttcaggca attgccattt
    13681 tcattacttt gactatcaaa gtatcatatt cctaaatatt tagcaatctt tttttaattt
    13741 aagaaaaaat agtttgacaa tcaaaacatt ttgactaata attaagaaat caagaagtta
    13801 ttttagctat tattctctat ttttttacga ctatcaaatt tttatcagaa acaaaggaga
    13861 gtcattatgt catttctaga aggaaaaaaa atcgtcatta tgggcgtcgc taacaaaaaa
    13921 agcattgctt ggggttgtgc tgaagcaatg ttgaaacaag gggcagaaat tatctatact
    13981 taccaaaatg aacgaatgaa aaaatcatta gtcaaattgg tcggcgaaga agcattcact
    14041 gtagaatgtg atgtttctag cgacgaaagc atcgaaaaag caatgaatgc catcggtgaa
    14101 tatgctgggg agatccacgg ccttgtccat gcagttgctt acgccaacaa agaagaattg
    14161 tccggaaatg tgtctgatat ttccagagac ggctatcaat tagctcaaga tatcagcagc
    14221 tactctttaa ttgctgtatc aaaatacgca caaccgtttc ttgcaccaaa cagcgggatc
    14281 gttagcatga cttatctagg ttcagaacgc gcaatcccga attataacat gatgggtatt
    14341 gcgaaagctt ctcttgaagc tgctatccgt tatttggcag ctgagttctc gcctaaagga
    14401 attcgggtca atggaatctc agctggggca atcaaaacat tagctgtcac aggtgttaaa
    14461 gactatcaaa aactgatcca gttatctgaa tctcgtacgc ctgatcagca aggtgtgacg
    14521 atcgaagaag tcggtaatgc ttgtgctttc ttaatcagcc cactttccag cggaatcatc
    14581 ggtgatatta tctacgttga caaaggtgtc catctttcat aaacttttgt tgcattcttt
    14641 aaagtaaaac atataaaaat caggaaaaaa ctttttaaag tctcttcctg attttttctt
    14701 tttacgtttt tttcaactca ttactttgaa attattccac gacttcaatc cagccttctg
    14761 gtgcttctat gtctccaaat tgaatacctg tcaattcatc atacaaccgt tttgttactg
    14821 gtcctacttc cgtttcacta taaaatacat gaagattgtt attgtgataa atcccaccga
    14881 ttggagaaat aacagccgct gtaccacatg caccagcttc tgcaaattga tctagatcat
    14941 caatgaaaat atctccttca attgcttgca gtcctagtcg ttcttcagct aactgcaaca
    15001 aggaatattt cgtgatactt ggtaagatag agggcgattg cggagtgatg aattttccgt
    15061 cttttgtaat tccaaagaaa tttgctgcac ctacttcttc gattttcgta tgtgtggctg
    15121 gatcaagata aacacaatct gagtaatcca gttcttttgc ttctgttcct ggaagtaaac
    15181 tcgctgcgta atttccgccg acttttgctg caccagtccc cttaggagca gcacgatcgt
    15241 aatcagatac aataaaattc gttggtttta gtccaccttt gaaatacgct cctactggcg
    15301 tacagaagac gacaaataaa tattctggag ctggatggac accaatgttt tcaccgaccc
    15361 caatcagtaa agggcgcaag tataatgtag cgcctgttcc atagggcggc acaaaatctt
    15421 catttgcttt taccacttgc ttgacagcat caacgaacat cttttcaggg accattggca
    15481 tcagcaatcg tctagcactt tgattcatcc gtcgactatt ttgttcaggt cgaaagagat
    15541 tgaccttgcc gtcttttcga cgataagctt ttagaccttc aaaacattgc tgaccataat
    15601 gaagcgctgg agagccttca tgaatcgtca ggtaattatc ttctgtcaat tctccttctt
    15661 cccattgtcc gtctttccat ttggcaataa aacgccaagg agttttgata taagaaaaac
    15721 ctagattgtt ccaatcgatt tgcatatact taccaccttc gttattttct ccacatttta
    15781 tcattatcca tattatttgt cattttattt tcagcttttt tccttcatta tagttatttt
    15841 tgctatttcc ttttttgaaa acttcttggt acaatacaga aagaatcaaa ttttagaaaa
    15901 gaggatttta tgttttcatt attagcaact gccggtacga cagactcttc tggcgagaca
    15961 ttgacagatc aagctgtcag caaagtaaat gcgtttcagc ggttttggga cgggattgat
    16021 tgggacgcca ttttggctgc ctttatccaa aaaagtttat atctgatttt tttgatcgtc
    16081 cttttttgga ttcttcaccg tgtgggaaaa tatctgattg ataagtcttt taatcagtat
    16141 tctaaaaaag ccattctaaa cgagagccgc ttaaagactt tacatagtct tttaaatact
    16201 atttttcatt atacccttgg tttctttttt atctatgcaa ttttatctgc tatcggtgtg
    16261 ccgatcggat cacttttggc tggtgccgga attgcaggtg tggcaatcgg tcttggtgcc
    16321 caagggttca tgagtgatgt tattaccgga ttttttatta tcatggaaca acagatggat
    16381 gttggggact acatcaagct agcaaactta acgattgaag gaaccgtcgt atctgttggt
    16441 atccgtaccc ttcagctgaa agcaacggat ggtactgtcc atttcatccc gaatcgaaac
    16501 atcaccacga tcagcaatct ctctcgtgca aatatgcaag tacttttaga tattcggatt
    16561 gttcctgaag aaggatatga caaaatctat gagatcattg atcgagtaaa tcgtacatta
    16621 gctgaaaaat accaagaaga gctccaaact gaaccttcta tctttggatt agtggatctt
    16681 ggaaacagca attttgcgat tcgtacggtc tgctacgcat tgaacggaaa acaatatgcc
    16741 ttaaaagaag agttcttgag ctcctatgtc aaagaattga cagcttctgg tttcacgatt
    16801 ccgaacagtc caattgctgt cggaaaataa tttggacaaa cgatgcaacg aatctcacta
    16861 aaaagagcgt gggacaaaag taaagaacac ttttgtc
//
LOCUS       DAYYCW010000063        16771 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000063 DAYYCW010000000
VERSION     DAYYCW010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.064"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(308..1519)
                     /locus_tag="ACHAYA_001840"
     CDS             complement(308..1519)
                     /locus_tag="ACHAYA_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8605635.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLKHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFENLLKKDIK"
     gene            complement(1638..2273)
                     /locus_tag="ACHAYA_001841"
     CDS             complement(1638..2273)
                     /locus_tag="ACHAYA_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM8605636.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAKNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2409..3062)
                     /locus_tag="ACHAYA_001842"
     CDS             complement(2409..3062)
                     /locus_tag="ACHAYA_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605637.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFFKYGIQ"
     gene            complement(3165..3371)
                     /locus_tag="ACHAYA_001843"
     CDS             complement(3165..3371)
                     /locus_tag="ACHAYA_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFM8605638.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            complement(3447..4193)
                     /locus_tag="ACHAYA_001844"
     CDS             complement(3447..4193)
                     /locus_tag="ACHAYA_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004564412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFM8605639.1"
                     /translation="MKQTTQQRLNQLMSERNLKQVDILNMSLPLQKETGIKMSKSHLS
                     QYVNGKSSPDQHKLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQT
                     KVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFERVP
                     NNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYES
                     QTIILRSINELYEDRVVSPEQIRIIGKVVF"
     gene            4353..4577
                     /locus_tag="ACHAYA_001845"
     CDS             4353..4577
                     /locus_tag="ACHAYA_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HFM8605640.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGEYFFNLEVQNV"
     gene            4601..5362
                     /locus_tag="ACHAYA_001846"
     CDS             4601..5362
                     /locus_tag="ACHAYA_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFM8605641.1"
                     /translation="MNTQKFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            5374..5631
                     /locus_tag="ACHAYA_001847"
     CDS             5374..5631
                     /locus_tag="ACHAYA_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605642.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            5723..5974
                     /locus_tag="ACHAYA_001848"
     CDS             5723..5974
                     /locus_tag="ACHAYA_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HFM8605643.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            5974..6123
                     /locus_tag="ACHAYA_001849"
     CDS             5974..6123
                     /locus_tag="ACHAYA_001849"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605644.1"
                     /translation="MFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDEAEYRAKKP
                     TEGAD"
     gene            complement(6237..6644)
                     /locus_tag="ACHAYA_001850"
     CDS             complement(6237..6644)
                     /locus_tag="ACHAYA_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605645.1"
                     /translation="MSFDNTITISIILALVALISPSITAVINNKHVESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKVLPILNKSLEKL"
     gene            6805..7029
                     /locus_tag="ACHAYA_001851"
     CDS             6805..7029
                     /locus_tag="ACHAYA_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605646.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            7039..7233
                     /locus_tag="ACHAYA_001852"
     CDS             7039..7233
                     /locus_tag="ACHAYA_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605647.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            7236..8258
                     /locus_tag="ACHAYA_001853"
     CDS             7236..8258
                     /locus_tag="ACHAYA_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM8605648.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            8221..9036
                     /locus_tag="ACHAYA_001854"
     CDS             8221..9036
                     /locus_tag="ACHAYA_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFM8605649.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            9054..9836
                     /locus_tag="ACHAYA_001855"
     CDS             9054..9836
                     /locus_tag="ACHAYA_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605650.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            9836..10684
                     /locus_tag="ACHAYA_001856"
     CDS             9836..10684
                     /locus_tag="ACHAYA_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8605651.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            10681..10923
                     /locus_tag="ACHAYA_001857"
     CDS             10681..10923
                     /locus_tag="ACHAYA_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605652.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            11066..11434
                     /locus_tag="ACHAYA_001858"
     CDS             11066..11434
                     /locus_tag="ACHAYA_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM8605653.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            11449..11610
                     /locus_tag="ACHAYA_001859"
     CDS             11449..11610
                     /locus_tag="ACHAYA_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8605654.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            11607..11933
                     /locus_tag="ACHAYA_001860"
     CDS             11607..11933
                     /locus_tag="ACHAYA_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605655.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            11930..12745
                     /locus_tag="ACHAYA_001861"
     CDS             11930..12745
                     /locus_tag="ACHAYA_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFM8605656.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            12747..12872
                     /locus_tag="ACHAYA_001862"
     CDS             12747..12872
                     /locus_tag="ACHAYA_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605657.1"
                     /translation="MGFFMAKILLFLGLVGAAYLVYALLSQTDDKEDDNNDEMKF"
     gene            12884..13195
                     /locus_tag="ACHAYA_001863"
     CDS             12884..13195
                     /locus_tag="ACHAYA_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8605658.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            13192..13437
                     /locus_tag="ACHAYA_001864"
     CDS             13192..13437
                     /locus_tag="ACHAYA_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605659.1"
                     /translation="MIKKLIQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            13434..13850
                     /locus_tag="ACHAYA_001865"
     CDS             13434..13850
                     /locus_tag="ACHAYA_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM8605660.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYENSELLEVE"
     gene            13847..14185
                     /locus_tag="ACHAYA_001866"
     CDS             13847..14185
                     /locus_tag="ACHAYA_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605661.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMYGNSESAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            14182..14412
                     /locus_tag="ACHAYA_001867"
     CDS             14182..14412
                     /locus_tag="ACHAYA_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605662.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            14419..14622
                     /locus_tag="ACHAYA_001868"
     CDS             14419..14622
                     /locus_tag="ACHAYA_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM8605663.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            14619..14915
                     /locus_tag="ACHAYA_001869"
     CDS             14619..14915
                     /locus_tag="ACHAYA_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605664.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            14992..15405
                     /locus_tag="ACHAYA_001870"
     CDS             14992..15405
                     /locus_tag="ACHAYA_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8605665.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            15418..15804
                     /locus_tag="ACHAYA_001871"
     CDS             15418..15804
                     /locus_tag="ACHAYA_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605666.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            16355..16582
                     /locus_tag="ACHAYA_001872"
     CDS             16355..16582
                     /locus_tag="ACHAYA_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605667.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMLREAT
                     PKEIDQYKLVLIGIGWFKDEHIQTNMKRWLR"
     gene            16579..>16771
                     /locus_tag="ACHAYA_001873"
     CDS             16579..>16771
                     /locus_tag="ACHAYA_001873"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605668.1"
                     /translation="MKLYYVEKANCWVVEGCLSKYATDIISTQFSELGLKVIFCGEFV
                     SVKEAVLPMTAKSRRSRGSS"
BASE COUNT         5683 a         2734 c         3495 g         4859 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaaaataa
       61 aaaaagaaca gcgtaaatgt tttcgaaatg ctttcgcacc aacatttacc tgttccgtgg
      121 gtttggcacc caatgggccg tgaggggctc gaacccgcga cccgctgatt aagagttcgt
      181 tatttaatgg tattttaaat gaagataagc gaatttacag catttttaaa tgcttatata
      241 ccgcaaatta tcccaagttt ttgtatatcc tacccgagtt tatacccgaa aatataattt
      301 tttagtgtta ttttatatct ttttttaata ggttttcaaa cgctgtagtt gctgtatctt
      361 tttctttgcg taagacatgg ccataaacat tcattgtagt attaaaatct cggtgtccca
      421 atcgtttttg aagctctttt ggagtcatat taggatcgct aagcaaatag gtagctgatg
      481 tatgtctaat gtcgtgaaaa cgaatgtgac gcaaattgtg acgtttggtg aatcgtttga
      541 acttcttata cagataactt ccacgtggca aagttccatc ttgttttgta tcaaatataa
      601 aatagtgttt taatttcttt atatttaatt tccaacgaat ctttttattc tctttgatta
      661 attcatctaa catatcaaat agataagcag gaccggttac tattttttca tcatcatcat
      721 ttttcaaacc tggcagcaac cgaatatctg attttccatc cacttctgaa atccgctgat
      781 gaaatcttat ttcttgtttg tcaaagtcaa tatctttttc ttctaaagct gcaatttctc
      841 cttgtcttgc tcctgtaata aatgctagaa caattaaagt tttaatttct atgctttcct
      901 caaatgctgc ttttagcatt gcatgaattt cttcttccga ataaggttct tcgatattct
      961 tttttacgcc tttttctttt ggtattttta cattttccac aggattttct ttaattgctc
     1021 cgtactcatt tttagccatc aaaaataagt tgttaacggc gtataacata cgaagctttg
     1081 tatttcttga taaaggttta tcagagtttc tcccaggatc ttttgtattg attctttgag
     1141 cattaacaac aattttcttt atcatgtatg gtttgatatc gattagaggg ataaaatcaa
     1201 atttatcaag aaatctattc tcgatcattc gcttatagtt atgataagac tttggctcta
     1261 aatttttttc ggcctctgaa agccatattt ttctataaaa ttcaccaaaa gttatattga
     1321 acaaatccaa cgaatcatcg gtcattcctt cgaattgctc taaccagatg ttcaattctt
     1381 tatatgccgc tcttttacta cttgctttta tagttttgct ttttcttttt gccgagccat
     1441 caggtttgta tcctaataca gctcttagtc tatatgtttc gttgtctact tgttctaagt
     1501 atcctgttac ttctgacata attgaatcag ctctctttct ctgatacaat aggtactata
     1561 aagaagccta tcgtataggt ttgtttcttt cttagaacac gctcgctttg gtcggtgggg
     1621 cgtgtttttt atactaatta gttgtgaaga gcaattgaaa ctgtctctgt tccaaagtaa
     1681 cctgtttgtg cttgcaatac attatttttt gcatttgctt gggcttcaga gatatcaaaa
     1741 actattttac cagtttgttg catatcagga tttaagcttt ccataaagaa agagttagtg
     1801 atattaccat tttcatcttg atttgccgac atagatgcag ccgaatcagc ttcgaaagtt
     1861 tttccatctg cttttaattt gaagaatgag ctatcaacag tcacagcctt atcacctgca
     1921 ttttttacag ataagtcaac tactaagaaa gtgtcttttg cgttagtagg aagtacagat
     1981 gggccaactt gttttactac ttcaacggag tttactttgt attccatttt ccctacagca
     2041 acgtcatcac cgattttata agtcttttct tcttttgttg tagtttcttt tgtagtttct
     2101 ttattagtac ttgttgatgt tgcagtagct gaatctttgt tttcaccgcc acttagagca
     2161 ccaccaatac caaaaaacaa aagtactatt aatacccaaa accatacccg tttataaaac
     2221 ggttttttaa ccttatacat tttcccatct tgacccataa ctttttttgc catttaaata
     2281 ttcctcattt cttgttataa tatatttgtg atctcagaaa tgaggtatga gtccgtgttg
     2341 cagcacgggc ttttttattt ataacttttt tagagattat aggcaaaata gtagggcata
     2401 aaaatatatt attgaattcc gtatttaaaa aatcctaatt gaacttcacc ggaagtcttt
     2461 tgctgtgttg tacgcaatgc ttcttcttca gacattccat tctgtacttt ccatgcaaca
     2521 ggcgacatcc cgtatttgtt aacaaaatca gtaagtgata aagtgtcagc gtcttgctga
     2581 gcgcttgttt gttggtcttc tggattttgt tgagatgctg cttgttgttc tttctgatct
     2641 tgactgataa tattgccagc atcatctgta gtcaatccat tttcataaag ggccacgccg
     2701 aaagcttccc actctttgtt ggaccaattt gcacgatcag ctggagttga ctgtaaagtg
     2761 cgttgtttca tctgttcata tgtttcttct tgaggtgcgg tttggattgt actctgactg
     2821 gagcttataa ctgttgggct aggttccgcc gtaacttggt tggagcttgt aactgttgaa
     2881 ctagaatctg ttttagatgt agatttgcta gtagatgaac tggtttcagt tgtttctttt
     2941 gttttactta tttttgtttc ttggttagaa gtggcatctg ttgattcagc ttttttatta
     3001 tttgaacaag ctgaaagtag cagagcagta cttaacaaca acataacgct aacttttttc
     3061 attttatact cctcttttct ttgatacaat aagttgtaaa gaagcctact ataggtttaa
     3121 catcccacgt ccacaaactt tggtcggtgg gcgtgttttt ttgtttatag actttcgaaa
     3181 actaaagtag cttggattct atcgccacca ccgaaacctt tactaccacc gtttgtagtg
     3241 gaaatagtgt gtaatcgata acctttagaa cactgtctat ttatagtgtt ttcaagttct
     3301 gataggtttt gcgacccttt tcctataaac ttttctttta atacaacttg caatacaacg
     3361 taacttggca tattcttttc cttctttcta ctatgataat aaatactaac ccctagccgg
     3421 agcgccaact acgactgggg tattttttag aatacgactt tcccgattat tctaatctgt
     3481 tccggtgaga cgactctatc ttcatataat tcgtttatag agcgtagaat aatcgtttga
     3541 ctctcataat ccttatatac ttctttacac gtcacaccgt caccatctat ttcaacgata
     3601 gcaatctcac cgtcttccac gtctggctgt tccttaacaa atacaaattg tccattcctg
     3661 ataactggtt ccattgaatc gcctataata ggaacagcaa aatcagcatt gtttggaact
     3721 ctttcaaatt cttcttcctc tacaactgta tcgccataaa ctaattcagt cgggttggcg
     3781 gcagaagctg aatttttgat tgtcggaagt ttttcctttt taggaaagtg caccacgttt
     3841 cctcgatttt gctcttcgag ttgctgttca gcaaagttgt agacttttgt ttgtcttggt
     3901 ttttctaatt gattgtagat tgtttcaatg gatggaatgt tatttttctt atcttcttta
     3961 ggaacatcgt aaccaagcaa ccatgcctca ctcacattta aagttttagc caacaaataa
     4021 agcttgtgtt ggtcgggcga tgacttacca ttgacatatt gcgatagatg actttttgac
     4081 atttttattc cagtttcttt ttgcaaaggt aaagacatat tcaaaatatc aacttgcttt
     4141 aaatttcttt cagacattaa ctgattaagt cgttgctgag tcgtttgctt catcttatac
     4201 ctcctttctg atacttatta tataccttat tgaacataag ttcaataaaa aagttaaatt
     4261 aatttgaact tttatgttga caatgtttta tgcaggagtt atactttggt tacaaagttc
     4321 aaaacatttg aactagaaag gagcgaacta atatgagttt tgattattct aagttgtcgg
     4381 gaagaattgt tgagaaatat ggaacgcaat acaatttttc aatcgcttta ggtttgtcgg
     4441 aaagaagttt gtcattaaag ttgaataacc gagtaccatg gaaaagcacc gaacttcaaa
     4501 aagccattga attgctagat attccaaaag aagaaattgg cgaatatttt tttaatttag
     4561 aagttcaaaa tgtctgaact taagaaagga attcgaaaaa atgaacacac aaaaatttga
     4621 aaacaatcta tttgaattag aagttaagac ggaaaatggt gaatcgttat ttaatgctga
     4681 aacagtagca agaagtttgg gtttcgtgga aacaaaaaac ggtaaagaat atgttcggtg
     4741 gagaactgtc aatggatatt taaaaaaata cctttcgcaa gaagttgcga aaaacgactt
     4801 tatcagtgag ccaatggttt acaaattagc ctttaaagca aacaacgtac ttgctgaaaa
     4861 attccaagac tggttagcaa cggaagtcct acctcaaatc cgaaaacacg gcatgtacgc
     4921 aacagatgaa ctacttaaca atccagactt gctgattgaa gtagcaacca aactcaaaga
     4981 agaacgcacg ttacgattga tagctgaaca acgagtaaat gaattacaac cgaaggcaga
     5041 ctattacgac cgaatcttga acaacaaagg attagttaca gttagtacga ttgctaagaa
     5101 ctacggtatg agcgctgtat cgtttaataa attgctacat gaattaggta tccagttcaa
     5161 tcaaagtggc acatggctgc tatacagtaa gttccaagac aaaggataca cgcatattga
     5221 gccatttgat tatgaagata aaaacggcaa tcggcaagta aaaacaagga tgaaatggac
     5281 gcaaaaagga cacatcttct tatatgaaac attgaagaag aataattact taccgatgat
     5341 tgaacgtgaa caaacagcat aggaggtaac actatgaacg aggaaatgag ccaaacaatc
     5401 aaggtacaaa aaatgataga tgatctaaca catgcaattg atagccaagc cgataaaatc
     5461 atcaaggaat tgcaaggaca aaaagtttgg gatgcaaaaa tgcttttcaa gactatcaaa
     5521 tttgaaatga atccaacaaa aagaaagctc gccgatgtgt tggaagaaaa gttggcttcc
     5581 gctatcaacg agcaagaatt actatttgaa accgatactt ttaacagctg atgttttata
     5641 gatgacagtt tcatccgaat ctatatcatt atagattcga ttattgcaat gtacgaatta
     5701 taaatacagg aggaaataac caatggagca actagcttta gttaacctct cagaccttaa
     5761 agcttctttg gcagaatctg agattgcaaa tgaagtgtgg gatacaaagc aagctgcgga
     5821 atatttaaaa accacaacac gcacgctgac taaagatgcc gaatctggaa agattccagc
     5881 agcgaaagtt ggcagagagt ggaggtttag tagtatcgct ttgtatcagt atctaaaagg
     5941 aggaaaaaca tgggtaaatt caacagagca ctagtgttca gcgcacccct aatcgtctat
     6001 gctttaggac tttggggaag cagacaagca ttaatcggca cgatcgttta catggtttgg
     6061 atttttatgg ggcttgatga agctgagtac agagcgaaaa agcccaccga gggggctgac
     6121 taaagtgtgg ttctgtttat taggtgttta tctcgtggcc gttttagtaa ataactacat
     6181 gagaaaacgt ggcgaatact ggtatacatc ctatgcggtt ctagtatgtc taatgcttac
     6241 aatttttcta atgatttatt caaaataggt aatactttgt caatcaaata agtttcaaga
     6301 gatttttgtt ctgcatcagt gttgccgtaa ccaaaacggc ttaagaaaat tttcataacc
     6361 tctatatctt cattttgaat atatggcagg actgcataac ctgatgcttt tacagcagat
     6421 atatttttat cagtgttact accaatacaa atacccacat tgttgagaaa tgttgagaaa
     6481 gttgtttgta ttgtttgggt ttttgaatcg tgtttttgta attcaatttc tttatccttc
     6541 atcgattcaa catgcttatt atttataacc gctgtaatcg aaggggatat aagagctact
     6601 aaagctagga taatcgaaat tgtgatcgtg ttatcgaaac tcattttttc accatccagt
     6661 ttttaaacca ttatatcaaa aaggagagaa gaaataatgc aagaattagt aattttgaaa
     6721 aataaaaaaa gacacgaacg ccagcaagca tgatcgtgtc caaaagaaaa atcttttaaa
     6781 ccattataac acaggaggaa ataaatgatc tcagttaaag ggttaggcga tgaaatattc
     6841 gaagcaatga tgagcaaagc acaacaggac atcaaagaaa aaatcatttc tgctgcagcg
     6901 caaggacaaa ctagctgcac tgttcgatcg agaggaacga caccaagttt tcttttggcg
     6961 ctagaagatg aaggcatttt gaatatcaaa cgaagagatg gcagcgtcag attgttttgg
     7021 caattttagg aggccgtgat gaaagatttt gattctttag gcgccagaca gcaaccacca
     7081 aatgaagcta gtcctgttgg agttgattgg caagaaaatc cgttatatcc aggtgatact
     7141 tgctatctaa cagaagaagg ctatgtacca gtagacgcaa ttctagagta cgtccagcaa
     7201 cattatccaa agattgaatt aggaggaatt taggaatggc aaatgattta acacaaacaa
     7261 cacaacgttc attagatgag caggtaatca gtaatcttgg acggttacaa gaacaaggtt
     7321 tagaaatgcc accaggatat agtccacaaa atgcgttaaa aagtgctttt ttcgaactca
     7381 ctaacaatac aggaggaaac ttgttgcaga tggctgcaaa caatcaagaa atgaaaacct
     7441 ccatttctaa tgcactttta gacatggtta ttcaagggtt atctccagca aaaaaacaat
     7501 gctatttcat taaatacgga aacaaggttc agctaatgcg ttcatatttt ggaacaatgg
     7561 cggtccttga tcgtgtaact ggaggggcag atattacgcc tgtagtcgtt cgacaaggtg
     7621 atgagtttga agttgcaatg gatggaccaa acatggttgt caaaaagcat gaaacaaaat
     7681 tcgagaattt agacaatgaa atcattgcag cctatgtagt catcaagtta acaaatggta
     7741 aagagaccac aacagtcatg acgaaaaagc aaatcgatca aagttgggct aagtcaaaaa
     7801 tgaaagattc tggaccgcaa aaggaatttc cagaagaaat ggctaaacgg acagtaatca
     7861 atcgggcagc taaaacattg atcaacacaa gcaacgacaa cgatttatta gttcaagctg
     7921 caaaagatac gttagaaaat gaatttgaca atgatcggaa agatataacg ccacaaacag
     7981 aaaaagtggc tactctcgaa caaaaattct tttcaaacaa aaagattact gaaccaatac
     8041 aaaaagaacc agatccgatt gtgattccag atgatatcca aaatgaagta actcgagttg
     8101 ctgatgtacc tgcccatcca gaaatagagc aagcacattt gatcgaaaat gaggatatca
     8161 atccaattca agaagaattg atagatattc cagactttgg acgcgaggaa ggtgtagaag
     8221 atgtctcaga atttgaagac gacgagtacc ctttctgatg agaattatta ctccaatgaa
     8281 gctgactggc agtacatgtc aacatcacaa tataagtcct ttttaaaatg tgaagccgca
     8341 gccttagcaa agttgaaagg cgattgggca ccgacgtcgg atcccaaagc cttactcgta
     8401 ggaaactacg tacattctta ttttgaatca aaagaagtac atgaagcgtt caaagaagaa
     8461 aataaatcca aaatgttttc tagtaggaag ccgtatggat tgttgaaaga tttccaaatt
     8521 gctgaacaaa tgattgaacg acttaagcaa gaagaagcat tcatgaacat ttatcaaggt
     8581 gagaaagaaa cgatcgtgac tggggaacta tttggaacga catggaaagg caagattgat
     8641 tgcttgaatg ttgaggatga gtattttgtt gatatcaaaa caaccaaaga catgcatgag
     8701 cggaaatgga acgaaaacta cggatcaaga gaaacgttca ttgtcaattt tggttacgta
     8761 cttcaaatgg caatctatca agagcttctt ttacagcaat acggaaagat gttcatgcca
     8821 atcatcgctg cggtatcgaa gcaaacgcct agtgaggcga agttgataac gattgatcaa
     8881 gataagatga gctacgagct aatcatgcta aaagaaaata ttgaaagaat tgtcaaagtg
     8941 aaaaatggcg aagaaaggcc gataccttgc ggtacgtgcg agtattgtcg aggtaacctt
     9001 tcaattactg gattcactag catggacgat ctttaaaagg aggtgctttg cgaatgaatc
     9061 taggatacat caagttatac cgtaaagtga ctagttcatt cgtttggacc aattccgata
     9121 tgttcaagct atggatattg tgtttaatga aggctagtca tgaagataga aaatttttat
     9181 ttaacggtca agaagtacgt ttgacaagcg gacaattcgt cacaggagcc catgcaatag
     9241 caaaagagta caacgaagga gtcccgagtg acaaagcaat tgcatggcga acgctatgga
     9301 gatggcttaa gaaatttgaa aatgaagaat tattgacaat ccaatcgaac gctagatata
     9361 gcgttataac aataataaat tggtctgact atcaatcgag tgacaagcca gtgacaagcg
     9421 acggacaatc gagtgacaag ccagtgacaa caaacaagaa tgataagaat gataagaatg
     9481 ataagaataa taatcctcgc aactctcgaa aaacacgaga gtatgcagat gacgatccaa
     9541 ataaaaaatt ggccattctt ttattaaaac tcattcgaaa gaatcgtagt ctcaaagaac
     9601 ccgatttgaa caaatgggcc aatacgattc gtttgacgat tgaagctgac aaacgaactg
     9661 gaaaagaagt tcaagacatg attgtttggg ctactaacca tgatttttgg tccacagtca
     9721 tttcatcgcc aactagctta agaaaaaatt tcgatacaat ggtgggtcaa aaaaataaaa
     9781 gaaagcaaca aaatatttct aatgatgagt taccagaaac aggtgaggat tggtaatgaa
     9841 taaaaaacta agtgcaatgg ctgcacctta cggcggatta aaaacagcag atcataattg
     9901 cccgaaatgt ggtgatccat tgtatatctg gaaaacaaaa aataaagatg gtactgatcg
     9961 atgcggtcct acatgtatca ataagatttg tggttatcga gaaatggtaa ctaaaaatca
    10021 aaaagaagct atccaaaaag cgaatgaagc aatgaaaaag gacgctatca atcgaatgct
    10081 taacagttca atgattacag atgatgccat atggaccttc gattttgatg gatacaaagt
    10141 agttgatcag gaaacagcac aaataaaagc gatggctcaa gaatgggcta aaaaaattgt
    10201 aaatggtagc acgattcatg cagtgataac cggtagaaca ggagccggca aaacccatct
    10261 aggcgctgca gtgattaaag aagtcatgag ggcatctaat tataaaattg cttgttcatt
    10321 tatcagttat cgagaacttt tagaacagtt gaaatttgca atgaatgatc cagaagcaag
    10381 aaaagccgta acaggttcgc tgatggctga gataaagaaa tcagatttcg tggtaatcga
    10441 cgatttgggt gcagaactag gaagaatgga agaaaacaac caggcaacgc cttatgatgt
    10501 tgatgttctc acatcgctca cagaagctcg tctaaacaaa gctacgatat tcacaaccaa
    10561 tttatcatcg aagcaattaa aacacgcgta cggtgagcga gtgttctctc gtgtaatgaa
    10621 tggaacgaaa gggaacatag ctgtattcaa aacaacgaca gacaaaagga ggaatccagt
    10681 ttgacctttg tagtgaaaaa aatgtgctac ttagatagcc gagggcgagg agaagctagt
    10741 gtcgagtttg ctaaacacca cacgactaaa gaagaggctg accttgttgc aagtgtttgt
    10801 ggcggcgaag tagtcgaagt tattaaaccc aaaagacgat tttcaaaacc taagacaaag
    10861 ccagtgaaaa aagagtgttg ttgtccgaaa agtaaccaag catggatgag aggtgcaaaa
    10921 tgacttgttt aaaatgcaac gatgaaacag ttatttggta taagacattg cttggatggt
    10981 caacttgtga accttgtcca atatgcaatg aaaatggacg acgttcgaaa gaacgactcg
    11041 aaagactaaa gaaggagtat agcaaatggc aacaagaagc aaatacggaa acaaaaagca
    11101 cgaagtagac ggcatcactt ttgattctat cacggaagca gagtattacg atctagcctt
    11161 gtggcaagca caggcgaatg gttggaaagt gaagctccag gaaaggtttg agctgatgcc
    11221 gaagtttgaa ctagagggca agaagtatcg caagatcgag tatattcccg acttcacatt
    11281 ttacaaaagc ggcaagctgg tcaaagtcgt agatgttaag gggatgcaga caaaagactt
    11341 taagatcaag gcaaagttgt tctgtcatca atatcaagtg ccgttgatat tagccaaaaa
    11401 atatcggaat acgttcaagg aagagcgttt ttaacgaggt ggtccatcat gacaacagaa
    11461 gaagtgattc aaatgcgaat tcgaagcctt cggcgtgaga ttgacgatct ggaacgaaca
    11521 aaggcagtga tggtcaatga aacggctaga aaggcaatcg atttgcacat agagaattta
    11581 agaagggaaa tccgtagatt ggaggaatga gcgtggataa ggaagcggct tggcgaaaat
    11641 taatgttgct gattcaagat gagaactggc aagaagatga agcagtggtt gctgaagttc
    11701 agcgtctagg aaagattgct aacggacgta tacgaaaaaa gccagacaaa agaaaacagc
    11761 gcaaagggaa aatcgtcgtt gttttacacg aaggcaaaat tttgatgcaa ggaacagcta
    11821 gtgagctgtc tgcagaaact ggatatacgc gtgggactat tcgaacgtac gcttggcgaa
    11881 atcacaccga tcgaaaaggg cacgaatata agtatttgga ggaagaaaaa tgaacgaaaa
    11941 caaattaatc aaattgggtg tagcaggagc agtaatcgta ggtattggag ttatcggagg
    12001 atttaagttc ttcgaaaaaa tcgataatgg atatgtgggt gtgcgctatt caatgaacgg
    12061 cggtatcaaa gatgaagcac tgacgcaagg tgtgaaattt gtagggattg acaaagtgat
    12121 ccaatatcca attcgcttgc aaactatcca atcaaaaaat atttcagtat ctacaagcga
    12181 cggcaaaaag acaacgattg atatcaaata tgactacaaa gttgattcaa ctaaagcagc
    12241 aaaaatgtac aaggaatttg ggaatatcac ttcggaagat atcgaaagtg gatggttaaa
    12301 atctaagctt caaaaggtcg ctcgtgaagt ttatgcgaaa tatagtctgc ttgatgtcct
    12361 ttcaggagat tcctctaaag ttgaagctga ggtattaacg aactttgcta aatcagttga
    12421 atctaaaggg tttgaagtcg aagacgtaac acttggtgtt ccagatgtcg ataaagaaac
    12481 acaaaaatca atcgatgcga tcattcgagc tggtcaagaa aatgaaaaag cgaagctaga
    12541 cgcagaaact gcaaaaactc aagctgatag tgaagcttac aagaaaacaa aagctgcaga
    12601 agcagaggca gaatctaatc gcaaagtcgc cgaatcagta acagacaatt tgattcgtta
    12661 tgaagaagct caagctcgca aaaagcatgg atgggtaaca gtaaatggag cagatactgt
    12721 agttacggat gaagcaggca aataatatgg gattctttat ggctaaaatt ctcttgttct
    12781 taggtttagt aggagcagca tatctcgtgt atgcgctcct ttcccaaact gatgacaaag
    12841 aagatgacaa caacgatgaa atgaaatttt aggaggagaa ataatggacg aactaatcac
    12901 aaaagtagag cagtgggcta aagacaaggg actggatcaa gcagatccaa aagcacagtt
    12961 tttgaaagta gctgaggaat tcggggaaat agcttcggcg atggcaagaa gtaatgatga
    13021 actatttaaa gatagcgtag gagacgttat cgtcacgctg attatccttt ccatgcaaaa
    13081 agggacaaac gtacaagagt gtttagaaat ggcatacaac gaaatcaaag gacgcacagg
    13141 gaaaatggta gatggtgtat tcgtgaagtc gagtgatttg gagggcagca aatgataaaa
    13201 aagctcattc aattcagcat ggatttatat gatatcgaat caggagcaac actatctgtg
    13261 gaatcggacc atctaatcat aaattttggt ggaaagcgcc agattatttt gtgggtagtt
    13321 gatgatgtac tgtttccaga aattgttcat gatttcgaag aatcaaaagc ggttgagttt
    13381 gaaatagtga aaaaagtaat ggaattgatt gaaaaatacg aggaggacag cgaatgatac
    13441 cgaagtttag agcgtgggat aaacgaaaga acgtaatgag agatgtagcc gtcttgcatt
    13501 ttactaaaaa cggcaaaaca aactttattg aatattggat aaatcctacc gaattgaaat
    13561 catatcacgt gcgaaacatc gacctcatgc aatccacagg aatgaaagat aagaatggtg
    13621 tggatatatt tgaaggggat atagtattag tcagcgtgcg aaatggcttc gattacttag
    13681 ataataaagt ttgtattgtc aaaaattcaa tagattattc cggattagtt tgtgccactg
    13741 ttgatgaaga cttagagtat caaattttta acacagagct gtttgaagaa tacacgtatg
    13801 aagtcatcgg aaatatatac gagaatagcg agttattgga ggtagagtga ttatcttagc
    13861 tactgactac atcgattctt taaaagatga aaatgggaat gtcccagcat tgctaaattg
    13921 tggaattaaa agcaagaata gaatcatttt gttacggata tcggctgatt tagcaaaata
    13981 tctttatctg agcaagacag cagtatatta cactgcggct acacgttatc agtacaaggg
    14041 aaaaacaatc agtcaggatt attatgatcg tttaatcagt cctgatatgt atgggaattc
    14101 agaatcagca attaagctgt ttggcgcaat agagatatat gccgatgatt ttccaaattt
    14161 atggttgaag gaggaagcgg aatgaaatac gaaataccac taagtgaagc agacgtccag
    14221 tcaattatta acggtcggga ggttaataaa aaacttcctg atggtactga attagtcatc
    14281 agacaaagtt atttgaaaga tatggcagct ccagtattaa ttgatcgttt taacgtgact
    14341 gattctgtgg tagagaacca cttgaaagaa tttcgatcaa gtataaacga cactttcaga
    14401 ttagggagtt gattgacaat gaacaccaga caccgcagag tagcaaaact aagaaaacag
    14461 gaactgaatg tactaaagac aaagtttgaa aaagaatatg gaatttcagc agaagaaaca
    14521 tataaagtgg caagtcagtg tgttgctgat gcaagtgatg ctattcgtaa gtttggaatt
    14581 tcgatattaa gtgatgatcg taaatgggag gaaaaagaat gaaactaaaa gacggatttt
    14641 acgctagcag tcacggtatc ggcggtttaa tgctagatat gccgacaaag aatcctaaaa
    14701 cacgtaagaa accaaaagtc aaagtcggtg acatggtccg ctgtgaagca gaggagttca
    14761 tttatccatt tcgtggatat gtagaacacg tctataatca ctcagcgatc attcgcattg
    14821 aaaacacgat ggaatgtgat aaatggacag ctaaaagcaa agagaattta gcagtagtga
    14881 gattggtgga tatggaacta atcaatgaca aataaaaaag ccggatcgct ccgactaaca
    14941 taataaaaca gacaagttta ttatatcaca taaaaggagc agtttgaatt gatgcaattg
    15001 ttacgagagg tagatttcaa acagacaaga tgtaatgcga gagatgtgct gaagaacttt
    15061 cggcgtttgg agcggatggc aggtcgctct ttgatagata ttaagtcgcc gattattacg
    15121 gatatgccga aggcaccgaa gcacggcaat aaggcagagg atgcattgat tcagatgatg
    15181 gatatagaag cggagagaga cgcgatttta gcggctttga tggctcttag tctgattagt
    15241 cgtcagatac tctactacag tttttgtgtg ccagacagct tctcaaacta cagaattagc
    15301 cgtgaagtgg gttattcaga aagaagtata caacggatga aatcggaagc tctaatagag
    15361 tttgcagaag catataaaca cggaagaata attgcttata aataggggga ccatataatg
    15421 tggaaagatt atgtatcgtt gaaagagttg aaaaaagatc ttgttttcaa aagaattgta
    15481 gaatggtcag agagtgaatt aattttagaa gatggaacca aaatggaagt tgtatgtagc
    15541 gaatcagatt gctgcgcatg ggccgaaggt gaattcaaaa atgtaaagct tgatgcagtg
    15601 attacagata taaagatttt tgataaaggt aaccgtctct ataatggtga cggacatacc
    15661 tcttatgctg aagtcgttgt ttatcataat cgtaatgaga tcgctaaagc agaatgcaca
    15721 gcgaatgatg ggaatggcgg ctactattat agtgtttgtg ctttaaaggt caaagacaaa
    15781 ctttgtatag tgactgacgc ataaaaaaat ggcggttttt tggcggaatg atggcggttt
    15841 ttagccattt atcagtgata ttatggtagt gtcgaaagat tagtgatagg tctgagacaa
    15901 aataataata aaaggaacat cgttttatta ttgtttcaca attaagcttc gatagacagc
    15961 aacggaaata ttaagaataa ggatgtgaat tttaactcct tctaaattgt tcttattatc
    16021 tatcatccgt tgctgtttat tgttacatac agatcgctta ggcggtcttt ttattttggc
    16081 cgaaaaccta cattttcgat ggccgattat tggaggagga atagcatgtt caaactatct
    16141 gaaatcatta agaagtccga cgttgagaaa ttagagatgt taaaaaagaa gttgaagaaa
    16201 tagcagtctc ttcgtgaggc tgttttattt tgctcacaaa aatagaccac tatcgggtaa
    16261 tagtggtcag gaattaaatg aaaaagatgt tagggttgtt agctaagtat aacatcataa
    16321 cgcttacaaa acaatgcaag aaggataaat tattatgaga acttactggt atgtgtcatt
    16381 aaacaataaa tatccgaagc caatgaaagg acagcatagg cgtgtagtca tgtctgttca
    16441 aatgaaggcg aagtattcga ttgtagaaat gctaagagaa gctacaccaa aagagattga
    16501 tcagtataag ttggttctta ttggcattgg ttggtttaag gatgaacata ttcaaacaaa
    16561 catgaagagg tggttgagat gaagttatat tatgttgaaa aagcaaattg ttgggtggtt
    16621 gaaggctgtc tgtcaaagta cgctacagat attatatcta ctcaattttc agagttggga
    16681 ttgaaggtta tcttttgtgg tgagtttgtc agtgtgaaag aagcggtatt gccaatgaca
    16741 gcaaagagca gacgctcgcg tggttcaagt t
//
LOCUS       DAYYCW010000064        16729 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000064 DAYYCW010000000
VERSION     DAYYCW010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.065"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..263)
                     /locus_tag="ACHAYA_001874"
     CDS             complement(<1..263)
                     /locus_tag="ACHAYA_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8605669.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAG"
     gene            complement(265..462)
                     /locus_tag="ACHAYA_001875"
     CDS             complement(265..462)
                     /locus_tag="ACHAYA_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8605670.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(856..2583)
                     /locus_tag="ACHAYA_001876"
     CDS             complement(856..2583)
                     /locus_tag="ACHAYA_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM8605671.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2576..3769)
                     /locus_tag="ACHAYA_001877"
     CDS             complement(2576..3769)
                     /locus_tag="ACHAYA_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8605672.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3956..4600)
                     /locus_tag="ACHAYA_001878"
     CDS             complement(3956..4600)
                     /locus_tag="ACHAYA_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8605673.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4694..4828)
                     /locus_tag="ACHAYA_001879"
     CDS             complement(4694..4828)
                     /locus_tag="ACHAYA_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM8605674.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4830..6962)
                     /gene="feoB"
                     /locus_tag="ACHAYA_001880"
     CDS             complement(4830..6962)
                     /gene="feoB"
                     /locus_tag="ACHAYA_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM8605675.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6959..7432)
                     /locus_tag="ACHAYA_001881"
     CDS             complement(6959..7432)
                     /locus_tag="ACHAYA_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM8605676.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7833..8057
                     /gene="nrdH"
                     /locus_tag="ACHAYA_001882"
     CDS             7833..8057
                     /gene="nrdH"
                     /locus_tag="ACHAYA_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM8605677.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8216..10375
                     /gene="nrdE"
                     /locus_tag="ACHAYA_001883"
     CDS             8216..10375
                     /gene="nrdE"
                     /locus_tag="ACHAYA_001883"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM8605678.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10425..11390
                     /gene="nrdF"
                     /locus_tag="ACHAYA_001884"
     CDS             10425..11390
                     /gene="nrdF"
                     /locus_tag="ACHAYA_001884"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM8605679.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11479..12618)
                     /locus_tag="ACHAYA_001885"
     CDS             complement(11479..12618)
                     /locus_tag="ACHAYA_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8605680.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12927..13640)
                     /locus_tag="ACHAYA_001886"
     CDS             complement(12927..13640)
                     /locus_tag="ACHAYA_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM8605681.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13894..14595)
                     /gene="nagB"
                     /locus_tag="ACHAYA_001887"
     CDS             complement(13894..14595)
                     /gene="nagB"
                     /locus_tag="ACHAYA_001887"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM8605682.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14829..16361
                     /locus_tag="ACHAYA_001888"
     CDS             14829..16361
                     /locus_tag="ACHAYA_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8605683.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16552..>16729)
                     /locus_tag="ACHAYA_001889"
     CDS             complement(16552..>16729)
                     /locus_tag="ACHAYA_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8605684.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5193 a         3444 c         2884 g         5208 t
ORIGIN      
        1 ccagcagttc ttccaatttc actcaaattt cttattaatt cctcttgata ttcagtgaat
       61 cttattttat tagtataagc agtaaaatat agataattca ccaatctatt tattaaagga
      121 ctgggatcta attcatattt ttgtttttct aattttaaat ataccttttg taaaacctca
      181 agaatatcag aatatttagg atctttactt atagatatat cattatataa ttcatgaaca
      241 atttgcttag catttttttt cattttagca cttccctgga attgctccac ctaaaaaacc
      301 acctatagac attcctgcaa tacaagtagt tgccttggcc caatcgaccg tacatttatt
      361 tttagtgcaa tacactccat ttccataata ttttccacta tgagtggtac ccccatagat
      421 aagttgtgtc tctttaatag acaaaatttt taaatgtttc ataatctaat cacccctaaa
      481 ttaatgtttt tagactatta ttctaacatt cattaaattt caaaaaacaa ttccttcttg
      541 aaacgtagca aaataattcc tgaataagaa tttaatcact tttaagtata taaactatgt
      601 atattcttta atattttttg agaatgcact tcgtacataa taaactttaa tcacattagt
      661 ctttcatacc tacgatcgat ctctttgact cgcacccata aattcataat agtttgtcgg
      721 cgtctttgaa cgtttcgaag atgagctacg cattctcttt ttttatagac aaaaaagata
      781 gagacgaaca tggagcttcc aaatttggat atttgatcca attttggaag ctccatttat
      841 ttcgtgtctg tctttttatt ttttccagct ttcgactaat tcttggttct ctttgatcca
      901 gtcttttgct gcttgctgtg gatcttttcc attgttgatt tccaacataa ctttttccat
      961 atctttttct gaccaatgga aattgtctaa tactttgtaa gcttctggct ggtcttcttt
     1021 caatcctttt cgaaccatcg tatggatctg ctcttcgctt cccattccgt tttcaggatc
     1081 ttctaagtat ttcagatcat atttggcaaa catccaatga ggtgtccatc ctgtaactac
     1141 aattggttcg tgttttttga tcgcttgacc tagtgcaacg gtcattgctc ctgaagaaga
     1201 tgtctctact ttccagtctt tcaagttgtc atatttttgg atcgtgtttt cagcagccgt
     1261 aactacacca gctcctggct cgattcctat gattttctta cctgcctgat cggttaactc
     1321 atcgatcgaa tttacatcca tgtaggctgg cactgctaaa ccaacttttg ctcctgtcaa
     1381 attggcacct aggtcttcga cttgatcttt atattgtgca tattgtgaac catgagtttt
     1441 aggtagccaa gctgaaacca tggcatctga ctcgccattt gaaacagatt tccacatgat
     1501 tgaattatct aatggcgtca tcgttacatc atagcccatt tgtttcaata cttcaccgac
     1561 tacatttgtt gaagcaactt ctgtatccca ttcaacataa gataagttga tacgttttgt
     1621 ttctttggca gatgaaaaac tccctatacc aatcgcacct aagatcacta ctactacagc
     1681 accaatgatc agtccttgac gttttttact tttctgtgtt gttgctcctg cagctttttt
     1741 attgtttggc tggttcaaat gctgtgtaaa acggtcaatg atgattgcta agataactaa
     1801 tgcaaccccg ttgacaaatc cattcccgac ttgtgctctt tgcaatgctg ataatacgcc
     1861 gcgtcctaag ccgggtgcac cgatcataga ggcaataacg accattgaaa gtgacagcat
     1921 tgttgtttga ttgatccctg ccatgatcgt acttttagct aaaggcaatt ctagtttgaa
     1981 tagtttttga cgacttgtgc tcccgaatga atcagaagct tctactaatt ctttaggaac
     2041 ttgtcgaatc cctaaattcg taaaacgaac ggttggtggc agagcaaaga tgactgaggc
     2101 aaatacccct ggaaccatcc cgataccgaa aaatgcaact gctggaatca aataaacgaa
     2161 tcctggcatt gtctgcataa agtcaaggat cggtttgata atactttggg ctttactgct
     2221 ttttgccatc aagatcccta atggtacccc aatgatgatc gagatcacac tagataaaag
     2281 aacaagtgtg acagtattca tcaaatcatt ccatagacct tggttataaa taaaccatag
     2341 accgattaat gtgaacaacg taagtcctgg acgtttctta gaaataaaga aagctgctac
     2401 cgtcaataac aagataaaca aaggagctgg aatcactaac agtaaatttg taatccccga
     2461 catcactgcc tgtccaatcg tttgtaaaaa tgaaaatagg ccagaaaaag ttgtcgtaaa
     2521 ccattctgtg atattttcta cccagtttgc cactggtaat tgatagttca atagattatt
     2581 catgttcact cacctcgctt gtttctgcta atgcttcaat cacactacct cggatgacga
     2641 caccttttaa ttttccatcc tctacaactg caagtggagt cggtgagtca taaataatat
     2701 tgaaaatatc acttaccacc atgtctttcc caatcaccgt cacattttca tctaggaagt
     2761 cgatcaatgg acgtttttgc ttcctagctt ctaacgcttg atctgcagtg atgatccctt
     2821 tcaggtgtct ctttctgtca acagccatca gcatgctgac ttcttcatta cgcatccttg
     2881 tcaatgcaac gtttggtcca tcagattcga tattcgtcgt caatgcaggt accatgatgt
     2941 tttgagccgt aagcactttg gaacgatcga cttcttccac aaattcacgt acataatcat
     3001 ttgctggatt ggtcaatatt tcttcacctg ttccgatctg catgatttcg ccgtctttca
     3061 tcaaggcgat tctgtcccca atccgtaaag cttcattcaa atcatgggta atgaaaataa
     3121 tcgttttttg aacattggct tgcaggtcca gtaattcgtc ttgcatttct cgccggatca
     3181 gcggatcaag agccgagaac gcttcgtcca tcagcaagat ctcaggatca tttgctaaag
     3241 ctctggctag gccaacccgt tgttgcatcc caccagataa ctgactagga tattggtctt
     3301 taaatgaaag caaactagaa ttttctaatg ctttttctgc tttttcttgt cgttcttctt
     3361 taggtacacc acggacttct aacccgtatt ctgtattttc caagatcgtt cgatgtggaa
     3421 acagaccgaa gttttggaaa accatattga ttttatgacg acggacttct cgcaattctt
     3481 ctttgcttaa cttcgataca tcttctccat caatatagat actgcccgaa gttggctcaa
     3541 tcagacgatt caatagacgg atcaatgtgg atttcccact cccggaaagc cccatgatca
     3601 cgaagatttc tccttctttt acgtcaaaat tcacgtcata cacaccaacc gttgcaccgg
     3661 ttttctttaa aatctccgtt ttcggctggt gatcattcat catatctaaa gcggcttgtg
     3721 tctttttacc aaatatcttt gttaaatgtt ttacttctac ttttaccaaa caaattctcc
     3781 ttttttatgt aaattcattt ttctttctga aaaaagtgac cacactatgt taacatcatg
     3841 tagtcacttt gtcaaaaatg aaagaaattt gcttgtccaa actgaaaaaa acttggctaa
     3901 atctttgtaa ataaaaggtt tagccaagtt aatatttttt taatatttca cacaatcatt
     3961 ctgcaagctg atgctccggg taaaaaaagt ttgtcatacg ctgtagtgta tattcatttc
     4021 caataaaatg aacgatgtcc cctgccgtaa atttagcata ggggccaggt gaaacaagca
     4081 gttcttcatt atgagaaatt gcgataactg tggcgccagt cgcctgccag atattcaact
     4141 cgctaattga acggtccaga tgcattgctt tttctgataa agataattca aacggaagta
     4201 aaggattcat cttatgtatt cgcttcgttt gattgaccag ctgatccaat aattgagaaa
     4261 aatgattcag ctcatcttgt tgcttctcca cacttgccat gatttctgct tttgtatctt
     4321 ggatggattg tacatcttga tattgcaata ggaaatcttt tgctttttcc ttcgaagcaa
     4381 caaatgcccc acttccatgc cgtacttcca tgattccaag atctaccagc acattgatag
     4441 ccttgcgggt agtctcagga gaagcattgt acgtactggc aagtgttgag cgggcatgga
     4501 tcttctcacc cactttatat cggttttcaa cgatacgttc ggctaaatca acagcaatct
     4561 gctggtagcg cgggcggacg atttttttct ttccagacat tttctttcct ctttcttttt
     4621 ttactttccc tcatcttact atgctctagc aaaataagca aaaaagggga gaatagcctc
     4681 ttgcaacgac tgatcatcgc ttgatctgtt ccttttttac tgggcaagct gtttgacagt
     4741 catcacaatt cttaccggat tttaaacgag tatagacaat tgcgcctgca cttccaaaga
     4801 ttaacacact taataagaat gtcgccatcc tattcctctc cttctaatga caatatagta
     4861 taaaactggt tcttggattt tggctgacgg aacaaaacat aaacgagtcc tatcatcagt
     4921 aaaatagcaa gatacgtccc taacgctacc tgtccaccct ccaataagtg tcccaactga
     4981 taaatcacaa aactaactaa ataagctaaa ccgcattgat agcctatggc gatccaagtc
     5041 catttcttgg cattcatttc ccgatggata gccccaattg ccgcaaaaca aggcgcacaa
     5101 agtaaattga aggctaacaa tgaataagct gctgcgggag tatatgtgtg ttgtagcact
     5161 tgccagattt ctctgccatt ttcggatact tccgcatgac caaacaaaat accaaatgtg
     5221 ccaataatat tttctttagc gaccaatcct gtaatcgctg cgactgttcc ttgccagttt
     5281 ccccaaccaa gcggctgaaa aacaactgcc aggattcgcc cgaaaaaggc taagatactt
     5341 tggtcttcag aaacaggttg agcataaaaa ttataagaag agctgaacca gatcacgata
     5401 ctagaaacaa agataatcgt tcctgctttt ttaacaaaag acttgctgcg gtcataggtt
     5461 tgtcttagga ccgctgacca ttgaggaaaa tgatatgcgg gcaattccat gataaaagga
     5521 gctgtatctc cagcaaacaa acgtgttttt ttcaagctga ttccagagaa aataattgca
     5581 gtcataccaa gaaaataggc agaaggagct acccaagatt gatgaggaaa aaaggcccct
     5641 gctactaagg caataatcgg caactttgca gaacaaggca tgaaggttgt gaccatgatc
     5701 gtcaaacgac gatcttgctc gttttcaatc gtgcgactgg ccatgacgcc tggcactcca
     5761 catcctgtag caatcagcat aggaatgaag gattttcctg aaagaccgaa tttcctgaaa
     5821 agtcgatcca tgacaaaagc gatcctcgcc atatatccgc agtcttccag aaagctcaag
     5881 cataaaaaca atataatcaa ttgcggtaga aatcctaaca ctgctcccac gcctgcgatg
     5941 atgccgttca agatcagctc ctgcatccat cccgctactt gccattcagc taacaatctg
     6001 ccaacatgtt ctggtaacca tgtaccaaac aatgtgtcat tgatccaatc tgtccccatt
     6061 gttcctactg tttgaatagc cagataatag atcagccaca tcacaaatgc gaagatcggc
     6121 aaggctaacc aacgattcgt tgcaatctga tcgattttgt cactcatcga taattggaaa
     6181 gaatcatttg acacaacaca gagtgtacat agttgggtaa taaattcata acgttcattg
     6241 accagaatcg tctcactatc gtcgtccaat agtttctctg ttaactggat cagttgctcg
     6301 atctcttttt gctgagaaac tgctatctcg taatcctctc ttaccttctg atctcgttca
     6361 aaatatttca ttgcatacca gcgtttcttg ccgctttgtt ctcctagtac atcttcaatt
     6421 tcatgtaaag ctgcttctaa acgtggatca tatgtacagg gaacaggaat agaaggccgc
     6481 ttttttgcag actcctgaat cttttcgatt agtttttcga ttccttgctt tttcaatacg
     6541 ctgattggta aaactggtac accaagacca taggaaagct tttctagatt gatttttttt
     6601 ccttcttttt tgatgacatc catcatattc agaccaatga tcataggaat gtctaaatcc
     6661 aataactgcg tagttaaata aagatttcgt tctaaattgg tagcatcaat aatgttgatg
     6721 atcagttcag gctgttcctg aaccaaataa tctcttgcaa tcacttcttc tggtgtataa
     6781 ggagacaaag aatagatgcc gggtaggtcc tgaatatgaa tgttcggatt ttttttcaac
     6841 gatccttctt ttttttctac tgttacgccc ggccaattcc ctacatattg attcgaacca
     6901 gtcaagagat tgaacaagct agttttcccg ctgtttggat ttccgactaa cgcgatattc
     6961 attctttttc ctccctttgt atcaagatta gttcggcctc tgactttcgt aacgtcagtt
     7021 cataccctcg cactgtcagt tcgattggat ctcccagagg ggctagtttt cggataacta
     7081 tttttacatt tcgcgtcaat cccatatcca ttaaccgtcg cttcactgcg ccttgtccgt
     7141 ggattgctac gacgatccca gtctctcctg cttttaattg atcaagagaa gtccagcttt
     7201 cagaggaagc agtcttttta ttgacaaaga tttgacctag tatttcccga cttactgcca
     7261 ctcgatgact ttttaccagt aaaatggcat tgttgcctga taaattgacg acgacgattt
     7321 tacttccggc aacgatcccg atattttgca aatggcgttt tgttttttct tctgtttcga
     7381 ttcgttcgac ttgataaacg aaattctgct ttgcctgcgc taaggtctgc atattgattc
     7441 ctctcttgtg aaataaatca tgtattaaaa tcattcatta tatgttctaa ttttcttacg
     7501 tgttacgcaa ataaatgaga atcattctca catagaatac tctcggtaat tgagaatgtc
     7561 aattatactt gtgatattat gagcgaatct ttagtattga tagataaaaa tgagaaaaaa
     7621 atatttcttc atagttttcc acaatttttt ctctattaaa tttgctattt tcccacacga
     7681 tacttgtttt cagctctttt ttcaaaaaca caaaacttag tattccacag tttccacaac
     7741 acaatatgta gtgtttgttg tttgactttt ttactaagag ttttatacta tctattatag
     7801 ataataagaa tgtgttagag gaggcactca ccatgaacgt aaaaatcttt tctaaaaata
     7861 attgtatgca atgtaagatg gtcaaacggt ttttaacaga aaatcaaatt gcttttgaag
     7921 aagtcaatat cgatgaacag cctgaagcag tagactggtt gaaagcacaa ggattccaaa
     7981 gtgttcctgt cattacttct gatgcagaaa cagttatcgg attccgtcct gatcagttgc
     8041 gtaaacttgc cagctaacat gactaaaaga atcaaaacgt gatacatgga tttgaagggc
     8101 ttgagacagc accagttttt gtcgcaaact tccaaatcct tttttaaagc ttaaaaacaa
     8161 tgcaatgatt tgcaatcaag catcatcaaa tacgaaatga aggttgggcg tacgtatgag
     8221 tcttaaaaat ttacaagatg tcagttattt caagctgaat aatgaaatca atcggccagt
     8281 caatggtcaa atccctttga ataaggataa agaagcttta gttgcctttt ttgaggaaaa
     8341 tgtcaaacca aatacgatga cttttccatc tattatggaa aaaatcaatt acttgatcga
     8401 tcaagaatat atagaagcag aattcattca attgtattca ccatcattta ttgaaaagct
     8461 atatcaattt ttggacgagc aacatttttc tttcaaatct tttatggctg cttacaaatt
     8521 ttattctcaa tatgcgttga aaaccaacga cggttccgct tatctggaaa cttatgaaga
     8581 tcgagtagcc ttcaatgcgc tttactttgc gaacggggat gaagaattag cacttgctct
     8641 tgcagacgaa atgatccatc aacgatatca gccagctaca ccttctttct tgaatgctgg
     8701 acgtaaacgt cggggcgaat tggtgtcttg tttcttgact caagtcactg acgatatgaa
     8761 ctctatcggg cgctcaatca actctgcttt gcaattgtcc cgtattggtg gcggcgtagg
     8821 gattacttta tcaaatcttc gtgaagccgg cgctcctatc aaaggatatg agggtgctgc
     8881 aagtggtgtc gttccagtca tgaaattatt cgaagatagt ttcagctatt ctaatcaatt
     8941 aggtcagcgt caaggagctg gcgtggttta tttgaacgtt ttccatccag acattattgc
     9001 tttcctttca gcaaaaaaag aaaatgcaga tgaaaagatt cgcgtaaaaa ctctttctct
     9061 tggcgtgatc gtacctgata aattttatga attgacacgt aaaaacgaag atatgtattt
     9121 atttagtcca tatagtgtcg aaaaagaata tggtgtccca ttctcttatg taaatattac
     9181 agctgagtat gatcatctag ttgcaaatcc aaacattcgc aaacaaaaaa tcaaagcgcg
     9241 tgatcttgaa aatgaaatct caaaattaca acaagaatct ggctatcctt acgtaatcaa
     9301 tattgataca gcgaaccgcg agaatccaat cgatggcaag atcatcatga gtaatttatg
     9361 ttcagagatt ttacaagttc aaacgccttc tgtgattaac ggcaaacagg aatatgaagt
     9421 attaggaaca gatatctcat gtaacttagg ctcgacaaat atcgtgaatt tgatgcaaag
     9481 ccctaacttt ggtcaatctg tgcgtacgat gactcgtgca ttgacattta ttacagatgc
     9541 atctgatatc gatgtcgtac caccgatcca aaacggaaat aagctgaatc atacgattgg
     9601 attaggtgcc atgggcttac acacttatct tgcaaaagaa caaatggaat atggctcaga
     9661 ggattcatta gactttacaa atatttactt catgctgttg aattattgga cacttgttga
     9721 aagcaacaat atcgctcgtg aaagaaaaca agttttccat aattttgaaa aatcagctta
     9781 tgctgacggc acttatttcg aaaaatacgt aactggagaa ttccagccaa aatcagaaaa
     9841 agtcaaagca ttgtttgaag gcatttttat cccaactgct gaagattgga atgctttgaa
     9901 acaagcagta atgaaagatg gactgtatca tcaaaatcgc ttggcagtag caccaaatgg
     9961 ctcaatttca tatatcaacg atacgagtgc aagtattcat cctatcactc gcatgatcga
    10021 agaacgccaa gagaagaaaa tcggtaaaat ttattaccca gcgccttatc tatccaatga
    10081 gacgattccg tattacactt ctgcttatga tatggacatg cgtaaagtaa tcgatgttta
    10141 tgcaacggca caacaacacg tcgatcaagg catgagtttg actttattca tgcgttctga
    10201 aatcccagaa ggcatttatg aatggaagac aacaacgaag caaacgacac gtgatttgaa
    10261 tattttaaga cattacgctt tccacaaagg aatcaaatcc atttattacg tacgtacctt
    10321 tactgatgat gcagaagaaa tcggaagcaa tcaatgtgaa agctgcgtga tctaatatca
    10381 tagatagatt cgcttattca tattttaatt aaaggagcat tctcatgtca gaaacatatt
    10441 atgaagcaat aaactggaat gaaattgaag atatcattga taaatccact tgggagaagc
    10501 tgacagagca attttggcta gatacacgta tcccactttc aaatgaccta gatgattggc
    10561 gtacattgtc tcaattggaa aaagatacag taggacatgt atttggcggt ttgacactgc
    10621 tagatactgt ccaatctgaa agtggcatgg atcagttgcg taaagatgtc cgcactcccc
    10681 atgaagaagc cgttttgaac aatattcaat tcatggaatc cgttcatgcc aaaagttatt
    10741 cttctatttt tagtacactg aatacgaaaa aagaaatcga agaaattttt gactggacaa
    10801 atacgaatcc ttatctgcaa aaaaaagctg agcgtatcaa tgaaatctac aaacacggta
    10861 caccgctaga aaagaaaatt gccagcgtct ttttagaaac attcttgttc tattcaggtt
    10921 tttatactcc tctttattat ttaggaaaca acaaattggc taacgtagca gagatcatca
    10981 aactaatcat ccgagatgaa tctgtacatg gaacctacat cggttacaaa ttccaattag
    11041 gattcaacga gttacctgaa gcagaacaag aaacgttaaa agactggatg tacaatcttc
    11101 tttatgagct ttacgaaaac gaagaacgtt atacagaaga attatacgat ccaatcggct
    11161 ggacagaaga agtcaagaca ttcctacgct ataatgccaa caaagcattg atgaacctag
    11221 ggatggatcc actctttcca gatactgcca atgatgtcaa cccaatcgta atgaacggga
    11281 tttcaacagg cacaagcaac catgacttct tctcacaagt cggaaacggc tatttgttag
    11341 gtcatgtaga agcgatgaaa gacgacgatt atttgattgg attggaataa ggatttattt
    11401 taattcaaaa atttttcgaa aaataaaaag agtttagcgt acagatattt taaatgtaca
    11461 ctgaactctt ttttatgctt attcacttgt ttcttccagc acttttggac tgtctaactt
    11521 ttccgaaaca tgttccattc ttctcaataa atagattttc cggatattta atattatcgt
    11581 cttaatcact gcatagacag gtatgcaaat cagcatgcct aatattcctg cgacacttcc
    11641 tgctccaatc aataccaaga tgatcgtcaa aggatggata ttcattgatt tcccaaataa
    11701 caacggcttg atcaaatttc catctaattg ttggatcact aaaatcgaca acgccatata
    11761 caaagcttga aatggagaga taaacaaacc gacaatgatt gctggtaccg ctccaataaa
    11821 agggccgaca tacgggataa tattcgtgat tccgcaaatg accgcaagca acaagctata
    11881 aggctgatcg aatattctca ttgccaaaaa gctcattaca ccaataatca aagcatctaa
    11941 gatcgtactg ctgatatagg cagataatgt ttcgtttaat tcttttaccg tttggcgtaa
    12001 ctctttacga atcgctttcg gaaagaagtg agacaacgca tcaataaatt tgtgcccatc
    12061 cttgaacata aatagcaaaa taaatggaac ggtaaataac aaaataaaaa attgcatcat
    12121 aaatgaaatg atttttgaga cacttgcaga taaactgact aatatgatat tgaacaaatt
    12181 actaagagat aaatttgctg atgctaattg ttcttccaaa ttgaaatttt taaactcttc
    12241 ccgctgcgcc aattcattta accatttgct tgtttcatct gcatacattg gcaagctctg
    12301 ggtcaattga attgtctgtt ccattaactg aggcactaca ttcatgacag ttaaaacaac
    12361 taaaatgatt acgaccaaaa acgtcaataa aaaaccgaac actctcggga tccgatgctt
    12421 ttcgagaaaa acaacgattg gatcgaacat ataatagata aaaccagcaa caagcaatgg
    12481 taaaaaaatt gccgaaagca ttttcaatat tggactaaaa atattcggca tttgtaaaat
    12541 aaagtacacg ccgatgatcg tgagtactag ctccactgtc cagaaaaata attttgattg
    12601 tttaaaacgt gattgcatac atctcctcca aggtcctttg atacttctat tctatcattt
    12661 aacaaagagt tacgcgctga gattttcatt tgcttaaaaa aaacaaaaaa aataaacata
    12721 ttttaatgag tagacgagta cttaaacaaa aacacggttt ggagtagtga caaaaattat
    12781 actgcagtcc ctttttccaa ataaacggtg ttcaaaagct tgcatcttcg taataagccc
    12841 agccaaacga aaaatataag gaactatttt tatttggctg ggcttattac ttgatgtagg
    12901 acatctattt tacaacctca gatcatttat tgcgcaatac tgactaaatg caactttgtg
    12961 atatttttgt gtacggacaa ctcctcgatc acatcggcat aagtgagccc ttttggaaga
    13021 tcaatcgtat agatattttt gtacactcgg tagtcatcaa cgaattttac gtcaaaattc
    13081 acatcttcga ttacgatatt tttctcttca aaataatcgg taagaaaatc tttggtttct
    13141 tttctatgga taaattggat ttctaacttt ttagtcgtag gtacatggat cacgcgtttc
    13201 actactgtga gtgcaatagc aatcccgata aaactgatga tcgcaatgga atagtaaccc
    13261 atgccgatgg cgatccctaa tccagcaatt gcccataaag aagcggcagt cgtaagtccc
    13321 gtcaccgatt gtttcgtgac gatgatcgtt cccgcgccta agaaaccgac accactcacc
    13381 acttgagcaa tcagccttgc ttcatctgaa cgaatcgttc cagcaagctg tggatactct
    13441 aaagcatctc ttaatgcacc actagcgatc tcaacttgga tcaatgcaat gatacatgct
    13501 cccatacaaa ctaaaatatg cgtacgcata cctgctggac gatttttata ttgtcgttca
    13561 aaaccgattg ctccgccaaa tagcatagcg atccctaaac gtaaaataat ctctggaatt
    13621 gtcaatacca tatcattcat ctaaacaccc tccagtccaa tatatcatgt ctgaaaggag
    13681 cagacaaaaa aagtgcaatt tttacctctt acgaaaaaag gactgtgaca aaatttttgt
    13741 catagtcctt tttatgaata aacggtgttc aatcagctga ccctcggtat tactcaacaa
    13801 acggcaaaat atggggagct atttttcctt tgtcttctct aatactcagg tcaaaacgct
    13861 gatttcacaa cctctttagc ctactattga aaattacaac ttacttgctg cttcttcatc
    13921 gatgatcaca acaacattgt cgtgattttg caatacactt gctggcaaat gattcgttac
    13981 aggtccttca atcatccctt tgattgcatc agctttgctt tcgccaaatg ccatcaagac
    14041 gatttcttta cttttcataa ttgaaccgat tcccattgaa acagcttttg ttggtacatc
    14101 ttctgcttta tcgaagaatc gtttgtttgc attgattgtt gattctgtta agtcaactac
    14161 tgaagtcgtt ccgtcaaatg aagcaccagg ttcattgaaa ccgatatgtc cattttgccc
    14221 gattcctaag atttggatat cgattggatg tgcatcgatg atttgatcat actttttgca
    14281 tgctgcttcc agatcttctg cttttccatc tggtacaaac gtttctttga atggtttttt
    14341 atcaaataat tggacatcca tgaaatgacg gtagctttgt tcatctgaac cgcctagtcc
    14401 aacgtactca tctaagttta cagaagtcat ttcactgaaa tccaaatcgc ttgaagtcat
    14461 ttctttatat aaagtaactg gtgtgcttcc tgttgccaat cctaatactt ttgcaccttt
    14521 gttcatacct tctttgatga tttcaaatgc tttttttccg ccttcttcag cgttttttac
    14581 acgaatgatt tccatagtga ttgctccttt atattttggt atagcccaat tatagcattc
    14641 tttttttctt tttacaataa ttagtataga ccaaattgca tttttttcat tttcttgaca
    14701 ataaatagga atccgtgtta ggtttcataa gatggttcaa tcaaacaatc catgttgcat
    14761 gaaaaccaag caagtgattc actgtggacg ctttatgtct gcagtttttt tatgaaaaag
    14821 gagttattat gagtatactg acattaaaca acattaccca gcaatttggc gacaaaatcc
    14881 tctatgaagg tgtcgatctg caattaaatg ctggcgaaca tcttgggctg attggacaaa
    14941 acggagccgg taaaagtaca ttgattaaaa tcatcactgg tgagatctta ccagatgacg
    15001 gacagatcca ttggcaaaaa aatattcaca aaggttacct cgatcaatat gtggaagtcg
    15061 atgaaacatt aacaatcgaa gaatttctaa aaactgcgta cgcaaaagaa tacgaaaagg
    15121 aagaaaaaat tgcttttctt taccaaacat atagcgagac cttcgatgat tccttgctgg
    15181 aaaaagctgg gaaactccaa acggaattag accaaggagt gttttatcaa atcgacacct
    15241 tagttgctga gatgagcagc gggttgggga tcgatgtctt aggtttagat tcactgttga
    15301 aaaatctaag tggtggacaa cgttcaaagg tgattttagc aaaactactt ttagaaaacc
    15361 cagatgttct gatcttagat gaaccaacca atcacttaga tgatcagcat atccaatggc
    15421 tgacgcagtt tcttcaggat tttgacggtg cgtatatcgt gatttcccat gaccaagaat
    15481 ttcttgatca gatcaccacc catatcgcag acatcgagtt tggaaagatt actaagtata
    15541 ctggccatct aaaacaagca ttgaaacaaa aggaacaaaa tcgcgaaagc tatttgcgcc
    15601 aatatcatgc tcaacaaaaa caaatcgaaa agaccgaagc ttatattcga aaatacaaag
    15661 ccggttcccg atcaacgatg gcaaaaagcc gacaaaaaca attagataaa atcgaacggc
    15721 tgacgcctcc agcttcagca agtaaagctg tctttgattt tccattctcg cctattgtca
    15781 cgacattagc agtggaaaca actgatttag tcatcggata tgatcgaccg ctacttgcac
    15841 ccatcaattt gaccatccgc tttggtgaaa atattgccat tcgaggcttc aacgggattg
    15901 gaaaatctac tttattaaaa acactgatcg gtgagattga aaaaataagt ggcgatttcc
    15961 acttcccaga taacacaaaa atcaattact tttcacaaga tctgtactgg gaaaatccat
    16021 tagaaacacc actacaatat ttaagcagtc aatttccaaa agcgactatc aaagaattac
    16081 gtcgccaact agctaaagca gggttagtta atcaattagc aagtgaacct ctcactacat
    16141 taagtggtgg ggaacaaact aaggtgaaac ttgcacagtt gacaatgaac caaggtaatt
    16201 tactgattct cgatgaaccg acgaaccata tcgatcaaga aacgaaagaa agcttacaag
    16261 acgggataca gaaatttcca ggaacagtaa tcatcgtctc tcatgaacaa gaattttatc
    16321 aagatcttgt gcaacgagtc attgagattg aaccaaaata aacgtttgat tgaatagttg
    16381 aaaaaaagga gctgtgacaa aagttttgtc acaactcctt cttctgaata aatggtggcc
    16441 aattagctga ccttcggtat tagctcaaaa aacggcaaaa tatgaggagc tatttttatt
    16501 tggctattct tattacttga tgcagaacag cttttcacaa cctcttttcg tctatttttt
    16561 attttcgaga tttctctttc ctttaaaaac aactgtttga aagaagaacg cacatccttg
    16621 aaaaacaagc ccgctaaccg ttacacctaa ccagccatac tgctgccaca ttgtcgtccc
    16681 tagcaatgaa ccagccgcac ctccaataaa gtacataaac ataaataca
//
LOCUS       DAYYCW010000065        16297 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000065 DAYYCW010000000
VERSION     DAYYCW010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.067"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(474..773)
                     /locus_tag="ACHAYA_001890"
     CDS             complement(474..773)
                     /locus_tag="ACHAYA_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8605685.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(779..1009)
                     /locus_tag="ACHAYA_001891"
     CDS             complement(779..1009)
                     /locus_tag="ACHAYA_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605686.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(1445..2011)
                     /locus_tag="ACHAYA_001892"
     CDS             complement(1445..2011)
                     /locus_tag="ACHAYA_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8605687.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(2011..2967)
                     /locus_tag="ACHAYA_001893"
     CDS             complement(2011..2967)
                     /locus_tag="ACHAYA_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM8605688.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2967..3623)
                     /locus_tag="ACHAYA_001894"
     CDS             complement(2967..3623)
                     /locus_tag="ACHAYA_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8605689.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3993..6011
                     /locus_tag="ACHAYA_001895"
     CDS             3993..6011
                     /locus_tag="ACHAYA_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8605690.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    6081..6287
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6438..8852
                     /gene="leuS"
                     /locus_tag="ACHAYA_001896"
     CDS             6438..8852
                     /gene="leuS"
                     /locus_tag="ACHAYA_001896"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM8605691.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            9200..10753
                     /locus_tag="ACHAYA_001897"
     CDS             9200..10753
                     /locus_tag="ACHAYA_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM8605692.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(11134..12918)
                     /locus_tag="ACHAYA_001898"
     CDS             complement(11134..12918)
                     /locus_tag="ACHAYA_001898"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8605693.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12933..14654)
                     /locus_tag="ACHAYA_001899"
     CDS             complement(12933..14654)
                     /locus_tag="ACHAYA_001899"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8605694.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            14754..15515
                     /locus_tag="ACHAYA_001900"
     CDS             14754..15515
                     /locus_tag="ACHAYA_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8605695.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         5325 a         2929 c         3148 g         4895 t
ORIGIN      
        1 aagagtatta tacaagctaa agcttttctt cattgccact caaatgagtg gcttttttat
       61 gtatcctttt atggattaat gaaaggatgt ttcacatagt tatatttctg tatatttgaa
      121 aagttttact ttgattttta aatagaaaga catttgggtt atattgtgag ataataataa
      181 agaagagttt aaagcgttcc ccaaaaacca ctgccccata agtgtgttac gctttaaact
      241 cttttatatt tgaagccatt aaaaagcata ccatattttt gaaaaaaagt gagaaaaaag
      301 gcttataatt ggagtggtag ttaattagtg acttattttt gattttatag cactgatact
      361 ataaaatata gatatcatca tattacacaa tcttaatact aacttaaaaa atatctcctt
      421 tcataagtat ggtgataaaa tccgttccgg gctacctttt taggtagcct actttaatct
      481 ttgtatcttt ctggatcaac gaaagtatac tttatatagt cataacgccg atgatcgctt
      541 cgagcgtcta gcacatcagt cacgatatca aacaaaaaat atacgtcttt cttcattcta
      601 gttttcgcag cagggatttt aaagtagttc ttattagaat agtagagatt aattaataag
      661 ctatcttcga ttgctaaaaa gaaaacttct gaatcccaca ccttataaaa atctttgaca
      721 aatctattcg aaggatcaaa tttaaaccat aattgcgtct cattaaaaag cataaccatt
      781 actccgatct atttttaaac ttagtctcta cctctaatat atatcgagtt tttattttgc
      841 cttcagagaa tactgtttct ttttttgcag ttacaggttg tttattttcg gaaaaagcta
      901 atatagctaa gattgaaaca tccatctgga atttatcttt tttactgctt tgatcataaa
      961 agtctgcata ttcatcactg atattttttc taataaattc ttccatcata aaaatcacct
     1021 caaaacgatt atacgaactt acgttcttgt tgtaaagcga tatttgaggg gcaaaaaagg
     1081 ggcataattt gactgatatt aagttagcta ttgatatatg aatgacatac aaaaatataa
     1141 aatcttttaa aaatgcttat atatcaatgt ttttgccatt cttttatata tctgttatat
     1201 gtcaaaaaaa tgtgttcaat ttcaatttgt atttgaataa agaggttgtg aaaaagctgt
     1261 cctgcatcaa gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgatttggtt
     1321 ggacttatta ccgcagatat aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa
     1381 cgctctaacc tgagtattag agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt
     1441 ttatttatct ttttttctca atacaatata aaataggcgg ctggttcttt tggttgataa
     1501 accgataatt cagcacatta tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca
     1561 tatccagttc tttttctcca ccttcatgac catagtagac gacaaggatc aatcgtccgc
     1621 gaggcacaag tcgtttcaat atttcttcca tagctgtgcg tgtagtctct ggcatagtga
     1681 tgattgcttt atcacttttt gggagatacc ctagattgaa aatccctgct ttgagatttt
     1741 gttgttcagc tatcacttct cctaacgttt cgtggccttg tagaaacaat gagacacgtt
     1801 cttctaactg acgctgttct agcctttcac gagtagcatc gattgcttgt tgctggatat
     1861 caaaactata gacatgacca gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc
     1921 cattgcccat cgttgcatct acgacgtgat cccctggttg caagatttct tgcagtaatg
     1981 tatggctgaa atgcaaagca gtttgcaaca tcagacgctc acctttttcc gtacatcata
     2041 ttttccttgg aagctatctc tgcgaagaag ttcttcatca attgcattca agacttccca
     2101 ttttttcaag ctccacattg gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg
     2161 atggatgatg atctcttttg gtatcatttc cagctggtcg caaatgacat tgacatagtc
     2221 aggacggctc attaattgaa ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg
     2281 catcagatgc atcaaatgga gcttgattcc ttggatatct gaatctaaaa tggtgcgtct
     2341 gacattttct ttcatcattt ctaaactttc gcctggtaat ccgttgatca aatgcgtaca
     2401 aacacggata ttatgtttgc gtaatttagc cacgccatct aaataagttt gataatcatg
     2461 tgctcggttg atcagttttg aagtttcttc aaaagttgtt tgcaatccaa gttctaccca
     2521 taaatacatc cgctcgttca gttcagcaag gtaatcaact acttcatctg gcagacaatc
     2581 cggtcgtgta ccgactgata atcctacgac acctggtaaa ttgactactt gctcaaatcg
     2641 ttctttgatt atttcaactg gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta
     2701 ttgttggaca tgaggccatt tttgatgcat catgtcgatt tctttctgga attggattgg
     2761 aagagggtct tcaggtgcaa cgatcatgtc tcccgaacca gatacactgc aaaaagtaca
     2821 accgccatgg gcaacagtcc catctcgatt cggacagtca aatcctccat cgatcggcac
     2881 cttgaaaatt ttcccgccaa actcatttcg taaagcgtaa ttccaagaat gatatctttt
     2941 atttccttct gaataaggaa aagtcattaa aagtccttct ttctgtaaga gttatctatt
     3001 tgttttcttt tttctctata cactggataa gtcaggcaca gccaagaaag actgagtaag
     3061 taaccgccga tgatatcagt aggaaaatga acgcccagat aaatgcggct tatcccgaca
     3121 cttactataa taaacatcat cagcaattga agcaatgtgc gtaaggttct tttttgaaca
     3181 aaagctggaa gcagaaatag taaagaaccg taaagaatca tactgcctgc tgcatgtcca
     3241 ctagggaaac tgtaacttgt ttcaactact aagtgcgcca atgaaggacg ctctcgcatg
     3301 aaaaatagtt tgatcaccgg gttgatgatt ccagcaatta caataaatcc agaaacgaac
     3361 caaaatgttt ctgccctttt tttcttatac cacatccata aagccgcaca agcaaataaa
     3421 atgatgacgg taagaggatt accgaattta gtcataaaga gttggtagct gttcagatca
     3481 ggataagaag ctctgacaat gcctgtaaac cactgatcga aaccagaaag ccacgacaga
     3541 tggaacttta cgatccagcc caaaatgaaa aacaggacaa gaaagaagct accagccacc
     3601 tgaaatgatg ttttatattt catacaattc ctcctttgaa gcaatgactt attatagcat
     3661 ttttggtcca gcgaaaaaaa cggtaaagag ataaaaccaa tgtatgattc ctctttgaat
     3721 aaccaatatt cttataaaaa aaataactgt aatcggaaag acattttttg gtaatgcaaa
     3781 tgaaatgtta gggaaataaa atcagtgttt ttcgaaatcc ttttaaacaa gaacgtagac
     3841 gtaggaagaa cttgaaaatg aacagaagtt gttcattacg gtaaattaca atatgattac
     3901 aaaaaacaac aaagctctta aataactttt aataaatatt aagagaagtt ataattatta
     3961 ataatagata aattgaagtt ggaggataga agatggaaaa cattgcaaga aaagaacgga
     4021 gacgtttaga acagaccaaa cgttttcgaa aagcaaaacg aggggcagca atcgtgggaa
     4081 ctgcgatggt aggctgttct gtcgctgccc cattagtaca gccggttcct gttcaagcgg
     4141 atgaaacacc tgttcagttc ggcgcaagaa tcaatacagc ggcatttatc acagaaattg
     4201 ctacttatgc tcaaccaatc gctcaagcca acgatctata tgcatcagtg atgatcgcac
     4261 aagcagttgt agaaagcggt tggggcagta gtgcgttatc tcaagcacct tattataatc
     4321 tttttgggat aaaaggcagc tatcaaggac aaaccgtcta catggatacg cttgaatttt
     4381 taaataatca atgggtaaat aaaaaggagc ctttccgaca atatccttct tttgctgaat
     4441 ctttcaatga caatgcctat gtacttagaa atacttcatt tggcaacggt tattactatg
     4501 ccggtacttg gaagagcaac acgaaatcct atacagatgc gacagcttgt ttgacagggc
     4561 gttatgcgac tgatcctggt tacgcaggca aattaaataa tatcatcgca acttacggat
     4621 tgacccaata tgatacacca gctagcggaa acgcaggcgg tggagcgact acaggtggca
     4681 ataatactgg aagtgcaggt agtactggcg gttcaagcaa ttctggtaat acaaactcag
     4741 gttcagcaac ttcgaccact tatactgtcc aatctggtga ttctgtttgg ggaatctcaa
     4801 ataaattcgg tatcacaatg gatcaattga tccaatggaa caacatccaa aacaatttca
     4861 tttatccagg acaaaaactg acaatcaaag gtagccagga aaatggctct tcgacgaata
     4921 attccggtaa caataccaat tcttcaggaa atgctggtac atcaaacgga ggacaaacaa
     4981 ctggtgcgaa atacactgtt caatctggtg attctgtttg gaagatctca aatgatcacg
     5041 gaattacgat ggctcaattg attgaatgga acaatatcaa gaacaatttc gtttatccag
     5101 gacaacagct aatcgtatca aatggcggtt cttctaatgc tggagcaaat actggctcaa
     5161 atactgggaa tacaagtggc tctagttcgt ccagctcaag ttcttctaat acagcttcag
     5221 gaacgaaata tacagttaaa gccggagact ctgtttggag cgtctcaaat aaatacggaa
     5281 ttacgatggc tcagctaatc caatggaaca atatccaaaa taactttatc tacccaggac
     5341 aacaactgac tgtttcaaac agtggatcaa caaacagcac gtcaggtaat acaggttcgt
     5401 caaccaatac aaataacagc agttcagtta atactggcag cagttcgtct caaggcactt
     5461 ataccgtgaa agcaggtgaa tctgtttgga gcgtggcaaa taaaaacggc atcacgatgg
     5521 atcagttaat cgaatggaat aatatcaaaa acaacttcat ttatccagga cagcaattga
     5581 tcgttaaagg tggaacttcg gcaaatacaa atacaggttc aactacttct gcagcgaaac
     5641 caaatacacc gaatacgtct gctacgacaa gtacatcatc cactggtaat acgatgtata
     5701 ccgtgaaagc gggcgagtca gtttggagcg tagcgaacaa acatcatatc acgatggatc
     5761 agttaatcga atggaataat atcaaaaata atttcatcta tccaggacag caagtgattg
     5821 tgaagaaagg ttcatctcaa tcaacaaacc aacaatcagt tactggttcg aaaacctata
     5881 ctgtgaaatc tggagaatct gtttggagtg ttgctgacag tcatggaatt acaatggctc
     5941 aactaatcga atggaacaat attaagaaca actttattta tccaggtcaa acgttgattg
     6001 tagctaaata aaatagctgt tcgaagaaat aacgattgaa ttccacaaaa atatttgtta
     6061 ctataaggct acttaaatat gacaacactt gaaaaggaag tagtaaaaag actgaagttt
     6121 cagagagcgc atggctggtg agaatgtgca cggatgcttt tgaactcgcc tttgagttgc
     6181 ccttgctgaa tacccgagta agcaagcggc ggtgacggcc gttacaacgt tcgaggcctt
     6241 ttcactaagg taaaattagg tggtaccgcg tagattacgt cctataaaaa gagcaatctt
     6301 tttataggat ttttttattt cttttgtgaa gtttgagaaa acaaagcaaa aggaaataaa
     6361 aataaacaga ttctgccggt tttttcgtta tatttataat agaaacaagt gttttcaata
     6421 gaacaggagg acagcaaatg agttacaatc ataaagaaat tgagaaaaaa tggcaaaaat
     6481 actgggcaaa aacgaatact ttcaacaccc atgatgatcc tgaaaagcca aaattttacg
     6541 cactggatat gtttccctat ccatccggtc aagggctaca tgtagggcat cctgaaggtt
     6601 atacggcaac ggatatcctt tcacgtgtga aaagaagtca aggatacaac gtgctgcatc
     6661 caatggggtg ggatgcattc ggtctgcctg ctgaacaata tgcgctagat acaggaaacg
     6721 atccagctga gtttacccaa aaaaatatcg aaacattccg acgccaaatc aattctttag
     6781 gatttagtta tgactggaat cgcgaagtga atacaactga tccggagtat tataaatgga
     6841 cacaatggat ctttacgaag ctttatgaaa aaggtctagc ttatgaagca gaagtagcgg
     6901 taaactgggt acctgaatta ggaacagtta tttcaaatga agaagtgatc gatgggaaaa
     6961 gtgaacgtgg tggctatgat gtagtcagaa aaccaatgcg ccaatggatg ctgaaaatca
     7021 ctgcttatgc tgatcgctta ttggatgatc tagaattagt tgattggcca gaaagcatta
     7081 aagaaatgca aagaaattgg atcggtcgtt cagtaggagc aaatattgaa tttaaagtgg
     7141 ctggtacgga taaatcctat accgtattta cgacacgacc agatactcta ttcggtgcaa
     7201 cttactcggt tcttgcacct gaactagatc ttgttagaga aattacgacc cctgaacaaa
     7261 aagctgctgt agaagcttat atcgaagaga cagctaaaaa atcagatcta aagagaacgg
     7321 atcttgcaaa agaaaaaaca ggagtattta ctggtgctta tgcaatcaat ccagttaatg
     7381 gcaaggaaat cccaatctgg atcgccgatt atgtattatc ttcatacggc acaggagcca
     7441 tcatggctgt tccagcacat gatgaacgtg actttgaatt cgctcaaact ttcggactag
     7501 aaattcttcc agttatcgaa ggtggagatg ttgaaaaagc tgcttacaca gaagatggta
     7561 cgcacatcaa ctctgaattt ctagacggca tgaacaaaca agaagcaatc gataaaatga
     7621 atgaatggct agaagaaaat ggcgtaggga aaaaagaagt cagctatcgc ttacgtgact
     7681 ggctattctc ccgtcaacgt tactggggtg aaccaatccc aatcatccat tgggaggacg
     7741 gaacagtcac agctgtccca gaagaagagc taccattacg gttgcctaag acaaaaaaca
     7801 tcaagccaag cggtactgga gaatctccat tagcaaatat cgaggagtgg gtcaatgtcg
     7861 ttgatcctgt aacaggtaaa aaaggccgtc gcgaaacgaa tacaatgcca caatgggcag
     7921 gaagctcatg gtattacctg cgttatatcg accctcataa caaaaaagaa ctagcagatt
     7981 atgaaaagtt gaagcgttgg ctaccagttg acatctacat tggtggagca gaacatgcgg
     8041 tacttcattt actatatgca cgattctggc ataaattctt atacgatatc ggtgttgttc
     8101 ctacaaaaga accattccaa aaactataca atcaaggaat gatcttaggc gaaaacaacg
     8161 aaaaaatgtc aaaatctcgt ggtaatgtag tcaatcctga tgatgtggtc gaaacttacg
     8221 gtgcagatac actgcgcatg tacgaaatgt tcatggggcc attagatgct tcgattgctt
     8281 ggagcgaaaa tggcttagaa ggaagccgta aattcttgga tcgtgtatgg cgtttgatcg
     8341 ttgatgagaa caataaaatg cgtgaccgta tcactacatt gaatgatggc aagcttgata
     8401 aagtgtatca tcaaacagtg aaaaaagtga cagaagacta tgaaaatctt catttcaata
     8461 cagcaatctc ccaattgatg gtctttatca atgaagccta taaagtggac gctctgcctt
     8521 atgaatatat cgaaggattt gttcaactac tcgcaccgat tgctcctcac atgggtgaag
     8581 aactatgggc aatccttgga aatgatggtg gtatttctta cgcaccatgg ccaacatacg
     8641 atgaagcagc acttgttgaa gacgaagtag aagttgtttt ccaagtaaac ggtaaagttc
     8701 gtgcgaaaag caatgttccc cgagacttag gaaaagatga gctagaaaaa gcagcattag
     8761 ccaatgaaat agtgcaagaa tatattgaag gcaaaacagt cagaaaagtt attgcggtac
     8821 caaacaagtt agtcaatatc gttgctaatt aattggctct ttgtcaataa ggactgatga
     8881 gttgtgcaaa atcaaatctg ggccagaatg aacttcattc tgtctcagat tttttgttat
     8941 ttcaaattga ttaattgatt gatcaaaaag gttttgattt tcatgttctc aaacacgttc
     9001 aatgtctttt tcttctgaat ggtcttgtaa gagatctctg taatcatcaa atttttgtct
     9061 attaatctta gtattttttg atgtataaac tacctgaaat tgagagtaca taccaaattc
     9121 ataaattttt tattagtggg actagtgatt gtatggattt tgacgcataa tgaatgaagt
     9181 atgagaaaag gagagacaaa tggatcaaga actatttatt atcgaagtat tatcagctaa
     9241 acaaaaaatt atcgagaaag cgaaaaaaag agcgaaaaga ataatgttca agcttttaag
     9301 cttagtattt ttctctggtt tgcttttttt cggagctgta tcgttcctat atcgccatat
     9361 gagtgcagta gtccgttcag ctgaagggat gcaaaaatta aataatttgt atcacttttc
     9421 gtggggagtt ggcggaataa caattgttgt gcttattgtt tttcttctac ttatttggct
     9481 ccgttaccat caattgtcgc gtcatttcga tcaatggctt agtcaaagag cacaacagtt
     9541 tttacataat aaaccaactt ctcaagatga tgactatctt tattttgaga agacttcgac
     9601 gagtcaagct tttcggatac aaaaaagtga ttgccagtta ttgctgagat cacttgatga
     9661 tttacctatc tacttaggcg aaagcaaatc aaaattcgga atgaacacat tccatatttt
     9721 tttactagaa aacgaaccca gatgggaaaa aagccagtct tcgcctggtg gatacctgac
     9781 aactaagatg agtacattag tcactggttt tttcctcatt cttttagctg gaacaatcta
     9841 ttatgggaga tacactcagc caaaaatgtc ttctcatata aatgctgctt ctcacatgac
     9901 aatgaactca actaacgata cgcatataca aaagacaaat gaagggttgt tagctgatcc
     9961 taagcaaaag gtaaatttga caacacaaaa gataaatgat ctcaaacttg atacagagac
    10021 gcactctctc tatatgacaa cagatagtgg tgctaattgg gtatttgtgc cgattgattc
    10081 gcaatggttg cgttttggcg aatacaccct gacaacgggg ctgattcctg aaggttattg
    10141 gatggacaac acatatgata tttccgccga tttctcatgg tttatctatt cttctgggga
    10201 tgatgtacgt ctgctgactt caagagataa tggtaaaaca tggcaaacgt ctattatcac
    10261 ccaatcagtt cagccgatac gttatcgaaa agcggtattt tatggcaacg atggtctatt
    10321 tatctgtaca tcagccagtg ggatgtctgc agaaactttg tacatttaca ctactatgga
    10381 tggcggaatc acttggataa aaagtggaag tacaatgatc aatcagccag tacaaaatgt
    10441 cagcttcgtt acaacttctt taggttttgt ttcgacacgt gaaagtttgt tttatacaaa
    10501 caacggagga cgctcattca aagaatcagt tctgtcgatt ccggaaaact atcagacagg
    10561 ggggatagat ctttttcaat caccaaatga agttactcaa ataagcgcga atacgttaga
    10621 gacaaaattt tatttgttga aaaaaggacc aatcgatgca gggaaaatgt ttgcctgcct
    10681 cttccgttca actgataacg gggaaacatg gcaattcgtt cagcaactat cgcaagtaga
    10741 acaaggagat taaagaagag agattgtgag cctgacgttc agcaccgagt cacaagaaac
    10801 aaattcaaaa aacagcttct catatttgtt gaatagtgta actcgtgatt cggagttgac
    10861 aagagcaaca agaagcgatt ttgaagtata gcttctcgta ttttacaaga tcgtgtctta
    10921 ttgccgatag gttgtctctt gaacaccgtt tattcgtaaa aagggattgt gacaagtctt
    10981 ttgtcactac ttaccgaacg aaactgaagg agctatccaa aaagtatcat tagtattatt
    11041 ctttgtgttt ctagtggcac cactagtaca tttaaaaaag ctatcgatca tggactaatc
    11101 atacatgatc gatagctttt tagataatgt ttgttacgct atctgaaatt gactattata
    11161 aagactggca taaagcgttg gtgattggag tagttcagaa tgtgttcctt tttcgacgat
    11221 atcgccattc ttcattacaa ggatcatgtc tgcattttca atcgttgaca aacggtgtgc
    11281 aatcacaaaa cttgtcctat cttttgtcac agcttccatt gcttgctgaa tcaatgcttc
    11341 tgttcgtgta tctacgctag aagttgcttc atctaaaata acgacaggag gattcgctag
    11401 aatgactcga gcaattgtca ataattgttg ctgtccttga gataacgtgc cgttttcgct
    11461 agaaatgatc gtatcatatc cgtctggcag tgttcgaata aagtgatcac attgtgcgat
    11521 ttcagccgct tcgataattt gttctcttgt ggcatccatt ttcccgtagg cgatattatc
    11581 agctaccgtt ccttcaaaca gccaggtatt ttgtaaaacc atcccaaaag aacgacgtag
    11641 ttctccacgt gaaagcttgg taatatcaag accgtcgaat tcgatctttc ctttattcag
    11701 ttcataaaaa cgcatcagca gattgactaa agtcgttttt cctgcaccag ttggaccaac
    11761 gatcgctacc gtttgttttg gctttactga aaaatcgact tgattcatca atattttatc
    11821 tgatgtgtaa ccaaactgta catttttgaa agctattttt cctttcggtt gttctaaaag
    11881 tcttgcatct ggaaggtctg gaatttcatc tgtttcatct aatatctcaa atatcctatc
    11941 gattgctgcc attgcggatt ggatcgagtt gatcacataa gaagcggtag aaattggttc
    12001 agaaatctga ttgatgtact gaagataagc ttgtaagaag ccgatcgtca caccacctga
    12061 tagaacaagc atagcaccga ataccgcact tatgatgaag gcgagttgat taatgaaacg
    12121 aatggccgga tagatagcac agttcataaa ttgagcacgt ttaaaagcgt tgtattgctt
    12181 tttatttact tcgtgtatag cagcttctgc atgagcttgc tgattgaatg ttttgaccac
    12241 tagattacca gctaagattt cttccattcg attatttaaa acacccagtt catactgatt
    12301 ctgttcagaa atctgtttgt ttttgttcgc aatcttagtt gtaacaattg aactgccaac
    12361 gatcagcaat aagacgagca aagtaagctt gctttcgata taaaacagca tcagaccagc
    12421 agcagctatt gttacgacag aagtaaaaaa ttgattgata cctgtcaata acacttgaga
    12481 aagttgattg agaccggtag ttgtccgact aatgatgtca cctacttgat gattgtcaaa
    12541 aaacgacatc ggtaatgttt tgaatttttt tgtgatttct tttcgcaccc tcaatgtgat
    12601 cttttcgctt aacgctgcca ttgtccgttc ctgaataaat gaagtgatgc tgcttaagac
    12661 tgaacagccg atcagcaata agacagggaa caacaatgct tgtttgatat cactgaaaga
    12721 aagctgtcca atacctgttt ttcttatttc tgataagaga tcatcgatcc cgattcccat
    12781 gatcaagggc atggctacga ctaataggtt cccgaataaa ctgcatagaa acaatccgta
    12841 aaaatattta tgttcaggtt tgatcatttt ccaaaaacgt atcaaagcac gcgtgttcaa
    12901 cgtttttttc ttttccatcg tatcccttct ttctatattc cttgtgattt tgcaaactcc
    12961 tgataagttt ggcaattaga cagtagttct tcatgagtac cttgtccaac gatttttcct
    13021 tcatctaaaa caatgatgtt atctgcttgt tggattgtac tgaggcgttg agcaacaatc
    13081 acgagagttt tctccgacat gtgtttatga agggctgctc ttaatgctgc atctgtttta
    13141 tagtctaaag cggagaaact atcatcgaat acatagatct ctgctggttt gatcaacgct
    13201 cgagcaatac atatccgctg tttctggccc cctgaataat tagaaccgcc ttgagcaaca
    13261 aagctgtcca atccgtcagg aagggtgtcc actagttctt ctaattgagc gatcttgatc
    13321 gctttagaca actcttctgt tgaggcatat tcatttccca tctgcaagtt gcttaaaatt
    13381 gtcccgctga ataagaaagc cttctgaggg acataactga tgtgggagcg gatctgttct
    13441 tgagtcattt gagtaagagg aataccacca agggttactt ttcctatcgt agggtcacta
    13501 agtctcaaga gaagtttcgc caaactactt tttccagatc cggtcccgcc aacgatcgct
    13561 gttgtttttc ctttaggtat tgtgaaatca atatcttcta gaactggatc agagatttca
    13621 gtatatttaa atgtgacatg ttcaaaagca gctgcatttg tctgatcaaa ggttggttca
    13681 gctactgttt tgatcgtctc ttcctcaatt tcgatctctg cttgcagtat ttcttctatc
    13741 cgtcgtaatg aggcaaacga tttagggata atgaccaata ccatagctgc gatcatcaag
    13801 taagcaagtg tcagcataga gtattcgact acagcagtca cgacaccgat ttgtaatgct
    13861 ccttctgcta caagatttcc gccaaaccac aaaatagaag cataaacgat tcccatgagc
    13921 aaaaaagcaa ttggagtgac ccaagcaaat attcggttca ctttgatcat ttgattagca
    13981 tagtcagcaa agctcttatt cgttcgcttt tcttcatatg attgattatc aaaggcacgg
    14041 atcatattaa tcccagtaaa gaattctctc aatgtgatgg tgatccgatc catttttggc
    14101 tggatactca aagataaagg tgcccctcgt ttcatcagtc gataaacaat ccatacataa
    14161 aaaacaacag agatcaatgt aacccatgtt agtaatagag aataacgaaa catcatgaga
    14221 atggtgaaaa cgcaaataac cggtgctggt aagatcattt ggcaaaataa aacaatcatt
    14281 tgctggacat tatcgacatc attcgtcatt cttgtcaaaa gagatccaga actaaaacga
    14341 ctgatatcct tcattgacag ctgttggaat ttacggaaat atttctcccg cgtttggaat
    14401 ccaaacctag cggaagccaa tgctgaaaag taactagagg caactgcagc gattgttcct
    14461 agaaacgcta caagcagcat ttcaactcca atttttttta taactgaggc ttcgccactt
    14521 gctatacctg tgtcgatcag ctttgctact aattttggta cacctaatgt accggcgatt
    14581 tctaaacaga tcatcacaaa ggtaaggaga gcgagtcctt tgtttttctg taagaatgtt
    14641 tttaagatag acatattatt cctccttttt tcctgatact tttcgattct ataccttcaa
    14701 gtaacttgaa ggtcaataga aaaaagaata aactgaaaaa gaggtgagaa aaaatgaaaa
    14761 aaatgtatac gattggccaa gtatcggaac tatttgattt gccaaaatca acgatccgct
    14821 attgggatga acaaggatta atccgttcaa gtagacaaga agagaatgat tatcgattgt
    14881 ttgatatcga tgatattttt atgctttatg atatcgtttt ttatagaaaa ttagatatac
    14941 cgatcaaaca gatgaagaat ttatatggaa aaacattgac ggagttatat gagacacttg
    15001 atgaaacaga aagaagaatc cataaagaac tggtggtgat gaaacaaaaa caaaaagaga
    15061 taagggagca aaaaaaacag ttgaaattga tgatcgatac aaacgaggaa gaatttcctg
    15121 tagaagagat cccttttgac tgtatgatct caacggagtt tgaagatatt gtggaaatca
    15181 aaaaatttct acctaactat tcaagctttg ggatgatgag tatgccagct tccagttcac
    15241 agacgatgta tggatttttt atcgacccta gtgaagtaca tttatttact caagacgtga
    15301 tttgggagaa aaaagatacg gctgtttatc gtcgattctt attgaaatcc gagatgaacc
    15361 atgcggaacg taataatatt ttagaaatca gagaaagaat gtatgaaaaa ggatggaaaa
    15421 caggagaagt gatcggccag tatctattga cgaatacgga tgaaaacaat atcagaactg
    15481 aatattatca tgcatggata gaaatgaaaa aataggaaat atgtgtttaa gataactcga
    15541 aaatgatggt tgttttagtt ataaagaaaa attttctggt tcgattacgg gtttaatgtg
    15601 ggggattatt agtcgtggat agatagaatt ttagtttttt agaggaaata tgtctttaaa
    15661 aatggaatgg ttgcaattct tagcgatata tacgatgtaa ttgtcctctt aaaaaagtat
    15721 ggaaaaataa ttgtattggg tgttatgtat aattcatggt gaactgagac taatgagaag
    15781 aaaaattcat attgttatga gtgagattgt atcagatgag aatgataaat taaataggtg
    15841 cataaaagca ttacacttaa aatacattgt tgcttacatt attacaatat gttaataagg
    15901 caaaaataaa tagatggatt gaataaacat attttttaat caaatagtcc ctactgtgac
    15961 tttcctcagc tatcggtgat actaccgata gcattttttt gcttataaaa taattataag
    16021 tatttagaga tttttcttct aacattcttc aaacaagaaa aatcacaaag gttattgtta
    16081 tttttatata ttctttttaa gaatgattgt ttttttatag aaattttaaa aaataaaaca
    16141 cgttattgat ggatagttag tttgttattt ggtaatatta tagaagttga ctggtagtga
    16201 tttatttttc gtcgttttat aaaaatgtta ttttttggaa aaattcacaa atatgtaacg
    16261 aatgtttttc tggaatttgc tgtgttaaaa agcagta
//
LOCUS       DAYYCW010000066        16146 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000066 DAYYCW010000000
VERSION     DAYYCW010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.068"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACHAYA_001901"
     CDS             226..894
                     /locus_tag="ACHAYA_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM8605696.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1414)
                     /locus_tag="ACHAYA_001902"
     CDS             complement(1094..1414)
                     /locus_tag="ACHAYA_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605697.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1451..2833)
                     /locus_tag="ACHAYA_001903"
     CDS             complement(1451..2833)
                     /locus_tag="ACHAYA_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8605698.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACHAYA_001904"
     CDS             complement(2989..4176)
                     /locus_tag="ACHAYA_001904"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM8605699.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACHAYA_001905"
     CDS             complement(4183..5214)
                     /locus_tag="ACHAYA_001905"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8605700.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5260..6684)
                     /locus_tag="ACHAYA_001906"
     CDS             complement(5260..6684)
                     /locus_tag="ACHAYA_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM8605701.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6859..7764
                     /locus_tag="ACHAYA_001907"
     CDS             6859..7764
                     /locus_tag="ACHAYA_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM8605702.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACHAYA_001908"
     CDS             7855..8532
                     /locus_tag="ACHAYA_001908"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8605703.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACHAYA_001909"
     CDS             8529..8699
                     /locus_tag="ACHAYA_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605704.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACHAYA_001910"
     CDS             8813..9709
                     /locus_tag="ACHAYA_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8605705.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACHAYA_001911"
     CDS             complement(9764..11134)
                     /locus_tag="ACHAYA_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8605706.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACHAYA_001912"
     CDS             11372..12118
                     /locus_tag="ACHAYA_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8605707.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACHAYA_001913"
     CDS             12115..12954
                     /locus_tag="ACHAYA_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8605708.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13904
                     /locus_tag="ACHAYA_001914"
                     /pseudo
     CDS             12955..13904
                     /locus_tag="ACHAYA_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            14045..14797
                     /locus_tag="ACHAYA_001915"
     CDS             14045..14797
                     /locus_tag="ACHAYA_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605709.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14816..15967
                     /gene="queG"
                     /locus_tag="ACHAYA_001916"
     CDS             14816..15967
                     /gene="queG"
                     /locus_tag="ACHAYA_001916"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM8605710.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5239 a         3009 c         3242 g         4656 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaatgc agaagcatat gtggaaaaac
    13501 taaaagaatt agatacacaa gcgaaggaaa gttttgcttc tatcgaagag aacaaaaaat
    13561 tattagtaac aagtgaaggg gcattcaagt atttttcacg agcatatgat ttgccagcag
    13621 cttatatatg ggagatcaat acagaaagtc aaggtacgcc tgatcaaatg aaagcgatca
    13681 ttgatcagat aagagcaaaa gaagtaccgg ttttattcgt ggaaaccagt gtagactcaa
    13741 gaagcatgga acgggtagca aaagaaacgg gtttgaaaat ctatgataaa ctgttcactg
    13801 attccatagc aaaagaagga gaacaaggag attcttacta tcagatgatg aaatggaata
    13861 ttgaaacgat ccatgaaggt ttgtcacaaa caaaagaaag ctgaaaagtg caaggtctag
    13921 cttgaggagc agaccttgca ttttttataa gagatatgaa aaaaaattat gctgtttttc
    13981 gtcgtacaga tacctttttt gaagaaattc cgttatagtt agagtaagga aggaaggtgt
    14041 ggctatgaat tttacacgta aagataaata tacgattaaa gaagaagtct ttcgattgat
    14101 cagccagcag gaaaataccg acaaggatca agaccttcaa tttcttgaag cagaattagc
    14161 taaattattc atagcaaatc aaaatgagac aacagaacca gatgaagagc tgtacatagg
    14221 caaatggttg tttgatgaat ttcagccttt agatgaaaat accggtatcg gttattttgt
    14281 gttcctttct ccagtcggct tcacacctgg cgtattagtt aaacaaaacg acgaaataac
    14341 cgtcaaagca tggcctgcat tattttcagt catcaaagca gaaggagtaa tcaaaaagat
    14401 ccagtcggcc tattataaaa gtcaatttat tgccaacagc aaattaatga atatgtttta
    14461 taatcgtcta gtagaaattg ctaggcctgg gaaaatcccg caaacattca cgtacacaca
    14521 gcaaaaaaat ctctatgaag aagtattgta ttcccgcgat ctgtctgagt tttcgccatg
    14581 ggaagaggta aggctccatg aactgttaag tacaaaacca gtttcattga atttttttaa
    14641 tgcattagat aacaaaagaa aattaatttc aacaaattat tgggaagtat ttgctgcaag
    14701 aaaaaaatgg atggaagaaa agaagattgc cgatcagcag atccagtttg tccagctgaa
    14761 aaaagaaaaa ctatattatg ggttatacag ccgttaacag caagcgagga agaccatgac
    14821 attaaaagaa gaaatcatac aagaaagcaa acgattagga attgacaaaa tcgggttcac
    14881 cacggcggaa ccttttgatt cattaaaaga atcgttggaa gaacaaaaag cagctggcca
    14941 tacttctggc tttgaacatc caaatatcga tgagcgtctt tatccagaaa tgacatttga
    15001 tcagccgaaa tcgatcattt ccattgcact tgcgtatcct actaggatcc atgaagaagt
    15061 gcctagggat gaaaaacgcg ggcagtttgc tcgtgcttct tgggggatcg attaccatga
    15121 tatactcaga gaccgtttga accgattgat cgaattcatc caaacaagag cagaaaaatg
    15181 gcaaaaagaa gaggaatggc gtctagctcc gcaagtagat actggagaac tgatcgatgt
    15241 cgctgtagca cagcgagctg gtttaggatt tattggacga aacggattgt tgatcactga
    15301 agagttcggt tcatttgtct atcttggtga gatcgttact aatattgtat ttgagcctga
    15361 taaaccagga gaatttggtt gcggtgactg tatgcgctgc gtgactgctt gtccgacaaa
    15421 agcattgctt ggtgacggaa gaatgaatgc ccagcgatgt ttgtcttatc aaactcagac
    15481 aaaagggatg atgccggaag aatatcgtaa aaaaatgcga aatgtcattt atggatgtga
    15541 tatctgccag ttagtctgcc cttacaatca aggaaaagat tttcattttc atgaagagat
    15601 ggaaccaaaa gtagacgaaa tatatccaaa attaaagcca atgctttcga tgtctaataa
    15661 agacttcaaa cagcaatttg ggcatttagc tggttcatgg cgtgggaaga aacctttgca
    15721 gagaaacgca ttgattgctt tagctaatct aggtgatcga acggcattgc ctgagatttc
    15781 agaatgttta acagatgtcc gccctgtcat cagaggaaca gcagcttggg ctattggacg
    15841 tctaggaaca aaagaaccgg aaaaatggct tgatgtatta gatgaaatat tagctaaaga
    15901 aacagaagaa gtcgttataa acgaattgga acatgcccag aaattattgc ggagaaagtt
    15961 aaaataaaaa aatagttaac attattcaaa gtttttgtcc agtaattgtt gtaacaatta
    16021 ctggtttttt tgtatttttt acattttttt aagagaattt gataaaaatc aaaaattttt
    16081 ttgctttttt ttatggatta ttgcagtgaa tgttacagaa tctgcgtttt tcattcaaaa
    16141 ataaaa
//
LOCUS       DAYYCW010000067        15328 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000067 DAYYCW010000000
VERSION     DAYYCW010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.069"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..1136)
                     /locus_tag="ACHAYA_001917"
     CDS             complement(93..1136)
                     /locus_tag="ACHAYA_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM8605711.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1513..2478)
                     /locus_tag="ACHAYA_001918"
     CDS             complement(1513..2478)
                     /locus_tag="ACHAYA_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM8605712.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2712..3557
                     /locus_tag="ACHAYA_001919"
     CDS             2712..3557
                     /locus_tag="ACHAYA_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8605713.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3748..4935
                     /locus_tag="ACHAYA_001920"
     CDS             3748..4935
                     /locus_tag="ACHAYA_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8605714.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4941..5297
                     /locus_tag="ACHAYA_001921"
     CDS             4941..5297
                     /locus_tag="ACHAYA_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM8605715.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5299..5637
                     /locus_tag="ACHAYA_001922"
     CDS             5299..5637
                     /locus_tag="ACHAYA_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM8605716.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    5713..5969
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6032..7066
                     /locus_tag="ACHAYA_001923"
     CDS             6032..7066
                     /locus_tag="ACHAYA_001923"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8605717.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7059..7745
                     /locus_tag="ACHAYA_001924"
     CDS             7059..7745
                     /locus_tag="ACHAYA_001924"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8605718.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7769..8617
                     /locus_tag="ACHAYA_001925"
     CDS             7769..8617
                     /locus_tag="ACHAYA_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8605719.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEEKPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            8814..9587
                     /gene="sufC"
                     /locus_tag="ACHAYA_001926"
     CDS             8814..9587
                     /gene="sufC"
                     /locus_tag="ACHAYA_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM8605720.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            9600..10886
                     /gene="sufD"
                     /locus_tag="ACHAYA_001927"
     CDS             9600..10886
                     /gene="sufD"
                     /locus_tag="ACHAYA_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM8605721.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            10883..12118
                     /locus_tag="ACHAYA_001928"
     CDS             10883..12118
                     /locus_tag="ACHAYA_001928"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM8605722.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            12105..12575
                     /gene="sufU"
                     /locus_tag="ACHAYA_001929"
     CDS             12105..12575
                     /gene="sufU"
                     /locus_tag="ACHAYA_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM8605723.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            12580..13971
                     /gene="sufB"
                     /locus_tag="ACHAYA_001930"
     CDS             12580..13971
                     /gene="sufB"
                     /locus_tag="ACHAYA_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM8605724.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14069..15211)
                     /locus_tag="ACHAYA_001931"
     CDS             complement(14069..15211)
                     /locus_tag="ACHAYA_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8605725.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         5009 a         2852 c         3265 g         4202 t
ORIGIN      
        1 gttgttgtta gaaggatatc tgcaaaatta aaaacagttg tgattcggac attgttccat
       61 gcgagaatca caactgtttt ttcatgttca ttctaaactt tgatcagcca aataaatagt
      121 aaagccatca ctgtttggag aagcccgaca gctaatccat aagataagaa gcgtgtacct
      181 tctttcatga attttgcaaa gtctaaacgc agaccaattg ccgctaaagc agtgatctca
      241 aaccagctgc tgataaaatg tgccgattgg tcaataacta caggtaaaga aaacaagctg
      301 ttgaccaaac aaaagatcac aaaccctaat acataccaag gaagcggtaa ttttcgtttt
      361 ttctgcgtat cagttggcgt ttcaaacaga gctggttgtt tcttgtgcat gaatttttca
      421 aacagataga caacaacaac gagcatcata atacgtagaa tcttaaatag catggcgaat
      481 tcgacaacag aattgtcaag taagcttgcc ccagcgacta cttgaccaac agattggagt
      541 gtaccaccca aaagtgcact tttttcaatg agatttccac caaacaactg taatccaaga
      601 aaagggagag tcaacatcat cacagtaccc agtagattga ctagtgtgat gatctgtcct
      661 ttttcttcct ctttcgcatg gatggaaggg gcgattgaag caatagcaga agaaccacaa
      721 acagcgtttc ctccagccat catcaaagaa gaagtatcgc tgaattgcag ttttttacca
      781 atcaagtaag caccgatgat agtgactgac atttgcagta acacaaaaac aattcctttt
      841 acaccaattt gagcaatcgt ttgagctgtg acagttgcac ctagtaaaaa aacagagaat
      901 tctaatagtt tgctttcagc aaattttgtg ccgattgcta gaacaggctg tttaaaaaaa
      961 gtatttccta ataaaatacc caataaaata gcaatagtcg ctccacctaa ctgtggcaga
     1021 taaatagcaa gaagcctgct gaaggcagcg atggcaaaag aaagcagtaa accgggtaag
     1081 atttcgcgga ccgctgaatc ctttacagat tttattggat gtgacaatga tttcatttga
     1141 tcggttcctt tcgtcaataa atactaaaag catactcgaa catagtagca ctaaaccagt
     1201 attttgacaa aagaacaaga ggtcgtgaca aaagtcttgt acgacctctt gtttcgaata
     1261 aacggtgttc cagaagtagc ttctacggaa ataagccgaa atttcacaaa aatttgagaa
     1321 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctttta cgacctcttg
     1381 gtaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccagtac aaaaacatga
     1441 acagctgttt ttgtttggcc gttcttatta cttgatgcag aacagctttt tcacaaccta
     1501 ttgaatcgat tattaagcga tttttttaga aacaaatttt ccatgtttgc tatgttcttt
     1561 aattaagaaa cgtacgtaat gagcaagaac gtagaagaca actacacttg caaacagaca
     1621 ttccgcaact tttaagaaat ccaatacgct actatagata cctaaactgt tgaagtaatg
     1681 aacagtagta aatatagctg ttgcagagat aacaagcggg aatgtaaagg ctgcatagct
     1741 tggataaaaa cgaagcttga acaaacgtgg aagcatagac agcacaacga aatacaatac
     1801 ttgagataaa gcgaacaatg ctacgacaag ataaaaatta ggttcagcaa agtttttaag
     1861 ataacctgtt aggcaaagag aaccaggtgc tgcaacgatc gtaatcagcg gacgtgtcgg
     1921 atgagccata tttcttacga aaaagacacg tttgatgatg atcggcaata agaacgcata
     1981 gcagatcaat gaaatccaaa agaaaatttt tccaataagt gggaagaagt tcccggcagt
     2041 tacagtgatg accccaaatc caacgtaaat gataaaccag cttggataaa ttgcttcaat
     2101 tgaaagatca gctttgataa catggtgata attgaaataa aagacaagga tcacttggaa
     2161 gataacagcg cacagccaaa tgaatttgac aattggtgca atactttcat atgaaacaaa
     2221 ataagtacaa ataaccatga gtgacatcgt aaaagttggt gaaacggagg cgatgattgg
     2281 atcatgcaac gtttgtttcg tgtgttcgaa taagataaac aatttaacaa aaatgaatat
     2341 cataagaatc atagagatac cgccagttag attgcctaaa gcaacatggt gatagtcttt
     2401 aaacagattt ccaagggaag ctaacccaag gatcaacccg cagatcggga tcggaataat
     2461 tttcaaaaag ttcttcatat ataatattct cctttttatt taatagattt tagtaccaag
     2521 atgaacgaat acattgtaaa atgaatagga atgtatgatt acgagcaagc agacagctga
     2581 ttgtcttcat ctaagttact agttggatca gcaactgttt tgttgtacat ttctattata
     2641 gatggttttt tgtatattta aatcaaatag tttttatagt aattatcata aaaagttatg
     2701 gaagtggtgc catgtttaag ttattaacga cttttcgagc agtatatgaa acaagaaatt
     2761 tttccagagc agcagaagta ctgtttttat ctcaaccagc agtctcgaat caaatcaaac
     2821 agttggagcg ggaattgaat atcgatcttt ttcaacgtaa cggaagaaaa gagatgcttc
     2881 caactaagca agccgactta ttatatgaac ggacactggt tttgttagaa gaatgggaag
     2941 aaactcagca aaaattgctc aatgaacaaa atgaagaaga agtatgtcgg atcgttgctt
     3001 cccacacctt tgctgtctat cttttgcctc agttgatgaa acatctgatc aaaaggtttc
     3061 ctaaagtgca ttttgagatc gaaatcacga attctcatga atcattagaa caagtagcga
     3121 aacatgaatc cgattttggt tttatcgaaa agccattaga gacttccggc gtacaaagat
     3181 actctttgat ggaagaccag ctagtcatcg ctggggaccc aaaaagcaaa ttgtggcttg
     3241 taagagaagc aaactctggt gtttttcatt acacgcagcg ttattttgaa gagatgaata
     3301 tccaagataa gaagatgatc gtgaaaagta acgatgtaat tgttgcgttg ctgaaggaag
     3361 gaatcggtcg ctcgattttg tctaagcgct ctgttccaga aaatgtgcca atgactccgt
     3421 tgaatttcca tcgatatttt tatttcatcc aacgaaccaa tctggtttct gatcagctgc
     3481 tagagattgc cgaagagatc cttcgataca atgaaagcac agaaacttct cacagcgagg
     3541 agaatctcag ctggtagata attgcatgta tcaaaaaaag ttctatgata cactaaagat
     3601 aagcagtagg cttttgtact gcatttgctc aagcaactgc acaaacggaa agttaatttt
     3661 tcccaaatgt ctatggaaag actttccatt atctaactgg gccgctataa taatcagcag
     3721 tatggttttt aagaaaaggg atgaaacatg gaaaaaaaat taaaagcttc gaacgataat
     3781 cgtattgatt atggcgtgat tttaccagtc tttctgttat gtttgattgg tctgctctca
     3841 ttatatgtag cacttagcca tgatccgaac aatccagcag tcttgaaagg cgtagggatg
     3901 caagttgtct ggtatttagt tggagcggca gcaatcgttg tcatcatgca tttgaattcc
     3961 aaatggatct ggaaactgac tccttacctt tatggtttgg ggttagtggt aatgggactc
     4021 ttgctcaaat tctatgatca gaatttagct gcttcaacag ggtctaaaaa ctggtttcga
     4081 ttcggtgcgt ttactttcca gccagcagag ttgatgaaga ttgcgtacat cttgatgatg
     4141 gcactgatcg taactaaaca taatacgcaa gtaaaagaac ggacgatgaa atcggacttt
     4201 tggctgattg ggaagctctt gatcgtgaca ataccggttt tagcattgat catggcccaa
     4261 gatgactttg gaacgatgct tgtctttctt gcgatctttg gcgggatttt cctgatgtct
     4321 ggaatctctt ggcgcatcat cgcacctgta gtaatcttgg cagtagtcgt tggagcagga
     4381 acgatcttct tggttacgac tgaaggcgga agagacttgc tttataaagt tggattcaaa
     4441 tcttatcagt ttgcacgtat tgattcatgg ctggaccctt tccatgatac gtcaggaatg
     4501 agttatcagc cggcccaagg acttttggca attggtaccg gtggcttgtt tggtaaagga
     4561 ttcaacgtaa gcaatatcta tgtgcctgtt cgtgagtccg atatgatttt taccgttatc
     4621 ggtgaaaact tcggctttat cggtggtgca ttcgtcattt tcttgtattt tattttaatc
     4681 tatcgtatga ttcgggtgtg tttcgatacg aataatgaat tttacgccta tatcgcaagc
     4741 ggtttgatca tgatgctgct gttccatgtc tttgagaata ttggagcaaa tatcggatta
     4801 cttcctttaa cagggatccc attgcctttc attagtcagg gaggatcctc gattttaggg
     4861 aacatgatcg gtatcgggtt gatattatcg atgagatacc aaaatgaagc acctgcaaga
     4921 tcttcaagga ggtaagaaaa ttgtacacct tttactggta cccgaaatgt tcaacatgca
     4981 aaaaagccaa agcgtggtta gaccagcatg gcgttagtta tcaaaccgtt gatatgatca
     5041 aagaagtacc gaaggcctca acgattgaga cttggctaga aacgagtcaa gtaccaatcc
     5101 gtcgcctgtt caacacaagc gggatgaaat atcgggaact tggactgaaa gatcaagtag
     5161 gcgagtttac ggcttcaaaa gcgagccaag tcttatcgac agatggtatg ctgatcaagc
     5221 gaccactgat cttacgagag gaccagcttg ttgcaattgg attcaatgaa aaaatttatg
     5281 aaggagaact gatataaaat ggcaggcaag catttgaaga aaaaggacaa cctttggatc
     5341 ttatttaatg gaaaagaata ctgtgttggt ttgacaaatg aagcgcaaga agaacttgga
     5401 gacatcacgt ttgccaatct gccaaaagca ggacaatcgc taaaaaaaga tgaacctctg
     5461 atcgaagttg aagcggaaaa agcggtcaat gaatttgcca gcccgctaac gggtacagtt
     5521 tcttctgtta atgataaaat cggtgaagat atcaatatct tgaatgattc agatgaaatg
     5581 aatgcgtgga tcttaagttt caaagatgtt gatccaaaag aatttgacga attataaaat
     5641 cagaaaatca cttgaaaaaa gttgacactg attcttccag ttgctataat cataagcaat
     5701 cctatatgaa acgagaaagc tttgaaaaga agagtacatg tcacggacgt ttgaagagag
     5761 ccctgtttgc tgaaaatggg taacgaaaat gagatggaag atggtcttag agcagagtaa
     5821 gcgagcgtaa gcaaacttac tacgggagtg cccgttacag cgcgacagta tacggaaaga
     5881 agctaagtcc aagtactgga agaggctgtt attgcgaagt aacggtaaat caaggtggta
     5941 acacggaagt tcaagctttc gtccttttgc ttaaggcaag agggcgaagg cttttttgtt
     6001 tgaaaaatta taagagaaag atgtgaatgg catgtcatta atccaactaa cgaatgtcaa
     6061 aaagacattc aatgggaaaa acggaaacat ccaagccgtc aatgacgttt cactaaacgt
     6121 tgaaaaaggg gatatttatg gaatcgtcgg ctattctggc gcaggaaaaa gtacattagt
     6181 ccgtctcctg aatggacttg agcttcccac gagcggcgaa gtgatcgtaa acgatcaaga
     6241 tattacaaaa ttaaaaaaca aagaattacg tacattccga aagaaaatcg gtatgatctt
     6301 ccagcatttc aacctgttat ggtcaagaac tgtacttgaa aatatccagc tgccgctgga
     6361 attagctggc gtaccaaaga gcaaacgtaa agaacgggca gaagaactgt tgcgtttagt
     6421 cgggttagaa ggacgcggac aagcatatcc ttcacaactt tccggcggac agaaacaacg
     6481 cgtcggtatc gcacgagcat tggcaaatga tcctgagatc ctgctttgtg atgaggctac
     6541 tagcgcattg gacccgcaaa caacagaaga agtattagac ttattgttag cgatcaataa
     6601 gaaattgaat ttgaccatcg tcttgatcac acatgaaatg aatgtgatcc gcaaaatctg
     6661 caacaaagtc gctgtgatgg agttggggaa agtcgtagaa gaaggagacg tattgactgt
     6721 cttcagacaa ccgaaaaaag aagtaaccaa acgctttgta caacaagaca tcgaaccaga
     6781 agctgattcg acagaactat tagctgaact catcaaagaa aatccaagcg gccgtttggc
     6841 aactttgacc tttagcgaaa gaaatgccaa cgagccagtc atttcccaag cgattcgacg
     6901 ctataacatc gatgtcaacg ttgtgtatgg aaagatcaaa caagtgaaag aaggttcctt
     6961 tggttcattg actgtcttga tgactggtga aaccaaagaa ttagatcaag cagaagtgtt
     7021 catcaaagaa caaggagtag gaattgaggt gatccatcgt ggataaaagc ttttcagaaa
     7081 catatctaga ttttaaacaa atcaacccgg aaacgatgaa tcaggctatc cttgatacac
     7141 tatatatgac attaatctca atggtcttag tgacgatcat cggattagtt ttgggattga
     7201 tcctttactc tattggacgc aaaaacaaac cgtatgctcg aatcatttat ggaatcgtat
     7261 cgatcatcag taatattttc cggtcaacgc catttatgat cctgatggtt ttgatcattc
     7321 catttactaa agcaatcgta ggcactatgc taggtgcgca agcagccatt ccagctcttg
     7381 ttttatccgc agcaccattt tatgcccgtc tagtagagat tgcttttcgt gaagtcagcc
     7441 ctggtgtact ggaagcagca gatgcaatgg gagcaagtta ctgggaaatc atctggaagg
     7501 tcttgatacc tgaaagtatt cccgcattga tttctggatt aacagttaca acgatctcga
     7561 tgatcggatt tactgcgatg gccggcgcga tcggcgcagg tggacttgga ggtcttgctt
     7621 ggcaggaagg ctaccagcgt ggtaatctaa cagtgacatt cgttgcgact ttaattattt
     7681 tactaatcgt atttatcgtt caaggaatcg gtgatttctt aactaagaaa acagacaaaa
     7741 gataatagga tataggggga tgtaggatat gaaaaagaaa atttttggat ttgcagcagc
     7801 gcttttgctg actgtaggat tagcagcatg cggtaacaac agcgacagca aagattcagc
     7861 aaataaagca gacgacacga cattgaaagt cggcgcttca ccgacaccgc acgcagagat
     7921 tttggaacat gtgaaaccat tattgaaaga tgaaggaatc gatcttgaaa tcgtcaaatt
     7981 cgatgactat gtattaccga accaatcttt ggaagaaggc gacattgatg ccaattactt
     8041 ccagcatatt ccttatttga acaaagaaat caaagaaaaa ggatatgact ttgtcaatgc
     8101 cggtgctgtc cacatcgaac caatgggctt gtactctaaa aaagtcaaag atgtttccga
     8161 attaaaagat ggtgcaacaa tcattacttc aaattctgaa tcagactggg gacgcatcat
     8221 cacaattcta caagatgctg gcttagtcaa agtcaaagat ggagtagatc tggaaacagc
     8281 aacattcgat gatattgaag aaaaacctaa gaacttgaag ttcaaccata cgatcgaccc
     8341 atcactttta gcttctacgt accaaaatga tgaaggtgac ctagttgcaa tcaacgcaaa
     8401 cttcgcttac ggcgcagggt tgaatccatt agaagatgcc gtattgcttg aaaaggacaa
     8461 ctctccttac gtgaatatta ttgcgactcg taaaggtgat gaagacagtg acaaaatcaa
     8521 aaaactgatt gaagtgcttc accgcaaaga agtccaagaa tggatcacag acaaatgggg
     8581 cggctctgtt aaaccagtaa aggctgatgc aaaataaggt atggaacaaa aaagatgaga
     8641 aataagtctg taatggctta tttctcatct tttttatacc tattctaatt tagaaatcaa
     8701 aattgtttaa taataaataa aagatatttg attttagtaa ggaaggtcat tgcgcttcat
     8761 tctcaattag tctaaaatga gtgagaacga aagagaattt gggggtataa tgcatgtctg
     8821 tcttagaaat aaaaaatcta catgtgtcga tcgaggaaaa agaaatcctt aaaggcgtaa
     8881 acctaacaat gaaaaccgga gaaatccatg ccatcatggg acctaacggc acaggaaaat
     8941 ctaccttatc tgccgctatc atgggcaatc caaactatga agtaacagaa ggcgaaattt
     9001 tgtttgacgg gaaaaatgtt ttagacttgg aagtagatga acgtgcgcga cttggcttat
     9061 ttttagctat gcaatatcca agtgagatac caggtatcac gaatgcggaa ttcatgcgtg
     9121 cagcgatcaa tgcgaaacgt gatgaaaacg acaaaatttc tgttatggca tttttgaaaa
     9181 agttagatga aaaaatggct ttactaaata tgccagaaga aatggctgaa cgttatctga
     9241 acgaaggttt ttctggcgga gaaaagaaac ggaatgagat cctgcaattg ttgatgttag
     9301 aacctacatt tgcaatttta gacgaaatcg attcaggtct ggatatcgat gctttgaaag
     9361 tcgttgctaa aggagtaaat gaaatgcgcg gagacaattt cggtgcattg atcatcaccc
     9421 actatcaacg attattgaac tatatcacac ctgatgtcgt tcacatcatg atggaaggcc
     9481 gagtagtcat gacagggaat gctgacctag ctaaacgtct ggaagcagaa ggctacgctg
     9541 gtatcagtaa agaattagga atcgactata aagaagaaga agcgtaaggg ggagcaagaa
     9601 tgaaagaaaa aaactggctc gattatcttg acgcggtaaa tgatttttct ttaagtaaag
     9661 gcgaacctga ttggatgaga accttccgtc aagacgcact agcgaaagca gatgaactgc
     9721 cattgccgca tatcgatcgg gtgaaattcc atcgctggtc gttatttgat gtgaaagaaa
     9781 cacaaaccat cagtgaaaca ggcacgattc cggcatttga tgcaatgaaa gacaatcctg
     9841 tgcttgtcca acaaggttca tggacgattt ttgaacaact gcctgtggaa ttagcagaaa
     9901 aaggagtcat ttttaccgac ttgttcactg caatgatcga atatccagaa ctagtccaag
     9961 aatattatat gaaaaaagca gtaaacatga acgaagacca attgactgca ctccatgtag
    10021 cttttatgaa cagcggtatt ttcctgtatg tgcctaaaaa tgtcgtgatc gatgaaccac
    10081 tcgagtcctt attcattcaa gatggcgcat ccgatgagca cttcttcaag catgtgttga
    10141 tcgtggcaga tgaacatagt gaattttctt atctggaacg tttccagaca accaaagaac
    10201 aagtagcaaa atcatcaggt aatatcatcg tggaagtcat cgctaaagct ggttcaaaaa
    10261 tcaaatattc agccgtagat caattaggag aaaatattac ttcttatatg aatcgccgtg
    10321 ggcacatttt acgagatgct tctgtcgact gggcaattgg tgtcatgaat gacggtcatg
    10381 tgatcgctga ctttgactca gatttagcag gagaaggtgc acacgcagaa gtcaaaatcg
    10441 tggcaatcag ctcgggcaga caaatccaag gaatcgatac acgagtaacc aataaagccc
    10501 ctcatacgat cgggcatatt ttgcaacacg gagtgatccg agaaaaagga acattgacct
    10561 ttaatggtat cggccacatc ttgaaagggg caaaaggtgc agatgcccaa caagaaagtc
    10621 gtgtcttgat gctttctgat aaagcacgtg gtgatgcgaa tcctattctt ttgatcgatg
    10681 aaaacgaagt aactgctgga catgctgcca gtgtaggacg agttgatcca gaagaaatgt
    10741 actatctgat gagtagagga ttgcataaag aagaagcaga gcgcttagtg attcgaggat
    10801 tcctcggttc tgtcttaaca gcaatccctg tagaacaagt tcggaaagaa ttagtcgaag
    10861 taatcgaagg gaagttgaat ggatgatcga tgctaaaaag ctacgcaatg attttcctat
    10921 cttatttcaa gacgtcaatg atgaaccttt agtttatctg gataatgcag cgacgaccca
    10981 aaagccaaaa caagtactcc aggcattagc gtactattac gaacacgata acgccaatgt
    11041 tcatcgcggt gtccatacgt tagcagaacg cgcgacccat gattatgaac aagcaagaga
    11101 aaaagtacgg gcgttcatcc atgcaaaaga aacagcagaa gtgctgttta ctcgtggtac
    11161 gacgacaagc ttgaattggg tagccagaac cttcggcgac cgctatatca aagcaggaga
    11221 cgagatagtg ctctcttaca tggaacatca ttctaatgtt atcccgtggc agcagttggc
    11281 aaaacgaaca ggtgctgtct tgaaatatat cgaactaaca gaagaaggct tcctggatat
    11341 ggaggatgca gcgaaaaaga ttacggacaa aaccaaaatc gtctccattg ctcatgtatc
    11401 caatgtctta ggtgtcttca atcctgttca agaattagct gaacttgtcc atgaaaacgg
    11461 cggggtcatc gtagtagatg gtgctcaatc tgccccgcac atggccattg atgtccaagc
    11521 actagactgt gatttctttg ctttcagcgg acacaaaatg tgcggaccaa caggtattgg
    11581 tgttttatat ggtaaacgaa aatggttaga agaaatggaa cctgttgaat ttggtggcga
    11641 aatgatcgat tttgtcgaat tatacgacag tacatggaaa gaactacctt ggaaattcga
    11701 agcagggaca ccaaatatcg ccggcgctat cgcattaggc catgcaatcg attatttgca
    11761 aaaactcggt atggacaata tccatcgata tgaagaagaa ctagccgctt atgtgttgcc
    11821 aaaattacaa gcaattgatg gattgactgt ttatggtcca caagacccga attttcggac
    11881 aggagtcgtt gcattcaatt tggatgggtt acatccccat gatgtcgcga cagctttaga
    11941 catggaaggg gtagccgtca gagcaggtca tcattgtgcg caaccgctat tgaagtacct
    12001 tgaagtatca gcgactgctc gcgcgagctt ttatttctac aacacaacgc aagacgccga
    12061 ccgtttagtt gaagcaatca tggcaacaaa ggagtttttc caacatggga ctatctaaat
    12121 tagacaattt ataccgtcaa gtgatcttgg atcattcttc ccatccgcat catcgtggaa
    12181 gcttgactga ctcgacaagc aagatcgaat tgaacaaccc cacatgtgga gatgtgatcg
    12241 aattgcaagt cgatgtaaaa gacggaatca tcaaaaatat cgcctttgac ggcagtggct
    12301 gctccatcag tacggcaagt gcctcgatga tgacagacgc cgtcatcgga aaatcaatag
    12361 aagaagcaga acatttaact gtagactttc tacagcttgt ccaaggaaaa gaagtcaaag
    12421 atcccgacca attaggcgat gctgctatgc ttggtggtgt agctaaattt cccgctcgga
    12481 tcaaatgtgc gacacttgcc tggaaagcac taggaaaagc aatggaaaac gatggtaccg
    12541 cagctttcca tcattaccac aaggaagagg agtgagaaca tgggcgtacc agaattagaa
    12601 gaatataaat tcgggtttca cgatgatgtc gaaccggttt tcagtacagg agaaggcttg
    12661 acagaagaag tgatccgtga gatgtcaaga atcaaaggcg aaccagaatg gatgcttgaa
    12721 ttccgtctga aatctctaga aacatttaac aaaatgccaa tgcaaacatg gggccccgac
    12781 ctttcagaca tcgatttcga tgccatcaaa tattaccaaa aaccaagtga caaacccgct
    12841 cgtgactggg aagacgtacc agaaaaaatc aaagatacat ttgaaaagat tggtatacca
    12901 gaagctgaac gagcttatct agccggtgct tccgcacaat atgaatcaga agtcgtgtac
    12961 cataatatga aagatgaatt cgaaaagtta ggaatcgtct ttactgacac tgactctgct
    13021 ttgaaagaat accctgaatt attcaaagag tacttttcaa aacttgtacc gccaaccgac
    13081 aacaaattag cagcgctgaa ttcagccgta tggtcaggtg ggacctttat ttacgtgcca
    13141 aaaggcgtca aagtcgatgt tccattacaa acgtacttcc ggatcaatgc ggaaaacacg
    13201 gggcaattcg aacgtacgct gatcattgtt gacgaaggag caagcatcca ctatgtcgaa
    13261 ggttgtacgg cacctactta ttcaagtaac agcctgcatg ccgccatcgt agaaatcttt
    13321 acaagaaaag atgcgtattg ccgttacaca acgatccaaa actggtcaga caatgtgtac
    13381 aacttagtaa ccaaacgagc aaaagcatac gaaggcgcaa ccgttgaatg gatcgacgga
    13441 aacctaggtg caaaaacaac gatgaaatac ccaagtgttt acttagatgg aaaaggtgca
    13501 cgaggcacca tgctctccat cgcctttgca ggagcaaacc aaatccaaga tacgggtgca
    13561 aaaatgatcc acaatgcacc aaatacatct agctctatcg tctctaaatc aatttcaaaa
    13621 gacggcggag aagtaaacta tcgaggacaa gtaacctttg ccaaaaacag tgcgggttcg
    13681 atctctcata tcgaatgtga cacgatcatc atggacgaat ggtcgaaatc cgatacgatc
    13741 ccattcaatg agatccacaa cagccaagtc tctttagaac acgaagccaa agtctcaaaa
    13801 atctcagaag aacaactcta ctatctgatg agccgaggcc tgaccgaatc tgaagcaact
    13861 gaaatgatcg tcatgggctt tgttgaacca ttcaccaaag aacttccaat ggaatatgca
    13921 gtcgaactca atcgattgat cagttatgag atggaagggt ctgtaggtta ataactattt
    13981 cattgctcga aacgttgata ttaaagcgtt tcgagcgttt ttttatttgt attttaatgg
    14041 ttggctacgt ttttggctac gtttggaatt attcgcccat aaattttgca aatcggtcac
    14101 cagttttctt aacagattgt ggtgtgacat gagcatagat attcatggta gttttaatat
    14161 ctttatgacc taatcgttcc tgtacttctt ttatcgacgc accactttca aacaataagc
    14221 tacagtgggt atgcctaaat ccatgtggag taatttgagg ctccatcggt gtctttttgt
    14281 aaatccagtc taaccaatcg tttactactt gggggtagta caacttgttt gtgtttgtag
    14341 tgaaaagaaa ctgttttggt gaattcgtgt tatagccata taagatcatt attttcattt
    14401 gttcttgttg ccaaatacgc agttgtttaa gtgtaaaatc atcaagctga atcgttcttg
    14461 atgaattttt agttttaggt acttgtagaa ctacttgatt aaactcgtct tgagcgatag
    14521 ttttaccaat tgttattgtt ttattgaaat agtctatatc ttcccattga agcgccaatg
    14581 cttcgctttt acgcattcct gtataagcta ataaacggaa aaaagtgtac agtttaactg
    14641 aattattttc tttagtattc tgtaaaaaca gttgtaattg atctttcgtg taaaacttca
    14701 agggttgttc ttcttcaatt tttcttggaa gcaatgtttt agacatagga ttatccttac
    14761 agacttccat tgctactcca aatttaaata tttgagcagt tgtttttctc aagaaaccat
    14821 attgttttaa tgttttgtgc cattcattca cctgtttttg gcaatagcta acacttattt
    14881 tatccaatcg taattcccca aaagcaggta atatatggtt tttgcaaaaa cgttgagcaa
    14941 cggcaacaga agacggtttt actcttgttc tgtagttatc aatccataat tcatacaact
    15001 cttgaaaagt tgtaacattg attttaaact cttgtttttc tttaaagtct acaagtagtc
    15061 tattaagtgc tgtttgcgcc tcagcttttg atttgaatct tctacgcttt gtacgtatat
    15121 cttttccagt cttgggatct ttccctaaat aagtttcaaa tccccaatat ttttcgccat
    15181 tttgtaattt ataactaaaa aatgttgcca tttgattctc tccttacttc attattgcag
    15241 gtagagaagt tatttgaaga atcaattatt tttaaattta tcgtgattat cagttaaaaa
    15301 ttttgcgatt atactatttt tctcatat
//
LOCUS       DAYYCW010000068        15085 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000068 DAYYCW010000000
VERSION     DAYYCW010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.070"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..716)
                     /locus_tag="ACHAYA_001932"
                     /pseudo
     CDS             complement(18..716)
                     /locus_tag="ACHAYA_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            795..971
                     /locus_tag="ACHAYA_001933"
                     /pseudo
     CDS             795..971
                     /locus_tag="ACHAYA_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1147..1716)
                     /locus_tag="ACHAYA_001934"
     CDS             complement(1147..1716)
                     /locus_tag="ACHAYA_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8605726.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1790..3079)
                     /locus_tag="ACHAYA_001935"
     CDS             complement(1790..3079)
                     /locus_tag="ACHAYA_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605727.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3406..4281
                     /locus_tag="ACHAYA_001936"
     CDS             3406..4281
                     /locus_tag="ACHAYA_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8605728.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4295..4618
                     /locus_tag="ACHAYA_001937"
     CDS             4295..4618
                     /locus_tag="ACHAYA_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605729.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4615..5367
                     /locus_tag="ACHAYA_001938"
     CDS             4615..5367
                     /locus_tag="ACHAYA_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM8605730.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5357..7528
                     /locus_tag="ACHAYA_001939"
                     /pseudo
     CDS             5357..7528
                     /locus_tag="ACHAYA_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7640..9811)
                     /gene="copB"
                     /locus_tag="ACHAYA_001940"
     CDS             complement(7640..9811)
                     /gene="copB"
                     /locus_tag="ACHAYA_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM8605731.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9831..12017)
                     /locus_tag="ACHAYA_001941"
     CDS             complement(9831..12017)
                     /locus_tag="ACHAYA_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8605732.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="ACHAYA_001942"
     CDS             complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="ACHAYA_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM8605733.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12239..12679)
                     /locus_tag="ACHAYA_001943"
     CDS             complement(12239..12679)
                     /locus_tag="ACHAYA_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM8605734.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12753..13251)
                     /locus_tag="ACHAYA_001944"
                     /pseudo
     CDS             complement(12753..13251)
                     /locus_tag="ACHAYA_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            complement(13536..15002)
                     /locus_tag="ACHAYA_001945"
     CDS             complement(13536..15002)
                     /locus_tag="ACHAYA_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8605735.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4290 a         3153 c         2678 g         4964 t
ORIGIN      
        1 aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      541 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      601 gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1021 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1081 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1141 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1201 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1261 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1321 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1381 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1441 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttgg
     1501 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttt
     1561 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1621 agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1681 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     1741 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     1801 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     1861 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat
     1921 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     1981 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2041 aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc tggagtgttt
     2101 tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc
     2161 gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc tgataataac
     2221 gaacttcttc tatcagccgg tcatggatac caattacgta acgaagccat tcttggtaaa
     2281 gtacttttat cagctttgat ttgctttcgc tctcaaattc gctattcagc caaaacagcg
     2341 aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt
     2401 tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga taattttttt
     2461 cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat tcaaagaata
     2521 gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc ttcaataatt
     2581 ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc ggaaaatctt
     2641 tcaaaaagta aaacagcaga tatcttactt ttctttctga accaaagatc tgtgcttttt
     2701 tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct tgcgtagaac
     2761 gatcgtactt ctgctcgtaa ggttcattta atagcagatt tttcagtaaa caaaacgggg
     2821 gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga tcataaacta
     2881 actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc gccaaacgtt
     2941 tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa tcagcttttc
     3001 gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca ttttttgata
     3061 atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat tgtttgatca
     3121 aaatgagttc actcctaaaa agataaaaaa ctccctttct atttatttaa atacgcaaat
     3181 cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa aacgaatgta
     3241 ctttaaaaaa acataataag atcactttgc ttgtaggatg tattcccttc cattacaatg
     3301 aaataaatag cgattttatg agaaggtgat gtgaaaatgg aagaaaaaat agaagaatcc
     3361 aaacaaaaaa tcgatcagtt gatataagaa ttagaaaaag cggtcatcgg caaacgttca
     3421 actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt cgaagatatt
     3481 cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca gggaaccttc
     3541 agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt ttccgtatac
     3601 aattcacaat caaaagagtt tgaatttcgt ccgggaccga tctttaccac gatcttgttg
     3661 gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga agcaatggcc
     3721 gagaatcatg caacgatcga caacaataca taccctctag acaatcattt ttttgtctta
     3781 gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc acagctcgat
     3841 cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact tttgctactt
     3901 ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag ttatgaaata
     3961 gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc atcttatgcc
     4021 ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat cagtccacga
     4081 ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg gagaaattac
     4141 gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg cctgattttt
     4201 tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct tgttgcacaa
     4261 attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag attatttcat
     4321 taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg tattcaacaa
     4381 ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga gcttgatttc
     4441 attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac tcgtttatcg
     4501 caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt ctttacctct
     4561 ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga gattttgatt
     4621 cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag aggatgccat
     4681 gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag aaaaagtgca
     4741 aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa
     4801 aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat
     4861 tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga ttggaaattg
     4921 tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga agaatgttct
     4981 cctctttttt gcttttgggg ttcgcccagt ccaaaatttg aacctctgct agacttgtat
     5041 ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt aggcaaccgc
     5101 gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc tggcgatgag
     5161 aaagcttttt tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct
     5221 gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg aacggcttat
     5281 cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat atgttcattg
     5341 ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg tttactcctt
     5401 tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc aaaacagcta
     5461 ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg
     5521 ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat attttccttc
     5581 gaagacattt tccatattat ggatcttaca gtttttttcg tcagttacag aggaattctt
     5641 ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt ttctcataat
     5701 gttgctgatc tatttattta ttacattgac cgtggattac gactattggt acgcaccctt
     5761 tctttctttg atggcttact tcatgatgct taccgttttt cggaaaaaag atttactgta
     5821 cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc aatttttctc
     5881 catccggttg ttgtcgggaa acactcgatt ccagtcattg actacagttc tgcttatttg
     5941 tttcttaact tttagcgcta tcttgccttt atatcttccg aaagtacacc aatctctcga
     6001 ggaaacgagt gaaccgatcc gagaatatct taatgatgaa gggctttatg acacactcca
     6061 tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg aacagcttgg
     6121 tggaccttta tatgataatg gcgaagttgt ttttacagcg aaccaaaaag gaaatggcta
     6181 ttggaagatc gaaaataaaa atcattacac cggttctgga tggatagaag atcaggacgc
     6241 acccatccaa ccaatccaag aattccctgt tgacatcacg agctaccaaa ccgttataga
     6301 agacaaagaa aacattacat tgaccctaaa aaaacaagct ttcttacctt atccttatgg
     6361 aaatactacc ttgactgatt tttcattagc tagtggacaa acgctttact atttgcctga
     6421 aagccagcga ttcattcctg tcccgaatga atcgacacgt tctgcaactt tacaaacaga
     6481 aaggttagtt tttacgagag aagaattaaa aagcagtcag ttaaaagctg atgatttaga
     6541 aaatcagcag acaaatcttc aattgccaaa aagtttgcct aaacgtattg aggacttggc
     6601 ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg tctaacagta
     6661 tttaaaaaca agcggaaatt tccgctattc caaaacggat accgggcata ctccgaaaga
     6721 acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg acaacttttc
     6781 aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga caaaaggttt
     6841 tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca gcattctcaa
     6901 tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc ttccatttga
     6961 accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa caaccgaaac
     7021 cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa ctatttccag
     7081 tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt ataaaatcgt
     7141 cttattcagc cttggagtgt gtttatccgt cttgttgttc tttctattgt tattcagaaa
     7201 atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta ctaatacgat
     7261 tgcatatcaa aaacttctta accctttgga aacattggta ccgcgcaaac ccgcagaatc
     7321 tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc gtttcattcg
     7381 attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg taaaattcga
     7441 taaaaaaacc tatttagaaa taacgcgtat aataagaaaa acggtataca aaaaaattct
     7501 gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta cttctggaat
     7561 gccgagaacc agctgattga gtatcgatta ctcgaaaaaa aggttgtgac acgttttttg
     7621 tcacaacctt ttttcattat tatttgattt tcaatgtcaa agcatttaga gctacaacta
     7681 ccgtacttaa cgacatcaag acagcaccaa ctgctggact taatatgata ccaattggcg
     7741 caagaattcc ggctgctagc ggaattgcaa gaatattgta acctgccccc caccagaggt
     7801 tctggatcat tttctttcgc gtttgcttgg ctagatcaag aaaatgcaaa atatcttttg
     7861 gatcgctgtt tgtcaatacg acatccgcag aatcgattgc gatatctgtc cctgcaccga
     7921 ttgccatccc gattgaagca cgggctaaac ttggcgcatc attgatccca tcacccacca
     7981 tgattacttt tttcccttgg tcaaggtatt tctggacaac agcttcttta tcatctggta
     8041 ataaaccgcc ataatattct tcgatcccta aataatctgc tactgcttgg gcagcgtttt
     8101 tattgtcacc agtaagcatg acaggagtga tttttctttc tttcaatgtt cggataaatt
     8161 cttttgcttc tgtttttact acgtcaccta aagcaacgat tgcagctaga tgattttcta
     8221 aaatcaggaa gctgatggta tttccttgtt cttggtaagg tttcaataat gcttgttcta
     8281 cgtttaatcc gagacgctca acttcttttt cattaactat tttcacttgt tgttttttga
     8341 ctgttgcctc tagcccgact cctgaaagat tttttagatt ttcagcttga taaggcttga
     8401 tttcatgttc tttcaaataa ttcataatgc caacagccaa tggatgattg gcattcgctt
     8461 ccaaggctcc tagatattga aggatttcat ttttattgaa cgcatcatca agtacttcga
     8521 cacccgttac agtaaatttt ccttctgtta gtgttcctgt tttgtccagc atgatcacat
     8581 ctagatcatt ggcttgctcc agtgcattgc ggtttttcaa aagtaatccg tttttagctg
     8641 ctattgatgt ggagcgagca acaactaatg ggatagctaa acctagagca tgaggacagg
     8701 cgatgataaa gactgttacc atacgttcta atgcattagg aagatctgtc aagaacaacc
     8761 aggcgatgaa tgccaggata cccacaatta atgcgacata aaacagccat tttgccactt
     8821 tgtcagataa tgactctagc ttggattttt ctccttgtgc ttttcgcacc atttccataa
     8881 ccttcgctag ataaccattt tcaccagttc cagttacttc gatttcgatc gttccatccc
     8941 cattgataga accgccaatc actttgtcat tggcttgttt tttgattcct cttgattcgc
     9001 cagtcacagc tgattcgtca acaattgttt cacctttcaa gattacacca tctgttggca
     9061 ttttatcacc agaccggacg atcaaacgat caccttcgtt tacttctttc aatgaaactg
     9121 tctcttcttt tccatctttc gtcaagcgtt tcactgattc tggcagtaat tctgctagtt
     9181 tttgtaaagc attgctggca ttcgacacag cattcatttc aatccagtga cctagtagca
     9241 taataacgat caaagtagcc aattcccaga agaaatccat gacgtgtgta tgtggattga
     9301 gcagatttgc gataaacgaa tagacactat aaatatatgc aactgtaatc cccatcgcga
     9361 tcagtgtcat catagcagga cttttttgtt tcaattccat cttagctcca cttaaaaatg
     9421 gctgaccgcc atagataaat agtatagttg ctaagatcaa tacgagccaa tcagaaccag
     9481 gaaacgttac ttgaaatgga aattccattc ccatcattgg tgaaaaaaga atgattggga
     9541 tagctaggat aagcgatagc caaaattttt gtttgaaatt ccccatgtgc atggaatgat
     9601 ccatgccgcc gtgttcgtga tccatgtgca tcgaatggtc catttgtttg ctgtttgcta
     9661 tatctccatg gttatgtccc atgtgcatgg aatgatccat ctgactatgg tcatgtttca
     9721 tctccatctg tttttcatga gattctgtac tttctttgtt tactacttct tttttatgct
     9781 catgtttttt ctccatatcg ttcccattca tttttactcc atcccttctc ttactttttc
     9841 atgtgtcgat ttaagctcag ggagttcaac aatacactga cggaactaaa cgccatcgct
     9901 cctcctgcaa tgattggatt caaaaagcct agtgcggcaa aaggaatacc aatcgtatta
     9961 tagacaaaag cccagaaaag attttgtttg atttttctca atgtcaacct agacaactcg
    10021 attgtttgtg caatggatgc caaactgcta ttcatcagcg tcacatcggc tgtttccatc
    10081 gcaatgtccg ttccacttcc cattgccata ccgacatcag ctaatgctaa ggccggtgca
    10141 tcattgattc catcgcctgc cataccgact tttttgccat ctttctgaag tttttctacg
    10201 taggctgctt tatcctctgg aagtacttca gcaaagatat gctttggatc aataccaact
    10261 tgtttaccaa tcgtttcggc tgccagtttg ttgtctcctg tcagcataaa aacatctaat
    10321 cctttattct ggagctgctt gattgcttgt ttggcttcta gtttgatttg atcagccacg
    10381 gcaatccacc cgatcacttg cttttcatca gctaaaaaca tgactgtttt tccctgctgt
    10441 tctaattcta acgcgtgttc ttggtattca tcaaatgata ggttcaattc aattaatcgt
    10501 ttacgtgtac ccgcaaaata gcgtactcca ttaatcgtac cgctgatccc agcacctggg
    10561 tgggctgtaa aatcaatcat atcgtatgtt gctgcttctt gcagttttcc gtattccacg
    10621 attgctttac ctagtggatg ttcagaagct tgttctaaag tataaaaaat agataggact
    10681 tcttttgaac cgactagatc tgtaactttg ggctttcctt ctgtaatcgt tccagtttta
    10741 tccaaaacaa tgctgtctaa gtgagcagct gcttctaatg cttctccgcc tttaattaaa
    10801 attccatttc gtgcaccaac acccgttcct accataatag ctgttggggt cgctaaacct
    10861 aatgcacatg gacacgcaat gactaataca gagacactat ggatcaatgc gagttgccaa
    10921 tcaccagtga tcaaacctgt agctatgagc gtgataaacg cgatgactaa tacgatcgga
    10981 acaaaaatcc ctgagatccg atctgcgatt ttttggattg gggctttgct cccttgagca
    11041 tcttccacca tttgaatgat ttgcgctaat actgtctgct tgccgacttg tgtgacttcc
    11101 atgtgtagca gaccattcgt attgattgtc ccgccaaaaa gcgtgtcatc cggattcttt
    11161 tctacaggaa gattttcacc agtaagcata ctttcatcta tcgcactgct tcctgaaata
    11221 attcgaccat ctgctggaat ctgctcacca ggacggatga ctagttgatc accaacgact
    11281 acttcttcaa tcgctaacgt ttcttctttc ccgtttctta tcacttgggc ggtttttgtt
    11341 tgaagagaca tcaattgttt gattgcatta cctgttttcg tttttgctgt atgttccaaa
    11401 tatttgccga gcaaaatcag cgtgatgatc atactgctgc tttcaaaata aagatcttgg
    11461 gggtgtccac ggaaaaatcc gttgtaaatg ctcaatgcaa aggcagccga tgttccaatt
    11521 gctaccaata catccatatt tggtgctttc gtcttgattg catggtacgc acctttataa
    11581 aaacgagcac cgacataaaa ttggacagga gcagacagca ctaactggac gattggaaaa
    11641 tgaaaaaagt gaacaatcgc cgcatggctt cctaacatca tagcaatcat tgacaacatc
    11701 aacggcaaag tcaaaaccgt actcaatata agatcgaata gcattttttt caaatacgct
    11761 tgcttttctt ctgcaatttt ttgtttgtgg gcttcatcgt acaaaattgc accataaccg
    11821 atgttttcta cgctttggat taatttttca gtggtcgtcc cttcatattt gacacttgct
    11881 ttttcagtgg ccaagttgac tgtggcattg accacaccag gttgtccgtt cagctctttt
    11941 tcgatcctag cagaacaatt tgcacaagtc atacctgtaa tgacgaacgt atccattttg
    12001 gtttctgctg ttgccatcaa atcacctctg ctttataacc caatccattg attgcttgac
    12061 aaatcttttc ggattgaact tttgtttcat caaacttgac cgtaccgttt gcttttttca
    12121 aatttacttt cactttttgc acgccgtcaa gtgcgcttac tgtttcttct actcttgctg
    12181 cacaatgatt gcagctcata ccttcgattg aaaattgttg tttcattttt tcttcctcct
    12241 attgatgttg tttacattca cattgacctg ggatacagtt acattcgatc gtatctactg
    12301 gttgcttttg ttccagttgt ttcaaaatct cattgatatc ttcttgagtc aaagtagctt
    12361 cttctactag atccgcaatc gtctctccaa tcctttttgc acagatatgg gaaaaaagat
    12421 tttccgtcgc actgcgaact gtttccatct ccgagacagc tggatgataa atgaattttt
    12481 ttcctttttg ttctgtccag atcacttctt tttttaccaa acgtcccagc aaagttttga
    12541 tcgttgctgg tttccaatcc atcgaatcag ctaagatctg cgtgatttct tgggctgtcg
    12601 tctgtccaag tgtccaaata acacgcatga tctcccactc tgaatcactg atctttactg
    12661 gcgttttgac tttttccatt tatttcacac ctttcgttta caaatgtaat cattagctta
    12721 tgaatacaga ttacgcttgt aaacaaaaac tgtcaattat ttttgctctc tattttcaat
    12781 ttagttaaaa attcttgttt ctctcgtctt gttttaagga tgatcagttt atttcttgca
    12841 ttagctactt ggatcagctg ctgtattttt ggttcattgt ttccttcaaa atgccagata
    12901 aaagataaaa attctaagta tttcctgttt agtttctccg aaaatgattc aggcatatct
    12961 ggccgagatt ttttgtttat ctttcttttt agagaacgcc aaactactcg gtagatggcg
    13021 agataacgcg gcactttaag ccaaatgatc ttatctgctt ctttaatcct ttcctctaac
    13081 gtagaagtat aatttccttc aacgatccat gacggattag atactataaa cgattgctgt
    13141 tcttgcaaaa accacttttt tgcctgaatg cggaaagccg gtgatttctt tgatcttcct
    13201 tgcgagggac gattttcccg acccactttg tccaatcacg atgatcttca ttcttctccc
    13261 tcacttctct ttggaatttg gttcgatcat accacttata acgccgcaaa aaaacaaggg
    13321 actgtgacaa aagtcgtgtc aaagtccctt gtttcgaata aatggtgttc aatcagctga
    13381 ccttcagcat tagctcaaaa aacggcaaaa tatgaggagc tatttttgtt tggctgttct
    13441 tattacttga tgcaggacag ctttttcaca acctctgatt cacggtgttc cagaagtagc
    13501 ttctgtcaca acctcttgta acaagtattt ctattttaag gctgaaccac tgtttctggt
    13561 tcagtaattt ctttgttttt gtctcgacga gcaattgctg gcaagatcat acctaataag
    13621 atcaagacaa ttggtgtgat gatattcgtg aataattgga accaccatgc tttaggatca
    13681 gcagtataat ctacttttgg caccattcct aggatacatg caaacgcagt gaaggcaaag
    13741 caccaagcac caaagataaa accgattttt ggatttttaa caaatttata ttcagaattg
    13801 aatttcttat aagctttatt caacatcata tatgccaaga acacccatag gtaacgcatt
    13861 ggcatgacaa ctgagttcaa gttcgtcaac cattttacta attcattcat atctttgata
    13921 ccaaagatcg gcaacaaaat aatgattcct actagtatac ctgtcagaag ataaccattc
    13981 accaatgtac ctttacgatt acgtttacgt agccagtttg gtacaaattc tggatccgca
    14041 tctgctaaca agatttttag tggtgcatca attgaaaagg ctaatgcgga gatttgtcca
    14101 atcatgtttg tcaatgcgta aatgatcatc aatgagttac cgataccata gtaattacct
    14161 aaaatttgaa aagcactgta tgccccattt gccatcaagt ctttagggat attatcgcta
    14221 gcaaacagca tacccattgc gactgagcct aatacagcac aaatccctac cattgctgct
    14281 aagaagatca tccctttagg aaattccttt gcaggatttc ttgtagagtt gacataaggg
    14341 gatatttttt ccgcccctcc aactgcaaat actagcatcg aaacggttgt gaaataactg
    14401 aaatcaaatg ttggaatata tgttttcact tgattcatat gtggtgtggc aaattccatc
    14461 gttggattga tgaacggtgc cgttacagct aaaagaataa ataagatcga catgacgaac
    14521 atcgctgttc cggccagtcc gccaatcact ttcaaagtag aaagtccttt tgttgataaa
    14581 tataaaaagg caagaaatat aataagtgaa ataactgcta ccatttgtac tgacatcgta
    14641 tttacgatat ttccatttcc ttggactgcc caaccaccag caatcagaat tgcttgaggc
    14701 ttttgtgcca aataaggaat atggacgacc cagtaagtcc aagcggcata gtatgccagt
    14761 cgtttattag atgtcgtatt ttcgacccat gaactgacgc ctcctttact atccttgaag
    14821 gctgatccta attgtccaac gatcaatgcg tatggaataa aataaatggc caaaatcaat
    14881 agccaagagg tgacaactga tactccttgc tgagcatagt tattaactac attccccatg
    14941 ccccaaacca tgttaaatgc aattaacgca acagtaaacc agcgtaattg tttgttgttc
    15001 attcgttctc tcctttatga taaaaatttg ttatccagat gtaatcatat tcaattttta
    15061 acaaacttac aagacagtgt tattt
//
LOCUS       DAYYCW010000069        15024 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000069 DAYYCW010000000
VERSION     DAYYCW010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.071"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..626)
                     /locus_tag="ACHAYA_001946"
     CDS             complement(<1..626)
                     /locus_tag="ACHAYA_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8605736.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            complement(1200..2384)
                     /locus_tag="ACHAYA_001947"
     CDS             complement(1200..2384)
                     /locus_tag="ACHAYA_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM8605737.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(2409..3416)
                     /locus_tag="ACHAYA_001948"
     CDS             complement(2409..3416)
                     /locus_tag="ACHAYA_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8605738.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(3588..3947)
                     /gene="comGG"
                     /locus_tag="ACHAYA_001949"
     CDS             complement(3588..3947)
                     /gene="comGG"
                     /locus_tag="ACHAYA_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM8605739.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(3919..4344)
                     /gene="comGF"
                     /locus_tag="ACHAYA_001950"
     CDS             complement(3919..4344)
                     /gene="comGF"
                     /locus_tag="ACHAYA_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM8605740.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(4307..4654)
                     /locus_tag="ACHAYA_001951"
     CDS             complement(4307..4654)
                     /locus_tag="ACHAYA_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605741.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(4626..5096)
                     /gene="comGD"
                     /locus_tag="ACHAYA_001952"
     CDS             complement(4626..5096)
                     /gene="comGD"
                     /locus_tag="ACHAYA_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM8605742.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(5071..5349)
                     /gene="comGC"
                     /locus_tag="ACHAYA_001953"
     CDS             complement(5071..5349)
                     /gene="comGC"
                     /locus_tag="ACHAYA_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM8605743.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(5346..6395)
                     /gene="comGB"
                     /locus_tag="ACHAYA_001954"
     CDS             complement(5346..6395)
                     /gene="comGB"
                     /locus_tag="ACHAYA_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM8605744.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(6352..7281)
                     /gene="comGA"
                     /locus_tag="ACHAYA_001955"
     CDS             complement(6352..7281)
                     /gene="comGA"
                     /locus_tag="ACHAYA_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM8605745.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            7413..9167
                     /gene="ade"
                     /locus_tag="ACHAYA_001956"
     CDS             7413..9167
                     /gene="ade"
                     /locus_tag="ACHAYA_001956"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM8605746.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(9265..9927)
                     /gene="trmB"
                     /locus_tag="ACHAYA_001957"
     CDS             complement(9265..9927)
                     /gene="trmB"
                     /locus_tag="ACHAYA_001957"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM8605747.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(9997..10779)
                     /locus_tag="ACHAYA_001958"
     CDS             complement(9997..10779)
                     /locus_tag="ACHAYA_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM8605748.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(10983..12194)
                     /locus_tag="ACHAYA_001959"
     CDS             complement(10983..12194)
                     /locus_tag="ACHAYA_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8605749.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(12187..12924)
                     /locus_tag="ACHAYA_001960"
     CDS             complement(12187..12924)
                     /locus_tag="ACHAYA_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8605750.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            13062..13496
                     /locus_tag="ACHAYA_001961"
     CDS             13062..13496
                     /locus_tag="ACHAYA_001961"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM8605751.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            13489..13833
                     /locus_tag="ACHAYA_001962"
     CDS             13489..13833
                     /locus_tag="ACHAYA_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8605752.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            14013..15023
                     /locus_tag="ACHAYA_001963"
     CDS             14013..15023
                     /locus_tag="ACHAYA_001963"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8605753.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         4759 a         3062 c         2550 g         4653 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccgtcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtagggtga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa aagtgttagt
      721 agaaaatcgc tttcgatttt ccaccaacac aaaatattat agaaccgttt tttcgcaaca
      781 aaaaacttga ttttcatacc tgtgctttca cgattcaggc aaagtacagg tggaaaatca
      841 agtttttatt attcgatcaa tgaatgtttc ggatcggttt tctagctaaa cataaagcga
      901 agaataaagt cgacagccca cgaggctgat ctaaaaatgt tttttctttt tacatatgta
      961 atgtccctta taaagatgaa tgaaaataaa atattgggat tttcctgaaa atcaataaat
     1021 aaaatgccag tcttttccct tttgaccact tttccaagca aaattcccta agaagatcct
     1081 gtaaaaaaga agggaaattg aagttaaatt accattttgc ggtcttctcc gcaaaaaaaa
     1141 cacatgcaag aattccctct tgcatgcgtt tcatctttta tcctaacgaa tgctttggct
     1201 tagccgcgca gacgctccac atcacgggca atcattagtt cctcatctgt tgggatcact
     1261 aatactctta cttttgattc ttttgtagag atatctactt cttcaccacg caattcattc
     1321 aacgctggat cgatttcaca accaaaccaa gacatacctt tgatgatttc actacgtaca
     1381 tgtccgtcat tctcgccgat accagcagta aagacaattg catcaacacc attcatcgtt
     1441 gtcacataac cgccgatgta ttttcggatg cggtccacga aaatgtcata agctaaacga
     1501 atatgttctt tttcatagtt cttttcaaga tcacgcatat cactagacaa tcctgttagg
     1561 cctagcagac cagatttctt gttcaaaaca ttgatcattt catcaatatc aatctctaat
     1621 ttttccatta aatatgggag aacagctgga tcgatatcac ctgaacgtgt acccattgtc
     1681 accccagcta atggtgtgaa tcccatagaa gtatccatgg atttacctcc atcgactgct
     1741 gtgatcgata caccatttcc taagtggcaa gtgatgattt ttagttcttc gatcggtttg
     1801 ttcaataatt ttgccgcacg ttccgacaca taacgatgac ttgtgccatg gaagccatat
     1861 tttcttactt tgaaatcttc ataataagac accggtaagc tatataaata attttgcttt
     1921 ggcattgttg agtggaaaga tgtatcgaat acagccacac tcaaaatgtc aggtaaaatc
     1981 tttttaaacg ctttgatccc cattaagttt gcaggattat gcaaaggagc aaattctgct
     2041 aattcagcaa tgcggtccat tactttctca tcgatcacta ctgaatcccc ataagtttct
     2101 cctccgtgaa caacacggtg ccctactccg gtgatttcat cataagagct taaaatttgc
     2161 agttcaatca actgatctaa cagcattttt actgctactt cgtggttttc gatatccatg
     2221 atttgttcga atttttgatt atcgccgtac ttgatggtaa aaatagaatc ttgtaaaccg
     2281 atacgttcga cgatcccttt cgcaactacg gtttcttctg gcatttgata taattgccat
     2341 ttcaaacttg aactcccagc attgattgca attgttttag acatatgact ctccttctcg
     2401 ttgaatgatt acagagcaga tgatttccag tttttaaact cgttgaaaaa ttccgtcacc
     2461 ttctctggtt cttttaatga agcaagtttc acaagcaaga cttcttttac ttgttgtgct
     2521 tgtggtcctt ttttctgtaa gatcaaaata cttttttgcg attgtttatt acggaacaat
     2581 tcatctggca attgaatcat tccttgaaga taaccttctt ctttaaacca ttttttgatt
     2641 tcttcacttt gatccgtttc taaaaatcca ctcggcatca aaaacaaacc aaaaccatcc
     2701 ggcttcacat atttcattga ttgctctagc aataaatgat gtgcataact atggccttct
     2761 tcggtacttg ttaaaaactc ttttgctttt tcgtcattcg gataataacc gattggcaaa
     2821 tcgctgattg caaaatcgac aggatctatt aataattcct gcaacccgtc ttgatgaaag
     2881 tattgaacat ttgcttgtgt caaatcactt gtagaagctg cgacagccaa caatgtatca
     2941 tcaatatcca caccaattcc ttctgtttgg taccccgcat tcgataggtt taaaagaact
     3001 gttaataaca agttgcccat tcctgccgca atatccagaa tcttaaccgg tgctttttta
     3061 tttgtgtata gttgttcaat aagaaaaaca aataaaaaac cgatactatc aggagtgagt
     3121 tgatggttcg cctgcaaatg ttctgtctgg ctgcccttca ataaaagtaa ttgagacagt
     3181 ctgcgccatt cttctggctc gaaagaaagt ttcttcaact cttcataaag agctgtgatc
     3241 cgttgcgttg tctcttttgt cggcactcca tccaccacac gtacttgata atcatctatt
     3301 aaattttctg cattttcgac ataggcttct aaaaaggaag ttcccaatgc attctgtaaa
     3361 agctgaatag cttccaggtt ttgattaaac ccttgttcca ttttttctgg taacatggtg
     3421 cacctcattt ctagatatat tataacaatt ttttcacctg tttaattcta tcgaacaatt
     3481 gacaaaattt caatcgtttt cactgttttt tatttgtgaa acggcaaaca tttttttatt
     3541 aaaaacagtg ttataacaga aaagagggcg ttttcacgaa cattccatca ttctgtacta
     3601 gtatctgtat cattttttac ttcttctgtg aaccgaaaga taaacggtga gacttccacc
     3661 tctaagaata gggactgttg tacccactga tatgttacag atccagttgt gtagtaaaaa
     3721 actccttccg aactttcggc aagcgtagac tgttccgata aagccatctg cttcattatt
     3781 tttgctatat aataattttt gattcgtata ttaagatcca ttgacaaacg gtagctttct
     3841 aatgctgcca tcaataaaaa cgtacataaa agaaataaaa gcaagacaga aaataaaata
     3901 gagcccttat aacattttct actgatcgtt ttttgtccat atggcatatc catcttctcc
     3961 attttcaaag tgtacataaa aagaaattgt gtgttctttt tccaataatt gaagctcagt
     4021 gatacttgtt aacataggtt gatgtccgcc ggaagttctt cttttacgta tcattgcctg
     4081 atatctttcg atgatatact gtttatcctc gatcgtgtag tgcagttgct gatttttcac
     4141 agagacattg tgactgctct tgagttcttc ttctaattgt gctaaaaata ttagccactc
     4201 tttttgatct ttacgctgca aaaattcact tacttggatg gtttgctgaa tcaaaagatt
     4261 gaatgtgagc aatagactgc tcaatatcat cagtccaatt agacattcaa tcagcgtaaa
     4321 tgctggaatt acttgttttt tcaatactga tcgcttcttt ctccatattg acaaacactt
     4381 tttcgattac tccattatta tcgacaattt ggatatcgta tgggatcatt tcttcccttc
     4441 gctctggtct tttgtgatac atccgctgcg tcttgatatc ttcataaatc attcgaataa
     4501 atagcagttc ctccccactt ttcttttctg cctgaagcaa aaacagctgg ccaaagctca
     4561 ctaagtagac acagacagaa gcgatcataa gcgctgtaag ggactccaac aaaacaaatc
     4621 ctgttttatt caattctttt tgtatagcga ccactcccta tctggaattg atacgtaatc
     4681 gtccgctctt tttcttccca ataaaattga taagctttta gtgaactttc attcccggtt
     4741 ctagcagcaa aagtaataga agcatggcgt ttcaaggtaa tctcatcagg aatatctaat
     4801 acttcccaat ttattttatc tggattcgga acatgaaaaa tgatttgatt ctcttcacta
     4861 ttccaataaa agcctgtttg gatctgattc acaatagcga ttttttgcgt agaatagatt
     4921 cgtttttcaa attggccgaa aaattgatag actgccaatt cattcttcca tgcagaaaat
     4981 gcgagaactg gaaaagaaag gatcgttgta agtaataaaa tcagtaagag tgattcaaac
     5041 aacgtatatc ctgatgctaa aaccttacct tcaaggccgc tgatagattt ctgcatgttc
     5101 tcgtttgata tagccttcat tcaacaattc ctccacagta ggaactcttc cattattttg
     5161 aagcgcatag agctcttttt gtgtgtctac caactggatg atcgcttcac gactttcttg
     5221 gtctacatga ttcctataac gcgacaaatt agggacgaac aacaacacta atacactgat
     5281 gatcagtaag acgattagca tttcgatcaa tgtaaaaccg ctataataag atatttttct
     5341 ctttttcatg gcaaaacctc ctctatgctt ccgtaaacag gtaataaaac agcggcataa
     5401 actaataaaa tcaaagccgc tacgcccaaa aaaatgattg gctgaataaa atgcatccag
     5461 tgatgaagac gtttgaaaag ctgttttctt gtccattcgc tgtaaaaaat cagttcttta
     5521 cctaaacttc cctttgcctc tccctgtaaa acaattttac tgaattcttc tgatagaaaa
     5581 cgataattct ggaactgctc agccaatgac atacctgcct ccaatccttg aatcaactta
     5641 gaggcaagat gctgaatcag actttcttgt ttcgtttcct ttaagcagac tatgatttgc
     5701 cttaattcga aaccttcatg aaaaagcttt ccaatctcta gtgacaaata agccgattga
     5761 tacaaggaat atatcggtcc aatcaataat tgtctgctta tccattcact ctgatcaaca
     5821 ggagacaact tcatcagacg ataccttact agtaaagcca atagagtaac cgctaggatg
     5881 aatagtagaa cataccaatg aaagacttgt atcagctgaa taccccaatg ctcttgcatg
     5941 accattcctg aaccaataag ctgaggcaaa acgaacaacc gcatactaaa caagatacct
     6001 agcaaaaagg tgagcaatag catcggatat gcaagcagct ttttcaaatc attgcgaaat
     6061 ccttcaacaa gccgaaactg tttggcaatt tccaacaaag ttgtcggcag attcccatga
     6121 cgttcagcta actcgatctg taccagttgc tctgctccat atcctatttg cgaaagtacc
     6181 tcactaaacg cttgcccttc ctttagctga tgttggatat ttgccaaaat aacaggtgga
     6241 aacgaacgga tcgtgagtaa taaataaagg gattcttgca gactaaaacc agataaaagc
     6301 agttctccca tggtttgtgc aaactggttt tgctggcttc ttgttaatcc tttagaatag
     6361 ttctgactgc caaatatttt cctgagcttc ttcataactt tcctcccatg atttttttac
     6421 tgattcttcc tccataaatt gataactcca taaaacagaa ggatggtgat tggcatcttc
     6481 caaaagttct tgataaatga caccagctaa acattcagat agctcttctt ttttctttgt
     6541 cagttcatgg attctgacca atgtaccttc aagattgcgt gcatgtacgg ttgcaaaaac
     6601 ccggtgccct gttagtgctg ctcttaccgc agcatgtgca gttgattcat ctcttatctc
     6661 gccaatgatt agcaagtctg gacgatgccg taatgcgagt ctcaaaagtg tatcataagt
     6721 ctgtgctatt ttcggattga tctgtagctg cagaaaaaac ggttcttcta tctctacagg
     6781 atcttcgata gtaatcactt gcaatttttc ttccaaagcc aaccgataca ttagagaagt
     6841 cttgcctgat ccgactggtc cgctgaaaag gtataatcca cgtctgacgg ttttctgtaa
     6901 aatagttgca aaatctcgag aactaaagca acgatggggt gtttcaaaag taccgtataa
     6961 tagccgaatc actaaactct ctcgttgaag gtaatctcct acagaagaca accgcagacg
     7021 aacttcctgt ttttctaaag aataagtaaa tgcacctaac tgagcttttc ttttttctcc
     7081 gatatccatc tgtcccaaat acttaaatct tgagatcagt tgctgtccat ctgccgaaga
     7141 gactttcttc cagaatgttc gtttttttcc atagcgaaaa tagatgtaaa atacttctga
     7201 agctggcaat atatatacgt cttgggcatg tttggcaaaa ctttcactta tcatttgatc
     7261 cgataatcgt tttatttcca ttattctccc ctccacaaaa aacatacgtc tttttgatct
     7321 ttttgtttca aataaaatga atttacaaaa aaatattggc aaaaaccgaa tttatgttat
     7381 acttataaaa atttatcttg ggaggcactc cattggaaac aacattaagg aaatatatcg
     7441 aagtagcaag taaaaaaaag cctgctgacc tagtgatcaa gcatgctaag atcgtcaacg
     7501 tctttacgaa agaaatcatg gaaaaagatg tagcaatctg cgaaggaatg atcgtaggaa
     7561 tcggtgaata cgaaggaaaa agcgtacata atgctaatgg gcaatatcta gtgccaggat
     7621 tcatcgatgg ccatgtgcat atcgaaagct cacttttatc accaagcgaa ttttcaaaag
     7681 tatccttgct tcatggagtt actactgtag tgacagatcc ccatgaaata ggaaacattg
     7741 ctgggagtac aggtcttgag tttatgatag aagatgcaag acaaacactc atgaatatct
     7801 tttttatgct accttcttgt gtacctgtaa ccccttttga gaccaatggt gcaaatttaa
     7861 atgcaaaaaa attagcccct tttttacaaa aacccgaagt tttaggctta gctgaagtca
     7921 tgaactatca agcagtagca aataatgaaa cagacatcct agaaaagatc caattgatgc
     7981 accagcataa gaaaaaaatt gacgggcatg cagctggtat cggcatggaa gagttaaatg
     8041 tttatcctgc agcaggtatt cggacggatc atgaagctac aactgcaaaa gaagctaaag
     8101 aacgtttgga tcttggtatg tatttaatgg ttcgagaagg gaccgttgca aaagatctgg
     8161 catcgctact accggcgatc acacctgaaa attcaagaag atgcttcttt gtcaccgatg
     8221 ataaactgat caatgattta gtaaaagaag gatcaatcga tcatatcgtc cgtcaagcta
     8281 ttgcacttgg aatcgatcca ttacaagcca ttcagatggc aacattgaat gcagcagaat
     8341 gttttgattt gaaaacacta ggtgcgattg cccctgggta tcaagctgat ttcttcttaa
     8401 cagacgatct taccaagctg ccaattcatg aagttttcta taagggaaca ttagttgttc
     8461 aatctgggca actccaagac catttatttg ctgatcattc aaattcatat acgtgttgtc
     8521 tgccaaaact aaacgctcaa ccattaaatc cacattcgtt agagctgctt ctttcttctt
     8581 cacaagccca tgtgatcgaa attatcccga atagtctagt gactaaagat ttggtagaaa
     8641 aagtggaccg acaaggcgaa cgattcattc cttcaatcga aaaagaccaa ttaaagattg
     8701 cggtgatcga gcgtcaccat caaacgggaa acattggatt aggcatagtg aaaggatttc
     8761 agctgaaaca aggagcgata gcgacaacga ttgcccatga ctcgcataat ctagttgtcg
     8821 ttgggacaaa cgacgaggat atgctgtatg ctgctaatct tttgattcaa aaaggtggcg
     8881 ggatgaccgt tgtcaacaat cagcaggaaa tagcttgtgt ttctcttcct ataggcggaa
     8941 tcatgacaca ggaacctttt tcgatcgtct atgaacagtt gaatgaatta ataactaaag
     9001 cacatcaact aggagcagct aaaacctttg atcctttttt gacactttct ttcttaacat
     9061 tatctgtcat acccgaattg aagatcaccg acaaaggatt attttcattt tctcgcttcc
     9121 aattgattcc accttgtgga aaaaccgatg aaataaatac attttaaatc aaataaatcc
     9181 cagaaaatac attacatctg taatgttcat tttctgggat ttatttatgg atattaatgt
     9241 ttttatgaaa gcagaaagtt agtttcacag acttttcttt ttggtcttat cttgaaattg
     9301 agcttctact cgataaatac gttgaccttt tgaagaaaat ttttcttcat actccgtcat
     9361 aatattgcct tcaaattgac tttgatgtaa atctaaccat acctgtttga ggatcatgcc
     9421 atattgagaa aaacttgcca gagaatactc aaacaatcct tgattatctg ttttgaaatg
     9481 gatttctcca tttggacgaa gaatctgttc atctacagca agaaaagtct tatatgtcaa
     9541 tcgccgtttt tcatgacgtt tttttggcca tggatcggaa aaattcagat agatttgatc
     9601 tacttcgctg tcagcgaaat actctgtcaa cgctgatcca tccacatgta gaagtttcag
     9661 gttaggaagc ggttgttcga tcaacttgtc tagtgcaatc gaaacaacac tgacctgcat
     9721 ttctactcca atataattga tttcaggatg ggcttttgcc attccagtga tgaactggcc
     9781 tttccccata ccaatctcaa tgtggattgg atgatcgttt tcaaacaatt cattccattt
     9841 tcccttccac aatgttggat cactaattac aaaattcggg tgagccgcta acatttccgc
     9901 tgctcccgga cgatttcgga cacgcattat ttatctccat tcctttcctc taattaaaaa
     9961 ggtctggcca aatctccgca gagtttgggc caaacctcag ccactgaaaa ttcgttttaa
    10021 ttgtaaaatt tcttcgttca tccgtcgatg atcttcacgt ttgcaatatc tggtgatttc
    10081 ttggagcaaa ttcatggccg cataccaatg aaatttttca agcatttcgt cagtcgggcg
    10141 tatgccataa ctgatcagcc actgattcca gctagacagt ggcacataat ttcctaaaat
    10201 cgttgcaata tccaccgcag ggtctgcaaa catgattgaa tcccaatcga ctagatataa
    10261 ataattttta catactaacc aattacgatg gtttacatct ccgtgtacta ctgataagtt
    10321 ttccttatct attgacggga tctgctcacg aagaaaacga tggacaattt gcagaaattg
    10381 atttttctgg agttcttccg gtaattcttt ttcatatttt tgcagcatct tctccggcgt
    10441 catgacacga ccaccgatac gggtcagcat ggttttaaga gaatttgaat gatgtaaatg
    10501 gtacaaaaca tcaatcacgt cgtttctttg accaatctca ttaggagtca gcaagcgacc
    10561 ttccagccac tcttgtgctg tcaaagtatc gccattcccc gttcgttttg tccaaacaag
    10621 ttttggtgta attccttccc ttgaaagagc agccaacatc ggagtcgtgt ttcgtttaat
    10681 aaagactttg tcctcatcac gcatgcctat gtatgttttt cctgtgtcgc ctttgatggg
    10741 acgtaaccgc cactcttggt ctaactgaaa ctccatcatg cattctcccg tcctcttact
    10801 ctttgttttg cagtacttat tattctagtc gcctgaacac tacacgtcaa gcacatatac
    10861 gttctttttc atttaattgc cattcaaaaa accagctttt ctcaaaaaaa atagaacgaa
    10921 cagcattctt actgtcaacg ctctattttt cattctcatt tttaccctac acagctttgt
    10981 tttcagtctg acatcttctt caaacgagtt ggtacatata gtttaacaaa gagcatagta
    11041 acaatcaaat aagccgctag cggaacccat cgctgctgcc catctaatac gactgcagca
    11101 atcaaaccaa aaataaccga cgcgataatt aatacccatg tgagtagata ctgaatcgcc
    11161 tgcttcttct gattcttatg gattggataa agctgaacga gaaccatata gcggaactgg
    11221 ttcgctaaag gtaacagttg gaaaccgatc aaatataaaa ataagaaacc gataatcaga
    11281 ctaaaataaa agtcgactac tccggcaatc aataacccac ctataattac taatcggaaa
    11341 taaagaccac taaaatcaga acctcttgcc aatcttcttg caaacaagta aagataagtg
    11401 ttttttgatt ctcttttaat atgagtcaat ataggatcca aatagcgtct tcttttgatc
    11461 tttgccgtaa tttcaggaac atctgtgaat aaattgataa attgatagat ctgatgcaac
    11521 ctatgttctt ctacatgcac cattttctcc caatccaatc ctttttcttg ttgcttccac
    11581 aaaaaccagt aatatagact tgcctgtact aatgcacctg ctattcctgc ccatattagc
    11641 caataaaatg atagaaacaa aaagatcaga gaagaaaaaa accagcctaa tttccaataa
    11701 cgaataactt gttgatctag tcgaaacaat tctgctctct tgatttgcag atgactgaat
    11761 ttcaaactcc ataatatgat cagatagacg aaaaacatcg aaaaggattg ctccgtacta
    11821 accaccacta atggcatggc caatccgcat gccaatatca aaaagatcaa cggaaaaatg
    11881 caagaatagc gaaaagctcg ttccaaatag gtttgcattt ctttttcttt aggaagtaaa
    11941 aaaacaatat ctgctgcctc tgcaaaagta gccatcttac ccacgaataa acagagcatc
    12001 caaaatacag agattatgag ccctccaatt gcaaacggtg gctgcagggt tttcaaccaa
    12061 ttagaataat ataatcccac accgccaatc aagaaaagac aaactaaaac aaaatgatcg
    12121 ttcaatacat aacgcatata tttcatcatt ttcttttgat gtcttgctaa acgtttttga
    12181 aaaaaactac tcatgtgcct gctcctttgt taaagcaata tagatttcgt ccaaagaaga
    12241 atccggcagc tgaaattctt ttctcaacat ctcaagagaa ccgtctgctc gtatctctcc
    12301 gttatgtaag accacaaaac gatcacaata tttttctgca gtagctaaaa tatgcgtaga
    12361 catcaaaata ccggctcctt cttttttcat ttctaccatc aattcaagca atgcattgat
    12421 agctagtgga tccaagccta aaaatggttc gtcgatgata tataaagaag gctcgatcaa
    12481 aaaggcgcat aagatcatca ctttctgttt cattcccttt gaaaaattag ctgggaacca
    12541 gtcaagacga ttagataacc gaaaagtctc caacaacttt tctgctcttt tcattgctat
    12601 atccataggt atatcgtagg ccatcgcagt gacttcgata tgttctctca atgtcaattc
    12661 ttcatataaa gaaggagtct ctggaatata cccaatttgt ttgcgatatt cttccgtatt
    12721 tttctttaag gatagatgat tgatttctat cgttcctttt gaagcttcca gcagaccgat
    12781 aatgtgcttg atggtcgtac ttttccctgc tccgttcagt ccaatcaacc ctactatctc
    12841 tccaggctgt acatcgaaac tgatatcttt taacacaggg agatgtccgt acccccctgt
    12901 gacatgatcg ataattaaac tcattctttt tcctccgcca tttattttta tttattatac
    12961 cacgaagaaa atgacggtat tttgtttttt tccttacaat agctttaact tatggtaaag
    13021 ttaagctgaa gtatttattc tttatagaaa ggatgtttta tatggaaaac tgtatttttt
    13081 gcaaaatcat caatcaagaa atcccaagct ataaaatata tgaagacgac aaggtctatg
    13141 cttttttaga catctctcaa gcaacgaaag gacacacatt agttgtgcct aaacgtcatg
    13201 tagcagatat ttttgaatat gatcccgaac ttgcaggaga ggttttttcg cgtgtgccta
    13261 agattgctca agcattggaa aaagcttttc cagaaatgaa aggtcttaat atcttgaata
    13321 acaaccgtga actagcctat caatctgttt ttcattcaca tatccattta gtccctcgct
    13381 acagcaaaga ggatgatttt tctattcact ttgtcaatca tcaagatagt tatggatcgg
    13441 aagaactaaa agctattcaa gaaacaatcg taaagcaggt gtcctgcgat gattaaaaac
    13501 tttttcaaag gactgttatt tggagctgct gctggcactg tcggaggtct gttattggcg
    13561 cccaggagcg ggaatgaaac aagaaagaaa ctgatcgaag aattggaaga agcaacaact
    13621 ctgacaaatg atttaaataa cagcctgaat cattttaaaa gcgcattgat cgaaacaaaa
    13681 gaaacagctg aatctgtgat tcctgctttc caagaagcaa tcaaaaaaga tatcgaagat
    13741 tttaaattcc aagccgaacc acgtatcaca caaatcaatg aacaagtaga aaaactaaaa
    13801 gctgatctgc cagagtatcc tgaacaaata taataatgat tattttctca acagatttaa
    13861 ctatataagt gagaaaagtt tgcgtatttc ctaggaatgt gatgaaattt tgaattttgt
    13921 ttgaaactac cacgacaacg tttttaaagg aaatgggata tggtatagtt aatatataaa
    13981 aataataact aattacagga gtgcaaattt taatgaaaaa aaaatcaatt attttggcag
    14041 ctactagtgc gttggctgtt ttaactctag cagcttgttc aggcgatact aataaagata
    14101 tcgcaacgat gaaaggcgga acgatcactg tatctgattt ctatgatgaa gcaaaacttg
    14161 aatcatcgaa ccaatcctta gtacaacgga tgatcattta taaagtattc aataacaaat
    14221 atggcgataa agtaaccgat aagcaagtgg atgcagaata cgacaaacaa gcgaaatcat
    14281 taggtgatac ttttgaatca caattagaag ctgctggata tacgaaagat acttataaag
    14341 aatatattcg taacaattta gcttttgaag ctggcttgaa agctcacgta gatattacag
    14401 atgacgattt gaaaactgct tggaaatctt tccatccaga agtagaagca caaatcatca
    14461 aattatcaag tgaagatgaa gcaaaagatg tgaaaaaatc agcggatgat ggagatgatt
    14521 tctctaaatt agccaaagac aaatcaactg atacagagac aaaagaagat ggcggtaaag
    14581 ttaaattcga ctcaacgaca acaactattc ctgcagaagt aaaagaagca gctttcaaat
    14641 tgaaagacgg tgagatctcc gatgttatta ctacaacaaa tcctacttct tatgcgacag
    14701 aatactacgt tgtcaaaatg gtgaaaaacc aaaataaagg caatgacatg gataaataca
    14761 aagatcaact aaaagatatc gctactgaaa ctaaactaag tgacaatgca ttcacaacta
    14821 aagtaatcgg tgaagaatta aaagatgcaa atgtaaaaat caaagatgat gcctttgaaa
    14881 atgtgttgag tgcattcaca acaacttcat caagtacgaa agactcaagt gaaactacag
    14941 cttcaacaaa gagttctgac acaaaatcaa ctgattcaac aaaagaatct tcaactgaag
    15001 aaacaactga ttcatcaaaa taaa
//
LOCUS       DAYYCW010000070        14747 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000070 DAYYCW010000000
VERSION     DAYYCW010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.072"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            443..1135
                     /locus_tag="ACHAYA_001964"
     CDS             443..1135
                     /locus_tag="ACHAYA_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8605754.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            1132..1899
                     /locus_tag="ACHAYA_001965"
     CDS             1132..1899
                     /locus_tag="ACHAYA_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8605755.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            1999..3567
                     /gene="liaX"
                     /locus_tag="ACHAYA_001966"
     CDS             1999..3567
                     /gene="liaX"
                     /locus_tag="ACHAYA_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM8605756.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3864..4184
                     /locus_tag="ACHAYA_001967"
     CDS             3864..4184
                     /locus_tag="ACHAYA_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM8605757.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPFARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4184..4534
                     /locus_tag="ACHAYA_001968"
     CDS             4184..4534
                     /locus_tag="ACHAYA_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM8605758.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4701..5639
                     /gene="hprK"
                     /locus_tag="ACHAYA_001969"
     CDS             4701..5639
                     /gene="hprK"
                     /locus_tag="ACHAYA_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM8605759.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5654..6484
                     /gene="lgt"
                     /locus_tag="ACHAYA_001970"
     CDS             5654..6484
                     /gene="lgt"
                     /locus_tag="ACHAYA_001970"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM8605760.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6528..7553
                     /locus_tag="ACHAYA_001971"
     CDS             6528..7553
                     /locus_tag="ACHAYA_001971"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8605761.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7568..8506
                     /gene="galU"
                     /locus_tag="ACHAYA_001972"
     CDS             7568..8506
                     /gene="galU"
                     /locus_tag="ACHAYA_001972"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8605762.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            8631..9071
                     /locus_tag="ACHAYA_001973"
     CDS             8631..9071
                     /locus_tag="ACHAYA_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM8605763.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            9074..9676
                     /locus_tag="ACHAYA_001974"
     CDS             9074..9676
                     /locus_tag="ACHAYA_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8605764.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(9735..10841)
                     /locus_tag="ACHAYA_001975"
     CDS             complement(9735..10841)
                     /locus_tag="ACHAYA_001975"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM8605765.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            11051..12052
                     /gene="ccpA"
                     /locus_tag="ACHAYA_001976"
     CDS             11051..12052
                     /gene="ccpA"
                     /locus_tag="ACHAYA_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM8605766.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(12389..14734)
                     /locus_tag="ACHAYA_001977"
     CDS             complement(12389..14734)
                     /locus_tag="ACHAYA_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM8605767.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
BASE COUNT         4704 a         2618 c         3079 g         4346 t
ORIGIN      
        1 ttattaccgc agatgcaagc ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa
       61 catgtcacaa acccttttca tcatttcata gaaaggataa agaatgcaaa aaaaaattag
      121 catcatcact atatttttta tttgtgtgct tacttcatgg gttattcagc atcaagccgc
      181 tttatatgaa gtacctgtca tcgagataga aaaagtacga ataaaagagg atcatcaatt
      241 gattacagga aaactgatga acacggatca aaaaggagaa tcgatttatt tatcggttcc
      301 ttatcaaaaa aatcaaatag aaacagcaaa tataaaagcg agacaaatgt atctagtcca
      361 aatgcatgga gatgaatggc agctagtagc aaaaaaaaat gatggctggc ttttcttctt
      421 cacaagtatc ttcattggcg ccatgttgct tattggtgga aaacagggtc tatttgcttt
      481 aggaagtctc atcatcaata tcgttttact tttacttttg ttgctcatct ttactaaaac
      541 agaagggatt tctctattaa atatatctat cctgttcgtc gttataggga tgattattag
      601 catattggct ttagatggat ggaatagaag cagtataatc aaaatttcag ctgcagttac
      661 gagtgttttt ttagcatttt ttatttgtct tttgacaatg aatcattgga aagaccaagg
      721 actgcgctat gaggaactga attatctgac acgaccgtat cgttctgttt ttctttctag
      781 cgtactgatt ggaacagctg gaggaacact cgataccgtt atcacagtca ttgcgacatt
      841 agaagaattg actaagcgac aaccagaaat aacagcgaaa cagttatggc tttcaggaag
      901 gcaagtgggt cgagatgtga ttggctcgat gaccaatatc cttttgttcg tctatatcag
      961 tgggtcagtt ccatcgcttc ttttatattt aggaaatcaa tggtcgttca aagaaacgat
     1021 agaacagcat ttatcattgg aatttttacg cgtcattgct ggcagcttag gaatagtatt
     1081 atcgatccct atctcggtcc tcttttttct gactgtaagg aggctgaaac gatgaatgta
     1141 cttattatac ttgttgggat tttagcaatc agcgtgctac ttattggcgg tacgcaaggt
     1201 atgtatattc ttttaggctt attcatcaat ttagggatct ttttcctgtt gttattcggt
     1261 tatcatcaga agtggccgat actggcattg tcggtcatag gatttttatt gattgctgtg
     1321 gttatcttat tttttatcaa cggttataat ctaaaaatgc gtgcagcttt tgtgagtata
     1381 ctgatctttt tattttgttt cctactattg cttcctatca ctgatttttt agcaatccaa
     1441 ggttttacat cggtagaact agaagaatta tctggcttag ataagacttt agcgattgat
     1501 tttagattac ttacgagatc gttgcttctg attagtctta gtggtgctgt tttagatgcg
     1561 agtgtagcaa tttctagtgg gacattcgaa gtatatcagg caaatccgca attatctttt
     1621 taccaattgc gccattctag tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg
     1681 acacttcttt ttgcattcat gggaagttct ttagcgttga tcctctggtt tattgattta
     1741 gacattcctt ttcagcaagt gatcaatgaa aaatcatttg ttctagaata tacgatggct
     1801 attcttacca cattgtccgc actacttgtt cttcctttaa cctgcgtgac tacagcatat
     1861 ctctttacaa aaaagaaaga acaactcaag atggagtaaa agttacaact taagtcctat
     1921 gacattttta ctgaatcagt gcataatatg gatgtaagga aaagcttgca aacaattttg
     1981 cttaaggagg caatttccat gaaagaaaga gaacgtattt tagatttagt gaaaaaagga
     2041 atcctttcga ctgaagaagg cttagattta ttagaaagca tggcaactga aaaagacgaa
     2101 aagcaaatca aaaaagaagc agacaaggta actgctagtc ataaagaaaa agatcaggca
     2161 agtcaattga tcgataaact tgaaaacggc gaagaagaaa tttctgagcc agttgatcca
     2221 aaggaaagag aaagacaaga tcaagaaaac ttagaaaaga tcttagatga actagcaact
     2281 gaagcgaata aaacttcggc gcgtctagat gaagtaaatg ctcaattcgc tgacaataag
     2341 gcagcacgca atgagaaaca ggaagcattg atgcagctga atacaaaaga agaattaggc
     2401 gagttgacag aagaagaatt agcacagcgt cagacgttag aagcagaaat caaagaatta
     2461 gaagctagtg gcgatacact gttagaagaa caattgaaat tagaagcaga attaaaagat
     2521 atccgtaaga atcaatggtc tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca
     2581 gatgattgga aagatcaagc aacagataca ctgaatcaag taggcgaaaa aatgtcagaa
     2641 gctggatcac agttaggcaa attcttgaag aaaacattcc aaactgtttc agaaacagtg
     2701 aatgacaaca tggaatggaa agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa
     2761 tttgagcatg aattttacta tgaagctcct gcagcaagta tccttgatat caaggcagca
     2821 aacggaaacg tgacgttaaa aacgtgggac agcaacgatg tgaaagttga agctaagatc
     2881 aaactttacg gaaaaatggg tgctgaacca tttgaagcat tctctgaacg gagccagatt
     2941 gaggttaatg aagatcatat ctccttccaa attccaaata aacgtgtgcg tgcagacctt
     3001 gttttctact tacctaaacg agtatacgac cacgcagcta ttaaacttct aaatggcaac
     3061 atcatgattg aaacattgga agctaaagat atttatacaa aatctacgaa tggtaacatc
     3121 atcgtggacc aattaactgc aacgatgctg gaagtagaag gtgtaaacgg caatatcgat
     3181 attcgaaacg ggaacatctt agattctatt atcgaaacag taaatggtac agtgacattt
     3241 ggtgcgacag cagaaaacct ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt
     3301 aaagaagata atttgaaaaa agtagaagca agttcagtga acggaaatgt caaagtcgct
     3361 ttaccagatg gcatcggttt ggaaggacat gctaaaacaa gtcttggaag tatcaacagc
     3421 cgtctgtcca attacgaagt agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa
     3481 ttccgtcgtg tttcagatgg tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt
     3541 atctatttaa aagatacaga taaataaaga ggttgtgaaa gagatgttca gccttgagca
     3601 ataagaagcg attttgtaaa atagcttccc ctattttaca aaattgtgcc ttattgccga
     3661 tagtctatct cttgaacacc gtttaccaag agattatgaa cctgccaatt atcgccgagt
     3721 cacaaggaat aatttcagaa aaagctcgtc acgttttatg aatatggtaa tttgtgaccg
     3781 agaattttgc agaaagacac cgattaagaa gaaggttgtc acaacgattt tcttcattta
     3841 aaatagtaga acagaggtga gacatgatga aaaaaaagtt gacaaaatct aataaaaatg
     3901 ttgttttgac agggacttta gcaggaatag gagaatacat cgggattgat ccgactgtta
     3961 tccgtgtgct ttatgtattc ctgagcttag ttgcttttgg aagtccaatt attttataca
     4021 ttttattagc tttgattatc ccatttgcgc gttctgcacg aacaaactat ggacataaca
     4081 atgcatatta tgaaaaaaac aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca
     4141 aagaagcgga aaaaatcgat gatgacgaat ggagtgactt ttaatgtcgt actttcaacg
     4201 tattttagtt aatacgttaa cattcatatc attggctgtt ctttttcctg cgatggtcca
     4261 tgtgagcagt atttggacgg ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt
     4321 tgtcaaaccg attttgacga tactgtccct gccctttaca ttagtcacat tgggattgtt
     4381 tacgtttgtt atcaatgcag cgattcttaa aatgacctcc ttttttgtgg gagcagaaca
     4441 ttttagtttt tcttcattcg gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa
     4501 tgtaattgtc agcgaacata acagtagaaa ataaaagaaa gctgtgccga actatcggcg
     4561 cagttttttt atttagaatc aggaatgcgt tttctattac attggtaatt aaataaaaac
     4621 gtttttttta attggtagta cttttggcta gacaatgata aaatgataga gaacactcta
     4681 atagaaatga ggtagcatga atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat
     4741 tatctttgga aatcgtgaca ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg
     4801 acatctcacg cccaggacta gagttgactg gttattttaa ttattattcc cataatcgtt
     4861 tacagctatt tggcagtaaa gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt
     4921 tgatcatcat gagaagatta tgtagtgaag atatgcccgc atttattatt tcaagaggat
     4981 taccagctcc agaagaaatg atccaggcag caaataaaaa tgggatagct gtactgcgtt
     5041 ctcccatctc aacttctcgt ttattaggag aattatccag ttatttagat ggtcgccttg
     5101 cccctcgaac aagtgtccac ggtgttttag tggatgttta cggattaggt gtactgattc
     5161 aaggtgacag tgggatagga aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc
     5221 gattgatcgc agacgatcga gtagatgttt atcagcaaga tgaattaacg gtgatcggag
     5281 aaccgcctaa aatcctggaa catctgattg aaatccgagg aatcgggatc attgatgtaa
     5341 tgaatctttt tggtgcgagt gcggtacgtg gctctatgca agtccagttg gctgtttatc
     5401 tggaagcatg ggcaaaagat aagaaatacg accgtctggg ctctgaagac acgaccgttg
     5461 aaatcgcgga agtgggcatt ccacaaataa aaatccctgt caaaacagga cgaaacgttg
     5521 caatcatcat tgaagtagcc gcgatgaatt tccgtgcacg tacaatgggc tttgacgcaa
     5581 cgaaaacatt tgaagaaaga ttaagtcgct tgattgaaga aaactcaggc aacgactagc
     5641 aaggagtgac aatatgttag cacaaatcaa tcgagttgct tttgagcttg tagggattcc
     5701 tatctactgg tatgcattga tcatcgtatc aggtatcatt atcgctatgt ggcttagcag
     5761 cagagaggct gttcgggtag gattaaaagc agatgatgtg acagatttta tgttgtgggg
     5821 attgcctatc tccattattg gcgctcgctt atattatgtt ttatttgatc ttccccagta
     5881 tatagctgat ccaatccaaa tattcaatat ccgttcagga ggcttagcta tttatggcgg
     5941 actgattgca ggcgcgatca cgctgtattt ttttactaaa tatcatttta tttctttgtg
     6001 gactttttta gatatcgctg caccaagtgt attattagca caggcaatcg gtcgttgggg
     6061 aaatttcatg aaccacgaag catatggacc agacacgacg cgaagcttct tagaaagtct
     6121 gcatttaccg caattcatca ttgataatat gtatatcgaa gggatctatc gtcaaccaac
     6181 atttctttat gaatcacttt ggagtttgat tggatttatt ttgcttttgt ttttacgaaa
     6241 gaaaccacac cttttgaaaa aaggcgaagt ccttttatgt tatctaatgt ggtatagttt
     6301 cgggcgtttc tttattgaag gattacgtac agacagtctt tactttttag gtgtgatccg
     6361 tatttctcaa ttattatctg ctgtgttatt tgtcggtaca ttgattctat ttatttggcg
     6421 cagaaaaaaa ggaaatttac cggattatga ccgctcatta ggaaaaaatc aacaaattat
     6481 ctgattttta gagcgttaaa aatgaaaaaa atttcaggag tggtaaaatg aattatagag
     6541 tagcggtttt aggaccaggc tcttggggta ctgctttggc acaagtcctc gccgaaaacg
     6601 gacatgacgt tcggatttgg ggacatcgtg cttcacaagt agaagaaatc aacacccaac
     6661 acacgaatca acgttattta ccagatcatt atctacccga ttccatccaa gcattcacca
     6721 atatggaaga tgcagtaaaa gacgcagatg cggtgctatt cgtcattcca acgaaagcaa
     6781 tacgttctgt tgcaaaagaa ctagtgccta aattgaatac aaagccagtg atcatccatg
     6841 caagtaaagg attagaacag ggaacacata aaaggatctc tgacatcttg ttagaggaaa
     6901 tcccagcaga aaaaagaaaa gctgttgttg ttctttctgg accaagccat gcagaagaag
     6961 tagcaaaaca tgacatcact acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat
     7021 atgtacaaga gctgttcatg aataattatt ttcggatcta taccaatcct gatgtgatcg
     7081 gtgtagagat gggggcagct ttgaaaaata ttattgctat tggtgctggc gctattcacg
     7141 gcttagggtt tggcgataat gcaaaggcag caatcatgac gcgcggatta gctgagatca
     7201 gccgtttagg tgtagcaatg ggtgccaacc cgctgacctt tatcggatta agcggaatag
     7261 gcgatctagt cgttacttgc acaagtgttc attctaggaa ctggcgcgca ggcaacttgt
     7321 taggacaagg gcagccccta gaagaggtgc tagccaatat gggaatggtc gttgaaggtg
     7381 tgaatactac ccaagcagcg atggaactag cccaaatgat ggaaatcgaa atgcccatca
     7441 ctcaaacaat ctatgaagtg ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa
     7501 tcatgttacg tgatggaaaa aaagaaaatg aattctcatt aacaattttt tgaaggagcg
     7561 aatgtatatg aaagttaaaa aagcagttat tccagcagca ggattaggaa caagattttt
     7621 accagcaaca aaagctatgg caaaggaaat gttgccaatt gttgataaac caacaatcca
     7681 gttcatcgtt gaagaagcgt tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa
     7741 agctaaacga ccaatcgaag atcactttga ttcaaatatt gaattagaaa caaacctaag
     7801 tgaaaaagga aagaccgaat tattgaaatt agtagaagaa acgacagatg tcaacttgca
     7861 tttcatccgt caatctcatc caaagggttt aggacatgcc gttcttcaag caagagcctt
     7921 tattggaaat gaaccatttg tcgttatgct aggcgatgat ataatggaag acgaagtacc
     7981 attgacacag caattaatcg atgattacga acgtactcat gcttctacga tcgccgttat
     8041 gcaagttcct catgatgaaa cttcaaaata cggaattatc aatccaggag aagtattgga
     8101 agatgggtta tacaatgtca aaaactttgt tgaaaaacca gatccaagca aagcaccaag
     8161 tgatttagct attattggac gttacttatt gactccagaa atttttgatg tattgtccga
     8221 gcaacaacca ggagccggaa atgaaatcca actaactgac gcaatcgata cattgaataa
     8281 aacacaacgc gtatttgccc gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt
     8341 tggttttatg aaaacaagta ttgaatatgg tctaacacat ccagaagtag gtgcaccttt
     8401 acgtgagtac atcctgtctt taggtaaaac attagaagca gaaggaagca agatcgatca
     8461 taatccaaaa ggaataacag aaatcaaaga agaaaagaaa aaataaccct tttttctaac
     8521 gagcttcagc tgctaattga ataaaaagta gttgaagctc gtttttattt gtttaaaaaa
     8581 cacgaaaatg ctatccttat agaaaagaga gacaagaagg gagtttgatc atgtcaggag
     8641 gagaaattgc aggattgatc gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc
     8701 gtgtgttgtt gcaagtttct aaaactttag aaaaagtaga tcagactgta gcagaagcaa
     8761 atacgacgat cgaaatcgtc acaaaagacg tagatctttt gtctagacaa gtagaaggct
     8821 tactggtcaa aagtaatgaa ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc
     8881 cattgttcac agcagttgct gatttaagtg aaagtgtatc agaacttaat cattctagta
     8941 aaaacatcgc gacgaaagtc ggcggaatcg gtaaaacgac agctaaagcg acggttgccg
     9001 gaaaagtcgg tgggacagcg atgaaatttt ttaaaaacaa aaaacatact gaaacgacag
     9061 gaggaaaata aaaatggcta aaaaaggtgg atttttatta ggtgcagtag tcggaggcac
     9121 agccgcagca atcgccgcgt tattgttagc accaaaatct ggtaaagaac ttcgggatga
     9181 attagctgcc caagcggatg atttgatgga tatggcttca gattacacag atatggcgaa
     9241 agaaaaatcg actgagttaa caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca
     9301 agcaggtgat ctagcaggta gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg
     9361 aagtgaatct gacgatatct tgttaaatct taaagaacaa tcagctgact tatctaatcg
     9421 tttcaaaaaa acggcagaag atatgaaaaa acctgcagat gaattaggaa gtattgctaa
     9481 agatacttca gaagatatca tgattgatgt aaaagacgct gctggtaaag caggagatac
     9541 tgtcaatgaa ggaatgtcag aagcaaaacc agttgcagat gactcaaaag aaatgatgaa
     9601 aatggcaaaa gaagatgcga aagaagctaa aaaagaaata aaagatacaa tgacagattc
     9661 atctacaaat cactaaaatc acaaatttat agaataaaaa ggtgggagcg gactagctct
     9721 cacctttttt actcttattt ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc
     9781 acacagccat cgcttgtcac atatacacag tcttcgatac gtacccctac tttttcagga
     9841 atatagattc ctggttcaag agagaagcac atcccttctt cgattactaa gtcatttcct
     9901 tctacaaggg aagggaattc gtggacggtt gttccgatcc cgtgacctaa acggtgattg
     9961 aaatattcgc cgtagccata gctgttgatc acgtttcgtg cgatttgatc aagttcacct
    10021 gctgtcacac ctggacggac ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg
    10081 tagatttttt cttggaagtc ggttggtttt tgataagcaa ttgtacgagt agcatcactg
    10141 caatagccat tccaaacaac gccaagatcg aataacacaa gttcattcgg tgaaatcttc
    10201 gtatttcctg gtgtaccatg agggcttgca gcatttgctc ctgcaaggac taatgtgtcg
    10261 aaagacattt gtgaaacacc ttgttttttc aattggtatt caatttctgc aatgatcgct
    10321 tgttcagtca cgccttcttt cactgcttta aagccaatct caaatgcaac atcggcccat
    10381 ttaccagctt cgatcagttt gtctttttcc tctagagttt tgatcagttg tagacgttgg
    10441 atgatggaag ttagatcagt gtaagaatcc gttcccgtca agatatcaga gatagcatcg
    10501 aagcgctcga cagtcaaggc gttcttttca atccctacat tgccaggctg accatagcga
    10561 gcacgaagtt cttttccgat gatctcccaa gggttttcgc tgtctaaata gccacggaca
    10621 tcatacgccc aactactgtt ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa
    10681 ggatcttgtt caagagggat aaaaagagct agtacacgct catgaggatc actttcgtat
    10741 ccggaaaagt aagcgatgtg gccagggtcg ctgatataag ccaattcgac ttggtgttgt
    10801 gccatccatt gacgtagttc attgattttt acgttattca tttggcaacc tactttctaa
    10861 attaattttg tattcaataa ttgtaccact gatttattaa aagaccaacg ttctaaaaac
    10921 tgaaaaaagt aaaaattttc atgaaaacag agataaaacg gttgcaattc ttgagattat
    10981 ttgatattct attttagaaa tgtaagacgt attttcagaa aacatataaa atatgaatag
    11041 gagaacacaa atggaaaagc aaacaatcac tatctatgat gtagcgagag aggcaaatgt
    11101 ttcgatggct actgtttcac gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag
    11161 aaaaaaagtc ttggaagtta tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg
    11221 cttggcaagt aagaaaacaa caacagttgg tgtaatcatc cctgatgtca gcaatatgtt
    11281 ctttgcttct ttagcacgtg gtatcgatga tgtggcaaca atgtacaaat acaatattat
    11341 cttagcaaat tcagatggca acgaccaaaa agaagtcaat gtattgaata acttactggc
    11401 taagcaagtg gacggcgtga tcttcatggg tcaccatatc acagacgaaa tccgtggaga
    11461 attttcacgt tcaaaaactc ctgttgtatt agctggctct attgatccag atgaacaagt
    11521 aggtagcgta aacattgatt atgtatccgc aacaaaagat gcagtaaaca aattggcaaa
    11581 aagcggcaat aaaaaaatcg cttttgtcag tggtgcgttg atcgatccca tcaatggaca
    11641 aaatcgtctg aaaggctata aagaagcatt aagcgaaaat aacctaccat atagcgaagg
    11701 attgatcttt gaagcacaat acaacttcaa agatggtcta tctctagtcg atcggatcca
    11761 taatagcggt gcaacagctg tctatgtttc agatgatgaa ttagccatcg gtattttgga
    11821 tggtttatta gaccgtggcg tgaaagtacc ggaagaattc gaaatcatca caagtaacaa
    11881 ttcactactg acagaagtag ctcgtcctcg tttaacaagt atcacacaac cattatacga
    11941 tttaggggcg gtatctatgc gcctattgac gaaaatgatg aataaagaag aagtcgaaga
    12001 aaaaacgatt atcttgcctt atggaatcga agaaaaagga tctacaaaat aataaaagga
    12061 caaccattca gaggttgtgt gcctgccgtt taactctgcg ttacaaggag caatttcaaa
    12121 aaacagcatc tcatgtttga tgaatattgt gactcgtgat tcggagttgg caggagaaca
    12181 ccgtttattt gggaaaaggg gttatgacaa aattttgtta taatcctttt tttgtgtctt
    12241 aaaaaagaga gaaagtggtt gaaattacat ttatcgtata taaaacaatc cgtgtataga
    12301 tgctgcattt tgttcaccac catcaaaaaa ataagggact gtgacaatac tattgtcaca
    12361 atcccttatt actgtgtatt ctattaaatc aatcatcttt ctcatctgat tctttagttg
    12421 cttgattcgc tcttgcataa gctgaagcgg tgttccagta atctgtatag ttttcatcgg
    12481 taccgccaac gccaaattta aaggcgcttt tcttcggtcc atttttcgcc catagcgatt
    12541 caactgtttt tcctggtgtt tggatggatt ttttattgac agtaactttt ccaggtgttt
    12601 gaccagtaaa tccagatact tttttcttca tcacatcaga tgataattcg aatttctcat
    12661 cagtgccaaa aatgtcaggt tttgcttggt aagcttgatt gatcagattg gcaagataat
    12721 tagccgaacg tatccttgct ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc
    12781 tactgatggt aatagacgga gtagagacaa ttagccatga atctcggtat tcattggtag
    12841 aaccagtttt tccgacccaa tctgcttttc ctagatttcc gtttaagctt gagatggcat
    12901 ctttgaatgg tgtagtgatt tttgcattga tcacactacg catcatgtca ttcatgatcg
    12961 atgccgttgc ttccgaatag atccggacag agttgctttc gtgtttataa attacatttc
    13021 ctgcgttatc tgtgatggaa tcgatgatat agccttcttc atagacgccg ccgtttgcta
    13081 aagcctgaaa tccgttggtc tgttgaagcg tcgtgacatc tactgtacct aaaggagcag
    13141 attcaacgcc ccaattgtca tttgccgggt agttcatctt tgccaagtag tgctcgtaag
    13201 catattgttt cgatccaccg gaattcaaca catcttgata taagtgataa gccgggatat
    13261 tgttggacca ttctagtgac tcacggacgg tttggaaggt gttcgatcct tcgttagtag
    13321 cattgacgat tttctcgcca gcattttctc cttcttgcca agttgttgca taatctgata
    13381 cacgggattc tgatccgata agccccatgt cgattgcagg tccataaaca agaactggtt
    13441 tgattgatga gccagcttga cgttctgtat cgaaggcatg gttattttga ttctgactgt
    13501 aattccgtcc gccaacgaat ccgtagatgc ggccagttct gttatccatc aatacatttc
    13561 cggtttctac ttgactgcca gcaccatcat ctaacatgta tccataattt tcaacgccat
    13621 tttgcaatgc agcatagaca tctttatcga ttgtcgaatg gattgtatat cctttatttt
    13681 ggatcgtttg ctgtgccttt tgataataag cgtcactgac cgatgaatca gacatatcag
    13741 cgttgtcttt ttcagccaat tgttgtgcaa tgattcttgt tgcttcattc atcacagtat
    13801 aatacaagaa ttctctgtca gtctgttctg cgatttgttg tggtagaaag tcttttgtaa
    13861 ggtcataggc ttttgcttct tcgtattgtt ctttcgtaat ttggccttca cgatacatac
    13921 tgaataaaac aaaatctttt ctggcaaggc cggcagaaag atcttctttt agcgcgcctg
    13981 tgttcgtgta tggactatac gtgatcgggc tttggggtaa tccagcgata taagcagctt
    14041 gcggcagcgt cacatctttt gcatctacac caaagattcc ttttgcagct tcctggactc
    14101 cagcaatgtt ttgtccctta ttattacgac caaatggtga gacattcaaa tacgtagcaa
    14161 tgatctcatc ttttgaaaag tatttttcta cttgtgcaga aagaatgatc tcgtttgctt
    14221 tacgtttgaa tgttgtttca tctgtaagga tctgctgttt gactaattgc tgtgtcaaag
    14281 ttgatccacc agaagagcca ataccagtag cttcagatac taatgctcgt aaaacagctt
    14341 taggaacgta accttggtgt ttatcgaagt attcatcctc tgtgctgata attgcagttt
    14401 tcaataaagg tgaaatctta tcagatgaaa tagtcgtgcg catcagatct gtctggattt
    14461 ttgatatctc tgtattatct gcataaacaa tctttgacgt ttctgtaatg tttcctagtt
    14521 ctgtctgcag tgctttttta gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc
    14581 cagcgcctat tccaatacct agagctgcga acatcaaaag aagaacagta ataaaaacag
    14641 tcaatgattg aaaaactcta aaaataatct taggaacaaa ccatttttct ttttttcttc
    14701 tattctgttt ttttcgagta tttttgcgtg gcaaaagggt cacctca
//
LOCUS       DAYYCW010000071        14595 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000071 DAYYCW010000000
VERSION     DAYYCW010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.073"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1718
                     /gene="yfcC"
                     /locus_tag="ACHAYA_001978"
     CDS             <1..1718
                     /gene="yfcC"
                     /locus_tag="ACHAYA_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM8605768.1"
                     /translation="KPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGKFSTQDIEY
                     KDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPEKGLEKVIA
                     DGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGGFGFLNFVF
                     EGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLERLALPLLF
                     FAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNATSWMSPFS
                     VAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLTYKSDDYFR
                     SHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFFVMGLVAGI
                     IAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASVPSALNTIL
                     HGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADLVGVSRQVA
                     VLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLGTIFIMIAI
                     AIGLQ"
     gene            1875..3059
                     /gene="iadA"
                     /locus_tag="ACHAYA_001979"
     CDS             1875..3059
                     /gene="iadA"
                     /locus_tag="ACHAYA_001979"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM8605769.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3170..3373
                     /locus_tag="ACHAYA_001980"
     CDS             3170..3373
                     /locus_tag="ACHAYA_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8605770.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3474..4136)
                     /locus_tag="ACHAYA_001981"
     CDS             complement(3474..4136)
                     /locus_tag="ACHAYA_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8605771.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4149..5651)
                     /locus_tag="ACHAYA_001982"
     CDS             complement(4149..5651)
                     /locus_tag="ACHAYA_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8605772.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6199..7878
                     /locus_tag="ACHAYA_001983"
     CDS             6199..7878
                     /locus_tag="ACHAYA_001983"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8605773.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8005..8484
                     /locus_tag="ACHAYA_001984"
     CDS             8005..8484
                     /locus_tag="ACHAYA_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM8605774.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8703..9383
                     /locus_tag="ACHAYA_001985"
     CDS             8703..9383
                     /locus_tag="ACHAYA_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM8605775.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9391..10722
                     /locus_tag="ACHAYA_001986"
     CDS             9391..10722
                     /locus_tag="ACHAYA_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM8605776.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10737..11699
                     /locus_tag="ACHAYA_001987"
     CDS             10737..11699
                     /locus_tag="ACHAYA_001987"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM8605777.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11810..12148
                     /locus_tag="ACHAYA_001988"
     CDS             11810..12148
                     /locus_tag="ACHAYA_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM8605778.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12227..12934
                     /locus_tag="ACHAYA_001989"
     CDS             12227..12934
                     /locus_tag="ACHAYA_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM8605779.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13037..14518)
                     /locus_tag="ACHAYA_001990"
     CDS             complement(13037..14518)
                     /locus_tag="ACHAYA_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8605780.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4621 a         2524 c         3147 g         4303 t
ORIGIN      
        1 gaaaaccgaa acgcgacttc actaaattat cgacacccca tacgtatgtc attatttttg
       61 gtgtagtcat ctttgcatgg atactgactt ttgtcgttcc agcaggaaag ttcagcacgc
      121 aagatattga atacaaagat gcaaatggcg aaacaagtac tcgtactgtt ttacgtcaag
      181 attccttccg ttatgcttac gagctggata agtcgtatgt gtttgatcaa ttagaagaac
      241 tgcaagatca tccagcagaa cgtgaaaaac tggatgttcc ggaaaaagga ttggaaaaag
      301 tcattgctga cggagaaaaa aatcttacgc aggaaaaact agatgaaatc tccttgacag
      361 atgatgtgct ttacgatcag tacggcgaaa atatctatga tacttctaaa aaattgcata
      421 aaacagccaa aatatgggga acagatgatt ttggcggctt tggtttctta aattttgttt
      481 ttgaaggact agtatctgga gataagtatg gatctgctgt agggatcgct gcattgatcc
      541 ttgttgttgg aggtgctttt ggtattatta tgcgtaccgg agcgatcgac gcaggtatct
      601 atgcgtttat cagtaaaact aagggactgg agcgtttagc tttaccgcta ttattctttg
      661 ctttttcatt cggtggcgct acattcggga tggcagaaga ggtaattcca ttctcaatgg
      721 tgatggttcc atttgtcatt gcgttaggat atgactcgat cgtagcagta accgtgactt
      781 atgtcgcatc tcaagtcgga aatgcgacct cttggatgag cccgtttagc gtagcggtgg
      841 ctcaagggat tgctggaatc cccgtgttgt ctggtgcgac attccgattg attatgtggg
      901 tagttgttac tgcattagcc gctggttata tgatgatata tgcagaaaaa atccgtaaaa
      961 aaccagaaag ttcgttgact tacaaatcag atgattattt ccgttctcat atcaaaaaga
     1021 catctgatga aaataaaaaa ttcatgcttg ggcataaatt gatcttatta gagatgttag
     1081 ttgtccttgt ctggatcgtt tggggcgtga ctcaaaaagg ctattacatt ccagaaatcg
     1141 cttctcaatt ctttgtgatg ggattagttg ctggaatcat tgctgttctt ttcaaattag
     1201 atggaatgaa agtcaatgac atcgcttctt ctttccaatc tggtgcagct gatttagctg
     1261 gtacagcaat cgtagtaggt atggccaaag ggattcttct tgtcttggga ggatctgatg
     1321 catctgttcc ttcagctttg aatacgattt tgcatgggat cgggacggcg ttgaccggtg
     1381 ttccagctgt cattggtgct tgggcgatgt acatcttcca aagtttgttc aacttggttg
     1441 ttacatcaaa ttcaggacaa gcagctttga ctatgccaat catggctcca ctggcagatt
     1501 tagtaggtgt atccagacaa gtagcagttc ttgcttatca gcttggttca ggttttatgg
     1561 acgcttttac acccgtatca gccagcttga ttggtgtgct aggtgttgca cggatcgaat
     1621 gggcaaaatg ggcaagattc caaatcaaga tgcaaggatt cctgtttgtt ttaggaacta
     1681 ttttcatcat gattgccatt gcaataggtc tgcagtagta ctggagacct gtaaataaaa
     1741 aagtaatcca aacagtagat aaggactcca agaaccgtca ttttacggta aaatcaaagg
     1801 agtaaagatc ctatcgtttg gattattttt taagatccta tcatcagtta tacttaaaga
     1861 aagaggaaaa atgtatgaaa atcattcgac aaattgaagt gtttgcacca gattacttag
     1921 gaaaaatgga tgtactgatt gccggtggga agattttggc tgtagaagaa cagctagaag
     1981 gcggctacga aggagtggaa gtagaagaac tttcaggtga gggaaaagta ctaaccccgg
     2041 gtttcattga ctgtcatttc catattttag gtggaggagg agaaggcggt tatcaaaatc
     2101 gaacgccaga agtcacattg agccaattga ctacagctgg tgtaacgaca gttgtcggtt
     2161 gtttgggaac agacggagaa ggtagagata tgactgcgtt gatcagcaaa gcgaaaggtc
     2221 tggaagcaga aggaatctcc acttatgtat acgaaggttc gtaccgattg ccggtaaaaa
     2281 cagtaacaga ttctatcatc aaagactttt tgacaattga taaaattatc ggaatcggag
     2341 aaatcgcagt atccgatcac cgttcttctc agccaagttt tgaagagttc gctcgtgcag
     2401 tagcagatgc tcgtgtagga ggcatgttat ccggaaaagc tgggatcatc aatgtccact
     2461 taggcggagg aaaaagaaag ctagagctat tgacacgaat cgtagaagaa acagaaattc
     2521 cgatcacaca atttttgcct actcatgcaa atcgcacacc agaattattt gaagcttgcg
     2581 tggcatttgc caaacgtggt ggcacgattg acttcactgc tagcgaagat cctgattttt
     2641 gggagaagac agatggtgaa gttcgcttca gcaaagcact gaaacgattg atcgaggaag
     2701 atgtttcttt agataatttc acgatgacct cagatggaca aggaagcttg ccttattttg
     2761 atgaaaacaa tcatttccta gggttaggag taggaagtgc caaggcattg ctagtaggga
     2821 tcaaagaagc tgtgcaaaaa gaaagtatcc ctttggaaat cgccttacga gctataacca
     2881 gtaatccagc acgtatattg aaattggata aaaaaggaaa aatcgaaatt ggtgctgatg
     2941 cagatctgtg tatcttggat aaagaaacgc ttgatattga tacagttatc gctaaaggag
     3001 aaataatggt ccaagaaaaa gaagtcaaag tatgggggac tttcgaaaaa agtttttaat
     3061 caacagctct aactatcttt tttagaaaac gagcgttctg tttgtctgta tctgttgttg
     3121 atgttaccct gaaggtagat caataaacaa actaagagga ggaagcacaa tggcagattt
     3181 aaaaggacgt tctaaagata tgaaagataa agtagtcggt gaaggaaaag aagcatacgg
     3241 caaagcaaca gacgacaaga gcaaagaaac agaagggaaa gcacaatcac taggtgctga
     3301 catcaaagaa aaagctcgaa atttaggcga cgaagtcaaa gacaagtttt ctcgaaaaga
     3361 cgaaaaaaaa taaaaaccac ttattactaa ttgaatcaag caaataaaaa atccgaacca
     3421 tatctgcgag acagatatgg ttcggatttt ttatttgatc attatcctgt tttttaaact
     3481 tcgatttttt gaaatttttt attttttagt tcaaagactt catcacagct ttttgctact
     3541 tcaatctcat gagtcacgat gataatacag cgattttgtt catgggcgat ttcttggaaa
     3601 agtttgacga tatcttgtga agttgtttca tctagatttc cagtaggctc atccgctacg
     3661 attagttgat gttcacagca gatagcacga acgatagcga ctcgctgctg ctgacctccc
     3721 gaaagtttgg tcacctgttt ttttgctaat atttcatcaa ttcctacacg agccagtcga
     3781 tctaaagcaa cttgtcgata attctcttgc ttggtcttag tgatttccat tgctgctatg
     3841 acgttgtcta gagccgataa ataaggaagc aagttgtaag cttggaaaat aatcgagaca
     3901 tcatttttac gataacttct taacccgatt ttgttcagtg aacgatcttg atagtatacc
     3961 tttccttctt ttggtgcatc tagtccagaa ataagcgaaa gaaaagtcgt ttttccagaa
     4021 ccacttgctc ctaaaatagc gtacattttt ccagcttcga aattcagttc tacatcttta
     4081 taaagcgcat cttgcggatc ttgataccaa aatcccaatt gttctgtccg taacatcaaa
     4141 ttcctccttt agtttaatag gatttttctt gggtttaatc ttaatatccc tatagaagct
     4201 aaaaagacag aaaacagact gattccaaat gctactccgg ctaatagtcc caattgtgca
     4261 ggtttgacat tgatttccaa ttcgctgact tgatcagaaa cagcaaatgg attattcgtg
     4321 gttccttgtt gaccatcgcg ttgaccgcct tgttcttgtc tgttacctgg catttgtcca
     4381 ccgccaggag cttgctggtt ttgtgtcgtg gtttctgtct gttggtcaag tagctgctgt
     4441 cccacggcat tcgcgacgac gtttccgcta agcgaagcaa ctcctaatgc aagaatcata
     4501 caaatagcca cttcggtaaa caattggaga atgattttac ttctggattc tcctaaagat
     4561 agcaagacgc ctaactcatg ccgacgctcc cgaatggtaa tcataatgat cagagtaagt
     4621 atgataatac cggctacagc cactagtaaa acaacatttt tagaaaaact tgccacgttg
     4681 tttaaaggtt ccaacattga ctgatacatg gaatcatttg attgtaagct aaatgtatct
     4741 gtgtcgatca gcttctcagc ttctttgaga aactcatcca ttttatctgg atcagataga
     4801 gtgtaaatag cagaatctat cgtgttatcg tcagatgtcc catttagttc atttgcaaaa
     4861 gtgtaagaag tgtaaagtgt attcgacgga ttcatgaagt tgaatttcat acccatactg
     4921 ccgcttgttt cgctggattc ataaattcct ttgatcgtca tttcataagt ggtatcttca
     4981 tcttcagtac tcgtgattgt gaaagtatct ccgactgcca ggtcatttgt ttctgctaga
     5041 gtagaatcaa tgactacatt gtttgtgcct ttgtcgctgt catctattcc ttcaccatcc
     5101 gtgatttttg ctgttccatc agaaaaatta ctattttgtg cagttgctga cacaccactg
     5161 atttgaaagt ctgcttgagt catttgcgga ttcccgccca ttcctccagc aggttcaccg
     5221 cctgcatcag tagtttctga actgtcttca cttgaatcag aactagtgat ggcagttatt
     5281 ccatcttttg ccatagctga ggcggaactt tcaaaggaat aactctttac attatctaat
     5341 tcagctattt tttccgcgtc cgttaaggaa actggtgtaa gagaaaaact gcctggatct
     5401 ggacgttctt cagtggtaga accgcttgaa gaagtttgtt cctgtttttt aaatgaagct
     5461 tctcgattgg cacttaaagt cactgttgct ccaacttctt tttgtgcttg attcgctgct
     5521 tcttctgcag cgcttcgaat cgtgaggcca gctagcacaa aaataagaat ggctgtgaac
     5581 acggcaaaca ataacaatga acgtccccat tttacttttg tacttttcca tgcacgtttc
     5641 ataaaattca tattttgcat ccctcctagt agatgcattt gataatagac aacctatctt
     5701 aaagctagct taaatcttat gctgtttgca caccatcaac atgaggttct gtatatataa
     5761 aaagtcacta tagttattgc aaaaaatgtg gtaaaatgaa aaaagtatgt ttcaaaccga
     5821 cgaagaagaa atgaaagagg aatagcaaaa gaaatcctag atgctacaga gggattttta
     5881 cgtgcatagt tgttctaaca attgctgata ctagcgattt agtttatttg ttgtataata
     5941 atggggaaca gctgaatact tatataagta ttctggctat agcgtttcgc aaaagatcca
     6001 ttttgttaat gaagtatatc caaaataaaa tgcccatcca actgtcagaa cgcggatgtg
     6061 ctggtaattt tcctgctgat tctccataag ttgagaaata gtgattttgc atatgcttca
     6121 ttggaatggc tcatatattt agataaagaa gaaaatcgac atctttaaac ttggatgtca
     6181 taagaagagg tgaagtttgt gagtaccatt aaaattatcc cattaggcgg cgttcgtgaa
     6241 aatggaaaaa acatgtatgt cgcagaagtg ggcgaggata tttttatttt agattgtggg
     6301 ctaaaatatc ctgaaaatga gttgttgggg atcgacgtcg taatccctga ttttacgtat
     6361 ttagaagaaa acattgatcg tgtagctggt gtctttttaa cacatggaca tgcagatgcg
     6421 atcggggcac ttccttactt gttgtcaaaa atttctgtac cagtattcgg gacagagttg
     6481 acgatcgagt tagcaaaatt gaatgtcagt cgtaatgaag cagccaaagg atttaaggat
     6541 ttccatgtgg tagatgagca tacagaaata gactttggtg aaacagtagt gagctttttc
     6601 cgtaccactc atacgatacc agattcagtg gggatcaatc tgaaaacaaa agaaggtagt
     6661 atcgtttata cgggagattt taaatttgac caaacggcta tcccaatgta tcaaacagat
     6721 tatgcacgat tggcggaaat cggcaaagaa ggagtcttag ccttactaag tgaatcggca
     6781 aatgcagaaa atcctcagcc tgtagcttct gaagcccaaa tagcagatga agtatttgat
     6841 acgatcaaat attgggaagg gcgtatcatc gtcgcttgtg tagcaagtaa tttgcaacga
     6901 gtgcaacaag tattggatgc agcagaaaaa gcacaacgaa aagttgtctt gactggtcaa
     6961 gatttcgaac gaattattcg cacagcgatg cgtttagaaa aacttcaatt accaagtgaa
     7021 gatctgcttg ttacgttgaa agacatgaaa aactatgatc cagaagaact gatcatttta
     7081 gaaactggcc gaatgggtga acccatcaag tctctgcaaa aaatggcaaa cggtacacac
     7141 cgtaatgtca agattgaaga aggagatttg gtttatatca ctactacgcc aagcatcgcg
     7201 atggaaacca tcgtggcgaa aacggaagat atcatttatc gagctggtgg aacagtgaag
     7261 ttgatctctg agaatatgcg cgtttctggc catgccaatc ctaatgactt gcaattaatg
     7321 atcaatttga tgaaaccaac atattttgtg ccagttcaag gagagtatcg tgaattagct
     7381 gctcatgcag atttggctca tgcagtaggg atgccataca aaaatatcta tattactgga
     7441 cgcggagacg tgctggaata taagaacaag cgtatgagtg ttgcaggaac tacaagtgca
     7501 gaaaatgtaa tgattgatgg attaggcata ggtgatatcg gaaacattgt tttacgtgat
     7561 cgaaaaatct tgtcagaaga cgggattttc gtagcggttg tcactatcag tcggcgagaa
     7621 aagaaaatca tttcaaaacc tcaaattact tcacggggat tcgtttacgt aaaagctagc
     7681 cgtgatctga tgaaagaaag cagcaatatg attgaaacaa tcgttgagaa acatttagaa
     7741 agcaatgatt ttgagtggag caaattaaag caagacatcc gagatcaatt aggccgttac
     7801 ttatttgaac aaacaaaacg ccgcccagtg atcttgccag tcattatgga agctactcaa
     7861 cgtcgaaata aaaaataaac aaaaagagta agatgaacct ctctatagag tgctcgtctt
     7921 gctctttttt tgcgaaaaac aaataatttt tgaatacagc atccctcgct ttataataaa
     7981 cgatagaata aagggagtga atcaatgatg agtcaaacaa aagtcgaacg ttatttagaa
     8041 gaacaaaaga tcagctatca accacttgat tttagtcatg tggtagaaga atctttcaca
     8101 gaagaactac aaaaaagagg gattgatcca gctttgatat gtaaaactct cgtagtgaaa
     8161 ggaaacaaaa ctggggtgat catcgcattg attcctttga acgatcactt agattacaaa
     8221 aaaacgagac aagtgacgaa agatcgtaaa gttggttttc ctggaatgga ttttgtatta
     8281 atgcatacag gatatcccca cggagcaaac acccctattg gaataaaact cgcacatcct
     8341 gattattgtt ttttagcaga cagttcttta aaagaaaaag aagaagtaat tgtgtcaagc
     8401 ggtgaaatcg ggagaagcct tcggataaaa gtagccgatt tagaaaagat cgtccatcct
     8461 atttacgctg atttaattag ttaagggacg agaagagaag ctaagcagag cagagacctc
     8521 aaacttgaga ttatatctgc ttggtacttg gtgcatcctg tattatctgt tacaattgct
     8581 ttgtacatac tgtactatca atccaatgaa aatatattta gcagctatat tttttcattg
     8641 atgtgcaagt gatgaaatcg tttttaataa aaaagataca gagaaaacgc gaggtgtaaa
     8701 atatgaaaat tacgtatcat ggacattcct gtttaacaat tgaaatggaa aatggccaac
     8761 gcgtgatgat tgacccgttt attacaggaa atccattgac agacttaact gcagaagaag
     8821 taaagacaga ttggttgctg attactcatg gtcatagtga ccacatagga gatatgattc
     8881 ctattgcaaa agaaaatcaa gccacaatta tcagtattgt tgagattgct gattatgcga
     8941 acagcagagg agtagattca ttcggtatga atatcggtgg aaaacatgcg tttccattcg
     9001 gcgtggtaaa attcgtacat gcacagcata gttcaagcta tgaagtagac ggtatcgttc
     9061 agtatatggg cgagcctagc ggaattatta ttcaagcaga aggaaaaacg atttatcatg
     9121 caggggatac tgcttatttc agtgatctag gattgcttgc agaagaattc gatattgatg
     9181 tcgctttctt gccgattggc gataactata cgatggggcc agaagatgca gcaaaggctg
     9241 cgcaaacaat tcgtgccaag aaagtagttc caatccatta caatactttc cctgtcattg
     9301 aacaagatcc cgaatctttt gtagagttac tggaagatag tgaaggaaaa ataatgagcg
     9361 tctcggaaac gctggaagtg taggcagaaa atggcgacaa aacatgattt gattttagaa
     9421 tatattgaaa gtttacctgt tggcaatcga atttctgtaa gaagcatcgc aaaagaattg
     9481 aatgtcagtg aaggaacagc gtaccgtgca atcaaagatg cagaaaatgt cggactagtt
     9541 tcaacaattc aacgagtagg gaccatccgt atcgaacgca aattaaaaaa acatattgaa
     9601 cgtttgacat ttggcgaggt cgtcaggatc attgaagggg acgttttagg cggggcggca
     9661 ggattagaca aggtcttgaa taaattcgtc atcggcgcga tgactaaaca tgccatggaa
     9721 cgctatatta cgccaggatc actaatgatc gtcggcaacc gggcggaagt ccaaaaatta
     9781 gcattggaag atggcgccgc agtattgatc acaggaggat tcaatacgac tgctgagatt
     9841 gcaaagctag ctgatgatct tgaaatgccg atcttacgca cctcttatga tacatttaca
     9901 gtcgcaacca tgatcaaccg agcgttaagc gaccaattga tcaaaaaaga tatcatgctt
     9961 gtcagtgata tctatatgcc agctgaaaag actcattatt tgcagcagac agatacgatt
    10021 tctgattatc agcgtttgtc agaagaaacg cagcattcgc gttttccagt cgtcaaccgc
    10081 catcatcgtt tgatggggat tgttacggca aaagatgttt tagggaaatc gccaaaccag
    10141 atcatcgatc gggtgatgac gaaagaacca atcagtgtaa agaaaacaat gagtattgcc
    10201 tctgttagcc atcaaatgat ctgggatggg ctagaagtca tgccggtcgt ttccgatgac
    10261 ttgacattgg aaggcttgat tacccggcaa gatgtaatga aagcgatgca gcttgtccag
    10321 cgacaaccgc agattgcaga tacgatttcg gatcagatat ctggaaatat ccatacgatt
    10381 gatactgacc gtgaaggaaa tcgtttggaa aaccctaagt tcaaattcgt agtagcacca
    10441 caaatggtca atggcgttgg aaccatttct tttggcgtac ttagcgaaat catttctgat
    10501 gtcgcacaaa aaacaatggt gatgaaccaa aaacgaaata tcttgattga acaagtgaac
    10561 ctacattatt tgcggctgat ccaattggaa agcgaattag acattcgtcc acgtgtattg
    10621 gaaatcggca gacgttccgc taaactggat atcgaagtgt ttattgaaaa caccatcgtt
    10681 tccaaagcaa tcgttgtttg tcaagtaatg gaacgttctt agaaagcagg agtgaaatga
    10741 cagtacagca agaaatagta gatacaataa aaaaatacga taaaatcatt atccacaggc
    10801 atttgcgccc tgatcctgat gcattaggat cacaagtagg attagccgaa ctattacggg
    10861 aaagtttccc agaaaaagaa atacgtcaag taggtgaaac gattgaaggg cttcgctttt
    10921 tagcagaaat gcaagaagtt gaagatgtct tctaccaagg agcgctagtc atcgtgactg
    10981 atacagctaa ttcacccaga atcagtgatc aaagatatca gttggcagaa aaactgatca
    11041 aaatcgacca tcatcccaat gatgaccctt atggagatct tgtgtgggta aatacaaaag
    11101 caagtagctg tagtgagatg attgcagaat ttgctttgat gtttcctgaa tctttaaaga
    11161 tgaacgttaa ggctgcacgg ttactttatg caggaatcgt cggagataca gggcgctttt
    11221 tgtatccctc cacgacgtcc cgtacattag agattgcttc gatattgaga aattatccat
    11281 ttgatgcaag tgctttgaac agagagattg aacaaatgcc gatgaaagtc gctaaacttt
    11341 ccggttatct ttatcaaaat atccaggtgg atgaaaatgg tgcaggaaga ataatccttc
    11401 cgcaagaaat tcttgataaa tatggaatcg atgattcaga gacagcttcg atcgtttcac
    11461 ttcctggagt gattgacgaa gtgctggctt gggggatttt tgttcagcag ccttcagggt
    11521 attaccgcgt acgtttacgt tctaaggggc cgatcatcaa tgaattggct aaacgccatc
    11581 atggaggagg ccatccatta gcaagtggtg cgaatgcaaa agatttagca gaagtagagg
    11641 aaatctataa agagatccaa gatatatgcc aatcgtataa gagcagaaaa acagaatgat
    11701 acttcgttcg aaaacgtgcc ttttgtagta aaaatcacaa atgtatcttt tcaagatctt
    11761 ctttttccgt tacaatagtg ctcactaaag attttaaagg agaaagaaga tgacattacc
    11821 aaattgccca gaatgtggct cagagtacac atatgaagat cgaggcttgt tgatttgccc
    11881 agaatgcggg aacgaatgga acaaagaaga acaagaaact gtcatagaag acggattagt
    11941 ggtattagat gcaaatggaa accgtctgtt agagggcgat agcgtgacag tgattaaaga
    12001 cttgaaagta aaaggtgcgt caggtgccat taaacaagga acaaaagtca agaacatccg
    12061 tttagtagaa ggcgatcata atatcgattg caaggtagaa ggatttggac cgatgaaact
    12121 gaaatcggaa tttgtgaaaa agaattaaag aatattcaag gtcttttcac attcttttcc
    12181 tactactaat gataaaataa agtaaacgaa aaggagtgaa acgaaaatgt atggttatgg
    12241 aatgtatggt tttttagatc cgacatttct tttggtcatt cttggattag ttatttccgg
    12301 aattgcgtca gcgtacgtca atagtacttt ccgaaaatac gatcaagtga gaagcaaaaa
    12361 caatgtgact ggtacacaag cagcacaata tatattacaa agtcaacaga taaataatgt
    12421 cggtgttcaa caaattgccg gagatctcac agataactac aattcaggta ataagatgtt
    12481 gagtttgtct caagcaactg cacagtctac ttcagttgct gcgataggtg tagcagctca
    12541 tgaatgcgga catgcggtac aagatgcttc gggttatgtc ccattgaaga ttcgtgcagc
    12601 acttgttcca gcagctaact ttggttcgat gatttcgttc cccttgatta tggtaggtgt
    12661 attgcttagc tggaactcta cgttaatcaa tatcgggatt ttcgcttttt ctttagcatt
    12721 acttttccaa ttggtgacat taccagtcga atttaacgcg tctcggcgag caatccaaat
    12781 tttgagtgaa ggcggactac tgacggaaga agaagtaccg atggcaagac acgtattatt
    12841 tgcagcagca ctgacttatg ttgcagcagc gctttcgaca ttcctgcagc tgttgcgctt
    12901 gatcttattg ttcggcggca accgaagaga ttaaaataaa gaagttgtga aatcagcgct
    12961 ttgatctgag ctaataccga gagttagctg attgaatacc atttatttgg aataagagga
    13021 tgtgacaaga attttgtcac atcctctttg ttttgttctc ataacctttt ttcatataca
    13081 gttcatgaac cgcttttatt aacttgccaa ataacactgt attgtgcgtt gtatccagat
    13141 agaaagtctc cgtttcatct gaaattttct catgctgatc tgtaatgatc aaatcagcat
    13201 gttctgaata tgcggtaatt ttaacagcat ctttgcgaaa taactgctgc aaatggcttt
    13261 gaagatgata atttcctgta aactgtttgg ttatctgcaa atggatgagt aactgctctt
    13321 tcgtttctgt ttgtagtaag tgttggatca attgactaag gtattgactg atttggtttc
    13381 tttccccatt ttttgaaaaa attgtatcga ttttttctct taatttcttt tcctgtataa
    13441 aagaactaga atgattggac gtggcttctg gaaagcaaag tgtagaaaaa ctactgctgg
    13501 attcacccaa cagttcgaaa aaaacaactg ccaacacaag aaaataaaaa taaattggta
    13561 ttttattttc taaatggttg agaggaaact gttgagttaa gtaagtcatc aattgaccga
    13621 cttgtcgaca aataaggttt ttgcttgtgg aaaataaaaa accaatctcc tgtttttgtc
    13681 gaaaagagat ttgatttgga aaaaaacaat agaaaaaata attaaagtag gctctctctg
    13741 tttctgtctc ttcacaaggg cgactcactg aaaaagaagt aaagtattct gaataatcat
    13801 gtgtatgctg caataagtcc atcaattcag tgatttcaga agaagttttt ggaaaatatg
    13861 cctgttgttt gtttcgtaat atgaataaag ttaacagcaa gtagatattt ttttcttgtt
    13921 ttgaaatttt ttctatttgc tcatctgtac tgttcatttt tagtgcattt aattcaagtg
    13981 aggaaaatgg ccaatcgtta cctccaaatg tatctgaaag aaaaaagtaa agatagagac
    14041 ggatagataa ctcatttcct tcaagagtac agcaattttt tacagatagg atccgtaaat
    14101 ggaattttat taaggaatga ttgagctgtt ttgtcagttt ttcgagatag gagggcgata
    14161 tataaagttg ttcaagtaaa tatttttttg gtacagcagt ttgtggataa ttgattagta
    14221 acatcaaata gttaaaacga ttggattctt ttaggtagca taattttaac ttagaaaaaa
    14281 cagctaactt tttagtttga tcggtttctt tggtttggat ctctccttta tgattagaat
    14341 aaataaattc atcaatcaat aaaaattgct tgagtgatac atttattttt cggatcagtt
    14401 ctttgattgt ccgttttgga atatgtaagt cattggataa ttcttgtaaa gaacaattat
    14461 ttttttgaat gaccatttgt agaacaagta actctttttt tcttgcttca attagcataa
    14521 atacctcctt tttattctgt ttttgtatat agttagacaa aaactgtgag tggaaagtca
    14581 tttcagtctg tgtta
//
LOCUS       DAYYCW010000072        14171 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000072 DAYYCW010000000
VERSION     DAYYCW010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.074"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACHAYA_001991"
     CDS             complement(<1..1835)
                     /locus_tag="ACHAYA_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8605781.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACHAYA_001992"
     CDS             complement(1832..2536)
                     /locus_tag="ACHAYA_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8605782.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACHAYA_001993"
     CDS             complement(2548..4857)
                     /locus_tag="ACHAYA_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8605783.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACHAYA_001994"
     CDS             complement(5052..5516)
                     /locus_tag="ACHAYA_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605784.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACHAYA_001995"
     CDS             complement(5516..6142)
                     /locus_tag="ACHAYA_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8605785.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFRKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACHAYA_001996"
     CDS             complement(6149..6514)
                     /locus_tag="ACHAYA_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8605786.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACHAYA_001997"
     CDS             complement(6504..6842)
                     /locus_tag="ACHAYA_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605787.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACHAYA_001998"
     CDS             complement(6832..7158)
                     /locus_tag="ACHAYA_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8605788.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="ACHAYA_001999"
     CDS             complement(7139..7417)
                     /locus_tag="ACHAYA_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8605789.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="ACHAYA_002000"
     CDS             complement(7419..8783)
                     /locus_tag="ACHAYA_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8605790.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACHAYA_002001"
     CDS             complement(8796..9365)
                     /locus_tag="ACHAYA_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFM8605791.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACHAYA_002002"
     CDS             complement(9337..10524)
                     /locus_tag="ACHAYA_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8605792.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACHAYA_002003"
     CDS             complement(10588..12315)
                     /locus_tag="ACHAYA_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8605793.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACHAYA_002004"
     CDS             complement(12312..12764)
                     /locus_tag="ACHAYA_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8605794.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACHAYA_002005"
     CDS             complement(12876..13256)
                     /locus_tag="ACHAYA_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8605795.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACHAYA_002006"
     CDS             complement(13253..13639)
                     /locus_tag="ACHAYA_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFM8605796.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACHAYA_002007"
     CDS             complement(13641..13919)
                     /locus_tag="ACHAYA_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605797.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13982..>14171
                     /locus_tag="ACHAYA_002008"
     CDS             13982..>14171
                     /locus_tag="ACHAYA_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605798.1"
                     /translation="MLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGVSSYSA
                     FDNDRIFGVLVQCINDECN"
BASE COUNT         3917 a         3056 c         2231 g         4967 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttcctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac a
//
LOCUS       DAYYCW010000073        13958 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000073 DAYYCW010000000
VERSION     DAYYCW010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.075"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..303)
                     /locus_tag="ACHAYA_002009"
     CDS             complement(58..303)
                     /locus_tag="ACHAYA_002009"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8605799.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(297..698)
                     /locus_tag="ACHAYA_002010"
     CDS             complement(297..698)
                     /locus_tag="ACHAYA_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8605800.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(885..2693)
                     /gene="uidA"
                     /locus_tag="ACHAYA_002011"
     CDS             complement(885..2693)
                     /gene="uidA"
                     /locus_tag="ACHAYA_002011"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM8605801.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(2716..4482)
                     /locus_tag="ACHAYA_002012"
     CDS             complement(2716..4482)
                     /locus_tag="ACHAYA_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM8605802.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(4495..5289)
                     /locus_tag="ACHAYA_002013"
     CDS             complement(4495..5289)
                     /locus_tag="ACHAYA_002013"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8605803.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(5299..6675)
                     /locus_tag="ACHAYA_002014"
     CDS             complement(5299..6675)
                     /locus_tag="ACHAYA_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8605804.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(6795..7442)
                     /locus_tag="ACHAYA_002015"
     CDS             complement(6795..7442)
                     /locus_tag="ACHAYA_002015"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFM8605805.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(7526..8953)
                     /gene="uxaC"
                     /locus_tag="ACHAYA_002016"
     CDS             complement(7526..8953)
                     /gene="uxaC"
                     /locus_tag="ACHAYA_002016"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8605806.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(9027..10106)
                     /gene="uxuA"
                     /locus_tag="ACHAYA_002017"
     CDS             complement(9027..10106)
                     /gene="uxuA"
                     /locus_tag="ACHAYA_002017"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8605807.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(10109..11740)
                     /locus_tag="ACHAYA_002018"
     CDS             complement(10109..11740)
                     /locus_tag="ACHAYA_002018"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8605808.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            12044..12715
                     /locus_tag="ACHAYA_002019"
     CDS             12044..12715
                     /locus_tag="ACHAYA_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8605809.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(12875..13066)
                     /locus_tag="ACHAYA_002020"
                     /pseudo
     CDS             complement(12875..13066)
                     /locus_tag="ACHAYA_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(13434..13775)
                     /locus_tag="ACHAYA_002021"
     CDS             complement(13434..13775)
                     /locus_tag="ACHAYA_002021"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605810.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
BASE COUNT         4537 a         2721 c         1793 g         4907 t
ORIGIN      
        1 tccttaacta ttagaagatt cttttaaaca gaaaaagtgg aaaggggatt tttttaatta
       61 gttaatttta aattttaaga tcgcttctat taatttaatt tctagctgac ttatcatata
      121 ttcatctaca taggtaacga attgacctac ctcgttaaat ccatttgtca aacataactt
      181 agccatataa gaatgataat gtttgagtat catcttgatc gaaattggat ctccttctag
      241 agcagataag ataatggata gtggaactaa taggtactgc cgctttttag atttatctac
      301 cataggtcat accccatttt caattgttca actgccttct ttcgttgata ccagatacca
      361 cttgtgctca tattgagaat tttaccgatt tcagcatcat taaaacctgc aaaataataa
      421 agtaggataa tctttcttct tatctccgat aactgatcaa ttgctgctga taacttttga
      481 tctgaaatta atatttccat tccaaataag aaatagacat cttgttcatc aaaatgagtt
      541 tgggaatacc tgccttcatt tgaatctgcc aatggttcct cattatcctt tatgttagcg
      601 tttcgttttc gaatagactt agcttcgttt cgtaatactt ttttacagta ggcatcaaat
      661 tggtgttgta tagatatgtc attgtaatgt tgttgcatga taatatgctc ctttttcccc
      721 ctttccacta atacaacaat cagaaagccc ctttttttcg aaaaaactag aaaaaaaaca
      781 taaaaatatt tatcattctt taaacagaaa atctttctat aaaaaacaaa aaaaaacgat
      841 taggctaggc taatcgtttt ttgaacattc ttaaaaaatc caaattattt tttataactg
      901 aaattgggta ttgatgacca tcgtttctgt agatagtaag caatcatttt aggttttcta
      961 tctctagtaa agattccttt cttatttcct tgcactctta aaatcccttg acttgttgca
     1021 aaatctgcaa aattccacac ttgttcacca acaaaatttt cacatgtatc tattacttcg
     1081 tgatttgctt cgtaataagc cacttgatac tcttctgtaa acattgttgg tactgtatca
     1141 tgaaatccag ctactgtatc tgctccatat tctgtaaaca ttattggcgt atcaggacat
     1201 ctttccatcc atccatctag ctcttttttc aacagatatt ttgcatgtgc cagatcccct
     1261 cccgaaacat accaaccata ataacgattt aaacataaaa catcgacaat atctcctacc
     1321 ttacaaacat ctggcgttga ttttagataa gtaacgattg ttacaggtct tttctgcgga
     1381 tcaagttctt tagttaattc aactaaaggt tcaaaatatt ctttagcacc ttcggaatcc
     1441 gaatctggtt catttgcaat agaccacatt acaacacttg ggtggttctt gtcgcgttct
     1501 atcaattcct tgatcaccgt tttatgagaa ttttctgttc gtatctcttt ccacgtatta
     1561 tgtttttcca cattatcatc taataaagtg gccatgaaat ttaaatgtaa ccctactgca
     1621 ggtacttcat caataactac aataccttct cgatctgcta atcgcataat ttcttctgag
     1681 taaggataat gtgatgttct aaaagaatta gcacctatcc atttcattaa attaaaatct
     1741 ttaatattta cagcctcatt caaaccacgt ccattaacat acgaatcctc atgcttacca
     1801 aatcctttaa aataaaaagg tttattatta atcaaaaatt tgccattggt aacctgaaca
     1861 ctgcgaatac caaattcttc tgtataggta tcgatcactt tattatcatc aattaaacta
     1921 attttcatac tatataaata agcgtctaga ggttcccaaa aaattggatt cttaagctga
     1981 atcattcctt tctttccttc agcttcagct acaagttgag cctcttcgtc atatatcgct
     2041 attaagattt ttccggtcca ttctgtatcg atattgaatt ctacacttgc atccaaatca
     2101 gttattcttg tattaatagt aatatcactt atatagtctt tcggagttgt atataactta
     2161 acaggtctat gaatacctgc ataattgaag aaatcaaaat taactaagtt ttcctttttt
     2221 atatccccat tttcctgtac tgtttccttc ataatcccca caggtaaagt actatcgtca
     2281 ataatattgt ttacagcaac tgtaattcga ttattcccgt cttttacaaa ccgattaatt
     2341 tcagactcaa atggtgtaaa cccacctttg tgttgagtaa taaactttcc attcaaaaaa
     2401 acttttgctt catgtgttgc agatccaaaa cgaagaacta atctttcttc taataatctt
     2461 ttaggaagag tcacttcttt ttcataccac acccatccaa catgatttct aatgttctct
     2521 gtttcaaata aatcattata agaagaggga actgaaatcg gataactctc tttttcattt
     2581 attggttcta aagcaagttc ttcctgtaag tcatcatttg tatctaattt aaatctccag
     2641 agaccgctta agtcaattac ttgcctcgtc tctgtcataa tgggatacaa catataaacc
     2701 actcctatct aaatattaaa taaatgtatc ctttaaacca caataagcat ctattggact
     2761 tactccctta aattcagaaa gacccattag tttgtccacc aatatttcta aagtttcttg
     2821 ttttccatca taagtattaa tataagtctt tacttgaggt acatcagcca aatgaaacgg
     2881 acactgtaca gatacaaaaa ttgttggaat ttcatgaatg taaaacggta tatccggagt
     2941 tcctttactt gctggccaga caattcgttg aactgtgcct ggattaacat tagctatatt
     3001 aatgatcaag tcatattttt tagttaaatt tttaattggt ctcttagcag catatattcc
     3061 agctatattt ttaatttttt cttcttgcga taatcctttc aattcttctt caggtgaaat
     3121 gtatttttca acttcaaatc cttttgctga caaaatctgt ttcaataaat caatggcttg
     3181 aggtctattt ccagcaacta cttcaccaaa cccacctttt accccatgta catctactag
     3241 taaaatcctt ttatattttt ctggtacaat aggccaaata tcttgtttat ctttaactaa
     3301 tgtaattgct tggtcagcca cctctttaaa aattgattta ttttcaatta aaccaatttt
     3361 ttccagagct tcttgcttag gtaataaaat ttgttctttt gcttttttgt gtaatcccaa
     3421 tttagctttt aaccctaaaa ttctttttaa ggcatctaat aaccgatcat cagtgataat
     3481 tcctttacga tagccttcca tcatataatt aaagtcttca tctggatcat taaaaaatag
     3541 aaatagatca catccagctg caatagaagt gggcaataat tctgatcgtt tcattgcccc
     3601 agtcattcct aacatatgac tagcatcagt aactactaat ccattaaatc cgagttttcc
     3661 tctaagtaag tcagtgatta attcttttga taaagttgca ggaagtaaat cttcatcttt
     3721 cagatcagga ttaaaatgtt ttgaataagc tggcatatgg atatgacctg ccataattga
     3781 aggtaatcca gcttctatta agcctgagta tacttttcca aaagaattat cccattcttc
     3841 cgtacctaaa ctattaatac taaaagatag atgctgatct cgttcgtcaa taccgtcacc
     3901 agggaaatgt ttagctgcag gcaaaattcc actctcctga atacctttca tataagctaa
     3961 actaagttct aaaactttat cagggttttg tccaaaagat cgactagaaa tgataggatt
     4021 tctccaattt ctattaatat ctacaatagg agcaaaactc cagttacatc caatagccga
     4081 tgcttcaatc ccagaaactc tccctaattc ataagcatat ttcgcatcat ttgttgcacc
     4141 aatttttact tctacaccaa cttcagtacc atctgtacaa gctccgttac cacctgcttc
     4201 cgtattagca gcaattaata gaggaatttt actttctgtt tgtaagatat agttttgatc
     4261 atatacctca tcagcagttc ctggattata tcgtacagct ccaatatggt actgattgac
     4321 catattactc aagtaatttt tatcacgact agatcccata ttaacaaaca attgacctat
     4381 tttttcttcc aaagtcatag aatcaatcgt ctcttcaatc caagaaatag catcttcatc
     4441 taaattatat ggtttttttc gtaaatctac ttttatagtc attttctttc tcctctactt
     4501 attagtaaga tttttccaaa aagtatctat atatttttgt tcaatatcta cataatctcc
     4561 cgataaacat ttaaataaaa catcagttct taagttttcc catgattctt tttctttatt
     4621 gaccaaaata ggataaaaga aaactttatt tttatttgca gcttctagat caccaggaga
     4681 atcaccaatc attaatatct tatccaaatc acaaccattt tcaattacac ttcgaataat
     4741 atcttctttt tttcctttat cttgacaaca cagttcatca actaatgata ataattcatg
     4801 tctttcccat tcttcttgaa cagcttcctt attagctgag cttacaataa ttatcttccc
     4861 atattttttc agagctttta tagtatctct agcacttaaa aagggtttat ctaatccttt
     4921 tgcttgacta atctgttgat tgacttcttc cgaccatttt aaagctttta ataaatcctc
     4981 tgactgagaa tattctattt ccctcttcaa agatggatta gacaattctt ttgttagaga
     5041 tacccagtgc tttaatcgat taatcgatgt tacatcattt ttttctaatg ttttaactaa
     5101 acctaaaaat ctgtttattc cacgagtctc tgaatataaa ttaatattat tccaatcctg
     5161 taggaatttt tctctatgat gaacatcaaa aatctctgca gcaattggtc cgaaaaagag
     5221 attgtgttta taagtcatcg tatccataac acagccatct gaatcaatac aaaatatcca
     5281 attattcata tttctctcct atttatgttt ttgttttaaa agctcgcaat tttctaagac
     5341 tttatcttta gataatggat aaaggaaaaa taaaattaat cctacaatca gaaaacaaat
     5401 tgatggaatt aatgttgaag cgttatataa cttttctaaa acatctcccg tttgtacatt
     5461 tgctaaagag tcatagccta taatagaaag agtccaacct ccgacgcctc cagctagagc
     5521 ttgacctatt tttctagcaa aagaatatac cgcatatacg gtcccatctt ctctttgatc
     5581 agactttact tcttgatcat caataacatc agtaatattt gcccaaataa cagtattaaa
     5641 cacacccaaa cccatatagc caaccgaact aagaattaag aatacataca tattactagc
     5701 ttttatcaaa aataagatca aatatactat tgctgaaaag aacattccta ctgatgccag
     5761 ttcttttttt ccatattttt tacttaaaaa caaagttaaa ggcgaaacaa ctattaaaac
     5821 caaaatagga ttgataaaat tcattaaaac tattccctgt gcatcattat agtaatcagg
     5881 aaatacataa ttattcatac tcataattaa taattgagca aggagtaaga aaatagccgc
     5941 accaattata gaaagtaatg ccctattttt aactaaccct tttaaagatt ttaacaatga
     6001 aactttgtat tgagattggt caattcttac tcgttcagta gttagtttat aacatattaa
     6061 ataacaaata agtgcacata tcgaaaatat tcccgcaaca attgtaaaag ttgatccacc
     6121 acgaactatt tgagcgcctg aatcatctct tgtataaata aataatggtg ttaatacacc
     6181 aatgaccaaa cttgctaaag tagctccaac atttcggaat gtcgatagca ttgttctttc
     6241 ttctgtaata gaagtaatag ctgatgccat tgatccataa ggaatattta tagatgtgta
     6301 aaaaatactc ccccataata tatacgtaac atacatgtat actaccttaa cgctcataga
     6361 agctgaaatc atagaagttt gatacattaa aaaacttgct aaagcgactg gaccactcat
     6421 tcttaaaatc cacggtttaa acttaccctg cttagttgct ttcgatctat caacaatacg
     6481 ccccatagtt acatcagtaa ctgcatcgat aaatcttgct actaaaaata atgttccaac
     6541 ggcagctccg cttatcccca atacttttgt ataaaagatc attaaaaaag aactagcaaa
     6601 aatgaaagta aaatcgtttc caaaatctcc aaacatatat cctattttgt ctcttatacc
     6661 aaattttctt tccatacaat tctctccctt ctgttgttaa gcgttttcat tctgatgaaa
     6721 gtacgtacta tactctgaag cgtaaagagt cagtatttat tttttacatt tcagagtatt
     6781 atttgttttt tctcctaata attctttatc tcataaaact tatctatata tttactagct
     6841 actcgactta cttcttcaaa atcacctttt tcaacagaag ccaataaatt cccacctaca
     6901 ccaactgcaa caactcctac ttcaaaccaa tcttctaagt tctctatact tactccgcca
     6961 gtaggcatta tatttacgtg tggtaatgga gcttgatagg cttttacaat ggatggacca
     7021 aaagcagaac ctgggaatag tttaatgata tctacgccag cagtaagtgc ctcctttatc
     7081 tcagtaattg tcatacaacc tggtaaataa ggaatttgat ataaattaca aatctcagct
     7141 gtttgtcgat caaaagatgg actaacgata tattctgccc cagccataat tgcgatacga
     7201 gcagtaaacg catctaaaac cgtaccggct ccgattatta catctttttg atcagcatat
     7261 tttttagcaa tttctttgat tatttcttca gcattaggaa ctgtaaaagt tagttcaatc
     7321 cctttaattc cacccgcaat aattgaattt attgctttaa tggcctcatc cttatttttc
     7381 ccacgtacaa ccgcaatgac tccagccgct tctaacttac ttaaaatttc tacttttttc
     7441 atatctatct ccttccttta tggatacata ctttgtctag ctgtactaac ttacaaagaa
     7501 taaatttaag cttttatttc aagtattatt ttctacttcc gatttcaaaa aaattaaaat
     7561 aattatacgc attacgataa caaatattct ccactatctg actaagatac atttcatcat
     7621 ctggaattcg gcctgcttta acccactcgc ttagtaattg acataatact cgacgaaaat
     7681 actcatggcg aggatagctt agaaaacttc gagaatcagt caacatacct gtaaaattcg
     7741 acaacaaact ttgttgtgcc atcgtagtca actgttgacg cattccttca gctgtatcat
     7801 tgaaccacca agcacatccc aattggattt tttgaatcat acccccttgg aagctttgca
     7861 ttgctgttgc tagtggaagc caatcagttg gatttaatga atataaaatt gtttttggta
     7921 agcacccact cttttcagcg gtatcgaaca atgctacaac attttgtgtc aaactagctt
     7981 gatcccccat cgaatcacca ccgacatcta ctccaatttg ttcctttaat atacttgaat
     8041 tatttcttaa agcatttaaa tgcatttgca tcgtccaacc atatcttgta tactcagaca
     8101 tcaaatgtaa ttgaatacct gtaacaaatt gtgcaatttc tgataaatgg aaatccgttt
     8161 ttcctgctaa agcttttatt aacagattgt cgagtatttt ttcatctgct atttcataaa
     8221 aataagtatt caaaccgtga tctgctaatc gacagccaat ttgatgaaaa tgatccactc
     8281 gttggctcaa tgcttgcttc aacccattaa agtctttgat tggtatagcc gctgtttgac
     8341 taagttttaa aatatagttc gaaaagcctg atgatttgat actccagacc ttatctggac
     8401 ggaaagtagg taataccttg aaagaattat cctgtttaga aagctcagta tgataccaca
     8461 agtcatctgt aggatcatcc gttgtacaga tggctttcac atccatcatc tccaacaatt
     8521 ctttcgggcg ataggaatcc gtttgaattt tttcgttagc acgtcgccaa atttctggtg
     8581 cattcttctc agaaattgtc aaatcaatat caaagtaacg ttgtaattcc aaatgagacc
     8641 attcaaaaat aggattacct ggcgccttct ccactgtttt tacaaaagct aaaaattttt
     8701 cataatcatc acctggacca ctgatcaact cttcttctat accattagct cgcatcaatc
     8761 gccatttata atgatctcca gctcctttat catttaacca tacctgagtc aaattcttgt
     8821 attgcttatt ttctagcaaa tatgctgggt ctaaatgaca atgatagtcg ataattggtt
     8881 ggtgttttgc aaatttatga aaaagtcttt tagctatttc attctctaat aaaaagttct
     8941 tatctaaaaa catatttaaa cactcctttc cttccgttag tttttagaag agaaaatttt
     9001 tcctctaaat aaactccctt atataattat ttactcgccc ccttttcaag cgcttcccaa
     9061 ataccattta aataaactgc acctagagct cggtcatata atccataacc aggtctaccg
     9121 gtttctcccc aaatcatccg accatgatct ggacgaatat agccattaaa tccattatcg
     9181 tacattgttt ttaatatctt atacatatct agagaaccat attctgattt atgcggcgct
     9241 tcgtaaaaat cgagtccatt gtcacttaaa tatttgatat tacgaacatg tgcaaaagga
     9301 atccgatccc gttgacaaaa ttcagataga atttcataga catcattatc tggtcgttct
     9361 gcaatacttc cagtacataa tgtaatacca ttagaaggag aatccactac attacacaac
     9421 caatctaaat cgtcacgatt tttaacaata cgaggtaatc cgaaaatgga atatggtgga
     9481 tcatctgggt gaactgccat tttaatccca acttcttcac atgtcgggat aattgctttg
     9541 agaaaatatg caaagttctc tcgtaatttc tcttcatcaa cttctttata ggcttctaat
     9601 aattgcttaa tttgtgataa ccgatgaggt tcccaacccg gcaattcaaa attattagta
     9661 gaattttcta cttctctaat aatttcctcc ggatcagtag ggatattttt tgaaatatag
     9721 gctaacgtag aagacccatc atctaaacga taatccaaat ccgattttac ccaatcaaat
     9781 actggcataa aattataaca gataacctca atcccaaatt ctgccaaatt gcgaatcgtc
     9841 tgtttataat tctcaatgta ttgatctcgt gttggtaaac caattttgat atcatcatga
     9901 acattaacac tctcaataac tttcaaggta agtccattgg cttcgacttc atcttttaaa
     9961 gatttaattc gttctctcgg ccaaacctct ccggctggta tatccattaa tgttccaaca
    10021 attcctttca ttgctgggat ctgacgaatt tcttgtagtg agattttttc ttcatttgcc
    10081 ccataccatc taaaagtcat ttccatagtt actttcctcc aacttctgtt atatatttat
    10141 tgattgtttt acgaactgct tgaggaccta ctagcatttc gcaaaagatt ttttcagttt
    10201 tctttcctac cccaacttga tataaatcta aaccaaagat ttctttattt tctaaaattt
    10261 cctgtaccgc acaatgaatc tttgaaggat cttgttcacc taatttcaat actgataatt
    10321 tatcctgcaa ctctaccaat aacggatcag gacttggcac aaaattttcc ccttgatcat
    10381 cgactgccag taaataacgt aaccaagcag caatagcgat aggaataaat gttagatttt
    10441 ctgcatttcc gtctttttcg acatactttt taattgtttc accatagcga atcgctactt
    10501 tttgagaagt atctgttgca attcgttgtg gtgtatctgg aatataggga ttaggcaaac
    10561 gtttattaat tacttcatca ataaattgtt ttggatcaat aatgcctgga ttttcaacta
    10621 ctggtaaacc ttctaaataa cctattcctt tgattaaacc aaccaaatca tcatcctgca
    10681 tctctgctga aattgattga tatcctagca gacaacctgt tactgccaaa gcagtatgaa
    10741 gtggattcaa gcttgtcgta accttcatga tgtctgcttt atcaaccgtc tctctatctg
    10801 tcataatcac accagccttt tccaatttag gccggccatt tggaaaacta tcttcaataa
    10861 ctaaataatg aactacctca gtattagtga aagcagcaat attggtatgt ttttttgtgt
    10921 gaatgatatc taaatcctcg agccccatca tcaatagatc ttcctttaca ttctctgaag
    10981 gatttggcgt aattcgatca atcatactcc aaggaaaagt cactttttct ttatccttta
    11041 aataagcaat aaatttttca gaaacatatc cttctttttg ccaagcttct gcaatcgtaa
    11101 aaatactatc ttgaaaacgt tgaccattct gtgagaaatt atcagtagaa accatcgcaa
    11161 tcggcaactc tccagcatta aatctgctat ataacaagga tgttacaata ctcatcgtat
    11221 gttgaggatt gctaggtcca gatttgatat cagctttgac taccgaaaag aattgccctt
    11281 tagaatcttt taaactgtaa cctttttcag taatagtaaa cgttgtaaac tgtaatttag
    11341 gatcttcaaa atatttaacc actctttgat agtccgctat attcttcgga ttgcaatata
    11401 aactatcagc cgttactgcc aaaagtcttt ttttgagttc accatctcca ctcataacaa
    11461 tttccaaaat gtcattgtta tagtcatgat aagctttttc cactacttct tcatcaaatg
    11521 tttcacacac aactactcct gtttctaatt cattttgatc tgctaagcat tgagcaattt
    11581 ctgcatgaaa tcctcgataa agatttcctc caccaaaatg aagccatact ggatgttgct
    11641 ggccttcttg cttaacttct ttgacatcaa aacacggcag tagaatctgc atttcattga
    11701 attcttcgat agaattttgc caatttttaa tcgaaaccat aattaacctc ctcaaactga
    11761 tatgttagtt ttgatacatt ttatatttta aaacccattg taaccgttgt caatataaaa
    11821 agggaataaa cttgcttttt tctaccatat cagaatgatt gtatttttta ttcagaaaaa
    11881 taaaatactc cttctttttg gactgatttt atcgaaactt attttttctt tttttcatgt
    11941 attcttttat ttttataact agtgtattac ttcaaaattt gatattatat ggcatgaaca
    12001 gaattaaaat atcagttaaa ataaaagtta aggagcattg tgtatgacaa ttaaaactag
    12061 tctccaaaat caagcttacc aatctattcg ccgacaaatt atctatgctg acttagaacc
    12121 tggaaaaaaa gtatcagaaa aaaaattaga agaaacactt tctattggtc gtacaccaat
    12181 acgtgaagca ctgattcaac tccgacaaca aggtcttgtt gatacaattc ctcaaagcgg
    12241 aaattatatc tcattaattg atttagattc ggccaaaaat gctcgatttg tcagagaaca
    12301 tttggaacga caaattatgt tagagtgttg tgccaaaatg aataaccaaa aaaaacggat
    12361 tttagaaact ataatttcag aacaagaaaa agctgttagt caaaaaaatg ctcgtagttt
    12421 ttttcataat gataatttat tccataaagc ttgttttgaa attgcagagc gagaagaagt
    12481 atggcaatgg ttagaaaata acaatacaca cttagaacga tttcgatggc tacgagtaaa
    12541 aacggaagga ttaaagtggg aaaaaattat ggaacaacat tatcaactat ttgaggcatt
    12601 aacaaaacaa gatccagaag aagctaactt tctaacatcg ttgcatcttc atttaatgct
    12661 taatgagcaa aatattgttt tagctactca tccaaaatat ttcaaaaata tataactttt
    12721 aactcttttt aaattaaatg gttgttctga tcagaaaaat atcgcttgtt ttcttattat
    12781 gtaatatatt tcataaacta ttctgcataa caactattgt ttgtactttt tacattggat
    12841 aactaattat gactgctgat aacttttgat attaaactag tatctccaat acaaataaaa
    12901 aatagatatc ttgttcatca aaattagttc gtggatactt accttcattt acatcattca
    12961 gcggtttccc attttctttt attctattat ttcattttct aatagacttg gcttcatttc
    13021 gcaatacttt ttttcaatag gcatcaaatt ggtgttgttt agatatttag ttgtagtttt
    13081 gtgacatgat aagtatgctc ctttttctct tttccactaa tacaacgatc aaagggtgct
    13141 tttttcaaaa aactagaaaa atcaaaataa gattcgtatt ttgattctat atatcctgtt
    13201 tcaactacat cttatatttc acaatagaat aaatagcctt ttgcatagct gcttaactta
    13261 taagaaattg caatttactt cttcaaagtg aaaacgaata tgagtaaaat aaaattattc
    13321 ttcttacaga taattaaaaa tagcgcgtaa caaaaatcgg cattcaatca cataattagg
    13381 attgatgccg attacttata ttctacgtat agttttagta ttcttatttt ttatcaccag
    13441 taaccaattg agaacagccc taaagcagaa tagtctttta tctcaaaacg cttaatttca
    13501 ggcttaggaa tatgaataat cttttgagta tattctttct cttttaatct tactgaagaa
    13561 ataccactgc ctatcttttt tacaaatatt tcatttggtg tacagactaa taaaacaata
    13621 tttgtaatca atccttgcac taaagaatta aaaggaagac tatttaaaac agggtgtgca
    13681 taactataac atgtgaagta gttattttga actttaactt ttaaaaactt taaaaataat
    13741 cttatttgtt cttctaaaca atcatctata ctcatataat cactccacta attaatctcc
    13801 ccaaaaagac acagcattaa gagatatacc aacacaacag tgcaaataac tctacatctt
    13861 ccttcgcaac cttatttcaa taaaccataa aaattatttt ttaatagaaa ctgaaaatta
    13921 aaactagaaa aagtagtttt accgaatgta agagcatc
//
LOCUS       DAYYCW010000074        13760 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000074 DAYYCW010000000
VERSION     DAYYCW010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.076"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            232..570
                     /locus_tag="ACHAYA_002022"
     CDS             232..570
                     /locus_tag="ACHAYA_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605811.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(775..1337)
                     /locus_tag="ACHAYA_002023"
                     /pseudo
     CDS             complement(775..1337)
                     /locus_tag="ACHAYA_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(2069..2650)
                     /locus_tag="ACHAYA_002024"
     CDS             complement(2069..2650)
                     /locus_tag="ACHAYA_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8605812.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2833..3459)
                     /locus_tag="ACHAYA_002025"
     CDS             complement(2833..3459)
                     /locus_tag="ACHAYA_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8605813.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3481..4812)
                     /locus_tag="ACHAYA_002026"
     CDS             complement(3481..4812)
                     /locus_tag="ACHAYA_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM8605814.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4967..5368)
                     /locus_tag="ACHAYA_002027"
     CDS             complement(4967..5368)
                     /locus_tag="ACHAYA_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8605815.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5454..5660)
                     /locus_tag="ACHAYA_002028"
     CDS             complement(5454..5660)
                     /locus_tag="ACHAYA_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605816.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5755..7257)
                     /locus_tag="ACHAYA_002029"
     CDS             complement(5755..7257)
                     /locus_tag="ACHAYA_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM8605817.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7275..7730)
                     /gene="argR"
                     /locus_tag="ACHAYA_002030"
     CDS             complement(7275..7730)
                     /gene="argR"
                     /locus_tag="ACHAYA_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8605818.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7828..8445)
                     /locus_tag="ACHAYA_002031"
     CDS             complement(7828..8445)
                     /locus_tag="ACHAYA_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8605819.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8666..10039
                     /locus_tag="ACHAYA_002032"
     CDS             8666..10039
                     /locus_tag="ACHAYA_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8605820.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10114..11226
                     /locus_tag="ACHAYA_002033"
     CDS             10114..11226
                     /locus_tag="ACHAYA_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8605821.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11298..12935)
                     /locus_tag="ACHAYA_002034"
     CDS             complement(11298..12935)
                     /locus_tag="ACHAYA_002034"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM8605822.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13103..13711)
                     /locus_tag="ACHAYA_002035"
     CDS             complement(13103..13711)
                     /locus_tag="ACHAYA_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8605823.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3713 a         2892 c         2459 g         4696 t
ORIGIN      
        1 ccctttgtaa actatctaga aaattgtttt gtttaaagta tatcttattg gatataaata
       61 gaaataaatt gtagccagtt cattttttct ttaactaaaa aaataatcca agaaacattg
      121 aacctatgga tccaaaagct acccaaatac tatagctaac cccccgctac cttcaatttt
      181 gcagataaaa cgctttggga gaaaatttca aagacaacag gccatcctat catgcccgat
      241 tataaacaaa aagctggcta ttccttttgc tctgacaaat ttcaattggg aaagttcacg
      301 aaactagcag gtgaagaagt ttcaactgtt cggatcaaag aatgcccgct tcaaatagaa
      361 acattggttc aacgaatttc aactagacaa gactttgcta ttgtagaacg tgagatcgta
      421 gctatttttg tagaagaagg gattttacat gatccaactc atatcaatgt ggataaatgg
      481 caacctctta tctataaatt cagagaatat acaactgcca accagtcgtt aactttgaat
      541 tttagattcc aagaattcaa agagtcataa tgataaacat cgttaggtat aaataaaaaa
      601 gactcgcagc acgagtaaaa aacaaataga agctatacat tagaaaggtc gactgtgacc
      661 ctatagatgc agtatatgca gcttatgagc atttcttctg ttctctttgt ttttgggatc
      721 tgtgctgaag tctttttcgt tttttgccgc tctttctttg aaaaatcgat tatgttatac
      781 cttctccatt actaattcag gcatctgctg agtaatgatt tttcgcgcaa aagaaatatc
      841 tgttacccaa ggttcgattt gttcttcagt caagacaagc ggcattcggt catggattgg
      901 tgcgactgtg cggttaggtt cagtagtcat tatgattgat tctgccattt tttcatcctg
      961 atggctacga taaaaaccgc caatatacac tattttttcc ttatttgaaa acataaattt
     1021 gtctttttct tcattccact cattgggaaa acacaacgat tttgcaaaaa tggttgatga
     1081 aaggtctttt tttcttctac tgtctctgca cgggcattaa tcatcaattg tcctttttta
     1141 aatccggtaa atccccactt catgatttcc ggagtgagga tgttttcttc ctcttttctt
     1201 aacgtcacaa caagatttga cggaaaaact tctccagctg ctaattctgc tgcctgcttc
     1261 ggttgtttcg tctgggcttc tcgataataa ttctgaagga gctctgactc tagatcaaaa
     1321 aaataacgac cacacatcct aatccctccc aatctttttt tctatcatac actgcaacaa
     1381 gaagttcgca ccaacaaaac gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg
     1441 acaaactctc cagtgaatgg tattctcctg ccaactcctc ggtcacaagt cacaatattc
     1501 aacaaacatg agaagctgct ttttgaaatt gttccttgtg actcggagtg aaacggcagg
     1561 cttacgacct ctgggtaaac ggtgttcaaa agccagtttc tcggtattaa gccaaattta
     1621 tgcaaaaatg tagaaagcta ttttcacaaa atttttctta atactcgaaa ctaagcgact
     1681 ttttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccaa
     1741 ccaaacgaaa aatatgagca gctattttta tttggctgtt cttattactt gatgcagggc
     1801 agctttttca caacctctcc actaaacggt gttcaatcag ctaaccttcg gtattagctc
     1861 aaaaaacggc aaaatatgag gaactatttt gccgtttgtt tctctaatac tcaggtcaaa
     1921 gcgctgattt cacaacctct aatacacggt gttccagaag tagcttcttc gaaaaataag
     1981 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
     2041 tgaccacttc tgtcacaacc tctaattatt aaagaacctt ttgcgaagtt aacaatcttt
     2101 ctttgatcaa ttgaaaattc tgcctaagta gtgatagatc ctgtgtgact ttcaacaata
     2161 agatacttcc ctggatttca gcaatcaacg ctttggcttg acggtctgct tcttcatcgg
     2221 acagtttttc ttcagaaagt gccaggatat tttgtttcgt atacttgacc cattgattga
     2281 ctaattctag tagcaacaga cggtattctt catccactgt actcaactca ataatcaaat
     2341 tacctacagg acagccatga taagtgatct gtgtctcatg atcagcgaaa atccagtcca
     2401 acatttcttc taatgcttct tctggctgtt ttccagcatt taagatgtct tcgaacaatt
     2461 ctgtctgcca tacgtccata tgcgcatgga tcaccgctag acaaacttct ttttttgaag
     2521 aaaagtaata ataaaattga ccttttcctg tatcagaagc tgctagaata tctcggattg
     2581 acgtttcatg atagcctttt ttataaataa gatctttggt cgcttccaag atcttttcct
     2641 ttgttttcaa tgatattctc ctttttcatt tctcatagtc ctatattata cagtaaattc
     2701 atcatttttt cttcttaatc gtttaaaaaa gactgtgcca aattatctga cacagccctc
     2761 actataaaac tgttctcata tagctagatg ttcattgatt caagaactgc gaggacaaaa
     2821 gcaattccta tcttatgata gttgctctaa tacttcctct gttgtcatca cttgatctgc
     2881 aataaagcca aagttcgtca acgctgcatc cattgcttct tggccaggag caccaattgc
     2941 atctgtcgct accacaacat tgaatccact ctctaccaat tcacgcatat gggaatcgct
     3001 acacaagttt ccgttcattc caccaagaat tactgtgtcg attccatttt ttctcaattg
     3061 gagcgccaaa tcattgcttt ctggtccaaa gattttatga ggagagctga tgatcgtatt
     3121 ttcatccaaa taaggtttca attcttcgat aaaatcggct cctgaacctt cttccactga
     3181 ttcatattga ctttttctag caaacatctg ctgctcaagc atcatatgtt ctcctgcacc
     3241 gccaaattgc catttatggt catgtgggta aaaataatga ggagaaataa agagtttgta
     3301 tcctttttct tttgcttgtt ttaccatttc aatcatattt tcgatcgtat tgtatttttc
     3361 taagatatct tttgtcaaac caaagccacc gcctgctggt ttcaagaact cgacttgcgg
     3421 gtcagtgaga actactgctg tatttttatt attgaacatg gtggattctc cattcgttat
     3481 ttattttaat acatcattta gagcttcact cattgtcgga tgagaataga tttgatctct
     3541 caataacgta taaggaagtt ttccattcat agctagcgaa atcaagttga tgacttcatg
     3601 agaatcttcc gcatagatac ttgcccctag aatcaagtca gtttcagggt cgactaaaac
     3661 tttgaataca cctttaggat cttctaatac ttgtgcttta gggacaccgc ttgtcatata
     3721 cttgaataat ttgtaattga cgccttgtgc tttggcttgt ttttctgtta aacctacatt
     3781 ggataaagtc ggagtgataa atacactgta aggcacagct ttccggttat tcgttgttcg
     3841 tttattctcg cccttcaact gatccataat gattcgataa tcatctagag agatataagt
     3901 gaactgcaat ccgcctttga cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag
     3961 gtattcatcc acagcgattg ctccgcgatc tgttagttta acatctgtat tttctaatcc
     4021 aagtgatgct gtacttggtt ttcttccagt tgcagccaaa acttttgacg cttggattgt
     4081 tacttcttgg ccatcttctg taaaggtgac ggcagctgtt gattcttggt ctgctacttg
     4141 atctacagat acacctagat ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat
     4201 ttgcgcgata tcttcatctt cacgtggaag aaattcagaa tgggcatcaa gaacgacaac
     4261 tttagatccg tattcattga acattgatgc aaattccaag ccaatatatc cagccccgat
     4321 gatcactaat ttttccggta attgttcctg atccatcgct tgcgtagaat caatcaagta
     4381 gcgacttgta ttcaatcctt tgattggtaa aatgattggt tcagaaccag tattgatgaa
     4441 aatccgttcg ccttcaacaa tcactttttc accattattc gtcacttcga tttgatggtt
     4501 agaaagaaaa cgtgctgtac cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg
     4561 ataatttttg gcgcgaagca tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa
     4621 gtcgattcct ctttctccat tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat
     4681 acatgttccg ccatacatac gtgttgactt ttcaacgact aaaaccgatt caccttttga
     4741 agctaacgcc ttcgctaaag tcttaccgcc ctttccaaaa ccgatcacaa tattttgata
     4801 tttttccatc atttttccct cgcttctttt ttacctaccg attggtacaa aaatatgata
     4861 accgataatt gtctattctc caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa
     4921 agcggtcctt tcacgttttt agtctggttc gatttccttt attcttctaa acaatcgtta
     4981 cttctacatc gatatttcct ctagttgctt tggagtaagg gcagaattca tgcgcatctt
     5041 ctgcaatttt ttgggcttct tcttcgctga tgccatcgat atgcacttct aatactacac
     5101 cgatctgata gccgccatca tctaaattat aaagagagac acgtgctttt actgtggact
     5161 ctgctgtcat tcctgcttct ttgatcatat gttgtaccgc cccattgaaa caagaagcat
     5221 aacctgctgc aaataattgt tctggatttg ttccttctgc atggattcct ggaggagtga
     5281 ttttcatatg catcgagcca tttggtgcct cgacttctcc attgcgcccg ccatggttga
     5341 tcattgctgt ttcgtatgct tttttcattg tactgctcct ttcgattgac catttgctac
     5401 ttggctataa ttatagtgta tgacaagtaa ggcgctttct acaaaaattc tgcttattcg
     5461 aataccagaa tgccgtcttc tactattttt ccaaaaaaca aaaaaatact actcgtgaac
     5521 aacagcgtca tatttgttcc aaaataaata gcaatctttt ctacataatc ttttatatga
     5581 ttgattttaa gatacatata taccagaaag ttgaccccat aaaccaatac tgttaaaaaa
     5641 ataaccatat tcgttaccat tttttcctcc ttttgctggt ctccctacca gcactcgatt
     5701 taaccatacc actttttttg ccgtccaagc aactagcctt tcaaaaagac tggtttattc
     5761 tgctgttaat gattcaagat cagaaaaatg tccgaataat tttgctaatt ttactaacgc
     5821 caacttgttc tttccgaatt catcattttc tatctgtaat actagtaacg gctcatgtaa
     5881 gcttaaacgg atcaaaaacc agccgtttcc gtatggctct tctagatcat atcgaatacc
     5941 ttcttcattt tccgggtcga agttcatccc tttttcttct tccaacagtc gcttcatttc
     6001 ttctaatatc ttgtttcctt gttcgcgtgg ttcatcgctg aacaatttta atcggatttc
     6061 ttgtacttct acgggctgtt tcaactcttt gatttgttca tctaatgtga taccttttct
     6121 ttttaattct ggcaaaagca tcaggatctt tgcgatgaca tatgcaccat catccagaaa
     6181 atagttttcc ttgaatgcag cgtgtccgct tgtttcgatg gctagctgtg tatctttccc
     6241 aagacggtta agttctaatg ctttattgat tacatttcga tagccggaaa tataacgaac
     6301 ttggatgcct cctttttctt caatgaatct ttgtagatga gaagaagtag gggaattcgt
     6361 cacgattctt gttcctggat gctcttttaa tacgattgta cttaatactg cgatcaaatt
     6421 atttcgattg atcactgttc cagaagcact gacaactgca gaacgatcca catctgtatc
     6481 gaaaatgatc ccaagatctg cttcatttgc taaaacagcc tgcttgatac tttccatcgc
     6541 ttctttatta tcaggatttg ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg
     6601 ggaacctgtt gaatccgcac ctaagctatt caatactttt tcagcaaaga atccaccagc
     6661 accatttccg gcatcaacaa tgattttcca accatttagt ggtttgcttt ctagtgttcc
     6721 aatccccttt ttaatttttg tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt
     6781 ttctacgctt ccagtttgtt cgctttttat tttttgctgc tgtgatagga taaaagcaat
     6841 atcttctttt tctgctcctc cttctgaggt aaagaatttt attccattat aataataggg
     6901 aagatggcta gcagtcagca taaccgcagc atcgcacgag aactgttcaa attgagtgga
     6961 cataaacata gcaggcgtag tagctaattc gaaatcgatg acatgtatcc cttgttcttt
     7021 cagactttgg atcaacgcct ctttcagtgc tggtccgctt atcctactgt catgaccaat
     7081 cccaatcgtc cattttttat ttggatcggt tgatttcttc tttttcttta tccagtttgc
     7141 tagtccccag ccgatctttt ttaccatgtc tgtcgtcaag tttacttcat atccttcaac
     7201 tggtaatgcg attcctcgaa tatccgatcc attttgcagc gctttcatat ctttcatata
     7261 aaagtcctcc tcatttattc cctcattttt tccagctttt ctgcaaatcg ctgtgcttct
     7321 tcttctgagc ggcaaataat aatgaatgta tctacaccgg ccatcgttcc gacaacttca
     7381 gggtaagcca cttcatctag ccaattcgtt accacatcgg caccatttcg ttcagtcaat
     7441 acgataacca tgaactgaac agactggata cgcaaaacct cgcgtctaat tgctgtacgc
     7501 aaacgttctt catttaaaga aacagatggc tggttaaata atgtatagcg tactattttg
     7561 ttttcatttt gactttttac taacttcatt tctcgaatgt ctcgagaaat cgttgcttga
     7621 gtcacatcgg cgcctgcttc atgcagccgc gcaagcagct cttcctgtgt cccaataggt
     7681 tgttctgtaa tgatctggcg gatcagtgct tgtcgctgtt tctttttcat attagacacc
     7741 tctcaatgtt gattatagca aaagataagt aatatttctt catattttgg tattttttac
     7801 ttttattttt tgttttcatt caggttgcta acctttttct attttaacac gcaaaatcgt
     7861 cttcattttt tcaggtgctg attttcttgg atcgctgatg taaatctctt tgtgttcttt
     7921 agaagctctt tcatagcctt cttttgcgat gaatgcttct aatttctcaa acgttgctgg
     7981 ttcttcggca tagggcccga cgtgtaagat ttgggcacaa agaccttctt ccatcttttt
     8041 gaatactact tgttctttca agtcttccgg aagtttgcta gaacgctgaa tggcttcttg
     8101 tgctatcgct tctgtaacaa agtcaggctg tcggatcatc aattgataag aaaaatgttc
     8161 ttttttcatc actggttcat gcaaatactt ttcttgcagt ccccattgtc cttccagtgg
     8221 atagacagta tacatctgat aatcaggaat cagccaattt tttttaggcg acatgcgtat
     8281 tgcatagctg actgcatata gacaggctac tcttcgttta aagtcttcct gattcgggtc
     8341 gccgatccct ctgatgctaa agaatgattt tttaggaacc tctagtattt gcgggctttt
     8401 tttcatacta tagctcttgt ctttacgcca ttcaattttt tccatttttc ttcctccgcg
     8461 aaattccatt tagttattta ttatcatacc tctttttgag tgacttggct aatcgtgttg
     8521 acactatttt tcagtttgtg ctagaataag tgaagtaaat aaatcgtaca gataacacgt
     8581 ttcggcgtgt cgagagagag gcggtttcta tttttataat agaaatcact ctcttttttt
     8641 acgaaatttt ttagaggtga tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc
     8701 agtaattgca acagggatga tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt
     8761 aactttccca actcttatca gacagtttga tttaacgact gcacaagtcc aatgggtaac
     8821 aacgatttat cttttgatga tttcgatcat tgttccgctg tctacttatt tgaatcgcaa
     8881 ttttagtctc aaaaaactct ttttagcttc aaatctgctt ttcttggcag gtataatgat
     8941 cgatttcttc tctcctacct tcatggtcct attgtttggt cgattgctcc aaggtgcagc
     9001 gacaggtatc gctcttccgt tgatgttcca tattatcttg acatttgccc ccctcaatcg
     9061 acgaggcgcg atgatgggtg tcggtacttt gacaacggcc attgctccag caattggccc
     9121 cacttatgga ggcattatga cttcacattt tacatggaat catatcttcc tgtttcttgt
     9181 gcccgttttg tttctttctt tgattatcgg ctggtttgct attcccgaga tggcagtaca
     9241 aaaaagtggt tatttggatt ggataagcgt tttaggaatt tgtcttttgt ttagtggaag
     9301 tctaacattt ttgagtacgt tgggaacagt ttccagctgg attgctttaa tcatcggcgg
     9361 tattggctgg ttcctttttt actatcgaag taaaaaagtg gagcatccac tgatccgact
     9421 gtctattctt tcaaataaaa cattccgctt gtttttgatt ggcttcttag tttatcagtt
     9481 tttattactt ggtgtatctt ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag
     9541 tccttttgtc gctggattag cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc
     9601 tttttccggg aaaatgctgg atcaatatgg tccgaagaag ccaatttttg ctgggctgat
     9661 tctttctttg attggttggc tagcattatt cctactcata ggacacgctt cagtgggtgt
     9721 attgattgct tgtcatgtgt tttatatgat cggtatcgga ttatcttaca gcaatatgat
     9781 gaccaccgga atgaatgcac tgaatgaaga actacaagga gacgggaacg caatgttcaa
     9841 taccttgcag cagttttctg gcgctgtggc tacttcatta gtagcggtca ttatcaatta
     9901 cgtccagcat catacttccc ataactatga agtgtctact acattaggat caaaagcagc
     9961 attaggcgtc ttgttgctgt tgcttctcgt aagctttgct cgattcgcct attatctgtt
    10021 ctttgctaaa aaagcgtaga ttccatatac ggcaggtgtt gcatctgccg tttttttgtg
    10081 tgaaaatggt tgtttgtaaa aggaggcttt tttatggaaa aaattgccat tatcggtggc
    10141 gggatcattg gtatgaccct agccagttac ttagatacat caaaattcaa tgttgcttta
    10201 tttgatgaag ggacaggaca ggcaactaaa gctagcgctg ggattatctc cccttggttg
    10261 tcgaaacgca gaaacaaaca atggtatcgt ctggctaaag acggcgctgc ttttttccct
    10321 aaacttataa aagatttcca acttgatcaa acgatttacg cacaatcagg gacacttttg
    10381 cttcgacctg aaaaagattt agctgaattg gctgttcttg ccgagaaacg aaaagaagaa
    10441 gcgccagaaa ttggaaacat cgaatggctt tcacctgaac agacatctca agcacttccg
    10501 ttattgaaac cagcagtttc actgcgtatc agtggtggtg gtcgtctgga tggaaaagca
    10561 tatttagaac atcttggtca gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa
    10621 gtcactttgc ataagaagag cgattcttgg ctcatcaagg gaccgtttgg agaatacaca
    10681 gctgatcgtg taattttgac acctggcccg gcacttaagc ctttattaca agaattaggc
    10741 tatgctgcag atatacgtcc gcaaaaaggg cagctcttag catttcagac atcttataag
    10801 atgagtcaag actggcctgt tgctatgctt gacggagaag ctgatttcat tcctttcatg
    10861 gatggaaaga tccttttagg tgctacccat gaaaacgatc aaggctggga tttggctcct
    10921 actagcgaag cttataaggg attgactaag agtacagaac agttcttgac tgatccagat
    10981 cagataaaaa actggccata cgagttccgt gtaggtaccc gtgcttatac gtcagatttt
    11041 tccccatttt tcggcgtact gccagatgat ccaagcttac tggctgctag cggtctaggc
    11101 tcttctggct tgaccacagg tccttatatc ggctatctat tagccaactt cttaaatact
    11161 ggtcattggc ctacggaaga ttatcaaaag gacatctcca cctatattac gaaaaatcca
    11221 tcatagaaaa acagcttttt caagtaagga tcccgagcga tattgcgcat ggattacttc
    11281 ttgaaaaagc tgttatttta gttcaatttc tgaatcaaat tctccaaact ttcttttgct
    11341 tcatctggca agagatcttt tcctatctga gcttcaatga atcgtaaacg aatatcaagt
    11401 cgttgtttca ccagattttt ataggacgta tcctcaaaag aaacaggaaa atctggtata
    11461 ggtaaatagt ggatgtcttc aaatggacca aacgacttga attctgccgt ttgttgcacg
    11521 attctttcaa cgattgacaa tgtcgtttca ggtttgattt ttttatcatc gaaatatcca
    11581 gtattcagga catagcaggc tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag
    11641 tcttccctaa gaggatagac acggaaagga ttagcaaatg gttcaatgac tagctcatct
    11701 agctgtcttt ttgtagtgat attttccgcg tctgatcgtt ttgttgctaa tgttgcacca
    11761 aaagacgctg ctaaaactgg gtcattgatc ttgataattg gcggaagcga atcatctttc
    11821 atgatccaaa aaacagcatc gatcttttcg ctaagatgat cgactcggtc gggtgtcgca
    11881 taacgtgatt tgactgtccg cccatttccg tttcgtatat ctcctgttac taggactttt
    11941 tctcctttac ttgtacatgt cacgcccaca ttctgacaag taaggaaaga agaaatggcc
    12001 ggatcatcag acggatagtc ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca
    12061 aaaccatttt ttcgagaaat aacgaaagca tcgtcatgca agatcgaaac agagtgacct
    12121 tccgtatgtt ttgctaaggt aatcgttgat tttccagaac cggacaaccc aaatacagcc
    12181 atcgtatagg tctggtcatt actagcatat tgtttcatcc caccgtggca ggcaataaag
    12241 ccattgcgat ttcccaatgc ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag
    12301 taacgtaatc ctagcacagc cgctgtatta tggagagcat caaacaaaat gagtccattt
    12361 ggaaatgttt catgtgtcca ttcgggatca caaaagatat aaagatctcc ctcatcaaag
    12421 gaaacagact tttgataagt ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa
    12481 taagaataca aattgttctc aaatccttct ggaatcagca aatgactttg cacagaaaat
    12541 tcttcagata ggccaacgat tacttttgta tggtaaaact ggcgcttgcg actttgatac
    12601 acagcttctc ttaaaatttt ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc
    12661 cgccgagcac ttgctgttct tcccacaatg cttccatcat tgaatactag tgtcctaaca
    12721 tcagctggta aatctagttc ttcagcatgt gagattgtta aatctgtgat gatcgttcct
    12781 ggactccctt ttgctagttg ataagctgtc ttcctatcaa aaacttcatg aacattatta
    12841 ccgtaaaatg tcgtttctac aatcgatcga atactggata acagtggatt tccacttgtg
    12901 atttcgttta attcgagact ctcttttgta ctcatggctc ctcctcattc cctaaacaaa
    12961 aaaacgtttt ctttattata cattattcga gtttttattt gtactgctgc gctgtgggaa
    13021 gcaaacgagt tatttttcac acaaaaaaac cgagaacttg aataaacaag tttctcggtt
    13081 tcatcatttt ccttttatcg gcttagtacc agccgtttgc taaccagaat gttttagcag
    13141 catcccatga accataacga gatgttacat attgttctgc tacacgttct tgattggcag
    13201 ctgaatagtc gccgtttaag tatgaagcat ctaattgata acggccgatg taacgtccgt
    13261 tagtagctgt gtatgaaccg cctgattctt tttgtgcgat ccattctttt gcagagcttg
    13321 aagttggcgc tgttgcagca gctggggcag caggagctac ttcaactgtt tcaacaggtg
    13381 caacgtatgt atgttcttgc gttgctgttt cagttgtaga tgctgtattt tctgttgttt
    13441 tgttttctgt tgttgctgaa gaatcatttg aagttgggat tgttaattgt tcaccaacat
    13501 aaatgcggtt gatgtttgcg atcgaatttt tttcagcaat tgcatcgatc attgagttgt
    13561 ctcctgcaaa tttttgtgag atcttagata atgaatcacc tgattttact gtataaactt
    13621 catcagcgtg agcatttgta gttcctacaa acattgcagt agcaccagca gcaaaagttg
    13681 tacctaaaac aattttctta aatgaattca tattaaactc ctttttttct ccgtttttta
    13741 ttctttcgac gctattaact
//
LOCUS       DAYYCW010000075        13665 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000075 DAYYCW010000000
VERSION     DAYYCW010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.077"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..307
                     /gene="yaaA"
                     /locus_tag="ACHAYA_002036"
     CDS             65..307
                     /gene="yaaA"
                     /locus_tag="ACHAYA_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM8605824.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            294..1418
                     /gene="recF"
                     /locus_tag="ACHAYA_002037"
     CDS             294..1418
                     /gene="recF"
                     /locus_tag="ACHAYA_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM8605825.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1415..3361
                     /gene="gyrB"
                     /locus_tag="ACHAYA_002038"
     CDS             1415..3361
                     /gene="gyrB"
                     /locus_tag="ACHAYA_002038"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM8605826.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3381..5852
                     /gene="gyrA"
                     /locus_tag="ACHAYA_002039"
     CDS             3381..5852
                     /gene="gyrA"
                     /locus_tag="ACHAYA_002039"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM8605827.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6088..6387
                     /gene="rpsF"
                     /locus_tag="ACHAYA_002040"
     CDS             6088..6387
                     /gene="rpsF"
                     /locus_tag="ACHAYA_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM8605828.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6437..6949
                     /gene="ssb"
                     /locus_tag="ACHAYA_002041"
     CDS             6437..6949
                     /gene="ssb"
                     /locus_tag="ACHAYA_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8605829.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6975..7211
                     /gene="rpsR"
                     /locus_tag="ACHAYA_002042"
     CDS             6975..7211
                     /gene="rpsR"
                     /locus_tag="ACHAYA_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM8605830.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7354..9327
                     /locus_tag="ACHAYA_002043"
     CDS             7354..9327
                     /locus_tag="ACHAYA_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM8605831.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9333..9785
                     /gene="rplI"
                     /locus_tag="ACHAYA_002044"
     CDS             9333..9785
                     /gene="rplI"
                     /locus_tag="ACHAYA_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM8605832.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10059..11426
                     /gene="dnaB"
                     /locus_tag="ACHAYA_002045"
     CDS             10059..11426
                     /gene="dnaB"
                     /locus_tag="ACHAYA_002045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM8605833.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12206..13498
                     /locus_tag="ACHAYA_002046"
     CDS             12206..13498
                     /locus_tag="ACHAYA_002046"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM8605834.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4557 a         2469 c         3010 g         3629 t
ORIGIN      
        1 gttttattgc gaaaaaaaag gtataataga gtatactttt aataatgaaa atgagggatg
       61 ggcattgaaa caacaagtca ttctaaatac ggaattcatg acgttgggcc aaatgttgaa
      121 agaagtcaca gttatcggta gtggcggaca agcaaaatgg tatttggcag aaaacactgt
      181 gttagtcgat ggcgaaccag aaaatcgtcg tggacgcaaa ctttatcccg gcatgatggt
      241 tgaagttcca gaagtaggaa ctttttttat ggtaaaaaaa ggaaacgacg ataatgaggc
      301 tgaataagct gtatttaaaa aattatcgaa attacgaaga gctgaacatc gaattttcca
      361 agaacctcgt catcttttta ggagaaaatg cgcaaggaaa aacgaatctt ttggaaagta
      421 tctacgtatt agctatgacg cgaagtcatc ggacaagtag tgagcaggaa ttgattggct
      481 ggactgacga tcaggcgatg attcaaggag agatcacaaa aggttcatcc actcttccat
      541 tagaaatctt attatctaaa aaaggccgaa aaaccaagat caaccatatc gaacagaaaa
      601 agttaagtac ttacgtggga caaatgaatg tcattttatt tgcaccagaa gatctttctt
      661 tagtaaaagg cagcccgcag atcaggcgga agtttttaga tatggaatta ggccagatca
      721 atccaatcta tctttatgat cttgtccaat accaagcaat tttgaaacaa agaaaccagt
      781 acttgaagca attgaatgaa aaaaaacaaa ctgatttgct ttatttggat attttgtccg
      841 aacaactagc agcgtttggc agcaaagtat tgaaagcaag agcacagttc gtcaagcgat
      901 tggagttttg ggctaattct ttgcatcagc aaatcaccca tcaaaaagag cagctggaaa
      961 tcgagtatct caccgcagtc gattctttag aaacacatac gcaagagcaa atccaagaac
     1021 aatttctcgc acttttgaac caaaataaaa agaaagacct ttttcgggga acaacgacag
     1081 ttggtcctca tcgtgacgat ctttcctttt ttattaatca gaaaaatgtg cagacttatg
     1141 gatcgcaagg acagcaaaga acaactgcac taagtgtcaa attagcagaa atcgatttga
     1201 ttaaagaaga gacaggggaa tatccaatcc ttttgttaga tgatgtgatg agcgagctgg
     1261 atgataatcg tcaactccat ctgctagaaa caatcgaagg aaaagttcaa acgttcttaa
     1321 caacaacgac acttgaccat gtaaaaaata aaatgactgt tgaacctgag attttttatg
     1381 ttcaacaagg gaaactaaaa ggagaagaaa ctgaatgaca gaagaaagaa gtttagtaga
     1441 acgcgctaaa gagtatgatg ccagtcagat tcaagtactt gaaggacttg aagccgttcg
     1501 taaacgacct ggcatgtata tcggttcgac tagttctgag ggtctgcatc accttgtttg
     1561 ggaaattgtc gataattcga tcgatgaagt attagcaggt tttgcaacga aaatccatgt
     1621 gattattgaa aaagacaaca gtatcaccgt tattgacgac ggtcggggga tacctgtaga
     1681 tatccaggca aaaactggac gtcctgcagt tgaaacggtc tttactgttt tacatgctgg
     1741 aggaaaattc ggcggtggtg ggtacaaagt ttctggtgga cttcacggag taggatcttc
     1801 tgttgtaaac gcgctttcta cacagcttga tgtcaaagta tacaaagaag gaaatgtgta
     1861 ctatcaagaa tatcgtcgtg gagctgtagt ggacgactta aagattatcg aacaaacgga
     1921 tcgccatgga acaacggttc actttacgcc agaccctgaa atttttacag agacaactga
     1981 gtttgatttt tcaaaattgg ctacccggat ccgtgaactt gctttcttga accgcgggat
     2041 gcgtatatcg attgaagaca aacgagaaga agaaccagtc atcaatgaat accattatga
     2101 cggcgggatc aaaagttatg tcgaatactt gaatgcaaac aaaaccgtga tttttccaga
     2161 acctgtatat ttggaaggtg aacagcaaga tatcgcagtt gaagtctcta tgcaatatac
     2221 agacggctac cattccaata tcatgagttt tgccaacaat atccatacgt atgaaggtgg
     2281 aacacatgaa tcaggattca agactgcttt aactcgtgtg ataaatgatt atgcccgaaa
     2341 acaaaagctg atgaaagaaa atgacgacaa cttgaccggt gaagacgtgc gagaaggatt
     2401 gacagctgtt atttctatca agcatcctga ccctcaattc gaaggacaaa caaaaacaaa
     2461 attgggcaat tccgaagtac ggacagtcac tgatcggtta tttagcgaac atttcatgaa
     2521 attcttgctg gaaaacccaa gtgtgggccg gcaaattgtt gaaaaaggat tactagcttc
     2581 cagagctcgt ctagcagcga aacgtgcgcg tgaagtaacg agacgtaaag gtgctttaga
     2641 gattagtaat ctgccaggaa aattagcgga ctgctctagc aacgatcctg aaaaatgcga
     2701 attgttcatc gtcgaaggag attcggccgg cggatcagct aagcaaggtc gtaatcgaga
     2761 gtttcaagcc atcttgccta ttcgtgggaa aatcttgaat gttgaaaaag caagcatgga
     2821 caagatcttg gcaaatgaag agatccggtc tttatttact gctatgggta cagggtttgg
     2881 agaagatttt gatgtatcga aagctcgcta ccacaaatta gttatcatga ctgatgccga
     2941 tgtcgacggc gcgcatatcc gtacattgct gctgactctt ttttatcgtt acatgcgtcc
     3001 tattgttgaa gctggatacg tctatattgc tcagccacct ttatacgggg taaaacaagg
     3061 gaagaatatt acgtatgtac aaccaggaaa aaatgcagaa gaagagttga aacaagtcgt
     3121 tgcttcactt cctgcctcac caaaaccaag cgttcagcgt tataaaggac ttggagaaat
     3181 ggatgatcat caactatggg aaacaactat ggacccaagc aatcggatga tggcccgcgt
     3241 cagtgtggac gatgctattg cagcagatca gatatttgaa atgttgatgg gggatcgagt
     3301 agaaccccgt cgtgcattca tcgaggaaaa tgcacactat gtgaagaact tagatattta
     3361 atcatgaagg aggaaaaaga atgagtgaag aaatcagaga aaacatccat gatgtcaatc
     3421 taaccagcga aatgaaagac tccttcatcg attatgcgat gagtgttatt gtcgcacgtg
     3481 cacttcctga tgtcagagat ggtctgaaac ccgttcatcg ccgtatttta tacggcatga
     3541 acgaattagg tgtaacaccg gataaagccc ataaaaaatc tgccagaatc gtcggagatg
     3601 tcatgggtaa ataccatcct catggagaca ttgcgatcta tgagtcaatg gtacggatgg
     3661 cacagccatt cagctaccgt tatatgctag tggatggtca tggaaacttt ggttctgttg
     3721 acggtgacgg tgccgccgct atgcgttata ccgaagcacg gatgagcaaa atcgctacag
     3781 aaatgctgcg tgatatcaat aagaacactg ttgatttcca aagcaactat gatgatacag
     3841 aaaaagaacc agttgtacta cctgctcgct tcccgaatct tttagtgaac ggaacaacgg
     3901 ggatcgccgt aggtatggcc acgaatatcc cacctcataa cttgtctgag gtagtagcgg
     3961 ctatcgattt gctgatggat aatcctgatg tgacgaccaa tgaattgatg gaagtattac
     4021 ctggaccaga ttttccaaca ggtggtttgg ttatggggaa atcaggaatc cgaagagcgt
     4081 atgaaacagg taagggttct attaccgtac gtgcaaaagt tgagattact gagatgccaa
     4141 atggtaaaga acggatcatc gtgacagaat tgccttatat ggtcaataaa gcaaaattga
     4201 tcgaacggat ttctgaactg cacagagaca agcgtatcga agggatcaca gatttgcggg
     4261 acgaatcttc ccgtgaaggt atgcggatcg tcattgacat ccgtcgtgac gccagcgcat
     4321 ccgttatctt gaataactta tataaattga catccttgca aaattcattt ggattcaata
     4381 tgttggcgat cgaaaaaggt gtgccgaaag ttttgagctt gaaacaaatc cttgaaaact
     4441 atatcgaaca tcaacgcgaa gtcatcacac gacggacggt ctttgaaaaa gaaaaagcag
     4501 aagcaagagc acacatctta gaaggtttgc gtattgcttt ggatcatatc gatgaaatca
     4561 ttgcgattat ccgcgggtct aaatcagatg acgaggcaaa agcaacaatg atcgaacggt
     4621 tcgatttatc cgatcgccag tctcaagcta ttttagatat gcgtttacgc cgattgaccg
     4681 gtttggaacg ggaaaaaatc gaaaatgaat accaagaact cttaaaactg attgaagatt
     4741 taactgacat tctggcaaga ccagaacgtg ttacagaaat catcaagaat gaattagcag
     4801 acttgaatca acgatttggt gataaacgac gcacagaatt actagtcggt gaggtcttaa
     4861 gtctggaaga tgaagattta atcgaagaag aagaaatcgt tattacattg acaaacaatg
     4921 gttacatcaa acgtttggca aataatgaat tcagagctca acgaaggggt ggacgtggtg
     4981 tccaaggaat gggtatccac aatgacgact tcgtcaaaaa tctggtttct tgttctaccc
     5041 atgatacatt gttattcttt acgaataacg gaaaagtgta tcgggcaaaa ggatacgaaa
     5101 ttccagaata cgggcgtaca gccaaaggaa tcccaatcat caatttacta ggtatcgatt
     5161 cgagtgaaag catccaggca atcattgcgg tgacgggaga agcagaagaa ggccactatc
     5221 tcttctttac gacgcgtcaa ggtacagtaa aacgtacttc tgtcactgct tttgctaaca
     5281 tccgaagtaa tggtctgatc gcaattggtt tgaaagaaga cgatgagctc gtcaatgtgc
     5341 tactgacaga tggtcaatct aatatcatta ttggaacaca taacggctac tctgtcactt
     5401 tcaaagaaga agcagtccgc gatatgggac gtacagcttc cggggtcaga gggatacgcc
     5461 tgcgtgagga agattatgtc gttggggcag ccttaatgaa ggaaaatcaa gaagtattag
     5521 tgatcacaga aaaaggttac ggaaaacgga caaaagtatc ggaatatcca gtgaaaggcc
     5581 gcggaggtaa agggatcaaa acggcgaaca tcactgaaaa aaatggtcca ctagctggat
     5641 tgaccactgt ttctggagat gaagacatca tgctgattac agataaaggt gtcattatcc
     5701 gtttcaacgt atcgactgtt tctcaaactg gacgttcaac attaggcgta cgtttgatga
     5761 agatggaagc tgatacgaaa gttgtcacga tggcagcagt cgaaccagaa gctgcagaac
     5821 aaacagttga agaacaagga tcagaagaat aaccatcaag actaagtgtg taagctagca
     5881 agaataaccg attaaaaaat ctggttattc ctgctggctt ttttgattag ttgaatttct
     5941 cttaaaatat tcgaaactgt tcattctgta ttgcttttga ccaaaataga gtgtataatt
     6001 agagcttgtg agtagtctaa ttaattagat aactctcctt gctcttagga acctaagagc
     6061 cgaaagtcca cagggaggtg aatatcaatg gaaaacacga aatatgaaat catgtacatt
     6121 attcgtccaa acattgatga agaagcaaaa actgctttaa tcgaacgttt cgatgcaatc
     6181 ttgaaagata acggagcaga agttatcgaa tcaaaagatt gggaaaaacg ccgtttcgct
     6241 tatgaaatga acggttaccg tgaaggtatc tatcacatcg ttaaagtatc ttctccatca
     6301 tcagcaaatg ctgtcaatga atttgaccgt cttgctaaaa tcaacgacga tatcattcgt
     6361 cacatgattg ttaaagaaga agcttaattg ttgtttcatg tgaaacaata gaaaacgatg
     6421 agaggagatg actattttga ttaataatgt tgtattagtt ggacgtctga cgaaagatcc
     6481 agatttgcgt tacacagcaa gtggtacggc agtcgcaaca tttactttgg ctgtgaatcg
     6541 taattttaca aaccaaagcg gcaatcgcga ggctgatttt atcaactgtg tgatttggcg
     6601 caaatcagca gaaactttag caaactatgc tcgtaaagga acacttttag gtgttactgg
     6661 acgtatccaa actcgttctt atgataatca gcaaggacaa cgtgtatacg tgacagaagt
     6721 agtagctgac aacttccaat tgctggaaag tcgttctgca tctgaaaatc gccaacaaag
     6781 cggcggattc caaagttctg gtcaatcagc tggcggattt ggcggaaaca ataactctaa
     6841 tcaaacatct caatcatcca acggtatgcc tgatttcgac cgtgatactt ctgacccatt
     6901 cggctcaagt tcaacgatcg acatttcaga cgatgattta ccattctaaa cgaatttgat
     6961 aggagggaaa tagaatggca caacaaagaa gaggcggacg taaacgtcgt aaagtagact
     7021 acattgctgc taaccatatt gaatatatcg attataaaga tatcgaatta ctaaaaagat
     7081 ttatttcaga acgcggtaaa atcttgccac gtcgtgtaac tggtacaggc gctaaaaacc
     7141 aacgtaaaat tactgttgca atcaaacgcg ctcgtatcat gggcttgcta cctttcgtta
     7201 gcgaagacta ataaatccta ggaattgaat gactgatcct tttgatcagc tattcaattc
     7261 ctttttttct gtaaaaataa cctctattcg tttagtcgct cgaatgacga aagttatggt
     7321 aaaatcaaat tagtcatata tgggaggaaa attatggaaa ataaaaacaa tcatttatcc
     7381 ccgaaacctc tcttccttat cctgtttata ttaatcgaag tattggtagt cttaatttta
     7441 ccatttcgtt ttgttagcgc ttcggtcatt ctattgatga atttatgggg tgttcgctat
     7501 tggttgaaaa tgaaacgttg gcttatttta aataaacgtg aaaaaatcca gcaggcttcg
     7561 actgtagctg aaaccaatct agcttatgtc tcagaagtca tgcctgtagg agtgattgct
     7621 tatcagccat caagccaaaa aatcgaatgg ctcaatccat tagcattgaa aatcaaagat
     7681 caaagtggat taacagagga agaattgcta gatacttttt ttactgcaaa agaaaaaaag
     7741 aaaaaccaag tctatctagc agataaaaca tttcagtttg atatggatac agtaaagggt
     7801 gttgtttatt ttaatgatat tacccaaaac cagttgttaa aactccatca gacgaacagt
     7861 cagccggtga ttggtatttt atctattgat aactatgcag atgccattga taaattagat
     7921 gacaaagaga tttcttattt gaactctttc gtcactacac tagtttctga ctggatgaat
     7981 gaataccatg tctttttcaa aagaatcaac gcagaacggt ttttcttcgt agcacagatg
     8041 atcgatctga aaaagatgat ggataaaaaa tttgatttgc tggatcgctt tcgagcagaa
     8101 gctgaaaaac agagctcgcc tatcaccctt agtatgggga ttgcttatgg ctcaagaacc
     8161 ctcgatgaaa tcggtggaga agctcagaat aatttagatg ttgctttagt ccgtggaggc
     8221 gaccaagttg tcgtgaaaga ggtaaaagaa ggagcaaaac ctgtttatta tggggggaaa
     8281 tctgctaaga atgtcaaacg cacgcgtgtt cgttctcgag cgatgagtac cgctttgcgt
     8341 cggatttttg atgaaacagg cgatgtgttt atcatggggc acaagtttcc ggatatggat
     8401 gctttaggct ctgcttttgg tgtcagttgt ctggcacgct tcaaccagaa aaaagcttac
     8461 atcgttatca atgagaacga gatcattcca gatgtagaac ggtgtcttga agaaatccat
     8521 aagcatcctg aattagaaat gcaactaatc tctccagaac gtgccataga gctgaaaaaa
     8581 gaagaggacc tcctcgtgat ggtggattac cataaacctt ctctatcgat ctctcagggc
     8641 ctatatgagc aatttgataa gattgtgatc attgatcacc atcgtcgtgg ggaagagttt
     8701 ccaagtaaac ctctgctgac ttatattgaa tcttcagctt cttcagcttc agagctagtt
     8761 tctgagctga tccaatacca aagcagcaaa acaaatcgct tagatcgttt tgaggccaca
     8821 ttattgcttg cagggatggt tgtagataca aatagtttca atgtccgtac aactgcacga
     8881 acttttgacg ttgccagcta tttaaggtct tgtggagcag acgcatcctt agtacaatat
     8941 ttattgagct cggatttagc gacatacttg gagatcagca atttgattgc tggaagcgac
     9001 tatgtgactt ctgatattgt tgtggcggca gggacagacg aaaaagagta taatagtgtt
     9061 atagcagcaa aaacagcaga tacattgctt tcgatgatca atatcaatgc agctttcgtc
     9121 attacaaagc ggacagacgg cttgattggg atcagtgcca gaagtaccgg cacgatcaac
     9181 gtccaaatca tcatggaatc cttaggtggc ggaggacact tcacaaacgc agctactcaa
     9241 ctggaaaata cgacagttga ggaaacaaaa cagaagctat ttgctgcaat caaagaaaat
     9301 atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt aattttttta caagatgtaa
     9361 aaggaaaagg taaaaaaggg gacatcaaag aagttccagc aggttatgca caaaattatc
     9421 taatcaaaaa taacttggca aaagaagcaa cgaaaagcag tatcagtcag ttagcaggac
     9481 aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga agaagctaaa aagttaaaag
     9541 aattcttgga aaaagaagat actgtagtcg aattgaaggc gaaagcaggg gaagacagcc
     9601 gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg gttaaacaaa caatacaaaa
     9661 tcaaattaga taaacgaaaa atcgaattag aaaatccgat ccgttcacta ggctacacaa
     9721 acgtacctgt taagcttcat catgaggtga cggctacgat caaagttcat gtcgtacaag
     9781 aataacaaat cttgatagcg atgttcagct ggtaagtata accagaagga aaaaagacaa
     9841 accgaaattt atctgagaaa acagctgcag caatgaaagc aaaagctgca aatcgatttc
     9901 agattgattt cggtttcttt tttgggaaca gcaaaatagc ataaagaaaa cgagtataat
     9961 aaaagagaac tattttttta ttacactttc ggtacgaatg atgacttttc tctcattttc
    10021 ttgtaaaatg aagcaattga agacaaggag cgatacgtat gaatgaagtg tggcaggatc
    10081 gaatcccacc gcaaaatatt gaagctgaac aagcggtttt aggctctgtt tttcttgacg
    10141 cagaaacgat cattgatgcc atggagtaca tagagccaaa agacttttat cgtcgtggcc
    10201 atcaaattat ttttgagaca atgatcgagt taaatgatcg taatgaagcg attgacgtgg
    10261 ttactgtcaa agaccggctg gaacaagcca atttgttaga agatgccggt ggtttaagtt
    10321 atctctcaga cttggcatta gcagttccga cagcagcaaa tatcgtctac tatgcgaaaa
    10381 tcgttgaaga aaaatcattg ctgcgtacat tgatccagac agcaacagga atcgttacga
    10441 aaggattcga gcaaggagaa gaagttcaaa caattttaga tgatgccgaa cgcagtatct
    10501 tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc tattgctgaa gtactgaact
    10561 ccacgattga aaatatcgat cgactagctc aaaacaatga agaaatcaca ggtctaccaa
    10621 ctggctatca aggtctggat aaaatgacag caggtttcca gcccgaagaa ctaatcattc
    10681 ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt gaatattgct caaaatgtag
    10741 gaacaaaaac agatcgttct gttgcgatat ttagtttgga aatgggggca gaatctcttg
    10801 taaatcggat gctttgtgca gaaggttcga ttgaggccag ccatttacgt acaggtcagc
    10861 tttcggaaga agaatggtcg aatctgatcg ttgcgatggg gagtctttct cgagcaaata
    10921 tttttatcga tgatacacca gggatcaaaa tctctgaaat ccgtgcgaaa tgtcggaaac
    10981 tagcgcaaga aaaaggcaat ctaggcttga tattgatcga ttatttgcag ttgattgaag
    11041 gtaacggtaa agagaaccgt caacaagaag tatcagatat ctctcgccaa ctgaaaaaac
    11101 tggcgaaaga gctgaaagta cctgtcattg ctctttcaca gttatctcgt ggagtagaac
    11161 agcgacaaga caaacgacca gtgctaagtg atattcgtga atccggatca attgaacaag
    11221 atgcggacat cgtagccttc ttgtatcgag atgattatta tgaaagagaa ggcggagatg
    11281 acgatgatgg aaacgagcca aaaaatgaca acgtcatcga agtcatcatc gagaagaacc
    11341 gaagcggtgc acgaggaaca gttgaactac tgtttatcaa agaatacaat aagttttcgt
    11401 cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga gccttgagca atcccgagag
    11461 ctctcgtaag aagatgcaag gagtaaccgc aggaacgact agagtactta atttaaacag
    11521 atctccaaag ggattgtgac aaaagtcttg tcacagtccc tttttccgaa taaacggtgt
    11581 tcaatcagct gacccttggt attagctcag caaacgtcaa aatatgagga actattttga
    11641 cgtttgtttc tctaatactc aggtcaaaac gctgatctta cgacctcttt tccttaaacg
    11701 gtgttctcct gccaactcct cggtcacaag tcacaatatc atcaaacatg agaagcagtt
    11761 ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct ctttcttaaa
    11821 cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc aaacgaaaaa tatgagaagc
    11881 tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca acctctgatt
    11941 cacggtgttc caaaagtagc ttcttcgaaa aataagtcga aatttcacaa aaatttgaga
    12001 agcaattttc gtacattcct tcttatttct tgaagctgac cacttctgtt acaacctctt
    12061 ttttaataac cattctaaaa agataaagtt cgttttttaa gcgtttttat aaaataaaaa
    12121 taaaaatcat tcgattttat ttgaaatatg tatctattat tgatacaatg gttcaggatt
    12181 aaataatata gaacgaggtg ttcacatgtc atctgtagta gtagtaggta cgcaatgggg
    12241 cgatgaaggg aaaggaaaaa tcacagactt tctcagtgaa aatgctgaag tgattgcacg
    12301 gtaccaaggt ggcgacaatg cagggcacac aattaagttt aacggtgtga cttataaact
    12361 gcatttgatt ccttctggga ttttttataa agacaagatc agtgtgatcg gaaacggcgt
    12421 tgtagtcaat cctaaatcat tggttaaaga attagcttat ttacatgaaa aaggtgtaga
    12481 aacaagtaat ttgcgtattt ctgaccgcgc gcacgtaatc ttgccttatc atatcaaatt
    12541 ggaccaatta caagaagatg ctaagggcga aaataagatt ggtacaacga ttaaaggaat
    12601 cggacctgcc tatatggata aagcagctcg tgtaggtatc cgtattgcag acttattaga
    12661 taaagaaatt tttgaagaac gtcttcgttt gaatctagat gacaaaaacc gtcaatttgt
    12721 aaaaatgttt gattcagaac cacttgattt tgacgatatt tttgaagaat attatgaata
    12781 cggacaacaa atcaaacaat acgtcacaga tacttccgtt atcttgaatg atgctttgga
    12841 tgcaggcaaa cgcgtgttgt ttgaaggcgc acaaggtgta atgctggata tcgaccaggg
    12901 aacttatcct tttgttactt cttctaaccc agtagccggt ggggtaacaa tcggaagcgg
    12961 tgtagggcct tcaaaaatca ataaagttgt cggcgtctgc aaagcctata cgtcacgtgt
    13021 cggtgatgga ccatttccaa ctgaattatt tgatgaaaca ggagaacaaa tccgcgaagt
    13081 aggacgagaa tacggtacga caactggtcg tccgcgtcgt gtaggctggt ttgattctgt
    13141 agttatgcgc cattctaaac gagtttcagg tattaccaac ctatctttaa actcgatcga
    13201 tgtattaagt ggattggaaa cagtaaaaat ttgtacggct tatgaattag atggcgaact
    13261 aatctaccac tatccagcaa gcttgaaaga gttgaaccgc tgcaaaccaa tctatgaaga
    13321 attgccaggc tggtcagaag acattacagg atgcaaaaca cttgcagaac tgccagaaaa
    13381 cgcacgtaac tatgtacgtc gtatctcaga gcttgttggt gtacgtatct caacattttc
    13441 tgtcggacct gatagaacac aaacaaacat cttagaaagc gtctgggcgc aaatctaagg
    13501 ataaaaacga cgactgaatc aggacgttct taccagagcg agtcgaagtg tttatctata
    13561 cgataataat aacttgaaga ggtagtgaaa gagacgttta gccttattgc cgataggctg
    13621 tctcttgaac actgtttatt cagaataagg gactgtgaca aaaac
//
LOCUS       DAYYCW010000076        13263 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000076 DAYYCW010000000
VERSION     DAYYCW010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.078"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..2376
                     /locus_tag="ACHAYA_002047"
     CDS             4..2376
                     /locus_tag="ACHAYA_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM8605835.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="ACHAYA_002048"
     CDS             2437..3897
                     /locus_tag="ACHAYA_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM8605836.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="ACHAYA_002049"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="ACHAYA_002049"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8605837.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="ACHAYA_002050"
     CDS             6128..6277
                     /locus_tag="ACHAYA_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605838.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6352..6945
                     /locus_tag="ACHAYA_002051"
     CDS             6352..6945
                     /locus_tag="ACHAYA_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM8605839.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6958..7857
                     /locus_tag="ACHAYA_002052"
     CDS             6958..7857
                     /locus_tag="ACHAYA_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM8605840.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7860..8357
                     /gene="ssb"
                     /locus_tag="ACHAYA_002053"
     CDS             7860..8357
                     /gene="ssb"
                     /locus_tag="ACHAYA_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8605841.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8723..8983
                     /locus_tag="ACHAYA_002054"
     CDS             8723..8983
                     /locus_tag="ACHAYA_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605842.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9428..9634
                     /locus_tag="ACHAYA_002055"
     CDS             9428..9634
                     /locus_tag="ACHAYA_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM8605843.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9655..9885
                     /locus_tag="ACHAYA_002056"
     CDS             9655..9885
                     /locus_tag="ACHAYA_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605844.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9901..10338
                     /locus_tag="ACHAYA_002057"
     CDS             9901..10338
                     /locus_tag="ACHAYA_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605845.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10331..11038
                     /locus_tag="ACHAYA_002058"
     CDS             10331..11038
                     /locus_tag="ACHAYA_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605846.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11202..11402
                     /locus_tag="ACHAYA_002059"
     CDS             11202..11402
                     /locus_tag="ACHAYA_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605847.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11418..11597
                     /locus_tag="ACHAYA_002060"
     CDS             11418..11597
                     /locus_tag="ACHAYA_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605848.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11922..12185
                     /locus_tag="ACHAYA_002061"
     CDS             11922..12185
                     /locus_tag="ACHAYA_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFM8605849.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12179..12529
                     /locus_tag="ACHAYA_002062"
     CDS             12179..12529
                     /locus_tag="ACHAYA_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8605850.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12553..13167)
                     /locus_tag="ACHAYA_002063"
     CDS             complement(12553..13167)
                     /locus_tag="ACHAYA_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8605851.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         4904 a         2194 c         2729 g         3436 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctaga
      661 ccaactgatt ctatgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacattgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaggaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaaccaat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag
     6361 aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa
     6421 ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt
     6481 gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa
     6541 cgtttttcca ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg
     6601 aatatgccga aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt
     6661 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
     6721 gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
     6781 tgtgatattt ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa
     6841 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
     6901 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
     6961 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
     7021 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
     7081 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
     7141 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
     7201 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
     7261 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
     7321 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
     7381 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag
     7441 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
     7501 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
     7561 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
     7621 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     7681 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     7741 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     7801 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     7861 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     7921 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     7981 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     8041 tggtgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     8101 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     8161 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     8221 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     8281 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     8341 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     8401 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     8461 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     8521 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     8581 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     8641 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     8701 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     8761 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     8821 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     8881 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     8941 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     9001 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc
     9061 tttttaggaa ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc
     9121 gccgttcgag aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa
     9181 gcaagacaaa atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct
     9241 ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     9301 gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca
     9361 acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga
     9421 atcaatcatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
     9481 ctatcatgca ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag
     9541 ctatcgtttg tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta
     9601 tattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact
     9661 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
     9721 gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
     9781 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
     9841 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
     9901 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     9961 atggaggata tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa
    10021 agattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    10081 acgtactatc gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc
    10141 ttactatgct tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct
    10201 ttagaactaa cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    10261 tggttagatt attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag
    10321 gacaacgaaa atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt
    10381 aacaaacaaa gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat
    10441 ggaagaaatg aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac
    10501 gagtcaaacg aaggtcacag tcagcaataa ttatttagac gtgtataaac atcgtcctga
    10561 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    10621 ttacctagaa aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca
    10681 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    10741 aatggaagag ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag
    10801 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    10861 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    10921 cacgacttta actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa
    10981 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc
    11041 atttataccg aaaagtgaga atgtgtgaaa taagaacaaa aaccttgaaa ttaagggaat
    11101 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag
    11161 cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc
    11221 tgttgttcgt actgtgtatt ggcgagatac ttatgcgtca tggtgcagct gtacatttaa
    11281 acttcgtagt attcttagga atactagtat tttgtttgat tttttggcgc tttgtgttag
    11341 cccttgtgag tctactattc aagttgattg cagccatatt tttcataggg ttatttcgat
    11401 gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc gtgacattag
    11461 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa agtgaatcta
    11521 agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatatac
    11581 cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata gcacttaatt
    11641 gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat caataactat
    11701 gaaatcttta aagtatccag atcaattttt ttcttacctg attctaaaga ttttgttttc
    11761 ttaaacatat tatgatttcc tttcgactat tttcatataa atgttaacat agtttatgga
    11821 ttcatggaat gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt
    11881 atgtatgtta tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa
    11941 aaaaatgggg aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa
    12001 ttcatacaga agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa
    12061 ttatcgaagc ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca
    12121 ctgatgaaag aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat
    12181 ggtgagaaaa cctagacaag gcgatattct attattgaat acagcacctc gatctggaca
    12241 cgaacagaca ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag
    12301 taacgttgtt acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa
    12361 tataaactca gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac
    12421 aattgattat gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga
    12481 ggagttattg atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag
    12541 cagcatgatc aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg
    12601 tctcgtaaca ttgtagtctt ttgcaatctt agcgatcgca acaccttttt ttagatcctc
    12661 aacgatgttt ttataaacaa gacgacgttg gggatctttt gcattggcac tgtatagctt
    12721 aggacgtcct ttataaacac cgttcgcttt tgcaatctta atgccttgtg cctgacgtcg
    12781 tttagattcg gtacgttctt gttcagcgat catggccaaa atttgaatga tcaaatcttt
    12841 aatgaattta tccaggagag gattgccaat tgcttcggcc atgatcggta gacttgtgat
    12901 gatcagctgc acatttttct tcttcaagta attgactgaa gcgatgatct cgtcgtaatt
    12961 tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga cgaaatccag
    13021 tgcttcttga aaaacaggtc gatgagtaat cgttgcgcca gattgtttct caatgaaaat
    13081 tctctctgca ccgaattttt tcatttcttc aatctgccgt tgttcatttt gatcgatgga
    13141 tgagacacgt atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaaa
    13201 acaccctaat catacacgag aaaaccagat ttttcaatgt cgcggtcgtg tctagttagg
    13261 gta
//
LOCUS       DAYYCW010000077        13086 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000077 DAYYCW010000000
VERSION     DAYYCW010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.079"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2199)
                     /locus_tag="ACHAYA_002064"
     CDS             complement(79..2199)
                     /locus_tag="ACHAYA_002064"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8605852.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="ACHAYA_002065"
     CDS             2429..3607
                     /locus_tag="ACHAYA_002065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM8605853.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="ACHAYA_002066"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="ACHAYA_002066"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM8605854.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="ACHAYA_002067"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="ACHAYA_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM8605855.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="ACHAYA_002068"
     CDS             complement(4960..6213)
                     /locus_tag="ACHAYA_002068"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8605856.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="ACHAYA_002069"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="ACHAYA_002069"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM8605857.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="ACHAYA_002070"
     CDS             7790..9136
                     /locus_tag="ACHAYA_002070"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM8605858.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="ACHAYA_002071"
     CDS             complement(9444..9830)
                     /locus_tag="ACHAYA_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8605859.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="ACHAYA_002072"
     CDS             complement(10098..10247)
                     /locus_tag="ACHAYA_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605860.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10709..10921
                     /locus_tag="ACHAYA_002073"
     CDS             10709..10921
                     /locus_tag="ACHAYA_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605861.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="ACHAYA_002074"
     CDS             complement(11199..12998)
                     /locus_tag="ACHAYA_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM8605862.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2354 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 tttaac
//
LOCUS       DAYYCW010000078        13062 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000078 DAYYCW010000000
VERSION     DAYYCW010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.080"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..188
                     /locus_tag="ACHAYA_002075"
     CDS             <1..188
                     /locus_tag="ACHAYA_002075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605863.1"
                     /translation="QTRLFLAGISFLKELKREKMIFIIKEFLSFSLEKISVTMENRAK
                     KNQSFSLYIILPIYRNC"
     gene            220..1122
                     /locus_tag="ACHAYA_002076"
     CDS             220..1122
                     /locus_tag="ACHAYA_002076"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM8605864.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            1335..3110
                     /locus_tag="ACHAYA_002077"
     CDS             1335..3110
                     /locus_tag="ACHAYA_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM8605865.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(3176..4762)
                     /locus_tag="ACHAYA_002078"
     CDS             complement(3176..4762)
                     /locus_tag="ACHAYA_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM8605866.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            5013..5237
                     /locus_tag="ACHAYA_002079"
     CDS             5013..5237
                     /locus_tag="ACHAYA_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM8605867.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            5378..7129
                     /locus_tag="ACHAYA_002080"
     CDS             5378..7129
                     /locus_tag="ACHAYA_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM8605868.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            7129..8913
                     /locus_tag="ACHAYA_002081"
     CDS             7129..8913
                     /locus_tag="ACHAYA_002081"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8605869.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            9239..10090
                     /gene="licT"
                     /locus_tag="ACHAYA_002082"
     CDS             9239..10090
                     /gene="licT"
                     /locus_tag="ACHAYA_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM8605870.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            10186..12066
                     /locus_tag="ACHAYA_002083"
     CDS             10186..12066
                     /locus_tag="ACHAYA_002083"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8605871.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(12106..13008)
                     /locus_tag="ACHAYA_002084"
     CDS             complement(12106..13008)
                     /locus_tag="ACHAYA_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8605872.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT         4142 a         2322 c         2760 g         3838 t
ORIGIN      
        1 aacagacaag gctgtttttg gcaggaattt cttttttaaa agagttaaaa cgtgagaaaa
       61 tgatctttat tataaaagag ttcttaagct tttctttaga gaaaatttca gttactatgg
      121 agaacagggc aaagaaaaat caatcatttt ctttgtatat catcttacca atatatagga
      181 attgttagta acaattgcct ttcaaggagg aatccatcca tgttattagg ctcacacgtc
      241 agcatgagcg gaaagaaaat gctgttagga gcagcagcag aagcagccag ttatggatct
      301 tcgactttta tgatctatac aggcgcccct caaaacacta gaagaaaagc catcgaagag
      361 atgaatattg atgccggaaa agcgtacatg gcagaaaacg gtctaacaaa tatcgtcgtt
      421 catgcccctt atatcattaa tttggggaat acgatcaaac cagaaatgtt tccttttgcc
      481 gtccaatttc tgagagaaga gattgttcgt gcggaagctt taggcgctac tcaaattaca
      541 cttcacccag gtgctcatgt aggggcagga acagaagccg gaattgctag aattattgaa
      601 ggcttgaatg aagttttgac aaaagaacag acggcacaaa tcgctttgga gacaatggca
      661 ggtaaaggta cggaaatcgg tcgaacattt gaggaattag cgcaaatcat cgatggagta
      721 aaattaaatg aaaaattatc cgttactttt gacacctgtc atacgaatga tgccggttat
      781 aatattaaag aagacttttc aggtgtaatg gaagagtttg ataagatcat cgggttagat
      841 cggcttaaag tactccatat caatgattct aaaaatccgc aaggttcgca taaagaccgg
      901 cacgccaata ttggttttgg aacaatcgga ttcgacacgc tgaataagat cgtccatgat
      961 ccaatgtttg acaatatttc gaaaatcttg gaaactccct atgtcggcaa agataaaaag
     1021 aaagcttacg caccatatgg aaaagaaatc gccatgttca aagcacaaat attcgatcca
     1081 gaagtgtttc ctgagttatt agcagaagaa gtgatactct aagattttct atctgacagc
     1141 agcaaaaaag gaccaggcac attgccttgt ccttttttgt ttaagaaaca aaggagtact
     1201 ttttgcagtc tttttcatca tgcatcttct cccccctctt tttaaattta gacaaacaag
     1261 gtatactgtt agaaaagtaa aaaaacgaaa agtttcacaa gcaagtggca aaacgtgatt
     1321 ggaaggacga aaagatgctg aacactttaa aacccttgat caaccaacta aaaaagaaac
     1381 atacatactt tattcttggc atagccctat tatttacaat tattcgtttg tatttatata
     1441 gtaaagcaat ctatggaaca gacatgtcgg caacctatga tgaccagctg ctgattcgct
     1501 atgctcaaaa tattttggac ggtaaatggc taggggagta tacgactaca acactttcca
     1561 aaggaatctc ctacagctta tttttagtac ttgcacataa actgcatctt tcttatgctg
     1621 ttttgttcgg catgttcagt atttttgcca gtatattggt tgcactcgcg tttaaaccga
     1681 tcgtgaagaa caagtatctt cttttaagta tctattttta ttttttgttt tctccgatta
     1741 gttttacaca tgaatattcc actcgaacat atcgtaatgc cctcgtgatt ccggctgttg
     1801 agatcattat cgcttgttta ctagctattt attaccgcaa atacggttct agcagaaaat
     1861 tgctgccatg gtttttaggg ctttctgtcg cttttccttt cttttggttc attcgcgaag
     1921 attctatctg gcttcttcct tttactgtag tagctttggg tatcagtttc gtccagatct
     1981 tgctagggca agacttttct ttcaaagaag taaagaagtt aaaaaaacga ctctttcttc
     2041 tctcgaaacg ggaatggctg aaactttcat tgtttttact tcctatagtt ggcttttcca
     2101 tcgtctatca gtggatcgaa tataaaaatg agacaacata cggtattgca accatcaatg
     2161 accgttccag tggagaattt ggacgtttga tgaaacaact gatccgaata gatgatgaaa
     2221 cgaacttgaa taaagaaaac agtgagatat gggtctcaca tgctgcacta aataaagcat
     2281 tagccgcatc tcctacattc catactgttt ctacccgtat tgacgaatta tatacgactc
     2341 acgcgtggac tcaaggtgga aaagtaaaag aattacccgg agatatcatc ttttgggcat
     2401 tgcgagatgt ggtaagcgaa tctggttatt accaaaacaa tgcaaaagaa accaatgatt
     2461 tttggaaaaa agtcaaccaa gaattagaag aagcctatca caagggaacc atcaaaaaga
     2521 aaaaagagtt ctatctgatg ggatcaggag atggaaagac gatgagtgat ctgccaatcg
     2581 tattcgattt ccttcaatca ggatacgctt atgcatggcg atataaagat ttccatcaag
     2641 gaggaaatta tagtttcgga acgcaagaac aagtcaaaca ggctcaagaa ttattaaacc
     2701 ttccgctaat ggagaattgg atcgatagta atgaaccgag aacacctaat ttccaattaa
     2761 caaaatcagc aaaagtcgcg aatgtcatca taaaaattta ccaagttctg gctattcccg
     2821 tgctgattgc ttcaatagca gggatattac ttatgatact aggaaccgta tttgcaaaag
     2881 ctgatcgtgc ctattatagt tcctctttga ttgtggtcac cggattaata ctgacacaat
     2941 tggtttttct tttcggtgtc tcgtggtttt gttcttatgc gccagaaaaa aaggattatt
     3001 ttttaagtgt ctacactggc gcaggcgtgc cattaacgca attcatcgta gtaacaagca
     3061 tgctagtttt cagtaaatta aatctgaaaa aggctttatc ttctaaataa aaagaccctt
     3121 ggtagctaga tttttaacct aactatcaag ggtctttgtg tggaaaaatc agtttctatt
     3181 cgatttcttc taccgtttcc tgcaaacgct gttcatattt tttgtttcct ttatataaat
     3241 caccaatcgt ccatgccaac aggagaagga caagaacaat gattacataa gcgatgtgat
     3301 cagccaatgc tagttgagca gaagtggctt gttcaccaaa gaaactttcg atctgtcctg
     3361 gtaatcctcg taagttcaag taagttagac taatcacaga gaaccagcca agaagtttta
     3421 cgatgaatga atttttaaat cggctaccca tctccgcttg gctgttggtc atcatcagta
     3481 aagggatcat cgaaaacggc aacgcaaaag ccaagaacac ctgggattca ttcatcaagt
     3541 tattcaaagc ggtatgttct tcgatcgttc cttttgtact tgtaaacaaa acacaaatca
     3601 atacaggaat cacagaaagc aatcgtgtga ccagtcttct tagccaaatc gggatacgca
     3661 tatggatgaa gccttccatg atgacttgac cggtcaacgt accagtaata gtagagttct
     3721 ggccagaagc aagaagtgct acagcaaata aagtggataa gataccagat tttgctacag
     3781 caatcaagat accattgctc atagctgatg gatcagataa ggcttcatac agaccaaaaa
     3841 aggaaggatc ttggacagaa ccggttttga agacagcgac acccatgatc aatagcagtg
     3901 cattgactag aaaagccatc gacaattgga tattagagtc ccaagtagaa aaacgtacag
     3961 cttgtgctac atcttcttcg tcttggtgat caatcttccg agtttgagaa actgctgaat
     4021 gaagatataa gttatgcggc atgactgttg caccaataat ccctaaagct ccagtcagcg
     4081 gagtttcgcc gccgatgacc ggtgagtctg agaatgtatg actgttaggg ataaagccag
     4141 caaataaact gtgccaatct gggttagaca agccaacttg atagacaaaa acgaataaaa
     4201 tgacgaaaat taagcagaca accagtgctt cgatcttacg aaacccgact tttgtcagta
     4261 gtaagagaac gaaaacatct aatacagtaa taaagacggc aatcaccaat ggaatatcaa
     4321 aaagtaagta gagggcgatt gctgcaccaa tgacttctgc gatgtctgtt gccataatgg
     4381 ctaattcagt caagatccat aaaatgacac ccaatgtctt acttgtgcgt gctcggattg
     4441 cttgggccaa atccatttgg gtcacgattc ctaattttgc tgccatatac tgtaaaagca
     4501 tagcaatcag actagacatc aaaataactg acatcaataa atattggaaa ttttgtccac
     4561 cagtgatcga agtggaccag ttacctggat ccatataacc aactgctact aatgcacctg
     4621 gtccagagta agcaagaagt gttttaaaaa aacttttccc ctttggtaca tcaactgttc
     4681 catttacttc agctaaagat aaaccatttg tatgttcaat aagtcgatgc ttatgaaatt
     4741 gtttgttgga ttgattattc aagaaaaaac cacctttcct atttatctac gattacattc
     4801 tacacctcaa aaagaaaaaa ttaaagaaaa atattcggta tgcctaattt attttcgtgg
     4861 cgcgtcttgt tagatgacga aagaaatttt gttctcctct aaaaaaacca ctattttttg
     4921 catgaaacta aagaatacgc ctttcttttt tcaataaact ttagtaaaat aagtcattga
     4981 gagcaattcg gaaatagaaa ggagagattt atatgttatc aacaggtatt gtcgtactta
     5041 ttgctattat tgctttgcta atcggcgcag taggtggctt tttcctagcg cgtaaatata
     5101 tgaaagacta tttagaaaaa aatcctcccg ttaatgagga catgttgcga tcaatgatga
     5161 tgtcaatggg acaaaaacca tctgagaaga aaattcgcca gatgatgcag caaatgaaga
     5221 accaagggaa aaaataggtt ctgacccaac gactttttac gggctgattt tttcagtccg
     5281 ttttttattt tgtaatttgt tagactattc agattattca gcaaaaagac aagcacagac
     5341 aatcctaggc tcttgataaa gacaaaggag gagtaaaatg tcgattttta gaaagttagg
     5401 ctggtttttt cgtcaggaaa agaaacatta tttaattggg gttttctcgc tattcgcagt
     5461 ggccttggta cagttagttc ctccaaaagt gattgggatc atcattgatg agattgccga
     5521 taaaaattta tcagtaaaga tcattttcgg gtggatcttc ctattgatta tcgctgcttt
     5581 cttacaatat ctttttcgtt atatctggcg gacgaatatt tggggaagtg cagctcggtt
     5641 ggagaaagaa cttcggcagc agttattcaa acattttaca aaaatggaca atctctttta
     5701 ccaaaaatat cggactgggg atttgatggc tcatgcaacg aatgatttga atgcgatcca
     5761 gaatgtagca ggagctggta tcttgacctt tgcagattca gtgattacgg gcggaacaac
     5821 gatcattgtg atgattttgt ttattgattg gcgcttaaca ctgattgcgc tgattcctct
     5881 gcctttgctg gccgtcactt cacgagtgct ggggtcgaaa ctccatgatg cttttcgtga
     5941 ttcgcaagaa gctttttcta gtatcaatga taagacacaa gaaagtatca ctgggatcaa
     6001 agtcattaaa acatttggac aagaaaaaga agatctagcg gattttgaag aaaagattga
     6061 agatgcaatt attaaaaaca aacgagtcaa ttttctagac tccttatttg atccattcat
     6121 taccttgatt atcggtctgt cttatgttgc aacaattatt ttaggtggaa agtatgtctt
     6181 ggaaggcacg atctcgatcg ggcagatggt ctcatttatt agttatatcg gtatgcttgt
     6241 ttggccgatg tttgcgattg gtcgattgtt caacgtattg gaaagaggaa atgcttctta
     6301 tgatcgcgtc aatgaacttt tgcatgaaaa aacgcatatc gtggaacaac tgggtgcagt
     6361 gcagaccccg gtaaaaggcg agctgaatgt agctatttcc tcttttaatt atccgaaaga
     6421 agaatcacca gcattagaac aaatcaagtt tgcaatccaa gaaggagaaa cattaggtat
     6481 tgttgggaaa actggtgcgg gaaaaacaac gatcatcaaa ttactgatgc gtgaatacga
     6541 tgattatcaa gggaaaatcc aatttggcgg acgttcgatc aaagaatatt cattgaatgc
     6601 tttattagga tcaatcggct atgtgcctca ggatcacttc ttgttttcga tgaccattcg
     6661 agataatatc cgtttttcta atccaaaact ttcagaagaa gaagtagagc atgcggcttc
     6721 tttagcattt atcaacaatg aaatcaagga attaccaaaa ggttatgata ccttagttgg
     6781 agaacgtgga gtctctttat ctggcggaca gaaacaaagg atttcgattg ctcgagcatt
     6841 gatcaaaaat cctgaattat taatattaga tgatgcactt tctgcagtag atgccaaaac
     6901 agaagaagct atcttgcata atttaaaaga aataagaaaa gagaaaacaa ctatcattgc
     6961 agctcaccgt ttaagtagcg tgatgcatgc aaaagaaatc attgttgtcg atcaagggaa
     7021 aatcattgaa cgaggaaccc atcatgagct gcttgcacta ggcggctggt atgaacaaat
     7081 gtgggagaaa cagcaattag aagcgaagat agaaggaggc gaggcctgat ggatgaaaat
     7141 tatcaatcgg aatggacaaa atcgattcca cttaaagaac agaaaaagat catcaaacga
     7201 ctgatgaagt tcgccaaacc ttttagaaca acgttcatcg ttgctatctt atttgcgttt
     7261 gctctgtctg tgatcaatgt cctattgcca aggatcatcc aaacttttat ggatgatcat
     7321 ctggctaaac agtcggctac gacacaagtt attttgtttt ttgctggtat gtacttattt
     7381 ggagtcctag taaaaagtat catttggttt ttccaatggt atttgtattc catggcttca
     7441 ttgaagacct atcagtatat tagggtaaaa ttatttgaaa agctgcatac actaggtatg
     7501 cgttattttg atcagacacc ggcaggatca accgtttctc gagtgacgaa tgacacagaa
     7561 acgctatttg aattttggta cgtctttttg atggtattga ctgggatctt tgcagtcgtt
     7621 tcttcattca ttgccatgtt tcaaatcaat ggagaaattg ccttgtggac tttggtgttc
     7681 ttgccgattt taggaattgt catctggtat taccagaaat ttagttctcg tatttaccga
     7741 aatatgcggg aaaaattaag tcagctgaat acaaaattga atgaatcgat ttccggcatg
     7801 cgtattatcc agcagtttcg ccaagaagct cgtttagcta aagaatttga acaagtgaac
     7861 aatgagtatt tagacacgcg ctatgcgatg atcaaaacga attcgattct gttaagtccg
     7921 atcatcaatt ttctatatgc attggcgatt gcgttggcgt tgactttatt tgggatcgat
     7981 gcgttgcatt ctcccgtaga agttggactg atctatgcat ttacgactta tgtccaggga
     8041 ttttttaatc caatgtctca aatgatggac tttttgagca tcttcacaga tgggatggtt
     8101 gctggtagtc gtatcttgaa gattatggat acggaagaat atgcaccaca acaaaaccct
     8161 ggagcagatc aaatgattac ttcaggtaag attgagtttc gcaatgtaag cttttcttat
     8221 gatggagagc acaatgtatt aaacaatatc tcttttgttg ctaatccggg agaaaccgtt
     8281 gcattagtgg gacacaccgg aagtgggaaa agctcgatca tcaatgtgtt gatgcggttt
     8341 tacgaatttt atgaaggtca gatattgatt gatgatcatg acattcgaga atatccaatg
     8401 gaagagttac gtaagaaaat ggggctggtc ttgcaagatg ctttcttgtt ttatggggat
     8461 atcgcaggaa atattcggat gatgaatcca gctatcacag acgaacagat acgccatgcg
     8521 gctgagtttg ttcaagcaaa caaattcatc gatgaattac caaaaggata tcactcaaaa
     8581 gtgatcgaac atggtgccag ctattctagt ggacaacgac agctgatcac ttttgcgcga
     8641 acaatcgtga ctgatcctaa aattctagta ttggatgaag caacagcaaa tatcgatact
     8701 gaaacagaag gattgatcca agaaggattg gcgaaaatgc gacaaggacg gacaacgatt
     8761 gcgattgccc atcggctgtc aacgattcga gatgcagaat tgattttagt tttagacaaa
     8821 ggtcatatag tagaacgcgg gaatcatgaa acacttctag cgcaaaatgg tttgtatgca
     8881 gatatgtaca aactgcaaag tagtggtata taaaagatgt tgtgacagta gcattttgac
     8941 ctaagcacta gagtgaaaat aaaacatagc ttcccatatt tttcatctgt gagagttagt
     9001 gtcgtagtta ccagatttaa aaaagacgac gtcagctagt cagaagtgcc taatagcccc
     9061 taagaaagcg ttgacaaaat cgtttgcttt cgataatata aagaaaacga tacaacttgg
     9121 atagtgattg cctgatgcaa gctaaacctg atgagtctcc catggtttca tggacggctt
     9181 tttgggtttt tctttttgtt ttggatacag ccctatatat catggaaagg agcaaaacat
     9241 gctgattgag aaagtactga ataacaacgt cgtaataaca agggatgaaa gagatcggga
     9301 aatgattgtg atgggaaaag gattggcatt caaaaagaag gtgggtgacc cgattcttga
     9361 ctcatgcatc gacaaaaaat ttcagttgac aaatgacgga ctttcacaac agttccaaga
     9421 attggcacaa gcgattcctc ttgagtactt agaaatttct tgtgaaatca ttgaatatac
     9481 aaaagaggct ctgcaagtcc atttaaatga ttctgtgtat atttcattga cggatcactt
     9541 gtatacagct atcgaacgtc aaaaaatggg gatctttgtg ccaaatgtat tgctgtatga
     9601 tatcaaacaa ctgtttccta aagagtatgc cattgggaaa aaaacagtga agaaaatctc
     9661 ggataaatac cacatcaagt tatcagaaga cgaagccgga tttatcgcat tgcatttagt
     9721 caatgcacaa ggcattcaaa gccagcaaga caatacctac ctgtttacca caacaataca
     9781 ggatatttta aatatcgtcc gctattacta taagattaca tttgatgaag attcagtcca
     9841 cttttttcga ttcgtcacac acctgcgttt ttttctgaca cgtgtcctag atggagcgaa
     9901 atcagaagat ggagaagttt ctgaggaatt gttccaagtt attcaaaaaa aatataagga
     9961 tacgtttggt tgcgttgaga aaatagcaga ctttttaacc ggggctttcg actaccagat
    10021 gtccacggat gaaaaaatgt atttaacgat ccatactgcg cgtttagtgc atgagaatcg
    10081 ttgtaaataa ataaaaaagg aggacaaatt aacaagaacg tatgaaataa gggtagtgac
    10141 attcagttgg ctaaaccttc cactaattaa ttggagggaa tagaaatggg aaaataccag
    10201 gaattagcag aaaacatcgt aaagaatatc ggaggaaaag aaaatatccg tagcttgaca
    10261 cactgtgtga cgcgacttcg tttcaaacta aaggaggaaa ataaagcaaa ggatgatgtt
    10321 ttgaaaaaga tggaagggat agtcaccatc atgaaaagcg gcgggcaata tcaagttgta
    10381 atcgggaatc atgtgccaga tgtctatgca gaagtactga cagtagccgg gatttccgat
    10441 gaagcagaag aagaggtttc ttcaagtagt aatctgttca atcgattaat cgacatatta
    10501 agtggctgtt tccagccttt tttaggtgct ttggctgcag cgggaatggt caaaggatta
    10561 aatgctttgt tcttgtttct caaattttat actgaaggat cgggtacgta catcatgctg
    10621 aatgggatag gggatgcaat tttttatttc atgccagtga tcttagggta cacagcagcg
    10681 aaaaaatttc atctgaatcc gatggtgggg ctcgtgattg gtgcagcact ttgttatccg
    10741 actgtacaag gagcagcact acaaagtggt ttccctgatg gcagtcctgc gccttatagc
    10801 ttgctgggtc ttccggctta tgaaacattt ttgggtatcc catgggtggg cagcagttat
    10861 actagtagcg tcgtacctgt tatcttcatt atcgcatttg ccggacaagt ccaaaaaatt
    10921 gccaagaaat ggattcctgc agtcgtccag acctttttgg tttctttttc tgtgttatta
    10981 atcgcacttc ctgctggctt tttgattatc ggtccgattg tcagtatact gacagatcta
    11041 ttaagcacgg gattccaagc attgatggac ttttctccag ctgtgtatgg tgcagttcta
    11101 gggtttcttt ggcaagtact ggtgattttc ggacttcatt ggagtattat ccctcttgcg
    11161 attatgcagc tagcgcaaaa tgggtggagt cagatgttag taggcgttta tggcgcgagt
    11221 tttgcacaga cagcagtggt attggcgatg tatttcaagt tcaaggatca aaaaatgaag
    11281 gcgctttgca tgccagctgt gatttcagga atcttcggcg taacagaacc ggctatttat
    11341 ggaatcacct taccgaagaa gaagccattt atttactcga tgattggtgg tgctgtttca
    11401 ggaatctatt tgatggcatc tggaacaatc agctacataa tgggcggatt agggattttt
    11461 ggggtggtaa actacatcac ggaaacagac gccagtggta tgtttcaagc gtttatcggc
    11521 attgctattg cttcggtaat cgggttttcg ctgacttttt tcttttggaa ggatgaagca
    11581 ggtgaaacag atgctataca gaaaaaatca ggtaaagaaa ttgtaaaaac accgatacaa
    11641 ggacaaatcg ctccactaaa tgcagcgaag gatgcagctt ttgcacaagg aacactaggt
    11701 cgaggaatct taatctaccc tgaaaaagga gaagtacgtg ccccttttga cggaacagtt
    11761 atgaccctgt tcccgacgaa acatgcaatc ggaatggttt ctgaaacagg gctggaattg
    11821 ctgattcatg tcggattgga tactgtgcag ttggaaggga aatattttga aagcttagtg
    11881 cagcaaggag aaagagtgag acaaggcgat ctgttgctac gcttcgacaa aaagaaaatc
    11941 gaaaaggaag gatacagttt agaaacacct gtgatcgtca ccaattacac ggattatttg
    12001 gatattttgg aaaaccaagg ggagacggtc gggccagacg atgcacttat caccgcactg
    12061 acctgaacgg ttaggtgaag aatggactag gcttaactat tctttttatt ccacttcttt
    12121 attttcaaaa ttgaaaggct ctttcatgat ggctgagctg atagggcgtt tatcgaattt
    12181 ttgaccgaca gaaattagat ggactaaatt ctgttgataa ttttctgcaa aaacacaact
    12241 atataacacg tctagaagat aacaaattga tgtattgatc gtaaaattac caatttttga
    12301 atacagtcgc tcgcgtgtcg taagtcgcaa agcggctgta ctcattgcag atagcgttga
    12361 atcaccgata ctggtcaaag ctaaaattgg aacatcattt tgtttcaata tttctgcggt
    12421 acgaaggatc acattgtttt cacccgtata agaaatcaga atcccgcaca tatcgctcgt
    12481 acaattgtac gcttcataga atgattcacc aaaagtggtg acaattgtga catccatttt
    12541 gatgcgcttc attttcagca tgaaatcttg agaaatcaaa gtattcgagt tactggcgaa
    12601 gatcacgatt ttttttgcct ggagcagtaa atatttagct ttttgcagtt ggtcatgatg
    12661 aatgagagaa agtgtatcat gaatcgtcat ctgttctaaa gaagccattt tatttgcgat
    12721 tgtcataata ccgtctgtgg tggaaaaagg aagattcgca tctacgtctt gaaaattccc
    12781 ttcaagatag gctgtttcag ctaaaaaagc attacgaaaa ctgacccagc catcaaaacc
    12841 catttttttt gctacacgga tgagtgtaga cgggtggaca taacatcgct cagctatcgc
    12901 ttggatggtc atttcgccaa gtgctggaga atcgcttaag agaaactgga tcacttcatt
    12961 ttctgaagga gaaaaggaga tttctttgat tttttcacgt aataacatgt agataccttc
    13021 ttcttatttt tttacgtaaa ttataacttt tttgaaaatg tt
//
LOCUS       DAYYCW010000079        13043 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000079 DAYYCW010000000
VERSION     DAYYCW010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.081"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(252..866)
                     /locus_tag="ACHAYA_002085"
     CDS             complement(252..866)
                     /locus_tag="ACHAYA_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8605873.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1472..1849)
                     /locus_tag="ACHAYA_002086"
     CDS             complement(1472..1849)
                     /locus_tag="ACHAYA_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605874.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2149..3030)
                     /gene="galU"
                     /locus_tag="ACHAYA_002087"
     CDS             complement(2149..3030)
                     /gene="galU"
                     /locus_tag="ACHAYA_002087"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8605875.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3073..4092)
                     /locus_tag="ACHAYA_002088"
     CDS             complement(3073..4092)
                     /locus_tag="ACHAYA_002088"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8605876.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4725..5729)
                     /locus_tag="ACHAYA_002089"
     CDS             complement(4725..5729)
                     /locus_tag="ACHAYA_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8605877.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5742..6791)
                     /locus_tag="ACHAYA_002090"
     CDS             complement(5742..6791)
                     /locus_tag="ACHAYA_002090"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8605878.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6808..7629)
                     /locus_tag="ACHAYA_002091"
     CDS             complement(6808..7629)
                     /locus_tag="ACHAYA_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8605879.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7616..8395)
                     /locus_tag="ACHAYA_002092"
     CDS             complement(7616..8395)
                     /locus_tag="ACHAYA_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8605880.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8412..8882)
                     /locus_tag="ACHAYA_002093"
     CDS             complement(8412..8882)
                     /locus_tag="ACHAYA_002093"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8605881.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(8894..9313)
                     /locus_tag="ACHAYA_002094"
     CDS             complement(8894..9313)
                     /locus_tag="ACHAYA_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8605882.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9394..12171)
                     /locus_tag="ACHAYA_002095"
     CDS             complement(9394..12171)
                     /locus_tag="ACHAYA_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8605883.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            12362..12586
                     /locus_tag="ACHAYA_002096"
     CDS             12362..12586
                     /locus_tag="ACHAYA_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605884.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3819 a         2733 c         2118 g         4373 t
ORIGIN      
        1 tttccactac tttttatttt tctgacacta catattctta taactcattt ctcataactg
       61 tgaaacaact tataaaaata acaaacttta aacaaaacaa gcttaaaatc aaagatgatc
      121 catcttaaaa taactaaaac aaaaaaagta gtcgttgtta atcctcatta ataatcgttt
      181 tctttttttc aaacgctcca tttttatgtg gaacattatt tttttaagga actttgtcaa
      241 catgtggata actattttcc acttgtgtcc tggactagct gttttgctgt aaaatacaaa
      301 acttgctgca gcaaaaagat cgttgcaaga gaggctgttc cgcagatcgc tggatctaga
      361 tttggcaaag tcaagactgc atcatgtaaa ttggcgtggt tgtccaaaac caaatcagca
      421 aattcaaata atcgcaaacc actgctatgt tgcgatttga tacttcgtga caaatcaaat
      481 ccagtaacaa tgattaatgg atgaccattt tctttgatcc actgagctag ttcgatgatt
      541 gccgggtttc ttccttcatt cgaaagaaga agaaaaatgt cttctgattt gacagaaagc
      601 tgatcaataa ttgcctgtcc tgcaccttct agcgtttcat agacacctcc ggctggattt
      661 tcaatcaata tacttggtac aagatccggg gtttgatccc aaaattcaaa tgctacacca
      721 cttagtgtgc ggctagcata tatctgcaat ctaccacctt gattaatttg tttcgcgatc
      781 caagtgccag cttgaagcat cagcggctct tctatcctta atttctcttc caattggtga
      841 taatagtcaa aataacataa actcatttat ctctcccgcc tagctatact tgacatgaat
      901 agaaacagct gcctaatcta aggaagtaca taaaaaaaca gtagttagac agtaacacat
      961 gtggtctttg ctgtttcaaa cacaaaaaag gcattgaacg aatacgccca ataccttgac
     1021 atagcaaata aaacaacgtg cagaccatgg aaaatatcta cataggggta gaattttcac
     1081 cctagattag gtacaataaa ttaggtcgat gcactgcatt gactatggca aaatgatcgg
     1141 tcagggtgta tgtgtgtgac tggtcattcc ctgattctta gcgccaacta agggtcaggg
     1201 agcctttttt tattcagctg tttctatctg aatttattgt aagtaatctc ttatccaagt
     1261 gcaagcaaga aaatacaaaa aaacattggt atgatttata tatatataaa taacataaaa
     1321 aaaggaatgg cttatcccga tacaacaagc attcctttaa aaaagattct tttatataag
     1381 tgaaaaaagc ttttttccac caattaaata aatctctcat ctcaatagtt tatccattat
     1441 tttatctttt tttgcgtata cctactaaat attaccgtct tcttctcgtt cttctacttt
     1501 gttcgagaga atctgtacta gaaaaagtag ttgtatgtct accttttctg cctatccgag
     1561 actttctttt catatatggg atataaatga ttagatacag tacagcaaaa aataaagcaa
     1621 atacgcaagc ttcccaacta cctaacgcca attcaaccat caaggtagca accccaacgg
     1681 atgctgcata ccagataaaa atcagaaacg tttctttttt tctgaccatg cgaaacatga
     1741 taaatagact aataagcaat agtggcaaca caaatatcca attgcttcct tccatcagcc
     1801 acttgagtat atcaagcagc gactgtgcaa cagcgactaa ttttcccata gtgaacacac
     1861 accttttttt ataattaaaa aattcgcctt tttattctac tatgaatctt ctggcttggc
     1921 tttcatttct cttatgtaat cattttcttt tatacaactt aaaaaaaaat taaaccatta
     1981 gcataatatt ttctgaatct attcacgaaa gtctttttct atctttcgtt ttccacgact
     2041 caactgatct agagtcaaaa aactaaaacg acagttgcgc aaatgacgca caagctgccg
     2101 ctttaataac aaatagcctt tcattctcag caggccactt atttccagtt aactttctgt
     2161 tcttagcttt ttactcaact ggataatata tgcacttaat gaagtcccaa tttccgaatg
     2221 ttgaagtcca tattcaatat ttgtcttcat atatcctaac ttatcgccta cgtcgtatct
     2281 ttttccttta aactctctcg catagaccgc ctctgtctgg ttaagagtct gaagtgcatc
     2341 cgttagctgt atctcgtttc ctgctcctgg ttcttgagtt tctaatatct caaatatttt
     2401 aggagtcagt agataacgtc cgataatggc taaattactt ggtgcttttc ccatttcagg
     2461 tttttctaca aaccctttta ctctgcaacg acccttttcg ctatctgttt taggatcaat
     2521 aattccgtat ttagatgttt cattttctgg tacacgcatg acggcaacag cggaagcttg
     2581 tgtttcttca taatccatca tcaattgttg agtcaatggg atatcatctt tcatcaaatc
     2641 atcccccagc atgacgacaa acggttcatt tcctataaat gcttttgctt gaagcacggc
     2701 atcccccagt cctcttggat gagattgacg gatgaagtga agattgactt gagtcgtttc
     2761 ttcaactaaa gcgagtaatt ctgttttttc cttttcccgc aaattgtttt ccaattcgat
     2821 atttgcatca aagtgatctt caatgggtct tttttctttt cccgttacga tcaagatgtc
     2881 ttcgattccc gaatccaatg cttcttccac aataaactgg atcgttggtt tatccacgat
     2941 tggaagcatt tcttttgcca tggcttttgt tgccggcaaa aaacgtgttc ctagcccagc
     3001 ggcaggaatg actgcttttt tgaccttcac ttgctgttcc tcctaaacga cgattaacta
     3061 ttgatcatct atttaacgaa gagaaaattc attctccatt tttccgtcac gtaacatgat
     3121 ttcttttgct gcttttttga tatcttctcc attatacaaa acattataga ttgtttgagt
     3181 aattggcatc tcaacaccta gctgttgagc tagctctact gctgcttttg tcgttgccac
     3241 accctcaacg accattccca tgttttctag aacttcttcc agcttatgtc cctgtcctaa
     3301 caagttaccg gcacgccaat tgcgagaatg aacacttgta caagtaacga tcaagtcccc
     3361 cacaccactc aaaccgataa atgtcagagg attggcaccc atcgcgacac caagacggct
     3421 gatttctgcc aatcctcgag tcatgattgc tgcttttgca tcatctccga atcctaatcc
     3481 atggatagcg ccggcaccaa ttgcaatgat atttttcagt gctgcaccag tttctacgcc
     3541 aatcacatca ttatttgtat aaatcctgaa ataatcattc atgaacagtt tttgtacata
     3601 ggctgctgct ttttgatcaa tacttgcagc tgtaattgtt gtgatatcat gtactgccac
     3661 ttcctcagca tggctaggac cagataatac aacaattgcc tgtctttttt cttctggaat
     3721 ttcttcagcc aaaacttctg agattcgttt atgcgtatct tgttctagtc ctttacttgc
     3781 atgtataatg attggcttag tattcatttt accaataagt tcctgtgcga ctgaacgaat
     3841 cgcttttgtt ggaacaacaa ataaaatagc atctgcgtta tccactgctt ctgccaattc
     3901 tttatatccg ataatcgatt ctgggatcgt caaatcaggt aaaaaatgct gatttgtatg
     3961 atacttattg atttcatcaa tctgttcagg tacatttccc caaatacgga catcatgccc
     4021 gttttctgcc aatgtttgtg ctaaagcagt gccccaagat ccaggaccaa gaactgcgat
     4081 tttttgtttc atggatattc ctccaaataa ataattgata atttccatta tagtatacat
     4141 ttcttaaagt tgggactaat ttccattgtt tttaagtgtt tgtttacttc ttttatcgtt
     4201 tttacttgaa ataatcaatg atatgttaca atagtctaca ttttattttt acactcgtta
     4261 tgctttgctt gttcctaaga ggaagcagtt ttgacaatgt tactaccagc attactatta
     4321 ggaggttagg aaacttctcc tagtgagtat tgatttaaaa tacataagag acagattgaa
     4381 catttctaaa tctgtcaggt cctgcaacgg acagattgtc tctgttgacg gttgacatag
     4441 gaacttttgt tttagtcaac attctggata caaaacgcta tgtaaactgg cgcaactcgt
     4501 tgtacttgga cttttactac ggatccatgc aacaactaaa tagtactatt gcataacaaa
     4561 aaatggttgg cacccacatg tcccattaaa cagattataa gttgaactgt agataatgca
     4621 aaaaatgtgt tttataacgt aattgatgcc aatattttaa aaaaataaag ggagcagtta
     4681 tcgctgctct ctttattttt tatattcttt ttatccagtc tatcctaata atcaaactga
     4741 cgatagtagc ggcgtatctc taatggatgg cagttcaact gctccacatg agcatcgatg
     4801 cgttgtgtca ctacatgcat caataagaaa gacaaaacac gtccgcgata tttttcactg
     4861 ataccttcta ctggatagtc ttttgtgtct atcaatgtat aattgctgca aatttttggc
     4921 aaaagtttag ccacacgttc agataacact ctctgttcat cttcgcctaa aaataatgtg
     4981 actggggttg tttcttctac gatctctaac gtcccgtgaa gaaactcggc agcatggata
     5041 gatttcgatg gaagccagct ttgttcttcc cagtaacaca tggcatatga ataaacagct
     5101 ccccattgat tccctgctcc aataaaataa tgcatactat cacgtcgatg tttttctgca
     5161 aatgataagc caaatgcatc tgcttttttc tctgcttcca ctaaccctgt tggtaaataa
     5221 cgttcgagct gttcatagta ttctgaataa gcttcgaatt ctccattcaa atacatcaat
     5281 cgatctgctg ccataaaaaa tttgatttgt tctgttccag gagctggata agtgaccaca
     5341 aaatcgcata gctgagacaa cttactgttc gctttatcga taaatccaat cagtgtcgct
     5401 cctactttct tgatttgttc tactgctttc actacttctt gagttgtacc ggtaacagag
     5461 gacaaaataa cgatagaatc ttttgttaat cttttgtttc cagtagtgta atattctgca
     5521 gcatgctgta caaagacagg caagttgctt ttcccattca tataagtgac agcttgcatc
     5581 gaggaagcat acgttccacc gattcctaga taatagacag cgtcaaattt ttccgcataa
     5641 agtttatcga tgatttcttc tacttgcgga cgtaaagcta gtgcgccttt tattgctttg
     5701 atttgttctt cttcattgaa tttcagcatg atttccacct cttataattt acttttcaag
     5761 actgtatcaa agtcttcaaa aatctttttg ttcaagtcaa tacctttagc tactgcggtt
     5821 ttataagcga atagttgaaa cggaacgaca agcgctaata cagacagata ttcatcacat
     5881 tctattgcta atccaagtgt tttctcgttt cttgcttttt ttgtggtaat ggtgagagtc
     5941 tctccaacgt accgactcat atatctttgc aacttttgcg aacgttcctt atttacactt
     6001 tccccttcaa taaagaatag caaatgcgaa gcatctgcct ccaggtatgg gccgtgcata
     6061 taagcttcta actcaaatcc ttgtgacgga acgcggattg tttcagtcaa tttcgtctct
     6121 gcttctttag ctgttcccca gtttggacca tatccaatgg tgacgaaacg ctgtgctaaa
     6181 cgaaataaac tttgatatcc atcaaaaaat tcttccgttt tttgaataat cgctggtaaa
     6241 tggttgatta ttttgcgcaa ctgttgcatg tagtcttgtt ctattttttt gctgatttta
     6301 tttttactta ttccaattcc tatccctaag agtagtaact gtaaaacagt cgcagaatag
     6361 ccttttgtaa cgaagcctac tgattctacc ccggcaccta aatcaataat ttgtgcagct
     6421 tctaaagcaa gtggactttg cacgtcattt gtgatagcaa caacaggtaa cccttgtttt
     6481 tgtatcattt ttactgcttc aacagtagaa gcacttttcc cgctttgtga cacagcaatc
     6541 acagtatcga ttgaaggaga aagttttcca taattgaaaa aataaaaagg ttcttgaata
     6601 tcgacagtaa gatcaccata agattccaat gctggcttcg ctgctaaaca agcgttataa
     6661 gatgaacccg ttgctaagat cagtaaattc actgtttttt tcatatgttc tcttgtcgaa
     6721 aaatcattct ttttcaagat cgtacttagt acttcctttt cttcatagat ataatcttgc
     6781 atcgtaaaca ttttctctgc tcctttttta aaaaatccct atccacgaac caaaaattcc
     6841 aaagaaagct agtccgatca agatcgttaa tggtttcact tctttcttca atagataaaa
     6901 cactatgcca aatgctccaa gacataagat atttggaacg atatcatcaa aaatactttg
     6961 aacagtcaca gcattttctc ctgaaccgat ttttaaagga atattgatgg tgatcatcgt
     7021 ggcagtcatt gctccaacga ccatcagtcc aataatagac gcgcctaatg tcaaactttc
     7081 catcataccg tttgcctgga tctttttcaa aaaacccgtt cctaagcgat atccccaacc
     7141 tgttgctaga tagcgaaaca gaatgtgcgg aatattgaaa atcaaaatga acaagatagg
     7201 gcctaaaata tttccttgca tggctaaaga ggtacccaca cctgtggcaa tcaatcgcaa
     7261 agttccccaa aagaaagaat ctcctaaccc tgcaagaggt cccatcaaag atgttttgat
     7321 actatcgatc gcagtcacat caaattcagg atcatcctta ttttcttcct ccattgcagc
     7381 cgtgattcct aaaatcagtg taacgatatg tggggtcgtg ttaaaaaact ctagatgccg
     7441 tttcaaagct gctgccattt cttctttcga ctgatataac tttttcaaaa tcggaatcat
     7501 tgcataaaca taagccatat tcatctgacg ttcataattc caagaaaact ccatttgaaa
     7561 agaacgccaa aatacgtgat ttagctcttt tttcgttatt ttgctgtttt tgatctcaga
     7621 attcttcatc ttcttctacc tcccctgcag ttgtttgaag tggtagttga cggacattca
     7681 taatattgac taccacaact gctaaaatcg caccaaaaat agctactccg gtcacaggaa
     7741 tctctaagta agaagctata gcaaaaccta agaaaaaata tggtgcaact tgtttgttga
     7801 tcagcaaacg agcaagcatc gcaaaaccaa gagctggaat caaccctgta gctactgaca
     7861 accccttttg aacgaagtca ggaatcatat ttaatagatt tccgatcgta ttgcttccca
     7921 ccatataaga aaccattacc acaacaccaa gcatcaagga aagaccgata ccggcaagca
     7981 agtgcatatg ctcgactcct ttataatttc cttcttctgc ataattgtct gctttatggt
     8041 tcataattgg aatcaaaagc cctaagtaaa tatttttcaa tatcaatgtc aacgtagcaa
     8101 tcggcaaacc tagtaaaagc gcggtttctg taccggctcc agctgtaata gcaaaagccg
     8161 tccctaaaat cccaccggtc acaacatcag gtggaatcga agcacctact gagaacgatc
     8221 cgataaatgc taactctaaa gttgcgccca taatgatccc agtttttaca tcacctaaca
     8281 cgattcctgt aaacagcccc gtcacaatcg gacgagaaag caacgatgtc cctagtgcat
     8341 attctgattg tgcgacaaat gcaacaattc ctaataagac tgcttgtaca atcattgagg
     8401 ttcttccttt cttacaaaac ttctcttaca tccacttttt tatctgctgg tacttgacgg
     8461 atttctactt ctatttgttt gtcaattaat cgtttcaagc gttcctcctc ttctggtaaa
     8521 atatttaccg cttttccgat gctgcgagta ccaggttttg ccttgatccc tcctaagttg
     8581 atggttttga tttctggata cgcttctgcc agcttatatg catcctcgat cgattccact
     8641 acgatgaata acttatattt atctgtcacc ccgcttttca acgcggcaat cgagtcatca
     8701 attgatttga tcactagttt gactccttgt ggcttggcta atttgatcgt tgtctttcgt
     8761 atctcattcg taggtacatc atcattagca atcaaaatac agtctgcgcc tattgtttgt
     8821 gtccatgaaa aagctacttg tccgtgtaat aaccgatggt cgactcttgt caatacaatc
     8881 atttttcttc ctcctaaaat tcgtcttctt ctattttctg attgagccgc tcgttacaaa
     8941 actggatcat tttttttgaa tcagttagca cctgctggat caaaacagtt gtctctactt
     9001 cagcgtccaa ccgagatact agctcaatca atagtggcaa attcaatccg gcaacgagat
     9061 agacatgatc ccgattgata tattgtaaga attcgttgtt gacacttccg ccaaaaagat
     9121 ccgtcagaac aatgatttct ttatctttgt acctcataaa aagctgcctt actgtttccg
     9181 ctaaatcaaa attctcatca gtatagcaat cgaatacagt aatagaatga tttttcccat
     9241 agatcagctc taatgaatct aatatccctg aagcaaattt tccatgggaa gctaagataa
     9301 tttctctatc cactttttca tctcctcttt tcacttacac atactaaaaa agcaagttgc
     9361 atgccaaaat tagtggcaac aacttgctta tgttcaaaat tcttcttcta tcgagagaaa
     9421 ctctgtatct tcactcaaaa tatcatagat ataggcaatc tcagaattgg ggatcatgac
     9481 actataatgc tcttcaatga cactaaacgc agatttaata gcagataacc cttctttttg
     9541 acactgcttc catttctcta atccttgata ggtttcgatt ggcatttgtc ggatcaatcg
     9601 ttcaatgaga cagctcacat ggacatacaa cgctaatttt ttgggattgc tgatgcttcg
     9661 attcaactgc acctctaatt ctctcataaa atgatcgatt tctcccatca ccttctctgt
     9721 atctagaata gtcacggaat ccagtacttt ttccaatgaa aaattacgaa tgatgcgctg
     9781 attgaatatt tccttctctg aagcattcat agtagtgtcc aaccattcag ctagtacaga
     9841 catcttttcc cccgagatga ggtcttctaa tgaaagatag tcgatattgg tgatatcagg
     9901 atctgtggtg ccaatcattc ctacgacttc gtatatggaa aataatgggt cactgttttt
     9961 cttatctttt aattgctgat attcatatgg gatgattttc aactgacaag ccgtaggtaa
    10021 gcttttttct agcagttcac ttaagtggac cgccgtaccg attccagtga agcaagtagt
    10081 aatgagtgct ttgggtttgt tttcctctgg atagagcagt cgccattctg gctggatctg
    10141 tcgaacagtc tcttctgcta tctcttctaa tgatacattt ttttgcaggc attcccctac
    10201 tgtgattgct aaaggtgtcg tcacattatt caaaatcaaa agcggtacgg ataactgttt
    10261 tttaaaaaac tgatggattt ctttcagaga tcccatgtct actaatatga tcaatccatt
    10321 tgaaacatcg ctgttttcgc aataatcaag gatttctgaa gcaatctgct gtggtgtgac
    10381 atctagtggc atatcaaatg attcaaatag atcctcttgt aataaacgat ttactacatt
    10441 tgccatactg ctagcagttg cataaccgtg agcagcaatc atcgcttttg gcagtcgttt
    10501 ttcttttgac catttcgctt tttttagata gatcgtcagt aaaatccgat ccatacttgt
    10561 gtcttcaata tctaatttga tcttacttaa ttccagaatt cgttggacat agtgataact
    10621 agatggataa gactgagaaa cttgttgttc taattctttg attagttgga taatatcatg
    10681 ttcttccggt aaccatttga tagtgcttcg ctgatagaga tagtaactca aggcatagac
    10741 actgtttcca tcgaatttga tttgataggc agtttccatt tgtcgaaagg tctctcggat
    10801 agattgtgtc caataaacca gcagctcgtg tttttcttgt ctactggtct caaacagcaa
    10861 aaaatcaaat aattgaatca cttcttgctt taattttcct tcgcaagaat atagcagaca
    10921 gttatttttc ttgaaatcaa ttaggatttg ctcaaaactt ttaacgattc gctgctgttc
    10981 gggctgttgc ttctccatta attgctctaa ggtctggtct tgcgtaatat agattccttg
    11041 ctgtgctgta tttttagtaa taggctgcgc aagtagttct ttttgcaaat gatggatcgt
    11101 gacatggatt tcaggctgtt cacgctgttc agcatatgct tttgctgctg aaactttgac
    11161 gatatttttc agctctccta tgtttccgga taatttctga cttgttaaaa gtgacaaagc
    11221 tggtccactg actgacaaag ggctccccat ttttctttgt tcttcgagaa aaaaagagta
    11281 aatcagttct agccgttcat tacgactacg ctgattaagt gcaggtagtt cgattcggat
    11341 aggtattctt cgaatgaaag ttgttaagaa atgactagta atctcttcag tcgtagcaaa
    11401 aaagagacgt gttttgatcc ggcgtgacaa attaggttct cccatacgat aaatcacacc
    11461 ttgatcaaga taggtgaaca gtttttcctg accttctggt gttaagcgat gtacctcgtc
    11521 taaaaacaag atcccaccat ctgctgcttc aaaagcccct tttttatttt cttctgcacc
    11581 agtgtacgct cccttaacat gtccaaacaa attgctagtc aaaagttcag gattatctgc
    11641 atattgtgca caattaaaag tgatgaatgg tgcagaatct tctagcagat catgaagcag
    11701 acaatactga tagaccaaat taactaaata acttttacct gtgccacttt cgcctgtaat
    11761 caaaagtggt aatccgccat ctggatagta aagagctgtt ttgatttgtt cgatactttc
    11821 tttcaaactg gcgtcgtatc cgatcataaa agagaataag tcttttggtt gcgcgaaaaa
    11881 aggctgttcc tctttgagat catcgataga aaaataaaaa ttcgtgcgta acttaaaaaa
    11941 ttgtttttca aaagcttctt tgtggaaata aatcactggt cgagaattga ttttgactag
    12001 tagttgctca tcatttaact ggttcaaata atgactgact gtgtttcttt ttaccccaaa
    12061 atgatcagct aacctttgtg ctgtaaagac ctcgttcata tgttctaaat cgaaaaaggc
    12121 tgtctggttc tctaagtaac ataaaatttc cttcttcaac aacttattca cccctcctcc
    12181 atcatagaaa acggattcat tttgtcaata aaatctttaa taaagaattg aaaaatatat
    12241 cttattatag acagcagcct acttatccct tataattttt attctaaaaa acgtacagtt
    12301 ccttccagtt tttatactgg aatttcaaca tatgaaagaa taaggaggct atgactattc
    12361 aatgaaatcg gataaatata aaaacaggaa acatacttcc tggaaaactt ttcgagaaat
    12421 catcggatct tatctcgctg tagttgtatt tttgcctttg ctttatatgg ctatgacaga
    12481 acaaactcgt tttattggcg tagatacgca atatatagcc gaaacaggat ttttattcat
    12541 ctttatcttg atttttgcgt tctataaatc cttccataca aaataagatt cacagatttc
    12601 aactttcctg tctgttttta gctattacaa atccttatat gtggtataac aacaatttat
    12661 tggcataatg aggaaaaacg agttatgatg ctttcagaga gtaaagaggt actgttgtca
    12721 ccatcctttt tattgattac gtacaccact cttagttttt tcccattgat tacgaaaaca
    12781 aggtagaaac attgatgttc ctaccttgtt ttcttttttt attttcagat caaaatctca
    12841 agtggctttt gacttgatgt cgggaggttg tcttcgttcc atcaaacctg atagaatctg
    12901 cgccaaacca tagttttctc cactattatt tattcctcta agatagtacc cacaataaac
    12961 gaataaatat ttttctcatt ttcatactcc ttctttgaaa atcaattaga tttctctcaa
    13021 cccacttttt tacaaaaatt acg
//
LOCUS       DAYYCW010000080        12931 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000080 DAYYCW010000000
VERSION     DAYYCW010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.082"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(548..910)
                     /locus_tag="ACHAYA_002097"
     CDS             complement(548..910)
                     /locus_tag="ACHAYA_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605885.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(984..1364)
                     /locus_tag="ACHAYA_002098"
     CDS             complement(984..1364)
                     /locus_tag="ACHAYA_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM8605886.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1446..2621
                     /locus_tag="ACHAYA_002099"
     CDS             1446..2621
                     /locus_tag="ACHAYA_002099"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM8605887.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2698..3282)
                     /locus_tag="ACHAYA_002100"
     CDS             complement(2698..3282)
                     /locus_tag="ACHAYA_002100"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8605888.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3483..4481
                     /locus_tag="ACHAYA_002101"
     CDS             3483..4481
                     /locus_tag="ACHAYA_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8605889.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4593..5102
                     /locus_tag="ACHAYA_002102"
     CDS             4593..5102
                     /locus_tag="ACHAYA_002102"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM8605890.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(5132..5791)
                     /locus_tag="ACHAYA_002103"
     CDS             complement(5132..5791)
                     /locus_tag="ACHAYA_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM8605891.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5793..6557)
                     /locus_tag="ACHAYA_002104"
     CDS             complement(5793..6557)
                     /locus_tag="ACHAYA_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM8605892.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6565..7242)
                     /locus_tag="ACHAYA_002105"
     CDS             complement(6565..7242)
                     /locus_tag="ACHAYA_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM8605893.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7279..8664)
                     /locus_tag="ACHAYA_002106"
     CDS             complement(7279..8664)
                     /locus_tag="ACHAYA_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM8605894.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8874..8958)
                     /gene="ffs"
                     /locus_tag="ACHAYA_002107"
     ncRNA           complement(8874..8958)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHAYA_002107"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8981..9496)
                     /gene="tadA"
                     /locus_tag="ACHAYA_002108"
     CDS             complement(8981..9496)
                     /gene="tadA"
                     /locus_tag="ACHAYA_002108"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM8605895.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9602..10459
                     /locus_tag="ACHAYA_002109"
     CDS             9602..10459
                     /locus_tag="ACHAYA_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8605896.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10540..10875)
                     /locus_tag="ACHAYA_002110"
     CDS             complement(10540..10875)
                     /locus_tag="ACHAYA_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605897.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10942..12312)
                     /locus_tag="ACHAYA_002111"
     CDS             complement(10942..12312)
                     /locus_tag="ACHAYA_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM8605898.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12390..12926)
                     /locus_tag="ACHAYA_002112"
     CDS             complement(12390..12926)
                     /locus_tag="ACHAYA_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8605899.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3834 a         2612 c         2158 g         4327 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagcgattgt caactctttt ttaaaagttt ttttcgattt cgtttttcga aatctttcag
      121 caactcattt atcataactt atcgtttgtt atttgtcaag aacttttttg aagttttttt
      181 cgatagaatc aagcttcgca agtggttctt ttttaacgac tcagttatct tatcatgtcg
      241 ttagtaacat gtcaataact attttttaaa ttttttagaa atgtgatata acatccactt
      301 cagaatcaac gctgttaaat ttatcatgta tttcttcctt ggtcaactat aaattaagca
      361 ttactttgtg aaatgatttt tcgatcaatt attattattc gttatacaac cgttttcaac
      421 atattttgaa aataaaatgc actaaaaaac tatctttagc agaacatttc attgtatttt
      481 ttggaccaaa caataaaaaa agaagtttga aaaaaattca aacttctttg ttcgcttaat
      541 ctgtatttta gaagaaaggc actccctcta cttcgtaagt tccaagaaca gagcgcattt
      601 gacctggttc ttcccaaata tatccttcgc ctaattcttc tctttgattc ttttgttcag
      661 tttcaaagac aaacaacgga acattttcct ctttaatatg tgtatttgtc aattccatca
      721 aactaatctt gctaacatcg atatgaacta tttcttttaa gaaattaagt atattcgcta
      781 aaccagtctt tccttcagaa agttccgcta acgcaaattc caacttgtca tcaattgaat
      841 ggaccaaaaa ttttttagta ccatcctcca aatgcaacat aattgtacca gcaactcttt
      901 tgcttgccaa aatttccacc tcgatcaact tgttctatgg cttacataaa aattctagca
      961 cattttcaga aaaaatacac tttttatttc ttattttctt tccgttctag ttcagataat
     1021 gcttcttcta tccaaatagg taactgctcc gcaatagtgg taaatccgat cttacgattt
     1081 ttattcgtga aatacctttt tctgcgatat ggaacagctg gatttgtttt ttctaacgtt
     1141 cttaaagata aactgatttt tttggaatat tcatcaatgt cgatgatctg aactgttact
     1201 ggctgtccga cttttaacaa tgaatggata tttttcgtat aaccggattg tacttctgaa
     1261 acatgaataa gtccttgcgt ctcgttatca agagaaacga atgcgccata aggctgtatc
     1321 cctgtcactt ttccttcaat tatttgtcca attttataag tcatctactt ccctctcttc
     1381 gcatttatca ttatactgta aatagctcaa aaaaagaagc gacgaaaatg actggaggtc
     1441 ttaagatgat cgaatttgat aaacagattt caagaaaaga tacagaaagt gtaaaatggg
     1501 acgcaattgc tgagacatat caaatggatg acttacttcc tttgtgggta gcagatatgg
     1561 acttcttgtc tcccggtggg gtagctaaag cgtttagtga atacatcaaa catggaatct
     1621 ttggttatgc cactctgtct gataaactat atcaggctat cattcattgg gaacgccaac
     1681 accatgctgt ggagttgacg aaagaaaata tcgtttttac aagtggtgtg ttaaccagtt
     1741 tagctgcagc agtacaaact tttacaaaac caggcgattc tattttgatt catgacccag
     1801 tttatcctcc attttcctct attatagaaa caaatcaacg tcacatcgtt cgaagctctt
     1861 tgctagaaaa aaataatcat ttcatcatgg acttgtctga catggagaaa aaaatcgaag
     1921 aaaacaatgt aaaagcaatg attttatgca atcctcacaa tcctggaggg cgtgtttgga
     1981 gcaaagaaga attgaaacag ttaagcgaat tgtgtttaaa acatcaagtg attctgttta
     2041 gtgacgagat ccatcaagat ttaactttat tcgatcatac atttacttcc atgctaacga
     2101 ttgatcctaa tttagatgat ctactagttg cttttacttc cgcaaccaag actttcaatc
     2161 tagctgcaat caaaaattca atggtattta tcaaaaatcc agagatgaaa gctgattttg
     2221 aaaaacattt agtgatgaac caacagcacg aaatcaacac ttttggactg attggaaccc
     2281 aagcggctta tgagacgggt ggagaatggt taagccaact gattccttat ctagaaaaaa
     2341 atgttcaaac agttaatcag ttttttgaac agtatcttcc gaaagtgaga ataatgaacc
     2401 ctgaaggaac ttacttaatg tggttagatt ttaccgccta tatttcagac gaccaagaat
     2461 tggagaacac tcttgttcgg aaaggaaaag tcgtattaaa tcctggcatc acgtttggtc
     2521 cttccggtca tgggcatatg cgcttgaata ttgcttgtcc agaagaaact ttactggaag
     2581 gacttaatcg aatcaagtat gctttagaga caatcgatta aatcatttat tcaaaataaa
     2641 aggacgagac cttactaggt ttcgtccttt ttcataatta ctccgtacca taccaaacta
     2701 ttctgaaatt tcaattgttt caatcactac atcatgtaca ggtcggtctt gtggaccacg
     2761 ttgtacattt gcgatttcat ctactgtgtc catcccttct agtacatgtc caaagactgt
     2821 atgtcggaaa tctagccaag gagttccgcc acctttatac gcttcaatga tttctgatgg
     2881 gaatccagca ccttctagtt gtccaagcat attagctggc acatttgtgt tatttacaat
     2941 aaagaattgg cttccgtttg tgtttggacc tgaatttgcc atagataaag ctccgcgaag
     3001 gttgaacagt tcacgactga attcatcttc aaaactttcg ccataaatac tttcgccacc
     3061 cattcctgtt ccagttggat cgccgccttg gatcatgaaa tcaggaataa cgcggtgaaa
     3121 aatcacgcca tcataataac cttttttgga aagttcaatg aaattttgaa ccgtcttcgg
     3181 tgctagttca gggaataatt gaacagtaat gtcaccacgg tttgttttga taacagcttt
     3241 tggtcctttt tcacttttta aatctagttg tgggaatgcc atattgtttc ctcctaatat
     3301 ttgcatactt cataataata ataacataat acctaacctc tcgtaaaaat actagatttg
     3361 ttttctttta tttttcatca ctagatacat tctataactt accgtttcga tttcgaattc
     3421 aaacattttt gagaagcatt ctcaattaaa ttctgatata gtgtgaaaag ggaggaattt
     3481 ttatggaaat ctatgacatt acaatcatcg gtgcaggtcc tgtagggatg tttgccgctt
     3541 tttacgcagg aatgcgccaa gctaaaacaa aaatcattga cagtctacct caacttggtg
     3601 gtcaattagc aactttgtat cccgaaaaat atatttacga tattcctggg tatccagcaa
     3661 taaaagcaag tgaattaatc gatcaattag aaaaacaatt aactactttt aaccatacat
     3721 tccatttaaa agaagaagtt ctctcgctta ctcgtgaaga cgaagttatc gagattacta
     3781 caaataaagg catccattat tccaaagcag tcattttagc tttaggaaat ggttcattcc
     3841 agccaagaaa attgaattta gataatgctg aatcatttga aaaccacgga ttggattatt
     3901 tcgtgaatga tttgatgaag tatgctggaa aaaaagtcgc tattgctgga ggtggtgatt
     3961 ctgctatcga ttggacgttg atgttagagc ctattgcaag cgaagtttac ttgattcatc
     4021 gtcgcccgga atttcgtgcg cacgaacata gtgtatcacg cttgaaatct tcatctgtca
     4081 acttgcttac tccctatctc atcgatggat tatcaggcaa taatggggaa ctgacagata
     4141 tccggttaaa aaaagtgaaa agtgacgaga cgattgattt gatgattgat tctctgatcg
     4201 tcaattatgg attctcttct aatttagaac atctttcttc ttggggactt gatagtacca
     4261 gaaatgccat tactgtaaaa tcagatatgt ctacttctat tccaggcgtt tatgcagctg
     4321 gtgacatctg tacctatgaa ggaaaagtga aactgattgc tactgggctc ggtgaagcac
     4381 ctactgcagt gaacaacgct ttacactata ttaatccaaa ggaacgaact cagcctggtc
     4441 acagcacaag cttatacgat aaaaatcttc gtcctagttg attttttata tctctttgca
     4501 tttcaagtta aacccgaaag cagtttcttg catttttttc atgttccgtt aacatgaaat
     4561 agagaaagaa aaaataaagg gggagcaata atatggtcgt caaaactgag actttagaag
     4621 aaaaggcaag agaactattg aaggaacgtg gcgtaactgt agaagatatc gcacaacttg
     4681 tcatgttttt acaaaaagac tatatttctg atttgacttt ggcggattgt actgaaagtg
     4741 tcaacagcgt gctaacaaag cgtgaagtac ataacgccat tatcactgga atccaattag
     4801 atatattagc agaaaagaaa aaattgatga gtccgttgca acatattatc gaagatgacg
     4861 aaggccttta cggtatcgat gagatcctag ccttgtcaat cgtgaatgtt tacggtacga
     4921 tcggctttac gaattatggt tatatcgaca aggtgaaacc tggtatttta aaagaactaa
     4981 acagccacga tggcgttcat gtccatacct tcttagatga tatcgtcgga gcgatcgccg
     5041 ctgctgctgc cagtcgatta gcacacaaag aaccagaaaa aagaagtcgc cttactcaat
     5101 aattcaacaa aaaaagaaag aaaacgtcta tttagacgag ttctttcttt tttgtttgtt
     5161 tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat aatacaaaaa
     5221 ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt ggatcagtaa
     5281 cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg ataaaaaagc
     5341 gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg ccgatttgaa
     5401 acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata aacaaaatac
     5461 tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt ttcacttcat
     5521 aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg atggttttga
     5581 agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc tcttgatcta
     5641 aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt ggtcgaaagt
     5701 tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac aatccagcat
     5761 attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc cagtgattgt
     5821 ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt aaaacctgat
     5881 aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt ttcatagtta
     5941 tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc tttatccata
     6001 atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt ctctacaaaa
     6061 gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt cttatctggg
     6121 ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac tactttttca
     6181 taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc ttcccatgta
     6241 aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata gacttttttc
     6301 ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta gactgtctct
     6361 ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc tggtgactta
     6421 gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc ttccacaaat
     6481 cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa atcaattaaa
     6541 tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa atgctgtttt
     6601 tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg tttttgctga
     6661 ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg gtcctttttt
     6721 ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag ttgtcttttc
     6781 ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc aatattcgta
     6841 aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat tatccgcttg
     6901 gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat aatcatatcg
     6961 ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt cacgataatc
     7021 ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta acagtgtcac
     7081 tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca aagcagctgt
     7141 cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag caatttctgt
     7201 tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac cacctatctt
     7261 ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat ctcctaccac
     7321 acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa tcgttggaaa
     7381 aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat tcagatcgat
     7441 tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc cattatagac
     7501 catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt tacgtaaaaa
     7561 gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac ccaatgtgac
     7621 atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat tgacccgccc
     7681 ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg tctttcctcg
     7741 ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga tatctagact
     7801 cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc cacgttctag
     7861 acaactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg tagggatggc
     7921 tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga cttcaccgac
     7981 atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt gaccttctct
     8041 gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt cttttgcaat
     8101 ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca cttgtgaacg
     8161 taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc agccattcgg
     8221 attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg tctggtgttt
     8281 cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat ttttatcgaa
     8341 taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca actcttcttt
     8401 ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa cttcattcat
     8461 cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac gatccacaaa
     8521 atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt ctcgtttacg
     8581 ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca gatcattcgt
     8641 atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt atcataccat
     8701 aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg ccttgaagca
     8761 aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga acattcattt
     8821 ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt tcattataat
     8881 cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac gcttcacaag
     8941 cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca ttcgttgacc
     9001 agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca gtcaaaatca
     9061 tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta aaccgctcgt
     9121 cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac gcgccgaaat
     9181 aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa ggctctaagg
     9241 taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag gcttccttga
     9301 ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg ttatatcctc
     9361 ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca tgaagcattt
     9421 ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct actgcatcaa
     9481 gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta ctttagcata
     9541 tgataaaaag gctgtaacta actttttttc tatggtaaaa taaatacaag aggtgaacaa
     9601 gatgaaaatg gataaattac ttgctcttta cccacaagca cagaaaaaaa actctccagc
     9661 atcagaagcg gatatccttt cttttgcaat tgatgatgct tttatctgga tcaaacaaga
     9721 ttcattatct caacaagaaa ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca
     9781 aaaaaaacac ccatggtatc actatctttt taaagatgct tcttgtgttc aagaaaaaag
     9841 ctttcgtttg atccagcttc acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca
     9901 agatactatt ttggaattat ttcgctattc tgctgatttc tttttttata cagacacaga
     9961 tgcgctctta gtcgaacaaa taaaccgtga ttatcttgat gctactgaaa tcaacggtat
    10021 ttttctatct ttagatgctg attttgatac gacaacgtct gtattcgttg gttctttcca
    10081 ttcacctggc accgacctcg tccctttgtt tgctgaagaa agaaaaattt ttttaacaga
    10141 gcaaaacaat ctttatactc acagccgtac tttttctatg caagatgtgg ctttacatta
    10201 ctatacaaaa gatactgtaa aagatagtaa gttgatcaaa agttacatag aactgatcaa
    10261 acaaaatgaa atggaagtga tcattcaaga attatggaat gaattaggca atgtcagctc
    10321 tgctgcaaaa acactgtttc ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca
    10381 agaacagtct ggtttcaatc taaaaaaagc caacgattta ctgttttgct atcttttatt
    10441 gcttcatgaa cataattaaa gatagattaa aggggttgtg acaataattt tgtcacaacc
    10501 ccttatttcg aatgaatagt gttcaaaagc tagtttctct tacatcattt tttcaacaaa
    10561 atgcattagc cggtctttta catcttcggt tgaatctttg attgaatcac cataaacaga
    10621 gattttcttt tttcctgatt tgatttttgc taacattccc atcaaagaat ccaagtcact
    10681 atctgaaaga ccatctacga tttctagtaa tttttcattt ccatcaaatt tgtcatagac
    10741 gaattttttt actttcgtcc gagtagtcat atgatggatc ttttccatta cttttcccga
    10801 tacaacgact gctgtcgcta cactggcagc ggctgctacg cttaatccga ttgttacttt
    10861 tgttgatgtt ttcataagta tcagctcctt tttatctatc tccatcatag catagacaaa
    10921 aaaataaacg aactaatcca ctcagtgaaa tctcaatcct ttagggaaaa agttctttac
    10981 tgttcctttt acttgtttcc caaatccgac aggtatctta tctaatacta gcaaaaccca
    11041 tccttgattc ccatcacgtt gaaatgtttc tcctgctgtg taagattgcc attccttttg
    11101 agtgatcggc aagcaaggta tattctctat cttttttgta gcaagcgcta atgcatagct
    11161 aggttcaaat cgattctttt tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa
    11221 accatcgaga gaaggggcta actcaggaac ttcccaaaga tggtcgttga acactaaaag
    11281 tcttccagta gcttcataat gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc
    11341 ttttgtcatt tgcactttac ttttttttcg tgtcttcttt tctttatgca tctgattttg
    11401 tccatgaaaa gtcagctttg caacaaaatg tccttcaccc tgatctttat gaggccatat
    11461 acgtattgtt ttttccaaac cagctacgct tccccattca gaacgtcctg aactaacgga
    11521 ttgagtcaaa ggaatctcct caatcgttac tggataattt tctacaagcc agctaatgat
    11581 ctcttcattt tcttctggtg caaacgtaca agtcgaataa atcagttgtc ctttattttt
    11641 tagcattttt attgctgaag acaaaatctc ttgctgacgt ttttgacagt atagcggaga
    11701 ttcttctgtc cactctttta ttgcatttgg atcttttcgg aacattcctt ctcctgaaca
    11761 aggagcatca accacgatcc gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg
    11821 tgcatgattt gtcactattg cgttcgaaac tccccatcgt tctatatttt ctgataaaat
    11881 cttcgcacgt ttcggaaaga tctcattcgt cactaataac cctttcccct tcatttgagc
    11941 agctaattgg gttgatttac caccgggagc cgcacacaaa tccaaaactt tttcaccggg
    12001 ttttgctgca gcagctgttc caacgatcat tgcacttggt tcttggctat attcataccc
    12061 agcttgatgg aggaaagact ttccattgac agtgccaaga aatccctcat tgctgtaggg
    12121 agcgggttgt aattcttcgc tatggtaagt ttggaccatg tccaatcctg ccggttttag
    12181 tggattccac cgaaatcctt ttttcacgct tccttgttca agcgcagaaa aaaaatcgga
    12241 tgcttcttcg cctaaaagaa gacgatattt tttgataaat tgctgtggta acgttgtcgc
    12301 ttcctccttc attccaatgt ttcgttagac attttacaat cttttttacg aaaaataaag
    12361 gtggctgatc tactttttca tgttgctgcc taaggtgact gttcctttaa gatattttcc
    12421 aagtattcat ccgtcacctc gtttttatcc caaacagcgt tcatcatttc aagaaaatct
    12481 tgagaagaat catctgagcg caaacttccc aatgcattaa gccataccaa ttcaagcgac
    12541 ttttcttctt tgatcttcgt gtgacctttg acatatgaga aaaatggccg cttaaatagc
    12601 gaatccatgt ctaaatcaga ttttgcaatg atttcacgaa tactttcatg gatagaaaaa
    12661 aaatctttca ataagtagac aatgcttaaa acatcatcgg aactgcattc agctaagtag
    12721 ttttcaaaat aaacagtgat ttcttctaat atcttctctt ttaacccgct gattgatttg
    12781 aaatgtgtat aaatcgggta tgtagaacaa ccgcagaact ctgctaactc tcttgcagta
    12841 attgacttca ttccatattc ttccacgtat accatacttt tgaacagcaa atattcttta
    12901 ctataatttg ttactctccc catcattctt a
//
LOCUS       DAYYCW010000081        12925 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000081 DAYYCW010000000
VERSION     DAYYCW010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.083"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1557)
                     /locus_tag="ACHAYA_002113"
     CDS             complement(52..1557)
                     /locus_tag="ACHAYA_002113"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM8605900.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1649..2239)
                     /locus_tag="ACHAYA_002114"
     CDS             complement(1649..2239)
                     /locus_tag="ACHAYA_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8605901.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2418..4064
                     /locus_tag="ACHAYA_002115"
     CDS             2418..4064
                     /locus_tag="ACHAYA_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8605902.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4113..4832
                     /locus_tag="ACHAYA_002116"
     CDS             4113..4832
                     /locus_tag="ACHAYA_002116"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8605903.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5311..6258
                     /locus_tag="ACHAYA_002117"
     CDS             5311..6258
                     /locus_tag="ACHAYA_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8605904.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            6270..7190
                     /locus_tag="ACHAYA_002118"
     CDS             6270..7190
                     /locus_tag="ACHAYA_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8605905.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            7231..8688
                     /locus_tag="ACHAYA_002119"
     CDS             7231..8688
                     /locus_tag="ACHAYA_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8605906.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            9033..9647
                     /locus_tag="ACHAYA_002120"
     CDS             9033..9647
                     /locus_tag="ACHAYA_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8605907.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            9640..11364
                     /locus_tag="ACHAYA_002121"
     CDS             9640..11364
                     /locus_tag="ACHAYA_002121"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8605908.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            11361..12812
                     /locus_tag="ACHAYA_002122"
     CDS             11361..12812
                     /locus_tag="ACHAYA_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8605909.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         4190 a         2237 c         2591 g         3907 t
ORIGIN      
        1 cacagtccct ttttttctta gaaatgggaa accactagta tataaatacc attattcttc
       61 aatcaaccgc ttcacgattg cataatcccc ttcataaggt tcatctacac cattgatttt
      121 ttgataggga tccacgcctt ttcccgcaat aacgactgaa tcttgcggag cgctttctgc
      181 taaagcgcgt cgaatcgctt caggacgatc catttcataa tgaacgatca aatctggatt
      241 agtaattgct tcattgattt cttccgcgat ttttttcgga tcttcaaatg ctgggtcatc
      301 tgccgtcaaa aaggcgatgt cagctgtttc tgatagcact tttccaaaat catgtcgacg
      361 ggagattgct ttgtttcctg ggctgcctaa tacaacaatt acacggccat ctggatgttc
      421 atttttggca aattccagca atgttttcaa gcttagataa ttatgtgcat agtccacata
      481 gacatgcgct ccattggatt ggatcaattg atccattcgg cctggtacac gagcttcttt
      541 taatccgtct tgtgcatctt gactggaggc acctgttagt gctgcggcaa tgatcgcact
      601 ggctgcattt tcttggttga atcctccagc cagcaaaata tcataagcag tatcgcagat
      661 agctaagcga tcttcatgag aagataaagt aaaaccgtga gtacctttat ctgaacggat
      721 cacttgataa ttgctttctt ccgaacgacc ataagtgatc gttggaatct ggaatagatc
      781 ggctgtttcc ctaagtaaat caaaatagtc actttcatga tttaaaacaa ctgtttttga
      841 attcaagatc aattgtcgct tgcaatagaa atagtcatca aatgtcggat gttcgatcgg
      901 actgatatgg tcaggcgaga tattcaagaa gattccgaca tctaaagtca agccgtatac
      961 gcgttgtgtt ttgtaagctt gtgaagaaac ttccatcact agatgagtca taccattttc
     1021 aactgcttct gacatcatac gatataaatc taatgactca ggcgttgtca aatgggattt
     1081 gaaatatgtt ttgccatcta acgttgtatt catagtagaa agcaatgctg tttttttatt
     1141 tgttgtgtga tccaagattg cttttgtaaa gtacgcagca gttgttttac cttttgttcc
     1201 agtgaatccg atcactttca atttattttg cggatagtcg taaaaagcca tagcaagaag
     1261 cgccattgct ttacggatat ccgttacgat gattcctact gctgaatttt cgtatggttg
     1321 ttctgataca taatattgaa taccctgttc gattgctgaa tccaaatatt cttctttgaa
     1381 attcattcct ttacagaaaa acaaagtaga agcatcagct tttcttgaat cataacttaa
     1441 ctgaagaaat tcagcttttt ctggtacatc aagatgccag cctcttgttg aaacaaattc
     1501 ttttaataga ttttctttta atagtaaatg acggatgtct tctaaagaga agctcattga
     1561 ttgatccctc gctttattca atttcttggt ttattatagc acactctttt taatatatcg
     1621 tttgtttccg ctatgacaaa atccagcttt attcaaattt tcttgacaca ggggacaagc
     1681 ggatgagtat tttcataaac aatgaactga ctgtaataga taggagaacg aatagtgtct
     1741 ccaacaatcc aaaaacataa taattcaaaa agacgataag actattggta agtaaaagtc
     1801 cttgcggcat tggaagccga aaaaatttgt ggaaaataat cggcggtatc gttataccgc
     1861 catttgaggc cccgactgtg tacaatgtgt aaaccccaag actgaacaaa agacttcctg
     1921 aaatcagtga aaagaaatgt gaggagatca tttccacttt tggaacaatc tctaaaacaa
     1981 caggaaaaag cagacttccc gctacagtct taaaaaatac tgattttcct aataggacag
     2041 cggctagaag cagcatcata agattgatgc tccaaacagt aactgagcgt tggatagaaa
     2101 aggccgtttc aactaaatgt ccgatggcgc ccactccggc ggaagctata tcgtgaggga
     2161 gcaagaacca attcaatcca actgtgataa agaaaagacc taaaaatata acgggtagtt
     2221 tttttatttc ttgtgtcatt gatatctcct ataaatgtta gccataaaag gctgtatatt
     2281 tctttacttt accaaaaaaa cgaaaccgaa ttcaaaaaaa caattgttct tataaaagtt
     2341 atataaaatc gattgccagt ttctgtttta ccaattaaaa tgatagtaat gttttgtaga
     2401 aagttggaat ctgaccgatg gataatcaaa gaaaaccagc attagatcaa ttaacaccgc
     2461 aagaaaaaat ggcacgaggc tctgcttgga tgaccgccag taatatcatt tcaagattgc
     2521 taggcgctgt ctatatcatt ccctggtatg cttggatggg cgaaaatgct aaggctgcca
     2581 atggattatt caatatggga tacaatatat atgcattgtt tctactgatc tctacagcag
     2641 gaatacctgc tgcgattgca aaacaaactg cacgctataa ttcattgaat gaatatggca
     2701 cttcacgacg attatttatc cgtgcattac agatgatggg cgggctaggc ctgttgtttg
     2761 ctttgttcat gtacatcgcc tctccttggc tagctcatgc ttctggtggc ggagaagaac
     2821 tgattccaac gatgcgttct ttgagtatcg ctgtattggt ttttccttgt atgagtgtga
     2881 tacgagggta ttttcaaggc aatcaagaaa tgatgcctta tgcgctgtcg cagattgtag
     2941 aacaggtggc acgtattttt tatatgctat tggctacatt catcatcatg aaagtcatgg
     3001 atggtgacta tgtgacggcg gtcacgcaat caactttcgc ggcatttatc ggtatgctag
     3061 caagtattct tgtccttttg tattttttga agaaacatca agcgtataca tcggcgttta
     3121 ttcactacag cgaaaacaaa ataaatattg caacgaagga attgttgtta gataccgtta
     3181 aagaagcgat tccgtttatt attgtgggtt caggggtaac gatcttcaaa ttagtcgatc
     3241 agttcacttt tatgaagatc atgtctgcat ccaccgagta ttccaatgca cagttactgg
     3301 atcttttttc gatttttagt gccaatccag ataagttgac gatggtagtt attgcattgg
     3361 caacatcgat cgcgtctact gggttaccgt tgattacaga agctgttacg ttgaaagatc
     3421 gagtaggtct ggcaaaattg acaagcggaa atttacagtt gttttcattt tttatgtttc
     3481 cggcgacatt tggtgttatg ttacttgcct atccgctaaa tacattattt tatacaccgg
     3541 attctctagg aagtaatgta ctgatccaag caagttttgt aggactcttc ctaggactat
     3601 atatgctagt ttccaacatg ctgcaaggaa tgtttgaaaa caaggcggca attaattatt
     3661 tggttattgg gtttctagtg aaactccttc ttcaataccc agccattcgt ttatttgaag
     3721 tatacggccc tttattggcg acaatgatcg gatttgctgt gtcttgtacc ttgatcttaa
     3781 agagaatcca tgaagtagcc cgctttaaac caagttttgt ctggcgcagg acattgctga
     3841 tctttattct taccttgatt atgcttttgg cttcgtggat cacaaagatg atttttggtg
     3901 cttttttaaa cgaagatagc aaattccagt ctctattgtt gattcttgta gtagcaggta
     3961 taggggcttt agtgtatggt tatttagctt tgaaaatccg cttggcagaa agactactag
     4021 gaccaggaat agcgcgttta cgacataaat taaaaataaa ataagtgaat cagaagagct
     4081 agattgatgc tcttttgaaa tgggggaaac gcatgcgttt agataagttt ttagcagata
     4141 tgggacttgg cagccgcaaa gaagtcaaag gattgatcaa aaaaggaacg atcgaagtca
     4201 atggacaagt ggtgaaaagc gataaagcgc aagtaaatga attcgaagat gcggttactt
     4261 atttaggaga aatcattacg tatcaaaaag atttttacta cattttgcat aaacctgcag
     4321 gagtcatttc tgctactcag gataatcatg atcaaaccgt aatcgattta ttggaagacc
     4381 aggattatcg ggaagatctt tttccagtcg gccgattaga taaagacaca gagggcctct
     4441 tgattttaac gaatgacggg gtgtttgccc atcagttact atcacctaaa aaacatgtgg
     4501 agaaagaata tctagctgag gttagcggta taatgacaga agaagatatc cgtttatttg
     4561 cagaaggact gattattgat ggcgatgagc agacattgcc ggcaaaactc caaattgatc
     4621 aagtagatca agagacagaa acttcaatta tccgtctgat tttgcatgaa ggaaagtttc
     4681 accaagttaa gcgaatggtc aaagcagtag gaaaagaagt cttatattta aaaagaatcc
     4741 gtatgggaaa attttggctg cctaaagatt tgaataaagg gcaatatcga cccatgacca
     4801 aagaagagct agaacaagtg agagaaaaat aattgaatgg atagctaaaa gaataagcag
     4861 agtagttgta gcgccatctg aaaagcagac ttgtccgtct gactttcgga tggctttttt
     4921 gtgaaatttc ggcttatttc cgtagaagct acttctggaa caccgtttgt tcggaataag
     4981 ggactgtgac aagacttttg ttacagtccc cattgttcct tgtgactcag agttagatag
     5041 taaggacacg atctccctaa tactcaggcc aaaacgctga attcacaacc tctgataaac
     5101 ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
     5161 tttcccgttt ttttctctaa tactcaggcc aaaacgctga tttcacaacc tctcctatac
     5221 tttgtttcat tttccctata ctttgtttat ttattctcat tattgaaagc gctataatta
     5281 cgttgcgaat gaatcgaaag gggtttttag atgaaaaaac gaaaggggtt caatcatttt
     5341 tttatgaatc tatggcgtta taaagcactt gtcctgatgg ctgtaccggg tatgctttgg
     5401 atgatttttt tcttttacat acctgtaatg gcgaatgtcg tagcattcaa aaattttcat
     5461 atttcagcag atgggttctt ggctagcttg agggaaagtc cttgggtggg ttgggaaaat
     5521 ttcaaatttt tattttcttc ggatcaagca tttctcatca ctaaaaatac aattttatat
     5581 aatgtagcat ttatcctttt gaatctgctg atctcagtcg tatttgcaat cattatgagc
     5641 gagttgagaa ataaacgact cgttaaagtt tatcaaacga tgtccttact accttatttt
     5701 ctatcttggg tcattatcag ttactttgtt tacgcttttt taagtcctga caaaggaata
     5761 ttcaatcagt ggatcactgg acatggagga gaagctgtca attggtataa tgaaccgaaa
     5821 tattggccgt ttatcttggt gttcattggt acatggaaag ggattggtta taacagcatt
     5881 atttactttg catctgttat gggaatcgat ccgacttatt atgaagctgc tatggttgat
     5941 ggagctagca aatggcagca gatcaaacat gtgacgatac cacaacttat tccactaatg
     6001 acgattttaa cgattttagc cgtaggaaac attttccgag ctgattttgg cctgttttat
     6061 aacattccta ggaattccgg tgcattatat gatgtgactc aagttttgga tacgtatatc
     6121 tacaatggcc tgacatccac aggagatttt ggtatgacgg ctgctgcagg actttaccaa
     6181 tcagttgtcg gctttgtctt gttgatgatc acaaatacga tcgcccgacg ttttgatagc
     6241 gattctgcat tattctaagg aggaaaaaaa tgaaaaaaaa gaaagtggag agagtccaag
     6301 tccgctcttt caataagacg gctaattttt tctctaatat aatgattgca ctctttgcta
     6361 tttcctgtat tttgccattt atatttgtta tcgccatctc ttttaccgat gagtccatat
     6421 tgtcgactca cgggtacagt ttttggccag aaaagttcag tatatttggt tatacgtact
     6481 tgttcgacca gatgcaggat aaaattttcc aagcgctttt cgtcactgta cttgttactg
     6541 ttttaggtac actgatcaat agcacggcta cttctttata tgcttatgcg atttcacggt
     6601 caaactttcc attccgtcgt ttttttaccg ttttttgttt aattacgatg ttgttctcac
     6661 cagggatggt ggccaattat cttgttatga caaatcttct tcagttaaaa gatacgattt
     6721 gggcattgat cttgccaatg gctgtcagtc ccttcaatat tattgtgatg cgcacattct
     6781 ttaaacgttc ggtctctgat tcgattattg aatcggcacg gatcgatgga gctagcgaac
     6841 tgcgaatatt tgttcagatc gttttaccgt tagctgttcc aggtattgcg acaatcagtt
     6901 tatttgccgc attgggttac tggaatgact ggttcaatgc tttactatac atccaagatg
     6961 ataagctagt tccactacag tatcttttga tgaaaatcca aagtaatatt caatatctga
     7021 cacaaaattc aggagcagga agccaattga caggcggatt agcctcagtt ccaggagaat
     7081 ctgccagaat ggcgatcgta gtcatctcga cgttgcctat tgcgatcagc tatccatttt
     7141 tccagaagca ttttgtcaaa ggtctaacaa tcggcggagt aaaagaataa agaaacaaaa
     7201 atataaatca taaaattagg aggaagaaaa atgaagttat ggaagaaact agcctttaca
     7261 ggtgtttcag cgttgttgtt gggaacatta gcagcatgcg gaggttctgg ttctaaagac
     7321 caggcagaag ctagcaattc ggacactttg cagatgtatc aaatcggaga taaaccagat
     7381 aatttcgatc aattgatgga agtagcaaac aaacgaatcg aaaaagagat cggtgtaaaa
     7441 gtcaatatca attacattgg ctggggcgat tacgaaaaga aaatgaacgt catcatttct
     7501 tccggggaaa attatgatat cgcctttgcg aacaactatg tttccaatgc ccaaaaagga
     7561 gcctttgctg atttgactga attagcgcct aagtacgctg aaaaagctta taatgattta
     7621 gatgaagcat atattaaagg aaatctggtc aatggaaaat tatatgcttt cccagtcaat
     7681 ggaaatgtct ttgctcaaca agtacttact tttaacaaag ctttactaga taaatacgat
     7741 ctttcaattg atggaatcca atcttacgct gatgcagaaa aagtcttgaa ggaattccat
     7801 gaaaaagaac caaacacggc agcttttgcc attggacaag gcttcaaagt ccaagggaac
     7861 tttgattatc ctttagggaa tacgttacca tttgctattg atctggatgg agatgattcg
     7921 aaaatcatca accaatacga taatgaaacg tatatcggac tgttacgtac aatgcatagc
     7981 tggtacaaac aaggactcat tccaagtgac gctgcaacga gcaataagga ttatccacta
     8041 gaaggaaata cttggctaat gcgtggagaa acacaaggac cgtatgatta tggcgataca
     8101 attttgacaa atgcagcaaa acaagaactt gtctcaaaag caattactgt tccactaaaa
     8161 tctacagctc aagcacaaat ggcaaatttt gtcgtatcaa atacttcaaa aaataaagaa
     8221 aaatcagtag aacttctagg gctgcttaac agtgatccag aattattgaa tgggcttgtt
     8281 tgggggatcg agggagaagc atgggaaaaa gtcgatggtt cagatgataa aatcaaattg
     8341 ctagatggtt atcagccaaa tacacatatg tctgcttgga atacaggaaa caacaagatt
     8401 ctatatacac aagagtctat tacggatgac atgattgcca aacgtgatca atcgattgct
     8461 gatgcagaaa cttcaccgat tttaggattc agcttcaaca ctgacagtgt caaaacagaa
     8521 ttaagtaata tttctaatgt catgaatcaa tatttagatg gattgaatac agggactgtt
     8581 gatccagatg aaacattacc gaaattgaaa gacgctttgg acaaggctgg ctatgataag
     8641 gtattgaaag aaatgcaaaa acaatatgat gagttccgac aagaataata atgaataaag
     8701 acaaaggggt tgtgacaaaa gacgtgtcac aacctcttgt tccgaataaa cggtgttcaa
     8761 aagccagttt ctcggtatta agctaaatat atgtaaaaat gtggaaagcc atattcacaa
     8821 aatttttctt actactcgaa actaagcggc tatttcacaa actcttggta aacggtgttc
     8881 aatcagctaa ccctcggtat tcgctcacaa aacgacaaaa tatgaggaac tatttttccg
     8941 tttgtttctc taataatcag gtcaaggtgc tgattgccca accttttttt gtataatgag
     9001 aaagtataaa cttaatgttt ggaggagaag atatgttagg gaaggttttg gaagagttat
     9061 ttatccggat ctgggtcatt ataaaactaa ccttgtattt ctggatatat acatttgccg
     9121 gcgggatcat ctttggatta ggtgcggcat ggaaaacagt caatgaacta ttttatttgt
     9181 acggattcga gtataaagaa atcacaataa aaagaggctg gaatatatat aaaagaaatt
     9241 ttttaagagg caatctgcta tttagtttat ttttatctgg tactgcttta ttgagttaca
     9301 atttatattt atctgttcaa atcaaagggc tcattttttt agctattgat tttattttac
     9361 tttttgcctt gctctgccta tttgctactt atcaatacag tatgatttta gatagtgaat
     9421 atacgatctc tgttcctaat ctgctaaagc taagttttat ttcggttttt tccagctttt
     9481 cgtcttttct aaaaataatt attggtagcg gtataatttt aggcgtgact tggcaattca
     9541 aaggattgat cctgtttggt gtcattggtc ttttgacggt ttggaatggt acgatgacaa
     9601 cacattggcg tgaagaatta gataaacagt tggaaagtta tgaataagtt tcggattcta
     9661 ttttcaaaag gaaccttact aaatcgtctg ttgcggaaat acgcggttat catgatcggt
     9721 gtaacgatga ctgctaccgt tatttttagt atccatactt gggaacaaaa tcaaaaacag
     9781 gcagaaaaca tgacaagtga tgccgttcaa agcacaagca gaatgctgaa tgataaaaca
     9841 accttgagta ggatcatcaa aaaccagctt gtagggaatt ccgaaaagat cgaaaatgtg
     9901 acgacctact taacaaaacc catagatcag tatctgatgt atgtatacga acagcaaaac
     9961 agtaccgatg aattagtttc tttcccaaat caaatcaaag atctctacgc aaattatgaa
    10021 gaactaagtg cgatatatat cgtgttgaat caactaccgg agtattatga atcttctaga
    10081 gaaaacaaag gtggggaaat aaaaaagggg acaccaaaat tactagacgc cttttatatc
    10141 aagttgccta tcacacaagg aggagctgag tcaggaagta tttttattgg ttttcaaaag
    10201 aaagaactag atatgatttt agaaaatcta acgtcattca atggactctc tttatacatg
    10261 atttctggaa cgacaaatcg actatacaca ttccatgata ataaaatagc taaagaaacg
    10321 tttgaaagac aagaaatgat catcacagag agtctgaaga aaaattcaca attgccgatt
    10381 gacgatctgg aaaaaaacaa ttttgttcaa caccaagaac tgtctgatga ttatcgtata
    10441 ttagctgtac tagataaaaa aagtgtaaaa atcgaaacgg gaaaaaatct tgccccgctg
    10501 atcattggta tcattatttt agacaatata ctgcttatct ttctttataa aacattcaaa
    10561 cgatattcac agcaagtatc tatcgtcatg caggccatgg atcaaacagc agcagggaat
    10621 ttagagacgc ggatcgacat aacaaacacg gaatatgaac tgaaggaact ttcaatcggt
    10681 atcaacgaaa tgttggatag catcaatcag tttgtagaag acatctacaa attagaaatc
    10741 aaacagcaag acgcacatat gcgggcgctt caagcacaga tcaatccaca ctttttatat
    10801 aatacattag aatatatccg aatgtatgca atcagcgaag gaagtgaaga attagcagat
    10861 gttgtgtatg cgttttccgc tttgcttcga aacaatacga atcaagagaa aacgatcact
    10921 ttaaaagaag aactggactt ttgcgaaaaa tatgtctatt tgtatcaaat gcgttatcct
    10981 aatcgagtag cctatcattt tatgatcgat cctgatttgg aaaagatcga agtacctaaa
    11041 tttgtgatcc agccattagt tgaaaattat tttaaacacg gtattgattt tactcgattc
    11101 gataatgcgc taagtgtcaa agtcttgcag gaaggcaaac gggtaaggat catcatcaaa
    11161 gataatggaa aagggatgac tgaaaaacgg ctaaaacaga ttgaagaaaa actgtctcat
    11221 cctaaagtgg aacttcacgg ttctatcggt ctgcaaaacg tgaatgaacg tttacgtgcc
    11281 agcttcggca gcagttatta tatgtcgctt gagaataatg agacaggtgg actgaccgtt
    11341 tctattacat tcaaggaggg atgagcgtgt atcaagtgtt attggtagat gatgaataca
    11401 tgattttgaa tgggttaaaa aagataattg attggcaaag tttaggcttt caaatagtag
    11461 caacagcaga gaatgccaaa gaaggactag ctgtacttga acagcgacag attgatttag
    11521 tagtaacgga tgtgacaatg ccagaaataa atggactgga atttattgaa gcggctcaaa
    11581 aagaacgtca taattttgaa tttatgattt tatcaggata ccaagaattc gattatttga
    11641 aaggaggtat gcaattaggc gccgtgaatt atttgatgaa acccgtgaat aagttcgaat
    11701 tggttgaaag cctcaagaaa atcaaaacac ggttagatca gcaaaatgaa caaaaaaatc
    11761 aacaagaaat ctatcaagaa ctgttattca gtcaatggtt gaatgaagaa ttggatgaaa
    11821 taagtgaaga agaaataata ggacaaataa gtgagaagca gcataggata gtattgattc
    11881 aactggcaag aactcatgat actcttatca atagatggtt gcaagaacgg caacaaatct
    11941 tttactatca acgaaattac ggagaccgtg tattgatcac gttgttgttg gaggcagatg
    12001 aagttgatgg cttctgttgt tttgttcaag aaaactttcc tgatcaggaa tggctgatca
    12061 gtatcgggga agagacagat tctttggaca aaatccctga aacattccaa caagcgaaag
    12121 acaatctaca gttgcatcaa ttttatggag ataaagacga gcatgttttt tacgcagtac
    12181 aagcagaaac aaaagatcaa accatcgatt tttcaagttt cagacgcgtg ctgcaaaata
    12241 aacaattgga agtcgctcaa aaaatgatca ctgatttttt tgaacagttt cagttagcag
    12301 ccatgatgcc ggaagatata cgatattcca gttttttact gtttatggag attcaacggg
    12361 aattgattga tctggaagat gaggaatatt tacaaggaat cgaaaaaatc catcaggcaa
    12421 aaacggtgca agaacttcag cagctacttc tgtcattcat caaaaagcat cagagacaga
    12481 aaaagtacag tgacaatgtg gaaaaagtaa tcgtaatcct gcaccagcat tatcaagaac
    12541 cacttacact caaagaagtg tcagaaaacc tgcatttaaa tgtcatgtat ctaggacagc
    12601 tgttcaaaaa agaaacgaaa aaaagttttt cagcttattt gaaccattta cgtatggaaa
    12661 aagcgaaaca actgcttttg cacagtaatc aaaacatcaa tgagatagca agtgagattg
    12721 gctacaacaa caccacgtat ttctctaaat tattcaagaa aatcgtcggc agatccccta
    12781 aagagtaccg agaaaacagt ggagaatcat aaaaaaaaaa gccaatgtaa tgaaataact
    12841 tataaaaaat tgtctagaaa tctaattttg ggctgttctt attacttgag gcaaggcagc
    12901 tttttcatga cctctcgata aacgg
//
LOCUS       DAYYCW010000082        12733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000082 DAYYCW010000000
VERSION     DAYYCW010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.084"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACHAYA_002123"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACHAYA_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8605910.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACHAYA_002124"
     CDS             complement(323..892)
                     /locus_tag="ACHAYA_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM8605911.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACHAYA_002125"
     CDS             1078..1980
                     /locus_tag="ACHAYA_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8605912.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACHAYA_002126"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACHAYA_002126"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM8605913.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACHAYA_002127"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACHAYA_002127"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM8605914.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACHAYA_002128"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACHAYA_002128"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM8605915.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="ACHAYA_002129"
     CDS             8548..8733
                     /locus_tag="ACHAYA_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM8605916.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="ACHAYA_002130"
     CDS             complement(8795..9991)
                     /locus_tag="ACHAYA_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8605917.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="ACHAYA_002131"
     CDS             complement(10161..11597)
                     /locus_tag="ACHAYA_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8605918.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="ACHAYA_002132"
     CDS             11890..12699
                     /locus_tag="ACHAYA_002132"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8605919.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3801 a         2672 c         2250 g         4010 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tga
//
LOCUS       DAYYCW010000083        12405 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000083 DAYYCW010000000
VERSION     DAYYCW010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.085"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(148..2223)
                     /gene="glyS"
                     /locus_tag="ACHAYA_002133"
     CDS             complement(148..2223)
                     /gene="glyS"
                     /locus_tag="ACHAYA_002133"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM8605920.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2225..3142)
                     /gene="glyQ"
                     /locus_tag="ACHAYA_002134"
     CDS             complement(2225..3142)
                     /gene="glyQ"
                     /locus_tag="ACHAYA_002134"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM8605921.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3526..4338)
                     /gene="recO"
                     /locus_tag="ACHAYA_002135"
     CDS             complement(3526..4338)
                     /gene="recO"
                     /locus_tag="ACHAYA_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM8605922.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4481..5380)
                     /gene="era"
                     /locus_tag="ACHAYA_002136"
     CDS             complement(4481..5380)
                     /gene="era"
                     /locus_tag="ACHAYA_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM8605923.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5395..5790)
                     /locus_tag="ACHAYA_002137"
     CDS             complement(5395..5790)
                     /locus_tag="ACHAYA_002137"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8605924.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5774..6250)
                     /gene="ybeY"
                     /locus_tag="ACHAYA_002138"
     CDS             complement(5774..6250)
                     /gene="ybeY"
                     /locus_tag="ACHAYA_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM8605925.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6267..8459)
                     /locus_tag="ACHAYA_002139"
     CDS             complement(6267..8459)
                     /locus_tag="ACHAYA_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM8605926.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8483..9454)
                     /locus_tag="ACHAYA_002140"
     CDS             complement(8483..9454)
                     /locus_tag="ACHAYA_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM8605927.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10160..11539
                     /gene="brnQ"
                     /locus_tag="ACHAYA_002141"
     CDS             10160..11539
                     /gene="brnQ"
                     /locus_tag="ACHAYA_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8605928.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            complement(11693..12139)
                     /locus_tag="ACHAYA_002142"
     CDS             complement(11693..12139)
                     /locus_tag="ACHAYA_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM8605929.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12166..12342)
                     /gene="rpsU"
                     /locus_tag="ACHAYA_002143"
     CDS             complement(12166..12342)
                     /gene="rpsU"
                     /locus_tag="ACHAYA_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM8605930.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
BASE COUNT         3349 a         2597 c         2252 g         4207 t
ORIGIN      
        1 ttcataggtg tttttaaaag ttacacaaaa aataaaataa caaaaaaatt aacattgaat
       61 taataatagt tatttatatt tataacaaat aaaccagaga aatagcctat tttgtgcctg
      121 ttctctggtt tatttacata ttagaaatta ttttgtgatg atttgatcta aacttgctag
      181 tgacaaagcc atggcagcaa tttgacttaa ttgcgttaat cggttcttac gtactgctgg
      241 ttcttctgcc atgaccatcg tctgatcaaa ataattttcg atcagtgggc gaaggcttac
      301 aagctgatca taattttcgt tgatcgtttg gtcagcaaag cttgttttca ttgcctctac
      361 tgctttgtgc agttctttct ccgcttcgtt ttcgaacagt tcagggtcta cttctgattg
      421 tttttcaagc tccgcttttt tcgctaagtt cacgacacgt gtcaaagctt ccatcgatgg
      481 tttgaagtct tcatctgcaa gatgctgctt gaacaattgt gctgctgcaa atacttttgt
      541 cagatctttt tgttctgcat tcaaaacagc ttcgatgaca tcatgacgga tattcttcgt
      601 tgtcaataat tggcgcagac ggcctttgat aaagtcgatc acctgttgtt gtcccgctga
      661 caagccaatg ccaaaacgat ccgtatcttt actgataact tcactaatcg tttcttgcag
      721 aacagacaaa ggaaaggccc attttttcga ttcgatgatt cgaacgacac catatgcttg
      781 acggcgcaaa gcatatggat cgtttgagcc acttgggatc atcccaactg caaagaaaga
      841 aaagatacta tctaatttat cagcaatcgc taaaacagaa cctactgtag tctcaggtaa
      901 atctccttca ctggatatag gcatgtaatg ttcacggatc gctgttgcta cggctggttt
      961 ctcgccttta agcagagcat atttttcgcc catgatccct tgtagttcag ggaattctcc
     1021 taccatgttg gttactagat caaatttata gatttcagca gcgcgttgca gatccatcaa
     1081 ttcttcctca gatagtccta cttgttcgcc tatgatgcga gcgatgactt gtacccgttg
     1141 cattttctca tagatgctac cgattttttc atggaaagta acagttttta atttttcaac
     1201 gtattggtcg attgaagctt tcttgtcttc attataaaag aattccgcat cttctaaacg
     1261 agcagtcaat actttttgat tgcctcgtac cacattttcc aattttaccc gatcgccatt
     1321 acgagcagaa ataaaatgag gaagcaacat accttgatcg ttccgtactt cgaaataacg
     1381 ttgatgttct ttcatagaag tgactagtac ttcttcagga acagatagat atttttcttc
     1441 gaagtcgcca gaaaagacag ttggatattc gactaaattg ttgacttctt ctagtaaatc
     1501 ttcatctaga tcaatgatcc agtgattttt ttctgctaat tcgtttgctt gtgtgatgat
     1561 catttctttt cttttttgcg gtgaagcaat aacaaattgt tcattcaatt tttcttcgta
     1621 ttgttttgca tgcgtaaatg tcacatcttc gcctaagaaa cgatgacctc ttgaagctct
     1681 gtctgttttg acatccaaga tctcaaacgg gattatttcg tcatctaaca atgccacgat
     1741 ccagtgaata gggcgaatat attcaaaatc gtattctgcc cagtgcatcg ttactgggaa
     1801 agtgaggctt ttaatgacat ctgctaaacc tgtcaatact tcttttgctt ctttgccgtg
     1861 agtgtatttc gttacataga catattccac gccttttaat tctctaaaag tgatagcatc
     1921 tgtcgtcaga ccttgtccgc ggacaaatcc ttcagctgct ttcgtccagt ttccatctgc
     1981 gtccaaagca atttttttgg ctggtccttt gacttcttct tcgctgtctg cttgacgttc
     2041 tggaatgttt gttactttga ttgctaagcg gcgcggtgta gaaaacgttt cgatcgtatc
     2101 ataagtcaat tggttttcat ctaaaaattt tgctgttttt tcttctaatt gtttcatgct
     2161 tggtgtgact acatgagcag gtatttcttc cagcccgatt tcaagtaata agtcttttgc
     2221 catcttattc agcctccttc acgttttctg gatttgctgt gtttttattc aataaaggga
     2281 atcctaattt ttcgcgttct gccacaaaga ttttggctac agcacgagcc atgttgcgga
     2341 tacgggaaag ataacctgca cgctctgtta cggatactgc accgcgagca tctaataaat
     2401 tgaatgtatg gctacatttc aaaatataat catacgctgg atgaacaaga ttttcatcga
     2461 tacagcgttt agcttctttt tcaaaacgat caaaattgct tagtagcagg tcttggtcgc
     2521 ttacttcaaa agaatattta gaatgttcat attctggttg tttgaagatc tcaccatatt
     2581 tcacgccatc agcccattcc agatcataaa cactttcaac ttcttggata taagaagcaa
     2641 gacgttctag tccataagtg atctcagaag taactggttt acaaggtagt cctccgactt
     2701 gctggaagta tgtgaattgt gtgatctcca tcccatcaag ccatacttcc cagcccacac
     2761 cggcacaacc catcgatggg ttctcccagt tgtcttcaac aaagcgaata tcgtgttcta
     2821 atggatcaat tcctaaaaga cgcaagcttt ctaaataaag ctcttggata ttttcaggtg
     2881 aaggtttcat tacgacttga aattgatgat gctgatacaa acggtttggg ttttctccat
     2941 aacgcccgtc agctggtcgg cgggatggct ctacataagc agcattccat ggttccggtc
     3001 cgattgcccg taagaatgta tatgggctca tcgtacctgc tcctttttct gtatcataag
     3061 cttgcatcag catgcagccg tttgatgacc aaaatttttg taatgttaaa atcatatctt
     3121 ggacggtcaa ttttttactc atctgttttg cccctttcaa tttttaagcg ttgctgtcaa
     3181 taagaataca ggaaagaaaa cgacaaaaag tccctatgtc tgattacaga catagggacg
     3241 aataatttcg cggttccacc ctacttccga ataaagattc ggcagcttca tttatcgtac
     3301 tcgcaagtgc ctcttcaaaa atcgtttggg tccggcttcc actctcccga actcgctaaa
     3361 ccacgtgttt tttactgatc ttggtcaatg tacgtattca ttttgcagtt ttctggtttg
     3421 tataatagat cctctcagtg aacgatctat ttaatctacg gttaataata gacggaagtc
     3481 cctgtatttg tcaaatagtt ttgatttttt tgttgtatct ttcatttatt cttctttttc
     3541 aggtatctct ttttcaggca tatttttctc tggcgtcttt aatacgtgtg cccaagactt
     3601 catttgatca atgaactttt tgctttttag atgaataccg acatattcat catacaactg
     3661 gtcaatgagg tcacggattg cctgcttcgt ttcttctttc aagtggattc cactgatctt
     3721 atcataagaa atagccgaaa acattcgaac gaaataaaca gcgcgaggat ctgcatgata
     3781 acggtgttcg tccattggcc agtgtttctc gcatatcaca ccgccatatt tggaagaata
     3841 gtcaaatttt ccttttgttt ccccgcatac gacacagtgc gtccaaattg gcgcgattcc
     3901 gaagcgttgc agtatttgca cttcaaagat attcgtgatg atttctgcat catctccttg
     3961 gtccatccgc tgcagagctt gttgcgtaaa ttggaataaa tgcgggtcat attgatgatc
     4021 ttcgattgct gcatctacta gatttagcaa atacgtacca taaccgctca aaaagatatc
     4081 ttcttggatg ttccgaaacg catggacgtc tttgacactg cttaaaaacg acaaaccttc
     4141 acttcgaaaa ttaccgatat agactgcttc ggtaaagggg agcaaagccg gagctaatgg
     4201 ctgattttgc cgatggattc ctttgatata aaacatcatt ttgccagaag attccgtaaa
     4261 gattttgact aatttatctt tttctttgta attacggcta gataaaatca agcctttcga
     4321 ttctgcaagc tggctcatat tctccctcct aaaaaataaa tctggaaaag cagattctgc
     4381 cgatccagat tatttatgtt tcgatcttcc gctgtttttc tcaacgtacg gatttttatc
     4441 cagtttattt ctatacctaa cctataagaa gtccagaata tcagtactcg tcttttcggt
     4501 agccgaagtc ttgcagatag acttttttgt ctcgccagtc tttttggact ttgacccaaa
     4561 gttctagaaa tactttgtct cctaaaagat tttcgatatc ctttcttgct ttcgttccta
     4621 cttgcttcag catttttccg cctttgccga taatgattcc tttttggcta tctctttcga
     4681 cgatgatcgt ggcctggata tggattttat cattctcgtt tcgcttcatc gaatcaacaa
     4741 cgactgcgac agaatgaggc acttcgtctc gtgttaaaaa caagactttc tcacggatca
     4801 attcagaaac gatgaagtat tcaggatgat cagtcacttg atcatctgga aaatattgtg
     4861 ggccctctgg catttcactg actaatacat ccattaagcg ttcgacattg tttccttctg
     4921 tagcagagat cggaatcact tgtgcaaaag gcatctgcgt cgaataatct tcgatgattc
     4981 ccagcaactc gtctggatgg atcttatcga ttttattgat aatcagataa acaggagaat
     5041 gcacgttttt tagacgttca ataataaaat cgtctccccg gccacgtttt tgatcagcac
     5101 tgatcataaa taagactgca tccacttctt tcaacgcact gtaagctgct tctaccataa
     5161 aatctcctag acgatgcttt ggtttatgga ttcctggtgt atcgataaaa atcagctgag
     5221 cttctggtgt agtatatact ccttggattt tatttctagt tgtctgtgcc ttgtcactca
     5281 taatggcaat tttttgaccc acgattcggt tcaataatgt ggattttcct acattcgggc
     5341 ggccgacgat tgcgacaaac cctgatttat gttctggcat atacgttctc ctttttaatt
     5401 aaacaacaaa tgatatattt tagggacaaa taaaatcaac ccaatgatga cagcaaaaca
     5461 agcggtcact aaaactgctc cagctgccat atctttgatt tttttcccaa tcggatgaaa
     5521 atgaaaatcc gtaaacatgt ccactacatt ttcaaaaaca gtattgatga tctctactat
     5581 ccaaacgaga aaaacagcta aaagcagcca taaccactct atccgatcaa gctggaaaat
     5641 cacgcccata aataaagcaa ggataccgaa cgcaacatgt ttcctcatat tgcgttcttc
     5701 ctcaaaaacg gttttgactc cttggacagc aaattccaca gacatcagga aatgtttgtt
     5761 tttctctacg tttttatctt ttgagtccat aggcatccaa gatctctttc tgtaaaccaa
     5821 acatttcttt ctcatcttct ggtgtcatat ggtcataccc gttgatatgc aagaatccat
     5881 gtaccgctaa aaagccaagt tcccgatcat atgaatggcc gtaagcttct gcttgttcag
     5941 cagcacgttc aatagagatc atgatgtctc ctagtacacg aggtaatgga ttctctccat
     6001 cttcaaaaat cactgggatt tcgtcttcgc cttcttcttc cagagcaaaa ctgatgacgt
     6061 ctgtcggtgc atcttttcca cggtaatcac gattgattac ttggattgcc gcattgtcca
     6121 tgaaagtaac agacatttct gtttcctctg gcagctttaa ataagatgct gcaaattgca
     6181 gcaagtcatc gatttctttc attttctctt ctgagacttg gtttgtttca tccataaagg
     6241 taatatccat tatacatcgc ttctttctat tcttcttgtt cttcctttat cttctcaact
     6301 tctgttttca ttttaggata tttgatccga gaatggaagg tgctacttaa accgctgatt
     6361 aatgattttt ccacttttcg gatttctttc aaggtcagtc cactatcgtc taattgacca
     6421 tcggaaatac gctcttcgat aagattatgg acaaaacttt cgattttttc gctcgtcgga
     6481 tgatccattg cccgtactgc agcctcacaa ctgtcagcaa tattgacgat ccctgcttca
     6541 cgactttgcg gttttggtcc aggataacgg aattgctctt ctgttacttc cggattgcgt
     6601 tctttcgctt tgatgtagaa ataacgcatt agcgtggttc catgatgctg tttgcaaata
     6661 tcgatcacca tttgcggcat attataatct tccaatattt tggctccatc tgtcacatgt
     6721 ccaaagataa tatgtttact gtcttctggc aataagaaat tgtgcgggtt ttccgctcct
     6781 gaaggcaagt tttcaacgaa aaagctagca tgtttgatct tgccgatatc atgataatag
     6841 caagctactc gagttagcaa agaacgtccg ccgatttctg ctacagcgtt agcacttaga
     6901 ttagctacca tcatactatg atgataagta cctggcgctt cttccagcaa ctgtttcaat
     6961 aaaggatgat ttggattgct gagttcattt aatacaatta cactgtcatc attgaccatc
     7021 aattcaatat atgggtgtag accgatcgtc agcaagaatg agaagagtgc accagtgaac
     7081 ccgcagacga gcattaacca agtcgttgca tcaccaaagc tcattccttg atagacgatc
     7141 aaaatgacat ccatcatcac aggaaaaata atgacccaca tggcagcaga taatccttgt
     7201 tctgagatcc gtttgcgtct gacaatcgtt gccatgagcc cagagaaaac ataagacatc
     7261 aagataatcg ttagaaagtt cgtgccaatc gaatcataaa aaatgaaaag tgcggatact
     7321 gcttggaaaa taccagccat gatccctgct cttcgattca agaaaaagct taaaacaagt
     7381 ggtgcaaaag cagcgggata aaacagcggg atataagtag cttgttccgt ttggaataat
     7441 tggaaaaatt tcatcagcac tatgctgact gacatagctg tcacatagaa aagcacgtat
     7501 tttgttcttt ctcctgcatt ttggaattga agtgaattat aaatcagtac gccgatttga
     7561 agcaaaacag ctaaaaccat ggcaattagc ggaaaaatcg aagttgtttg attagtcagt
     7621 cctagtaatt ccaatttttt catagctgaa gcatcgattt gattaccttc acgaacaatg
     7681 atttctcctt ggaagatcat cactggttgt acagcttctc gtgcggattg tttcaattct
     7741 tctgtctttt tctcattcaa tacgtcattc actacgatac cttgatccac taagtagcga
     7801 atcatttgtt gttgtgctgg tgtgacattc aaatattgga tttgctcttc tgcttcttgt
     7861 ttaaaagctt ccaattcact ttcacgaact tgtttgctca tctgttcgtc aatgagcttc
     7921 agactctcat ccctcacagt atgaatctca gtttcagtca tttcaaatat cgtcgaataa
     7981 aaattgttcg gtagtttttg ataaaacaca acattttctg cattcacatt ttcaaaattt
     8041 ttcttcagcg cggcgatccg ctcttctgta gtaggctttg ggatattttc atcttccttt
     8101 gccttatcaa tcttttcgtc ataagatttg ttgatggcat cattcgtctt atcgatcaat
     8161 tcaaatagtt gcttgatccg attgttctgg tcatctgcaa gatctttttg gtaagtgtat
     8221 tctggagtga ctgcttctgc tgccagtttt cttttttgtt cggtttcttc cgtgttctcg
     8281 atcgttttgt tcgcacggat actttcttct gctacttggc cttctctgaa atcattggcc
     8341 ttttgtttga cactactaaa tgtaacgccg cagataatga taaaaaacaa gaacagcagg
     8401 aaaggcaatg ctgcctttcc aagtttttct tgtagtttct gcagtgtttt attcaacatg
     8461 tgcttatcct cctaagcaaa gcttattctt gatggagatc tgccttttca taagctttga
     8521 tgatttcggc aactacaggg tgccgaacaa cgtcacttgc ttcaaaatta acaaaatcga
     8581 ttttcttgat ttgctttaat gttcgttctg catgaacaag accacttgtc gtccctcgag
     8641 gcaagtctat ctgacttgta tccccattga caatcatttt tgattgataa ccgagacggg
     8701 tcaagaacat tttcatctga gcgactgtcg tgttttgtgc ttcgtccaag ataacgaatg
     8761 catcatccaa tgttcgcccc cgcatgtaag ctaaaggtgc gatttcgatt acgcctcgtt
     8821 ccatcaatcg gttcgtatga tccaatccaa aaatttggta taaagcatca taaacaggcc
     8881 gcagataagg atccactttt tcttttagat ccccgggaag aaagcctaga ttttcacctg
     8941 cttccacagc aggccgcgtc aaaatgattt tttgcacttc accttttttc aaggcagcga
     9001 ttgctaatac tactgcaaga aaagtttttc ctgtaccggc tggtcctacg ccaaatacga
     9061 tatcatgttt ggctaccgat tcgacatatt tcttttggcc gctgtttttt acccgtatag
     9121 gttttccatt acggtctttg acgatctctt cctcatacat ctcaacaaaa taatctaatg
     9181 tacctttttg ccccattttc aatgccgtca caacatcggg cgtacttacg tggattcctc
     9241 gtttgatcaa ttcctgtaat gtccgaatga ctgaggcagc caaggaaatc tgggattgct
     9301 caccaatgat ctgaatgatt tctccacgtg tatggatggt tgtttccgtt gtttcctcaa
     9361 tcagttttat atgcttgtcg tgtgcaccta aaagcatgct gacatcatca gcagccgtca
     9421 atctaatctc taaagaacta gactcttccg tcaaaaatag acatcccctt tttttcgatt
     9481 cattctttcc tattttacca tactcagaag aaactatata gcaaataaga aaaggttcta
     9541 aaagagttgc ttaaccataa gtaccaagaa acgttaaaaa actagccgct tccattctct
     9601 aaaggtctcg tttgaataaa aaaagctgat catgtaagct atgcataaga tagaggacaa
     9661 gtattccgtc taacttagca agtattctct gggtattttt cttgtttcaa aaaaagaaaa
     9721 caaaatattt gaaatattct tgcatttcaa aaaaaacagg tgtattataa cgtcaacaaa
     9781 taaatataca gcaaagcgtc gaggaaaaca gtaggctatt cagtgattta ttcccagtga
     9841 gtcatgtaag tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt
     9901 cgaaaacgta gatcattgtg gctgcctcca ttatcaggct agcggataac tttatcgaaa
     9961 agcgtccgtg aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta
    10021 gagccctttt cccaagtgga aaagggcttt ttttatattt taaggtggtg gttttcatgg
    10081 aatctgatat tagtggtatt tctagtttct gcaacgttta ttttgttact caaaatgata
    10141 attattggag gaaaaattta tggaagaaac aaaaacttat tcatggaagc aattattgat
    10201 ggtcagttca cttatcttcg ggatgttctt cgggtcaggt aatctgatct ttcctgtaca
    10261 cttaggtcaa atggctggat caaactggat cagcgctggt gtaggatttg ccatctcagg
    10321 cgctcttttc ccattattag ctatcttagc tgtagtagtg acaaaaagtg atggtctgta
    10381 tgacttagct aaacctgtcg gaaaatggta tgctgccttg ttcttgatcc ttgttcattt
    10441 aacgatcgga ccattctttg gtacaccgcg gacagcagca actgcttttg aaatggctgc
    10501 taaacccttt ttgcctgaaa aatatgattc attaggtatg tttttattct ctgctctttt
    10561 ctttttactt gcttatttcc ttactgtaca tccaacacgt ttggtcaaat atgtcggaaa
    10621 gtatttaaac tccttgtttc ttgcattatt agctatcatt tttgtcgttt ctttattcca
    10681 acccatgggg aatctaaacc aatctgctgc agattcttat cagacgaacg ctttgacaaa
    10741 tggcgtttta gaaggataca acacggttga tgcagtagct ttacttgctt taagtgtcac
    10801 ttttgtccat gcagttcgtg atcttggttt taaagataaa aaagtcacag aattaacagc
    10861 aaaagctggt agcctgagcg ttgcgttaga agtcatgatt tattttggtc tggtccttct
    10921 cggtgcgatg agtttgaata agatgtcttt atcagaaaat ggcggaactg cactttctca
    10981 gatcactaat ttttatttag gtaacttcgg tgctagcttc ttaggggtaa tggtcacatt
    11041 aggtgtcttc actactgcta tgggactcgt tgtttctttt gcacaagatt tccataaact
    11101 gttccctaaa gtcagttata tgacatggct gcggttaacg acttttgtct cttttgtcgt
    11161 tgcgaatgcc ggattggata atatcatcca atggtcttta ccagtattga tgcttttgta
    11221 tccattgtca cttgcgttaa tcttactttc cttaacagca aaattcttcc aaaagagtcc
    11281 atttgtctat caagtaacga tgttcttttc tgccgttcca gccgtattgg atatgttggc
    11341 aaattcgcct gcattagtga gtcagcaaac tgtcgtagca agcttactag gattttatca
    11401 tcattatgtt ccttttgctg cattaggtct tggctgggtc gtcccaacgt tgcttggtta
    11461 cgctggcagc ttgctctttt attatgtata tcgcgtatca ggatataaac aagaagcaag
    11521 tgagttacca gaagaataac caaatcaaac acaatttaac ataagagata ttaaacagga
    11581 aaaaaacagc tagaagaaag aataagattc tttcttctag ctgtttttta gttctttttt
    11641 tacgaacaca aaaaactgcg gataatcact gtccgcagtt taaatgataa aattaagatt
    11701 gtaatagttc tttcacaatc gcattcactt ggtttccatc tgcttttcct ttgactttag
    11761 gcataattgc acccataact ttgccaaatt ctttcattga tgaagcacca gtttgagtga
    11821 ttgcttcttg aacaagttgt cgaatttctt cgtcggaaag ttgttccggc atatattttt
    11881 cgactattac aatttcactt tttacttttt cagctaaatc gtcacgtcct gcttcttcaa
    11941 actcatgcaa agaatcacgt cgctgtttca tttcacgaga caacaccgtt agttcttctt
    12001 cttctgtcaa gtcgtgacct tctttaatct gctcgttttg tatagatgac ttgatcattc
    12061 ggatgacttg taaagattcc ttatctttcg ctttcatcgc tgctttcatg tcatcgttta
    12121 atctacttag tagtgacaca ctaccaactc caattcatca cagaactaga attttttgcg
    12181 ttttctagct gcttcagatt ttttcttgcg tcttacactt ggtttttcgt agaattcacg
    12241 tttacgagat tcttgtaaag tacccgcttt tgacacggaa cgtttgaagc gacgaagagc
    12301 atcgtcaagt gattcgtttt tgcgaacgac tgtttttgac atgttaattc cctccctccg
    12361 agcttaaaaa gtaaccgtaa ttgttattga aatatatctc aaaaa
//
LOCUS       DAYYCW010000084        12135 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000084 DAYYCW010000000
VERSION     DAYYCW010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.086"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..610
                     /locus_tag="ACHAYA_002144"
     CDS             <1..610
                     /locus_tag="ACHAYA_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8605931.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACHAYA_002145"
     CDS             723..1001
                     /locus_tag="ACHAYA_002145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8605932.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACHAYA_002146"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACHAYA_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM8605933.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACHAYA_002147"
     CDS             1458..2213
                     /locus_tag="ACHAYA_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8605934.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACHAYA_002148"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACHAYA_002148"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM8605935.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACHAYA_002149"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACHAYA_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM8605936.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACHAYA_002150"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACHAYA_002150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM8605937.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACHAYA_002151"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACHAYA_002151"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM8605938.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACHAYA_002152"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACHAYA_002152"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM8605939.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACHAYA_002153"
     CDS             6950..7492
                     /locus_tag="ACHAYA_002153"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM8605940.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACHAYA_002154"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACHAYA_002154"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM8605941.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACHAYA_002155"
     CDS             9085..9987
                     /locus_tag="ACHAYA_002155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM8605942.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACHAYA_002156"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACHAYA_002156"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM8605943.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACHAYA_002157"
     CDS             11430..11852
                     /locus_tag="ACHAYA_002157"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM8605944.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4035 a         2155 c         2677 g         3268 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttatt
//
LOCUS       DAYYCW010000085        11908 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000085 DAYYCW010000000
VERSION     DAYYCW010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.087"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..465
                     /locus_tag="ACHAYA_002158"
     CDS             <1..465
                     /locus_tag="ACHAYA_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFM8605945.1"
                     /translation="WKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAF
                     LKRLVKQFDEPKVVVTDKAPSIASAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVK
                     RRNKFYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPD"
     gene            complement(1080..2120)
                     /locus_tag="ACHAYA_002159"
     CDS             complement(1080..2120)
                     /locus_tag="ACHAYA_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8605946.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2469..2795)
                     /locus_tag="ACHAYA_002160"
     CDS             complement(2469..2795)
                     /locus_tag="ACHAYA_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605947.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2782..3570)
                     /locus_tag="ACHAYA_002161"
     CDS             complement(2782..3570)
                     /locus_tag="ACHAYA_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8605948.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3938..4228)
                     /locus_tag="ACHAYA_002162"
     CDS             complement(3938..4228)
                     /locus_tag="ACHAYA_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8605949.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4364..4543)
                     /locus_tag="ACHAYA_002163"
     CDS             complement(4364..4543)
                     /locus_tag="ACHAYA_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605950.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4540..4890)
                     /locus_tag="ACHAYA_002164"
     CDS             complement(4540..4890)
                     /locus_tag="ACHAYA_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605951.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4883..6208)
                     /locus_tag="ACHAYA_002165"
     CDS             complement(4883..6208)
                     /locus_tag="ACHAYA_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8605952.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6658..7272
                     /locus_tag="ACHAYA_002166"
     CDS             6658..7272
                     /locus_tag="ACHAYA_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8605953.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(7437..7679)
                     /locus_tag="ACHAYA_002167"
                     /pseudo
     CDS             complement(7437..7679)
                     /locus_tag="ACHAYA_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7883..9202)
                     /locus_tag="ACHAYA_002168"
     CDS             complement(7883..9202)
                     /locus_tag="ACHAYA_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFM8605954.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(9336..9944)
                     /locus_tag="ACHAYA_002169"
     CDS             complement(9336..9944)
                     /locus_tag="ACHAYA_002169"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605955.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSLNNSYF"
     gene            complement(10582..10839)
                     /locus_tag="ACHAYA_002170"
     CDS             complement(10582..10839)
                     /locus_tag="ACHAYA_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8605956.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(10832..11101)
                     /locus_tag="ACHAYA_002171"
     CDS             complement(10832..11101)
                     /locus_tag="ACHAYA_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8605957.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(11232..11411)
                     /locus_tag="ACHAYA_002172"
     CDS             complement(11232..11411)
                     /locus_tag="ACHAYA_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605958.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            11509..11745
                     /locus_tag="ACHAYA_002173"
     CDS             11509..11745
                     /locus_tag="ACHAYA_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605959.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
BASE COUNT         3675 a         2151 c         1826 g         4256 t
ORIGIN      
        1 tggaaaatgg atgaaacgta catcaaaatt aaaggaaaat ggcattattt gtatcgagcc
       61 atcgatgcag atggtttaac cttggatatt tggttacgta aaaaacggga cacacaagca
      121 gcctatgctt ttcttaagcg gttagtgaag cagtttgatg aaccgaaggt tgtagtcaca
      181 gataaagcac catcaattgc tagtgccttt aagaaactaa aagaatacgg attttatcaa
      241 gggacagaac atcgtaccat taaatacctg aataatttga ttgaacaaga ccatcgtcca
      301 gtaaagagac gcaataaatt ctatcgaagt ttacgcactg cctcacccac gattaaaggc
      361 atggaagcca tccgaggatt atataagaaa acccgaaaag aaggcactct cttcgggttt
      421 tcggtctgta ctgaaatcaa ggtattattg ggaatcccag attaaatcat agataccgta
      481 agggatttta ttctttattt aaaactttgc aacagaaccg tccgttgcgg gtaataccgt
      541 aacactgaag ggcacgcatt aaggcgtcat cttaatgctt tcctgtagga cactgacgga
      601 gattaccagt ctcccgagcc ttacaggggc ctttcttttt ttgtattttc aattatcaca
      661 atttatcgtc caaaagtgca gaacgaaaat caattcatca caagcatatc agacaattaa
      721 atttaaaaca actaaaaata tacaaaaaag tgtaattgaa tattagcagc actgcctgaa
      781 aagaagtaat atcaggcttt tcagcttggg gaaataagtg aattacctct cttcatgaat
      841 tattataact caaaaaagca catatgcaaa cactagttcc ctattttttt gaatgaaaaa
      901 taactaaaat atctaacgat tatgacttta aaacctacgt ttgcgattgt tttcataaac
      961 ttttaatcta aaaactccgg aataatagct atttatttgt aacatttgta ataaaattaa
     1021 aatcataaaa aagttctaat ttaaaattga tcatttttat agtgatcgct ttttatcaac
     1081 tactctagcc aattttccgt actaactgtt ggtaattcgt taaatgtttc tctttgttcg
     1141 tttgcaagct ttgccgcaat agctttttcg acaaaccaat tttttacata aacgaaaatt
     1201 agattctgca aattgtttac tttttcagtt ttttgttttt gataagcttt cagcaaagtc
     1261 atatataatc ctttgtcagc atcaattcca taagattcca gttcttcata aacaacaact
     1321 gtttgctcta attcttcagc ttttttcttt gcattatgaa tagtttggac ggttttttga
     1381 gcgtcttcgt aagtactaga aaatgttttt atcaagttta atacattttc tggtacaaat
     1441 gtatttatac tgtcctcttt catgatgtcg acgaagttat ctagtaaaac ttgattttgt
     1501 aactgatcag tttcagtgtc tattatgtgt ctattagtgt ctatagaatt agaatagaat
     1561 agattgtctg cgattttcgc aattacggtt gagtccttga tatgactgga ttttgccgta
     1621 tttgcgattt tcgcaattac ggtggtatca ttgatttctc gcggttttgc cgtatttgcg
     1681 attttcgcaa ttacggtggt atcagtattg tcttcctctt ccatgatttt aaagacgtct
     1741 tgattcgtta cttctggctt gcctaagtac aataaattag gcatggattc aatcttaccg
     1801 tcacgtttat ttactcttcc tctcttctgt ttcagaagac ctgctttttc taattccttt
     1861 ttgattttag atactttacc ttcgcggcat tgtaacaatt tcattaattc ttcaactgtg
     1921 aatatgaaaa atatattatc atcagaatct acccagttat ttttgactga ataatcaaat
     1981 cgatctttaa gtagcatata agcaattttt gcatcatttg atagatccat gtatttttcg
     2041 ttagtaaaaa ataccttagg catttgataa tatttttcac gataaatttc ttttgatttg
     2101 taataattaa aatcattcat agccaatcct ctttctgtag gatagcgttt tgttcaaaac
     2161 tatgttacaa tgaattaaac aaatatatct atccactata tatttttcta ctgctgataa
     2221 gtcgccaaac tttgttaaag cagtagtctt tttttatctt tttttagttt accttaaaaa
     2281 ataaattaaa gcaaaccgct ctatttcaag gtttaagctc atagtaacta tatcacgata
     2341 ttacaataat attatatcac gatatcgtaa tattacaata tgaagataag cgacttatca
     2401 gctttgatac tactgatttt tataaaaaaa atatcacgat attatgatat cgtgatattg
     2461 aatgtaaatt aaattttatt ttgtttcttt gctagtttat atccttcaat caagtattct
     2521 tcaggtatgg actttaatag cgcatcttct ataacgtctg gtttactttt gtctgattca
     2581 gcaatagtcg tccaatattt gagtgctgac attagaattg gatttatacg aatgctatct
     2641 gctgttctta cactacttcc ttgatctttt atgactttac ttatattttt cttggttaat
     2701 tcgatttctt ctacttcttt tgatttaaaa ttctttttat ttttatttcc tagtaatcta
     2761 cttactgcag cttgactgcg attatcttct gccatcaaaa tcaccaactt cttttaaata
     2821 ttcttctgta aatgctttat agtcatcttc agctgttgag ggtttattaa aattgattag
     2881 atctgatcca gcagcttgcg cttcgtcaac ggttattgtc ctacgaatat aagttttgaa
     2941 aatttttgca ccaatgactc tagaaatttc ggttgctgca gacgtcattt ctttagaaat
     3001 gttataacgc ttattatact tagtaaatag aactcctcgt acttcaagct tgtcgttcgt
     3061 atattctttt accgtttcaa tagtttcact taactttatt agtcctttta atgagaaata
     3121 cgcagcttca actggaatga taatttcatc agaaacagta aatgcgttga ctgtaagcat
     3181 tcctaaactt ggtggacaat caattataat ataatcatag ttgtccttga ttggttgaag
     3241 tactctttta agcctgtgtt cacttcctac ttgagtaaat tccctctctg cagaagaaag
     3301 ggctatgtcg gctggcaaaa catcaattcc tttatagtcc agaatagcat ctgtaatgtc
     3361 agcctcccct tttaatactt cataaattgt aggttggttt gttccatcta tatcgaaacc
     3421 tacattatct gtagcatttc cctgaggatc aatgtccact aaaagtacat ttttaccgta
     3481 aacaaatttc aaacatgctg ctaaattaac gctagtcgta gttttagaaa ctccaccttt
     3541 ttgattggct actgacaata ttcttgccat taaaatatcc cccattcatc ttatattata
     3601 atataattat atcataatat tgtgatattg caatatcgta tatgcattat attgtaatat
     3661 tgtgtgatta ttttgtttta aagatatata tcggttcgga tataaagatt attcatatgt
     3721 tattcgaaat taaaataatc actttttatt actttaaaat tttttattaa tgataagaaa
     3781 tatgttttgc gagcagcaac tagaataatt taaacattac taaactgtct atattgttac
     3841 taataattcc cttcttatag tgcattttct tagaaaaaaa caatattacg atatcatgat
     3901 attaagatat cgtaatatta tacaattgtg attgatatta tatatgtttt tttatttttt
     3961 tatcatgttt aatcttgaag gaatcatatt ctttttctaa agacaggagc ttttcataaa
     4021 catcagacat gtttaaacta gaggccaggc ttaaatcata aacgaatgaa gcaggtaatg
     4081 attttattct acgttgtcgg gttatccagc ttgcaaccgt tccttgtggg taggcatata
     4141 actcacaaaa ttgttctaca gtgattccta aattcttgat aatgtaaaca ttgatcgggt
     4201 gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc tactttcttt tattattgcg
     4261 tttgcaataa tagtttaaca cacgcattat tctagtatct actcctattt gtaaaaacaa
     4321 aaaaggattc atagagataa ctctatagaa tccgtaaaat accttatata tataacggga
     4381 agatataaaa cctaaaatta tgataacctg ctgcagcttc tacatagaaa gagaagcgct
     4441 caacaccaac ctcatttatc aaacgttggc cataacccca aatctcttct gatagctgat
     4501 caaaggttat atactttagc tcatattgct ggataatgtt cattctaatc gactccattt
     4561 attatcctgg taaaattcga tatgacgtat ttcgtcgtag tgaattttgt cttttccaac
     4621 gtagatcccc agctcatcac tacctaagat cttgccaatg atatcttctg gatagtatcc
     4681 ttcagcgttt cgttcttctt tttgaataga tactgatcta ttttttatga ctgcagcgtt
     4741 caatacttcc gcaatctctt cagtcgtcat ttgttctttt gctaaatttt gactggttcg
     4801 ttgttcattg tcggcatcaa tttcttcagt atgatcagat aaataaaacc ctgcccactt
     4861 taaacgtcca cgatcattgt aatcaatcat ttttaattcc cccatttccg cccgcatgac
     4921 caccaacaag tgtgcttcta gtaatgcttc tagcgccttc taacattgaa gaagcatgta
     4981 caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat aattttatct aaattttctt
     5041 cgtcaatttg cttttgtgga tcactaaata aatccagctg aagcgaatcg gtgtacacaa
     5101 gcttgctata ggttattcca acatgcctta cttcttgtcc ttcataatac ttatcaaaca
     5161 agaaaagaca ataattctgc aattcctttg tattatctgt gatagggatc ttcatctggt
     5221 gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat gtgtaaatgg acacaacctg
     5281 ttttacagtt atgctgccta atgcgtatcg ctacttgttc cgccatttct tttaccacca
     5341 gctcgatttc atttcttcta gtataatctc gtggtaacac ttgtgaattt ccatatgatt
     5401 tttcctttgt cggttctggt gggattgcta tatctgttcg atcgatccca ttagcatgga
     5461 agtacagctg taagccaatc acacctagtc tattcttaat cgtataagga ttccagttag
     5521 caagatctct aatgctcata atccccatct gattgagtct ttttttcatt cgagagccaa
     5581 ttccccaaaa gtccgtcatt tcaggaatat tccatacttt ttcaggaacg ttttcgtacg
     5641 tccattccgc aacaaatcct tcattatgct ttgcttcgtt gtctaaggct agtttagcca
     5701 aaaggggatt atctccgaca cctactgtag caatcaatcc tagctcttct ttaatattct
     5761 cctggatcat ttgagccagc ttctttctcc gttgacttcg tgttccttca gtcgtaaaaa
     5821 gattcagtga tcgggtcact tttaagattg attcatcgat cgagtaaatc agtagatctt
     5881 catcagccac atatcttctg aaaatattat ttacctgcat atttcgcttg atatataggt
     5941 tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg ttgtggtaag tcacgtggtc
     6001 tactcacatt tgtaatacca taccgctttt ttgcttcagg agaagaagct aatatcaatc
     6061 ctgaaccagt attatcagct cgactcataa caacaagttc tgttgttaat ggatctaaat
     6121 ttctttctat acattcgaca cttgcgtaaa agcttttcat atcgattaaa aagtaatcat
     6181 ttactggctc ttttgaataa tccatcataa ataacactcc attctttcac attatacaaa
     6241 catatgttcg atattgtaaa gaagaaatag ctaaatacat aaaaaaagaa gcataggtat
     6301 gctatcaccc tgcttcttct tttgatttgt atgatcaatt tattctatca ctttactaat
     6361 taatttgaaa atctaataaa aactctcact ctaaaagagt gagagactgc aaattagagt
     6421 acggactttt ttggagttcc gttctttaat attaacaaat gaataaaatc cttacaacat
     6481 ttgagaataa gttatcagta gacacttaca aaaaattctc atactatttc ctccatcatt
     6541 agtttttgtc tatataaggt ttaccctaaa tagacactgg tataacattg aaaaatatgg
     6601 cttcctcgtg tatgattagg gtgtatttta aatagttgca gaaagtaggt aaaagttttg
     6661 agagttgcat acatacgtgt ctcatcgatc gatcaaaatg aacaacgcca gattgaagaa
     6721 atgaaaaaat tcggtgcaga gagaattttc atcgagaaac aatctggcgc aacgattact
     6781 catcgacctg ttttccaaga agcattagat tacgtccgag atcaagatat ttttattgtg
     6841 gaagctattg atcgacttgg aagaaattat gacgagatca tcgcttcagt caattacttg
     6901 aagaagaaaa atgtgaagct gatcattact agtttaccga tcatggctga agcaattggc
     6961 aatcctctcc tggataaatt cattaaggat ctgatcatcc aaattttggc catgatcgct
     7021 gaacaagaac ggactgaatc gaaacgtcgc caggcacaag gaattaagat cgcaaaagca
     7081 aacggcgtct acaaagggcg gcccaaatta tacagtgcag acgcaaaaga tccccaacgt
     7141 cgccttgttt acaaaagcat cgttgaagat cttaaaaatg gtgttgcgat tgctaagatt
     7201 gctaaagact acaatgttac gagacaaacc gtgtatagaa taaaaaaaga cagcatggtc
     7261 aatgacaaat agttgatcat gctgctttta ttatcttctc atctttaatt tcctacatat
     7321 aattactatc tataaaatta agtcttactc agaacagata aagcataaaa ttccaacaaa
     7381 ccatttttta cttaactaaa taaagagtct gttaatataa tcttcactta tttacgttaa
     7441 tcccctactg tatttgttgg ttccttatct atattttcta caaaatcctt attatcttgt
     7501 gatttagctt cgtataattc tttgtatgtc gacacattgg ttatactaat tcctatagct
     7561 agttcttgag actctacttt tctctctagc tcctttgaca aagtgatttg agatttattt
     7621 ggttccgttg gagtcaattc tatagattct tcataaaaat ctctatacat aaagctatcc
     7681 ctgaataatt catagcttta attctttggc acattttatt gaaatatgta tcactaatat
     7741 ccctatcaac tgattttgac taaaaagtta cttcaaacat tcactgttca aaaatattga
     7801 ggaataaatt ttgggtgtaa ggcattttga gcatacttct ccctggataa cttcccagta
     7861 aaaaaatcgt tagattgttg gattagatcc attgactggc tcgcctcagg cgagcaaaga
     7921 ccctgtagaa ttcattctga tacacatgat aactagataa tttgagatag actcgtctac
     7981 ccgtttggac taatttccca gcaactttca gaaacttcaa tcgaatagaa tgaatggtca
     8041 ttcccttttg gacttcttca aagccaatcg ttcttaagaa gttgactaag ttgtaagcta
     8101 tcaagctgag catcattcgg acatgattct ccaaaaagcg aggactgtct gttttgtcaa
     8161 agtaaaagcc agctttcgct tctttaatga agttctccat tgtgccacgt ttggcataga
     8221 tagaaaagat ggtatcagga gaaacatttt ctgatagatt cgtcacgata aattcatgtc
     8281 gaaagagtag ttcgcccgct tcacgtgttg agcgtataca cacgcgacga ctttgtgacc
     8341 aggattgtgc ttgataagtg gtcgagaagt actgaacttc tcgttcttcc cacttttgat
     8401 tatcgccata aagtactgat ttctcagcta tttgacctag tctacgatta ttcttcagtc
     8461 gaataacata atgactcttt tttgattcac acgaatcata cacaccaggt gtagcgaacc
     8521 cgctgtctcc acgaaccaag atgtcagtgt ttggtagaga gtgattatag tgctctaata
     8581 aaggtgtgag aaactccttt acgccttttg atgtatattg atttcctgaa cgtagttcag
     8641 cttttaagaa gtcaccagtc aatccgtcaa aagctaccaa tggatggtaa ccataggttt
     8701 ggtagtgtgt attataattc gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat
     8761 ctaaatcaat gattaattcc gtatcattac ggatgaggcg tgctttatcg ataagttctt
     8821 ggttcaaagc ttgaagttca tggatattct cctcagatag tcgatctaaa aatcgggaaa
     8881 gtgaagattg agaagcgagt tctttcctat ctaaaacagc tttaaacaca ggatcttgtc
     8941 tcaggagatt agctgcagaa tcagctgagt aaccagcaat taattgcata atgaactgct
     9001 caagtatgga taagttgtca tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt
     9061 gcttagccag ttcagaaaaa ccaaagatgt tcatcagctc tttcactagg acgagacccg
     9121 aatcactcga taattgacca cctgtatgag aaatagtgat atttgaattg aattttactt
     9181 ggtttttgtg taagctagtc atgaaaagaa ctcctttctt ttggttggtt tagtcacttt
     9241 aaccatagca gaaaggggtt cttttttcat cacttaacgg gtgaagatga aaaagtaatt
     9301 gtaagctgcc gtgaggcagt ttcacatggt tttaattaaa agtatgaatt attcaggcta
     9361 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     9421 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     9481 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     9541 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     9601 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     9661 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     9721 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     9781 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     9841 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     9901 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     9961 aaatcagcta atttttcttt agaataaaaa aaaacattta tatcagaatc aataaattct
    10021 atttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
    10081 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
    10141 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
    10201 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
    10261 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
    10321 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
    10381 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
    10441 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
    10501 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
    10561 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
    10621 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
    10681 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
    10741 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
    10801 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
    10861 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
    10921 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
    10981 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
    11041 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
    11101 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
    11161 aaatgattaa tattctaact tcttataatt aaaagtgcta tcacttttag tttttaggat
    11221 aaaaagtgac gtcacttttt gagtgctggt acctcaaatc ctgctttttc ttgggcaatc
    11281 aataaatcta acgcaataca cataatcctg gattcacttt tgccgtactt ctgctttagt
    11341 tgctgcaatt ctttttgtct cttttcatcc aacgtaacgg gtaaccgttt atttcctatt
    11401 gcaattggca ttagtactct ctcctttcat cgaaataacc ctatgaaaaa tatggctgca
    11461 atcatcttga atagtagact cacaagggct aatacaaagc gccaaaagat caaacaaaat
    11521 accagtattg ccaaaaatac tacgaagttt aaatgcacag ctgcagcatg gcgcataagt
    11581 atttcgccaa caaataggac taacaataga ggaatgattt tattcatatg gatctccttc
    11641 tttctcttag gttatcgggt gttgcccgct tatttcgcac cacctaagcg gcgggggcgg
    11701 ttggagcgat tggtgcgaaa taagaaccat tcccttagtt actaggtttt ttgtccttat
    11761 ttcacacatt ctcacttttc ggtataaatg attattgcct ttttttcctt tcaaatcgtt
    11821 gatatggcat tcgtttacgg tcaagttcat tttggatttc ttctgtatcg tcgtaagtat
    11881 aggtgtctaa aaaatcagtt aaagtcgt
//
LOCUS       DAYYCW010000086        11735 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000086 DAYYCW010000000
VERSION     DAYYCW010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.088"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            293..562
                     /gene="rpsN"
                     /locus_tag="ACHAYA_002174"
     CDS             293..562
                     /gene="rpsN"
                     /locus_tag="ACHAYA_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8605960.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            577..756
                     /gene="rpmF"
                     /locus_tag="ACHAYA_002175"
     CDS             577..756
                     /gene="rpmF"
                     /locus_tag="ACHAYA_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8605961.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            788..1165
                     /locus_tag="ACHAYA_002176"
     CDS             788..1165
                     /locus_tag="ACHAYA_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605962.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1186..1335
                     /gene="rpmG"
                     /locus_tag="ACHAYA_002177"
     CDS             1186..1335
                     /gene="rpmG"
                     /locus_tag="ACHAYA_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8605963.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1359..2342
                     /locus_tag="ACHAYA_002178"
     CDS             1359..2342
                     /locus_tag="ACHAYA_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8605964.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2372..2494
                     /locus_tag="ACHAYA_002179"
     CDS             2372..2494
                     /locus_tag="ACHAYA_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8605965.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2512..4035
                     /locus_tag="ACHAYA_002180"
     CDS             2512..4035
                     /locus_tag="ACHAYA_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8605966.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4059..4298
                     /locus_tag="ACHAYA_002181"
     CDS             4059..4298
                     /locus_tag="ACHAYA_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8605967.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4491..4952)
                     /locus_tag="ACHAYA_002182"
     CDS             complement(4491..4952)
                     /locus_tag="ACHAYA_002182"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8605968.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5147..5365)
                     /locus_tag="ACHAYA_002183"
     CDS             complement(5147..5365)
                     /locus_tag="ACHAYA_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8605969.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5483..7099
                     /locus_tag="ACHAYA_002184"
     CDS             5483..7099
                     /locus_tag="ACHAYA_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8605970.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7340..8680)
                     /locus_tag="ACHAYA_002185"
                     /pseudo
     CDS             complement(7340..8680)
                     /locus_tag="ACHAYA_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9118..9264)
                     /locus_tag="ACHAYA_002186"
     CDS             complement(9118..9264)
                     /locus_tag="ACHAYA_002186"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605971.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9303..9497)
                     /locus_tag="ACHAYA_002187"
     CDS             complement(9303..9497)
                     /locus_tag="ACHAYA_002187"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8605972.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9407..9739)
                     /locus_tag="ACHAYA_002188"
     CDS             complement(9407..9739)
                     /locus_tag="ACHAYA_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8605973.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10253..10444)
                     /locus_tag="ACHAYA_002189"
     CDS             complement(10253..10444)
                     /locus_tag="ACHAYA_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8605974.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10538..10924)
                     /locus_tag="ACHAYA_002190"
     CDS             complement(10538..10924)
                     /locus_tag="ACHAYA_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8605975.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3910 a         1830 c         2249 g         3746 t
ORIGIN      
        1 catattgccg ttttaagtag aggaaaagct acttaaaacg gtatttgtgt ttgattggac
       61 taataaaagt gaattcttat ttttcagagc ctaaactgca tattctttta aatgatttga
      121 ttatctctat gtaccgataa attcgttttc aaatagttat ctattcatat gagttttatt
      181 tcatgttatt tattgttgac aagagcaact cttgttggta aagtatcagt gtcaaaacgt
      241 aacgattacg atttgaggtg tctaaatcaa aataaaagga gtataaaggt atatggcaaa
      301 aaagtcgaaa attgctaaag caaagaaaca aatggcgatg attgaaaagt atgcagataa
      361 acgtcaggaa ttaaaagccg caggagatcg caccgctctc gctaaattgc caagagattc
      421 aaatccgaat cgtctgaggc ttcgtgatca gacggatggt cgtccaagag ggtatatgcg
      481 aaagttcggt atgtcccgta ttaagtttag agaacttgcc catcaagggt tgattcctgg
      541 agtaaaaaaa gcaagttggt aaaaggagga aatatcatgg cagtaccagc aagaaaaaca
      601 tctaaggcga agaaacggtt aagaagaact catcaaactg tagtaaaacc agaaatttca
      661 tttgacgaag cgaatggtga ttatagacgt agccatcatg tatctttaaa agggtactat
      721 aatggaaaga aagtaattaa aggagcttca aaatagttta atatataact tttaggaggg
      781 aatcaaaatg gaaattattt acccaccttt agttgaagaa ggattaaagt attatcttga
      841 aacgacccag caatcgctag ataaatgcac tttttatcgt tcgatggtag agagaggaat
      901 tattactgaa acgggtttac cgacccaaca ggcaattgaa aatggattag tcaaagatta
      961 ctatgaggac caaggcttat catttgatga ttttttaaga atctatccaa tttttgaaga
     1021 gtatgacgag gagttatttc aatgtatcga tggttattgg gagatacctg ttgatatgaa
     1081 agaaaattta gtttcacaat tggaatctgg cgagttaact tttgaagatg cacaacaaat
     1141 tcaagcatat cttgaagatc gataagtatt gaaggaggaa gatttatgcg tcaaaatatt
     1201 attttggaat gtgtcgaaac tggagaacgt ctgtatctca ccagcaaaaa taaacggaat
     1261 aaccctgaac ggttagaatt aaaaaagtat tcgccaaaat tgcgcagacg ggcaattttt
     1321 aaagaagtga aatagctaag tggtaaaaag gtgacaaaat gggcatacca gttacaatcg
     1381 tttctgggtt tttaggggct ggaaaaacaa cgctaatcaa tcaagccttg caagtctcac
     1441 cctaccccag ggaagaaatt attataattg aaaatgagtt tggtgaagtt gggattgatc
     1501 acaaattatt agttcatagc gaggaacggg tgttacaact aaataatgga tgtatgtgtt
     1561 gcagtcttcg cagtgattta ttagcaatat tgatttcttt actggaaaca atagaagaac
     1621 atcaacaacc aatttcacaa atcatcattg aaactacagg tatagcagat ccccaaccaa
     1681 tcattcaaac acttttaaca gcgcctcaac taagaggaag agtatatatc gatagtttat
     1741 tgactgttat tgatagtgac agttttgaac ttattgaaag agaaccacaa gcccttaagc
     1801 aacttgtcat ggcagatcgc atttttgtgt ctagaaaaca aaacttgaat ttatctgcac
     1861 taaaaaaatt acagagagaa atcaaaagta ttaacccttt atccgatatt cgaatgttca
     1921 cacatagtga aacgattcaa aaaaaggatt tttataattt gaaaaaattt aatcatccct
     1981 tagtttttga aagtgattca tcagaaaatc actataatgg caaatactat catcaacacc
     2041 atcaagtaca tgagtttaaa tcaattctct taacttcaga tcaagacata aaaaaagatt
     2101 ttttactcca atggattgac tggctgattt taaccaacca agggagcctg tatcgtttta
     2161 aaggattgat tgctttacaa gagcaggatt ttatagttgc catgcaagag gtaaacgaac
     2221 aggttaattt tcaatacacc actcgacaaa aagaccaaac cactattatt ctaatcggaa
     2281 aaatgttgga tgaagaaagg atcaaacaga gttttgataa attactggcg gaaagcaaat
     2341 aatgataagt agtgattggg ggtaatttct aatggagaaa acggatatat ctagtgcata
     2401 tcgacgactt aaaagcccga atattaaaac tagaaaaagg gcattaaaaa ttatcaaaga
     2461 acataaacga aataaaatga agaaattagc ataattaagg aaggaaaaag aatgaagaaa
     2521 atcagtgtag gacttattgg gatagcagct ctaggattat tgggagcttg ctccagcact
     2581 aatgatgcaa aagtatcgaa tgataaagat ggaaaattag aaattgtaac aacattctat
     2641 ccaatgtatg atttcaccaa aaacatcgtc ggcgatgaag cgaacgtgga tttaatggtt
     2701 ccagcgggat cagaacctca tgactatgag ccctcagcta aggatatggc aaaagctcat
     2761 gatgctgatg tttttgtata tcataacgaa aacatggaat catgggtacc aaaagctaaa
     2821 gaaagttgga agaaagctgg gcctaatgta gtagaaggaa caaaagacat gattttattg
     2881 ccaggtagtg aagaagaaga tcatgatcac ggagaagaag atcaccatca tgaactagat
     2941 cctcatacgt gggtttcacc caaaatggca attaaggaag tttcaaatat caaggatcag
     3001 ttagttaaat tgtatccgaa aaaagcaaaa gtatttgaga caaatgctga aaagtatttg
     3061 actaaactga aacggttgga tgctgactat accactagtc ttaaagaagc gaaacaaaaa
     3121 agttttgtga cgcaacacgc agcctttgga tatttagctt tggattatgg attgaaacaa
     3181 gttcctattg ctggactaag tcctgaagaa gagccatctt ctggtcgatt agctgaatta
     3241 aaagagtatg ttaaaaaaaa taagatcaat tatatttact ttgaaaagaa tgctaatgat
     3301 aaaattgcaa aaactttggc caacgaagct gggataaaat tggaagtact gaatcctctt
     3361 gaaagtctta caaaagaaca aatggataat ggtgaagatt atgtttcagt aatggaagac
     3421 aatctaaaag cgttagaaaa aacaactatg gttgctggtg aagaggtagt tccagaaaaa
     3481 gaagcgaagg atgaaaagac agttgctaat gggtacttta aagatgccga tgtgaaggat
     3541 cgcgaattga gtgattatac aggcgaatgg caatctgttt atccgttgtt aaaagatgga
     3601 attttggatg aagttttcga ttacaaagca aaaatcaata aagacatgac agctgccgag
     3661 tacaaagatt attatactac tggctataag acagatattg atactatcaa cattaaagac
     3721 aacaccattg attttgtagt caacggtgaa catcatcaat acacatacaa atatgttgga
     3781 tacaaaattt tgaattatga aaaaggaaac cgtggtgttc gttttaattt tgaaactgat
     3841 gatgcaggtg ctggtcgttt taaatatatt caatttagtg atcatggaat tgcaccaagt
     3901 aaggcagagc atttccatat tttctttggt ggagaaagtc aagaaaaact ctataatgaa
     3961 ttgcataatt ggcctacttt ctatccagct tcattgtcta aacatgaaat tgctcaagaa
     4021 atgatggctc attaaaattc aatttttgga ggaaatacat ggtaagaaaa gtattattgt
     4081 ctatggattc caagaatatt gatgcttatc ggcgttatcg atttgcaaag caaatgagat
     4141 taaatgggag tacattagct gatattgctg caactttaca tgttagtatt gacacagttc
     4201 atagagtatt aaaacgggat cctgattgtt gttttatcaa ggtcaatact ggaagaagta
     4261 gacgtttagc aaaacggcat aatagcttgg ccaattaatt gataatacca gagtagatca
     4321 tttttccctt atttggattt gatctgcttt ttattatgtc ttgaattgtc aaatcaagcg
     4381 caacacttta tcaaagaaga cttcataagg tgttctccaa tctaaacact ttctaggtct
     4441 gttattcagt ttttcaatca tctgatttat cgttgagtta tctaccgtag ctatctcttt
     4501 cgattttggc aagtattctc gaatgagtct attcgtattt taatttgttc attgttgcca
     4561 agggcatgag gatctggaaa ataaaagggc atattattca atgcttctgt aactgcgaaa
     4621 tgattggaga attcttttcc tctatctgga gtgatcgttt tgaccgcctc atttggaata
     4681 ctgcttaaaa gttcaatcat tttctcgctc acataagcgg ctttctttct ggggaccttc
     4741 tttcctgcta aaaggaagca tgattttctg tctgtcaaag tcactaaaca agtaccacca
     4801 gttttgccag ctacaatatc ggctttccaa tgaccaaaat cacttctatc atctgcggat
     4861 tttggacggc catggatcga atgagtaagg caaatctttc cacgagactc gacatgattt
     4921 tttgtatgtc ttgtttttcc tcgatggtgc aatgaacgga tcaggccttt attgccgtgt
     4981 gagaggggtt ctgtatcgaa aagatcattg caaattgcac gttagatcgt gataaaacta
     5041 attgaaaagt ccatatgttc atgcttcaaa cgattcgaga tttcctctgg cgaccactga
     5101 aaatcgacaa ataaatgacg aacaattgac caaactcacg gatcatttag aatgtgataa
     5161 cgtccgcatt tttttcttcg ataagcataa tttttttccg ctttggaagg tgaatagttt
     5221 ttttcttgtt tcttctcagt tcatgtaaaa tagtaaacgg gctatgctga atgtcgttgc
     5281 caattatact aatagaaaat ccttcttcgt gaagcagaaa gattctttct cgttcgagta
     5341 tagtaagatg acgatactgg ctcataatta ttttccttct aggagtgttc tcgacaaact
     5401 tatactagtt ggatatcgat tatgactcat ttttatgtgt tgcacttaaa gtgtaaattc
     5461 aaggtataga aagaggttcc ctatgaaaaa aatacctatt acaatcttaa ctggctatct
     5521 tggttcagga aaaacgatac ttctaaatca tattctgtac ggtgatcacg gaaagaaaat
     5581 tgctgtcatt gtaaatgaca taggggaagt aaatgttgat ggggtattag tcgacaagaa
     5641 aggttttaaa agaactggag aaaaacttgt ttcgatgccg aatggatgca tttgttgtac
     5701 attacgcgaa gatttgattg ttgaactaga tcaattagtg caattttctg acttgaatca
     5761 aattgttatt gaagcttcag gaatcagcaa accaattcca attattcaag caattatatt
     5821 agctaaaaac cctttagaca ttgatttgac ccaacgatta gagattggtg ccattattat
     5881 agtagtggat gtaaaacgtt tttgggataa tttcgaaacg ggtgaaaaga ttcaggaaag
     5941 aaaagctgaa ggtaatgtag ttgaagaaga acaggatatt cgtggcttgt taattgatca
     6001 attcgagtgt tgtaacatat tacttttaaa taatagtgac ttagtgtcaa ccaagacgat
     6061 ttacaaaatt aacggtctaa ttgctaagtt acagtccaat gccaaaataa ttaggacaat
     6121 taaaggtgaa gtagacttat cgaaaattct aaatacaaat ctttttaata tagatgagac
     6181 ttcctattca gtgggctggt tcaaagagtt agagcttggc tatcaaaatc atattcctga
     6241 aacagaagaa tatggtatct caagttttat ctatcgaaat cggttactcc ttgacgcaca
     6301 aaaatttgcc catcttacgg ataaaaattt tcctggcaat attacaagag caaaagggtt
     6361 tctttggttt gcccaacata gtgatttttc gacgatcttt gaaacatcgg gtagctataa
     6421 aagtattgaa tcattaaatt attgggtagc tagtttacct aaaattgagt gtcaaatgat
     6481 tatacaaaaa aatcccgaat tgaaggaagc ctggcatccg ttgtttggtg aacgcattaa
     6541 taaactagtt tttattggta tgaagatgga tcaacaatgt attaaggaac aacttgaggg
     6601 ttgtttaact gattctctta atgcagataa tttccgaaaa atttcaccat ttccatggaa
     6661 aaagtctaat atgtttagcc agatatcgtt aatgaaattt ttaagaaaag ctggtgtcaa
     6721 gataggcgat gtcataatta ttggcatttt gattattacc tcttttattc ccttatttgt
     6781 attctctata gaaaaggaga atgaaacaac tgaagatgct atcaaatatg ccatcgttcg
     6841 aattgatggc aaggaaatgg atcgcttcga tttggataag atcaatcata aactcgtgaa
     6901 ttatcatcct gcgaagggac aatataatat tgttgagatt aaagatggaa gaattcgagt
     6961 taaagaagat aatagtcctg atcaaatcgc ggttagaact ggatggatct caaaacccgg
     7021 tcaaacgagt atttgtttac cccataagtt agttattagt attgagaaaa aagaaagcac
     7081 agattactac atttactaat ctggaaatag atgaggaggt ttttaataag aaaaagatat
     7141 tattattaat aatagcaggg gctttattaa gtgtgttaag cgcttgtgga caagaaaaag
     7201 aagcggtgac aacaggcaca agtatcttta acgggacctg caatggttta gttaacagtc
     7261 ggttagatta cttattttgg ctcttcgtca atgagtgttg gtgaagaaat tcaatagaat
     7321 aatcgagata ctcacacggc tagacagcta tcttgtctag ccgtgttgtt gttctggctt
     7381 tagtcagttg ggtagtattc ttagttttct taaaatattt tcctcgatat aaaaagattc
     7441 gttctcggta gttttgaaag tttctatagc cataggcaat tcgttttagt actttgatat
     7501 ggttattcaa acattctata gggccgttgg aatagggaac gcgcaacgct cgtttgattt
     7561 ctgtttggta tttcttgaag gtcgtgaaca ctggttggta gtgctctggc aactccttgt
     7621 agtcttcttt taggagcgcg acgaattcag gaacatcttg tcttctgata acggaaagaa
     7681 aatcctggta gacttcgtag cctcgcttta aggaatcatc ataagataat agccgatcga
     7741 ctatctctgc ttctgtcaga tgatctcgaa atgacggacg ccagtagcga tgctgacctt
     7801 tcaattccca cgcattcttt tgaagtagtt tccagtattt ctttaagtgc ttcgaccgac
     7861 gatccccttt tttaaaggtc ttcattactt gaattcggtg gttggtaaaa gcccgaccaa
     7921 tgtgttggct aatatgaaag cgatcggcga cgattcgggc attcggaaaa cactctttca
     7981 ccaatgtgcg atagggtgta tagaaatctg aaaccactag acgcacttgt tcacggactt
     8041 ttatagggta gcgcaagaaa tagtcgcgta aaggattgag ttgccgattt tccacaatat
     8101 caatcagttg atgggtcttg ccgtccatca tgacaaagct catggctccc gagacatttt
     8161 tcactgattt gaattcatcg aaacacacca cttctggtaa cagctggctc aaagaaatgg
     8221 ttttaggccg aaggctgcga agtattcgaa tgacagtggt cggagaaatg tgcttcatac
     8281 gtgcgatcaa agacatagaa agaggttcgc ttaatagctc aagaatggct tgtttgactc
     8341 tgcgagcaat ggagcagcga cgatcactaa ctgaatcttc tgctaaaaaa gtctttccac
     8401 agtgtttaca tttgaagcgc tgtttgttaa tccgcagaat ggtcttgtag ttactgacat
     8461 cattcaataa gatggatact tttttccagc cctatttaat gatcgtttgt tcattttgat
     8521 agtggcgatg aaagcattcc tccggttgat aggtcaatgt gccatcaaaa acaaaacatg
     8581 tttcgtcatt gattcgctct ttgcgaaagg catcttcatg aaagataaga tttaggtcta
     8641 gaatatctag taactctttg gtaaactctg aataggacat cttgaaacac tcctttgaat
     8701 ggttttcgtc gactttattc taaaggattt taaggtgtct gtttgcgttt attagaaaaa
     8761 agaaaaagta ttggcaacag aaggcgtcac caacactaga tattatagat cccttatttt
     8821 cttcgcttca aattgtcaag gagtcatcat aaattttgta tggattcttt tgtttgggta
     8881 gtagtcttct aaataatctt ggattaacac cttcatctct attttacttt tatatttgtt
     8941 ggagtcgatt aattctcatt tgattgatcg atgaaacgct tcaattactg cattgtcaac
     9001 aggagtccca gtttggctca ctgaatgtgt gatgtggtgg tcgttcaaca gctcttgata
     9061 aataaaagac cgatgctggc tacctctatc ggaatgaatt accgtaggca attctcctta
     9121 ataaatttgt aatggacgga cgacaatctc ttttttcatg tgtgaatcta ccatatagta
     9181 acgtagaata cgagttgcta agtcaatata ggtggacaaa tagaggcgac cgtcattaca
     9241 tggaatgtaa gtaatatcca tcacccaaac agcatcaatt tggttctgtt caaattatcg
     9301 attcaacatt tttttaccat ttctttgatt gttttttctc taccatgaga agatttgcgt
     9361 cgatgaaatc ctcttgtgca taaattcatt tcacgcataa gaacagctac aacacgttta
     9421 ttgatttcga ttccttcatc gtaaagtttt tgagtgatcc ctggagatcc gtatcgatct
     9481 ttgtgttcgt agaacactcg tcttcctctt ttagtcgttt attttcctgt tcaagaatgt
     9541 agaaatctgc tttatcaggt aatctttttc cttgtccagc aaaagcattt ttcccgtatc
     9601 gacgatattc tgaaacccat cgggaaattg ttggacgaga gatattgtat tcttcagcaa
     9661 ttttcttggt agttactttt ttctcaagga tatcctgtga aatctttagt ttaaatttac
     9721 gatcatatgt ttttctcaat agagttcatt ccttccggta acgtaactga tagtcaagaa
     9781 aagtgtatca tagcccaact tagtatcagg gtttgtcaac taaactgtgg aagttgatag
     9841 ataagagttt ttaacaacca aaattctctt gactattttt gaaatcagac aaattttgat
     9901 tggacacata gttgaataaa tctaccgtcg ttacggaagg taaatcgcat gcttttcaat
     9961 tctagaggag aagtcctcgt tgaagtacca tagcccagtt ctggattcga cctcctgccc
    10021 acttcatacg taaataaagt aattttagga gtgagactgt gacaaaagtt atttttaagc
    10081 taaaaacgaa ttaaaaaacg ttgataatta aaatcattcg ttttagattc tatgaatcat
    10141 tatagaacgc tgtaaaatcg atactttata attataaaga agatagtttc aaatttattg
    10201 ttcgaaattt gaaactatct ttttacttat gtcacagact cccttagttg agttattctt
    10261 taatttcgaa tttcgagata gctagatata ttgcaagcat acattcttga taacaatctt
    10321 gatcatattt tccagttctc caggattgct tatgaatcaa agagctaaat cttttgagaa
    10381 ttgcctcaac agcttcttca tttccagcct ttgctaaaat gaaatcttct tttaaagaat
    10441 tcataaatct accttcttcc catggactta taatctattt ctttgggaag gaggagaacg
    10501 agcaaagaag catacaaaat tagttattat gcagatatta aaccagcatt ttttgaattt
    10561 ttttgtaaag tcgatgtttt aaatttgtcg caccctgtct tgtaatcccc agaacctttc
    10621 caatttcttt atcagtttta tcaaagataa atttttcgat aagaaaaatt tgttcaggtt
    10681 ctttcaacaa aggaaggatt tttgctaatt ttatatcttc aacaaggaga gtcacattta
    10741 aaacattaat cggttgttta aattttatag tatctttagt aaacaaatat ggtgggagtt
    10801 gatcaagttg ttcatgtttt tgaatttctt cttgtttttt ataataagta ttagcggtgt
    10861 tcaaaatgac cttttcaaag aatcgaatca actgggcatt tacgcgttcg tcgttatctg
    10921 acatattgca cacctcctcc attaagattt aaagggaaaa gaggcaaaaa acacgaaaag
    10981 aagctaatgc aattatgctg cgctgcgaat aacttctata agctcacatg cattgatagg
    11041 actgctgatg tttattttca aaaatgtttt tttagttgat aatggatagt tatctttata
    11101 cttagaaaag aaaattcata tatcttctta aatatcaaac atgtccttat ggctaagaaa
    11161 atgtcttgag ctttttacgt ataataaatc ttttctaaaa cctccttttg gataatattc
    11221 ttattcgtgt atagattgta ttttttattt cttccccctt cataattaac aactagaaaa
    11281 gagttcgaat agacacgtat ttgaacgaaa atttttattg gattcccaaa ttggaatatt
    11341 tagaaacgaa aaattcctat tatctgtata gtgcttagat aataacggat cataataatt
    11401 attgtctttg aactagatat atgtaaatag tattcatcat gatgaaaaat gatgagttct
    11461 cggtgataaa atgtactttt tacggtggaa tatacatgaa agcgataagt ggatatttta
    11521 taatgaagta agttagctta ttttgacaga ggaatagtat tttagataac gtataaaata
    11581 ctatttaatg atgtagaaag gaaagagaga aacaaaagtg ttttcagata aagaagtctt
    11641 attaaagtct cttcatgttt gtttaaagtt acctatcgtg gtttttaatt ccgaattcag
    11701 tattataaaa gagtatcgct cagatagaac tactt
//
LOCUS       DAYYCW010000087        11616 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000087 DAYYCW010000000
VERSION     DAYYCW010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.089"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHAYA_002191"
     CDS             6..305
                     /locus_tag="ACHAYA_002191"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8605976.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHAYA_002192"
     CDS             378..1739
                     /locus_tag="ACHAYA_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM8605977.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHAYA_002193"
     CDS             1761..2021
                     /locus_tag="ACHAYA_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605978.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHAYA_002194"
     CDS             2035..2787
                     /locus_tag="ACHAYA_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM8605979.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGKKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHAYA_002195"
     CDS             complement(2881..3486)
                     /locus_tag="ACHAYA_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8605980.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHAYA_002196"
     CDS             complement(3539..4513)
                     /locus_tag="ACHAYA_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM8605981.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHAYA_002197"
     CDS             complement(4891..5610)
                     /locus_tag="ACHAYA_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8605982.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHAYA_002198"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHAYA_002198"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8605983.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHAYA_002199"
     CDS             7161..8558
                     /locus_tag="ACHAYA_002199"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8605984.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHAYA_002200"
     CDS             8639..9079
                     /locus_tag="ACHAYA_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8605985.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHAYA_002201"
     CDS             9227..9553
                     /locus_tag="ACHAYA_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8605986.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHAYA_002202"
     CDS             9554..10303
                     /locus_tag="ACHAYA_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM8605987.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHAYA_002203"
     CDS             complement(10383..10838)
                     /locus_tag="ACHAYA_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8605988.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACHAYA_002204"
     CDS             complement(11265..11540)
                     /locus_tag="ACHAYA_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8605989.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3669 a         2015 c         2360 g         3572 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtaaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactga
//
LOCUS       DAYYCW010000088        11511 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000088 DAYYCW010000000
VERSION     DAYYCW010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.090"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..1063)
                     /locus_tag="ACHAYA_002205"
     CDS             complement(14..1063)
                     /locus_tag="ACHAYA_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8605990.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSVVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFTRGAS"
     gene            complement(1145..1834)
                     /locus_tag="ACHAYA_002206"
     CDS             complement(1145..1834)
                     /locus_tag="ACHAYA_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8605991.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1940..2308)
                     /gene="rplL"
                     /locus_tag="ACHAYA_002207"
     CDS             complement(1940..2308)
                     /gene="rplL"
                     /locus_tag="ACHAYA_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM8605992.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2361..2864)
                     /gene="rplJ"
                     /locus_tag="ACHAYA_002208"
     CDS             complement(2361..2864)
                     /gene="rplJ"
                     /locus_tag="ACHAYA_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM8605993.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3068..3195)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3274..3963)
                     /gene="rplA"
                     /locus_tag="ACHAYA_002209"
     CDS             complement(3274..3963)
                     /gene="rplA"
                     /locus_tag="ACHAYA_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM8605994.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4067..4489)
                     /gene="rplK"
                     /locus_tag="ACHAYA_002210"
     CDS             complement(4067..4489)
                     /gene="rplK"
                     /locus_tag="ACHAYA_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM8605995.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4832..5500
                     /gene="sdaAB"
                     /locus_tag="ACHAYA_002211"
     CDS             4832..5500
                     /gene="sdaAB"
                     /locus_tag="ACHAYA_002211"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM8605996.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5513..6385
                     /gene="sdaAA"
                     /locus_tag="ACHAYA_002212"
     CDS             5513..6385
                     /gene="sdaAA"
                     /locus_tag="ACHAYA_002212"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM8605997.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6476..7576)
                     /locus_tag="ACHAYA_002213"
                     /pseudo
     CDS             complement(6476..7576)
                     /locus_tag="ACHAYA_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7668..8555)
                     /locus_tag="ACHAYA_002214"
     CDS             complement(7668..8555)
                     /locus_tag="ACHAYA_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8605998.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8632..9177)
                     /gene="nusG"
                     /locus_tag="ACHAYA_002215"
     CDS             complement(8632..9177)
                     /gene="nusG"
                     /locus_tag="ACHAYA_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM8605999.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9278..9448)
                     /gene="secE"
                     /locus_tag="ACHAYA_002216"
     CDS             complement(9278..9448)
                     /gene="secE"
                     /locus_tag="ACHAYA_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM8606000.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9472..9624)
                     /gene="rpmG"
                     /locus_tag="ACHAYA_002217"
     CDS             complement(9472..9624)
                     /gene="rpmG"
                     /locus_tag="ACHAYA_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8606001.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9786..10430
                     /gene="cbpA"
                     /locus_tag="ACHAYA_002218"
     CDS             9786..10430
                     /gene="cbpA"
                     /locus_tag="ACHAYA_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM8606002.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10486..10989)
                     /locus_tag="ACHAYA_002219"
     CDS             complement(10486..10989)
                     /locus_tag="ACHAYA_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8606003.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(11002..11048)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         3366 a         2386 c         2098 g         3661 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg taaatacagt attatttcgg ttgcttgctg
       61 cttcacctag cccttgtact cgtgtttgat tggcgacttg accaaattga ttcccgacgt
      121 ctaataaaat aatagctaaa agcaaaatgg ccaaatggct tccgccaacc aataaagcaa
      181 taaagcttat tgtttgcata acaagtccca tatacacgat ttttctttca gaataagaat
      241 ctgccagtcg tcccacgata ggtgcagcaa ctgctccaga caaaccaaaa atcgctaata
      301 tccctgcttc gaatgttccc caattgtagg cgggactgct gatataaaaa atcaacgtag
      361 accaaaagat cgaaaatgtt ccaaacatga aaaatccact gactgccgct tctcttaata
      421 gacgttgaga agcaactaaa tgcggcaaac ttttaagtga atcaaggtat gaaacagctg
      481 tactactttg ctggacagaa cgcggcattt tccagtaaag cagaagtgta agaattccga
      541 ctgcacataa agcaaataaa tagatcatcc gccaagaaaa aagactggca acaataccag
      601 ataccgtccg agaaagcaaa atccccacga gtaaaccgct aagcaatgta cccatgacct
      661 tccctcttgc ttttggtccc gctagtaccg caccgtaagg aatgatgatt tgcggcacga
      721 tcgaaagtaa tcctattaaa aaagaagcgc atgcaaataa cgtaaacgaa ggcgcaaaaa
      781 aagcggccaa cagagagacg gcagataacg ctaccattcg aatgatcaat tttggacgat
      841 ccaccacgtc ccctaaagga actaaaaaca gtaaccctaa cgcatagcct aattgggtca
      901 acatcgtcaa tatcccgact acacttgtgc taacagataa actagctgct acttttgttc
      961 caatcggctg gatgtaatac atattggcaa caacaacgcc acaagtgatt gataatagta
     1021 aaataagcga agttgttaaa ggtgtttttt cattactctt cattgatttc ccccatttcc
     1081 tatcttagtt tacaacttat ggaaaagatt ggaaatagaa aaatacaact attcgccaca
     1141 cttattattt gtgcttttgt tttccttgtt ttttcttgcg atctttataa atgaagacaa
     1201 ctaaccctac tagcacagta acacttgcaa ccaacgtgac tgcagttttg ttatatccgt
     1261 gaaacagagc ttctccacca cacgcataca acacagacga agggatgact ccaatcacga
     1321 tcgacaatat gatttgctgt agaggcaatt tcatcgcatc tgcagcaaaa ttgaccatag
     1381 aagatggaat aaccggtatc atatagccta acattacacc tatttttgga tgtttcattt
     1441 gtcggattgc ttgcacccaa tgattcgttt cggttttctt atctaaaaat tttaagcgat
     1501 gcataaggaa aattgcagtg atgttaccaa gagcattacc caaaatgttg atgatcgcac
     1561 ctgtcagtgg tccaaagctc aaaccagcaa tgactccaac tactgaagta ggaatacctg
     1621 gaacagcaca catcaccgct gtcaatacga tcaagagtat cgatgcaaaa gtcccatgcg
     1681 aacgaatatg tgcgagtagc tctgccttac tcgctctcgg atttaaaaat aattggatgt
     1741 ctgtctgata ttctaaatac aaccgatagc ccactaccag tatcagaatg attcccaacg
     1801 agatcaacat caatcggatt attttctttt tcatatttcc tcctgaaaaa aatctatatt
     1861 cattaaataa tagtttaaca taaaaacaaa aaagcaccga aatccaacat aggatctcag
     1921 tgcttttaaa tagtaaaaat tatttaacag ttacagatgc gccaacttct tcaagtttag
     1981 ctttcaattc ttcagcttct tctttagata cgccttcttt aactggtgca ggagcgccat
     2041 caactacagc tttagcttct ttcaagccta aaccagttgc ttcacgaact gctttgataa
     2101 ctttaacttt ttggtctcca gctgcagtta attctacagt gaattctgtt tgttcttcag
     2161 cagcagcagc gccaccagca gcagctacag ctacaggagc agcagcagat acgccaaatt
     2221 cttcttcaat agctttaact aggtcgttaa gttctaggat agttgcttct tttaactcag
     2281 caacgatgtt ttcaatgttc aatgccatta taatttcctc cgtttaattg ttctttttta
     2341 tttaatgctt tatgcggcaa ttaagctgca tctccatctt ctttgtctgc cactgctttg
     2401 acagcgtaag ccacgttgcg gactggcgct tgtagtacag atagcaacat agatagaagt
     2461 ccttcgcgat ttggcaattt tgccaatgct gtgatttctt ccaatgaaga gactttacct
     2521 tcgatgatac cgcctttgat ttctaatgct tttgcttctt tagcaaattc gtccatgatt
     2581 ttcgctggtg cgactacatc ttcgttactg aatgcaacag ctgtaggtcc agtgaatact
     2641 tcgtccatgc cttcaaggcc agctttttta gctgcacgtg aaagaataga atttttgata
     2701 actttcattt ctacgttagc atcacgaagt tgtttacgta aacgtgttac ttcatcaact
     2761 gttaatccgc ggtagtcaac gactactact gatgcagctg ctgtaaattt ttcgcttact
     2821 tcatcaacga ttgctgcttt ttttgcgata attgcttcac tcatttttga gtttcacctc
     2881 ctgaattttg atggaagagt tttttgcact gaaaaggcaa gtcactttta tagttacttt
     2941 gctcaaccag tattattcat cacctattca cttcgttcat agtgattcta ctcgctttgc
     3001 tcgaaaggaa actcctttcg ctttgctcaa ccagtattgt tcatcatctc ttcacttcgt
     3061 tcatagtgat tcacaataaa aaactctatg ccaccgcaga catagaggga ctattgaata
     3121 tattcaataa acgtcctcgg taggaaatta aggcttgatg ccacctactg tcttcggtac
     3181 aagttaatta ttaacctcaa ctagcttagc atgctaaact agttgagtca agaattattt
     3241 ttcagttgga tcaatcaaca ttctaatatt taattagaaa gaagcttggt caacatggat
     3301 accaggtcca aatgttgttg tgatcgttac atttttcatg tattgacctt tagctgctga
     3361 tggtttagct tttagcaata catcatggat tgctgcaaag ttttcaacta atttttcatc
     3421 gtcaaatgaa acttttccga ttggtacatg gatatttcca gctttgtcca cacggtaagt
     3481 tactttacca gctttcactt cgtttactgc ttttgttacg tccattgtaa ctgtaccagt
     3541 tttagggttt ggcattaagc cttttggtcc taatactcgt cctagacgac ctacagtagc
     3601 catcatatca ggtgttgcaa caactacatc aaagtcaaac catccgtttt ggattttttg
     3661 aaccatgtcg tcgtcaccta cgaaatctgc accagctgct tcagcttctt ttgctttgtc
     3721 tcctttagca aaaactaaaa cagtttgtgt tttaccagtt ccgtttggta atacaacagc
     3781 accacggatt tgttggtctg cttttttagg gtctacgttc aaacggtatg caacttcaac
     3841 tgttgcatca aattttgcaa tgtttgtttc ttttgctaaa gctactgctt cggcaacagg
     3901 ataaacttta cttgaatcaa ctttttttaa tgcatcttgc atttttttgc tctttttagc
     3961 cattttggtt cctccttgat tgtggttata acggttgtac ctcccacgtg ttctatatct
     4021 ttcaatatag agccaactga gaagctgctg tcttagggtg tatagattat tctacagtga
     4081 tccccatgct tcgtgcagtt ccttcaacca tgcgcattgc tgcttcaacg ttagctgcgt
     4141 ttaggtcttc catttttaat tcagcgattt ctcttacttg atcgctagaa actttcgcaa
     4201 ctttattttt gtttggttcg cctgaacctt tttcgatttt agctgctttt ttcaatagaa
     4261 cagcagctgg cggtgttttt gtaacgaatg tgaatgaacg gtcttcgtat acagaaataa
     4321 caaccggaat gatcaaacct gcttgatcag ctgtacgagc gttgaattct tttgtgaatc
     4381 ccatgatatt gatacccgct tgacctagtg caggacctac tggtggagct ggagttgctt
     4441 tacctgcagg aatttgcaat ttaacgattt tttctacttt ctttgccacg agacatacct
     4501 ccttaagtcc gtgatgtggt taattggaga tgcatatttc tcctcccacg ttttatctga
     4561 tctgaaaaaa tccagacaaa taatgtgcaa aacaatgcac actggtacag tatagcaaat
     4621 ttatttgttt tttcaagatt tttttctgct atttttacat tttgcttgaa taaacgctaa
     4681 atttattcta aaaaaacaac aatttttcgt tctcatttct tcttttatgt tacacttgcg
     4741 ctagcatggt tcaacagatg aaagcaaaca ataacacggt tttttagacc cttgtacaaa
     4801 gattcctaca taacaaaatg aggtggagaa aatgaaagat ttaaggtata gaagtgtctt
     4861 tgatattatt ggacctgtga tgattggtcc aagtagctct cacactgccg gagctgctag
     4921 aatcgggaaa atcatacgta gtatatttgg cgaacagcct gattctgtag atatttatct
     4981 atatgaatca tttgcaaaaa cctatcgggg gcacggaaca gatattgcat tagttggcgg
     5041 cctgttaggg atggaacccg atgatgaacg tttagctgat tctttgaaat tggcatatga
     5101 agccggtatg gaagtcttgt ttgttcctaa gagtgaaaaa gccgaacatc caaactctgt
     5161 aaagatgctt ctaaaaaaag gaacacgaaa gctttcagtc actggcattt cgatcggcgg
     5221 tggcaatatc cagatttctg aattgaatgg attcaaaatt tctttaagca tgggtactcc
     5281 taccttgatc gttgtccacc aagatgttcc aggtatgatt gctaaagtga ctagtatact
     5341 ttctgagacc aatacgaata tcggcactat gacagtgaca cgggaatcaa aaggtgaaaa
     5401 tgccattatg ataattgaag tagatgatcc tcaagtagaa gaaacggtaa aaagactcaa
     5461 acagctgcca aatatcgaca gcgtaaatta tttcgattaa ggagctaaaa aaatgttttt
     5521 atcgattgaa gaattagtag atcaagcaaa agcttatcca agtgtagcag aattgatgat
     5581 tgcagttgaa atggaaatga gtggccgttc ccgtagtcac atcatcgaga tcatggaaaa
     5641 aaacctctca gtaatggaac aatcaatcaa cgagggaaaa gaaggtgtta cctccgtcac
     5701 aggaattacc ggtggtgatg caaaaaaatt agatgattat ttaaatgagg cagacttttt
     5761 aagtggagaa acaatcttaa aagctgtacg caatgctgta gctgttaatg aagtcaatgc
     5821 aaaaatgggg ttgatttgtg ctacaccgac tgccggaagc gcgggtgttg ttcctggtgt
     5881 gctgatggct gtccgagatc ggcttgattt gacacatgaa cagcaattgg aattcttatt
     5941 taccgctggc gctttcgggt tagtcatcgc aaacaacgct tctatcagtg gtgcagaagg
     6001 gggatgtcaa gcagaagttg gttctgcaag tgcaatggca gcagcagctt tggtttgtgc
     6061 aaaaaatggg actcctcatc aagcagcaca agcagtagcc attacattga aaaatatgat
     6121 gggactgatt tgcgatcccg ttgcaggctt agttgaagtc ccgtgtgtca aacgtaatgc
     6181 tttaggctct tctcaagctt ttatctctgc tgatatggca ttagcgggta tcgaaagtgt
     6241 cattccgccg gatgaagtcg ttgctgcaat gtaccaagtc ggacgtcaaa tgccgtcgat
     6301 tttcaaagaa acagcagaag gtggcttagc tgttacgcca actgctaaac gacttgcgga
     6361 agagatttta aataagaggg cgtgataaag ctccttaatt tcgagtatta agagattagt
     6421 tatcttcata acaaaaaacg aagagaggac taattgccct tctcttcgtt ttttattaat
     6481 ccatcaattt cgtatttttt acttcatcga tcgtttttgt tcctgcaagt tgcatcgtga
     6541 tcgacaattc tttgttcagg tgttcaaaaa cagatgttac tccttctgcc ccacctagat
     6601 tcaacccata aatcactggt cggccaatcg caactaagtc agcaccactt gccagagctt
     6661 tgaagacatg ctctcctcga cggacaccag agtcgaaaat cactggtaca cgtttgttga
     6721 ctacttcggc aatttttggc aatacttcaa aggacgcagg acctccatct agttgacgtc
     6781 caccatgatt ggatacccag ataccatctg cccctgcaga gatagcgatt actgcatctt
     6841 ctggcgattg tattcctttt acgataactg gaagatgggt gatttctttg attgttttga
     6901 tgtcatctgg tgtcaagcct tgttttgcag ctgcataaat ttctgcgatt ccttttcctt
     6961 cgccattacc acttgcactt tgttcgctat acgcagctaa gttaggcatt ggtaatggaa
     7021 attggaattg attgatgacg tcttcttcac ggtaaccacc gagcgttgaa tctgccgtta
     7081 aaatgatagc tttagcccct gcttctactg ctttgttcaa aatgaactca ttgaatctgt
     7141 cgtctttact catatataat tggaaaaact gcggtgcgcc tggcgctgca tcggctgcgt
     7201 ctttgattgt cgtatttgca taagtgctga tggaaaagat tgatccagca gcagctactc
     7261 ctttagctgt atcagcttcc cctttttcat gtgccaatcc ttgggccgct gatggcgctt
     7321 ggataatcgg tgtatctaat tcaattccga aaacagatgt atgcaaatcg gctgagtcga
     7381 ttccgcgtaa tatccgtggc atgattttct ttgtattaaa agaagtcgta ttttctttca
     7441 tcgtccactc atcttcagaa ccaccgcgaa tatagccaaa agctcctgct tccatgcgct
     7501 cttttacacg accttctagt taggcaatgt ttacaatatc gattggatgt tcttcattac
     7561 ttgtatgata agtcatagta gatccttctt tcatcttctt gattacatat aaatcatact
     7621 aaacttacaa aaagtaagca aacatttact ctcagatact tttatcgcta ttctttccaa
     7681 ataaatcgac ttacaagctg atctacttct tggttttcat gaagcatact atgagaggca
     7741 ttttcacctg tcactacttt ttcctctatt tcatttccgc gttgcttcaa caacgcatag
     7801 accgctaatg cactgtttaa cggtactgtt ccatcactca agtctgtttc atctagttga
     7861 ccagcaatca agaaaaatcg agtggttata ggaatattac cgatcatttg ctggtaatct
     7921 tgatagcggt tagaaacgac agcaggcccc tttgctaact catctgtcag agactgcgca
     7981 ctatcatcga caaaatcatt aaaaggcgcg ccaattgctg ttaatgtctg aacttgtggc
     8041 aatgacgtat cctgtccata cgttcctaaa tagcgtagtg cactaactcc tcccatggaa
     8101 tgccccacta tgttgacttt atctatgccg tagttttctt tcagatacaa aagacaagtt
     8161 ttgatccatt ctgcttgatt ccactcgtta tttttattat cttcaaacaa tacttggacc
     8221 atcggattgt ctgcttttcc agacaaatct cctgttgctt gtatttgtcc atctgttcct
     8281 actgttaaaa ccaattcttt tactgcttgc tgattttttt ccaaacgccg caacatccct
     8341 ttaaaggaat tttctgtccc gctgtagccg tggaaaaaaa gtgttggaat agtggttttt
     8401 tctatcactt tactggattc tgccgttttt tctttatcag aaacttcagt agtatttatc
     8461 ttcgtcgtct ggcaaccact taacgcaaac aaactgataa ggactaaagc tgctgcagtt
     8521 gcacgtttgc ataaattttt gattggttct gtcatacacc tcttcctttc catatagaaa
     8581 aaagaaagac ttgaaaggcc aatcatatga tcgacatttc aagtctcttt attaatcgat
     8641 tttatctact tgatcgaaat caagttctgt actggtttca cgtccaaaca tatcgatgtt
     8701 gactttcaat ttttcttttt ctccgtcaac ttctgttact tgtccttcta atccagcaaa
     8761 tgcgccttcg atgattttca cgacttcgcc aacttctact tctagatcat tttgtcgcag
     8821 gctcataccg atagaacgca agatatgatt gacttcttct ggaagtaatg gtgctggttt
     8881 gcttccggct ccgtgtgaac caacgaatcc tgtcacacca ggtgtgttgc ggacaacata
     8941 ccatgaagcg tctgtcatga tcatttcaac aagtacgtat cctgggaatg ttttatgaac
     9001 gatttctttt tctttcccat ttttgacttc agtttctttt tcttcaggaa caacgacacg
     9061 gaaaataaag tcggccattt tcatactttg cgcacgagat tcgatatttg cttttacttt
     9121 gttttcatat cctgaatatg tatgcaagac ataccagttt ttttcaaaag attccattga
     9181 aggctcctca tttcattgta aaataaaaaa acctcagtac acccgaagtt tttctttcgc
     9241 ttcattatag cactaaaagt acgttgtggc tagcgagtta ttttaaaatc cagccaaata
     9301 agctttggat ggctgtatcc attacgtaaa acatcaatcc gaaaataata gaagtttcga
     9361 tgactactaa cgtatcttta cggagttgtt ttctgcttgg ccaagttact tgtttgattt
     9421 catcttttac gctttttaga aatttcatga gacaacctcc cttgctactg tttatttcgt
     9481 ttctttatgt aatgtatatt gattacaata tttacaaaac ttattgattt ccaaacgttc
     9541 gccgcttttt ccttcactga ctgattttgt gtagttgcga gacccacaaa cggaacaagc
     9601 gagtgctgct tttttctttg ccatttactt ctgccccctt gctgagaatc agacttatac
     9661 ttcataacag tatcacgtaa ctttttactt gtcaatttgt gaaataaact atctattgtt
     9721 ttggataaaa ttatgatatg attaatttga aattaagcta aaatgagaca attggaggta
     9781 tagcgatgct attaaaaaca cttgtatata agaaaaaaga tctgacaacc gttacagaga
     9841 ctagtacatt agaagaagca ttaaaaattt tagaaacttc cggataccgt tgtgtgccga
     9901 tcttagattc ttcaggtaat attttccgtg gaaacattta caaaatgcat atctatcgtc
     9961 ataaagcaaa tggcggtgat atgacgcttc ctgtcactca tcttttgaaa aacgctacaa
    10021 agttcattta cctggatact tctttcttca aagtattctt tacgatcaaa gagcttcctt
    10081 atattgctgt ccttgacagt gaaaatcatt tttatggtat tttgacacat agtacgttgc
    10141 ttaatatgct ctctcaatct tggagcgtag aacaaggaag ctatgtactg acaattgctt
    10201 ccacaggaaa gcaaggagat ttggctgcta ttgcaaaaat catttcaaaa cacagcagca
    10261 ttgccagctg tattactctt gatatcgaaa aagacgaata cattcgacgt acattgatca
    10321 cgctaccggc taacactgaa gcgaaagtat gtgaaacgat cgtcaaccac ttagagaaaa
    10381 agaattataa agtggtagaa attgaaaatt tgcaaaacca agaagcataa tcaaaaagcg
    10441 aaaaaagaaa acagctaatc tgctttcttt tttcgctttt tataattaga cacgcttttc
    10501 aaaatcaagc gcgcgatcta aataatacat caatggtgct gaaacagcca tgacgataaa
    10561 ctcacttaat gcaagcgtgc cataggttgt ccaaaatgct tggccacctg ttggaactaa
    10621 catataagca atcagtccca tgctgatggt aaagaagatc gtgttcaata ctaaacgcat
    10681 ctttacattt ttcactttgt tttgcaataa agctgtcagg cctaaagaaa gcaatgtttg
    10741 gccgccacca aaaaccacat ccatgatacc aaaaccaaag aaggcattat agacgatgac
    10801 cccgcctaaa actccccaaa gcaatttacg gttaaagaca actaaatgat tcaaactttc
    10861 tgaaatacga aactggatcg gccctgagga tacaggcgca actaaaatag ttaacgctag
    10921 ataaagagcc atgatgatgc catttacaac gattacggat aaccgttgtc tgcttttcgt
    10981 tttttgcatt ttctctcctc ttttctccta gtttttttaa cgcgggatgg ttgatgaacc
    11041 gcggttatgt gaaacaaaca ataattataa cagacttttc atttatttca atccgtctta
    11101 tctgctttta aaaaagcaaa agcttaaaca atgtaaacgt ttgctaaaaa tcgtgggata
    11161 tggtataaag taagtgtaag gggtcataag aattcccagt aaagcagacg aagtaggatc
    11221 tgcaagaaca gaattgttgt gaaacttaaa taaataaaaa aggatgtggg aatttgagca
    11281 acctcttact agagaacggt ctagcaagca gctgaagatc taagtggaca tgccgaacct
    11341 tggaacttct ccttacgtaa gtaatggatc atgcgcttgc taggcttttt tgtgtttttc
    11401 ggtcttcgga cctttttcat tttcctagac ttctgctata ataaccaaga attaattttg
    11461 taacagattt ttttgaaaaa acaagtttct ttcattcaaa aaatagaaaa a
//
LOCUS       DAYYCW010000089        11423 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000089 DAYYCW010000000
VERSION     DAYYCW010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.091"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(135..620)
                     /locus_tag="ACHAYA_002220"
     CDS             complement(135..620)
                     /locus_tag="ACHAYA_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM8606004.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    710..1055
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1100..1741
                     /locus_tag="ACHAYA_002221"
     CDS             1100..1741
                     /locus_tag="ACHAYA_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8606005.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1760..2395
                     /locus_tag="ACHAYA_002222"
     CDS             1760..2395
                     /locus_tag="ACHAYA_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8606006.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2413..3228
                     /locus_tag="ACHAYA_002223"
     CDS             2413..3228
                     /locus_tag="ACHAYA_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8606007.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3547..4983
                     /locus_tag="ACHAYA_002224"
     CDS             3547..4983
                     /locus_tag="ACHAYA_002224"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8606008.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5172..7604
                     /locus_tag="ACHAYA_002225"
     CDS             5172..7604
                     /locus_tag="ACHAYA_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8606009.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            7834..8901
                     /gene="rlmN"
                     /locus_tag="ACHAYA_002226"
     CDS             7834..8901
                     /gene="rlmN"
                     /locus_tag="ACHAYA_002226"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8606010.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8891..9097
                     /locus_tag="ACHAYA_002227"
     CDS             8891..9097
                     /locus_tag="ACHAYA_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606011.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9094..10197)
                     /locus_tag="ACHAYA_002228"
     CDS             complement(9094..10197)
                     /locus_tag="ACHAYA_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8606012.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10749..11297
                     /gene="aac(6')"
                     /locus_tag="ACHAYA_002229"
     CDS             10749..11297
                     /gene="aac(6')"
                     /locus_tag="ACHAYA_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8606013.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3788 a         1936 c         2467 g         3232 t
ORIGIN      
        1 agaatactcg tattagtttt cagcgttcat actagttgtt acattgtttg attggccaaa
       61 agaatttctg atatattttg tgctaaataa agaaaaagac gcttgaattg ggaaataaga
      121 aaactactct atcattacaa attttcataa actggcagat gttctaagta aacaagtatc
      181 ttttcaaagt cggaaaaatc cataatcaac gtagtggtat tgatatttgg atgaaagcct
      241 acggtatctt gacgatcaat agcagcatcg atcacgactt ggattttttt ctctttgtcg
      301 tgcatcaatg ctaatggagt cactgtacct gctggaacgc ctaggaggtc aggaagctgt
      361 tcttcagaaa caaaagataa gcgcttttct tttaattgtt cagctagttg ttttaaatcg
      421 gcgtgctttt catcaggcaa gatgacaaga tagatttgtt ttccttttct tgatttcaat
      481 aatagatttt ttacttgcgg accaggtagg gaaaaaggga catttttgac tgacgtaatc
      541 gcaggatgat cgactttttg aaaagagata cccagttttt ttagtagttc ataagcttct
      601 tgttcggttg cacgactcat cgcgcatcac cctccttttt ttattagtgt atcataaaaa
      661 agagttgaca aatgaaaaat aatcatgtaa attacacgta aagttaaaac aaatgacatc
      721 gaaaagaaga gtagccgtta ggtgttttta gagagtctct ggttggtgga aagaggcagc
      781 agttaaccgt gaaacacatc ttggagtcga ttttctgaag cgtatagtag gaaaatccgg
      841 ggagcccgtt acagctgcgg tcgttgctag atgaccagat aaggctggct tttccgtgag
      901 gagtcggtaa aaaaaggtgg aaccacgtga ttaacgtcct ttgatcttcg gatcaaagga
      961 ctttttgttt tcatctgaac ggcaacgact gtgtgaggtt ttttctgcaa ggaaaaaata
     1021 aacagaggtg gaaccacgta taatacgtcc tcttggcatt tgccaagagg atttttttat
     1081 tttaaaggag gaaaagaaga tggagtattt gattgaagtt atgccgcaat tattagtagg
     1141 agcactcaca acattgaaat tatttttatt taccttgatt ggctcattac cattagggat
     1201 tgttttagca ttaggattgt ctttgcattt taagccgcta cgctacttgc ttcaaacata
     1261 tgtatgggtt atgcggggaa caccgctgtt gttgcaactg atttttattt tttatgggtt
     1321 gccgacgatt gggatcgttt ttgatcgttt cgaagcagca ctcattgcct ttatcttaaa
     1381 ctatgcagct tattttgcag agatctttcg tggagggata caatcgattc ctcaaggtca
     1441 atatgaagca gcgcaagttt tgcaattaag tccctttcaa acggtgacga aaatcgtcat
     1501 cccacaagtg acaaagattg ttttaccttc tgtagggaat gaagttatca acttggtcaa
     1561 agattcttcc ttgatctata tattaggttt aggtgacgta atgcgtgctg ggaaaatcgc
     1621 gatggaacgc gacgcgactt tactgccttt gctaggcgtg gcaatcatct atcttacatt
     1681 gactggtatt ctaacaatcg taatgaaaca agttgaagga aaattaaatt attaccgcta
     1741 actgatagga ggaagaaata tgattgaatt gaagaaagtg aacaaagcat ttggtgaaaa
     1801 aaaaataatc aaagatttga atctaatcat tccagatggg aaaacattag ctatcgtagg
     1861 tccttccggt ggcggtaaaa ccacgctttt acgtatcttg gctggcttag aaagttctga
     1921 cagcggagaa ttttatttag acggtgtacc gttcaaccca ttcgagctta aaatgaaaga
     1981 acaagttgta ggagtcgtat tccaagactt ccagctattt ccacatctga atatttttga
     2041 aaacattacc ttagcaccaa aaatggtatt gaaacagaca aaagaagaat acaccaaaaa
     2101 agcagaagaa ttagcggctt cattagggat tagtgattta ttgaatatct atccttatca
     2161 gctttctggc ggacaaaaac agcgggtagc gattgccaga gcattggcta tgaatccacg
     2221 ggtattggct tatgatgaac caacaagtgc tttagatcct gaattacgcc agcaagtcga
     2281 gcgtttgatc ctatcattga aagaagatgg ggtaacccaa attattgtca cccatgattt
     2341 agtatttgca gaaaatgtcg gtgatcaatt actaaaagtt tccccaattc attaagaaag
     2401 gagaagatag agatgaaaaa aaccagtttt ctattcgctt tgatcgcagg tcttcttctt
     2461 tttgcaggct gttcgaacaa aaaaacttca gcagataatt gggcgactat caacgaagag
     2521 aaaaaaatcg ttataggttt agacgataca tttgttccta tgggatttag agataaagac
     2581 gggaagctca caggatttga tattgattta gccaaagcag tctttgaaca atatgggatt
     2641 acggtagact ttcagcccat tgactggtca atgaaggaat ttgaactgaa caatggaacg
     2701 atcgatttga tatggaacgg ctattccaaa acgccggcaa gagaaaagaa agtgcaattt
     2761 acaaaacctt atatggaaaa cgatcaagtg ctgatcacac ctaaagaatc aggaatcaca
     2821 aactttggac aaatgaataa taagagatta ggtgcgcaaa atgggtcatc tggttatgat
     2881 gtatttacga aacaaccaga agtattaaaa gagatagtca aaaaccaaga tgctgttttg
     2941 tatgatagtt ttaatgaagc attgatcgat ctgaaaagca gacgggtcga tggtctgctg
     3001 atggataaag tatacgcaga ctattatttg aaacaaagaa acgaactaga agacttcaac
     3061 ataacaaaag gttcttatga gagtgaggat tttgcagttg gtgcacgcaa gtcagatagt
     3121 acattagtga aaaaaatcaa tgaagcccta gatcaactag agaaaacagg tgaattccaa
     3181 aaaatatccg ataaatggtt tggcgaagat gttacgccga gaaattaaca aataggggac
     3241 tgtgacaaaa gacgtgtcac agtccctttt tccgaataaa cagtgttctc ctgccaactc
     3301 cttgtgactc ggagctgaat ggcaggctca caacctcttg gtaaacggtg ttcaaaagcc
     3361 agtttttcgg tattaagcca aatttatgca aaaatgtaga aagccatttt cacaaaattt
     3421 ttcttactac tcgaaactaa gcgacttttt cacaacctcc tttacataat tttattttgt
     3481 tgtggaaggg gtttcgtgtt attctaaaag aggaacgatt gaaggttata ggaaaggaag
     3541 agatgaatgg aacacaaaga acttacctca tttccaaaag attttctgtg gggctctgct
     3601 tctgcagcgt atcaagtaga aggagcttgg caagaagatg ggaaaggaga atcagtctgg
     3661 gatcgatttg tacggatacc tggaaagaca tttaaaggaa cgaacggtga tttggcagta
     3721 gaccactatc atcgttataa agaagatatt gctttaatga aagaacaagg tctgaaagct
     3781 taccgcttct ctgtagcttg gacacggatt tttcctaatg gacgaggaga agtcaatcaa
     3841 gctgggctcg ctttttatga gagattgatc gacgaattga ttgaaaacga tattgaacct
     3901 atgttgactt tatatcattg ggatcttcca caagcattgc aagatgaata taatggttgg
     3961 gaatcacgtc agatcattga agatttcact cattatgcag aaacattatt tgaggcattc
     4021 cgcgggaaag tccattactg gattagtttg aatgaacaaa atatcttcac ttcattaggt
     4081 tatttgttag cagcacatcc gccaggtgtg acagacccga aacgaatgta tgaagttaac
     4141 catattgcta atttagcaaa tgcatctgtc atcaataaat ttcacgagtt agaaatgcct
     4201 ggaaaaatcg gaccaagctt tgcgtatacg ccaaattatc caattgacag taatcctgaa
     4261 aatgttctgg ctgctgaaaa tgcagaagac ctgatggcca attattggat ggatgtctat
     4321 atgtggggaa aatatccgat tgctgccatg aggttcttag aagaaaaagg atgggcacca
     4381 acgattgaag ctggcgatgc agaattgcta gaatctgcta aaccagattt tcttgggatc
     4441 aattactatc aaacggcgac gaatgcattc aatccattaa acggagttgg tgctggaaaa
     4501 atgaatacga caggaaaaaa aagttcatca gaagaaactg gagttcctgg aatgtataaa
     4561 aaagttgaga atccatttgt cgaacgaaca aattgggact gggagattga cccagaagga
     4621 ttaaggatcg gactgcgtcg tatcacaagc cgttaccgca tgccggtact gattacggaa
     4681 aatggcttag gagagtatga caagttgaca gaagataaac aaatccatga cgactatcga
     4741 atcaattact tacaaagcca cgtcaaagcc atcaaagaag caatttcgga tggagcagaa
     4801 gtcctaggct attgcacctg gtcttataca gatttgctta gctggctgaa cggttatcaa
     4861 aagcgatatg gatttgtata tgttgatcaa gacgaaacac aagaaggatc tttggaacga
     4921 tataagaaag acagttatta ctggtaccaa aaactgattg aagaaaatgg agagaacctt
     4981 tagttcacat acttaaaaca agaaggtacg gctggatatg gccgtgcctt ttcttgataa
     5041 aatcatttag aaattaagaa aaactgtaaa ataagctgaa aaatgaaaga aagcgtcagt
     5101 tctttcaaac aaaactttag ttcccttact tttttttgta taatgaaact tgatgaaatg
     5161 agggataata aatggcacaa aagaaacggc ctactaaaaa aaggaaaaag acgaaaaaaa
     5221 cgaaaaagca aaaacagcag caggaacaaa tgctgacgat tggtgttggg tttgccttga
     5281 ttttatttgc tgtcttcggc tttttaaaat taggtttttt aggaatattg attgcaaacg
     5341 gctttcggat cattgcagga aatacttacc aaatcctctg tcttttatta gccgtattag
     5401 gattttggat tgtcattaag aatacagagt tttcaattgg taaaaaccga cgctggtttg
     5461 gcggcatact cttttatttc ggtatcttat tattgttaca tgcccattta tttggcaaac
     5521 tccatactgg ggaaccaaat atcatgggga cgacatggga tttgttagcc agtgatataa
     5581 aacaaagtca agtagacaat aacgttggtg gtgggatgat tggagcaatc ctttatcatt
     5641 ttacttactt cttgattgca cagcctggaa gttatttagc agcagtgcta ttactggcag
     5701 gaggagcatt cctgttcagt aatctggaag gctatcagct gttaaatggt atccaatcaa
     5761 ttggagaacg agtacaggaa ttattggaag gtgatcctgc caagcaagct cgaaagcaag
     5821 cggcaaagga agagagaatg aaacagcgcg cagaagcgaa agaagcaagg cgcttagctg
     5881 caaaagaagc tgcagaatat gagaagaaaa aagcggtgag ccagcaaagg gaaaatactg
     5941 ctgatgaatg gcaggaacca gaaaatacag agccggagca gctaagtttt gttccaatcg
     6001 acagttttca agaaaatatc catcctgcga atctagagaa accagtacca gatacaccaa
     6061 aacagaccaa tacagcagaa ggtttttcag atgagttgcc agaggatgac ggtacctcat
     6121 tagaatttga gattgaagca gaacaggaaa atcaagacta tgaattaccg acagttgatt
     6181 tgcttgattc tataccaact gtggatcaaa gtgatgagta taaaaaaatt gaaaaaaata
     6241 tcggcgtatt agaacaaact ttccaaagtt tcggtgtcga tgccaaggtc gtcaaagcca
     6301 gcttaggacc atccgtcaca aaattcgaag tccagccggc tgtaggggta aaagtaagta
     6361 aaattgtgaa cttaacagat gatatcgcgc ttgctttagc ggcgaaagat gtccggatgg
     6421 aagcaccgat tccaggtaaa tcactgattg gtattgaagt accaaatgga aagatcagta
     6481 tggtttcctt tagagaaatc atcgaagcac aaccaaacca cccagataaa ttactagaag
     6541 tacctttagg acgtgatgtt tctggacggg tacaaacggc tgatctttcg aagatgcctc
     6601 acttactcgt ggctggttcg actggtagtg ggaagtcagt agcaattaat gggattatca
     6661 caagtatatt aatgcgtgct aagccccatg aagtaaaact gatgatgatc gatccgaaaa
     6721 tggttgaatt gaatatgtac aacggcatac ctcatctgtt gacgccggtc gtgacgaacc
     6781 ctagaaaagc agcgcaagcc ttacaaaaag tagtacaaga aatggaagaa cgatatgaaa
     6841 aattcgcggc aaccggagta agaaatattt ctggttacaa tgaatttgtt caacagaaaa
     6901 acttggaaaa cggaacgaaa catccaacct taccattcat cgtagtgatc gtagatgaat
     6961 tagctgattt gatgatggta gccagcaatg aagttgaaga tgcgattatt cgtttagcgc
     7021 aaatggctcg ggcagcgggg attcatatga tcctagctac tcaacgtcca agtgtggatg
     7081 ttatcacagg aatcatcaaa gcaaatgtac cttccagaat ggcatttgcg gtatcaagcg
     7141 gtacagattc tcggacgatc attgattcta atggggcaga aaagctctta gggcgaggag
     7201 atatgctatt cttgccaatg ggtgagaata aaccgatccg tgtgcaagga gcattcattt
     7261 ctgaccacga agtagaacgg gtcgttcaat ttgttacaga tcagcaagaa gctcattatg
     7321 aagagaaaat gatgccgaca gatgaagttg aaacgacagg tgctccagaa cagccacaag
     7381 atgagctgtt tgaagaagca aaagcacttg tcgttgaaat gcaaacggca agtatttccc
     7441 ttctccaaag aagatttcgg atcggttaca accgagcagc acgtttagta gatgaactgg
     7501 aagctcatgg agttgttggc ccgtcagaag gaagcaagcc tcggaaagta ttcattgagc
     7561 aggaagaaac atcagaaacg ttgccggacg ataatgacat gtaaaacttg tgtgatgaag
     7621 ggttgaaaaa cgaataacca ataaaaagac aaggcatgat gataccaaaa gaaaaaatgg
     7681 tttttttcca tcttttctaa tacggtatga tgtcttgtct ttttagtttt tgtgagatta
     7741 aagttctatt cgatttattt gtgaagtatt gcattaagga aatgaatcat gtatagtatg
     7801 tagcgaaact ttgagaaaaa gagggaaaat actttggaaa aacagtccat ttatggcttg
     7861 acgaatgaag aattaatcaa ttggtttata gaaaacggcg agaaaaaatt ccgagctgcc
     7921 caagtttggg aatggttgta tcaaaaacgt gtaagtaatt tcacagaaat gacgaatcta
     7981 tcgaaacaat tgatcgaaaa attatcggag cattttatca tcaatcctct aaaacagatg
     8041 gtcgtccaag aagcttcgga tggtacagta aaatatttgt ttgaactccc tgataaaaac
     8101 atgattgaga ctgtactgat gagacaagaa tatggcttgt cagtttgtgt gacgacacaa
     8161 gtaggatgta atatcggctg tacgttttgt gcaagcgggc tgctcaaaaa gaaccgtgat
     8221 ctgactgctg gagaaattgt tgcacagatc atgatggtcc aacattattt tgatgagcgc
     8281 aatcttggcg aacgcgtttc acatgtcgtt gtaatgggga tcggtgagcc atttgataat
     8341 tacgacaatg taatggactt tttgcatatc atcaatgatg ccaaaggatt ggcaattggt
     8401 gctcggcaca ttacagtttc cactagtgga ttagcacaca aaatcaagga attcgctaat
     8461 aacggcttgc aagtcaatct ggctatttcc ttgcacgcgc cgaataatga ggtgcgaaca
     8521 tcgatcatgc ggatcaaccg aagtttccca attgagaaat tgatggaagc tgtggatgag
     8581 tatttagaga aaacaaaccg tcgaatcacg tttgaatata tcatgttgaa tcaagtaaat
     8641 gatagaccag agcatgcgca acaattagca gatctattaa aagataagaa aaaattagca
     8701 tatgtcaact tgattccata taatccagtc agtgaacatg atcaatattc tcgcagtcca
     8761 aaagctgatg tattgagatt ttatgatgta ttgaagaaaa acggcgtaaa ctgtgtgata
     8821 cgtaaagaac atggtacaga tattgatgca gcttgcggac aattgcgaag caagcaaatg
     8881 aaaaaaacag ttgccaaata atcgtatcct actcgttttt tcaattacca taaaaggagc
     8941 agttttctta attaaggaac tgttcaactt aaataagaga aaagattcta tagcgaaagc
     9001 tataaagctt tttttgacaa gaaaaaccct catcaagcaa agaaatgtcc tcaatttgat
     9061 gacagcctgc ttgatggggg ttttagcaat tatttaactg attttcttat atccttgctg
     9121 gctttcttgt gtagcatgat aagtttctac gtcaagaatg gcctgtttaa atttttctcg
     9181 ggtgatttca taaggcacat gtttaatatc atttgttgcc atggctttat ctaagattgc
     9241 ttctaaatcg gtcgtgacat tgagattcat gtcggttaac gatactggaa gttggttctt
     9301 tttcataaac acatagattt tatcaaacaa atcgaactgc tgatccatca tcaataaggt
     9361 cagtacacca taactgacag atgctccgtg aaggtatccc tccatttttt ctagaacagt
     9421 cgtaccgtaa tagaatgagt gtgccatagc agaattatag ttattgttaa ctaaaacaga
     9481 aaccaatccg gtagtaacaa tgatatccga aacgacttgt tcaattgcat atgagggacg
     9541 atcgtcggca ctatctttca atgcttgcgt accataagtc aagagacgat caatgcacat
     9601 ttttccgatt tggattccta atctatccgt gtagcctaga tcatcgccac gagcagaaaa
     9661 agtagattcg acttctttgg ataaagcatc gccaatacct gcccataagt atttatcagg
     9721 tgcttcggca ataattgctg tatcaatgaa ggtgtgtaca ggtggttttg aaggaaagaa
     9781 gagtccgtcc attacgcctt cttcgttata gatgacacat accgcagtga cgggcgaaca
     9841 gttcgaacca atagtgggaa aggtgaagac tggtttagct agtttttcac caatgacttt
     9901 tacagtatcg caagcacggc caccaccgac tgcaaacagg acgtctgctt tttggacact
     9961 ttcatgctgg atcaactgtt caatatttcc ataagttgaa ttacccccgt accagagact
    10021 ggcgacaacc tctatttcct gtgtcaaagc tacttttatt ttgttttctg cctttgaaag
    10081 tgcagtcttc cctccaatca gcactacttt tgtcccatat tctttgacga ttccatttgc
    10141 tttttcatac gcatctgttc caatggtata atttggtaag aaaacacttt gactcatatt
    10201 catttccttc tttcttcgtt gaaacgggtt aaaattttaa agagtgatcg atctgactgc
    10261 ggctgtagat ggagtagagg tgaacctaag acaggataag tgatgaataa ggtggcatga
    10321 acaccttgaa atcatcaata agtatcctaa aaacgaacaa gtacaaaaac attgaaaacg
    10381 acaataatga atttttgttc ctcttagtat ttctaccgat tccctttgct tttttgtaat
    10441 tatctaaaaa ttctaaaaat taagattatt atatgaaaac aggttttatt tgtcaattca
    10501 taattggttt tttttaaagc aaaagataat ctatatcatg atgaacaaac agagtttttt
    10561 cctccaattg atggctttcc tgatctttcc tattttgaat aaagtgtttc tgtataattt
    10621 tttattttat aagtaagata aataatgaaa gaatagagat actgattggt agctaaagat
    10681 ttttgagcga gaacatttcg ttttttcaaa aggactgtta gaataagcaa gaacaagggg
    10741 gaagacgtat gataatcagt gaatttgacc gtaataatcc agtattgaaa gatcagcttt
    10801 ctgatttact gagactgact tggccggaag aatatggaga cagctcggca gaagaagtag
    10861 aagaaatgat gaatccagaa cgaatcgcgg tagcagcggt agaccaagat gagttagtag
    10921 gatttattgg tgcaatccct caatacggta tcacaggttg ggaattgcat ccattagttg
    10981 tagaaagctc ccgacgaaag aaccaaatag gtactcgatt agtcaattac ttagaaaaag
    11041 aagtagcttc cagaggagga atcacgattt atttaggtac ggatgattta gaccatggaa
    11101 caacgttaag tcaaacggac ctgtatgagc atacatttga taaagtggct tccatccaga
    11161 accttcgtga acatccgtat gaattctatg aaaaattagg ttataaaatc gtaggtgtct
    11221 taccaaatgc aaatggctgg gacaaaccgg atatttggat ggcaaaaacg attattcctc
    11281 gaccaaattc tcaataataa aaagaggttg tgacagaagt aatcagcttc aagaaataag
    11341 aaggaattta cgaaaattac ttcttgaatt tttgtgaaat tccaacttat ttccgaagaa
    11401 gctacttttg gaacaccgtg tac
//
LOCUS       DAYYCW010000090        11226 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000090 DAYYCW010000000
VERSION     DAYYCW010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.092"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            188..1447
                     /gene="lysA"
                     /locus_tag="ACHAYA_002230"
     CDS             188..1447
                     /gene="lysA"
                     /locus_tag="ACHAYA_002230"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM8606014.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1434..2234
                     /gene="dapF"
                     /locus_tag="ACHAYA_002231"
     CDS             1434..2234
                     /gene="dapF"
                     /locus_tag="ACHAYA_002231"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM8606015.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2237..3118
                     /gene="dapA"
                     /locus_tag="ACHAYA_002232"
     CDS             2237..3118
                     /gene="dapA"
                     /locus_tag="ACHAYA_002232"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM8606016.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3115..3831
                     /gene="dapB"
                     /locus_tag="ACHAYA_002233"
     CDS             3115..3831
                     /gene="dapB"
                     /locus_tag="ACHAYA_002233"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM8606017.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3837..4532
                     /gene="dapD"
                     /locus_tag="ACHAYA_002234"
     CDS             3837..4532
                     /gene="dapD"
                     /locus_tag="ACHAYA_002234"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM8606018.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4548..5549
                     /locus_tag="ACHAYA_002235"
     CDS             4548..5549
                     /locus_tag="ACHAYA_002235"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM8606019.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5546..6907
                     /locus_tag="ACHAYA_002236"
     CDS             5546..6907
                     /locus_tag="ACHAYA_002236"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM8606020.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6867..8018
                     /locus_tag="ACHAYA_002237"
     CDS             6867..8018
                     /locus_tag="ACHAYA_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8606021.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7993..9162
                     /locus_tag="ACHAYA_002238"
     CDS             7993..9162
                     /locus_tag="ACHAYA_002238"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8606022.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9337..10716
                     /locus_tag="ACHAYA_002239"
     CDS             9337..10716
                     /locus_tag="ACHAYA_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8606023.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10997..11226
                     /locus_tag="ACHAYA_002240"
                     /pseudo
     CDS             10997..11226
                     /locus_tag="ACHAYA_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3738 a         1955 c         2503 g         3030 t
ORIGIN      
        1 taaatagctt ctaatagttc gggtattttg tttttataac catttttatt cttattttta
       61 aaaaaatatt tcgaaatata ctagacaatt cagaaaattt tgtttaatat tattccaata
      121 aaattcaaat ctttatcaaa tgaaattcca ataaaaacgg aaaaaaagtg aaaagaggaa
      181 tgaaaaaatg acagaattga taattggcgg agtcacagca agcacattgg cagaaaagta
      241 taagacacct ctgtatgtgt atgatgaaga aaagatgaga caaacgatgc tgtcattcaa
      301 gcgaggcttt gtatcgaaac gttttgatac aaaagtgctt tacgcctcta aagcattcca
      361 aacgattgag atgctgcatt tagttcgtga atatggattc gggctagatg ttgtcagtgg
      421 tggggaaatc tataccgctt tgcaagctga ttttcctaga gatactatct acttccatgg
      481 taacaacaag acgccagagg aactgatcta tgcacttgaa aatgatttgt tgcatattgt
      541 tgccgataat ttgatggaag ttgaattatt agcagaatta agccaacgct atttaaaaaa
      601 aatctgtatc atgcttcggt tgaatgtcgg agtagaagca catactcatg aatatatcgt
      661 cactgcgcat attgattcaa aattcggcat gagctatgaa agtaaagagt gccaacaagc
      721 ccttcagttg ataaaagaaa gcagctatct agagttagaa ggatttcatg ctcatatcgg
      781 gtcacaaatt tttgatgtta ctgcttggtt agctgaaatc gacaaattga cgaattactt
      841 aaccgatttt gaccaacctc tttccttgaa tcttggcgga gggtttggga ttcgttatac
      901 acaaaatgat cagccgatcc cagtcgaaga ggcactaaaa aaactgattg aatatacgga
      961 agaagctttg gaaaaacgaa aactgaccat tcgtcaatta ctgatcgaac cgggtagaag
     1021 tattgttgga gaagctggta caacactata cacgattggt ttcatcaaaa agacaccaca
     1081 taaaaaatat tactttgtag atggcgggat gacagataat atccgtccag ctctgtatca
     1141 ggcagaatac gattgtgatt tagcagcaaa actgtcggaa gaaaaaacag agaaagtgac
     1201 gattgcagga aaaatgtgtg aatcaggcga tgtggtaatt aaagaagctt atttgccgaa
     1261 agcagaatcg ggtgatcttt tagctgttta ttccacggga gcttatggat actcgatgag
     1321 cagcaactac aatcgagcga cacgaccagc agttgttttt gtcaataatg gccaatcaag
     1381 actgattgtt cgcagacaag cattttctga tctactaagg ggggaagttt cctatgatcc
     1441 ttcataaata taatggctgc ggaaatgatt ttatcttatt agattacgca gaagacacag
     1501 attatagtca attagccgct tctttatgca caaaagaaaa atatgatata gacggattga
     1561 tcgccgtaaa aatcgatccg ttggaaatga tctattacaa caaagatggc agccgcgcgc
     1621 cgatgtgcgg aaatggcatt cgctgttttg ctcgatacgt aaatgatcag aacatgatga
     1681 atgaacagga attcaacgta gagacattag caggcacaag aaaaattacg gtgttggatc
     1741 cctcaccttt ttattgcagc gtagatatgg gagtgccaga tgattctccg gaaaaaatca
     1801 aagctaatca aacttatcca ataaaaaacc aggaaataga gatcaatggt gaaaaagtaa
     1861 cgatcactag tttattttta ggaacgattc ataccgttgt ttttgttgaa aatgcgcgtg
     1921 aggaagtctg gagaaaaaga ggtgaactga tctgtcatca tcctttgttc aaagaaaaaa
     1981 ccaatgtcaa ctttgtagaa gtagtcaatg aacaagaatt gatcgtccgt acctatgaaa
     2041 gaggagtagg ttggacactt gcttgcggta caggttgctg tgcctcttat gtggttgcta
     2101 aggaaactgg gaaaatttca gcagatcagg tagttgttca cttggaacaa ggagacttga
     2161 tgatctctgg tgaggatgcg atacaaatgg ctggcccagc agttcatgaa tggacaagaa
     2221 tattggagga atgaagatga taaaaggttc gatcgtggcg ctgatcacgc cgatgaatga
     2281 agagggatca gtagattatg ctggtttgga aaaattgatc caattccatt tagatgaaca
     2341 aacagatgga ttattagttt taggtacaac aggagaatct tctacgctca cacaatctga
     2401 ggaggagcag atccttcaac tgacagtaaa gaaagtggct ggccgtgtgc cggtgattgc
     2461 aggagctgga acgaataata cgaaagaaac gattgaaaaa gcgaagcatt ttgcttcttt
     2521 gggggcagac gcacttttgg tcatcacccc ttactacaac aaaacaagtg atgcaggact
     2581 agctgctcat tttacagcaa ttgcagaagc ctcaccgatt ccgcttattt tgtataatgt
     2641 tccttctaga acaggcatgt cgattccaat ccatgtatta gtaaatttag cagaacatcc
     2701 taatatcatt ggtttgaaag aagcctcagg agatatggct tatgtgatgg atgccgctcg
     2761 gttgattgga gaagaattct tcctttacag tggaaatgat gatttgatcc taccagtaat
     2821 gagtgtagga ggaagcggtg taataagtgt atgggcgaat atccaaccta aaatagtcca
     2881 tgaattagta aaagatacgc aggacggaag atggcagcaa gcaaaagaaa agcaattaaa
     2941 tgcacttgaa ctgatccatg ccttgttcag tgagaccaat cctattcctg tcaaagcagc
     3001 catgtcatta ttagacttac cttccggacc tctacgcctg cctttagttt ctctttcaaa
     3061 agagaaaaag aaacaattag ctcagctttt gttaaaagaa aggacggttg aaaaatgaaa
     3121 atcggactga ttggcagtgg acagatggga aatagagtcg aagaagttgc acaagaaaga
     3181 ggagatacgg tccttctttt acggacggca cctaaagaag caactacgct tgcttttacg
     3241 gaactgccag aactgcttat tgatttttct catccagata atttagagaa aatcctttcg
     3301 tatagcttat catatcaact tccgttagtc attggcacga caggttatac acaatcacag
     3361 ttccaagaaa taaaagaaca tgccaaacat gtacctgtga tgtatagtgc taatttttcg
     3421 ttcggcatca tggtgatgaa tcaactaatc aagcaagcag ctgcaatgtt gcaaggctgg
     3481 cagatcgaac tgattgaaaa acatcatagt cgtaaaaaag atgcaccatc tggtactgca
     3541 aatatgctgc ttcaaacaat ccaagaagtg caaaagctgc agcccgtata caactggcaa
     3601 gggaaaaaaa gagaagaaaa ccaaattggt gtacacagta ttcgtgcagg ttctttacct
     3661 ggtgaacatg atgtattgtt tgctgctact gatgaaatat ttacgatcaa acatgaatct
     3721 ttttctaatc gaatctttgc tgcaggcgca gtagaagcag cggactggtt gaaagatcag
     3781 tcaccaggtt attacaaatt agaagattta ttaatggaca aaggggtgta aaaaacgtgg
     3841 aagctcaaga aatcatccgc tacattcaaa cggcatctaa aaagacgcct gtgaaagtct
     3901 atctgaatgt aaccgagcca atcgaattta aagaaagtaa agtttttggt gaaggaagca
     3961 gcttcgttgt ttttggtgac tatgagacga ttgctccggt tttagaagct gaatcagaaa
     4021 aaatcattga aatcgaaatc gaaactgctg caagatacag cgcagttcct ttgctagata
     4081 ttaaaaaaat taatgctcgt atcgaaccag gtgctattat cagagatcaa gtgtcaatcg
     4141 gcaacaatgc cgtgatcatg atgggagcga ttatcaatat tggtgcagta atcggtgaaa
     4201 acacgatgat tgatatgggt gctgtcttag gtggacgagc gacagtgggc aaaaactgcc
     4261 atattggtgc aggagctgtc ttagcaggtg tgatcgaacc agcatcggcc aaaccggtta
     4321 tcgtagaaga cggcgtactg gtaggtgcga atgcagtgat tgttgaaggt gtccatatcg
     4381 gaaaagatgc agtcgtggca gcaggagcag tggttttaga agatgtggca gcagaaactg
     4441 ttgtcggtgg aatacctgca cgtgtattaa aaataagtga tgatcagaca aaagaaaata
     4501 cagcattgat tgctgcgtta cgcgaattat agaggagatg gagaaaaatg cgcgaaatag
     4561 atacctgtat tgtgggggca accggattag tcggccaaac gatgctcaaa gtgttagaag
     4621 aatatgattt tccagtagga cgattgaaat tattagcttc aaaacgttca gccggtaaag
     4681 tttgtgtatt caagaaaaag aaacacactg tacaggaatt gacagaaact tcatttgaag
     4741 gatatgattt agcgctattt tctgcaggtg cttctgtatc caaagagttt gcgccaatcg
     4801 cacgaagtca gggactattt gtcatagata attcctcagc atggcgggaa gattcggata
     4861 ttccattaat cgttccagag gtcaatctgg aagactatac attgaatcgg ctgattgcaa
     4921 atccaaattg ttcaaccatt caagcggtac ttccgcttaa agctttacaa gaagcctttg
     4981 gtatccagcg agtcaattat tcgacctatc aagcagtttc tggttccggg caaaaaggca
     5041 ttgaagatct tcatcgtacg caaaatgacg aggaaccagt tttgtatcct tataatatta
     5101 gccaaacggt gattccggaa atcgatcagc ctttggaaaa tgggtataca aaagaagaac
     5161 agaaaatgat aaatgagacc cgtaagattc tgcatcagcc tgctttgcca gtatcagcaa
     5221 cctgcgtacg tgtgccaatc gaaaatggac atggagtctc tgtggcagtt caactgcaaa
     5281 aagagtttac gacggaagaa attagagagt gtctcagcca gtttccaggg attcaattag
     5341 aagatgatct agcagcacat aaatatccga cgagcattct tgctagagga acagatatgg
     5401 tttatgttgg gaggatcaga aaagacactt ctttagagaa cggattgttg ttctatacga
     5461 ctgccgataa catacgcaaa ggagccgctg cgaatgccgt acagattgct gcagctttaa
     5521 tgcagaaaat agcggaggtg gcaagatgag taatacgctg aaagtagcga aattcggcgg
     5581 aacatcgatg tctcatgaag agcaattcaa aaaagtccgt acaattgttc tttcagatcc
     5641 caatcgccaa gtgatcgttg tttctgcttt aggcaaacgt acgtcagatg atgacaaagt
     5701 aacagactta ctttatttga tttatgctta ccttcagcat catgttgatt ggacacccct
     5761 ttggaaaagg atttgtgatc gtttcattca agtaagagat gttcttgccc tgcagttacc
     5821 tattgaagat gaactagcac gcatacagaa acaactggaa gacgaaaaga taactgaaga
     5881 ttatttagta agtagaggtg aatatctgac tgctcaactg atggctgagt atcttggtta
     5941 ccagtttatt gacgcaaaag acctgatttt ctttgactat gatgggaaaa tcgatcagga
     6001 aaaaacagca caggaaatca caaaagcatt taaaagatat ccgaaaatgg ttgttccagg
     6061 gttttacggg gcgaaccctg caggaaaaga aaaattattg ggccgtgggg gaagcgacat
     6121 taccggcgcg atactggcga agatgttgca agcggaaagt tatgagaatt ggacggatgt
     6181 ttcaggaatc atgatggctg atccgcgaat catcgatcat cctaaaatca tcgaggaaat
     6241 ctcttttaga gaactgagag agatggctta tatgggggct aacgttcttc atgaagcagc
     6301 tgttttccca gttcaagagg ctgatatccc catccaaata aaaaatacca atgatccttt
     6361 cgctcaaggg acaaaaatat cgaatcacga aatagaaaag gaaaatggac tgacaggaat
     6421 agctggacgc aaggattttc tttccatcac tttattcaaa cgtcatatgt ccgatgaaat
     6481 cgggtttatc tggaaagcaa tgagcatttt tgctaaacat gagatatcaa tcgaacatat
     6541 tccttcaggg atcgacaata tcggagtgat cgtctcagca gaagcgattg ccgataagtt
     6601 atttctcatc acgaaagaat taaaagaaac acttggcgtg gaagaaatcg tcgtgatcga
     6661 agacttagct ttgatctctg tcgttggggg tccgcataaa gaattgattg gattatctgg
     6721 gaaagtgtta tcgatattga acaaattaga aattcgcaca tcgatcttat cacaaggagc
     6781 acaggagttg aatctgatca tcggtgtacc gaataatcaa tatgaaacag tagtgaaggg
     6841 tatctatgaa gggatggtga atacggatgc tgaaagaata tcgaatggca ctccatcaaa
     6901 ttcctgaaat cgggtttcat gaattcaaga caaaaaaata tttatatgaa caagtaaaaa
     6961 acagcgaggc cgttatccat gaaatcggcg aaacaggact actcctttat tttgataatc
     7021 agcaagaaga aacgattgct tttcgaacag atatcgatgc actgccagtc aaagaagaaa
     7081 cgaatctctc ttttgcttct aagcatgaag gttatatgca tgcatgtggt catgacggac
     7141 atatggcgat gcttttaggg ttgactgact ttataactaa acacattgag gatcttattt
     7201 ataatgtagt actgatctat cagccatcag aagaaatttc cggtggcgca caaagcgtta
     7261 ttcagtcagg attgttggat aaatatgaag tacaagcaat atttggcttt catctatggc
     7321 ctggactagc taagggagaa gttttctctc gaccaggacc tttgatggca caaagcagtg
     7381 aaacagacat tatcattaaa ggccagtctg cacatattgc ttctagtgaa aaaggaatcg
     7441 atagtctgga ggcagctgta cgatttttga agaatgttta tgattttgac cggcaattac
     7501 cggcagagtg gaatcatcta atgaaatttg gccaaatcca aggtggaacg atccgaaatg
     7561 ttttagctgg agaagttatt gtatccggaa gtatccgttc ttacagttca gatatgcaaa
     7621 attatttgaa gaggggatta gaccatttag cagaacagtt tcactctaca gctactattt
     7681 cttttcgcta taatgacggc tatccagctg taatcaacca tagaggctta tatgcagcac
     7741 tgttagattc aaacaaacta catgaattag atgctccagt tcttcaagcc gaagattttg
     7801 gcatatatac gcaacaatat ccatgtgtat ttttcttttt aggtgtcggt gatacccctg
     7861 ctttacatga tccgacgttc gattttgata tggatgtttt agaaaatggt gtagaatggt
     7921 acaaaacgat tctttttaca gaaaatcttc ttgtaaaaat cgatagcagt tcaaaagagg
     7981 agcgagtaaa taatgatgaa tttatccaat cgatttagtc ctaggctttc tagaatagaa
     8041 gtatcaaaaa tacgacaatt cgatcagcaa atatcgtcga taccagatgt gatcaaactc
     8101 accctgggag aaccggattt tccaactcct gaacatgtca agcaagcagg aatagcagcg
     8161 attgaagaag acttttctca ctataccgga atgagaggat tagaggaact gcgggaagcg
     8221 gcttgtatat ttcagcaaca gcgttatgga ttaacttatg atccgcaaac ggaagttttg
     8281 acgacagttg gtgcaacgga agcgattgcc tctgcgttgt tatctgtttt ggaagaagga
     8341 gacaaggttc ttattccagc gcctgcttat tcaggatatc agccattagt ggaactagct
     8401 ggtgctgaat tgatcccgat agatacttct gacacagggt ttgtctgtca gccggaacaa
     8461 tttgaacgag catttgaaca atatggatca gctgtcaaag cagtcatttt aaactatcct
     8521 aacaatccta ctgggacgac attgtctgca aaacaattga aagcaatcgc tgaggtttta
     8581 aaaaaatatc cggtatttgt tataagtgat gaagtatatg ctgaattgac ttatagtgga
     8641 acgcatatgt ccatagctac ttatcttcca gaacaaacaa tcgtgataag cgggttatcc
     8701 aagtcacatg cgatgactgg ttggcgagtg ggctttattt ttgctcaaaa agcccttatt
     8761 gacgaactaa ttaaagtcca tcaatatctg gtgactgccg ctacaacgat gtcgcaaaaa
     8821 gctgcggctg aagcgctaat aaatggggtg aaagatagcg agaatatgaa agagcaatat
     8881 caaacacgaa gagactatct ggtccagcaa ttggcgcctt taggatttga agtgacccag
     8941 ccaaacggtg cattttactt gttttgcaaa ctcccagctg ccattcaaac tgactcttgg
     9001 cagttttgtg tagaattagc ggaacaggca aaagttgcgt gtatcccagg aattgcattt
     9061 ggcccagaag gagaaggtta tatccggatt tcttatgcta gcagcatgga aaaattacat
     9121 gaggcctgca aacggatcaa agacttttta acagaacagt gattcataga ttttatcaaa
     9181 aaaacaatcg ttaattttgc ggtatattct gcaaggagac gattgttttt taaatttagt
     9241 tcaaatgaac gcgtttcttt tgattcataa ctaatgtaat gttaagttta aattgtttag
     9301 aatgatttta aaagaatatt caaggaggtt tcatcaatgg aaaaaatcaa agttgtcatt
     9361 gtaggtgcat ctcatggagg gcatcaatcg atcttggaac tattgacacg atacggtgaa
     9421 aatgtagata tcacgctatt tgaagcagga gactatattt ccttcatgtc atgtggtatg
     9481 gagctgtatt tagaagacaa ggtaacagat gtaaatgatg tacgtaattt cagaccagaa
     9541 aactttccgc aatccaatgt tcatatttta aataatcacg aagtaaaaac gatcgatgcc
     9601 gataaaaaaa cagtaacggt tactagcgca tcagataacg aaacaaaaga atatccttat
     9661 gacaaattga ttttgagttc tggtgtcaaa ccaaacaatc tgccagtacc aggagcagat
     9721 ctagaaaatg tctacttgat gcgtggatac aattgggcaa ccaaaatcaa agaaaaatta
     9781 aatgatccgg ctattaaaaa aatcgctgtc gtaggtgcgg gttacattgg gatcgaagca
     9841 gcagaagcaa gccgaaaagc tgggaaagaa gtcgtattgc ttgatgtgat cgatagacct
     9901 ttaggcactt atttagatgc tgagatgaca gatattttag aacaacacct aaatgaaaac
     9961 ggtattgaag ttttgacaaa tgcaaaaatc gaagctttta ctggtaacgg aaaagttgag
    10021 gcaatcaaaa caagtgaaaa agaaatccca gcagatctag tcattcaagc tgccggtgtg
    10081 aaggcaaata cagaatggtt aaaagggatc gtcgacttag atgaacgtgg ctggatccaa
    10141 acaaatgaat acttgcaaac gaatcttcca gatgtgtatg cagtaggaga tgctacactt
    10201 gcttattcta tcccagcacg caaaaaaatg ccgatcgctt tagcgactgt tgcacgtaga
    10261 gaagcacgtt atgtagtaaa acatcttttt gaagaagtac caaacaaacc gttcggcgga
    10321 gtcgtaggtt cctctgcatt aagtgtcttt gactatcatt ttgcagcaag cggattgaat
    10381 agctttacag cagcaaaagc aggagtagat gtacgagtgt cttattatga agatacgtta
    10441 cgaccaaaat atgtaccaga agaacaagga aatccaaaag tgtccatcca attattctcg
    10501 gatccaatga cccatcaatt gctaggagga gcagtattat ctacctatga tgttacaggt
    10561 caaggaaatg tattggcact agcgatccaa aacaaactga ctttagaaga actagcagaa
    10621 gctgacttct tcttccagcc aggctttgac cgacaatgga gcatgttgaa tctagccgct
    10681 caacaagcgt taggagaaga accatttgta gaataagaaa acgaaatatt tgaaaagagg
    10741 ttgtgaaaaa gctgccttgc atcaagtaat aagaacagcc aagtaaaaac agcttcttat
    10801 gtttttaact tggctggact tattaccgca gatgcaagct tttgaacacc gtttattttc
    10861 aaaaaaggga ctgtgacacg atttgtgtac cgacccccaa aagttagacc aaaaaatcta
    10921 acttttggag gtcggtattt tttatggtta aatatacttt tgaacttaaa ttaaagatcg
    10981 ttcatgatta tttggatggc aaaggcgatt ggaccgtaaa aatccagatc aagagattga
    11041 agcggaaatg cttaaaattc gggagaagca caaggattat ggctgtttac ggatgaccaa
    11101 tgaactaaga aaccgcggtt tctctatcaa taaaaagaaa gttcagcgct tgatcaagaa
    11161 gctaggaata aaagtaacgg catacacgcg taaatcacgt cgctacaatt cttatcgagg
    11221 aaaagt
//
LOCUS       DAYYCW010000091        10878 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000091 DAYYCW010000000
VERSION     DAYYCW010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.093"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..225
                     /locus_tag="ACHAYA_002241"
     CDS             13..225
                     /locus_tag="ACHAYA_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606024.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(258..452)
                     /locus_tag="ACHAYA_002242"
     CDS             complement(258..452)
                     /locus_tag="ACHAYA_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606025.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(465..647)
                     /locus_tag="ACHAYA_002243"
     CDS             complement(465..647)
                     /locus_tag="ACHAYA_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606026.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(644..784)
                     /locus_tag="ACHAYA_002244"
     CDS             complement(644..784)
                     /locus_tag="ACHAYA_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606027.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(1274..1639)
                     /locus_tag="ACHAYA_002245"
     CDS             complement(1274..1639)
                     /locus_tag="ACHAYA_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606028.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(1679..1981)
                     /locus_tag="ACHAYA_002246"
     CDS             complement(1679..1981)
                     /locus_tag="ACHAYA_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8606029.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(1981..2280)
                     /locus_tag="ACHAYA_002247"
     CDS             complement(1981..2280)
                     /locus_tag="ACHAYA_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606030.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(2273..2437)
                     /locus_tag="ACHAYA_002248"
     CDS             complement(2273..2437)
                     /locus_tag="ACHAYA_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606031.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(2443..2712)
                     /locus_tag="ACHAYA_002249"
     CDS             complement(2443..2712)
                     /locus_tag="ACHAYA_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606032.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(2724..3473)
                     /locus_tag="ACHAYA_002250"
     CDS             complement(2724..3473)
                     /locus_tag="ACHAYA_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8606033.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(3470..4162)
                     /locus_tag="ACHAYA_002251"
     CDS             complement(3470..4162)
                     /locus_tag="ACHAYA_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8606034.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(4168..4839)
                     /locus_tag="ACHAYA_002252"
     CDS             complement(4168..4839)
                     /locus_tag="ACHAYA_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8606035.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(4832..5173)
                     /locus_tag="ACHAYA_002253"
     CDS             complement(4832..5173)
                     /locus_tag="ACHAYA_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606036.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(5351..6049)
                     /locus_tag="ACHAYA_002254"
     CDS             complement(5351..6049)
                     /locus_tag="ACHAYA_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM8606037.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            6136..6606
                     /locus_tag="ACHAYA_002255"
     CDS             6136..6606
                     /locus_tag="ACHAYA_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606038.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(6649..6828)
                     /locus_tag="ACHAYA_002256"
     CDS             complement(6649..6828)
                     /locus_tag="ACHAYA_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606039.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(6815..7153)
                     /locus_tag="ACHAYA_002257"
     CDS             complement(6815..7153)
                     /locus_tag="ACHAYA_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFM8606040.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(7169..7372)
                     /locus_tag="ACHAYA_002258"
     CDS             complement(7169..7372)
                     /locus_tag="ACHAYA_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8606041.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(7387..8163)
                     /locus_tag="ACHAYA_002259"
     CDS             complement(7387..8163)
                     /locus_tag="ACHAYA_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM8606042.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            8462..8782
                     /locus_tag="ACHAYA_002260"
     CDS             8462..8782
                     /locus_tag="ACHAYA_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8606043.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            8800..9231
                     /locus_tag="ACHAYA_002261"
     CDS             8800..9231
                     /locus_tag="ACHAYA_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8606044.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            9361..9822
                     /locus_tag="ACHAYA_002262"
     CDS             9361..9822
                     /locus_tag="ACHAYA_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFM8606045.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            9856..10491
                     /locus_tag="ACHAYA_002263"
     CDS             9856..10491
                     /locus_tag="ACHAYA_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFM8606046.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            10604..>10878
                     /locus_tag="ACHAYA_002264"
     CDS             10604..>10878
                     /locus_tag="ACHAYA_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606047.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVE"
BASE COUNT         3258 a         2065 c         1709 g         3846 t
ORIGIN      
        1 aggtgattta tcatgaatat caacaattta gtaaaactcg ttgaacaacg ttttaaagaa
       61 tcaggattag aacaattaaa aaaagataat tctccagaat tattttctaa ttttaccgaa
      121 gacgacattc ctactcttaa acaattaaaa tattattctg attattggac gcaaactcgt
      181 gagaatgaat taaatttaga aaaactcatt attaaaaaga aatagcaact ccagcccaca
      241 cggctggttt tttagtttca acttccctcc ttacgggtca aaatcatcgg ttccggcata
      301 atctgatcat cctttagcaa caaatcagga tattttttcc ttagcttctt attgttcgtg
      361 taagttttca atagccattg gttggcttcg cttttgcttt ctccagaaaa ataaattttc
      421 cctgtttgga tgcctgcaag ttgaatcatc attttcacct caaatcaaat tttttctgct
      481 agatggagca aaagcaatcc gacatcaaga atcttttgac ccattatttc agctactttg
      541 ctcggcttaa ctccttgaga aaagagcaag cgtgcttgct ctatctcttc gtgagtccaa
      601 ataaatttgg cttcctcaag gataattact ggatcagttg ctctcattgt tcctctggaa
      661 aaatgatgtc attcttatgc ttggaccagg tatctgcata tggaacaaag tattgtctag
      721 ctaattcgat ttgatcaatt aaagcagttt ctgataattc atggtctgca gcaatttcat
      781 tcatactatc gccttcatct aaacagataa gaccgttgac gttatcgatt tttacatttg
      841 tgactgcttg actttctcca cgctcataat attctacagt tgatactgat acatcagctt
      901 cggagtttaa ttgcagaatc tgttactgcc gggataattt aatattcttt tcataattca
      961 ccttttttcc tactgacatc gaatattttt catgttatga ttagccgtga aaggagagct
     1021 atattatgtg tttacacatg atgtccttag ctcattcggt tctttggaag cttttttgga
     1081 aataactctc aggttcttga cgcaaatctc atttattctt tgaaacaaaa aacttccatt
     1141 ttccgagagc tttggatcac ttcaaaagag atttgcattt atatttctgt gaccttgagt
     1201 cgcgtaaaga accttacagc aatggcggca attccacttt caggaaaccg ccttttttca
     1261 ttgatattgg tggttagcgg actacccatt ttctttcggc agtgccaaac cacaaatgat
     1321 ctgtgttgat gtttaggtag ttgtatacca gataagctaa atccaaagtg cctgcatctt
     1381 caagacgaca atattccaaa aattctccca tagctggaca taaaaatcct ggagctatca
     1441 tatcccaaca gttaatagtg caaagaaatt cgtttagctt ctgaacgttt tctttggttt
     1501 cacgaattac aacatactcc aattcttctc cgctggcaga gcattccaaa acttcaaatt
     1561 gattaagaaa tggtagaaca tatgcttcaa acatcaacat tccatctttc tgtttttgca
     1621 cttgttttgc atcaatcatg tctatcctcc tattcagtta tttcttccat taatggaatt
     1681 atttcaggtc actggactta acgaaaacac cattgaccat ctttccagtc cgacctttga
     1741 tttcgtcata agcaaaatcc aagcactcgt ataaatccat atcattttgc attgccaaga
     1801 tgatcaaggt tactactaca tcgccaattc cgtctcttaa accatgttca tcttttctag
     1861 ctagagaagc ggcaacttcc ccgatttctt cgaccgtttt taacatttgc ttgctgaaat
     1921 ccgcttgatc caatccctta tcttttgccc actgctctac tttcgcgatt agttcgtcca
     1981 ttatttctcc tccacatacc taaactgtct tccttttgaa tcaatccata agctcctagc
     2041 tctatcccaa ataatatttt tgctcagacc agtaatttca gataactgtt tagcggtacc
     2101 cgttactaga attcgatcac catgccagat tgcaatcttt ctcggcgttc tccgtttggg
     2161 cttttcagtc cacattgatt taccgagctt ttggacttct gcaactattt ttttatcttc
     2221 ctgccaatct tctgaataag tcaattcgat aattcgctgc attgccgctt tcttatccat
     2281 cccgacattc tcctttcagt aatttgagta cttgatcaag tgcgctctca cgtccgccat
     2341 gaaatgtgtt gagccacttg tcttcgtacg acacactttg tcttaaagct tcttgatgca
     2401 ttaattcgat ctgtgctgta aatgtcttta ggtccatctt gtttacacct gctcaagttc
     2461 attaagatgt ttttgcaagc ctttaacaca atcaatgaat agtaatttgg tatatgctaa
     2521 atttcttaat tgcgttccat cgatatagag tgcgaaatag tatctgagtt tactccaact
     2581 cgaacgatca ttcttaattc cttcaattcc agcttcttcg agttgatcat atacgtctct
     2641 caggacgtct aattcctcgc cctttttata attcgctatt tcattaatta gttctagata
     2701 atcgatcttc atttttccac ctcttagaat ggtgcttttg attgtctatt agctcgttct
     2761 agcgcttttt tcttgagata ggcttcttgg tcgattgccc attcaggaag cttctctcgt
     2821 cttcctgtgc gcttgtatcc actgcttgcg ttcttaggtt cacttttttc tttccttgcc
     2881 caacttcgaa tagttgccaa atagttttta taagtcttac cagatgattc acaatactct
     2941 gacagtcgtt cgattcgctc ttggtaatca ttagggaatt ctgttttgag tttctccatc
     3001 tgctcatctg acaaaagaac atttttatac tctccgtatt tatgacggac gggcttagcc
     3061 ttcgattttt tcgaaggcgg taactctctt atatattctt ttgtattatt aaatgtatta
     3121 ttaatagatg tattattatc tttgactttt tcgtcaatag gggtattgcg tttttcgtca
     3181 atagggtatt gattaattcg taggtaccta ttgattattt gattggtacc ctctttgtaa
     3241 atgatttccc gattcaagta tccaaactta atcaaatcac ttacccatcg cgatatggtc
     3301 tctttattca caccatataa atctgcaaag tactcattgc ctgcccaaca aaagcctctt
     3361 tcattacaca aggccgttat ctctccgtat aacaacttag tatttggttt aagtcttttg
     3421 tcgtacctta cgttggctgg tataatcgca taataacttc gatgttctgt catttttccc
     3481 ctccaatatt taactttttg attgttttct ggtttaattt gatccctttg atttgatatt
     3541 tatttttgaa attaatcaca cctattttgt gcttctctgt gtgatggatt ctgcagagtg
     3601 ctgcaaatgt gtactctgca tgatcaactt ctttgcgctt tcgtcttcct agcgctttgt
     3661 caaagtgatc gatgtcagct cctgttttgc cacagatgca gcagactctt tttgtgatgc
     3721 atttgtagaa gtaatattct tgattcgcag gtaaaatctc atagccttct ttgaaaggaa
     3781 tatgatgttc aaagatgaaa tctaagatga tatttgctaa gacattggca tcactcacag
     3841 ttgtattcga ttcgtctttc aggcttattt tgcgccctgt gacaccttca aaacgaaagt
     3901 agaagaattc cttccagaag tccgttggca tgcctgtatc gataaaaata tcgcctatca
     3961 gcgcatagat gaagtttcgt tgctgtacgg tgaaacgtct aggatcaata aaacgaattt
     4021 caatgactcg atcaccatcg tagccgtcat acatcgtctt tagtcgatca atgttcactt
     4081 cctcattgat ggttgcgctt atgtcttttc ctttgaactt tttcagaacc gctgaatatg
     4141 aatcgattaa tggtttaaac actcatatca cttcttatct aattcttttc tcttagctgc
     4201 tattgctcgc tccatcaagg cacattgctc atagcttaac tgttcaatag tttcaacgtt
     4261 atcagctaag agccctaatt tatctgtctg ctcattaaca tattcgatta aggttttggt
     4321 catatcttta cccatctgct cattgaaagc ttctagaatc gtctctagca tgtttaattt
     4381 ctttgtatcg attctaggtg gtgttggaat atcttcccct tgaaatacat ataatcccag
     4441 tccgtgtaga gccaatgctt tcacaaagca tcgcttcaat gagttattga tttgcattgc
     4501 atttggttta acaactggtt ggtttcgata atctaaaaca ggaaataatt cggtttccgt
     4561 gtgtccttta accgttactg agacagatac ataagtccca gtttcatcca taagaaaagg
     4621 tttatattcc tcaacaagaa agtcttgatg agttccagaa acaaccctgt agtgtttgta
     4681 ctcattaata gttaccgttg cctgtggatc attctttttc ataatctccc acgcgtgagc
     4741 ccaagataaa taatcaaaat ttcctttttt cttgagaatt ttatttaact tacgactaaa
     4801 aagtttttca aagttcgttg tccctttgat ttcactcatc aaattctgcc tccatttcag
     4861 caatgtattt cttacctggt ccgtaataag agatatcaat caagttatct ctgtcgtact
     4921 cttctagcgc atcaatcaag ccatcttcga tgacgtaaat atattcaggt ttattcgaat
     4981 gcttcgatag atggataaga taaacatgat cccaaatagt tacatggttg cctaaatcat
     5041 cttgatccca agctagttct tcattcgtca aaagatttcg tctgattttt cgaccacttg
     5101 tttcctcaat tttcggcttg ccccaatcag gatcaatcaa atattgatct agagtggaaa
     5161 gttctttttc catatgctaa aatctcctta tgatgtgttt tctttgtgac tctttgcttg
     5221 ccggcggagt cactttttta tttgttgcca tgctttttgc ttgtcaatat gttgttggct
     5281 taggatgatt ggtttatagt atttccacca gcaattagca attgccgtcc ctattcttag
     5341 cgcttcagct ctattcattg tcatcaccaa aaagtctctg ttgtctgttc agttgatcaa
     5401 tttccatacg gatcgcagtt tctggcaacc acatttcaat aaatgaaaca gcatcatcga
     5461 atctcttacg aggtaactcg ccatatcttg ggattgaaaa ggtacgttta aattcagacc
     5521 aaaattttga gaatactttt ttgctgattt cttcataagc tcggctttct tttcccccta
     5581 gaactcccat aactttcata tttccttttt gcttaatttc aaactcttgt tgtccgctaa
     5641 ttcgcatagt atctttaagc atggaaacat cttttttaac atctttcatt tcttctaatt
     5701 gatagatcat catatcttca attgtttgag gaacagtatt cttgcgaata acatcttcca
     5761 tttcgttgaa tgcttcaatg tatttttgtt tgaagtagat agctttcttt cctgtaaacc
     5821 ccatagccag caagaaaaat ccatctctac taatgaagaa aactcgtcga tttctgccat
     5881 atgaatctgg ttcattacct tctacaaaca tctgtccaaa attggacaca tcttttttta
     5941 gtgcatcaat atctcttaaa acatgttgat gttttttctc gaagctttct gccacttgta
     6001 agctcgtagt cacagcttct ttatttttca aaattactaa ttcttgcatt atttcttctc
     6061 tcctttttgg tataatttag ttaaaaactg gtggtgttta ttttgaattt ttttcactta
     6121 acaatttttc aatggatgtc aataataagt tttatattga gtacgttttt aacagggata
     6181 aaaataaaag gaatgcgccc tcaactagaa gttgaactta atgcttctta cttcgctgca
     6241 gatatgattt atacaaaagt aatcatttct aactattcaa cagaaccagc tatgttagtt
     6301 aatttagaac tttcatccgc taacttaaac cgcagatggt cagctactcc atttaagaaa
     6361 ttaattgcta aaggaggatc agtcgatgat aatagaatat actctgaagc tgtaccgcta
     6421 aatattcctc ctaaaagtgc tatatcttgt taccttgcat ttgaagtagg gaaaattaat
     6481 tttaataaac tccttaatca ccaaacaaaa atgatattta ctctaaatag aacccaaatc
     6541 cacaaaattg ttgatatcaa aaatacaaac ttcccaatag agaaattagt gaaagaatta
     6601 aactgagtaa tagtaatata cattctagtt tctcttttct attcattttt agtcagcccc
     6661 ctcggttggc tttttcgctc tgtactcagc ttcagcttca tcaagcccca taaaaatcca
     6721 aaccatgtaa acaatcgttc ctatcaacgc ttgcctgctt ccccaaagtc ctaaagcgta
     6781 gatgattagc ggtgcgctga acactagcgc tctattaaat tttcccatcc gcttgcctcc
     6841 ttaagatttc cgaaaaattt gtttctaaaa attcaagtgt tttacttctt aaaaatagat
     6901 atgtgtctct tccttcaact ggataataga caaatccatt tttgtttttc tcgatatcga
     6961 taatatttct atatcttggg ttctttaaaa ctctagaagt aaaccaatca tattttctgt
     7021 taatccgttc tagcacttct ggcaacgtca tccatctacc agtatcatca gcttttttta
     7081 actcctcata atccacttgg gagataatta catagccttc tggaattggg atttttgctt
     7141 ctagatattg cattagctgt tcctcccttt acaattcgta catagtgata atcgaatcta
     7201 taattctgtt tgcttctgca gaagtctttt taccgtttaa aattaaagat aagtagcttt
     7261 tgctaattcc aaatctttca gcaagcatgg tgtaagttaa gaactttgaa ctttcgacat
     7321 attctttgat tttttctcta tctcgttgag tgatttctgc aatgtcagtc atactaaaac
     7381 tcctttctaa ccaatttcct ctaaatccat ttgagggtaa tatccttctt tttttagtaa
     7441 ttcgtagata aatagacgcc ctttctgtgt ccatttggta ttcattacaa ttttagtgcc
     7501 accatcggct ttcgggatct cagttgtatg agattttgtg tatccttggt tcatgtgttt
     7561 tttgcacaat aaccattggt taccgacttt tttctgaata cctagtttat gaagtaattt
     7621 attcatctgt tgtggagaca tcccataatc tgctgcaatc tgactaattg ttactgaatc
     7681 tgtagaagat aatatgctat ctaaatagga gattttaggt tcgtactctg caatcttttg
     7741 ttctgcgatt aatcttccag ttctttcttc ttttagttga gttgctaatt gaatgattgt
     7801 atctggatta agcaaagctt cttctacttt ttctggagtt agataacctc catgttttct
     7861 aattgctggc aacacttcac ttgttaccca acgtttaaat tttttggcag agggaagttt
     7921 tgattttaag attaaactgt acaaaccaga ttcgttgatg attgtcatct ctcttgattg
     7981 acctgaggtc gtgattcgcg accccatctt atcttccaaa tctacatgcc gcgacaaagc
     8041 atctttagtg tttgaataac ccaaaacact cgcaacgtct ttgcctacaa aatatggttc
     8101 atcatttact aaaattgtcc gaacttcgtt ttgttcgaaa ttaaaaattt gcggtgtgtt
     8161 catatttctc attcctttct tttatattcg taaacaaatt taacaacttt tttctaaatt
     8221 caattgacag tatctgagta atactctata ctaagagcat aattaaataa gacataaaaa
     8281 cattgatttt aaagctttct tggcggtctg catttacata tcaatagtgt tttttgttgt
     8341 ctttttagtt gttaaatctg tttacaagat atagtatagc tcattactca gaattagtca
     8401 aattatttct gcgtattttt ctaaactttt tttgtaaaca tttagaaagg ttgttttatc
     8461 gatgaatacc tatgaaataa taaaagactt ggcgaagagg aaaaagatat ctatacggca
     8521 gttagaaatt aattttggat attctaacgg ttatctagca aagtggaaaa ataatactcc
     8581 taattcagat gaattaagtc ggattgctga ctatttcgat gtctctgtag actaccttct
     8641 aggtagagaa gaaagagaaa cccctaaaca tgtggattta tcagaagacg atactgtttt
     8701 ttcttttgat ggaaaagaaa tatctaagga gacaatgcgt aaagcgattg caattgctaa
     8761 agctttagag gaaaatgaat agttggagtg atggtttgta tgtatttgaa attgaaagaa
     8821 atgttgagcg aatacaattt gaaagtagtt tatatggaga tgaaagagcc tggtttctac
     8881 tatccaaaac ccagaatcat ttttttgaat gaaaatctat acggtgaaac tgcagaagcc
     8941 tttcatttat ctcatgaact tgcacatttc agcgcctctc attttgaatt ttcagttttg
     9001 tacgatactt ctacaacttt tcactcaaaa tttgaaacag aagcagataa aatagctatt
     9061 ctaattttat taaatatcta tattgagaac gaactgactg atgaatctca gtttaatctc
     9121 gaaaaattta tggagtatta ttctattcag aataagctca gatacacttg ttatgctgtc
     9181 tgccaatgct attttaagaa aaaatattct tacgcaaggc aatatgtatg aatacatcaa
     9241 gaattattaa aaaaaccgat ttcagcaaaa cattaaaaaa gcccgtgctg caacacgggc
     9301 tctttcctca tatatgagct tctacaaaaa aaatcatatc acagaaatga ggaagaaaaa
     9361 atggaaaaag agattttaat aaaatctcca ggaaaaacct tgattaaagt aactgatgat
     9421 tctatttcaa ttattagaaa aggatttata aatttagtta atcaaggtat taaaggagaa
     9481 aaaactatcc cctttaaaaa tatatcagcc gtacaattaa aaaaaccagg tatgagtaat
     9541 ggttatatac aatttacctt gctaggcgga aacgaaagtc gcggaggtat attagcggcc
     9601 acgaaagatg aaaacaccgt tatgtttaca aaaaaatatt ggaatgaaat ggaaaattta
     9661 aaaaaatata tagagaagca acaatctata ttagataata attccaaaga aacacaaatt
     9721 tcaagtgcag atgaaattaa aaagtataaa gagttgttag atgaagggat tattaatcaa
     9781 gaagaattcg attttaagaa aaaagaattg cttggtttat aatattcaat atcaaaagag
     9841 aaagaggatc tattaatgga cggtatattt ggttttactg gtattgtttt atttattata
     9901 ggtttaatca tgttaattgt aagatttatc aaaaaaacaa ataagaaaac acctatgatt
     9961 ctccttatta tcgggattat ttttactacc gtaggtttct cgctttcctc atcaaacgag
    10021 gcaaatgaca ataacaaaca ggaaagctca tcatctgata ctcaaactac tacatctaac
    10081 agcagcaata aaagcctaac tcaatttgaa gaatttttag agacaaataa tcatgactgg
    10141 ggtgctttct tagatagtta ttattctatt actccagcta ctgagcaaaa tacagctttt
    10201 actaaatata ttagtggtaa aacatatact tttgaaggaa ctgttatcga atcaatgact
    10261 actagaatag ccattatagc agacaaagag tacgatgata aaagttggaa tgatatttct
    10321 gcaacaccta aagtttcata tgtaattttt gcgaaggact taaacaatgc tgataccttt
    10381 acaaagggcg ataaagtaac ttttacagga gaaattagtt caagaggatc gaacatagaa
    10441 aaatcctatg ctcaatggga tatgattaat agtaaagtaa gtaaaaaata aatgaaccac
    10501 gccccactaa gagttagatt gtgcacaata ataaaaccta tacaaaagag tgccatcagg
    10561 agtcacaatg cttgtaaagg aaatgacaaa ataggaggta actatggata atagcaatga
    10621 aaaactaacc aaaaaagaat atcttgaatt aatagttgaa ggcggtattg gagcaattcc
    10681 ttatattggt ggcgttgtgc agtcacttta ttttgggtct aaaaacgaga agcgtttcaa
    10741 acgcatcgag aaattctaca aaaatttaaa cgaatcatta gaaaacattc gcgatcaaat
    10801 tccagaagat gttttcaact tagagaacaa ggaccaactc attggaatat tcgaagggat
    10861 aaatgacgag gtagaaaa
//
LOCUS       DAYYCW010000092        10777 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000092 DAYYCW010000000
VERSION     DAYYCW010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.094"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACHAYA_002265"
     tRNA            12..84
                     /locus_tag="ACHAYA_002265"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACHAYA_002266"
     tRNA            88..160
                     /locus_tag="ACHAYA_002266"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACHAYA_002267"
     tRNA            168..249
                     /locus_tag="ACHAYA_002267"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACHAYA_002268"
     tRNA            267..339
                     /locus_tag="ACHAYA_002268"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACHAYA_002269"
     tRNA            351..422
                     /locus_tag="ACHAYA_002269"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACHAYA_002270"
     tRNA            439..524
                     /locus_tag="ACHAYA_002270"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACHAYA_002271"
     tRNA            535..608
                     /locus_tag="ACHAYA_002271"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACHAYA_002272"
     tRNA            618..691
                     /locus_tag="ACHAYA_002272"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACHAYA_002273"
     tRNA            711..783
                     /locus_tag="ACHAYA_002273"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACHAYA_002274"
     tRNA            811..884
                     /locus_tag="ACHAYA_002274"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACHAYA_002275"
     tRNA            898..971
                     /locus_tag="ACHAYA_002275"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACHAYA_002276"
     tRNA            995..1084
                     /locus_tag="ACHAYA_002276"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACHAYA_002277"
     tRNA            1097..1170
                     /locus_tag="ACHAYA_002277"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACHAYA_002278"
     tRNA            1189..1264
                     /locus_tag="ACHAYA_002278"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACHAYA_002279"
     tRNA            1266..1336
                     /locus_tag="ACHAYA_002279"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACHAYA_002280"
     tRNA            1350..1423
                     /locus_tag="ACHAYA_002280"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACHAYA_002281"
     tRNA            1448..1535
                     /locus_tag="ACHAYA_002281"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACHAYA_002282"
     tRNA            1557..1628
                     /locus_tag="ACHAYA_002282"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACHAYA_002283"
     tRNA            1651..1740
                     /locus_tag="ACHAYA_002283"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACHAYA_002284"
     tRNA            1753..1826
                     /locus_tag="ACHAYA_002284"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACHAYA_002285"
     tRNA            1845..1917
                     /locus_tag="ACHAYA_002285"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACHAYA_002286"
     tRNA            1921..1993
                     /locus_tag="ACHAYA_002286"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACHAYA_002287"
     tRNA            1998..2068
                     /locus_tag="ACHAYA_002287"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACHAYA_002288"
     tRNA            2081..2154
                     /locus_tag="ACHAYA_002288"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACHAYA_002289"
     tRNA            2166..2237
                     /locus_tag="ACHAYA_002289"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACHAYA_002290"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACHAYA_002290"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM8606048.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACHAYA_002291"
     CDS             3826..5298
                     /locus_tag="ACHAYA_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8606049.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACHAYA_002292"
     CDS             5381..6169
                     /locus_tag="ACHAYA_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8606050.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACHAYA_002293"
     CDS             complement(6236..6904)
                     /locus_tag="ACHAYA_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM8606051.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACHAYA_002294"
     CDS             7019..8731
                     /locus_tag="ACHAYA_002294"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8606052.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="ACHAYA_002295"
     CDS             8734..10506
                     /locus_tag="ACHAYA_002295"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8606053.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(10588..>10777)
                     /locus_tag="ACHAYA_002296"
     CDS             complement(10588..>10777)
                     /locus_tag="ACHAYA_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8606054.1"
                     /translation="KKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTH
                     TTKNMQNQLVEKLGKIEI"
BASE COUNT         3092 a         1951 c         2488 g         3246 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag
    10621 ttgattttgc atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc
    10681 atgtcctaca cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat
    10741 gtgggaatgc cttaacatgt gagaagaaac tttttta
//
LOCUS       DAYYCW010000093        10740 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000093 DAYYCW010000000
VERSION     DAYYCW010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.095"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            151..966
                     /gene="thiD"
                     /locus_tag="ACHAYA_002297"
     CDS             151..966
                     /gene="thiD"
                     /locus_tag="ACHAYA_002297"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM8606055.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1295..2746)
                     /gene="cls"
                     /locus_tag="ACHAYA_002298"
     CDS             complement(1295..2746)
                     /gene="cls"
                     /locus_tag="ACHAYA_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8606056.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3087..5753
                     /locus_tag="ACHAYA_002299"
     CDS             3087..5753
                     /locus_tag="ACHAYA_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8606057.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6117..6869
                     /locus_tag="ACHAYA_002300"
     CDS             6117..6869
                     /locus_tag="ACHAYA_002300"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8606058.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(6891..8045)
                     /locus_tag="ACHAYA_002301"
     CDS             complement(6891..8045)
                     /locus_tag="ACHAYA_002301"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM8606059.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8230..10671
                     /locus_tag="ACHAYA_002302"
     CDS             8230..10671
                     /locus_tag="ACHAYA_002302"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM8606060.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQETAIQILEKIREK"
BASE COUNT         3499 a         1910 c         2306 g         3025 t
ORIGIN      
        1 ttctgattta ttgcaaatgt tttttcaaat aaatttgatt accaagaaaa ggtagtggta
       61 tatttactga gggatacttt gaaaaacata tgatagaaac agtaacagta ttatgaaaaa
      121 tctttttata aaaatggagg aatcgatttg atggaagatc agtatgtaca agcaatgacg
      181 atcgcaggat cagatagcga tggaagcgca gggatgcaag cagatatgca tacattcttt
      241 acaagaaatg tttacggtgt ttctgtcatg accgcatgtg ttgcaggtaa ttcttacgga
      301 atcggagcaa gtgtgacatt gccaacagat tttatcgaca aagaatttga actgattgcg
      361 aaggactttc aaatacgtgc agctaaaaca ggtatgctag cagattcaaa gttgattgag
      421 acagttgtaa aaaattacca aaaatatgat tttggaccat tagtcgtgga tcccgtaatc
      481 gtaacgaaac atggaaatct tttactagaa gaatctgctt tgcaaagctt gaaagaaaag
      541 cttgtccctt tggccgaagt actgacacct aatttttatg aagcagaaaa gctgactgga
      601 cggacattag cagctgaaga agattttgtg caagcagcaa aagaactgca agatatgggt
      661 gcaaaaaacg ttatgatcaa aggacatcat gctaaaaatg cgcagcaaga aacagtcaat
      721 gactacgtat tgttcgaatc aggaaaatca ttttggttaa atgcaccatt ttatcctacc
      781 gaccggatca acggaactgg cgattcgcta tctgcatgta ttgcagctga gttagcgaaa
      841 ggaaaaacag ttgaacaagc gatccgattg gcaaaagact atgtaaaccg tgcaattcgt
      901 gaagaaataa atgtagggca caaatatggc cccatcaatc attgggcagc tggtgaatta
      961 gagtaaaaga aagaagttgt gaaaaagttg ccctgcatca agtaataaga acagccaagt
     1021 aaaaacagct tcttatgttt ttaacttggt tggacttatt accgcagatg caagcttttg
     1081 aacaccgttt aagaaagagg tcgtgagcct gccgtccagc tccgagtcac aaggaacaag
     1141 ttcaaaaaac agcttctcat gtttgttgaa tattgtgact tgtgaccgag gagttggcag
     1201 gagaacaccg tttgttcgga agaagggact gtgacaagat ttttgtcaca gtccctttaa
     1261 tcttagtata tttttctcta cttgaataaa aaaattatag gattggagaa aataatcgag
     1321 aaatttgttg cttgaataat agccagttag acatttcctt gactgtttca gttgtcatgg
     1381 atgaacaatg tgtctcatct tctttgaact gagtagcaag ttcttctaaa aactcaggat
     1441 cgtaaatgac agcacttgtt tcaaaattca atcggtagct acgaatatct tgatttgctg
     1501 aacctaccat gcagatttca tcatccatta tcaatgtttt tgcatggagg aagccgttct
     1561 catagacaag gatttgtaca ttttccttga tcagctgccg agcataatat tgtgttgctc
     1621 gataaataaa aggatgatcc ggtttgttgg gaatcatgat tttcacatct acaccagagg
     1681 cagttgcgat ttttaaagca gcaatgacac tttcatcagg aacaaggtaa ggtgtctgta
     1741 tccaaacacg ttttttagca gaagtaatca atttgataaa tgaaagcttg atttgttccc
     1801 tttcgttatt cggtccgctt gaaacaagct ggatggatgt atcagcaagc ttagattcat
     1861 ctcgatctgc ttttttaaag aaataattca aatgatagcc gacctttttt tcttcaggta
     1921 ccgagacgtt ccagtctgtt aagaagcgaa gctggagcaa agaagaagcg gcaccgaaaa
     1981 tccgtatatg cgtatcgcgc caatagccga atttttttgt tgtatttaca tattgattgg
     2041 caatattgaa accaccggta taactaatct ttccatcgat cacaacgatt tttcggtgat
     2101 catggtaatt caatcggaaa cgaagtaatg ccctttgtga ggtaacaaat gtatggacat
     2161 gtccaccatt tttgattaga cgattgaaat cttttgcttt tgtgccatga gagccaaatg
     2221 catcatataa tattcgaact tccacgcctt ctgcggcttt ttcttctaaa acatgtaaga
     2281 cttgctggcc gatattatct gtcacaaatg cgtaatattc gatatggatc gaatgtttgg
     2341 ctttttttag atcttgaagc agtgaatcca atttctcttg tccgtctgtg agaagagtga
     2401 cagaattcat tcttgtcagc ggcatacgat ttaatgaaga gaagaagtca acaaattgct
     2461 gtttgtcttt atcgcccatg tcaggatcgt aatgttcaat ggtatctcct ctaaaagact
     2521 gaaagttttc taattctttc aagtcacttt gtcggagata gaattttttc ttatccgtta
     2581 atccacgtcc gaaaaataaa tataatacaa agcccacccc aggaagggca aacaatacta
     2641 agagccatgc ccaaatggct gctacatctc ggggtttgat caaaatagcc accaaagcaa
     2701 taagtgcatt taatagataa agggcggtta taatactaga taccacaaaa atgttcctcc
     2761 ttacatacaa taacattagt atagcatgat agaagaaaga agcctaccaa ggtgaatata
     2821 cttttcactc ctttcatgaa aatgtaggta aatcgggcta aagtagttgc tttttgtagg
     2881 ttaataaggt aggatattag tacagttgaa tatctcctta ggggagtaac cggcaacgtt
     2941 tgttgcgata atatcaacag taagttaacc aaatggtttt ctggtattgt atgaaatggt
     3001 gagacctaag acaaatgcgt atttgtctta ggtctctttt ttttcagaca ggactagggc
     3061 caagatcaaa aaatggggga tgaaatttgg aaaacaagga acaagcacaa caagcggtct
     3121 ataaagaaac gatcgactcg ttgatgcagc aaacgcagag ctcaaccgat ggattgagca
     3181 ccacgcaagc gaaggaaaaa ttagaaagag atggcttgaa ccagctaaaa gaaaagccga
     3241 agaagccaac ttggaaattg ttcttagaaa ctttcaaaga tgcaatggtt atcgtattgc
     3301 tgattgtcgc actgattcag atggtcatgg gtgactacat cgaattctta gttatttttg
     3361 cagtattgat gttgaattct ataatcagtg ttgtccaaac aaaaaaagca gaaggttctt
     3421 tagaagcgtt gaaaaacctt tcagcgcccg atgctcgagt gattcgtgat ggtaaggaaa
     3481 aaacgattcc ggcaaacgaa cttgtggtag gagatatcgt gttgttagac gcaggcgatt
     3541 atgtacctgc tgatggacgt ttactagatg cgggatcatt aaaagtagat gaaggtatgc
     3601 tgacaggaga gtcgacttct gcagaaaaag aagtaacgga tatcacaagt acggtaccaa
     3661 tcggagatcg tacaaatatg gtattcagtg gaacgatcgt aacttacgga cgcggtaaat
     3721 tcttagtgac ttcaactggg aataatacgg agatcggaca agttgctggc cttttggaat
     3781 caacaactga gcaagtcact ccgttacaac gaggtctgga taaattcagt aaaaaactaa
     3841 gtttggctat tttggcattg tctttattga ttttaggtat ccaactgttt cgtatttatc
     3901 ttggagaagg tacaggagat atgactgcag atatcgtcag cgcagtgatg tttgccgtag
     3961 ccgtagccgt agctgctatt ccagaagcat tacagtctat cgtaacaatc gtgttatctt
     4021 tgggaacgaa taaaatggca aaacgtcatg ccattattcg gaagcttcca gcagttgaaa
     4081 cacttggttc gacaagtatc atctgtacag ataagactgg tacattgaca caaaacaaaa
     4141 tgacagttgt tgactacttt ttaggtaatg gcgatactgg ggatttcaca aatgaccctt
     4201 caaaatggac agctgacgaa cgtcgcttga tcgaaatcag tgttttagcg aatgatgcat
     4261 cgatcagtga agacggtact aaattgggcg accctacgga agtagcgttt atcgacttta
     4321 gtgaaaaatt aaaccagcct tatcaggaaa tccgaaacaa ctacccaaga caagcagaac
     4381 ttccttttga ctcagatcga aaattaatgt ctactggaca cacaattgat aacaaaaatt
     4441 acttgtttgt taaaggcgga ccagatatcg tcttccaacg cagtaaaaaa gtattgctaa
     4501 atggtgaagt cgttccgatg acggatgaat tgatgaaaaa attccaagag caaaatgaag
     4561 ctttttcaac acgcgcatta cgtgtactag cttttgctta ccgtccactt gataattttg
     4621 atttatcatt tgaagatgaa gacgatttga tccttgttgg tttgcttgct atgatcgatc
     4681 ctccacgtgc tgaagtgact caggcggtaa cagaagcaag aagtgcagga atcaaaacaa
     4741 tcatgattac tggtgaccac aaaacaacag ctgttgctat cgcaaaacaa atcgggattt
     4801 ttgaagaagg aaacctggct ttgaccggga cggaactaga tgaattgaca gaagatcaac
     4861 taaacgaagt tttagaaaaa gtcactgttt atgcacgtgt ttcacctgag aataaaattc
     4921 gtatcgttcg tgcttggcaa aataaaggtc atatctcagc tatgacaggt gatggtgtaa
     4981 atgacgcacc agcattgaaa caagcagaca tcggaattgc tatgggaacg ggtacagatg
     5041 tggcaaaaga tgcagcagcg atggtattga cggatgataa ctttgcttct attgtcagcg
     5101 cagtagaagt aggacgtaat gtgtttgata atatcaagaa agctatttct tatctatttg
     5161 caggaaattt aggtgcgatc attgcgatca tctttgcctt agttgctgac tggtccaatc
     5221 catttacagc attacaactg ttgttcatca atttagtcaa tgattcactg ccagcaattg
     5281 cattaggtac agaaaaagct gaacctgcta cgatgaaccg cccgccaaga gatccaaatt
     5341 caggaatctt tacggacaaa acattgattt ctgtaacgta tcgtggtgtg ctgattggga
     5401 ttgctgttat ctttgctcaa tggcttggca tgcagcattc acctgaattc ggtgtagcca
     5461 tgtcattttc aacattgatt tggtgtcgga cattgcaaac attcccagca cgttcaaaca
     5521 cacaaacagc tatccaagca gggttcttct ctaacagaag tgtcttacta gctgtagttg
     5581 tatgtagcgc gctttactgc ttaacaatga tcccaggact gaaagaagtc ttctctatcc
     5641 catcagcatt tggattgaca gaagtcttta catgtatggg aatcgcactt gtgacaattg
     5701 ttttgatgga aatcactaaa ttgatcattg tacgagtaca aggaaataaa taaaacgaat
     5761 caacgaggtt gtgagcctgc cgtttaattc tgagtcacaa ggtgcaattt caacaaacag
     5821 cttctcatgt ttgttgaata ttgtgacttg tgaccgaaga gttggcagga gaacaccgtt
     5881 taaggaagaa gttgtaaaat cagcattttg agctaatact gagggtcatc tgattggaca
     5941 ctgtttattc ggaaagaggg actttgacac gactttggtc acagtccctt tttttattta
     6001 tgatagacgg aaagccaatc attttattat tcatcaaaag agatagaata gataaaagag
     6061 gagaatgatt tattccgaaa agtgaaaaca tataacaaaa aattagagga aatcaaatga
     6121 atgaaaaaga atgggatgaa tttgctgaag aatattatga aatccagcaa gaatcgcaag
     6181 taactattgc tgaagatgta aaaaattatc tgaaaacgga gaaagtcctg cctgctgaaa
     6241 cagcagcaga cgttgctgga ggatctggca gatttctttc cttagcaaaa gaaattaatc
     6301 gctatgattt gatcgatttt tcttcaggaa tgctagaaaa tgcaaaaaaa gaagcagaaa
     6361 aaaatgagtt gccaaatgtt tcctttatca aacagtcgtt agaagaattt atccaatcaa
     6421 atgagacata tgaaatggtt tttacagcag caaatccagc attggtacat cttattcaat
     6481 tagaaaaatt gatgaaaaag tcaagcaaat ggtgcgtgat aatcagagta gtggaatcaa
     6541 tcgatgatgt tttttatcct atcgaaaagc aactaggaat gatagaagaa gatcctaata
     6601 catcgccagt tgtaatggat caatttgaaa catacttaat tgatgaagga ttcaaagtga
     6661 agaaaaaaga ttttacttat tttacacagg aatcaattac tgaagaattg atgaagctat
     6721 actatgacgc gtacgcaaaa gatcctgttg tgaccaggta cattcgcaaa ctattcagtg
     6781 aaaaacgaga aatccttagt acgactaagc tgacctatag actgctcctt tggtccgcag
     6841 ataaaaaaag aacctttcac gactcgtgaa gggttctttt tatgatttta ttatattttg
     6901 tagtaacgaa tatcattttc gatctttgta atcgaaaaag gtacatctga agtatattct
     6961 gcacattctc cgtgttcagg aagcgtctca ctgaaaatcg tttcatattc gtcaacagtg
     7021 atccgtgttc gagaatctag catctcttga tgagattgtg cgaagagata ttcttgatag
     7081 ttttcttcca gataacctgt aaaaaattca cttactgcac cagaaccgta gctgaataga
     7141 ccgatgcgat ctcctggctg tagtgatttc gaattttcca gtagagaagt tagccccagg
     7201 tataatgaac cagtgtaaag attaccgatt cgtcggctgt aacggatgct ttcttcatag
     7261 cgagccataa gacgttcctg attttcttcg tctgtttggt ctaatacgct ttgcaatgcc
     7321 ttttttccca tcttagtata cggaatatgg aaagcaatcg cctgataatc ttcgagtcct
     7381 cgacccgaca attctttatg tcgattccaa actttttgga atgattcgat atacgtagaa
     7441 ttagataaag gaccatcaac aacaggaaat tcgctataat ctggacgcca gaaatcatag
     7501 atatcttctg tcagaaatac gctgtcgtct tcaatcgata aaatacgcgg gttttgagtg
     7561 atcatcatcg caacagcacc gacaccttgc gtcacttcac caccgcttgc caagctgtaa
     7621 cgagcaatat cacttgctat gactagtact tttcgttctg gatgattttt gacatattct
     7681 ttcgccatgt gcaaagctgc ggtgccaccg tagcaagcct cttttacttc aaaagaacga
     7741 gcaaaagatt ggattttcag taaatggtgg ataattaccg cactcgcttt tgaatgatca
     7801 atcccggatt ctgttccgac gatgaccatg tcgattagct cacggtcttt ttctgttacg
     7861 attttgctgg cagcatttgc tcctagtgta atgatgtctt catttgcacg attgactgcc
     7921 atttggtctt gaccaatccc tatatggtat tttgcaggat catccccacg gctttctgcc
     7981 aactctgtca tatctaaata taaattagga ataaaaaagg aaagacgatc aatccctatt
     8041 ttcatttcaa cacctcttta cttatgataa aaaacttact ttactaaggt atcataaaaa
     8101 gattaaaaga aatataaaaa agagaatatt taaggattgt taaaatttaa tcttagaaaa
     8161 aaataactgg aacaagagct atttgttttt acagcaaaga atggtatatt tttgtaggag
     8221 gtggcttact tgaaagaagt cgttatgata gatgctgcaa gaacgccgat aggaaagtat
     8281 cgcggaagtt taagtccatt tactgcagtt gaattaggca cacttgtgac taaaggatta
     8341 ttagataaaa caaaactgaa aaaagataaa atcgatcaag taatatttgg aaatgtcctg
     8401 caagctggta atggtcaaaa tgttgccaga cagattgctt tgaacagcgg tctccctgta
     8461 gatgtcccag caatgacgat caatgaagtt tgcggatctg gtatgaaagc agtgatctta
     8521 gcacgccagc tgatccaatt aggtgaagca gaacttgtga tagccggcgg aacagagagt
     8581 atgtctcaag cacctatgct gaaacagtat cagtcagaaa caaatgaata tggtgaacca
     8641 atttccagta tggtcaacga cggattgact gacgcatttt caaatgcaca tatgggatta
     8701 accgcagaga atgttgcaac acaattttct gtgagcagag aagagcagga tcgctatgcc
     8761 ttgtcgtccc agttgaaagc agcacatgct gtcgaagccg gtgtattttc tgaggagatc
     8821 atcccagtca agatttctga tgaagacgtg ttatctgagg atgaagcagt tcgtggaaat
     8881 agtacattgg aaaaactggg cacgttacgt acagtattct cagaagaagg aactgtaaca
     8941 gcaggaaatg cttccccgtt gaatgacggt gcctctgtgg tgatccttgc atccaaagaa
     9001 tacgcagaaa ataataatct gccttattta gcaaccatca aagaagtagc ggaggtcggt
     9061 attgacccaa gtatcatggg aatcgctcca atcaaagcca ttcaaaaatt gacagataga
     9121 tcaggaatga atttatcaac aatcgatctt tttgagatca atgaagcatt tgcagcttct
     9181 tctatcgtag ttagccaaga actgcagtta gatgaagaaa aggtcaatat atatggagga
     9241 gcaatcgcat taggccatcc aattggtgca agcggtgcac gtatcttgac tacattagct
     9301 tacggattgc ttcgtgaaca gaagagatac ggtattgctt cattatgtat tggtggaggt
     9361 ttaggcttgg cagtcttgtt agaagcgaat atggagcaaa cacataagga tgtgcaaaaa
     9421 aaaaagtttt atcagcttac gccatctgaa cggcgcagtc aattaataga aaaaaatgta
     9481 ttgacacaag aaactgcgtt gatttttcag gaacaaacac tttctgaaga gctctccgat
     9541 cacatgatcg aaaaccaagt gagtgaagtg gaaatcccaa tggggatcgc gcagaatttt
     9601 caaatcaatg gcaagaaaaa atggattccg atggcaacag aggaaccatc tgtcattgca
     9661 gctgcaagta atggtgcaaa aatctgtgga aatatctgcg cagaaacacc tcagcggctg
     9721 atgcgagggc aaatcgttct atcaggaaaa tcggagtatc aagcagtgat caatgcagtt
     9781 aatcatcgaa aagaagagct gattctttgt gcaaatgaaa gttatccttc aatcgtgaaa
     9841 cgcgggggcg gtgttcaaga tatctctact cgggaattca tggggagttt ccatgcatac
     9901 ttatctattg attttttagt ggatgtaaaa gatgcaatgg gcgctaacat gatcaactcg
     9961 attcttgaga gtgtggcaaa caagttacgg gagtggttcc ctgaagaaga gattctattc
    10021 agtattttaa gtaattttgc tacagaatca ttggcttctg cctgttgtga aatcccattc
    10081 gaacgcttag ggagaaacaa agaaatcggt gaacaaattg ccaaaaaaat ccaacaggca
    10141 ggggaatatg cgaagcttga cccttaccgt gcagcaacgc acaacaaagg gatcatgaat
    10201 ggtatagaag ctgtagtggc agctacagga aatgatacac gtgcggttag cgcttcgatc
    10261 cacgcctacg cggcaagaaa tggattatac caaggattaa cagactggca gatcaaaggg
    10321 gataaactag ttggaaaact aacggtgcct ttggctgttg ccacagtagg cggagcttcc
    10381 aatatcctgc caaaagcaaa agcatcttta gctatgttag atattgattc agccaaggaa
    10441 ctagcacaag taattgctgc agtagggtta gctcaaaacc tggctgcttt gcgtgcatta
    10501 gttacagaag gaatccaaaa aggccacatg ggacttcaag ctcgttcatt agcgatctca
    10561 attggggcta ttggtgagga aatcgagcaa gttgcgaaaa aattacgtga agcagaaaag
    10621 atgaaccagg agacagctat acaaatattg gaaaaaatcc gggaaaaata aaaaagcaat
    10681 atctttagaa gaataaagca agatttacag ttattttatt tactgcagat cttgcttttt
//
LOCUS       DAYYCW010000094        10662 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000094 DAYYCW010000000
VERSION     DAYYCW010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.096"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..657
                     /locus_tag="ACHAYA_002303"
     CDS             34..657
                     /locus_tag="ACHAYA_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606061.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /gene="trpX"
                     /locus_tag="ACHAYA_002304"
     CDS             1107..2156
                     /gene="trpX"
                     /locus_tag="ACHAYA_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8606062.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="ACHAYA_002305"
     CDS             2153..3034
                     /locus_tag="ACHAYA_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8606063.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="ACHAYA_002306"
     CDS             3037..3807
                     /locus_tag="ACHAYA_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8606064.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="ACHAYA_002307"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="ACHAYA_002307"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM8606065.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="ACHAYA_002308"
     CDS             5118..6281
                     /locus_tag="ACHAYA_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8606066.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="ACHAYA_002309"
     CDS             6398..6808
                     /locus_tag="ACHAYA_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8606067.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="ACHAYA_002310"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="ACHAYA_002310"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8606068.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..9665
                     /locus_tag="ACHAYA_002311"
     CDS             9381..9665
                     /locus_tag="ACHAYA_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606069.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9825..10061
                     /locus_tag="ACHAYA_002312"
     CDS             9825..10061
                     /locus_tag="ACHAYA_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8606070.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10058..10456
                     /locus_tag="ACHAYA_002313"
     CDS             10058..10456
                     /locus_tag="ACHAYA_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8606071.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3725 a         1773 c         2253 g         2911 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9721 ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata
     9781 atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa
     9841 agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag
     9901 aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg
     9961 tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga
    10021 atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga
    10081 aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt
    10141 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
    10201 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
    10261 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
    10321 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
    10381 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
    10441 aaccgcaata aaatgaacga attaaaaact ttatttctca attagatcag catgtcatta
    10501 aggaatatgc tgattttttt atgtttaatc atacaaaata gttttttcat aaaaatatgg
    10561 aaaatatgga ttaattttga aataattgat ttaaaaggaa tacaaaagag tgaatagtag
    10621 gaaatagtgg tttagaacta gtgggctgat tttttatata tt
//
LOCUS       DAYYCW010000095        10328 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000095 DAYYCW010000000
VERSION     DAYYCW010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.097"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            173..322
                     /locus_tag="ACHAYA_002314"
     CDS             173..322
                     /locus_tag="ACHAYA_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM8606072.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            333..1847
                     /gene="dltA"
                     /locus_tag="ACHAYA_002315"
     CDS             333..1847
                     /gene="dltA"
                     /locus_tag="ACHAYA_002315"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM8606073.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1844..3049
                     /gene="dltB"
                     /locus_tag="ACHAYA_002316"
     CDS             1844..3049
                     /gene="dltB"
                     /locus_tag="ACHAYA_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM8606074.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3094..3327
                     /gene="dltC"
                     /locus_tag="ACHAYA_002317"
     CDS             3094..3327
                     /gene="dltC"
                     /locus_tag="ACHAYA_002317"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM8606075.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3330..4598
                     /gene="dltD"
                     /locus_tag="ACHAYA_002318"
     CDS             3330..4598
                     /gene="dltD"
                     /locus_tag="ACHAYA_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM8606076.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4739..5821
                     /locus_tag="ACHAYA_002319"
     CDS             4739..5821
                     /locus_tag="ACHAYA_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM8606077.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5821..6624
                     /locus_tag="ACHAYA_002320"
     CDS             5821..6624
                     /locus_tag="ACHAYA_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8606078.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6694..7434
                     /locus_tag="ACHAYA_002321"
     CDS             6694..7434
                     /locus_tag="ACHAYA_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8606079.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7445..8449
                     /locus_tag="ACHAYA_002322"
     CDS             7445..8449
                     /locus_tag="ACHAYA_002322"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM8606080.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8494..>10328)
                     /locus_tag="ACHAYA_002323"
     CDS             complement(8494..>10328)
                     /locus_tag="ACHAYA_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606081.1"
                     /translation="NNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSPADSN
                     QMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGN
                     HQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLP
                     PKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLS
                     CFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGI
                     FCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVA
                     CTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDR
                     RQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEK
                     ILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQL
                     LIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELD
                     DQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3322 a         1703 c         2087 g         3216 t
ORIGIN      
        1 ttttaaaagt acgagaaatt ataaagaaaa aagcagtgat ttgatgtgct atttttataa
       61 aagctatgtg acaaaactgt tagtaaaaaa acggtttctg taagctagac acatgtttct
      121 ttttgtcaaa attagttaca ctttgtgtga ggttatatga ggagggaatc caatgaacaa
      181 atggcgttct aatccaaaat tgaattattg gggaaaattc atcattcgaa caatttttta
      241 tagtgcagta ctgctgttcc tgatttatct gtatcactat aaaaatattc aaggaggaac
      301 ttttatctat aacgaatttt aggaggaaga gtatggaaat caaaacgatt attgaagcaa
      361 tcgatgattg gggaataaat gaacctgatc gagttgctta tcaggcggat gagacgcata
      421 cttttggtga attgaaacaa gcatcagacg cacttgctta ttatttagaa aaaaaggtag
      481 aaggcgatgg accaattgtc gtttttggca atttggagtt tgaaatgatt gtctcatttc
      541 taggggttgt caaatctggc catgcctatt tgccgattga agagcataca cccaaggagc
      601 gtatcctatc gatctttcgg gtggctaaac catctatggt catcagtatc ggggactgga
      661 tagaagatat tccttcggtt cctgtcataa caaaaaaaga atttcaagaa atcaagaaga
      721 taccagtagg attttatccc agaaatagcg taaaaggtac agaaaatttt tatattattt
      781 ttacttctgg tactactgga gaaccaaaag gcgtacaaat cagtcatgat aatctagtca
      841 gctttgttca atggacgatg gaagactttg ggatcaagcc tggtatgcat ttcttagccc
      901 aagcgccatt ttcttttgat ttatcggtat tttctattta tcctgcatta gtttctggcg
      961 gaatgctgaa gccgttaaat aaagcagttg ttcaagactt tcgtcaatta ttcgcgacat
     1021 tgccagcctt gaagttgaat gtgtgggttt ctactccatc atttatggat atttgcttga
     1081 tggaacctac attcaatgga gaaaatgtgc cggaattgga catgttttta ttttgcggag
     1141 aagaattgac gaaaaaaact gcagaaagtc tacttgatcg attcccaaat gcacggatat
     1201 ataatactta cggtccaaca gaagcaactg tagctatttc aagtattcag attgaccagc
     1261 atgtactaga tagttatgac cgactaccga ttggctacgt aaaaaaagac acgaaagttt
     1321 ctattgtcca agaaggtaaa cctgtttcaa aaggagaaac gggtgaaatc attattgcag
     1381 gaccaagcgt atcaaaaggt tacctgaaca atcccgaaaa gacagctgct gcgttctttt
     1441 gtcaaaatga cttagcaagt taccatacag gggatgcagg tagactagat gaatatgggc
     1501 ttttatttta tgaaggacgt atggatcagc aggtcaaatt acatggatac cggattgaac
     1561 tcggggatat cgaacattat ctgctccaag acaaccgaat caaacaagct gtcgtcgtac
     1621 caaaatacca aggaacaaaa gtacagcagc tggttgcttt tgtcgtatta gaaaacaaaa
     1681 cgaaacagcc ggattttcag ttgactaaat ctatcaaaga acaattacta gaagttgtaa
     1741 tggattatat gattcctcag aagttccatt atgtcgatct gctgcctcag acagttaatg
     1801 gaaaaatcga tcgaaaaaaa ttaacagctg aggtgaatcc tgaatgatgt tacctcatat
     1861 gatcccttat gcagagccgt tttatttttt tcttttagcc attgcgcttg taccgatcgt
     1921 tctatcttta ttgatatggg aaaaaagatt accagtttat caatcagcag tgacactttt
     1981 ctttttattt gtcagctttg gaggtaatta ttggcaacag ggagcggcgc tgattgggta
     2041 tgttttgtgg cagacgatac ttgtatgggg ctattttgtt tatcggcaaa ggaaaaatca
     2101 gacaggatgg ttttttgccg ccgttatttt agcgataatt ccactgtttt taacgaaagt
     2161 gactcctttt tttgaacatg gaaaagcatc gttacttggt tttttaggaa tctcttattt
     2221 aacctttaaa tctgtacaaa tgatcatgga aataagagat ggcatgatca aaacatataa
     2281 gccatttcgt tatatccagt ttctgctgtt ctttccaaca atctcttctg gaccaatcga
     2341 tcgctatagg agatttgaaa aagatcagct ccatccgcct gttcgtgata aatatgttga
     2401 tcttttggga aaaggcgtcc aaaatatttt tgtcggtttt ttatataaat tcattatcgg
     2461 ttattatttt ggtcaagtac tgcttcctgt agtgggacgc attgccttac agcatggtgg
     2521 tatttcatgg gcattagtag gttatatgta cgtgtacagt atgtatttgt tttttgattt
     2581 tgctggctat agcttatttg cagtcggtac aagctacatg atgggatatc agacaccaat
     2641 caatttcaat aaaccttttt tatcatggaa tatcaaagaa ttctggaatc gttggcatat
     2701 gacgctgtcc ttttggttcc gtgattatgt atatatgcga ttgatgttct tcatgatgaa
     2761 gaaaaaattt tttaaaagca agatcgtaat gtcaaatatc ggttattttg ctttgtttct
     2821 tctaatgggt atatggcatg gattgacgtg gtactacatc gtatatggac tgtatcatgc
     2881 ttgtctgatc tgtctgacag atgcatggat ccgatttaag aagaagcata aagagcagtt
     2941 gccttctaac aaatttacgc atggcttagc cgtatttttg acatttcaag cagtatgttt
     3001 tagcttcctg attttttcag gatttttaga taaacttttt tttagctaac gaatagtgac
     3061 atattaattt atataacaaa ggagaaaagg aaaatggaag aacaaatttt atcaatctta
     3121 gaagaaataa caggaacaga tgaagtaaga aaaaatcgtg atattgatct atttgatgaa
     3181 gggttgcttg attctttagc atctgttcaa ttattagtcg agctagacgg agagctggat
     3241 atccaagtcc ctgtatctga attcgagcga gaggattggt gcacaccgaa taaaatcatt
     3301 caacaagcaa ctgcattgaa gggttgagta tgaaaaagaa actttttgct attttcggcc
     3361 cggttgtttt agctgttggc ttactgtttg tgttttttac ctcctcctat caagtagata
     3421 tctctaatcc agaaacgatt agacgtgcct cttcttccat gtcagtgaac gtgttgaaag
     3481 gagatgcgat aaaaaattat gcattatctg aaaaacaata tattcctttt tttggctcaa
     3541 gtgaattaag cagaatcagt ccatttcatc cgtctgttct ggcagaaaaa taccagcgaa
     3601 actatcgtcc atttttgctg ggagcacccg ggacacaatc gttaagccaa tatatgatga
     3661 tgcgttctgc aggagatgct atgaaaaaca agaaagtcgt ctttattatt tctcctcagt
     3721 ggttcgttaa aaatggtgta aaaactgatt actttaatac gtattattct gaacttcaaa
     3781 catacgactg gttgttttca atgaagaaag taacgcctgc tgatcgctat ttagctagaa
     3841 gattattaac tttctcaaaa gtaaaagaga atgatacgtt gacggcgatt ctgcaaacga
     3901 tcaaaaaagg gaagctacct ttacctgaat ctttgaatca gctgcgcagc caatggaaca
     3961 tgctgaagag agaggatgaa gtattcagca atattgggtt gaaagatcga cagcaaaaaa
     4021 tcgatcatga gtcaaaacga ttaccaaaac aataccaaga aacagaatta tccattttag
     4081 caaatcaaat tggagaacga gaaacgacga ataatccatt cgggttgaaa aacgattttt
     4141 atacgcatcg tatccgagcc cacgagccag agctcaaaca gtcacaaaaa aattgggatt
     4201 atcgtttttc gccagaattt tctgattttc agctggtctt ggatcaatta gcaaaaaacc
     4261 ataacgaagt attgttcatc atcccaccgg ttaacgagaa atggagcgat tataccggac
     4321 tatctcaaga aatgttacaa ggatttgcca aaaagatcaa atttcaatta aatagccaag
     4381 gattcaatcg aatagctgat tttgtcaatc aagcgggtac caactatttt atggaagaca
     4441 cgatccatct aggctggaaa ggctggttgg ctgcagatca acagatcaga ccgtttttag
     4501 aagaaaatca tattactgct agtaagtatc atcttgacga tgcttttttt agcaaatctt
     4561 ggcaacatca aataccagat aaactacaat taaaataaag aaaacaggcg agtgttgctc
     4621 gcttgttttc tttatttttg aatattctga ataaaagata aaaataccaa gtttgcctga
     4681 acaaaaatca taagcaatgg tataatcaca atatgaatat agaaatgggg gaaaacattt
     4741 ggatacaaaa gaagaacagc tacttattga tttaacttct gcaaaaggag tacctggaaa
     4801 tgaagaagag gtaagagaag tattcagaga atatgccaaa ccattcgctg atgatatttt
     4861 ttatgacggt ttaggcagtg tgattgccaa acatggtgct ggcggaggcc ccaaggtctt
     4921 tatttctgga catatggacg aagttggttt tatggtaaca aaaataaccg aaaaaggatt
     4981 cttggaattc caaactcttg gaggttggtg ggggcaagtc atgcttgctc agcaagttga
     5041 aatcaaaaca cgtgaaggga aaatcgtcca cggtgtgatt ggttccaaac caccacatgt
     5101 tttaacacct caagtaagaa ataaacctta tgaaataaag gatatgttca ttgatattgg
     5161 cgcatcaagc caagaagaag ccaaagagtg gggcatacgt cctggagata tggtcactcc
     5221 atatattgaa tacaaacgaa tgaatggcag taaatattta ctggcaaaag catgggataa
     5281 tcgtatcggg acagctgttt ctctgcgagt attagagaat ctatctaaag aagcacaccc
     5341 aaatgtttta ttcgcaggaa gcgatgtgca agaagaagta ggcttgcgag gtgctcgaac
     5401 aagtacacac cttgtaaatc ccgatattgc ttttgcttta gatactggta cagcaggaga
     5461 cactccagga atgacaccaa aagaagcaga ttcgatttta ggaaaaggcc cacagatttt
     5521 gatttttgat gcatctatga tcccgcataa aaaattattg aattttgtta ttgatgtagc
     5581 agaggaaatg gatattccat tccaatatac agtgatttct ggcggaggaa ctgatgcagg
     5641 acaaatgcat ttgacaagag atggtgttcc gtctgtagcc attaccgttc ctgttcgtta
     5701 tctgcattcc catacatcag tgattcatga agatgattat ttgaatacag tgaaactagt
     5761 cacagaatta gtgaaacggc ttgatagtga aacagttgaa aaaattcgga gttatgaata
     5821 atgaataata gaatacctat ttctgaatat cggaaaaaag cacgagtttc tttggaaaat
     5881 caatggggaa tcaatacttg gcttgttttc ttatctataa tatttacgag cgtcatccag
     5941 aacttttttg acggtttcag tccttttccg gaagaaagca tgcagcagca cttattgagt
     6001 tttctagtcg ataatttact tttattcgca ttcaactatg cattgtttta cattgcgcta
     6061 gtagttgtac ggggtggcag accaaaaagt aaccttttat tttcagtttt ccaaagaaag
     6121 tattttggtt cgatacttgg catcaatttg atcaattcag tcatcaactg gctactcaac
     6181 agtttaatct ttctgcccat gtttttaatc agcggagtga gtacttatat gcagctgctt
     6241 ttcagttcaa atgcggtacc tacgggtgtc atgccagaag attttcttga tatcagcttt
     6301 gttttatcga tgatagggat gatttttgtt tcagcactca tcatgagtat tgttggcggt
     6361 ttattccaat ttgctgcatg gacaaagttc gactatcctg atttgtcggt tattcaatgt
     6421 ttgaaatatg gctggttttt attgaaagat agaattggta cgtatatttt gttgcagctg
     6481 tcttttatcg gctggtattt gctaggagca atcgctttgt tcatcggtct tttgtgggtc
     6541 attgtttatg tgaatgtgtc agttgcagaa ttttatgaac aagcacggat tgaaaaagga
     6601 ggtccagacg cctattttac ttagcatgta agcagtttat tcatcaaaaa aataactttt
     6661 gtggtaggat aaatctttga gggggaatgt acgatgaaat taactgtttt aggatgctta
     6721 ggagcgtatc cttataaaaa tcaagggaca acaggttatt tgcttcaatc tgacgggttc
     6781 aatttgttga tcgatgcagg tagtgctacg ctgatcaaat tacaagaaca ccttgatcca
     6841 cttgatctag atgctgtgat ccttagccat tatcatcatg atcatattgc tgaccttggt
     6901 gtattacagt attactggca acttcatcct aagagaaatc cggagacatt gctttcaatt
     6961 tatggacaca caaaagattc cattcacttt gaagaattga cgttggaaaa tgtctcaaaa
     7021 ggacatcctt attttgaagc tgaagaatta aaaattggtc cttttctgat tacttttatg
     7081 gaaacaatcc atccagtagt atgttacgcc atgcgtatcg tagaagaaac aacggggaaa
     7141 gtattcgtat ttacaggtga ttcaggttat ttggaaagtt tcgctgattt tgcgaaagat
     7201 gctgatttgt ttctagcaga tacctatctt tttgaaggaa atgaacatca tcatgcccat
     7261 ttcacttcaa aagaagctgg agaacttgcg aaatcagcta acgtaaagaa acttgtactt
     7321 acacacctac ctcagtttgg tgatttagaa caattgaaaa atgaagcgca aaaggcagca
     7381 ggcaaaggga ttgatataga acttgctgca gttgacaaag tgtttaacat ttaggaggaa
     7441 gaacatgatt tttgtaccaa atgaaaacca cgacccaaga atcaatttag caattgaaca
     7501 atatttgctg caagaaatgc ctttagatga acctattttg ctcttttata tcaatgaacc
     7561 ttcaatcatc atcggcagaa accaaaatac gcttgaagaa atcaatcgag attatgtgga
     7621 agaaaatggc attcatgttg tccgtcgctt aagtggaggc ggagctgtct accatgattt
     7681 tggaaacctg aactttagtt ttatcatgcc tgatgatggg gattcattcc gtgactttgc
     7741 gaaagtaaca cagccaatca tccaagcact tcatgaattg ggtgttgctg gggcggaatt
     7801 gaaaggccga aatgatctag tcattgatgg catgaaattt tctggcaacg cgatgtatgc
     7861 taccaacggt cggatgtttg ctcatggaac aattatgttc gactctgata tcaatgaagt
     7921 agtcaatgca ttaaaagtaa aaaaagacaa aatcgaatca aaaggaatca aatcgatccg
     7981 ttctcgtgta acgaatatca aaccatttct tccagaagaa aagcaaaaaa tgacaacaga
     8041 agaatttcgt caagaaatct tgttgaaaat tttcggcgta gagtcagtag aagaagtaaa
     8101 aacgtacgaa ttgactgatg aagattggaa aaaaatcaat cgaatctctg aggaatatta
     8161 tcgaaattgg gattggaatt acgggaagtc tccagatttc aatttctctc gccaaaaacg
     8221 tttttctatc ggttcgattg aagtccattt gaatgtctca gaaggaatga tcaaagaagc
     8281 aaaaatcttt ggcgatttct tcggactagg agatatctct gacgtggaaa aaaaattggt
     8341 tggacagaaa tatgataaag catctctatc aaaaatagtc agccaaattg atgtgaagaa
     8401 atattttggt gcgatagaac cagaagaatt gcttacctta ctttattgaa aataaaaaat
     8461 agcggaatcc acatatatgg attccgctat tttttataca ggattcgggt aatttcgaga
     8521 gagttgaaca gtgatccgat caatgaaagt atctttttcc tgcgttagtg ttcctgtgta
     8581 acgatccgct acgtctttca tcatcattaa gtcaaaacgt tcctgatcat ctaattcttc
     8641 tgtgatgcct cgactaaata ggtttgtatc gtaatgtgca ctatgttcgc aacaaatgac
     8701 gaaatgttcc tcttgttcgt gtatcgacaa ataaattttt cgatacagtg gattttcgat
     8761 ggcatgagtt tctctaaagg aatgctctaa agcgtggatc aaaagttgag ccagatcgtc
     8821 atcgttgata ggaagttctt cgggaagatc tgcttggaag gttatctgaa tattccgttt
     8881 ggcagctaac ttctggtaac gcgctaagat aagatttgtc acctggtggt tgcaaaaaga
     8941 acacagcatt tcgaaatttt ttgcttcttc taatatcttt tccacatagt ttttagcttt
     9001 tgtttggtcc gtatgcatta aatcttgcag attctccatc tcttggacaa attccaggcg
     9061 aagtgtattt acttcttgga tatgctgtcg aagctgctca taatgttgct ccaacaaatc
     9121 atttttgatt tttaagaaat ctacttgtct ccgatctttg tcggtattta agatgtgatc
     9181 gattagattg tagctgataa taacgattag aatgataata tataaaatcg tagaccaatt
     9241 gatcgttgtt tgatagatac ctaatgccgt ttggatgtaa atcaagcaat ggataatggc
     9301 taccaagacg atccaaggtg tacaagctac ataaaaacgg ttattcttat atgcttcgcc
     9361 aatgcatgca agtgaagtga ctaaagtgct tataacacta gcaatattag caatcggcat
     9421 taattcagga agttctgcag gagtaaaagt tgcataaagc agaacgccga taaaaatcaa
     9481 aagttggaac gtacaaaaaa ttccgtacca ttttttatag taaaacattc ggctccaata
     9541 gtagcagatg atgaaagctg acgttaagat aataaataaa tgagataaaa aagaatgaac
     9601 gaatggttca aaaagaatac cacttaaaat atcttctgat acagcttcaa atcctaatgc
     9661 caaagcaaag caacttaaga gaataagtga gtattcaaga cgtttttgtt tatacatcaa
     9721 gtagatcgta tagaaaaagg tccctatagc gataaaaaca gcgattatca tcgtcaatac
     9781 ttgcggcact gattgtgaat aaataaaact aatcaatggt ccagttgttc ctagataaac
     9841 tttcggtggc agtccagcat agttctgata aggagatgag actgtaaggg tcaatttttt
     9901 tccgatataa tttgctggta aatctatcac agccaatgag agtccgggat tgctttcttc
     9961 ctgataatct ttccgttggt ataaaatctt ttcgcctaga tatacagtaa tccattgatg
    10021 gtttcctttg attagtaaat ttgcttcgtc caacggtttt tcaagtgtcg tttccataat
    10081 gaatgtttcg tctttatcca cggtaggaat cgtacgagtg tacttgctgt caaacttaaa
    10141 atcaggatca tcttttgtat agaaaaccca cgaatctttt atttctttca tttgattgct
    10201 gtccgcagga gaaaaaataa aataaaagaa ataagcaatt cctgttaaaa ataacaatga
    10261 taatataatg attatgctcc actttatacg taaataaaaa gattcttttt tattatatat
    10321 gttattaa
//
LOCUS       DAYYCW010000096        10024 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000096 DAYYCW010000000
VERSION     DAYYCW010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.098"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..808
                     /locus_tag="ACHAYA_002324"
     CDS             2..808
                     /locus_tag="ACHAYA_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM8606082.1"
                     /translation="MKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPNVKKGFFEAE
                     VDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALANLHQQTAPQF
                     GFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQEKYLRFKERV
                     LKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAMSQLFGGFRP
                     EFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLENF"
     gene            824..1561
                     /locus_tag="ACHAYA_002325"
     CDS             824..1561
                     /locus_tag="ACHAYA_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM8606083.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(1716..2684)
                     /locus_tag="ACHAYA_002326"
     CDS             complement(1716..2684)
                     /locus_tag="ACHAYA_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM8606084.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            2916..3749
                     /locus_tag="ACHAYA_002327"
     CDS             2916..3749
                     /locus_tag="ACHAYA_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM8606085.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            3783..4622
                     /locus_tag="ACHAYA_002328"
     CDS             3783..4622
                     /locus_tag="ACHAYA_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8606086.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            4648..5208
                     /locus_tag="ACHAYA_002329"
     CDS             4648..5208
                     /locus_tag="ACHAYA_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8606087.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            5407..7128
                     /locus_tag="ACHAYA_002330"
     CDS             5407..7128
                     /locus_tag="ACHAYA_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM8606088.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            7293..8435
                     /locus_tag="ACHAYA_002331"
     CDS             7293..8435
                     /locus_tag="ACHAYA_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8606089.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            8451..9662
                     /gene="thiI"
                     /locus_tag="ACHAYA_002332"
     CDS             8451..9662
                     /gene="thiI"
                     /locus_tag="ACHAYA_002332"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM8606090.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         3426 a         1696 c         2104 g         2798 t
ORIGIN      
        1 tctgaaatta aatggtaaag tgattccagt tgttggtggc gatgttaatc agacctatcg
       61 tataaaaacc gaacatcgtg cttatttttt aaaaatccat ccgaatgtaa aaaaaggatt
      121 ttttgaagca gaagtagacg gattaaaaga actgtcagca ttcgtacgtg taccggatac
      181 gtatatgcta ggtgaaacaa gtgaaggcgc ttatttgtta atggaatgga tcgagccggg
      241 aaaaggggat caacgagatc ttgctgcagc tttagctaat ctccatcagc aaacagcgcc
      301 acaatttggt tttcgtaaag ataactattt gggtacactt gttcagaaaa attcttttga
      361 agaagattgg tggacttttt tctttaaaga tcggcttgaa tcccagattt ctttggcaga
      421 agaaacgaat cgatggaatg tacagcgtca agaaaagtat ctgcgattta aagaacgtgt
      481 attgaaatcg gttgaaccaa aaaagatcac tccacgcctc ttgcacggag acctgtggag
      541 tggtaatgtg ttctttgacc agcaagggca tccagttttt gtcgacccag ctgtttctta
      601 tgggaatcgg gaacaagata ttgcaatgag ccagttattc ggtggtttcc gaccggaatt
      661 tttagatgct tatcaaacta tatttccatt agaaaaggga tggaaagacc gactgccgat
      721 ttaccagtta tattatcttt tggcacattt gaatatgttt ggagaaagtt acggttccca
      781 agtagatcaa ttactagaaa atttttaaga ggagggcgtg tttttggcaa aaaaatatac
      841 caaaaagcat gaagtttcat attatgagtg tgacatcaac caaacgatga catttccttc
      901 gatgttagga gtagttatca aaacgtctga agatcaaagc gaccagttgg gaagaggcag
      961 tgaattcgtc aatagctttg gacttacttg ggtcatcacg aattattcca tggagattaa
     1021 tcgtctccct agagtcggtg aagtaatctc agtaacgact caagccatgg agtacaataa
     1081 gtttttctgt tatcggaact tctggatcca tgatgaagat gggaaagaat tagtcaaaat
     1141 agaatctgtt ttcgtattga tggattttgt gaatcgaaaa atgagcagtg ttaatgaaga
     1201 gatcattgct ccttttgaga gtgagaagat caagaaaatc aagcgtcagg aaaaaattga
     1261 aaagattgat tcgggcttca tgttgccata tcgtgttcgt ttttatgata tcgatagtaa
     1321 tcagcatgtt aataatgcga tgtattttaa ttggatcatt gacgtattag gatatgactt
     1381 tttgactacg catgttccag aaaaagtaat tattcgattt gataaagaag tagagtacgg
     1441 gaatgagatc gaaagccatt ttgaacaaat ccctatcgaa aatggtgtca aaacaaaaca
     1501 tgaaatccga atgcaagaac agctgtattg tgaagcaaat attatatgga aaaataaata
     1561 aaaaagaggt tgtgacagaa gggggcaact tcaagaaata agaagaaatt tacgaaaatt
     1621 gtttctcaaa tttttgtaaa ttttcgactt agttttcgaa gaagctattt ttgtcacagc
     1681 ctctcttttt aagtttagct attagataat gatgtttagt tttggataaa cgaaatcaat
     1741 tcttcacccg acaaaccata gtttacagca atcaatagtt ttaaacgtgc tttttgacta
     1801 ttaatactgt tacagaaaat aatccccatt ttttctagtt cttttccccc gccttcataa
     1861 tcataaacag gctctgcgat tccattgaaa caacgagaaa caagtactac agggattttt
     1921 tggacaagaa gcttttccaa agcagctaag gcttgtggag ggagattccc cgcacctaaa
     1981 gcttcgatta ccaaaccgtc tactttcgta tgggcgatgg cttccaataa gtccccttgc
     2041 atcccagcat atgctttaac gatcgggata gtcccatcta cagcggaaat gtccagtcgc
     2101 tttgtttcta aaagttcctg caagaataaa attcgattct tagttactaa gccgatggga
     2161 cccaatgtag gtgttcggaa agtagcaaca tttgtggtat gggtctttgt cacgtagcgt
     2221 gcagaatgga tctcatcgtt catgaccacc aaaaccccct tatctagcgc ttcttcacaa
     2281 cttgcgacac gaatcgcact ttcaaaatta taaaggccgt cacttccaag ttcattactt
     2341 gaacgcattg ccccagtcaa aacaattggt aatttgttgc caatcgttgt atcaagaaaa
     2401 aaagcagttt cttctaatgt atctgtaccg tgtgtgatca ccactccgga tatacctgat
     2461 gcataagcgg acaagatacg ttcttttaat accaacatgt gagaaggcat gatgtgtggt
     2521 gagggaagat tgaagatatc ttcaactaat aattctacat gcttggggat tgtgatttct
     2581 gcatctaata atgggtttgc cacatcagga ctaacaccgc ccgtgagcat gttttctttc
     2641 atggcgatcg ttccgccggt gtgtagtaca agtatttttt tcattttaca gacttcctta
     2701 cgaataattt tgcatagccg ttaaatctta ttgtatacta tataaaagag ttttgctatc
     2761 agtgaagaat aacgaatagc ctaacttatc ataaaaaagc tgaccttatt aaaatggaaa
     2821 agactcaacc ttgacctcct aatacaatgg agtatctggt tgagtttctt ttacaagaaa
     2881 gcagccaagg aaatcaatca cgaaggtggc cggaaatgat cagaatcgga ttaacatcgt
     2941 ttaaagaaca tgacaaatta actggtaaaa agaataacac tctttatgaa tatgcagggt
     3001 ttctaccctt agtggaatta gatactgcct attatggaat tccccgaaag tcctcagttg
     3061 agaattggat aagtgaagta cctgaatcat ttcgctttgt agtcaaagtc tatcaaggaa
     3121 tcagcggtca aggagactgg aagccttatt atcagaccga agaagagatg attgcgcatt
     3181 ttttagatgt tatgtcgcca atggtagatg ctggaaagat gttttgcttt ttagtccaat
     3241 tcccctcatc attcaaatgt acgaaagaaa atgtccagta cttgcgcaaa cttagaaagt
     3301 ggtttgaggg ttatcctgtt tctatagaat tgcgggatta ttcttggtac agcgatgaat
     3361 ttcttgaacg tacaagaaac tttatgtcta gagccgcttt ttctttagcc atcatcgatg
     3421 agccacagtt gctgaataca acgattcctt ttgatccatt tgtcaccaat ccgaactttg
     3481 ctctttttcg attccatggt aggaatgcgc aaggatggca agcaaatgat aaagagtggc
     3541 gtaaaaaaag aacgttatat cgttattcag aaaaagaact gcaagaacta gcacatgaag
     3601 tcaaagtagt tgattctaag acaaaagagg taggtgtgat ttttaataat aattctggtg
     3661 gtgatgcggc tgaaaatgca ttagcactgc aaaaaatatt aaatatcaag ccagatggac
     3721 tcaatccaac ccaaatggat ctgttttaag ggcttatttt attcaagaaa ggggcgaagt
     3781 ctatgatcaa agctattttt tttgatattg acggaacatt ggtcaccaat cgttcaaaag
     3841 cgttggaatc aacaaaacaa gcaattgaat atgctcggaa aaacggcatt ttatgcggtg
     3901 tagcaacggg gcgcagccca gtaaagatca aagaaatcat tgatgaattg gaattagata
     3961 tgtatgtcgt gtataatggt caactggttt ttactgcaga tcgcacgatt atcgaccacc
     4021 cttttgaaca aaaagtattg gaacatattg ttgaatttgc agatgagaac catcgacaaa
     4081 tcgtttttgg tgcgagaaat cggttagatg gatcaacgac gatgctatta ggacaatcga
     4141 tttttatcaa gcgactagtc agctttttac cgcgtaaatt cccggtacgt ctgatgaaaa
     4201 aaatcctcca ggtttttagc cctcaccgac aaaaggatcg ttatgaaacg ttagagatat
     4261 taaaagaacc aatttatcaa tgtatattgc tcagtccgga gtcagaacag aaaaagttag
     4321 aacatcgctt tcccgagtgt acgtttcagc gttcaaatac gtatactgta gatatcatcc
     4381 caaaaggtgg ttcaaaatta ctaggcatcc aagctttcgc aaatgctgtc gaaattgaaa
     4441 tggaagaaat tatggcattc ggcgatcatt acaatgatat cgagatgctg aaaggtgtag
     4501 gaattggagt ggccatgggg aatgctcaga tcgaagtaaa acatgctgca gactttgtga
     4561 ctcaatcaaa tgaagaagat gggatatacg aaggactgaa acacttcggt ctgatttatt
     4621 agtcaatgaa agaaggaaaa aatcagtatg gaagcagaga caccttttca aaaagtagaa
     4681 acattccaca aaacttttga tccaagaaga ccagagatac ccacggcttt tacagaaaat
     4741 gaagctcata cacgtgctgc gttcaaaata gaagaacttg ttgaatttgt tgcagctact
     4801 tcacctaata aagaacaact gcatgaagct gttatgctgc tgcacgaagc attagataaa
     4861 gcagaagaaa aaatacttgg taaaacacag tggggaagtg atccgctggt tgaacaagta
     4921 gatgcactga cagatatcct ttactttact tatggttctt tttcattact aggaatagtg
     4981 cctgatcgaa tattcgaaat cgtccatcag gcaaatatgg ggaaattatt tccagatgga
     5041 aaacctcatt atgaccctgt aacaaataaa gtgttgaaac ctgaaaactg gcagatggaa
     5101 tatgctccag aacctaaaat cgcggcagaa atccaacgtc agattcaaga agcgattgct
     5161 aagaagaaat ccgctgatat aggcatgtgc acaaatgaaa aagagtgatc agagtttcac
     5221 tatttttgtt atctttatac gatggaaaaa caaaagattt tcgtttaagt tagtgaatgc
     5281 atgaaaatag tatgctataa tctagaatag atgtcttttg cacgagagac gtattatttg
     5341 gatttatgcc aagcagaacg ccatctgttg cataaaagat gcatactgaa aacggaggaa
     5401 ccataaatgg gaaataatgt tatcatcgga atcgtcattg caatcattat agtcgccatt
     5461 gtcgtctttt tggttagtta cttgatgaag aaaaaaaacc aagatcgatt ggatgcctta
     5521 gaaaaacgca aggaagaatt atttgacttg ccagtgattg aagaagtcga tgatgttaaa
     5581 aaaatgcatc tggttgggca aagccaaaat acctttcgtg aatggagtca aaaatggaat
     5641 gacatatcta cttcctcgtt cgctgaacta gaaagtcaaa ttttcgaagt ggaaaacctc
     5701 aatgaaagtt ttcggttctt aaaaggaaaa aaagcagttg ttgaagcaga agctacaatg
     5761 gaccaaatgg agaaacaagt gacggaaatc cgtgaaggtc taaaggaatt gcgggaaacg
     5821 gaagaacgca actcactagc tgttcagcag gcacttgacg tctatgaaga actgaaagaa
     5881 aaagtcagag aagacgagca tgaatacgga ccagctcttt ttgaattgca aaaacagatc
     5941 aaagtgatcg aaaaagaatt tacgcagttt gtgactttaa acacttctgg agaccctgtt
     6001 gaagcccgcg aggttttgga taaagcagaa aaacatacgt atgaagtgga agctctgatg
     6061 aaaaaaattc caccgctgta tgaggagctc cataccactt tccctgaaca acttgaagaa
     6121 atcagtgata cttacgagaa attgatggaa gaacaatatg tattcccaga agaagatctt
     6181 gctgaagata tcgcaaaagt aagtcgaaga attgaaaatt cattagccaa tcttgaaaaa
     6241 acagaagttg aaacggtcga atttgaaaac cgtgaaacag ctgatttgat tgattcgtta
     6301 tatgatatct tagaacggga aatggaagct cagagatatg taaaaactaa ccaatcaacg
     6361 attgcagagt atatcaaaca tacaacaaag aataaccgtc aattattgat tgagctggat
     6421 cacacatctc aaagctatac attgaaccac aatgaaattg gccgtgtacg aggtttccaa
     6481 accgaagtgg aagaaatgga acgtcaaaat gaacagatga ttccacagat ccgtcaacac
     6541 gaaattcctt attcagaagt gcgcacgttc tataaaacag tcttcaaagt cttagaagat
     6601 atcgaaaagc aacaagtaga gatcgacgac tcattacatg aattgagaaa aggcgaaaaa
     6661 gaagcgcaag aaaagatcga tacattcgaa ttcaaattgc gcagtttgaa acgttttgta
     6721 gaaaaacaac gtttgcctgg cttgccgaat gattacctag aattcttttt tgttgctact
     6781 gaccgtattg aagaactaag tgttgtcttg aataaaattc gagtaaacat ggaagaggtc
     6841 aatcgtttag ttgctctatg tgaagaagat ctagaattat tagataagaa gacacatgat
     6901 ttagtagatg cagcagcatt aacggagcaa atgctccaat atgcaaatcg ttatcgtcat
     6961 acacacgaag aagtgagaga agcaattgat aaaagctact atttattcaa taaagaatat
     7021 cactatcaag aagcattaga cgaaatcggt acagcattag agcgagtaga acctggtgct
     7081 ttcaaacgca tcgaagattt ttattttaat catccagact tagtataaag caaaaaagtt
     7141 agtaaaccag caagagcaag acggattatt gtcttgttct tgtctttttt tgtatatttg
     7201 aaaattgaaa caagtgaaaa tttcacctgc tcttgaaagc accttggaca accgttataa
     7261 tagaaaagaa tttcaaaaaa gaggggtttc ttatgatata ttttgataac agtgcaacaa
     7321 ccccaatctt tccgcaaagc ttagatgcat atgtaaaaac cagtcagcgg attattggga
     7381 atccatcaag cttgcatgat ttaggcaatc aagctaaccg gttgttgcag caagcaagaa
     7441 agcagatcgc tgatttgatc catgtagaac cagaagaaat ctattttact tcaggcggaa
     7501 cagaaggaga taactgggta ttaaaaggaa cgatgatcga aaaaagagaa tatgggaatc
     7561 atatgatcat ttcaggagtc gaacatccgg ccgtcagcga gacagcagaa caattaaaag
     7621 aattaggaat cgaagtaagt atcgccccag tggataaacg cggatttgta cgtgtcgacg
     7681 aattgaaaga attaataaga aaagagacag tattagtctc tgttatggct gtgaataatg
     7741 aagtaggatc gattcagccc atccaggaaa tcagtgatct tttggatgat tttcctaaaa
     7801 ttcatttcca tgtagatgct gttcaggcga ttggaaaagt accattctcg caatggttga
     7861 cagaaagagt tgattttgca acattttcag ctcataaatt ccatggccca cggggaactg
     7921 gtttcattta ttggaaaaag ggtcgtcgtt tagcaccatt actgacaggt ggagggcaag
     7981 aaagaaatca gcgaagtggg acagaaaatg ttccaggtat tgtggctatg gttaaagctt
     8041 tacgtttatc attagagata aaggaacaaa aacctgagca gacacaacgc ttaaaagtag
     8101 ctctaatcaa tgcgttgaag acgtatgaga aagtgacgat tttttcagaa ggaactgact
     8161 atgcaccgca tattttatgt tttgctctaa aaggaattcg cggcgaagtt ctcgtccatg
     8221 ccttagaaga aaaacaaata tttgtttcta ctactagtgc ctgctcctca cgtaaacata
     8281 tggcaagcag tacattgcac gcaatgcatg taccaaatga tctggctaca tctgctgttc
     8341 gaatcagctt agatgaaaac aattcgcttg cagaagtcga gcaatttatg attattttca
     8401 agcagcttta taagaaattt tctgcaatca attaaaagag gagtgttctt gtgaaataca
     8461 gtgaaattat ggtccgttat ggggaacttt cgacaaaagg aaagaatcga aaaagcttta
     8521 tcatgcaact agcacaaaat gtcagacaag ctttgaaaga ctttccagaa atcaaaatcc
     8581 atgctgatcg ggaccggatg catcttttat tgaatggcgc agacgggcag ctagttattc
     8641 caaaactagc aaaaatattt gggatccaaa atttctctcc aagtattcgt gtagaaaaag
     8701 atgtcgaagt tttgaaacaa gctgttcaag acatcatgaa agaaatttat actgggaaag
     8761 aaacatttaa aattatcgct aaaagaagcg atcaccaatt tgaattgaca agtaatgaat
     8821 tgaatcaaac attaggtaat gctgtttttg atattttccc gcatatccaa gttcaaatgc
     8881 gtcaaccaga tattccatta cgtgtagaga ttcggcgtga tggcgcttat ctatcgtatg
     8941 aaacgatcaa gggagctgga ggattgcccg taggaacaag cggaaaagga atgcttatgc
     9001 tttctggggg gattgattct cctgtagcag ggtactttgc catgaagcgg ggagtggaaa
     9061 ttgaagctgt tcattttgct agtccgccgt atacaagtga acaagcactg caaaaagcaa
     9121 aagatctaac agccaaacta gcaccttatg tgggaacgat ccattttatc gaagtgccat
     9181 ttaccgagat tcaagaagaa atcaaaaagt cagtgccaca aggatattgg atgacaatta
     9241 ctcgtcgaat gatgttgcgt ttgaccgatg aaattcgccg tattcgtcat ggtttagtca
     9301 ttttgaatgg tgaatcatta ggtcaagtgg cttcacaaac tttgcaaagt atggtggcta
     9361 tcaatgaagt aacgaatacg ccaatcattc ggcctgtagc gacaatggat aaacttgaaa
     9421 tcattgaagt agcagaacag atcgatacat ttaatctttc tatccagcct tttgaagatt
     9481 gctgtacgat ttttgctcct ccacagccaa aaacacgccc tcaactggaa aaggtccatc
     9541 aatatgaaga gcgtttagca atagatgaaa tgatccaacg agcactagct ggcttgaaga
     9601 tggaagagat acaagcagaa tcgtctaatg aaaaaactga agaattttca gaattgctat
     9661 aagaggttat gaaaatagtg ttttgaccag agtattagag aaacaaaacg aaaaaatagc
     9721 tccatgtatt ttaccgtttt tgaactaatg tcgaagatta gctgattgaa taccatttat
     9781 caagaagtta tggaaaagcc ggttcttttt tactaactga aaaataaaat tttatttaga
     9841 gatcaagcta cagttgataa atgatataaa aaagagaatt tcccatgaaa tagcgaggtt
     9901 gtgacagaag tgggcagctt caagaaataa gaaggaattt acgaaaattg cttttcaaat
     9961 ttttgtgaaa tttcggctta tttttcgaag aagttacttc tggaacaccg tgtatcagag
    10021 gttg
//
LOCUS       DAYYCW010000097         9932 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000097 DAYYCW010000000
VERSION     DAYYCW010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.099"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..388
                     /locus_tag="ACHAYA_002333"
     CDS             32..388
                     /locus_tag="ACHAYA_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HFM8606091.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            404..754
                     /locus_tag="ACHAYA_002334"
     CDS             404..754
                     /locus_tag="ACHAYA_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HFM8606092.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            783..1562
                     /locus_tag="ACHAYA_002335"
     CDS             783..1562
                     /locus_tag="ACHAYA_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HFM8606093.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1573..2235
                     /locus_tag="ACHAYA_002336"
     CDS             1573..2235
                     /locus_tag="ACHAYA_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HFM8606094.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAIFPA
                     VN"
     gene            2254..3360
                     /locus_tag="ACHAYA_002337"
     CDS             2254..3360
                     /locus_tag="ACHAYA_002337"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HFM8606095.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3350..4440
                     /locus_tag="ACHAYA_002338"
                     /pseudo
     CDS             3350..4440
                     /locus_tag="ACHAYA_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
     gene            4453..5193
                     /locus_tag="ACHAYA_002339"
     CDS             4453..5193
                     /locus_tag="ACHAYA_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HFM8606096.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5481..7421
                     /locus_tag="ACHAYA_002340"
     CDS             5481..7421
                     /locus_tag="ACHAYA_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8606097.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7508..9430
                     /locus_tag="ACHAYA_002341"
     CDS             7508..9430
                     /locus_tag="ACHAYA_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8606098.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(9710..>9932)
                     /locus_tag="ACHAYA_002342"
     CDS             complement(9710..>9932)
                     /locus_tag="ACHAYA_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFM8606099.1"
                     /translation="RDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAVGS
                     EEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         3242 a         1647 c         2206 g         2837 t
ORIGIN      
        1 acaaatgata ttttacgagg aggaaacaat catgattaaa gtagttattg cagaccgtat
       61 gggtaaagga caaaatgtgg ccaaaggggt agaagcagct ggtggaaaag cagttgtcgt
      121 accaggaatg ggggcagata tgcgtttggg cgatgtgatg caacaagagc atgcagatat
      181 gggtatctct ttttgtggta gtggtggtgc aggtgcatta acagcagcaa caaaatatgg
      241 ctatccagaa cgacatggta tgcgttcgat tgaagaaggg atcacagcta tcaaggatgg
      301 caaaactgtt ctaggtttcg gctttatgga tcaagaggaa ttagggaaac gtctgacaga
      361 agaatttatc aaaaagcatg gaaaatagaa agagggacat gaaatgagcg ttcaaaagac
      421 aattcagcaa aaagttatcg tctccggtaa aggggaaaca aagcaacatg cttttgcatc
      481 agcgttagca gaaattcaaa acaaaataat ggaacaatca aacgtattat tgcgaatcga
      541 accagtcaat gtggatattt taaaggcggt cgaaaacaaa tacacagaac gctttctatt
      601 tttcttcttc ccacggcaac gtattagcta tgaagtcgaa ctctctgtta ctgtggctgt
      661 aacagaaata aaattggcag acatatcgtt cagccagcaa atgactgaag atccaaatgg
      721 gattgccatt ccatttgtaa ctaaaaaaat ctaattttga atcataaggg ggaaagaata
      781 acgtggattt cgtaatcatt ctagtcaaat cggtcattat cgggggatta cttggttttg
      841 cagcatctgt cggggcagct cgaatgtttc atgcacctaa tacacaaggt ttaggagctt
      901 ttagaacatt aggcgaaatg aatgcctgca tgggtgatcc agcttctcat ttttcatttg
      961 gtttaggctt tttctttaat gcatgggctt cttcagttgg tgctggtgca ttcacacaag
     1021 atgtgacgca tcgaatcatt cccaattggg cagcggcagc acttctagca aaaaacaaag
     1081 atgtcaccca gaccatgcat gatccgaaaa aaatggggat cattggagcg gttatcgggg
     1141 cagtggtggt agccttctta aacacaacag ctgcagcaat tccagaggcg ttacaagtga
     1201 cggcggtttc cgttttggta ccagcagcga cgattctgat caatacagta atgccagtta
     1261 ttttttggtt ggcagcttta gacgcaggga aacgaactgg tttttggggc actctttttg
     1321 gtggtatcgc gcaattgatt atgggaaatg cggtacctgg tgttgttcta ggaatcttga
     1381 ttggtaaagg cgtcgatgag agcggttgga atcgcgtcac gaagagcatg atgatcgcta
     1441 ttgttttatt atttattttc agtggatttt tccgtggatt tgatatggaa atgatcaaga
     1501 gcttccagct tcaagtgcct aattggcttg ataacttgca caacatcttt agtgtgaaat
     1561 aggaggagaa tcatggagac aacagaagaa caagcaaaag tattagaagc aaagcggcaa
     1621 aagagctttt ggtttgcaga ctggtctttt ccgctaatcg taggaattat gtccgctgct
     1681 gtatttgcag gaacacacat gtatgtgatg tatggtgtgg gtgcattcaa tgaagtttcg
     1741 atcgtggcga tgttaaaagc cgggctggat ggcggatcct atggcgcagc ggcagcgttt
     1801 ggtgctagtt tcttatttgc cagaatttta gaaggctctt tagttgggat cttggactta
     1861 ggcggttcga tcctaactgg gatcggaatt ggggtaccag caattctctt aagtatgaaa
     1921 atagcggcac caatcgataa ttttgcactc tctttattga ctggtttggt tctaggtttg
     1981 attgttggtg gcatcattgt cttgattcgt aaatttacga tcaatcaatc aaattccact
     2041 tttggtgctg acgtcatgat gggggcagga aattcatctg gacgtttttt gggtccatta
     2101 attatattaa gcgcgtgtgc agcatctatt ccaatcggca ttggcgctac attaggagca
     2161 ctaggattct atgcttggaa aaaaccaatt gctggaggtg ctattttagg agcaatcttc
     2221 ccggcagtaa attaatagaa agagggtcat taaatgtttg atactttgat taaaaatgga
     2281 aggttagttg atggatctaa aatagaggtc gctatagaaa aaggcatgat taaagcagtt
     2341 gcagcagaaa taaatcagcc ggcgcaagaa gtagttgact tggagggaaa gtattattta
     2401 tccgctgggt ggatcgatga tcatgtccac tgctatgaaa agatgaatct ttattatgac
     2461 tatcctgatc agataggggt cgaaaaaggt gtgacgacgg tcattgatgc tggaactacg
     2521 ggagcagaaa acatccgtga attttatcaa ttgacgaaat cggtcaagac aaatgtgtat
     2581 gcgctaatga atatctctaa atggggcatt gtcaaacaag atgaacttgc tgatctaaca
     2641 aagattcaag aagatctagt aagtactgcg ttagcagaac tgccggaatt tattgtcggg
     2701 attaaagcga gaatgagtaa gacagttgtt ggtgaaaatg gcattaagcc attagaatta
     2761 gctaaaaaaa tccagacaga aaacaatgat ctgccattga tggttcatat cggttctgca
     2821 cctcctgagt tagaagaagt attgtcaaga atggaaagag gcgatgtatt gacccattgt
     2881 tttaatggaa aaccaaacgg tattttagat ccaaagacag atcaaattaa aaactttgtc
     2941 tgggatgctt acaataatgg tgtcgtcttt gatattggac atgggacgga tagctttaat
     3001 ttccatgtgg cagaaaccgc actaaaagca ggaatgaaag ctacctctat tagtacggat
     3061 atctacatcc gcaatcgtac aaatggccca gtttttgatc tagcaactac tttagaaaaa
     3121 ttgagggtag tcggctattc ttgggaagaa atcattgaaa aagttacagc agttcctgca
     3181 cggaattttc atttgaagaa taagggccag ttagctgtca attatgatgc agatttgacg
     3241 atatttgaga ttgttgaaaa tgaaaaggaa ttgaaagatt ccaacggatt tacacgtata
     3301 gctaaagaac aaattttacc gattaaaaca attattggag gcgtcatata tgacaattag
     3361 ctatggaaag tttgatttaa aagaagtcat taatgcatcg ggaaaaatga cgatcctagg
     3421 ggtttctaag gtatctgaaa gtgtcctagc agcacaacga tttggaggcg agcatttttt
     3481 tgagatgagt gatcttgcca tcaaaaccgg cgcttatctt tctcaattac tgaaagtgga
     3541 agatgcgcaa atcgtttctt ctgcttcagc agggattgct caatctgttg ctgctttaat
     3601 cggacaggtg acatgtacca tgtctatcat ccctatacag aaaaaatcgc tagacgggaa
     3661 atcatcatac ctaagggaca taatgtagat tatgggacac cggttgaagt aatggttgct
     3721 caaggtggag gaaaagtcat agaagctggc tatgcaaata tgtgtacacc ggaacatgtt
     3781 tccatgatga ttacggaaaa aactgctgcg cttctatata ttaaaagtca tcatgcagtt
     3841 cagaaaagta tgctgacggt ggcagaaatg gtagaagttg ccaaagctca tcgtttgcca
     3901 ttgatcgttg atgcagctgc ggaagaagat ttattcaaat atagcgaaat gggcgttgac
     3961 ttagtgattt actctggggc aaaagcaatc gaaggtccga gtgccggact ggtcatagga
     4021 aaaaagaagt atattcaatg gattcggcta cagtctaaag gtattggccg agcaatgaaa
     4081 atcggtaaag ataatatttt aggctttacc caggcactag aagattattt aaaaaatggg
     4141 agcgaatccg gtgactccat gaagcaacga ttagcgcctt ttgttaacgc aatcaatcaa
     4201 cttcctgacc tttcagcgaa aatagttcaa gatggtgccg gcagagatat ttatcgagca
     4261 agtgtaacga ttaatggcga taaaagtgcg aaagaagtca ttcaggaatt aaaagcacga
     4321 aatccagcag tctacacccg agaatatcaa gcaaataacg gaatcattga gttcgacatt
     4381 cgttccgtca gccaaaacga aatggaaaaa attataaaaa gattgagaga aatcatgtag
     4441 gaggctttta aattgtcagt tatcccaaat tattataaaa atcgtgtatg tttaaatgta
     4501 ttagctcatt cagttgaaaa tgcaaaagca tgctatgaag cagcggaagg gcatgtcgta
     4561 ctaggtgtgc tatcgaaaaa ttatgctagc gatgaagcag caatcgaaga tatgaaaagg
     4621 taccaagacg caaccaataa tgcgttgtca gttggattag gcgctggaga tccaaaccaa
     4681 agtcagatgg tttcgcgttt atctggggtt ttacaaccac aacatgtcaa tcaagtattt
     4741 actggcgtgg ggacttctcg tgctttattg ggccaaaatg atacagtcat caatggattg
     4801 gtttcaccaa caggtaagat cggctatgtc aatattgcta caggtccgtt aagttcacaa
     4861 gcgccagcag ctgaagtgcc tattgaaact gccattcaaa tgctgcaaga catgggaggt
     4921 acttcaatca agtatttccc gatgaaaggc ttagcacata gggaagaatt taaagcagtt
     4981 gcggaagctt gcgcaaaata tgaattctat ttagagccaa caggaggaat tgatctttca
     5041 aactttgaag aaattctgaa aattgcaatc gatgcaggtg taaaaaaagt gattcctcat
     5101 gtttatagtt caattataga taaagaaaca ggagatacgc gtataggaga cgtaaagata
     5161 ctattaagca tggtaaaaaa aattattcac taactcatta gaaaataaga agcttattcc
     5221 agaatgaaat taaatggaat aagcttttta taattaatta cggaaattgt taatgtagat
     5281 aataatttag atgtacgtaa gcagaagtac agaaatgtaa gattttccaa tattatttta
     5341 ttcaacataa taaatcgtat atcaattttt ttagcgaaat ttctatttat tccaagaatt
     5401 tcagttatta cggaatcatt ttgtgcttaa taaaaagaaa ccgtttttat atactataaa
     5461 tgtagaaagg cggtaggaaa atgaaaaaaa ttattgcaat cacatcctgt ccagttggta
     5521 ttgcccatac atacatggca gcagaaaact tagagaaagc ggggaaagca gttggcgccg
     5581 aggtaaaagt cgaaacacat ggttccatcg gtattgaaaa tgaattgact gcaagagata
     5641 tcgaagaagc agctggtgta atcatcgcag cagatactaa aatagataaa tcacgattcg
     5701 gcggtaaacc tttgataacc gtaggtgtgc aagaaggaat ccaccatgca gatgagttgg
     5761 tgagagatat tttagcagga aaagcgccag tctataaaag tacggaggca attatttctt
     5821 ccgaaaataa aagtgaatca gaaaatttag gtaaaaaaat ctataagagt ttgatgaatg
     5881 gcgtttcgta catggtacct ttcgtagtta caggagggtt attgatcgcg atttctttaa
     5941 cattaggagg aacagctaca cctgaaggga tcaagatacc agaaggaacg atttgggcaa
     6001 cgatgaatag tatcggaagt atcgctatgg ggttgatggt tccaattcta tctgcattta
     6061 ttgcgcaaag tattgcagat cgtccaggac ttgttcctgg atttgttggt gggatgttag
     6121 cggcaaacgg agcattatat ggtagtgatg cgaatgcggg attcttaggc gggatcatca
     6181 ctggattttt agcaggattc attgctttag gaatcaagaa agtgcgagta ccgaaagctt
     6241 tacaatcgat catgccaatt attgttatcc caattttagg atcattggca gttggttttg
     6301 tgtttatcta tgttattgga gaaccagtag ctcttctatt tagttcattg actcactttt
     6361 tagcagggat gcaaggagga agtgaaattg ttctagctat gatcttagga gcgatgattg
     6421 cttttgatat gggggggccg ttcaataaag tagccttttt gttcggatct ggcttgattg
     6481 cagaaggcaa ttattccatt atggggccaa ttgctgtagc tatctgtatc cctccattag
     6541 cattggggct ggcatcattg attttaaaaa ccaaatttac gaaaacagaa cgagaagctg
     6601 gtaaggcgtc cttagcaatg ggactgtttg ggattacaga aggcgctatt ccgtttgctt
     6661 caacagatcc actacgggtg attccaagca ttgtaatcgg ggcaatgaca ggaagtgtga
     6721 tcgcgatgct ttcaggagtg acagatcatg tagctcatgg cggtccgatc gtagcggttt
     6781 taggagcagt ggatcacgta gccatgtttt ttgtagcagt gattgctggt attgctgtaa
     6841 ccgtgctaat gttaagaatc cttaaaccga cattacaaca aactgcggtt gctgcagcaa
     6901 atggtgtgac agtagcagat ctatcagaga ctgaaacaag tgatgtttat acgaaaactg
     6961 aaacttttga ttctatcacg gatattatgt cagatgaagc aatcgtttta ggtgaaacag
     7021 caacaacaaa agacggagta atcgaagatt tagtccaacg tttagaaaaa acagcggcga
     7081 tcacagataa aaccagattt aaagaagtca tctatgatag agaacatgaa agtacaacgg
     7141 gaatcggtat ggggattgct attccacatg gaaaatcagc ttatgttctt aaaccaacag
     7201 tggcatttgc cagaagtcaa tctggggtag attggcattc tttagatgga aaacctgcac
     7261 atatgatctt tatgattgct gtacctgaaa acagccaagg tgatatgcac ttgaaaatac
     7321 tgcaacgtct atcacgtaaa ttgatggatg atgacttccg tcaagcactg atggaagcgc
     7381 cagataaaac aacagttcac caattattaa gcgaaatgta acataagatg aaaaatcgac
     7441 cgtgatgaga cataatagat gtgttatcat gatcgatttt ttatttagaa gggagtttct
     7501 gcacattatg aatcagcgtc agcaatcttt gatctatcaa ttaagtgaga acaataatta
     7561 tataacaaca aaaaagctag cagagcattt ccatgtgtca gaaagaacga ttcatagcga
     7621 tttgaatacc attgaaactt ggttgagaga caaacatctg cctttaatgt ttgaaagaaa
     7681 aaaaggtaca ggtatcttgt tgaatggtct gcctcacgaa agggaacaac tgaaacgagc
     7741 gcttaatgaa gagactcaaa cagaaatgga taaaaaacag cttcgtcagt tattgatttt
     7801 ccatctgcta gtagcaaaag acggatttag tcttgaagaa ctttcagaaa aactatatgt
     7861 tggaaaacgt acgattcgta aagaattgac agagttgcaa agcttttttg agtatcacca
     7921 tttacagcta gtatccaaaa caaaactagg aacttttctt aaaggcgatg aacaagaaaa
     7981 aagacagttg cttgttaaga cattacgaaa tatgcagaaa gaagatccac aaagtccgtc
     8041 gttgaaagaa ttcttcgcta aagatacatt aaaagttatc caacatacat tacaagatgt
     8101 tttttatgaa aaccatttag aacagccgaa tggactggct agtgtggaga ttcacatcta
     8161 ctttatgctc gagcgaatga aacagtacca gaaagtgaaa ttaagtaaag acgaaaacga
     8221 agtcgtggaa cacacccaag cacaaaagtt aagtagtcaa atattagcaa aactagccac
     8281 catctatcca attgaatttt ctcctgatga aatcaattat ctggctttgc gtatcgccaa
     8341 tctttttaca aattcacaag cagccacacg ttttcaaaag gaatctagcg cacttgctga
     8401 tcatttgatc gaacaagtgg aacagtttct aggctattca ttaaaagaag atcagctact
     8461 aaaacaaaat ttaaggtctc atttatcttc aacttatttt cgactccatt atggtttgca
     8521 gatttctaat ccattgacga aaaatgtgtt tagtacgtac acacaacttt tcttagtttt
     8581 acagttaatt ttagaagatt attttgaaaa ggaaaatttt tatgttccac aagaggagac
     8641 agcttattta acgatccatt tccaagcagc gatcgagcga cacaaacatc agaaaagtag
     8701 aagatatcaa acggttttag ttagtgaata ttcaaaagct atggctactt ttctcgaagc
     8761 acggctgaac agagaacttc cagaattaac gattatggat ctagttgaat ataagccaga
     8821 attaaagaga caagattttc cctctgttga ttttatcttg gctacgttac catttaaaca
     8881 tataagcatt ccagtaatcg agatctcacc aatgatcaca gaaactgatc tagcctattt
     8941 gacaaaatat atgctggagc atgtgcctat caagaagaaa aaaacatttg atctagcaag
     9001 ctttactcat ccatttctga tctttcctca acttgaacgg acagacccag ccgatatttt
     9061 aaactttatg ggaaacgtct tagtggaaca tcattatgtg gagccggaat tcatcgattc
     9121 tgtcttggaa agagacagtc actccagtac tagagtagca ccatttgtaa caatccctca
     9181 cggtaatccg ctgtatgtga agcattcgat gatttctatt gctacaatga aagaaccagt
     9241 cctatggcac ggggaacaga tacgtatcgt actgctatta gcaataaaga aaaatgattt
     9301 gaaacatgca gagttcaaaa aaatattttc tgttattcac tatctagaaa agcagccaga
     9361 acaaatggct cagttgatgc aatccaatca tccgctagaa atcttaacgt tgttatctag
     9421 ttatgaataa gtagcgatta aaaaactgcc tttatattta taataattct atctatttat
     9481 ttggaagtag aaaactaaat acaaaaaaag aggatgtggc caaagttgtg gcacatcctt
     9541 ttattttgag tttcttataa ttttttatag ttgcttacga tgtcttgact actggtaaac
     9601 ggtgttcaag agacaatttg tcggcaataa ggcacaattt tgtaaaatat ggaaagctat
     9661 ttttttaaca ttgtttctta gtgcttgaaa aatcattttg aatacttctt tatacgatcg
     9721 atgtacgttg atttaagtct aaaacttttc ttttgattcc ttctctgact tttctgaatt
     9781 ccgttaatat ttcttcttcg ctcccgacag cttgtgcagg atcttttagg tcccagtgaa
     9841 ggtgttcgtg attgtttgga acggcagggc acttgtcttt ggcatcacca caaagtgtaa
     9901 tgatcaagtc tgcctgagta aagtaatctc ta
//
LOCUS       DAYYCW010000098         9712 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000098 DAYYCW010000000
VERSION     DAYYCW010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.100"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..243)
                     /locus_tag="ACHAYA_002343"
     CDS             complement(<1..243)
                     /locus_tag="ACHAYA_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8606100.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
     gene            complement(236..1828)
                     /locus_tag="ACHAYA_002344"
     CDS             complement(236..1828)
                     /locus_tag="ACHAYA_002344"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM8606101.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(2338..3063)
                     /locus_tag="ACHAYA_002345"
     CDS             complement(2338..3063)
                     /locus_tag="ACHAYA_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606102.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(3217..3543)
                     /locus_tag="ACHAYA_002346"
     CDS             complement(3217..3543)
                     /locus_tag="ACHAYA_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606103.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(3580..3945)
                     /locus_tag="ACHAYA_002347"
     CDS             complement(3580..3945)
                     /locus_tag="ACHAYA_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM8606104.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(3982..4197)
                     /locus_tag="ACHAYA_002348"
     CDS             complement(3982..4197)
                     /locus_tag="ACHAYA_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8606105.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            4326..4661
                     /locus_tag="ACHAYA_002349"
     CDS             4326..4661
                     /locus_tag="ACHAYA_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8606106.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(4737..6734)
                     /locus_tag="ACHAYA_002350"
     CDS             complement(4737..6734)
                     /locus_tag="ACHAYA_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606107.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(6832..7107)
                     /locus_tag="ACHAYA_002351"
     CDS             complement(6832..7107)
                     /locus_tag="ACHAYA_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8606108.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            7339..8073
                     /locus_tag="ACHAYA_002352"
     CDS             7339..8073
                     /locus_tag="ACHAYA_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8606109.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            8112..9254
                     /locus_tag="ACHAYA_002353"
     CDS             8112..9254
                     /locus_tag="ACHAYA_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8606110.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         3182 a         1815 c         1421 g         3294 t
ORIGIN      
        1 gattaattca ttatattttt tttcagtaat tttatatctt gtttcgtaat tatcataaat
       61 agcattttgt tgttcataga caacaaaatt gctattagat acaaagaatt ctttctttaa
      121 ggcactccca aatggtcccc tccttactcc atctttttta tcaagtaagc attcaaactt
      181 acgctgttcc caagcttccg taaatcctgg gaaacgaatt tctggttgta tatcattact
      241 catgtttgaa cacctccagc gcaccttgta accacgcttc attttcaggt gttgtttgca
      301 gggaagagat agcttctaaa agttcttttt ctagcacttg ctgttcttgt cgtatcttct
      361 tgatgtcatt tccgacatga accatatcaa ttggttcttc ttcttcaaac gtatctacat
      421 agcgtggaat atttaagtta tagtcatttt ctttgatttc atcaaagttt gccaaatgag
      481 cgtatttctc gacatctttt ctttcagcat aggtttcgag gattttttga atgttttctt
      541 ccgataattt attctgattt tttcctttga caaattctcg actagcgtca atgaataata
      601 cgtctcgagt ttggcggttc tttttcaata caatcaccgt cgttggaatc gatgtcccga
      661 agaacaagtt cgcaggcata ccgatcacag cataaatact gccgtcttct agtaattttt
      721 gacgaatgac cccttctgca gcgccacgga ataaaactcc gtgtggcaag acgatggcca
      781 tagtccctgt ttccttcaaa tgatagaaac catgaagaag aaacgcaaaa tctgctttgg
      841 attttggcgc taattttcca taacgattaa accgagaatc gtctaagaag gtcgtatctg
      901 ctgaccaatt agccgaataa ggtgggttca ttactactgc atcaaatgtg taaggttcat
      961 ctgttggcca atccttattc aacgtatccc cattgcggag attcatttct tcggcatcta
     1021 ccccatgtaa aatcaagttc atttttgcta gattgtatgt cgttgtattt aattcttgcc
     1081 catgatattt aacgttatct gggtgagtta agtagttacg cacatttaac atcaatgaac
     1141 ctgaacccat ggttggatca aaaacactaa agaatcgtcg ttctttttga tccaacgtca
     1201 cgatttgtgc catcatatca gaaaccatat gaggcgtgta aaattctccg gctttcttcc
     1261 ctgcttctga agcaaattga ctgatcagaa attcataagc atccccgatc acgtccccat
     1321 cgtgacctaa aacttccacg tcattcaatt tcttaatgac ttcggtaatc gtcacatttc
     1381 gttgttgctc atctgttcct agtttttttg attggagatc cacatcatca aataacccat
     1441 taaattgatt gtaggtagat gccaattgca caaaggcttt gtttagatca ttcaattgga
     1501 acgttgcttg ttttgcttga tccgctaaga cgttgaaaag atactctggc gaaatcgcat
     1561 accccaaaat atcaacgatt gttgcaatca gatcttcttt tgattcttca tctgaaagta
     1621 attcgcgata caacatcgtt tgtttgctta ccgtatcata ttcctctaag gattcatctg
     1681 ctaaaagaac cacttgttcc aacaaacggt cggataaata tttatagaaa atcagtccca
     1741 ataaataatt tttatattct gacgcatcca ttttgctacg gagattatct gccgcactaa
     1801 ataatttttg atttaattct gcacccaaaa agatacactc ctatctctta tttaaagttg
     1861 ctatataccc tcttatttta gcatactccc ttttagaaaa agcaaaaaaa aaaagaaaga
     1921 aacttattct tgctaataaa ctacgcgaaa ggagtctaac gttgtttgat ataacttaga
     1981 ctgatcgtta acttattttt tatatttcta catagttatc tttagaacca tttcagatac
     2041 atatgaaaca attacatatg attttttcat aaaaaaacag tactgtctag tagtataaaa
     2101 tatacaaatt tcttaaaaat tttttcacta aaatcactac ctgaaacagt ctaaaacact
     2161 tatatatcaa cgtttaatga aactatctcc gtttcatttc agatacaaat aaatatagtc
     2221 gggctccaaa tccgaaaaaa aataccccca gtagcttatc ctaagctact ggagatgtat
     2281 aagtacggtc atataacaac ggggtagggt aaataaatga aaaagatgga agaatcatta
     2341 ttcaataact tttttaaata cttcgttgta atcagttacc tgtccgttct taaataccac
     2401 agaataatct tttttcccat tttcaagtaa ccgaagtgga atatctttac caactttctc
     2461 tgtcactgca ttactgatac ctgttagatt agctaataaa tctgataaag attgagcatc
     2521 tgtagaaatc tctgtatcag tatttatcgt gattccttta tcttcattta caagtttcca
     2581 agtaggtggt atagctgtgt atgtttctac aaatgaatta agtttttcat taagtgactt
     2641 ctcttttata tttccatcta aaaactctgt atatatggct tgagctatac taaaacaaag
     2701 ctggccttgt tctaataatt ttacatcatt agttagtaaa gctgtgttca cgttatcaat
     2761 cgcttcaaga agagtatctt tggtgtccct aacagcttta ggtgtgcctt ctggaatttc
     2821 cctacttgat acctcgtcca ttttatcttc cataaacccc ttaaaatcat atctttctcc
     2881 caaatccata gaagtttttg agaaagcttt catttctgag tctatttctt caataatatt
     2941 taacatgtct gatacatatt gtttaggctg attcgttttt aatctatatg tggtaaatat
     3001 ctcaattcct ccaattatca ataaaattgc caaacaaatc cataataatt ttttcttttt
     3061 catttttcct cctatacact gctgttataa cagtgactaa tgtaaagatt ttcccagcaa
     3121 tcatcagact attatttaca gggaatcaaa aaaactcctt agtaagctta cacctttagg
     3181 agttcctgtt tgaacggtat gcataataca tttaaatcaa tttaacatta acacctcagt
     3241 atatggtatc tctacatata cacggtcttt ttttggatag tactcgacat ttatattgat
     3301 agcacttcca ttgtctaacg tagctttagc ccggtacgtg ttatcaccat tatcatgagt
     3361 gatcgtcaca tcatcacatg tccgatttaa gttatactgt tcttgtaaaa tctgagttac
     3421 caatggtaca gatgacttct ctagtccaga tgaacccgtc attcttgaaa aaattatacc
     3481 tataatagcc aatacaacga tacctataat gacaaacttc ttattcatat ttactttatt
     3541 caacttttta tctcctatct ccaaaatgat tttctctcat cagtaactca ttgacatccc
     3601 caatagtaca ccaaagatgg ctccagctac ccctgctatc atcaaaatcg tacccatttg
     3661 ttcatctttc tctcgatata agtaacccat aataacagca acagcaccaa ataccacagg
     3721 aataaatgct aatgatacta ccatagaaat ccaaccaata atcaaataca gcttccctgt
     3781 ctcttgtcca tgtacgattt gttccttcat ttcattaatt gtctgctcag tacttttctt
     3841 tactgtgtct gttccggttg tctcatctgt ttcttgtaat gctccgcaaa actggcaata
     3901 cttactttca tttgttattt ctttaccaca ttttctacaa tacatgtctt ctcctcctaa
     3961 aatcattcaa ttgcttatta gctaactgtt tactttatta aatacatcag aaaacagctc
     4021 atttattggt acacttaata tctcagaaag cattttcatt tgatagggtt taggaatcgt
     4081 tctccctgtt tgccacgaac ttacacattg ctgtgtaaca ttcatttttt tggctaactc
     4141 agtttgggaa agtccttttt cttttctgta tcggctgaga ttacttactt ttttcatgat
     4201 tctcccctcc tgttttatta tatacaacaa tatgatgtat ttcaactcat aaaatatata
     4261 aatacttcaa atgtcattac aaatacaaca acttgttgta ctctactgag aaggggggtg
     4321 tattaatgaa acagaagcaa tatgcaaagg tcattagaga aaagcgaaaa gcactcgggt
     4381 ggacacaaaa agaattagct aaaaaaatct tctctacaca acaggcagta gcaagatggg
     4441 aaaattctgt aacagaacct aacctcgata gcttaacagc tctttctaga gccctaggaa
     4501 cccctgtaag ccattttcta gacaatgttg acatagatta tgaggaagag tttttggctc
     4561 tctatcgctc tcttagtact gaagatgcaa ttcgaacaat tgactatatg aagctattaa
     4621 aaaggcaaga aaatgaacgc aatcagctat tgaaagattg aaaaataaat aacccaccga
     4681 actttaacgg tgggtgtatt gagatgaaat aagattaaga gctatggata acctgtctag
     4741 tttaatataa ttgggtctct tctattcctt actctccgtc catcatctct tgttacactt
     4801 gtaaattgat aaataggaac taattcaact cctgataata tcaattcttt tattacttca
     4861 tctatccctg cccctttcgc tagatatact aaaaaactac tgcttgcttt cacattgtat
     4921 agtttcttaa tatccttaga agctcgttca agagtgaatc cttgtttaac aaagtagcct
     4981 ctgttttcta ttaaagcatt tttcacgctg ttatccctga catttcgtag gcgttcacaa
     5041 acagtgttaa taagttcttt ttctgtcatt agatatgtcc cttgccacga taagccctgt
     5101 aaaacttcat gtgcttcatc taaaatcaat tgtttttcct tgctaagttc tgatcgggaa
     5161 ttttgaggaa atactctttt agcctctttt tcgttaaaac atatcttaac ccattcataa
     5221 gaaaaatatt gatatgtaaa atgaaatttt tccagaagtg gaaggatatc ttcattcaat
     5281 ttttgaatca cagaagttgc attaaaaacg tctaatcgat agacattgct tctcttatgt
     5341 ttaaagtttc ctaacgcttt ctcttccttt aacctattta ggtattcgag catcttcaaa
     5401 ggaacatctt catcatctaa tttttttatc aatcctataa aagtcataag tgatagtaac
     5461 ttattacatt tatctatgtc ataagtttta ccatagtaag aactcatttt tttagctaat
     5521 tgttcccccg acaaaataca agtcacactt tccttactat catcaaaaaa attaacatca
     5581 gaaagcaaga catcatagac acgtcgcatt tcctctagat aattccttat tgtcctgaaa
     5641 agtttgtctt tcattgattc aagagtgtca aaaagtactt ttgattcctc tttcaatttt
     5701 cgaaaatgtg taggatacaa gtctaggttc tctaatagcc aattaaatgt ttctacccga
     5761 ctgtctttct ttaaaattag catcacaact ccaataaaat tttcactgat atcacaatta
     5821 ctggaatgac aataaaaaat ggaatgccca gttttactgg taaaaatact agcagaggga
     5881 ttatgatcgt tgtgaaacag acatcttaag tttttacttg acttaagtaa tttattcata
     5941 tcaatggtta acaatctttc ataaatctca ttaaaagaca tgtcggagga atcaaacagg
     6001 cagtctttaa accactgttt catttcttct ttgtcgtcat tttttatcaa cttaacaaaa
     6061 tcggtttcat tgactagttc atactctctc cctgaatcac tttgacaatt tttagaacat
     6121 gtcatggtaa aatcaatcgg aagctcagta atatctaaca tgttgtcttc attaattatt
     6181 atcactgcat ctgacttata tcctccaaaa aacattctat tcgaagcatt tacattgtca
     6241 tcacacccgg ggatgttctt tttcaagtat tcgtaaacag caaccatttt accatagtca
     6301 gtaattgctt cttttaatag aaagacaatc ttgtattttt ctagattttc agagtaccga
     6361 atactttttt gtatcgcaaa cgcagaatta tgaactaaat tgatagataa aaccttttct
     6421 atgcttaagt atctactatc atctttactc aaatctacta ccttcccctt ctcatcttta
     6481 acagtgttat cgacatccag aataattact ctggtttcca caaaactctc cttgtcattg
     6541 cctttattct ttgtcttgct tggaataaat gattgtgatg aatttagtat ctctacaaac
     6601 tcatctcttg taacttctct atagttatct aaacacctat ctcttacatc agttttttct
     6661 ttttttgtaa ctggcttctc taataatttt tctttatcta cataaattgg aatattaatc
     6721 attgatgttt ccatttgtgt ttcctcctta taaaattctg ttaaagacta aaacatacag
     6781 ataataaaaa caaactcttt attactatat gttttagtct tatttgttta ctcaaccttt
     6841 ataccgttcc atgaaactat caatatcaga ttttttaaat ctaatgaccg tatcaatttt
     6901 tataacaggt aacccgtatt gagaaacata ttttctaatc gttcctactg atacacctag
     6961 atattcactt gcctgctgat agtccatata ctctagagtg actccagaaa taatgtcttc
     7021 tagcataggc tttagcactg taattatctc tttcacttgt tgttcaactt gtatagaaac
     7081 aatgctatca atgatttgta gagacatatt tccccctcct ttcgtgattg cttgaacaaa
     7141 ttttctaaga attatcttcg tattttttgc tcaaaataaa agtattttgt tattaaccga
     7201 ttaatcaaac aaaaaatgat taccagttca tcgacataca cataatacct cgataaaaaa
     7261 ggggtgtcaa tagttttgta aaatttattt acaacacccc ttttttgagg tatactaatt
     7321 ttaggaggtg tggtcaatat gataaaacgt ccaccaataa attatttaga acgaaaaaag
     7381 atactcggaa caaaaataaa agctataaga aagagtaaaa aattaacaca accagcattt
     7441 ggtcttatga ttaacaacgg tcaattaatt gacaaaaaga ctatctacga atgggagaag
     7501 ggaacctatt taccaatacc agagcgatta tcccgaattg ctgatttggg aaatatgagt
     7561 atagaggagt tagtttgtgg caatgtagaa gaatatattt tgggaattat tctttataga
     7621 gatagcattg tactagatgg tataacattt ccagataaaa acctgttcca gcatcttcgt
     7681 caacagttcc caccagtaca ctctaattta gatacatggc tggatagata ttccaaatta
     7741 gaaccagaga tgcaagaatt tattgctaac aagacttgta ataaagttaa aaatgaaaaa
     7801 attagtcttt tcaatatctt aaaaatagaa gaattattca tcaatgctat tgtagaagaa
     7861 tttgacaata acattctttt cctcactagc agtatagaag aactactaga aagaatggta
     7921 gatgaatggc tacctattca attgaaagac atgtcttatc ccgaagaagc tgtgagagaa
     7981 attactgata atataaacaa gctagaacaa acgatttcat caattgggaa aaaatacact
     8041 aagaaaaaaa tgaagggagg tgataccatc tagtggtaag tactccttgt aaactaagag
     8101 gagatgattt catggcaaaa atatcaaagt acaaaaagaa aaacggtcaa gtggcgtgga
     8161 tgtataaagg acacattgcc accgatccac gaactggtga aaaaataaat actacgagaa
     8221 gaggttttag tagcaaacaa gaagctcaaa aagattatga cgagtacaga catcaaatat
     8281 tatacggtgt taaaaagaaa gcacctgata tgacatttga agacctgtat aatgaatgga
     8341 taactcatca gcgtaccagt gttaaagctt caacaatcgc aatctcagta agatacgcaa
     8401 ataatcaaat acttccagca tttggtaagc ttaaactatc gaatatctct gtcccatatt
     8461 gtcaaaaagt ggtagatgaa tggcacagta aatatgaaag ttacgattat atgagaaaac
     8521 agactgcaca aattttacgc tacggggttg caatgcaata tattgacaat aatcctatgg
     8581 aaaaaacact tcttccacga aaaaaagagt atgagaaaaa tcgtaaattt tactcaaaag
     8641 aggagcttaa taatttactg gatgctttta aagattttgg taacatgaaa caatacgcct
     8701 tcttcagatt attggcatac acaggtatga gaaagagtga agtactcgct ttacagtgga
     8761 aagacataga cacgtttaat aaagaactac atgtcaataa aactcttgct gtagatgaat
     8821 ttggtaaagt tataattcaa tctccgaaaa caagagcttc tagacgagtt atctctttag
     8881 atactgagac tctttctatc ctcaacaact ggaaattaca gcaaaaagaa gagtatttaa
     8941 aacttggata caatactagc tctaaagagc aacatgtttt tacaacagtt aaaaacactc
     9001 tatacattcc aaacactgtt aatgattggt taagatatat tttgaaaaaa tataacttgc
     9061 ctcgtattac acctcatggg tttagacata cacatgctag tttattatta gaagcaggag
     9121 aatctgtcaa ggtagttcaa caacgattag ggcatgaaaa tagtaaagtg acattagaca
     9181 tctatgcaca tattacaaat aatgcaccta agaaaacagg tcaagacttt gctgatatga
     9241 tggctcatca gtaaaagagc tggtcaaaat ctggtcattt ctagtataca ggcaaaacaa
     9301 aaaagctaga acccttatat atcaagggtt ctagctccta taacaggttt acccaacact
     9361 gccttccaat gactcttttg ggaatcagag gaaagaatca ggctttatag agatatttca
     9421 tctaacaaat tactttagac tgacagtata tatcagcagg tactgtcatc gggaacgtag
     9481 ccaatatcgt atccaaaaca gcaggtagag aagctatttg aggattcaag ttattctatt
     9541 tcaaccatga agttaagttc attaaaaata aagatagttg gtatttcaat aatttttgta
     9601 tagagttaat cactttattg atgctaattc atcttgattg gtattataat cgtattcaag
     9661 ttgcgtggcg tgcttttgct agaaaatata taactgttct ttataaagaa at
//
LOCUS       DAYYCW010000099         9626 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000099 DAYYCW010000000
VERSION     DAYYCW010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.101"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            211..1203
                     /locus_tag="ACHAYA_002354"
     CDS             211..1203
                     /locus_tag="ACHAYA_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8606111.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            1219..2103
                     /locus_tag="ACHAYA_002355"
     CDS             1219..2103
                     /locus_tag="ACHAYA_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8606112.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            2152..3759
                     /locus_tag="ACHAYA_002356"
     CDS             2152..3759
                     /locus_tag="ACHAYA_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8606113.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            3760..5244
                     /locus_tag="ACHAYA_002357"
     CDS             3760..5244
                     /locus_tag="ACHAYA_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFM8606114.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            5421..6413
                     /locus_tag="ACHAYA_002358"
     CDS             5421..6413
                     /locus_tag="ACHAYA_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8606115.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            6465..7334
                     /locus_tag="ACHAYA_002359"
     CDS             6465..7334
                     /locus_tag="ACHAYA_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8606116.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(7754..9367)
                     /gene="guaA"
                     /locus_tag="ACHAYA_002360"
     CDS             complement(7754..9367)
                     /gene="guaA"
                     /locus_tag="ACHAYA_002360"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8606117.1"
                     /translation="MRRTERAAYRTKFYSNLSSPTRFDEEPKIIVLDYGSQYNQLITR
                     RIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVL
                     GICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVT
                     QAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTG
                     DWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVL
                     LRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDE
                     ATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLF
                     KDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANA
                     GLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKI
                     SVRIVNEVAHVNRIVYDITSKPPATVEWE"
     gene            complement(9374..>9626)
                     /locus_tag="ACHAYA_002361"
     CDS             complement(9374..>9626)
                     /locus_tag="ACHAYA_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8606118.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         2999 a         1631 c         2090 g         2906 t
ORIGIN      
        1 gaggccgcag aaaagggtgc ttaaaaataa tctgttgttt aaaatattat tatcagaata
       61 cttagataca aaattctggc acaaagtaaa aaaaatattg acttaaaaaa atggatatgg
      121 taatctataa acgaaaatag tggtaacgtt tccaaaatgc atcttcgtta aatttttatt
      181 tttttaaacg aaaatggaaa cgtttctaaa atgtattggg aggagattga attggaaaag
      241 acattgggac caggtggtga gataagggcg gtacaacggg ataaaaaaaa tatttcgaag
      301 tgggaaaaat taaagaaaca gttgcggcgt gataagtgga tgtacttgtt gctcttacca
      361 ggtatcatca tgacgtttat ctttaagtat gtgccaatgt acggtgctgt gattgcattt
      421 aaagactata atcctatgct aggaattttg gaaagtcctt gggtaggact agatcacttt
      481 agggaattta tcagttcacc aaattttacg cttttgctga gcaacacatt aaaaattagt
      541 atttatggtt tattgttagg tttttttcca ccaattattt tagcactgag cttgaatcaa
      601 attatcaata aaaaaataaa acagaaaatc cagttgattc tatatgcgcc gaattttatc
      661 tcagtagtta ttattgtggg gatgctgttt ttgttcctat ccgcaaatgg acctattaat
      721 gggttagtag aaaaggttac ggggcaacca attatgttca tgtctaatcc agcttatttc
      781 agaacgattt atattttatc gggaatttgg caaggaatcg gctggtcatc cattctttac
      841 accgcaacgc tggcgggagt cagtaccgat ttatatgagg cagcaaatat cgatggggca
      901 aatattttac aaaagatttg gcatatcgat ttgcctgcat tgaggcctgt gatggtgatc
      961 agcttcatct tggccgccgg cggtattatg aatgtaggat atgaaaaagc ctacttgatg
     1021 caaacttcaa tgaacatccc tgcctcagaa atcatttcca cttatgtata caaagtcggc
     1081 ttgcaatcag gtgattatgc ctactctgct gctgttggtc tgttcaatat gattattaat
     1141 gtcgttttat tactgattgt caacaatata accaagcggc taaacgatgg tcaaggcttg
     1201 taatgaaagg agaaaattat gaatagtgct ttatcaaaaa tcgaccgtca aatcttatgg
     1261 ttgaatcgag tgattgtaac ttttttagtc gtcatcacca ttgttccgct gatttatgta
     1321 ctggtagctt cttttatgga cccagctact ttgatcaata aaggtataag cttcaatccg
     1381 aaagattgga caatacaggg atatcaacgg gtatttgcag atgattccat tttacgcggt
     1441 tttgtaaatt cgttgtttta ctcttttagc ttcagcttat tgactgtatt tattacgatg
     1501 atcactgcgt atcctttgtc tcgacctaac ttggcgggga aaaagattat tatgactttt
     1561 tttgtcatta caatgttcgt gggtggtggc ttgataccta cctatttatt ggtaaagaac
     1621 ttgggcatgt tagatagtgt atgggcgatt atcgtaccag gtgctataaa tgtgtggaat
     1681 attatcttag cccgtaccta ttttagtgct ttgccaaaag aactttctga agctgctact
     1741 atcgatggcg ccaatgactt gcaaatactg ttcaaagtca tgcttccatt agcgaaacca
     1801 attatgttcg tattgttttt atatgccttt gttgggcaat ggaactcata ctttgatgcc
     1861 atgatttaca tcaaagatcc cgacttacag ccgttgcaac tcgtattaag aaatattttg
     1921 atccaaaacc aaccttcaca agacatggtg ggagccaata ctgccatggc agaaatgaaa
     1981 caaattgctg agttgatcaa atacgcaacg atcgtcattt ctagtctgcc attaatcata
     2041 atgtatcctt tcttccaaaa atattttgat aaaggtgttt tagttggttc tgttaaaggg
     2101 taagtattgt tcactatttg tatgaataaa aattataaag gagattttaa gatgaaagca
     2161 aaatggatgc tgccaatcgt ttttacttcc gtactaattt tgggggcttg tggaggctca
     2221 aaagaaaaga gtaaatcaag ccctgattat gaactagaaa atgtctcttt tccattgaaa
     2281 gaaaaagtat ctttaaaaat gatgtctcaa agtgcccctt tggctcccaa tgatcctaat
     2341 gacaaattga ttttccaacg attagaagaa gagaccaatc tccatattga ttggacaaac
     2401 tacagtagtg attttgccga aaaaagaaat ttggatatcg ctagtggtga tttaccagat
     2461 gccatcttca acgccgctgc aggtgactat gatttattaa actgggcgga aagtggcgtt
     2521 attgtaccga ttgaagattt gatcgaagat tatatgccaa atctgaagaa aatttttgat
     2581 gaaaaccccg aataccgaca aatgtctact gcacctgatg gtcacattta ctctatgcct
     2641 tggattgaag aattaggcga aggaaaagag tcgattcata cagtaaatgg gatggcttgg
     2701 atcaacaagg agtggttgga taatttgggt cttgaaatgc cgcaaaccac tgatcagttg
     2761 atgactgtgt tggaggcttt caagacgcaa gatccaaatg gtaatggtga agcagatgaa
     2821 attcctttta gctttattaa caatggagga aacgaagact taaaattttt aatgggggca
     2881 ttcggtctag gtgataatga tgaccactta gtcgttgata acgatggtca ggtacaattt
     2941 actgctaaca aagaagggta taaagaagcc atcaaatatt tcaatgaaat gaataataaa
     3001 ggtcttattg ataaagaggc tttcgaacaa gattggaata cgtatgttgc caaagggaaa
     3061 gaacataagt atggcgtgta ctttacttgg gataaagcaa atatcacggg tgcagatgat
     3121 agttatgacg tcttgccagt cttagcggga ccagatggaa cgaaacatgt tactcgtacg
     3181 aatggcatgg gtttctcaag agatcgtttc gtaattacta gtgcgaataa aaacttggaa
     3241 cttacagcta ggtggataga taaaatgtat gaaccgttac agtctgtcca aaataactgg
     3301 ggaacctacg gtgatgaagc acaggcgaat atttttgaat taaaggatgg tatgttaaaa
     3361 catttgcctt tagaggggac agcaccgggg gaactaagac aaaaaaccga agctggagga
     3421 ccattagcag ttcttgattc ttattacggc acagtcacta ctatgccaga tgatgcgaag
     3481 tggcgtttag atttgatgca tgacacgtat ttgccatata ttacagaaga caatatctat
     3541 ccacgtgtct ttttagaaaa agaagaaaca gatcgattag caaaaatcga aacggatatg
     3601 tgggattaca tctatcgcaa aagagcagag tggatcaccg gcggtaaaat cgatgaagaa
     3661 tgggaagaat accttgctga attggaacgt gtaggtttgg aagaatggat tcaaataaaa
     3721 caaactggct atgataattt tttgaaggga gcacaataag tgttagtaga agaaacggcg
     3781 attttggata aagcaaatcg atttattgaa atgatgcgag gaaaagttaa ccctcagtat
     3841 cgacaaaact atcatttttc cgcacccatc gggtggataa atgaccccaa tgggtttata
     3901 tattacaaag ggcattacca tctcttctat caatactatc cttacgatag tcgatggggg
     3961 cccatgcatt gggggcatgc gcgcagtagt gatttggtcc attgggaaga tctaccagta
     4021 gcgttagccc cagatcaacc ttatgacagc ggcgggtgtt tctcaggaag tgcaattgaa
     4081 agagacggaa aactatattt attttataca ggctgtcaag aaattgatgg tattagccat
     4141 cagcatcaat gtttggcggt ctctgaagat ggtattcatt ttagcaaata cagtggtaat
     4201 cctgtggtag cagaaaaaca gctaggtgaa aatgggctaa tacaagaatt tcgtgatcct
     4261 aaagtaattg ttcacaacga tggttactat atggtagtag ctactaaatc tacacagtct
     4321 actggtagaa tagtgatgtt tcaatcgtca gatttgtata cttggcactt ttactctgtg
     4381 ctgctagaag ggacttcaga gcaaggaata atgtgggaat gtcctgattt attccattta
     4441 gacggacgag atgtcttaat catgtctccg atacaaatca agaagaacgg actggaatat
     4501 cataatatca gttctaccat ggcttgtatt ggtgaggtaa actgggaaac aggtcagatg
     4561 atagttgata atttccatga aatggatttt ggaatggatt tttatgcacc acaaacaaca
     4621 agagatccta atggtggacg ggtgatgatt gcttggatgc agatgtggga tcgtacattg
     4681 cctacacatg agttaggcca tctttgggca ggctctatga gtttgccccg agagttaaga
     4741 gtgaaaaaaa atcgattggt acaaaaaccc atctcaactg tatatagtca tttagtttat
     4801 caacaaggca gtgaaaatat tgcagtgact gacacatcag tcgtatttcg aaacgcgctg
     4861 tctgattctt catatttaca tctagtcgca gatttggaga aagctgaagt ttttacaatt
     4921 gaattactgc ggtctaagac ggattgtctc aaactagcct atgacactgc tacacaggtt
     4981 tttgaattgg atcgtcagaa actaggaaga gagcttacag gggcggaaga agaccctctg
     5041 aatgtacgaa aagttgaagt acctttgatt gataatcagc tcgttcttga gatttttaga
     5101 gatacctcat caattgaagt ctttattaat gggttagaag ttatgacggc tactttctat
     5161 gagctggaaa aagctcgaga tatcttgttt cattcgatcg gagaaagtcg tattcaagct
     5221 tttgaaacag cgagtgtgaa ataaatgttt gattgcttag gcgatgaatc aattatttaa
     5281 taggttttga aggataatgt ttttagaaaa gaacagatag ctagatttcc catggatttg
     5341 ctattagtat tttgaaaaaa ttagtaattt atgacaaaat tcattatact aaacttgtgc
     5401 agaaatgtga gggatcaact atgaaagcaa caatgaaaga tgtcgcaaaa ttagcaggtg
     5461 ttggagtagg aactgtttcc cgggtattga ataacggttc tgttaaagct tctactagag
     5521 aaaaagttga agcagcaatg acagagttaa actatcaacc agatttttat gcccgggggt
     5581 taaagatgaa gcatacgaac acggttgctt tgattctacc cacggtatgg catccatttt
     5641 ttggcgaatt tgcctaccat gtagaaacag cattagaaag acaaaactac aagctttatt
     5701 tgtgtaactc cagtggtaat agtgaaaaag aacaagaata tatccagatg gtcagccaaa
     5761 ataaagtaga cggcatcatt ggaattactt attcagatat cgatgaatac atttcgagta
     5821 atctgccttt tgttagtatt gaccgttatt tcactgaaga ggtgatttat gtgaccgctg
     5881 acaatgcggc cggtggacgt ttggctgcaa atgaacttta tcgccgagga tgtcagaatg
     5941 tagcatatat cggtgggtac caagaaacac cgaatgaaac gaagagtcgt cgaaaatatt
     6001 ttgaaatcga gtgccgtcaa ctggggattt cctataaagc gttagatatg ttagaacctt
     6061 tagaccatct ctcagaacgt gtaacaaatt ttttgttaga gaatcccgat atcgatggga
     6121 tttttacgat caacgatcca atggcattag ctgtcattga tatgcttgaa aaaataggga
     6181 aaaacgtacc agaggatgtc caagttatcg gatttgatgg tcaaaagatg acagaaggag
     6241 atccttatct agtctcaacg attgcccaac ctacaaagca aatggcggaa gtggcagtgg
     6301 caaaattaat tgctgtcatt aatggagaga cagtagaaaa acgtaccgta ttgccgattg
     6361 aatttggcga aggtgacaca acgaaacctt tagtgagaaa cgattttgct tagtgcaaat
     6421 acgtttcttt ttttgaaaat gagtgaatga aaggtgtaga taaaatgtta tatggtggaa
     6481 tagaagccgg tggaacaaag tttgtctgtg ggattggtaa tgaacaatta caagtgatag
     6541 aacggaaaag ttttcctacc acaacaccag aagaaacgat ggagcaagtg tttgatttct
     6601 ttgataagta caaaggaaat atcgcatcaa ttggtgtagg agcatttggg cctattgata
     6661 ttcaaatcga atcaagaact tacggatata tcaccaacac accgaagtta gcttggcaaa
     6721 attatcattt tgtaggagct ttggaaaacg ccttgtcggt tcccatctat tggactaccg
     6781 atgtaaatgc tgcttgttat ggagaatata tggcagggta tggaaaaggt aaaaatagtg
     6841 ttctgtatta caccgttggt acaggtgttg gtggtggtgc tgtagtggat gggaaaatag
     6901 tcgcaggttt tagtcatcct gaaatgggac atatgctctt acaacgtcat aatgaagatc
     6961 aatttgaagg aatctgtcct tttcatcatg attgtttgga agggttagca tcaggacctg
     7021 cgattgaaaa acgctttgga agaaaagccc agactatctc atttgatcat ccttattgga
     7081 cgattgaggc agattatctt gctcagtgtg tgtataacac aacgttacta tatgcaccgg
     7141 aagtaataat tttaggcggg ggtgtaatga aacagccgca actactcgag ttgattcgcc
     7201 aatcgtttaa agaaaaaatg ggttcctacg tggatacgcc tactctcgaa gaatatctac
     7261 taactccaag gcttgctgac aatgcgggct tgattggctg cctagcttta gcaataaaga
     7321 aactcaatca ttagaatagt atttattaga actattcata acataccgga agtttaaaag
     7381 ggtattctga aacttaaata gtctcaattc attcttcttt cttgagaatc tgagtagaga
     7441 cttttattct gtttcaaatt aatataatta cgaaatgata ttgagtacaa gaaagttctt
     7501 tttaccagat cttttaaatc gaaaaatctg atatatatat ttatagtact aaaaatctga
     7561 ttagataaaa agctaattca ataggaaaat aaaggtagtg aataaaagca aagcccttgt
     7621 tagtagttaa tctaacaagg gctttggtag ggaaaatggt aggaaaccat cttatttttt
     7681 acgaaaatca ggaactttag taattgaata atgttgattt ataggtattc ctcatccata
     7741 ttatgttggc tctttattcc cactccactg ttgcaggtgg tttgctggta atatcataca
     7801 cgatacggtt aacgtgtgcg acttcattta cgatacggac actgattttt tgcaagactt
     7861 cccaagggat acgggcaaag tcagcggtca tgccgtcaat agaagtgaca gcacggatac
     7921 ctacagtgta gtcatacgta cggccatctc ccataacccc aacagagcga atgcctggta
     7981 ggacggtgaa gtattgccag atgtcgcgat ctaaaccggc attggcgatt tcttcgcgca
     8041 agatagcgtc tgattcacgg acgatttcta atttttcttc tgtcaattca ccaaggacac
     8101 gaatacctaa gcctggtcca gggaatggtt ggcgccaaac gatgctgtca ggcatgccta
     8161 attgggtacc taattcacgt acttcgtctt tgaacaatgt gttcaatggc tcgatcagct
     8221 tgaattgcat gtcttcaggt aatccaccta cattgtgatg tgatttgatg gtttgggcag
     8281 tttcagttcc tgattcgatc acatcggtat aaagggttcc ttgtgcaagg aaagaaacgc
     8341 cttcttcacc ggcaagtttg gttgcttcat catcgaagac atatacaaat tcatttccaa
     8401 tgattttacg tttttcttct ggatcagaca cgccagcaag ttttgataag aaacgttctt
     8461 tggcatcgac ttttataatg ttcaagccaa attttccgcc aagactttcc ataacttgtt
     8521 ctgcttcacc tttacgcaac aatacgtgat caacgaagat acatgttaat tgatcgccga
     8581 tcgctttttg taaaagaaca ccaacaacag aggaatctac tccgcctgaa aggcctaata
     8641 aaactttttt gtcaccgact tgttcacgaa tttttgcgat ctccatgtcg atgaagttat
     8701 ccattgtcca gtcaccagta cattggcaga catctagtgt aaaatggcgc aacaagtcat
     8761 ttccatattc ggaatggcga acttctgcat ggaattggat tccgtacatt ttgcgttctg
     8821 cattttggat cgatgcgatg gggcaatctt tgctggtagc aacgacatcg aaatcagtcg
     8881 gtgcttgggt aactaggtca ccatgactca tccaaacggt ttgtttttca ggagtgtttt
     8941 ggaaaagggc cgcttctgca gaagtgattt ccaattctgc ttttccgtat tcacggtttt
     9001 tggctggttt aacttttccg cctaatttat aagtaatcaa ctgcataccg taacagattc
     9061 ctaaaactgg gatccccaaa tcaaaaatct tttcatcaat accaaaggcc ttctcatcat
     9121 aaacactgtt tggtccgcca gaaaggatga ttcctttagg gttcattgcc tttacttctt
     9181 ctgcggtaat gcggtggctc atcaactctg agaaaacacc gaactcacga atacggcgag
     9241 tgatcagttg gttatactga ctaccgtaat caagtacgat aatttttggc tcttcgtcaa
     9301 atcgtgttgg tgaagacaaa tttgaataaa acttagtccg atatgcggcg cgttctgtgc
     9361 gccgcatatt tttttagttt tcaaagatca gaccttgttg taaatagatc cgtgcccgaa
     9421 agttataaaa gttgcgataa ccaaacgcaa ttcgtttaat cagcttgatc ttgttattca
     9481 gcccctcagt gacgccgttt gagtgggaca ccttaaaggc atttgaaata cccgattggt
     9541 agcgatggaa gatcgtgaat ttctttttaa acgactgagg taacgtagat gaaattgttt
     9601 taagggtttc ttgaaagcct tccgta
//
LOCUS       DAYYCW010000100         9475 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000100 DAYYCW010000000
VERSION     DAYYCW010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.102"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..907
                     /gene="gnd"
                     /locus_tag="ACHAYA_002362"
     CDS             5..907
                     /gene="gnd"
                     /locus_tag="ACHAYA_002362"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM8606119.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            909..1910
                     /locus_tag="ACHAYA_002363"
     CDS             909..1910
                     /locus_tag="ACHAYA_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM8606120.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1907..2191
                     /locus_tag="ACHAYA_002364"
     CDS             1907..2191
                     /locus_tag="ACHAYA_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM8606121.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2224..3021
                     /locus_tag="ACHAYA_002365"
     CDS             2224..3021
                     /locus_tag="ACHAYA_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8606122.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3035..3868
                     /locus_tag="ACHAYA_002366"
     CDS             3035..3868
                     /locus_tag="ACHAYA_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8606123.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3886..4743
                     /locus_tag="ACHAYA_002367"
     CDS             3886..4743
                     /locus_tag="ACHAYA_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8606124.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4765..5274
                     /locus_tag="ACHAYA_002368"
     CDS             4765..5274
                     /locus_tag="ACHAYA_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8606125.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5311..5712
                     /locus_tag="ACHAYA_002369"
     CDS             5311..5712
                     /locus_tag="ACHAYA_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606126.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5824..6177
                     /locus_tag="ACHAYA_002370"
     CDS             5824..6177
                     /locus_tag="ACHAYA_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606127.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6211..6654
                     /locus_tag="ACHAYA_002371"
     CDS             6211..6654
                     /locus_tag="ACHAYA_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8606128.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6671..7756
                     /gene="uxuA"
                     /locus_tag="ACHAYA_002372"
     CDS             6671..7756
                     /gene="uxuA"
                     /locus_tag="ACHAYA_002372"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8606129.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7749..8462
                     /locus_tag="ACHAYA_002373"
     CDS             7749..8462
                     /locus_tag="ACHAYA_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8606130.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8669..8818)
                     /locus_tag="ACHAYA_002374"
     CDS             complement(8669..8818)
                     /locus_tag="ACHAYA_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606131.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            9214..9414
                     /locus_tag="ACHAYA_002375"
     CDS             9214..9414
                     /locus_tag="ACHAYA_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8606132.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3181 a         1493 c         2102 g         2699 t
ORIGIN      
        1 tatcatggaa attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt
       61 aaaagagcac catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc
      121 aagagaaaat gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca
      181 gaaaaaaatc atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa
      241 actttttcca aaactcggac caggagacat tattattgaa ggcggaaact cttattataa
      301 agattcaatc cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg
      361 gacttctgga ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa
      421 agaaactttt ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct
      481 gtatgctggt gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata
      541 tggaatgatg caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt
      601 tgacttggaa caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat
      661 ggaaatcatg gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt
      721 ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt
      781 tccagtaatt gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc
      841 agcaaaagta gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg
      901 tgagtaaaat gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct
      961 ttccacacca cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc
     1021 aagagattcc aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa
     1081 gtgttacgcc attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt
     1141 cccgacatgg tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa
     1201 tcgtatctat catacctgct ttaattgaac gaaatgctgt agcagaaaat aatataacta
     1261 atttgttgtc catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat
     1321 gggaagatcg ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg
     1381 gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg
     1441 ttctagctta tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag
     1501 tagaacttca agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag
     1561 aagagaacta tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc
     1621 tatctaatag tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa
     1681 gcggaaagat cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc
     1741 atccgtattt agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg
     1801 aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc
     1861 ttcttcctga acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat
     1921 ggtgaacggt ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat
     1981 ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg
     2041 aacggtatca tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca
     2101 atttttttat catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac
     2161 aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat
     2221 ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct
     2281 agtaattgtg ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa
     2341 gggaaaacag aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg
     2401 attgattccc ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat
     2461 gaatctgagc tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca
     2521 caagatactg cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa
     2581 gatatggcac aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct
     2641 gttgctggtt tagttggagg attaggagat aaatacgcaa gaaatgcggc agcacatgct
     2701 atgcatgacg ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa
     2761 gtagcgtatg ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag
     2821 ttatatcctt tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt
     2881 ccgaaagata aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg
     2941 catttgattc ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa
     3001 ggttatatca gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag
     3061 tttgttgatt gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac
     3121 gttgacctta cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga
     3181 agttgcacaa gcgatgattg ttacagccgc tttgcaaatg atttatatgg gggttttttc
     3241 tccaggagga tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc
     3301 attattaggg gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct
     3361 cggaagttac ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga
     3421 caaagctgta gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac
     3481 ggttgcctca tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc
     3541 attgatcgct gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt
     3601 agtaggtgga gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa
     3661 agattatctg gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga
     3721 tattacgatg gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc
     3781 gcaaaaaggg aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga
     3841 cgaagatgac tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg
     3901 gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg
     3961 agatggcatt ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta
     4021 ctatacggaa tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca
     4081 gcatataaac ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt
     4141 actggcctaa tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag
     4201 atcttgatgc aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca
     4261 ggaatcggag atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta
     4321 ccttgggcac aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg
     4381 tatcaggttt tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct
     4441 acaggcctaa tgcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg
     4501 ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt
     4561 gcgatatccg gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata
     4621 ttacctttag ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc
     4681 acacaagcat tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta
     4741 taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt
     4801 ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt
     4861 gttgtagacg aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga
     4921 agccgtacag gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat
     4981 gaatatcaat ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac
     5041 gacttagtaa aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa
     5101 aatcctgata cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa
     5161 aaattgaaag aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca
     5221 tctgcaccta aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat
     5281 gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg
     5341 gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt
     5401 ctttcagcat tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac
     5461 gatacgctag cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa
     5521 gttatctgta cttattacga tacacccgtt catgcattag gaagttattt catgtttgat
     5581 tggaaagacc aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc
     5641 agtttaggtt ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg
     5701 ctatccgtat aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga
     5761 aaagcagctc gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta
     5821 acgatgctag aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat
     5881 gaaggctgtt atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct
     5941 attctttttt atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat
     6001 ttttcaaata aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat
     6061 tatattttta gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta
     6121 cgagggaaaa catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta
     6181 aaaagtgcaa aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt
     6241 aagttgagcg aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt
     6301 gaaacaatgt gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa
     6361 aaattaacta tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca
     6421 agtccgtaca atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc
     6481 acttatgtca tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg
     6541 cttcacacac cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta
     6601 actgtttggc atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa
     6661 ggctgaaaag atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt
     6721 aaaatatgtt cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat
     6781 gcctggagat gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca
     6841 aggactggaa ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac
     6901 agatgaacga gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg
     6961 tggggttcga ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt
     7021 gtttaatgaa aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca
     7081 gatggagcca ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc
     7141 gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat
     7201 gaccgaagaa gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg
     7261 cgaaaaagca gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg
     7321 ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc
     7381 tccatataat ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt
     7441 agttgaaatc atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga
     7501 gtttttagga gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga
     7561 tatgtatcat attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga
     7621 tcatggtaga accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc
     7681 aatgggactc acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg
     7741 agagcattat gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa
     7801 ctggacgttt agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc
     7861 gtaaggaagt ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg
     7921 gcattttgta cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata
     7981 aagaaaaaat cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt
     8041 tatcttttgc aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa
     8101 aagtagaaat gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag
     8161 gggatgatgt aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa
     8221 tgaatattcc tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag
     8281 caaggcgagc gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc
     8341 ataatgggaa gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact
     8401 atgaatttta tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct
     8461 aataaagagg actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag
     8521 atgaaaaaga agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag
     8581 aacaaacaca aaacgcatct actgttatct accagtagat gcgtttaaag agaagaacga
     8641 gaatcgtcca tatgagggtt tccactcctt atgcagattt ttttaattct tgcactgctt
     8701 gttcgatcgt aacacctgtc gctacatgat tggtattttc tttgtcaaaa acgacaaata
     8761 gattcaaatc tgtatccaag gttactcgat attgtatatc tttgttatag aatgtcatag
     8821 ccattctcct ttctttaaat acttgctgaa aggcaacgaa cgcgttgata acgaaattcc
     8881 atttataagt ataccaccaa ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag
     8941 ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact
     9001 ttttcttcga ttcgaagaca atagaagaca atgaatttta tatttcattg cattatcctt
     9061 gtgttattag aaaaagcatg atatattaaa tgagtaatct attttgaaac gtaatttgag
     9121 cgatatatct acatagataa aactcctttt gcctatcaaa tatttattgc aacgaaaaaa
     9181 cgtacattga tacgtatcag gaggaattta tttatgaata acggtacagt aaaatggttc
     9241 aacgcagaca aaggttttgg ctttatcact ggtgaagatg gaaatgatgt atttgcacat
     9301 ttttcagcta tccaaggtga cggcttcaaa actttagaag aaggccaagc agtgtcttac
     9361 gatgttgaag atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc ttaataaaaa
     9421 ttgactttta accgtctttt cgaaagaaaa gacggttttt ttgtacgaaa aaatt
//
LOCUS       DAYYCW010000101         9298 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000101 DAYYCW010000000
VERSION     DAYYCW010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.103"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..697)
                     /locus_tag="ACHAYA_002376"
     CDS             complement(29..697)
                     /locus_tag="ACHAYA_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8606133.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            831..1118
                     /locus_tag="ACHAYA_002377"
     CDS             831..1118
                     /locus_tag="ACHAYA_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606134.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1534..3072
                     /locus_tag="ACHAYA_002378"
     CDS             1534..3072
                     /locus_tag="ACHAYA_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM8606135.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3069..4034
                     /locus_tag="ACHAYA_002379"
     CDS             3069..4034
                     /locus_tag="ACHAYA_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8606136.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4021..5343
                     /locus_tag="ACHAYA_002380"
     CDS             4021..5343
                     /locus_tag="ACHAYA_002380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8606137.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5406..5510
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5584..6327
                     /locus_tag="ACHAYA_002381"
     CDS             5584..6327
                     /locus_tag="ACHAYA_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8606138.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6342..7334
                     /locus_tag="ACHAYA_002382"
     CDS             6342..7334
                     /locus_tag="ACHAYA_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8606139.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7346..8083
                     /locus_tag="ACHAYA_002383"
     CDS             7346..8083
                     /locus_tag="ACHAYA_002383"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8606140.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8073..8795
                     /locus_tag="ACHAYA_002384"
     CDS             8073..8795
                     /locus_tag="ACHAYA_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8606141.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8879..>9298)
                     /locus_tag="ACHAYA_002385"
     CDS             complement(8879..>9298)
                     /locus_tag="ACHAYA_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8606142.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3047 a         1603 c         1965 g         2683 t
ORIGIN      
        1 aattaaataa agtagtgaaa aataagaatc agtgctctaa ctgttcaaga ggatcaagtg
       61 gttcaaatgt ttctgtagtc tgttttgctt ccaattgggg caaaacagat ccaatttcca
      121 gatacttcac tttattcaat gtataagata gaatagaaca gctaagtaat gtatacagaa
      181 aatgtgtgag atccaaataa agcaaggaga caactaaaat accaaaagta atcaagaaaa
      241 gacttttgcc aatcttaata tgaaaatatt tttcaatggc ttgataaatg atatctgttc
      301 cgcctgtgct tccgccgaat cgaaagacga gaccgcaacc aatgccagaa agaatacctg
      361 tcatcaaact attgatcagt agactatcta cagcaaaatg tatatgaagg ctctcccata
      421 aatgtaaaaa gaaaactagc gcaaatgttc cgacgatcgt ccggagaaaa gcgtgttttc
      481 caaaaagaaa caatgagata agcaataaag gtaaattaaa aagcagtgaa gtgacagaag
      541 gagcgaatcc aaaaagatta tgcaaaagca aagcaatacc agtaacccca ccatctgcca
      601 gcttagatgg aatattgatt cctgtcacag ctaatccgta gattcctgcg cctaaaatga
      661 ttccgcacaa ttctgtaaat aaatttaatt ttttcatttc tagcctcttt tcttttgaaa
      721 attttcagca acttctttat tatactgact tatactctta cttcaagagg aaaatgaaaa
      781 aaaattattt ttggctgttt ctttttattt atcaaataag gagtggaaaa atgcgagcag
      841 cagacttatt gacaattctt acaaaatcac ccaaaaaaac agagtatcaa ggagtttttt
      901 tggctcaaga acaaaaactg gtgcctgtga caatggtcca gataaataaa gcaaatcaat
      961 ttatcttttt atttgaacac ggcaaagcac cacttgctat gaaagacatc tttctttttt
     1021 tgatgaagaa ccgtgagaaa gctgtttatt atcagtcaaa agaagaactc attcctcttt
     1081 atggagtgaa agagatagaa aataaagtgg tgatataagt aaaaataaga aaacaagacg
     1141 tttatgatgt caaaacaacc attttttaca tttttggcaa aaaagtgtca tgatacagta
     1201 taatattcgt tgaacaggcc aataaaggtg ttctccccca cgagatttct actttattgg
     1261 ctgtttcttt ttattaaaat aaaagttgtg tgtgtaaaaa agatatagct gattccaagt
     1321 gacaatttcg tatagatttt tatggtattc gtagtgaaaa aacgagaacc aaaaagttga
     1381 tccgactttg ttgcttggat tctttttttg tcagcatatt tagaggtatt tttttctttt
     1441 ttttgttcgt gctgtaaggt taaggcaaga caagttagtt gggaaaaagg aaagaaatgt
     1501 ttatacttaa agaataacag aaaggaaggt cgcatgaata attttataca aacattaagt
     1561 gaaagaaaag gcgagttgct ggaagctgca tttcaacatc tgtccatttc tctcatttct
     1621 ttgctgattg cagcactgat tgctattcct ttagcgattt gggcagccaa tcataaaaaa
     1681 atggcagaag tcttattgca gatcacgggt gtgctccaaa cgattccttc acttgcctta
     1741 ttaggtttac ttatcccatt tgtagggatc ggaactgttc cagcattgat tgcacttgtt
     1801 atttacgctt tgcttccgat tttccaaaac acgtatattg gtctagctga aattgacccg
     1861 tctattgaag aagcagcggt agcatttggt atgtccagaa tgagacgctt gctgaaagta
     1921 gagcttccca tcgcattacc tgtcattatc tcaggtatcc gaactgcctt agttttaatc
     1981 atcggtacgg ctactttggc agcattgatc ggagcaggtg gattagggac atttattttg
     2041 ttaggtattg accgaaatac acctgttttg acattgattg gtgcgatcag ttctgccttg
     2101 cttgcaatta tattcagcgg actgattcgc tggttgcagc ataagaaacc tcgatattcc
     2161 atcattactt tagcagcagt ggtgtgttta tttggcggcg tttctttata tcagtctgcc
     2221 ttgtttaaaa atgaaaccat cacgattgct ggaaaattag gagcggaacc tgaaatcctg
     2281 atccaaatgt ataaagaatt gattgaagac aacacaaaaa cagaagttga attaaaaccg
     2341 aattttggca agacgagttt tttgttcaat gcactggatc aaggtcaaat cgatatctat
     2401 cctgagttca caggtactgt cctagaaagc ttggtgaagg tacctgaagc aatgaaaagt
     2461 gctgatttga caaaagaaaa aacatatgac gaagcagaag aattattgaa taagcaattc
     2521 gatatgacat tattaaaacc aatggcttac caaaatacgt atgccttggc agtcaagaga
     2581 tcttttgcga acgaacatca acttaaaaag atttccgatt tggcagctat ctctgatcag
     2641 atcaaagcgg gattcacact ggaatttatt gatcgttcag atggatataa aggaatccaa
     2701 gaagcatatg gcttgaattt ttcaagtgta caaagcatgg agccaagttt gcgttaccaa
     2761 gcaatcaata atggagatgt caatattgtg gatgcctatt ccacagatag ccagctcaag
     2821 gagtacgatc tagtgactct agaagatgat cgagggttat tcccagctta tcaaggcgca
     2881 ccgctgatga aaaaagattt tgctaaagaa catcctgaaa tcgttaaagc actgaataaa
     2941 ttaagcggaa aaataacaga ggatcaaatg attgatatga attaccaagt caatgtcgaa
     3001 aagaaaaagc cgacagaagt agcacgaatc ttcctgaaaa atgaaggatt agtaaaggag
     3061 gaagatcagt gactaaatta atcgagttca aacatgtgca aaaaaagtat gatggaaagt
     3121 atgtcatcga tgacctaaat ctggcaatca aaaaaggaga aatctttgtt ttggttggtc
     3181 cttccggtag tggaaaaaca actactttga aaatgatcaa tggattatct aaaccatcag
     3241 caggagacat ctacttcaaa ggaaaatcgt taaatgaata taatctgcag aaaatgcggt
     3301 ggaatatggg atatgtgctg cagcaaatcg ctttgttccc gaccatgaca gtcaagcaaa
     3361 atattgaagt catccctgaa atgttaggtt gggaaaagca gaaaagagca gaccgtgtag
     3421 atgaacttct tcaaaaagtt ggcttatctc ctgatattta tcgagatcgt atgcctagag
     3481 agttgtctgg aggagaacag caacggatcg ggatcatccg agctattgct gcaagcccag
     3541 atgtgatttt gatggacgag ccatttagcg cattagatcc gatttcaaga aactcattac
     3601 aagaattggt tttgtcctta cacgaagaat tgggcacgac catcgttttc gttacgcaca
     3661 atatggaaga agcaatcaag ctaggtgatc gtattgcatt tatgaaggat ggagaaatta
     3721 tccaatgtga cacaccagag cagttattga tgaatcctaa aaacgactac gttcggcatt
     3781 tctttgatga accgaaacag acgaaagagt ggcgagtaga agatttagtc gtcaatggat
     3841 atttcttaaa tgaaatacca gaaaatgctc gtcaacaagt ttgctttgat acgcctatga
     3901 aagaagtata ttcgcttctt tcggtgtatc caagcattgt gatagtagaa aagcaaagaa
     3961 gtattgggtc gctgacaagt aaagaaatct ttgctttttt aagtcgagag gaggaaggaa
     4021 atgaaaacat atgatgcaat catcatcggt tcaggtgtga gcggcttaag tgctgcttat
     4081 ggattgaaag aagcaggtaa aacagtacta gtggtcgaag aagatctatg gggcgggact
     4141 tgtcctaacc gtggatgtga tcctaaaaaa gtcttgttaa gtgcagtaga ggcacgtaat
     4201 cgggtaaaac aattgagtgg aaaaggtttt aacgagatcc caactgccaa ctgggaagaa
     4261 cttcaaaaat tcaaacgaac atttacggat ccagtcccag aaagccgaaa aaaacaatta
     4321 gcagaagctg aaatcgatca tctttcagga accgctcgtt ttttggatga ttcatctatt
     4381 gaagtcaacg aagaagtctt ccatgcggat tacttagttt tggcaaccgg gcaaagacct
     4441 acgatcttac cagtggaggg aaaggaatac ttgaaaacta gtgcagactt tttatcattg
     4501 cctgtactgc ctaaagaaat catttttatc ggtggtggtt atattgcttt tgagctagct
     4561 acgattgcca atgctgcagg gagcaaagta accattgtcc atcataatca acggccatta
     4621 aaagaatttg aggcgtcttt ggtagaggaa gccgtccatc aaatggaagc atctggcatt
     4681 cagtttgctt ttggtgttga aacccaaaag atcatttcag aaggtactcg ctatcgttta
     4741 gtcggaaaag agacggaatt agtagcagat atgatttttt gtgcgactgg gagacagccg
     4801 aatacagaat ctttagcact tgagcaagcg aatatcgtct ttgataaaca cggaattgca
     4861 gtcaatgatt atctgcaaac gagcaatcca aaaatttttg cgtgtggaga tattgtttca
     4921 aggaaaaccc caaaattgac accagtcgcc acttttgaag gaaattatgt agctaagcgg
     4981 ataacggatg caacaagcga acctatcaaa tatccgatta tacctacgat cgtctacgcc
     5041 agtcctaaac ttgctgaagt aggagttaca aaaagtcacg cctcttcgtc agatcaagtg
     5101 gtagagatgg atcttaccag ttggttcacc tatcatcgtg tcaatgaacc agttgctaag
     5161 gctgaattga cctttgacca gcaaaattat ttgattggtg ctgcagtgat tagcgagcaa
     5221 gcagacgaat tgatcgatga tttgacactc gtcatcaatc aaaaattgac taaaaaagag
     5281 ttggatagct atatcatggg gtatccgaca ttggcaagtg atctctccta tttattgaaa
     5341 taatcagcat gataactaaa gcgcttgttg ctttttcctt cttgtgctac actttaaaaa
     5401 atgaatacct gtttctgggg atgtcgtaga cgacatgaga gtaagttgtg aacttagacc
     5461 cattgaacct gtcagttaat actggtgaag ggagaaacat agcagacgct tgaagcgggt
     5521 tactatgtcg tgcccatttc aagcgttttt tgttattcat attagaagaa aggaggtaac
     5581 aaaatgaaga aaaatcttta tccagcagtc agtatggcat tcattttaat tatttggcag
     5641 attgttgtca tagtttggaa aatacctgcc tttattttac ccggacctgc tgctgtattt
     5701 tctgcattag cggcagatgc acaagtatta ctttcccatt ctgtcacgac attaaatgaa
     5761 gcagtgcttg gtttggtaat agctagcgtt ctttctttta ttactgcctt ggcaatggat
     5821 tattggaagt ttttagaaat gtctttttat ccgttactag tgatttcaca aatgttgcca
     5881 atcatggtgt tagggccatt acttacccta tggtttggtt tcggtatgct tccaaaagtg
     5941 atccttgttg tcttgataag ctattttccg atcgtagttt ctttttcgga tacattgagg
     6001 aaagtatcaa aagagcaaat catctttttg aaaacgatgg gggcagatac agggaagatt
     6061 tatcgaatat ataaaatacc agcgggttta tccggcttct tttccggttt aaaagtggct
     6121 gctacttatt gtgtcagcgg agcagtcgtt ggagaatggc taagcgctca agcaggctta
     6181 gggtattaca tgatacgcgt aaaaaacagc taccagatcg ataaagtttt tgctgcgatc
     6241 atttgtgtca ttctattgag tttattgctg aatggcgctt gttcattatt gaaaaaaggg
     6301 tattatcgta tgctttatat tttttaagag aaggggatca aatgaaaaaa ttatggcttt
     6361 tattaccgct actgttgctt ttatccgctt gtggcactgc aaaagaaaca agtagcaaac
     6421 aagaaataaa ggatttgaaa aaagtgacac ttgttttgga ttatgtaccg aatactaatc
     6481 atactggcat ctacctagca aaagaaaaag gctattataa agaggctggt ttaaatgtcc
     6541 aaatcatcga accaggagat aattcgacaa gtattggtct agtaggtgcg gacaaagcac
     6601 aatttggtgt aagttatcaa gaagatgtca cttatgctca cgcagatgga cagaatatac
     6661 cagtcaaagc aattgctaca gtgatcaaac ataatacttc tggattcgcc accctatcag
     6721 acagtaacat ccattcacca aaagattttg aaggaaaaac atatgcaggt tggcaaagtc
     6781 caagtgaaga agctgtctta aaagctgtga tggaaaaaga cggcggggac ttttcaaaac
     6841 tgacaatggt aggaagcaat ggagaagggc cagaaagctt aggaaaatcc tcagatatcc
     6901 aatggtactt tgaaggttgg gatatgatca aagcaaaaga agcgggtatt gaactaaact
     6961 atatcccatt gaaagaatta gatgaacggt tagattacta tactccggta attattacga
     7021 atgatcaact gatcaaaagt gatccagaat tggtacagtc ttttatggat gccacgaaaa
     7081 aaggctatca ggaagctatt aaggatccaa atgacagtgc taaactgttg caaaaatatg
     7141 caaaagaaaa tgatcggacg tttttagaag aatcacaagc attcttgtca aaaaattata
     7201 cggatgatcc tgagaattgg ggattgatgg aagaaaaagt ttggcgcaat tatacagctt
     7261 ttatgcagga aaacggattg atcaagcaag atgtaccttc gcaagaacta ttcactaatc
     7321 agtttataaa ataggagtga tagatatgtc aatccaaatc agccacttga atgtagtcat
     7381 tgaaaacctg aaaatattag aagacatcca tttcactatc cctaaagata cctttacatc
     7441 gattgtcgcg ccaagcggag ccggtaagtc aacattgctg aaaactttaa caggagtcca
     7501 gcctatgact tctggaacga tcaaagtaga cgatcaaaaa gtaactggtc tccatacagc
     7561 atttagttat atgccgcaag aagatatgtt acttccttgg ctgagcgtct atcaaaacgt
     7621 tacgttatat caaaaaatca atcatctaac aatcaatgaa gaacaagtaa gggattatct
     7681 ggatatcttt gggttaaacg gctatgaaca ttttttgccg gaacaattat ctggcgggat
     7741 gaagcagcga acggccttgt tacgaacaat catgaatcct tcttcttact tgcttcttga
     7801 tgaaccattt ggagcgttag atgctatgac cagagggcag atgcaagact ggatgttgaa
     7861 gctgcctaaa aaagcaaagc ggactacttt acttgtgacg catgatatcg aagaagcaat
     7921 ttatttatca gacaggattc ttgtgctttc cgcccgtccg gcacatgtga ttgctgagat
     7981 tcaagtgcct gaaaaacgac ggtcacgtga atggctactg cagcaatcag agttgaagca
     8041 aacgatttat cagttattgg caggtgaatt agatgttaag tgatgctcat tgtcatttag
     8101 atgggagtca gagtttagca ctccttcagc aggaacacac gattttgacg atcatcaatt
     8161 gtgattcgcc agaagaatgg aaggaaaaca gacagcttgc agcaagtaag actcaagcct
     8221 taagttacgg tatccatcct tggaaagcag atagctatac gtttgaacaa gttgagcctt
     8281 ttttgaaaaa agcaaggatc atcggtgaaa tagggttaga taatatttgg acgaatgtac
     8341 cgatgactac acaaaaaaaa gtatttgagc gacagcttgc ttttgcagcc ataaatgaga
     8401 aacctgttgt actgcacaca aaaggatgcg aaaaagagat cttaaattat atccaagctt
     8461 accctaatca ctatttgatc cattggtatt cttctttgga ctatcaacaa gactatattg
     8521 atttaggctg ttatttcagc attggtcttg atttgaaaaa gaatccagca gtctggcaac
     8581 tagctaaagc agtcccaatc gaccacttac ttattgaaac ggatggacta catgcagcag
     8641 agtgggcttt aggggggatt ttttcagaaa aagattatgt ctcactgctt gaagaacagt
     8701 taaagacaat cgctgagatc aaagagctat cagttgaaga agttaaaaaa aatacttatc
     8761 aaaacttgga acgtttcatt agattaggta aataaaaaag agatgtgaca caactagtgt
     8821 cgcacctctt tttggctttc tttgtataaa agtaaactgt ttgattaata aatgaatctt
     8881 agtaccaacc gtttgcttcc cagaatgctt tcgccgcttc ccaagaacca taacgtgaag
     8941 taacatattg ttctgctact ctttcttggt ttgcagcaga gtagtcacca tttaagtaag
     9001 aagaatctaa ttggtaacgt ccgatgtaac gtccgttcgt tgctgtgtaa gaaccgcttg
     9061 attctttttg tgcgatccat tcttttgcag agcttgtgct tgctggagta gcagcagttt
     9121 ctgttacagc aggagcaggt tgtgtttcag caacaggcgc tgcttgtttt tccgtttgaa
     9181 ccgtttcttg tacaacaggt tgttcagctt ggactggtgc ttcttgaacg gctgcttgag
     9241 gttcagcagc tgcttgtgca gagccttctg ttgggattgt taattgttgt cctgagta
//
LOCUS       DAYYCW010000102         9093 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000102 DAYYCW010000000
VERSION     DAYYCW010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.104"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1176
                     /locus_tag="ACHAYA_002386"
     CDS             <1..1176
                     /locus_tag="ACHAYA_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8606143.1"
                     /translation="TPRACRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVTR
                     LKKQRYTCKNCRTHWTAQSYFVRLSHSIADHVRYKIASLLTEKVSLSFIAKSCQVSLT
                     TVTRTLKEFKSYLPKPSKKILPRVLMVDEFRSHASIEDKMSFICADGETGNLIAVLPT
                     RKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAL
                     NELRIREMNELRKAGQKSQAEKLKKNWRFLLKNRASINHYEYKTWKSFRAPKYPFLTE
                     AMMIDRLLEFSAPLKEAYPFFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNL
                     LTYEEGITSAMIYPYSNGKIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1355..1690)
                     /locus_tag="ACHAYA_002387"
     CDS             complement(1355..1690)
                     /locus_tag="ACHAYA_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8606144.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1674..1937)
                     /locus_tag="ACHAYA_002388"
     CDS             complement(1674..1937)
                     /locus_tag="ACHAYA_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM8606145.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2397..3200)
                     /locus_tag="ACHAYA_002389"
     CDS             complement(2397..3200)
                     /locus_tag="ACHAYA_002389"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8606146.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3178..4302)
                     /locus_tag="ACHAYA_002390"
     CDS             complement(3178..4302)
                     /locus_tag="ACHAYA_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8606147.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4318..5133)
                     /locus_tag="ACHAYA_002391"
     CDS             complement(4318..5133)
                     /locus_tag="ACHAYA_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8606148.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5123..5995)
                     /locus_tag="ACHAYA_002392"
     CDS             complement(5123..5995)
                     /locus_tag="ACHAYA_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8606149.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6080..7369)
                     /locus_tag="ACHAYA_002393"
     CDS             complement(6080..7369)
                     /locus_tag="ACHAYA_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8606150.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7513..8025)
                     /locus_tag="ACHAYA_002394"
     CDS             complement(7513..8025)
                     /locus_tag="ACHAYA_002394"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606151.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8092..8235)
                     /locus_tag="ACHAYA_002395"
     CDS             complement(8092..8235)
                     /locus_tag="ACHAYA_002395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8606152.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
     gene            complement(8718..>9093)
                     /locus_tag="ACHAYA_002396"
     CDS             complement(8718..>9093)
                     /locus_tag="ACHAYA_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8606153.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         2800 a         1942 c         1450 g         2901 t
ORIGIN      
        1 actcctcgtg cttgtagaag ttgtggttct actgtggtag atggaaacgg gaaagcaatt
       61 atagtgaaaa atgggaagaa ggaaacgatt gtccgttttg aacaatacaa tcatatgcct
      121 ttggttacgc gcctaaaaaa gcaacgctat acctgtaaaa actgtcgtac ccattggacg
      181 gctcaaagtt attttgtccg actcagccat tcaatcgcag atcatgttcg atataaaatt
      241 gcttctttac tgactgaaaa agtatctttg tcttttattg cgaaaagttg tcaggtatct
      301 ttgactaccg ttactcgtac attgaaagag tttaaaagct atttaccaaa gccttctaag
      361 aagattctcc ccagagtatt gatggttgat gaatttcgtt cgcacgcttc catagaagat
      421 aaaatgagct ttatttgcgc agatggtgaa acaggaaact taattgctgt cttgcctacg
      481 cgtaaattac ctcgattaac aagctatttc ttaggctgca ccaatccaga agaagtagaa
      541 ttcttagtca cagacatgaa cgccgcttac ttccagctta ccaaacgtgt tctgccaaat
      601 gcgaaagtag tgattgatcg gtttcatatt gtcaaacaca tgaatcaggc actcaatgag
      661 ttgcgtatcc gtgaaatgaa tgagcttcgt aaagcaggac agaaaagtca ggcagaaaaa
      721 ctgaaaaaga actggcgctt tctgctaaaa aatcgtgcaa gcatcaacca ttatgagtac
      781 aaaacatgga aaagtttccg agcaccaaaa tacccatttc tcactgaagc aatgatgatt
      841 gatcgattgc ttgagttttc tgcgcctcta aaggaggcgt atcccttttt tcatgaatta
      901 gtcgaagcct ttcgagacaa agaccctgac ttatttttct ccttattggc agaacttccc
      961 gaaacgttag atgacggctt tcgggagaag cttcaaaacc ttctgaccta tgaagaaggt
     1021 atcaccagcg caatgatcta tccttattcc aatggaaaaa ttgaagcgaa gaatacccac
     1081 ataaagacaa tgaaacgagt atcctacgga tttaaatcat tcgagaacat gagaattaga
     1141 atctttttga tcaatcaatt aatcaatgta agataacaaa aaatctgaac cagaatgtgg
     1201 ttcattctga cccggatttg attttgcaca actcatcagt ccttattgac aaagagccaa
     1261 aaatctgagc cagaatgagg ttcattctga cccagatttg attttgtaca acccatcagt
     1321 ccttattgac aaagagccct tctttttagt attattataa ttcttccatg cgttcttcca
     1381 gtcgctgtaa atcgcttgga cttaattccc caattttttt ccatttgacc tttttgttgt
     1441 ttttgggtaa ttcgtttaga gttcctacgt caatccaaga ttcttttttt agtcctgcct
     1501 gtttccaatc ccttatttca acgtatttca gctttatttt atcggattta gtatgatatt
     1561 ttgaagtaat tgacagaaac gaaattgtgt ccagtccatt ttttagaata tagacaggcc
     1621 ggcgtttacc gccctctgca gctgtgaaag caacgtagac ggttgctata tcattagtat
     1681 tcatcttcat tgaaccattc ttcaattttt tcgtcagtgt ctagtttttg gacaggcaat
     1741 ttttccgcta atctttgaat attatggatt cgtttacttt tttccgacaa ttcaatagaa
     1801 aatgggattt ttccttgctc aacaatttgc ttataaaaaa cggtgacggc tgttttgggg
     1861 ctcatgccta gttcatcaat aatgctttct gcttcatttt tcaatttttc gtccatagaa
     1921 atttgtaact tagccatttt ctccacctct ttctgacaaa cacattatat cacggaaaga
     1981 gttcaaatgg aattaataca actacttttg aactccttga aatgcagtgt atttatctaa
     2041 atataaggaa aggatatagt gctaaatggc cttgatagat agaaaagttt caaaatccgc
     2101 cacagcgata attgaagcga acactcaacg acacacagaa caggaaaaga ctcctaaaag
     2161 aaccaaatta actaggacgt gtctgaaaac tccacaaaga tgtggtatgc tctggtaaaa
     2221 aacgaatggc acaaccacga agaccgatat ggttcttgga aaactttctc cacccttttt
     2281 gtaattggag ggatagtggt ttattgatag cgattttcca agttcttcaa gtggaaccag
     2341 acattgagca ccacggctaa aaagtgaatg aagtctttat ttttaatcaa taatttttaa
     2401 tgattaccag taattggttt tttcatgcgc cctatttcta tttgatgtgg agttaaataa
     2461 tgctgataag taaaaatttt tccgtcaatt aaactgccac tagtgaatcc tgttctattg
     2521 gtaaaaagag aatagtctgg gtattcatct acaccaaatg ataaagattc agccataaac
     2581 tgttttttac cagcaaattc agcactgcta ccttgatgga tatgtcctac aaaaatacct
     2641 aaaatatcac tttcggcaat caattcagca aaaccttccg gaacttcagt tacaatagat
     2701 ggatcttccc acgacaatgg atgatgaacc aataaaatcg ttcccttttc agcaggtgta
     2761 cccaaccact cttgtaattg aatcatctgt cctatcgata tttttccttc gtgctgggta
     2821 tcttgagcag aatccaagga aataattcgt aacccatttt gattcgtaca atggatatac
     2881 tctcccgttt cattgctttc aacactcatt ccttcaaaga aaacactgcg acgatcatga
     2941 ttcccacgac aaaaatagta aggtgtggtt gggaaatatt gatccaacat tttccgtaag
     3001 acttgataat cagaaatccc cccttcatgt actaaatcac ctgttaatag aataaaatcg
     3061 tacactgtta acgagagttc ttgtaacaat ttttccaatg ccaactgtgg atttttaatt
     3121 gttggaaata gttcagcagt aaacaagtta gtttgatagt tttctctaaa atggatatca
     3181 gagagatgca aaaagcgcat ggctattccc tctttcttca ataaaataat caggatatcc
     3241 taaattttct tgcgtctgat aatcaaaaaa atgcattctt tcaggttcta tgcggagatt
     3301 gactgcatca ccaatctgcc aagtaggttt ttcattaagg acaatcattt ctataccatc
     3361 gactttaacg gtgtacgcat aactttgacc taatagctca cgatacttca cttgaccgtg
     3421 taaatcgctt gtttcttttg aaaaggttac gtgttctggt cttatgccta aatacaatga
     3481 gccactaacc tgaagaagtt gtttttgcca agctggattc aatgttaaaa attgactggc
     3541 tacctttaat gttccttgat cataagtaac tggcataata ttcatgcctg gcgaaccaat
     3601 aaaatttgca gtaaaagtat ttttaggttt cgtataaaca tttttcggcg tatcaatcat
     3661 ctgaattcta ccttcattca ataaaacaat ccggtcagct aacgtcatcg cttcaatttg
     3721 atcatgagtg acgtagacaa aagtttgttt ataacgttga tgaattttta ccaattcttt
     3781 tcgtgcatcc aatcgtaaac gcacatccaa atttgataac ggttcatcta ataaaaaata
     3841 attactccgt ttaaccaccg cacgagccaa tgctacacgc tgccgttgcc ctcctgacaa
     3901 atctttagga agtcgatctt ggtatggcgt taaacctaac atcttcaaag cttcttccaa
     3961 acggtgctga atgtcagctg tcttaatttt attcgccctt aatgcaaaaa taatattctc
     4021 ctttacagtc atgtgaggat ataaagcata attttgaaaa accattgcga tctctcgttc
     4081 tccacaaggt agcttatttg ctagttgtgt acctaaaaaa agctctccac tcgtaatttt
     4141 ttccaaacca gcaatcattc tcagcaaagt agattttcct gaaccagatg gtcccagtaa
     4201 cacaattcgt tctccagcgg ccacagctaa atcaatttct tttaaaatgg tatgttctcc
     4261 atattttttt ccgatatttc ttaattcaag cgtttctccc atgatttgtc ctccaattta
     4321 tttaatacct gcagaaacaa aagtcccgat aatatatttt tgaaaaattg catacaagac
     4381 taacggtaca aacattgtaa ttaaactaat cgccattgct acggtaaaat ttgtcccacc
     4441 ttcagaacta atataggtct gcatcgccaa cggcaaagta taattatcta ctgtccgtaa
     4501 aattaatacc ggccaaacat actcattcca agaggtaata aaaaaccaaa taccggtaga
     4561 agtaatcgca tgtctagtaa gaggaaaaac aattttactc atgacggttg tttgtttgat
     4621 gttatccaat gccgccacat ctagaaggct cactggaatt tcccgcattg tttgcgtcaa
     4681 taaaaaaatg cccatcgcat cagcaaattg tggtattatc acaccccaag tagtatcaat
     4741 taaattaagt tttgcaatca acaaataatt aggaatcatc gcaacagtaa aaggaatgaa
     4801 catcgtacca atgatgataa agtaccagat tttcttccct ttgattcggc aataagtaaa
     4861 actatatgcc gcaaaaaaag ctacgcctaa cttaacaacc gttataattg tagcaataaa
     4921 aaaagtattt ctcgtaattt tgataattgg aagattttcg aacaaatagc aaaaattttc
     4981 caaggtaaac ggctgtggta tcaaactagt aatcgtctga taggcatctt tcaatggctt
     5041 aaaagaattg gccataataa acaataatgg atagataata atgattatga tgaacataaa
     5101 taagagatgt ggcaaccagc gtttaatttt catagtaaac ccccttttcc acaaatttaa
     5161 atattacccc taagaaaaca gcaaatatta ataaagttaa aactgaaatt gctgaagatt
     5221 ctccgacttt atacatttca aaagcctttt gataagcata atagatcacg tttgcactac
     5281 cattattcgg tccaccacga gtgatgatct taatcggagt aaacacatac tgcagacctt
     5341 gaacaatggt catcaccaat aaatatagtc ctgtcgaact actcataggc aacacaattg
     5401 taaaaaaaat gcgccagatg ggtacacgat ccaacttagc tgcttcgatc atctcttgat
     5461 caattcccac aattgctgca aaaatgacaa tgaaattata accaaaaact ttccaagcag
     5521 taatacctgc aatcacagca attacccaac cttgtaaatt catccacata ggcagtctta
     5581 aaccaaaatt tcccaacaag acagcaattg gtcccgatac cggatttaat atccatgtaa
     5641 aaaccatccc cgccactacc aaagaaatca cagctggaat aaaaaataat ggtttataca
     5701 aattctgccc tttttctata actattccta ataaaaagga tgtaatatat gggaacacac
     5761 agctcagcaa gacaaataga acggtatagg acaaagtatt cttcaacact ttatgaaaga
     5821 ctggatcagt cagcaccttt acatagttgg caaagccaac aaattcttta gtgggggcca
     5881 ccatattcca gtcaaaaaaa ctcaaataga tcgtataaat caatggataa aaaacaaaca
     5941 cgcccaacaa aataattgct ggcgccagat acaaataacc tacatagcgc ttcatttccg
     6001 acagcctcct catccttatg tcaaaagcag acgaccttgt ttttcctttc ttacaggcgc
     6061 tgtcgtctgc ttgctttgct tatttgagta actcattaat ttccttttga gtagctttca
     6121 tcgtttcatt gacggaatta cctgataaaa ttttatcccg catatccaac aatttttgtt
     6181 cagcttctaa tccagcatct ccaggaaatg aactccaact gaccatctta tccatctgat
     6241 caattgctgg tatcatcatc tgattttcag ctaagaaagc ttttaatcca ttttgggcgt
     6301 ctgcaacatc tttacgtggt ggtaaataac cagtaccttc tgtccattta gctaaagatt
     6361 caacactata aagatatttt aagaaatccc aagttgcttc ctttttctta tccgtttgtg
     6421 atgtaacagc taacattgcg ccaccagcag gtagtttaac ttttttccct tcccaacttg
     6481 gtgactcaac tgctttagca gtaaaagtac tatttttttg aatgttgtcc ctttgagcaa
     6541 tagtggtata gaccatcgcg acgttaccat caataaagct ttgcacacct tgttcccacg
     6601 tagtgtgtaa tgccgtctgg tcttttacca tcctttgata taattcataa gcagcgatac
     6661 catctttaga agcaaaggta gctttgccat cttttattac acttgcccca ttactttcta
     6721 aaattgcttg ttgtgcccaa ttatcagcag gttcttgaat atacacgcca tactttccag
     6781 ttttttcttt tattatctta gaaaaagttt gaacttcttc ccaagttttt ggaccatttt
     6841 catccaagcc cgctgctttt aatatatctt gatttaaata taaaaccgga ttactaatgg
     6901 agtaaggaat acccacttgt ttaccatctt tttcagacag tgccatgata ttgtctaaaa
     6961 aatgatcttt aaaataatca gcattatccg gatccacttc ttctactaaa gattgaggat
     7021 tagtatattt gaaattttct gcaaaataat ctttaaaagc ccatcccact tgtactacgt
     7081 ccggtatttc tttggaggca acagaagctt gtaaattttg catcaagcct ttatacatat
     7141 caggattata ttttgctaca accttcactt tgtcttgaga ttgattgtat ttatctacta
     7201 attcattcac tgtttttcca cctagggtat ccgcattaac atgccaatac tctatcgtta
     7261 cacgaccctc cgattttttt gttgtcttgg tattcccact accacaagct gccaaagaga
     7321 tgacacttaa aacagacaca acccctataa caagattttt ctttaacata acattaactt
     7381 cctcctaatt tctttatggc catttttcta atcaagaaat attatgaact accaatgtaa
     7441 atttctagtt aattttctgt aaataattcg aaatttgtgc atttcggggt acagggtcac
     7501 tggacatatc catcaagcta agaataaagc ccccatatgg tcatgtatcc catctccaaa
     7561 tctcttaact cctctgtttt gattataaaa tagtttattc tgtgctcctt tcttccataa
     7621 ataggcccat gcacaacgtg aggttatatt ataaacacca cagcgcgcaa ggtactcaat
     7681 cgtttctagt tcattgactg tccatacaga aaatgtcaat cctagctgct gccccacttc
     7741 acgggccaac ttagttaacc atccttcatg aatattgacg atttgaacgc tttgataggc
     7801 taatttctgt aaggtatcga tcaaatctgc ttcggtaata tccccaagtt ttttcccttc
     7861 gaactgattt tcaatattat aaaatatatg ctttccccaa atcgcagccc aagataattg
     7921 tacatagcca ctaagaacaa tttgtggcca gatttgatag gtttgtgcca gcgaaaaaac
     7981 ggccatttcc aatccctctt cttttaaatc acagttaatg gtcatggttg ccttctcatt
     8041 ggccactgct tgaaacacct gctcaagggt taccgaatct gggacaactt cttacctgtc
     8101 atgggctagt attagcgtac catctgtttg ctgacgcaca tctacttcaa aacaaagctg
     8161 gcgatccaaa caaaagcgaa gaaatgccca gctattctcc tccgtattgt cactaccaga
     8221 gtgtgcagtg atcatggtca ttttgacccc tcctttaatt ccgtgatttt ctcccacacg
     8281 tccactatac cgatgaagcg ttaatttctt gttaatttct tgtaaattcc ctgcgttttt
     8341 tcagtaaact agttgtaaat cttgcatgct ccttcctacg aactcatgaa tcgtcgattt
     8401 tgcccttcta gctagtttca ccaattaagt agttctacat tccccgcttt taacctacta
     8461 cacctttttt tgttttcagc tattccttct tcttgctgaa agtccttgag gttctttctt
     8521 acaggatacc atcaaggtgt tcaatggctt attcccattg aatgaccgag acttactatc
     8581 agatttctgc tcgttgaacc aagtctttga tacaggattt cttccgtcat cttctcacaa
     8641 ataccacctt gcgtttctct agcgattggt cgatgtgcac cctcccagtg gactgtcact
     8701 accccttttt gctatgccta gcccacaaaa aaagctgtcc tcacagacag ccagactgta
     8761 gacaaaagct gttccaaact cgtaactcga gcttggaaca gctttttatc tacttcgttt
     8821 cgaatttttg aacaaaaaaa taaaggctcc ttgcctttcc atagccaggt ggctaatttc
     8881 tttaaattca ttgcagcaaa cactagcatc gtatgcgtcg cgactttttc caagcctcga
     8941 tacttggtcc aacgcatccc atgcttttct tttgcatccg caaatattcg ttcaatcgtt
     9001 tgtttccgtt ttttgtaaat tgatttattc agatctgtta ggcgaagatg ttccacttca
     9061 tccatcagat ctccccaaac atgtctcgtg atg
//
LOCUS       DAYYCW010000103         8986 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000103 DAYYCW010000000
VERSION     DAYYCW010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.105"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            328..924
                     /locus_tag="ACHAYA_002397"
     CDS             328..924
                     /locus_tag="ACHAYA_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFM8606154.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            905..1351
                     /locus_tag="ACHAYA_002398"
     CDS             905..1351
                     /locus_tag="ACHAYA_002398"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8606155.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            1694..2017
                     /gene="relB"
                     /locus_tag="ACHAYA_002399"
     CDS             1694..2017
                     /gene="relB"
                     /locus_tag="ACHAYA_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM8606156.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            2337..2564
                     /locus_tag="ACHAYA_002400"
     CDS             2337..2564
                     /locus_tag="ACHAYA_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM8606157.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2580..3746
                     /gene="hemW"
                     /locus_tag="ACHAYA_002401"
     CDS             2580..3746
                     /gene="hemW"
                     /locus_tag="ACHAYA_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM8606158.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3981..5024
                     /gene="hrcA"
                     /locus_tag="ACHAYA_002402"
     CDS             3981..5024
                     /gene="hrcA"
                     /locus_tag="ACHAYA_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM8606159.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            5118..5681
                     /gene="grpE"
                     /locus_tag="ACHAYA_002403"
     CDS             5118..5681
                     /gene="grpE"
                     /locus_tag="ACHAYA_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM8606160.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5732..7561
                     /gene="dnaK"
                     /locus_tag="ACHAYA_002404"
     CDS             5732..7561
                     /gene="dnaK"
                     /locus_tag="ACHAYA_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM8606161.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7712..8878
                     /gene="dnaJ"
                     /locus_tag="ACHAYA_002405"
     CDS             7712..8878
                     /gene="dnaJ"
                     /locus_tag="ACHAYA_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM8606162.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         3149 a         1451 c         1914 g         2472 t
ORIGIN      
        1 tcaaaatttc taaatcattc aatatgtagc gcttactaaa ttattcgcca taaaatttta
       61 taattggatc actcattgtt ttacctaagt catatttaag taataaccgg tgtttttaca
      121 taagaagact tcgggtattt tttagtttga ttagctctga tatacctata aagaatatgc
      181 taaggtaaga atagtaaaaa ttcacataga tgaggtataa taatgatatt tttatatatt
      241 acagataact tggcacaaaa tgagtttttt tcgatttttc aagttatcta atgcaattat
      301 attgatatca ttaggagctg cattgttatg aatccaacat tgattactaa gaaagaatta
      361 ctaaaaaaat tagatatttc tactggtgta ttagctaatt taattagaaa tggcatgcca
      421 aaagagggag aaatgtttaa tcttgataag attattacat ggcgagaaaa ttggtctaaa
      481 aacatattag gtgagttaga agttggtaga gtatatacta acaaagaaat ttcagagaag
      541 tttaaatgtt ccaaacaggg tggaatgaga agatctcatc aaactaatac tcttgtactg
      601 tttagtgatc aaacaggaag taatgtttat aaagataagt ggttaaatgg tattttacaa
      661 tataccggta tggggctaaa aggggatcaa gtattggata agaatcaaaa taaagtttta
      721 gccaattcaa aaagtaattt tgtaaagatt catttatttg aaacatttaa accaaaagaa
      781 cacacatatc tgggagaagt atatttagca ggacaaatat atactgttaa tgaaaaagat
      841 agttcgggca acagcagaaa agtatataaa tttccattag ctttaataaa tcaagagcaa
      901 ttgattgaag ataaagatat ataaccagga agaaaatcaa actagacata ttcgtaattt
      961 atctaatgct aaattagagg aagaagcaag aaaagttagt aattataata aaaaaatggc
     1021 tgcaaaatat gttaacgaag aggagttttg cttctcaaaa acaaaaatat ataaacgtaa
     1081 tcccttcgta tctgaatatg ttaaacgact tgcaaaaggt atttgtcaac tttgccaaga
     1141 aaaagggcca tttataaaag acggtgtacc ttatttacat tgtcatcata tagaatattt
     1201 gtcgcaaggt ggaaaagatg tgattgaaaa ctgtatcgcg ttatgtccca attgtcatgc
     1261 tagaattcat gaacttgagt tacctgacga taaagaaaaa ttgctgaaga tcgttgaaaa
     1321 aagagaaaat agcttttcta aaataccgta atttattttt ataggacaaa taagctaatg
     1381 tgagctatgc cttctatata agactaagtt ctattataag taacgaagta attctcgttc
     1441 cttcctgtag taatattttt taaataacct ataggcttct tcaaaagtac atcctccagt
     1501 aaattctaag tactggagga ttctttttct agtgttgctt tactcatgaa aaagtagttg
     1561 gctacttcat caaaatttct gaactttttt aatgtataat aaagagctcg atcatttact
     1621 acaaggatgc tagctcctac attttcacag ttttttagca agtaaatttt tctaaaatca
     1681 catcaggaaa tgcatgagat tctttaagag aggcaattat agggctatga ttacggttcg
     1741 tgtaagtgac gccaaaaaag aatggctttc ttatatggct gaattttatg ggattacttt
     1801 atctgaactt ctaaaaaact attcgatgga acaactagaa gatgaataca atcgtcaaac
     1861 ggctgaaatt gcccataaac attggataag aagtgggaaa acaacagtat tcatggaaga
     1921 atccttaatg aatttggtgg gctggaatga cctgccgttt aaaattcacg cccgaaattc
     1981 agaagcagct ctgattggca aatacgtgta ttggtagaga tagaagataa tcaattcgtt
     2041 gttatcgcta ttcaagtagg gcaccgaaga aatgtctatg attaagagac tgtttatttt
     2101 tacaaaaaaa taataattgg aatctagtta aaactcttga agtgagagat ttgactagat
     2161 ttttttatga ataaaataag gacaaaattg tttaggaaga atcatgagtg taaaaaaatc
     2221 atccaagaag tcgattcttg gattttttag tggatttgtt cgccaaatac gtttagtatg
     2281 cgtatatgag aggttgtaag caaagcgttg tggtctgagc acttaaggag gaatctgtga
     2341 aaatagcttt tcatattttt gcagattttg acttaatgcc gaaggtcagc tttgcgatta
     2401 ccgtttatcg agaggctgta aaagcaccgt gtagctccga tgacaataag caagcatttt
     2461 tagagctagg tttccttgat tctaaatatg cggcttattt caatgagttg gtgcttgaac
     2521 gcgattttaa gaaggtcgaa aacaggcacg acttttcgac gtaaggagaa agaatttcaa
     2581 tgatagaaac aataaatccc gcagaaaaca aggtttctgc gtacattcat atcccatttt
     2641 gcgagcatat ctgctattac tgtgacttta ataaagtttt tttagaaggg cagccagtag
     2701 atgagtatgt ggaaatgctg ctgaatgaaa tgagattaat gctggaaagt catcctacag
     2761 cgaatttgga aacactttat gtaggaggcg gaacaccaac ttctttatct gctaaacaac
     2821 ttgatcgact attatcaggt gctagagaga tcctgccttt tcaaaatggt aatgagttta
     2881 ctgtagaagc gaatcctggt gatttgaccc gtgaaaaact tcaagtgatg aaaaactatg
     2941 gcgtcaatcg tttatccatg ggagtgcaga catttgacaa tcgcttattg aaaaaaattg
     3001 gacgtaaaca tactgcagaa gatgtgtatc aaacaatgca atttttagaa gcagaaaatt
     3061 ttaccaatgt aagcatcgat ttgatttatg cattaccggg acaaacacta gaaggatatc
     3121 gggatacttt ggaaaaagct ttggctttgg atctgcctca ctattctttg tactcattga
     3181 tcctcgaaaa caaaacaatg ttcatgaact gggtacgaca aggaagactc gaactgcctg
     3241 atcaggaaac ggaaaccaga atgtttgaag agacgattga agcaatggaa aagcatggac
     3301 gtcaccaata tgaaataagt aactttgggc tctccggaca tgaatctcaa cataacctca
     3361 tgtactggaa caatgaccac tattttgggt ttggagctgg tgctagcggt tatcttggag
     3421 atattcgtta ccgcaacaaa ggaccaatcc aacactattt acgcccatta cgagcaaatg
     3481 aacgcccggt attggaagaa gaaaaattga cacgaaaaaa tcaaatagaa gaagaaatgt
     3541 tcttaggact aagaaaaaaa acagggatta ctaaaaaaca tttctatgat cgctatcaaa
     3601 aaacaattga atcggtgtac ggtgatgtgc taagggaatt agagcaagaa gggttacttg
     3661 ttaatgaaga agaccgtgtt tatttgacac caagagggac ttttttgggg aacaatgtgt
     3721 ttgagcgctt tttatttgaa aaatagtttg aagaatattt gttttttatc gaaacttgaa
     3781 aagaaaaagt ttagaaagtt tgacaatgag gtattgaagt attgatataa ctggatttac
     3841 atcaattatt tagaattagc actcttgtgt ttagagtgct aaaaaatgga gtctttctct
     3901 tgacaggaga agggcttctt gctatattta taagtgaatt agcacttaga atatatgagt
     3961 gctaatgaga ggtgaggaag atgttaacaa agcgacaaaa cgacatttta cgtctgatca
     4021 tccaacacta tacgagtagt ggcgtgcccg ttggttccaa aacattaatg tctgagggag
     4081 tcaaggccag ctcagcaacg attcgcaatg atatgaaagc attagaagat gaaggattgc
     4141 tgttgaagac tcactcttct tctgggcgga ttccatctgc tttaggctat cgttattacg
     4201 tggatcattt gttaaaacct gctcgcgtag cagaagatga tttgctgctg attcgtcagt
     4261 ctctaggcag agaatttcat gaaatcaatg agattattaa gcaatctgct gagatattat
     4321 ctgagctgac aagctatacg actttttcat tgggacctga aataaaagac cgccgactga
     4381 ctggctttcg tatcgtaccc ttgaataatc gacagattat tgctatcgtc gtaacagata
     4441 tggggaatgt agaaagccaa gtgttcactc ttcccgaata tatgggaagt caggaactag
     4501 aaaaaatggt acagattgta aacgaccgtc tagtcggaga tcctctgatg acggtgtacc
     4561 ataaacttcg tacggagatt ccgatgattc ttcataaata tttccagacg acagaaggga
     4621 tgtctcagtt atttgacacc gttcttggtc atgtttttga agaaaaggtt tatgtcagtg
     4681 gacaaatgaa tctcttggat tatgagcccc gtcaagatct tgatcagttc aaatcgatgt
     4741 tttcttttat gaaagactca gacggactta cacaaatgat cactccgatg gatagcaata
     4801 ttcatatcaa gattggtgct gaattaggca atgatttatt gcagaatatg agtatgattc
     4861 aagcaagtta cgagatagat ggtcatggaa gaggtacaat agccttgtta gggcctacta
     4921 gtatgcctta ttctcggatg ttcggtctag tcgatgttta tcgaagagaa ctagcagcta
     4981 aacttgctga ttactaccgc tcgcttgact tgtcaaactc gtaatgtgaa taacgaagct
     5041 atttgcagct gatcgttctc tctttcttag taagagcaat caaacagctg actgaataat
     5101 gaaaggaaga ctgtatcgtg aaagaaaatc aagaagaact agataaagaa atgacagatg
     5161 ctcagccaga accggaaatc gatgtcgaag ctgcagaaga ttcgggtatt tcagaagctg
     5221 aagcggaaga attcgaaaca gcgaaattaa aagctgagtt agaagaaatg gaagataaat
     5281 acttgcgggc tcgtgctgaa atcgcaaata tggcaaatcg aggaaaaaat gagcgtgagc
     5341 aattacaaaa ataccgttca caagatttag ccaaaaaatt acttccatca attgacaacc
     5401 tggaacgtgc attagccaca gaagtgtcag atgatcaagg ggcaggtttg aaaaaaggcg
     5461 tggaaatggt cttggaaagt ttgcgtaacg cactggaaga agaaggaatt gaaaaaatcc
     5521 ctgctaaagg agaagccttt gatcctaatc ttcatcaagc agtacaaact gttccagcga
     5581 ctgaagatac accagccgat acgattgtag aagtactaca agaaggctac aaattacatg
     5641 atcgcgtgtt aagaccaact atggtcattg ttgcacaata attgtgcgga tataaaactt
     5701 acactttcaa ttaaagaagg agacgttaat tatgagtaaa attatcggta ttgacttagg
     5761 tacgactaac tctgcagtag cagtattgga aggaaacgaa gcaaaaatca tcgctaaccc
     5821 agaaggaaac cgtacaacac cttctgttgt atcattcaaa aatggagaaa tccaagtagg
     5881 ggaagttgca aaacgtcaag ctgtgacaaa cccaaatacg atttcatcaa tcaaacgcca
     5941 tatcggtgaa gcaggttaca aagtagatgt agaaggtaaa tcatatacac cacaagaaat
     6001 ctcagcaatg atccttcaat atatcaaagg attcgcagaa gactatttag gtgaaaaagt
     6061 cgaaaaagca gttatcactg tgccagctta ctttaatgat gcacaacgtc aagcaacaaa
     6121 agatgctggt aagatcgctg gtctagaagt agaacgtatc gtcaatgaac caactgcagc
     6181 tgctcttgcc tatggtttag ataaaacaga tcgcgacgaa aaaatcttag tattcgacct
     6241 tggtggcggt acttttgacg tatctatcct tgaactagga gacggtgtct ttgatgtatt
     6301 gtcaacagct ggcgacaacc atctaggtgg ggacgacttt gataacaaaa tcatcgacta
     6361 catggtagca gaattcaaaa aagaaaacag cattgacttg tctcaagaca aaatggcttt
     6421 acaacgcttg aaagacgctg ctgaaaaagc gaaaaaagat ttatctggcg tatcaagcac
     6481 acaaatcagc ttgccattta tcacagcagg agaatctgga ccattgcatt tggaaatgac
     6541 attgacacgt gcaaaatttg atgaattaac agctgactta gtagaacgta caaaagtacc
     6601 agtacgtcaa gcgttaaaag atgcaggtct gtcacaatca gaaatcgacg aagttatttt
     6661 agtcggtgga tctactcgta tccctgcagt tgtagaagca gtacgtaaag aaacaggaaa
     6721 agaaccaaac aaatcagtta accctgatga agtagttgct atgggtgctg ctatccaagg
     6781 tggcgtgatc actggtgatg tgaaagatgt tgttttactt gacgttacac cattgtcatt
     6841 aggtatcgaa acaatgggtg gagtgttcac taaattgatt gaccgcaata caacgattcc
     6901 aacaagtaaa tcacaagtat tctcaactgc ggcagataat caaccagcag tagacatcca
     6961 tgtattacaa ggtgaacgcc caatggctgc tgacaacaaa actctaggaa gattccaatt
     7021 gacagatatt ccagcagctc ctcgtggtat tccacaaatc gaagtaacat tcgatattga
     7081 taaaaacggg attgtaaatg tatctgcgaa agatctaggt acacaaaaag aacaaaaaat
     7141 cacgatcaaa tcttcttcag gtctaacaga cgaagaaatc gaacgtatgg tgaaagatgc
     7201 agaagcaaac gctgaagcag ataaagctcg taaagaggaa gttgacctac gtaatgatgt
     7261 agacgcattg ctattctctg tggacaaaac attgaaagaa ttagaaggca aagtggacga
     7321 agaagaagtg aaaaaagcag aaacagctcg tgacgaatta aaagccgctg ttgaagcaaa
     7381 caacatcgag gaaatgaaaa ctaaacgcga tgcattgaac gaaatcgttc aaaacttgac
     7441 tgttaaacta tacgaacaag ctgcacaaca acaagctcaa gaaaatccag aagcagctca
     7501 aggcggagca gatgatgtcg ttgatgctga ttttgaagaa gtcgatggcg acgataaata
     7561 atcagttcac tgaatttcga gagaaggcca aagttgctac tttggctttt tcttttgaaa
     7621 aatagtttga agtatcggtt tttgtttcaa acaaaatatg atatgataac aagcgacgtg
     7681 aaaatgatga gaaccaatag gaggaaagcc tatggcaaca aaacgtgatt attatgaagt
     7741 cttagggctc tcaaaaggag catcagaaga tgaaataaag aaagcttatc gtaagctttc
     7801 gaaaaaatac catcctgaca tcaataaaga acctgatgcg gaagaaaaat tcaaagaagt
     7861 atcagaagca tatgagattt taagtgaccc gcaaaaacgc gctgcatacg accaatacgg
     7921 gcatgcaggc acagatccaa attacggtgc tggtggagct ggtggcggat ttggtggatt
     7981 cggcggcttc tctggtggcg gcttcggtgg ttttgaagat atcttcgact ctttctttgg
     8041 aggaggcggt cggtcagtcg atccaaatgc gcctcgtcaa ggtgctgatt tgcaatatac
     8101 ggtcgacttg tcttttgaag aagcgatttt tggtgtagaa aaagaaatca agtataatcg
     8161 ggaagaaatc tgtcatactt gtggtggaaa cggtgcaaaa ccaggcacac aaccaactac
     8221 ttgtcataaa tgtcatggtg caggtacgat caatgtagaa cgccaaacgc cacttggacg
     8281 agtgatgagc cgtcaaactt gtgatgtttg tcatggcaca gggaaagaaa tcaaagaacc
     8341 ttgtccaacc tgtcatggct caggacatga gaagaaagca cattcagtta aagtgaatgt
     8401 accagctggt gtagaagaag gacaacaaat gcgtttagct ggtcaaggag aagctggaga
     8461 aaacggcgga ccatttggtg atttgtatgt ggtttttcgt gtagaagaaa gtgatatctt
     8521 tgatcgtgaa ggttctgaaa tttactatga attgccttta agttttgtac aagcagcttt
     8581 gggggatgaa gtccaagtac caactgtcca tggagatgtg aaattgaaga tccctgctgg
     8641 aacacagaca ggtacgaact tccgtttacg tggaaaaggc gctccgaagt tacgtggtgg
     8701 tgcaacaggc gaccaacatg tcaaagtaaa actgatcaca ccgaaaaatc taaatgaaca
     8761 acaaagagaa gcattacgtg cattcgctga agctggcggc atcaaagttg aagaacagca
     8821 agaagacggt ttcttcgata agtttaaaga tgcttttggc gggaaaaagc ggaagtagtt
     8881 tttctagtaa aaatataaag tagacttgcc caaattttct atttctagta aaaacgctaa
     8941 gtcattaata attaaaaacc agacgaacaa tcatacgttc gcctgg
//
LOCUS       DAYYCW010000104         8979 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000104 DAYYCW010000000
VERSION     DAYYCW010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.106"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            93..392
                     /locus_tag="ACHAYA_002406"
     CDS             93..392
                     /locus_tag="ACHAYA_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606163.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(489..923)
                     /locus_tag="ACHAYA_002407"
     CDS             complement(489..923)
                     /locus_tag="ACHAYA_002407"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8606164.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1155..1865)
                     /locus_tag="ACHAYA_002408"
     CDS             complement(1155..1865)
                     /locus_tag="ACHAYA_002408"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8606165.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1855..2715)
                     /locus_tag="ACHAYA_002409"
     CDS             complement(1855..2715)
                     /locus_tag="ACHAYA_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM8606166.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2720..3358)
                     /locus_tag="ACHAYA_002410"
     CDS             complement(2720..3358)
                     /locus_tag="ACHAYA_002410"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM8606167.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3373..3672)
                     /locus_tag="ACHAYA_002411"
     CDS             complement(3373..3672)
                     /locus_tag="ACHAYA_002411"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8606168.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3715..5199)
                     /locus_tag="ACHAYA_002412"
     CDS             complement(3715..5199)
                     /locus_tag="ACHAYA_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8606169.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5219..5680)
                     /locus_tag="ACHAYA_002413"
     CDS             complement(5219..5680)
                     /locus_tag="ACHAYA_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8606170.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5698..6765)
                     /gene="ulaG"
                     /locus_tag="ACHAYA_002414"
     CDS             complement(5698..6765)
                     /gene="ulaG"
                     /locus_tag="ACHAYA_002414"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM8606171.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6983..7753
                     /locus_tag="ACHAYA_002415"
     CDS             6983..7753
                     /locus_tag="ACHAYA_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8606172.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8026..8853)
                     /locus_tag="ACHAYA_002416"
     CDS             complement(8026..8853)
                     /locus_tag="ACHAYA_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8606173.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2748 a         1873 c         1398 g         2960 t
ORIGIN      
        1 tgaatcaaat atacttgtca ttcatcatga aatatagaga cttataaaat tgtttatttt
       61 tataatgtat cagtatagaa aggaaataaa acatggatta tactgttgaa tttgacagtg
      121 aactacttaa gcaatttcca caagaagaac atttcatgta taaagaagca atttcccaca
      181 aagtgacaga gtatttggag cataattttt ttcgtataaa gccagtgcgt actcgttcgg
      241 caaaaggaat atacgagatg aaagtcaaaa ttaataaaaa aaattatcgc atagcatttg
      301 ctttaaaaaa aaatcatgtg ttcatttttt atattagttc gaatttgcaa aaaattgtat
      361 ttgataaaga ggtgagtaaa aaaataaaat agctgagcat tggaggatag attgagtgat
      421 actaagtcta tcctcttgtt tttctaaaga actaagctag aattttttct tttgtacgtt
      481 attatatgtt atttgtcatt accaaaaagc accttttttc ttctagtatc tatgacataa
      541 gaaataccac ccatttcctt tacttgaaca aatcctactg attcataaaa tttttttgtt
      601 ttctcagtat catctgtcaa taattcgatt tggactggtc cataactagt gatcttctcc
      661 aaagcttcat tcaaaagcac tttgcctatt ccttggcgct gatacctcgg atgaatgagg
      721 atatcttgaa taaatgcgat cgtcgcaaaa tcagtgatcc aacgaacgat tccgataatc
      781 ttcccttccc cattacgctt tatcagagat tcagattgtt caaatgctct agctaacctt
      841 gcagtgtctt ttgtgtaatt cgtccaacca acgctcttat ataattcaga aatctcatcg
      901 atcataaggg aatcaatatt catttttttc ttcctttctt atacaattct ccctctaata
      961 atacagttct ttgactattt aatctatcac atataactct cctgtaaaga ttctctcgat
     1021 tccttaaaat tcctctttcc ttttagaatg catacttttt gataaataaa aaggttgtga
     1081 taaattttct tcacaacctc tgtttctctc tatcaagtgt tatcttttaa ttttctaccc
     1141 ttaccctagc atctctattg cccgtagtat gcatactttc catgtttgcg aaaataatgt
     1201 ttatctagta aatattgcgg gatttcacat atattggggt tgatcagctc gttttctttg
     1261 gccatcagac aaatttcatc taagatcaaa ctgttttcta ctgctttcat aggtgaatcc
     1321 ccccatgtga acggtccatg tccatagaca agtactccag ggacttcgtt tggatctaat
     1381 tttctttcct taaacgtctc gacaatcaca ttccctgtat gaacttcgta agcttctctt
     1441 acttcttctt tcgttaattg gcgtgtacaa ggaaccttac cataaaaagc atcagcatga
     1501 gtcgtgccat aagctggcaa atcgcgtcct gcttgcgccc acatgacgga gtgcgtggaa
     1561 tgagtatgag taatagcagt tatatcttca aaagtttgat acaaaacaac atgtgtgggg
     1621 agatctgaag atggttttaa gctatcttct tctattatat ttcccgacaa atctgttacc
     1681 accatttgat ctgcttgcat gcattcatat ctgacaccgc ttggcttaat gacgatgact
     1741 cctagctcac ggtccacttc actcacattg ccccaagtca atttgactaa tccgtatcgc
     1801 ggcaatgcca gatttgcttc aaggacacgt tgcttcattg ctgcaatttt ttcttcacaa
     1861 gacatcatag ccagcctcct tcaatttaga acttaagtag tcttttgcct gctggatttc
     1921 tcttcgaaag tcttggcttt tttcactcca catttcaatc agaaatgttc catcataatc
     1981 tagacgtttc aaatgtctta ataatccaag aaaatcaaca caaccgctac caaaaggaac
     2041 atcacgaaac tggcctgaat attcttttgt tacagggtaa gtatctttta agtggatagc
     2101 agtgatataa tcgatccctt tctcaagttc tcgagccgga ttattttccg gccatgcgga
     2161 caaattgcct aggtctggat atacttgtag aaaaggtgaa tgaatctgtt ctttgatttc
     2221 taaaaatttt gaaatggagt tcataaatgg atcatccatg atttccacgg aaagaatgat
     2281 gccatattta gaagcttcct ttacgctttc ttttattcct tcgataaagt cttctctgga
     2341 attcattgat ttctcttcat aataaacatc gtatccagct atttggatta ctttgacgct
     2401 tagattatgc gccaaatgga ttgctttttt cattagttgt ttcgctgctt ttttcttttc
     2461 tggatcagca gaaccaaacg gaaatcgccg atgcgcactt aaacaaatcg aatggatacg
     2521 tacaccggag ctgaacatct cttctctcag ctcagctatc ttttcttcag accaatctaa
     2581 tcgtgctaac cgctcatccg tctcatcaat cgacatctcg ataaaatcga agtccatttc
     2641 ttttacaagg gaaaaacgtt ctttccatga aatatctttg ggtaacgctt tttcataaat
     2701 cccaatttga gccatttcct tacccccaga tacgaatgat ctcatctttg aagttttgtg
     2761 cggccttcat cggatcattt gctgctgtaa taccacgacc agtgataaag gtatacacat
     2821 cgatcccttc aaataagtgt aatgtgtgtg gatttaatcc accagttaca gaaacattaa
     2881 agcctagttc aatcagtttt ttgatcttcg ataaatcttt ttcaccccaa gtttcaccag
     2941 ataacagcgc atcacggctt tgatggtaga ttacctgccg gataccgatg ttatgccaat
     3001 ccatggcttg ttcaaaagtc caattgccgt agagttctac ttgcaattca cctatttctt
     3061 tttgtgctgc ctccatagtt ggaatcgttg cacaacaaat gactgtcatc cagtctgctc
     3121 ctgcttttgc aacattactc gccactgttc ctccagcgtc tgcacatttc gtatcagcga
     3181 ctagtttttt atctgggaac atactgcgaa tacagcgaat cgctttttgt ccttcctgta
     3241 agcaaagaat tgtgcccact tcaataatat ctactacatc accaactttc ataacatctc
     3301 ctaatgcatg ttctaagtca ttgtgatcta acgcaatttg caaattcggc ctgctcatct
     3361 tagttccttc ctttacttgt ttttttggta ccactttgtt tcagcaaatt tttcactgat
     3421 ttctttgact gacatcacat ttttgatacc gataactgta actccttttt tctctgcctg
     3481 agtaaacata ttcaaaaaat tgactggcgt aaaaactaca tcgtattggc cagctgaact
     3541 ttttccctct gaaatggcac aatgatggat tttggtaatt ggtacgttta gttctttcat
     3601 tgctttttcc acacttctca tcatcatcaa gcttgttcct gatccatttg cacacgatac
     3661 taaaattctc atcttaatcc ctccattatg agtgttgtct aatcagattt tcttttatac
     3721 tctctgcttt tctgcatatg tgtcatagtc ttctacaatc aaaaaataac catcaggatt
     3781 tttccgatat tgtaactgcg gaatagccaa taaaccaatg acaactacgg caattccagc
     3841 ataacctagg actttcatga ttactgtaaa caatggccaa gctgttgccc agtcgaacat
     3901 cccgatataa ccaccgtatg ttgacatccc aatccatgta gcgaataatg ctgaaccgcc
     3961 tacttgtatg attcctgaaa gaaacgggaa caggcaagcg gctttgaatc cacctcggtt
     4021 attggcataa actgctataa cggcattatc aaagaacaac ggaatgaaac cggctatgac
     4081 gatcgttggt gatttcaata aaatcagtaa accaatcatc aaaaactgtc ccaaagcacc
     4141 aaacaagaac ccaatcgtta ccgcatttgg cgaaccaaag ccaaaagtag cagctacgtc
     4201 aattcctgga acagctcctg gtaaaatcgt gtttgagatt ccttggaaag attgagttag
     4261 ttcatcgacg aatgtccgaa cacctaattg caagatcgcc agatataccg caaaatttaa
     4321 agaagtcgtc ataatgtaga ataagaaact ctgcccttct tgcatgaatt gcgcttgaat
     4381 caaataatct ttgcctagta caatcagaat gatcccaaag aaaaatagca tgagtaacga
     4441 agtagcaacc atattctcat tgaaaatcga caagaatcct ggtaatttga tatcttcaat
     4501 ttttttgtct tgcttgattg atctattttt cttttcatcc cgctttttca tccactcagc
     4561 taattttgca aatataaaga ttccgaacat ttgctgatgt gccactgcaa atccggcacc
     4621 ttctgtcaat tcctgcgtga ttcctactgt taagttagaa gctactgtcc aataacaacc
     4681 taaaatcagt cccataaaaa tcaatgcttg tatcctacct agatcaggaa agcaaaacag
     4741 taaaatccaa aaagcggtag ctgcttgttg aacctgaacg tttccggtag tgaaaacgga
     4801 acgcagtttc gtatatttct gaaaacggac taataagata tttaaaacaa atgcaatcag
     4861 taataaaatc atcacatctc caaaggtacg gccaaatgtc tccattaatc cagcatccac
     4921 agcattttgt ccaaaataag gatctgtaac catggcatcc aaattgaacc gttcctttag
     4981 tccaaccaag attggccgaa aattattgac caaaccacca gagccaacag tcaaaataaa
     5041 gtaaccgacc gttgccttta agaatccagc gatgcattca tgaagcgggc gtttcagcaa
     5101 aatatagcct aataacacga taaaaccaat caggtatgct ggctgcgtca agatatttgt
     5161 tgcaaaatac gtccaaatgc ttaacaaaaa ttcccccatc ttttttctcc tctatcaatt
     5221 aattatattc tgccatcaca acttggtaat catcgatgga gttcgtagag atgagacgct
     5281 caaccaatcc atctgtcatt aataattcgg ataatttttg gatattttct agatgttctt
     5341 ctggatcttt tgctgcgagt gtaaaaaaca aactagccgt tttctcctca ttctcccctt
     5401 cactttcaaa acggattggc tgtttcaact tggtaaatga aatagctgtt ccaaaaacac
     5461 catcactttt ttctgaagaa tgcggcattg ctacttgagg tgcgatcaca atataaggac
     5521 cgtatttttc tacacaatgg atgatctcct ccacatacgt ttcatcaata atttccttat
     5581 cgatcaatac aagacagctt tcggcgatag cttcacgcca atccgtgatt tctttatctg
     5641 ggtaatgaat caattgatgg tcaaaaaagt atttcagcaa gaaactcccc ttctttctta
     5701 taaaaacgac ggaaacggtg tatcattttc aatcgagaag acatcttcaa atcctcgata
     5761 aaaattaaac tcaagatcat ctttgtttgt cggatagatg aatttcccgc ctacttgcca
     5821 gatataaggt ttaaatccat acttcaggcg atcctttttg aatttccaaa tttcagtgat
     5881 ctcttttgga tcagccataa aattagacca aatatcgtag tggacaggaa tcactacttt
     5941 tgcattcaat gactccgcca tgcgcagcat atcaacagaa gtaactttgt ctgttatccc
     6001 tcgtgggttt tcaccatagg cccctaaaca gacatcgatt ttgtgttcat taccatgttt
     6061 ggcaaataaa ttggagtaat gtgaatctcc tgcatgataa agattccctc ctgatgtttc
     6121 gaataaatag tttactgcaa tttgatccat ctcctgaggc atttttcctt ttaatacctc
     6181 ttctggatct tcacaagtga ccaacgctgt ccgatcaaac gcttctaaag ctactacatt
     6241 gatgtctttg attttgactg catcacctgg ctttactgta atcgtttttt cttcaggaat
     6301 tccccatttt tgccacgtgc gaaccacttc ttttggccca atgaatgttg cttcaggaca
     6361 attttgatga actgctgcgg ctgtatggat atctagatga tcggaatgga tatgtgtaac
     6421 gattaaagcg tctacatttt ttatcgcaaa aggatcgatt acgaaaggct gagtacgtaa
     6481 attaggctgc atattttgaa cgccactcat acgcatcatc tgatggcctt ttttcatcat
     6541 gccattccca tgactacgtt ttcccgtccc acaccataga tcacataaaa tattcgttcc
     6601 ttcatgactt ttcagccaaa ttcccgtaca gcccagccac cacatagcaa aagttccagg
     6661 atccactact tctgtttcga tttcttcatt caagtatgtg ccccactcag gaaatgtttc
     6721 tactaaccac ttttcttttg ttacttcatt gacagtttta ctcatttgct tgctcccccc
     6781 atatcgttgt tccaacttac aagtaaatat taatgtgtat cagcatccat gaaaaccctt
     6841 ttatatgacc gtttgttttt tatatgattt tctttcagta atcaatgatt gtttcagtat
     6901 aataaagaca gatatgaatg tttaaatgaa caactagtca aataaatcca tggatcaaga
     6961 tgtccctcag gaggcaaaag tcatgaaaaa ctcacttgtc aatattgaaa aacgtcaaaa
     7021 agaaattttg gaactgcttc agcagactga acaactatca actttcgaat tagctgaatc
     7081 ccttgatgta tctattagta cgatacgaag agatttaaat ttattagaag aaaaaaacga
     7141 gatcatacgc aattacggtt attgttctta taattacaaa aaccagactg attttgatca
     7201 atctggtcct gaaaggatta aacaagcgat tgccagagaa gctagtaaat acattaacga
     7261 ttatgatacg ctgtttatca attctagctc gactgcgttg aaaacactgg attttcttca
     7321 atctaaccat ttgacagtca ttacaaacaa cgtgaaaatc aattactctc cgcacaaaga
     7381 taattgtagt tatattttaa ctggtggaga gatgcgcttt ccaaaagaag cactagtggg
     7441 cgacatcgca ttaaatacga tttcttcagt caatgcagac atttgtatca ttggctgtag
     7501 cggagtcgat gtagtacatg gggtcacaac aaagatatta aatgaatcaa cgatcaacga
     7561 aatgatggtc aacaagacaa tcaaatgtaa gattttagtc gctgatcatc gaaaaatcgg
     7621 actgacatca aagttcaaga ttgctgatat ttcagtcttt gattatttga tcactgataa
     7681 attttgttct gaaaaagtat tagaggagat cagaaatacg gggataaaaa ccattcaagt
     7741 agattcattt taagaaccag gacgtaaata tgtaaatagg attatcaaaa aaatgaataa
     7801 caaatttatc gataaatgaa aaacacgcta cgctcaaatt gataaaggaa tcccaagatg
     7861 tatccatcca atcgatacca tcataatcta ttgcttcttt tataaccgtt atatgttgcc
     7921 taaataggaa tcaaacaata tcgaaaaaag gaggtatgac aaaagtcaca cctccttttt
     7981 tcgaataaac ggtattcaaa tcagaacact gatttcacaa ccactttatt tcaggtcgtt
     8041 ttcaatcacc tttaccgcgt aatcatttcc gttgaaacgc gctatttttt ttgcgtccat
     8101 atatctttct aaataatcat cttctcctaa ttgcttatgg caagaagcaa tttcataaag
     8161 caagtattcc aatgcataag tcgatttatg tttcttatta tactcaatac ccttctcaca
     8221 taattggatt gctttatcat aatcctcgat tttacggtaa taggtggcga tatcataata
     8281 tacaatgcag accatcagat tatcaaaatc agtctcactc aatattttaa gtgtacgctc
     8341 cacataaact ttcgcaaaat ctaattctcc ttttgacaag tagatcattg ccacgtttgc
     8401 agctgcttgt aagtgatgga tatctgtttc ggtcgtttgt tccaacactt gattaaaata
     8461 aaataatgct gcatctggtt ttttcgcata aagatgatca agcagtcctt gataaaataa
     8521 aatttgattt aaaagatcag aattacttac tttttcctga tcgatcgttt gtaaaatttt
     8581 ataagattct tggtattttg atacagaatt caatgattga atctcattta tttttttcct
     8641 gattttgtct tcttctgttt ctactaatat atcggagatc tcgacattca gctttttgca
     8701 aagcgcactt aagatattga tgcctggcga catattcttg ttttcgatat tgctgacagt
     8761 tgcttgttca caaatgcctt ctgctaattg tacttgtgtt aacccctttt tctttcggta
     8821 ttcttttagt aatgctccat ctactttaat caaaacaatt tccttccctt taaaaaatta
     8881 tttctccttt atatcataca caaaaaaaag ttctaaacaa ccaaaaatgt ttagaacaga
     8941 ataaaaattt taattttaaa ataatattca aattaaaca
//
LOCUS       DAYYCW010000105         8940 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000105 DAYYCW010000000
VERSION     DAYYCW010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.107"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..1152)
                     /locus_tag="ACHAYA_002417"
     CDS             complement(13..1152)
                     /locus_tag="ACHAYA_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM8606174.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACHAYA_002418"
     CDS             complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACHAYA_002418"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM8606175.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACHAYA_002419"
     CDS             complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACHAYA_002419"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM8606176.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACHAYA_002420"
     CDS             complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACHAYA_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM8606177.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACHAYA_002421"
     CDS             complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACHAYA_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8606178.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6166..7257)
                     /locus_tag="ACHAYA_002422"
     CDS             complement(6166..7257)
                     /locus_tag="ACHAYA_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM8606179.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACHAYA_002423"
     CDS             complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACHAYA_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM8606180.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8800..8871
                     /locus_tag="ACHAYA_002424"
     tRNA            8800..8871
                     /locus_tag="ACHAYA_002424"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8833..8835,aa:Arg,seq:ccg)
BASE COUNT         2317 a         1897 c         1647 g         3079 t
ORIGIN      
        1 attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt cgatcatatc
       61 caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa cgacaccagg
      121 cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg catccgttgt
      181 tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa
      241 aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca attccaaatg
      301 atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa tgattttatc
      361 atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc ttgggatgct
      421 gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc ctaggacagg
      481 attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa tgaattcatt
      541 taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt cttttccgtg
      601 cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt aattggttgg
      661 ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga gctgttcccc
      721 acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc cgttaaatgg
      781 ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc cactcttcat
      841 atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa agggtgcata
      901 ggtccaagac gattcatcac cagccgccac gacatccata tgcccagata ggcctaacac
      961 ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat ctctgtaaga
     1021 aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg ctgctacttc
     1081 accttcgttt ccattcactg atttaattcg gataatctct tgtaaaatcg cgattttttc
     1141 ttcttttttc atctaatcct ctcccttgcc atttcttttt attgtatttg agtgtgctcg
     1201 aattttcaaa taggagcaac caaagtaaaa atgaagaaag agagccactt tatgaaaaat
     1261 atgctgctac ttccctccta ttttatttat acgagaggac ttgcccatag attcctattc
     1321 tactgttcta tccttaaaag ctcttatatg atgctagtta ttgttagctt tcctttttca
     1381 aggatgatct tttcttgtac cattctttgt aaacatcata tacagaagct agttgcaaaa
     1441 aaactctcta cctgctgata tttcagcaag tagagagttc gattacttaa ctaagtgatc
     1501 atactcgact atctttcaat agtcgatcat gtatcttcat tagaaaagaa ttatttgttt
     1561 tttaagttgt agaaagattt caaacctttg tattctgcaa cttcaccaag ttggtcttcg
     1621 atacgtagta attggttgta tttagcaata cggtcagtac gtgatagaga accagttttg
     1681 atttggccag cgtttgttgc aacagcgata tcagagattg ttgaatcttc tgtttcacca
     1741 gaacggtgag atacaactgc tgtgtagcca gcttctttag ccatttcgat agcttcgaaa
     1801 gtttctgtta atgtaccgat ttggttcact ttgataagga ttgagttagc aattcctttt
     1861 tcgatacctt cagataattt ttgagtgttt gttacgaaca agtcgtcacc aactaattga
     1921 actttgtcgc ctaatacgtc agttaatttc ttgaatccgt cccagtcgtt ttcgtctaat
     1981 ccatcttcga ttgagatgat tggatattta gaaactaatt cttcgtagaa tttgatcatt
     2041 tcgtcagttg ttttttcgcc ttcgcctgag tcagctagta cgtaaacacc tttttctttg
     2101 tcgtagaatt ctgaagaagc agcatccata gcaagaacaa cgtctttacc aggtacatag
     2161 ccagcttttt cgattgcttc gatgattact tcaaaacctt cttcgtttga accaaggttt
     2221 ggagcaaatc caccttcgtc accaacagaa gttgctaatc cgcgagcttt caagattgaa
     2281 gctaatgcgt ggaatacttc agcacccata cgtagagctt ctttgaatgt tggagcgcca
     2341 acaggcatga tcatgaattc ttggaagtcg attgagttat cagcatgtga tccgccgttg
     2401 atgatgttca tcattggtgt tggcaatact tttgtgttga atccgcctag gtagtggtat
     2461 aaaggtactt ctaggtagtc agcagctgca cgtgcaacag cgatagaaac acctaaaata
     2521 gcgttcgcac ctaatttacc tttgttagga gttccgtcta aagcgatcat agctttgtcg
     2581 atagccattt ggtcacgaac atcgtagcca atgattgctt cagcaatgat gttgtttacg
     2641 ttgtcgacag ctttagttac acctttaccg ccataacgag ctttgtctcc gtcacgtaat
     2701 tcaactgctt cgtattcacc agttgaagca cctgaaggaa ccattccgcg gccaaaagct
     2761 ccgctttctg tgtatacttc tacttcgatt gttgggttac cacgtgagtc taagatctcg
     2821 cgagcgtaaa catcagtaat aattgacatg ttttgtctct cctttgagtt tgttttttcc
     2881 taagggattt ccctcatata cgattttagt taattttgct cattagtgca aacatttctt
     2941 gcatttttcg caaattttca acaattattt tacagcgtct aacaatgaca agaatgaatc
     3001 tgcttgcaag cttgcgccac cgactaaagc accgtcaaca ttttcttttg ccatgtattc
     3061 agcaatattt tctggtttta cagaaccgcc gtattggata cgaactgctt ctgatacttc
     3121 ttttccataa agtttttcaa ctgtttgacg tacaactcca caaatttcgt cagcgatttg
     3181 tgcgtcagct gatttaccag taccgattgc ccagattggt tcataagcaa taaccatgtt
     3241 tgacacctgt tcagctgaaa gatcagctaa accagctgta atttgtcctt cgatccattc
     3301 agcagttttg cctgcttcat aggtttctaa agattcgccg cagcatagga ttggtgtcat
     3361 accatttgca aagatagctt tcgctttttt attgatgtct tcgtctgttt catggaaata
     3421 ttcacgacgt tctgaatgtc cgatgatcac ataatgaaca cctagatcag caagagctgc
     3481 tggtgaagtt tcaccagtga acgcacctga gttttcccag tagcagtttt gtgctgagat
     3541 ttttagttct gtaccttcag ctgatgtcat taattcttgt aagaataatg caggagatcc
     3601 gatgactgaa tcaactttgt catttgaagg gattttgttt tttactgctt cagcaaattc
     3661 ttttgcttct gatgcagttt tgttcatttt ccagttacct gcgataatag gtttacgcat
     3721 tgaggaacac actccttaat ttattgaaaa atggctaggg agtcatgaaa actcccttca
     3781 ccttttgttc gattactcgt cagaattgag caaacttggt cgtttattta tcgttgattg
     3841 ctgctagtcc tggtaattct ttaccttcaa gcaattccaa gcttgcgccg ccacctgttg
     3901 agatgtgtgt gaatttgtca gcaaatccta attgttctgc tgctgcggct gagtctccgc
     3961 caccgatgat tgttgttgcg ccttctaagt tagcgattgc ttcacaaaca ccgattgtac
     4021 ctttagcaaa gttactcatt tcaaatacac ccataggtcc gttccaaaca actgtttttg
     4081 caccttgtaa tgtatcagca aataatttta ctgatgctgg gccgatgtct aaagccatca
     4141 aaccatcagg aatgtctcct tcgattactt gtgtttcaac atcatttgaa aattctggtg
     4201 cacagacatt gtcgattggc aagacaagtt tgtcgcctgc tttttcgatt aattcttttg
     4261 ctagttccac tttatcagct tctactagag agttaccgat cttgattcct tttgcttcgt
     4321 agaatgtgta agtcattccg ccaccgatca agattttatc tgctttagga atcaagtttt
     4381 cgattacgcc gattttatca gaaacttttg cgccaccaag gattgcaatc attggtcgtt
     4441 ttggttcttc tactgcttca ccgatgaact tgatttcttt ttccatcaag aatccagcaa
     4501 ctgttgggat accagttgaa gcgataccta cgttagaagc atgtgcacgg tgagctgtac
     4561 cgaaagcatc gttaacaaat acatcgccta atgaagccca gtatttacct agttctgtat
     4621 catttttgct ttctttttta ccgtcaatat cttcaaatcg tgtattttcg aatacgacaa
     4681 cgtcgccgtc attcatgttg cggattgctt cttctaattg ttctccgcga gtttcaggta
     4741 cgaatgttac ttctttacct aataattcgc ctagacgttt tgcaactgga gctaaagatt
     4801 ttccttcttt atcttcttct gtttttacac gtccaaggtg agagaaaagg attgcttttc
     4861 cgccttgttc tagaacgtag ttgattgttg gcaatgctgc tttgatacga gtgtcgtcag
     4921 tgatcacgcc gtctttcaaa ggaacgttga agtcaacacg gacaagaact tttttgtctt
     4981 ttaaatcgat atctttcact gttttcttag ccattttatt tcctcctgaa taaacttatt
     5041 atcaaaaaaa gcggaagaag cgcgattgct tccccgcttt taaatgtaca tgtctataat
     5101 ttacacttgt acgtttgtct atcttataag ttagcgaagt attctaaagt acgtactaat
     5161 tgtgcagtgt atgacatttc gttgtcgtac caagcaacag ttttaactaa ttgtttgtcg
     5221 ccaactgtca ttactttagt ttgtgtagca tcaaataatg aaccgtaagt cataccaacg
     5281 atatctgaag aaacgatttc gtctgtgttg tatccgtaag attcgtttga agcttctttc
     5341 attgctgcat tgatttcgtc aacagttact tctttttcaa gaactgttac taattctgtt
     5401 aatgaacctg ttggtacagg aacacgttga gcagctccgt ctaatttacc gttcaattcg
     5461 ggaattacta aaccgatagc tttagcagca cctgttgagt taggaacgat gtttgcagca
     5521 gcagcgcgtg cacggcggaa gtcaccttta ggatgaggtc cgtctagagt catttggtca
     5581 cctgtgtaag cgtggatagt tgtcattaat ccttcaacaa caccaaattt gtcgttcaat
     5641 gttttagcca taggagctaa acagttagta gtacatgaag cacctgagat aactgtttct
     5701 ttaccagtta atgtttcgtg gtttgtgtta taaacgattg ttggaacatc gtttccgcca
     5761 ggagcagaga taacaacgcg tttagcacca gctgttaaat gtttttcagc tgctgattta
     5821 gaagtgaaga aaccagtaca ttctagaacg atatctacgc ctagttcgcc ccatggtaat
     5881 tcttctgggt tgcggttagc taatacttta acttcttttc cgtttacgtt gaatgaacct
     5941 tcatgaactt caactgttcc gttgaaacgt ccttgagttg tgtcgtattt taacaagtgt
     6001 gccaacattt tagcatctgt caagtcgttg attgctacta cttcgattcc atctacatct
     6061 tggatacgac ggaatgctag acgtccaata cgtccaaaac cattaatacc tactttaact
     6121 gtcatttaag atttcctcct tatgaaaatc aaaaaaatat ttattttaaa gggttacccc
     6181 ttttaaaatc tcatttgcag cagcttcatc agtgatgagc cacgtttgtt ttggtgcatt
     6241 tttcatataa gctcgaattg ctttagcttt tgattttccg cccgcgattg ccatgatgat
     6301 cgggactttt tccaaatctt gcaattctat gccgattcta gggaccttat aaacgacttc
     6361 acctttttca ttgaaaaagt agccaaaaga ttccgcaaca gcatttgctt gtttcagcat
     6421 gactaattct ttttcagtca tcttacgacg tgctgccatg tgtaatgcac gcccaatact
     6481 atgaataaca caattcgctt cactaatcaa gtttaagact tcttggatag atggctcatt
     6541 gagtaaagaa ttgtaagttg ccagacttaa ttgttccgga acataaagtg cgcggtggtt
     6601 accgtttgct ttagcagcca ttcttgcact gacagaattt gcctgtacag ttatcgcttc
     6661 accaatcccg cctcttgcag ggacaaacag gttatgccgt ttcttatttt ccagattaga
     6721 caattgttcg gctaccacag ccatagtagt tcctcccatc acagcgatga tattctcgcc
     6781 ttctggaagt agtcggtcta aggcttcgct cagcgcttcg ccgaatgcat cagccacttt
     6841 ggtctgctct tcgctgtttc ctgaaacaac gatacagcgt tggttaccaa aatattctgc
     6901 caactgttgt tcggtttgat gcgtacctaa aagctgatcc atgaagtttt caaggctttg
     6961 ataaacttct tcgcccttag ttgtcagcgt cataccgctt tttgaggtgt cgatcaaatt
     7021 cagatttttc aaaaggtctg tttctgttcg taagactcgt tctgtgatcc ccatactttc
     7081 cgataaactt cgacgcccaa tcggctgcat ccaatagata tttcgtaaaa ttttatagcg
     7141 ttcttgcaac acttcgataa tatctggtgc aacagattca atcattttta tttcatccag
     7201 catagcgatc tccttagttg gacactttac gtccaatgta gacttaaaac gacccatata
     7261 cgataaaaaa agaaattaac tattcgaatt tcacatcaat aaagggaaat ctcaacctca
     7321 cgactgtaag tctaacagtt ttgctctcgt ttttcaacta aaaagactag aaaaagtgaa
     7381 atactttttc tagtcttttg actgattagt tatcataacg ttttcttttt gatgaactcg
     7441 ggattcctaa taaatcacga tattttgcta ctgttcgtct tgagatcgct atctcatctt
     7501 ttttcagcaa ttcaaccaat ttctggtcag acaacggctt gtttttgtct tcttgatcga
     7561 ctaattcctg cagctttttc ttcgctgtat ctgctgaaag gtcttctgtc tgttctgtac
     7621 tattcgtagg aattcgtgta gtgaaaaatt tttttaattc aaatacaccg aagtctgttt
     7681 ccaaatattt cccgttgact gcacgactga ccgtggattc atgcacatct atctcagttg
     7741 caacttcctt taacgtaaga ggcttgattg gacgttcctt gtcgataaag aaagcttttt
     7801 gccgggaaac aatgacttga gcaacccgca aaattgtatc cccacgttgt aggatcgttt
     7861 tcttcaacca ttcgaattcc tgtttccgtt cttttaaata attttcagtt tctttatctg
     7921 cttgttgtga catttgtttg aagtaactct cttgaaagcg tatatttggc tgattttttc
     7981 ggtttgagat aacttgtaca tttttatcgt cgtccactaa tacccggaca tcaggaataa
     8041 tgtacaattc gctgctacgg tcgaaaatcc ggccagggct tggactcaac gtttgtaaat
     8101 agtcgaatat ctgctgaaca gcatgtagat caacagaaaa acgttgtgcg atttctttcc
     8161 attttcgctc caccagttcc tcaaaacatt cttccaacac gatataagct aattccggcg
     8221 cataatcatc tcgttctgtt tgaagcatga gacattcttg caaactacgt gcacctactc
     8281 cagctggttc taattgctga atcagagtca acgcatcgag catttggata ggcgtagctc
     8341 caatctcttt tccggcttct tctaaactaa tcgtcaaaaa tccgttcaaa tcgatgtatt
     8401 ctacaagata aagaacgatt ttcctcaaaa aagtatctcg gtaattcaaa tggacttgtt
     8461 cgatcaatga atcaaataaa gacccttttg tatcaggaat ctgatttagg taatttgtct
     8521 cttctccttc ataagaagat gaagaagctt ttatgtaatc tggctgccat tccggctcaa
     8581 caacttccac caatggattt tccattgctt gattctcaac gaatgccaac agctcctcac
     8641 tgttatattg taaaacttgg atggactgtt gcaactgttg cgtcatggct agtttctgtg
     8701 tctgctgctg tttttgtgaa aaattttgct caaatttcat ttttttgtcc aatccccttg
     8761 taatttttta tctgtttagt tacaattatc ccagtgcttg cgtccttagt gtagtggata
     8821 tcacgtaaga ttccggttct tgagacgggg gttcgattcc ctcaggacgc gttctactgc
     8881 tattttagca taatttacga atagttgctt ctttattttt aagtgaaaac tgtccgccaa
//
LOCUS       DAYYCW010000106         8757 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000106 DAYYCW010000000
VERSION     DAYYCW010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.108"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            248..1027
                     /locus_tag="ACHAYA_002425"
     CDS             248..1027
                     /locus_tag="ACHAYA_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606181.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
     gene            1073..2017
                     /locus_tag="ACHAYA_002426"
     CDS             1073..2017
                     /locus_tag="ACHAYA_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8606182.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            2033..2812
                     /locus_tag="ACHAYA_002427"
     CDS             2033..2812
                     /locus_tag="ACHAYA_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM8606183.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            2824..3522
                     /locus_tag="ACHAYA_002428"
     CDS             2824..3522
                     /locus_tag="ACHAYA_002428"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM8606184.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            3550..4314
                     /locus_tag="ACHAYA_002429"
     CDS             3550..4314
                     /locus_tag="ACHAYA_002429"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM8606185.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            4327..6144
                     /locus_tag="ACHAYA_002430"
     CDS             4327..6144
                     /locus_tag="ACHAYA_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFM8606186.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            6155..6787
                     /locus_tag="ACHAYA_002431"
     CDS             6155..6787
                     /locus_tag="ACHAYA_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8606187.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVNISGHVLLGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            6789..7907
                     /locus_tag="ACHAYA_002432"
     CDS             6789..7907
                     /locus_tag="ACHAYA_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8606188.1"
                     /translation="MENKRILLASPHMSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEE
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPV
                     FVDSDYETWNMNPEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGT
                     DVAQTLFEKGICLPSDSKMTDDDLYRVTNTIKRVMQNV"
     gene            7900..8529
                     /locus_tag="ACHAYA_002433"
     CDS             7900..8529
                     /locus_tag="ACHAYA_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8606189.1"
                     /translation="MSNRGIYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGS
                     PVIFKQQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPE
                     LWNILKGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFE
                     KDIEYVENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            8522..>8757
                     /locus_tag="ACHAYA_002434"
     CDS             8522..>8757
                     /locus_tag="ACHAYA_002434"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606190.1"
                     /translation="MSENKKKWLLIHPFDYISRTNILEEILTENSIEYFNVVSDYSHI
                     RKQFLSSKERHMIKNRHVIKTIGYKKNISVMRILS"
BASE COUNT         3105 a         1475 c         1802 g         2375 t
ORIGIN      
        1 atgaaaatta aggaaataaa aatgaaatcg attaattgtt atcttattaa tgttttattt
       61 acttttaaat cgtaaattgc cctagtcaag aaaacaattt gtggtatgat tatataggga
      121 aaacttgttt acaaatattt ctgtgaatat attaatttaa ttatgttttt taaaataaaa
      181 agctgtcatt tttatgtatg tgaattatat cttaatttat ctatcatcaa tacttaggag
      241 tgaatctatg gaaaagccat tcgtcaagtc attgactgct gtggcggtag gtgcaggtgc
      301 tattgcgatc tgtttgtttg gttaccatat caataatcag cggcaacatc agcaacggat
      361 caattatgca gaatctgcca tcaccaatca aaaagacacc ttgacttctt tatcaaaaga
      421 agtagacaag ctgtatagta caaaagaaaa aaattttcta aaccctgaaa taacagaaga
      481 aacaatcaca agtttgtcaa ataagctatc ctctgtcaaa ctttcagcag atgattacga
      541 catcaaagaa tcagaattgc ctaaagaagc tgttgcaatc caagatgaga agaaagcaat
      601 cgtgacacag ttagatagtg cagaatcaaa actgaagatc cagacagctg tcaacgattt
      661 atttactaaa aatgtatcaa actggcagca agctgtagac gatgtcgtca tcaaagaaaa
      721 ggtgacaagt gcagatgtcg ctcgtgtacg tgaaaatatg agctttttta aagatagtcc
      781 ttggaaaacg gttgttatgc aatatctagg attcgctgat actcagatcg cgcaagtcac
      841 gcaactagat cagttattcg atacgatgtt gaaggatggt caagtgactg ccaccgctac
      901 ttacgatcaa tatttgacgg cgctcagtca aatagagcag atccggaatg aaaaaataag
      961 cgcagcatac gcaacaaaag cacaaactgt tgctcagcaa atgggctata gcaatataag
     1021 ttattgacag ttaaaacgtt gattcaagca aggttagcaa acggaggaaa ctatggcaag
     1081 acgaagaaga aaatcaagag ggaaaaaaat atttctgatt attttagcag ttcttatcgt
     1141 attagtatta gcaattgtag gagtaggagc aaaattatat ctggatctct cacattctat
     1201 tgagaaaact tacgaatcag tggaacgaac ccaaggagaa cgaacgaaac cagttgatct
     1261 ggacaaacaa gaatctttct ctgttctatt gatgggcatt gatactggag atttaggtcg
     1321 ggtcgatcaa ggaagatcag atacgatgat ggttgccaca gtcagtcctg aagacaaaca
     1381 gacaacgatc gtcagcattc ctcgagatac gtatgtcgac atcgtaggtc gtgacacaaa
     1441 agacaagatc aaccacgctt acgcattcgg cggagctgcc atggcgatgg ataccctaca
     1501 aaaatatgta gacatcccaa tcgatcacta tgtctctatc aatatgaaag ggctaaagga
     1561 actcgttgat gcagttggtg gtatcgaagt caataatgac ctcactttct cacaagatgg
     1621 ctatgacttt ccaatcggta aaattacatt gaacggggat caggcattat cttattctcg
     1681 tatgcgttat gaagatccaa atggtgacta tggtagacaa gagcgtcaaa gaaagatcgt
     1741 agaaggtgta gcaaaaaaag tattgagttt aaacggtgtc agcaactatc aatccgtttt
     1801 gaatgctcta gagtcgaata tgaaaacaga catgagcttt gatgaaatga aaaaaatcgc
     1861 tttagattat cgggaggcat ttggtacgat caagcaagat cagctgcaag gagaaggctt
     1921 catgcaagat aatatctcct atcaaaaagt cagcgatcaa gaacttgatc gtgtgaaaaa
     1981 agaattaaaa gatcaaatga atctagagaa taaataggaa agtggagaaa aaatggaaga
     2041 gacaatcagc ttgaaagaaa tttttgaaac attacgcaaa cacttggtaa caatcatcat
     2101 ctcaatgttt gctggcttgg ccatctcagg aatcgtaaca tttttcatct tgacaccaaa
     2161 atacagttca caagcacaat tgatcgtgac actgccacaa tcagacacag caaatgtcaa
     2221 tgatgtcaat acgaacctgc aaatgatcaa tacgtataaa gatatgatcg tcagtgattt
     2281 agtactaaat gaagtcaaag atcgtttaga aacagaagac aatgtgaaaa tgactgctgg
     2341 acaaatcaaa gatgcgatct ccgtcaatca gtcacaaaat tcacaaatgt tctcgatcca
     2401 ggcaataagc acgaatgcag tgactgctca acagatcgct aatacaactt cacaagtttt
     2461 ccaagaaaac gccaaagacg tgatgaatgt cgataaaatc tcgattattt caggtgcagt
     2521 tgccaatacg acacctgttt cgccaaataa taaattgaat ctagtgattg gcttagtgtt
     2581 aggaatgatg gtaggcgtag gtctggcatt cttattcgaa ttacttgatc ggacagtgaa
     2641 agatgaaaaa tatgtgacgg atacattagg attcccaatc ttaggaacag ttccagaaat
     2701 gtcagcaaaa gaattgaacg ctggtatccg taaaaaagga tcagctcctg cacgaccaaa
     2761 agctacaaac gataaaacag ccccatcacg cagaagccgc tcaagagtct aggaggaaga
     2821 aaaatggcac gaacacagaa acaaaaagca actcaaactc atgcagtcag tttgatcaca
     2881 ttaacaaacc catcttcacc aatcgccgaa caatatcgta cgatccggac gaatatccag
     2941 tttgcctccg cagctggtca gcaaatcaaa acgattgtcg tgacttcatc aggtcctgga
     3001 gaaggaaaat ccaccactgc agcaaacatt gccgttgttt ttgccaaatc tggtcaacgg
     3061 gtactgctag tcgatgcaga cttacgtaag cccgtcatct ataaaacgtt caagttgaac
     3121 aacgcaagcg gattaagtac tgttcttagt tcatcaggaa gtgtagcaga tgagatccaa
     3181 cgaacaccag tcgacaattt gtcgattctg ccaagtggac ctaaaccacc aaatccatca
     3241 gaactactaa gttcgccaag aatggatcag attttagcag aagcaagaca actatttgat
     3301 gtagtgattt tcgacatgcc accagtcgtt gccgtaacag atgcacagat catgtcttca
     3361 aaaacggatg gcaccttatt ggtcgtacgg gaaaatacgt cacgaaaaga atcattgact
     3421 aaagcaaaag aactgttaga tatggtacaa gcgcgagtcc ttggtgttgt atacaatgga
     3481 gcagaacaca gcagagatgc tggctattac tacgggaatt aagaaataaa aagagaggag
     3541 acaagagaaa tgatcgatct acattgccat atcctgccag gaatcgatga cggagcagaa
     3601 aacatagatg ccagtatcgc catggctgaa aaagcgattc aacaagggat cacacatatt
     3661 ttatgcacac cccatcacaa taatggaaaa tacagcaatg aaaaatcaca agtcatttct
     3721 ctagtcgctt cgttacaagc agaattagag aaaagacaat tgcctttgac gttattggaa
     3781 ggacaagaag tacgtattac cggaacactg attgaagaca tccaccgaga tgaaatttta
     3841 tttacagatt tagatgatac atatcttcta atcgaatttc cgacactgga agtgccgtta
     3901 tatgcagaat ggctcttttt tgagttgtta caactaggaa agacaccagt gatcgtccat
     3961 ccagaacgca acgcgcattt tcgagaagac ccaaatcatt tgctgccatt cctagatatg
     4021 ggatgcttgg ctcaactcac cgcaccaagt tatgtcggtg tatttggaaa agacatccag
     4081 aaaacagcca aaatgatggt caaacataac ctcgtgcaaa tggctgcttc cgatgcccat
     4141 ggggtctcta aacgtacctt ttacttaaaa gaatgttatg agcaaatcgc caaggacttt
     4201 ggtaaggaaa aagtagcaca gatgaaacaa gtcgcaaagg acctgatcaa cggggatgag
     4261 atcagttatc cagtctatga agaggtaaaa aagaagaagt ttggtttatt ttagggggaa
     4321 tgtagggtgt ttgtattaac aagaagaaga aaaattgcca cattggtatt tgtggacagc
     4381 ttgttgttga ttgctgcgaa tattgcagca gctaaattca tgaagccgtt tgtagctata
     4441 ccaatggacc ttattttaat ctctatcggt ttgtcgattg gtttttattt attctatggt
     4501 agtttattca aagtttttac tcgaatcaat cgttatacaa atttaagaga gatcattgcg
     4561 atttttggtt cgttatctgc aagtgcggcc tcaagtatca tcatattgct tttcataaat
     4621 aaacaataca gtttacgttt agtgatcttt gcatacctac tttcgctatt actgatcatt
     4681 ggcagccgat tgatctggcg tatctatatc gaaacaaaaa atatgaggta tctatcaaca
     4741 gattccgcta aaaatacctt gatcgttggg gccggcgaag gtggacgtat tttatacaac
     4801 agctttcttg gttctaaaac ggctcaagat atccatgtca ttggattcgt ggatgatgat
     4861 ccgaataaaa gaaataccta tctttcagga aaaaaagtac tggggtcatt aaaagatata
     4921 ccggaattga tcgaaaaata tgacatccaa atggtaacga tcgctatccc ttctttatca
     4981 agaaaaaaat tacgaagaat cttcgagtta gtcgaatctg ctcatgtgaa agtcaatacg
     5041 atgccttcta tggaagaact tgcctcaggg aaaatcagcg tatcaaaatt aaaaaccatc
     5101 gacgtagtgg aattactggg aagagatgag gtagagttgg atatcgaatc catcaaagac
     5161 cagatcacaa acaaagtcat tcttgtcacc ggtgcaggag gatcaatcgg ttcagaaatc
     5221 tgccggcaaa tcatccaatt tgaccctgca aaactattac ttttaggtca tggggagaac
     5281 tcgatttact tgattgaccg tgaattacgc acgcatcatc aaaagtgtcc tacagaaatc
     5341 gtgccgatca ttgcggatat ccaagatcgc aagaaaatca atgagatcat ggaacaatac
     5401 cacccagata tcgtctacca tgcagccgct cataagcatg taccattgat ggaatataac
     5461 ccaaaagaag cagtcaaaaa caatattttc ggtacaaaaa atgtagcaga agcagccaaa
     5521 gcagcgaaag taaaaaactt cgtcatggtc tcgacagata aagccaacaa tccgccaaac
     5581 gtcatgggtt caacaaaacg aatcgctgaa atgatcgtga ctgggttgaa tgaagaagga
     5641 tgtacgaaat tctcagctgt cagatttggg aatgtccttg gttctcgcgg tagtgtgatt
     5701 ccagtattcc gggaacaaat tgctcaagga gggccagtca ctgtaacaga ctttcgtatg
     5761 acccgatatt tcatgacgat tcctgaagca agtcgtttag ttattcaatc tggggcgtta
     5821 gcaaaaggcg gcgaaatctt tattttagat atgagtgaac cagtgaaaat cgtagatcta
     5881 gcaaaaaaca tgatccgatt gagcggatat agcgaagacg agatcgaaat cattgaaaca
     5941 ggtatccgtc caggggaaaa actatatgaa gaactattgt tagataagga aagaaacgat
     6001 gaagcagttt acgagaagat ctttgtggga aacatcaaag gttattccat gcaaacagtg
     6061 atggaatttg tcaagagcct gccgcaagac gatgaacaat tggctaaaga cattgtcact
     6121 tttgcgaatg cttcgaataa gtagggggag taaagtgaaa gcaattattg taggagctgg
     6181 tggatttggc aaagagattg cttttctact agaaagtttc tctagatatg aattgattgg
     6241 ttttgtcgat gattcgttaa aaatgcaaaa tcaagaatta gtgggaaaaa ctgtattggg
     6301 aacaatagac tcgttattag aattagaaga agaaactgct atttttctgg gaatcgcttc
     6361 gccagatatc aaagagaaaa tatatcaaaa gataaataaa aataataaat tgattttccc
     6421 taatttagtt gcatcaagtg cactagtggg cataaatgtt cagttaggaa ttggcaatat
     6481 tttgatgccc tatacgactt acactgctga cgtagtttta ggaaatttca atatgatcaa
     6541 tatcggctct actatcggac atgatactca aatcggtaat tataattcaa tttttccaag
     6601 tgtaaatatt tcaggacatg ttctattagg agataaaaat gaaattggag taggtactaa
     6661 aataatacaa aatctaagaa ttggaaatat gaacattata ggtgcgggaa gtgtagtaat
     6721 aaaaaatatt aataataaga ctaaaaatgt tggcgtacct accaaagtca tagaaagatg
     6781 ggattaagtt ggaaaataaa cgaatattat tagcatctcc tcatatgagt gatgaaggat
     6841 atgaacaaga gtatgtaaaa gaagcatttg atacaaattg ggtggctcct ttgggtatga
     6901 atgtcaatgg ttttgaagag gaattagcag aatatgtcgg tgctagtcat gcagctgcac
     6961 tatcttctgg aacagcagct atacatctag ctttaaaagc ggctggggtc ggtaaagatg
     7021 atatagtgat ttgtcagtct ttgacgttct cagctactgc caatccaatc atctatcaag
     7081 gagctattcc agtatttgtt gatagtgatt acgaaacttg gaatatgaac cctgaacatt
     7141 tgcgaacagc gctagaaaaa tatcctaacg caaaagctgt attagtagtg catttgtacg
     7201 gtttatcagc tgaactagat gaaattacta aaatatgcaa agaacataat gttgttttaa
     7261 ttgaagatgc tgcagaatca ttaggaacaa catataaagg aaaatatact ggtacattcg
     7321 gcgactatgg aatttattct tttaatggaa ataagataat cacgacttca ggtggcggaa
     7381 tgcttgtgtc taatgacaaa gaaaaggtgg aaaaagtgag gttttgggct acgcaatctc
     7441 gtgagaaggc acgccattat caacacaatg aaattggcta taattatcga atgagcaata
     7501 tcgttgcagg tatcggtcga ggacaattga aagttttaaa tgaaagaatt gagaaaaaac
     7561 gatatattca tagtttttat aaaaaagaat tgtctaaatt gtcaaatata caattcatgc
     7621 cagaaaataa ttggaatgaa ccaaattatt ggttaagtgt cataacagta aagggaataa
     7681 atacgaatca tcttattgat gagttagata aaaataatat agaagcccgc ccgatatgga
     7741 aaccaatgca tttacagcca gttttcgata agtatgagta tattggtaca gatgttgctc
     7801 agacattgtt tgaaaaaggt atctgtttac cttcagattc taaaatgacc gatgatgatt
     7861 tgtatcgagt aactaataca attaagcgag tgatgcaaaa tgtctaatag aggtatctac
     7921 gaaaagttct ttaaacgttt gatagatatt cttttttcaa ttttaggttt aataatgtta
     7981 tggccactga tgacgtttgt tgcaatccta gttcgaataa agcttggatc gccagtgatc
     8041 tttaaacaac aacgacctgg aaaacatgaa aagatttttc tgatgtataa atttcgaaca
     8101 atgacagatc aaagagatga agaaggtaat ttattatcag acgatatcag attgactcaa
     8161 tttggtaaaa tattgcgttc gactagtctt gatgaactac cagaattatg gaatatttta
     8221 aaaggagata tgagtattat tggaccgcgt cctctgttag ttgagtatct ttcactatac
     8281 agtaatgaac aacgaaaaag acattttgtg cgcccaggtt taaccggatt agctcaagtc
     8341 agtggtcgta atgcaattag ttggaaagaa aaatttgaaa aagatattga atatgtggag
     8401 aacatttcaa tgatgaaaga cataaaaata ttttttttaa cgctaaagaa agttattgtc
     8461 cgtgatggaa taacatcagt agcaagtggg actactgaga aatttgaggg tgcgaagata
     8521 gatgagtgag aataaaaaaa agtggctgct tattcatcct tttgattata tcagtcgaac
     8581 gaatatatta gaggaaattc tgacggaaaa cagtatcgaa tattttaatg ttgtatctga
     8641 ttactcacat ataagaaaac aatttctatc cagtaaagaa aggcatatga ttaaaaatcg
     8701 tcatgtgata aaaacgatcg gatataaaaa aaatatatcc gtaatgcgaa ttttatc
//
LOCUS       DAYYCW010000107         8650 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000107 DAYYCW010000000
VERSION     DAYYCW010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.109"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(248..2629)
                     /locus_tag="ACHAYA_002435"
     CDS             complement(248..2629)
                     /locus_tag="ACHAYA_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8606191.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2619..3266)
                     /gene="recU"
                     /locus_tag="ACHAYA_002436"
     CDS             complement(2619..3266)
                     /gene="recU"
                     /locus_tag="ACHAYA_002436"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM8606192.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3330..3869
                     /locus_tag="ACHAYA_002437"
     CDS             3330..3869
                     /locus_tag="ACHAYA_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM8606193.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3959..4378
                     /gene="gpsB"
                     /locus_tag="ACHAYA_002438"
     CDS             3959..4378
                     /gene="gpsB"
                     /locus_tag="ACHAYA_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM8606194.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4400..4767
                     /gene="rnpB"
                     /locus_tag="ACHAYA_002439"
     ncRNA           4400..4767
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHAYA_002439"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4976..6142
                     /locus_tag="ACHAYA_002440"
     CDS             4976..6142
                     /locus_tag="ACHAYA_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM8606195.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6188..7684
                     /locus_tag="ACHAYA_002441"
     CDS             6188..7684
                     /locus_tag="ACHAYA_002441"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM8606196.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7765..8448
                     /locus_tag="ACHAYA_002442"
     CDS             7765..8448
                     /locus_tag="ACHAYA_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8606197.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8457..>8650
                     /locus_tag="ACHAYA_002443"
     CDS             8457..>8650
                     /locus_tag="ACHAYA_002443"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606198.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2720 a         1570 c         1836 g         2524 t
ORIGIN      
        1 actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta aatggataaa
       61 gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat gttagatcac
      121 acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt acatgatttt
      181 tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag acgtggtttc
      241 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
      301 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      361 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      421 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      481 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      541 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      601 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      661 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      721 tgacaggcgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      781 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      841 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      901 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
      961 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
     1021 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
     1081 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
     1141 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
     1201 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1261 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1321 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1381 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1441 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1501 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1561 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1621 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1681 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1741 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1801 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1861 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1921 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     1981 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     2041 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     2101 gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     2161 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     2221 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2281 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2341 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2401 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2461 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2521 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2581 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2641 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2701 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2761 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2821 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2881 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     2941 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     3001 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     3061 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     3121 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     3181 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3241 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3301 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3361 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3421 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3481 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3541 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactgga
     3601 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3661 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3721 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3781 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3841 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3901 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     3961 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     4021 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaaac
     4081 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     4141 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     4201 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4261 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4321 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4381 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4441 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4501 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4561 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4621 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4681 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4741 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4801 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4861 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4921 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     4981 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     5041 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     5101 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     5161 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     5221 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5281 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5341 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5401 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5461 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5521 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5581 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5641 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5701 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5761 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5821 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5881 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     5941 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     6001 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     6061 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     6121 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     6181 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6241 agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6301 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6361 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6421 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6481 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6541 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6601 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6661 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6721 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6781 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6841 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6901 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     6961 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     7021 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     7081 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     7141 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     7201 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7261 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7321 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7381 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7441 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7501 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7561 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7621 taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat atcaggtaaa
     7681 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7741 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7801 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7861 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7921 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     7981 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     8041 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     8101 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     8161 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     8221 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8281 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8341 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8401 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8461 cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac ttctggaaca
     8521 ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8581 aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8641 accgtttatt
//
LOCUS       DAYYCW010000108         8587 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000108 DAYYCW010000000
VERSION     DAYYCW010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.110"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..1512)
                     /locus_tag="ACHAYA_002444"
     CDS             complement(46..1512)
                     /locus_tag="ACHAYA_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM8606199.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1899..2495
                     /locus_tag="ACHAYA_002445"
     CDS             1899..2495
                     /locus_tag="ACHAYA_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8606200.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(2553..3035)
                     /locus_tag="ACHAYA_002446"
     CDS             complement(2553..3035)
                     /locus_tag="ACHAYA_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606201.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3141..3338)
                     /locus_tag="ACHAYA_002447"
     CDS             complement(3141..3338)
                     /locus_tag="ACHAYA_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606202.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3633..4391
                     /locus_tag="ACHAYA_002448"
     CDS             3633..4391
                     /locus_tag="ACHAYA_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8606203.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4451..4879
                     /locus_tag="ACHAYA_002449"
     CDS             4451..4879
                     /locus_tag="ACHAYA_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606204.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4925..4997
                     /locus_tag="ACHAYA_002450"
     tRNA            4925..4997
                     /locus_tag="ACHAYA_002450"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4958..4960,aa:Gln,seq:ctg)
     gene            complement(5147..5518)
                     /locus_tag="ACHAYA_002451"
     CDS             complement(5147..5518)
                     /locus_tag="ACHAYA_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8606205.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5733..5918
                     /locus_tag="ACHAYA_002452"
     CDS             5733..5918
                     /locus_tag="ACHAYA_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606206.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6001..6189)
                     /locus_tag="ACHAYA_002453"
     CDS             complement(6001..6189)
                     /locus_tag="ACHAYA_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606207.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6213..6476)
                     /locus_tag="ACHAYA_002454"
     CDS             complement(6213..6476)
                     /locus_tag="ACHAYA_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606208.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6634..6891
                     /locus_tag="ACHAYA_002455"
     CDS             6634..6891
                     /locus_tag="ACHAYA_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM8606209.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6962..7153)
                     /locus_tag="ACHAYA_002456"
     CDS             complement(6962..7153)
                     /locus_tag="ACHAYA_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606210.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7294..7614)
                     /locus_tag="ACHAYA_002457"
     CDS             complement(7294..7614)
                     /locus_tag="ACHAYA_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606211.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7637..8071)
                     /locus_tag="ACHAYA_002458"
     CDS             complement(7637..8071)
                     /locus_tag="ACHAYA_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8606212.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8406..>8587
                     /locus_tag="ACHAYA_002459"
     CDS             8406..>8587
                     /locus_tag="ACHAYA_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8606213.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2719 a         1695 c         1361 g         2812 t
ORIGIN      
        1 caggaactgc tgcaaataag caattcctgt ttttatgttc attgattaat gaatagagcc
       61 cattagtttt ttgattggtt ctttcacagt aaatagcaag ataccaccga cgattgctac
      121 tgcaccgata ataccgaagt aagcagattc tgttcccggt gtgtagaagc gtgcaatttg
      181 tgcattgatt gcttgtgaag aagcatcagc taagaaccaa atagcgattg tttgtgattc
      241 aaatgctcgt ggtgctaatt ttgttgtcac agacaaacca actggtgaaa gacacaattc
      301 agcaataacc acgatgaaga aacttcccaa caaccacaat ggacttactt ttccacttgt
      361 accataaagc atacccggca acatcaatag caagtacgat aatcctgcaa ataaaagtcc
      421 aagagagaat ttcactactg tagatggttg acgttcacca agtttcgtcc acatcgttac
      481 gaatactggt gtcagaataa agatgaaaat tggattcaat gattgatacc agcttgcttg
      541 gatcgttaca ccaaatagcg tagattgtgt acgttcacta gcaaataaag ctaagataga
      601 tgaaccttgt tcttgtaatg accagaaaat gattgctgca aggaataatg ggatataagc
      661 ccatactctt ggtttttctt cagcagttac atcttttgaa gtcagcattt tgataaagta
      721 atacaacggt aagataatcc ccaatacact gattgtattg acaaaaaagt caattgtcag
      781 atgaccagtt agttgtgccc cgccgaatac tgcagcgata acaacaagtg cgatgatcaa
      841 gttacggatg aatcctttac gttcttcagt tgtcaaagga ttcggcggtg ttgtacccac
      901 tccgtctaat gtcttgcgtc cttggatgat gtattgtaat aatccaaaga acatcccaat
      961 agcagctaaa gagaacccta aatgatagtt atattcttga ccgattgtac caacgattag
     1021 tggtgctaat aatgccccta agttgacacc catgtaaaaa atcgaaaatc ctgcatctct
     1081 tcgcaagtca gtctttgaat aaagatgtcc gaccatgcca gaaacatttg gtttcaaaaa
     1141 ggcggttccg ataataatca atgccatcga aacaaacaat gtacttacac caaatggtaa
     1201 agccaagacg atgtggccaa gcataatgaa aactccaccg aagaatacag tgcgttttgc
     1261 tcctaacact cggtcagata cccatccgcc gacgatacta gacatgtaga tcaaagatcc
     1321 gtagatcgac atgatagcta atgctgttgc tcgtggtaat ccaagtcccc cgttggcaac
     1381 tgtatcgtac atataataga taagtaatgc acgcataccg taataactaa atcgttccca
     1441 catctcagtg aagaacaaag ttgacaatcc cttcggctgc ccgaagaagc ccttgtcttg
     1501 ctgtttttcc ataatctata ccccaattct ttaaattttc agaaaatatt atacatcttt
     1561 accgaaaaat ttaactaaaa aacttattta agaaatataa actaattttc tataaaagca
     1621 agtaattttt aatggcaata tcataaaaaa aagaagatag agatttaata attatatatc
     1681 agctgttttt ttcaaataag caaaaattcc taaaacacaa caatgaaagc gataactaag
     1741 aaaatcctta cataacgatt tttcttatta aaattgtttt ttgaacttcg cttatttttt
     1801 gtgagcatat ctgtttactt actttttgta aggtgctata cttcatttcg taatcaatta
     1861 aaaaaatgca aaacgcaaat caaaggagaa attttattat gacaactttt actgacactt
     1921 tgaaaaaccg ccgttcaatc tatcatctag gacgtaatgt ttcactttct aatgaagaat
     1981 taacaacttt gatcaaagaa gcaattaaag aaagcccaac tgctttcaat gctcaatcaa
     2041 cacgcgcagt tatccttttc ggagatgcac atgaaaaact ttgggaaatc actgaagaag
     2101 cacttcgccc attgactcca gcagaagctt tcccaaatac acaaaacaaa ttagctggtt
     2161 tcaaaaatgg ttatggtaca gtattattct tcaaagatac tgacgtggta aaaggtctac
     2221 aagaacaatt tgaattatat gcagacaact tcccagattg gtcagaacaa tcaaacggaa
     2281 tcgcaacagc taatacttgg gtagcattag ttgataaagg attgggcgca aacttgcaac
     2341 attacaaccc agttattgac gaagcagttg caaaagaatg gaatatccct tcaaactgga
     2401 aactacgttc gcaattagta ttcggttcac cagaaacacc ggctgaggaa aaagaataca
     2461 tgaacgatgc cgatcgcttc cgcgtatttg gataatttta tttgaaataa aaaacagagg
     2521 ttgtgacaaa catcttgtcg caacctctgt ttttattctg gatcaatcat aatatcaatc
     2581 tctgcctcat cgtttaagcg ttgggacaaa aatggaacta attcttctaa tgatgcagaa
     2641 aatttcagca tcggtcctcg ccatccgttt cggaaaaaat tcatttttac gattttagaa
     2701 ccttttggct gatataatac aattttattg atctcagaaa aaggaacacc ttttttgtca
     2761 aagctattca aaacaagtcc ttcttctgtg attcctctac tgtccaacaa aaaactaata
     2821 aaaatcattg ctgacaagat cccttttgcc aattcatctg ctgtaggatg actgatgagt
     2881 gtataccaaa tgactagtga cactaagaag ggtatcacca agtagaccca tcgatttcgc
     2941 acagtccgat aaataatttc ttttcgaacc agccaaagaa ataaaaacat cacaacaacg
     3001 aataaaaaca atagaaccgt aaagaatgtt accatgacaa ctcgcctcgc tttttcttct
     3061 ccagaaaata ggaaaagaat actgatttac cttctcaaag aaattggact tgcagcacgc
     3121 cgcacgtcca attgttttct ttattcaaat gtatcatatt ttgcaacaac catgcttgct
     3181 ttttcatcta ataattcttg gtcctctgca gcacattggg caactttgac cacggcgcag
     3241 tttgtcattt ttgatacaac ttcaccaatg accgtttttg taaaaccaat tggtttgcat
     3301 gcatattgtt ctcctaaatt aatcatttct tccgtcatca aaatcctcct aaactgctgg
     3361 aacttttctc cacattacca ctgctatttc tttggagatt taactcattg atttttattt
     3421 acagttaaaa atcaacctta caggcattat agcgtactgt tttcgttttc acaattaaaa
     3481 cagctgttgt atttagaaga atttttttca aaaaattaaa acatattcct taaaaactaa
     3541 ttatttccta catattaaaa tataatactt ccatttccaa taaaaataca tttataatag
     3601 gaaagataaa cagctgaaaa gggagatttc aaatgaagaa aaaaacgttc aaaaaactat
     3661 tgtttctagg gggcacagct gctcttttat taagcggtac aagttattac ctacatatcc
     3721 attctccaga acatacaatg aacactgcac aaaaaagttc aacacaaaaa acgaaaaaga
     3781 caaaagagaa agcagaagtt ttactagatg ttccattaga gagccagttt gattctcctt
     3841 ctttagaaaa tggctgtgaa gtcacttcgc tttctatgct tttatctttt tatggttatg
     3901 aaacaactaa aaaccagtta gctgagcaat tggattacgt ccctgttttc aatgcagatg
     3961 ggactcatgg cgatcctaat gaaggatttg ttggtgatat cagtggcgga gactgggcta
     4021 tggtcgtcta tgttccccca gttgcctccc ttgctcagca aatcgtccaa acggattatc
     4081 acacttttcc aaaaaccgat gctcaactcg atgatatcaa aaaagcacta tctaaaggaa
     4141 atcctgtctg gacttctgtg actatcaatt ttgaagtccc agatgaaact gattttatga
     4201 cttggaccac aaataatgga gaagtaagag tcactcctct tgttcatgct tgtgtagtca
     4261 ctggatatga caaagaaaat atttatgtca acgatcccta tggtgtaaaa aatcgagcag
     4321 taccgatcaa cgatttttcg gcgattttca ctgcaatggg tggacagatg ctaactttgg
     4381 agaaaagcta gtaattcaaa caaaaatccc ttatactcaa gataccaaac ctttgaaggg
     4441 agcgtagata atggaaaaat tcacacataa aaaaatggat ccaaatgaaa ttccgattat
     4501 ttttgtccgt gactgcaagg gaaatgttca aggtaaagtc tctattaatg agtggaacga
     4561 gcgtcgcaga cctgctaccc tcaatgaact ggaaatcaaa ttgtatcgtc aatctcttgt
     4621 ttattacgca gatcaagagt acgagaaagc caccgattta ttgaaatttc taattgctcg
     4681 aacagagtat actcgctttg aatatattga acgtttagca aacatctatc atattatgaa
     4741 tgaacctgtc aaagaatatc agttattgga caccgtcttg tcagtagcag aactgattgc
     4801 ccttcctgct ggattagaaa agaaactagt acgccgcctt ttacgagtaa agcaacaact
     4861 atccgatcaa gaaaaataac ttgcgttttt ttaggaaagt gctatgatta ttttgttgaa
     4921 taaatgggat gtagccaaac tggtaaggca cttgactctg aatcaagcaa ttgtaggttc
     4981 gaaccctgcc atcccaattt gatgtgcttg ctgaaaaaat gaaccccctg taaaagaaca
     5041 atctttttgc agggggatta ttgtattatt tttactactt gtatttagtt tacataatta
     5101 aattatgaat aataaaatcg ggcgtattct taatacaaaa actgatttac ccaataattg
     5161 ctaatgcttt ctccggaact ttttctctag gaacttcctc atacgtttct atgtgtgctc
     5221 ctttattgct aattttcaaa taatgatttt cttttaacac agaaagacca ttaaattcaa
     5281 ccgttcgttc tttcccattg gcatcagcag ctttctgcac ataccttgct gtaccatatt
     5341 catttacttc ctctggtttt tgtgtcttta catagacctc tcctttttca actaatggat
     5401 ttagctgatc aagtatacca gggatttctc cataacttcc ttcagtataa tatttcagtc
     5461 caaatagcaa aataccacta atcgccgcca gtccaatcaa atatttaatc actttcatct
     5521 ttctacctcc ttgttatata ttcagtatat gggtcagtta aagaaattcc atccgtccca
     5581 agttggagat ttttctccta ctttagaagc gagaatcgag tgttttttta gtaaaaatga
     5641 tcgttatttg atattattga cgtgtggata aatcagtttt tctcgttttg tccatgtaaa
     5701 aaatcatgtt gatagcaaag gatgtgttca aaatgaatca agaagaatta tttcaaaaag
     5761 tcaaggaaat gattaagaac ggcaatttcg atggcgcaaa acgatttatc gaagaacata
     5821 aagaacaatt aggaccttat aaagagaaag cccaaaatct tttaaaagat gtaaatatcg
     5881 acagtgtcaa aaacaaattt aaaaatctat tcaaataagt aaaactttca cttttgtaaa
     5941 atcaaaggag gacaatgctg ctgcatcgtc ctcctttggt tcttttctga atacttcata
     6001 ctaaaataaa cgtgtcggat cgtaatgata agtcacattg ccgttcagtg aggcttttcc
     6061 caccacatat ttacgattct ccaacttcag ccacgcttct cgaaaagcca ttaattcttc
     6121 tctttttaca tcaatctttt cgatttttcc atcgatcaaa tcttgaataa gttgttcata
     6181 tggattcatt tttttcctcc atctgctgtc aatcaataag ttgtaaaaat aggttcatat
     6241 gcttttttgg tatctagatc ttctctgact ttgactgtcg tataaataat tttttctact
     6301 gggatactat aataacttcc ttcaccatta ttgaatcggt gaacgtcttt ctttgctaaa
     6361 ataccattta ttttattcgt cagtacacgt tgttcgacac tgttacttac aacatagtaa
     6421 atcacccgaa tattattgat caaatgcaaa tcgatttcta acactacatg atccatgaaa
     6481 atcccactcc ttctttcact ttgattatag caagtttctc ctctaatttc aaaatcgata
     6541 cagattttac cggataaatc cacaaaactg cttgcgccat ttcgcacaaa gtgttatttt
     6601 gtatcatgga ctttatttat cggaggcaat agtatgatcg cgatcggtca atttgttttt
     6661 tatattcctt tttttattat gataagcatt cttttctatt acataaaatg gacaaaaaag
     6721 aagttttcag ttttactggc ctctttacct gctgtatatt ttacttatca aattttttct
     6781 tttaggcatt gggaaacaac gtctgtatta ataacacaca tcatcgaatt gacgctttca
     6841 gtaatttttc ttattatttg gatctatttt ttatataaga atcagaattg aatccctcgc
     6901 atgtttaaaa agaaaacgat taaagatgca gcaaatttct gccgatcttt aatcgttttt
     6961 tctattctgt tataccaaaa aaatgtttga atcggtgaag gatcgttcct tgttttaatt
     7021 ctataaattc ttgtgcgatc gggtactttt cttccaaatt ttctttgttc ttttcgatca
     7081 ggcttttcgc ttcttcatat tttttatctg cacatagtgc ttttacttcc tgtaatactt
     7141 ttcctgcttc catttgcctc accctcttgc cattttattg ctttaagttt tatttaagtt
     7201 atacgcatta ttctagtttc atcccaaata accttttttc atttttactc ctcgaactaa
     7261 tcagttttct aaaggctgat tggttcgtct tttttatttt tcctccattt cattctgctc
     7321 ttctgttaat ttatctaatg ctttgattga tgctgcggct cttgcataat catagctgta
     7381 taattccata aatttatcag gcagaacaaa ccacgctgga tgataaccgt tagctgtgac
     7441 agggaatcct ttatcttgtt ggttatctcc atcccatctc agagcaacac ctttttttcc
     7501 tttccacttt actaaagcga tcgagtatcc cttctttgtt tgtgtgccgt cttcaataat
     7561 tgctaaaatt tctatttttt cttttggcgc acggacttct tgaggacgaa ccattttcta
     7621 ttcctccctt atctcattac ttcacgacca acacactgca aggggcatgt gatacgacat
     7681 aagaagcggt cgatccaact aatgcttgtt gtatagcacc ttttcctgtc gctcccataa
     7741 cgatcaaatc aatattttct tgtccaggga tgacattggc aatcaaattt cttggatctc
     7801 cagtttcaac tattgcgtgg atctcagcaa tgccgtactc tttggcatca ttgatttttt
     7861 tcagcatggc tacttctgtt ctttgttttt catctgcata gatttttgaa aaggcatagg
     7921 cactatgggt cagctcaact ttgttgatga ttgacacgat aaataagtgg gcttcattgt
     7981 cctttgctaa gtcaagggct tcagcgaacg ctttgatcga ttgtcttgat ccgtcaactg
     8041 ctaccatgat gttctggtat cttttactca ttggaaccgc ctcctttttc ttttattgta
     8101 acatctcaag caaaaataca aaaaatatac gcaatatgta aataaaatga tgaacaaaag
     8161 caatttcttt caagttattt atacatttct tttctcatca gaacacctgt tctaatccca
     8221 ttatttctgc attatgtgaa attcctcatc atttttcaca tcgagcgaac atcattctgt
     8281 tacaaaaaac gtggatttgt cataaattgt catgcttctt ttacctatga aatattttca
     8341 cgtgttaaag tattcctcgt tgaacacaac aaataaaact ttttaacgaa taaaggagat
     8401 ttttcatgac atcacttaaa actttacttt ttggaacaac tttggctgcc ggcgctgcat
     8461 tcttcatggg aacgactgct catgcggacg aagcttatac cgttcaatca ggtgatactc
     8521 tatcaacgat ttctcaaaaa tacgttggtg ataactcctt gatcaatgcc attgcagaat
     8581 caaattc
//
LOCUS       DAYYCW010000109         8537 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000109 DAYYCW010000000
VERSION     DAYYCW010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.111"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(197..421)
                     /locus_tag="ACHAYA_002460"
     CDS             complement(197..421)
                     /locus_tag="ACHAYA_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606214.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(441..863)
                     /locus_tag="ACHAYA_002461"
     CDS             complement(441..863)
                     /locus_tag="ACHAYA_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8606215.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(1005..1715)
                     /locus_tag="ACHAYA_002462"
     CDS             complement(1005..1715)
                     /locus_tag="ACHAYA_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM8606216.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(1733..2230)
                     /locus_tag="ACHAYA_002463"
     CDS             complement(1733..2230)
                     /locus_tag="ACHAYA_002463"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8606217.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(2267..2719)
                     /locus_tag="ACHAYA_002464"
     CDS             complement(2267..2719)
                     /locus_tag="ACHAYA_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8606218.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(2854..3459)
                     /locus_tag="ACHAYA_002465"
     CDS             complement(2854..3459)
                     /locus_tag="ACHAYA_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8606219.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(3909..4529)
                     /locus_tag="ACHAYA_002466"
     CDS             complement(3909..4529)
                     /locus_tag="ACHAYA_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8606220.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(4531..5400)
                     /locus_tag="ACHAYA_002467"
     CDS             complement(4531..5400)
                     /locus_tag="ACHAYA_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8606221.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(5423..5830)
                     /locus_tag="ACHAYA_002468"
     CDS             complement(5423..5830)
                     /locus_tag="ACHAYA_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8606222.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(5849..7138)
                     /locus_tag="ACHAYA_002469"
     CDS             complement(5849..7138)
                     /locus_tag="ACHAYA_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8606223.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            7196..8535
                     /locus_tag="ACHAYA_002470"
     CDS             join(7196..7637,7637..8535)
                     /locus_tag="ACHAYA_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM8606224.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT         2536 a         1688 c         1427 g         2886 t
ORIGIN      
        1 tcgatttatt aataaaacaa cgtgttttat catatactaa aacaaaacat aagtaaacaa
       61 aaaattcgga ttttaagcaa aatccgaacg ttccatctga ttaaatccct ttatctcaaa
      121 gttcatgaac attcactttg gattacaaga atttctttat tgtgttaata aagtttcttc
      181 attctagtct caaaaatcaa tagtgtgatt tgattttacc atttcgtttc tgttcttctt
      241 tgatgaattc ttttgtatcg acaggattca gattatttag tccgccaaaa cctgtatcga
      301 ccatttttcc ccagcctttt gtcttctttt ttcctgattt gagattcttt gctcgaagct
      361 tctcactatg ttttgctcgc cgatacttca aataaccttc taatcttccc attagatcca
      421 ctgtttttcc tcctttttat tcaatcagac gactcagctt ttcctataac aaaataattg
      481 ccttcagggt cagaaaaatt gaaagtcaag atgccattgt tatctgtgat ttctcctggt
      541 tcttccatgt tctcgtagat agtttcaaat tcatccgtaa aaaatactaa agaaggcggt
      601 ggtcctaata cctctggtga aaacttctgc acaaatactt ttgggaatac gcctaattct
      661 aattcttttg agatgctgag tacaatactc ttaaattctt ctggcaattc gattgtttca
      721 gaaatttctg caccaatttt ttctacaaag aatcggcaaa tcatctctac gtcattgaca
      781 taaagcatca cacgtgtttt ttgtatcatc atttgaccct tctttctatt tgattttctt
      841 tcctgtctat aaactgattc cattatatca cgaaagcgca aacaccttgt gtttgatact
      901 attcaagaaa aataaagcaa tagtttctag gattgctttc ctccacaaac tttttttaca
      961 aaaaaaaaac ggcaatgaat gccgctcttc ttatttcgat tcttttataa ttctgcttct
     1021 gattcatctt tggttagtgt gtgtctaaca ctagttacgc caaatgtttg atcgattttt
     1081 tccttcaacg cttcgattgc ctgtgcgcca cgtgccgctt gttcagcatt gatggattca
     1141 atgaccagca ccgctgcttg tgtttcttcc gtcagcatcg ttctttgggc ttcacgccag
     1201 ttgagacagc gacagatcgc tccttgttca tcatagtaga tgatttcttc cggaagtgcc
     1261 ggagcatcgg aatctgcacc taaaggcagg aatgattcgc caccttgggc ttttcctaaa
     1321 tgcagatttc ctgcgatctt ttcgatatct tctccgccgc aaggtacgcc aaaagacaag
     1381 gaaatactat tatagatatc tacaagcgga ttgattggga aaaattcccg ttcttgattt
     1441 gcacgtttca gtaaagcttc aatagaagaa cgtgcacctt ttttagtctt gaatgtggta
     1501 aatgcctgac gccactgagc aatgacttcg ttttggctga atgtttcttc tgtaaggtaa
     1561 tttcttgctt cttttgctgc ctcattcaat aattgatgat acgtttcttc actttctttt
     1621 gttacgtggt tatcgatacc atttacggat aagatttcga tggttgcttc aggaaatact
     1681 tcccagaaat tggtatccac tattactttt ttcatctgtt cacacctctt gattagaata
     1741 attgcttgat gaatagatag tctgtttggg gatcatctcc catataaaaa acatgttcgc
     1801 tgtatttgat aaatcctttt ttacgataaa aagcgagcgc aacatcgttg tgttcccaaa
     1861 caccgagcca aatggctgtt ttttgctttt cttttgcttt ttcaatagct gtatctagta
     1921 aaaatgtacc gataccttga tgtttaaatt tttttcgtac atagattcgc tcgatttcta
     1981 aaaaatgatc agccatttcc tcagattgac tcgttcctgt atttagtttc aggtaacccg
     2041 caggctcttg atcgatactc actaggaaaa aatcggtttc tttatctttt agctcttctg
     2101 taagtttttc tagtgcataa gcttgtgtca agtaatcagc taaatgctcc ggtgaattat
     2161 cttttccaaa tgtatctgta aatgtttcga tactaatttg ttgtagtaag tctacatctg
     2221 aaggcgtcat ttttcgaatt tcgtatttat tcacggcttc ttcctttcaa tagatccttt
     2281 tattgccttt tttgacaaaa ttccaatctt cgctaacatt cttgcatact ttatttaaaa
     2341 gatattccaa ttgttttgct tcttcgtcag aaagaccatt taatgcgact gcattggagt
     2401 agttgttttc tcgaatgatg aaaggataga tttctgctcc ttttttagtc ggatagagtt
     2461 tcttgatttt tttattttct ttatcttcca aacgttcgat catcccattg gattctaatt
     2521 tcttgattgc tcgggcagtc gttgtacgat ctactttgat catttctgct agtttttctt
     2581 ggatgattcc aggattttcg caaatacgta ctaaataaag atactgtcct cttgttaaat
     2641 cgacttcttt gaattcgata ttgctaatag aatctagcgc tcgggcaatc attccgatgt
     2701 ctcgaaggat ttcagccatt tcatcccacc tttattttgt ttgtaagact agttcacctc
     2761 tttttgttgc aaatgcaact attttcaata aaaaaatcaa gttctttttt aataactagt
     2821 aaagtatatt cttttcttct tttacgaaaa agctcaatca caatagatct ggtttttgtt
     2881 ttctaaaata agtaactgtt tcttcgttgt tcgcttttct gatagctttg gcaactcttc
     2941 tatactaaaa aatcccatgt tggaagtttc attattctca ataaacttgg cctcttcacc
     3001 gtggatcgca caagcaaaaa caagtttata atactgaaaa agctgtggaa tatctttttg
     3061 tttattcgta tcaaaaactg ctcgcaactg agtagtttct actgttaatc ctgtttcttc
     3121 atagacttct ttacgcacat tttcttcagg agacaatcct atttcagcaa atccaccagg
     3181 aagtgaccat tctttcgtcc gtttatcttc gattagtagg accttgccat cttttttgat
     3241 ataggcacga acatctactt ttggtgttgg atgtccttct ccaatcgttg tgagcgcttt
     3301 tatttcttcg aaagtttctt ttcctatttc actgatcagt tccaatgaaa tcgtccggat
     3361 ctcctcaaaa cgctctttat caaatacatc ttttccataa tatagaccag cttgagaaag
     3421 tgcgagtagt cttttgtatt gagccaactt cttgttcatt ctcttccctt cttttttagc
     3481 aaatgtatga ctatgtaatc aagtatagaa gactggttta attacggcaa ataaatataa
     3541 tgaaatctgc aataaataaa agaatgttaa aaaactcgag aacaaaagac attcgacttc
     3601 tctggggctg tgaataaatg gcgttcaaaa gcttgcatct gcggtaataa gccccaccaa
     3661 acgaaaaata tgacgagcta ttcttgtctg gctgttctta ttaattgagg caggacagct
     3721 ttttcacaac ctccccacta aacggtgttc tcctaccaac tcctcggtca caagtcacaa
     3781 tattcatcaa acatgagaag ctgttttttt gaaattgctc cttgtgactc ggagctgaac
     3841 gacaggctca cgacctctcg gtaaacagcg ttcaaaagcc aatttttcga tattaagcca
     3901 tttctttgtt acttattgtc attgatttct ttttctaatt tatccaatag tggagtgtaa
     3961 tccacttgag tatagtctgt tacatcaaat tcatatagtg ttcctaactg gaatttgtca
     4021 tattttcggt cggcgatgat ctttttgaat gcttctttcg taatatgatt ggtccctttc
     4081 gttcgatctt ccaatacgtc accatagtga taatgatcca tgatatagct caaatgacga
     4141 tctgattcac gatcacgttt gtaccgacgt tgccataaca cttcaaagtc tgctgttagg
     4201 cgaatcgtga tcacctgata tccgtatcta tctgctaatt cttggagtcg tgctttttgc
     4261 ttatcactaa aaggatactc ggacacaatc actttctttc ctgcttccat atacaaatca
     4321 agtgctttgt aatagaaagg ataaactttc gtcctttctt gttccgcccg ttcttccaaa
     4381 ttgttaaacc cgatggattc tgcatagtat tccttgattt catcgagcgt gatggcatac
     4441 atatctggaa atcgttcccg tagtttattc ataagatatg tttttcctgt accaggacta
     4501 ccagccagaa gaatgaaata ctttttcatc tcatttattt cctaagttaa ttggactttc
     4561 caagttcata aacagtccaa tcttatgctt gacaatttct tttactgctt catttgctgg
     4621 aatcacatta tggcgaagag acttgttgat ttctgtttca ctaaaggttt ctttagctac
     4681 tttcgtccag ttgactaaaa gctccgttcc aacattgaat ttgcggatcc cgttattgat
     4741 caattcagga taatcttctt ctttcacacc tgttcctcca tggataacta gggggacatc
     4801 aactactgca tcgatttctt tcagcaaggg aatgtttact tctgtttttg atttgaattg
     4861 tccatgattc gtaccgatag caatagctaa tgcatccacg cctgtagctt ctacaaattt
     4921 cactgcgtct tccggtcttg tatatacctt gtcattttca gcgacatgga ttccttcttc
     4981 tgtaccgccg attgttccaa gttctccttc tacggaaaca ccgtgcgcat gggcatattc
     5041 tactactgct tttgtcttca ggatattttc tttgaaaggc agatgggacc catcatacat
     5101 gacagaagta tatccgctgt cgattgcttc tttgatatca tcaaagtctc gggcgtggtc
     5161 taagtgcaag actgcatcaa ccatttcttc ttctgcaata gctgtcatta cattgactaa
     5221 cacatgcatg ccgatatatt tagcggtatc gacacttgtt tgaataatga tcggcgaacc
     5281 catttctttt gctgcgccta gcatagcagg aagcatttct aggttatgcg tattaaaagc
     5341 tccaactgta aaattcaact cttctgctgt ttttgttact tctttcaatg ttgtatacat
     5401 cttttattgc caccttatct ttttatttgt ttcctttgat cgttacttgt tttgcaattt
     5461 gtcgcatctc atccattttg cccatgtagt aatcgatccc ttgatcatca ccagcacgag
     5521 cagcatctgc acgcttctgg ttatagaggc tcatcagttt tttataccca tctccgatcg
     5581 tctgtttatt ttccaaactc ttttctaaat attggatggc ttgatcgttc acatacaatt
     5641 cagaaaattt aagtccaact tgttcataaa gtttcgctaa tttatgctta tcttgggtag
     5701 tggaaatctc ttgtttcaat tgtgtaatct ctgattgaat cttttcggtc gcttctggcg
     5761 atagggcttt ttcttctttt gccagaactt ttttcggttc tttttctttt ttcttaaaaa
     5821 atccaaacat cattttccct actttcaatt aaaatgcttt caaaactggg cttaagatcc
     5881 aggcatataa gagtgctgcg gctactgtat ctacatcggt tgctgtagca ttgttgaatc
     5941 ccatcgaatt aaagatcgtt actagcaaag cgggcaataa tgtaataaag aaaccatgag
     6001 caataccgcc aatgatcgct cctcggcgtc cgccaactgc gttgccaaat atccctgctg
     6061 tccctccggc aaagaaattg gttagcatac caggtaaaat catcgctaag ccgaacatgg
     6121 gcaaagtaaa catggcaata atcgtaccaa tcgttgtcgt aatgaaacct aaaatgacag
     6181 catttgggct ataaggaaag aaaactggac aatctaaggc agggatcgca tcaggaacta
     6241 atttcatagc tatccctcga aatgccggca cgatttcacc taacagtaga cgtacaccag
     6301 ctagcaatac atacacaccc actacgaatt ggatggcttg taaaaaggca aacatgatat
     6361 agttttgatc tcctgataat tcagaagcga atggttcgcc tgcaaataaa acagtgatga
     6421 tatatagcgg gaccatcacg accatgacag acaaataagt atcttgcaga aattcgaaat
     6481 gtgtcggcaa cttgatatct tcgatcgact ttttattttc gcctttctca ccaaaaagct
     6541 tagcaacacc ggcttcaaat aagtacccga tcgtacaaaa atgacctaaa gcaatatcat
     6601 ttgatccagt gattttacga atgatcggtt gcgctactgc cggcatagca atggcgaaac
     6661 aagctccgat aaaaccaccg accaagatca atacgatccc tcgcagaccg gcaaaatagc
     6721 caaagactgt cgtcatcgtt gccatccata aaattgcttg accagttaaa aaaatgtact
     6781 tccattttgt gaaacgagcc aaaataatat tgaatacaaa aatggctaag aacgtcagtg
     6841 caatatctct acctaaaccc aaatcattca ttgcttgacc attgatagat tcaatagaag
     6901 ggatgatccc ttgcatatga aatccttcag taaagatttt cccgaaataa gttaaacttc
     6961 caacgatgat gctcgaaccg gcactcaaca cttgaaatcc taggagtgtc tttaacgtgc
     7021 cagagatgac ttgtcctgct gatttcttct gcagtagtag acccaacata gcaatcaatg
     7081 caatggtgat cgatgcctga gtcaaaatat tttcgatgat aaaattgata acgcccatgt
     7141 tttgatgtgc accccaaaag ttagactaga aatctaatta aaaggggtgc tttttttgcg
     7201 gaaatataca tttgttttta agaaaaaagt agtttcagac tatttaaata acgaaggcgg
     7261 ctacaaatat cttgcacata aatatcaaat aaatcgtaca ctggttagac attgggtaag
     7321 gatttataac tatcatggtt gggaaggctt ggttggaggt ggcaaaagct acactacaaa
     7381 atttaaactt gatgttatag aatatatgga aacaaatggt ctttctatcc aagaaactgc
     7441 taaaaaattt aatatcggtt caaatagaac tctaagtaaa tggatagagc aatatgaaga
     7501 aggcggtgct tcttcacttg agagccaaaa aaggggcaga aaaattagta tgaattccaa
     7561 gctaaacatt cctaaaaaac ttaaagatga gtctcttgaa gaagaagtta ttcgtttaag
     7621 agcagagaac gcatatttaa aaaagttaga aaccttgatt caagaacaag atttgagcaa
     7681 gaagaaatca aggttaaaac aatccttcag ctcaaaaaag aatttaaatt gaatctatta
     7741 ctttcaattg cacaattagc gaagtcaact tattattact gggtaaaaaa attagataag
     7801 ccagataaat atagcaagat aaagcaagaa attacagcga ttgtaaaaga atcaagaaac
     7861 tcttatggtt atcgtagagt cactttagcg ttaaagatga aaggatatac aatcaatcat
     7921 aaaacagtta gaaaattaat gtcccaaatg ggacttacct gtcaaatccg aataaagcga
     7981 tataaatctt ataaaggaac agtaggaaaa attgccaaga atgtgttaaa acgaaatttt
     8041 tcagtagata cacccaataa aaaatgggtg acagacgtca ctgaattcaa gataaaagga
     8101 agaaaaatct atctatctcc tatacttgat ttatttaacg gagaaataat tagttatagt
     8161 atatcaacta gtccaacata taaactgatt gaagaaatgc ttcaacaagc aattaaaaaa
     8221 aagggaactg agggttcact gattctacat tcagatcaag gatggcagta tcagatgcca
     8281 caatatcaaa aaaaattaaa agagaataac attattcaaa gcatgtctag aaaaggaaat
     8341 tgcttagata attctgtaat agaaaatttt tttggtgtgc ttaaatcaga gtttttttat
     8401 cgagaaaaat tccgatcaat tgagatattt caaagtaaat taaatgagta tattaggtgg
     8461 tataataaca aaaggataaa actaaagtta aatggactat ctccagtaga atatcggaaa
     8521 cagtccatta aatagtt
//
LOCUS       DAYYCW010000110         8429 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000110 DAYYCW010000000
VERSION     DAYYCW010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.112"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..1655
                     /locus_tag="ACHAYA_002471"
     CDS             330..1655
                     /locus_tag="ACHAYA_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8606225.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1648..1998
                     /locus_tag="ACHAYA_002472"
     CDS             1648..1998
                     /locus_tag="ACHAYA_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606226.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1980..2165
                     /locus_tag="ACHAYA_002473"
     CDS             1980..2165
                     /locus_tag="ACHAYA_002473"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606227.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(3069..3344)
                     /gene="prgO"
                     /locus_tag="ACHAYA_002474"
     CDS             complement(3069..3344)
                     /gene="prgO"
                     /locus_tag="ACHAYA_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM8606228.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3316..4269)
                     /locus_tag="ACHAYA_002475"
     CDS             complement(3316..4269)
                     /locus_tag="ACHAYA_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8606229.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4881..6374
                     /locus_tag="ACHAYA_002476"
     CDS             4881..6374
                     /locus_tag="ACHAYA_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM8606230.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            6509..6805
                     /locus_tag="ACHAYA_002477"
     CDS             6509..6805
                     /locus_tag="ACHAYA_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8606231.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            6906..7757
                     /locus_tag="ACHAYA_002478"
     CDS             6906..7757
                     /locus_tag="ACHAYA_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606232.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            7778..7990
                     /locus_tag="ACHAYA_002479"
     CDS             7778..7990
                     /locus_tag="ACHAYA_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606233.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2932 a         1304 c         1579 g         2614 t
ORIGIN      
        1 gaaaacttct acatacaact attacaaaac tattaagaaa ctgaatactc ctattttttg
       61 tttttaagga aagttttttc ttttttgaat ttcttatttt tttctaatat aactaagcaa
      121 aactggataa tacgacactt attttcaaat tttcacaaga ctaatagtaa atatccattt
      181 aataaagtga tacaatgtgt tgattttaca aatcaacaga agaagtaggg aaataactta
      241 cctatgcttc tttttttgta catttaatcg attcttcttt acaaaaacga acgtatgttt
      301 gtataatgtg gaagaatgga gtgttattta tgatggatta ttcaaaagag ccagtaaatg
      361 attacttttt gatcgatatg aaaagctttt acgcaagtgt cgaatgtata gaaaggaatt
      421 tagatccatt aacgactgaa ttagtcgtca tgagtcgagc ggataataca ggatcagggt
      481 tgattttagc ttcttcacca gctgcaaaga agaagtacgg tatttctaat gtcagtcgac
      541 cacgagatct accgcaacct tttcctaaaa cgttacatgt cgttccacca cgcatgaatc
      601 tctatattaa gcgaaatatg caggtaaaca atattttcag aaggtatgta gcagacgaag
      661 atttattgat ttactcgatc gatgaatcga tcttaaaagt gaccagatca atgaatcttt
      721 ttacgactga aggaacaaga agtcaacgga gaaagaagct ggctcaaatg atccaggaga
      781 atattaaaga agagctagga ttgcttgcta cagtaggtgt cggagataat cccttattag
      841 ctaaacttgc tttagacaat gaagcaaaac ataatgatgg attccttgct gaatggacgt
      901 atcaaaatgt tcctgataaa gtatggaata ttcctgaaat gacagacctt tggggtattg
      961 gatctcgaat gaaaaaaaga ctcaaccaaa ttggtatcat gagtattaaa gacttagcta
     1021 attggaatcc atacactatt aagagccatt taggtgtgat tggcttacag ctgtattttc
     1081 atgcgaacgg gatcgatcga acagatatag cgatcccacc agaaccgaca aaggaaaaat
     1141 catatggtaa ttcacaagtg ttaccacgag attataccag gagaaatgaa atcgaactgg
     1201 tggtaaaaga aatggcggaa caagtagcta ttcgtatcag gcaacataac tgtaaaacag
     1261 gttgtgtcca tttatacatt ggcacgtcga tcttagaaac gagaccaggt ttttcacacc
     1321 agatgaagat ccctataaca gataatacaa aggaattgca gaattactgt cttttcttgt
     1381 ttgataagta ttatgaagga caagaagtaa ggcacgttgg aataacctat agcaagcttg
     1441 tgtacactga ttcgcttcag ctggatttat ttagtgatcc acaaaagcaa attgacgaag
     1501 aaaatttaga taaaattatt gacaagattc ggcaaaaata tggttttacg tcgattgtac
     1561 atgcttcttc tatgttagaa ggcgctcgta gtattactag aagtacattg gttggtggcc
     1621 atgcaggcgg cgcaggaggg cttaaaaatg attgattata atgatcgtgg acgtttaaag
     1681 tgggcaggtt tttatttatc tgatcatact gaaaaaattg atgccgacaa tgaacaacga
     1741 gccaatcaaa atttggcaaa agaacaaatg acgactgaag aggttgcgga agtattgaac
     1801 gctgcagtca taaaaaatag atcagtatgt attcaaaaag aagaacgaaa cgctgaagga
     1861 tactatccag aagatattgt tagcaagatc ttaggtagtg atgagctggg gatctacgtt
     1921 ggaaaaaaca aagttcacta cgacgaaata cgtcatatcg aattttacca ggataataaa
     1981 tggggtcgat tagaatgaac attatccagc aatatgagct aaagtatata acctttgatc
     2041 agctatcaga agagatttgg ggttatggcc agcgtttgat aaatgaggtt ggtgttgaac
     2101 gcttctcttt ctatgtagaa gctgcagcag gataccataa atacgaatac tatattggct
     2161 attaaagtgc aataaaatta tatttctttt atgttttaca ttttgtatca attctgataa
     2221 aattacctat aggtaattaa aaaatataaa aaaactagtt acaaccttag gacgttgtaa
     2281 ctagcttgaa gcatactaga gctaactaaa atagcactag aaagcaatgt taaacccgtc
     2341 caacaaactt gatttaacat tgagaccagc ttattcagct aaatatagtt gaataagctt
     2401 cttttgtttt aaaaaaacaa aaggtgtact acgaataacg caagtaaaag aaatactatt
     2461 acaaccagta ggctacaagt aagtattact aaacattgcg tagttagaac ctttgctcta
     2521 ttgtctaaag ccacaaaatt agagatcagc tttatgatgc taagagcgag ctgcatccat
     2581 gcggacatag gttcttttga cactattaat tctccttttt aggataaggg aaattgctag
     2641 tagttttttc attagcacca tcccccttca tttgattgag tctagtgctg ctgtgatagt
     2701 ttgctgttta atatgcttac attcttatta taacataaat gaaacaaaca catggttaga
     2761 ggtcgtatca atcaaaatag tgtctcacaa acgatttatt acttactcct gctgactaac
     2821 ggactgttct tcagtaactg taactatatg tttgttaaaa ccaccattac ctgtagataa
     2881 aaaccaatat atttactata ctcactatat taaatatatt tagtatagta aaaactagaa
     2941 acaataaaat ttgtgaatgt tgtatacccc caaacaatac acatattttg aaggattagg
     3001 attaataaag ctaaaaatat agtaagtata ctaaatatat ttactatatc tagtatactt
     3061 actatatttt aatcttttga tcgtctatta tatataatcc gttcctcgtc gtttaaagta
     3121 tcaacatagg cgtccattgc aagtttgact atttcataca taggaatatc ttttgcataa
     3181 ctaagatccc gaatggtttt tagtacggga ggatctactt gaactggttt tctttctttc
     3241 gcagtgtact ttcgttcccc taactcgaca ttaatatcat ctttaattga aatatcttca
     3301 gcaccaattt tcttcttatc tttgttcgtt ggtaataatg ccatttagtt taatctcctt
     3361 tccaagaggt aggatatgat tggccattga gtcagcatat ttaggtgtat aatcaaatcc
     3421 ttcaatatca ccatgttgtt catagtattc aattctttct tctaattctg taaagatgtc
     3481 tgcaaaaata gcccaccatc ttcgatctac ataatcattt aattggatac cattctcacc
     3541 atacatttct aaccgatccg agcctttaac aatgctttta aaaatgtttg attcaccata
     3601 cttatcaatt gttttttgat agtttgcgtg ttgagaggaa cgtcgcactg agaaaagaac
     3661 aggcaaaatt ccaatgattt gaagatgaga ctcttcaaaa ttcgtgacaa tcggaactaa
     3721 aactttatta ataaaatctt ctgttccttc cattgcgtac cgttttaatt cttgcattgc
     3781 aataatataa tctgcagcag ttaagaatgc acgaaccaca ttatctgttg atggtgggca
     3841 gtcgataaag atataatcga aattttttct caattcttct aaaggtttaa tgaaggccaa
     3901 tgatctctta cgacgatcag gatatttttc ggacaagtat tcattaagtt cttcagatcc
     3961 aggagagcca gccataatat ataaatgtgg acttacttga gtaatacctg aaacccagtc
     4021 cccttttttg attgctgcag acatggatcg atcaaagcta gtaatttgat acgttgatcc
     4081 aatgttggaa gtagtatttc tttgttggtc ataatcaatt agcaacacgt ctttttcaaa
     4141 aagttgacta gaaacaatcg ctaataaatc attgtctgta gtcttaccaa tgccgccttt
     4201 tctacctgta agcattacaa cttttcctag tttatctgat gttttaaatg cagtccgtaa
     4261 gttttccata gttccctcca atttcctata ctaaatatat ttagtatact tcatatactt
     4321 aatatattcg atatatctaa tatactccaa attgataaga attcagactt tataaggaat
     4381 atagtggata tactaaatat atttgctata ttcagtatat cagatatatt caaaatgtca
     4441 aaatatattt gtgcgttttg cgttgacaat ttagagtgaa aagaggtaaa cttaattcta
     4501 tagaaaccac agataattca ctatttacaa agtagcaaaa gcagtggtac tctttaaaca
     4561 gaaaaattac ataaaaaaaa gagacggaaa taattgattt tagcagaatc aattatttct
     4621 gaatcacatg gttaggacat gtgttcacgc tcgtctcaag aatatgtaag tagctaccaa
     4681 ctacttatat taaaagttga tcggatacct tttccgattg cctttattct accaaaaaaa
     4741 ggcgagcaac tcaacagaag aacgtttatt tagtgtactt aaattaaata ttcttaaaca
     4801 ctgaagagag aacacatact atggctgaga ttaaagctaa actgtgttct ctttttgttt
     4861 gagaaaggac gttacaatcg atgttgagcg aaatttattc agctattctt aaaaacggct
     4921 tacgtacata caaatatcga aacagcacca tgaaacctgc aggtcatatt gattccagta
     4981 ggagtggtgc tatctttgcg taccgtagca agcaattaat gaatgttggg cgtggtatgg
     5041 tcattacttc ggaagaagct attttagaga acgaaaagaa actaacgcat tggacaccta
     5101 atacctatcg ctttggtact tatgcggatg attatcgaac agtggttaaa ggtcattctg
     5161 agaaaaactt aagtcaaatc aacacatttg tagttgatat cgattcaaaa gagaaccatc
     5221 aaggcgaaat tattttagcc tgtttagatc aagtgggcta catgccaacg ctaattttag
     5281 aatcagatca tggttatcaa gtttattttg tactaaagac accaggctat gtcacaaaga
     5341 aaagcaattt caaggtgatc gatgtagcaa aaaaaatttc aaaaacaatt cgtctacagt
     5401 tggctgaaaa attgtccgga gtggatctag gatgtaacca cttcggaatt gctcgattcc
     5461 cacaagaaaa taatattgtt tttaaagaaa tgaactatca gtattctttt gctgactgga
     5521 ttcaatggtc gatgaagctt gaatcagatc aaaaaacaaa agaagatcga gaacgaaaac
     5581 taattgtctt tcctgagaaa aaagaaattc gtcaagtaga tgaaccgtgg ttcgatatgt
     5641 tacttcataa agcaaacatc gtcggcggag aaggacgtct tggtcgaaat aatgtgattt
     5701 tcacgctttc tttagcttat tactcttcag gttatgcatt ggatacttgc gaatacaata
     5761 tgttcacatt caacgaacgg ttacaggaac cgctatctga agcagaatta gtaaagattg
     5821 tacgtagtgc ttattctggt aattatcaag cagcaagcag agaattcatt attgaattgt
     5881 gccagcaatg ggtttcacca accttaaaaa ataatgatct gttcattcaa cgacgtggct
     5941 ggtggaaatt taaaaagcca cgagatcaaa gaaaatatag tcataaaaat gaatggaaag
     6001 aagatctatt ggcttatcta agcgaaaaaa gtcttgctag tgagccttat ttgaacctgt
     6061 caaaaaaaga attggcttca caactaacga tgcccaagcg aacacttgat aagatcttaa
     6121 atgagttacg agaggagaat aaagtcttct acaaggtaac acgaggaaga aatggcggaa
     6181 tgatcattgc ttcagtcaaa acgttgttcg cgagggttat tcaactaagt aaagaaaaga
     6241 gactagatta ttcaacaagg atcacagagg tatttggact caacgagagc ttagtagaag
     6301 agacatttag acaattatca acccccttga accctcttaa agaagtgaat ctttttgaag
     6361 tagatacagg ttgaaaaata aaaggtgcaa attgccttat acaatatatc tatgatactt
     6421 ctgtaggtgt tgctcccttt gcctagctac gtgtaggtgg tatacttata actgcgtatg
     6481 cagtcttatg tatcatggag gaagttgtat gtcgttaaaa aattatgtct attcacatcc
     6541 tgttaatgtg tttatcattg gtcgtttggg attatctgtg gaagtgttct gtgagcttta
     6601 tggttttaag caggggactt tgtcttcttg ggttaccagg gaaaaaacag ttgcttcttt
     6661 gccgattgag tttcttcatg ggttgtcctt agcttctggg gagactatgg atcaggtata
     6721 tgattgtttg tgtgtgttag agcaggagta tattgagtat aaaatagcta acgagttaag
     6781 aaaaaggaag aagtatatcc agtagtagtt tttatttact taaaaaattg gttcattgag
     6841 caacctatcg ctgctaaagt agcaaaggaa aacgttaaag gaaagaattt agggaagaaa
     6901 tcgaaatgaa atcggacgac tggattgaac acacctggct tcctctgaaa agaaatagca
     6961 aacgaaagaa attttgggta ttgcaattat ggccattagg aaaactttgt agttacgggg
     7021 cacgacttat tcttgattta accgtttact cgattggagt atttggaacg gcttattact
     7081 tgataaatga tcccaatagg attgcagcat cagacatttt tacagtgcta atgacgttga
     7141 tttttgtcat gctattgtct atttttactc aattagtcat gcaatttacc aggcaaaaat
     7201 taacagcagg aaaacttttc aaaagcctat tcgatttaat tgttgtaaat gcaattctag
     7261 cgactttaat aaaacaaatt gaacaatctg gaagaacaac aaaaggagtg aaggattttt
     7321 tattgacgtt tattcacttt gtaaatggac acatgtattt gtttgaagtg ggtattattc
     7381 tcttttttgt cgggtatgta tccacgaaac ttattaaacg ttcaattgaa aaagaaattg
     7441 aaattgacct gattccgaca cttcttggtt atgagtacaa tgttgatttt ggaagatatg
     7501 acgaattgat caatacggat atcgttcaca cgagtatcca tgaatctact gttaagggca
     7561 tcagatctat tttaaaaaat ggccaggaaa tgaaagcaat agatcgaaga aaagagatct
     7621 acaattttta tgaagtcgaa gcaagcctac gatatggcat cggaaaattg tggttagatg
     7681 gaagtaatat tcgtcaaaac aaaccataca ttttttggaa cagtagtgat ttagaacttc
     7741 caatgaaaga taaataatta tggagaatcg tttgaaaatg attcctctat acaggattgt
     7801 gcttatagtt ggcatggagt taaaaacctt aaagaaggag cagttattca aagtgaaaga
     7861 acgtcataat cagttactat gtagtaaaac gggtacgatg aacattgaat accgaacaga
     7921 atatttagaa ttaacgaatc aaactgattt tttgttgatt cgaaagcaac aagagcaaga
     7981 aaaaaattaa tggctactta tagccaattg attgatgaat gggaacaatt caaggaatct
     8041 aaaattccag aacgtctcta aaagccttta tttgtaaaga gcataaggaa acgtgggaac
     8101 actttttaaa aaaagtgttc caataggctg tgcctattac gtttctcaaa aataaatgtg
     8161 ggaacaaaat atttaaaaat aggatagtga aaggatagtg aaggatagta aaaggagggt
     8221 agtaggatag caaaaaggat aggctccttt tcgctatcct ttttaaattg gaggaattaa
     8281 tatggaagta agaataagaa atgtggatga gaaaatcgta agagaaattg atcgaagatg
     8341 tgacgaacgg ttctgttgca aagttttaaa tctactatca aataaggtag aataatagaa
     8401 aaagatagca ggaggaatga cgatgaatc
//
LOCUS       DAYYCW010000111         8417 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000111 DAYYCW010000000
VERSION     DAYYCW010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.113"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(102..620)
                     /locus_tag="ACHAYA_002480"
     CDS             complement(102..620)
                     /locus_tag="ACHAYA_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8606234.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(622..1239)
                     /locus_tag="ACHAYA_002481"
     CDS             complement(622..1239)
                     /locus_tag="ACHAYA_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8606235.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1236..2090)
                     /locus_tag="ACHAYA_002482"
     CDS             complement(1236..2090)
                     /locus_tag="ACHAYA_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8606236.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2087..2998)
                     /locus_tag="ACHAYA_002483"
     CDS             complement(2087..2998)
                     /locus_tag="ACHAYA_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8606237.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3039..4373)
                     /locus_tag="ACHAYA_002484"
     CDS             complement(3039..4373)
                     /locus_tag="ACHAYA_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8606238.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4581..5411
                     /locus_tag="ACHAYA_002485"
     CDS             4581..5411
                     /locus_tag="ACHAYA_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFM8606239.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVDGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5655..5904)
                     /locus_tag="ACHAYA_002486"
                     /pseudo
     CDS             complement(5655..5904)
                     /locus_tag="ACHAYA_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6095..6289)
                     /locus_tag="ACHAYA_002487"
     CDS             complement(6095..6289)
                     /locus_tag="ACHAYA_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606240.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6794..6979)
                     /locus_tag="ACHAYA_002488"
     CDS             complement(6794..6979)
                     /locus_tag="ACHAYA_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606241.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7829..8170)
                     /locus_tag="ACHAYA_002489"
     CDS             complement(7829..8170)
                     /locus_tag="ACHAYA_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8606242.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8170..8400)
                     /locus_tag="ACHAYA_002490"
     CDS             complement(8170..8400)
                     /locus_tag="ACHAYA_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8606243.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT         2743 a         1632 c         1411 g         2631 t
ORIGIN      
        1 agttattaag acaaatgaat aaacaactac gctttcctcg aaacaaaaaa ataaagcaca
       61 aatgcgcatt tacgcactca tgctttattc gtacaattaa ttcagataac ggcagcgcat
      121 aggatgccaa taacacttcc taaaatagct cctcctaaaa tgtcagtagg ataatgtaaa
      181 agtaaaaaaa tcctcgaata agcgatcaaa caagcaaata aaaaggctaa tattcctgca
      241 actggactta attcgaacaa cgcagctact gataaaaagg ctagcgctgt atgtcctgaa
      301 ggaaaagaag aagtcttcgg tttacttgtg taaaggacaa gccgacgatt tttttcataa
      361 ggtcttttcc gtcttgtcgt ttttttgata aaaagcaaac caacaaaaaa atagatcaaa
      421 ctgccgataa gaatggctag accatattct ctagtatcag gtatcaagac taaaacagca
      481 gcaaggagca accaaataaa accaccatta gagatattac tgataaattt caaaaaattg
      541 atttttcgac tatccgtttt ccgttgcttg gcgaacttgt tgataatact atcatcgatt
      601 ttagcaatca aacgcaacat gttattcctc ctttttaaga accttttctt gcgtacgatg
      661 gataaaaagc aggacttgaa aataaaacaa gaaactgaaa cctaataaag caaaatagat
      721 tggtcctgca aaccagaatg cgacaagaag gatcaacgtg cctgccaaat agaaaagcgc
      781 gcttttgaaa tcattaaaaa acaagaaaaa tgcagtaatc caagaagctt ctttttgttc
      841 cagttgaagc aagattttgt aactaaagag aagcgcgctt aatagaagca aaaacaacgt
      901 agtatacatc aatgtatagc caattccccc accccaatag cggaaaatct gataatcgat
      961 caatagaaga agaaaataaa cacttgctaa actatcttgc tttccataaa gagagaagtt
     1021 ctcacgaaac tttttccaga atagtgaacc gagctttttc tcttcatttt ttttgtaatg
     1081 ataaatcata tacagaactg ttcgacttgc agttcctaat gtgaaaacag gcaagacgca
     1141 caggaaccat agtagctgta aatggataag acgtggaatc cattcaatga tttctaagaa
     1201 ataatcattg gcttttttac caatcgatcg attcatcatc cttttgaacc tcctgtcaaa
     1261 tccatccctt caatgaaata acgttgtgca aaaaagaaca gcaaaatcgt cgggatcaag
     1321 acaagtgttg ctcctgccat cagataattc cattgtgtcg ttgcttggtt ctgaaaaact
     1381 tgcaagccaa tctgtaacgt gtatttttct tgatcttgga tatataacag aggacctaag
     1441 aaatcattcc aagctgcgtt gaaggaattg atcgcaatgg taatcaaggc aggtttcgtc
     1501 aatggaagca tcaaatgact ccaaatatac aaatgattcg ctccgtcgat ttcagcagct
     1561 tcgatcaatt cattattgat cgataagtaa aattgacgca tcagaaatat attgaacgca
     1621 ttgccgaaga acgatggaac gatcaggggc aaataagtac ctacccagcc gatcttggag
     1681 aataatacat attgagggat catcgttaca aaccccggga tcatcatcgt agaaagaacc
     1741 aaagcaaaca tcagcttttt cccaggaaaa tcaagcttcg caaatccata agcaataaaa
     1801 gaatttgaaa ggacattacc gatcactact agcacagtga tgaataatgt atttcttgca
     1861 tagcgccaaa aaggaaaggc ggcaatcgta tccagatagt tttcccatac gattttttct
     1921 gggaaaaagg tcggaggata acgaacgatt tcttccattg gtttcaatga agtaaacacc
     1981 atccaaagca atggaatgat caaaatgatt cctattactg tcatgaggac ataacttagt
     2041 attttttttg tttgccgatt cacactggtc tttttgaaat aatccatcat ttgccgtcac
     2101 ctccagaata atagacccac ttaggtgcaa gcttcaaatt gacaaacgtc agaaccaaca
     2161 cgatcagaaa caggatcatt gacatagcca tcgcataacc catatcaaaa gctacgaacg
     2221 ccttgttcca catatgcaga ttataaaaaa gcaaagaatt tgcaggtccg cctgttccat
     2281 ttttcgtcat gacataagct tcttgaaaaa tttggaaaga accgatcgta gaggtgatca
     2341 catcgtaaaa aataatagga gtgattaatg gcaaagtaat acttttgaac tttccccaaa
     2401 cgcctgctcc atcgatttct gctgattcat ataaagatct aggaacattt tgtaatgtag
     2461 ctaagtaaag tagcattgct cctccagaac tccatatttt catcaaaatc aacgcaggtt
     2521 tcgtccattg tggatcaaac agccaagctg gtccagggat atggaaccag cccagaaatg
     2581 tgttgatcaa tcctgcagat ggagaaagca gctgcatcca taagatgtag actgctactc
     2641 ctgataagac tgccggtaaa tagaaagctg tacggaatac actgattcct ttgatttttt
     2701 gattgagtac tgtggctaaa aacacactga aaatcgtcgt cagcggtaca ttgaagatga
     2761 cgaaataaac ggtgttgtat aacgatttcc aaaataaatc atcgtgaacg aacattcttt
     2821 tgaaatttgc tagaccaatg aaattcatct tggatgtcaa attataatcg gtgaaactac
     2881 caataactga aaaaagcata ggccctagca taaataccaa gaacccgata ataaaaggcg
     2941 agataaataa ataggcccat cttgcttctt ttttctttcg gatagataaa cgcttcaaat
     3001 tttcttgcct cctctgtacc tagacagtca aaaatggatt attgattttg cttgactaag
     3061 ttttctactg ctttttgtgc atctgccaat ccttcttttg gtgattttgt tcctaaaaga
     3121 atctggtcaa tctgttcatt cactaatgtc gtgtaatccg gagcatctaa aggaactggt
     3181 gtgatcaccg tatttttaaa gttttcgtcc gccatttgat agatcatttt tcctttatcc
     3241 gttaggttgt cattattcac taaattctca ttggctgtct tgctcgccat gatatcaaaa
     3301 cttttttcgc cgaatttttc ttgtacttct ggagaagaaa gatatttgat gaattcataa
     3361 gaggcctcag gatcttttgc tccgtatggt acttccaaga cgaatccgcc accccacgac
     3421 caatggcctg aaccttcttc tttttctggg agctgcatca cgccaaaatc aaaatcatcc
     3481 ggtgcatttt ccatcaaact agagtaataa ttgatatttt gggcacgcat ggctactaat
     3541 ccggaaataa atggatcaga tacaccagac ccaaattcgg cttctaattt attgatcgta
     3601 tttttcccat aataatcttg ccagtcttgg atccactcca aggcttccac tttattttta
     3661 gtatcaatct tgactttttc atctttgtca aaccaactag tcccgttatc tgcgtttagc
     3721 gcccatacat cagcccctaa attccataat ggataaaaac cgatgcgtgt aaattctttt
     3781 ccttcttttt tatctagttt acgtgctgtt tcttctaatt catcccaagt tgtcggtaga
     3841 tcttgttcac tgattccagc ttctttaaat aactttttat tataaaaaat gacttgtgta
     3901 tctgtattga atgggatcgc ataaggatca ccttcatatt ctactgtatc ccaaagctgc
     3961 ggataaaaat ccttagaagt gtctttttct acatatttgg agagattagt tgcttgctgt
     4021 gcttctgctc tttgggcaac ggaatttatg tcctgtacaa taacatcggg tgcatttcca
     4081 gctgttattg cagataaaga tttagtccag atgtcacccc atggctgata cttataattt
     4141 acatggatct cgttttgtga ttgattgaaa tcctcaatga tttcttcgat agcttctttt
     4201 cgtgcaccgg atccccaaaa ggaccagaaa tcaatatttt ttcttccatc acttgttttt
     4261 tcagaagaat ttgctgcact tgaactggca tcgcttgcat tacttgatcc gcatcctcct
     4321 aataaaaaca atgctgaact tgccagcaac cctgtaaaca ataatttttt catattttac
     4381 ctccaaattt taatgtaacc gatgccggtt ttaatataac gatatcattt tgttctgaaa
     4441 ataacaattt ataaggtatg attctaaaaa aattattaat gataccaata ctttatataa
     4501 ttcgcgagcg ctttctttga attttttaat ctttcgtagc agaatgagaa tgtattaaaa
     4561 atagattagg aggaattttg atgtttagac accagaaaga attgcagttt gaagcaaaac
     4621 cagatcgtcc gaatccatta atcgcgaaat atctgcagga gctgatcggc ggccaatatg
     4681 gcgagatgag tgtagctatg cagtacctgt tccaaggctg gagctgccga ggagaagaaa
     4741 aatataaaga tatgctgatg gacattgcaa cagaagaaat cggccacgtg gaaatgttgg
     4801 cgacaatggt tgctcgttta ttagagggtg cgcctgttga agatcaagaa gcagcgatgc
     4861 aaaaagatcc aacaatcggt gccattatct ctggaatgaa tccacagcac gcgatcgtag
     4921 atggcttagg acctcgtcct gctgacagcg tgggcaatcc atggagcggt gcctatatca
     4981 ttgcgagtgg caacttgtta gcagacttta gagctaattt aaatgccgaa tcacaaggaa
     5041 gacttcaagt agcacgtatt tacgaaatga tcgatgatcg tggcgtaaaa gatatgctgt
     5101 ctgttctcct tgcaagagat agctatcatc aaaatctatg ggaatgtgca gtgaaagaat
     5161 tagaagaaaa agaaggttca atcttagtcc caagtacttt cccgcgtgac aaagaacgaa
     5221 aagacattgc ttatgacttc tataacttct cagagggaga ccaaagtagt gaaggttctt
     5281 gggctagtgg caaagcactg gatggcgaag gcgaatatca atggttgaaa gaacctaaga
     5341 tcgaaggcgc tgcaccaaaa ttagaaccag taacaccaaa aatgtatggg acacctccat
     5401 tcaagaaata aatatctacc cgtagaaaca cccgtttttt gttccacacg ctttgtgagc
     5461 aaaactgact aaagtcatta atcataacca cccttcaaaa gggtggtttt ctctagagct
     5521 ataagctcaa atcaccggcc agcgcctaaa gacgctggct ttcatatttt atgttcaagc
     5581 catattgcct ttgccagttt tgcccacctc ttaaaaggtt tatgtactac tccttagccc
     5641 ttaaaaggat cgtcttactc tcgtacactc agcttatcta acgcactatc atgtttttct
     5701 tgttcttgaa tgtacttttt gatggtcgct tcattttgtc ctacttttgt cacatagtat
     5761 ccttccaccc aaaagtgtcg atacccaaat ttatatttta gatttgcaca atctcttgat
     5821 gatctcctga atattggctc gatattgatt ataaattatt tttcttctat actttggtgt
     5881 gaatacaatg tggtatttac acatctactt tgtacgggct aaactatttc cctttattgc
     5941 tccacccgca tagcaagtgg ttttgtattt caatcgctcg gcgcgctcaa ttaggtctaa
     6001 agacaataaa tctaaaaacc cgctgcttca ctgacagaaa caacgggttt tcagaaaaaa
     6061 atgaaatgaa aaagatctct tacggcaatc tattttattt ttttgatttt ttataaatgg
     6121 aaaatgcttc aagaagtcca agaaataaag ccgtataaaa acaagaacct atgaaatctt
     6181 tatttattag atattcaaca gaaatcccga taacactagc caacaatata tttatcagaa
     6241 gtgtaaaata aggatgcttt ttatagaata gaactagtgt tgttttcata gactctcctt
     6301 tcttcatatt tccaattaat cacaaattca cggcattact agaaaaatta aaggggcgca
     6361 gaagtggaaa aatactcttc gactttccct acaaaccagg ccatcttgtg tttaataact
     6421 ataccaaatc taaggtacct ctaaatctaa attccatttg tggcggtgac ctccaatttt
     6481 taactcttct atctcccatc cagtatacat aaccttgata cttcgaccac ttatatatcg
     6541 aacacacaaa tacctatcca ttgcaggagt actacctaac ttagttgcta cttgcatcta
     6601 aataacagct gaaaagatag gacttactct tagtaaagta tctgaactat taatttttaa
     6661 actatcattt ttatcctcaa ctacatgcac atctgttgca acttcacttt ctgtctcatc
     6721 cagataaatg attaaggaat cgggaattgc aatatcttca gctgacgctg gaatagttga
     6781 gctaaattag tcatcatatc tttattataa aataaccaac agcaaaaaat acaatgaaaa
     6841 acaaacttgg aactaatgta tcattaataa atgttgaaaa attcagcaac tcattactgt
     6901 atgattttag cagcccaacc aacatatacc ctataaaagc taataagatg gttaggaatc
     6961 ctttagaatt tttactcatt catagacact tccttaaata acttttatta tagaaggtat
     7021 tgtaaataat aatattgaca aaaagtgact caaaataaaa aaaaccttac attatactgg
     7081 aatgaactgt attgatagtg tacctaaaag ctctactgac ataagatcgt gtaatcggta
     7141 aagtaatttt attaattata cgggatgtaa tcaattagag aataaactaa tcataaaagg
     7201 tacttttaca ttcataattg tcgctttgag attctacttt ggttcactat ctgacccaat
     7261 tgcacatttt cgatcattac aagtattggt gtcgttagaa caactaaata aaaactagcg
     7321 gtagattttt cagaatctat gctaatttct gtatggatgt tccagtgatg gcaaatttct
     7381 cttttcattg tttttcttgt cgcatgatat gcctttttgt gttattcagt taaactatag
     7441 aaattctttc ctagacagat cttgatccca aattttacgt ttttgagcat cgatatagtt
     7501 taaaatgaca ttactcatat ctaaagagtt tagatttttt aggactaaaa ctgtcagttg
     7561 ttctttttct tctttctgtt aaagtattgt cgcgactttt cgtatacgtt aagatatgtc
     7621 gtgaaaaaac aagttttgtg acacaaaaaa acgaatgact tgaggaaatc acctcaatca
     7681 ttcgtttaaa atgctatata ttctttagtt taagatacgg aaattaatgc catcgaccac
     7741 actagtagaa tgaacgacat ttacttggcc tttttttaat ttcgttatgt ttagcatata
     7801 aaaatattat agagtttact tacaatgctt aaaacatata ctgtataatc tgatcaattt
     7861 tagccatgtc ttgcaatggt aagttttcta tttttttgag ttttcgttcc ttaaaatcaa
     7921 gagattttaa ttgtgtaata attacttgtc cttttatatc tagctcttca ggtaatgagt
     7981 aacgtgtcgg taaattcttt atagtggaag taattggaca tatgaccgaa aacattgttt
     8041 tacggttaaa ttcataacga gagacgacta gtcctggtcg tcttttttga atttcttttc
     8101 ctacggaggg atcaaaatca atccatacaa tatcaccttt tttaggaata taaattcttt
     8161 cttctgacac tataaaattt ctctcccttc aggagttaaa tcttcccatt cgtctttctc
     8221 ataatactcg gcctcttccc caccactaaa tggatcttca atttttggaa ctaacgtaat
     8281 tgttccatca tcagaataca caacgatata ttcttggttt tctgaaggtt tctttccgtt
     8341 atcagaaggt aaagttacca ctacagaact tccttgtaat ctagattttg taacaagcat
     8401 tatcatcact cccttaa
//
LOCUS       DAYYCW010000112         8377 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000112 DAYYCW010000000
VERSION     DAYYCW010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.114"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            165..1037
                     /locus_tag="ACHAYA_002491"
     CDS             165..1037
                     /locus_tag="ACHAYA_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8606244.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1034..2992
                     /gene="nagE"
                     /locus_tag="ACHAYA_002492"
     CDS             1034..2992
                     /gene="nagE"
                     /locus_tag="ACHAYA_002492"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8606245.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3324..4175)
                     /locus_tag="ACHAYA_002493"
     CDS             complement(3324..4175)
                     /locus_tag="ACHAYA_002493"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8606246.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            4298..4765
                     /locus_tag="ACHAYA_002494"
     CDS             4298..4765
                     /locus_tag="ACHAYA_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM8606247.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(5028..5510)
                     /locus_tag="ACHAYA_002495"
     CDS             complement(5028..5510)
                     /locus_tag="ACHAYA_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8606248.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5610..6473)
                     /locus_tag="ACHAYA_002496"
     CDS             complement(5610..6473)
                     /locus_tag="ACHAYA_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8606249.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            7071..7946
                     /locus_tag="ACHAYA_002497"
     CDS             7071..7946
                     /locus_tag="ACHAYA_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM8606250.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(8091..8243)
                     /locus_tag="ACHAYA_002498"
                     /pseudo
     CDS             complement(8091..8243)
                     /locus_tag="ACHAYA_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
BASE COUNT         2595 a         1691 c         1494 g         2597 t
ORIGIN      
        1 gattacgtta acaataattc gtttcatgtg actagatcat actaggcatg aaaaatctct
       61 gaggaacatg gtttccctag gaggtttttc gtgtctattt ttgtttggcc gcaatcaact
      121 ggtctagcaa acgtgaaaac caaatcttca aaaagggtgc gcgcatgaaa atcaaaaaga
      181 tctttaatca aaatgcagta ttggtagatg acggtggaca ggaaaaagta gctattggaa
      241 aagggattgg atttgacaga aaaagaaatg atttgatttt tgatcgtgat gtcgaacgga
      301 tatttgtcat ggaaccagaa ggtcaaatca agttgcaaaa tttacttaat cagattgatg
      361 aacgcttttt atttgcagca gaaacgatca ttgatcatgc ggaaactgtt ttgatggaaa
      421 agttgaatga acatgtattg atcgctttga ccgaccacat tgctttttct gcggagaaca
      481 ttaataacgg tatcgtcatc cgcaacaaac ttttaagaga aatcgaagtg ctttacagtg
      541 atgaattcag tattgctcaa tgggcagtag agtatctgac aaaagagctt gggattccat
      601 atacttatga cgaagcagga tatatcgcaa tccatattca cagcggacga agcggtcgga
      661 acaataacca tcgcagtatc cgtgaagtga cgattgtatc tgacatcatc catttagtgg
      721 aatcagaatt ggaaacagat atccattcag aaccattttc actgaattat tcaagactgg
      781 tgaatcattt acgactgctt ctacaacgaa cccatgcgca acaatatgct gttttagaca
      841 cggaaatcgt agaaatggtc aaacgaaaat acccagaaag ctataagatt tctaaaaaaa
      901 tccgtgtgtt gctgaccaaa gaataccagc tcgcgatcac gaaagaagaa ctgggttact
      961 tggccatcca tatcgagcgt ttaagaagcg caatcgtaca aacaaatgtt aacaatcatg
     1021 aggaggaaaa aaattgaaag catatctaca acgaatggga cgttcccttc aacttccagt
     1081 tgcggtctta cctgctgctg ctttactagt agggatcgga aactggtggg catcttatag
     1141 taacgacatt gtcgctcatt ttttacaagc tggcggtaac gcagttttag gtcaattacc
     1201 tttgctgttt gccgttgggt tagcactagg gatgtcagtg gacaaatcag gtgctgctgc
     1261 cttggcaggt ttggtcgcat ttgaagttcc agtaaatgtt ttaaaaactg attcagtagc
     1321 tactttattg aatatcaaac cagaatcagt tgatccatct tttgctcaga tcagtaacgt
     1381 atttattggt attattgccg gattgattgc ggcagctctt tataatcgtt tccacgaagc
     1441 aaaattaccg atggctttat ctttctttag cggcaagcgt ctagtgccaa tcttggctgc
     1501 ctttgtcatg ttgctgatca gtgcagtgct actgctcgta tggcctccgg tttactctgc
     1561 tttagtttct tttgctaaat tcattttgag tttaggctgg ggtggtgcag gtctttatgg
     1621 cttcttcaac cgtttactga tccctacagg attgcatcaa gcaatgaact ccgtgttctg
     1681 gtttgacgta gcaggaatca atgatattgg taaatttttg gcttctgaag gaacaaaagg
     1741 cgtaacagga atgtatcaag ctggattttt cccaatcatg atgttcgggc ttccagcagg
     1801 agcatatgct atttacagaa atgcacgtcc agaaaacaaa gcaaaaacag cttccttgat
     1861 gttagcagca gcttttgctt cattctttac tggtgtaaca gaaccacttg aattctcatt
     1921 tatgttcgtt gcttggccat tatatgtctt gcatgcaatt ttcactggga tttctttagc
     1981 agtgagtgca ttcttccatt ggacagcagg atttgccttt agtgccggat ttgtcgatta
     2041 cttcctatct ttgaaaaatc cgattgcgaa tcagccattg atgttgatcg tccaaggatt
     2101 agtaacagca gtgatttact actttggctt taactttgct atcaaaaaat tccatctgat
     2161 gactcctggc cgcgaggaag cagatctttc agatgacgat actgcaacga ctaattcaga
     2221 caataaatat gcggcacaag caagcaaaat ctacgctgca ttaggcggag ccgacaatgt
     2281 aacttcgatc gataactgta ctacacgcct tcgtttgcaa gtcaaagata caggcctgat
     2341 cgatcaaaat aaaatcaaag ccactggcgt acctggtatg aagatcatcg acggaaaaaa
     2401 tgcgcaagtc atcgtaggta cagaagtaca attcgttgca gatgagatgg caaaactaca
     2461 tggtggtgct gcaacacgac ctgcacaaac aaacaccgtg gttaaaacag aaacatttgc
     2521 cggtggaacg atcgaacaag atatttacgc tatcgcaaat gggaaactga ttccaatcac
     2581 tgaagtttca gatgatgtat tcgcggaaaa aatgatgggt gacggctatg ctgtattacc
     2641 agaaaatgga gaaatcttct ctccaatcgc tggtacgatc acaaacattt tcccaacgaa
     2701 acatgctgtc ggaatccaaa cagatgctgg tatcgaagtc ttgcttcata tgggcatcaa
     2761 cactgttgac ttaaaaggcg aaccattcac tttatatgta aaagaaggtc aaaaagtcgc
     2821 aagaggtcaa ttgattgctt tagttgatct tgccgctatt caatctgcag gcaaaaacac
     2881 ggatatggtc gttgtattca caaatggcga caaagtccaa agcttagaaa tcgaacccgc
     2941 acgtgatgta aaagcaaatg ataaaatcgg ctctgtttcc aacaaagcct gagccatcgt
     3001 cttccccaga cttttttatc gataaacaaa caggagctga gacaaaaatc ttgtctcagc
     3061 tcctttttcc gaataaaaac ggcgttctcc tgccaactcc gcggtaataa gtccagccaa
     3121 acgtaacata tgaagagcta ttttttattt gactcttctt attacttgat gcagaacagc
     3181 ttttttacaa cctctgataa acggtgttca aaaaccagtt tatcggtatg aagtcaaatt
     3241 tatgcaaaaa tgtggaaagc cattttcaca aaatttttct tcatactcaa aactaatcgg
     3301 ctttttcaca acctctttat acattattcc tgatccaata tttcttttaa tgctgcttca
     3361 agagaagtta atggttttcc caatactttt tctaaatcat cagaggaaat agctaactga
     3421 tcctttttaa tatcatgttg gatcgctaaa aacatgtcag ctgcttcttg agggaatccg
     3481 gcttctttta gattttcgac aaacgctgca tcacttgctt caataatttc taacttttta
     3541 cctgtcgcac gctctaatgc cttggctaat tcttcatagt cagttagatt gccggataat
     3601 tctaagattt ctggagagtc actttctact agtacttttg ctgcagcttc tgcatattca
     3661 cgtttcaatg cccaacctgt ttttccattt cctgcagcat aaacgaattg accagttttt
     3721 aaagcctgcc caatgatagg taattcattc tctacatacc aattattccg taaaaatgta
     3781 tgggaaatac ctgaatcctt aatcattttt tcagtgaatt ggtgatctgg cgctaataca
     3841 ctgatcgagt gatcggcatc tgcaaaactt gtataagcga tataagatac cccagctgct
     3901 tgtgctgcct tcactacatt cttatgttcg gcttgacgat ttcctggaac gccagagaca
     3961 aaaagcaatc gatctattcc tttcaacgct tctttcattg agtctaaatc atcataatca
     4021 ccaatacgta tattaaatcc agcttctttc agtgcttctc ctttttcttc actacgtact
     4081 agtacataga tttcattttg tggaaccatc ttttttaaga attccagcgc ataaccacca
     4141 aaacctccag ttgctcctgt tactaaataa tgcatatttg ccactcccta ttctttttat
     4201 ttgttaagat ttacagctgt acatttataa attacacctt tatcataaag agtgtggtaa
     4261 gataagtcaa gcaaaaaaaa tgaaaggatg acaactgatg aagtattctc tgcaatttag
     4321 tgatgcaatc catgtacttg cctatattga aatgtttcaa gatacccgat cactttctag
     4381 tgagatgatt gccagtagta tcgaaacaaa tgcagcaaat gtacgtaaaa tcatgagtca
     4441 gctcaaaaaa tccggtttga tctttacaaa aaccggaaaa ccgcagcctt cccttgccaa
     4501 aagtccagat cagatttctt tactagatgt ctatcgcagc atcgaaggta atacacagct
     4561 tatccaagtc gatccaaaaa caaatccaaa ttgcgtcgtt ggtgcgaata tccaagaagc
     4621 tttagaagaa agctacgctc ggctgcagaa aaaggcagaa gaagaaatgg ctaagatcac
     4681 acttgatagt ctgatacata aaatagccgt tttggaaaaa gaaaaaagac cagaaaacac
     4741 aggcctcgta gagagatttt tataattctc tctactgtgc ctgcattttt ctgatcggaa
     4801 tcgtctacat aaattctttt attctccggg ggacaaaagc cttgtcatgc ccctttttcc
     4861 tctctaatgc tcaggtcaaa gcgctgaagt catgacgtct tgataaacgg tgttcaagta
     4921 gctgaccctc ggcattagtt ctgaaaaatg caaaatatgg aaagctattt cccatttttc
     4981 ctctctaatg ctcaggtcaa agcgctactt tcacgacctc tatattatca tggtttattg
     5041 actaccggta tccagatttc tcccatatat ttcccgttgg taactcccat ttcgactaca
     5101 gcatttggac ccccaacata ggcgtaatct tctacttctc ttaaaacatg cccaaaggcc
     5161 gtattcgtca aatcatttct taattcttca ggcgattctg ctaaagcgat tacaacaaga
     5221 tacgttcctt ttgggaatgg gatgattcgg gtagtgtttg gaacatcctg actcgtttga
     5281 acaccgacat agtacatcat tttcccgtta tagggttcgt tgactgtaaa gaaataatga
     5341 ttttcagcag cttgtttcaa tgcatctatt gttccattac cgataacttt ttgctcaaat
     5401 gcttgtttct ctgcagccaa accgacaaaa tccgtattgc tactcttcaa ttccacacca
     5461 atacccgtca ccgtaaaatc tggtttttct tcgatcctat actttgccat ttctcccacc
     5521 ctttctatta tattaagttc atcttacata aaattaaagg gaaatgacac gattacgctc
     5581 ttttttctag ggatattttt attttttaac tatttttttg ctttcactgc aatccaaatc
     5641 tcactgtaaa cattactgaa atcctctccc cattttgtaa aagagatctc gggagcttcg
     5701 actaactcat aatctgaaga aggaagccat tctgcataga ttttccccca agtatcttgt
     5761 aaagtttggg gaaatggccc tttattagga aaaatggccc aagtgtgttc ttctatagca
     5821 agcatttcta aatcagcgta cggattttct tttgtcgtag caaagccaat catgtgcatc
     5881 aaactgccct tttcttccaa gcgggctcca tcaaatccat aagaaacgtt gattacttga
     5941 tttggatata aatctcctaa cttatgcatc tcttcccttt gagtctccgt gatcgtctgc
     6001 gctaactttt gtatttcttg attgactcct tcaaatttga caggaacttt tttcgccaca
     6061 cctgccacat aaaactgttc tttcttttcg atacgaaatt ccatcgattt tcctcctctg
     6121 acatcaataa aaaatgtcaa gcgtggaaaa gatttttgta tgccgtatct gctgatttct
     6181 gaaggcagat atcctgacca atcacgaaaa gctcttgaaa aaccttcgac tgtttcatat
     6241 ccatatctaa acgccgtttc tgtcactctt gatccatcta ggagatcgcg attagccaaa
     6301 gccaatctac ggtttttgat atagtccatt agcgacatcc ctgcaataaa cgtaaagatg
     6361 cgttttaatt gatattcaga gattcctgta ttttcggcta ttattttata ggaaagtcct
     6421 tctgtcaaat gttcttcgat gtaatcgatc aattggttca gatctttaag catatttatc
     6481 ttcctccttg cttgcctttt tatcatatta tcaaagaaag tcaattactt gatgaatcag
     6541 gaaccaattt agtcagatgt agaaaatgtt gtatggatct aaagaaaatt ctcaagcagc
     6601 gtttcgtaac ggataattgg tattatctgc ctatctatgt tttaccttta gaaaaataaa
     6661 ttttggggaa gacacattct aagtaaacaa agcggtaaaa atatctttta ttcccttagt
     6721 taatccgctt actacctact gtacaaagca aattgttgtt caaactgctg atcagaacgc
     6781 tgcttggatt tttctttgcg atacatcata cctcttttat caattggaac aattatttat
     6841 ttctaacaaa atcacttcat ttttgtcaga taaataaaaa acaaaaaaag ttccatcttt
     6901 aaaacacgct attcttttac tttttctcac attctctaag aaaagaaaag gagaatgaat
     6961 ttttgacaat tcgagcaaaa gtgattatca aatatgtaaa atcagctact tttttcttaa
     7021 acgtttcttt atactaaata taaataagtg tttcatgaaa ggagaactag atgaataatt
     7081 ggcttgagta ttttcctgaa aacgttttgg aacgcggtta cagctatcat ctgcacggtt
     7141 ttgtccgtca tttgaactat acaagcaagt acttgtccgc aacagtttct ggcacggaag
     7201 attataaggt agtgatcact tgggatgaaa agaccaacat gacttgtgat tgtctatatg
     7261 ccatagaagg aaaaaaatgc aaacacatgg ctgctgtctt attcgcttac gaagaacgtc
     7321 caataaaaaa atccaactac tcactttccg aactctcgtc ccttgtttcc tccgctagtt
     7381 cctctcttgt acgggagctt ttaacagaga ttcttataga acatccgcaa tttatcgaac
     7441 gcttcaaagt taagatgcca tttcatgcaa taaattattc agataaatta acaactatca
     7501 tccataaata cgatcatatt atcaagaaaa acaaaaacag gaaaacagcg aagttcatca
     7561 tggagatgcg aaagttcatc caagaagcag ttgaatcgct cattcagcaa aatgcctatc
     7621 ttcccgcatt cgaattgatc aatgaagtga ttgctactct cgaaacattc tattgggagc
     7681 ctgaggatga acgaaccctt cttctgatag aggactgtta ttatttatgg aaagaattat
     7741 tagcagaagc acctcatgca gaaaaaaggc aaatgttctc ttggttcgtc tgccaagtcg
     7801 atcatactga cgcctcttat tcaaaacgct actccataaa aatcctgaaa gaagactttc
     7861 gggaaaaaga attttctaac caaaagaaaa aaatagataa aaaaactaaa gaaacggtaa
     7921 agaaagacga tttcaatgaa aaatagcaag taaacgataa caaccatcct aaatccacaa
     7981 gaaatacagc agaccttgct ttaaaaacac gaatcatgca aaggatgaag taaagaagac
     8041 ttaacgcgta cccaatatta gtcatttgac tattaggtac gcgttttgtt ttataagtca
     8101 tgacaaattt ccttcgttgc tgggtagatt ttccggcatt gttgatagat tttatgatac
     8161 ttttctacat tttgtaagag aggttccact gcatctgtat attggataaa attctgacaa
     8221 caactttcag gtgtgtcgaa ccactctaat ctttaaagat aaatattaaa ataacgatta
     8281 ataatattaa cagtctttat ttttcaccac caaaaaccta tctttgcata tactaaatag
     8341 acgtatatac acagataggt ttgtcttgtg aaaaata
//
LOCUS       DAYYCW010000113         8097 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000113 DAYYCW010000000
VERSION     DAYYCW010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.115"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACHAYA_002499"
     CDS             476..1339
                     /locus_tag="ACHAYA_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8606251.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACHAYA_002500"
     CDS             complement(1468..3090)
                     /locus_tag="ACHAYA_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8606252.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACHAYA_002501"
     CDS             3339..3860
                     /locus_tag="ACHAYA_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8606253.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHAYA_002502"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHAYA_002502"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM8606254.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACHAYA_002503"
     CDS             5941..6759
                     /locus_tag="ACHAYA_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM8606255.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACHAYA_002504"
     CDS             complement(6862..7059)
                     /locus_tag="ACHAYA_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606256.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACHAYA_002505"
     CDS             7228..7806
                     /locus_tag="ACHAYA_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8606257.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACHAYA_002506"
     CDS             7901..8038
                     /locus_tag="ACHAYA_002506"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606258.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2419 a         1472 c         1599 g         2607 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatg
//
LOCUS       DAYYCW010000114         7871 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000114 DAYYCW010000000
VERSION     DAYYCW010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.116"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..483)
                     /locus_tag="ACHAYA_002507"
     CDS             complement(55..483)
                     /locus_tag="ACHAYA_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8606259.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            575..1435
                     /locus_tag="ACHAYA_002508"
     CDS             575..1435
                     /locus_tag="ACHAYA_002508"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM8606260.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            complement(1527..1877)
                     /locus_tag="ACHAYA_002509"
     CDS             complement(1527..1877)
                     /locus_tag="ACHAYA_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM8606261.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(1892..3619)
                     /locus_tag="ACHAYA_002510"
     CDS             complement(1892..3619)
                     /locus_tag="ACHAYA_002510"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM8606262.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(3833..4759)
                     /gene="trxB"
                     /locus_tag="ACHAYA_002511"
     CDS             complement(3833..4759)
                     /gene="trxB"
                     /locus_tag="ACHAYA_002511"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM8606263.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(4904..6337)
                     /locus_tag="ACHAYA_002512"
     CDS             complement(4904..6337)
                     /locus_tag="ACHAYA_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8606264.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            complement(6337..7740)
                     /locus_tag="ACHAYA_002513"
     CDS             complement(6337..7740)
                     /locus_tag="ACHAYA_002513"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8606265.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
BASE COUNT         2159 a         1766 c         1344 g         2602 t
ORIGIN      
        1 tcaaggaaat cttgcttttt tttaaaatta tcaaatgtta ttttatttaa atccttattg
       61 acaattttca catcttccga aaatctcgaa tctatgccct tcgatcgtgc atccacctag
      121 ctgttcttcg aaataattca tcggacacat atggatttct ttcgttgttc cgcacactgt
      181 acagataaaa tgatggtgat ggccgatttt cccctgacag caatgaaaac gaaacttcat
      241 ttctccattt aaatcggttt cttccagtaa atcgatctct gcaaaatcat ggagattccg
      301 gtaaatcgta tcataactta cccctttgaa ttcctgattc atatagtcaa aaacttcttt
      361 agcagatacg tagcggttac gcttcactaa ataagttaac aatgcttctc tttttttagt
      421 atatttaaaa ccgttatttt tcaatgtttc taatgattgt tcgatcaatg tctgtttttt
      481 catgacttat tcctccaaat caaaatgatt acgaagtacg cattctagag tatggtataa
      541 taaacataaa aaaaatgcaa gtgaggtttt atccatgtct tccgattcta cagaaatcgt
      601 tacttttttt gtcatctccg actctgctgg agaaactgcg acaaaactag cgcaagctac
      661 tatggctcag tatcctaccg ttgagttcaa cctattccgc agaacgttcg tgacagatga
      721 aaaaacatta aggcaggcat tgcaagatgc attagaagaa aaagcacttg tcctgcatac
      781 attaatcaat caagaactga tcgagataac acgttctttt tgtgaagaac atcaattatt
      841 tagttttgat gtgatgacgc caccgatatc agaaattgaa cgtttaactg gtatcaagcc
      901 aattcggcaa cctggtgccc tccatttatt gaatgagaac tatttcaaac ggatcaaagc
      961 aatggaattt gctgtaaaat acgatgatgg gaaagatcct cgtggttttt tagaagcaga
     1021 tgtcgttttg ctaggcgtat cccgaacttc caaaacgcct ttgagcttgt ttttagctaa
     1081 caaaaattta aaagttgcca atttaccttt gatcccacaa gcacatattc ccaaacagct
     1141 ttgggagatc gatcctaaaa aaatcgtggg actaacgaat aacccagata ttttaaataa
     1201 tatccgtaaa gaacggatgc gctcttacgg attaaaccct gatactgctt attccgatat
     1261 cgaaaaaatt agagctgaac ttgattttgc gaatgatctt tatgaaaaac taggatgtgt
     1321 agtgatcaat gtggcatctt tatctattga agaaacggct tcgcttattt tgaatgcttt
     1381 agacctagaa gatcatagtt attatggaac tgaaacaaca gaaaaaagcg attaatcatt
     1441 tttgtctttt tcttaaaaaa cactggagtg tgacaacgaa gaaatttcgt tgtcacactc
     1501 cagtgtttat ctatttgcac ctatcgttat tgatgttcca catgtttgag tgtctggttc
     1561 aaaatatcca gtacatgaga atcatcaaga ctatacaaaa tcgcttttcc ctcgcgatga
     1621 gacttgacga catgattttc tcttaataaa cgtagttgat gcgatacagc agactgctcc
     1681 atttccactg cttgggtgat tgctgtcaca ttttgttccc cgtctttcag atatagcaga
     1741 attttaagac gagtggggtc actcaacatt ttgaataatt gactgacttt ctctactttt
     1801 gatttttgcg tgtctggcaa tccattcacc tccaacttga aaaaagactg acttctaaag
     1861 tttaccactt tttccatcag tctcaagttt tttattcttc tgtcatcttt ttgataatcg
     1921 cttcataaga ggctactttt tcggctgcgt cttctgtaga gcttcctttt acaccaatat
     1981 aaaacttgat tttcggctca gttcctgaag ggcggatagc gatccagcca ttttcttcca
     2041 atgtatactt caatacgtcg gatggcggtg ttgttagttt ttctacgttt ccttcacggt
     2101 ctgttctcgt caattcttta aagtcttctg tttgaataac ttttgctccg gcaaattctg
     2161 tcggtgcttc tttacggaat ttttccatca aagcagagat tttcgcagaa ccttcttgtc
     2221 cgctcattgt cacagaaatc gttttttctg cgaaatagcc ataggtttca aaaatcgttt
     2281 gcaagccgtc atacaacgtt ttgccttgtt ttttatagta agctgctact tctgctaata
     2341 aaagcaatgc ttggatcgcg tctttatcgc gaacaaaagg tttaactaaa taaccataac
     2401 tttcttcgaa tccaaacata aaagtatgtg aatggtcttc ttcaaattgt tggatttttt
     2461 cagcaatgaa tttaaaccct gtcaacacat tgaacatagt agcaccgtag tgttccgcaa
     2521 ttgctgtagg caattcgctg gaaacgattg atttcaatac cgctgcgttt tctggtaaag
     2581 tgcctcttga ttgatgtgct tccaaaatat attgagcaaa caatgctccg atttggttcc
     2641 cagtcaatac ttcataagaa ccatctggca aacgaaccgc tgcgcctaaa cggtctgcat
     2701 caggatctgt tgcaatcagc aagtcagctc cctctttttc tccaagtcgt atagcatatt
     2761 caaaagctga atgttcttct ggatttggcg atttgaccgt agtgaaatct ggatctgcca
     2821 cagcttgttc gggaactagt acgaattgtt cgaatccagc ttgtttcaac gcacgttcgc
     2881 ccaacatttt ccctgttcca tgaagtggcg tgtacacaag tttcaaatct ttccccattg
     2941 tttcaaccaa ttcacgatca atcgtcactg ctttgatttc ttctagataa gcagcgtcca
     3001 cttcttctcc gatgatcgtg atcaacccgc tatgtttagc ttcttcgtca gataatactt
     3061 cgacttttag cggattgctg acttcacgga catatttcgt caatgcatct gcgtctgcag
     3121 gaggcatttg tccgccatct tctccgtaga ctttatatcc attgtaagcc gctgggttgt
     3181 gagatgcggt taccatgatt cccgcaaacg tcttcaaatg acgaactgca aaagaaagct
     3241 ctggtgttgg acgcagactt tcgaatacat aagacgggat attgtgatgt gccagtgttt
     3301 tggctgcttc cattgcaaat tctggtgact gatggcgtga gtcataagcg atagcgacac
     3361 ctcggcgttt tgtttcttca ccttgtgtat ccatgaaacg tgctagtcct tctgttgctt
     3421 gtcttaccgt atagatgttc attcgattga tcccaggtcc taagattccc cgcatgcctg
     3481 cagtaccgaa ttccaacggt gcataaaaag catcttcaag tttttcagga tcttcaccca
     3541 gatcatttaa ttgttttttt aagttttctt ctaaattttc ttcatgtacc cattgttcat
     3601 acacttgttc ccaagacatg aaaataacac ctctttctga aatatggttc ttaataagta
     3661 tagcattcac ttattagata tgaaagtatg aggaccaaaa aacgcttaaa tcaagaaagg
     3721 attcttttat caagcatgat tgcaagtatt cacataaatc ggattcagtg gatttcacgc
     3781 gaaaaaaaag agtaaaagaa caattgaaac cgttctttca ctctttttaa acttatgctg
     3841 ttgtttcttc taaagactcc aagtatttat acacttgttg tccagcaata ccgccgtcac
     3901 caacagctgt agtgatttgt cgcaaatctt tttcacgaac atctccgatg gcaaaaacgc
     3961 ctggcatatt cgttttcatt tcgttgtctg ttggaatcca gccagcttcg ttcgtcaaac
     4021 ctgcttctct aaaaggtgca gttaatggat ccagtccaac atagatgaat actccgtctg
     4081 ctgctaaatc aaattcattg ccggttttga cattacgtgc tttcacacct gtcactacca
     4141 tttcgtttcc aacgatttcg tcaacgactg tatcccacat aaaggaaatt ttttcatttg
     4201 caaatgcacg gtcttggatg atcttttgtg cacgtaattc atctctacgg tggacgatca
     4261 ctacttcaga agcaaattgt gtcaaataga tggcttcttc tactgctgag tccccacccc
     4321 cgatgactac tagacgtttg ttacggaaaa acgcaccatc acagactgca cagtaagaaa
     4381 cgcctcgtcc agcaaattct tcctcgccag gtacgcctaa tttacggtgg atacagcctg
     4441 ttgcgataat gactgttttt ccttcgtaac ttttatcttc acagatgact tctttatggc
     4501 tgccgtgatc cttgatatct tgaacgattc cataagcatt ttctgtccca aatcttgaga
     4561 cactttcata catcttataa gctagatcgg ggcccatgat cgaatcgaat ccaggataat
     4621 tttcgatttc tgccgtattg ttcatttggc cgccaggggc accttgttcg atcatcaata
     4681 cggataagtt tgaacgtgaa gcatacaaag cagcagtcat cccagcagga cctgcgccaa
     4741 taacgattac atcatacata tgtagattct cctttactta atcaagtaag catactttac
     4801 agccaatgcg aaaaaaagtc tattgttctg cttgcgtttc tcgaatagct ttattatact
     4861 cgaaaaatcc gctgaaaaac aatagacttt tataaatcat tgatcaattg ttcgaaagat
     4921 tttctccatt tgaatcaatt acttttttat accagttgaa tgattttttc tttgaacggg
     4981 ctaatgtgcc atttccttgg ttatcacgat cgacataaat gaatccgtac cttttcttca
     5041 tctcaccggt accagctgat actaaatcga tacatcccca agtcgtatag cctaatagat
     5101 cgactccgtc tagttctact gcatccttca tcgcctggat atgcgctgcc aggtaatcga
     5161 ttcggtaatc atcttccaca taaccatttt catctggcgt atcgactgca cctaagccgt
     5221 tttctacaat aaataatggt ttttgataac gatcataaat atcattcatc gttattctca
     5281 agcctaatgg atcaatttgc cacccccatt cacttgcttg cagatatgga tttttgacag
     5341 aagcaaagat atttcctgca gtctgttcca agagttcgtt gtcggtagtc gaaactcgtg
     5401 aagaataata agaaaaagag ataaaatcaa cagtatgttc tttcagcaat gcggcatccc
     5461 ctggttccat ttcgatctga atgccatttc gctccatttc tttcaatgca taagcgggat
     5521 attctcctcg tgactggaca tcaatgaaga aataattttc tcgatctgat tttctagaag
     5581 cccaaacatc ttcaggattg caagtgtatg gataatatgc tcctgctgcc agcatacagc
     5641 ctactttgtt ctccggatct acttcatgag caatcttcgt tgcgatggca cttgcaacca
     5701 actcatgatg tgccgcttga tatttgattt gttcttcatt ttctccttct tcaaaataca
     5761 gccctgcgcc cataaatggt gcatgaagga tcatattgat ctcattgaat gtcagccaat
     5821 atttgacaag tcctttatag cgattgaaga tgactcgaca cagattttca taaaaaccaa
     5881 cgagttctct ggaacgccac gcaccatatt ctttgatcaa atgcattgga caatcaaaat
     5941 gcgtaatcgt cacaagtggc tcaattccgt gcttttggca ttctttaaag acatcctcat
     6001 aaaatttcaa gccagcttca ttcggctctt tctcatcccc cattggaaaa atacgactcc
     6061 aggcgataga cattcgataa gtcttgaacc ccatttctgc aaacaaagca atatcttctt
     6121 tataacgatg atacatatcg attgcaattt ttgctggata aaaatgatcc tcatcaaatt
     6181 caaacatttt tttctttccg gtgataacag gaaaacggtc ttttccgatt ggcacaacat
     6241 ctacgtttgc tagtccgcgt ccaccttcat cataagcgcc ctcacattga ttggctgctg
     6301 tagcaccgcc ccataaaaaa tcttttctga accccattat acttcctcca ttatatgtgt
     6361 attacctgat aattctcttg ccagttcatc ttgaacttcg attccttctt cttttaatcg
     6421 gctgattgtt tccttgaatt gcggtaaata ctgctggtgt gcaataaaca actcatccat
     6481 gatccgtttt gctgtctttc ctgatcgtat aagtggattg atcgtaaacg cctgtaaagc
     6541 tgtcccataa tcaccagtca cagctgcttc gatcgtcagc aattccatat tcttcattac
     6601 ttgcaaccag cctctttcag cagcaggaag agaaccaaac gctacatttc tcgctccttg
     6661 tgcgccaaca taggcagtga cttctacaac acaatctgcc gacaggtcag gaacagctcc
     6721 atcattcttt gtagatacaa cgatctgtgt ttctttattt ccatagattg aagcaatcgt
     6781 ttcacaggca gcgtctgaat aataagcgcc acctcgtttt tgcaattgtt caggtttatg
     6841 gtctaattct ggatctttgt ataattcaaa tagctctgct tctgtttctt tcacttgctg
     6901 cgctcttgta ccaatcgttt gatattcttc taaagcatgt tccagcattt cttcttcacg
     6961 atagtaatag cggtggtagc cacaagggat catgttcatt tgttttaatt gttctttgaa
     7021 aaacgggata tcaaagatat ttttcggtaa tccattatct tcatcgtata aacgatcaat
     7081 caattctgcc gtgcggtcat ttcctttatt atctgctact ttgtgccaat ggaaatgatt
     7141 tagaccggca aacttgtaga tcagctcttc ttttttcttg ccaagcattg ctggttctgt
     7201 catcatcgcc atcactggca cattgcataa tccaatcact ttatcccatt tgccatatct
     7261 gataactgct tctgtcacca tcccacttgg gttcgcgaaa ttgatcagcc aagcatttgg
     7321 acataaacgc ttcatgtcct caacgattgc caaaacgact ggaacggtac gaaaagcttt
     7381 gaatatcccg ccagcaccat ttgtttcttg cccaatcatc ccataataac tagggatccg
     7441 ctcgtctttg attcgtgcat ctaattgacc gacacgaaac tgagtcgtaa caaaatccgc
     7501 atctttcaat gcttcttccc gatccagagt tgcatagatc ttgacttcat atggagaagc
     7561 atcccacata cgctgagaca tctgactaac gatctcaagc ttttcttttc cttcttctac
     7621 atctacgagc cagatttctc gtatcggtaa ttcctcatat cttttgataa atccttccat
     7681 caactcaggg gtgtaactgc tgcctccacc aatcgtagcg attttgattc cttttgccat
     7741 gttctcactc ctttactttt ttacactttc attatctttt ttgtaaacaa aacaaacaat
     7801 agaaatccgt ttctttccag taacatttac aaaagttact gttttctagt ggatttttgt
     7861 aaatatcttg t
//
LOCUS       DAYYCW010000115         7837 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000115 DAYYCW010000000
VERSION     DAYYCW010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.117"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..813
                     /locus_tag="ACHAYA_002514"
     CDS             <1..813
                     /locus_tag="ACHAYA_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFM8606266.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            786..1229
                     /locus_tag="ACHAYA_002515"
     CDS             786..1229
                     /locus_tag="ACHAYA_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606267.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2399..3367
                     /locus_tag="ACHAYA_002516"
     CDS             2399..3367
                     /locus_tag="ACHAYA_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8606268.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3384..3908
                     /locus_tag="ACHAYA_002517"
     CDS             3384..3908
                     /locus_tag="ACHAYA_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8606269.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            complement(4079..5227)
                     /gene="tnpB"
                     /locus_tag="ACHAYA_002518"
     CDS             complement(4079..5227)
                     /gene="tnpB"
                     /locus_tag="ACHAYA_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFM8606270.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(5244..5648)
                     /gene="tnpA"
                     /locus_tag="ACHAYA_002519"
     CDS             complement(5244..5648)
                     /gene="tnpA"
                     /locus_tag="ACHAYA_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM8606271.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            6175..6456
                     /locus_tag="ACHAYA_002520"
     CDS             6175..6456
                     /locus_tag="ACHAYA_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606272.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            6458..6874
                     /locus_tag="ACHAYA_002521"
     CDS             6458..6874
                     /locus_tag="ACHAYA_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606273.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            7496..>7837
                     /locus_tag="ACHAYA_002522"
     CDS             7496..>7837
                     /locus_tag="ACHAYA_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HFM8606274.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2814 a         1358 c         1268 g         2397 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaaac
     1441 actttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
     1501 ccgtaggacg tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca
     1561 aaaaaagtta aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac
     1681 ttttaaaaaa gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga
     1741 cgtttgaaac gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa
     1801 ttttagaggg cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga
     2041 ttcccagttg agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctata tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg
     2341 cattttgtat ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat
     2401 gtctaatgaa atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac
     2461 accagtcgaa atgaatttgt tcttttctat cgtatcaaga atgcgtgatg taggtgataa
     2521 aaaagtacaa tttacattcg agcaattaaa agatttaagc aattataaag caactgcaaa
     2581 tgttcgattc atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg
     2641 tagaagaagt gctgacggtt tgcaacgtga acgttttgtt ttatttaatc aatttaaaat
     2701 cgacggaaaa tctgatattc cttttgctga aatacaagtt catgaaaaag ccttaccctt
     2761 acttaataat cttgaagaat gggtgcgcta ttctctccaa caattcaatg aattagaaag
     2821 cagttattca aaaacaatgt ttcgattact aaaacaatac agaaccaaag gtttcgctta
     2881 tttttcaaaa gaagactttc atgaattact tgatattcct aaaagttaca accaaggtac
     2941 gattgatcaa cgtgtgttca atccaatacg acaagaacta accgcaatct ttaaaggttt
     3001 aacaatcaaa aagaaatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac
     3061 atttaaacct gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga
     3121 acaaaacaaa gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc
     3181 attacaagaa aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa
     3241 gttagaaaga gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa
     3301 ttccgatatt ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa
     3361 agattgaggg gtttttagat tttatgacag aaaaaacaat caaagaactt gctgaagaat
     3421 taggagttag taaaacagca attaacaaaa aagtttctga cgaaaatcga aaactttggt
     3481 tttcgaaaat cggaaacaag tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa
     3541 tgttttttaa cgaaaacgaa aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg
     3601 aaaaccacaa aaacgccaac caagttcccg aattagaagt cctttctctc ctagaacaac
     3661 agttaaatca aaaagatatg caactacaag aaaaagacga gcaaataaaa caaatgcaaa
     3721 aattactcga ccaccaacaa caactcaccc tgcaagccaa ccaacaaatc gagaaactgc
     3781 aggaacaact acagttgact tatacggaag aatcttcgga aaataaatca acgactcttt
     3841 ctgaagaaac agaatcgata gaaaaacaat caaaggcgga gaaaaaatgg tggcaattct
     3901 ggcaataaaa ccagcaatgt gaactactca ccacttagct aaacctgacg gttcttaacg
     3961 cttgaagtgg gagcttcttg ggaatagagc atacttgata gcgtatttct ttactaacag
     4021 cggtgtctct tatttaccaa gctatccccg tagttcctac ggttcttgat gattggatct
     4081 aagccaatcc catagaatgt agtcttagac cttcggtcag aatattgata ctagcattga
     4141 tatcacgatc atgatgagta tgacaaacag gacaagtcca ttctcgaatt tcaagagatt
     4201 tcttgccatc tttatgtccg cactctgaac aaatctgact agacggaaac catttatcta
     4261 ctttgatgat ttttcgtcca taccagtcag ccttgtattg taatttagtc acaaactttg
     4321 accaagaaac atcagaaata cttttagcta atttatgatt tcgcaacata ccttttgtgt
     4381 ttaagtcttc aatacagata atatcgtgat ttttgatcat ttctgtactc aacttattca
     4441 gaaaatcagt acgttgattc attacttttt catgcaatct agctactttg cgtttttgtt
     4501 tttgataatt tttagcttca aatagattgg tacctttatt tttagccaat aaagcacgcc
     4561 tagacaattt gcgttgttca cgctttagtt tcttttccat tttggacgtg aatttattat
     4621 tatcaatttt ttgtccatca gaaagaatcg caaagtccat aatacctaag tcaataccaa
     4681 ttgtggaatt agttttaggt aattcgataa tttcttcttt acacaacaaa gaaatatagt
     4741 atttaccgct agaatgacgt gatattgtag cggatttgat aatccctttt ggttgtctat
     4801 gaagcttaat tcttactaat gacttcaatt taggaacttt gatgaattta ctatcaatca
     4861 aagcaactgt accattttga ttattcgttg tataactctg aacaggattt ttcttacttt
     4921 tgaaacgtgg aaatccaacg gatttatccc gaaaaaaatt cttgtatgct ttatctaaat
     4981 taagttgggc attggctaaa gcaaggctat caatttcttt caaaaacgga aattctttct
     5041 tgtatttagc tggtgtcgga aaagtcattt ttttagaaga atcattttta acttcttcat
     5101 acgccttttt tcggtcatca agcattagat tgtagacctt acggacacaa ccaaaagatt
     5161 tggcaaagaa gatttcttgt tcctctgttg gatagattct gaatttgtat gcttttagtt
     5221 gttccataaa gttcacctac tttctattta tgattgtttc cttgattttg gatatacttc
     5281 ttaatcacat caattggtgc gccaccagtt gtcagaagac aaaaactttt agaccagaac
     5341 atttctttcc aaagaaattg tttgactctt ggaaagtcac gttttatcaa tcttgagctg
     5401 gcacttttat aggcattgat gaattttgtc aattctgttt taggttgagc tttgaacata
     5461 atatgaacgt ggtctttatc atgattccac tccactaaag taatgtgata cgattctgaa
     5521 attctttcaa aagtagtttt ggcataatca gatatttcat catcaatcac ttgtctacga
     5581 tatttcgtta ctaaaacaag gtggtaataa agaagaaata ctgaatgatt atttgtatct
     5641 aattccatgt tatatcaact catttcttgt atagactgat tgtaacatgg aacaaaataa
     5701 aaaggcaata cgccttagtt gtcggtcttc gagttaccaa cggtaaccct cataccgaac
     5761 ggcattcatc acccacctat agaggatggg cgacttctgc ctaattctgt taaattaata
     5821 aaaattgctg gttttattct tctttttgct tttgcgagca gagcaacatg gacccatgtt
     5881 gcgatatttt ggtggcgccc caatccccgt tttttcgttg aatcttactc ggactgcact
     5941 cgttttacag ctgatttttc tgaattttga aagagcataa ggaaacgtgg tatcactttc
     6001 ttacgaaagg tgctagatag gctgtgccta tcacgtttct cagaaaaaaa tttgctatca
     6061 cttttttgcc attgtggcta ggctatgtat agtgttttgg cgaaaaatag ctatacaaat
     6121 actatatttg tatggtatac ataaattata cgctgatacg aaaggaaaat ttccatggac
     6181 attttagttc gaaatatcaa tcccaaaatt attgcaagaa tagataaacg ttgtcaggaa
     6241 ctcacaacca aaacgggcaa aaaatggagt cgaaataaat atctaaaagt tctaatcgaa
     6301 agtgattttg accgtcaact tatgaattac aaaaaagatc aattcgattt attactcgaa
     6361 aaatttattg ccattcaaac agaaaatacg aacatactga atgactatgt tcacacaaac
     6421 aaccaactca ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa
     6481 acacgtaacc ctttatccca gtcacgttga cgttttagat tccatcatcg aaaaaaatcc
     6541 agacgtcaac aattattcag aagccattcg ttttttatgt atgagttatg aacctgatac
     6601 caatacaacc acaaccagta accaagcaaa actcaatgcc atgggaaaag aaatttctat
     6661 catgacagaa atcatggcag aatttgctga tttatatcta aaagatactg gtgttcttat
     6721 cggtacaaat tctaaagtgt atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc
     6781 ttcccgacga aatcaaccca aaaatcgtca aaaagaaacc aatcaaacct ttacttctgg
     6841 aaattttgac ccaacaaact ttctaaaaag ataaaaaaag aaaccgatcc ctaaaaaaat
     6901 tcctcgattt ctttttaggg atcagtcttc cagcaagatt tgcaagttgg taccaacttg
     6961 cgaaaaaaaa gaaaaatccg cttatgcaac ttcttctttt tgtattttga ggcgctaccc
     7021 caatccccaa gtatcggtta tttaatagcg cggaaatcca aagaatagga cgtaccctga
     7081 aacaaaaaaa gactgctctc acgagcagcc caaaatgaga ttatcctatt catcaacata
     7141 aaaatattac aaagtgaaga actagagaaa gacaaaaaaa gcatatcaaa tcctctcaaa
     7201 aataacaagt ctttaaaaaa tgacagataa aaatgcacaa agaactaaaa aagagaaatc
     7261 gcgtaaaatc aagcattaga agctagtaac tagctagacc aattaaaaat cacaaagtga
     7321 gcgtctctat ccattggtag aggggctact tggcatagtc gacattatgt taactaacgc
     7381 tataaataac gattatgttc ccaaaacaat attttatgaa ctataacaaa aatttgttat
     7441 cgcatattgt atattttaat tataggtgta taccacttaa atttaacaca aaggcgtgat
     7501 tattttgaaa cccaaaaaga aaatttatct aggtctcctc ttttccttga tgattggaat
     7561 ttttagtata ggaattacaa atacctatgc ttcagaaaat tctaatttca atatcaactc
     7621 ccaaccaatt gaagaacaag caattaattt tgcgaaaact gattttgaac actccataac
     7681 ttcaattctt ttaatggaaa ataacaccga aactgatatt aactatactc ttggttcgcc
     7741 atttaaaatg ctcgatggag aaaatacggc aaatagttct attttctttc cagtattttc
     7801 agacgctgat aatacgatta aatatattta taccgtc
//
LOCUS       DAYYCW010000116         7812 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000116 DAYYCW010000000
VERSION     DAYYCW010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.118"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..1384)
                     /gene="celB"
                     /locus_tag="ACHAYA_002523"
     CDS             complement(32..1384)
                     /gene="celB"
                     /locus_tag="ACHAYA_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8606275.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1384..1902)
                     /locus_tag="ACHAYA_002524"
     CDS             complement(1384..1902)
                     /locus_tag="ACHAYA_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606276.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1918..2244)
                     /locus_tag="ACHAYA_002525"
     CDS             complement(1918..2244)
                     /locus_tag="ACHAYA_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM8606277.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2257..4236)
                     /locus_tag="ACHAYA_002526"
     CDS             complement(2257..4236)
                     /locus_tag="ACHAYA_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8606278.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4257..4571)
                     /locus_tag="ACHAYA_002527"
     CDS             complement(4257..4571)
                     /locus_tag="ACHAYA_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM8606279.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4739..5032
                     /locus_tag="ACHAYA_002528"
     CDS             4739..5032
                     /locus_tag="ACHAYA_002528"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM8606280.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5110..6222)
                     /locus_tag="ACHAYA_002529"
     CDS             complement(5110..6222)
                     /locus_tag="ACHAYA_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8606281.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6375..7223
                     /locus_tag="ACHAYA_002530"
                     /pseudo
     CDS             6375..7223
                     /locus_tag="ACHAYA_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7576..>7812)
                     /locus_tag="ACHAYA_002531"
     CDS             complement(7576..>7812)
                     /locus_tag="ACHAYA_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8606282.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         2495 a         1554 c         1262 g         2501 t
ORIGIN      
        1 ttttttgtca cagttccttt tttcttagaa atcattttaa cttttcacta gctaattctg
       61 ctgccttttc tctttcttca tttaataatt tattatcata gaatttgata aatgggaacc
      121 agataatgaa tgcgatgatt gcacaaatga ctgctaacac agcagctttc caatcaccac
      181 cactaccaac aaatgctccg atcccgactg gtgaaggcca cggcatctgt gcaagcatcg
      241 gtttgacgat ttccagccga atggcaaaat aagcgagtgt agcagaagcc atcggagcta
      301 agaaaaacgg aaccgctaaa tagggattgt agacgattgg catcccgaaa atgattggtt
      361 cattgatatt gaataatcct ggtacaagtg aggcttttcc taacactttc agctgatcag
      421 atttcgcgag gaatgcaata aagatgatca atcctaaagt cgcacctgat ccaccaacgg
      481 ttacataaga attgttgaat tctcctgcca acggataatt tgcgccttgt gcgttagctg
      541 ccatattagc taatacgatc ggggtcaaga aagaagtgat gatattcgct ccatggatcc
      601 ctacgatcca caatgcatgg atcaagaaat aaatcaccat gacaccaagc catgtattcg
      661 tcaactgcgt aacaaaccca aatgggatag caatcatttt gaagatatcc gttcctagtg
      721 caactaataa tccattgatg atcatcacca caaacgcaat gacaaatgtt gggatcagag
      781 cagtgaaaga acgagaaact cctggcggaa caacatctgg catttttatg acccaatttc
      841 tcttgacaca catacaatac aattcgaccg caataatcga cataataata gctgtaaaga
      901 ttcctgaagt tcctaaacgt tcgacaaaag agcccatcct aataccattt actacagttt
      961 ctgtctcatt gatagaagca atcaagctga ttttaccatc agtcatgatc aattcaggga
     1021 tacacataaa gaatgcaaaa acagatagta atgcccccgt gagcggatta acatttaatt
     1081 tttgttcatc tcgttcaatt gacgttaatt cataagcaaa cacaatggca aagtaaatag
     1141 ataaaatccc catcgtcatt gtattgacga tcatgtacaa gtcactaact ttgaaaaaag
     1201 ttgcattaaa aaatccttct aaaaatggga acggtaatgg caaaatattt aaaaccaaaa
     1261 acatcgagcc tacaatcgtg aaaggaatag aagccatccc cgctgccatg acagcccgaa
     1321 cgattttaag ttgcgcaatt ttccccatag gtcccatcag gtatttctgc aaaaaatcaa
     1381 acattagtta ttctcctttc ctaaatataa cgctttttca tattgtttaa tacggatata
     1441 ctgttggtct tggttgagtg aaattttctt taaacgatac tgtataaatg cactggcaaa
     1501 aataccgctt acaacaagaa tgaaaacaaa taactgtcct ccattgttat ttgccataaa
     1561 aggatacaag aatgtaaatc ctgaggtaaa caacactgga atcactaata aattaaagac
     1621 actctggaac aaaaaatacc actttgtcca tggaacgttc gtttggtgtt ttcgataaag
     1681 ctttacttgt tctcctactg caggcaaaat agctaaaatc agaatcatcg gtaaactact
     1741 tcctaaactt ttagctgata gaagcagaat catccattgc atgttcatga aaaagaaaaa
     1801 ggcagtcagg taacgtacga ttagataacg attgaaatac atgtttttca aactaagttg
     1861 ctttttttct aattcgattt tcctttttac tgtatcctgc acaatgatcc ttcctttcta
     1921 tttatttttt tgatacaaat aattcatttc ctgtgcaact tccaataatg tcatcgtcgt
     1981 catcagatga tcttgtgcat ggaccatgat aatttccatt tctatttttg tcccgctggc
     2041 atattctttc agaagttgag tttgtgactg atgcgcatga agaatctctt catttgcttg
     2101 tcgcaacttc tcttctactt cttcaaacat ctctttacgc atcgagcgaa atgcttcatg
     2161 tatcagtgtc cgtgcatttc cgctatggaa aatgatatca aaagaagtca cttgtagtgt
     2221 ttcactgttt attttttcag ccatctgttt tcctccttat tcgatcagtt tgataaattt
     2281 actgctgaaa ttctcaaaaa taggttcagc taagatttcc tgctgtactt ttagctggtc
     2341 aactaaccta acgattgctt tagtcacgac cgtaattcct tcattttcga cataagaagg
     2401 cgaaactaaa aaaacaaaat gaatttcttg atattgatcc cactgcatcc cagaaggaat
     2461 caatgcaacg gctacctttg tttcgattcc aactggaaca gcgggatgtg ggactgccac
     2521 tgtatcactg aatatgattt gtcccatatt ttcacgttgc tggatctgct ttcgcatttc
     2581 tcttatatat ccttcttctt catttatgct cagcttttgc agcaaatcat gaagaatttc
     2641 ttcctttttc ggttcgtacg tataagtctt gaaaaatttt tcagacaact gttcggtgta
     2701 aatctgcttt ttttgctgta gagaaaatgc tggtatcggt ctatctgagg aatgataggg
     2761 tatagattct ccaactacct ttcgtatttt acgaacatca tcttcattca aaaaaatact
     2821 gacatgtaaa actggaattt tgaaaaacat tgtcgaaaga tcaatagaag aaataatcag
     2881 gtcgattcct tttaaagttg tttcattgat ctcataatag ccttttacac ttaccactgt
     2941 aatattttta ccgaattcac tgactacgcg attctttaat agttgggcac tgccgtaacc
     3001 cgttgcacag ataatcaatg catgtagttt atgcgcagct ctttcttttt ccaatgccgc
     3061 catgagatgc aaagccaaat aggcccattc atcttcattg atggtatagt ttttcaaagc
     3121 cggcatgttg ctgaaatgcc tttttactgc gtcaaagact ttgggatttt ctttctttat
     3181 ctcctcagtc aatgggttct caatgcgaat acctcggctc aatcgaatca tcatcggtga
     3241 caaatgatcg agtagtccat tgaaaagctg gagatcctct actaatgaaa aatgaaattc
     3301 ctgagacaat cttttcagaa ctttcgctaa ttcctttgat agttcctgat cattttgatt
     3361 ttctttgtga ttcgacttag ccatgagatg taatgtcaaa taagcaattt cttcttcggg
     3421 aaacgataca cctgcgatta cctcaatacg ttgaacgatc cgctgcgcca cttgatactc
     3481 tactcgctta gaaacttctt gttcgatgcc taattccttg atttcaaacc cttctcggag
     3541 ccgtttgatc cccaaagcca agtgtaaagt cagattctgt atgataaaat ccgaaacttt
     3601 taacttagct tctcttgttt catcgagaat gataatgact agctcttcaa aactgatgtc
     3661 tgggaaaaaa cggctttgcc ctaaatattc tttcaatgaa tttccataat ggtttccaaa
     3721 aaaagtatcc atgataaaat gccgtcgatt tctttcttcc ccttcgatcc atgtcccttg
     3781 accatgctta gaaacgattt ttagtgaata tggaatcaat cgctccttga tttcttgcat
     3841 gtcctttgtc aaactggaac gactgatata aagctcttct gccaaatcat ccataaaaag
     3901 aatggcatct tccaaaagca attggttcaa tatatacttt tgtcgatctt cggactcata
     3961 aaatccttgc ggttctctag catatccagg tactatttct tgccgactca aaaacaagtc
     4021 aaatgtcagt ctttgtaaaa tatgcagttg gtagccataa cctggtttag caatgatctt
     4081 acctccgttt ttttctacta actctttgat ccgctgcaag taattacgga ttgtccgatc
     4141 agataataac aattcagaag ctaaacgttg actggtgaca tactccttct tgtgatcaat
     4201 caaatatctc aacagcaatt tttccttctg tttcattatc atccactcct attccatcat
     4261 atgttctctt cgatcacctc tgctaacttc tggatgcctg tagggattgg gacatacgct
     4321 tggaaaggga tactaacaac aggctttcca acagattctc ctaatttttt gaatttatcc
     4381 aaaaacatcg tcgtttgcgg gctgatcaga aataaatcaa aatcactatt cttaatcata
     4441 ttatcgcctt ctgtagctga aactgcatct acgagtacct cttctctttt ttgcttaaag
     4501 aaatccgttg tctttgaggc catcatcgag gatgacattc ctgccgcaca aataatcaat
     4561 gcttttttca taaatcaatc actcctttta tttttataaa ccaagcatat cttgtaagcg
     4621 ttttctattc aaaatatata tttccgtagc attgcggaaa aaatgtgtat catatcttga
     4681 ataataattt ctggcaccgt gatatactag tgatagaatt ttttcaggag gggatttagt
     4741 gagtatcaca gtgaatattt tgtatacaag tacaaatgga caagccagaa aatttgcaga
     4801 agaaatggtc gaaagaaaca ttgtcaaagc gattcgtgca gaagaaggaa acgagaaata
     4861 tgactattac tttccactag aagatccaga atctctttta ctgatcgacc gttggaaaga
     4921 tgaagaagca atcgaaaagc atcacaaatc cgaaatgatg gctcaaataa acgaactccg
     4981 taaaaagtat aaattaaaaa tgaaagtcga acgtttcact gatttaccgt aaaaagatac
     5041 acatggaatg atagcaattt atcatccatg tgtatcttca ctttttcctt attttttatt
     5101 cgatctcatc taatgattca aaccgtgttt ttctcttatt tttcggataa gtatttcagt
     5161 caccccaata tacatgattc ctaaacatac cccaaggata ttctcaaaaa tgcggatcgt
     5221 tactgctcct ggaaccccga ataaactggc agtgattgcc aaagcaccaa aagaattaaa
     5281 aactgtttta tatctatagg tagaacaaac acctaaagct agaccaccta atataccgac
     5341 ccagttaaaa ggaataaatt ggctgatacc aataaataaa acacatccaa tcaatgagcc
     5401 aatgattcgg tctactgctc gttctttgaa accaatcgac atcaatccat agctagatac
     5461 aatagaagaa aatgcaaacc ctgcccacat caatcgctga aaagggacat actctcctgc
     5521 aacgatcaaa agactaatgc caaatgcata gatgagcatc caaatttttt cctgcgaata
     5581 catgcctttc aaaaaaatct ctttaaacaa gcttttgtct ttgttctttt ctcgatgttt
     5641 tctgtaaagg agaactgaaa accaacaaaa aaacaaaaat aataagctac ttgtttgtgt
     5701 aaagaaattc ttgttcaacg aatctttagg taacgaataa acaataaaga gatatgaaaa
     5761 gccgtacaaa gaagcattcc ccattttggg atcactagcg gttgccatta atatgctgct
     5821 tagtaaaata aaatgaatca acatgcccat aactgaccat ttgatcagct ggacatacgg
     5881 tgctactaat agtgagaaca tgacgattgc taaacctatg atcgaatggg aaatcttata
     5941 accaaagtca acaaaacgaa tactcatcaa catacagaat aaaaccacag cgtatggttt
     6001 atgcttcgat tcaaataatg ttgtaattat aatgataaaa caaatggcaa acaatacgat
     6061 gaataatgat cgtaaaaaca atgaacaaat atacatgcac ttttcttttt tagaagctgc
     6121 ttctcgtatt ttttgtttta aaataaacgg atctagttgt agagactgat aaaaatccac
     6181 ctgttgtcgt cctttctttt tacttttatt ttttacagtc atctatgaca ggttgtaaat
     6241 actatggaac atcatatggc gttgtcattt tcttgtcaac tatttttcct attcatgttt
     6301 ttataaaaaa acatatcgtt ttcttgtatg attaaatcac cacaaaataa acaactgaga
     6361 gaacgatatg ctttatgaat ggttctatca aaaaaattct gagatcaata gacaaaaatt
     6421 tgatgattac agaggtctct tacgaaacct tacagaagaa aaaaacattg atcgttgctg
     6481 cagttctctt ccctgctcca tgtgtctgta gaaattgtgg ttctgctgtg gtagatggaa
     6541 ataggaaatt aatggtggtg aaaaacaaga agaaggaaac cattgtccac tttgaataat
     6601 ataatcatat gccaatggtt atacgcctaa aaaagcagcg ctatacctgt aaaaactgtc
     6661 gaagccattg gaacgctcaa agttatttta tccgtccaag gcactcgatt tcaaatcatg
     6721 tcagacataa aatcacttct ttactgactg aaaaagtgtc gttatttttt atttcgaaaa
     6781 gttgttaggt atctttgaca accgttattc gcacattgaa agagttgaga agctatttac
     6841 caaagtcttc caaaagaatt ctgtcttgag tgttgagggt tgtgaatttc gttctcacgt
     6901 ttccgttgag gataaactga gttttatttc cgcggatggc gagacagaaa aattaatcga
     6961 gaaaatttac ctacacaaaa aattcctcga ttaacaaact atttccttag ctgcactaat
     7021 cctaaagaaa tagaattctt agttacagac atgaacgcca cctactttca gctcaccaaa
     7081 catgttctac caaatgcgaa attggtgatt gatcgattac ttggtttttc cacgcctttc
     7141 aaggaggcgt aacctttatt cacgagttgg ttgaagcttt ccgagacaaa agacccgact
     7201 tgtttttctc cttattgaca aagagccatt aaaactagag aaaaaattac aactgatcgc
     7261 tgattatttg tagaaagaaa atcaaagtat tacccaataa acttgagtca ttaggcaaaa
     7321 aacactgcga aactcaagaa tgattttcgc agtgtttttg tatatttttt attaactagt
     7381 attactacaa aggtttattt ttcacttctc aacgcagcac aaaattgtct ttacttattt
     7441 actggttggg ttcaaccttc tctactccga tactaatatt tgtttgtata ccccttctaa
     7501 aatcggtagt ttccattcaa ttgatgggta ctagttgatt ttaaaaacta tgcttattca
     7561 attttttatt ttagctcata tatttcgttt ctttctaaat tttatactga tgaaaacaag
     7621 tagcacaccc aatattgaca atataaactg gctaatgatg gtttctccag tttttggaag
     7681 atgcttattt ccactgcttg ttggattgtt tcctcttata tctgagctat cagcaacttt
     7741 tctattctct tttgttaaaa agatcgccac ttcttgttct ttttcgatct tgaaagttac
     7801 tggtgtcgca tc
//
LOCUS       DAYYCW010000117         7787 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000117 DAYYCW010000000
VERSION     DAYYCW010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.119"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..107
                     /locus_tag="ACHAYA_002532"
     CDS             <1..107
                     /locus_tag="ACHAYA_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFM8606283.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="ACHAYA_002533"
     CDS             complement(176..904)
                     /locus_tag="ACHAYA_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8606284.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="ACHAYA_002534"
     CDS             complement(917..2197)
                     /locus_tag="ACHAYA_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8606285.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="ACHAYA_002535"
     CDS             complement(2338..2940)
                     /locus_tag="ACHAYA_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8606286.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="ACHAYA_002536"
     CDS             3185..3391
                     /locus_tag="ACHAYA_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606287.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="ACHAYA_002537"
     CDS             3703..4422
                     /locus_tag="ACHAYA_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8606288.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="ACHAYA_002538"
     CDS             complement(4503..5186)
                     /locus_tag="ACHAYA_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM8606289.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="ACHAYA_002539"
     CDS             5405..7591
                     /locus_tag="ACHAYA_002539"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM8606290.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2793 a         1310 c         1468 g         2216 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
     7681 cttattttcg aagaagctat ttctggaaca cagtttattc ggaaaaggga ggtgtgacaa
     7741 gaattttgtc acacctccct tatttatttt gaaaatgaga atactat
//
LOCUS       DAYYCW010000118         7600 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000118 DAYYCW010000000
VERSION     DAYYCW010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.120"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..428)
                     /locus_tag="ACHAYA_002540"
     CDS             complement(138..428)
                     /locus_tag="ACHAYA_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606291.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACHAYA_002541"
     CDS             complement(546..806)
                     /locus_tag="ACHAYA_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606292.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACHAYA_002542"
     CDS             complement(943..1356)
                     /locus_tag="ACHAYA_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM8606293.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ACHAYA_002543"
     CDS             complement(1812..2297)
                     /locus_tag="ACHAYA_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606294.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="ACHAYA_002544"
     CDS             complement(2436..2651)
                     /locus_tag="ACHAYA_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM8606295.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="ACHAYA_002545"
     CDS             2875..3675
                     /locus_tag="ACHAYA_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8606296.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="ACHAYA_002546"
     CDS             3694..5562
                     /locus_tag="ACHAYA_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8606297.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="ACHAYA_002547"
     CDS             5555..6046
                     /locus_tag="ACHAYA_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM8606298.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /locus_tag="ACHAYA_002548"
     CDS             6034..6588
                     /locus_tag="ACHAYA_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM8606299.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="ACHAYA_002549"
     CDS             6606..>7600
                     /locus_tag="ACHAYA_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM8606300.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DAYYCW010000119         7549 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000119 DAYYCW010000000
VERSION     DAYYCW010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.121"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..266)
                     /locus_tag="ACHAYA_002550"
                     /pseudo
     CDS             complement(63..266)
                     /locus_tag="ACHAYA_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            232..429
                     /locus_tag="ACHAYA_002551"
     CDS             232..429
                     /locus_tag="ACHAYA_002551"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606301.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(472..1047)
                     /locus_tag="ACHAYA_002552"
     CDS             complement(472..1047)
                     /locus_tag="ACHAYA_002552"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8606302.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1202..1728)
                     /locus_tag="ACHAYA_002553"
                     /pseudo
     CDS             complement(1202..1728)
                     /locus_tag="ACHAYA_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2185..3696
                     /gene="gadC"
                     /locus_tag="ACHAYA_002554"
     CDS             2185..3696
                     /gene="gadC"
                     /locus_tag="ACHAYA_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFM8606303.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3709..5109
                     /locus_tag="ACHAYA_002555"
     CDS             3709..5109
                     /locus_tag="ACHAYA_002555"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFM8606304.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5274..6709)
                     /locus_tag="ACHAYA_002556"
                     /pseudo
     CDS             complement(5274..6709)
                     /locus_tag="ACHAYA_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            7102..>7549
                     /locus_tag="ACHAYA_002557"
     CDS             7102..>7549
                     /locus_tag="ACHAYA_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFM8606305.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT         2365 a         1277 c         1376 g         2531 t
ORIGIN      
        1 actccaaaca gggagcgtca ataattttgt gtaaataact cgtccttctg caaaataatg
       61 ggttactcag taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt
      121 ctatttaaga atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat
      181 tcttcatttt gaaactgctc ttttctgcgg ctgtatttct tgatttgttt attgaaagat
      241 tcgattgtag aaccagaagg aatggagaac acttttgaac tttacaacaa aactactgtt
      301 gataaattaa ttccaatttt agttccaact gatttaaatg gtcatgatgt tttacttcgt
      361 tttaacggat ttttaggtga ggattataga tacctaaaaa tttctgatcc tcaaccttta
      421 acagaataat aaaaaacgtg taatcaatta tttgattaca cgttttttat ttcaatcttc
      481 gttgtagtag tggttgcgac ataccgaaag gtaatcttca ttacctccta ctacaatctg
      541 ttcacctgaa taaactggaa taccatcaac aatacgagca ttcattacag cctttttatt
      601 gcaaaaatgg caaatagttt taatttcctc aattttatca gcaaatgtca ataatgcttc
      661 ggatcccttg aagagattat tttgaaaatc attcttcaat ccatagcaaa ttactggtac
      721 ttcctcattc attacgataa gtgatagatc aataatattc tctctactta aaaattgaga
      781 ctcatcgacc aaaatacaat caacttttcc gtactgatca ataatctcca ctaattctcc
      841 gtacacatcc atttcagaag taattgcaat agctggtttc gaaacaccta tacgactctc
      901 aacttttcca aatcctgatc gagtatctat tgcgcttgtc attatgataa ctctcttccc
      961 ttgttcttca taattatgag ccactttcag tagctcaata ctctttccac tattcattgt
     1021 tccatatctg taaaataatt gtgccattaa atttattccc ttcttttaca attcaattat
     1081 tccttaattc catctgcttt tatgcagctg ctcgtttatt ccttgatatg tctataacat
     1141 ctactatccc taaaaccaat ttataagcca ttagagatgg tattcctgaa catctctcat
     1201 attgtttttt gagtggtata tcatctgaaa aactgaagga atcatgaaaa tagcgacata
     1261 cttatcattt agagttctaa ctttaaatgc atttacttct tcagtcagca ctttagtcat
     1321 gttggacatg gtttggggcg tgtagtgatg cccatacatt ttctcaatca agtctgcgat
     1381 ttccgacatg gtaatacctt ttcggaagag gtgaatgacg gtctcctcta atgtgtcatt
     1441 cgtccgtcta taagcaggaa cagtctgttg cttgaactcg ccattgcggt cgcgcggaat
     1501 ctggagatga agttccccat actcggtctt gaccgtacgg tcataggagc cgttacgaga
     1561 attacccgtg ttaaaaccaa tgcgatcgta cttttcgtaa tcgaggaaag ccgttaactc
     1621 cgttttgaga agcgtattga cagctttttc caaatggaca cggaataatc cattcaaatc
     1681 gcctttgttc gctagagtct ttaggatttc tgtagtaaaa tcgttcatat ggaagtcctc
     1741 ttttctgtga atgtgttgtg gtaactctat tctacagaag ggacttcctt ttttctattt
     1801 acacaaaata ttttacactc tcaacaaaag tatcttcgat taatacttgc tcaagttatg
     1861 tgcagctacg aaaaagtagg taccgaggta ctttcttttt cgcagctttt tttggactta
     1921 tgagaagtac attcgctgga agaatacgat ataaaacgag ctttttcact aaagatatta
     1981 atagagtgaa agaaacaagc ttttgttgtc agttttttca cttttctaaa aatgaaaata
     2041 aaaaattctg gaagtggaga agaattttgc ttgctatact ttgtcttgta gaaaagaggt
     2101 agacaaaata atgcaagctc aaataagcga ttttccataa ccttggtagg aatgacaaat
     2161 taaataggaa agggaagtaa taatatgaat aaaaaaaagt tatctctatt cggttttttt
     2221 gccctgacag cttccatggt tttgactgtt tatgagtatc cgacctttgc aacatcacaa
     2281 ttacatttgg ttttctttct tcttttggga ggtattttat ggtttctacc agttgcttta
     2341 tgtgctgcag aaatggcaac agttgaagga tggaaagatg gtgggatttt cagttgggtt
     2401 agtcaaactt taggagaacg ttttggtttt gcagctattt ttttccaatg gtttcaaatt
     2461 acagtaggtt ttattacaat gatctacttc atactaggag cgctgtcgta tgtaattaat
     2521 tttccagagc ttaatacaga tccatttatc aagtttattg gtgtattagt cattttctgg
     2581 gtactgactt tttcacagtt gggcgggacc aatcgtaccg ctaaaattgc taaagcagga
     2641 tttgtcattg gaattgttat tccttcgatc attctctttg gtttagcagc agcctacttt
     2701 atcggcggta atccgattca aatggctatt aatgcgaaag cttttattcc tgacttttct
     2761 cagctttcaa ctttagtagt atttgtatct tttatattgg cttatatggg cgtagaagct
     2821 tcagcatcac atattaatga gctggaaaat ccccaaagaa actacccatt ggcaatgttt
     2881 ttactagtga ttttggccat cgccttagat gcaatcggcg gtttctcagt agcagcggtt
     2941 attccacaac atgacttgtc tctcagtgct ggtgtaatcc aaacatttga aacgcttatt
     3001 ttacatttta acagtcattt acattggttg gtaaaagtga ttgctctcat gattgcctgt
     3061 ggcgttatgg gtgaagttag ttcttgggta gttggtccat cacgagggat gtttgcagct
     3121 gctcagcgag gtctgttacc agttttcttc cggaaaacta ataaacaaga cgttccagtt
     3181 ccgttgatta tgatacaagg aattatcgtt actatttggg cagcagtctt aacttttggt
     3241 ggaggaggca acaatttatc attcttagta gcaatctcct taactgttgt catttatctc
     3301 gtaggatatt tactattttt cctaggttac tttgtattag tttttaagaa aaaagactta
     3361 aaacgaactt acaatgtacc aggaaaaact gttggcaaaa cgattatcgg tggaattggt
     3421 tttattttat cagtgttcgc attgcttatc tcttttgtac caccagcttc aattgcagct
     3481 agtgagcacc gaacatacca aatcatacta cttattagct ttgtagtgac tgttctttta
     3541 ccttttatca tttatgaagt acatgataaa agtgctcaca caactattga ggaacctact
     3601 cacgtgaaag ctgaagacgt taatcccgct gtttatccag ctgctagagg agagcaccac
     3661 gttacaaaaa atgatgaaca tattttaaaa cattaggagg aattcattat gttatacgga
     3721 aaagataatc aagaagaaaa aaactatttg gaaccaattt ttggctctgc aagtgaggat
     3781 gttgacttgc ctaaatataa gttaaacaaa gaatccattg aaccacgaat tgcttatcaa
     3841 ttagtacaag acgagatgtt ggatgaagga aatgcgcgat taaacttagc tactttttgt
     3901 caaacgtata tggaacctga agcagtgaaa ttgatgaccc aaacgttaga aaaaaatgca
     3961 attgataaat cagaataccc acgaacaacg gaaattgaaa accgctgtgt aaatatgatt
     4021 gctgatttat ggcatgctcc aaataatgaa aaattcatgg gaacttcaac gatcggctct
     4081 tcagaagcct gcatgctggg tggtatggcc atgaaatttg cttggcgtaa acgtgctgaa
     4141 aaattaggtc ttgatattca agcaaaaaaa cctaacctgg tgatctcttc tggttaccaa
     4201 gtttgttggg aaaaattctg tgtatattgg gatgtggaac tgagagaagt cccaatggat
     4261 gaaaaacata tgtcaattaa tctagatact gtcatggatt atgtggatga gtacacaatt
     4321 ggtattgtag gtattatggg tattacttac actggtcgtt atgatgatat caagggtctg
     4381 aatgatttag ttgaagctca caataaacaa actgactata aagtatacat tcatgttgac
     4441 gctgcatcgg gtggctttta tgcaccattt actgaacctg atctagtttg ggattttcaa
     4501 ttgaaaaatg ttatctcaat taattcttca ggtcacaaat atggtttggt atatccaggt
     4561 gtgggttggg tcttatggcg tgaccaacaa tacttaccag aagaattagt atttaaagtt
     4621 agttacttag gtggagaaat gccaactatg gctatcaact tctctcatag tgcagcacaa
     4681 ctaattgggc aatactacaa ctttgtacgc tatggctttg atggttatcg tgatattcac
     4741 caaagaactc atgatgttgc tgtttattta gccaaagaga tcgaaaaaac tggtattttt
     4801 gaaatcatta atgatggatc agaattgcca gttgtgtgct ataagctgaa agaagatccc
     4861 aatcgcgaat ggacactata tgatttatct gatcgtctgt taatgaaggg atggcaagtc
     4921 ccagcctacc cattacctaa agacttggat caattaatta ttcaacgctt agttgttcga
     4981 gcagactttg gaatgaacat ggctggtgat tatgtacaag atatgaacca agcaattgaa
     5041 gagttgaata aagctcatat tgtttatcat aaaaaacagg atgtgaaaac gtacggcttt
     5101 actcactaaa attcttcaga gaaatataaa agaagttaat taactaataa aaggagttgt
     5161 gattaccaca cagctcctta aatctatttg aataaatata tgatattcat ttgttacact
     5221 ctcaatctta gctgttgttc tattcatcat tactcttatt gtaagtagtt cagtcaaatt
     5281 tgcttgttca attgatcgat aacagtatga attgtttgaa aagtaatcgg atatataatt
     5341 gttttctcac tacctaagcc ttcttggtat aaagaactat cgcaaagtat aatccgctga
     5401 gtacaaaaga aagtgtcgtc tactatttct ctaagttgtt tacaaatgat attactaata
     5461 gaaaagtgtt ctcccaaaat gtcttctaac tgttgtcgat agtataaata cctaaagtct
     5521 gacggagcta ctatgaacac ttcaattgga gatttccgtt tatgctcatt taaaagactt
     5581 aaactaatgg ttagtttacg aatagaattc tcattccaac gcagaaataa attgttttgt
     5641 tttcgccatt gttcaagaat ttcatgaact tctaagtaca aagcgcgttg ctcttcattt
     5701 aacaagtaag tcttatcagg ttgaaatatc tgaatatcag cccaaagtga gcggataaac
     5761 gggagaagtg ctttatcaaa aagtatctga ttttcttcag atatattcag agataagata
     5821 agtttttgac tgagctcaat caccaaaggg ttatctgcta catggttttg ataaacaacg
     5881 ttcaagtctt tttgtaacaa ttcaaagtta tcgcatagat aatttctaga gttgaataga
     5941 aagtagatat aaaggcattc atcttcgtca tgacataaag agaaagaaag ttgctctaat
     6001 cctttctgta ttagctgaaa caagggagta tgtcgggcaa ctcttttttc ttcctgacta
     6061 aaatatattg ggttagtctt agatctctgc aaaccgatag cgatacctaa taaaataaat
     6121 tgtttttcaa ctgtagggta acttcttgaa aaaaatgttt ttctacataa tcaatcaatt
     6181 gttgaccgct ttgaataatt gatttattta gctggatcaa ctctgtccga tttgtctcat
     6241 agattatcgt tagtaacaaa cttcgataat ccttttcagg aatttcaaaa tggccatcag
     6301 catttaagta ctgcatttgt ttaaaaaaat taatcaactc atttcgtaag ttgtaaactt
     6361 tgcttaacga aatgaaggta gtatcagcta tattactcca attgtgttcc cctgataaat
     6421 aatgtcctag tacattgaaa aagtcagagc gctgatagat gagttgagtg agtgccaacc
     6481 tcgaaaacga agagtcgaat gcgactgtaa agctacttct agatttctcc actgaaataa
     6541 tttgctgatg gagtatgtcg gtaatgcatt ccaagtcgtt agtgacagtg agtgggcaaa
     6601 cgttcaattg atctgctatt tcgcgaatct gaatggtccc tttttcgtgt aattgctcac
     6661 aaatgtaaac ttgacgaaaa atacttttct ccagataggt ttccaacata gtactcctcc
     6721 tagtcatttt atacataact attataactc taccaatcgg aatagtcctg taatatattg
     6781 aatcaaaaat ataaattata aaataattag atggggtatt tggtttaagt atcccttata
     6841 tagtaataaa tttataggtg tgattctcga attaataccc gaaaatgtga gagaattttt
     6901 atggattttt taggatacaa attaattatt tttgctttta tcctgattac cagtccagta
     6961 ctgtcggtat cccattttgc agcaaaatca tgttgataaa gtgacaagaa gactgatgag
     7021 atatattatc caaattgaga agataattaa tcggattctt gagtataccc tactttttaa
     7081 gaaagtaggg tatacaagcg tatgaataaa gacataactt tccctgtgat ggacgttcgg
     7141 cttgttccaa ttaccaaaat acaggcgaac gattataatc ccaatcgagt agcgtctccg
     7201 gaaatgaact tattggagct ttctatcatt gaagatggct ttactcaacc attagtttgt
     7261 tattacaagg aaaaagaaga gaagtatatc ttggttgatg tgcttcatag gtatcgtgtt
     7321 gctaaggaaa gactcaactt aaaagttgta ccggtaacag ttattgataa acccatagaa
     7381 gaacgtatgg cttcaacaat tcgacacaat cgtgcaagag ggacccatga aataagagga
     7441 atggaaattg tcatccaaag attagtaaaa aatggatggt cagacaagag aatatccaaa
     7501 gaattaggca tggacattga agaaattttt cgctttaagc aagtgtctg
//
LOCUS       DAYYCW010000120         7288 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000120 DAYYCW010000000
VERSION     DAYYCW010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.122"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..2627
                     /gene="clpB"
                     /locus_tag="ACHAYA_002558"
     CDS             18..2627
                     /gene="clpB"
                     /locus_tag="ACHAYA_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM8606306.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2778..3464)
                     /locus_tag="ACHAYA_002559"
     CDS             complement(2778..3464)
                     /locus_tag="ACHAYA_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606307.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3442..3936)
                     /locus_tag="ACHAYA_002560"
     CDS             complement(3442..3936)
                     /locus_tag="ACHAYA_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8606308.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(3956..5176)
                     /locus_tag="ACHAYA_002561"
     CDS             complement(3956..5176)
                     /locus_tag="ACHAYA_002561"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8606309.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5323..7158
                     /gene="lepA"
                     /locus_tag="ACHAYA_002562"
     CDS             5323..7158
                     /gene="lepA"
                     /locus_tag="ACHAYA_002562"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM8606310.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
BASE COUNT         2403 a         1391 c         1490 g         2004 t
ORIGIN      
        1 taggagggta tacctgtatg aatattgaga aaatgactac gacgcttcaa gaagcgattg
       61 ctgaagctca acagattgcg gtcactcgaa aacatcaaga tatcgacatt gcccatgtat
      121 ggaaaatctt tttacaatct aatcattttg gtcgaaattt ctatacggat gccggattgg
      181 atgtcgaatc ttttgaacat gaaattgatc gtctactgga tgagtatcca gttgtttctg
      241 gtggaaacgt ccaatatgga caaaacctca gccagaattt ctttcgatta ttgaatgaag
      301 cagaccaaat acgtgaaagc ttcggcgacg agtttctttc aacggaagtc gttattttag
      361 cgttgatgaa attaaaaaat tatccgttaa ctgtttattt gaataaaaac ggattatctg
      421 aaaaagaatt gcgtaaaaac atcgaagaga tgagaggagg agaaagagtg acctcacaaa
      481 accaagaaga acaatacaaa gcattagaaa aatacggcgt tgatttagta cagcaagtca
      541 aaagcggaaa aatggatcca atcatcggtc gtgatgaaga aatccgtgat gtcatccgta
      601 ttctttcacg aaaaacaaaa aataatcctg ttctgatagg agaacccggt gtagggaaaa
      661 cggcaatcgt tgaagggctt gctcaacgta tcgtacgcaa agatgtgcct gaaaatttaa
      721 aagataaaac gattttttca ttagatatgg gtgcattgat cgctggagca aaattccgtg
      781 gtgaatttga agaaagacta aaagcagtct tgaaagaagt caaaaaaagt gatggacgta
      841 tcctcttgtt catcgatgaa atccacaata tcgtaggggc aggaaaaaca gaaggaagta
      901 tggatgccgg aaatctgttg aaaccaatgc ttgctcgcgg tgaattacat ttgatcggtg
      961 ccacaacttt agatgagtac cgtcaatata tggaaaaaga caaagcgttg gaacgtcgct
     1021 ttcaaaaggt attagtcaaa gaaccaacag tggaagatac gatcagtatt ctgcgtggat
     1081 taaaagaacg ttttgagatc caccatggtg ttaacatcca tgacaacgca ttagtagcag
     1141 cagctacttt atctgaccgt tatattactg atcggttctt accggacaaa gcaatcgatt
     1201 tgatcgatga agccagtgca acgattcggg tagaaatgaa ctctatgcca acggaattag
     1261 atcaagttac tagacgtttg atgcagttgg aaatcgaaga agctgcactg aaaaaagaat
     1321 cagatgatgc aagtaaaaaa cgcttgaaaa atttgcaaga agaattagct gaacttcgtg
     1381 aagaagcaaa tgcgatgaag atgcagtggg aaacagaaaa agaagaagtc aattctgttt
     1441 cagcaaaacg tgctgaaatc gataaagcaa aacacgaatt agaagatgca gaaaataact
     1501 atgatttaga acgcgctgct gtattgcgtc atggtacgat acctcaatta gaaaaagaat
     1561 tgaaggaatt agaagctaaa gcaaaagaca gcgagattaa gatggttcaa gagtccgtta
     1621 ccgaaaatga gattgctcaa gtcgttggca gattaacggg tatccccgta acgaaacttg
     1681 tagaaggtga acgagaaaaa ctaattaaat tgaatgaaac acttcataaa cgagtgattg
     1741 gtcaagatga agcagttgat gcggtcagtg acgcggtaat tcgttcacgg gctggattgc
     1801 aagacccaaa tcgtcctcta ggttctttcc ttttcctagg accaactggt gtcggaaaaa
     1861 ccgaattagc gaaagcgtta gctgagaact tgtttgactc agaagatcat atggtacgga
     1921 tcgatatgag tgaatatatg gaaaaacatg ctgtgtctcg tctagtcgga gctcctccag
     1981 gctatgtagg ttacgaagaa ggtggtcaat taacggaagc tgtcagacga aatccttata
     2041 caatcgtctt gttggatgaa atcgaaaaag cgcatcccga tgtcttcaat atcttgttac
     2101 aagtcctaga tgacggacgc ttgactgatt caaaagggcg agtagtagat ttcaaaaata
     2161 cggttctgat catgaccagc aatatcggtt cacagttact gctggaaggc gtgactgctg
     2221 atggaacgat tccagaagca gtggctgaac aagtcaacac gctattacgt ggaaacttca
     2281 aaccagaatt cttgaaccga atcgacgaca cgattttatt tacaccattg agcctagaca
     2341 acgtgaaagg aatcgttgac aaaatggtgg cgcaattggc acaacgtctg gaacatcaag
     2401 aaatcctgct gacaatcagt gatgaagcca aaacgtggat tgctgaaaat gcttatgaac
     2461 cagcatatgg tgcaagacca ttgaaacgat ttatcactaa ggaagtagaa acaccactag
     2521 ctaaagaaat cgttgcagga catgttatgc ctaaatcaaa agtaacgatt actttacttg
     2581 atggacaact tcatttcaaa acagaagagc tagaagaaat cgtctaatct tccattgaga
     2641 ctataaaaga aaaaaggggt ctgacacaac tagtgtcaga cctccttttt tcaaataaac
     2701 agtgttccag aagtagtttc ttcgaaaaat aagccgaaat ttcttgaagc tgcccgcttc
     2761 tgtcacaacc tcttttatta ggtatttttc actttttcta atttataact ttcaaagtaa
     2821 cgctcatctt ttttactttg agaccatgca aacaatccca ttaaggcagc acctatcgta
     2881 tttgtcagca agtcgcccat cgtatccatc aatgctgctc gtccgacaaa cagtgtgcca
     2941 tcagaagcag caaaacgttg aagattcatg cctaagaact ggtcgcataa gaattcccag
     3001 aactcccaaa acacgccgca aagtccagca aacgcaaagc cgaataagag gaacaaccaa
     3061 ggactggttt ttgatatctc agcatctttc atcaagtaac caatcaatcc atagccaaga
     3121 gctgtcagga ccattggact caccgcatgt aagattttgt cccaaaaaga gatgatactg
     3181 atcaaatgca tccctgtgcc taaaaagaca gagatgaaca agaaaaacca gtaaaacaaa
     3241 acgattgctg ctggtatttt aagtttgaaa actttcaaaa tgacttgtgg catgaaaatc
     3301 aatgcaatcc ctaataaaga ttccacgata aatgtgattc cttgagaagt cgaatatttt
     3361 tgtgtgataa attcataaaa attatagcag acaacgaata caccaaaagc aagtaaagct
     3421 atttgcacct ttttgatttc tttaatcact gaaagtccct ccatctaaaa atctttttac
     3481 ttctgaaagc aactcaattt ctgaagaaac cacttttccg ttcaatttga tcaaacctat
     3541 tgtatataag ttcaggtagt gaaattgatt ttcagctgtt tcttgtagtg cagcgatttt
     3601 tttcgggtgg gttccgcctt gctgtcgagt atcagtatat aagccgatca ctgggatgcc
     3661 tttcgcataa gccacaccga tttccgatgc taccccggca tcgatcgtca gcccatctag
     3721 taaagcaatc attaaatcac tagccaacac tttttctgtg tcagcaagag caatcattcg
     3781 actgtctgca taagcagttt tgtcattgat tgcttcattt tcttgaggaa gatagatcga
     3841 aagctcagaa gagagctctc tgatttcttt aactaactgt tggttatata acaaatcact
     3901 tttagcaaac attggtgcgg caaaataaat tttcatctaa acatcatcct ttttatcaaa
     3961 aataatcgct aaagtaagct tccatgtttg gaatgatacg ctctagtgac ttcagtgttt
     4021 cttcatctgt ttctaaccgt tcgattcttg ccaaataaga agcacggcga taattcatca
     4081 tcaagcaagt caatgtttgg atatccagtc gaacggattt tcctataggt tcatttaaga
     4141 cacggacttg tccctgttca tcccatgtta aagcgaagac accattattc caaggagcga
     4201 caggatcttc aatgatgaaa tgaaaagcat cagctgttcc aacaaacgga aaccgttgaa
     4261 ggaattcttt tacatcaacg atccgagcca taaaatatgg ttcgatctgc tctttgatct
     4321 gactatcttc aatcaaaaat gctaaaggct catttttata aatatctcca tgtacccagt
     4381 agatcatgga aaaatgtgcg gaaataaagt tccataagcc atttcgagct tcttgatcaa
     4441 gatagaacat ttcttttacg tgaaatattt cttcagctac ccagtagaac agaacacctc
     4501 ttggttcatg gttgctatcg tagtagacag cggctgtgcg ctcatcttca ttttcgaaac
     4561 gccaatactc ttcccaatga aaatcactgc ggaataaagc accatggttt tgttgggcaa
     4621 atttgtcata aacggaaaat acgtctggat gatcaactgg taatcgctca accataccac
     4681 ttactgggat ttgtttaggt agctgtgtat ctctgatttt aaaggaaagt ttatcagaca
     4741 tgatttccca tcctttacgc cgatagtagg ggatactgta aggatacaaa taagagatcc
     4801 attgcttatt cttgcgcata ttatccaatg caacgataat tagatcttgc atcaatccat
     4861 ggttagcata ttctggataa gttccaactc cagtcacacc acccatttta taacgtgcac
     4921 cgtgaatatt gacttcacaa ggatagatag cgatttgaga gatcagttta ttttcgtgga
     4981 accatccgaa cactttcgat acttccaaaa tcggtttttt ggaacggata aaagctcgtt
     5041 tattctcaaa cccgctttcc tcgatgtctg cttcagtcac ttggaacaca taggaaagca
     5101 attcattgaa ttgatcgata tgttcttgag tgaccggttt taattcaaga ttttctctaa
     5161 aatctttttg atccattttt tcatctccat ttattagctt gataacagta tagcaaaatt
     5221 ctacaaaaaa aacttctata gctgactaat taaaaactct ttaaattact gcttttcgtt
     5281 ggtataatgg aaagtgctga ttatatgaaa agaggagacg ctatggatat aaagaaaatg
     5341 aaagaacgcc aagagcagat tcgtaatttt tcgatcatcg ctcatatcga ccatgggaaa
     5401 tctacattgg ccgatcgtat tttagaaatg acgcacactg tgacgtctcg cgaaatgcaa
     5461 gatcagctgt tagactcgat ggatttggaa cgtgaacgtg gaatcacgat taaattaaat
     5521 gctgtagaac tcaattacac agcgaaaaat ggagaaacat atatctttca cttgattgac
     5581 accccagggc acgtcgactt cacctatgaa gtctctcgaa gccttgctgc ctgtgagggt
     5641 gccgttttag tggtcgatgc agcacaagga atcgaagcac aaacattggc caatgtttat
     5701 ttagcgttag acaatgattt ggagatcctg ccagtcatca ataaaatcga cctacccgca
     5761 gcagatcccg aacgtgtccg tcaagaaatc gaagacgtaa tcggaatcga tgctagtgaa
     5821 gctgtattag ccagtgccaa atcagggatt gggattgaag atattctcga gcagatcgtg
     5881 gaatatgttc ctgcaccaag tggagactta gatgcaccgc taaaagcttt gatctttgac
     5941 tctatttatg atagttaccg cggtgttgtt ctaaatgtcc ggatcacgga cggtatggtc
     6001 aaacccggag acaaaatcaa actgatgagt aatggcaaaa catttgatgt tacagaggtc
     6061 ggggtcttct cgccaaaagc agttgctaga gactttttga tggtcggtga tgtcggttat
     6121 atcacagcaa gtatcaaaac tgtccaagat acacgagtag gggatacagt aacgttagca
     6181 gacaatcctg ccgctgaagc tttaccaggt taccgaaaaa tgaatccgat ggtttattgt
     6241 ggtctttatc caattgatac ttctcgttac aacgatttgc gggaagcttt agaaaaactg
     6301 caattgaatg atgctgcttt acaattcgaa ccagaaacct ctcaagcatt aggttttggg
     6361 ttccgctgtg gattcctcgg acttcttcat atggatgtcg tccaagaacg cttggaacgg
     6421 gaattcaatc tagaattgat cacgactgca ccatccgtta tttaccatgt taataaaacg
     6481 gatggtacga ctgctacagt agataacccg gcagattttc ctgaacctgt aacgattcag
     6541 gatgtagaag aaccattcgt caaagcacag atcatggtcc caaatgattt tgtcggtgca
     6601 gtaatggaac tttctcaacg aaaacgggga gaattcatta cgatggatta tttagatgat
     6661 taccgagtaa atgtcgtcta taatattccg ctttctgaaa ttgtctttga ctttttcgac
     6721 aaattgaaat caagcacgaa aggctatgcc tcattggatt atgaaatgtc tggttatcaa
     6781 aaaagcaagt tagtgaagat ggacatcttg ctgaatggag aaaaagtcga tgcattaagc
     6841 tttatcgttc accgagactt tgcctatgaa cgtggaaaag caatcgttga aaaactgaaa
     6901 aaactaatcc cacgccaaca atttgaggtg ccaatccaag cagctattgg acaaaaaatc
     6961 gttgcccgtt cagacatcaa agccttgcgt aaaaacgtct tggctaaatg ttatggtgga
     7021 gatgtttctc gtaaacgtaa actcttagag aaacaaaaag aagggaagaa gcggatgaaa
     7081 caaatcggct ccgtcgaagt tccgcaagaa gccttcatgg ccgttctgaa aatggatgag
     7141 caagatagtg tgaaataact tataatcaat cgaaaaacct attaaataaa cctttttgag
     7201 tagatgcaac actgaggatt atctgtataa aagattttaa actgtcattt ggttatcacg
     7261 gcgatactaa aattaacaaa attggaat
//
LOCUS       DAYYCW010000121         7141 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000121 DAYYCW010000000
VERSION     DAYYCW010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.123"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..615
                     /locus_tag="ACHAYA_002563"
     CDS             <1..615
                     /locus_tag="ACHAYA_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8606311.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            612..1385
                     /locus_tag="ACHAYA_002564"
     CDS             612..1385
                     /locus_tag="ACHAYA_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8606312.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1382..1780
                     /locus_tag="ACHAYA_002565"
     CDS             1382..1780
                     /locus_tag="ACHAYA_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFM8606313.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1965..2636
                     /locus_tag="ACHAYA_002566"
     CDS             1965..2636
                     /locus_tag="ACHAYA_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606314.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2695..3135
                     /locus_tag="ACHAYA_002567"
     CDS             2695..3135
                     /locus_tag="ACHAYA_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606315.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3147..3815
                     /locus_tag="ACHAYA_002568"
     CDS             3147..3815
                     /locus_tag="ACHAYA_002568"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8606316.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3879..4502
                     /locus_tag="ACHAYA_002569"
     CDS             3879..4502
                     /locus_tag="ACHAYA_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM8606317.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4558..4944
                     /locus_tag="ACHAYA_002570"
     CDS             4558..4944
                     /locus_tag="ACHAYA_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM8606318.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5157..6548
                     /locus_tag="ACHAYA_002571"
     CDS             5157..6548
                     /locus_tag="ACHAYA_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM8606319.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         2277 a         1160 c         1572 g         2132 t
ORIGIN      
        1 tttgtttttg caccgttaga aggagaagac ctcgcttcat ttctggttgg tgcggcaatc
       61 atgatgatcg gaatgacgct ctttttgttt ggagcagatt attctatgat ggaagtcgga
      121 gatctagtag ggaaatatat gatcaaaaag aaaagtttga ccgttttggt cagtttggga
      181 tttgctatcg ggatcgtgat cacgattgct gaaccttccg tgcaagtttt gggacaacaa
      241 gtatatgata tatctgacgg agagattggc agagtgttgc tgattgggat cgtcagcgtc
      301 ggcactggag tatttctagc ctttgcgctg cttcgggtcg tattcaagct atcctattat
      361 caattgatgg ctataggcta cataggcgtt ttgatcgcct catttttcac cagttccgaa
      421 tttatgccga ttgcttttga ttcgggggga gtgactactg gtccagtaac tgttccattt
      481 atcttggccc ttgccggagg gatgactagt atgatcaaac aaaagaaaaa tgaaaatgac
      541 agttttggaa tggtcggtat tgcttcgtta gggccgattc ttgccgtgat gattttgggg
      601 gtgatcttcc aatgagtttc tggcagcaag tgatagatgg aatcgaaatc gttaccatgg
      661 aagtcatttt ggcagtggca cctatcgtaa tcctatttgt tttaatgaat atcattagtt
      721 ttcgtatgcg caaaaagcgt ttctttgaca ttatgaaagg tttcttgata acgactattg
      781 ggttgatcct tttcctgcac ggagtcaata tggcttatgt accggtagga cagcatttag
      841 gcagtgctat tgcgggattg ccttacaatt atatactggt tccccttggc tttttaatgg
      901 ggttcttagt agggtttgct gagccggcga ttcatgtaat gatcaaacag gttgaagaat
      961 tgagcgaggg gagaatccga tcgaaaatca tgctgatggt aatttcaatt gggatcggcg
     1021 cagcagtagg attgtctatg tggcgtttgc tcgtcggatt ctctctttat tatttcttga
     1081 tcccaggata cgggctagca tttattttag gacgaaaagt ggacaagatc tttttagcaa
     1141 tggcttttga taacggtgga gtagcaacgg gcccgatgtg ttcgacattt attttatcaa
     1201 tgagcgtggc aatcgctgca caaattgaag gaagagatcc gatcattgac gggtttggtg
     1261 tggttgcaat gattgcattg acaccaatat tatccacttt agtactagga tatatttata
     1321 aacgaaaaga tgaaaaagac agaatcgaaa aagaaagatt gcaaaaattg ggggaaagca
     1381 aatgaaagat cttgtagcat tgaatcttca catgattgtg acgatcgttg acggaggaat
     1441 gggcgaagat gttatcgact tttcaaaaga agcaggtgca gcaggcggaa cgatcttaca
     1501 tggaagaggt tctggtgtac atgattcagg gaaattttta ggtttggaca tttcgccaga
     1561 aaaagatatt gtgttgacat tagtacccga tagtttgacg aataaagtaa tggatgcgat
     1621 tggtcaaggc atcaacatcg atattccagg taatggtatt tgttttagta ttgatgtaga
     1681 caaagtcatt ggtatcacta agattcatca atatcgagaa attatcggaa tgcaagaact
     1741 tttagatgaa aataacgata gagaaaagtc tgaatcatag acaagcgttt gaagaggaaa
     1801 taaacacaaa caagaaggcg gatgagcgtt atttccgctt ttttgtttgt atttttctcg
     1861 tgaatactta tgaaatagta aatttttatg aaattctact ctaagcatca gactttcttt
     1921 tcaaaatatg ctattttaaa aatgatgaaa aaagggggag aaaaatgaat tttttagttg
     1981 ttttaaaaga tactctgatt gagcgattta catttctata tgttcaattg caggaaaata
     2041 aaacattgct gcatttttca ccagagttcc atctagaggg gctaacatta ggagaaatct
     2101 atcaagcaga agggctgtcg gctgttttgc acttttttaa ggtagaattg gaattaccag
     2161 taaaagatta tatcgagctt tctcttgaat cttgggatcg agcagtggtt gaactgtttc
     2221 cagaaggttt aatgatcgat acaaacgatg ggaagatcca tctgactgca gcagaactgc
     2281 aaaaacaaat gcgttatcaa atagatgacg aaaattcttt ttctattttt agacgtcagc
     2341 aaaagattct aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga
     2401 cacagctgtt gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt cctcaattga
     2461 ttacgatgat ccatcgtttt atacgtatgc aacagctacc atctaagaag ctgtttcttc
     2521 cattgacgga cacatttcga gtagtcaaca gagaagataa aaagaaagtg attgtgattg
     2581 actttaccag aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc aattaaggct
     2641 ttttcttttt ggattttttt gtcataataa atattagatg atggaggaga taatatgatg
     2701 caaacatggt tagaaaggct aatacgattt cagcagaact atcacaaact tatgcaggga
     2761 agatatgcga aatttgatca gttgaatcgc ggtctattga tcgctgcgct tgtctttagt
     2821 ttgctaagtc gttggttgcc ctatagaatc ggacaattgt tgttcatcgt ctttttcgga
     2881 tggatgatat ttcgtttcct atcaaaaaaa atctacccca gactaaatga gaatcaacgt
     2941 tatttgaaat atcttgatag agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa
     3001 gtcaaaatgg atcgaaaaag ttatactttt tttgagtgcc cgaattgtca tcaaaagcag
     3061 cgtgcaccta aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg tataaaattc
     3121 gaaacgaatg tttaagggag aaaagaatga aattagtatt attacgtcat ggcgaaagcg
     3181 aagcaaattt tgaaaattac tggacaggat ggctagatgt cgccttaaca gaaaaaggaa
     3241 aagaacaagc agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg
     3301 tttatacctc tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg
     3361 gtcagttatg gcttcctata ataaaaacat ggcgtctaaa tgaacggcat tatggggatc
     3421 ttgtgggtaa aaacaaagat aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat
     3481 ggcgcagagg atatacggaa gttccgccgt taaccgcaga aaatagattt gaccgcagat
     3541 atgatcactt ggatccgcat ttgatcccat acggagaaag tctggaaatg acagtgaatc
     3601 ggatcattcc cttatggcaa gatcatctag caccaaaact aaaagatcgg caagatttac
     3661 tagttgtggg tcatggtaat agcatccgag cactgacaaa atatttagaa gatgttcctg
     3721 aagatcaaat ggatacgatc gatatcccaa atgcacaacc aatccagtat acattagcag
     3781 atgatttgac aattctggac aaaaaaatac tttaaagaac acctttatct aaaagtgaga
     3841 gatagaaaaa agaagcaaag gaatggaaca aaaggaacat gaaaaattat atatgggact
     3901 ttgatggtac attgtttgat acgtatcccg caatggtaga tggtgcatgg caagcactaa
     3961 aagattttgg tatcagtatg gacaaaaaag aaatctactt caaaatgaaa aagtattcta
     4021 ctagttattt gatcaatgaa tcaaacttga atgccagaga gtttaatgaa ttatttcacc
     4081 gatatgaaaa agaaagtacg gaagtttccc gtccatttcc agaaacgaaa caagtactgg
     4141 aaatgctaaa agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa
     4201 catggggcct gttaaaagaa catcgattgg cacatctgat tgaggaagta gtagggatcg
     4261 atcaggactt tcctcgtaaa cctgatccag catctttaaa ttatttgatc gatacttttc
     4321 atttagaaag aacagacacg atgatgatcg gcgatcgtcg tttagatatc gaagcaggga
     4381 aaaatgcagg agtcgctact tgtctttacg acatcgatca ttttctgggt gagatcccgg
     4441 cagattatgt ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc attaaaaagt
     4501 agtatcccct ttcaaaggag atatgttata attttcataa aggagtgata aaattatatg
     4561 gaaatcagaa gacaaaaaga agttgtagaa gcatttcatt atgatatgag attgccgaac
     4621 caagaagtag aaacagattt gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac
     4681 tatccgaaag aacatagcat cgtaggagca cgcttagagt tccgtttagt gtttgataca
     4741 tatgttttat ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa aattgaaggt
     4801 caaaatgatg tcacacaaga agaagtggac gagctagtag caccgttatt cgatatcgta
     4861 cgccgaatgt cttatgaggt gacagagatc gccttagatc agcctggggt aaaattgaat
     4921 tttcaatcag aaaaacaaga atagacagta aaaacatacc tgcgatagat taaagctatc
     4981 acaggtatgt ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg
     5041 tctaaaatat aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa gaatcattct
     5101 cgggaacctt tacttccttc gatggacttg tcattaataa tatgaggagg agaaaaatga
     5161 caacactcat cacagtttta gtggttctag tttttgcggc attattatat gtgttctatc
     5221 gtatgcaaaa gcgccataca aaattttcaa cacgcgtctt tacagcatta ggagttggta
     5281 tcgtctttgg cggtctgctt caattgatct ttggtgtaga cgacaaagtc gttactcaat
     5341 caatggaatg gatcgggatt gtaggtcaag gatatgtatc attcttgcaa atgctagtta
     5401 tgcctttagt gtttatttcg atcattggtg cattcacaaa aatgaaagtt tctgaaaagc
     5461 tagggaaaat tagtgcgact gtattgactt ctttacttgg aacaacagct attgcagcat
     5521 tgatcggaat tggttcaaca atgctgttcg gactccaagg agctaaattc acacaaggag
     5581 ctgcagaaac agcacgaatc tctgaactgg caacacgtca ggaaacagtt gaaaacttat
     5641 cgattccaca gcagatttta tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta
     5701 gtcgtgcaac aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc
     5761 taggcgtgaa aagaaaagcg ccaaaagaag cagcattctt ttcagattta gtcgacagtc
     5821 tgtataaaat cattatgcgg atcgtgacgc ttgttttgcg tttgacacct tatggagtat
     5881 ttgctttgat gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc aatttaggta
     5941 aatttgtcgt tgcttcttac gtagcattgc ttgctgtttt gattgtccac tcactgattt
     6001 tgattggtgc aaaagtcaat ccaatcaact atttcaaaaa agcctttccg gtattgagct
     6061 ttgcgtttac ttcacgttca agtgctggag ctttgccatt gaatatcgag actcagacaa
     6121 aagctttagg agtagatgat gcaacagcta atttctcagc aagttttggt ttgtctattg
     6181 gacaaaacgg ctgtgctggc gtttatccag ctatgttggc aacgatcgtc gcaccagcag
     6241 ctggagtgaa tgtctttagt ttggaattta ttttgatgct tgtggcaatt gtaacaatca
     6301 gttcttttgg tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg atcgttttag
     6361 gggcaatgaa cttgccagta gcaatcgttg ggttagttat ctctgttgaa ccattaatcg
     6421 atatggcacg tacagcagta aacgtcagcg gtagcatggt agctggtatc gtgacaagtg
     6481 cacgcatcaa agatttagat cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga
     6541 atgcatagtc aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata agaagaagtt
     6601 tcctaaaaat agtttctcaa atttttgtga aatttcggct tatttccgag aagctacttc
     6661 tgaaacaccg tgaataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
     6721 caaacaaaaa tagttcctca tattttacgt ttggttgagc ttattaccgc agatgcaagc
     6781 ttttgaacac cgtttaagaa agaggtcgtg agcctgccgt ttcactctga gtcacaaaga
     6841 gcaatttcaa aaaatagctt ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt
     6901 gtcaggagaa caccgtttac cagaggttgt gacttgtttt tagtcacgac ctcttttttt
     6961 atttattcca tataaataca cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa
     7021 caaccaactt atttattatt aaagaaaagc gtatttaagt aggacgattc tgttgagagc
     7081 tttcgtagat tttcccattt tcctatggta aactgacaaa gttgagaatg ttagaacaga
     7141 t
//
LOCUS       DAYYCW010000122         7067 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000122 DAYYCW010000000
VERSION     DAYYCW010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.124"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            239..1096
                     /locus_tag="ACHAYA_002572"
     CDS             239..1096
                     /locus_tag="ACHAYA_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8606320.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1089..1991
                     /locus_tag="ACHAYA_002573"
     CDS             1089..1991
                     /locus_tag="ACHAYA_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8606321.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            2024..2374
                     /locus_tag="ACHAYA_002574"
     CDS             2024..2374
                     /locus_tag="ACHAYA_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8606322.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2423..3346
                     /locus_tag="ACHAYA_002575"
     CDS             2423..3346
                     /locus_tag="ACHAYA_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8606323.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3424..4317
                     /locus_tag="ACHAYA_002576"
     CDS             3424..4317
                     /locus_tag="ACHAYA_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8606324.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4355..4918
                     /gene="amaP"
                     /locus_tag="ACHAYA_002577"
     CDS             4355..4918
                     /gene="amaP"
                     /locus_tag="ACHAYA_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8606325.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4932..5120
                     /locus_tag="ACHAYA_002578"
     CDS             4932..5120
                     /locus_tag="ACHAYA_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8606326.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5133..6035
                     /locus_tag="ACHAYA_002579"
     CDS             5133..6035
                     /locus_tag="ACHAYA_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8606327.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            6067..6309
                     /locus_tag="ACHAYA_002580"
     CDS             6067..6309
                     /locus_tag="ACHAYA_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8606328.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6565..6936)
                     /locus_tag="ACHAYA_002581"
     CDS             complement(6565..6936)
                     /locus_tag="ACHAYA_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606329.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2577 a         1194 c         1402 g         1894 t
ORIGIN      
        1 tgaatttttt caaaaaacat tcttaagaaa aaggtggcta tatatagtag tttttggtca
       61 atacctttga ataaaaagcc atgaaaaacg aatagttagc gcttaccgat gcgttttgtg
      121 ataaaaaaat tttaaaatat tcaatgaaaa caaagcatat attttgcttt aaaaccaaat
      181 gaggaatatt ctagatgtag aacaggttag aaaaagatta gaaaaaggag tgattttgat
      241 ggagaagaga tacggcgtag ttcctgtggt aactgcttca gacgaaaatt atgctcctta
      301 cttgagcgtg atgatcgcaa ctgcattaga aaattgcaat aaaacaagac gcataaaatt
      361 ttatgtgatt gacgatggtc tatcagaata tagtaaagaa ggattggaag aaacggtaaa
      421 taaatattca agtaatgcaa gtatccaatt cctgacagtg gaaaaagata tctacgaaga
      481 cttcctagtc agtgaccata tcacaactac cgcttactta agaatttctt taccaaactt
      541 attagctaaa gaggattata agaaagtcct ttacttagat tcagatgtgt tagtactaga
      601 cgatatcgta aaactatatg atgagccgtt aaatggaaaa acaatcggcg ctattatcga
      661 tccaggccaa gtgaaagctt tggaaagatt aggaatcgat tccgatgact tgtacttcaa
      721 ttcaggagtg atggtgattg acattgatca atggaataaa aaagaaatta cagaaaaaac
      781 tatccattac ctcagtgaaa atggcgatcg aatcatctat cacgaccaag atgctttgaa
      841 tgctgtactt tatgaagatt gggaacaact tcatccaaaa tggaacatgc agacttcgct
      901 gatttttgaa cgtcatccag caccaaatga aaaatatgaa cgtcagtata aagaaggaaa
      961 tgaaaaacct tcgatcgttc actttacagg acatgataaa ccttggaata cgttgaaaga
     1021 tcatccgtat accaatttat acttaaaaaa attagcacac agtactctaa cgaaagtagg
     1081 cgagatcaat gaataaaaaa gaaatagcag tagtcgcaag ttgtaacaca aaattcgtac
     1141 cacatttagc tgcattattt gtctcagtat tagataattg caacccgtct aaatttgttc
     1201 ggttctatgt gattgacgat gatatcgatt ttgaaagtaa acagctttta cgcttctcag
     1261 tgaaaaacgc acgaatgaac tcagatgtgg aattcttgaa aatcaataaa gaattcttta
     1321 caaatgtggt aatcagcgat cgtattccag aaacagctta ttatcgtatt gcgattccgg
     1381 aattgttccg cggcacagaa gttgaacgta tcttatatat ggattgcgac atgatcgctt
     1441 tacaagatat ctctaaatta tggcgtcttg acttcggtga ttcaatcgta gcagctgttg
     1501 aagacgctgg gttccatcaa cgactggaaa aaatgaaaat ttcagctaaa tcaatgcgtt
     1561 atttcaactc aggtttgatg ctgatcaatg tgaaaaaatg gttagatgaa aatattacgc
     1621 aaaaagtatt agatttcatc gaacataatc cagaaaaatt acgattccat gaccaagatg
     1681 cattaaatgc tatcttacat gaccgttggt taccacttca tccgcgttgg aatgcacaag
     1741 gctatatcat ggcaaaagca aaaaaacatc caacagcagc aggtgaacgt gaatacgaag
     1801 aaactcgcaa caatccttac attatccatt tttcaggaca tgtgaaaccg tggagcaagg
     1861 attttgaagg gccaacgaag aaatattacg aaaaatatgc agggatgacg gctttccgtt
     1921 gtgtagctaa attccctaaa tatccaaaat atgcaaaagt tcaaccaaaa acagatgtag
     1981 ttgttaaata atagaagacc acaactgaag agggggagta ggaatggaaa ttacaatgtc
     2041 taaaaatgca gtagagacgt taattgaaaa ggttggggaa aatacaaaaa tcgcactcgc
     2101 gcttatcaat gattccgatc catttctaag ggacaaaggt gcttttgcaa aaggcagctt
     2161 ttttcaaatc attccttttg tttcggaatt tggagaatat gcgactaaaa tcgagcatcc
     2221 attattggac atctatacat ctaaattaga gcaaaattat ttcggtaaac gattgaacat
     2281 ggacttcaac aaacagcttg acagcttttc attagaaaat gaaatcgcag tactcgatta
     2341 taatatcaaa ttaaagaact gcttcttcag ctaatagttt cgagaaagtc caaaaaggag
     2401 gtatacattg agatactact ttgtggatga agaaaagaat gtaatagaaa cagatgaaaa
     2461 aaaatataat tggctcgtac ttgatagtgc aaagcccgaa gagattgagc aagcaatcga
     2521 agaattcgag cttcctgatg atattttcgt aggaaccgaa tatccggaag aagtttctcg
     2581 gatggagcgc ctttatgaga cgaaactaga gcatccattt tctttggtag tgattaatct
     2641 agatcagtca gataatcgta tcgaaaaaaa attaacgcca atatcttttg tactttccga
     2701 taatctcttg attacttgta tcgatagaaa aaccgaattt gtggatcggc tgctgacaaa
     2761 gcatggaaat catttaaact cgtttgaaaa gatcatcacg tatacattat tagatattta
     2821 cacccattac gtgaaagaac taagagagat gaaaaagcga attgacgctt tggataaagc
     2881 agctcgtaaa acaacggaaa atgaagaact gtataaacag gcagatcttg aaagagacat
     2941 cgtatatatc gatcatacct taagagatca acgggaaaca atggatcaat tatggaattc
     3001 tgaaactttc atcaaacgtt tagacgatga acagctactc tatgatgtcc atttgagaca
     3061 gcgacaaaca gaaaaaatga tcgagatcta tcgagattta ttagagagta ttggtgatct
     3121 attcaacggg atgatggaca ataatctaaa tcatatcatg aaatatctag attcagctgc
     3181 attaatcatt tccgttccgg cgttgttcgc agggatttgg ggaatgaata caggaggatt
     3241 accgggtaaa agttctgctc taggctttat actcgtagtc tttggctctt tgatagcagc
     3301 agtagtttta ggttaccatt tataccggaa agattataca aattaaagaa tctatttaaa
     3361 acaggtatca aacaaagaaa tttgttttgg caatacaatt taaaaactag gaggaataca
     3421 gcaatgacag aaccaaaatt aatcgacccg cgaaacttgt accatacgga gaaatttcct
     3481 aaacaagatc aggatacacc agctttacag cagaatatga ttcctgttcc tgattgtgga
     3541 gaagaatcct atgaaggcaa taatcaacta gaaaaccgcc gtgtactaat cactggtgga
     3601 gattcaggga tcggtcgtgc agcagcgatt gcatttgcta gagaaggggc agatatcgct
     3661 cttcaattct tcccaggaga agaaaaagat gcagaggaag tagcaaaata cgtgaaagat
     3721 gcaggacgta agattgtctt actacctttt gatcttcgtg acgaacaagc acctaaagaa
     3781 atcatagata aagccgtgag tgaacttggc gggctagata cgatggtact aaacgcagga
     3841 cagcaaattt cttgtcagtc tattgctgag ttaccacttg agcaagtcaa agacacattt
     3901 atgataaaca tcatcgctat gtttgcttta gtgaaagaag ctgttccgca tatcccagca
     3961 ggaggagcaa tcgtaacaac aacttctgtt caagcattca atccaagcga acatttatta
     4021 gattacgcag caacaaaagc ttcaatcgct aactttacga tcggtttagc aaaacagctt
     4081 gcacctaaag gaatccgtgt aaatggtgta gcacctggtc caatttggac accattacaa
     4141 ctagacgaag gacaaccttc tgattcgatc cctgaatttg gtcagcaaag tttactagaa
     4201 cgtgcaggtc agccaggaga attagcacca gtttatgttt tcttagcatc gaataaagca
     4261 agttatgtta cagcccaaat ttacggagta actggtggag aagcgatcaa cttataagaa
     4321 gttgatcgtt tcacccctat gaggaggtgc gaaaatgaat aaaggtgtaa aaacgatcgg
     4381 agtgatcgga acattacttt tgctatctgt gcttttgttt gcgatactat taagtgcaac
     4441 ctatccattg ccttttcagc tagagaagtt tcgattcttt tccatcacta attcatacgt
     4501 gcaacaatat gtattttggc ttgccgttgc atttgctatt ttgacaatta tcacgttact
     4561 tgttttcctg ttttatccaa aaacaatcgg aaactttcta ctgaaacatg gtgatgggga
     4621 gttgacgttg gataaaaaag caattgaagg aatggttcgt tctcacttgc atgaagatga
     4681 attcatccat tcaccaaaag taaatgtcaa agcaacgaaa aacaagatcg ccgtcaatgt
     4741 aaaaggggag ttgaaacgaa cttcagcact cattggtaaa acaggaacat tgatgaaaga
     4801 aatcgaaaat gaagtacaac acatattggg aacaaaagag ccagtgaaag tatcggtgaa
     4861 atacacaggc tttgaagaac cagctaaacg aagcaatgac cgcaaacgag tagaataggg
     4921 ggggaccaaa aatgaaagaa ctattttcga catacaagct ccctatcatc tttggggtac
     4981 ttggattgat attagccgtt ttatggatca ccgtgggctt tttcaagacc ttattgatct
     5041 tgatcatggc agcgatcggc gtcgctatcg gtttttatct gaaacaaacg aaaatcttag
     5101 atcactattt taaatcttag gaggaaacat ccatgaataa caataacaaa aataataaca
     5161 ataaaatgaa caaccaaaaa ccagtgtctg cacatccaga cccaacgaca ccaaaccaat
     5221 caaatggtgt gaacgatgaa aagaacaaaa cagcaccagt atctgcacat ccagatccag
     5281 tagtccctaa cgttcaacaa ggagtagaag agaagaaaca tgatgcagaa aagcatgcac
     5341 ctcatcatcc aggaactcca gaatctaaag ttccagaaca acatgatgca atcgatcctt
     5401 ctaaacctgt ggaagagcaa aaacatcctt ctgaccatgc gaaagcacaa gaaaaactta
     5461 gttctgacca cacaaacgga caaggaaaac ttggttctga tcatacaaat aaccaaggaa
     5521 agcatgattc tgaacaggaa gattcaatgc gcggtgagct gaattatgaa gataaagttg
     5581 tgcaaaaaat catcggaatc gcaattgaac aagtagacgg tttattatct gcaaacggtg
     5641 gtttcttctc taatgtggca ggaaaacttg tgaatacaga taatgtaacg gctgggatcg
     5701 aaacagaagt cggtaaaaaa caagtagcag ttgatatgga tgtcattgtg gaatatggca
     5761 aagatatcga aaaaatcttt gaagaaatgc aagaagtaat cggcaaagaa gtaaaaaata
     5821 tgacacattt agaagtgatc gaagtcaacg ctaacgtagt ggacatcaaa acaaaagaag
     5881 aatttgaaaa agatcaagaa acggtgcaag acaaagtaac tgacgcagcg aaaaaaaccg
     5941 gtgaatttgc ttcgaatcaa acagataaag taaaatcagc agctggaagc ggcgcacaaa
     6001 aagtaaaaga aaatacagaa ccacgggttc agtaataagg aagtaaaaca ttaggaggaa
     6061 cgaaacatgg gatttatttg gtcacttatc gtcggaggaa ttatcggagc aattgcagga
     6121 gcaatcacga ataaaggttc ttcgatgggg attattgcaa atgtcgtagc aggtttagtt
     6181 ggatcagcac ttggtcaagc attattaggt tcttggggac caagtattgc aggaatggca
     6241 atcgtgccat cattgattgg tgccatcatt ttagtactaa tcgtatcatt ctttgctcga
     6301 aaactgtaaa aagactttta aaaagaggtc gtgaaaaagc tgttcttcat caagtaataa
     6361 gaacagccaa acaaaaatag cttctcatat ttttcatttg gttgggcata ttatcgaaga
     6421 tgcaagcttt tgaacactgt ttatacggaa taagaggctg tgacaagact tttgtcacag
     6481 cctcttattt atgcgggcaa attcccaatt agatttttaa aagtttttcc ttaaatgtgt
     6541 ctttattgat acagagaagc ttctttaata aaacaatgcc tccctaaatt ttcttgagat
     6601 aggaaaggtg aacatacaaa ataatacgac cactttgaaa acccagatga tccacaaatg
     6661 gatcgtatga tactgatgaa gtaaaaagca atggatcaga aactcaaaga gattgaaaaa
     6721 gacaaggaag aaataatatc tcttttttac tcttgtctgt tttagccaat aatagctcgt
     6781 aaagtaatct agacaaacaa atagaagtaa acaaagtttc gtaagagcag caatcgtatc
     6841 atgttgctct gctagacgat tgacgagcag gaaaaatgtt ggtaatggaa tatagatcac
     6901 gagtaatgaa agaaaaaagt aaacaataaa tggcatacta ttcctcctaa ttgtgtgagt
     6961 tcgccctcat tttaaaataa cgatatttgt ttgtgaagct ttttcgtgtc gtaacaaatg
     7021 ttatctaatg tatttttaaa taaataatca ttattatcaa aataatg
//
LOCUS       DAYYCW010000123         6644 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000123 DAYYCW010000000
VERSION     DAYYCW010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.125"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACHAYA_002582"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACHAYA_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHAYA_002583"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHAYA_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM8606330.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACHAYA_002584"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACHAYA_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACHAYA_002585"
     CDS             complement(2143..2373)
                     /locus_tag="ACHAYA_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM8606331.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACHAYA_002586"
     CDS             complement(2603..3421)
                     /locus_tag="ACHAYA_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8606332.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACHAYA_002587"
     CDS             3582..4271
                     /locus_tag="ACHAYA_002587"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8606333.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACHAYA_002588"
     CDS             4285..5787
                     /locus_tag="ACHAYA_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8606334.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACHAYA_002589"
     CDS             5800..6276
                     /locus_tag="ACHAYA_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8606335.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACHAYA_002590"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACHAYA_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAYYCW010000124         6595 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000124 DAYYCW010000000
VERSION     DAYYCW010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.126"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..503
                     /locus_tag="ACHAYA_002591"
     CDS             123..503
                     /locus_tag="ACHAYA_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8606336.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            599..1108
                     /locus_tag="ACHAYA_002592"
     CDS             599..1108
                     /locus_tag="ACHAYA_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606337.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            1185..1364
                     /locus_tag="ACHAYA_002593"
     CDS             1185..1364
                     /locus_tag="ACHAYA_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606338.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            1419..1982
                     /locus_tag="ACHAYA_002594"
     CDS             1419..1982
                     /locus_tag="ACHAYA_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8606339.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(2029..2544)
                     /locus_tag="ACHAYA_002595"
     CDS             complement(2029..2544)
                     /locus_tag="ACHAYA_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM8606340.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(2570..3439)
                     /locus_tag="ACHAYA_002596"
     CDS             complement(2570..3439)
                     /locus_tag="ACHAYA_002596"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8606341.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            3565..4245
                     /locus_tag="ACHAYA_002597"
     CDS             3565..4245
                     /locus_tag="ACHAYA_002597"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8606342.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4398..5378
                     /gene="pta"
                     /locus_tag="ACHAYA_002598"
     CDS             4398..5378
                     /gene="pta"
                     /locus_tag="ACHAYA_002598"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM8606343.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            5501..5974
                     /gene="tsaE"
                     /locus_tag="ACHAYA_002599"
     CDS             5501..5974
                     /gene="tsaE"
                     /locus_tag="ACHAYA_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM8606344.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            5967..6491
                     /locus_tag="ACHAYA_002600"
     CDS             5967..6491
                     /locus_tag="ACHAYA_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM8606345.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
BASE COUNT         2185 a         1166 c         1391 g         1853 t
ORIGIN      
        1 tgtttttttt gccttttttc ttataaaaag gaaaaacagc tatttttttc aatctgacac
       61 atttacaatg gaactggaga ttaatataga tccgagctta aacgaagaat aggagtgctc
      121 atatgaacct tacaacgatc catcatatag ccatcattgt ttcagattat cagaaatcac
      181 gtgattttta tgttaataaa ttaggatttg aagtaatcag agaaaattac cgcaaagaac
      241 gagatgacta taaattagat ctgaaactag gaagtgctga attggagatt tttggaattg
      301 ctaatgcgcc acaacgacca aactatccag aagcttgtgg cttgcgtcat cttgctttca
      361 aagtagacga tattgaagaa acaattgcag aattgcagtc cttagggatc gaatcagaac
      421 cgattagaga agataccttt acaggaaaaa aaatgacgtt tttctttgat ccagacggat
      481 tgccattaga actgcacgaa taaagataac aaaattgtca cttttttttc gtttcattcg
      541 atttttcatt cgttcatttc ttgatattct ttaaaaaaaa gaaacaggag aatgaagaat
      601 gaaaaaaatg atgtttggtg ttgctgccct catcgtaata gggattaccg gtatgacggg
      661 actgctcaat gccgcttttc acatggatga tattagtgac caggaatatc ggatctcatt
      721 gaaaaaagca gcagcaatcg taaaaaacga aacaaaagaa cataacatat ctgctattca
      781 ttttgcagtt gaaaaagatt tggagaatcc tacgaatgca acatatgaat accagttttt
      841 ttgtgaagga caagtcatag cagtcaatcc atccactggg aaaacaaccg tacgaaatga
      901 cacggaaaag gccgcagaac atctttttga tatagaaaaa acggaagagt tcaaacaacc
      961 gcaagcagcg atgaaggaag cagtcagcta ctacgggaaa aaagcaaaag tgaaagagtg
     1021 gacacttcta gtcaaagatg aacgtcctta ttataaagtc cgattaatag atggagaaca
     1081 ggaccgtcag gtatggatat ctgcatagaa gaaggatatt gcttctcttt tttacaatat
     1141 aagagtaagc taagagagta aagatgaaag gaagttgaga taacatggaa aaaatgaaat
     1201 gccctaattg cggaaaaaaa tttgcatacg aagaagtcaa taatgtagtc gaacataatg
     1261 ataaagaaat gccagtcgtc tgcccttatt gccgaactga agcggcaaga atcgttactc
     1321 atggttattt cattactgaa aagattgaag attttttaaa ataacgattt tgcgatttct
     1381 ttttctgcat gttagaatgg agaaaaggag aggaaactat gattcgtttt gcgagaaaag
     1441 aagatgctcc agaaatcgcc ccattgatct tagtgattct aaaagatatg gaactgccat
     1501 ttttgaagca gacaggggaa caaaagactt tagagatttt agaagaagca atcactgatc
     1561 caaattatcg ttacagttat tcacgaggta tcgtagaaga aatagacggt aaagttgcag
     1621 gagttgctta cggttataca gatgaagaag aatcaatcat cgatcagcca ctggtaccta
     1681 tcttagagaa atatcagcta gatgcgtcta cacgtttgtt cattgataaa gaaacgtttc
     1741 ccaacgaatg gtatttggac tctatcagtg tatcagaaga ttttagagga caaggcatcg
     1801 gttcgcgatt attagaagca ttacctaaat tggcgaagaa agctaatcgc tccgtcatcg
     1861 gtctaagtgt ggatgaaaaa aatccaaagg ctaaaaaatt atacgaacgc catgacttca
     1921 aagttgtcgg tcagcggatg atcagtggtc atctttatga tcatatgcaa aagaatatct
     1981 aaaaagatgt acctgaaatg cgtatttccg catttcaggt acatcttttt aacttaagtt
     2041 aagtcggtaa gaaacaggct tatcgcgact tttgtcttta cggtagagcc agtaatctaa
     2101 aaccgcagca atacaaatac aaactggagc atcttcatca tttgagacat ccaatacata
     2161 gtaatctcct gaaaaaagtg ttgctttatc cattttcatg atttgctgat tgaaatgatg
     2221 gatttgatat tgatgattat agatatcacc ttggactgtc cagtgaagtc tacgaatata
     2281 ataaaagtca ccgggccaat tgaatatttt ctgcatggtt cccacctttt cgaactcttt
     2341 atacagctca aaacgcgtac cgaatgtcag gcttatctgc ttgatgtgag caacgagatc
     2401 gccattcatg gcataaagag acaatgcatc gccctttgtt ccccagcgac caaccatcaa
     2461 aaacagggaa tgtccttttt cgtttttgac aattgtccga gttgcacgtc caagctgctt
     2521 ttcctgaatg aaaaattctg acatctatat ccctcttttc tagataaaac tacaagtctt
     2581 ctgcaatcgc gcgcaatatg gcttttccta cgccagattc aaggtttgtg tccgttgtat
     2641 atttggcata atcttttact tcgatttctg catttcccat tgcgaaactg actcctgcta
     2701 cttgcagcat gctgacatca ttgaagttat cgccaatcgt catgacttct tctaaagaaa
     2761 tcccacgctc gtgagcgaca tgggcaacag caattccttt ttgagcgttt ttatggttca
     2821 cttcgatatt gttttgacca gaggaagtaa cggctagatc gcctaaaaga gaaatctgct
     2881 ttccaactgg acctaataca gctggccctt ctgtatgaaa acaaataatt tttaaaactt
     2941 ccagattctt ttcttccaaa atagcacgaa tactgtccac gtatgtgatg tgcaataact
     3001 ccagcttagc tgaggccatg gcaatcgcca ttttatacgt aaggtgaggt aaatgggtag
     3061 ccaccatgga agcaaaactt tcaattcgtt tcgcttgatt ttcagaataa agaccatcgt
     3121 tggttgctac ttcatagtaa ataccgttag catctagaat atctaaaaca gagattgctt
     3181 gattatttgg aatcgacacg gtaaacaatg aatttccttc tttatcaaat acctgtgccc
     3241 cgttcaaagt gatcatcgca caatcgattc ctacctcatc taaagctgcg cgagcttctt
     3301 gatagtttcg accagtagcg accatgaact caacaccggc atcatttgca aaacgtatcg
     3361 cctcggcatt ttcaggagaa acactcatat gggcatctaa taatgtgccg tccatatctg
     3421 atgcaattaa tttgatcata atgattatcg ttcctttctg cttcttgttc ctagtttacc
     3481 atattaatca agtgctgagg gtgaagattg tcccttgtga ttgaagaaaa tgtgagttat
     3541 tctttttatt aggaggtgga gtttttgaaa gaaatcattc ataacagttg gcaagaagtt
     3601 ttgtcctcag aattttcaaa agattattat ctgcatctaa gagaattttt aaaaaaagaa
     3661 tatgcgtccc aaaagatcca tccagatatg tatcatatct atgaagcatt ggaattgact
     3721 ccttatgaag aagttaaagt ggtgatttta ggacaagatc cttatcatgg agaaaatcaa
     3781 gctcatggct tatctttttc tgtgcaacct ggcgtaaaga tcccgccatc gttaagaaat
     3841 atctataaag agctttatga tgatcttgga atcgcacctg ttcaacatgg aaatctagtg
     3901 tcatgggcta aacagggagt ccttttactc aacaccgttc ttaccgttcg ggaagggcaa
     3961 gcgtattctc atcgcgggaa aggttgggaa agattaacag acacgatcat tgaaaaatta
     4021 aatgaacggg aaaaaccaat cgtgtttatc ttgtggggaa aaccggcaca agaaaaaatc
     4081 aaaatgatcg ataagagtcg ccatatcatc ataacatctc cacatcccag tccgttatct
     4141 gcaagtcgtg gcttttttgg ttccaaaccc ttctcgaaaa cgaatgatgc gctccttgcg
     4201 ttaggagaag aaccgattga ttggcaactg cctgaaacag tgtaataaaa cagcgctttc
     4261 ctgttatagt gcagttcgct ttgtatgtga aaaacttaat ttttccagca tttttgactg
     4321 atacagataa aataaactgt tatttttttc tggaatagtg tatgataata catataaaaa
     4381 cgtctggagg tcatcttgtg gaattgtttg atagtttaag atttaaaatt gttcgccgtg
     4441 ggatcaaaat cgttttcccg gaagctactg atgctcgtat tttaggcgca gctgcccggt
     4501 tgaaagcaga agaacttgtg gaacccattt tagtagggaa cgaagaagaa ataagaaatg
     4561 cagcgcatgc cagaggaatc aaaacatcta gctttacgat catcagccct gataattacg
     4621 ataagtggga tgaaatggtg gatacttttg ttgaacgtag aaatggaaag gcatccaaag
     4681 aacaagcaga aaagatttta aaagatgtga attactttgg tacgatgctt acgtatatgg
     4741 ggattgcaga cggaatggtc tctggagcga tccattcgac tggcgacact gttcgcccag
     4801 cacttcagat catcaaaaca aagcctggca tcagtcggac aagcggagcg atgatcatgg
     4861 tacgtggaag agatcaagaa aaatatgtct tttcggattg tgcgatcaac gtgaatccaa
     4921 ctgcgcaaga attggctgaa atcgccgtag attctgcaaa aacagcagaa ttgttcgata
     4981 tcgaaccaaa agtagcaatg atgagcttct cgacaaaagg ctcagcgaaa tcgccagaag
     5041 tagacaaagt agttgaagca actaaaatcg ctaaaaaact agcgccacaa tacatgatcg
     5101 acggcgaatt gcaatttgat gcttcttatg ttccttcagt tgcacaactg aaagcgccag
     5161 attcagaagt agctggtcaa gcaacagtct ttgtatttcc agaattgcag tcaggaaata
     5221 tcggctataa aattgctcag cgtttcggta actttgaagc aatcggacca attctacaag
     5281 gcttgaacaa accggtttct gatttgtctc gtggagcaaa tgaagaagat gtatataaat
     5341 tatcgattat cacagcagca caaacattga ttgattaaca aatacacgcg aactagccaa
     5401 aaacagcagc tgctgttctt gactagttca ttttttcttt aaaaaaactt cattacgcga
     5461 tatactggca aaaagaaaga agagcgggag aagatgaaaa atgatcgaac tatcaggatt
     5521 aacaatgaca gaacaattcg cagaggcaat tggtcattcc gcaatgccag gtgacaatct
     5581 gatattgact ggtgatctag gtgcgggtaa aacaacgttg acgaaaggaa ttgcccaagg
     5641 attagggata acgcaaatga tcaaaagtcc gacatacacg attattagag aatatagtca
     5701 aggtcggatt ccgttgtatc atatggatat ttatcgggta gcagctagcg gagcagattt
     5761 agggttagat gaatattttg aaggtgatgg gctttccgtt gttgaatggg gaaatctatt
     5821 agaagaagca ctgccggagg attatttgga actgatttta gaaaaaagtg atacagattt
     5881 agaaaagcgg tatgtgaaac taatctcata cggggagcaa agtgaaacct tcaaacagcg
     5941 aattctagaa aaatgggctg aaaatcatga gtgaagaagt tgggatcatc ttgcgggaag
     6001 cagtgccagg agatgcaaaa gacattcttt tgatgatggg acaagttaat aaggagacag
     6061 aatttttagt tttggatgaa gcagaactcc tgttaccacc agaaacctta gaagaagagt
     6121 tggattatat atacgaaagc aataataatt tattgctgct tgcaatctat gaagggacga
     6181 ttattggaac agcatctgtc aaggcagaca gtcaatttcg tttgtctcac gtgggagaag
     6241 tcggtatcag tattctgcag gaatattggg gaatgggact aggcactctt atgctagaag
     6301 aaatcatcag ttgggcgaag gaaatgggtg tactttttcg attggagctt gacgttcaag
     6361 ttcgtaatga aagagccgta cacctttacc gaaaaatggg cttccaaatc gaagcggtca
     6421 tgcctagagg ggcaagaaca gatctaggtg aatttttaga tgtatataaa atgagttatt
     6481 tgattgaata gaagaaatag gaaaagagtg agaaactgtc atccttttga gaaggcagaa
     6541 aaacacacga agggactgtg acaaaagtcg tgtcgcagtc catttttttt gaata
//
LOCUS       DAYYCW010000125         6386 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000125 DAYYCW010000000
VERSION     DAYYCW010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.127"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..671)
                     /locus_tag="ACHAYA_002601"
     CDS             complement(<1..671)
                     /locus_tag="ACHAYA_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8606346.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKIT"
     gene            873..1172
                     /locus_tag="ACHAYA_002602"
     CDS             873..1172
                     /locus_tag="ACHAYA_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8606347.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            1437..2312
                     /locus_tag="ACHAYA_002603"
     CDS             1437..2312
                     /locus_tag="ACHAYA_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606348.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            2309..2527
                     /locus_tag="ACHAYA_002604"
     CDS             2309..2527
                     /locus_tag="ACHAYA_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606349.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            2514..5141
                     /locus_tag="ACHAYA_002605"
     CDS             2514..5141
                     /locus_tag="ACHAYA_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM8606350.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            5156..5374
                     /locus_tag="ACHAYA_002606"
     CDS             5156..5374
                     /locus_tag="ACHAYA_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606351.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
BASE COUNT         2449 a          810 c         1205 g         1922 t
ORIGIN      
        1 gtaattttat aaaagaaatc ctcgacagat gaatactttg aaatatcttt ataggctaag
       61 tccatttcgt ctaatagctg ttttaaagaa tcaatcattc tttctttttc accgtatttg
      121 aaatcaccat ttcttatctt gtcttgaagt ttactgttgg ctcttttgaa aaatagctct
      181 ttagagatag agtgtctttg gttagcgtac tcttcaatca gaggattgat attattagca
      241 agtatctcat ttagataaat ttcaaaatct tttgagtaat attttgtaaa tatatttttt
      301 attataggat tcaataactc atagtcttta tctagcgaaa acgtatcact atatctttct
      361 tgaatgtctt taaagacttt gtgaggtatt tctgggaagt ccttaatata tagctcatac
      421 cctatatcga taaggtatga agttatatat tcttctaaag ttccccattt aatccagtca
      481 attgttgaat tagtgatagt tgataataat ttcagctttt gtggatttgg taaattggtc
      541 ccacgttccc agttggatat ggtcgcacgt ggtgaattat ctattttcct tccaagttct
      601 tccatagtaa aaccacatga aaggcgtaat tgtttgattc gttttcctgc ttctgaagga
      661 tttggcttca tcttttttct cctttttatt tttaaagata gtatatcata tttataaaat
      721 aaaagtattt aaaagtattt actttttatc tggagtatga tactttattc ttataaatac
      781 aaataaatac atttttttac gcaatttgta tttatttgta cttataaagg tagaggagtt
      841 atagaataat taattaaaaa ggagaggttt ttgtggaagc aataaaatta atgatttcag
      901 aagaacagga ggaaatgttt ctaaagcagt tttatcaaat tgctacaaaa gctattgaaa
      961 aggctcaaga aaatgcgggg atgacaaggg aatatctcaa tcaaaaagaa atggcggaat
     1021 acttaaatgt gagtgaaaac tttttaaaaa atgctgtgag aaatgaggga ttgcccgtag
     1081 ttgaattagg ggcaagaaaa ttttattcga aaaaggcagt taatgagttt atgtttgctc
     1141 gaatgaaaag aggggaaaat attggaagat gatgaagtag aaattataaa aaaatgcttt
     1201 aaaaaaagaa aaaaagtgtt cccaaaagtt tgcagactgg gggaacactc aataataaag
     1261 tgctgagaaa actttattta tagctattat atcaaaaaag aaggtgaaaa ggtgttttct
     1321 tctggaaatg ttgtagttga caaacttaga gaaataaatt ttgacggaaa tgtaatccac
     1381 acacatggta cgatcaatta agaaaaaaaa cgaaaaaagg tgttgagaag ccctatttga
     1441 atgcaatagt tatcttagca gaaattacct attggtatag acctaaaaaa atttttaacg
     1501 atgatggtca gctacttggt tatcaaaaaa agtttcttga agatattttg caaaaatcat
     1561 ataagcaatt aagcaaaaaa acaggattat cagaacgagc tataaaagat gcaattgtgt
     1621 acttagagaa aaaggggatt atcaaaaggg tttttagaaa tatttattat gagaaaagag
     1681 cagttacatt atcaaatgtt atgtacattg ttttaaatgt acataaattg atagaaatta
     1741 cttttcctac tttggattgc gaaaaaaagg agtatatgcc gaaaattcgt cagaggggta
     1801 tcgaaaaaaa gagatatgtc tctttaaaaa aaggtagagg tatgtacaaa tttgtatata
     1861 cgtatacaaa gactacttca cagaacatat caaagactac tctcgttatg gaagaagagg
     1921 atattaatat aaagaggatt tacagtaaga ttagtgttct ttatgacgca aataacgaag
     1981 atggtcgaaa attatttcaa gtgtatcact atttgagaaa cgctaatgta gatacagaag
     2041 atatcagaaa aatcattgaa tatttatctg aaaatactca gtatcttatt gctgagtgta
     2101 ttataaaaca acatgaagca tgcttttttc aaagaaaaac tgaagaaggg ttatacgatt
     2161 atgtaacata ttttttgaat ggacttcaga aaagatataa aagtcaattt ttagaaaatg
     2221 tagagcctat agatcagttc aaaaaagctt tcgaaatcaa agagttacct gaaattacta
     2281 tgcataattg gttgaaagga gaagaatcat gattaaaaat cgcttaatag atcaaatgtg
     2341 gtgtcttgat caaaatacag atactctcga attaatcgag agtatctgct tcaacacagt
     2401 agttctcgat ttaagagctg aaaatgacaa ttgtgtaact catatcataa ttaatcaaaa
     2461 aatggctcag cagctctctg agagcctaag aaaatgggct gaaggagaaa actatgaaag
     2521 caattaaaga aaaaatcgtt agtagaaaaa taaactggtt agcactcagt gtacttgttt
     2581 taatgattag cgttgttttt acaagtaata ttgttgaagc tagcattttc aattggtttg
     2641 atgacacaaa aagtcaaaca gagtttttga ataattctct ttatagacct tacctaaaaa
     2701 aatacaatgg agatttcctt agtcagttcg ggatttggct tggatgggct gtggtaaaag
     2761 gcatgtttac tgtgactgat tcgattcaaa atatgattcc agatgtgttg gatttgttca
     2821 atttcattga aagtacaggg ctaaataatg tttatcaatc agtcataaat acaattgtcg
     2881 ttggattaat gatcctttca ttgatgtttg ttggctacaa gatgattact ggaaaaggaa
     2941 caatagattt aagatctgtt ggaatgaata tagtgatgtc tgtagcgtta attcttttaa
     3001 tgccaacaat gatttctagc ggtattcaat ttgccaaaat attttacaat gatgctacta
     3061 cgattacaaa ttcagatgac ggagtcgctt ggtcattgat aaaacaagga gtaacagatc
     3121 tggcatatat taataaaaca gatcaatact caagcattga taaaacggag gatcgaaata
     3181 agttaacacg aaaaaatttt caacagacag atttaacaca agtgttgacg gataaagtga
     3241 ttgataaatt ggaaaaagaa aatccagctg cggataatct tcgctatgaa ttagttgaaa
     3301 attctaataa tgaatttgtt gccacaaagt tttctgataa cttcttatca acattttctg
     3361 atagcttgaa atcaggttat tatcgatatc aagcaaatct ttgggggatt tcgattgggt
     3421 tgacagcgtt ggcaatcgcc tatgtgttca gcgcgtttgt gatcattaca gcaatattag
     3481 aacttgcatt taaacgtgtg ttaggtgttc ttgtttttgc gacagatatt gaaacaggac
     3541 aacgttcaaa agttgtttta tccgatattt tacaatgtta cttaacagtt ggatttcaag
     3601 ggtttggact atctatgttt gctatgttta tcaatttttt aaatgctgga caaggtatct
     3661 ccacaaatat ttttataaaa acaattgctt atatctgtgc tgtgtttgtg ctgataaaag
     3721 gcagtggaac agtcatgaga tactttggtg ttgatattgg actcaaagaa ggctatgggc
     3781 aacttgcttc tgcttttggt atgggcgcaa tgctgtttag aaaaggatca actggattta
     3841 atcgtgctaa aggaagcgga aatggaaatg gatcagacag tggagaagga gaagatcgaa
     3901 aaccagaaaa gaatttcggc gaaactctat caaaaaaagc tggcaaaact ggaagagctt
     3961 tgggatatgc ccatgaacgt ggattatcag gtttagcttc agatggagct acaatggcta
     4021 gtgaaagagc aataaaacca tttaagagta tgcgtgatat ggctaatgat accaagaata
     4081 agttcaaaga aggtttagat gatggtacag tgtccgctat taataaaaat agcaaaccta
     4141 tgttagctaa aaataaagaa gatgaaactg gaaaatatgc tgattccatg ccaacacgtc
     4201 tttcggatag aaaagatggt gctaaggttg aaaatgccga tagaattatg tctagttctg
     4261 aacgtatgcg tgaagcaatg aaaaataatg ctgaatcgaa taataatcct gtaagtgcaa
     4321 ttcagcaaaa agttcaacaa gatattgaag aacgtaaaaa tgctatgcac ggtcaagcta
     4381 agtctgctga agaattaatt aatcagaagc gacaagaagc taaatacacg cctgaagcaa
     4441 tgaatcgtga ggaaatgtta aggaaacgtg tagaagggcg tacaggagct aatacggacg
     4501 aaaaagaagc gttgaccaaa gaaagaatcc aagaagctaa aaactcaaat accggattag
     4561 aaaaattggt caaagaaaat ctacaaaatt ctacaagtag tcaaggtggc cgatcagtag
     4621 atgtccgaga aaatattcaa ggttcattga atggtcatgt cggacggaat gttgatgtaa
     4681 gagagaattt acaaagctct tcaaatggtc aaggtactaa gacaattgat gttcgagaaa
     4741 atgtacaaaa ttctacaagt agtcaagggt ttaaatcaat ggatgtaaga gagaatgtac
     4801 aaaattctac aagtagtcaa gggtttaaat caatggatgt aagagagaat gtacaaagtt
     4861 ctacaagtag tcaaggattc aaaacagtag atgttcggga gaacgttcaa aaagatagtg
     4921 gagaaatgaa agaaaaaaca caaaaaatca atatcgttga agacagaaag tcagcgaaaa
     4981 aatttgattc aaatcatgaa acagtgatta ttgatagtga aatccgtgaa aatgataagg
     5041 gatcaaaacc aagacgaaga tttacctatg aagacaacga gttattcaga gatacactta
     5101 atgatcctaa tccgcttttc gataatctat taaaaaaata aaagggggaa ttgtcatgaa
     5161 attaattcta aagattagta cgcatgattt cttagaactt gacgaattat gtgaatcagt
     5221 aaagaacgca acaaatgaaa aaatcacggt cactaattat attgaactga tgattcaaga
     5281 agaattaaag atgaaacatg agataaaaaa caacttgaaa tattgttatc tagaaaaaag
     5341 tgaagagata atccatgaat taaatggctt ttaaaaattg tatttgacat gaatttacga
     5401 caaggaatta gtggggatta agcgattatt ctcttccact ttagagaaat aatgggttaa
     5461 aaagccagaa tacgtagagc aatattgaca acctataaag gagctattgt tgaaaattga
     5521 agtaacaaaa agataaaggg aggtttacat ctatcagatt aaaagtgata gtacacccat
     5581 gaaacctgca aaagagttga acctaatgta tatcggttta actcttttta tttgcaaatt
     5641 aaaaaaatat caccgaagtg acgaatgaaa ctatgaccaa attaagcaag aaaatatatc
     5701 ttgcaaataa agtagtataa aaagatattg ataaagccaa tcaaaacttt gaatttcaaa
     5761 cgttagataa tcaaacagat acaaaaaatg aaatcagttc tacgcgtacg attattaaaa
     5821 ataataaatt ttcactagaa gaaaatggaa ataaaatcaa ataattagga agaaaaaaca
     5881 aagcaattag aaaagcctgc aagcaaattt gagtaatcga gatccaattt ttttaaggaa
     5941 gtttaacaaa aatatgtaat tctattatca ctattaaaga gaaagttgga gcggaatata
     6001 aagaaaagag aaaagtaatt aaacgcaaaa aaatgtagaa cttaaaaaat ataaagagga
     6061 acaaccgtta aaattaggaa tgatgttgtg taaacattat aattgctatg atataaatga
     6121 agtgttaaag aaagcaaatt taaaccaatt ttgtgaacag aaaagcctta gtcactatga
     6181 ccaaaactac ggacgaaact atgaccaaaa gtaggtacta aattatgacc gaaaataggt
     6241 ccaaaatgga tttttttatg atatcaaaaa ttatgaaatg attttgaaag aaaaccagtt
     6301 ggcacagcca acttagccac cttcctgtag gaaggtgcta tcagttttcc cttatgctct
     6361 tttctttctt ttttgtttca tatgtc
//
LOCUS       DAYYCW010000126         6354 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000126 DAYYCW010000000
VERSION     DAYYCW010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.128"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..1219)
                     /locus_tag="ACHAYA_002607"
                     /pseudo
     CDS             complement(89..1219)
                     /locus_tag="ACHAYA_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     misc_binding    complement(1279..1506)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1868..3070)
                     /locus_tag="ACHAYA_002608"
     CDS             complement(1868..3070)
                     /locus_tag="ACHAYA_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8606352.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3130..3333)
                     /locus_tag="ACHAYA_002609"
     CDS             complement(3130..3333)
                     /locus_tag="ACHAYA_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8606353.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3475..4542)
                     /locus_tag="ACHAYA_002610"
     CDS             complement(3475..4542)
                     /locus_tag="ACHAYA_002610"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8606354.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4600..4707)
                     /locus_tag="ACHAYA_002611"
     CDS             complement(4600..4707)
                     /locus_tag="ACHAYA_002611"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8606355.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4870..5682
                     /locus_tag="ACHAYA_002612"
     CDS             4870..5682
                     /locus_tag="ACHAYA_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8606356.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6056..6247)
                     /locus_tag="ACHAYA_002613"
     CDS             complement(6056..6247)
                     /locus_tag="ACHAYA_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606357.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1893 a         1303 c         1004 g         2154 t
ORIGIN      
        1 tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg actttttggg
       61 acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg acttgctgca
      121 ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct tctgttgaag
      181 caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt tgtgcttctt
      241 ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa gtaactaatc
      301 ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa tctcctgctc
      361 ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac tcttctgcaa
      421 tgggttcata tccaccggaa aacagctaag aagaagcgtg attcccgcgg cagatatgtg
      481 ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc tctgtacaaa
      541 tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt ttagtttgat
      601 cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct ttttcataaa
      661 aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca gatagatcag
      721 gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt cgtaaaatca
      781 tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc actacttctt
      841 tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat ttatctacta
      901 cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat ccagtttctg
      961 gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa aatccacgcc
     1021 gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc actaattttt
     1081 ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct tcttgaactt
     1141 tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga tctactgtaa
     1201 atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa aaaaaatcct
     1261 tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt ttttcagaaa
     1321 aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga aagactgttc
     1381 gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt caacttctag
     1441 ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc ttctcatcgg
     1501 atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag ggaataagaa
     1561 caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat aaagcttttc
     1621 taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa atgatcatac
     1681 ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa aactaagtcc
     1741 tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac atcttgaaca
     1801 ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg gtagaaccgt
     1861 ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc tcttgaacat
     1921 tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg taagttccta
     1981 aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt tgaatattct
     2041 gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc ggtttgacac
     2101 ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc gtaaatgttc
     2161 cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca taccactgtc
     2221 tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat ttagttttgg
     2281 gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca atcgttatcg
     2341 tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca atccgaatgc
     2401 cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc ttatcatcct
     2461 tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact ttcttattct
     2521 cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc tctttaacag
     2581 cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat tttgataacg
     2641 tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt tcaaataacc
     2701 aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa aactgcatac
     2761 gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt ctctcgtacc
     2821 attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta gaacacagtt
     2881 gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa gcaattttgg
     2941 catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca ttttcatcta
     3001 tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa atatttttaa
     3061 tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat agtaccgcgc
     3121 ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc gggatggcac
     3181 cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa gatatgcctt
     3241 gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta tcaaaattgc
     3301 acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt ctttactcct
     3361 tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt tatactcgtc
     3421 ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc ttttttagta
     3481 gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa cctcctcctg
     3541 ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg gattcatttc
     3601 tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga cctgtgcaat
     3661 aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct caaaactttg
     3721 aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg ctgcccattt
     3781 ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa ttgctccatt
     3841 aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct ttgccaagct
     3901 ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg cctcttctaa
     3961 aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa aacgacactg
     4021 actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta tgatatccgt
     4081 accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta ttaccttatg
     4141 cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat caatccgtgt
     4201 aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg tttttcgtgt
     4261 ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca cagttccttt
     4321 aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt attggccaat
     4381 atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt caataatctc
     4441 ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat caatcaaaac
     4501 acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca atttttcttc
     4561 acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa tccccacttt
     4621 ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa tggcttcacc
     4681 gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt acttgtcaaa
     4741 gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta ttttaagtca
     4801 atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt atcctgagga
     4861 ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca gattcgccaa
     4921 gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc gcgaagtaca
     4981 gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca ccaaatcgca
     5041 gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa tcaatacatt
     5101 ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg tctcatagta
     5161 gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat gattgaattt
     5221 tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca atgtatcgat
     5281 gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca gctctatttg
     5341 caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc caactttagt
     5401 gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt tgggaaacat
     5461 ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca attgaccagc
     5521 gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca gtacacagag
     5581 gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat ggaagtcgag
     5641 aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa aattgataag
     5701 cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca cagtttcctg
     5761 ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg ctacgccttc
     5821 ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg acctggagtt
     5881 tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc ataatcagcg
     5941 cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct ggttagttaa
     6001 tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc aacctttaaa
     6061 aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca ttctcaaatt
     6121 ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat ttccattttt
     6181 gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg aaaaattttt
     6241 gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct caaattattt
     6301 ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt ccta
//
LOCUS       DAYYCW010000127         6347 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000127 DAYYCW010000000
VERSION     DAYYCW010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.129"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..1135
                     /locus_tag="ACHAYA_002614"
     CDS             290..1135
                     /locus_tag="ACHAYA_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8606358.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="ACHAYA_002615"
     CDS             1416..2636
                     /locus_tag="ACHAYA_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8606359.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="ACHAYA_002616"
     CDS             2633..5353
                     /locus_tag="ACHAYA_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8606360.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="ACHAYA_002617"
     CDS             5337..6281
                     /locus_tag="ACHAYA_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8606361.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2363 a          983 c         1224 g         1777 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttc
//
LOCUS       DAYYCW010000128         6313 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000128 DAYYCW010000000
VERSION     DAYYCW010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.130"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2504
                     /locus_tag="ACHAYA_002618"
     CDS             <1..2504
                     /locus_tag="ACHAYA_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFM8606362.1"
                     /translation="EGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2686..2925
                     /locus_tag="ACHAYA_002619"
     CDS             2686..2925
                     /locus_tag="ACHAYA_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606363.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            2941..4314
                     /locus_tag="ACHAYA_002620"
     CDS             2941..4314
                     /locus_tag="ACHAYA_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8606364.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4318..5952
                     /locus_tag="ACHAYA_002621"
     CDS             4318..5952
                     /locus_tag="ACHAYA_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8606365.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6115..>6313
                     /locus_tag="ACHAYA_002622"
     CDS             6115..>6313
                     /locus_tag="ACHAYA_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606366.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2208 a         1033 c         1282 g         1790 t
ORIGIN      
        1 ctgaaggtac agtaaacata acttataatc ctattaaaca agaggtgatt acacaaaaaa
       61 agcattctgt gagagacaat gatattgaaa aaattcggtt tgtatttatt gatattgacc
      121 ctaaaaggaa agagggaagt gcaacagata gcgaaaagaa aaaggcagaa aatgttatgg
      181 aacaagtaga acgttatttg aaagaaaaga gaatagaatc atttgtaaaa gttgatagtg
      241 ggaatggcta ccatattttt cttcccataa atgaacaacc aaataatcac gagacgattc
      301 ttactattca gaattttctt caattacttc accgaagatt tggagtagaa gatgaagttg
      361 atattgatac ttctgtttat aatccttcaa gattatgcaa attagttggt acaccagcgg
      421 tcaaaggaaa gtccacaaaa gagcgtccgc atcgtttatc tagtttcctt tctattcccg
      481 ataacataga tagaagtaat tgttttaaaa ttcttgaaca gatagttgaa gaaaattcca
      541 tgatgtcaac ctctagagta aaaagccatc catcttttag agaagtgtta ccttttattc
      601 aagcagacta taaacaatgg ttagctcact atcctgaatt aacttatacc ataaaagagg
      661 gcgatcataa aggagttacc ttacttatat ttgacgaatg tccattaaga aaacacacga
      721 ataattctaa tggttcttgt ttgtctgtta cggctgataa taagattcga tttcattgtc
      781 tgcatgcttc ggataaaaat aaagatattc aagactttgt aaaaaaatat ccgttgccaa
      841 aacctgtaaa tccttctgta gaatcattag aatccaatca agaatattat tttgaccagt
      901 ttaaactgaa tcaagaagga gtatttttgg tgaaagaagg tcaagaagaa aaagttgctt
      961 gtccgatgtt tattaagcaa atacgcaaaa atatcgaaca aaaagtcatt acacttgttc
     1021 ttgcctatta tgtcgatcat tgttgggatg aaatagaaat agaaggtgga tgtttacaaa
     1081 ctaataattt taaacaatta gcaaaacatg gcttaatcat taaagctaga agagaagctg
     1141 aagtcgttga ctttttacag attcaacggg agaatgctcc aatctttttt gaacatgaat
     1201 ctcttggctg gaaaaaagaa aatgaagcaa tgatttttca gttacaagat tcttttggac
     1261 gtgaaattcc ttctgtatta actaaaaact catgctacca attaacaact aatggtacag
     1321 aagcggaatt tgacgcattg gttcataagt atgttttagg aacgaactta gaacttgctt
     1381 ggtcgattgg tattgcttct attgtgttag gctatttaaa cgttacgaag gctaatactt
     1441 ttttgagttt actaattaac ctacagggca agagcacaac gggtaaaacc actacgttac
     1501 actttattgc ctctttattt ggaaatccaa ctacggttct tagaaatatg aatgctacca
     1561 ataaagccat tataaagcta gcatcaaaca atcagggagg tgttccacta atactagatg
     1621 agttaggagc agcaactcaa aataacttta gctcgttgat ctatcaatta gcttcaggag
     1681 aagaaaggct aagattagat tctaattgtc agcttatgcc acaagagact ttttctgttt
     1741 tgacccttac ctcttctgaa gagcgtttgg aaaattattt caatgagaaa ttaactggtt
     1801 tgaaagttcg atatatcgaa ttttctgata tcaagtttac aaaggatgcg aatcacgcag
     1861 agaaagttaa aagtctttct tctaatactt ttggttgggg tataaagtta tttcttttga
     1921 aactctttga acaagggatt acaaaaattg aagcaacttt tgtcgaagct caaaaaaaac
     1981 tatgggctgt tttacctgaa cattctttga agcaacgcat tggtaataat ctagctttga
     2041 tttatagtgg agccctccta attcaagata tactgggcta tcctttacga ttagagcata
     2101 tacagacggt gcttattaat tcgtacgaac agttaattgt agatactagt ttaggaagtg
     2161 agcaaacggt atttgaccga gtgaaaacat tgttggtgat gaatagccat aaatttttaa
     2221 caaacagtga caacaaacat acgcccagta cgatttgggg aaaagtatca attcaaaaaa
     2281 atggggatat taaggtaaat atttttccta tcccgtttga ggacttgtta aggcgagaat
     2341 ttcaagtaaa ggatctaacc tatcttttta ggcaattgct tgattcgggt aatatgtgtg
     2401 cagaaaaagg gagaagaacg aaacgtattc acatgaatcg tcaagttctt ccaacatacg
     2461 agattctatt accttcttca ctcaaatcgt attttcgatt gtaatttaga ataaaccaaa
     2521 gtatgaaagt gaggcaccat gttatggaaa tatttcttat tgatttagca aaacaactga
     2581 ttacttatct gtatgatagc gagcaaaccc aaacggaagt aaatgaagct tgatctatgc
     2641 ttcatttact tcattcaatc aaacatgaaa gaaaggaaaa ttctaatgac taaaaataaa
     2701 atgacactaa aagcagaggt tcttctgtat attcaagaac atttttcaaa tcaagccttt
     2761 tttacaaaac caatttactt ggatttcgaa ataagagggg tatctgctgg atctatcggg
     2821 ggtactcttc aagcgttgaa gaacgaagga tatttagaaa atcactttgt gcaacgttcg
     2881 ttcaatagaa gagtcgtgaa aaaatggtat ttagttcatt catagagagg ggaatcaagc
     2941 atgcgtgtgg cttgttatga tcgaacgtct gtttatgaag aagcgaaaat tagtaatgac
     3001 gctcaatatg atgcagtgaa agagtatgtt ttagagaaat atgaacaaat tccccaacga
     3061 ttttcagaca aaggagaatc aggggaaaaa gcggagcgtc cagcttttcg acagcttaga
     3121 gcttgggttt gtgcgtcaga agaagagggg gtgatctatg tttggcgtta tgatcgattg
     3181 gcacgtcaag ccaaaatagc cttagaattt attgagttgt gccaacaaca tcaggttcaa
     3241 gtgtattcga tttctgaacc tcttcctgct tctttctcac acgcgtcaac gaataaacta
     3301 tttgtgcaat tattgttcac ttttgcagaa caacaacgaa acgtcattat tgagaatgtt
     3361 cgtgccggac tttcctataa aaagcaacaa cggcaatatt tgtcttctgc ggtaccatat
     3421 gggtatcgtt tagtgaatgg gaaaatccaa caagaagtac acgaagcaag gattgttcgc
     3481 tatatttttc aactttattt gaccaaaaaa tatggctaca aaaagctttg tcaacgattg
     3541 acgcaacaaa agcttttctt ccgcgaacgt ccttttcagc cctatcatat ttatagtatc
     3601 ctaaaaaatc cattgtacta tggcgaaatc aaaggaggat ctttggggaa gtatctagga
     3661 acctttgaac cgattctctc gaaagcaacc tttttacaag cccaagagat tcgccaatca
     3721 aggtgtacag ctaaaaaaga tacgtatctg tacttattac gtcaaaaaat tcggtgtccc
     3781 ttctgtggtc gtcatctgag ttcgaagtat caatggaata caaagaagac gaagacgctt
     3841 cattattatc attgtaccga ccgaagctgc caaggaattt ttcttcgggc agaaagggtt
     3901 gagtcagaag tattggaaaa gatacaggct ttttttcaat acgaaggcgt ctatcaagcc
     3961 ttgattgccc aattaacgca acaattagcc gaaaagcgaa aagatgaaaa acgcaaagta
     4021 atccaacaaa agaaacaaga agcacaatgg attaaggcat ttgaagaggg caattgttca
     4081 ctggaagaac tcaagcaaca actgcaagct cttcaaaagc cgatgtctac ttcttcgttt
     4141 ctttcactcc aacagtgtga agcaaaagtg aaagaatggc ttcaactaaa agacctctct
     4201 agtcgacaat tattatgtga ctatgtttgt cggattgagg taacaaagga taaaacagtt
     4261 tgtgggattt acttccgaca actgacacaa aatattttag aaaggaacga atgatctatg
     4321 gaaatgatac aaaccaaacg agtcgcattg tatattcgcg tgagtacaca agaacaagct
     4381 ttagaaggac atagtattca agcacaagaa caaaatggac gacagtttgc cgaacggatg
     4441 ggctatgaag tggtagaagt ttatgccgat gaaggaatca gtgggaaatc cacaaaaaat
     4501 cggttcgcct atcaacgaat gatgaacgat gctcggaacc atcgttttga tctactagtg
     4561 atttggaaat tgacccgtct cggacgcaat atgttagatg tcttacaaac agtcgaggaa
     4621 ttattgcgat atgacgtagg attacattcg atctcggaac aatttgacgt gaccacttcg
     4681 acaggtaaat tgatgttaca actccttggg agtttcggtg aatttgaacg caaccaaatt
     4741 gcggagaatg ttcaaatgtc aatgcaaagc ttagtgaaag ataaaaagcg ttatgcagga
     4801 ggccgacgtt taggctatgt cagtggtatt gacacgaatg gtcaaaaaca attggtcatt
     4861 gaaccagaag aggcgaaaat tgttcgtctg atctatgcaa aatatttagg cggagcaggg
     4921 tatcgagcga ttgccaattt tttgaatcgt caaggatatc aaacagtcaa aaagaatgct
     4981 ttttctacca cggcagtcaa agatatctta cgcaataaaa tttatgcagg gtatttagaa
     5041 tatgcgcgct acatcaattg ggaaaccaaa cgacggaagg ggaagaatcc gagaccgatt
     5101 ttagtgaaag gaacccatga accgattatt tcggaagaag attatcaaaa agtacaagaa
     5161 cgccttgctt tagaaggaaa acaacccaaa tggaaccata caggtgaaaa tgtattgaca
     5221 ggcttgttac gttgtccaga atgtggcgca ccgatggcag cgagtaatgt cacaaatacc
     5281 ctaaaggatg gcacaaagaa acggattcga tattattctt gttcagtctt tcggaataaa
     5341 ggagcaagtg tgtgtcatgc caattcgatt cgtgccgaac aagcggaaac atttgtcgcc
     5401 gaccgattaa aggagatcgt tcagctacca gaagttcttc cccgattggt ggcagcgtta
     5461 aatgaagaaa tagtgcgaca aagccaacca ttggaacaag aactcgtggt gttactggaa
     5521 cgaaaggaag aattgaagac taaaattgaa aaatgggaag ctgctttgga agatagtcct
     5581 gaattgtttc cgatgttaaa agaccgctta gacgaattga ctgaaaaacg acgacagttg
     5641 cacattcgag aaaatgaaat cttagggatc tttcaacaag gagagccgat tcaagtcaaa
     5701 gatgtccaac ggatattgac tagtttggat cgttttcttg cccagagtga gaagaagcaa
     5761 atcaaagcct tgtatcgtac ctttatagag aaaataacct ttgatcctaa ccataaggag
     5821 atgttggaaa ttacgatgaa attcacgcat gccgtggtac aacaattgaa tgagcaatat
     5881 caaatagccg tatccaaaac aaaggatacg gctatttttg tgttaaaaac accctttacg
     5941 ttaacgatct agtctaggtt atatcaaaca acgttagagg ggcgctaacg cttctctagc
     6001 gatttgaaat cgttagagaa gtagttaggg aggtccttat cataaatagg gatatttatt
     6061 aatgaatatt tgaaatataa ttgtatataa gaattaaaaa gagtggggat agatatgaaa
     6121 aagatgggta agtgtatttt atgtgaaaaa tacactgaac ttactaaaga acatgtacca
     6181 cctaaaaaat cagggaatac tggaggtaaa aagaccagaa ctggtaattt agatgatttc
     6241 cttaagagcg attttacaaa aggtgatttt ccgaagggga ttaaaaggaa acctcaagga
     6301 aatgtttatt ata
//
LOCUS       DAYYCW010000129         6303 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000129 DAYYCW010000000
VERSION     DAYYCW010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.131"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            334..714
                     /locus_tag="ACHAYA_002623"
     CDS             334..714
                     /locus_tag="ACHAYA_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM8606367.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="ACHAYA_002624"
     CDS             696..1610
                     /locus_tag="ACHAYA_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8606368.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="ACHAYA_002625"
     CDS             1855..2544
                     /locus_tag="ACHAYA_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606369.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="ACHAYA_002626"
     CDS             complement(2665..2751)
                     /locus_tag="ACHAYA_002626"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8606370.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="ACHAYA_002627"
     CDS             3427..4377
                     /locus_tag="ACHAYA_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8606371.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="ACHAYA_002628"
     CDS             4398..4931
                     /locus_tag="ACHAYA_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8606372.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="ACHAYA_002629"
     CDS             4947..5117
                     /locus_tag="ACHAYA_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606373.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="ACHAYA_002630"
     CDS             5567..6100
                     /locus_tag="ACHAYA_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606374.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            6112..>6303
                     /locus_tag="ACHAYA_002631"
     CDS             6112..>6303
                     /locus_tag="ACHAYA_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606375.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKS"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DAYYCW010000130         6278 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000130 DAYYCW010000000
VERSION     DAYYCW010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.132"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..796
                     /locus_tag="ACHAYA_002632"
     CDS             <1..796
                     /locus_tag="ACHAYA_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8606376.1"
                     /translation="FESEGFDIVIHLAAQAGVRYSIENPYAYIDSNIIGFLNILEACR
                     NFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKKSNELMAHTYSHLYKIP
                     TTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKMERDFTYIDDIVEGITRL
                     INKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFVETIEKYLGVKANKIFL
                     DMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTVYYLS"
     gene            1164..2588
                     /locus_tag="ACHAYA_002633"
     CDS             1164..2588
                     /locus_tag="ACHAYA_002633"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFM8606377.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDA
                     VITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNPDYKKSLVLVPILV
                     IMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGYSFGVIGVSFATLL
                     GTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGFYFNLFLTIIFSAI
                     FVFTYKQLIITYLKKFFRIKMAKR"
     gene            2674..3102
                     /locus_tag="ACHAYA_002634"
     CDS             2674..3102
                     /locus_tag="ACHAYA_002634"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFM8606378.1"
                     /translation="MIFGKKHSLVEVPEFGSEPYLIEIGDNFRCSFGVKFITHDGGVW
                     VLRKMSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDNVIIRANTLVNKDIPNNS
                     VVAGSSCKVISTIEEHYEKNKNEFIKYRTSMILKQKNFFY"
     gene            3190..4356
                     /locus_tag="ACHAYA_002635"
     CDS             3190..4356
                     /locus_tag="ACHAYA_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFM8606379.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
     gene            5572..5691
                     /locus_tag="ACHAYA_002636"
                     /pseudo
     CDS             5572..5691
                     /locus_tag="ACHAYA_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
     gene            complement(5739..6005)
                     /locus_tag="ACHAYA_002637"
                     /pseudo
     CDS             complement(5739..6005)
                     /locus_tag="ACHAYA_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6032..>6278)
                     /locus_tag="ACHAYA_002638"
     CDS             complement(6032..>6278)
                     /locus_tag="ACHAYA_002638"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606380.1"
                     /translation="KKYRKDTLKSFRINLPNKFIGGFKMSHTLLIKDTLNILDLNINI
                     HFEENCLTKEKYKGQICLIYKGTLQYTPEEYPHLPIY"
BASE COUNT         2074 a          844 c         1174 g         2186 t
ORIGIN      
        1 atttgaaagt gaaggttttg acatagtaat tcatttagct gctcaagcag gtgttagata
       61 ttctattgaa aacccttatg cttatattga ttctaatatt atcggttttc ttaatattct
      121 tgaagcctgc cgaaacttcc ctgtaaagca tctattatat gcatcatcta gttcagtata
      181 tggtgggaac acgaaagtac cgttttcaac agaagattcg gtggatcatc cagttagcct
      241 ttatgcggct accaaaaaat cgaatgaatt aatggctcat acttatagcc acctttataa
      301 aataccaaca actgggctga gatttttcac tgtctatggt ccatttgggc gaccagatat
      361 ggcctatttc tcttttacca agaatatttt atctggaaaa gaaattaaga tttttaacca
      421 tggaaagatg gaaagagatt tcacttacat cgatgatatc gtagagggaa tcactagatt
      481 aatcaataaa gtacctgaac ccaactccga ctggacagac atcgatgatt tgagtagtga
      541 aagttgggca ccatataaga tatacaatat tggaaataat aagccggttg cattgttgga
      601 gtttgttgaa acaattgaaa agtatttagg tgttaaagcg aacaagattt ttttggacat
      661 gcagccgggt gatgtcgtta gaacttatgc cgatattgaa gatttagagg aaaaaatcaa
      721 ctttaaaccg aatataacct tggatgaggg cattaaaaaa ttcgttgaat ggtatacagt
      781 gtattatttg agttaatttt gaaaggaata ttgaagatgg gtaattcata cgattttttt
      841 agctttgtta ggtattttag gaaattctac tttcaaacgt gacgaacttt ctcgccgaat
      901 agatattttc tataactaaa agaatttcta taaaacaaaa ttgtgtatca agatgtgtga
      961 actggattct gactgccaat attgattgat gagattgaag aaatatatag ttgatcatgg
     1021 atatctttat ttgaaaagat cttagctatt cacatattca aaaaaatttt tactacgagg
     1081 actcacacga tacagaggaa tttaggtagt tctaattaaa ccagaaagtc atctatagat
     1141 gtgagttgag ggaggtcata cggttggata gtagcagtaa taaatcacat tcaatagcta
     1201 aaaaagcttt tatttacgca ataggaacat tatcatcaaa aatgatatct gtcattataa
     1261 tccctatcta tgcagtatat atttctgttg ccgatttagg aagctttgat tatcaaaata
     1321 caattgctca atttctttct ccagttattt ttggagcaat atgggaagca attttacgca
     1381 tgggattagg caaagaaaaa aatattcaga aaaaagtagt gacgaacagt atttgttttg
     1441 ttatagcaaa tacagtgata ttagccattt ttttaccaat aatatataga atgattttcc
     1501 cgaatttaga gtattatcaa ttgtttgttt ttcaattaat tttcactcct atcctaagtg
     1561 tttttcaata tattgtaaga gcactaggtg aatcaaagat ttttgtgatg agtggtataa
     1621 tcgctgcggg tatcaacctg ttactcctta ctatttttgt tgttacaatg ggtatgggga
     1681 tacttggtct cttactctct atcataattt ctaatgttat aaatttgatt tttttgattt
     1741 tcagggggca gatctttcaa tatttcgcaa cgagttcttt tgatttttta tttttaaagt
     1801 ccatgctgaa atactctatc ccactcactt tgaatctcac ttttggatgg ctaatagttg
     1861 gtttttcaag attttatatt ggaacgtaca tcggagtaac agaaaatggt attttttctt
     1921 ttgcgatgaa attttcatct ataattttaa ctcttacaag tattgttagt atgtctatga
     1981 tagaggatgc ggttatcacg tctgagaatg atgatttcat cgaaagattc gttgagaaaa
     2041 atacctatat ttttaaaatg tacattaggt tagcgactct ttctattcct atcatttgct
     2101 gtgcatactc ttttataacc aaccctgatt ataaaaaatc attagttcta gttccaatat
     2161 tagttattat ggcatgtttc agtgggatga caacaaatat cggaaatata tttgcagtat
     2221 acaataaaac aaatcagtta ttaatgattt cagtagtgtc aggtctattg agtgttgttg
     2281 gcacctattt attaggctat tcttttggag taattggtgt ctcatttgct actttgctcg
     2341 gtactttagt attatttgga cttagatatt attttggaag gaaaattgct tattatgcat
     2401 ttgattggaa gcgaatatcg ctacttttag ttctctattc cgcaatcgct tttgtatgca
     2461 ttcatagagg cttctatttt aatcttttct taacgattat ttttagtgct attttcgttt
     2521 ttacgtataa acaattaatt ataacgtatc taaaaaaatt tttcagaata aaaatggcta
     2581 aaaggtgagg gtatacgaag taagaataga atctttcgaa gaataaagag aaaactattt
     2641 ggaaaaaatc tgcgagaatg tattaatgaa ggtttgatat tcggaaaaaa acatagttta
     2701 gtagaggttc ctgaatttgg gagcgagcca tatttaattg aaatagggga taatttcaga
     2761 tgttcatttg gtgttaaatt tataacacac gatggtggag tctgggtctt aaggaagatg
     2821 agttctgtga gcgagtcaac ttcaaaaata ggtaggatca aattgggaaa taatattttt
     2881 attggtattt actctattgt tttcccagga gtttcaattg gcgataatgt aattattaga
     2941 gcgaacactt tagtaaataa agatatccca aataatagtg ttgtagcggg tagttcatgt
     3001 aaagtgattt caactataga ggagcattat gagaaaaata aaaatgaatt tattaaatat
     3061 cgaacctcga tgattttgaa gcaaaaaaat tttttctatt aaataatatt gattgaataa
     3121 ggaatatgtt caaaaggata acagctaaaa tactataaaa tgaaatttga ttggagcgta
     3181 tggaagaata tgaaaatttc agttttcgga ttaggctatg ttggtctagc aaattcacta
     3241 ttattagcac aaaaagagca ggtcaaagct tatgatatcg tggaagaaaa aatcacgatg
     3301 ttacaaaatg gcatctcacc aatagaagat aaagaagttc atgaattttt aaaacgggat
     3361 gacttaaatg tcgaatttac gtcaaatttt actgatgcag taaacttcgg tgattacttg
     3421 attattgcaa ctccaacaga ttatgacgaa aagaaaaact attttaatac atcaacagta
     3481 gaagatgtaa ttgagaaggc tttagcaatt cgtccagatt caacattcat tattaagtcg
     3541 acagttccag ttggctatgc aaatgaaatt aaagcgaaat ttggtacaga aaacatcatt
     3601 ttttcacctg aatttttgcg tgaaggaaaa gctttgtacg acaacttata tccatcaaga
     3661 attatagttg gagaatgttc tgctcgtggt cgcgagattg caactatgtt tcaaagaaac
     3721 gcatttaaca aagatgcatc agttcttttg actcactcaa ctgaagcaga agctattaaa
     3781 ttattctcga atacatattt ggcattgcga gtggcgtatt tcaatgaatt agatacgtat
     3841 gcagaatttc gtgggcttga tagtaagcaa attattgaag gtgtcggttt ggatccacgg
     3901 atcggttcgc attataataa tccttctttt ggctatggtg gatactgttt acccaaagac
     3961 acaaaacaat taagagccaa ctatgaaaaa gtaccaagca atatcatcag tgcaattgtt
     4021 gatgcaaata caacaaggaa agattttatt gctggacaga ttttggcgaa aaaaccaaat
     4081 gttgttggaa tctatcgttt aacgatgaag gccggttcag ataattttcg atattcgtcg
     4141 atccaaggaa ttatgaaacg gattaaagcc aaaggaattg aagtggttgt ttacgagcct
     4201 acattggatg aagatgaatt ttatcattca caagtattga aagatattaa tgaatttaaa
     4261 aatatttctg atgtgatcgt atctaatcgg tttgaagata ttttaaagga tgttgaagaa
     4321 aaagtttata ccagagatat ctttggtgat aactaaataa taatgaacgg gtacatggag
     4381 gaaaatattt tagatggtga ctcgaaataa atgtttgttc tcattgcatc tgtatctata
     4441 ccgataagtt taaaaattcc gattaggaaa atgaagttgg ctatccttgc gaggaattta
     4501 ttgctggatt attttatgaa ctaaagcaag acatcatcag aaaaaaatcc aaaatttgac
     4561 aatgggattg atcgtttacc agctgtaagc gaagtatctg acacgaaaat ggaaaatgca
     4621 attactgtta ttaccaatag ctttttttca tcgatcaagt atttgaaaac attatttgtt
     4681 tgccaagctg atcaatgttc tttcagactt ggaagaacat tgatcagctc acaagtaatg
     4741 attgaagttt aggttatggg ataaaggata agtgaaataa aagtcgtgtc tgcatctttg
     4801 ttagtaccag taaatttttc tactacggcg ctagtcacga cagtccaggc atctgaaatt
     4861 gaagatgcaa atagagtttc tgctagttta ggtattgatg ttactgtatt agcattaatc
     4921 ttagagccag tagcatccaa taggtgtagc tatagttcta atagtataaa acaatgcaga
     4981 ggatatgatt gctttatgtc tcggtgtgtc aggtcaaaat agagttccat atggggcgat
     5041 ttctcaatga ataatcttat agatataatc ttgtttgcta tttcgttatc acttttactt
     5101 ttcttcgatt atttcaaaaa taaaaatcca aaatatttta agttcttact atttatcatt
     5161 actgtactag ctgttttatc gatgacagat tttatttaac ttatctcaaa aagtagggaa
     5221 ccatatcatt tttcagtaac atgcgtatta tttgtataaa taatacaaaa tgaaaaatta
     5281 tgattattct attgtctttt tagaaagcgt tttctgtgag cttttaatct aaaaggaggg
     5341 atataaaata aagaaaacct attttatgct atttttttgt tgtttctgtg taggaatcct
     5401 gtggtatata ggtggtaatg aaaagaagat tcatggagtt gtgacaaata tttctgcgga
     5461 tgagttcgta tttgaagatg aaaaacagaa cgaatatagt attcaaaaaa gaagctcttt
     5521 gtcaaatgat gttggtaaag agaaatctta gaataatttg gacagaagag gctgggacag
     5581 aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa ttgcttttca aatttttgtg
     5641 aatttcggct tatttccgaa ggagttgctt ctgctcccgc cgtttactcg agagtctgta
     5701 gcaaaactga tttttagatt tatcccacat tcttctgttt atttggttaa aaatgttttg
     5761 ccatgacgaa tcatgatccg tagtttgaaa ttgtaaaagc tgcgaaagcc atacgtattg
     5821 cgttttaata ctttgatgtg gttgtttaaa cattccaatg gtctgtttga atagctgcag
     5881 ttaagcgcat tttgaatctg tggcagaaaa tttttaaacg ttcgtaaagt ggttgtatag
     5941 gtttcaggca gatcgctaat gtctctagtt aaacagtcct caaatagtga cgaatctctt
     6001 ttctttcact caatgattca agaaggttct tttaatatat tggaagatgt ggatattctt
     6061 ctggtgtgta ctgaagagtc cctttgtaaa tcaagcaaat ttgtcctttg tatttttctt
     6121 tagttaaaca attttcctcg aaatggatat tgatatttag gtctagaata tttagggtat
     6181 ctttgataag cagagtatga gacatcttga atcctcctat gaatttgttg ggcaaattaa
     6241 ttctaaagga tttcagggtg tcttttctat attttttt
//
LOCUS       DAYYCW010000131         6224 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000131 DAYYCW010000000
VERSION     DAYYCW010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.133"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(207..1169)
                     /locus_tag="ACHAYA_002639"
     CDS             complement(207..1169)
                     /locus_tag="ACHAYA_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8606381.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="ACHAYA_002640"
     CDS             complement(1171..2610)
                     /locus_tag="ACHAYA_002640"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM8606382.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="ACHAYA_002641"
     CDS             2795..4741
                     /locus_tag="ACHAYA_002641"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM8606383.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /gene="scrK"
                     /locus_tag="ACHAYA_002642"
     CDS             5005..5877
                     /gene="scrK"
                     /locus_tag="ACHAYA_002642"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8606384.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1986 a         1061 c         1107 g         2070 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaggc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa ccgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat
     6121 aggttctgtt gcaaagtttt aaatctacta tcagataagg tagaataata gaaaaagata
     6181 gcaggaggaa tgacgatgag tcattttaaa ggaaagcaat ttca
//
LOCUS       DAYYCW010000132         6173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000132 DAYYCW010000000
VERSION     DAYYCW010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.134"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACHAYA_002643"
     CDS             725..811
                     /locus_tag="ACHAYA_002643"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8606385.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACHAYA_002644"
     CDS             complement(932..1621)
                     /locus_tag="ACHAYA_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606386.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="ACHAYA_002645"
     CDS             complement(1866..2780)
                     /locus_tag="ACHAYA_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8606387.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACHAYA_002646"
     CDS             complement(2762..3142)
                     /locus_tag="ACHAYA_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM8606388.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACHAYA_002647"
     CDS             complement(3592..3879)
                     /locus_tag="ACHAYA_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8606389.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACHAYA_002648"
     CDS             complement(3899..4123)
                     /locus_tag="ACHAYA_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8606390.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACHAYA_002649"
     CDS             complement(4578..4748)
                     /locus_tag="ACHAYA_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606391.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACHAYA_002650"
     CDS             complement(4763..5296)
                     /locus_tag="ACHAYA_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8606392.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACHAYA_002651"
     CDS             complement(5317..>6173)
                     /locus_tag="ACHAYA_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8606393.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAYYCW010000133         6060 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000133 DAYYCW010000000
VERSION     DAYYCW010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.135"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..1181
                     /locus_tag="ACHAYA_002652"
     CDS             153..1181
                     /locus_tag="ACHAYA_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606394.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(1207..1647)
                     /locus_tag="ACHAYA_002653"
     CDS             complement(1207..1647)
                     /locus_tag="ACHAYA_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8606395.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            1807..2313
                     /locus_tag="ACHAYA_002654"
     CDS             1807..2313
                     /locus_tag="ACHAYA_002654"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8606396.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2481..4394
                     /locus_tag="ACHAYA_002655"
     CDS             2481..4394
                     /locus_tag="ACHAYA_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8606397.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            4399..5400
                     /locus_tag="ACHAYA_002656"
     CDS             4399..5400
                     /locus_tag="ACHAYA_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8606398.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5602..5808)
                     /locus_tag="ACHAYA_002657"
     CDS             complement(5602..5808)
                     /locus_tag="ACHAYA_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM8606399.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2241 a          925 c         1207 g         1687 t
ORIGIN      
        1 cttagtcaaa aaattatata agtttgcgta cgattcaaag aaaattaaga gaacttaata
       61 ctattttgaa aaactatcat ttatcttgca gcttgaaaaa aggtaatccg atagaaggag
      121 aagaataccg tatccgatac ttttttcact tgatgtattg gcagtcattc gacgaaaatc
      181 attctgatat attatcaagt ataaaaaata aacgtcaact attagaaaaa tttgcgaaac
      241 atgcgccata tacgcgtagt atagatagaa agaaatggat cagcctacta agaatttcgt
      301 tgcaacgaat gaaacaaggc cactcgatta cgtatgtccc agaagagatg aacaaatttg
      361 tgcatccatt gataacaaaa gaagagttta cgcattacat actagagcct ttttttgaag
      421 caaacttctg gtcgatgaat cacatcactc caacagagca agcttacctt tattttatgt
      481 ttggtgtaat gaacacctat ttggaagaag atttgttgga tactccagat aaagtacaag
      541 aaagttgggg ctggattgaa tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt
      601 gttttacaac ttcagaaaaa aattatctat atgtcaattt ggcaatgatc catttgtatt
      661 caagggtttt tgggacaaaa aagaaggtgg atgcttttgg aaaatcagca gaagacagag
      721 attttcagca tatatttcca gtgatgtatc caatcatgca acagtttttt agtggggtag
      781 ctcgcagaat tccagagctt aatacttatt atgaaaagaa cagacggttg atttttcaat
      841 actgtatgtt agtcagggat atttttataa aacacgaaca acctgtcata ttctatattc
      901 aatcaaagta tggtaaaaca caggaattat gggcaaagaa aaggatatta tctatgactg
      961 aacgcccaat aaaatatagt gcatccgctg atcagttacc tgatattgtc attagcgatt
     1021 atcctatcga caaaaaggca tatgatcaaa cgaatacaca gatattttac tggaatggac
     1081 aacctacaaa aaatgattgg gcgcgtctga aagctgaaat cataaaaatc aggaatgaga
     1141 aaaatagatc catattggaa aaattggatt tcgttaatta agaaaagcag attgtttatg
     1201 ccaaaattaa gacattgcaa aaagattatt ttctttaaaa tcgacaagat gaccagaaag
     1261 caagagttcg ttgagctctt gctttctttt agttttagaa aggaacatcc tgattttatc
     1321 ttcgctaaat ccagtcaaaa gttttgcaga ttggtcgtag ataatcggtt ttttcaataa
     1381 agaaggggat gtacgaatct caatatatag ttcactcaaa ctgagttcat ctattctgtg
     1441 aacaagattt ttataactaa tagaatgagt agagagcatt tcggtcacgc cgttgtttgt
     1501 caattttagt aagtggataa attcattttt ggttagtggt tgacgaatta agttctgttc
     1561 gataaacggt atatcatgga cgataagcca acgttttgcg cgtcttgaag acgagcagcc
     1621 agaacctgta tacaatttaa tcatcataca ttcctccaat agctaagtat ttatgaaata
     1681 ataacatgaa gaaatagtcg aaaagtgcca tttagaggtg taaaataaga aaatgttgtc
     1741 acgtataatg gaactagcta gataagaaat actattaaat caagaaaaaa gaaagaaggc
     1801 acaagtatgt caaacacagc cgacgcaata gtagtgatcg atttacaaaa aggggtatgt
     1861 tttgattctt cgccgataca cgaattagaa acagtgacag cattagtgaa taaaagaatc
     1921 aaaacatacg aaacgattgg gaaaccagtt attttcgttc aacacgaaga tgctcaatta
     1981 gtgccacaaa caaaaccatg ggagctattg tcggaattat ctattcctga gcacagttat
     2041 tttgtgagaa aaacccatgc gaattcattt tataagacta atttgagtgt tttattaact
     2101 gaattggact gtcattcgat cgaattatgt ggggcacaag cagagtattg tatagatacg
     2161 actgtgaagt ttgctcatgg attaggatac cagcttatga tgtgtagagg agcaacgaca
     2221 acttatgata atccttttat gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg
     2281 gataaacgat ttctgacatt tttggagcaa taagttgttt cttcgtttaa aaaaagcaga
     2341 atggatactg gtttatccat tctgctttat tacgtaaaat gagaaaataa tcctgttcta
     2401 tttttatggg ataaaccatt tattttttgt ataaaaaatg tttgcgttta catataattc
     2461 tcctagaaac ataaaaagga gtgaatagct tgagaacaaa acaagtatta aagggagcgt
     2521 tggtcatctt atttgctgta agtacaagcg tcccgcaatg gacagtatat ggagagagca
     2581 tttcttccga atcggaatct cgggaggtag aaaccacaga aactactgat gctggaaatg
     2641 aaacgaaatc gagtacttca caaacagaaa caaatactga aacaacggtt gagacatctg
     2701 ttactagcac agatcaagaa acgaccgcta ccacagataa ccaacaaaca gaagaagcaa
     2761 cagctacaaa aatgtcaaac gacgaatcct cttctgctaa agtaagtgac aaagagaatg
     2821 ggacagaaaa tgatgatatc ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt
     2881 acttcggtaa acagatgaca gaaatactta atggaccaac agaagacatc aagtcaaaat
     2941 taatagagtt attagtcact gagcttgctg ttaatgatta tggtgtagat aaagagatta
     3001 tcaaacatct gattgctact tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt
     3061 tgatgtcgat gcttggaggt acagatgatg ggacaacgcc tacagaaaac acaggcttct
     3121 atccaaatac aaaacaactt catcaaactg tactaaacaa acaagatggc aaaatgatgg
     3181 atgtatatgc atattatgtc gatcaaggtt cagataaaac agtcatgatc catggcggat
     3241 ttcgagggaa ttggaataac gggatcgtta cagaagaata caatgatttt tataaggccg
     3301 ggtataattt attatttgtt gattccagag cgactggtaa tagcggaggc gattatgtga
     3361 cctatggaca atatgagtca gatgacgtcc tttactggat aaatcaagaa gttcgagagc
     3421 ggccatctca aaagatttta ctttatggtg gttcgatggg agcagctacg atgatgtctg
     3481 tattagcgaa agatattcct gtcaatgtca aaggaattat tgaaaattgc ggatttgcat
     3541 caattgatga acaactacgt tttacgtaca gccagacagt agcccccgca ctaccggatg
     3601 ccataaaaaa tcagttagac atcattgggg atcaagagca tgaagattta tttatgggcc
     3661 ttttgaaaca atactatttt gatcaggaga tgcatttgga tacaaaagct gctctaccta
     3721 cgattggaat gtcagattca ttgcctaaat tgattatcca tggaacagca gatgatgtag
     3781 tacctgtgtc taacgcgcaa aaattatatg agttatcagg agggtataaa gatcttttat
     3841 tagtagaagg agcagggcat ggaaaggcac aggaagttga ccatgctgcc tacacgaaac
     3901 atgtaacgga ctttttgaaa gtagtgttcc atgatcagat aaacgtaaag tatgtggatg
     3961 aaaacaataa aagtctactc aaagaccagg acgaaatcca gctttacgga gcttatggag
     4021 aaaattatat gacagagcaa aagacattcg aaggatacga attagcaaat gttgaaggac
     4081 cgactgaagg gatttttaat gagacgatac caactattat tttctattac aaaaaaatcc
     4141 cagtagctcc accaaaaaag caagatccga cagagaatgc agacaataag ggaaagaatg
     4201 aacctcgaga ggatcaatca agtgatgata cgcaaccgat tcagatgaca gtgaaagtac
     4261 aaaaaaagac agatgcaaaa aaaagaatgt tgccgaaaac tggagaaaag aaacattcgc
     4321 ttctcattta ttcaggtgga tttttatctg ttagcacgat tatcacatgg atttggcgaa
     4381 gaaagagagg gtaaatgaat gaaatggttc aagcgaatag tgattgcagt accagttatc
     4441 atcgtagtta ttttagtcac tgtatttttg attaatcaaa tcacaccaaa gccgattagc
     4501 ttgcttgtta gaaaacaatt tgacacaagt gaaaaagaac aagtatatgc ccgaccagat
     4561 gatttccagc aaaaaatcga acaaatcgat ataaaaaaag aaatatccta tccttcaaag
     4621 tacaaaaata ataatatgga tatctacaca ccgaaagtgg aaaagaaaaa attaccgatt
     4681 cttttctgga tgcatggagg cgcatatgta ggaggggata aaaatgattg tcgtgattat
     4741 ttggaatatc tatgttcaga cacgcagcaa atcatcgtaa atatagatta tgaacgatca
     4801 ccggaagcaa aacatcctac accagttatc caattgaacg aagccattca agcagctaaa
     4861 aaagagataa aagatcaagc agattggtcg aatatttcga ttggtggaaa ttcggcaggt
     4921 gcacaaatta gtggagaata tttgttagca cttcaaaatg aagaaattaa aaaagctgat
     4981 catcttgatc cgactttgga gaacaagcag attacgaagt ttatttcttt gtctggtctg
     5041 ttggatcctt ctgcacttac tcaagtaagt gataaaatct ctagctttct ttatgaaaaa
     5101 tgtggatggg cttactttga taataaggac tttgaacaat cagagaaggt caaaagttta
     5161 gccttggtca gacatgcgga tcgttggacg caaaacacat tcttaacgga tggaaataca
     5221 aatacgttta cgaaacaaat ggaagaagta acttctgtat ttgagaaagc gggggtgaaa
     5281 gtcacaaaag tagattatgc ggagaaaaat gctgttttga accatgaata tcagtttgat
     5341 ttcagcaata aacaagcgca agaaacatat caaaagttag taatgttttt caaggagtaa
     5401 agtcttatga tgcaaaagag gttgtaatct gtcacttagc tccaagtcac aaggagcaat
     5461 ttcaataaac aagcttttca tggtttatga atattgtaac ttgtgactga ggagttagag
     5521 atggcaaggg gcacctttta ttcgaaattt gggaatatca cacgattatt gtcacgatct
     5581 cttttacgta aattttataa gtcagatttg ttgttttgat cgtttaaaaa aagcaacaaa
     5641 tccgaagcct gcggaggcaa gaagggacaa aaagccgagt ggtaagcaaa agaatgctgg
     5701 ttccactaat attccgttgt tattgatata aggctgggaa caaagatgaa tagcaaataa
     5761 aatcaatgca ctagcgaaaa aaagcaaaga aatatggata gctttcataa gcagcacctc
     5821 cttttttata attgtatgat aaatagatta agaatctata aatgtgacaa gtttgttagg
     5881 atgtgaataa aatgatcatc tagcgaactt gaaacaaaga tggctaatga taagcaatta
     5941 atgattcagc aatcagatag gtacaaagtt taccatcata cgtctagacc aataatagaa
     6001 aatatgggaa tataatagaa aagatttagg atacccccaa aagaatttcc ccaaaaattc
//
LOCUS       DAYYCW010000134         5781 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000134 DAYYCW010000000
VERSION     DAYYCW010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.136"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..465)
                     /locus_tag="ACHAYA_002658"
     CDS             complement(7..465)
                     /locus_tag="ACHAYA_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8606400.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(478..1809)
                     /locus_tag="ACHAYA_002659"
     CDS             complement(478..1809)
                     /locus_tag="ACHAYA_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM8606401.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1820..3262)
                     /locus_tag="ACHAYA_002660"
     CDS             complement(1820..3262)
                     /locus_tag="ACHAYA_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8606402.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3249..4013)
                     /locus_tag="ACHAYA_002661"
     CDS             complement(3249..4013)
                     /locus_tag="ACHAYA_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM8606403.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4117..4992)
                     /locus_tag="ACHAYA_002662"
     CDS             complement(4117..4992)
                     /locus_tag="ACHAYA_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8606404.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5247..5331)
                     /locus_tag="ACHAYA_002663"
     tRNA            complement(5247..5331)
                     /locus_tag="ACHAYA_002663"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5295..5297),aa:Leu,seq:caa)
     gene            complement(5349..5420)
                     /locus_tag="ACHAYA_002664"
     tRNA            complement(5349..5420)
                     /locus_tag="ACHAYA_002664"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5386..5388),aa:Gln,seq:ttg)
     gene            complement(5430..5502)
                     /locus_tag="ACHAYA_002665"
     tRNA            complement(5430..5502)
                     /locus_tag="ACHAYA_002665"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5467..5469),aa:His,seq:gtg)
     gene            complement(5564..5648)
                     /locus_tag="ACHAYA_002666"
     tRNA            complement(5564..5648)
                     /locus_tag="ACHAYA_002666"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5612..5614),aa:Leu,seq:caa)
     gene            complement(5669..5739)
                     /locus_tag="ACHAYA_002667"
     tRNA            complement(5669..5739)
                     /locus_tag="ACHAYA_002667"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5705..5707),aa:Cys,seq:gca)
BASE COUNT         1752 a         1237 c          891 g         1901 t
ORIGIN      
        1 cgtacactaa aattcaaaac aactatcttt aatctttact tcttggtttt gttttaataa
       61 atttccttca aaaatgcctg gagaaactac aatgatcacc gtatcttttt gtgcttgctc
      121 caacttcttc aaatctataa tagctaacag tgtcccttct tttacctcct ggttactttc
      181 aacccgtatc tgaaacggac ttccttcgag ttcaactgta tctaaaccca tatggatcag
      241 tatttttctg ccagattcac tttcgatcgt tatggcatgt cgggtaggaa atatattatc
      301 gattattcct ttaactggcg catggatata tccatcatga ttcgttatag cgtatccaac
      361 tcctatcatt tttgaagaaa agacctcgtc ttttactgct gttaatggaa taatttgacc
      421 actagctaca gcttttagaa tgtttcttgt tttaaagaaa tgcacttttt atcacctcta
      481 ctttaatgct ggccaatatt ttttgtttac tttgatcatt tcatctagta ttttttttgc
      541 gactctggca tttggtacgg tttgattcaa tgtaaatgct tgtaaagctt tttgataaga
      601 accttcaaag taagcatcta ctagtaactt ctctactgca acttgtgcct ccatcaatcc
      661 tttatgaaaa tcactgattg gttttcttag tgtaattggc tccacaccag ttgcaccaac
      721 atatgccggt atttcaacaa ctgcatctga ccgtagattc ggaatcgccc cttgattagg
      781 aacgattagc ataaagcgac gacgatcatc attcaacaag gaaatagcta tatcgacgat
      841 atattgtcca tgctccccaa aattaaagtt caatacttct tccacaatac cgcggcgtat
      901 tttatcagcc atttctttgg tatttttctc tcgtccatcc atgaccatat tagcacgtgt
      961 atactctttg tcagtatgct caacgaccat atcaggatat aagtaatact ctaagtaatt
     1021 attcgggata ttacttggaa aatttttcgt gatgactgac atcatttgaa aggttttttg
     1081 ccaagtttta tctcctatat taaaatcggc gacttgcatt tcttttgtta ttagtttttc
     1141 aataatctct ggcataattt ctcgttgcaa ttctttatca taaatcgatg tataccagcc
     1201 aaagtggttt aaaccataat acgtaacgat ccaattctta cgatcataac cgtaattcac
     1261 tgcaatcgtt tcttcaattg aaatagtcat atcacaggca ttaatcattt ttacattcgg
     1321 atatttacga cgaacagctt cagaaacgat cgactctgga tttgtataat tcaaaatcca
     1381 tgctttgggg gcatagtctt gtatatgatc gacaatttcc aataaacctt tgattgagcg
     1441 catcccataa gcaaatcctc cgagtccaca cgtttcttgt cctactaatc catgtttaag
     1501 tggaatcttt tcgtcctttt ctcgcatctc caatcctcca acacgaattt gtgagaaaac
     1561 aaaatcgcat cctgtaaaag caagctttgg atcatccgtt gcacgaatgc gaatatttga
     1621 aaaacctttt cttttcagca taaatttaac aatcaagtac atatccatat ttctttcttc
     1681 atcaatatca tataaacgta tttcagataa tggcaaccgt ttttcattgt tcaaaatggc
     1741 ttgaacgatt cctggtgtat acgtactccc gcctccagct atagtgataa tctgtttttt
     1801 catcatcatc ttttcctttc tattcttcta ataatttatt gaattctgcc ataacataag
     1861 tcacttctaa gccgatgata atttgataat ttttttcagt tggtttaatg atcccgctta
     1921 tccctaattg cttgattcgc tgttcctcga ccatggtggg atccactacc tctacccgta
     1981 ggcgtgtatt acaactatag acatccagga tattggcttt tcctccaaac gcttgtagca
     2041 tttttgttgc catatattca taatttttac ttgctaaccc taactctgtt tcttctaagg
     2101 ttacttcatt gccaaactct gccttagctt cacgtcctaa tgtctgaatg tttctcttat
     2161 tgatcaaata ataaaaagta acgtaataca aggcaaaaaa gaaaattccg attggaagaa
     2221 tcaaaatagc attggtagcg attcgaaaat tgatgagata atcaacaata catgctccga
     2281 atgtaaaacc aattcgtata ttgaataagt aacagacaat ccctgctaaa ccggcaaaaa
     2341 gtgcatggat cacgtacaac ataggagaaa gaaacatgaa actaaattca acaggctcag
     2401 tgatatttgc aataaatgaa gttcctgcac cgcttcccat gattcctttg accatctctt
     2461 tattttcttt ataagctgct ttatagatag ctagacatat agctggtata ccaaacatca
     2521 ttactacaaa aaaaactgat aaaaacagtc cagcctgagg atctccgttg ataaagcgtg
     2581 tcatctctcc tctgaaaact tccttgctag gagatgtata gcttcctaaa tcgtaataaa
     2641 tatacgtatt caacacatgg tgcagtccaa aggggatcag caaacgattc aaaaacataa
     2701 atatccccac acctaatgct cccatatcaa cgagcagttt tgcaaaatta tcaatcccaa
     2761 tttggatgag cggccagata aagcccaaaa aaacagctgc gattgtttgt agaatcatca
     2821 ccatagtcaa tgggaatttt tcgcccgcaa aataagaaaa aactactggt aatttttgtt
     2881 ctttaaagcg attatacgtc catgccgcta ctaaaccgga aataattcca ccaaaaatcc
     2941 ccatattcac ttctgggtcc ataatttcta agcctttttt caaggtagca atcgctaaaa
     3001 aaccagtaag tgcaggtatt cctttgtcct tatttttgac aaaaccgatt gcaactccta
     3061 tagcaaataa gatatccaag tttccaaaaa cggcatttcc agctgcacca attactggga
     3121 tgttcagcat atcgtcagct gataaacgca tgatcaatcc cgctaatggc atcgctgcaa
     3181 ctggaagtag catcgctctt ccaagcttct gcacatttga tttaacattc atttttattc
     3241 ctcctctatt aaatatcctc atatgttact aactcaatca aaggatcatt tttcactttt
     3301 tgagcaaatg ctgaatgaat taaaaattcg acttggtggg tacgaggata cacaaaactt
     3361 gaaccattta acaattcctg atccaaatac gctgcgtgac acatgacttc gatcgtttta
     3421 tcttctctca atatcatgag taaacttgcc aaatattctt ccacactcgt attgatttgt
     3481 cgtttttttt catcagtttc tcctacgaca tcgaaacaag gctcaaaata atcgacatga
     3541 cgaacttcat cttttcgttc aaagttgctc cgaaccggca aatcatattt ttttgctaat
     3601 gtaacaacta cctcctgatt ttcatccaaa gtatgagtat gatgatgact gtctaaatga
     3661 gttggtatga tccccgcatg ataaattttt tgaatctgag cattccattc ctgatacagt
     3721 tgatctttgt caatgttaaa tggatgctga taataagata aggatctgaa ctctccgctt
     3781 tcgataagag tatcgacagt tttgaggagt ggtttgccac acgtcaatgt taaatggact
     3841 cctacaccta gcctaggcat ttcctttctt agtttcacag catgctcgaa acccggcata
     3901 tttgccatca atgtagttga tgtcaaaatt cctcgctgat aagcatccat aatcccataa
     3961 ttcacaccat ggctatagcc aaaatcatcc gaattaaaaa tcacttttcc catttcattc
     4021 tccttctttt ttctactaaa ataggcgcaa ccaaataatc atagtccatt tactatgtgt
     4081 catttggttg cgcctataga tagttacgcc cttattttaa tagattcttg ctagatgaat
     4141 tgccaaatag atgatttcat tttcttgaat ggcaagttga aattgctgtt cgatgatatc
     4201 tttaatcttt agtgctgtac aatacgcttt gctatgttta aatgtttcag taaaaaaacc
     4261 aatattttct actggcgttt cttcctgata aatcaagcgt ttaaccagga atttgatatg
     4321 aaccattaag cgatgataag tcagtgattc tttatcataa acgtttgaat aggatttttc
     4381 tagatgggac aaaatcgtgg tcgtcatttc tactattttt aagctcttta aactattagg
     4441 ctctcctagc tcagcattaa tgatatgcat agtgatgaat cctacttcat ttgaccctaa
     4501 ctcggtttga tatcgatgat tgattcgctc gaccacttct actgctatat catattcatt
     4561 tggataaagg aatctcattt ctggcaacat aattgctgtg acatctctat cttctctttt
     4621 tcgttgaata gcaaaataaa tatgatctgt caatgtaata tagatatttg aatctaatgg
     4681 agaggagatc ctagctgtta tcagttcaat cacttcttcc gcaaaatcca taaattcgat
     4741 tggaatctgc gagactatct cactgaatcg tttgatcttt tctttagaat ctaatacaaa
     4801 taatttgtca gcatcctcaa catcaaatgg cttactcact ttagcagaaa agccaatccc
     4861 tttccccatc gcaatttttt ctattccatc gtcatcgatc aatacagcat tatgattcag
     4921 ctttttcatc acatacaccg ttaattcacc tcctctatat gataaaacaa aacaacttgc
     4981 agactccttc atacatattt ttgtctccaa acaattttta aagacaaaaa acgacctaag
     5041 aaagaaccct ttcttaggtc gtgcctaatt gaatagtagc accctacata atttacaaaa
     5101 atagtttaac acataataaa agcgttatca atatcttttt tactcgtttt atgataatac
     5161 ttgtatcgaa ttgatttgga ggtaattaca taaaaacaaa aaaaatatag caactctcta
     5221 taagaaagtt gctatattga taattatacc ggcggccggg gtcgaaccgg cacgtcctca
     5281 acggacactg gattttgagt ccagcgcgtc tgccaattcc gccacgccgg caaatttaaa
     5341 aataataact ggggtagctg gattcgaacc aacgcatgag ggagtcaaag tcccttgcct
     5401 taccgcttgg ctatacccca agaataaagg gcgaatgatg ggaatcgaac ccacgaatgc
     5461 cggagccaca atccggtgcg ttaaccactt cgccacattc gccatcataa aagatgttat
     5521 ttaatttctc aataaaaaac taagatgaag acatcttagt caataccggc ggccggggtc
     5581 gaaccggcac gccctcaacg ggcactggat tttgagtcca gcgcgtctgc caattccgcc
     5641 acgccggcaa gttgatttat tagtactaag gcggtaaccg gatttgaacc ggtgatgaag
     5701 gttttgcaga cctctgcctt accacttggc tataccgcca ttatactatt atcaatctat
     5761 aaaataaaga ataaattata g
//
LOCUS       DAYYCW010000135         5526 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000135 DAYYCW010000000
VERSION     DAYYCW010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.137"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      61..227
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            375..932
                     /locus_tag="ACHAYA_002668"
                     /pseudo
     CDS             375..932
                     /locus_tag="ACHAYA_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            990..3029
                     /locus_tag="ACHAYA_002669"
     CDS             990..3029
                     /locus_tag="ACHAYA_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8606405.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGEKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKDDDDSANKRASELLQYLNQNYQ"
     gene            complement(3138..4289)
                     /locus_tag="ACHAYA_002670"
     CDS             complement(3138..4289)
                     /locus_tag="ACHAYA_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8606406.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4443..4973)
                     /locus_tag="ACHAYA_002671"
     CDS             complement(4443..4973)
                     /locus_tag="ACHAYA_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8606407.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5072..5422)
                     /locus_tag="ACHAYA_002672"
     CDS             complement(5072..5422)
                     /locus_tag="ACHAYA_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM8606408.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         2006 a         1017 c         1040 g         1463 t
ORIGIN      
        1 tcaaattcca ttgcctaatc attaggaaaa gtctatcatg aactcgtaaa tagaatatag
       61 taataacctt gttaggtgag gctcctatac aaacataggc tgttgcccag aaacgtcgaa
      121 agtcgccaat gggtaaaaca gaaactgttg gattaaggct ttttccaata gctaagaatg
      181 ttttctttac gttgtatagt gctaaaactc ggacgatgag atgatgctag gtcagaattc
      241 ttgttcgaaa aggtttctga ccttttcttt atatttttgt gactgactta tttgatgaga
      301 caaactcgct cctcacctga caagtggatt gctgtgtgag gaggagatgc ggtgaaaaaa
      361 gatcgattgg tggaggtcgc tcggattcac ttctggttgc acaatacaac cagaaaacac
      421 aacaagcaaa actgatctct atcatgagag attcatatgt cgatattcca ggttacggaa
      481 tgaataagat aaatgcagct tactcttatg ggggagttga tttattaaat caaacattaa
      541 aggaaaattt caaatttgaa gctccctatt atgcaagtat cacatttcaa gattttatcg
      601 attgcgttaa tgaactgttt cctggtggag tcaagatcga tgcagaaaaa tctctagatt
      661 tagatggcgt atatataaac aaaggagagc aagtgatgga tggtaatact ttactccagt
      721 atgctcgatt tcgggaagac aaagaaggag acttcgggag gatcagaagg cagcagcaag
      781 tgatcaaagc tatctctcag cagctgaaag acgtaacttc aatattcaag ctacccaaag
      841 cggttgggaa attacttggt agtatccaaa cgaaccttcc agaaagtgtg cttctggatt
      901 gtggtatgga tttcctcaaa gacatatgtt agaataaaca ggtataaata gtgaaataaa
      961 ggaatgacaa gcaagagaag gaggaaaaaa tgaaaagaag tgacaagcac ggcaaaaatc
     1021 gaacaggcgc ttatattgcc ggcgcagtga tcttaatagc agctgcgggt ggcggttatt
     1081 tttactacca gcactatcaa gaaacccaag cagtagaagc tggagaaaag acagttgagc
     1141 aatttgtcca agctttgaac aagggagatt ataacaaagc tgcagaaatg acatcaaaaa
     1201 aggcggcaaa taaaagtgca ttatctgaaa aagagatctt agatcaatac cagaatatat
     1261 acggtgctgc tgatgtcaaa ggacttcaga tatcgaatct aaaagtagat aaaaaagacg
     1321 attctactta cagtttttca tataaagcaa agatgaatac ctcattaggt gaattgaaag
     1381 atctttctta taaaggaaca ttagacagaa atgatggtca aaccacgatc aactggcaac
     1441 ccaacttggt ttttccagaa atggaaggaa atgacaaagt aagcctgacc acacaagaag
     1501 cagcaagagg gaatattata gatcgaaatg gtgaaccatt agcaacaaca ggcaaactaa
     1561 aacaattagg agtcgttcca agtaaacttg gagatggagg cgaaaaaaca gccaatatca
     1621 aagcgattgc ttcctccttc gacttaacag aagatgctat caatcaggcg atttcacaaa
     1681 gctgggtaca acccgattac tttgtcccat tgaaaatcat tgatggagca acgccagaac
     1741 ttccagctgg agctaccatt caagaagtag acggcagata ttatcctttg ggtgaagcag
     1801 ctgctcagct gattggttac gtgggagata tcacagcaga agatattgat aagaatccag
     1861 aattaagcag taatggaaaa atcggtcgct caggtttgga gatggctttt gataaggatc
     1921 ttcgtgggac aacaggtgag aaattaagca ttacagatgc agacggtgta gagaaaaaag
     1981 ttctgatcga acatgaagtt caaaatggca aagatatcaa attgacaatc gatgcgaagg
     2041 cacaaaaaac agcttttgac agtctaggag gaaaagctgg atcaactgtt gcgacaacgc
     2101 caaaaaccgg tgatcttctt gcgcttgcta gctctccaag ctatgatcca aacaaaatga
     2161 caaacgggat ctcacaagaa gattacaaag cttatgaaga aaatcctgaa catccattca
     2221 tcagccgatt tgcgacaggt tatgctcctg gatctacgtt taaaataatt acagcagcaa
     2281 tcggtctcga caacggcact atcgatccaa atgaagtgtt gacgatcaac gggcttaaat
     2341 ggcaaaaaga cagttcttgg ggatctttta aaacaacgcg tgtcagtagt gatgtatcac
     2401 aagtagactt aaaaactgct ttgatctatt ccgataatat atatgcggca caagaaacgt
     2461 tgaaaatggg tgagaaaaaa tttcgtacag gcttagataa attcattttt ggtgaagacc
     2521 ttgatttgcc aattagtatg aatccagcac aaatttctaa tgaagatagc tttaactcag
     2581 atatcttgct agctgatact ggatatggac agggcgaact tctaattact cctatccagc
     2641 aagcagcaat gtattctgtt tttaccaaca atggcacact tgtctatcct aaattgattg
     2701 cagataaaga gacaaaagat aagaagaatg tcatcagcga aacagcagta caaacgatcg
     2761 tgccagatct gagagaagtt gtgcaagatg taaatggtac agcacattct ctttctgctt
     2821 tagggattcc gttggcagcg aaaactggta cagcggaaat caaagaaaaa caggatgtaa
     2881 aggggcaaga gaacagtttc ttgtttgctt tcaatcttga taaccaaggt tatatgatgg
     2941 tcagcatgtt ggaaaataaa gatgatgatg attcagcaaa caaacgagca tcggaactat
     3001 tgcaatacct caaccaaaat tatcaataaa aaattgcttt ttaagtacag taaaaggact
     3061 gtggcgttat cgtcacagtc cttttttcaa ataaacggtt ttcacgatgt ctctttcaca
     3121 acttcttttt gatttactca cacttcataa actttttttg taaaatattt gagaacaagt
     3181 ggagaaataa gtgtacttaa taaaatcacg ataactaaag gtgaatagta ctgtggttcg
     3241 atcaattgac tttgctgtcc aatttgtaaa atgatcaaag ccatttctcc acgcgaaatc
     3301 ataccagcac cgaccatcaa tgaactatgt tgagaaaaac cggcaatctg agcaccaaaa
     3361 taacctccaa gcaatttggt caaaatcgct acaagcgtca atatagaaat aaagagtagt
     3421 tgttcactaa agtcagaaaa atcaacttct aatcctacac tgacaaaaaa tactggtata
     3481 aatactgcat atcctaaagc ctctacattg tggtatactt cttctttaac ctttgtctga
     3541 ctgacagcta tcccagcaaa aaaagcacca atgacagagc taagcccaac aagatccgct
     3601 acgtaagaca ttcctaaaca aataactagc gatacgatga taatagaaga atttgcgtac
     3661 actttttctg caaatgaaag taggaaaggc gcaatccatt tgactaataa gaaaataaaa
     3721 acaaaataaa ttagctgttc aatcaaaaca agcggtaaag aagcattgct agaagcttcc
     3781 cccgttagaa aagataggct gaaacttaga actaaaacga ctaaaatgtc atctaccaca
     3841 gaagcaccca aaattgttga tccttctttt gtattcacta cgtttagttc ttttaatact
     3901 tctactgaaa tactgacaga agttgccgca agaataatcc caaagaaaat cgcttcattt
     3961 cccgaaactt ggaatgcttt gcctgtcaac cagccaaaaa caaccgggaa gatgatccca
     4021 agcaaagcaa cgaacatacc aggtcggaaa tattttttca aaagagataa atcactttcc
     4081 agaccagcta aaaacattaa taagaacaca ccaatctccg aaaagtcatg gactagaata
     4141 tcaggtttga cccaattcaa tccagcttgg cctagcaaaa caccaacaag taattgtcca
     4201 atcactgctg ggatacctaa tctccgcgag atatgtgagc caatcgttgt tgaaatcaat
     4261 atcaggcata aaataccaat aaattccatt ctcatcattc ctttgtataa aatatagccc
     4321 gaacttagca taacatacat tattgaaaaa ataggaaaat atactcacaa aaaaaccaac
     4381 aatccaaaag atttttttgg attgttggtt tttatacggt ctattttaat aactaaaaat
     4441 gattaacgta tagtcatttt tcttattctg atgaaattga taatctcctg gatgcccatg
     4501 aaaatcaaaa taaagccgaa tatttggaat agaagcatca aacttttgaa tggattgaac
     4561 aataagacga atccgccaat caacaacaga atactactat aaacactcca acttttagct
     4621 tttgtattca tgctgaagaa caactggaac aagctgttga tgataacagc taatccaaga
     4681 agaatcggca ggatagaaac gattgcaggt gcaaaagcat agacgatcag agctaagatc
     4741 aggaaaaaaa gaccactgaa cagaccgata ctccaagaac ctgttttcct tttaaaacgg
     4801 taagcctcaa aaagattcaa caacccaagc aaaacaagat aggcggtgat cagataaccg
     4861 ataaaatgaa aaacagcaga agggtttagc acaatcgcaa tcccagaaat aatataaaca
     4921 gcagctctta ataaggcgta tttttgaata ttttgtttaa tcgtctcaaa catataagaa
     4981 aacctccttt gacttatttt aacgtgaact agtcatttta ccaaattaaa tgttacaaaa
     5041 tgagcgaagg agaagaatat ctctggaaaa atcaacttct gaggatcttt tccatttttt
     5101 tgccttttgc tagctcatcg atcattttat ctaaataacg aatttctttc atcaagttat
     5161 cctcgatgtc ttctactcgt acaccacaaa caacacctgt aatcaaggaa cgtgaaggat
     5221 tggggacagg ggcttccgca aaaaattgtt cgaaagtcac ttgtttttgt agttgatatt
     5281 ctagttctgt ttgggaataa cccgtcagcc aacaaatgat ttcatctact tcctctttta
     5341 tacgatcttt tctctccgct tttgctacat acataggata aacgcttgca aaacttgtcg
     5401 taaaaatacg attttgcgcc ataatcagtc acctttcttt ttttcaaatt atttactact
     5461 tatagtatat aagaaaaaga gaagaaaaag cgattattca aaaaaacaaa aaggattgtg
     5521 acaaaa
//
LOCUS       DAYYCW010000136         5496 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000136 DAYYCW010000000
VERSION     DAYYCW010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.138"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..700
                     /locus_tag="ACHAYA_002673"
     CDS             <1..700
                     /locus_tag="ACHAYA_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606409.1"
                     /translation="RLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSALANQVQDRL
                     SFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNKQELINWRLT
                     GFSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVLKTTELPDIR
                     DILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLILSLFPFTFF
                     IHHLQSRRPGRNET"
     gene            690..1199
                     /locus_tag="ACHAYA_002674"
     CDS             690..1199
                     /locus_tag="ACHAYA_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606410.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1269..2717)
                     /locus_tag="ACHAYA_002675"
     CDS             complement(1269..2717)
                     /locus_tag="ACHAYA_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8606411.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2717..3433)
                     /locus_tag="ACHAYA_002676"
     CDS             complement(2717..3433)
                     /locus_tag="ACHAYA_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM8606412.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3414..3764)
                     /locus_tag="ACHAYA_002677"
     CDS             complement(3414..3764)
                     /locus_tag="ACHAYA_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8606413.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            3908..4681
                     /locus_tag="ACHAYA_002678"
     CDS             3908..4681
                     /locus_tag="ACHAYA_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8606414.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            4852..5218
                     /gene="ssrA"
                     /locus_tag="ACHAYA_002679"
     tmRNA           4852..5218
                     /gene="ssrA"
                     /locus_tag="ACHAYA_002679"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5264..>5496)
                     /locus_tag="ACHAYA_002680"
     CDS             complement(5264..>5496)
                     /locus_tag="ACHAYA_002680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606415.1"
                     /translation="FEGTCLNHNCVFPSISIKVHLKKSVRTKVLFVNLKIDFPEYVLG
                     ISKCLNVFLNAKSMQKILYFSTFLSLTKIKKR"
BASE COUNT         1594 a         1084 c          962 g         1856 t
ORIGIN      
        1 tcgattaatt tattacaaaa agcagacaat actctattgt ttattttcaa gcttttctgc
       61 ttttttattg atagcatgcc tgactcttta ccattcgcaa tcggctttag ccaatcaagt
      121 ccaagatcgg ctttcattta ttggcgcttc tgcatctaca gagcttcttc cggctttaga
      181 attagctagc cgattttata taaaaggcat attcagttta ttttttctat ttgccttcct
      241 ttttatcttt ttcttttact attcagtcag gcaaaacaag caggaattga tcaattggcg
      301 cctgactggt ttttctaaaa ccaaattatt cctttttatt ttctggcagc tgttctttcc
      361 tctctttctt tgttgtatct tggttttatt gtggatcgtc agcttccagt cagtttatga
      421 agcgcttctt caaaggatca attttcttgt cttaaagact actgaactgc cagatatccg
      481 tgatatcttg acttcggtaa gcggacttac aattccgatg gatcaacaga ctttttttac
      541 catcgatttc caaaatgatc tttttttcac agatatactt aagggatttg cccaaactct
      601 tattgggctc agtggatgct gtctcattct tagtctattc cctttcactt tttttatcca
      661 tcatttgcaa agtaggaggc ctggacgtaa tgaaacctga atatttgatt catgctgaac
      721 acttgaccgt tattttgtgt cctctcgaaa aacagcataa aatattggag cacttgaaaa
      781 aagaggttct tgttgcaaaa ctggataatt ttgcgatcat ccaaaaaaat tggccgatgt
      841 ttccttatct gaatcttaaa gaccatgtcc tgttggatgt accggaaaaa gaaatcaaac
      901 aagatcgttt ggcctatcaa gaaaaacttg atatctctcc ttccttgctt aattgtgcgg
      961 cagctgagtt gacgtctttt gaaaaagtaa agctgcaatt acttcatgcc ttgctttcca
     1021 agcgaaacaa tattgttatt gaagatacat ttgatgaact tagtgtattg gagatccaag
     1081 agctactcca cttgctttct tatttagctc atgaagaaaa tcaaggtatc ttactcttta
     1141 ctcatgatgc tactattgct caaagtcctt atattgatcg tttagagccc gcgggctaaa
     1201 tcaatcattt tatagtaaaa acaagcggta tagaagatcg attcatgatc ttttataccg
     1261 cttgtttttt actctttttt gatttctacg tcaggcaaac gaacagcgaa ttttgttcct
     1321 tttcctagtt cgctttctac ttctaatttt cctttatgca agcgtacaag ttgctgcacg
     1381 attggtaaac ctaatcctga ttcaccatat tttgtatttt ttcttgaagg atctacttta
     1441 taataacgat cccagatact tttcatttgt tgttcagaca ttccaattcc cgtatcttca
     1501 atcgtgatga tcgtttcaag atagcctttt tctaaagtaa tcgtgatttg gccgttttca
     1561 gtaaactgta tcgcgttttg aataatattg accatgattt ggacaaatcg gtcataatca
     1621 gcgtaaacat caatatcatc aacggctttc aatgtcaatg tatctcctgc tgcttctgct
     1681 ttagcttcta actgtgcaac aatatttttc aatgtttctg ttccattgaa ttttttaatg
     1741 accatactga tttgattcgt acggattttt tcataatcca agttttcatt caccaaacgg
     1801 atcagacgct ctgtttcatt tttcatcaat ttaatcgcgt tttctcgctg attttcaggg
     1861 attgcattgt attctaaccc ttccagaagt ccgttgattg tcgtaagagg tgttctcatc
     1921 tcgtgagaag catctgccat aaattgtctt cgacgatttt cttgttcttc aatttctgct
     1981 tgagattctt tcaatgaatt cgtcatttta ttgaaatcat ctgctaactc atcaaattca
     2041 tctttgtcat gtacggggag ctgtacatca aaattaccat tcgtcacttc tttcgtcgca
     2101 ttccttaacc gattgatacg cttaacttgg aatgctgcaa aagcatagct ggcaataact
     2161 gcaacgatac tggaaaaaat aaatcctttg aacaggttca gtgtaattgg acgaacgctg
     2221 ttatcgatat ttcgcgcagg ttgactaact acaagtgctc catagaattc atgattcaaa
     2281 ttaaatggca caagtgcgta agaggtcgcc tgtgcttgtc ctaaaatatt tttattcgat
     2341 gtgaattttt cttgacgtcc attcctcaga tttttccatt gatcagctgt taaggtaaaa
     2401 ttcagtaaag ctgtattagt cggataaatg acacgttcgt ttttatcgat aaatataaag
     2461 ttcacatttt gttctgtcag tacttgctct gtcagcagca atgcattttg aaacgcctga
     2521 tcttgactaa actgcgggag cgtatcggca taggtttgcg ttgtcttttc aactgattca
     2581 gcatatccaa acagttgctg gtaattgttt tcttcaatcg tttgttttgt cagttgtgta
     2641 aaagaaactc caacgatcaa taagatcaac gcgaccattc cgcaaaaagc taagagttgc
     2701 tgatacaaat aacgcattag gcaacaccag aatcatcgaa tttgtagccg actccccaaa
     2761 cagtttggat gacttgtggt ccgacctttt cgattttttg tcgtaatttc ttgatatggg
     2821 cgtccactgt ccgttcatca ccaaagtact gataatccca gactaattca agcaactgct
     2881 cccgagagaa tacttgacgt ggtttttttg ctaaagtaaa caatagatcg aactctttag
     2941 gcgtcagtcc ttcaattggc cggttgtcca gatacgcttc tcgtgttttc gtattcatct
     3001 tgaaatgact agtctcgata tcaaaatgat catctgaaac tgctgcttct tctcttgctt
     3061 ctgccaactc actacggcga tgcaaagctt tcattcgagc aataagcgtc agaggactga
     3121 aaggttttgt gacataatca tctgccccca tctctaagcc gatcacctga tcgctttcag
     3181 aatctctagc tgtcagcatg ataatcggca cagtacttga aactttgcgg atctctcttc
     3241 ctactagcat accgtccagt gtgggcaaat tcaggtctag tgtaatcata tcccaatgtt
     3301 gtgggttttc taaaaaggca tcgagccctt cttttccatc atacttgaaa gttgcttccc
     3361 aaccttcatt taagaaaaac atctgcatca tttcagaaac agattgatta tcttcaatca
     3421 ttaaaatatt cattatcgtt ccctccagtt tccaggtcat atttctgtaa tttcttttct
     3481 gtcataataa aaataggcat aattggaaat actagcagaa gcatttcaat cagaaaataa
     3541 ttcgtatgcc cagatgtctt taaataatag ccgatcaatg ccatcagtaa gcccataaga
     3601 aaaaaccagt ttccgcttgt cttttgagcc agccgccaat gagcgtctgt ttttgcggca
     3661 agaggcgaat gatacccata aaaaggcaga tttccttttg agggcagaat ccgaaaaaga
     3721 ctgccaatta ttaagaataa gataccaata ctgaaaaaaa tcattctttc cctcctaaat
     3781 tctcttctta tagtatactc taaattgttg actgaaccaa tatgttcagg aattcttcac
     3841 gaaaggtttg tcttttgttc attctttgtt aagataaact tattccaata aaaaaggagt
     3901 tttatttgtg tcaaattatc gtgcaatcac tttctttgat cttgatggaa cattactaga
     3961 tgcgaaatca aaaatcacac ccgaagtggc aaaagctatg aacgcattaa aagaaaacaa
     4021 tgttctcccc gtcatcgcta caggacgtac agaagcagaa atccactcta tcatggaaga
     4081 tgcgggcatt acttctgcgg tcaccatgaa tggttctttc attcgagtag acggcgaaga
     4141 aatctattct caaacatttt cttcagaaga atgtcaaaaa atgtatgatc atgtacagtt
     4201 gaacggtcat gaactttctt actataacga tcagaaaatc tggtgtaccg gtcatagcca
     4261 aacaatgatc aatgcttacg acttcattca ttcagatgtt ccggaaattg atcctttagg
     4321 ctttaaagaa aacacagtaa atatgttgct gatcttatct caatcaggtg acgaatacta
     4381 tcacgaacat tttcctgagc taacttttta tcggaatggt ccttactcga tcgatacagt
     4441 gaagaaagga atctcaaaag gggcaggtgt caaacgtctg caaaaagaac ttcagctgga
     4501 aaaagttcct acttttggat ttggcgatgg tccgaatgat tttgccttgt tggaagcttg
     4561 cgacaacaaa atcgctatgg acaacgccta tgacggatta aaagaacttt ctacctttat
     4621 cacaaagaaa aatacagaag gcggaattgt ccacgcattg aagtattttg atctgattta
     4681 agatcttttt ttgaagctac ctcttttctt aagaaaatcg cttatttttt cttaagaaag
     4741 gggcagttat tatataaata atgggatatg attcgctttt ttattgaatt tatcccaata
     4801 tatgctatac taattttgta tgaaaaatac acgtaaccct tgttagctaa cggggacgtt
     4861 acggattcga caggcacagt cgagcttgaa ttgcgtttcg taggttacgt ctacgtaaaa
     4921 acgttacagt taaatataac tgctaaaaac gaaaacaact cttacgcttt agctgcctaa
     4981 aaacagttag cgtagatcct ctcggcatcg cccatgtgct cgagtaaggg tcctaacttt
     5041 agtgggatac gtttcaactt tccgtctgta agttgaaaaa gagaacatca gactagcgat
     5101 acagaatgcc tgtcactcgg caagctgtaa agtgaatcct taaatgagtt gactatgaac
     5161 gtagattttt aagtggcgat gtgtttggac gcgggttcga ctcccgccgt ctccatatat
     5221 gtatggttga gacagataaa catgtatgaa aaacgttgtt aaatcaacgt ttttttattt
     5281 tggtcaagga gagaaaagta gagaaataca aaattttttg cattgatttt gcattcaaaa
     5341 aaacattcaa acatttagaa atccccaata cgtattcagg aaaatctatt ttaaggttta
     5401 caaaaagaac ttttgttcgt acactctttt tgaggtgaac ttttatgctg atggaaggaa
     5461 aaacacaatt atggtttaag catgttcctt caaata
//
LOCUS       DAYYCW010000137         5418 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000137 DAYYCW010000000
VERSION     DAYYCW010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.139"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..914
                     /locus_tag="ACHAYA_002681"
     CDS             123..914
                     /locus_tag="ACHAYA_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8606416.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            880..1833
                     /locus_tag="ACHAYA_002682"
     CDS             880..1833
                     /locus_tag="ACHAYA_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFM8606417.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2231..3205)
                     /locus_tag="ACHAYA_002683"
     CDS             complement(2231..3205)
                     /locus_tag="ACHAYA_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8606418.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3320..3754
                     /locus_tag="ACHAYA_002684"
     CDS             3320..3754
                     /locus_tag="ACHAYA_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8606419.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(3879..4901)
                     /locus_tag="ACHAYA_002685"
     CDS             complement(3879..4901)
                     /locus_tag="ACHAYA_002685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFM8606420.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5293..>5418
                     /locus_tag="ACHAYA_002686"
     CDS             5293..>5418
                     /locus_tag="ACHAYA_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HFM8606421.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHG"
BASE COUNT         1720 a         1026 c          960 g         1712 t
ORIGIN      
        1 cgctttcaaa agaaatttat tttgttaaat ttgatctact tgaaaataaa atcgtttatc
       61 tatataatta agaagaacaa agtcgaacaa aaattgactt ttatgttaaa ggagtaattc
      121 atatgctgaa aaaagaaaga caagaacaaa tcattacatt attagagaaa gatttttatt
      181 taactaattc agaaatcgct aacaaattag gcgtatcaga aatgaccata cgccgagatg
      241 tcactgaatt agcaaaaaaa aataaagtaa aaaagctata tggtggcgtt gaacgaaagg
      301 aaccttataa tcgagaacta tcgactcaag aaaagattga tacgaatgta gccgaaaaaa
      361 aatatattgg tcaagtcatg aacagtatga ttcccgataa ttctactatt tatgtcggtg
      421 ctggaacaac gatcctatat gcacttcctt tcattcaaaa gaaaaatcta tttgtaatta
      481 caaacagttt gattgctttc aactatttaa agaatcatac aagttatcgg attttactta
      541 ctggtggcga attttctcct attacagaag agttcattgg ggaagttgca gaaagatctt
      601 ttgaaagttt gaatgtcgat atctctttcg ctgcaacaaa cggcatattt gataacaatg
      661 tgacaaccgc tcaatttatt gagggaaata ttcagaacgc agcgctcaat gcagcaaaaa
      721 tcaaatgcat tgttgcagat tattcaaaat taaataaatc agacgtctat acttttagaa
      781 aattaaccga ttttgattat ctaataaccg ataatcattt atcagaacag gatttcaatc
      841 attattcaaa atacttaaca atattaaagg agcccaaaaa tgatactgac aatcacgatg
      901 aacccatcca ttgattccat ttattttaca aaacactttg aattaggtac gatgacacgc
      961 tttgattctc cggtacgcta tgtaggcggc aagggaatca attgcggacg agcttctgct
     1021 ctactaggtt catcagttat tttaacaggg tttttgggcg gttattttgg cagtatgatt
     1081 caagatatgc tgaaaaatga aaatttgttt gatttgcatt ttttacctat tgcagaagaa
     1141 tcaagaggtg ccgtcacgat catgcatgat gatgaaacgc agacagagct agtggaagct
     1201 ggtcctgaaa tagcagatac tactatcaaa aaattaatga atgatatcaa actaatcagt
     1261 aaaaataatt acctttcagt cattacaata aatggttctg ttaacaatcc agatcccttt
     1321 ttgtatgcta aattattaca atatatcaaa gaacaaatag atccttccac ctatatatta
     1381 atggatatat ctggacaaca tctccgtgaa gtcttagcaa gtaagtataa accgatgttc
     1441 atcaaaccta atatccatga gttttcagat atcatccatc aaaaaattac tcaaaaaagc
     1501 gacataaaac accatctaaa aacaaatcca ctatttaaag atatcgatgt tatcatgatt
     1561 tcttgcggct cgtatggtgc agtagtcaaa gtaaaagaag aattttggaa tgttgagatc
     1621 cctaagattc ctatcattaa tacaacaggc tctggggatg ccactgttgg aggttttgct
     1681 cattcattag aacagaatta tcctttaaaa gaatgtgtaa ggtacgcaat ggcttgcggc
     1741 atgtcgaatg cacagcaatc agaagtcggg aaagttattc cagctgatgt aaaaaattta
     1801 gcaaaaagca ttaaaatcac agaaatgaaa taaatagtac aaagtaaata attgtttata
     1861 tgaaatgaga cacaaagaga ctgtgacaaa aaacgtgtgt accgaccccc aaaagttaga
     1921 ttttttggtc taacttttgg gagtcgatac attgtcacag tccttttttc gaataaacgg
     1981 tgttctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctatt
     2041 ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacgacct cttggtataa
     2101 acggtgttca aaagcttgca tctacgctaa tgagtccaac caagtcaaaa atgaggagct
     2161 tttttggctg tccttattac ttgatgcagg gcagtttttt cataatctct tttagattaa
     2221 atccttttgg ttacacaagg ttctcttcaa agaatgttgc cattttttca aatggaatga
     2281 tgtctgtctg gtcgtataga tctgtatggg atgctcctgg aatgatcatg agttccttgt
     2341 tgtcaccttt tagttctttg aaagcgtcct ccccaaagta acgtgaatga gctttttccc
     2401 cgtgaatgac taagactgca tgacggattt ctggaatata atgcaatagt tttgcattca
     2461 taagtgacaa ccctgatgtc ctattccaac catcattgga gtttaaagaa cggacatgat
     2521 atccgcggtc tgttttataa tacgcatgat agtctttgac aaattgagga gcatcttcag
     2581 gcaacggatc aaccacacct ccagcacgtg cgtagtcccc ttgtttgaaa tcaatgatgc
     2641 gttgtgcatt taattcttca cgcatttcat atcgggcatt ttcatccata acatcgaagt
     2701 agccatttgc tgttactcga gtcatgtcat acattgtgga aacaactgtt gctttgaccc
     2761 gcgtatcgat agctgctgca ttcaatgcca tacctcccca tccacaaata ccaatgattc
     2821 cgattctctc agggtttaca tttttttgta cagataaaaa gtctactgcc gcttggaaat
     2881 cttccgtatt gatatcaggc gaagcaacat atcgaggtac tcctccgctt tctcctgtaa
     2941 acgatggatc aaacgcaatc gttaaaaagc ctctttcagc catcgtctgc gcatatagac
     3001 cagacgattg ttccttcact gcaccaaatg gcccgctcac agcgattgcc ggcaatttct
     3061 tttcacttac ttcttttggt tcatataaat cagcgactaa tgtgatacca aaacgattcg
     3121 ggaagctcac ctttcgatga ttgacttgat tgctttttgg aaatactttg tcccattctt
     3181 gtgtgatgtt taattcttta ttcatattct agactctcct ttgatcaaaa ttgacatttg
     3241 tttttgaatg tattattatg ataaaactta aacttgactt taggtcaagg taaaaatttt
     3301 attttaggag gattttttta tgtattccat tggacaagtg tctgaaatgt ttggattgcc
     3361 tgtttctact ttacgttatt acgacaaaga gggattattt cctgatatcc agcgtgtttc
     3421 tggtattaga aaattttcag agaatgagtt agaggcttta agagtcattg actgtttgaa
     3481 aaaatctggt ttagaaataa aagacattaa gcaattcatg gaatggagca aagaaggcag
     3541 taagacattt gaggttcgta aagaactatt tgaaaaacaa aaagaagtag tagaaaaaga
     3601 aatagcaaaa ttagaaagag tactcgctat gctaaactac aaaagctggt actatgaaga
     3661 agcaataaaa gccggcaatg aagaagccgt attgacaatg attcctgatg atttacccca
     3721 acatgtaaaa gaagcttatg tacattctca ttgaacaaac ggataactca ctctccttct
     3781 ctatgaactt catgaaaaca tgaataaaca cctctaactg attgttagaa gtgtttattt
     3841 tttcactcaa gtatcctgca cttacaaaaa tatccttttt accgccatcc taattctgga
     3901 gcgatatctt ttacgattgt ttcaagcaag tgcatgttat attctacccc caaagtatta
     3961 ggaatcgtga caagtactgt gtctgcttct ttgattgctt catcttcagc tagctccttg
     4021 attaattgat caggttcacc agcaaaagtt tttccaaaaa tcgttgggtt tcggtgataa
     4081 gcaagatagc cgacagcatc ttgttgcgga ctaccgtctt gtccaaataa gcgtcgatcc
     4141 aaatcagtgg tgattggctg aatcgagcga gtaactaatg ttcttggttc aaaatcatga
     4201 cctgcttgct tccacgcttc tttatacgca cgtaattggt ttgcttgttg gatatggaat
     4261 ggctcgtggg attcgtaatt gactaatgta gatgattgca gattcattcc atgttgtgct
     4321 gcccagattg ccgtttgctg cgaaccagct ccccaccaga ttctttgtcg taatccttct
     4381 gaataaggtt ctatccgtaa ttttcctgat ccttgtggga acattggttg aggatttggt
     4441 tcttcaaatg gttttccttc aagtaattcc aagaattcaa gggtgcgttc ccgtgttacc
     4501 tctttgattt cttcttcact gtaatcataa ccaaagtatt tccagccatc tagaacttgt
     4561 tcaggtgaac cacgccccac tcctagttgc actcttcctt gcgtgatgat atctgtcaat
     4621 ccggcatttt ctgccatata ataagggttt tcatatcgca tatcaataag tcctgtaccg
     4681 atttcaatct tacttgtctt tgcgccgatt gccgctagca aaggaaacgg cgacccaatc
     4741 tgaggggcaa aatgatgtac gcggaaaaac gctccgtcca ctccgatttt ttctgcttcc
     4801 accgctaaat caatcgattg caaataagca tcttgagctg atttcacaag cgatccttgc
     4861 tccatccaat gtccaaagct taaaaagccg atttttttca aggtaatgac ctcctttaaa
     4921 cctacaaaaa tggaaggttt tttattttta atatgaactg attctactac tttttttctt
     4981 aaaagacgaa taattgttct cgcctcacaa tcaagaaatg tatcggctta ctacacccct
     5041 actttcttca tcatctgccg tgtcttaatc tcttttaaca gtctgttaca tcgttgattt
     5101 ttgactcaaa atgattgttt ctaacatgta tttatggtaa aaaagacaag attagttctt
     5161 tgtacacatc gatttatacg ctgaacaaaa agaaatatat aaactaagaa acccattgtt
     5221 ttttcgctcc atcacataga gtttacttta gataaacagt gacatttact aatccataac
     5281 ggaggaaatc aaatgaaaaa aatctatttc ttatgtacgg gcaattcatg ccgcagtcaa
     5341 atagcagaag gctacgcgcg taaatacttg tctccttcaa tgtttgagat aagaagtgcg
     5401 gggatcgaga cacatggc
//
LOCUS       DAYYCW010000138         5413 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000138 DAYYCW010000000
VERSION     DAYYCW010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.140"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..993
                     /locus_tag="ACHAYA_002687"
     CDS             <1..993
                     /locus_tag="ACHAYA_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606422.1"
                     /translation="YVNWEGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIR
                     GIEIAIGRKAFPLGLKEEDKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFN
                     SRNYTNENMELLRKDFEVVYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQF
                     VRTLWADGQVFLPERIYLLSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKS
                     LSLINSEQAGRQEIEIFVVSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDE
                     CLCAKKRVVLCDAASYQNGLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1214..2977
                     /locus_tag="ACHAYA_002688"
     CDS             1214..2977
                     /locus_tag="ACHAYA_002688"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM8606423.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3061..3519
                     /locus_tag="ACHAYA_002689"
     CDS             3061..3519
                     /locus_tag="ACHAYA_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM8606424.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3628..4311
                     /locus_tag="ACHAYA_002690"
     CDS             3628..4311
                     /locus_tag="ACHAYA_002690"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8606425.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4458..5072
                     /locus_tag="ACHAYA_002691"
     CDS             4458..5072
                     /locus_tag="ACHAYA_002691"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM8606426.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5088..5294
                     /locus_tag="ACHAYA_002692"
     CDS             5088..5294
                     /locus_tag="ACHAYA_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606427.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         1937 a          849 c         1107 g         1520 t
ORIGIN      
        1 tatgtcaatt gggaaggcta tgaaaataaa aatccgtatg tagaagaagc ttgtaacgag
       61 ttgattgatt atgtagagac tcattttttt aaaagaagat attcggaaga tgaaaagtcc
      121 tttatcatac gaggaataga gatcgcaatc ggaagaaagg catttcccct tggactgaaa
      181 gaagaagata agaaaagcgc agaaacgaag ccgttatttc aattagtctg tcaaggattg
      241 gaagagcaaa aaggaattct agacttacaa gaagaagatg tctattatat tttttctttg
      301 ttcaacagta gaaattatac caatgagaac atggagctgt taagaaaaga tttcgaagtt
      361 gtttataaaa attttgttct caatgaccta tcactgaatg aattgatttt acagattatt
      421 agtatcgctg aaatcgacga tgcagaaaat tttattttca agcaatcctt tttacaattt
      481 gtccgtacgt tgtgggcaga tggacaagta tttttaccgg aaaggatcta tcttctatca
      541 gaaaaagaac aacttctgta cggaaaaatt gtagaaatat tggaagagtg gaaaaagaaa
      601 aatcaaatcc aactaagatg gaatgaaaat ttcattcgaa aattcgtcaa aagtctttct
      661 ttgatcaaca gtgaacaagc ggggagacag gaaatagaaa tttttgttgt ttcagaaagt
      721 tcagtcaaac aattttttta tcgaacccaa tttcatttgt atgcggaaga aggcatcgct
      781 gaaataaatc ctattttatt tcgttcatta gaagaattgc ctgatgaatg tctatgtgcg
      841 aaaaaaagag tggttttgtg cgatgctgct agctatcaaa atggattaga aactgaaaag
      901 acaaagatct atccaatctc attaaaaggt gcaaacatcc atttgtatca tattgctcag
      961 gaacttcacc tattaaaaaa acatatttct tgatgagacc ttaattagga gaccttctac
     1021 aaagcttttt aattgtctgt agaaggtttt tatttgagtc ctgattcgtg cgaagatcta
     1081 cttatataac aagctttctc tgaaattttt ttaaaatgct attgacttag agtcaactct
     1141 aagtagtaga atagataaag tgtaacaaat ctattttcat taaaagaaga tagtacggag
     1201 gagagaatgt attatgagta cagtattaca tgtattggat gcaatcaata gccccgcaga
     1261 tgtcaaaaaa ttatctgttg aggaaatgaa acagttggca gaagagatgc gccaaaaaat
     1321 cttgcaaaag gacagcgatg taggtgggca cgttggtccg aatctaggtg tagtagaatt
     1381 aacgattgcg tttcattatg tatttgattc accaaaggat aaaatcgttt gggatgtttc
     1441 ccatcagtct tatggtcata agcttttaac tggtcgaaaa aatggttttg aaaaaggaca
     1501 ttaccgtgaa gtgagcggat attccaacca acatgaaagt aaacatgact attttacagt
     1561 ggggcacaca tctacatcta tcagcttagc aacaggttta gctaaagcga gagatctgaa
     1621 gaaagaatca ggaaatgtgg tagcatttat cggtgacggc tccctaagtg gtggcttagc
     1681 ttttgaagga ttgaacaatg gtggtgtttt acaatccaat ctaatcgtat tagtaaacga
     1741 taatgagatg tctattgatg agaatcatgg cagtttatat aaaaacttag ctgagcttcg
     1801 tcaaaccaac ggtaaaagca gcaataacct atttaaagca atgggatttg attatcgtta
     1861 cgtggataat ggaaacgatc tggaaacggt gatccgttta tttgaagaag taaaagatat
     1921 tgatcatccg attgtacttc acgtccatac ggaaaaaggt cgcggttatc agccggcaat
     1981 cgataataaa atgaaatatc attggcatgt accatttgat ctgtccaccg gggaggcaaa
     2041 gcaagtaact tctggagaaa catttaatat gatcatcatg gattatttgg ataagaaaat
     2101 gcaaacaagt acagctccta ttgccgcagt caatgcagcg ataccaggcg cctttggact
     2161 gaaaacattc caagaaaaac atccagatca ttattttgac gtggggatcg ctgaacagca
     2221 ctctatcact ttcgcttctg ctttggcttc tcagggagta cgtccagtcg tcttccaatc
     2281 aagtactttc ttgcaacggg catatgatca attatcacat gatttagcga tcaacgagaa
     2341 tccagtgatt attttagtcg gtggcggaac gatctcagct agtgaccgaa cacaccaagg
     2401 aacatttgac attccttact tatcaagtat cccgaacatc aaatatcttg ctccagcaac
     2461 aaaagaggat atgatctcta tgctagattg ggcgttcgaa caaacagcgc agcctgtagc
     2521 aatccgaatt cctacacatg gtgtagaaca tggtacagct atcgcaaatg attatagtat
     2581 tccagcctat caaacagtaa ataatggagg aaaagtagca attttagctt taggcggttt
     2641 tcttgaatta ggaaaagaaa cagtaaaagc ttttagaaaa aaaggaatcg aagcaacgtt
     2701 gatcaatcca ggaacgatca cagaaaatga cgtagaaaca ttagataaat taaaagaaac
     2761 acacgaatta gtcatcacat tagaagatgg tagtttgtca ggaggatttg gcgaaaatat
     2821 cagtcgttat tatggtgatt cttcaatgaa cgtattaaat tttggtgcag agaaagaatt
     2881 cacggatagc gttcctgtag ggcagcttta tgaacgttat catttaacac caaaacaaat
     2941 cgttgaagat agtctgcgtg tcttgaatat tcaataattc attatgctca ccatccagat
     3001 aattgtttat actagtatct ggatggtttt ttcattataa aaagcaaagg ggtggatgtt
     3061 atgtatcagc aagtagaaga atatgtcaaa aagttccaag aaaaatatcc tagttatcaa
     3121 tcagaaaaat taggtttctt ttcatttggg gatacagaaa aaatggcaga cgagttgagc
     3181 gaactagttg taaaaaagat aaagactggt acatcaagtg gttacgatct gtataaacaa
     3241 gagaatgagc ctttaccaaa agttggtcaa ttagacgtgg tcctgaatgg aaaaaatgaa
     3301 ccagtttgta tcatcaaaaa tataaatgtg accatcttgc cttttagtga agtcacgaaa
     3361 gagcatgctt ggaaagaagg agaaggggat cggacattag cttattggcg gaaagtccat
     3421 gctgagtttt ttaaacatgc ttataaagaa gcgttgaaca tagaatttac agaagagaag
     3481 ctggtcgtat acgaagaatt tgaagtaatt ttcttttagt aagaaaggtc tgctgaaaga
     3541 aatagctcat tattgtctcc tgaatagaaa agggttacat ttttatatga agctgtattt
     3601 ttatataaca ttactggagg agatattgtg gatacactgg aacaaatcaa aggcggagtc
     3661 gtcgtttctt gtcaagcttt ggaaaacgag ccgctacaca gttcttttat tatggggaga
     3721 atggctttgg ctgcaaaaga aggcggagca gttggtattc gagcaaatac gacagaagat
     3781 atcaatgaaa tcaagcgaat cgttgattta ccggtaatag ggattatcaa aagagattac
     3841 gatgggtcag aagtgtttat tactgcgaca gaaaaagaag tggaagaact tttggagacg
     3901 caagcagaaa tcattgcact ggatgcaact tctcgtaaac gtccgacaga aaaaacaact
     3961 gctgaacttg tccaaatgat tcacgaacac ggtcggttag ctatggcaga tatttcgact
     4021 tttgaggaag cgcttcaagc gcaagaagat ggttttgatc ttttgtctac gactttagca
     4081 ggctatacag aatattcacg aaagacgcag acacctgatt ttgatttatt gaatcaaatc
     4141 atagaacaag tcgatatccc agtaattatg gaaggacata ctgactctcc tgaacaagta
     4201 gagaaagcat atgaattagg tgctttttca gtcgtagttg gatctattat cacccgtccg
     4261 caattgatca ctaaaagata tgtcgaagcc gcaagtaaag caaataacta aaaaacacgc
     4321 acggcacaga ttcttgacaa aaaagaaaaa ttgatagata gtttaaagca gattctaaga
     4381 agatgaaaaa agcattactt ttcattttgt cttcaagatc tgctttttaa cgaattttat
     4441 agaaagaagg cgaaaccatg tttgaacgga gtccttcaga attgacagaa cgtgaaaatt
     4501 acaagttgct gattggttca gtcatcccta gaccagtagc agtagtaacg actaaatcag
     4561 caaaagatat agtgaatatc gcacccttca gttacttcaa tatcgtcagt tcgaatcgac
     4621 ccatcttatc cttggcgatc cagagaaaac aagatgcagt aaaagacaca gcaaagaata
     4681 ttcttgagac aagagaagct gttatccata tccttgatga ggacaacgta gaagaagtaa
     4741 ataaaacagc agccaatctg cctaatgagg aaagcgaact gtacctgact gacttgactt
     4801 taactgattc agtaatcatt tcagtaccag cattgacaga agcaaaagtt cgttttgaaa
     4861 catccttata tgaacatgtc gagatacgga ataaaaataa agtaacagct gatttatttt
     4921 tgctagaagt acagaaatat catttaaatg aaacagtcta tgatgataag acaggaagga
     4981 tcgatccaag aaaattgaat gctgtaagcc gtttggcagg aaatgattat gcagcaatag
     5041 gagaaatttt cacgattgca cgtcccgaat aaaaaggagc actagttatg ttaaaaataa
     5101 gctataaacc atcgacagat tcaaaagaaa tgaaaaaaga atatgaaaca gtgaatgatt
     5161 ttctgcaagg gcagtactta gaagttccgc cattgcaaga ccattttgtc gttactactg
     5221 ttactttaga tggtaaggaa atagaaatgc cagatcaaac gattagcgga ttgtttaatt
     5281 actttaacaa ataaatattt attccgaaaa gtgaaaaaac atataaaaaa acacataaaa
     5341 ctaaaaaatt atcaaagaat cctggtgcaa ctgaaagaat ttgcaacggg attctttcaa
     5401 cttatttgtt att
//
LOCUS       DAYYCW010000139         5345 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000139 DAYYCW010000000
VERSION     DAYYCW010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.141"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(100..1119)
                     /locus_tag="ACHAYA_002693"
     CDS             complement(100..1119)
                     /locus_tag="ACHAYA_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8606428.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RADVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1116..1340)
                     /locus_tag="ACHAYA_002694"
     CDS             complement(1116..1340)
                     /locus_tag="ACHAYA_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8606429.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1337..1630)
                     /locus_tag="ACHAYA_002695"
     CDS             complement(1337..1630)
                     /locus_tag="ACHAYA_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606430.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            complement(1667..1804)
                     /locus_tag="ACHAYA_002696"
     CDS             complement(1667..1804)
                     /locus_tag="ACHAYA_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8606431.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(1806..2237)
                     /locus_tag="ACHAYA_002697"
     CDS             complement(1806..2237)
                     /locus_tag="ACHAYA_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606432.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            complement(2251..2745)
                     /locus_tag="ACHAYA_002698"
     CDS             complement(2251..2745)
                     /locus_tag="ACHAYA_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606433.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            complement(2745..3194)
                     /locus_tag="ACHAYA_002699"
     CDS             complement(2745..3194)
                     /locus_tag="ACHAYA_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606434.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(3198..3818)
                     /locus_tag="ACHAYA_002700"
     CDS             complement(3198..3818)
                     /locus_tag="ACHAYA_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606435.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(3818..4726)
                     /locus_tag="ACHAYA_002701"
     CDS             complement(3818..4726)
                     /locus_tag="ACHAYA_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8606436.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(4739..>5345)
                     /locus_tag="ACHAYA_002702"
     CDS             complement(4739..>5345)
                     /locus_tag="ACHAYA_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8606437.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELGMICGKLDRQYYSASAISSIVIPPK"
BASE COUNT         1462 a         1180 c          831 g         1872 t
ORIGIN      
        1 tgggtgatag tgaagatatg cgataacatc actattttat cgaatgattt ttatagttgt
       61 caatatagtt atgtaatgct cctcaacgag gagctatttt tatcgtttag gaatatttaa
      121 ataccaacgt ttgtcatgga aatcttgcgc accgccttta gtgtttccct ctggatcatt
      181 cgttgcccgc atcatgacat agactttctt attagggaag ttacgcatgt taaaagatac
      241 atgatagcca acatttccag aagtattata agcttgattt acatctgctc tataaattcc
      301 atcagctctt actcgagcta attctttccc agtattgtaa tccataatga agatatactc
      361 gtatttatag ttagcaatgt gccatccagc tacatgcaaa tttgcatttt cgatttctcc
      421 aaactgatca atgtgggcgt aattcgttcc atctgtcagt gtaggattag ctgcaccagc
      481 tcgtgttgga tcaatgacag gcttgttttc agaagttgtt ggattttcat cggtaaatcc
      541 atgagctaaa tcatatgcta atttttcttt acttacgccc atttcagaaa gataaccgta
      601 aggatctgta tgatcgcccc agatgttttg tgttacccat aaatgcgatt tgattcccgg
      661 ttggttataa ggagtgtcca acgttaatgg aataccatat ttcattgctg aatctctagc
      721 caattcaacg tatgccttgt agtttttctc aaacgttgct ttatcatgtg tgtgttgtaa
      781 ctcaatctgc acaggactgt tggcattagc atacgaacca gcaccgtact gtacataacc
      841 aggttgaccg acttgataaa caattccgcc gtctcccaca atataagcag tataagcgct
      901 agtccatgaa cgttgcatat actgcgcttc attgcgtcct gttgctgttt cattagccgt
      961 ttcatgcagt aaaatatact gattatttgc tacttgtgag ctaccttcat ttgcgcccaa
     1021 attaaattca ttgttgatag tataggcaaa tccgttaatt ggcaataaaa aaagagccgt
     1081 taacaggctc atcgcagtaa tagtaatttt ctttttcatt tgtttcctcc ttcttcgctt
     1141 tcagccgaga acattttgta ggttcgattt gatacaccca acacactccc taaaaacgcg
     1201 ccaaaaccag taatgatgac aacacagata tctgtgtact gccaattgag cgctttacca
     1261 actaacccca cgaaagtagc tagtgcggga ataattacca gtgcgaacca ttttagtact
     1321 tcgaacgttt tattattcat tttcttctct ccctaaacaa agttttgatt tgttgcgtgt
     1381 gttccaccaa tttttctgta tgtgtatcta atctttcatc gtgtttcttt agttcttcat
     1441 gaatcatcaa tcgatctgat ttgctcgatt ctaaatcttt agtcagcaaa tctaaattgt
     1501 ggcttacttt tgaaagagtc tcagtaattt tcgagaaaga tgcagcaatc ggtctaatta
     1561 ctaataaaat caaagaaacg atagcggtta ttgatcctgc tatcattccc cattccccta
     1621 aattaatcat gtgacaactc ctttacctta aataaaaacg catcaattaa gatgcgctct
     1681 tatctttatt aatgatttta tctgcttctt cgtctgtaat gcataatgga acgaaaacca
     1741 ttacttgttc gttagtgaaa cagccccagt catacattag tttgatatct tcatatgaat
     1801 acatgttact tcccaccttg taattgtttt ttgatttctt cgatttcctt ggtattttgt
     1861 acagaagcaa gcatcgtttt tgaattcaac tgcgctaaac tttctgactt ttctttcaat
     1921 aagacatttt cttgcttgat tgctacattg tttagcattg ctttagaatt tagctgtttc
     1981 aattcatcat tttctgcctt cagcgtttcg tataatgcct taatattagc caattcatct
     2041 acgctatcaa cagcattccc ttttttctct tcttcagtag ctaactcaac ccactgtttt
     2101 ttatcaaaat caaatcttgg tctccagtta ggaactggtg gtttaatctc tgtacagttt
     2161 tcagggatat tttcttgatt gttcaaaatg atttgctcga atccgtaagg cttgattgat
     2221 ttgtaaacta ctttcattag atttcctccc ttaaattgaa taagtgataa caaatgaata
     2281 agctgatcca taacttgaat ttcttctcca tttaatggct ccgtctgcgc caatagatag
     2341 ttgagcactg ttcaaagtag aacggtctat tgacccaacc agttgttcaa aactaattgg
     2401 tgtccgatag ccttctggaa cagttaaaat aaccgaatca ttcccgccac tactttttcc
     2461 gtttaaagcc acaaaatata tagaaactgt ttttccttca cgataaagct ttgctgtccc
     2521 tgtattaccg ttagtgacaa ccagatttac cacttcatat ctgttatcat cagtcgtcaa
     2581 aacacgttta ttattgattg ttacagaacc tgaaaaattc ttatgtcctg caatggcttg
     2641 gtctccatct gtctgcacca atgcttcttt accatcaata gcttctacat gcgtttttaa
     2701 gtatttagca actccgtctt cttttaattg aacaatatca gccattaaac cgtccccact
     2761 ttctcaaatg taatattcgc taatccatca agtttcactt tatcggttgc tgacattaaa
     2821 cctgctgtcg tagttgtagc attacctgga tttttctgtg ctccagctgc aattccatcc
     2881 aacttagttt tatctgtgga cgacatcaac ccatttgctg tagttgtagc tacagctgta
     2941 gttgtggcat ttattccagg atcaccttta tctccctttg gtaaaacaaa attaaatcta
     3001 gctgcagatg atgttcctac attcgtaaca gaagcggttg aaccactaga aacggttcct
     3061 atggtaattg ttgctgcttg gccaggatcg cctttatctc ccttcaccgt tgttggtttg
     3121 ccttctatag cattccaatg agtttgtgga taaacctgta cattgctttg ttttattttt
     3181 acgatatctg tcattctcta tacctcccca attttttcaa aagtaaaatt tggaattctt
     3241 tcgtttgtgt aattttctgc ttgatttaca gcttcttgga atttttgatc tacatatgac
     3301 tgattgacac caccagtccc actaccacct gtagaactaa ttgttccatc ttcatcaata
     3361 gtgatgtttt ctccagcagt ataatttttc agtgaatcga gtttttcttt taactccagc
     3421 gaaaaattga catcagattg tctgactgca gagactttcc ctgtctcatc aatcgtcaat
     3481 aattcaccaa cttttatgcc acccaaacgg tcgatagtgg caactggcaa cacgtagtca
     3541 ccttctccac tgtttaaaat tttttgatac atttctgccg tgataatgcc gtcatcttct
     3601 tcacttgcat agggtaattc tgtgagaaca ttctctaatc ccaaatcttc tttagtaatg
     3661 acgacggctc caatttttcc attaacagat aaaactgttg cttctcctgc tattatttct
     3721 tctaacccaa gaactgcgga agcatgtgtg attggaaaga attggcgttg tacacccgtt
     3781 ttttcatccg tttccatcat tcgtttagtt ttagccatta gatcacccca actttctcaa
     3841 gagtgaacac attttgcttt ggatcatcaa cggttgcgat gatcaaggct ccttcttcaa
     3901 tcgggaatgc tacagttcca actttttcga cttcatgatt atctgaaaag agatcatcct
     3961 gcaaaatatc agtcacttca atctccccgt attcaatcgt gaaaagcgtt gctcgcaatt
     4021 tctgatataa atagtccata tctgccagca aacgctctga aatagacgca tgacgaactc
     4081 cttgaatatc tactcgcgca tccgttagtt cggctaacat gactccacct ggatcaatcg
     4141 tctttaaaat atccttgatt gattcaaacc atgccaaata gtctgattct tgtccttctc
     4201 tccaatcttg gaaactgtgt tcctgttctt gtctccaacg ttcaaattct tctttccttt
     4261 cattcatcca gtccgtaaaa tcgcctttat tttcattaat aaagtccgtc atatccgcaa
     4321 ttaaatcttc gattgattgc caataagaac ccatttctcc ttctgttttt gaaacagcat
     4381 taatgacaaa ataagagaag tcttgagttg ttccaatcaa gtcttctcct ttaaatataa
     4441 tgaaattggc tgtttgtcga tgtaaacact gcatggaata tttatcaaaa atatagttaa
     4501 ttttcccttt tttagcatcc acaatttttg tttctaattg gactggatat ttcccaccta
     4561 caactgattc aaaatatacc ttacattctg ataaatcgta gggaagacca ttttcaacta
     4621 tagtcgcttc cataacctct gtattcttat tgccttgtcg aacttgaatc atccccacgt
     4681 aattataggg ttctgttgta cttaatatga cattccactt tgccattaaa tcacctccct
     4741 attttggtgg tatgacaatg gaagaaattg cacttgcact ataatactgg cgatctaact
     4801 ttccacaaat catacctaac tctgtgtttt gttcataagt ttgcatacga ccattagcta
     4861 agcctcggat aacgccagta tgtccataag ttccatctgc aaaccatgaa cccacttgtc
     4921 cacctcttgc ccaattaata attgcaccaa ctactaattg atcatatcta gggttttgaa
     4981 tcactttcca accaacagca gaccaatcat aggcaatacc aatatcagat gctgcagaag
     5041 tatttcctat cacatgagtt aaaccatatt ttgttccagc acccatgcca caaccaccca
     5101 gatatcctga atattctgca gacaagccat aacactgacc attgccaatt cgttgtccaa
     5161 tcaaagactc taaatgtttt agtcctgctt ctccagtagc tcctcctggt tttaaatctt
     5221 taaatttgtt ataccagtta actgcataat cttgacgttc aggatgtgtc gctgccggac
     5281 gttcatagtt tcgttcaaaa gcatatgctg cttgtcttgg atcagtacag gccttaaatc
     5341 catca
//
LOCUS       DAYYCW010000140         5275 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000140 DAYYCW010000000
VERSION     DAYYCW010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.142"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..263
                     /locus_tag="ACHAYA_002703"
     CDS             <1..263
                     /locus_tag="ACHAYA_002703"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606438.1"
                     /translation="KKKNPAQTSCLFFSNRFAHEINCFSHHRQFIPFTSFNLTATRHK
                     GVIATLSHFAYSSRLRSFVKVKTVVQGAVGIGVKACCSKFLD"
     gene            439..537
                     /locus_tag="ACHAYA_002704"
     CDS             439..537
                     /locus_tag="ACHAYA_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8606439.1"
                     /translation="MYEKFLTLVVAPLFVGLVIELVSHWLDEKDDN"
     gene            complement(691..1188)
                     /locus_tag="ACHAYA_002705"
     CDS             complement(691..1188)
                     /locus_tag="ACHAYA_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606440.1"
                     /translation="MKLFFEKMGWHILPVVIILVGSYSLFCIFRSILEKFEEPKLKRV
                     VFALCFAFLALFVSVGISSYRPDVALSVRPKISKIEQLEAKAEAKQKKIDKISDQLEQ
                     ALKIASQEKLKTFNPKDYPSEVITADWVQKNRDVIATLPDKELKDRYTKRFDDVESYF
                     ELNSQ"
     gene            complement(1216..1671)
                     /locus_tag="ACHAYA_002706"
     CDS             complement(1216..1671)
                     /locus_tag="ACHAYA_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606441.1"
                     /translation="MPLDDFASKQELQKVKQELAEIKQQNQEVIETLKSLDNSVKQGY
                     IKVRGGEDIPKMSELKAEMNRYATYVQKRTAEIDQKLDSYGNISKSVSDELSRQVRPQ
                     VQKFLANDDRATAEELKQNRIANLMSVLASVGLFIGGTAILIVSVLKLL"
     gene            complement(1679..2665)
                     /locus_tag="ACHAYA_002707"
     CDS             complement(1679..2665)
                     /locus_tag="ACHAYA_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8606442.1"
                     /translation="MGATLALKGGKRGASALIGYVNRHGKLDRDRIYTHKLSAENKLA
                     QTQLEATRRTFAKTGGIQAFHWVQSFDNSFDKNNPADVQKAHEIGAKLVDEIAKSYPN
                     HEIYMGTHTDSESGYIHNHIVFASIDTETGKKFHSDTEVGYHLQDLNDKVLLSFGIEQ
                     PKEKFKQVDFEMHAEGKMSNREKMKHMILGAQDMSTDFDSFKKNLQEAYAVEVYEYNK
                     GKRIGFKWVDDEGKDFTIGGRKLGSDFERPAIERTLEFSQQKQEKEVEKPLETKPKVT
                     NDDFSISGLKNLANDIRQQEERKKKEELQRQKEIERQQRSRPFYHDRGGMER"
     gene            complement(2650..3006)
                     /locus_tag="ACHAYA_002708"
     CDS             complement(2650..3006)
                     /locus_tag="ACHAYA_002708"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8606443.1"
                     /translation="MSEQKLNAENRKDSRQIKFRVSEDEYQYLTAIADQTGMSVPQLC
                     KSVALGMKYRAPNVDREGAIQIAKELRKYGGNLNQMARFINMNKGQMPSSSDLALMNQ
                     QLSQIEKGLIDLWEQL"
     gene            complement(3562..3927)
                     /locus_tag="ACHAYA_002709"
     CDS             complement(3562..3927)
                     /locus_tag="ACHAYA_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606444.1"
                     /translation="MAKMDIEDLEKKLAEARHDLSVKIGTNMVEKQGFKSYSAYNTFM
                     RNASKALKSSAGSAPAPAAAKPVATSAEKLPSELKAEAVQFQKQLEKWMEDNWGNKLD
                     YKTFYPELLEFFQKLNSLE"
     gene            complement(3936..4097)
                     /locus_tag="ACHAYA_002710"
     CDS             complement(3936..4097)
                     /locus_tag="ACHAYA_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606445.1"
                     /translation="MTAKTVTIDVEQLEKLLERHSDAIIEYTGGLSEETINQFDLLLT
                     EIIELDSKE"
     gene            complement(4087..4236)
                     /locus_tag="ACHAYA_002711"
     CDS             complement(4087..4236)
                     /locus_tag="ACHAYA_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606446.1"
                     /translation="MKKRLRKKMAKKNSSLDQAMKELMNYAKGERDGFLIHSYDEKGE
                     QKNDG"
     gene            complement(4250..4999)
                     /locus_tag="ACHAYA_002712"
     CDS             complement(4250..4999)
                     /locus_tag="ACHAYA_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8606447.1"
                     /translation="MGQPNKKADIALKDLLSRQDYLVVQGNDLAKAFGGLKSFEQRVL
                     DYCFSFVTKDSKLDDEYEVTALEIIKHFGLNTSGDSYRRIAEAFKKLNENTALYLRVE
                     RPDGTQGLRMTQLFSWIDFYADGLVRFKFSADSAPYVLDLKEKFYSFRLGELSRINGK
                     YALIMLKLWESYRRGKEKHTTITGDLEEWESWFLGKDKRLPAGQFFQKVITRAIEELS
                     DKMSVSFFVTTLKRGRKVVGYEITINDTSKE"
BASE COUNT         1373 a         1195 c          976 g         1731 t
ORIGIN      
        1 aaaaaaaaaa aaatcccgcc caaaccagtt gtctattttt ttccaacagg ttcgcgcatg
       61 aaataaactg cttctcacat catcgacaat ttatcccctt tacctcgttc aatcttacag
      121 ccacaaggca taaaggagtg atcgctacgc tctcacattt tgcttacagc agtcgcctgc
      181 gctccttcgt taaggtcaaa accgttgttc aaggagcggt tgggataggc gtaaaagctt
      241 gttgtagcaa gtttttagac tgatttagat taaatcgtta gtcttgttat acagcgcctt
      301 tacaggcggt cagggcaagc cctaacgacc caaaagcccc atctggtaga ccatcttttt
      361 tttgcaggga gtgaatgcta tattaatctt ggcactaaag ccaactaacg cttacacagg
      421 ttgggaggtg tattccgtat gtacgaaaaa tttctaacgt tggttgtcgc accgctattt
      481 gtcgggctag tcattgagct agtttctcat tggttggacg agaaagacga caactaaggc
      541 ttagtgcctt ccaactcaca cgcctagtcg cagggcgaaa aaaaagccag acaccgtcgc
      601 atggtgtctg gcttttgtgc attccgcact cgaaaaaatt ctaacgtttt cgcatggtta
      661 ttatagcatg gacgttggca aatctcaatt tcactgacta ttcagctcaa aataggattc
      721 tacatcgtca aatcgctttg tgtagcggtc tttcagctct ttatcgggca aagtagcgat
      781 cacgtcacga tttttttgta cccaatccgc cgtaataact tcgcttggat agtcttttgg
      841 gttaaaggtt ttcagctttt cttgactggc aattttcaat gcctgctcca gttggtccga
      901 aattttatca atttttttct gttttgcttc ggctttcgct tctagttgct ctattttgct
      961 gattttaggg cgtacagaca gcgcaacgtc cggacggtat gaagaaatgc cgaccgacac
     1021 aaaaagcgct agaaacgcaa aacagagggc aaaaacgaca cgtttcagct ttggttcctc
     1081 aaatttttct aagatcgagc gaaaaatgca gaataaacta tacgaaccga ccaaaataat
     1141 cacgacagga agaatatgcc accccatttt ttcaaagaac agtttcattt gcgttttaag
     1201 ccccctctcg ttcgattaca ggagctttag gactgaaaca ataagaatag cagtgccacc
     1261 gataaaaagc cccacagagg ctaatacgga cattaaattc gctattcgat tttgtttcag
     1321 ctcttcggca gtcgctcgat catcgttggc taaaaatttt tggacttgtg ggcgcacttg
     1381 ccggctcaat tcgtcagata cagatttgct gatattaccg tagctatcaa gcttttggtc
     1441 gatttcagcg gttctttttt gaacgtaggt ggcgtagcgg ttcatttcag ctttcagctc
     1501 gctcattttg ggaatatctt cgccacctcg caccttgata tagccttgtt ttacgctgtt
     1561 atcaaggctt ttcagcgttt caatgacttc ttgattttgt tgcttgattt ccgctagttc
     1621 ctgcttcact ttttgaagct cctgcttact tgcaaaatca tctaacggca tatcgtcact
     1681 acctttccat gccaccacga tcatgataga acgggcgaga acgttgttga cgctcgattt
     1741 ctttttgtcg ttgcagctcc tcttttttct tgcgttcttc ttgctgacga atatcattag
     1801 caagattttt caaaccgctg atagaaaagt catcattggt gactttaggc ttggtttcaa
     1861 gcggtttttc gacttctttt tcttgttttt gttgagaaaa ttcaagtgtt cgttcgatcg
     1921 cagggcgctc aaaatcagaa cctaattttc gtccgccgat tgtgaaatct ttgccctcat
     1981 catcgaccca tttgaagcca atccttttgc ctttattgta ctcataaacc tcgacagcat
     2041 aggcttcttg aagatttttt ttgaaagaat caaaatcggt actcatatct tgtgcgccca
     2101 ggatcatatg cttcattttt tctcgattac tcattttgcc ctcggcgtgc atttcaaaat
     2161 caacttgttt gaatttttct ttcggctgtt caatgccaaa actcaataaa accttatcgt
     2221 ttaggtcttg taggtggtag ccgacctcgg tatcagagtg aaatttttta ccggtttcag
     2281 tgtcgatact ggcgaaaaca atgtgattat gaatatagcc ggattctgaa tcggtatgag
     2341 tgcccatgta gatttcatga ttgggatagc tttttgcgat ttcatcaacg agtttagcac
     2401 cgatctcatg cgctttttgc acgtccgccg gattgttttt gtcaaacgaa ttatcaaacg
     2461 attgaaccca atgaaaggct tgaataccgc ctgttttggc gaaagtccgt ctagtcgctt
     2521 ctagctgagt ttgagccaat ttattctcag ctgacagttt gtgcgtataa atgcggtcac
     2581 gatcaagctt tccatgccga tttacatagc caatcagcgc agaagcccca cgtttaccgc
     2641 cttttaaagc taaagttgct cccataagtc aatcaacccc ttttcgattt gcgaaagttg
     2701 ctggttcatc aaggctaaat ctgaacttga aggcatttgc cccttattca tgttaataaa
     2761 gcgagccatc tgattgagat ttccgccata tttgcgcaac tctttggcaa tttgtatcgc
     2821 cccctcccga tcaacgttcg gcgctcgata cttcatgcca agtgccacgc tcttgcagag
     2881 ctgtggcaca ctcataccag tttgatccgc aatggcggtc aaatactggt actcgtcttc
     2941 gctcactcga aatttaattt gccgactatc ttttcgattt tcggcattca atttctgctc
     3001 gctcatctca tcactcctca aataaaaatg ctccctattc ggtcgctttt ttcttctcgt
     3061 cattcttcgt ggtcgatatt caaagctaca aaaacgctcc ctattcagtc gccttttttc
     3121 cgcaatgaaa tctccctcgg ttatgccact acaaaatgaa agttccctac tcggtcactt
     3181 ccctttttcc gctctataac cgaatgattt attaacctca tcatactccc tattcggtcg
     3241 cataacaagg ttaataaatc atataatacg caagcaccgc atttggtacc caaatacatc
     3301 gcttgtcgag gtggcgttgc cccctacgaa ctcccccaat cacgcaacaa aaagtagcct
     3361 gttcggctat ttttattacg tgatttcgcc tcagaaaaag tagtctgttc gactattttt
     3421 ctactggctc atgcggtcaa acttgcacgc aagttgacct tatttttttg tctgcccgct
     3481 gttcgcaggc aaacaaaaaa gcaagccaaa aatcggcttg ctcgttcttc aaaaattgat
     3541 cgcatggact agctcgttcc gctattccaa gctgttcaat ttttggaaga actctagcaa
     3601 ctcaggataa aatgttttgt aatccaactt gtttccccag ttgtcttcca tccatttttc
     3661 gagttgcttt tgaaattgca ccgcttcggc tttcagctcg ctaggcaatt tttcggcaga
     3721 agtggccacc ggctttgcag ctgctggcgc aggtgcagat ccggcggacg acttcaatgc
     3781 ctttgacgca ttccgcatga aagtattgta ggcggaatag ctcttgaacc cttgtttttc
     3841 aaccatattt gtaccgattt tgactgaaag atcatgccga gcttcagcta attttttttc
     3901 caaatcttcg atatccattt ttgccatgcg taaactcact ccttagaatc tagttcgata
     3961 atttccgtta gaagaagatc aaattggttg atcgtttctt ctgaaagtcc accggtgtat
     4021 tcaataatag cgtcactatg cctttctaac agcttttcaa gctgttcaac gtcaatcgta
     4081 actgtcttag ccgtcatttt tttgttctcc tttttcgtca taactatgaa tcaaaaagcc
     4141 gtcacgttcg ccttttgcat agttcattag ttctttcata gcttgatcta gcgaagaatt
     4201 tttctttgcc atctttttac gcagtctttt tttcaactaa atcactcctc tattctttgc
     4261 tggtgtcatt gatcgtgatt tcatatccaa cgacttttcg cccacgcttt aaggttgtga
     4321 cgaaaaatga aactgacatc ttatcagata attcttcaat agcacgagta attacttttt
     4381 gaaaaaattg acctgcagga aggcgtttgt ctttaccaag aaaccaagat tcccattctt
     4441 ccaaatcacc tgtaatcgtt gtgtgttttt cttttcctct acggtaggat tcccagagtt
     4501 ttaacattat gagagcatat ttcccattga tccgagataa ttcacctaaa cgaaagctat
     4561 aaaacttttc tttgagatca agaacgtatg gagctgaatc agcagaaaat ttgaatctta
     4621 ctaacccgtc agcataaaaa tcaatccaag aaaataattg cgtcattctg agaccttgag
     4681 taccatcggg gcgctcgaca cgaagataaa gcgcagtatt ttcattaagc tttttgaaag
     4741 cttcagctat tcgacggtaa gaatctccag aggtgttcaa cccaaaatgt ttgataattt
     4801 ctaatgcggt tacctcgtat tcgtcatcta gttttgaatc tttagttaca aagctaaaac
     4861 agtagtctaa aactcgttgc tcaaacgatt taagccctcc aaatgcctta gctaagtcgt
     4921 ttccttgcac tactaaataa tcttgacggc ttagtaagtc ttttaatgca atatcagctt
     4981 ttttatttgg ttgtcccaaa ctgaaaatcc tccttttgta ctttttttaa aataatacca
     5041 tttattcagt ttttttgcaa tatttgaatt ttcaacgtgg aaaatagtat ttttgacgtg
     5101 gaaaatagta tttttgacgt ggaaaatagt attttcaacg tggaaaatag tatttatagg
     5161 cgctacaaac gctggtataa agccattcct tttcccctaa agtattttaa agtatttaaa
     5221 agtaatctat aaagtattct ctttaagcgc gcgggtgaga aaaaatttta aattc
//
LOCUS       DAYYCW010000141         5243 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000141 DAYYCW010000000
VERSION     DAYYCW010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.143"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..994)
                     /locus_tag="ACHAYA_002713"
     CDS             complement(<1..994)
                     /locus_tag="ACHAYA_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8606448.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQ"
     gene            complement(1065..1361)
                     /locus_tag="ACHAYA_002714"
     CDS             complement(1065..1361)
                     /locus_tag="ACHAYA_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606449.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            complement(1354..1647)
                     /locus_tag="ACHAYA_002715"
     CDS             complement(1354..1647)
                     /locus_tag="ACHAYA_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFM8606450.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1793..2344)
                     /locus_tag="ACHAYA_002716"
     CDS             complement(1793..2344)
                     /locus_tag="ACHAYA_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFM8606451.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2518..2817)
                     /locus_tag="ACHAYA_002717"
     CDS             complement(2518..2817)
                     /locus_tag="ACHAYA_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFM8606452.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            complement(2844..3152)
                     /locus_tag="ACHAYA_002718"
     CDS             complement(2844..3152)
                     /locus_tag="ACHAYA_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8606453.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            complement(3269..4228)
                     /locus_tag="ACHAYA_002719"
     CDS             complement(3269..4228)
                     /locus_tag="ACHAYA_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8606454.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            4372..5148
                     /locus_tag="ACHAYA_002720"
     CDS             4372..5148
                     /locus_tag="ACHAYA_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8606455.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
BASE COUNT         1566 a          968 c          816 g         1893 t
ORIGIN      
        1 tttgaaaatt gttagtcatt ccaatacgtt gagaaattct tccatctgta taaacacgga
       61 attgttttcc accagccatt gttgtatttc cattatcatc cgttacaata ttaattgaat
      121 agtagcttcc attttcatct tgcattagat ctttcatata ttcatttgtg ctaattaaat
      181 tagtatttga tgcattttta tcaggagaat catacaacca taaagcttta ttatttagta
      241 cagtttcttt agcagcttcc caacttgaaa ttgctgagct acttgtagat tgtgtcgttg
      301 tagttgttgc ttgttgagta gattcttcta ctactggagt ttgaggagtt tgtccattca
      361 caatatcaat gaaaccttgt tttaactgtt ggttctgagt ttcattgaag tacatgtagt
      421 ttttatcatt gccttgattt tgttgcgtta aataaacttt aggttgtcca ttttgaaagc
      481 caaagaaata tacatgctgc cctaaatatt ctccagtttc tacatcagaa taaactgcta
      541 ctaaattgaa atctgcatga ccagttccat cttctgacca ttgtacggta actggtgctt
      601 cattgattgc cattttccaa tttccatcaa taactgcact tggtaaaggc gtaccataca
      661 gacttacatt gttctgagga ttgtagcttt tatattcttg acctaatgtc tttccccatt
      721 gtgggacaaa aattgctagt tgagtagatt tctcactatc ccacaaagta tttttaacct
      781 cttcagaaga agattcaatg attgaacttt cacttgtact tgcttccaca gattgtttca
      841 tttctgttgt agtttgttca gttgattgcg ttttactttt tttagcctca attgttgtat
      901 cagtttctac agtctcttta gtcctcgtat tctcagatgt acaaccagtg aataaaatcc
      961 ccattccaat tagtaataac aaccattttt tcattttatt tccccctttc aatttctccc
     1021 tcgcgtactt attttatcag tcaattaatc tcaatggtgt tcatttagtt aatcctttcc
     1081 caagaatgtc gatagccagc atgacaacga tctcttcctt gagggctttc agatggatta
     1141 aaactagtag aaatttccct tccacatcct ctacagcgat aagttctcat tatttttggg
     1201 ctattagtat tatttttctt agttagagct atgagtccaa ctatgattac cccaactgtc
     1261 ttaattactt ttcctttatc tatattcgga tgcgcaagca tatatttata taaccaatat
     1321 ccaccgtaaa ttaaaccacc taatacaatc agcttagcca tgtactcgtc tcctttcttt
     1381 ttgtttaatt actttttgaa tccatcttgc agagaaacca taaacttctg tcaattgacg
     1441 gatatctaca gttttttcct gttctatcat gttttcgatt ctttttctaa ctaactcacg
     1501 atcgtacatt ttcattggaa aagaaatctg ttgccctcga aaagcaagat aaaattgata
     1561 gagcaattct gaatccagtt ccatttcctc aaaaaagtca ttcatacttt tataagagtt
     1621 actcaaacaa gtttcatcaa tctccatcta cacaccccct gttatttttt acataatgaa
     1681 aatagcatat tactgggtac attgataatt catataacat gtatcatcac atgtattttt
     1741 aaagtcaaaa agcatgtaaa actacttata agtaacataa gaaaataaaa tgttattttt
     1801 cttgaaataa taattgacta tctgccaatt gccattcatt acgaacatga atacttagtg
     1861 ggtgggataa cttaccttct gcatttatca atactttggt catctttttt tgtttcgcac
     1921 tcaattgatc gtaaatggtc ttcgcttgtt cataagccac ttgactcgtc gctgtaatcg
     1981 aaccacaagc aaaaataatg gtgttgattt tcttttctgt gaaaacagtt gttaatattt
     2041 ccaaagattg ttcatcattt ccacgaggaa atacccgagg acttttcgct tttgtatacg
     2101 aaaaaagatt cacagcaata aaaccagtaa atcctaattg tcggatattc ttttcgatta
     2161 ataacatact caaatcattc gtaaatggtt ctgtatctgt tggacgaata gtaatgacta
     2221 ccgctaattt atcttccgct tcttttcctt tgatcgccca ttgtcgtttg aaaacatagc
     2281 gatgggtatg atgaggatct cgccaaactt ctgtttgtgt ggtcactaat tctttttcaa
     2341 tcatctcttt ttctcctatg ctaatttttg ttcaagcaat tcaatcacca ttgccgttat
     2401 aagtgtcaat actagttgaa aatatttcat attctttctc cttttttctt cagaatttga
     2461 tgaatcgctg taaggatatc ttctaaacat ttcgcaatca ttctcaaata atcgatctta
     2521 ttcattcgtt cctccaatct gactccaaac caatcgtcct tctaccagta acccacgaac
     2581 aaattctcgc tggctggtaa tatctgtaat gaaataaact acatagtctt ttccatcttc
     2641 tggtgcgcaa taaatataaa gatgtgtcat cactaaacgt ggtagagtaa catcttcagg
     2701 aagcataccc atttcaattt cctctttttc catcgtaacg gattcatagc cccactcttc
     2761 aggagtagtg actagctgtg cacacttttc cagtacttcg atagccgttt ctaacaagtc
     2821 ctttcgttcc tttcttttag atatcagtat tcttctggta ataacatcgt ttgatactca
     2881 cccgaatcaa tgacccatat tttttgagac actgatttca aacgaagctt aggtgaaatc
     2941 agatactctt ttcttgcttc tggttcttct tgacgatgaa ccaacttttg atttcctttt
     3001 tccgtagaaa attcaaagat ttgaagatag tctaaatgaa attgcttttg aacgttttga
     3061 tcgattagat tccaacataa agcttgtaat tccaccggca aactgtccat tactcccttt
     3121 gtgacataac gttgtccttt attttgaaac atttcttttc cctcctttgc cataaaaata
     3181 gcccaatctt ctattaaaga agattgggct tcatggatat aatatatatt tgatattcaa
     3241 tttgattcat tttatactat ctgatttgtc aaattaagct agacaatatg tctttatcta
     3301 tgcagctcaa aaatacttcc aatggtgttc gataatttaa tgattttctt ggaatatggt
     3361 ttcttttcga ggcaacggat tgaataaagg tttcatccgt gtccctaaag tccattgttt
     3421 taggtaagcc atctcgtcta agcaagccgt ttgagtgctc atttagccct ctttgcgaag
     3481 gtgttcctgg atctgcgaaa aagatggcaa tatcgttcga gttacttata gatcgccagt
     3541 tggaaaattc ttttccacaa tcaaatgtca tggatttaaa caagtgttta ggcatggatt
     3601 tgaaccaatc atttaacctg ttttcgatat cttcggctcg tctgcctgtc ggtttcaaag
     3661 taataataac cttggataaa cactcaacca atgtaataac agcgctcttg tgtttaactc
     3721 ccacaatggt gtcgacttcg agatgaccaa attctttagt caggtgtgga tagagactgt
     3781 tccgttcatg aatcgttctg cgaaaggcct gttttccacg cttttcttga tgattattcg
     3841 gtttacgttt ccccttcata ggtaacgact gtgtgtcaaa aaggcctttt ttaaacatcc
     3901 gatagagtgt acgaacagaa caatcaattg gaaactcgcc acggccgata atcacatcgg
     3961 gtgaccatcc ttgagctacc ttttcttgaa catatgttga ttgttcatca gataaaacgg
     4021 ttgaacgtcg tccacaacga gatttatttt gtttgtattg acggtaataa tctagcactt
     4081 tttttccttg tttgagaaac gaataaacat tataaatggt ttgtctagag cgatttaaac
     4141 tttgtgcgac aagagcgaca ggtttctcga tattgaaata agactctatg attactagtt
     4201 cgtctgttgt aagatgggtg taagtcattt gtactcactc cttattaatt ctttggtcgg
     4261 aactattttg agtgtagcac aaatgatctt tttagttgtc tagcttaatt ttacaatcga
     4321 cgttttaata aattgtgata taatgaagta taacttttta gggagtgtac tatgaatttt
     4381 caagataata gaccattata tttacaagta gaagcttcta tacgtaacga tattcgtgaa
     4441 aaaaaatatc ttccaggaga gcaattacct acagaagaag aattatgtaa aatttatgga
     4501 gtaagtaaga ttaccattcg aaaagctttt aaacttttag cagaaagtgg aacagttgaa
     4561 cgattaaggg ggaaagggac ctttgtaact caaaaaaaag aaagtttaaa attagctgga
     4621 gttcatggat ttagcgattc tctttctacc cgaggacata aaattagata tactgtttta
     4681 cattctaaaa ttataaaggc tgatgagttt ttagctaata aattacaaat caatgtaact
     4741 gatagagttt acaatattaa acgtttaatg tgggaagacg gtgccccgat gggaattgat
     4801 aattttttcg catcagaaga aaaatttcca actttatttg aaaaaggtag ccaatatact
     4861 tcattatata aacttctaga agatgactat gatgtcaaag ctacgagctc tattatggaa
     4921 ataaacggtt tatctgcaac acctgagctt tccgacttat tgcaatgtat agtaggagat
     4981 ccgctattta ttttagaaaa ggttggcaaa gataaaaata atcaaccatt tcattattct
     5041 caatccacag ttcgttgtga tagaattagt tatgttattg aagtcacaga aaatatcact
     5101 ttagatacgg tgcataaaaa tgattcagat cctaaagtaa tagattgata ttaaaagaaa
     5161 tcatacccaa agcaatcaat tttgattaga tttgggcatg atttttattt ttactaatat
     5221 tctctaaata catcaaaaaa ctt
//
LOCUS       DAYYCW010000142         4995 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000142 DAYYCW010000000
VERSION     DAYYCW010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.144"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="ACHAYA_002721"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="ACHAYA_002721"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM8606456.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="ACHAYA_002722"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="ACHAYA_002722"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM8606457.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="ACHAYA_002723"
     CDS             2591..3376
                     /locus_tag="ACHAYA_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8606458.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="ACHAYA_002724"
     CDS             3389..4240
                     /locus_tag="ACHAYA_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8606459.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="ACHAYA_002725"
     CDS             complement(4364..4816)
                     /locus_tag="ACHAYA_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8606460.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1690 a          817 c         1039 g         1449 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctga
//
LOCUS       DAYYCW010000143         4974 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000143 DAYYCW010000000
VERSION     DAYYCW010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.145"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..754
                     /locus_tag="ACHAYA_002726"
     CDS             68..754
                     /locus_tag="ACHAYA_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM8606461.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            756..1700
                     /locus_tag="ACHAYA_002727"
     CDS             756..1700
                     /locus_tag="ACHAYA_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM8606462.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1899..2543
                     /gene="pcp"
                     /locus_tag="ACHAYA_002728"
     CDS             1899..2543
                     /gene="pcp"
                     /locus_tag="ACHAYA_002728"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM8606463.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2622..3899
                     /locus_tag="ACHAYA_002729"
     CDS             2622..3899
                     /locus_tag="ACHAYA_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606464.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(4083..4832)
                     /gene="pnuC"
                     /locus_tag="ACHAYA_002730"
     CDS             complement(4083..4832)
                     /gene="pnuC"
                     /locus_tag="ACHAYA_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM8606465.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         1600 a          871 c         1061 g         1442 t
ORIGIN      
        1 caactatatt ttttatgtgt tttgctttat aattaaaaaa atattgtcat tttatggagg
       61 aaaatatatg gaaaattatc ttagcctttt aggcgtcttg attgttattc tcgggtttgc
      121 atttaaatta gactcgatat tgatcgtaat ggttgcttta gttgttacag ctttgactgg
      181 cggtcttggc attcaaggaa tgttagaact tttaggtaca agttttgtaa acaatcgcgg
      241 gatggcgatt tttatcatca ttatgttggc aacaggaacg cttgaaagaa atggtctgaa
      301 agaatcagcg gcttccttga tcaagcgatt caagaaagta tctgctggta tgattgtaga
      361 tatttatggg atctttcgaa tgatctttgc ggcttttaac gtgagttttg gtggagttgc
      421 tggatttgtt cgtccaatca ttttaccgat ggcattgggg accatcgaat caaaagggtt
      481 agaagtccat cctaagcatg aagaagaact aaaaggtatg gcttcagcta tggaaaatgt
      541 ctgctggttc tttggccaag tactattcat cggaggagca ggggcgttgc tagtccaatc
      601 aacactgaaa gatctaggat atgacgtaac attgggacag cttgctttag tggaagttcc
      661 agtggctatc acggctttag ttgtggcaag tgtctacttc tatcttaaag aaaagaaatt
      721 agcgaagaaa tattatccag gaaaggggca ataagatgga cttttttaca aatcctgata
      781 ttttattagg cgataaatta ctggagatcc tttatatcgt gatgggactc gtattgattt
      841 acactggagt aagaaattta acagataaaa ccaatcctca tcgttatggg tcagcctttt
      901 tctggacggt cttaggggta gttattgcag gaggaagatg gcttccggca atcgtgaacg
      961 gtggcttgat ctttgcgatg accataccag caattgctaa gaaagtcagc aaaggagaaa
     1021 gccggcttcc atcaaaagaa tatatggaaa aaatgtcaga caaactagga atgaaaattt
     1081 ttattccagc tttgagtatc ggtgtctttg cgatcttgtt tgctctattt accaatttag
     1141 gtgctttagt cggcgtgggc gtcggtgttt ttgcagccat gctaatcttg atgttctttt
     1201 ctcgagataa tcgaccaagt acatttttag atgatgcagc agatatgctg ggcacagtag
     1261 ggccgttaag catgttgcca atgcttttag cttctctagg tgcggtattt acaagcgctg
     1321 gagttgggac agtgatttcc aatgcagtag gtacgatcgt tccagaagga aatgtgaaca
     1381 tcggcatcat catttacgct cttggcatgg tgatatttac ggtaatcatg gggaatgcat
     1441 ttgcggccat caccgtgatg acggttggga tcggtgcacc attcgtattt agtcatggag
     1501 cgaatccagc gttaatagga atggttgcat tgacttgcgg tttttgtggg accctgctga
     1561 caccgatggc tgctaacttc aatatcgtgc ctgtagcgat gctggagatg aaagacaaat
     1621 acggagtgat caaaaaccag ttgtttattg cgttatttat gctagttttc caaattgctt
     1681 atatgattct atttaaataa tagaagagag gggttgtgac agaataattg tcgcaacctc
     1741 ttctaacatt gtagtggaac actatttttt tataagaata tattaagttt gatctgttat
     1801 gttatttaaa ctattgtttc ctttatttac gctgttcaag gaaaatggta tgataaaaga
     1861 taaccatatg ttaaatcaat cggacggagg aacaacagat gaaagtacta gtaacaggtt
     1921 ttgatccatt tggtggggac aaagtgaatc cagcctatga agcagtaaaa aaaatgccag
     1981 atgaaatcag cggtgctgaa atcatcaaag tggaagtacc gaccgtattc gaaaaaagca
     2041 gtcaagtagt caaagaagcc atccaacagc accaaccaga tgtcgtgatc tgtgtcggac
     2101 aagcaggtgg gcggtcagca gtctcgttcg aacgtgtcgc aatcaatcta gcagaagcaa
     2161 gaatccctga taatgaagga aatcagccat tcgatacggc tttagaagaa aacggtccag
     2221 ccgcttactt tacctcattg ccaatcaaag caatgacaaa aaatgtacaa gatcatggtc
     2281 taccagcgta catctcttat acagcaggaa catttgtctg caatgatatc atgtatcgtt
     2341 tgcttcattt gatcgaaaca caattcccgg caattcgagg tgggttcatc cacgtgccat
     2401 tctcacccga tcaagtgatc gatcgtccag tgggtacgcc ttctatgtct ttagaagaca
     2461 ttgcttcttc tttgacttat gcagttgaag cagcagtaga aaataaagaa gatatttccg
     2521 gaaatgccgg aacaactcac taatttagca agaaacactg gctggttgct aacgttatgt
     2581 tagtagccag ctttttgtaa ttagaaagga aagaaacagc cgtggaagaa tggcaatccg
     2641 tctttgaaga atggtttcca aaagaaatca gcaaaagcta tcccatcaaa atttctaaac
     2701 aatacacaag cagtcaacgt tgggagatct atgcaaaatt aacgaaaaaa cagcgggaac
     2761 tagtcgataa gcaccgccga tatctaatca gctctcgttt tatggaagaa cattatttag
     2821 ctgcaacaga ttgggtgttt tctgacttca agatcaatcc gttttttcga acaaaacgaa
     2881 gccagcaaaa attgtattgt gagtgtggta gagaattaaa agtgcaatac atcgttaaat
     2941 cgccaaaaac cggaaagatc ctaaaactag gcatcaacca ctttgccgat cacctgcatg
     3001 tctctccgac agtggcagct tcgatccatc agggaatgac aaaagttgat ctggccttag
     3061 acgaattatt atggcttaag cagaagaata tcgattttcc tgaaggacta tggcaaaaat
     3121 actgttttgt cttgtaccaa aatcgtcgga tgaaacagcc atatttaccg gatataaaat
     3181 tagctcagcg gctcgcagaa tttcgccaag tagaaatgcc gatttacata gcggattatc
     3241 aagcattgga aaatgaaatc aagaaaataa gtgaacacat caatgggcag tcaaaaaagc
     3301 gacagataaa aaaagaacta tttgacgact ttgcagaaga attagtaaag gatgtagaag
     3361 aattcttgat caactatcgc gcatttttac gtaaagattg gcaatccatc gtatatgaag
     3421 aagtgccagt acatcctaat gcttattttg aaacatttat ttccgtgttg agaaagacaa
     3481 aacgtcaacg aacaccagaa gtcactgctc agatggaata ttttgcgaaa aaccaacgat
     3541 tcatccaacc aaaaatctat ctatttattt ggaagcaata ttgtcgctat ggttttacag
     3601 aagggttctt cgacagcatt cctcggatag tcagaaatgg ttttttaaaa gtattgcgta
     3661 aagaacgaga agctatacaa tctgctgata agaaagaccg tacagtctca aaagaaaaat
     3721 ggcaactagt agtgaaagat atccaatctg gaaatgtgca agagacaatc gacaaatgga
     3781 aaggaaaaca ttatcgtttt actgaagctc agaaacaagc gttggaatat tatcagaagt
     3841 tagaagaatc actgcgtttc aatgatgagg caagaaagta tctgaaggaa ttactataaa
     3901 aaatagaggc tgggacagaa gcgtttaact ccaagaaata agaagaaatt cacgaaaatt
     3961 gcttttcaaa tttttgtgaa tttcagctta tttccgaagg agttgcttct gattccgccg
     4021 tttatacgtg tttagagcgt gagacaaaag tgttctttac ttttgtccca cgctcagttt
     4081 ttttatgcgt gttgttgtgc gttgtagtat tcttcttcca ttttttgcat acgttcacgt
     4141 tctttttggt tataccaagg tgaaacggta aatgcaagga catcattcag caagtaaaca
     4201 gagctgttca cgaacatcgc caaacttgca gatccatgcg taaatgaaat ataccaaagg
     4261 accatttgag caagccctga agctaaccac cagtaatatt ggttgttgta acgcaagaaa
     4321 ctgatgatac cagcagtcaa gctaatagaa aaagcaatgg catctaccca cggccgagga
     4381 tcatctgtat aacgaccaat caggaatcct gaaacaagat agacaaggaa agtactgatg
     4441 attgcgataa accagctttt tccagtgaat tttcggattt ttgaagccat attatgattc
     4501 cattctttac ttaacaatac ggggatatcc aatgtgatca tataagcaat ctgttcaccg
     4561 atacttaaat agttctttgc tgaaaaccca acgataataa aacaaatcgc agacaaaata
     4621 cctaaaatac cgttgactga ttttgccgca ttgatggata aaatacacaa tacaccaagc
     4681 gtggtaccga taaatgtaat aacggacagc caagtgatgg gactattgac aagagtcatg
     4741 acctggcagc caagactgaa aaagaaaaga taataatttt gttgcggcca gccttttagc
     4801 tgatgagcta aaaactgaaa atatgacttc atgtatgagg actccttaag acactaaatg
     4861 tagtgtgttc tgtatttact aacacaatct attgtatgct tttcttgaaa aaaaactagt
     4921 ctttcaatta taaataattt gtgttatgat aattttctgt ttgttttctt gaaa
//
LOCUS       DAYYCW010000144         4933 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000144 DAYYCW010000000
VERSION     DAYYCW010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.146"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACHAYA_002731"
     CDS             85..972
                     /locus_tag="ACHAYA_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8606466.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACHAYA_002732"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACHAYA_002732"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM8606467.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACHAYA_002733"
     CDS             2274..2720
                     /locus_tag="ACHAYA_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8606468.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACHAYA_002734"
     CDS             2993..4345
                     /locus_tag="ACHAYA_002734"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM8606469.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACHAYA_002735"
     CDS             4516..4803
                     /locus_tag="ACHAYA_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8606470.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAYYCW010000145         4884 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000145 DAYYCW010000000
VERSION     DAYYCW010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.147"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(426..1361)
                     /gene="murB"
                     /locus_tag="ACHAYA_002736"
     CDS             complement(426..1361)
                     /gene="murB"
                     /locus_tag="ACHAYA_002736"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM8606471.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1434..2354)
                     /locus_tag="ACHAYA_002737"
     CDS             complement(1434..2354)
                     /locus_tag="ACHAYA_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8606472.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2467..3219
                     /locus_tag="ACHAYA_002738"
     CDS             2467..3219
                     /locus_tag="ACHAYA_002738"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM8606473.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            4083..4616
                     /locus_tag="ACHAYA_002739"
     CDS             4083..4616
                     /locus_tag="ACHAYA_002739"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM8606474.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1446 a          952 c          844 g         1642 t
ORIGIN      
        1 aatttagaca aacctaatgt ttcatataac ttaggcagca ctacataatt ctttttctca
       61 aattctaaat atctagctag cagcagtttt tctctatctt cctcttctat aaggtaatcc
      121 aacttcatat cctacacctc aaaaaatgcc tcataccaat tacttgatat gcagatgaaa
      181 gttcatcttt tcacctatac tgattttatg atggattatg cttgtttttt catcagttat
      241 tctcattttt tatctttttt ttgagaatat tgcaaaaaat ggaaaaattt atgaggaaat
      301 attctatatt ttttgtttta ttgtgttaaa atgtacaaga tttgcgccat ttgcgtaaac
      361 aaccacaaaa aagagcagga cggccaccgt cctgctcttt tacttatttt aagttccatt
      421 acttattaat tggctaaact tctgcttgtt tctgatattg cttgtttttc tccaatgatc
      481 cgcacttctg tttctaaaga cacatcaaat ttttctttga ttacttcttg gatgtgcgca
      541 atcaattcaa tataatctgt cgctgtcgca tgatcgatat tgacgatgaa tccagcatgt
      601 ttttcagaga cttgtgcacc gccccatttc aggccttgca atcctgcttg ttggatcagc
      661 tgacctgtat agtgtcctac tgggcgctta aagacacttc cgcatgaagg gtattctaat
      721 ggctgttttg attgacgcaa ttcggtcaac tcatccattc ttgctttgat tgcttcataa
      781 tcccctgttt gcaaactgaa tcgagcttcc aatacgatag cacgtaattc ttggacttta
      841 ctgtgacgat aagaaaatac catttcgtct tttgtcaaag ttttcaatgt tccatcttca
      901 agcaaaagat ccacttcagc aaaaacatct ttgatctctc cgccatacgc acctgcattc
      961 atataaaccg ctccaccgac acttcccggg atgccgcaag caaattcaaa tccagtcaat
     1021 gattctgcca aagctgcata agtcgtatca atcagtgagg cacctgcttc cgcaatgacc
     1081 atcgttcctt tgacatgaat ctgtttcatt tcagtcagca tgatgactac accgcgaatc
     1141 ccgccatctt gaacgatcag attgcttgca tttcctaata ccatccaagg tatatcatgg
     1201 attcggcaat aatcgatgat ttgctttact tcttcttgtt ttttcgggaa tgcaaggaca
     1261 tcagctggtc cacctgtttt cgtaaacgta tagttcatca atggttcatc aaacaatata
     1321 gtaatcgtgg ctaagctttc aactatttct tctttattca ctgaggattt tcctttcttt
     1381 tctcaaacgt cctcattatt ctatcacgct taatacaatc ttgccagtct tgttcaaagt
     1441 tgatgacggt agtgatcttt aagcacttcc tcacataatt catgacttct tacaacttct
     1501 ttttgtttaa gcaattctgt ttccgattta cctaaacgga tctgagaaaa gaaggcctct
     1561 accattggct caaacccgcg tttgactagc gtgtttgtcc agtcgccaaa ttctttcact
     1621 ttttttccat cagcatcttg aatgacaagc tgggtcagat cgtttacttc ataagtacca
     1681 tttttacttg ttacttgata tttttctata ttagcgccac ttgctaaatc catcgttaaa
     1741 atagccgttt gattttgtgt ttctaattgt aaaatggcat attccaaaaa ttcctttgtt
     1801 tctcttatct ggtattttgt tctcaaaatg ggttcatcta ataaatacac agctgtatca
     1861 ataagatgta aaaacaaatc ataaaggacg aactctgtcg tttcttttgc cgctattcga
     1921 tttttttgca ggtggatcaa ccgcttatct gggatacgtt tcaattcttc taccatagga
     1981 gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt tcttatcagc taattcttgc
     2041 aattcacgaa cttcatttag atggacactc agtggtttgt caatataaac atggatacca
     2101 ttttctagac attttttggc taattcataa tgtacttttg ttgctgcgtg gatcatacat
     2161 gcttcgattt tttcttctaa aagctcatcg atcgtttcat atagatgttg gaacccgtac
     2221 tctttttgaa ttctttcttt tgtttcttta tttcttgttg caaaataaaa atcaagtgtt
     2281 ccttgttttt tcgcataagt tggtaaatat gctttttctg cgatattccc tacaccgatt
     2341 acaccaatct tcatctactt tcctcctgga tcatttagtt actttcatct tacaccattt
     2401 ttttctaaaa tgaggtacaa tagtatcgca aaggtaagga aattaattcg aaggagtgtg
     2461 cttttattga aatttatctc ttggaacgta aacgggcttc gtgccatcgt aaataaaaat
     2521 tttttagaag tttttcatga gttagacgcc gattttttct gtctgcagga aacgaaactc
     2581 caagcaggac aaattgattt agatcttcca ggttattatc aatattggaa ttacgcggaa
     2641 cgaaaaggct attccggcac agcgattttt gctaaaaaac cagcattgaa tgccacttac
     2701 ggaatgggta tcgatatcca tgatacagaa ggccgtctga ttactttgga atattccgat
     2761 ttctttcttg taacctgtta cacgccaaat tctcaaagcg aattaaaacg tttagattac
     2821 cgattagaat gggaagaagc cttttataat tatttggaaa atctaaaaaa acaaaaacca
     2881 gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga ttgatttgaa aaactggaaa
     2941 acgaatcaaa aaaatgctgg attcactcca gaagaaagag cggctctttc tcgactgttg
     3001 gataacgggt ttatcgatac attccgttat ttttatccaa cccaggaagg tgtctactct
     3061 tggtggaact accgattcaa ttcaaggaaa aacaatgctg gttggcggat cgattacttt
     3121 ttgacgagca aagatttaga gccaggacta gctgatgcaa agatccatac ggacattatg
     3181 ggcagtgatc attgtccagt agaattagat ttgaaataaa gaggttgtga aaaagtcgtt
     3241 tagtttcgag tattaagaaa aattttgtga aaatggcttt ccacattttg cataaatttg
     3301 gcataatacc gagaaactgg cttttgaaca ctgtttatcg agagattgtg aaaaagctgt
     3361 cctgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatatttt tcgtttggtt
     3421 gggcttatta ccgcagatac aagcttttga acaccgttta ttaagaggtt gtgaaaaagc
     3481 tgcactgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat ttttcgtttg
     3541 gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttattaagag gttgtgaaaa
     3601 agctgcactg catcaagtaa taagaacagc caaacaaaaa tagttcctca tatttttcgt
     3661 ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttattga gaagttgtga
     3721 aatcagcgtc ctgacctgag tattagagaa acaaatggca aaatagttcc tcatattttg
     3781 ctattttttg agctaatacc gagggtcagc tgattgaaca ccgtttagtg gagaagttgt
     3841 gaaaaagtcg cctactttcg agtcgtaaga aatttttttg aaaatggttc ctcacaactt
     3901 tgcataaatt tgacttaata ccgagaagtc atgatcccca tctaactcca aatcacaagg
     3961 aatgagttca aaaactagct tttctcaaca aatattacat aaaaaattct atgttttttg
     4021 ttaaatttat ttgctttttt caaaagtatg ttacagtaat ttcccaagga ggttttacag
     4081 aaatgaattt tgttgccatg gattttgaaa ctgccaatca tcagccttat agtgcttgtt
     4141 ctcttgcgct agtcatggta aaaaatagcc agatcgttga tgaattctat acattgatac
     4201 agcctgagac tccttttttc tggcgaaatg tccaaattca cggaatccat caagaagacg
     4261 tacgcaatgc gccgaaattc ccagaagtct ggcaaactat ccaaccatat tttcaaaaaa
     4321 atcgtttagt cgttgcacat aatgctgctt ttgatacaaa ggtcttagcg ggatgcttag
     4381 attattatca tcttgaacag cctaattatc tttctttatg tacagtgaaa agtagccgtc
     4441 gcctgtttcc tgaatttcca aatcatcgat tgaacactgt gtgcgaaaat ttgaacatcc
     4501 aattagatca tcaccatgat gcattggaag atagtcgtgc atgtgcggaa atcctgcttt
     4561 atcaagaaaa aaaattcggg acagatccat tgaaaaaact tgtgactata aagtaaaaga
     4621 atacttatga aacacacaaa gaggtcgtga gcctgccgtt tcactccgag tcacaaggag
     4681 caatttcaaa aaatagcttc tcatgtttat taaatattgt gactggtgac cgaggagctg
     4741 gcaggagaac accgtttatt ggagaggttg tgaaaaagct gttctgcatc aagtaataag
     4801 aacagccaaa caaaaatagc ttctcatatt tttcatttgg ttggacttat taccgcagat
     4861 gcaagctttt ggacaccgtt tacc
//
LOCUS       DAYYCW010000146         4840 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000146 DAYYCW010000000
VERSION     DAYYCW010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.148"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHAYA_002740"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHAYA_002740"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM8606475.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACHAYA_002741"
     CDS             complement(1297..2133)
                     /locus_tag="ACHAYA_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8606476.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACHAYA_002742"
     CDS             complement(2317..3534)
                     /locus_tag="ACHAYA_002742"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8606477.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACHAYA_002743"
     CDS             complement(3683..3844)
                     /locus_tag="ACHAYA_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606478.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACHAYA_002744"
     tRNA            3917..4000
                     /locus_tag="ACHAYA_002744"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..>4840)
                     /locus_tag="ACHAYA_002745"
     CDS             complement(4240..>4840)
                     /locus_tag="ACHAYA_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606479.1"
                     /translation="WKAVFLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNL
                     IIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALL
                     LCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAIL
                     VLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1476 a         1008 c          778 g         1578 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa
//
LOCUS       DAYYCW010000147         4798 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000147 DAYYCW010000000
VERSION     DAYYCW010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.149"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACHAYA_002746"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACHAYA_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM8606480.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="ACHAYA_002747"
     CDS             complement(2066..3064)
                     /locus_tag="ACHAYA_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM8606481.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="ACHAYA_002748"
     CDS             complement(3290..3943)
                     /locus_tag="ACHAYA_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM8606482.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACHAYA_002749"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACHAYA_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM8606483.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1407 a         1007 c          744 g         1640 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt atacataatc aacttttaaa tagctacctt tttttcataa ttttttta
//
LOCUS       DAYYCW010000148         4746 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000148 DAYYCW010000000
VERSION     DAYYCW010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.150"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACHAYA_002750"
     CDS             complement(<1..300)
                     /locus_tag="ACHAYA_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFM8606484.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACHAYA_002751"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACHAYA_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACHAYA_002752"
     CDS             complement(1014..1304)
                     /locus_tag="ACHAYA_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606485.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACHAYA_002753"
     CDS             1706..1942
                     /locus_tag="ACHAYA_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606486.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACHAYA_002754"
     CDS             1949..3901
                     /locus_tag="ACHAYA_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8606487.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACHAYA_002755"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACHAYA_002755"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8606488.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1626 a          826 c          772 g         1522 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagt
//
LOCUS       DAYYCW010000149         4698 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000149 DAYYCW010000000
VERSION     DAYYCW010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.151"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACHAYA_002756"
     CDS             complement(543..2048)
                     /locus_tag="ACHAYA_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8606489.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACHAYA_002757"
     CDS             complement(2045..2731)
                     /locus_tag="ACHAYA_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8606490.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHAYA_002758"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHAYA_002758"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM8606491.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1327 a          964 c          777 g         1630 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa agggatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attttggctc
     4441 tataatttat gggactgatg aatcagaatt gattcttcag ccccattttt attttaggta
     4501 ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca actgagagga
     4561 cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag aaagatttga
     4621 tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc gtcgatgctg
     4681 ttttctcacc tgctcccc
//
LOCUS       DAYYCW010000150         4587 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000150 DAYYCW010000000
VERSION     DAYYCW010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.153"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACHAYA_002759"
     CDS             186..2855
                     /locus_tag="ACHAYA_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM8606492.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3560)
                     /locus_tag="ACHAYA_002760"
     CDS             complement(2946..3560)
                     /locus_tag="ACHAYA_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8606493.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="ACHAYA_002761"
     CDS             3680..4219
                     /locus_tag="ACHAYA_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606494.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGEMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="ACHAYA_002762"
     CDS             4216..4554
                     /locus_tag="ACHAYA_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFM8606495.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1420 a          790 c          859 g         1518 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggagaaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgac
//
LOCUS       DAYYCW010000151         4514 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000151 DAYYCW010000000
VERSION     DAYYCW010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.154"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..1261)
                     /locus_tag="ACHAYA_002763"
     CDS             complement(29..1261)
                     /locus_tag="ACHAYA_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM8606496.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="ACHAYA_002764"
     CDS             1516..2085
                     /locus_tag="ACHAYA_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8606497.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="ACHAYA_002765"
     CDS             complement(2263..2703)
                     /locus_tag="ACHAYA_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM8606498.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="ACHAYA_002766"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="ACHAYA_002766"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM8606499.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..>4514
                     /locus_tag="ACHAYA_002767"
     CDS             3657..>4514
                     /locus_tag="ACHAYA_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM8606500.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKK"
BASE COUNT         1351 a          844 c          898 g         1421 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaa
//
LOCUS       DAYYCW010000152         4464 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000152 DAYYCW010000000
VERSION     DAYYCW010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.155"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..1018
                     /locus_tag="ACHAYA_002768"
     CDS             137..1018
                     /locus_tag="ACHAYA_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8606501.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1031..2641
                     /locus_tag="ACHAYA_002769"
     CDS             1031..2641
                     /locus_tag="ACHAYA_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8606502.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2718..3377)
                     /locus_tag="ACHAYA_002770"
     CDS             complement(2718..3377)
                     /locus_tag="ACHAYA_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8606503.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3660..4325)
                     /locus_tag="ACHAYA_002771"
     CDS             complement(3660..4325)
                     /locus_tag="ACHAYA_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM8606504.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         1215 a          860 c          876 g         1513 t
ORIGIN      
        1 tcattgacag aaatttataa aaagaatatt atgtactaaa ataataaaaa aagttttata
       61 aaaaggtata aaaccttttt attaaactat ttaatcttgt ttagtatata tttttagaaa
      121 aaggagtggg gtttttatgg cagaaatcgt caaagtcaca catttgaaaa agagctttgg
      181 tcgtttcgaa gctttaaaag atgtctcttt caccgtacat tcaggtgaag tcgttggatt
      241 tatcggacca aatggttctg gaaaatccac gacgattcgg acacttttag ggattataaa
      301 aaaagacggc ggaacggcaa cgatttttgg gcaagatgta tgggaagata gtttatctat
      361 ccatgaacgt ctttcttatg ttccaggcga tatctctttg tggggaaatt tgactggagg
      421 agcgatcatc gatctcttca tgaagcttca tggaaaaggg agtgctcaaa aaaaagatga
      481 actgattcaa cgtttctctc ttgatcctaa gaaaaaagca aaaaactatt caaaaggaaa
      541 tcgccaaaaa gtgggcttga ttgctgcttt ggctgttgat tcggatctct atttatttga
      601 tgagcctact tctggtttag atcctttgat ggaaacgatt ttccaagaag aagttgaaaa
      661 attgaaagaa cagggcaaat ccatcctact ttcttctcat attctcagtg aagtcgagcg
      721 actagcggat aaagtcgtga tcatacgcga aggaacactc gatgaagaag gaacactcga
      781 tgaattgcgt catttgactc gttcgaccat cactatagaa acagaagcag agaatagtca
      841 cttagcacaa ttgaacggtg tccatgactt tgtgcaaaaa ggtcacctcg ctcaatttgc
      901 tgctgataac gaagctttcg atgtgatttt agctgaagcg tccaaattag gtgtgaaaaa
      961 atttgaatct attccaccga ctcttgagga tttattcatg agacattatg aaggatgagg
     1021 aggaagcgaa atgactgata aattttcacg ctggtccacc cttttttctc aaaaccttag
     1081 ccgtgactgg aaaaagatcc tgatatggct agtcggtgtc actgcttttt ctggtggttt
     1141 tgtccctgcg tttgaagaat tgacttccgg acaaggatta gctggattat acgaaacctt
     1201 gcaaaacccg gcaatgattg caatggtcgg accaactcca gcttcgaatg ctgctgagta
     1261 tactttagga gcgatgtatg caaatgaaat gttgcttttt tgcagtttgt ttgctgctgt
     1321 gatctctgtt ttacacgtgg tgagccaaac gagaaaaaaa gaagaacttg gtctgattga
     1381 acttattcgt tcctttcgag tcggacgtca agcaaactct ttagctgtca tcgctgaaag
     1441 tattctgatc aatttgcttt tagcaatatc gatcagctgt acgatgattt ttttccaagc
     1501 ggattcgatc actgcagaag gtgcttggct ttttgggcta tctgtaggta gtatggggat
     1561 tttggggagt gccgttgcac tgtttgctgc acaactgatg ccaagttcag ccagttcgaa
     1621 tggtctgtct ttagcgtttt taggtggact ttaccttcta cgagcaataa cagatgtagc
     1681 tgattcctcc ctttcgatgt ggaatccttt aggctggtct tatttgacct atccatttac
     1741 aactaacaac tggattcctt tgctttattt gtttttgttc agtctaatct tgataatcag
     1801 ttcatttgtt ttagaggaac atcgagattt gaacaccggc tatatccccg aacgtgaagg
     1861 aaaaccgtat gctaaacgtt ctcttttatc cttgcgtggt ttattattct atctaaataa
     1921 agggatgaca atcagttggc ttatcacttt tggtttgatg ggggcggcat acggctctat
     1981 ctacgggaat atgcaacagt ttttagaaag taacgactta atgaaacaga tgtttatcca
     2041 atctggcgtc tcaattgaaa aaaactttac aagcacgatc atggtagtct tgatttgtct
     2101 agctgctatt ttaccaattg ctagcatcaa tcgtttattt acagaagaaa cccacggacg
     2161 tatgaatcag ctgttcgcaa caaaaatttc taggaaccaa ctttattgga ccaatatgtt
     2221 actagctatt ctatttgcta tattggcaat cctgattagt tctggtgcac tagggctttc
     2281 tgctttggct tcgatgaagg atgcgaaaga catggatcta ttgatgtttc ttaaagccgg
     2341 gttcaatttc ctgcctgccg ttttagtgtt tatcggaatt agtggctttc tattcggatg
     2401 gcttcctcga atcggtaaac ttgcttatat ctacttagct tactcctttg cactcaatta
     2461 ctttggcgga attttggatt taccagaatg gttttccaaa acagctgtcc ctagctgggt
     2521 tccacggatg ccaaacgatt cttttgatct ttccgtgttc cttacgttat catgtatcag
     2581 ccttgcactg ttcgtccttg gttctattgg ttataggcga agagacttac tagaaggata
     2641 ataaaaaagc atgtacaatt tcatgctagc ctccaaatct gggtcaaaaa tccaacattt
     2701 ggaggtcatt tttttattta caagtcttct aactgctctt tatcaatgat ccagacttct
     2761 cgattctttc tttttacata accattctcc tctaataatt taaattttct agataaagtt
     2821 tcgggggtcg taccgataaa tgcagcaagt tctttcattt tcattggcag gaaaaagtga
     2881 tccttccctg tcgctttagc taagtcaagc agatagagag cgactcgttc ttctactttt
     2941 tccatcatca aaaaagttgt ctgctgctgt acttttgtta atttctcaac gttgattgct
     3001 aaaagtttca gactaagggc gggataatgc agtaagagtt ccgagaaatc tttttgtctc
     3061 aatatgcaaa tttccgtttt ttctagtgct tcgccaaata aagactcatt tggaacgcct
     3121 agcaactggc tctctccctc atatccgccc ggctctatta ctcgtaaaag ctgttccttt
     3181 ccgctagcag ataattgata cactttcata ctgccggagg ccacgatcac taattgaggt
     3241 tctccattcg gcgcaagaat ctgttctcct ttttccacat agtgatgttt gacaagtgcg
     3301 tgtacttttt cttgatcttg ctgtactaag tgattgaaca aaggaacaaa tgaaacacat
     3361 aaatgatcat gactcataat ctctcctcct tctaaacagc tatttctaat aaagaaacat
     3421 aacttccctt attccaactg ataattttct tttatcatag cataaacgtc aagcgaacca
     3481 gataccagca aatagagtgg atttcttttt gtcccttgct ttattttcct tcttttgaga
     3541 ccttaagtaa ttgattccta tcaatttttt aaatatttac tttttgaata atcaaaaaca
     3601 aaccttcact cgcaagccga gagtaaggtt tgtttttcgc attgcttatt tcgtgttgtc
     3661 tattgaaatt tttccgcacc tagcttttga tatagtggtg ctagttcttg taaggtttca
     3721 gcggttattt gctcaacgtg ttcgatttct tgcggataga agaatagttt acccatttga
     3781 aaggttcggt ctctcatcgc ttttttcaac tcttcttgtt tctttgtatc ggacaaatag
     3841 tcagacaaag ctaagtggtc gtcaagttca tgttctggtt gcggccatat gtaataatcc
     3901 ttgatatcga ctgttttgct ccactctttc agtatcgcaa gcaactggtt atacgctgca
     3961 atgctaccat atcgttcttc gcttttataa tgttggtaca tcaaatagat ttggtattgt
     4021 ttttgattca acaaaactgc tagacaggca ttctcgtctt gacgagattc gcttcggtac
     4081 gcgctccaaa aatgactgcg taaattccag ccattcgtcc agctttcgat tttaggtttg
     4141 ctcatgccgt acatatcagg caaaagtgca gctgtctgga gctgaatctc tttccatttt
     4201 tgccagtgtt ctttatattc tttcttgatt tgttcaatct tatctgttgg atggctctct
     4261 ttgagctgtt tgaatgcgta gattggtcga tcaaacaact gataatctga ttttccaagt
     4321 tccattgttt cgcctctttc cgttatcttt tgtattctac ctgaattttg cgatatggac
     4381 aaatagaggt tgtgaaaaag ctgttctgca tcaagtcata agaacagcca aacaaaaaac
     4441 tgcgatgagc atcaccgcag tttt
//
LOCUS       DAYYCW010000153         4385 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000153 DAYYCW010000000
VERSION     DAYYCW010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.157"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            262..990
                     /locus_tag="ACHAYA_002772"
     CDS             262..990
                     /locus_tag="ACHAYA_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8606505.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1135..3162
                     /locus_tag="ACHAYA_002773"
     CDS             1135..3162
                     /locus_tag="ACHAYA_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM8606506.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            3270..4367
                     /locus_tag="ACHAYA_002774"
     CDS             3270..4367
                     /locus_tag="ACHAYA_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8606507.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1539 a          713 c          912 g         1221 t
ORIGIN      
        1 gtcacatttt gatagatagg agggggactt acgaattatt ttaaaggcta tgacagctgt
       61 atttctagat tgtgtttgca tcaagttgca ataaaaacta gaaatcaaat cgggctgata
      121 atcatttata actaatcgat acataggacc aaatataggt ctatgttctt ttatttcata
      181 agtatcgtaa aacagaattt tgctatggat taatccaatc gactgatatg tttagtaaat
      241 aaagaaagag gaatgtcaat gatgaaaaaa gaaatcgtat taacagcagc aatgatgtta
      301 ttcagcatgg tagcaagtac gacttttgtt tctgcaactg aagtataccc aaaggaatat
      361 aacacagaag gaacgattac atttgaagca ggagatgagg gtgtaactcc tcctgttgat
      421 ccagagaatc ctgatcccaa taaacctgtc gatccatcag atcctccaag ccctggaaca
      481 ggaggggcat tatcgattga ttatggatca aaattcaaat ttgggactca aaaaatttct
      541 actgcggata aaacgtatta tgcagctgct gatgtgatga acgatgggtc tagaaaaccg
      601 acatatgtcc aagtgacaga taggagaagt accttatctg ggtggaaatt aagtgtctct
      661 caaccagaac agtttaaaac tgccagtggg gatgaactag ttggagcaca gctgaaattt
      721 acgaaagggc aggctgtttc tttggttgat ccaacatata cgccgcaaac ggtgaattct
      781 gaattaacgt tgacaccggg aggaaacaat acgttagcaa ttaatgctaa atcaggaaca
      841 ggtgtgggga cttgggtcta tcgatttggt gcaaatgaaa atgaaaatca agatgcggta
      901 cagctttcgg tacccggtaa atctgtcaag ttagcgcaac aatattccac taaattagtt
      961 tggacattag aagatacacc taacaactag tttactgaaa agaactgtta aaaataaata
     1021 cgtagtcata ttgtcttgcg aggtaatcga gcaaaaattg tcaaaggcta ggctaaattt
     1081 gtttggatac gaatagttca tctaggaatt taacgatcag gaggaaaaga tatattgaaa
     1141 aagaggagga tcgtgctcat tttaagcagt ctcttagtga ctaatatctt tccgatcata
     1201 gggtactctt tggaattgaa tcagttggag catggaaata gaagagacga agaaagcaaa
     1261 ctcacttcga cattcgacag tttaatagaa tccaaccaga cacaagaaag cagtgaggat
     1321 ttaagtattt cgagtagtgg agagaccact gacaacgtag aagctgaggg ttcggacaaa
     1381 caaggactag aagatgcacc aatttctgcg gttgagctgc ctgacgatcc caatattatt
     1441 tctatcgata aaatttttca aacacctatc ggtactagca cgtccatatt ggaaaatggg
     1501 aagttactgc agttgaaccc agcagaaagg tctcaaagag gagctatttg gtcaaataag
     1561 aagattagtt tactctcgga ttttacattt aaatcttatt tatacttagg aaatcaacga
     1621 gatagagcgg gagatggcat gacctttaca ttgactggcg atcctagaat ggctagtaat
     1681 cccagtcaag ttattggatc gccaggtatg ggaattggct catattctac tcgttctggc
     1741 caaccttaca ttgcgaatgc gctctcgatt gagtttgata cgtataaaaa tagtggttct
     1801 tctgatcgaa tggatcgaga gataactgct gataatagag ggcatggaca tgtcgctttt
     1861 gtcacgccta aaccaaataa taataattat tctggagagc atactggcgt gacggtagct
     1921 cctacatttc tttctaatgg cacatggcgc ttgttaataa ttaaatggga cgcggagcaa
     1981 agaagtctta cttatgattt ggaaggtgtc ggtcgctctt ctcggattat acctgatctc
     2041 attaggcagt ttggttctac agaagtaaat tgggggttta cttcttcaac aggtactttt
     2101 tttcaagaaa atgctgtcgc tgtcacgcga cttccatcga atgtcacatc aacagctgat
     2161 gtaaaagtca acgatagtga ttatggggcc atcgctgaag ctggaggaaa cgataagata
     2221 actatcagaa atacattgaa ggtcgagggg gattttcttg aatataagaa aatgcagact
     2281 agtatcgacc tcccacaaga attaactatt cccgagcata tcttgatcaa tggaaatcaa
     2341 attgatgcca aagatatctt gataaccgga aatcgagtca cgctaaatct gacagattat
     2401 tttattgctg gagatacgtt aataatcgaa ttggagacaa ctttgcagga agtaattcct
     2461 gaaaaagtac tcactatgca ttttgaatac cttgaaaacg ggaatttgat tgagcaatcg
     2521 aatgaaataa acctaaagat cgcaaagctg aaggagatag atatccatgt ttattataaa
     2581 gataaagaca gtcagattga tattgctgaa atgaaaacaa tcactgggaa aataggagag
     2641 aagtataaag aaactcctat tgatattgaa gggtatgttt ttgactcgga ttcagcgaat
     2701 acagaaggca tcttctccga aaatacagag gatatctatt tttattacag aaaaggcaaa
     2761 ctttatctct caactgctcc tcaatatgtc aattttggaa aacataaaat atccagtaga
     2821 ccattaagtg aattcggaca gttttcaggt gggttgaaaa ttatagatga acgggctttt
     2881 ggaagttgga aattacaatt gaaacaagag aaagttctta caaatggaga ttttgagctt
     2941 ccagaagctc tttcctttgt tacctctgaa aatacaacgg ttatagggac atccgctgtt
     3001 acgattttcg aaagtgatca aaagggtgaa agcgatttgt cctctctttt agattctaca
     3061 gctcatcgtg gaatcagaat aaatattcct gtagaaaacc aaagaatcgg aacctttgag
     3121 gggaaactat cttggatact tgcagatgtc ccaggaaatt aacaggaaga gaagaatata
     3181 acaaaattat tgagtttcaa gaagggcaca ggtaccatag gctcttgtgt cccacagaga
     3241 caagattttg ttatagtctt ctcttacata tgattaaagg agcgattttc ttgagaccaa
     3301 aacaacaatt atttataaca ctcctagtta ttttaggggc actttttaac aatatatcag
     3361 tgtcagctag tgcatttaat ttttcagtga cgccgataac ttcagagaat caaatcgaca
     3421 agagaaaaac ttacttcgat cttcaacttg atccggatca ggaagtagaa gtaaaagcgg
     3481 aattgagaaa tgatacagaa aaagaagtga aaatcgatat ttctgtgaat agcgcaacca
     3541 ccaattcaaa tgtgatggtt gaatacggaa aaaacgagat tgaaaaagac atgagcctga
     3601 tctttgatct ggtagattat gtgagctatc cacagatagt gacgctaaaa cctaaaagtg
     3661 tccaaaccgt tgcttttcat gtcaggatgc cgaatgagcg atttgatggt gtattggctg
     3721 gtgggatcac attccaagaa atccagacgg aaaaggacca aacagaaact aaggatcaaa
     3781 gcttatcgat tgagaatgaa tacacctata ttattgcttt gttgatgcaa cagacactga
     3841 acgaagttgc tccgaacctt actcttcatg aagttaaacc agatcaaatc aatgcaagaa
     3901 atgtcatctt agcaaacgtt caaaatgatc aaaaaaccta tatcaatcaa gtagttattg
     3961 aaacaaaaat tacaaaaaaa ggacattctg aagttttata tcaagaggaa aaagaaggat
     4021 tgcagattgc gccgaataca aatttttctt ttccaactgc tttaaatggt caaccgctaa
     4081 ccccaggtga atatcacctt acaatgactg ttttaggaaa tgaaaatgaa aatggaaaat
     4141 tttcgagaaa aaaaggaaat actacgatca actataccaa tcaatgggta tttgaaaaag
     4201 attttacaat agacggaaaa gtagccaagg aattgaacac gacggatgtt acgattaaaa
     4261 aagacaataa ttggatttat atcttagtag cttcactttt cttattgttt attttattat
     4321 gcatactttt gtttgttttg agaaaaaaag aagagaaaga aaagtaaggt taagataaca
     4381 aatag
//
LOCUS       DAYYCW010000154         4366 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000154 DAYYCW010000000
VERSION     DAYYCW010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.158"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            167..868
                     /locus_tag="ACHAYA_002775"
     CDS             167..868
                     /locus_tag="ACHAYA_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8606508.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1440..2456)
                     /gene="tsaD"
                     /locus_tag="ACHAYA_002776"
     CDS             complement(1440..2456)
                     /gene="tsaD"
                     /locus_tag="ACHAYA_002776"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM8606509.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2453..2917)
                     /gene="rimI"
                     /locus_tag="ACHAYA_002777"
     CDS             complement(2453..2917)
                     /gene="rimI"
                     /locus_tag="ACHAYA_002777"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8606510.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2924..3466)
                     /gene="rimI"
                     /locus_tag="ACHAYA_002778"
     CDS             complement(2924..3466)
                     /gene="rimI"
                     /locus_tag="ACHAYA_002778"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8606511.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3450..4172)
                     /gene="tsaB"
                     /locus_tag="ACHAYA_002779"
     CDS             complement(3450..4172)
                     /gene="tsaB"
                     /locus_tag="ACHAYA_002779"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM8606512.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1298 a          925 c          744 g         1399 t
ORIGIN      
        1 tcgcttcaaa acaaattatt caataccatt gtatattttt tacataataa caaattatat
       61 ttgtaaagta ttaaaacttg tttctaatgt tggtatgtta aaactttaca aattctttaa
      121 aaaaagtgac aataaatata gtgaacaata aggagaggac tttttaatgc gtgttaataa
      181 aatcgatttt cacttttcaa aactcagata tcaacctgat tttgaacatt tctcggatga
      241 agagttccaa cagatcaagg aacatactgt tcttcgttct tataaaaaag gacagatcct
      301 tttcgacgat ggtgatccca gacaacggtt gtatatcttg accaaaggac tcgttcgttt
      361 agaacgttat gatgaaagtg caacttatta ctactatgac tatgtgaata caaataacct
      421 ttttcctatg tcaggaatgt ttggagaaga gacctacact tatacagctc aagcgatgac
      481 tgatattgaa acattctacc ttcctgtttc ctactatgaa aaattagcac gaaaaaatcc
      541 cgaacagttg atctatatga tacaaaaaat ttctaaagta gttgaaatac atgaattgcg
      601 tatccaaatc ggattgacct ctagcgcctt tgaccgggta aaacaaaatt tattcatttt
      661 gaaagaagaa ttgggcgtac caacaaaaga tggtcatatc accattcctt atccgatcac
      721 cttaaaagaa ttagcagtga atagcggcac gacacgtgaa actgctggac aagtaatcaa
      781 acaactacgt gattccggac tgttagatta tgacaaaaag cgttttactt tctgtaagac
      841 agctactact ccagctatga gagaataacg cgaacaaaaa taagcagtga ccctgatatt
      901 tatcagggtc actgcttatt ttcctcccta aaacttctga aagatccggc agttttagat
      961 cactgggcaa gactcaaaga ttttctatgt gtgtttcagt tatgtaatat tcactgtttg
     1021 caaaaaatac tgatagatta actagaggaa gcgtcttgtc ctcatccgac ctagattatt
     1081 cgatattcct cgacaataaa cgattaatca gtgagggcaa tcagaggaac ggataatgat
     1141 gaataagctg taaaaaatag ggctttgaca aaagcgtgtc acagccctta tttcgattaa
     1201 acggtgtgta cctgtcaact cttcggtcac aagtcataat gttcataaac atgaggagct
     1261 gttttttgaa attgttcctt gtgaatcaga gttaaacgac aggcttacga actcttgatt
     1321 aaacggcgtt caaaagcttg catacgcggt aataagaaca gccaggtcaa aaacatgaga
     1381 agccgttttt cctgggctgt tcttattact tgatgcaggg cagatttttc acaacctctt
     1441 tattcagata aaaccaagct tggatcagca ttcaactgat agctagcaaa atttcttttt
     1501 tgcatttcca catgtgctgc tgctccaatc attgcagcat tatctccaca aagtcgaaga
     1561 gggggaatca ctagttctac ctcagggagt ttagttgcca aagcatcctg caatccttca
     1621 cgcaaccctt gattggccgc tacacctcca gctacaatca gttgcttgac tggataattt
     1681 tgacaagctc gaagtgtttt agtaatcaat acatcgatca cacttgcttg aaagctagct
     1741 gctaaatcat ttttgtctag atcttctcca cgctgttgtg cattatgcac caaattgata
     1801 aatgcacttt ttaatccgct aaaactgaaa tcataattat cttcatggat catcgctcgt
     1861 ggaaaatggt aattatcttt tccctgatga gctagctgat cgatttcttt tccgcttggg
     1921 taagaaagac ctaatactct tccaactttg tcataagctt ctcctgctgc gtcatctctt
     1981 gtctccccaa tgatttcata tgatccatct tcttgcatat agactaattc tgtatgtcct
     2041 ccgctaacaa gaagtgccat caatggaaac tggaatggtt taaccagacg ggctgcatag
     2101 atatgccctg ccatatggtt tacaggaata agcggcaatt ggtgtgccca agcaaaagcc
     2161 ttagctgcag aaataccaat cagcaacgag ccgacaagtc ctggtccata agtgacagcg
     2221 actgccgata aatcttctgc cgacacacca gcttctacca gtgcatcttc caagcataag
     2281 gtgatttgtt ctacgtgatg gcggctagct acttcaggaa ctacgccacc aaaacgttta
     2341 tgactgttta tttgagaagc aacgatgtta gataagatct ctgttccatt tctaacgacc
     2401 gctacactcg tttcatcaca acttgattca atcgccagaa tcaattcttc attcatttca
     2461 tcgtctccgt ttttaacttt ctgctcataa tgatcgcatc ttccgtcgga ttgcgataat
     2521 atgatttccg tttacctaat atacgaaact tttctgattc ataaagtttt cttgctgctt
     2581 cattagatag acgtacttct aaaaaaacat gcagcgaatc ttttatttgt tccgtaatca
     2641 gtaattgtaa caaatgccgt gcatgccctt ttccttgttc tgaaactgca acagcaatat
     2701 ttgtgatctc gacttcatct attactttcg agtatccaat aaagccacag atctggttat
     2761 cttcaactaa aagcagataa tcagagtgag gctgttggat gtctgccaaa aactgcattt
     2821 tcgtccaagg tgaaccatgt ggataagcat tttcgctaat catccatacg cattctgcca
     2881 agtacgcttc atcaaaatct ttttttctgc aaatcaacta atctcactca ttttctaaaa
     2941 gttttatcat atccaacgca tcttcttttg tttcagtgta atatcttttt ttgatcgttg
     3001 ttgcttgaaa acctaacttc cgataaagtc tttgtgcatc ttgattactc atacgcactt
     3061 ctaatgacat cgtctcacag cgattcatta tagcaaagtt ctcgatttcg tcaataagca
     3121 gcgaaccgat tcgtttacgc tgatatgccg gtcgaacggc aatattcgtg atatggcaat
     3181 ctaaaccgaa cgctcttgaa ccgataaaac caatcattct tccattatca aagataccta
     3241 gatatagatg aggaagcggt gacttcaatt ctgatataaa ggcacttttt gtccatggca
     3301 attcgccatt atagacttct cgttcgacat ccagcagctc tttgatatct tctgtagtca
     3361 attctcttag tacgtaacga cgatcgattt gttcgattgt tttttctgta taaatcattt
     3421 tatcttgaaa aaagcttctg gcatacgctc taaatttttt caacataact ttcgtctcca
     3481 ggtgtgtgcg tctccaacca tttttcttct gcttctaccc gttttaaata gcgaggaaga
     3541 aaagcttgtt tctctgcaac tggttcagct aaagcaccta attgagccac agttacacca
     3601 cttggaatat cccatgctgg aactgtatta atcatcgcac taggcaatat ttcctttatt
     3661 tcatagaaga atttgtgaca atcactcccg acgaaataaa tagcttcatt cagcttttgt
     3721 agttgtgtga acaaatcaga agcagaaata tgtacatctt caagtacaat ttctaatcta
     3781 ttttctttcc agcggtatgc cccagcgtat acattttttc ttcgagcatc aaacatgggc
     3841 acaataattt tatccacacc gatgcaatta gccgcgatgg ttttcagact agatactcct
     3901 actagctctt tatctaatgt ataagcaatc gtttttgctg tcgttacacc tatacgaagc
     3961 cctgtataag aaccgggacc atcagaaacc gcgattcggt caatgtcttt tggacttatt
     4021 tgaagatctg caaacaattg gtcgattgca ggcatcagtg ttacactgtg gttgcgtttg
     4081 atatttgttt gtatttgtcc aagaatttgt tcgtcttcta ccacaccgat cgccaaagtt
     4141 tggtttgcag tatctattcc taatgtaatc atatatttga tcctttctgc tatgacattc
     4201 tcgccttcca ttgtagcata cttttttatt ctgaggatga aaataccaaa ggcaaaacta
     4261 gtacaatgat acatgattat ctgctggcgg gcaaattttg ccccttcctg aaacacaaaa
     4321 aagctgcggt atcacttcat ttccccacag cttttccatc ttttat
//
LOCUS       DAYYCW010000155         4365 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000155 DAYYCW010000000
VERSION     DAYYCW010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.159"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..1075
                     /locus_tag="ACHAYA_002780"
     CDS             152..1075
                     /locus_tag="ACHAYA_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM8606513.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1081..2334
                     /locus_tag="ACHAYA_002781"
     CDS             1081..2334
                     /locus_tag="ACHAYA_002781"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM8606514.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2468..3814
                     /gene="gor"
                     /locus_tag="ACHAYA_002782"
     CDS             2468..3814
                     /gene="gor"
                     /locus_tag="ACHAYA_002782"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM8606515.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         1504 a          690 c          939 g         1232 t
ORIGIN      
        1 tcacaatctc tttttatatt tggacagaat atatcgaact ttattttgat tttttgtgaa
       61 ataattttca aaaatccgac tttaaataga agaagtattg gaaagttttt agagctatgc
      121 tactattatg aagttgtagg aggagaaaat tatgatcgaa ctcataacca cagtagaaac
      181 tattgaacaa gcagaacagt tgcttgctgc aggtgcagat acactatatt tcggtgaaga
      241 gcaatttgga ttgcgtctgc ctgcttcatt tacacgagaa gaacagcgac aattggtaga
      301 attagcacac aagtatggta aaaaagcgaa tattgctgta aatgggatca ttcatcctga
      361 aaaaatgaaa aatgtacctg aatatttggc ttttttaaaa gaaataaacg tagatgtgat
      421 cacagttggt gatacaggta ttatttatac gatgaaaaaa aatcctgaat tgcgtatacc
      481 gtatatttat gatgctgaaa cattagtaac gagcgcacgt caaatcaatt tctgggcgaa
      541 aaaaggcgca gttggagcag tacttgctcg tgaagttcct tttgaagaga tgaaggcgat
      601 ggaagaaaaa ctcgatattc ctgtagaaac ccttgtgtat ggtgctacat gcatccatca
      661 atccaaacgt ccactgctac agaattacta taactatacg aaacaagacg aacaaaaaga
      721 tcgtgaaaga ggtcttttta tctcggaacc taaaaaagaa gagacacatt attctattta
      781 tgaagatagt catggcacac atatttttgc cagcaatgat ctgaatctct cgaatgaatt
      841 gaatgaactt tatcagcatc attactgtac atggaaattg gacggattat ttactccagg
      901 cgaaaaattc gtaaacatca ctcgactatt cgccgaagca aaaaaacaaa ttttagaagg
      961 tagctgggat caagaaaaag cggaaaaaat gactagcgaa atcagtaaat tgcatcctga
     1021 aaaccgtgaa ttagatacag gattctttta tattgatcct tctgctatca aataaggagt
     1081 atgatgaaag aaatgacagt aaaaagaaaa ttgaaacgac cagaaatcct agcgccagca
     1141 ggaacactgg aaaagttaaa aaccgcgatc cattatggag cagatgcagt atatattggc
     1201 gggaatgcat atggtttaag aagccgtgca ggtaatttta cttatgaaga aatggctgaa
     1261 ggagttgctt ttgcaaaaga acatgatgcg aaggtatacg ttgctgctaa tatggtcacc
     1321 catgaaggaa atgaagatgg agcaggggaa tttttccgca gtttgcgtga tgtcggtatt
     1381 tctgcagtga ttgtttctga tccagctttg atcgaaattt gtgcgacaga agccccaggt
     1441 ctgccgatcc atttatccac tcaagcttca gcaactaatt ttgagacatt ggaattctgg
     1501 aaagaagaag ggttggaacg tgtcgtttta gctcgtgaag tttcaatgga ggaagtggca
     1561 gagattcgta aaaatacgga taccgaaatc gaagcattta tccatggtgc catgtgtatc
     1621 tcttattctg gtcgatgcac attgtccaac catatgtcga tgcgggatgc aaaccgtggc
     1681 ggatgttctc aatcttgtcg ctggaagtat ggattgttcg atatgccttt tggtcaagaa
     1741 cgaaacagtt taactgatac aggcgaagtg gaagaagaat tctcgatgag tgcagtggat
     1801 atgtcaatga tcgaacatat tccagaattg attgaaaacg gcgtagacag cttcaagatc
     1861 gaaggaagaa tgaaatcgat ccattatgta tctacggtgt caaatgtcta taaagcggct
     1921 gtagacagtt atatggaaga tcctgaaaac tatgtctgca agcaagaatg gatcgacgag
     1981 ttatggaaag tagcacaacg agaattagct acaggttttt actacaatac accaactgaa
     2041 aacgaacaat tattcggtga acgtcgtaaa atcccagtat ataaatttgt cggtgaagta
     2101 ttatcttatg atgaggcaac acagattgct acgatacgtc agagaaactt gttttctgta
     2161 ggagatgaaa tcgaatttta tggtccagga ttcagtcatt tcagtcaaac cgttgaagtt
     2221 atgcataatg aagaaggcga atccattgac cgagcaccaa atccaatgat gattttaact
     2281 atgccagtcg cacagccggt gaaacctgga gatatgatcc gaaaacgtaa ataaggaaaa
     2341 agctgatata tccctgtttt aagcatcttc gcatacaaaa ttcaagaaaa gtgttacact
     2401 tgttttctgt agaaaaagga tcgttaaata aaggttttga ttgatctctt caataaggag
     2461 gaacaaaatg caaatatatg attacatcgt aattggtggc ggctctggcg gtattgcttc
     2521 agcaaacaga gcagggatgc atggcgcaaa agttttgctg attgaagcga atgaattggg
     2581 cggaacatgt gtcaacgtcg gttgtgtacc taaaaaagtt atgtggcaag caagtacgat
     2641 tttggaaaca attgaacgag atgcctcaag ttacggtatc caagcagatt taaaaaatgt
     2701 tgatttcgcc ggtctagtag aaagacgaga aaaatatatt gattttctac acggtgctta
     2761 tcaaagagga ttagatagca atcatgtcga ggctataaaa ggttacgcta cttttatcga
     2821 tagtcaaacg atcgaagtaa acggtgaaac ctatcgtgct cctaaaatct tgatcgctac
     2881 tggaggccgt gcaagtaaaa tgaccattcc tggtggagaa tacgcaattg attcaaatgg
     2941 attttttgaa ttaacagagt tacctaagag agcaatcgtt ttaggtgctg gatacattgc
     3001 ggctgaactt tcaggtgtat ttcgtggttt aggtagtgaa gtcatgtggg catatcgaaa
     3061 agaacgacca cttagaacgt ttgataaaat gctttcggac aatttgatcc aaatgtacca
     3121 agaagctgga ataaaaactt acgcacatca tattgctaaa gaaattacta aaaacaatga
     3181 cgaatatacg gtgctatttg aaaatggtga aaccttaact ggtgattgtg tactttttgc
     3241 cggtggacgt gtaccgaata ccgaaaaatt aggtctggaa aatacaaatg tggaattgga
     3301 caaaaaaggg tttatcaaag tagataaata ccaaaataca acggatgaac atatttttgc
     3361 tgttggtgat gtgatcggta aattagatct gactcctgtt gcaatcgcag ctggtagacg
     3421 tttatcagaa cgtttattta atggaaagaa agattcttat cttgactata aacttgttcc
     3481 gacagtcgtc tttacgcatc caccaattgc aacaattggt ttgacagaag aagaagcatt
     3541 ggaaaaatat ggcgaaaatg aattgaaagt ttatcgttca cgctttaccc caatgtactt
     3601 tgcattaaat gattatcgcc aaaaatgcga aatgaaattg atctgtgtcg gaaaagaaga
     3661 aagaatcgtc ggcttacatg ctattggtat tggtgtcgat gagatgctgc aaggttttgc
     3721 cgtggctatc aagatgggtg caactaaaga agactttgac aatacagtag ccatccatcc
     3781 aacaggagcg gaagaattcg taacgatgcg ataatttaat attaacagtt ccaacagctt
     3841 tttattgaac aaaccatgta aaaccagaaa atcagtatac tgattttctg gttttttgtt
     3901 ttttagttat ttatgtaaga ttgcttcgat aattctcaaa acgaaataag tagggaagtt
     3961 ggagattttg attatcttcg taactgacga agagtaaatg aatccatttg gtatgcactc
     4021 ttaagttcaa aatattggta tcgatgttta aaagaaatag aaatgtatcg aagaacagat
     4081 gtttaaaaaa gtgtgacttt gtataaataa attagaagaa gcaaacgagg atgttaaaag
     4141 aataccatat agttaccaaa ccaagaaagt tctcgtcaag ttcgctggta cgaatcaaat
     4201 caagtgaaaa aagggactgt gaaaaaaatt gtgtcatagt tagtcacttt ttccgaacaa
     4261 atagtgttca atcagctgtc cttcggtatt agctcaaaaa acggcaaaat atgaggaact
     4321 atttttccat ttgtttctct aatactcagg acaaaacact gattt
//
LOCUS       DAYYCW010000156         4344 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000156 DAYYCW010000000
VERSION     DAYYCW010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.160"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(97..582)
                     /locus_tag="ACHAYA_002783"
     CDS             complement(97..582)
                     /locus_tag="ACHAYA_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFM8606516.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(584..1255)
                     /locus_tag="ACHAYA_002784"
     CDS             complement(584..1255)
                     /locus_tag="ACHAYA_002784"
                     /inference="COORDINATES: protein motif:HMM:NF037286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606517.1"
                     /translation="MSRFSINVVEVDDYSEITDILKSIRKKYLRKTIFISGSAVDYTP
                     FTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGNSIVSGVLKQRRNLRKNNIQDVLS
                     LRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLESGEIVTADGMIEEFELSVQNENI
                     VVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQALEKLDTEGEVDKKVEQIVSLIN
                     SIQED"
     gene            complement(1518..2711)
                     /locus_tag="ACHAYA_002785"
     CDS             complement(1518..2711)
                     /locus_tag="ACHAYA_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8606518.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(2795..2995)
                     /locus_tag="ACHAYA_002786"
     CDS             complement(2795..2995)
                     /locus_tag="ACHAYA_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8606519.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(3419..3652)
                     /locus_tag="ACHAYA_002787"
     CDS             complement(3419..3652)
                     /locus_tag="ACHAYA_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8606520.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(3659..4090)
                     /locus_tag="ACHAYA_002788"
     CDS             complement(3659..4090)
                     /locus_tag="ACHAYA_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8606521.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1090 a         1072 c          840 g         1342 t
ORIGIN      
        1 tgaattaact gataaaaaaa tatctactat ttaaagatac agggataagt aagtaaaaaa
       61 ataacatgtc attagatagt agacagttcc aactctttat ttaccttttt tgtatgctgc
      121 actgtcaatc caagttccta aattgttgta tccatcatca cttatataat catattggcc
      181 atcaataatt tcgtcaaacc acaaatattc tccatctggt ttttgcccaa ttattttaat
      241 tactgattga ctagtagttg tcatctggtt aaaatctttt attttaccta tattaacggc
      301 caaaatagca tttcctcgct tataactctc ctggatttca tatttaacat attctcgatc
      361 aagagtttct ttacctatca gcaccacagt cactgatgtt ccaattaatt gattatctat
      421 ccagtttttt atagcaatat ctccattacg tttaactttc tcaaattcag ccttatctac
      481 aaaaccagct tcctttgttc ctcgcaaagt ccagctgttt ctaactacca ttgctcttgt
      541 tacatctgca taatggaaac taaaaaaaac ccttctcacc atattaatcc tcctgaatac
      601 tatttattaa actgacaatt tgttcaactt ttttatctac ttctccttca gtatctaatt
      661 tttctagcgc ttgaaattta ctttttaagg aatcatcaaa ataatcactg aaattctctt
      721 gaatcttatc aaacaaaacc ttacttgcac ctttggtaaa tccaatcgga actactatat
      781 tttcattttg aacagataat tcaaattctt caatcattcc atctgctgta actatttcac
      841 ctgattcaag tttgtttcca aaaacaaaaa tactaatccc agactctgca ataatgtttt
      901 ctcgatatct tctccactca tgaggcatat caaggggaag tggtcttaaa gaaagtacgt
      961 cttgtatgtt attttttctt aaatttcttc tctgctttag aactcctgaa acaatactat
     1021 taccaattcc aagtccgtaa cctgatacta ttctataacc ggattcagaa agcctatatg
     1081 ctaatttctc gacaaatttt aatcctttcg attcagatgt aaatggagta taatcaacag
     1141 cacttcccga aataaaaata gtttttctca aatacttttt tctaatactt ttcaaaatat
     1201 cagtaatctc agaataatca tctacttcaa caacgtttat cgaaaatctg gacaaatcat
     1261 tgatttgtag tcttcttttt gtcagttcat aattatatct ctcttgatca aaaaaatcct
     1321 cagaatcgac aaaattttcc tttaaaatta tatgggattt tagattatct ttttagaagt
     1381 tcttgaatgt cggtatagcc cgtgtttccg ggcttttccg gcatttttga tattgttaga
     1441 tacttacaaa tgttcttatt acctctcacc atttccccct caaaagtagt aaatggagta
     1501 gtaaaaccgg ccttgtgtca ggctgccagc cgttccattt ctgcccttgc ggaatcgaat
     1561 gtggcgtgtg cataatagtt cagcgtcatg gtgatattgg aatgtcccat aatgtactgc
     1621 aaggctttgg ggttcattcc cgcattggct aaccgggtgc agaatgtatg acgcatagca
     1681 tggggcgtcg tcgttttcgg caacgcttct tcatggctct tgttgtactt cttcacaagc
     1741 ctgccaaaca tcccgccata atctaccgcc gtcatgggat agccgttctg tttgagaaac
     1801 aggaaattgg catacccgtc aataatgaac ggctttgcac ctttgcggtt ttttatcact
     1861 cgctggaacg cctgatacac ttcctcattc atggggattt ttctgacgcc gctttgggtc
     1921 ttgggttcgt caatatagta accgacgccg gtatttctca aaagctggtg gctcacgatg
     1981 attacccggt tttcaaaatc caaatctttg tccgtcagtc cgcacagttc cgaaatgcga
     2041 agccctgtcc ccagcagaat aacgattgcg tcataatgct tttggtaaac cttatcacct
     2101 tgaataaagg acagaaaact ttcttcctgt gctggtgtaa gaggcacctt tggttccgta
     2161 tcgtcgtcca gaacgtcact aagctggaag tcaaacgggt tttttcggat gcaatcgtcc
     2221 tgtatggcgg tgtaaaaagc cgctttcagg gaacgcttgt cgttattgat ggtcttataa
     2281 gaaaggccct tttctttcat ccgaagcgcc cattcctttg cgtcggacag cttcacactg
     2341 tcaatcgggc agctccccag ccgatcttgt tccagaatac gcatcagccg ttcccggccc
     2401 agtttggtgt tgtgcttcac attgccccgg tgcctgatct gcttggcgta gagctggcag
     2461 acggtcattt tcttgccgat ggtgtctatc ccgtcgtcaa ggtctttctg gatttctttc
     2521 tccttttccc ggagtgaaat atcctcccgt ttacctgccg gggtcttatc cgtaggcact
     2581 aacttccatg agtaaacaaa ctgcggtttt ccaaaggtat caacatattt gtaagcatat
     2641 cgtccgtctt ttctctggct ctctccagac cgcaaaacgc gatttttgct gtctcgtctt
     2701 ttttctgaca tctcgtcatg ctcctttcca tgacggaaag agccgcatat atgcttataa
     2761 attattataa catatctgcg gccctttttc actattagat tgtgtccaaa gtgtcaatta
     2821 ttttctcaaa ctgcttgcgt ttgatctgga tgcggttgcc attcataatg acccagccag
     2881 aggccggatt ttcctcggcc agcttacgca atttgttttc tccgatacgg aaatatttag
     2941 acgcttcctc aatcgtcagc gtgtattttt cccaaatagg cacatcatgg ttcataatgc
     3001 agcccccttt cacaaattca aaggtttgtg tgatttaggg gcatagacgg acggctgctg
     3061 gcacagctcc acgggacttt cacccccgcg tggttctcac gcggccctac ccattgcctg
     3121 cgacgctttt aacgctcgga ctgtggctat aagggagtat catagtcctg cctgtacgtc
     3181 gtcgcccgca agccgccacg ctcacattgc ggcgtggtgt tcctcgctcc gcttttcccg
     3241 tagggaaaag tatcatggcg caccagccgt ccggctcggc acagacagaa tgtgtccgta
     3301 tgccctatga tgcgccgccg ttatcttgcg ggcatattta tcaaggtgcg atcaggccac
     3361 cgggaaaaca ccgaaacggg gaaagggtaa gccgtttcat gctgccgggg ccggttcgtc
     3421 agttcgtgac gatagcgtgg atgagcttga tttccagacg gcgcttcatg gcctcgtcca
     3481 gacacacatg cggatagccg ttttcgtcat agagcgtccg ggtacacagc ttgttcatgt
     3541 aaccgctgta atactgcaaa acgcgctcta cggctccggc atccccagcg cgggcggcct
     3601 cgatcaccga aaggggcaaa agctcaatgc ccttgtagtt cgggttcttc atccgcgtct
     3661 aacctccctt cggcatcaac gcctttagct gtttccgcag ctccgataag gtatttgtcc
     3721 tgtgccgctg aacggcactc cgggacattc ccacaaggcc gccgatctca ccatctgcca
     3781 tatccagaac acagtgcagg attaaaatac tctgctccat gctgggcagc gcggcaaagg
     3841 cttcggcaac aagctcattg ttgatatgta ggtcatagcc atgcgacgaa aacgtaaagc
     3901 tgtcgctggg gtagtgatct actgtacaga gcttgtccaa atctgcctgt gacaaagagc
     3961 ttagtgacac ttcacggtca cggcggcgct tcatttcagc caagtagctt ttggcctcgt
     4021 tacgcagtac cgtcttgcaa aaagcatcga acatgcaccg cgcttcatag tcgcggggga
     4081 tagcatccat cttctcaccc cctttctgtg ggggacttag gtggttctct ccctttccgc
     4141 taacaagact ttgacaaggc cgccagctac ccgctaaata cctccttttt gaaaatttct
     4201 caaaaaattt ttttggggac agccgggccg caaaagacga caaaaatcgc ccggcaggtc
     4261 gcaaacctac cgggcgagaa aatgctttgc tatgctgctg atttacatgg tatagtacat
     4321 actcataacc ggaatagccc tgtg
//
LOCUS       DAYYCW010000157         4305 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000157 DAYYCW010000000
VERSION     DAYYCW010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.161"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..328)
                     /locus_tag="ACHAYA_002789"
     CDS             complement(<1..328)
                     /locus_tag="ACHAYA_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606522.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELAD"
     gene            complement(536..1462)
                     /gene="glsA"
                     /locus_tag="ACHAYA_002790"
     CDS             complement(536..1462)
                     /gene="glsA"
                     /locus_tag="ACHAYA_002790"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM8606523.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1485..2912)
                     /locus_tag="ACHAYA_002791"
     CDS             complement(1485..2912)
                     /locus_tag="ACHAYA_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8606524.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2943..4157)
                     /locus_tag="ACHAYA_002792"
     CDS             complement(2943..4157)
                     /locus_tag="ACHAYA_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM8606525.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1347 a          919 c          769 g         1270 t
ORIGIN      
        1 gatcagccag ttctgtactc gtgaatatac cattaaaata ataacttaaa aactttaaaa
       61 aataagattg accgtacaga aactgtgtga gctcagaaag agcaatacca tgtttgaata
      121 cgagtttata cttatgcttc tctcttactt gttcttttat acaatattct agacactcgg
      181 ctagactttc tagatcgtga aggatcgttg gtgtagtcac ccttagagaa atagctgttt
      241 gttcgatgtc tatttcttgg aattcaaata attgttcaca cagatacact tttctaaata
      301 tatttcgctc gatgtaattt tccagcattc ctgatacacg cctccagttt ttcttatgat
      361 tttatttaat catatctgta aatcgtattt ttgtttcctt tttatttaga aaagcccttt
      421 caaaaaccaa taaaaaaaga aactgggaca aaagtcaaac aagctcacaa tttcagcctc
      481 attgtaggta gctttgagcc gtttggactt ttaccccagc ctctttcaag aatggttatt
      541 caaagatgtc agcgtctagt tctttgacta catctttcaa caattggata ccagctgcgc
      601 tgttaccaaa tggatctagt gccgggctga agacacctat gccgtaacga tttggcacag
      661 ctgccatcaa cccgccgcct acaccgcttt ttgcaggtac accaacatgg acggaaaact
      721 ctcctgattc atcatacaaa ccagcagttg tcataattga tttgacgatt gtagcacttt
      781 cttcagtaat gatttgtttg ccattccaag gagcaattcc tttatttgcc agcacagcag
      841 ctaattttgc tatgcctgta gcggtgacat tgagggagca ttgtctgaaa taagcatcga
      901 gtatctccgg tacgtctcct tcgatcattt gattaccttt catatagtaa gcaagagagc
      961 gattgatatc acctgttctt gattcagaat gatagatttc ttcattcaat gtgatattag
     1021 gatcatcaca gatctctttc ataaatgaca agattcgctc aaatttttca tctgcatctt
     1081 taccggcaat caacgaggtt gttgcaatcg ctcctgcatt aacaaaagga ttcattggat
     1141 gtttgcgatg attgatttcc atattcatga tagaattgaa ggcaaaaccg gttggttccg
     1201 tgttgattgt ttgaaagact ttttctattc cgttgtcttt gattgctaat aacaatacgg
     1261 gaacttttga catgctttcg atggcaaagc gcacatcggc gtctcctccc tcaatcagtt
     1321 gttttgtatc caaatcataa atcgccatac ctaattgttt gggatccact tcagctaatg
     1381 ctggaatgta atccgccact tgtcctgttt gataatagtg gaaatttttt ccaatcaatt
     1441 gttctaattc ttttacttcc atttgaataa ccagctttca ttttttattt ttctgcatgc
     1501 tggaatttat gacggcatgc gtagatcgta aatggaataa ctaagacgat aagataagaa
     1561 acaatcagaa tcgtttcata aatcccatgc tgtccagctg cgatactagc aggtggaaca
     1621 aatgagatac aaaaagcaaa aagtgaagtc aaaagaccta aacaagcaac gattactttt
     1681 acaacttttc cgcctggaac ctgataggct ctttcaagac tgtcatcttt aaaaaccagc
     1741 ttcaaatagc ccgcgaaaaa cataaagtac aacatcaggt agattacaac agtcaatgac
     1801 atcgctgcaa gaaatgaaag attattcccc ccgccgctta atgtaaagac ggctgcccag
     1861 attgttgcaa cgatcccatt tgcgatcatc aaatgagcag gaacaccgtg tgtgttttct
     1921 tttgcaaaat attcaggaag taatccttct tctgctgcta cgtgaagtgc ttttgaagga
     1981 gaggttaccc aagaagcaat ttctccgatg acacctaaac aaacaagaat cgcaagaatt
     2041 ttcgcaatcc atgcaagatt tggatcaatg tgtcttaata ggaattctaa cgattgaatc
     2101 acccctgtat taagagacaa accatgttga ggtactgtag tagcgacggt caatccgcca
     2161 aaagtatcta aaatgattgc cagaataacc aatacaaaca ttgctaatgg ataatccttt
     2221 ttagggtttt ccatttcatt tgcatgactg gcagatgttt ctacgcccat atatgctaaa
     2281 ataaaggaaa caaaaacaac cagcgtaggc aatttcgaaa aatcaggaat caatgttttc
     2341 atagacagta tcgtttgtag tggagcgcct gaagcgacat aatggatccc taagccaaac
     2401 aagactaatg ctggcaacaa gataccagct acaaaaccga tccgagcaat caatgaagtg
     2461 tatttggttc cttttaactg tgagagtaaa atagcccaga aaataatcaa taggattgcc
     2521 aatttcagat atttattgtt ttgtatagca ccaatacccg ttgcatagga taatgcacca
     2581 gttaagaaat acagcatggt caagtagcca actgttatct caaaccattg gaaaaagatt
     2641 gcggcaaaac cgaatcgttt tcccaatgtg cgactgaccc aagtatagac accgcctttt
     2701 tcccatcctt tcaccgttgc catctctgct gcgcacaatg ccacagggat aaaccatagc
     2761 aaaccgccta ataacaagaa aaatactaag ctgaagcctg aggtcgcgaa tgtgggatat
     2821 tcatataatg aaacgaccat tgagatggtc atagccaaaa agccaaagaa cgttaatttt
     2881 tttgcaggaa ttttcgtttt gctttgttgc atgaatcttc ctcttttcta tttgaatttg
     2941 tttcaattga gtttgttgcc aaagaagaaa aagtccaatc cttcttcttg ttcttctttt
     3001 gtagtagtga tcgttgtaaa gtgcatcgcc gattttaaaa tcactgttgt cagcacatag
     3061 gcatacacgg cagtaatgat gacaccaatc acttgaatcc ctaagaagta ccagctgctt
     3121 ggattcgtta gtaaatggct gttagatgca aagatcccta tcagaatcgt acctgtgaat
     3181 ccgcccatgc catgaacacc ccacacatca agtgcatcat cccattgttt ctttttacga
     3241 aaatcaacag cagcatgaca gacgataccc gcaatgatcc caatcagcat ggcacttttt
     3301 gcttctacat aaccggcaca aggcgtaatg gctgccaatc cagcaacgga tcctgttagt
     3361 acgtcgacaa aatcaaagcc atttcctttt acttttgcaa tgatcagcca agtaatcatc
     3421 gcaaatgcaa gagcgatgaa tgttgaacca aatgcattga ctgcttgttc atctgctcga
     3481 agtgcaccac cggaattaaa accgaaccag ccaaaccaaa gcagtcctgt accgatagca
     3541 gctgccataa gactgctatg ctgacttctt tctcctaaag gaatctttct ttgaccaagt
     3601 gtgaggatac atgctaaaga gccgaatcca gctgtcgtgt ggatgactgc tccaccagcg
     3661 aaatctctaa atcccatctg atccaaaaat ccgccacccc atatccagtg gcaaacaggg
     3721 aaataaatca gcagattcca aaaaataaca agtataatat agccttttaa attcaatctc
     3781 cctgcaaaag ctcctgtcat caaaggaacg gtaatcacga taaacatcag ttggtaaaga
     3841 aagaacatta aaaatggaat cgttgcacca tgaaacatgt tggggaagct tgtaacatgt
     3901 ctcatcaaaa agaaatctgc cgggtttcca ataacaccgc cgatatcccg tccaaaggcc
     3961 aatccgaatc caccaaatac ccacataatc gttgttatcc caacggagag aaaagattga
     4021 gacatgatcg ttagtatatt attcttccgt gctaatccgc cgtaaaaaaa agctagacca
     4081 ggagtcatca ggcaaaccat tgctgtacat aaaatcatga atgctgtatc tcctgtattg
     4141 ataggtaatg tattcatttt tttcgcctcc tttctacgat tacgagtata aaagaaggtg
     4201 tagttgtttt tttcttctgt ttttttgaat taatacttgc atttataaaa gacagacaat
     4261 taataaaagc ttgatgtgtt gtgctctttt tcgtttttca cacaa
//
LOCUS       DAYYCW010000158         4254 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000158 DAYYCW010000000
VERSION     DAYYCW010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.162"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..1383
                     /locus_tag="ACHAYA_002793"
     CDS             82..1383
                     /locus_tag="ACHAYA_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8606526.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1410..1670
                     /locus_tag="ACHAYA_002794"
     CDS             1410..1670
                     /locus_tag="ACHAYA_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8606527.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2669..3904)
                     /locus_tag="ACHAYA_002795"
     CDS             complement(2669..3904)
                     /locus_tag="ACHAYA_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8606528.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1234 a          677 c          879 g         1464 t
ORIGIN      
        1 taattatata attagttatg aacattcttt gcgttgtcat gcaatgaatg gttcatattt
       61 tattgaaaag gagtggtttt agtggaaaag ctgatgaaat gggtcgaaga gaaacttgtc
      121 ccaccaatgg caaaaattgg tacacagcgt catttattag caattcgtaa tggggttgtt
      181 tcaacattgt cattgatttt gattgggaca ttttttatgg tctttatcaa tttaccgttt
      241 cctggatgga atgaatttat tgccccttat tcggcaacga ttgttttacc ctttaggatg
      301 acgatgggat tgatggctat ttatgcaact tttgttatgg gttcggatct agctaaatca
      361 tatggattag atagtgtgac aggtggtata ttatctttag gaacgttctt tatgttacaa
      421 gttcctgtaa acgttttaac tcctgaagag gcacctcttg gctgggtctt acctatgagt
      481 tctttgggag catctgggat gtttgcgggg atcttatcta tgatttttgc agtggaagta
      541 tatcgtttct ttaaaaatcg aaatattact atcaaaatgc cagaacaagt gccacctgct
      601 gttgcccgta gttttgaggc attgattcca ggtgcagtca ttttaacaat tacttggctg
      661 attcgaagcg tcgcgggctt tgatgtaaac gctgccttat tatcgttatt cgagccttta
      721 accaatattt tagggaataa tttgcttggt gttttactac caatgttttt gatccatttg
      781 ttgtggtcat ttggtatcca tggcatgagt attgtagggg ctgttgttcg tccaatgtgg
      841 ctgatcatgt tggatgagaa tgcaaaggca ttggcagatg gtacagctgc cacccaatta
      901 ccctatatcg ctcctgaaca gttttatcaa tggacggtaa caattggtgg tgcaggagca
      961 acgatcgtgg tatctgtttt gtttatattc ttctgtaaat caaaattctt gaaagaggtt
     1021 ggtcgttttt ctattattcc tggtatcttc aatatcaatg agcccataat ttttggagct
     1081 cctatgatgt tgaatcctta catgttcatc ccgttcaact tagttccttt agttttgact
     1141 attatcagtt attttgcagt caaattagaa atggtcaata gatttactgt tttgccaaca
     1201 tggacattac cagctccaat cggcggttat ctttcagcag gaaatgactg gcgtgttgtt
     1261 atcttgatca tgatcaacac attgatagca tttattatct attatccatt tgtaaaagct
     1321 tatgataaaa agatgctgtc agatgaacaa agtcgtggag aaaacgaagt ccagactgtc
     1381 taagtttggt ttttagaagg tgggaaacaa tgtattggct gattttattt tttgttttta
     1441 tctttttatt aacggctagt caccttatat tgaatatgct agctacatat catattcaaa
     1501 tcaatcgatg gatttgggca ctggcatcat ttttgatcgt gattcttcca aagatcattg
     1561 taccgcatat gaatgttttg ttttcatggg ggacatatgt actttgtgga atttttgcta
     1621 tcaattttat gatcgagcag catagatggt ttgtcacatc gaagctttaa atgttctaaa
     1681 agtggttgta acagaagtgg ttagcttcaa gaaataagaa gaaatttacg aaaattgctt
     1741 cttaaatttt tgtgaaatgt tggcttattt ttcgtttggt tgaacttatt accgcagatg
     1801 caagcttttg accaccgttt attcggaata tgggactgtg acaaggcttt tgtcacagcc
     1861 cttttttaca taaagattat tattgtttta actgttttgg cagaaagaag gaggaaaaaa
     1921 tggaaatttt atcagcaatg gatttagaat tagtagatct actaccaaag tgatcaagtc
     1981 aaatatagat ttgggagctt acaaccatat agtaggggct gcagtatggt gtatgaatgg
     2041 taatatgtat gatgtgagtg tttattcaaa tcatgaaacg tgtatagaaa taattgtgct
     2101 gggaatagca atcagtcatg gtgaacgtga cttttagtgt atcgttgctg acgacataga
     2161 acattctgac atgatctact caatatgtgg ggattgctgg cagtttatat aggagtatgc
     2221 atcggattgc gagatcattg tctcaacaga agagggaggt tgattatcaa agataaagtg
     2281 gtaaatccta catttgattt agtaattgtt gaggggacta gcggcacaaa tctctacaaa
     2341 gcgatgcttc tagatctata tgacacattt taagaaaatg tatttgccta attattatgg
     2401 tctttctttt gaagaaacgt tgagatgatc caaccagaga aaggtgtcaa agaatttgac
     2461 taagaataaa tggcagacgc tacctatgct catttggata gcgtcagatc ttttatttta
     2521 aggatgattt tgtgtttttt agacaccgta atttaagtaa ctctaacttt tcaatccact
     2581 atttactttt tttgtatttt cactgtctgg gtctcgaata gcgaaattac tgatgagtct
     2641 ctattaaaaa agctttttct acttgaaact atattgggtg gttgctcagg aaggtttcga
     2701 tttcttgaat tgcttgttgt ggaccattta ttggaaaaac ggcttgcagc gtgcgtgcct
     2761 gctgacgaat cttgggtgaa gaaacttcgc gcagagcaat ctgcagtttt ctttcagtca
     2821 ctggttttgt gagggcgatt gcgtttccgt attttacaca atagtcaata ttacagcttt
     2881 gctcaaattg catccccaca ccgataaacg gtttgccgga ttcacaggca gtttggacgg
     2941 tcccttcccc gccgtggata aaagaaaaat caatgaattc atgcaaaaga tgactagaca
     3001 gatattctgt tattaaaaga tcttctgagt cagattgaat gtcatcaagc agatagtcaa
     3061 taggtgcaat aaccgctaga tccggttgtt tttgtaagaa gaacaaaatt ttgcgtaaca
     3121 tacgcggatt gcctgaacta cccattgcga aatagataat acgttttctt tcttgttgct
     3181 ttcgctgtat aaaagttagc acttcttcag gcaaggaagt actgagctta gcaaaaattg
     3241 gaccaacgat tcgataattt tccggcagct gttcttcttc caaaaatagt ttaggcgttg
     3301 tgatgagatt tagatcagca tccatcattt gtaaacttgt ttttggtaaa ggaagcattt
     3361 ctttttgtgc agcgtgtttg aaggcattgg ggatccatcg ggtatttact atacgctttt
     3421 ttgtccagtg ccaaagggga ttgcttaaag ggccgaagaa acgtaactcg tggggcatca
     3481 gcgcatgctc tgattgaaaa aaaggttcac tcaaagcata gggtttaaca tatactaaag
     3541 gaatcttttt attgcgggcc gataaaaaaa ttgtcacatt cgaacctgtc acaatcagtc
     3601 gaggctcaaa cttagcaatc agactctgtt cactagtgat acgagaggca accatttctt
     3661 tggtaaaagg gtttttaagt gtacgcaact ggtcaaattt tatgatttgt tcttcttgtt
     3721 ttttcgtaag gatcggcgct agtaaatgat actcaaagcc actttctgtg atcaaaggag
     3781 caaaagtttt agaaaagcca aagaatacac agtaataacg gtctttcata gctttggcaa
     3841 cctcaatcat tcgtgttgtc tcagctaaat tgaatactgc aggagcaaaa agaattgtct
     3901 tcatgaaatc ctccttattg gaatgagtct atcccttcgt ttcttactag tatactaaaa
     3961 aacacagcgt tcgagtagcc tatgtatatt ttgaatatta aactgtctct cgctgttttc
     4021 acaacttcta aatttaaatt ttgtaatcaa gttgaaactc caattttcaa aaaaacacgc
     4081 aacaattttt tgatcgattt atatgttgtg ccagataaat acgagaagtt gtgtcattaa
     4141 aaataaacat gagaacataa aaaaataaaa taattatatt aattgatagg tgtgctgtcg
     4201 tcattataca cacgtaagaa agaaatagtt atgtttaaaa atattcttag actt
//
LOCUS       DAYYCW010000159         4179 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000159 DAYYCW010000000
VERSION     DAYYCW010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.163"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(120..1886)
                     /locus_tag="ACHAYA_002796"
     CDS             complement(120..1886)
                     /locus_tag="ACHAYA_002796"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8606529.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1846..3600)
                     /locus_tag="ACHAYA_002797"
     CDS             complement(1846..3600)
                     /locus_tag="ACHAYA_002797"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8606530.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3710..>4179)
                     /locus_tag="ACHAYA_002798"
     CDS             complement(3710..>4179)
                     /locus_tag="ACHAYA_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM8606531.1"
                     /translation="HSISPGIFGLLSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMG
                     GAYLPWFISSISMAILADIILSVFGYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRF
                     RQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         1403 a          790 c          590 g         1396 t
ORIGIN      
        1 tcaatcatac tgattctttc gtgtattcaa aattagatgc tgtttaataa aattattctt
       61 tttgaaaaag aattataact atatcccaaa aattgtaggt taatttaaag cctatttcat
      121 catcgtgatt gagcttcttt ttcatagacg aatttctcag atagtttagt tttctttatg
      181 agatttttgt atatttctga ccttcgatac aactcattat gagtaccttg atcaactagt
      241 tttccttgat ccatcactac aattttattt accttttcaa tagacttcaa gcgatgcgat
      301 ataattagta ctgtcttatc ttgtattaaa cgatttaaac tttcctgaat acgtttttca
      361 ttttccgcat ctaaactggc agtaatttca tctaaaatta aaattggcgc atttttcaaa
      421 aaagcccgtg ctatggatag gcgttgtcgc tctcctccag aaagtgaact tccgttttct
      481 ccgaccaatg tatgataacc gtcaggtagt ttttcaataa agtcttcgca gttagctaaa
      541 cgagctgctt ttttcacttc ctcatctgac gcttgactat ttccaatacg tatattttcc
      601 aaaatacttg tattaaacaa agttacctct tgaaaaacaa tagcgatttt gctaaacaaa
      661 gaagctggag atacacgttt aatatcatac ccatcaatta aaatacagcc ttcatcataa
      721 tcatacagtc tagacactaa tttcaagata ctagttttcc cggaaccact agcacctact
      781 aacgcagtca cttctccttg tttagccgta aaagagatat gatctaaaat tggggtattg
      841 ttatcatatg agaaagagac atctcgcaac tcaatgtcaa aagatttcag aggactatct
      901 gatccctctt ggatactggt ttccttcata gctctaattc gctgtatttt aggagctaaa
      961 taaaatattt caagcactgc ttctttcata gaatcgaagg aatcctttat ttttattgcg
     1021 gctagtaagt aaccaacgac gtagagtata gtcacttcac ccgttaacag taagtgaaca
     1081 cctactagaa tgactactgc taaactaaca tatgagaaaa tagaggataa ggccatgact
     1141 gaaaaagtac ttaattctac ctttaaatga atcctttcac tctcttccat ttttttgaaa
     1201 agtctgtctt gaactttttt agatagatta aagctattaa tctcttgctg caattctata
     1261 gtttcttgaa attcttccga gttctctctc aaagtatcgt aatatttagt attggctttc
     1321 gtttgggatt tctttgataa caagattaag acaaaactaa ataacgttgg caaaataaca
     1381 gctaatccca ttttgacatt accaaccaaa agcatcactg aaataaaagg gaaaaacaga
     1441 gccataccgc cggctttagg tattgcatga ctcatcgcat gctcaatacc ttcaacgtca
     1501 gacatgatag tttgagataa atctgataaa ttatgttttg aaaaatagga taatggtaaa
     1561 tttgacaaat cgtctgcaat ttgcactctt aaatgcgcac tttctttata agtactatta
     1621 tataagtttt catactccct atctaacaac caatacatga aaagcaaagt aacaaacgat
     1681 acggcaaaat aaagccaacg gggctttgta ttttctaaaa ctaattcgtc tatcagcatc
     1741 attataatga atgctggtac catattaatg cagtatacaa aaaaactaga aatagaagct
     1801 ttagttaaag cttctgcgcc ctgatcactt aaagcaaatt tgtttttata aaaacttttc
     1861 attgtcaagc ctccaatcat ttgcattctc ataattagtg accaattgtt tatacacttg
     1921 atgtttagcc attaattcat tatgatttcc acgttctact attttccctt ctgacaagac
     1981 aagaatttca tctacagatt taatgctgga taatcgatga gctatcataa taactgtttt
     2041 ttctttcatc agatgttgaa aagccttttg tagttcatac tcattatctg gatcaataga
     2101 tgcactggct tcatccataa taacaatctt tgcatctttt aaaattgctc tggcaatagc
     2161 tatacgttgt ttttctcctc ctgacagata aactccttta ctaccaatta aggtatgctc
     2221 tctttcggga aatttttcta aaatcgattc gcaaccggct aatcttagcg cgtcaaatac
     2281 tttacttgct ggcgcttgag gatttgctat agaaacgtta tcgtaaatac tctgattgaa
     2341 tagtttcgta tcttgaaaga caaaagcaat tgtgttaata attgtgtctt ttgaatagtg
     2401 atttaaaggc ttaccaccaa ttttaatctg tcccttatcc acattgtaga aacctgaaat
     2461 tagtttagct atcgtactct ttccgcttcc tgattttcct actaatgcat aaattctttt
     2521 ttgttctaaa actaagttga aattttctaa aacacactct tctttgtatg aaaagctgac
     2581 attttcaaat gcaatgtcac aatttttaat ctgtgtctcg ttcccatatt ttagttggtc
     2641 ttgcttcatt tctaaataaa gggagtctag tgtttcgact gcatatcttg cttcaaacaa
     2701 gtgcatactt acatacatta ttctcatgaa agaaacaaaa ataattccac ttaaaaaaaa
     2761 caacatcaac aagtcaacag ctaatatctt gggagtggta agagaagtta taaaaaaggt
     2821 tataggaact atcaaaatgc aaactatgcc aaaaaatagc cattgaaaaa gaacaaaagg
     2881 tgttttccca ctcaaagaat acttatatgc aaacttagca taatcgttaa ttgctttatt
     2941 caaggcttta aatgatgtta ctttagcacc aaatatcttt ataacttgaa ttccgcggat
     3001 atattctacc gtttcagagc ttaacacatc taacgattct tgatattttt gaataaaatt
     3061 tgtattctct gtcattttct ttaacaagaa tacaccaatt actaacatgg cgattagaac
     3121 caatccaatt ttaaagttaa tgaaaaagcc taaggcaact gataaaacag gtgttactat
     3181 ggcttgagca ctatctggaa tcaagtgagc aattgccata tgtgttttag ccgcgttgtc
     3241 atcaattgtc tttcgaatat atcccgaaga atttaaatca aaaaaacgaa aacttgcttc
     3301 tgttaaaccg ctaattccct tttttctcaa attagtctct aagcgaaacc caaggatatg
     3361 agaaaataag ccagaaaaaa tataaaataa agctcctaaa gttaaccaag caacagtttg
     3421 aaccgcataa ctcttagcta aatattcatc tcctacaata attagagact gaagaaattt
     3481 gtaaatactg taatatccat agactgttaa tacgacagaa aaatcagaaa ataaagtggc
     3541 taaccatccg tagtatttca ttttagggac ataactaaat aattttttgt atactttcat
     3601 tttttcctcc cccgcattca atttcttctc ttattcgttt acaactgtta atgctttccg
     3661 taatagcgta aattagagat gtacaaacag ataaaaggta aactacgttc tattttttgt
     3721 atttttttaa gacttttctt ccaatcaata caccaatcat agataaactt gctacaacta
     3781 acactgaaat gattccccat atacctaccg catagtggat cattgcatcc attgttgcag
     3841 cagattgtcc tttatctatc caatcttgac gaaaacgatc tgtaaagaac catataggaa
     3901 tccactgtcc ggcagcactg cctaattgca ttagagccca agacgcaact tgaggaattg
     3961 ctcgatcata tccaaataca gatagaataa tatcagctaa gatcgccata ctaatcgaac
     4021 tgataaacca aggtaagtac gccccgccca ttaacgtaaa aaataacaat aaaacaattg
     4081 tgtaaatagt aaaaattcct tttctagggt agttatagct aatataaaca aatatgattc
     4141 cagataataa tccaaaaatc ccgggggata tgctgtgac
//
LOCUS       DAYYCW010000160         4169 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000160 DAYYCW010000000
VERSION     DAYYCW010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.164"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1003
                     /locus_tag="ACHAYA_002799"
     CDS             <1..1003
                     /locus_tag="ACHAYA_002799"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8606532.1"
                     /translation="ILIRVRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAV
                     LMAGQYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAR
                     EFGAMTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSK
                     KWETCLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAAC
                     WFMEMQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNK
                     LMVLDQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            985..1446
                     /locus_tag="ACHAYA_002800"
     CDS             985..1446
                     /locus_tag="ACHAYA_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8606533.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1496..1960
                     /locus_tag="ACHAYA_002801"
     CDS             1496..1960
                     /locus_tag="ACHAYA_002801"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8606534.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            1974..2723
                     /locus_tag="ACHAYA_002802"
     CDS             1974..2723
                     /locus_tag="ACHAYA_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8606535.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2723..3553
                     /locus_tag="ACHAYA_002803"
     CDS             2723..3553
                     /locus_tag="ACHAYA_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8606536.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3553..>4169
                     /locus_tag="ACHAYA_002804"
     CDS             3553..>4169
                     /locus_tag="ACHAYA_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8606537.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLR"
BASE COUNT         1422 a          547 c          803 g         1397 t
ORIGIN      
        1 aatacttatt agagtgaggg ataaagaaat gagacaacta cacaaagaac agattgaaac
       61 agcaatggca gcgttaaagg aaaagacaat tgatcatgta tattttacag catgtggggg
      121 ttcacaagcc gttttaatgg ctggtcaata tctatttgat aaagaattaa ctgtaccttg
      181 tcatgtttat actgctaacg aatttaacta tgctatacca aaaggattat ctgaaaattc
      241 tttagttatc acttgttccc attctggcac aacaccagaa actgtagaag caacaaaaaa
      301 agcaagagaa tttggagcaa tgactatttg tttatctaat gaagaaggat ctcctctatg
      361 ggaagcagct gaatatcctg ttcactatga ttggggtaaa gatgttgatg ctagtgattt
      421 aaataaagga attctttatg gtttattatt taatatttta cttgttttag atggctctaa
      481 aaaatgggaa acttgcttga atgaatttga taagttaaat gatcttgtta ttgctgaaaa
      541 gagcaaattt gaaataactg ctaaagaatg gggtaaaaac caaaaacgag ataaaattat
      601 ttacacaatt ggtagcggta tcaattacgg agaaatttac tccacagcag cttgctggtt
      661 catggaaatg caatggatta attctaatgc tattcattct ggggaatttt tccatggtcc
      721 ttttgaaatc actgattatg atgtaccatt catcttagtg aaaagtattg gagatacaag
      781 atttttagat caacgggtag aagacttcac taaaaaattc agtaataaat taatggtttt
      841 agaccaagct gattttgact tagaatctat agatagtgta gcgaaagaat atattgcagc
      901 tgtacttagt ggcgttgtta ttcgccaaat ggtagaagcg attgcttttg aaagaggcca
      961 ttccttagat gttagacgct atatgtggca aatgaaatat taattaatag gaggttgcaa
     1021 aagatggcaa atcaattatt tcacattgtg attacaacac attctaccct ttgtgagggt
     1081 tatttaaagg caacaaaatt aattttaatg aatgacgcca caggtattag tgctgttcca
     1141 tttaaagaaa atactagtcc agatgatttt gaggagagta tgaaagaaat aattagtcag
     1201 catcatttgc aacctctaat tattttaacg gatttaattg gaggaacacc taataatgtt
     1261 tctattaaac agttgaacaa caatagaatt caaattattg ctgggattaa tttacctctt
     1321 ttactggagt tattaatggc ccagtcagct ggtaaatcta tagaagaaat agattttaag
     1381 gaattactga ataactctca aaaaagtatg gttcatatta attcgctttt aaaagatgtc
     1441 agttaaggtg gcaatttcta ttctataaat tttaaaaatt gaaggaggaa atagaatgat
     1501 taaattagtg cgtgtagacc acagattact tcatggacaa gtaatatttt cttggactaa
     1561 acagtatgat attaaccata ttattgtggc tgataacaaa gttccaggag atccaatgtc
     1621 tgtgatggct ttgacaattg ctaaacctgc tgattgcaaa ttagacattg ttaaaattag
     1681 tgatgttaaa gggattatgg aaacatcccc aaatgataat tacatgattt taattaaagg
     1741 acctaaggaa gcttttgaat tagttcaaaa tttaccagaa attaaagaaa ttaattttgg
     1801 cggtgtgtct aagaaaagcg agtccaaaca atatggaaag gcagtctttt taactcctga
     1861 agaattagaa gccacaaaaa aaattcttga tcaagaagtg aaaatatttg tgcaacaagt
     1921 cccatcttca agtattgaaa atgtagattt tagtaagtaa ggggggaaaa taaatgttat
     1981 taaaagccat tttattaggt ttagttggtg tggtagctgt tattgactcc aggttaattg
     2041 gtcgacaaaa tattggtaga ccactaattt tgagtacact tgccggactt gtgctaggtg
     2101 acttgcatac agggattgtt cttggggcat ctttagaatt aatttctatg ggattcgtct
     2161 caattggggc ggctggacct cctaatatgc aacttggtag tattattgcc actgcttttg
     2221 caattttaac taatagtagt acagaaacag ccttaacaat agctatccca gtggctgtag
     2281 ctggtgaatt tatgagtatt atcatgcgca tgataattgc tcaatttgga catgctgcag
     2341 ataaagccat tgaaaataat aaatttagaa aagctcaaat tattcatatt tattggtcat
     2401 ttatttttaa tgcttttgtt tactttattc caatcttttt aacagtatat tttggtgctg
     2461 acgttgtggc agatttagta gataaaattc cagaaatgat tacagacgcc ttgactgtct
     2521 ctggaaatat gctttccgca ttagggtttg caatgctatt aagcacaatg ttgagtaaaa
     2581 aattatatcc ttactttatt tttggattct ttattgttgc ctattcaggt ctttcactaa
     2641 ttggcgtgac aatttttgca gctctgattg cctttgtaat ggatcaagtt aaatatggaa
     2701 aaagagaaga ggcgcatggt taatggaaaa tagtacagaa aaaaaacgtt taatcacaaa
     2761 aaaagatctt aataagatgt gtcttcgttc atggacaatc atgtgttcgt ggaattatga
     2821 aagacaaatg catatgggat ttatgtatgg gatggcgcca attttaaata aagtttataa
     2881 aaatgatgaa gagaaaaaaa gagaagctta tactcgtcat atggcatttt ttaactgtac
     2941 gcctcaaatg acttcattta ttatgggact ttgtgcttct atggaagaac aaaatgctca
     3001 agcagaagaa ggaaacttta atactgaatc tatctccatg attaaaacca gtttaatggg
     3061 tccttttgct ggaattggtg attcattttt ccaaggtact ttaagaatta ttacatttgg
     3121 gattggattg tcttttgcta agcaaggtag tattttagga ccgattttag ctgttggcct
     3181 ttttgctata ccttctattt tagctgtttt ttacggcggt aaattaggat atgagtctgg
     3241 caataaatac cttactaaat tgtatcaaga aggtcttatg gatcgagtaa tgcaatttgc
     3301 ttctatcatt ggtttggctg tagtaggtgg aatggtggct agcatggtgc caattgtcac
     3361 ccctttaaaa ttcactacag ggggaacaga acttgtttta caagacttac ttgattccat
     3421 tattcctgga ttattaccac ttgtatgtac tttgggaata tataaattga ttaagaaaaa
     3481 tgttagtaca aatatgctct tattaggttg tattttggca gggtttgttt taagtataat
     3541 aggtatctta taatgattaa ggctctaatt tttgatatgg acggagtttt aattgatagc
     3601 gagattatgt atcaaaagtg ggtatttaat tttttaaaac aagaaaattt aagctatcct
     3661 ttgtattctt atcaaaaaaa gattggaact acagcaagta tttttgatga tatagaaaag
     3721 tacaatgagg gatgtaacag tttagaattg aagggaaaat ttactaaata ttggaaatcg
     3781 caaaagattg attacttgga tatttttcct aatatactaa ttgaacagct tgaatggtta
     3841 aaaaagaaaa aaataaaaat ggcaattgct tcttccagtc caatggatac tataaatgaa
     3901 gttgttacca cttgtaaaat agagtcatat tttgattgtt taattagcgg aagagatttg
     3961 ccagaaagta aacctaatcc tactatattc cttaaagctt cagaacagct cagaattcca
     4021 gttgaagaat gtctggtgat agaagattct tataatggta taaaagctgg taaaagagca
     4081 aatatgaagg ttcttgcaat taaagataaa cgtttttctc aagatgtaag tctagcagat
     4141 gatgttattt acgatattaa atatctgcg
//
LOCUS       DAYYCW010000161         4067 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000161 DAYYCW010000000
VERSION     DAYYCW010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.166"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1157)
                     /locus_tag="ACHAYA_002805"
     CDS             complement(<1..1157)
                     /locus_tag="ACHAYA_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8606538.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
     gene            complement(1160..3409)
                     /locus_tag="ACHAYA_002806"
     CDS             complement(1160..3409)
                     /locus_tag="ACHAYA_002806"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8606539.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            complement(3506..4009)
                     /locus_tag="ACHAYA_002807"
     CDS             complement(3506..4009)
                     /locus_tag="ACHAYA_002807"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8606540.1"
                     /translation="MENSIYLVTEPNTQLKDQDNLVSELSRQKQVYKFLELLLNSASY
                     LNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIK
                     EYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLG
                     LGIINGE"
BASE COUNT         1311 a          744 c          580 g         1432 t
ORIGIN      
        1 actctagtat tttttttatt tgtcgcaaaa aatgttacaa ataggatcac aataaatatt
       61 cctgcgaata caaaaaatgg agcagtcaaa tttgtacttc ttgttagaaa ttgtgctaat
      121 tgcatagcaa taggagctat aaaattacca atattacagc caataaaaat caatgaagta
      181 gcaaaattaa ttgtattctt tgtagtgact ttatctaagt tactaaagat aaatgggaag
      241 caccatgatc ctgttatact tgataaaaat aaacccgtta gcaggaacgt catattaccg
      301 tttgacacag caattaacaa atttgaaatt ataaagaata taattcccaa gtatagggtt
      361 ccttttccta tccacttatt tattggtcca aacataaatc ctgctagtat tcctaagata
      421 ggcattagag ctaaatagtt actagcatta aagtttaccc ctttaatttc agttgcaatt
      481 gatgcaaatc gtacagaaat agcaatacta ttcattacga gtaagattgc aaatagaact
      541 agggcaaaaa ccattggatt catcttttct tttacatgat tttcaggtat gtcactattt
      601 tcatctctaa tttcaggaac ttttagcgcg aataagagag ctacaggaaa cgcaattgca
      661 tagactaaaa acgaagccgt ccaattaatg ttcactaaaa ctccagcaag aaaaataagg
      721 attgtctgac ctaaagcttc cattgaattt ctaataccta atagcgttgc tctagtattg
      781 ccagagtata aagagctgat atatgtcaca gccaatgaat tatatactcc taaaccggca
      841 cctaatatta ctcgactaat aagaacggtt tggaaggtag ctgtaaatac tggaataatt
      901 cctcctatac cagctaataa taaacctaaa attattgtct ttttcattcc tattcttttg
      961 gcaaagtaac tagataataa gataaacaaa ataacagtta tacttggtgc agttcctaaa
     1021 agttcacttt gactttgtga tatccccaaa gactgtttca tttgtggaat aacaccatta
     1081 attgcttgag agcttgttaa cattagtgaa atacttaaga ttgctccttt ttgaagtaag
     1141 cttttttggt tattcatctc tacttttcct cctgtgttat aacccaaatc ttggaatcaa
     1201 aatcaaatgt ttctgggcgt cgattctgat catttatttg tccactagat gcatctgaac
     1261 agactagtcc tgctctcatc aattccattc cagatcgcaa actattttgt cctacattat
     1321 aaagtccgtc attatctaac ccttttaatt tcagtcggcg gtaaggagca tttacctcat
     1381 ttaatgtttt ataatgagca acaatagccg ttttcttatc atgactaaca accatccacg
     1441 cggtttgatt atcatcatcg aatggtgata atagtctata aaaagtacca ttatgaatta
     1501 tggttctata ttttttataa aaggcaattt gttcttttac aatcctcaaa tcgccttcac
     1561 ttaattcatt taagtcaagc tcatatccaa atgcaccaaa aaaagctaca tttcctctag
     1621 tttttatagg tgttatacgg tttgtttgat gatttggaac aattgataca tgagcaccaa
     1681 tagacgaaag aggatacagc atactagttc catattgaat ttttaacctt tcaattgcat
     1741 ctgaatcatc actagcccaa gcttgaggtg catagtgaag cattccagga tcaaagcgcc
     1801 caccacctga tgaacaagac tcaaatagta tgttaggata ttttgttata agtttttcat
     1861 ataagcgata tacgcctagt acatatctgt ggaatacttc tccttgttga tctgctgaca
     1921 agtgactgtc aaatacatca gtcaatggac gattcatatc ccacttaatg taatctaagt
     1981 ttgattcatc aatgatttta cacatttgat cgaaaatatt ttcaacaacc tcatctaacc
     2041 caaaatttaa tacatattgg ttcctaccat gattaggttt tcgattaggt gtagaaataa
     2101 tccaattcgg atgtgtgcga tataactcac tatccttatt caccatttca ggttcaaacc
     2161 ataaaccaaa tttcatacca aaactacgaa ttttttctga aaggctgccg attcctctag
     2221 gtagtctttt aggatttaca tgccaatctc ctaatcctgc acaatcgttt tctctttctc
     2281 caaaccagcc atcatctaat acaaataatt caataccaac atcaaatgct tttttagcaa
     2341 tatttaccaa cttttcttcg tcaaaatcaa aatatgttgc ttcccaatta ttaattaaga
     2401 caggacggtc tttttctcgc cacttaccac gagctaagcg ttttctaaat agttgatgaa
     2461 aagtttgact catggcattt aaacccttac ttgaataaac cagaatcgct tctggtgctg
     2521 taaatatttc tcctggattg agtttccaat taaatccaaa tggattgatg ccaacttgta
     2581 gtctagtaac atcccatgta tctacttctg cttgaattaa gaagtttcct gagtaaacta
     2641 gtgctgcacc aatagcttct ccttgatttt catcagtatt ttttcttttt aaagtaacaa
     2701 atgggttatg ctgatggctt gaaatacctc gagtagattc gattgattga attccttgtt
     2761 gtaaagtacg cgacttaata gttctttctc taccccaagc accagataat tgttgccatt
     2821 cataatttgc atcgggtaaa tccaatgata aactgctaat attctcgatt atttgaatat
     2881 cttttccttt attcataact cgcgcgctac gagtaataac aggataatct ctataaattg
     2941 tatataataa ttctacttga acatttgtaa ctgaatctaa aatataaact ttcaatgttt
     3001 tgctttcatc attattatca acataggtag caggtaaacc ttctagttta ggttttcctt
     3061 cttccacttc gtacccttga tagacaaaat ctgataagcg agagccattt ttctgtagta
     3121 atgaaatagc aggatgtcta aaatctgttg taccatattc aggaaattct tgttttaagt
     3181 gctctaacga atatagcaaa tcgttttcat ttctataggt tgtcataggc cgatgatgca
     3241 tctcaattag atgactatag tcattatctg gtaagttctc accataatat aaatgcaata
     3301 atttaccatc ttccgatatc ttaaaaatgt aacttatctc ctgattaaac aaatgaaatt
     3361 gttggttttc tgatcgataa ataatattca tttcattatc cttatacata agctctcctc
     3421 cgtttcttta ttgcattata aaaaacgctc acataaatag taagcgttta aagtaacgaa
     3481 tatttatcaa aatcaaatgt ttagtttatt caccattaat gattcctaga cctaatctac
     3541 gttttcgata tactcccggt gaaacaccat ttgctttctt aaaggcttta gaaaaagatt
     3601 gtactgttga gaatcctatc ttgtcactga tttgctggat tgaatattca gttgatgata
     3661 acagatcgtt tgcaatagaa attttgattt cactaatata ctcttttatt gatattccca
     3721 tatcattctt aaacaatcgt gataaataat tttcgtgtat cgatagactg acagcaatat
     3781 ctttaacaga taatttttga tacgcattct cacgtattat ttcaattgtt tgacgaacaa
     3841 aaggattcat ctttttagta attatccctt cgttcagata actagctgaa tttaagagga
     3901 gttctaaaaa tttataaact tgtttttgtc tggacaattc actgactaga ttgtcctgat
     3961 ctttcagctg agtgttaggt tctgttacta aatatataga attttccata tccaccctcc
     4021 tatgttagat ttggataatt tttgttttat tatgtttata ttattac
//
LOCUS       DAYYCW010000162         4004 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000162 DAYYCW010000000
VERSION     DAYYCW010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.167"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(92..550)
                     /locus_tag="ACHAYA_002808"
     CDS             complement(92..550)
                     /locus_tag="ACHAYA_002808"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM8606541.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(721..1866)
                     /locus_tag="ACHAYA_002809"
     CDS             complement(721..1866)
                     /locus_tag="ACHAYA_002809"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM8606542.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(1879..2796)
                     /locus_tag="ACHAYA_002810"
     CDS             complement(1879..2796)
                     /locus_tag="ACHAYA_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM8606543.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3224..3787
                     /gene="efp"
                     /locus_tag="ACHAYA_002811"
     CDS             3224..3787
                     /gene="efp"
                     /locus_tag="ACHAYA_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM8606544.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1252 a          751 c          766 g         1235 t
ORIGIN      
        1 ccgtttagaa ctcttttcag acaataaata tttaataaaa tacgaagata agatagctat
       61 attgcttctc atctttacgt tcttttagaa actatgcttc tacttcagac cattcatcgc
      121 gttttgctaa aaattcacga gccaaagctt gtgcaccttc tagtgtatgg ctggcagccc
      181 agccacattg tgtttcattg cttgcaggta cttcagtagc ttcaagaaca tctttcattg
      241 ttgcttcaag aacatctaaa atgtcttcgt agctttcatg attcaataca gtcaagtaaa
      301 agcctgtttg gcatcccatt ggagaccagt ctacgatgta atctgcgtga ttgcggatta
      361 gttcggcagt caagtgctca agtgaatgta gacttggcat gtccatgtgt tctttatttg
      421 gttgtttaaa gcggacgtcg tatttaatga tgacatcgcc atgcgcaccc gtttttcgat
      481 cagctacacg tacataaggt gctttgactt ttgtatggtc aagattaaaa ctttcgacat
      541 tcattctcat ttgtaagact cctatctgtt tgttcattcg ttttgatgct tttcactaaa
      601 gaattattta gtgaaattta caaaaacgtc tttctacttg attctaacac ttatcgtttc
      661 caaggtgtag caatctgaaa tattttttag atttttctgc taagtcgttt attgggactt
      721 ctagttcttg attctgtcaa atgcctgagc taaatcgttt atgagatcct gaccatactc
      781 aattcctaca gaaagacgga tcaagccgtc ctttatgcca gcctcttctc gtttttcttt
      841 tggaatagac gcatgagtca tgacagaagg aatttccact aaactttcta cgccacctaa
      901 actttctgct aaagtaaata gttggagtga ttcaacaaat ggaatagcag attcttcatt
      961 ttttaaagta aaagaaatca ttccgctaaa gccgctcatc tgctgcttag caactccatg
     1021 gagttcatga tcgggtaatc ctggatagta gaccttttct actgctggat gagaaaatag
     1081 aaaatcagct accacaaaag catttttttg atgttcctcc atacgaacac ttagtgtctt
     1141 gattccgcgc tgaagtaacc agctatcttg gggaccaagt acggctccga tagcattttg
     1201 gtaaaatccg atctgatcgg ctagatcttt atggttagaa gtcactaaac cagctactac
     1261 atcgctgtgt ccgcctagat atttggtacc gctatggaca acgatatcag aaccgagatc
     1321 taaaggtctt tgaaaataag gagtagcaaa tgtattgtca gcaataacta tcaatccatg
     1381 ttgatgagcc agagtggcac ttttttctaa atcagtaatc tttagaagcg gattgctagg
     1441 tgtttcaaga taaagtgctt ttgtattggg tttgatggat tgttctattt tatcaagatt
     1501 actcgtatct atgatcgtgt actcgagacc atttttagta agaactttgt caaatagacg
     1561 gaatgttcct ccatagacat catctcctaa taagatatga tcgccagctt gaaaaagaga
     1621 aaaaacggca tgaatcccag aaagaccaga agaaaaagcg aatcctcgaa caccgccttc
     1681 caaatcagca atcagttctt ccaatgcaaa acgagtagga ttaccagatc ttgaatattc
     1741 atattgttta ggctggccga cgccatcttg ccgataagtg gatgtttgat aaataggtac
     1801 actgacagct cctgttgtgg gatcttcact gatgcctccg tggatcaatt ttgtttgaat
     1861 gtgcatgttt ctttcctcct ataagtaaat gttttttgat aaatagcggt ctgctgcgtc
     1921 tggaaaaatc gtaacgacac gatgaccagg tggtaaacga cgaatttctc ttaatgctgc
     1981 tgcaaaagcc gcaccactcg aacttccaac taatagtcct tgttctcgtg ctaactgcct
     2041 agtatagtta aacccttcta catcagagat tgtctcgatc tgattgattg aaaggggaga
     2101 aagaaacggc ggaatgaatt ctacaccgat accctcaatt tcgtgagggg ctggatcacc
     2161 accatttaac acagagccct ctggttctac accgattatt cgaatgtcgg gatatttttc
     2221 tttcaaaaag gtagaagttc cagcaaaggt tccaccactc ccaattcctg ctacaaaact
     2281 atggatattt tctttgattt catgaaagat ttctggccct aacgtatgat agtaagcagc
     2341 aggattatct ttattttcaa attgaagagg cagatagctg tttgatatct cctctgcaag
     2401 ttttttactt ttgctgatgg caccgatgat tccttgctca ctaggagaat ggatgatctt
     2461 tgctcccaat gctttcatca attgttgttt ttctaagcta aacttctctg gaacaacaaa
     2521 aaccgttctt aatttgtatg tgagtgcggc cagagcgatc ccaattcctg tatttccggc
     2581 agttggttcg atgatcgtcg ttttttcatt gatttttcct tgggcaaatc cttctttgat
     2641 caaataactg cccaatctat ctttgatact tcctccagga tttaaatatt ctagttttgc
     2701 ataaatagca gaacctttag gcatagaata gtgctgattg tcaaagcgat aaagcggtgt
     2761 ggaaccaata gcttctgtaa ttgtcgagat aatcatgtgg gcctcctaaa aaaagataat
     2821 aaaaaaagga gctgactatt aaatcagctc cttgaaagac atagagaaaa attgagtaga
     2881 taaaaagata agttacctgg tactcattct atgaagggca attaatagac atgacaaata
     2941 gtatgctgct tttatgcacc atacaacagc aacgattcaa ttgtttttgg ttgctgactg
     3001 tcatgtgtat ccctccaagt aaaattttta ttaacgatag catgaaaaaa aataaaagtc
     3061 aaaggcttga aagaaaaaaa tatttcgata gcatcgttta cccgtgttta tttttctgac
     3121 aaataggcat gagaccttaa aagatagaga atactctagt tttctggccg gttgtctgat
     3181 acaataaagt tgattatgat aaagaaaaga aggagcaaaa ataatgatct cagcaagtga
     3241 tttaagagcc ggtatgactt tcgtacaaga cggcaaactg attaaagttc tggatgcaag
     3301 ccaccataaa cctggtaaag gaaatacagt tatgcgtatg aaactgaaag atgtccgttc
     3361 aggtgcaaca tacgacacaa cgttcagacc agaagaaaaa tttgaaaaag cacatatcga
     3421 cacaaaacct gtacaatatt tgtacacgat ggatgataca gctttcttca tggatttgga
     3481 aacatatgag caatatgaaa ttcctgtaga aacagtagaa gaagaaatga aatatatcct
     3541 agaaaacatg gaagtaaaaa tccaattttt cggtagcgaa gtcatcggcg ttcaattacc
     3601 aacaactgtc gtattacgtg ttgtggaaac acaaccttcc atcaaaggtg caacagtcac
     3661 tggttcaggt aagccagcga caatggaaac tggcttagta gtcaatgttc ctgacttcat
     3721 cgaaacagat gaattgttgg aaatcaatac agcagaaggt tcttatgtaa aacgtgcgtc
     3781 aaaataattc gctaacggat tgataagaat ttgaaaacag agcttgacct cttgaaaggc
     3841 agatcatcgg atctgccttt caagaggttt tttgtggaat cagcaaattg aaaaataaaa
     3901 aagtatttga ttttatttat attttgaaag atgaatggca aaagctagag aaagaaaaaa
     3961 ctgcacttat ttatgaagaa attgaaatga ttcataaaat tgaa
//
LOCUS       DAYYCW010000163         3877 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000163 DAYYCW010000000
VERSION     DAYYCW010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.168"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(717..3866)
                     /locus_tag="ACHAYA_002812"
     CDS             complement(717..3866)
                     /locus_tag="ACHAYA_002812"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFM8606545.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          636 g         1362 t
ORIGIN      
        1 gactaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
       61 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
      121 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
      181 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatatacag
      241 tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat
      301 tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
      361 tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt cattacagca
      421 aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa
      481 acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg
      541 gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata
      601 gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc
      661 tacacttcca ctttactacc atctttgaat cgtacaattt tcttaactct tctcgtttac
      721 ctcaattcat tttcaagtgg aatcagctca tcatctaaca ttttacgcca tgctcgcttc
      781 atttcttgtg ggtatttaaa tggcttcgct tctggatggt tggctttata tccttcgtaa
      841 tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg catatattgc
      901 agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc
      961 aaatcatcac tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta
     1021 tctaaagtcc ccacatcaaa cgcatttaaa atttgtcggt aaacttcttt gactccttct
     1081 gttttattca atttagccaa agctttttca atttcttctc gtgcatctga actattcgcc
     1141 gcataagttc ctagtaattg atcaatataa gtagaatcaa tatcatacaa cttcgtggta
     1201 ttttcactat aaaattcgat cgtggaaaaa tcttctccct ctttttctgg ttcttcctct
     1261 attaatgaac ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga
     1321 ttgttcaagg ttgaagattt caccatgtca tcattgtaat cgtcataagt cactaacgat
     1381 tcataagcac gattcaattc ttggaatgct ttgacgtact cgatacggat ttcaagtggc
     1441 gtatgttcat caatggattc catggatgaa acctctttta acttaccata tgcttgcttg
     1501 aatctttttt tggattcctg ataacttgga taaatcaaac ccgattcttc ttttgcttct
     1561 gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tggctggctt tcggaaagta
     1621 acaattagtc cttttgtttt ttctggatac gtacggttcg tacgtgaaaa agcttggatc
     1681 aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg tgcgtcaaaa
     1741 cctgtcaata gacgatccac tacgatcact aaatcaattt gtttcccaaa ttgtttgaat
     1801 tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct
     1861 aatgaccagg aagtatcata atactgatta tattcctcaa taattttttg catttcttct
     1921 tgttggacac ttgaatctcg gctgttttct tctaatgaat aggtaatagc gattcgtgga
     1981 aaatcaggat cctccatcgt tcttccttca cgaatgggct gattaggaaa ctcttttgtt
     2041 aaccagtcag gttctttcgt catttctttg atcgctcgat aatacttttt ggccatgtca
     2101 atggaactcg tggttaagat cgcagacttc gttggacggc catttcgaaa atcaaatttt
     2161 gtataggcat tgtctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct
     2221 tcaaaaacag tagggtccaa ataactttct tttttgacag gctccatttg atcaatcata
     2281 cgattgatct gttccggtga atactctgca tatgtttcta cttcttttaa tttccgatag
     2341 attttattct ctaatgaagt cggctccacg gtgttttcat gctctacttg aaatcctaaa
     2401 accgagccat cttctaatgc attcttgatg gtataggtat gcaagacctc tccatactga
     2461 tcatgggtcg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg gaaaatcggt
     2521 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
     2581 tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg ccccattaat
     2641 ttttggaagc gattggttcc ttttttctct tcttgttttt ttgcatattt caacgccgca
     2701 tctagctttt gacgggttgt aataatcaca acatttgcat tggcatctgc caacagagtt
     2761 tcacttaact ctttggcact tcctgttccc acgatcaagc tattcgcttt ggcgttcccg
     2821 gaagaaatcc cagtattaaa ttccgaagca aatttcgtaa actctgtggt ggtttgatta
     2881 tctaaatctt tgcggtccac taacatgatc gtccgatcga tccccgattt tcgtgctaaa
     2941 agcttagtag atacaaaact agtcaacgtt tttcctgaac cagtcgcatg ccacacatat
     3001 cctgattgat gtttatttgc agcaataaac aaggcttgaa tcgcatgtac ttgataagga
     3061 tgtaacacca tgaggatttt attgtcttga tcttcactga caatcgtata atccgcaatc
     3121 aagcgatgag catccgggat atttagcact tgtttgcaaa actcatataa gttttctacc
     3181 tttttattat ctttcgttcg ccaactaaat aaaaacttag gatgcaaatc ctttggtaag
     3241 gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa
     3301 aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt aaacgcctga
     3361 taaaaactat cttttgcggt tacttgtttc agctcgattt ggacaatcgg taatccattg
     3421 atcagtaagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca
     3481 atttgatgga ccacttcata ggtagagatt cctccaccaa tgtcttgatt agaataaaga
     3541 atgagcgaga cagaacccag ttgcggatct tcccgttcaa tcgtgatccg agccattcca
     3601 ttttcacctt ttaaccattt cgcagcatca aagggggttt tcgttcgtag taacaattct
     3661 gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta
     3721 tttgagatga ttttttggcg taaattgacc caaagatctt cttctgattt tagatcggga
     3781 cgatacgtcc actgattatg cccttccccc aatacacgga tcaactgttc ttcgacttcc
     3841 gcttcctcac ggtgactaat tttcatttat cttccct
//
LOCUS       DAYYCW010000164         3861 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000164 DAYYCW010000000
VERSION     DAYYCW010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.169"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHAYA_002813"
     CDS             131..505
                     /locus_tag="ACHAYA_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8606546.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHAYA_002814"
     CDS             complement(619..1248)
                     /locus_tag="ACHAYA_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8606547.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHAYA_002815"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHAYA_002815"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ACHAYA_002816"
     tRNA            1668..1741
                     /locus_tag="ACHAYA_002816"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="ACHAYA_002817"
     CDS             complement(1879..2043)
                     /locus_tag="ACHAYA_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606548.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="ACHAYA_002818"
     CDS             2338..3666
                     /locus_tag="ACHAYA_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM8606549.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
BASE COUNT         1314 a          597 c          724 g         1226 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg g
//
LOCUS       DAYYCW010000165         3818 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000165 DAYYCW010000000
VERSION     DAYYCW010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.170"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACHAYA_002819"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACHAYA_002819"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM8606550.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="ACHAYA_002820"
     CDS             complement(2262..2513)
                     /locus_tag="ACHAYA_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM8606551.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="ACHAYA_002821"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="ACHAYA_002821"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM8606552.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="ACHAYA_002822"
     CDS             3463..3681
                     /locus_tag="ACHAYA_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606553.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1058 a          813 c          709 g         1238 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aaaatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaa
//
LOCUS       DAYYCW010000166         3812 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000166 DAYYCW010000000
VERSION     DAYYCW010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.171"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..307
                     /locus_tag="ACHAYA_002823"
     CDS             <1..307
                     /locus_tag="ACHAYA_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606554.1"
                     /translation="KFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGI
                     FLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(433..1110)
                     /gene="nth"
                     /locus_tag="ACHAYA_002824"
                     /pseudo
     CDS             complement(433..1110)
                     /gene="nth"
                     /locus_tag="ACHAYA_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(1117..1815)
                     /locus_tag="ACHAYA_002825"
     CDS             complement(1117..1815)
                     /locus_tag="ACHAYA_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8606555.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2083..3267
                     /locus_tag="ACHAYA_002826"
     CDS             2083..3267
                     /locus_tag="ACHAYA_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM8606556.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3273..3716)
                     /locus_tag="ACHAYA_002827"
     CDS             complement(3273..3716)
                     /locus_tag="ACHAYA_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8606557.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          990 a          754 c          632 g         1436 t
ORIGIN      
        1 aaaatttatg gaaagaagct ctcatgcaat gattctcact tttttatggt tgttgttcac
       61 cagaatctat atttttctga tgagcctgaa accgcccaaa tccattagtt tatgtttagt
      121 tataaatggt atttttttat tatcacttat catttttatt acacctttag atagatacgt
      181 cacttattta tataatcctt tggaatactg gacttatttt ataggtatat tggaaggcat
      241 tattttctac atcgggtatt ttcccaataa aaataacaac ttttattttt atcgcaataa
      301 attgtaataa gttaccatcc tagctactga gtatgtacat ttactttttc atgcaaaaaa
      361 gataaacgaa acagcttttc tccataataa caagataatt catacattta tttgcaatta
      421 aagaaaccac gtctacaggc gtggtttctt ttttagcgcc ttttctttta gacgcatccg
      481 atttttccca tcttgacata ttgatagcaa aggacatacc tcgcacttgg gatttcttgc
      541 cgtgcaatga tatcaaccaa aaaaaatcag tgtatgatgt gtctttaccc ataattcttg
      601 tggtactttc cgcatcagtg tttcttctac ttccatgacc gtagcatcta gtttgcagat
      661 cctcaggcgt tttgagacac gttccacgtg ggtatctact gcgattgctg gtatcccgaa
      721 tgcatccccc aatacgacat ttgctgtctt tctgcctaca ccaggaagag acatcaattc
      781 ttctcttgac gttggcactt gcccatcgaa acgttcaatc agctgctgtg cacacgcttt
      841 gatgtttttt gctttatttc gatacaaacc aatcgttttt attttaagaa tgatttcatc
      901 gattgaagct tctgctaacg catcgggtgt cggaaaagag gcaaacaaat ctggcgttgc
      961 tttattcact gatacatctg tcgcctgggc acttaagatc actgcaatca ataactcaaa
     1021 tggattattg tgcttgagtt ctccatgtgc ttcgggaaac attccataca tcgtttctag
     1081 agcttccatt gttttttgtt tgcttaacat attccgctac tcctccaacc aatttttcag
     1141 tgagactttc ggcaattctt cttgttgagc tgactgttct acttctcttt gaagaagctg
     1201 ctgttttctt cgtttttggt cttcttccac ttgttgtttt gtacgtagat tttttctttc
     1261 ccacgatagc aaaatgcggt caacgtaatt aaaactataa gcctgattta atacggcttc
     1321 ccgcagtgct agcttcaaaa tttccggctg ataatggtct tcttctagcc attgtccgat
     1381 ccgctggaac tcgatcgaag aaagcgggcg tccaaattct tgttcaaata attgatacac
     1441 tgactggatt tctttttctt gtgttttttc ttcgtatttt ttttcttgtg tttttaaata
     1501 ctcacctaat agatcataga tggggtaaag attatatcga tctgcttttt taccgttgac
     1561 aatcgtcgtt tctatcttga taaatccatt tgttacgaga cgatccagta tttgaaaaat
     1621 ctgatcttgt ttgattccca tatccatagc aatcatctgc aaatcaggga aagtatctcc
     1681 ttcaagctgt gccatatgca actgtagaac aaataaaaat tcttcagatt gtagaccgat
     1741 cctatgataa tttttaagta aaagattaga tatgactgtt tgtccagcgc ttaagtaatt
     1801 ttctaaattc aacatgattt ctcctccgct caatcacagt atacgtaaaa aaaacatggg
     1861 aagcaagaac aggtttcttt tctatttact tcactgatcg gtcagattta caatgtcgtc
     1921 tacttgattt ttgtttgttc atgtttttga tgcgtatctt cacttttcgg aataaataaa
     1981 ttaagtagat ttcgctgttc cttagaatgt tgacaaggaa acaaaattac tatacactat
     2041 ttttgtttcc aagttaacat tattgcataa aggagttttt acatgtacag tttcacaaat
     2101 aaacattcta aagtttttgg atacgtctct gtcttattca ctggattagg atttactatc
     2161 gtcagtcctg tacttccgtt tttaacgctg ccttattctc actccataca tcagcaagct
     2221 ttctatatca ctttgctcat gtctgtttat gctctagctg ccttcttatc tgcccccatt
     2281 ctcggtagct taagtgatca ttttgggcgt catccgatcc ttattatcag tttattagga
     2341 tcaagtatcg gatatttgct ttttggttta ggaaattcga tatggatgtt gttccttggt
     2401 cgtatcattg aaggattgac ggcaggagaa atctccacct tatatgctta ctttgccgac
     2461 atcacggaac ctaatgaaag aaccaaagtt tttggatgga tgggtgcttt agtcggtata
     2521 ggaacaacgc tgggtcctat tatagggggt ctattagctg agttggggaa ttctgttcct
     2581 atatttattg gagcactctt tactttctta aatgccgttt atggctacac atttatgcta
     2641 gaaagccttc caataaaaaa acgttcagta gatttaagtt tctcccatgt aagacctttt
     2701 caccaattac aacaattatt caagatttca tccgttattc ctttattgac agcaggtttt
     2761 gctgtttggt tggcagctgg ctctcttcaa tctatctttt ctcaattttc gattgacgcc
     2821 tttcagtgga aggctgggtt ggttggttta agcttctctt tgattggaat cttggatact
     2881 gtctcgcaat tgttcattat gcctcgattg ctcaaaaaat tttcagaaca gcagatcaca
     2941 agaataggga tgttcagtga aatcctcgcc tatctattca tcacattatc tggaattctt
     3001 ctactgccga ttttgttttt atttgggatt atctgttatg gtttcggtga ttctattttt
     3061 actcctgtat tcaatggcca actatccaat tctgtctcag ataaccagca aggtcttgta
     3121 atgggcggta cccaaagtat acaatctcta tcgcgcgtca tcggcccgct aatcgcagga
     3181 cagctttatg ctgcagcacc atttcttccg acggtattcg gatttttgct tatgattttt
     3241 gcctattatc actacaataa aaaatagtag cttcatttgc tacttcgtat aaattctctg
     3301 tatcgaccga taaaacgaaa tactgtgtca aattcttctt ttgacatatt ttcaaataca
     3361 gtagcatctc gctgtaccca tttttgatgg agttgctcgt gtctattcct tattttttgt
     3421 ccgattggcg tcaattgaaa atagatttct tttttattct cttcgctttg ataagtgttt
     3481 atgatccctc tagctatcag tttttttgtt agcttactga tggctcctct agtcatgtaa
     3541 ctagcattcg ctaattttgt cacgttagga tcagggattt tcccgatatg ttcaatcact
     3601 tctagttcat ttagagtaaa tcctgcaaat tctttttcca tttctggttt attacgatga
     3661 ataatctcat tagctaaatc cagatattct tctattagca gttgtctttt atccatttct
     3721 acaccttctt tttctttcat tatattgttt ttttgtttcc tattcaacaa agataaaaat
     3781 attttgtagg aaaacccaat ctgaaaccaa ga
//
LOCUS       DAYYCW010000167         3798 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000167 DAYYCW010000000
VERSION     DAYYCW010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.172"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACHAYA_002828"
     CDS             127..579
                     /locus_tag="ACHAYA_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606558.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACHAYA_002829"
     CDS             593..1282
                     /locus_tag="ACHAYA_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606559.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACHAYA_002830"
     CDS             1407..3758
                     /locus_tag="ACHAYA_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM8606560.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1286 a          499 c          779 g         1234 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaa
//
LOCUS       DAYYCW010000168         3795 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000168 DAYYCW010000000
VERSION     DAYYCW010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.173"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..1386
                     /locus_tag="ACHAYA_002831"
     CDS             37..1386
                     /locus_tag="ACHAYA_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8606561.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1514..2104)
                     /locus_tag="ACHAYA_002832"
     CDS             complement(1514..2104)
                     /locus_tag="ACHAYA_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8606562.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2251..3603
                     /gene="brnQ"
                     /locus_tag="ACHAYA_002833"
     CDS             2251..3603
                     /gene="brnQ"
                     /locus_tag="ACHAYA_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8606563.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1077 a          705 c          747 g         1266 t
ORIGIN      
        1 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
       61 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
      121 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
      181 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
      241 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
      301 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
      361 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
      421 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
      481 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
      541 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
      601 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
      661 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
      721 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
      781 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
      841 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
      901 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
      961 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
     1021 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
     1081 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
     1141 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
     1201 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
     1261 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
     1321 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
     1381 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
     1441 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
     1501 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
     1561 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
     1621 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
     1681 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
     1741 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
     1801 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
     1861 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
     1921 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
     1981 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
     2041 caagaactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
     2101 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
     2161 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
     2221 acttttaaaa aaaccgtaaa ggagttatgt atgacaaaaa aattatcatt taaagactat
     2281 ctttttatag gctccatgct tttcggtcta ttttttggtg ctggaaatct tatctttccc
     2341 gttcatttgg gtcaagaagc aggcgctgct accttttggg cgaatctagg tttcttggtt
     2401 accggtatcg gtcttccctt cttaggggtg attgccattg gtgtatcgaa aagctcaggt
     2461 gtatatgaat tagcaaaacg gataaatcaa acatatgcga tggtttttac catcttgctt
     2521 tacttagtta tcggaccgtt ttttgcttta cctcgccttg caaccacatc tttcgagatt
     2581 ggtttagcac catttatcga tcctagcaga cagacgctat atctagcaat tttcagtatt
     2641 gtattttttg tgctggcttg gtggttttca agaaaaccga caaagatatt agattatgtt
     2701 ggaaagttcc tgaatcctgc atttttggtt ttattaggtg ctttgcttct ccttgctttt
     2761 ttgaatccac taggttctgt aaatcaagcg cctattcagc caaattatca agagcatgct
     2821 tttttcacag ggtttactca aggatataac acattagatg cgcttgctgc tttagcattt
     2881 gggatcatca ttgtcacaac aatccaaaac atgggtgtaa caaaacctgc tgaaatcgct
     2941 aaagacacga tcaaatctgg tgcgatcagc attatattga tgggaatcat ttatactttg
     3001 ctcgcttatt taggtgctat gagcttaggt agctttgctc taagtgaaaa tggcgggatc
     3061 acgctggctc agattgctga tcattattta ggtacttatg gcagtatctt attagcattt
     3121 atcgttatat tagcttgctt gaagaccggt attggtttga ttacagcttt tgcagaaaca
     3181 ttcacagatt tgtttccgca aagaaattac gcattctttg ttgcacttgc tagtatcctt
     3241 ccttgtttag cggcaaacgt aggcttgacg aatatcattc aattttcgtt gcctgttttg
     3301 atgttcatct atccattagc aatgacactg attttactag tgttagttgg tcctttgttt
     3361 aagcagcgta cggctgttta tcgaatgact acttatttta cattgattgc ttcgattttt
     3421 gatgggttga acgcttgccc agaaactatc aaacaaacac ctattgttca aaatctttta
     3481 tatgttgcgg aaagctactt gccgtttttc aaattgggga tggggtggat cgtaccagca
     3541 gttatcggtt ttgttatcgg actgatttgg agtttcgcta agaaagaaga agtagcagat
     3601 tgataaaaga ggttgtgaaa gagacgttca gccttgagca ataaaaagta attttgaaaa
     3661 atggcttctc acatttttgc ataaatttga cttaataccg agaaactgac ttttgaacac
     3721 cgtttattga gaggtcgtga aaaagccgct tagtttcaag tattaagaaa tattttgtga
     3781 aaatggcttc tcaca
//
LOCUS       DAYYCW010000169         3792 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000169 DAYYCW010000000
VERSION     DAYYCW010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.174"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            211..891
                     /locus_tag="ACHAYA_002834"
                     /pseudo
     CDS             211..891
                     /locus_tag="ACHAYA_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            1031..1570
                     /locus_tag="ACHAYA_002835"
     CDS             1031..1570
                     /locus_tag="ACHAYA_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606564.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            2590..3759
                     /locus_tag="ACHAYA_002836"
     CDS             2590..3759
                     /locus_tag="ACHAYA_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8606565.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT         1390 a          538 c          651 g         1213 t
ORIGIN      
        1 gaaataggac ataaatatat gagtatgaga aaggtgattc catgtttaat aaatgggcaa
       61 accacccaat gaactcgaga ggtagagata atttgaagga gacggacaag ttctgggaag
      121 aagtatatag taaaaacggt tctgttgcaa agttttccaa aaaatctatt ttagtgtaaa
      181 attgagaaaa aagacagaga ggacagagta atgaatcatt ttaaaggcaa acaattcaaa
      241 aaagacgtca ttattgtcgc tgttggttac tacctgcgtt acaatctaag ctatcgtgaa
      301 gttcaggaat tgttatatga tcgtggaata aatgtttgtc atactacgat ttatcgttgg
      361 gtataagagt acagcaaagt cctctattat ctttggaaga agaaaaatag acaatccttc
      421 tattcatgga aaatggacga aacctatatc aaaattaagg gacgttggca ttatctttat
      481 cgtgcaattg atgcggacgg cttaacctta gatatctggt tacgaaagaa acgggatacg
      541 caagcagcct atgctttctt aaaacgactc cataaacagt ttggtgagcc gaaagcaatt
      601 gtgaccgata aagcaccttc tcttggctcc gcctttagaa agttacagag tgtgggttta
      661 tatactaaga cagagcaccg aactgtgaag tatcttaaca atttaataga acaagaccat
      721 cgacctatta aacgacgaaa taaattttat caaagtctcc gtacagcctc ttccacgatt
      781 aagggcatgg agaccattcg aggaatatat aaaaagaacc gaagaaatgg aacgctcttc
      841 ggcttttcgg tgtctactga aatcaaggta ttaatgggaa taacagccta agatatttgg
      901 agttcagaga gggcgcgttt gattttcaaa cttcgcaaca gaaccgagaa ccttgggttt
      961 tcaagtgctt tttggttcat taatcttaac catagtgttt ttaaagtgga aaacaagcaa
     1021 caaaaaaagt atgtggagcc ttgtaggaat agttcttatt atttttactg tgactgcagc
     1081 aacagaagca aattattcgc ataaaattca gtcgtataaa ggtcgtttag ttatgaccaa
     1141 aaaagatgtt acacgttata catcctcaag aactggaggc ctatatgact atgcaccatt
     1201 agatgcattg aaatataaag atcatttaaa gaaacatgaa gtaaaagttg gtgatcggtg
     1261 ggagaatgca tctcataaag catataattc caaaatagtc tatacagtag aagatgccga
     1321 tgagcaaaat atcacattac ctgtattcaa ttattgggga acagttgtga aagtaaatgg
     1381 gaaaaagagc actatagaaa atgacaatgg tttagtgtct attaaaggta cggggaaaag
     1441 aactatagtt gaggtttcat ctacgtatcc aactacgatg attctatctt tattaattag
     1501 ttcaataact tttatattct tatttacacg ttatttaata agatgctgcg gtgaaatgaa
     1561 aatagattag atagatatta taaatatgtt gattctattt tcatagcaat ggagaaaata
     1621 cattagcaat atataaaaga aggtatcaag agtcacagtg aatttattcc gaaaagtgaa
     1681 aagagcaaat ataaaattta atgaaaatat agaactaaat aggaaaacgt agacgtaatt
     1741 ttctacgttt tccttttcat atatattaaa tattacggaa aattatataa actaatttag
     1801 tgttttgttt gtaattccta attttgctcc acttttagat aatttttagg tctatttaac
     1861 tcatcagaca ttctatgctc atttttctcc aaatttgttt caactctttc gtacttaatt
     1921 ttgcgtattt gaaaattctt cttttagagt acttttatta cgatactagt atgatatata
     1981 tctatagcaa acagttaaaa tcttcctaaa ataagcgaat agcattccta aaacctataa
     2041 aatttttaga tgaagcgaca agtggacaat taataaacta cttgaacatg aaattatgga
     2101 tcacctttca aatttagaaa atggaattct tatacttatt gcgcaacatt tattgattgc
     2161 aaaattatgt gacaaaaaaa actagtagaa gaaagaataa tggcttgata ataaagtact
     2221 acctattaaa ggtagtactt tatttaattt agtataaatt aacatactac tatattttag
     2281 gtttcatatt atataaatta ataatatact accatttgtt ggtagtataa tttaaaggta
     2341 ataagttatt cttttattac tatgtttggg ttttatagca aagaaagttt aataagagtt
     2401 tggacctagc aaacaatatt aatgaggtca ttaaatctta ttatctttag tgatataacg
     2461 aaatatctac acagacaaaa tgatagcgta aaatattttg tgtaaataga tatttagggt
     2521 agaaaaaaag aaagtccatt ctgtaaaatt aaagttacca caaccgaaat tttataggag
     2581 gactttctca tgcccaattt tactacagaa attatggaaa cactaatcaa taaaggtgat
     2641 ttagatgaat tatttcgatg tcatttagaa ttagctgtta attcattatt gcaggctgaa
     2701 ttaacagctt ttcttgatta tgaaaaatat gatcgtgctg ggttcaattc aggtaattcc
     2761 cgtaatggga attattcacg ttcattcaga acagagtatg gagaattaaa tttggtaatt
     2821 cctagagata gaaatggtaa gttttctcaa caaactttac ctgcttataa aagaaccaat
     2881 gactctttag aaaccactat tattcagcta tttcaaaaag gaattactat gtctgaaatc
     2941 tctgaattga ttgaaaaaat gtacgggcat cactatacgc cacaaaccat ttcaaatatg
     3001 actaaaatag tttctgaaga tattattgcc tttaaagaaa ggtccttaga atccagatac
     3061 tctgtcattt ttatggatgc tactcacatt cctttaaaaa gacaaactgt ttcaaaagaa
     3121 gcagtttata ttgtaattgg cattcgatta gatggaacca aagaggttct tgggtttagt
     3181 cttgctccaa ctgaatctgc ttatgtttgg aaagagattc ttcaagacct aaaagaccgt
     3241 ggtttaaaag aggttttatt agtcgtaact gatggtttaa gtggcattaa cgatagtatt
     3301 catagtgttt atccaaatgc tcaatttcaa caatgttgtg tccatatatc tagaaatatc
     3361 gctcataagg ttcgtgttag tgatcgacaa gaaatctgta gcgatttcaa attagtttat
     3421 caagcaagtt caaaagaaga agcaaataat caaataaggt ttatgataga taaatggaaa
     3481 aagcagtatc cacgagtggt taaattgctc atgaatcctg ctatattaac cttttataat
     3541 ttccctccgt caatcagaag aactatttat tcaactaatt tgattgaagg ttttaacaaa
     3601 caattaaaga aatataccaa aagaaaagag caatttccta atgaagaatc tctggagaga
     3661 tttctagtat ctcaattcaa caactataat caaaagtttc tatgccgagt tcataaaggt
     3721 tttaaagaaa tacatgatac attagaatca atgttttaac ttgatcaaca gaatgggctt
     3781 ttccatttac ac
//
LOCUS       DAYYCW010000170         3717 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000170 DAYYCW010000000
VERSION     DAYYCW010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.175"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..155
                     /locus_tag="ACHAYA_002837"
     CDS             72..155
                     /locus_tag="ACHAYA_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM8606566.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            280..1017
                     /gene="erm(B)"
                     /locus_tag="ACHAYA_002838"
     CDS             280..1017
                     /gene="erm(B)"
                     /locus_tag="ACHAYA_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8606567.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1280..2143)
                     /locus_tag="ACHAYA_002839"
     CDS             complement(1280..2143)
                     /locus_tag="ACHAYA_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8606568.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2145..2417)
                     /locus_tag="ACHAYA_002840"
     CDS             complement(2145..2417)
                     /locus_tag="ACHAYA_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8606569.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2434..2643)
                     /locus_tag="ACHAYA_002841"
     CDS             complement(2434..2643)
                     /locus_tag="ACHAYA_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8606570.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2721..3617)
                     /locus_tag="ACHAYA_002842"
     CDS             complement(2721..3617)
                     /locus_tag="ACHAYA_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8606571.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1143 a          652 c          575 g         1347 t
ORIGIN      
        1 aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg
       61 agggattcgt catgttggta ttccaaatgc gtaatgtaga taaaacatct accgttttga
      121 aacagactaa aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt
      181 gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct
      241 gtatttattt atagatgtaa tcacttcagg agtgattaca tgaacaaaaa tataaaatat
      301 tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta
      361 aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg
      421 gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg
      481 tcagaaaaat taaaactgaa cactcgtgtc actttaattc accaagatat tctacagttt
      541 caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca
      601 caaattatta aaaaagtggt ttttgaaagc catgcgtctg acatctatct gattgttgaa
      661 gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact
      721 caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa
      781 gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg
      841 aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact
      901 aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccgttact
      961 tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc
     1021 tatgagtcgc ttttgtaaat ttggaaagtt acacgttact aaagggaatg tagataaatt
     1081 attaggtata gggcacctct aataactacc aattaattca cctcaaaatg aaattagaaa
     1141 aaaacagcga aatttttaaa tctatttctt atcgatacaa attcctcgta ggcgctcggg
     1201 accccaacat gaggaaatgt ttttataaaa aaagagtagt taccgttttt tggtaactac
     1261 tcttttcctt ttttaaggtt taaatacctg gaagtttagg tgttttgggt attggtggtg
     1321 taggtagctg caagctttcc attttttgtt gaattgcttt aaattcaggg gtttcttggt
     1381 gttgattttg aaccattttt tgctctattc tctccaaagt cggttgaatt tctttccctg
     1441 atactttacg atttaattct ctttctaagg tctctttcgg actaatggaa ggcgtttcta
     1501 agcttgaata gagtttcact ccttctctat tgtaaagcct tatatcgcta aataagcccg
     1561 ttttatgaag ggtttctaaa ttggtcggta agtttttgac aacaatatca tgcgcttgtt
     1621 ttggtgttgc cctagctgtc attggatcat ctgcatacat ggtttcatat cgttcaatcg
     1681 ttcctaagta tgattcaatt ttcggtactg ccatgacgta tatttttgtt tcataacctt
     1741 tagattgaag cattgtggct gtttgaatgg gaacgtctgt tgttcgtcct gttccttcaa
     1801 tcactaaatt atagccttga tcgcttaaac ggctaataag tgcttctgtc atgcgattcg
     1861 aataaggggt aacgtgtttt actacgtctt tttcatagag tttcactaat tcgtcaaaat
     1921 ttgggtgctg ttgtttaaag gtatcattat caatgacaat aacattccct tgtgtttctt
     1981 caaaaattgc tgatcgcaaa ctggttttcc ctgaccctgg ttgcccacca agtaaaaaag
     2041 cggttggcga ttcaaccgct agtctatttt tagttagtcg ttcaacattc ttttctaaac
     2101 gatcttcaaa ttgcttttcg gtgaagtcag taatattcgc catgttaagc cactttctct
     2161 ttattcaaaa tttgttttga atattgattc attgaccgcc aatactcatg aatagcttgt
     2221 aattcttcaa atggttgttg aaataaatca acttcttgtg ccacttctaa ttgatttaaa
     2281 agattcactt ctagtaaacg agtattttta gtatctagtt catgattcaa aacataattt
     2341 aatacccgat tataaacact tgccgataac tcattaatga tttcaatttc aaatgttttt
     2401 tcatacgtaa ctgccatttc attttcactc cttttaaagt ttgtcaggta aatatttccg
     2461 tatatattct tctaaggctt gatccataac ttcttttacg tttccgccat tctttgctgt
     2521 ttcgattttt atgatatggt gcaagtcggc acgaacacga accgtcttat cccccattat
     2581 atcttttttt gcgctgattg gcgtatcatt tcgttttgct ttctgtgcgc ctaaatttcc
     2641 cacgatcgct cacttctttc tatttcttct tactatttat tttatcatca cctatcacaa
     2701 atcacaagtg atttgtgatt ctattctttt tcattttcta attgaatgat tcgctcaatc
     2761 atttcaaaaa atacgttctc atacatagac aaaacttttt tgtcatagcc tttgtgttct
     2821 gtaatgccat ttttagacca agtacttact ttattacttc gcttgataat atttcggaaa
     2881 accaaattat cctctttatg ttctttgtac agttcttcca ggtttgattt tatcgttgcg
     2941 ctgtccgtat caactaaata aggaacaaaa ccaatcatat ctagtccagg gttaaactgt
     3001 tcttgcaaat caatcaaata ggaaatatag ttttgaatgt tgtttgtact ttcttcttct
     3061 gcttgtaaag ggatcataac gtaatcactc gccacgattg cattatttgt ataaacgctt
     3121 ggcgttggta cagtatcaat aataataaga tcatagtcac tttttaaagg tgctaaaaga
     3181 gtagcaagca atctactttc attttcaaac gtccatgagc gagttaattt tggcagtaac
     3241 atcaaatcaa acgtgccagg gatcaagtct aaattatcag tcaaatgaat aatagaagaa
     3301 gccaagtttc catttttcaa gccttcataa aaattgacac gtggcaattc tacctcaaaa
     3361 gtttttgcta agtcttttgt caatgttgcc tgtaagtcct tatcgatcat taaaactttt
     3421 aaattcaatt tgtctgtcaa gtaagcaaac atagtcgata atttggactt tccaacacca
     3481 cctttaaagt aattattcaa gataataatt gcttcatttt tattgtttaa aatacgtctt
     3541 aattcttcaa gtatttttag tttttccttc tccataacta cgccccattc tttttttgta
     3601 tagtaatatt gtatcattaa gaatcacaaa tcacaagtga tttgtgatta atcacttgtg
     3661 atttgtgata agtgatttgt gattaatata taaaagccct ctttaaaggg cttttat
//
LOCUS       DAYYCW010000171         3712 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000171 DAYYCW010000000
VERSION     DAYYCW010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.176"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..353)
                     /locus_tag="ACHAYA_002843"
     CDS             complement(<1..353)
                     /locus_tag="ACHAYA_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606572.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRT"
     gene            complement(613..1161)
                     /locus_tag="ACHAYA_002844"
     CDS             complement(613..1161)
                     /locus_tag="ACHAYA_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606573.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="ACHAYA_002845"
     CDS             complement(1162..2019)
                     /locus_tag="ACHAYA_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8606574.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="ACHAYA_002846"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="ACHAYA_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="ACHAYA_002847"
     CDS             complement(2581..2910)
                     /locus_tag="ACHAYA_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8606575.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="ACHAYA_002848"
     CDS             complement(2987..3259)
                     /locus_tag="ACHAYA_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8606576.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="ACHAYA_002849"
     CDS             complement(3259..3522)
                     /locus_tag="ACHAYA_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8606577.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1089 a          752 c          472 g         1399 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatacccgt ggtgctagtt
     3661 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aa
//
LOCUS       DAYYCW010000172         3674 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000172 DAYYCW010000000
VERSION     DAYYCW010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.177"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..277)
                     /locus_tag="ACHAYA_002850"
     CDS             complement(56..277)
                     /locus_tag="ACHAYA_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606578.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(375..620)
                     /locus_tag="ACHAYA_002851"
     CDS             complement(375..620)
                     /locus_tag="ACHAYA_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606579.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            860..1474
                     /locus_tag="ACHAYA_002852"
                     /pseudo
     CDS             860..1474
                     /locus_tag="ACHAYA_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(1844..2845)
                     /locus_tag="ACHAYA_002853"
     CDS             complement(1844..2845)
                     /locus_tag="ACHAYA_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM8606580.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3004..3270)
                     /locus_tag="ACHAYA_002854"
     CDS             complement(3004..3270)
                     /locus_tag="ACHAYA_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8606581.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3260..3616)
                     /locus_tag="ACHAYA_002855"
     CDS             complement(3260..3616)
                     /locus_tag="ACHAYA_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8606582.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1170 a          661 c          493 g         1350 t
ORIGIN      
        1 agctgtattt tacatatagt aaccaaagag ttaataaaat aaaaagagcg aaccattata
       61 gatcagctct tttttcattg ctgtttttaa tcttatctag aatcacttca aatgtttcaa
      121 aggtatttac atctaactga cataaccaaa gcaagtactt cttttctttg taagtccaat
      181 caatattacg gtaagaggac attagattat ggtaccttgc tctttgttca actgaagcca
      241 attcccagcg tttttcaaat attttttctt cttccattat tgatccctcg caagacgcta
      301 cctcaaactt aaataatggg cagcgaaagt ctaattcctt agatctaagt atagcaaaca
      361 aatatgaaaa aaacttagaa ggtagtaaaa gttcttccgt cttttgcttt gcttctattc
      421 ttaatgtgtt tcgttatcgg tgccctacaa agattttcat taattacaca ctcatttatt
      481 gttgtgtatt ttctgacatt tctctcttga tcaattattt taattccatg ggcatcctga
      541 tagttcatag gagaggagcg gctatcagaa atagttgcac ctatctcttg aacttctttt
      601 acaacttctg gaaaatccat ccatcctgct tcagctgtca aaagtaataa tctataatat
      661 ttatctttcc ttttcaatct aagaaatcgt caccccattc tttttcttct attataaact
      721 atttaacttt tagaattcaa aaaccgtttg tctatttaga gtttacccta aatagacaaa
      781 aatataacat tgaaaaattt agagttctaa cgtatgatta gggtgtatta taaacgttat
      841 agaaaagtag ggaataaatt tgaaaattgc ttatattaga gtttcatcca tcgatcaaaa
      901 tgaacaacgt caaattgaag aaatgaaaaa gtttggcgct gaaaaaattt ttatcgaaaa
      961 acaatctgga gcgacgatta gtcatcggcc gattttccag gaagctttag attttgtcag
     1021 agagcaagat atttttattg tagaagccat tgatcgactt ggacgcaatt atgacgagat
     1081 tatcgattca gtgaattatc taaagaagaa gaatgtgcga cttattatca ccagtttacc
     1141 gatcatggct gaagcaattg gaaatcctct cctagataaa ttcatcaaag acttaatcat
     1201 ttaaatctca gccatgattg ctgaacaaga acgtactgaa tcgaaacgac gccaggcaca
     1261 aggaattaaa atcgctaaag caaacggcgt ctataaaggt cgtcctaagt tatacagtgc
     1321 agacaccaaa gatcctcaac gtcgccttgt ctataggagt atcgttcagg atcttgagaa
     1381 tggggttgct atctcaaaga ttgcaacaga ctacaatgtg actaggcaaa caatttaccg
     1441 aataaaaaaa gaaatcgacc aactaattgt atgatttctt ttttgaatca cgtaattatt
     1501 aattagcttt gtcatcaata cagacaatca ttttagattc atctagctaa gttgattaca
     1561 tttatgttaa aaagaattta gttaacataa atataattga tgcataaatc gtccttattt
     1621 aaaggcattt aacattgaca acttagtata atgtatctta tgtaaactaa taagatttga
     1681 attttatttt atacacactc gataatagat caattttatg gtcccccgca tttacgattg
     1741 cacaagagaa acataaagtc gacctattag catttctttt caattcaaac attaaaaaag
     1801 tttatacctc tgctactaaa aaatgaaatt atctatttaa aatttaaaac ggtaaatctg
     1861 attcatccaa attggaaaat ggatcagcct gtattggagc tttgtatttc tccataataa
     1921 cagatatctt tttttcattt tttaaaaaat accttttaaa tttcttatag tcctctgaaa
     1981 tcaaaatata gcgtgatgga tactcatcaa aagaagaatc gtattcttcc tttaaaattc
     2041 ccgatttact taatttttct agtacatcaa atactctatc actcaagtaa ctccttaaat
     2101 tgttatcact ttcccattga actacttttc tataagttga attatcagtt gaaaagatag
     2161 aattttcatc cataaacgca tatattagca gcaatctttc atcattgtca aattctattg
     2221 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
     2281 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
     2341 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
     2401 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
     2461 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
     2521 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
     2581 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
     2641 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
     2701 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
     2761 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
     2821 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
     2881 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
     2941 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
     3001 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
     3061 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
     3121 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
     3181 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     3241 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     3301 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     3361 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     3421 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     3481 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     3541 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     3601 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     3661 taaaaattcg aatt
//
LOCUS       DAYYCW010000173         3594 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000173 DAYYCW010000000
VERSION     DAYYCW010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.179"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..286)
                     /locus_tag="ACHAYA_002856"
     CDS             complement(<1..286)
                     /locus_tag="ACHAYA_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606583.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIG"
     gene            complement(584..1558)
                     /gene="sgrA"
                     /locus_tag="ACHAYA_002857"
     CDS             complement(584..1558)
                     /gene="sgrA"
                     /locus_tag="ACHAYA_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM8606584.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1754..2593)
                     /locus_tag="ACHAYA_002858"
     CDS             complement(1754..2593)
                     /locus_tag="ACHAYA_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8606585.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2778..>3594
                     /locus_tag="ACHAYA_002859"
     CDS             2778..>3594
                     /locus_tag="ACHAYA_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM8606586.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDK"
BASE COUNT         1199 a          741 c          528 g         1126 t
ORIGIN      
        1 aaccaatcgt tccctctaaa agtatagaat aaactttact cagtaaaaac tgagaagaaa
       61 actcgacttt tatcatcttg ttttctgaaa gaaaagataa tttatattct ggataccttt
      121 cgcaaaacga ttctgcttgt tgaatcaccg aattcatctt agccagggtg tagtttagat
      181 ctaaagcgat atccttcacc acatataatt tctcgtctct ttcataacaa tagttcaaaa
      241 attgatatac ttgcctttca ttgatatcaa taaattcttc aaacaaagta catactcctt
      301 tttatctatt atttagactt attccgacag ctacatgaat aacgtacaaa tagattcgtt
      361 actgattttt cagagatact accatgcgca gtgtgcattg tcaattattc ttgctcttat
      421 tttccatttg tgttcctgtt atttcctgtt tctcaaatat gttagtaaac aaaaatagat
      481 ctggcaaagc tacaacttcg ccaggcaata cagcactctc gaatgaaata ttcactcaaa
      541 aatctatcgt tttattcaat tttagatctg ttgcttttat tctttatttc actcgatgga
      601 agagagaacg aaaatagctt gctcctacaa tcaaaatgag taacacaccc atccatgtaa
      661 ttagcttgtt caaggttcta ctaccagttt ctggtaaatc gctttttcta ttatctgttt
      721 ctttcgcttt ttcttgatga tcaattccac gagaaacaaa atcaggctgt tttagcacac
      781 tttttaggtt gctccctgca tttctctttt gttccttagt tggtatgata ggtaccaaat
      841 caagttcatt atttactttt cctgtgctgc tttcgctgct cgtgcttcct gtgctactct
      901 cactactcat gcttcctgtg ctactttcac tactcgtact ctccgtactg ctttcgctgc
      961 tcgtgcttcc tgtgctactt tcactactcg tactctccgt actgctttcg ctgctcgtgt
     1021 tttctgtgct aatttcactg ctcgtactct ctgtactgct ttcaccgctc gtgttttctg
     1081 tactgctttc actacttgtg ctatccgtac tgcttggcgg tccaacaact gtccaactgc
     1141 ttggtttcaa attctccgtg atcgtcatct gtccatcata aaaaacaatc aaatcaactt
     1201 tatctttact cttgatctca tcaatcgata gttctgcaga aatcattatt gtatactcaa
     1261 taccagcggt ccattgtacg tcttttaaat aaacttcccc aataactttt tctgaacttc
     1321 cattaccatc ccaaccatat gttagaacag catgtacatc actagcattc acgcttttgt
     1381 ttggtttgac tggaatagta atgacttgtt gtgtcatgtc ataaacaatc cgatcagttt
     1441 tcacagtaaa tgaaagattc tcatttgccc gttcatttgc agaagtactg attccaatca
     1501 ttgctgcaat tatactcagc ccaagaataa caaataatac ggtcgctgtt ttcttcattt
     1561 ctatcactct ccatataatt accttctatt tgataaagga attatataaa atgaaaatga
     1621 tgagaacacg acagtcagtt gtgaaaatct aacaaaagat gtcgtgtatt cgcttgaatt
     1681 tgtttacttt ttcgtttaat caatgacaga cttaaaaaag caagaccgat atcagccttg
     1741 ctttcttcat cttttattga taaacagcag tgtatttctt catccgcttt gttttatatg
     1801 catagagtaa caaaccaaca atcaagacta acaagaataa tgtagttgat atagatgctt
     1861 cagccccaaa gtttccccca gtaattattg tattttgcga ttggatcaga gtggagaaaa
     1921 aaccttgttc ttgcgttccg ctgactggta tacccaaaat agctccttgt gtccaattcc
     1981 aagtaatatg agcaccaata gccgtaaata aacttcctgt actgtagata agtgcgctga
     2041 aaaagcaacc tgctaaaaag atattgaaaa ctgccagaaa tgtcatgtta ggattcaatc
     2101 cgtgcaaaag agcaaataat gcagcagatc caatcattgc tattaacgga ccaaatctaa
     2161 gcatctgatg ctgaaaaaag ccacggaata aaagctcttc tgatgccccc actgcaataa
     2221 ataaaataaa actcgttatc agcggcacaa tgtttgcgtt tcctgaatct aaccgatacg
     2281 cccccgtcaa atataatgga ctagtaataa gcagaaacac tccaattcca acaacgagac
     2341 ctcccgctaa atacccccag tcttttcctc gaacagaaac accataatct tctattcttt
     2401 ttccttcgac cttactagca agaaataaag ctagtaaaaa aacagctaaa tgtccgaccg
     2461 taccggcaat tgctccaatc cattcactat ttccaataaa catatccgca agtgcattat
     2521 tgacaagaat tcctaaaaca aaaccaacaa agactattaa aacagatcca atcaaccgaa
     2581 taatcctgat catctttcat ccctccttta tattaagttt aacaaccctt ttctttcttg
     2641 tctcgtgaaa actatgtaaa tgttctccat catagagaga atagattttt acgaactaag
     2701 aatatctaca gcaaccttac gaaaatgaaa gactgtctcc ctaattcttc tgttagactg
     2761 tttttaataa ggaaaggatg ggaaattttt tgaaaaaaca actactaatt atgtgccttt
     2821 cagcatgtgc aattttacta ttctcaggtt gtagcaagtc caatgaaacg attgcaacgt
     2881 ttaaaggtgg aacacttaca gttcaggatt tttatgataa cccaaaagtt aaatcaaata
     2941 atcaaacact attaaaaaaa atgattgtct tcaaagcatt tgatgatgta tacggaaaag
     3001 agatttcttc aaaggaagta acaaaagaat ataatgaaca aatcaaaaaa ctcggtccaa
     3061 actacaagga acaattaaaa gcagttggcc aaacagaaga gacatataag aaactcttta
     3121 aacagatgct ggctttccaa tacggtttga aagccaatgt aaaattaaca gataaagatc
     3181 tagacactgc ttggaaagaa ttctatccag aagttagtac acagatcatt ctattttcga
     3241 ctaaagaaga agcagataaa gcaaaaaaag aagcaaatga aggagaaaat ttctccaagc
     3301 ttgttcaagc ttatggaaaa aataaaacat taaaggaaac tgatggaaaa atgaattttg
     3361 attcaaccaa tccagagatt cctactgaag taaaaaaagc agcatttaaa ctaaaaaacg
     3421 gggaagtttc tgatatcatt cctgtaacag atccaacgac ttatcagcaa agctattatc
     3481 tagtaaaaat ggtaaaaaaa caggataaag gttctaataa agacaaatac aaatctgagc
     3541 tagaaaaaat tgctaccgaa gcaaaaactc ttgatacgga attcatggat aaaa
//
LOCUS       DAYYCW010000174         3539 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000174 DAYYCW010000000
VERSION     DAYYCW010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.180"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..488)
                     /locus_tag="ACHAYA_002860"
     CDS             complement(<1..488)
                     /locus_tag="ACHAYA_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8606587.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     KE"
     gene            complement(478..1677)
                     /locus_tag="ACHAYA_002861"
     CDS             complement(478..1677)
                     /locus_tag="ACHAYA_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8606588.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1807..3183)
                     /locus_tag="ACHAYA_002862"
     CDS             complement(1807..3183)
                     /locus_tag="ACHAYA_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8606589.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(3183..>3539)
                     /locus_tag="ACHAYA_002863"
     CDS             complement(3183..>3539)
                     /locus_tag="ACHAYA_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8606590.1"
                     /translation="IRLVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARAL
                     VSDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILEL
                     SGKKLKKVNKMNLEVE"
BASE COUNT         1085 a          678 c          519 g         1257 t
ORIGIN      
        1 atttctttcg taaacttatg tttattggcg ttagctttga tgtgggtaga atccagatac
       61 aaattatcct ctgttacaaa acctgcttta actgcttgtt ctaaaatata ggcaaaaata
      121 tcctcaaaca ccgtcgtttc tctaaatcgg cgaacataat tctttccaaa agttgagaaa
      181 tgggggattt tctcctcgaa ggagtagcct aagaaccagc ggtaagcgac attggtatcg
      241 acttccttaa tggtttgtcg catggaacgg ataccgaata aatactgaat aaacaccaat
      301 ttgatcagaa tcactggatc gatacttggg cgacctaaat cggagtaagt ggcttcgacg
      361 attggataaa taaattcaaa atcaagcgcg gcatcaatct ttcgaacgag atgatcttga
      421 ggaactagat catcaatcgt aagcatcgca aattgatttc taccattcgt attgttttta
      481 gacatcatag ctatcactcc ttgcattcgt tttcataaat ctattataaa agaaaagact
      541 gtagacatca tccataaaat ggactttgtc tacagtctgc gcattctcat tagagaacac
      601 tccctttttt gtgacttttc tgaagctaga gtgaatactt tcaactgcat tagtagtgta
      661 catgattttt ctaatggcgc tgccataatc aaagagttgt tcaacatgag aaaagtttcg
      721 tttgcataca tctactgcac cagaataatg agaccaccga ttttgaaaac tgtcgaaggc
      781 agcgtgtgca gcgtttaaag aagaagcact gtagaatttt ttcatgtctc gacagacttc
      841 cttataatcc ttacttggga ggtatcgtaa cgcatttcga acaagatgaa caatacagcg
      901 ctgcacaatg acagaaggaa aaattgcttt tgcgccttct ttgagaccgg aaacaccgtc
      961 catcgaaatg aaaaaaacat cttctacacc acgtactttc agttcatcaa atacttgcat
     1021 ccagcgattt ttagattctg tttgattgag ccataggcct aaaatctcct tgtttccttt
     1081 aagatcatag ccaagaatgg tgtacacagc atattcttta gcttcatagt tttcgcgtaa
     1141 agtgacgtac atacaatcaa cgaatagaaa tgcataacac ttggctaatg gccgagcttg
     1201 ccattcttcc aattcaggaa ggacggcatc cgtgatatca gaaatcattt cgtgggaaat
     1261 atcaaagccg taaatatctt caacggttgc ggcgatatcg cattgactca ttcctcgtgc
     1321 atacatggaa agaaccttac cttcgatgtc ggagacatct cgttttctat taggaatcaa
     1381 ctcaggttca aaagaagcct cccggtctct gggaacatca atgtttactt taccaaagct
     1441 cgttttaagc gtcttattgc cataaccgtt tcgtcgattg tcatgtttct ttggctcttt
     1501 agaatgggcg tcatacccta aatggttatt caattctcct tgaagcattt tttcaaataa
     1561 gggaccaaac acatctttta aaacatcttg catatcctct acagattcgg gttgataagt
     1621 attcaaaatg gattcagcta acttctcagc atcaggattt cttttctttc tagccatcgt
     1681 gtaatcacct cttgatctta ttttagaaaa ataaaaaccg tgaagcaacc acccgcctag
     1741 gattgatggt tacttacacg gtttatatta cactctcatt tttggtgcta aaaagcaccg
     1801 aaaattttaa ttattgtcaa ttaataattc ttttggattc cttcttaata tattgaatga
     1861 agaaataatc aatgatacaa gaagtactaa actcataaat ataactacat aaatcatgaa
     1921 tttaggtaat acattgatat ctaaacttga taatgtcttg ttaaatcctt cagcttctgc
     1981 cccaccacct aattggctag atgcagattg tttagctatt tgtttagcta tatcaccagt
     2041 aaccttattc aatatattat tcccaagctt gtcagctgta tattgagcta agaaatagga
     2101 accaacgaat gcggggattg atataaatac catctcaatt aggaattgac caaaaatttc
     2161 taattttgat atacctaatg atagtaatac tgctatttct ttctttctag cattcatcca
     2221 taagaataat aataatgaaa ctacaactcc tgcaaatatt aatgagccaa aaaataattt
     2281 atttgaaatt gagtaaatac ctgatataga ttgttgtaat gcagggtaat ttgatgaact
     2341 tttaatcaaa ttatattctc tccaatttat atctaatttc ccaagatctt ttattacact
     2401 atcaagattc ttatcacctt ttacaaagaa tgttgcatct tggtatacag ctgtatcttc
     2461 ggtattacca taaactttag cagctgtatg aatatcagtg attaatgtat tttcgtatag
     2521 ttcttgtgct gcacttactc caccgctatt atgcccatcg aatagacctt taatttctac
     2581 ttctactgtt tcatctgcac ctttctcatt atcagcatca aataaattag attttatttt
     2641 tatcttatct ccaactttaa ggttattttt ttcagctaaa tctttatgca ttaagatttt
     2701 atttttatct tcattttcta aatgctttcc ttctactaat ttatatgctt ctgatacaaa
     2761 ttttgtttct tttgttgagt cgttaacccc agttaacata actgttcttt taaaattctt
     2821 tgctctttca ggtgattgat tcgcaagagt ctcttgagtt tcaataatat cataatcaac
     2881 taaatctgca acactgttta ttctttttac ataggagtct atactatctg tttgagatat
     2941 ctttttaata tcttcacctt ttacatttcc cccacctcta ggtgttcctg gatttacttg
     3001 tctatttatc tccatagaaa aactatttgt tatattagca aatgtttctt ttgaagctct
     3061 gtccgtagca tctttaatag ataaactaat gagacttaaa gttgacatag ataaaataac
     3121 taatatgata attaatgatt ttaaactttt tctagttaca taagcaaatg cattttttat
     3181 cattattcaa cctccaaatt cattttattt acttttttca actttttacc acttagttct
     3241 aaaatgatat ccgcagaatc tgctacttcc ttactatgtg ttacaactat tacacattta
     3301 tttctatctt tagctaatgt ctttaatata ttaattattt ctccagcagt aacactgtct
     3361 agattaccag taggctcatc agctagtatt attggagcat ctgataccaa tgctctagca
     3421 atagctactc tttgttgctg tccaccagat aatttcataa catttctttt tatttgtttt
     3481 ttatctaaac ctagttcaaa caagatactt tcatctgctg atttatttac taatctaat
//
LOCUS       DAYYCW010000175         3472 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000175 DAYYCW010000000
VERSION     DAYYCW010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.181"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            297..1820
                     /locus_tag="ACHAYA_002864"
     CDS             297..1820
                     /locus_tag="ACHAYA_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8606591.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            2057..3397
                     /locus_tag="ACHAYA_002865"
     CDS             2057..3397
                     /locus_tag="ACHAYA_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8606592.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
BASE COUNT         1106 a          560 c          751 g         1055 t
ORIGIN      
        1 gtttgttttg agtatttttt tgatttatgc ataaaaatca atcaattctt tgcaaatgtg
       61 aaattcttct ccttttaaaa gacgaaaatg tttgtttata aaaaaaagga aacgctacaa
      121 tgaaaaagaa gaaagcttta aaaacctgaa aaaagaggag ggaataggga aaaacgaact
      181 ttttgaacaa aagttttaca aaatgcgtgt tattattttt gttctgatac tctttttgat
      241 aaaataacat tagagccaaa attgttgtct ctaacggaaa agaaaggaag aagaagatgg
      301 aacaaacaaa aaagaaaaaa tttcaaatgc cttcagcgta tacggtctta tttattatta
      361 ttgcagtaat cgctgtactt acttggttta taccagctgg gcactatagt gtagacgatg
      421 caggcaacat cgtcgctgga tcatatgaac gagtacaaca aaatcctcaa gggctgtggg
      481 atattttcat ggcgccaatc aatgggatgc taggtgtacc agcgggggag ctaactaaag
      541 aaacacctgc tgcaatccca atctctttct tcattttggt tgttggtgga tttttaggta
      601 ttatcaacaa aacaggggca ctagatgccg ggattgcttc agttgtgaag aaattcaaag
      661 gaaaagaaaa aatgcttatt ccagtattga tgctgttatt tgcattaggc ggctctactt
      721 acggaatggc agaagaaacg attccattct atgctttact gatcccagtc atgatggctg
      781 taggctttga tacagtagta gctgtagcaa tcgtattagt agggtctcaa gtaggctgtc
      841 tagcttcaac ggtaaatcct tttgcaacag gcgtagcttc acaagctgtt gggatttcga
      901 tgggtgaagg tattggtctt cgattcttga tgttcgttat tttgttagct atttctattg
      961 tatatgtgta tcgttatgca tcaaagatcg aaaaagatcc aacaaaatca ttagtataca
     1021 accaacgtaa agaagatatg aagcatttcg atattgaagc aatcggtcgt caagaagtaa
     1081 tcacgaaaaa acaaaaacat gttaatgtat tgttctttat tacttttggt atcatgatca
     1141 tcagtttgat tccttggaca accttgaatg cgaatttcac gatttttgaa tcattgacaa
     1201 actggttaac aaaccttccg gtactaggta cggttctagg taaaagcatg cttccattag
     1261 gtgactggta cttcccagaa attactatgt tattcttagt aatgtccatc gtagtagcaa
     1321 tcacttatgg catgaaagag tctgaatttg tctctgaatt tttagctggt gcttctgatt
     1381 tgatcggtgt agcaatcgta gtagctgttg ctcgtggtat ccaagtggtt atgaacaatg
     1441 gattaatcac agacaccatc ttacattggg gcgaacaagg attaagttca ttatcttcaa
     1501 gtgtattcat cattattaca tttattttct atatccctat gtcattcttg attccttcaa
     1561 cttccggctt agctgctgca acaatgggaa tcatgggacc tatgggtgaa tttgccggcg
     1621 tcggtaagga tttagtaatc actgcttatc aatctgcatc tggtttagta aacttaatta
     1681 caccaacatc tgctatcgta atgggtgctg tagcaattgc tcgtttcgat gtttcagtgt
     1741 ggtggaaatt taccggtaaa ttaattgcga tattatttgt agccatctgt gtgatcctag
     1801 gtgtagcagc aatgttctaa acctagacaa atacaggtac aaggtctatc ataagaatga
     1861 agaaaccgat aaacggctat cgataagcac gtggataagt caatagctcc attcagtgag
     1921 tgttttttct ttcatcattt gttaagaaca ttctgcttgc tgatggagca ggcttaaaac
     1981 gtcattattt gagaagaaga ggttgtgtcg gaggaatttg acatgaccct ttttaacata
     2041 ttaggaggat aatatcatga aaaaatttgt gacagaacaa catcatgaac aagcattaga
     2101 atcattgagc gaattgatcc gaattccttc cgtactagat gaagcagatt caggacaagg
     2161 acatcccttc ggtaaaaaag tgattgaagc tttggataaa gtattggaaa tcagtgaaaa
     2221 gctaggattt agaacattta aagatccaga aggctattac ggttattcag agattggctc
     2281 tggagatgaa ttattcggta tcttgtgtca tatggacgtt gttccagcag gggacgaaaa
     2341 taattgggaa acaaaaccat tcgatccaac tataaaagac ggctggttag ttggtcgcgg
     2401 atcccaagat gacaaaggtc cttctattgc ggcaatgtat gccgtcaaag ctttgattga
     2461 tgctggcgtg gaattcaaga cacgtattcg ttttatcttt ggtacagatg aagaaaattt
     2521 atggcgttgt ttagaaaaat ataacgaaaa agaagaagga attacgcaag gatttgcgcc
     2581 agatgctgaa tttcctttaa tttatgcaga aaaaggctta ttgcaagctt acttgacagg
     2641 accaggaacg aacgagtttt ctgtaaaagc tggaggagcg ttgaatgttg ttcctgatgc
     2701 agctccttac tctggagaaa agctttctga agtaaaagaa gcattgaaaa aacacgaatt
     2761 cgatttcgaa gaacaaggtg aaggcatcgt cgttcttggt aaaagtatcc acgcgaaaga
     2821 tgcagcacaa ggagtgaatg ctatttctcg tctggcaatt gctctttcag aagtatttga
     2881 ttttggccca atcaatttct taggaaaatt agttcaagaa aatgcaactg gtgaagctgt
     2941 cgtgggcaaa acagaagatg aacaatccgg cgagctaacg atgaactttg caagtttgga
     3001 aatcacacca gaacaaacaa aaatcggtgt agatatgaga atccctgtta cattcaagaa
     3061 agatgattta gtagctaaat taactaaaac agcagaaaaa tatgggttga cttatgaaga
     3121 atttgacttc cttgattcat tatacgtgcc actagacagt gagttagtga aaaatctatt
     3181 ggggacatat cgcgatatta ctggagacat gactgaaccg tttgtatcag gcggcgctac
     3241 ttttgctcgt acgatgaacc agtgtgtggc attcggtgcg atgttccctg atactcctga
     3301 ctttatgcac caggcgaatg aacgatggga attaagtagt atgtataaag caatggaaat
     3361 ttatgcagaa gctgtgtatc gtttatgtgc taaataaaaa taaacataca aaaagggcgg
     3421 atattcaaat ggatattccg tcttttttta tttgatgctg gaaagtttgc at
//
LOCUS       DAYYCW010000176         3388 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000176 DAYYCW010000000
VERSION     DAYYCW010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.182"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..218
                     /locus_tag="ACHAYA_002866"
     CDS             <1..218
                     /locus_tag="ACHAYA_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFM8606593.1"
                     /translation="HYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRY
                     VLTSLLIISLVIYFLKIKNRIIDDYNS"
     gene            231..1238
                     /locus_tag="ACHAYA_002867"
     CDS             231..1238
                     /locus_tag="ACHAYA_002867"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFM8606594.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1250..2254
                     /gene="neuB"
                     /locus_tag="ACHAYA_002868"
     CDS             1250..2254
                     /gene="neuB"
                     /locus_tag="ACHAYA_002868"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFM8606595.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2251..>3388
                     /gene="neuC"
                     /locus_tag="ACHAYA_002869"
     CDS             2251..>3388
                     /gene="neuC"
                     /locus_tag="ACHAYA_002869"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM8606596.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQ"
BASE COUNT         1289 a          428 c          627 g         1044 t
ORIGIN      
        1 ttcattataa tatcgctaaa aaaattacga ataaacaatt atttgacaca aaaaaaatat
       61 gtatcataat aatttttgta tcaatttttt ctttgatact tcatatttta atcaatcaaa
      121 tgttgattcg ttacgttttg acatcattat tgataattag tttagttata tattttttaa
      181 agattaagaa tcgaattata gatgactata attcatagga ggagatataa gtgtttaaag
      241 gaaaaacaat attagtaact ggcggaactg gttccattgg aagtgaaatt gttcgtcagc
      301 ttttaaaata tgaaccaaaa gctatcagaa tttatagtag aggtgaagaa aaacaatttt
      361 atatgcaaca agagctctca aatcacacta atttgagatt tttaattggt gatatcagag
      421 actctcaaag gattgattat gcatgtcgtg atgtggatat tatttttcat gctgccgcaa
      481 tgaaacacgt tccagcaagt gaatataatc ctatggaagc tgtaaaaaca aatgtgatgg
      541 gaactcaaaa tgtaatagat gcagcgatca aaaataatgt aaaacatttt gtaggtatca
      601 gtacagataa agtagtgagt cctactaaca ccatgggtgc gacaaagtta ttgagtgaaa
      661 aattaataat agctgccaat cagtataaag gaaaaacaga aacaatattt tcttgtgtaa
      721 ggtttggaaa tgtcatgggg tcaagtggat ctgttattcc tctttttagt aaacaaataa
      781 aaaacaataa agatattact ttgacagatg ttgatatgac acgatttatg atgtcaatac
      841 cggatgctgc aagattagtt ctgtctgctg ctatgaattc aaaatttgga gaaacatatg
      901 tcttaaaaat gccggcaatc aatattttaa ctttagctca atcattaata aaaatatata
      961 atgatacaca tgatgaaaaa tatagaggtg aaataaaagt tgtaggtatc aggccaggag
     1021 aaaaattata tgaagagcta atgactacag acgaaattga acgctcatat gaaaatgata
     1081 aaatgtatat cattccacca cactatcatg ctactgacga actgtacaaa gattatgaaa
     1141 aaattaaaat cactgcatac tcttcagatg caacagaatt aatgagtgga caggaattta
     1201 ttagaatcgc aaaagagaat aatgtcgtga cttggtaagg agttttatga tgaaatcaga
     1261 agtatatgta attgcagaaa ttggtgtaaa ccataatgga agtctagatt tagctttaga
     1321 aagtattgat aaagctaaag aatgtggagc taacgcggtt aaatttcaaa catttaaaac
     1381 gaataagcta gtttccaaaa aggctccaat ggcaaaatat cagaaggata atcttaaaga
     1441 ggacagttct caatttgaaa tgctaaaaaa attagaatta tctcaatctg attttattag
     1501 aataaaagat tattgtatac aaaataaaat tgattttttg tctactcctt ttgatgaaga
     1561 aagtgcagac tttctaaaac aaattggtgt acaaggattt aaaattggct caggagattt
     1621 aacaaattta ccttttctta gaaaaataga tatgtatggg ttgccagttt tattatctac
     1681 aggtatggct gatattcatg aggtcagtga ggctgtagat tgtttcatag atagtccagt
     1741 tactatttta cattgtactt caaattatcc ggctagttct gaagatatta atttattggc
     1801 gattaacaca ttaaaagaaa tgttcgggat acccgttggg tactccgatc attcattggg
     1861 atatgatgtt ggtatttgtg ctgttgcgat gggtgctaag gtaattgaga aacattttac
     1921 cttggataag gaactacctg gtccagatca taaagcttct ttagatcctc aggaatttgc
     1981 agattttgta aagcatatta gaaacactga aatattttta ggtgatggtg ttaagcgagc
     2041 aatgccttct gaacttagta caaaagaagt atcaagaaaa agcctagtgg tcactgagaa
     2101 tttaaatgtg ggagatattc ttacagagaa atctcttaca ataaaacgtc ctggaaatgg
     2161 aatagctcct aagtttttat atcaatatat tggcaaaaaa gttaaaagac caataagtgc
     2221 agatagtgtt ctgcttgagg aagatgttga atgaaaaaaa tacttatagt aagtggaaca
     2281 cgagctgatt atggaatata taaacctgtt gctgagaatt tactacaaga taattgggat
     2341 gtatcatttg ttgtttctgg aatgcattta tcccataaat atggatttac taaagaattg
     2401 atcttagctg acggatttag aattagtgga gaagttcaaa gtttgttttt agagaacaat
     2461 aagggaaaca tggctcggtc tgtggcatta gagataaatg gtttcacaaa tatatttgaa
     2521 caaactagcc ctgattttgt attattacta ggggatagag gagaaatgtt ggcagctgcg
     2581 atagcttgtt tgtatcttgg aatcaaaaca gcacatattc acggaggcga agtgtctggt
     2641 actgtagatg aatcaataag acatgccata acgaaaatag catctgttca ttttgctgct
     2701 acggaaaaaa gcaaagagcg tttaataaaa atgggggaag attcttggag agtttttccc
     2761 gtcggagctc ctagaataga tactatttta aataatagat tacctagttt tagagatata
     2821 aaagaaaaat atcatctaaa atttgaaaag aaaaaatacg ttttgcaagt atttcatcca
     2881 gttgtaaccg aactagaaaa tatagaaaaa caaattttag ctttgatcaa ttcaatggaa
     2941 caacaatctc aaccaattat ttgcatttta cctaatagtg atgctggtag cgaaattatc
     3001 agaaaaatgt accagaaata tttaaaaacg gatattattt atattacgaa tttagagccg
     3061 gaggaatacc tgactatatt aaaagagtgc aaatttatga tcggaaattc ttcttctggg
     3121 ataattgaag ctgcatcatt caaaatacca gtaataaatt taggaaccag acaaaatgga
     3181 cgagaacaat ctaaaaatat tataaatgca aatctaaatg aaaatgaaat tgaaactgct
     3241 ttgaataaaa tacaagatcc tcagtttata aaaagcttga aaaatgttaa gaacatctac
     3301 ggtgacggca acagttctag aagaataaca aaaacattga atcaaatatt gaatgatggg
     3361 gaagatatta agtggattca aaaacaaa
//
LOCUS       DAYYCW010000177         3348 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000177 DAYYCW010000000
VERSION     DAYYCW010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.183"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            396..710
                     /locus_tag="ACHAYA_002870"
     CDS             396..710
                     /locus_tag="ACHAYA_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606597.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(763..1536)
                     /locus_tag="ACHAYA_002871"
     CDS             complement(763..1536)
                     /locus_tag="ACHAYA_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606598.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            1641..1886
                     /locus_tag="ACHAYA_002872"
     CDS             1641..1886
                     /locus_tag="ACHAYA_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8606599.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            2042..2893
                     /locus_tag="ACHAYA_002873"
     CDS             2042..2893
                     /locus_tag="ACHAYA_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFM8606600.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            2926..>3348
                     /locus_tag="ACHAYA_002874"
     CDS             2926..>3348
                     /locus_tag="ACHAYA_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606601.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVD"
BASE COUNT         1238 a          457 c          576 g         1077 t
ORIGIN      
        1 tagtggctag catatcgtgt gattaagata ctttatagtg taagttgaag gagaaaaaat
       61 atttaaaata tacttcccat ttcagtggga agtaaaatta atcttgttgt ttctactagt
      121 aatgtatctt tttggaaact atgtaacgat gtaaacaaga tttttttcag aagaaaaaac
      181 gataaaagtt gtgataaacg atttaaaaaa taggagaaaa tatatcaagc gcatggtatg
      241 aaacgcacat ttaaaaagat tcgactaact aaaaaaataa gtttacaaag cattgttgtg
      301 tattacaagt gatctataca ctgaaaaatg atagagaaaa tctgatacgt ccgcaacaat
      361 ggacaattga aaatgaaagt gaggaaattg gaagaatggc tgatgaaaat aaagttgttc
      421 cgcttgaaac actagtgata aataaggaag agtttgagca actttttctt gatttttttg
      481 ttcaatatga caggatttat gattttagtt cgtcactaga tttatttgaa ttagaggcag
      541 aaataataga aaacagtcga gaggagaatg gtaaaatcat catttcagta gaaaaattca
      601 atgaattatt ctcagaaaca tttgaaatag atacagatta ttatcaacca caagaagttt
      661 tccaatcatt tagaaatcag attattcgct caggtaagac cgtaaactga aaaacaataa
      721 aagaaacgtt gaaagaataa ttattccttc aacgtttctt ttttagaatg ttttaatgac
      781 caaagcagtt tcattgtcag gcttaggtaa ttcatgaaat cccgtattta tagtgctcaa
      841 ttgatcaact aaccatgttt tactttttga attgtagata aatctaaaat taatatcgtt
      901 attatcgtgt tgaaggggcg tctccaaata atctgctgta tattcatagt caacgtaacc
      961 tgctaataca aaagagagta tccctttatt taatgttaga tttaatggat aatcatacga
     1021 ctttattttt ttcaacttca tattgtacat atcttcagcc ataaaagttg agttcgtgat
     1081 ttcatcttgg gtctcattaa aaaagttatc cgaataatcg cttatttttg aaaagtcttt
     1141 attggcacca gcctcaaaaa attgttcaac tttttcagta taaagttgtg ctaacgctcc
     1201 tttttcaata ttaactttaa tattttcatg tctatcaatt tgtgtgctgt tcagtaaggg
     1261 aagatcagct tttagtttta tatcgtcttg atttctttca atttcaattg gcaaaatctt
     1321 ttcagtagat tcagtttctg ataaaggaac atctagtttt ttatcatgat aataagcttc
     1381 aaatccatct ggaaccggaa aaaaatgtat tgggtaattc ttttctgagg ttgttgtact
     1441 agtttccgta cttgtagaac ttgtgtcaga agtggtacac ccgcttatag ttccgatgag
     1501 taaagcgcaa gttagaatga ttgcaaattt cttcaaaaaa attcctcctt aaaattgaat
     1561 atggactaat actagtccca ttgtagcata ccgaatcttt aagattaata agaaaaagga
     1621 ctaattttag tccaaaaggt gtgctattct tgaaagagga aaaaattatt gataaagaaa
     1681 atgtaatcgg tagcaatatt agacgagtcc gattagaaaa aggaattggt caaaccgagc
     1741 tgattagaga cctacagctt cgtaaaattg ctataactag ggaaacgttg gttaagattg
     1801 aaggtggccg ccaacacatt aagttggatc aattgaaagc catcaaagat attctacagg
     1861 tgtcttatga agatttgctt caatgactac ttgtagtcat ttcgtttgaa attattatgt
     1921 tacaccctta tgtgtcacga agaaatactg gtataataat gaatgggata tgtaacgata
     1981 gggtatactg ttatgttgcc taataaattg tggatcaaaa tacaagaatg gagggatatc
     2041 cttgtataag gcatatgaat tggattttac aaatgctgac ttaaatgcat tctcctcaag
     2101 ctcgcaatca tatacacatg tagtaaatca aatagatcac aaccaaaaat ctattaacaa
     2161 gagaatagaa aatctatttg aaagtgaaaa tgatttagtt gattattatt ctaaggacag
     2221 caaaattcta gatgcagacg aaattattaa agattggttt ccaactataa aagcagatat
     2281 ttttatatct cactctcatg ctgatgaaaa attagctata cgttttgcat catggctatt
     2341 tgaaaatttt ggtcttactg catttattga ttcttcggtt tggggataca gttctgattt
     2401 attaaaaaaa atagaccaaa aatattgcta taaggaacaa acaaaaacat atgattatga
     2461 taaaagaaat gtaacaacta gtcatgttca tatgatgctt tctacagctt taaataatat
     2521 gattgatagt acagaatgtc tgttttttct taatacacct aattcaatta gtttgagcaa
     2581 tgaaatcact aatgaacaga aatttacgta ttcgccatgg ctatatagcg aactgacgac
     2641 agcttcaata gtggaaaaaa agaatccacg attggaaagt aatcctcaaa tgagcaccga
     2701 ggatgttaga agcattataa agcactattc ttcgcaagaa aatctgcaaa taaaatattc
     2761 tttaaattta agtgaattaa cttatttacg aaaagctgat ttgaaaaaat gggctttaga
     2821 tgctgtggaa tgcactaaag gaagtaatac attagatatt ttatataaaa acaccatttt
     2881 cactaatctc taatcaattt actgaatcga aaggaaccgg gtagaatgga taagaaagtt
     2941 ttaataaaga ggttctctga agctattgaa aaaggaaatg cagcaatatt tgcaggtgca
     3001 ggattatcga tgtcacaagg atatgtaagt tggccggaac tacttaatga tcctgcaaca
     3061 gaaatcgggt tggattctaa aaaagaaaca gacttagtta ctctggctca atattataaa
     3121 aatgaaaatg gtggatcaag aggaattttg aatcagatac ttatggataa ttttggtgaa
     3181 gaattagaaa tatctgaaaa tcatcgaata ctagcaagtt tacctattga aacttattgg
     3241 acaactaatt atgatcatct aatagaaaaa tcaatccgag aagcttataa aaaccctcag
     3301 gttaagaaaa attatactca gttagcaaca actaatccaa atgtggat
//
LOCUS       DAYYCW010000178         3308 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000178 DAYYCW010000000
VERSION     DAYYCW010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.184"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACHAYA_002875"
     CDS             complement(<1..2232)
                     /locus_tag="ACHAYA_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8606602.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACHAYA_002876"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACHAYA_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          903 a          741 c          536 g         1128 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaatt
//
LOCUS       DAYYCW010000179         3302 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000179 DAYYCW010000000
VERSION     DAYYCW010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.185"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..897
                     /locus_tag="ACHAYA_002877"
     CDS             82..897
                     /locus_tag="ACHAYA_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8606603.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1023..1280
                     /locus_tag="ACHAYA_002878"
     CDS             1023..1280
                     /locus_tag="ACHAYA_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606604.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1532..2104)
                     /locus_tag="ACHAYA_002879"
     CDS             complement(1532..2104)
                     /locus_tag="ACHAYA_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8606605.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2120..2725)
                     /gene="fic"
                     /locus_tag="ACHAYA_002880"
     CDS             complement(2120..2725)
                     /gene="fic"
                     /locus_tag="ACHAYA_002880"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM8606606.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(3126..>3302)
                     /locus_tag="ACHAYA_002881"
     CDS             complement(3126..>3302)
                     /locus_tag="ACHAYA_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8606607.1"
                     /translation="YLLGMALAYSVGSLIKVNVSLDLFTILLAVGVSSVIGLVFSVMP
                     ASEAAKKDLIDILR"
BASE COUNT         1087 a          611 c          505 g         1099 t
ORIGIN      
        1 ataggataat atattaaggt gctggactac aagtaaaaaa tactgtatga tcatgacaga
       61 ttcaagaaag gagcaataag attggttaag atagcaggac tttctaaagg tggaaaaacc
      121 gtttcacaac tttataaaca aaactacaga gactggagac agttcagaaa aaacattaag
      181 gattcttact ttattctacc aacagaactt gaaaaatatt taccgaataa caaagctatt
      241 aatttatatc tgtattactg ttttaaggca aagaatgaag gcggggattc ctggcattcg
      301 gtcaatacca tagcagagtc tctaggagtg acaactaaaa ctataaacac ctggaataaa
      361 accttagttg atttaggtat gattgcacgt atagacgata accatctcag taaatcgaca
      421 attttacttc ctctctctag ttactataaa ttagcccttg atagttcttt aaaggatgtt
      481 accgagagca ctgtccatga aattgatggc aacttggttg cagtttatca tttgtttgaa
      541 tggagaaaaa atgaggctga tgaaaattat aacgttcctt ataacacact ctgttgtgtt
      601 tttaaaagaa aaacacaaaa agatactatc tataaattta tcttggtcca agatgagaaa
      661 ataagtaatt tcgaattaag cactccttct agtaagattt atgacgatgc atatagattt
      721 gaatgtcaag atctagaagc cctcctttcc gcaaataatc taaacaatat agaacatgaa
      781 aactttgttg taaacactag atttaattta gtttctgaaa aggactcaga tttgttagat
      841 ttgctgatag agcttactaa aaatctagat aagaaagaaa acattactgt actataacta
      901 gcatcggtcc atcattaagt ttggactatt agaacacttg ctattacaga aaaaggtatg
      961 ctagccacgc ttactattta ttggtatcaa acaagtatct taatttgaaa ggaagatgat
     1021 ttatggctaa tttatatata gagaacaaag gagacttgaa tgccattatt actttaatcg
     1081 tagttatggt aaaatcaata ggtaatatca caataggaat gctgtttatt atattagcag
     1141 cagtgctgat ttttatgata gttatttggt tcttaaatga aagatctaaa gacaatcaac
     1201 accgtaggga tatggagata aaatcaatag agtcaataga ttatttatcg ataataacac
     1261 taaaaaagcc acgttcctaa acgtgactta cgctgaacga tatgtgttgt ttggtagacg
     1321 aacaaatcgt tcattataaa tatatgatgg tatcctaata tagtaagcgt ggctagcata
     1381 cctttaatta ctttaccttt tacagttacc atacacattt tttgagcaga aatcaagtaa
     1441 ataaaaaaat attatatttc acaacatgct aatagactca tatctagaac gcattaagta
     1501 tttacctgtc tggttcgctg agacttcttt tttaactttc ttcaattttc ttctttattc
     1561 taaaaagcgt actgcgactg aaaccggtta tagcttcaac ttccttatac gatttacctg
     1621 aagtaagcag ttcatatgcg tgcctcttct tcggtgtgat tgttgctttc ggacgtcctt
     1681 ctctgaaatt aggattattc tttcttgcaa acatttttcc ttcttgggta cgttccacaa
     1741 tcatatcacg ttccatttct gctacactca acaacgtccg tgtcaccatt cgccccatgg
     1801 acgtattttc aatcgttcct agattcaaaa ccttgatcgt tacgttatca tcgagtaaag
     1861 gttcaatgat ctctaatgct tctctcgtgt tacgagcaaa tcgatcaagc ttcgtaacaa
     1921 ctatttcatc tccaggcttc agaccagcca acagctgatt gaattttggc cgatcccgtt
     1981 ttgtacccgt aaatttttct gaaaaaatat tcttcttctc tacgccgttc tcaagcaact
     2041 gttgaatttg ttcttttgta ctttgagaag cggttgagac acgtgcatat ccatatttcg
     2101 tcatttggtc cacctacttt tacaaatcat ctatcgtata ctcagaatac ccttcataga
     2161 aatagctgac atcgattcct ttcatatata actcgcgatc gtcaatttta tcagtcagag
     2221 cattggaaat tagatagcgt atttccaaat cgtttatggg acttcgctcc attgcagaaa
     2281 gatagtctgc tttatcgatt aagttccaat ctacaacttt ttgcaattct tcctttaaaa
     2341 tgagatccag ccaaattcta gtacttcggc catttccttc tcgaaatggg tgagcgatat
     2401 tcatctccac atatttcttg ataatctctt caaaagaagt ttgtggcatc tgatcaatat
     2461 gctgtaacga atgttctaaa tacataactg gtgcaaatcg aaaatttcct tttgcaatat
     2521 tgaccgatcg gatttttccg gcaaaatcgt aaatatccga aaatagaaat tcatgaattt
     2581 cagataaacc tttaaatgta ccaacttcga tttcatttat ttttccacaa tcgtacaatt
     2641 ctttcgcttt ttgtttactg agtttttctt caaccttagc caactcaact tgattggtta
     2701 atcccaattt attctctaaa atcatgcgaa atctccttaa ctttatgaca taagtttatg
     2761 aaacactctc aaccccatta tatcgcgatt ttgtttatgt gtcaaaaact attaagtttc
     2821 tgacccccga tagatttttc aagtgtctct attttgcaca gtctagttga cctttgatac
     2881 acctctatat ttatagagaa tctaaaacat caacatggca tactcaaaat tctaaatatt
     2941 agggataaag aattctccta tacaatcaaa aatttccttt atttcgaact ccggtcacct
     3001 aactctattg atctcttaca agccaaagaa tatctctata ctacaaagaa acaatagggt
     3061 gcctggttgg tctctttgtg ttttatttta gaatctagaa cgtaaatatt cttgtcattc
     3121 tgattttatc taagaatatc aattaaatct ttttttgcag cctctgaagc gggcattaca
     3181 gaaaagacca gtccaataac cgaagatacc ccaaccgcta gtaaaatagt aaatagatca
     3241 agtgacacgt ttacctttat caaagaacca acactataag caagagccat tcctaataaa
     3301 ta
//
LOCUS       DAYYCW010000180         3236 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000180 DAYYCW010000000
VERSION     DAYYCW010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.186"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..302)
                     /locus_tag="ACHAYA_002882"
     CDS             complement(36..302)
                     /locus_tag="ACHAYA_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8606608.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(302..565)
                     /locus_tag="ACHAYA_002883"
     CDS             complement(302..565)
                     /locus_tag="ACHAYA_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8606609.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            1086..1226
                     /locus_tag="ACHAYA_002884"
                     /pseudo
     CDS             1086..1226
                     /locus_tag="ACHAYA_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(1368..3191)
                     /locus_tag="ACHAYA_002885"
     CDS             complement(1368..3191)
                     /locus_tag="ACHAYA_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8606610.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT         1022 a          676 c          587 g          951 t
ORIGIN      
        1 ttgttaacta tctaaaaagg ttggctcttc tcacttcact cataatgtgt ccaggcagaa
       61 taaatttcta caactttatt ttcctcatca actttgtaaa ccactcgatg ctgtctattc
      121 agcctgcgtg agtatctgcc ttcatgtgta ggtctcagct tttcaaaaga ctgcgtgggc
      181 atatatggat cttccttcaa tgtctgaatc acttcttcaa attgtttctt taaattcgtt
      241 tgcttgattt ttcttaaatc cactttcgca gagtttttga ttgcgactgt atagttactc
      301 atcaaagagc ttcccaatca atatcatcga tattcgtaaa gctactgtcg tctttttcgc
      361 ggtctcgtac tttatctaaa gtacctgttt gctcaagaaa aagggtttct tgaatcgctc
      421 tccagtcttc cagcccaatc aatacagcat tgtttccact acgatcacta ataatctcga
      481 tttcagtatg attttcattt acctctttca ataactgata aaaattttta cgagcagtgc
      541 taggattcat cgttgtatta gccattgtct ctcctccttt ttctgatata attatagcgt
      601 acttgattaa gtacgtcaaa aaaatacaat aaagcactta aattcagcaa acttttcctc
      661 ttttttacat agagaaaaag accccttttt agggaggatc tttgtccacg ctcacttctt
      721 actcctttac gactagcggt agccaaacgt aaagtaattg gtaacaacgt atcatcttta
      781 gcaaaggtcg aatccaaagc ctgtgagtct atgcaacaac accaatacac gaaggttcct
      841 cgttgcagtg tgacttgatt gatatcttga ataacctgag gatacgcacg aaagatttgt
      901 aacggttatt caaaactgat tttggccgtc aagtcttcta ctgcctcaaa aatgattgcc
      961 tctttcgtct cctgtcattc atgttcacta tcgatttgat acttgcttag accgttgggt
     1021 ttgtgaatca ccaattaaaa atggcactga gttatggtaa caaaaaatgg tcacttgacg
     1081 aggctgagat tcctacttat gaagacacat ggcacttaag agtcgaggta aaggaagaag
     1141 ctgaaaatat aggaggctgt acattatttg gcaaaggatt cggaaattat gatcaagaca
     1201 tcaaaatcaa aatgttttac agctaaatag gaagaataat aacatttcat acgctgttcc
     1261 catctttaat ggcttataga gggcaccttt agctgagaat aagacaaaac agaccctcta
     1321 gcttcaatta agagctagag ggtctgtttt tgattttaaa acttggttta tttcttcaaa
     1381 tggtagctat aggtgctgac aacgatattt tcacgccaag acaatcgcaa tcttaaccgc
     1441 aaacttgttt ggttatggag aatattctgc cagcgtctgt ttggtacaaa ttgaggaatc
     1501 aacacagtag ttgtatagtt ttgtttcgct gcatttttac ttacgatatc tacataacgg
     1561 ataattggat tttcaatcga gcgataagaa gagtggacga ttgaaaaacg gacatctggg
     1621 aaatgctttt taaattctgc ttcgatttct ttttcttttt cgacatcttc gtctagcgaa
     1681 acatgcatcg caacgacata atcaccgatc gaacgtgcgt aattcaacgc accgatattt
     1741 acccgtgtca cattcccgac taatacaaga acggtattcc cgtcataatc atgtagtttt
     1801 gcatcatttt ctaaacgaag ctgttctgct acttttttat aatgatcatg gatcttataa
     1861 aaaatgaaca taacgattgg cataatgatg aagaaaggcc aaatgtcccc taaccgatag
     1921 acaaacaaaa tcgcaatgat tgcatatgag atgaacgcac ccgtaatatt ggcaatcgat
     1981 ttgcttaacc atctttttcc ttcttttttc catttgacga ccatccctgt ttgagataaa
     2041 gcaaatggaa tgaacacgcc gatagagtat aacggaatca agcgttctgt tgagccatgg
     2101 aaaataaaca gcaatacgat cgaacctagc gctagtgtga taatcccatt ggaatagcct
     2161 agtcgatccc cacgatcttg atacatgtga ggcatgaatt tatcttttgc cagattgtat
     2221 gcaagaactg gaaaagcaga aaaaccggtg ttagcagcaa ctgctaaaat cagcgctgta
     2281 gcaaattgaa ggacgtaata caaaatacca tgtccaaaga cagctttacc aatttgagac
     2341 aaaacagtga cttctttttc aggaacgatc ccataccaat agttgataaa tgtgatcccc
     2401 acgaagaaga aacctaagat caatgccatc atcgtcaatg tagcagcggc attttttgct
     2461 cttggcttct taaagaatgg tacagcatta ctaatcgcct ccaccccagt taaagaggaa
     2521 gaaccagaag aaaatgctcg taaaatcaaa gcgatagaaa cacccggcac agccgctcct
     2581 ggaagtgcag tagcttgcaa tggcacagca cctgtcacga tattatataa accaacaacg
     2641 atcagtaacg agatcacaag gataaatgtg tacaccggaa ataataggaa cgatgcagat
     2701 tccctcagcc cacgaagatt cagcatcatc agtagcaaaa cgattgtcac agaaatagcg
     2761 acttgatgac cgtagagtgc tggcaccgct gatgtgatcg cttcagcacc tgctgagaca
     2821 gatactgcta ctgtcaacat gtagtcgatc aataaagagc caccggaaat caagccagca
     2881 ttctttccaa gattctcact gctgacgaca tatgctcccc cgccatgagg ataagcatgg
     2941 atgatttgac gatatgatag cgttaatgaa atcaataaaa taataacaaa agcggcaatg
     3001 ggtagcgaat accagatggc tgcagcagac aaagcgacta aaacgacaac gatttgttcc
     3061 gtaccatatg caatagatga taacgcatca gacgacaata atgccaaagc agcaaatcgg
     3121 gttaatttgt gctcatcatt ttcagcagat tttaaaggct tacctactaa caaccttttt
     3181 aagtaatcca atttctaaac tcctttacac acaattttac ttcaagtttt atagtc
//
LOCUS       DAYYCW010000181         3231 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000181 DAYYCW010000000
VERSION     DAYYCW010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.187"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            627..959
                     /gene="traE"
                     /locus_tag="ACHAYA_002886"
     CDS             627..959
                     /gene="traE"
                     /locus_tag="ACHAYA_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM8606611.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1578..1919
                     /locus_tag="ACHAYA_002887"
     CDS             1578..1919
                     /locus_tag="ACHAYA_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM8606612.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            complement(2156..>3231)
                     /locus_tag="ACHAYA_002888"
     CDS             complement(2156..>3231)
                     /locus_tag="ACHAYA_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8606613.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         1036 a          482 c          667 g         1046 t
ORIGIN      
        1 gaaaagggaa cgtatgtttg catactcctt ttttttgagt tataataata cgcgaagaga
       61 gataatttca ttatttaaac aagctaaaaa tcttgatatt acttcttttc aggcaatacg
      121 aatattattc gcttacattt tttgtataaa tttaattgtc tgctatgctt gtgatgaatt
      181 gattttcgtt ttgcactttt ggatgataaa tttggacaat tgaaaataca aaaaaaagaa
      241 aggcggtatg aactcggaag gctggtaacc ttcgtcagcg ttcatacacg gcattaagat
      301 ttggccctta atgcgttaac ctttcagtgt tacggtatta cccgcaatgg atttagtata
      361 acagatattc aaaagtgata ccactttttt tggctgtgtt aaggttaaaa atacgtgata
      421 ttacgatgaa tctatgccta agcataatgt tataaaaatg tccgtgtggc tctttgccgt
      481 atgacgaacg ctgaaattag agggaacgca tgtttggatg cgttccctct tttttgtttg
      541 tcatgaacga aaatcaattc tctatttttg aaaaataaca agaaaaactt gttaaaaata
      601 tttaaagaaa ttgggtggtt tattttatgt catatacagg aattttgagt ctcaaagata
      661 tttgtcacta cggtaaacgg tgtaccgcta cagagaaaat cacgaagaaa ctatcaactg
      721 gccaaagtaa aaccgttgtg cagtgtaaga aatatataat ccagaaagac aaagtctcag
      781 aggaaatgat ttattacatt gggaaacaaa agcaaataat tttaaaagat cctatcccct
      841 taaaagagct atatccaaca atcaaacatg tttatgacca aaacggtgta ctgattggtc
      901 gaagaaaaaa tggagtcttg cgttgtaccg caaaagggat ggggcgtttg atcggctgaa
      961 ttgatcattc tcataacttg tattttatcg tttttcgctt gcttgaaaat attgtcgaga
     1021 cttctaaagg aacttagagt cactagaaaa ataaggagta gacttgatct atcaacaatt
     1081 cccttcgggg agtgacgggg taggttttaa cgattggtaa cgtaaaaaat tggtcaatcg
     1141 agtaaatgcg cttgtttgga tagtggtgaa atgaatttgt gtaaataata agcgcagagt
     1201 aaacaagagg gaatttagaa gttagacaag ttagaatagc gtctagatta atcatgagca
     1261 gctactaagg gatctagact ttgacgcaaa ctcatggaat aaggagtagg tcttctcgta
     1321 atggggagga cgcacaagaa attgtgtcgt gtgctgcagt acatgtagat acttgcgagg
     1381 acgttgatta ttggatcaat cgagcattta ccgctgtcta ctagggtttc tcgtttcgga
     1441 cgccataaac gagtcgaaat tttttatgta tttttaagta aacgaaaaac gataaaatat
     1501 gaacaagtcc tgaataaagt gaaaaaattg atataaaaca tagagaaaat gaaatttacg
     1561 taatggagga atgacaaatg aaaaattatg atccgaatat acgattgggc acacatacta
     1621 ttaaggtttc tttccaacgt tgggattaca aaggatttgt gacgtttaga aggggcggaa
     1681 attgtaaagg attagatgtt ttagcattag acgaggatga tttatacgat caaacattga
     1741 ctgataatcc aattggattc ggcttgctac ctgaagatga tgaaggtaat gaatggttta
     1801 aaatgacgct tatgaatgat aatggagacg aattatctgt tgaagatact tggtcatatt
     1861 tgagtgatta tattgtcagc tttgaaatca ttgaatttgt agcggataaa gaagaataaa
     1921 aactggagga ataaatttta cagataaagg cttagaagga gtcacgacga ttaataaaaa
     1981 ggtagtatta ccttaggcat tgagtaagga atttatttag tctaatggtg ataagtcaag
     2041 atgaaattta agattttctt tattgagtaa tatgttttga agcgcacgaa actagacccg
     2101 atcaaaatca aatttttttc gggttgattc aaggatttgc gtcaataacg gattgttatt
     2161 tctctatcac actccttggt ggcgtataga gttggtgttt acgtagtagc gtatccacca
     2221 gacgcgtaaa ttttcttgcg gttaggacga gtgcacgttt gtgttgatgc tttggaacct
     2281 ccttatattt actttgataa aaagctttgt attcaggaag ataatttttt acagagttgg
     2341 cagcttcaac taagtagtat ctaaaatatc gatttccttg ttttgtcaga ggtgtatttt
     2401 gcgactgata gtttccagat tggttcactt tccaacttaa tccagcgtat tttgccaatt
     2461 ttgtctgatc ttcaaatctt tcgatttgac caatttcagc gattaaaccg gcagcataga
     2521 ctttaccaac gcctggaata ctggttaagc attgatattc tggaatgaca acaattactt
     2581 gttcgatggc tttatctaat tctttgatat ttttttcaag tgctcgtatt tctcgtacga
     2641 gaacacttaa aacaacatta attgattctt gtgcaagagc acctaggcgg taactcatgg
     2701 taattgcacg ttgaattgct ttagcgattt tttctggttg cttgaaacgg ccttttcctt
     2761 tttcttgaag taaatcacag aaagcttcta aaggaagctc agcaagttca tccaatgtga
     2821 aatcttccgt catgagtgaa atgagggttg cactaaaaag actcgtcgat tcgtctcgca
     2881 tttctcgagc aagcgtatta catttgtacg ttagattttc aagaaaatgt tgctttgtac
     2941 gaatcaattg cttaataagt tgatagcgag tacgtgttaa acgctgtaag gccatgtatt
     3001 tctcttcttt gataggggaa gttgtgaatc gttgaatacg caagaaatca gcgattctta
     3061 atgcatcgtt tcgatcagtt ttattctcat caaacatgcg actaaattgt ttcacccgaa
     3121 aaggattttc aatcgttaca agtgtgttga gcttctttag tttctcatct tcatgaaaaa
     3181 acatggaagg atggaagctg tacatggacg ttgactccat gccaatcaca a
//
LOCUS       DAYYCW010000182         3219 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000182 DAYYCW010000000
VERSION     DAYYCW010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.188"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(554..2167)
                     /locus_tag="ACHAYA_002889"
     CDS             complement(554..2167)
                     /locus_tag="ACHAYA_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8606614.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2308..3216)
                     /locus_tag="ACHAYA_002890"
     CDS             complement(2308..3216)
                     /locus_tag="ACHAYA_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8606615.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1038 a          552 c          572 g         1057 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataagtattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag
     3181 attggaggat tggttgtttg ttaacaaaag aaccacttt
//
LOCUS       DAYYCW010000183         3196 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000183 DAYYCW010000000
VERSION     DAYYCW010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.189"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(248..616)
                     /locus_tag="ACHAYA_002891"
     CDS             complement(248..616)
                     /locus_tag="ACHAYA_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM8606616.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            766..1248
                     /locus_tag="ACHAYA_002892"
     CDS             766..1248
                     /locus_tag="ACHAYA_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM8606617.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1285..2295
                     /locus_tag="ACHAYA_002893"
     CDS             1285..2295
                     /locus_tag="ACHAYA_002893"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM8606618.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2410..3123)
                     /locus_tag="ACHAYA_002894"
     CDS             complement(2410..3123)
                     /locus_tag="ACHAYA_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606619.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         1079 a          517 c          621 g          979 t
ORIGIN      
        1 ctcgtatttt ttccttattc aaaagttttg caggaaaatt gctgaaaaaa taattttact
       61 aagtggaaaa ttcaaagcca ttcaatcagt gatttttttc agcacaagaa agactcctta
      121 taaaaatagc agatactttg tcaattaagt gtttaaccag ggatgataaa aaggatagga
      181 ttgaacaaaa cctgtataag ctgatgaata gtttcctcgc cagtaaaagg tttattcgta
      241 taaagcttta tcgccaaaat ttatcatgac cttttttatc ttcaatatta aatgaaatca
      301 attgttcaat caattcccag ttgattacac ttttccaagg aactttgatg acgttatccg
      361 tatatacgta gcctgcttct tggatatttt tttcaagggc acgaatcgct gctttttccg
      421 gtgcgataga aaaatgattt ttagcaaaac taaatgcaat aataaaggta ccatggtcag
      481 taaacatagg ttggttccat tttacagttg tgtctagctg gggatacgtt tctgttatct
      541 tagcaaaaag gtctttgatc ttttgttgat gttctttttc tttgatctga ttgatgatcg
      601 gctgaaattt ttccattcta aaacgcctcc attaagtggt ttgatttatt ttaccagaaa
      661 aacaatagtt atgtataaaa aacgaacaaa acacaagtaa tgttgtaacc gctatattta
      721 tatgataaga tgtctcaaat aataaaatag aaaagagaca taaaaatgat tgacttggca
      781 aagaaattct tacacgaaaa tatatatttt gagttgtatc atcatcgagc catttataca
      841 aatgaagatg ctttggtaat aaagaaagaa caaggattta caggtacaga gaccaaaagc
      901 ttattcttaa aagataaaaa agataataac tacgtatatc tgacttttac tacgaaacaa
      961 acggatttca aagcattagg gaagatcgtc ggtaaaaggc tttctattgt ttcatcagaa
     1021 cgcatggaag aacaaacggg acaaaagcag ggcgctgttt ctccgtttgg ttatgaaaaa
     1081 cagattccga ttattattga tgaagagctc ttatcaaatg aaaaacttgt ttttgcacca
     1141 ggtagaccag atcaaacgat ggtcatccaa acggttgact taaacaaaat catccagcta
     1201 cttgagatgc cgatattttt gatgccggca aacgaaaaaa acaattaacc aagtaaaata
     1261 gtgcatactt ggaggtgaag caacatgata agttatccat tattagaaag aaatgcaacg
     1321 attggggtta ctgcaccatc ctcaggcgta tcagaggaac accattcgtt actcgaatca
     1381 gctttaaaga gaatggaaga aaaatctttc caagtagtta ctggaaagac gctttggacg
     1441 caagataagg aaaaatcagg tccagcaaga aaacgagcag atgaattcaa tcaaatgatg
     1501 caagatgaaa atatccaaat gatctttccg ccttggggag gagaattatt gattgagatt
     1561 ctcgattttg tcgattttga tgctctacaa ccaaaatgga tagtaggtta ttcagatatt
     1621 agtacgttgc tacttgctgt cacattaaaa acagggatag ccactgcaca tggaaccaac
     1681 cttattgatt taagaggaga gtacttggat gaaacgacag caatgtggga aagtgtactg
     1741 aaaacaagag aaggagaatc gattctacag caaccttctg aaaagtatca aaaagagtgg
     1801 ccccatgagc attcatcatc ttctgttttc catctcactg aaccaacaaa ttggcaatca
     1861 gtcagcggag atgcagtaag gatgaaggga cgattattag gtggctgtat tgatacgatc
     1921 agtcatttaa tcggtacccc ttatggagat gtccaatctt ttaaaagaaa gtataatgat
     1981 ggaaagccga tcttgtggtt tctagaaaat tgtgagctat cgtcagttga tttaagaaga
     2041 tcacttgttc agatgaaact ggcaggatgg tttgatggca gctcggggat catgttcggt
     2101 agaagtgcag cagaggattt gttagattat aaaatgaagg atgtatatga agatctgtca
     2161 aaggaattaa aactaccgat cttgtatgat attgattgtg gtcatgtacc gccacaactg
     2221 acattgatca atggtgccta tgcagaagta gcggtagaag atagaaaagc gactgttttg
     2281 caaacgttta tttaaaaaat tttttcctgc cttggagttt atcaaagacc aacaaatcta
     2341 aatagaaaag aaaatgaaag aagctgacag tattttgcca gcttcttttt gtatgatcta
     2401 ttagaaattt taattatcca taactatcac attgatcagc tgtttcacat catttggatt
     2461 acctgtagga atagctacta cagatgcatt atcaataatt tcttgagttg tttctctaat
     2521 gaacgctgga taagatgttt gatctgcagg catttgccaa tgtgaaggaa ctggataact
     2581 agtgattgtc ccgtcatggt tacttgaata cactaaaact ccctggtaac tgtatttccc
     2641 agtgagcata accacatctt tcggatagac tgcactccct gcatcataag gggcgatagg
     2701 tgttcctgca ggataatgaa taacatgtag ttcgaaatca cccgatgtat ctctaaaagt
     2761 attacctcta attgctagcc aaacacgtgc gtactcaatt tgtagatcag aatatcctgc
     2821 taacggatct attgactgag aagactgtga ggaagttgtg tctattgttg aagaagaaat
     2881 atttgtctca actgtactac tagatgtaga agaagtattg ggctctgaga ctttactgct
     2941 tgttgaagat gtattcgttt cagtagaagc tgatttctga gtacttgatt tttcagctga
     3001 actggtttgt ttgattgaac ttttgtctgt ggtcgcagat ggatcactat tttctttact
     3061 aggagacgta catcctacaa ggaataaaga gaccaacaat aaaataaaac ctaacttttt
     3121 catataaaat acctcattat ttttttaata ccagtatagc ataatcctga atttttgaat
     3181 ccgtaaaacc ttgtct
//
LOCUS       DAYYCW010000184         3026 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000184 DAYYCW010000000
VERSION     DAYYCW010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.190"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..464
                     /locus_tag="ACHAYA_002895"
     CDS             <1..464
                     /locus_tag="ACHAYA_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM8606620.1"
                     /translation="HQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINYVI
                     NTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAIIQK
                     LYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            471..2963
                     /locus_tag="ACHAYA_002896"
     CDS             471..2963
                     /locus_tag="ACHAYA_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM8606621.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
BASE COUNT         1065 a          504 c          675 g          782 t
ORIGIN      
        1 cacatcagaa tacctcggat ttaattgaat catatataaa gaaaattctg gaaaatagtg
       61 aaatgatcga gattcgaagg attgaaatgg cagacctctt taactgtgtt ccttcacaaa
      121 tcaactatgt gattaatacc cgttttacca tccaacgtgg ttatctagta gaaagcaaac
      181 gtggtggtgg tggttatata cgaattgcaa aagtaaggat ttcagataaa aaacaattac
      241 ttgagcagat aaatcagtta tttgatgata caatcagtga gaaaaatgct tttgctatta
      301 ttcaaaaact ttatgaagac caaataatta caaaaaaaga aggaaacttg atgttgagtg
      361 caattgcaaa aaacacattg aatttcaatg aatatgaaga tcatacacgt gctcggattc
      421 tccgagcatt tctggaacgt ttaagttatg aagatgggaa gtgagattag atggaagaac
      481 tttttactga aagagcccaa gcagtattag aaattgcaca agaagaagca aaacggttga
      541 aacatcaatc agttagttca gaacatgttt tactagcctt agtggtcgag caaaatggaa
      601 ttgctggaaa agtattacgt gaaatggatt tgaatgaaac ggaaatttat gaagaaattg
      661 aacatctgat tggttatggt ggtatccgtt cttaccctaa aggagtcttt cttccatatt
      721 caccaagaat gaaacaagtt tttgctttag ctagcagcga agtgaagaaa actggttctc
      781 aaaaagttgg aacagaacat attttgatga gcttattaaa agatgaaagt atcatggcta
      841 ctcgaatcat gatcaactta ggtatcagcc tttcaaaagc ccgtcaagtt ctgaaacaaa
      901 aaatggggct agctggtgat tctgctggaa aaggaatgaa tcgtcgtaga aatgtaaatg
      961 tacaagataa acggcaaaca ccgcaaggaa cacctacgct agattcatta gcacgtgatt
     1021 tgacgaaatt agcaaaagaa aacaagctgg acccagttgt tggtcgttcg agagaagtga
     1081 aacggcttat ccagattctt agccgacgga caaagaataa ccctgtactg gtgggtgaac
     1141 ctggtgtagg caaaacagcc attgctgaag ggttagctca aaaaatcatt ttaggtgaag
     1201 tgccagaaga catgcaagaa aagcgcctga tgatgcttga tatgggatct ttggttgctg
     1261 gtacgaaata tcgcggtgaa tttgaagatc gaatgaaaaa ggtcatcgac gaaatctata
     1321 atgatggtca agtaatctta ttcattgatg aattgcatac cttaattggt gcaggtggag
     1381 cagaaggagc aattgatgct tcgaatatct tgaaaccggc gttagctcga ggagaattac
     1441 aaacgatcgg agcaacgacc ctagatgaat atcaaaaata tatcgaaaaa gattctgcat
     1501 tagagcgacg ttttgctcgc attcaagtag atgagccaac tcctgaagaa gcagaagtga
     1561 ttcttcaagg attacgtaca agatatgaag agcaccatgg agtggagatc acagatgaag
     1621 ctgtacgagc agcagtcaat ctttctgttc gttatatcac ttctcgtcaa ctacccgata
     1681 aagcaattga tttgatcgat gaatcagcag cgaaagtccg tttggatcaa accgatcact
     1741 taacaaaatc aactgtgatc aaactagaaa tcgatgaact agtacaagaa aaggaagcag
     1801 cgatccaaaa acaagacttt gaaaatgctg ctcaattacg cagacaagaa aaagcactac
     1861 gtaaaaagct gcaaaaagtt tctgcaattg aagcaaaaca ggagcaaggt tattctgatc
     1921 gtgtaacaga agaagatgtt gctacagttg tatcggagtg gactggtgtc cctctacagc
     1981 agttggagaa aaaagaaagc gaacgcttgt tagaactgga aggacttctg catgaacgtg
     2041 tcgttggtca agatgaggct gtcaaagcag tttcacgtgc gatccgccgt gcacgaagcg
     2101 gattgaaaga tcctgatcgt ccaattggtt cattcatgtt ccttggacca actggtgtcg
     2161 gaaaaacaga attggccaaa gcactttcag aagtcatgtt tggaagtgaa gatgccctga
     2221 tccgtgttga tatgtcagaa tttatggaaa aatacagtac aagccgatta atcgggtctc
     2281 ctccaggata cgtagggtat gatgaaggtg gacagttgac agaaaaaatt cgtcaaaaac
     2341 catattcagt gattctctta gatgaagtgg agaaagcaca tccagacgtt ttcaatttac
     2401 ttctgcaagt attggatgat ggtcatttaa cagattctaa aggtagaaaa gtggacttta
     2461 gaaatacaat catgattatg acttcaaata tcggagcgac acaaattcga gaagaaaaaa
     2521 atgtcggatt caatgtccaa gatgttacta aagatcacaa agccatgcaa aaacgtatct
     2581 tagaagagtt aaagaaagct ttccgacctg aattcttgaa tcgaatcgat gagactgtcg
     2641 tcttccattc attgagtaaa gatgaaatcc atacaatcgt ccaaatcatg agcaaagcga
     2701 ttatcaaacg attgaaagaa caagatattc aagtgaaaat cacaccatct gcaatcgatg
     2761 tgatcgggaa agctggattt gatcctgaat atggtgcacg tccaatccgt cgcgcacttc
     2821 aaaaagaaat tgaagatcga ttgagcgagg ctttgcttgg cggagaaatt cgtttaggtg
     2881 accacgtaac agtaggtgct tcaaaaggta agatcacatt gaatgttcgc ggacctaaaa
     2941 aggaaacagt aggttcccta tagttacttg gcctgtgtag ctgaattttt ataatagtaa
     3001 gacttgatta agaggttgtg acaaga
//
LOCUS       DAYYCW010000185         2951 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000185 DAYYCW010000000
VERSION     DAYYCW010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.191"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1610
                     /locus_tag="ACHAYA_002897"
     CDS             <1..1610
                     /locus_tag="ACHAYA_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8606622.1"
                     /translation="TDNVTNLPTLPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDG
                     TKDTVKVPVEVTDNRSDADKYEPTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVT
                     PGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGG
                     TVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVE
                     VTDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNK
                     KGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKED
                     LPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPIT
                     EGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESG
                     NTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSN
                     IFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1744..>2951
                     /locus_tag="ACHAYA_002898"
     CDS             1744..>2951
                     /locus_tag="ACHAYA_002898"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8606623.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLS"
BASE COUNT         1158 a          417 c          591 g          785 t
ORIGIN      
        1 taacagataa tgtaaccaac ctaccaacat taccagaagg aacaacagta acagatgtca
       61 ctcctggtgg tacgattgat accaacacac caggtaatta cgaaggggtt atcgaagtaa
      121 cgtatccaga tggtacgaaa gatacagtga aagttccagt agaagtgaca gacaatcgtt
      181 ccgatgccga taaatatgaa ccaacagtag aaggcgaaaa agtagaagtt gggggcacag
      241 tagatctaac agataatgtt actaacttgc cagcgttacc agaaggaaca acagtaacag
      301 atgttactcc cggcggtaca attgatacca acacaccagg taattacgaa ggtgtcattg
      361 aagtaacgta tccagacggt acgaaagata cggtgaaagt tccagtagaa gtgacagaca
      421 atcgttctga cgccgataaa tatgaaccaa cagtagaagg cgaaaaagta gaaattggag
      481 gcacagtaga tttaacagat aatgttacta acttgccagc gttaccagaa ggaacaacag
      541 taacagatgt tactcccggc ggtacaattg ataccaacac accaggcaat tatgaaggtg
      601 tcattgaagt aacgtatcca gacggtacga aagatacggt gaaagttcca gtagaagtga
      661 cagacaatcg ttctaatgca gataaataca atcctgaagg acaaaaagtg actacagact
      721 taaataaaga gccagatgca tctgagggaa ttaagaacaa agaagattta ccaaaaggaa
      781 ctacttatgc ttggaaagag aaagtagatg ttagcacagc tggaaataaa aaaggtacta
      841 ttgtagtgac atatccagat ggatcatctg atgaagttga agtagacgtt acagtgacag
      901 atactcgttc cgatgcagat aaatataatc ctgaaggaca aaaagtgact acagacttaa
      961 ataaagagcc agatgcatct gagggaatta agaacaaaga agatctacca aaaggaacta
     1021 cttatacttg gaaagaaaaa gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag
     1081 tagtaacata tccagatgga tctaaagaag aagtagaagt tactatttct gtagtagata
     1141 aaaaagcacc gaataaacct caagttgatc ctattacaga gggtgaccaa attgttactg
     1201 gtaaaactga accaaatgca gaggtgacag ttacattacc agacggaagt caacaccatg
     1261 ggacagctga taaaaatggt aattttacag ttaaagttcc taaattagag gcagggacaa
     1321 aagtgatagt aactgcaact gatgaatctg gtaatactag tgaacctact aatgtagttg
     1381 tttcttccaa caaaaaagac agtggaaaaa acggtagtaa aggtagtaag actgacaatc
     1441 aaggtagcaa cagcaatcaa gacaaaaata gaggcaaaag ccagtcctca aaagtttttc
     1501 ctaagacggg agagagtgac agtaatatct ttactattag cggtggttta atactgttag
     1561 gaacgttagg attattaggg tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt
     1621 tcttaagtct tctctgagaa gaggaagaat ctttttcttc ctctttttgc ttttttatga
     1681 tgggaaataa gtgttagtta aagatgaata tactttttat agaaaggaaa agaatgtaat
     1741 taagtggaaa aaatagtaat tattggttca aaccattcag ggctagccgc agctcgattt
     1801 ctaaacttaa gcgattcaaa ttatgaagtt attctaatcg ataaaaaatc agaaattagt
     1861 tacttggaat gtgggacatc gttatttata aaaggagaag taagtaattt agaagatctt
     1921 ttttatcaaa atagaatgac actaaattct gagataaaaa agttatttct agagacagaa
     1981 gttgacaaaa ttgatattca aaataaaaag attagttgtg taaaaaaaga tggtacaaga
     2041 ttctctcaag aatatgacaa agttatatta gctacaggtt caaagcaaat tatgctagat
     2101 ataccgggga ataatttagc taatattttt tcgataaaga attttccaaa tgcaaaagag
     2161 atttttaaag ctttggatga tagtactatt aaaaaagtag gaattattgg agcaggatat
     2221 attggagttg aaattgctga agccataaga aaaagaggga aagaagtata tcttttcgat
     2281 gttgcagata gagtgttgtc gacttattac gaccgtagtt tttcagataa agttgaagaa
     2341 atactatcaa aaaatggaat acatctttgt ctatcagaaa gtcctaaaaa atatttagga
     2401 agaaaaaaga ttgaaaaaat agtcactaca agtggtaccc aatactctat ggatatggtc
     2461 gtacaagcta taggatttct atctaatagt ccaattgggg aaaaggaact cttgagagat
     2521 gttagtggag catatctaac taatgaatac cagcaaacaa atataaaaga tatatatgct
     2581 attggggatt gtgctacaac tttttttacg tccattaata gaagtagtgt tgatttttct
     2641 atttctaatg ctctaagaac agcttatatt gcaactcaac atattaacaa tcaagatttt
     2701 acaccttctg gttcccaatt tacaaatggc ttttctatat ttaattacaa tttttacgca
     2761 gcaggtttga atttaaaaac agcacgatta ttaagtgagt ctatagatta tatagaaata
     2821 gaagatttca ttagacctgc ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc
     2881 tttgaaaaaa atacaaagag aatagtaggt gtacaacttt gtagtcaaga agatttatct
     2941 ggactattat c
//
LOCUS       DAYYCW010000186         2906 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000186 DAYYCW010000000
VERSION     DAYYCW010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.192"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            580..>2906
                     /gene="esp"
                     /locus_tag="ACHAYA_002899"
     CDS             580..>2906
                     /gene="esp"
                     /locus_tag="ACHAYA_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM8606624.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1058 a          442 c          567 g          839 t
ORIGIN      
        1 aattcaatta taaagaaaaa gaatcaataa tttaatgagg ctgtgacaaa tgtcattttt
       61 tgtttcgcta aaaacgagtg aaaaaatgtt gataactcca atcattcgtt taagattcta
      121 ggaatcattc agaaatgctg tgaaaccgat actttataat tataaagaag gtgatttcaa
      181 attccgaaga aggaatttga agtcaccttt ttacttatgt cacagtctca ttcagtaatg
      241 cgggataagg ctccttattc cgttatttga atactctgaa gtttaatcta agaaagggta
      301 atattttgaa aacatgcttc atcgcataga aatgaactta atttcagaaa gaatacatac
      361 atatcttttt atatgttttc agctgctgaa tggttaaaaa gcacaaatat ccgaaaaaaa
      421 catgaagaaa tgtacttttt ttgttgtaat gttcatgaaa ttctctaagt aaattagtta
      481 acataaggtt agtaagattc ctattctgtg aataatacgt atcagattta tcttttattc
      541 ttactaggaa agtaattatt aagggagttg atttgaataa tggttagcaa gaataataag
      601 agagtatttc tagaaaaaac aaagaaacga gtattgaagt acagtatcaa aaaattaagt
      661 gttggggttg catctgtttt gattggtgtt ggtcttgtct tgggaacaac tgaactagtt
      721 aaagcacaag atgaaatttc tccaagcagc ccacttgaaa cagcgataag ctccgtacaa
      781 gtaggtgaca aagtagcttc ggggaatact tttcaggaga atcctggtta tacgaaaaat
      841 tataactttt cggatttaca atttagtcct caagaactaa ctggagacac actaaaaggc
      901 aatacaattg gatttgaagt ttacggaaaa cataatattg cagcatcaac taaaaactgg
      961 gaaatccgtc ttcaattaga tgaacgatta gctaaatatg tagaaaaaat tcaggtcgat
     1021 ccaaagaaag gaattggatc ttctagacgg acctttgtaa gaattaatga ttcgctgggt
     1081 agacctacaa atatttggaa ggtcaattat attcgtgcaa gtgatggatt atttgctggg
     1141 gcagaaacaa ccgatacgca aaccgctcct aatggggtaa ttacgtttga aaagagccta
     1201 gatgaaattt ttaaagagat aggcatagac aatctcaaga ccgatcgttt aatgtatcga
     1261 atctacttgg taagtcatca agacgatgat aaaattgtac cgggtattga tagtacgggc
     1321 tactttttaa cagattcaga tgatttctat aatagcttag atgtatctga aaataatcct
     1381 gaccagttta aacatggatc agtaagtgca aaatatgaga aacctaatac tcaaacaaaa
     1441 gatggttcag gatccacagg tgctaatgga gctataatct tagatcataa gttaacgaaa
     1501 aattataatt tctcatattc atcttccgca aagggcacgc cttggtatgc taattacaaa
     1561 attgatgaac gattagttcc atatgtagcg ggtatacaga tgcacatggt tcaggctgac
     1621 aaagtaacat atgatgtttc gtttgaatcc ggaacaaaag tagctgattt agcaatcgag
     1681 cgacgggaag gtcatgaaaa ttatggcata ggttcaataa ctaataatga tttaactaaa
     1741 cttatcgatt ttgctaatgc aagtccacgt ccagtcgtta ttcgatatgt tttacaattg
     1801 actaagccat tagatgaaat cttagaagat atgaaagcaa cagcacaagt tgaagaaaat
     1861 aaaccatttg gtgaagattt catctttgat tcttggttgt cggatacgaa taaaaaatta
     1921 atccagaaca cttatggaac aggttattat tatttgcaag atattgatgg tgatggaaac
     1981 cctgacgata aagaagagag cggagacacg aatccatata tcgggaaacc tgaattagaa
     2041 gaagtatatg atgttgacac aacagttaag gggaaagtat tcatccacga gttagcggga
     2101 acaggtcaca aagcccaact tgttgataaa gaaggtactg tattagcaga aaaaactatc
     2161 gctccaaatg aaaaagatgg ggctccaatt tcagatactg tagaatttga atttacgggt
     2221 gtagattcaa gtaaattaat cgcgaaagat gaattaaaaa tccaaatcgt ttctccaggt
     2281 tttgataaac cagaagaagg ttcaaccgtt attaaggaat caccaaaagc ggttgataaa
     2341 caaaccgtgg tagttggatt taaaccagat gctaaagaat caattcggaa taataaaaac
     2401 ttacctgaag atgcagagta ttcatggaaa acagagcctg atacttctaa cgttactgat
     2461 agtacgaaag gtattgtaac tgttaagatc ggaaatcgaa ctttcgacgt ggatgtagag
     2521 tttgctgtaa aagcttctca agctatggaa aatgatgcaa catacgtacc tataacaaca
     2581 actccagaaa cgacagttca aagtggaaaa cctacatttg ataaaccaga tgttccttta
     2641 gctaaagatg ccttttcaat ttcagatgtc tataataagt acttcggtaa tgcaagtgtt
     2701 gatgcaaaca ctggtattgt tacattcact ccagctaaag gtgtaggaga atcggagccg
     2761 attactggaa caattcctat taaaattgtt taccaagatg gttctgtagg cacgaccgat
     2821 ttagcagtaa ctgtaagtaa agatatttat gaaaatccag gagaaaacat tcctgcaggc
     2881 taccacaaag taaccttcac cgcagg
//
LOCUS       DAYYCW010000187         2847 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000187 DAYYCW010000000
VERSION     DAYYCW010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.193"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACHAYA_002900"
     CDS             <1..1087
                     /locus_tag="ACHAYA_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8606625.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="ACHAYA_002901"
     CDS             complement(1305..2447)
                     /locus_tag="ACHAYA_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8606626.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="ACHAYA_002902"
     CDS             complement(2452..2637)
                     /locus_tag="ACHAYA_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8606627.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          945 a          523 c          446 g          933 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaa
//
LOCUS       DAYYCW010000188         2818 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000188 DAYYCW010000000
VERSION     DAYYCW010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.194"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            188..511
                     /locus_tag="ACHAYA_002903"
     CDS             188..511
                     /locus_tag="ACHAYA_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606628.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            675..929
                     /locus_tag="ACHAYA_002904"
     CDS             675..929
                     /locus_tag="ACHAYA_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606629.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            999..1244
                     /locus_tag="ACHAYA_002905"
     CDS             999..1244
                     /locus_tag="ACHAYA_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8606630.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1320..1685
                     /locus_tag="ACHAYA_002906"
     CDS             1320..1685
                     /locus_tag="ACHAYA_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM8606631.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1746..2309)
                     /locus_tag="ACHAYA_002907"
     CDS             complement(1746..2309)
                     /locus_tag="ACHAYA_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606632.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          835 a          493 c          432 g         1058 t
ORIGIN      
        1 catcataaat aaaatttatt tttgttactt ttcaactata gcacgaatga caaactctca
       61 caagtttcaa aatataagta aaataatatt gttattctta tttcttttac ttgtgtcata
      121 actattttgt tactttaatt tttatttgct tattttagaa aaaatgttat tcttttattt
      181 attctcaatg ttaggagtga ttattttgcc atttattgcg attttattcg atttagttgc
      241 ggcagcagct tattttctcc aattaaatta tcaaacctca ggaattctgt ttattggaat
      301 gatttttcaa ggtgtgatta cccttttgct tcttgtcatg atgctcagct ataaaggaaa
      361 aagatatgcc cgtattcaaa caaaagtatt tgttaaatat gtttccattc gctatgggat
      421 catcattctt tcctttttag tgaatgcgat cgtcctcttt ttatatgtat tgaattactt
      481 agatatcaat ccattgattt tttctcgata gataagacac attcagcgga acagttcctg
      541 cttatcagca gaaaactgtt ctgctttttc atttttcatt gatctattct tcttttattt
      601 tcctagcatt tcatttgtga tgtatgcaat aacatgttat cttttattat atgaatcgtt
      661 gaggagtccg ttacatgatt acgttggtga gagttttgtt ttgggtgcct gcggtcgcac
      721 tggtcgcaag tattgtttat cttatgaatt ggaacaaaga acggttttat ttagctattt
      781 tgactcttcc ggctatctac tttatgtgga aagtttttaa ttacaactac tttgaacccg
      841 atagtgtttt tatagaagaa ttatcaggac tggttttgtc tttattgatc gtcattcttt
      901 atttgatacg tttaaataaa aaacattgag tactattgat tgcatgtgta gctttctata
      961 gtcattataa ctattctact ataatgaaag gtgattaaat gagtatcgaa gaagctattt
     1021 tactggatac tttacgttgt cgtctgaatt tcttaagaac tggccgatct ttatctcaag
     1081 tagctgtttc tagcgcgtta agtattcctt tattcaaata tcgtaagtac gaagaaggag
     1141 aagaaattcc aagcatcatt gaattaatct atatcgctga cttttacgat atttccgttg
     1201 attacctaat tggtcgctca gaaacagcaa gcaaactgaa gtgatttttt ccagccttgg
     1261 tgctggtttt ttcatttaac gtctaaggtt aacaatctat atataaagga gacatttcta
     1321 tgcgattatg gcatgaaaaa ttgatttcaa agcttcctcg tcaacagctt ttaggacaac
     1381 atcgggaatg ctgtgcatta agaggaaatg gctggggaaa gaaacatgct actgtggatt
     1441 atgtttttca ctattctcct tatagattat tccaatatca tcagcttgtc atggatgaaa
     1501 tggaaaaacg tggttacagc ccagcaccag agtggaaaaa tcctatgtat cgtggcaaat
     1561 catgcgaagc attttttgat ttacctactg tagagcgcaa cgtgccgatt tatccggaac
     1621 acgatgaagt ttatctagca gaatgtttag caaatttgga gcaaaaagga atttatctta
     1681 cctaatataa ctagaaaagc cttctatctc ctcttacttc attaaaaagc tttaaccatc
     1741 ttagatcaag ctaaaatgtc gtaagtttta gggttatctt catcctcacg tgcaaaacta
     1801 aaagcgtact ctagttttgg ttcatcgcca acttccctag acataaatga acatctcctg
     1861 acgtggttta aaagtaccgc atcttcgacg tctgtatacg atgcacagca cagattgctt
     1921 cttttgatct ttcttttgat ttttctgttg cttcttctac accttcttcg attactacat
     1981 cgatgcttac aattgcttct gcctaatatt cccatgcatc gtcgatttaa gcctctgtag
     2041 tcactgtgat cgtttccaca ttttaactcc tctgtcgtgg cttcagttga ttttgtgatc
     2101 agttcagttg ctaagccaga gtcagaacaa cctgccattg ctagcgttaa tattgatagt
     2161 aaaaatagtt tttttatcat aaggttcccc ccctgaaaat agactaaaat aataacgctt
     2221 tctttttttg ttttcagacg aaaattaata atttctaaat attctttaaa aaagcttata
     2281 actattatgc cttttttaat atataacaca gttattttta aaaataagaa tgctaaatca
     2341 ctctacaata acggcgaaag cacaaaaaat aaaaaaatta ttacgttatt aaaaaaacag
     2401 atgaaagatc aggttttatt agatctcatt ctttcatcac ttgaagggat ttaatgaact
     2461 actttttcct ctttgtcgtt tttatatatt cgtattcctt caaacagtag gatagcctct
     2521 ttcaatgctt gatatttgaa ctgtccgtta cttaaaaatt tagatgtgcc gccctcttcc
     2581 tagaacatat tcttttttac tttttcatta attgacagac caatcgaacg aaactgtaaa
     2641 tttcgatagg ataatgattg gtcaaactca ttcattttca agacatctcg attcatattc
     2701 aagataggaa tctttttatc aacatcactt tattcgtatt ttcctctacc ataaaagctt
     2761 cacgctccac aaaattgttt tatacactac tattcagccc attcaacgac caatgaat
//
LOCUS       DAYYCW010000189         2791 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000189 DAYYCW010000000
VERSION     DAYYCW010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.195"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..368
                     /locus_tag="ACHAYA_002908"
     CDS             <1..368
                     /locus_tag="ACHAYA_002908"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606633.1"
                     /translation="FKTAVFNSNQSVLLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVE
                     PVLEQGIKDKSLEIENSHEIAEVIMVLSNVWLNPLVEMTDKEGMEKRCNTFNALLNGI
                     GIDTLLDEETISAFIQFCK"
     gene            475..1713
                     /gene="mef(H)"
                     /locus_tag="ACHAYA_002909"
                     /gene_synonym="mefH"
     CDS             475..1713
                     /gene="mef(H)"
                     /locus_tag="ACHAYA_002909"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8606634.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            1940..2359
                     /locus_tag="ACHAYA_002910"
     CDS             1940..2359
                     /locus_tag="ACHAYA_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8606635.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            2364..2603
                     /locus_tag="ACHAYA_002911"
     CDS             2364..2603
                     /locus_tag="ACHAYA_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8606636.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT          902 a          388 c          513 g          988 t
ORIGIN      
        1 tatttaagac agcggtattc aattctaacc aaagcgtttt attgacagtt tctcctcaac
       61 tattaaacaa tcctcgtttt cttgctatgc aaatagaaca aatgtatgaa attgtagtac
      121 ctgaatttgt tgaacccgtt ttagaacaag gtatcaagga taagtctttg gagattgaaa
      181 attcacatga aattgcagaa gttatcatgg tattaagtaa tgtatggctc aacccattag
      241 ttgaaatgac ggataaagag ggtatggaaa aacgttgcaa tacattcaat gcccttttaa
      301 atggaatagg catagacaca ttattagacg aagaaacaat ttctgcattt atacagtttt
      361 gtaaataact gtatttgtta tccctatacg atagactgct ctataaaaga gcagtttatt
      421 taaaccatta tataaaccga cggtctgttt gattaaataa aaaggagaga ttttatgcaa
      481 gcagaaaaaa aactttttaa tcgtgatttt atacttgttg ttataggaca gataatttct
      541 ttatttggaa acgctatcct acgatttgca ttaccgtact atttgttaaa agaaactggt
      601 tcatcagctt tgtttggtat tgctacagcc tgctcttttt tacctatggt tattttgtcg
      661 ctggtaggtg gtattattgc tgacaggaca aataaaagaa acattatggt catactagat
      721 tttgtaacag caatcataat tttagtattt tatttgtcat tgggaaaaat tcctatcata
      781 cctttgttta ttgttgcttt gatgttactt tatggtattt ctggtgcata tcaaccagcg
      841 gtacaagcaa gtattcctct attggtatca tcagataaac ttaccgtcgg taatgctata
      901 ataaatcaag taaatacttt agcaaacttt atcggaccag ctataagcgg agtagttttt
      961 caattttatg gtatcaatcc cattttaatt gttagtatta tatgttttac tttttcagcc
     1021 attatggaaa tttttatcca tattccacac caaaaaagac ctcgtgaagt aggtgtaatt
     1081 gcagttgcta aaaacgattt aaaagatagt tatacctttt tgaaaaagga gaaacccatt
     1141 tttatatccg taatcttttt ggtttgcatt ttcaatttgg ttttaagtgc tgttttaatt
     1201 gttggtctgc ctatcttaat ttctgaagtt ttaaaaatgc cagatacact atatggtttt
     1261 tcacaatcag cattatcatt aggtggttta tgtggtggta ttttaacagc tcttgttgct
     1321 tcaaagttaa aactaaaaaa cctttatacc ttgttactta tttgttctgt ttctgtttta
     1381 cttatgggaa tttcgctatt tttagaattg cctgcaatag tttcctactg ggtagttaca
     1441 gtgatgtgct ttgtggctat gggtgtatct actatgtttg ttgttcaaat ttacactctt
     1501 gttcagacac aaacaccacc gcaattagta ggaaagataa tggctacgct ggtagcagtt
     1561 gctatgtgcg gtcaacctat tggacaaatg gtttacggta ttttattcga tgtatttata
     1621 caagaaacat gggttgttat gtttgtagct tctattgctt caatcatgat ttcaatatat
     1681 tcaaaaagaa tttttcaaaa gttagagata taatacaagg ggttaaatag tatgttaggc
     1741 taaaaacaat atataaaaga aaatttgaaa tctgctaaaa ggatagtgtt ataaaagaaa
     1801 gagtaatatt gcagtattta aggctcgcac aaatatatgt tttgtgtggg cttttttctt
     1861 accatttttc tgtatttcta aagggtataa acggtattcc gtttatatat ttcagcatta
     1921 gcagaaaggg ggtgagatta tgaggacatc ttctttcgag catatcgtca gattacagtt
     1981 caattcttta atgctgattg tcattaagaa caaagtgaaa agtcgaaaca gacagcttgc
     2041 aagacattct aaacgtgaag tattgttttg tgaaatagcc gaaacgaaac aagttgaacg
     2101 tggaacgatt gatacctgtt tttatgattg tgtatcattt aaagtcttgc actttacaat
     2161 ttatgtatct gatgaaacac ttggtacagt tttacataga ctgtcagaaa aacagcgtag
     2221 tgcgatttta ttacgttatt ttcaaggaat gaacgacaga gaaatttcag aactgtacca
     2281 tgtatcacgt tctgctattt gcagtagaag aactagagga ctaaggaagt taaaaatgct
     2341 tttgagcgaa aggaagtaaa gaaatgagta aagattatgg ctatccctct tttgaattga
     2401 tttgcaaagc gtcaagcgga gatgaaatgg cagttaaaga gattttgaag ttttatgacg
     2461 gttatatttc taaattatgc ttgcgtcctt tttaccactc tgaaagtggt aaaatcatta
     2521 tgcaggttga tgaagaacta aaaggagaaa ttcatacaga catgatgaaa gcaatattga
     2581 aatttgaaat cggagtgaag taatcaactg tataaaaaaa tcgggagata cacagtttcg
     2641 tgctatctcc cttagtagtt ttacggtcat atcaaggctc attcaatcgt ggtaaattcg
     2701 tggtaagatg agtgtttttc tatacttcaa aatgcttgaa tgtatagtgt ttatgcggat
     2761 aattgaaaat tagatataaa atttcaaata a
//
LOCUS       DAYYCW010000190         2675 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000190 DAYYCW010000000
VERSION     DAYYCW010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.197"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            699..785
                     /locus_tag="ACHAYA_002912"
     CDS             699..785
                     /locus_tag="ACHAYA_002912"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8606637.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1087..1311)
                     /locus_tag="ACHAYA_002913"
     CDS             complement(1087..1311)
                     /locus_tag="ACHAYA_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606638.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1677..1961
                     /locus_tag="ACHAYA_002914"
     CDS             1677..1961
                     /locus_tag="ACHAYA_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606639.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1964..2380
                     /locus_tag="ACHAYA_002915"
     CDS             1964..2380
                     /locus_tag="ACHAYA_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606640.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2517..>2675)
                     /locus_tag="ACHAYA_002916"
     CDS             complement(2517..>2675)
                     /locus_tag="ACHAYA_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8606641.1"
                     /translation="NPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKI
                     SLRNWLEE"
BASE COUNT          882 a          497 c          430 g          866 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tactcagata aaaagttgtt agtacccttc tgtatccgtt gggagagtaa
      241 ggctcaactg ccccctaaaa agggttctaa aaaatcttta aaaaataaat aaaataaacg
      301 cgcgcgcgcg agcaaaattt cccgccaaaa aatcacccac tttttaaaat aaacaggttc
      361 gcgcatgata ttaaactgcc aatctagctc cctatggtcg ctgtaagaag gagtgatcgc
      421 tacgctctca cattttactt gcagcagtcg cctttaggct ccttcgccaa aaataattca
      481 ttagggactc ctttgtggcg tcctgagagc tcgtctaagc cgttctaaga caagattcga
      541 taaaaactat gtccttcgtt ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac
      601 cccaaggcaa taaaaaactt aggcttattt ttgctttttt attgtataat tgattgtcgc
      661 ctatccgaca gtcttgcgac tggaaggagg tgtgtttcgt gttcactaac tttatttgct
      721 cccttcttgt tgggctttta agcgctttgt tcagttactg gttgaataac cgaaacaaga
      781 agtaaaactt aattaaggcg actcagccca caaaaaaagc cccagagaat ccgacctctc
      841 tggggctttc gtgtgtttcg tgttcactta tttgcaatca aattataaca gaaataaata
      901 tttatttcaa aaatatccta caaaagtcta aaatccgtaa ttatttctta tatgcaacat
      961 ttacaaataa agtcgctctg agagctcgta tttttcgatt taagacgttt tatttagttt
     1021 ttggtgtatt tcattggtcc aagctaaaaa actcttagaa gcgattttag agccatttaa
     1081 acccacttaa ttatattctc catgaaattc gctccatgcg tcaaaagctg cgtctgaaat
     1141 tccgccgtct tcgttcacta gttgcattaa tttttccaac gatcgttttc tttcgggtgt
     1201 catattttta tattccaaca atttttcgtc tgttaaatcg aacttgattg cttgaattgc
     1261 ttgctctaat ttttctaagt tttgaaaata ttcgtaagga aatttttcca ttttttcttt
     1321 ctccagtctc acaatttttc tagaaataaa atcacttctt tgccgtctat actctcaaaa
     1381 aaaatgtcgt ttgaaaaact aaataaattt ttgatcgtgc atttttaaat ttggttatcg
     1441 attacacgtg cagcactccc ccccgaattt taaagagcat aaggaaacgt gtacccactt
     1501 tctaaaaaaa gtgggtagat aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat
     1561 cactttttcg ccatcgtggg tagcttatgt atagcatttt ggcaaaaaac agctatacaa
     1621 atatagtatt tgtatggtat acataaatta tacataattg caaaaggaga ttttttatgg
     1681 acattttagt tcggaatatt gatccaaagt ttgttgcaac aatagacaaa cgttgccaag
     1741 aactcacgaa taaaacagga gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg
     1801 aaagtgattt tgaccgtcaa cttacgactt acaaaaaaga ccaattcgat ttattgctcg
     1861 ataaatttat tgctatccaa acagaaaata ctcaaatatt aagcgagtat gtctacacaa
     1921 ataatcaaat cattgaaatg cttatcggag gaatgatata accatgagaa aaacttctgt
     1981 ccaaaaacat gttactttgt accctagtca tgttgacgtt ttagattcca tcatcgaaaa
     2041 aaatccagat atcaataact actcggaagc cattcgcttt ttatgtatga gttatgaacc
     2101 cgataccaac acgaccacta ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat
     2161 ttccatcatg actgaaatca tcgcagaatt tgctgattta tatctaaaag atacaggcgt
     2221 tcttattggc acaaattcta aagtatatca agaagtgaaa gaaaaaatcg aagctaaact
     2281 aaatccatca aatcgaaatc gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac
     2341 ttctagaaat ttcgacccaa caaattttct aaaacgataa aaaacagata ggagttattc
     2401 ctatctgttt tttatttaga agccccatac aatatgctag aatgtactag caatcttagt
     2461 cggttgtaag ggacttgtct acttgcttaa tgtaaggggg gcgagttctt ttttatttat
     2521 tcttctaacc agttacgcag agaaatcttt ggcaaatctg acgttaggct tgaaaaagta
     2581 aattctaatt tggcgatttt attgcccttt tttgctttta tttttgtgac tttaagatca
     2641 ttgaagtatt tttttagttc tcgtaatgca ggatt
//
LOCUS       DAYYCW010000191         2674 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000191 DAYYCW010000000
VERSION     DAYYCW010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.198"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..512
                     /locus_tag="ACHAYA_002917"
     CDS             168..512
                     /locus_tag="ACHAYA_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM8606642.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            513..932
                     /locus_tag="ACHAYA_002918"
     CDS             513..932
                     /locus_tag="ACHAYA_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFM8606643.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1024..1374)
                     /locus_tag="ACHAYA_002919"
     CDS             complement(1024..1374)
                     /locus_tag="ACHAYA_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606644.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1340..2671)
                     /locus_tag="ACHAYA_002920"
     CDS             complement(1340..2671)
                     /locus_tag="ACHAYA_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFM8606645.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
BASE COUNT          996 a          485 c          518 g          675 t
ORIGIN      
        1 aaaaagcgtt agcaactatg cttaaaaagc atatcgactg aataaactaa gcattggaca
       61 ggctgaaaga ctacatttaa aatgaaatgc gaagaaacaa tagaaaataa agttgcctta
      121 gaaccatagt tgaatcacct aacagtcgtg aagggagaat aagagaaatg gcagaaaaac
      181 aaaccgcagg acgagatcaa ttaggagatt ttgcaccaaa atttgcagaa ttaaatgatg
      241 atgttttatt tggacaagta tggtccagag aagaattatc tgctcatcag cgtagtatca
      301 tcaccatatc tgcattaatt tcaggaggaa attttgaaca attgccagct catctgaaga
      361 ttggcaagca aaatggcatt actagtgaag aaatagcaga aatcattacc catttagctt
      421 tttatgtaga atggccaaaa gcttggtcag cttttagttt agcaaaagaa atctatttag
      481 aagaaaatag cgaagataag aaagaggcgt aaatgatgac aaaacacgaa gaaatacaca
      541 atggcgtgat ttttccagca ggtgaaaaaa acgaagctta cgctcaagta tttaccgggc
      601 aaagctatct gaatatgtta gtgaatgatc ccgaagtttc tgttggtgta ggtaatgtaa
      661 cttttgaacc aggttgtcgc aatcactggc atgtccatac agacggctat caaatcttac
      721 ttgtgaccgg aggagaaggc tggtatcagg aagaaggaaa gccagcaaga cgattaatgg
      781 ctggagatgt cgtcgtaacg cacaaaggga ttaaacattg gcatggtgcg acaaaagaca
      841 gttggtttga acatttggcg atcactgctg gtaattcaga gtttttagaa cctgtaacag
      901 atgaagtgta tgaaggattg gaaaatcaat aatgtcaagc ttgaacagct cctgaaagta
      961 gattttaatc gaactttcag gagctgcttt tttgattcag tcttttataa atagaaaata
     1021 gggttaataa taggaatgag aagcacgtcg aaagatctta atattgataa tcggctgggc
     1081 atacatgatt tccacgtcat caatcaaact acctaacaga tgataatctt cccgatcatg
     1141 tcggggatta cctaatagtt cttgatagta gatttccata ctagggtccc gaggattgtt
     1201 cagcaaatca gcaagtacat ctaaatcttc tggctgagtt tctgccgtac cctgtatctc
     1261 tatgaaaagc ccttcctcag caaattctac tccaatacgt atttcagtta tttcatggga
     1321 atataaataa gtaagaagct catcgatgat tctttttgat aattcttttt ccaagtgtct
     1381 ttttttcctt tctgacagcg gtaaagttat ctagtatcgg tacaaggata cctgcaacga
     1441 aaccagttga aaaaccactg ttatatagat ttagtcctcc gtgcaaagtg ctgacgttat
     1501 ggacaagagc aatatgtagc acaccagcta ttacaccgta aaaactgccg taaaaaccgc
     1561 ttatgggcgc aagacctgtc ccaaacacag ctgcaacaag catgccagta ctggtgatct
     1621 catgaaaagt tccaaacaaa gaagcaataa atattccgac tagtatcgga aaactgtttt
     1681 ttaaatgaca accaaaagca ctgaaaccca ccgcagataa aatagcaccg atgacaggtc
     1741 catttaactg accgccaacc agcaaaacat aagaagacag catcagacca actaagccca
     1801 tgttgatcaa agtagcacca ataccagaat tagcaatgaa atcagttgtc aattttccag
     1861 atgaatcgaa aatttctcga atccccgaaa gccgaaagga attataatag aaaccaattc
     1921 cgactgtcag cgaaaaaaag ccaaaaataa tccaaatgag aggaaagtga tattcattga
     1981 aaaccaatgt attttcgacg attgaatagc tgaataacct caaaacagca gtaaaagtca
     2041 tcgcaatcaa cccagaggtg aatccaatat tataaagtgt gaatccacga tggaaaacta
     2101 aagtttgagc agctaagggc ggaagaagta taccaattgc tatgccagct aaattcccca
     2161 ataaaatacc tatcagtaaa ggaaaacgca taccaaaagt gatataacta acgaccggtg
     2221 ataatacgct accaaacagt ccgatcatga tgtactgaga aaaaggttta ttgacaaata
     2281 cagcgtatag atacacacct aaaatgattg agatagaatt ataaaggttt ttgccaaaaa
     2341 aagagaaacc ggtaactgtt agaatcgaag caaataaggg accgtttaaa atcaccttgt
     2401 gccgataagc taaaagcaca gaaagtaagg tcaaaatccc tacatttatg aatgcgcttc
     2461 cgaaaacacc taaagcaaaa taatctgtca aaagattgct tggcgaggta agaatagtta
     2521 gcaatccact ccaattatca gtaggagtat tagaaagcaa gccaataaaa agaaaaaaaa
     2581 cacctgtaaa accaagataa aaatactttc ttttctgtga aacgattcgt ttttgaggat
     2641 gaatataaat aacgtcagtt tcgttttcca ttac
//
LOCUS       DAYYCW010000192         2581 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000192 DAYYCW010000000
VERSION     DAYYCW010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.199"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2260
                     /locus_tag="ACHAYA_002921"
     CDS             <1..2260
                     /locus_tag="ACHAYA_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFM8606646.1"
                     /translation="IDKLDTKDIVNWLNQDEELTDLLSSVKKESSASQQSNWSGLYFN
                     SNEYVKYILNKTAWIKIDGEKYSPRQIVLYEKLQAHVPGLYGVSEQSLMELLGQRIVE
                     QLDFRKNMAMFSDDEIRAILVELPKFDKGEISRRLYNELIRNKKGMNPTYSTEGLSVL
                     AKDGFFYPNKELRYADKRLPKSIEKQMRFIYVPEKASTTTIYEWLGVERFKTNLKLET
                     FELLEDYSEDFKKEIDDIKTAVLSTIDDNSKNVSSLKRIQIIPCSKISAKDVEQDGQS
                     IVLEDYYFVESKGKFYLKLPANSIEINQLRLIDTFSSTIVDIFKQVLNLSLDLNLIEL
                     LISRDTEHKQRKITEEFGVDKWNESFELLFSENALNKKVNDFLLSNGLNEEEYPTIFE
                     IDYSYALKEEEYQLLAIALESISKDIEDLNGLSELIHLDIREFWKLKLRSYLDKKAEH
                     YRQILYSNANKSGDEILKETFLEKIDRYKDYQFEISEIPNSVQVDIEKESKKRFPELS
                     VSITDEIDIDGLYDNNVEELVTVAAITIDDFDYYIRGHQKVSSLLYFGIPSRLREDIL
                     IFVTKENDVDLNGEKSGISIEGTSTVQTILEPSNSVSRKGKNTRNGERSKRDYDNRNS
                     ANDDAGETAEQIAYAELTQNQKLNVIWNTKYSSKSSDRNKQPPNGIVCDMWVHDPENG
                     NMYFEVKSSITEFEMSINEYNSMLDNPDSYEVVLVNRDTKEISRHKFDELDGLKQASS
                     YLFKFKQKRSDG"
     gene            2395..2523
                     /locus_tag="ACHAYA_002922"
     CDS             2395..2523
                     /locus_tag="ACHAYA_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8606647.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT          987 a          339 c          495 g          760 t
ORIGIN      
        1 aatagacaaa ttagatacga aagacattgt caattggctc aatcaagacg aagaattgac
       61 agatttatta tcttcggtaa aaaaagaatc aagtgcaagt cagcaatcga actggagtgg
      121 attgtacttt aattcaaatg agtatgtgaa atacattttg aataaaactg catggattaa
      181 gattgatgga gaaaaatata gcccgagaca aattgtgcta tatgaaaagc tccaggcaca
      241 cgttccggga ttgtatggag tttctgaaca aagcttaatg gaactcttgg ggcaacgcat
      301 tgttgaacag ttggatttca gaaaaaatat ggcgatgttt tcggatgatg aaataagagc
      361 tatattagtt gagttaccaa aattcgataa aggcgaaata agtcgcagat tatataatga
      421 acttataaga aataaaaaag gaatgaatcc tacttattct accgaaggat tatcagtctt
      481 agccaaagat ggcttctttt atccaaacaa agaattaagg tatgctgata agcgactgcc
      541 taaatcaata gaaaaacaaa tgcgttttat ttacgtgcct gaaaaagcta gtacaactac
      601 gatatatgaa tggcttgggg ttgaacgctt caaaacaaat ttaaaattgg aaacatttga
      661 gttattggaa gattattctg aggatttcaa aaaagagatt gatgatatca aaactgcggt
      721 attatctacg atagatgaca atagtaagaa tgttagttca ttaaaacgta ttcagattat
      781 tccatgcagc aaaatatctg ctaaagatgt tgagcaagat ggtcagtcaa tcgttttgga
      841 agattattat tttgtggaaa gtaaaggaaa attctactta aaattacctg ctaattctat
      901 tgaaattaat caattacgtt tgattgatac gttttcttca acaatcgtag acatattcaa
      961 gcaggtgtta aatctaagct tagatttgaa tttgattgaa ttgttaattt cccgagatac
     1021 ggaacataaa caaagaaaaa taactgaaga attcggtgta gataagtgga atgaatcgtt
     1081 tgagctattg tttagcgaaa atgcgttaaa taaaaaagtt aatgactttt tgttatcaaa
     1141 tggactaaat gaagaggaat atcctaccat ttttgaaatt gactattcat atgcattaaa
     1201 agaagaagag tatcaattgt tggcgatagc tctagaaagc atttcaaaag atattgagga
     1261 tttgaatgga ttgtcagaat tgattcattt ggatattcgt gaattttgga aactgaaatt
     1321 aagatcttat ctagataaga aagcggaaca ttaccgccag attttatata gcaatgcaaa
     1381 taaaagcggt gatgaaatac tcaaagaaac attcctagaa aaaattgata gatacaaaga
     1441 ttatcaattt gagatttctg aaattccaaa ttcagttcaa gtcgacattg agaaagaaag
     1501 taagaagaga tttccagagc taagtgtgtc aattactgat gaaattgaca ttgatggcct
     1561 gtatgataat aatgtcgaag agttagtcac ggttgcagct attacaatag atgattttga
     1621 ttattatatt agaggtcatc aaaaagtttc ttccttactg tattttggta ttccgtcccg
     1681 tttaagagaa gatatactga tattcgttac taaagaaaat gatgttgatt tgaacggaga
     1741 aaaatctggt atctctattg aaggaacatc aactgttcaa accattcttg aaccatcgaa
     1801 tagtgttagt aggaaaggta agaacacacg aaatggagaa agaagtaaac gagattatga
     1861 taatagaaac tccgcaaatg atgatgctgg tgaaacagca gaacaaattg cttacgctga
     1921 attgactcag aatcaaaaac ttaatgtaat ttggaatacg aaatattcta gtaaatcttc
     1981 agatcgtaat aagcagccac caaacggaat tgtttgtgat atgtgggttc atgacccaga
     2041 gaatgggaat atgtactttg aggtaaaatc atcaattaca gaatttgaaa tgtcgattaa
     2101 tgaatataat tctatgttag ataaccctga ctcttacgag gtcgttttag tcaatcgtga
     2161 taccaaagag ataagtcgtc ataaatttga tgaacttgat ggattgaaac aggcaagtag
     2221 ctatcttttc aaatttaagc aaaagagaag tgacggttga gtgatttgtt tccacaaacc
     2281 tattcaatct atcaactcaa aaatcataaa caaccattat tatagtagga agatacagag
     2341 tgtactctca acgcatatcg aattagtcgc ttcattcgtt aaaggagaat aaaaatggac
     2401 caaaaagaaa aaatgaaaga attgatcaaa aagaaacaag gtggaggccg atccaaacaa
     2461 atggaaacac ctaagaatga tcgtaaaaac atgagaaaag gccctaaaat ctttaataaa
     2521 tagacgtgct aaagaaagac taagaagtgt taacttttgt tattgtttct tagtcttttt
     2581 a
//
LOCUS       DAYYCW010000193         2436 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000193 DAYYCW010000000
VERSION     DAYYCW010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.200"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..2286
                     /locus_tag="ACHAYA_002923"
     CDS             133..2286
                     /locus_tag="ACHAYA_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM8606648.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          832 a          427 c          539 g          638 t
ORIGIN      
        1 tgctgtgaaa caaaaagttg attttatctt gaaattaggt aaagaatttc ttgctccgcc
       61 cattttttct tttttattaa gaagtttttg ggtacaatga agcatgaatg taaataggcg
      121 gaaggatagg aaatgatgaa agattttatg aaaaaaatat taaatacttc ttggttagaa
      181 aaaataagaa aaggtgcaca agaacctaag aacaaaacgt ttcgccggtc ccatgttcct
      241 tttcgcttaa attttctttt tttcattata tttacgttat ttgttgcgtt gattgctaga
      301 ttgggttatt tgcagatcgt gaacggcgaa gagatggagg cgcgagtgaa gtctacttct
      361 acgattacgg tccaagaaag tactcctcgc ggaatgatct acgactccac aggaaaagct
      421 ttagttacga ataaagcgaa tcaggccatc acatatacga gaggaacaca agttacggct
      481 aaagatttgc ttgaaatcgc aacgaagctg aactcgctga tcgatgttcc ggtagatgaa
      541 aatctgacag atagagataa aaaagatttt tggctggctg atcccaaaca tttgaaagaa
      601 gccacaaaaa gactttcggc aaaagaaaag caattaggga acagcgaaca atatagtgcg
      661 atggtcaata aagtcacagc tgatgagatt aatttaaacg aggagcaatt aaaaattgct
      721 accatcttta aacggatgaa tagtgcatat gaattaaaca ctgtctatat caaaaactct
      781 ggtgttactg atcaagagtt agctgttgtg gcagaacgcg cttctgaact tccaggagtt
      841 tcgactggca cagactggac acgagaatat aatgcagcaa gttcactgaa aagtattctt
      901 ggcagtgtga cgactgaaaa gcaaggactt ccagcagatg aagcagagaa atacctagca
      961 aaaggttatt ctcggaatga ccgtgtagga caaagttatt tggaaaaaca atatgaagat
     1021 gtcctgcaag gaaccaaaac ccaatatgaa gtttcccttg ataatgaagg aaatgtttcg
     1081 aatcaaaaag aaatcttctc tggggaaaaa ggttctaatt taatgttatc tatgaatgca
     1141 gaattccaat caaaagtaga agaaattttg aaaaggaatt atcaaacatt gatcaacaac
     1201 ggcaaagcac agtattcgcc tggtgcttat gcagtggcta tgaatcctca gacaggagaa
     1261 gtgctagcaa tgactggttt cagtcatgaa caaggttcca aagaaatcac cgagaatgct
     1321 ttaggcacga ttactagcgc ctttgatcca gggtctgtag tcaaggctgg tactttgaca
     1381 gcaggctggg caagtaacgc gatcagcgga aatcaagttt tgattgatga gccgattcgt
     1441 ctgcaaggtg cttcagaaaa aagctctgtc tttaatcgaa gcggacaagt tgctcttgat
     1501 gcagtcaaag catcggaatt gtcttctaat acgtatatga tcaaagtggc gttaaagatg
     1561 ttaggactag actatacacc tggaatggga ctacctagtt tagatgaaga agctaaagct
     1621 tatcagcaat tgagagactc tttcaaagag tttggtcttg gtacaacgac tgggatcgat
     1681 ttgccgaatg aatctcctgg gatctcacgt tctgttgatt atatgaagaa gttcaatgcg
     1741 gataatggca aagaatggta tgctccaggt aactttaccg atttggcatt tggacagttt
     1801 gacacatata caccaatcca attagctcaa tatgcttcaa cagtcgctaa tggcgggaaa
     1861 cgagtacagc cgcacctagt caaagcgatc tatggcaatg acgaaaatgg aaacttgggt
     1921 gaagtgaaga aagaaattgg cacaacagta gaaaatacgg tcaatatttc tgcagaaaat
     1981 atggcgatcc tccgtgaagg attccaccaa gttgtccatg gtaccgatcc ttacacaaca
     2041 gccaaaccat tagccagtgc gaaaatggac ctttccgcaa aaacagggac tgcagaaacg
     2101 gttgcggaag gtcatccaga tattgcgaca gtcaatagta atatcgtggc atacggacca
     2161 acagacaatc cggaaattgc catcagtgtt gtcttgccga acttattaga cgaacaagac
     2221 cacataaact tgacgattgc taaagaaata atggacgcgt attatgatat gttcatggca
     2281 aaataaaaaa gaggtcgtga aaaagccgct tagtttcgag tatgaagaaa aattttgtga
     2341 aaatagcttc tcacattttt gcttaaattt gacttcatac cgagaaactg gcttttgaac
     2401 accgtttact cggcaaaaaa ggttgtgaca aaaatt
//
LOCUS       DAYYCW010000194         2398 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000194 DAYYCW010000000
VERSION     DAYYCW010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.201"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACHAYA_002924"
     CDS             207..2069
                     /locus_tag="ACHAYA_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8606649.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="ACHAYA_002925"
     CDS             2069..>2398
                     /locus_tag="ACHAYA_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8606650.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAYYCW010000195         2344 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000195 DAYYCW010000000
VERSION     DAYYCW010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.202"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..903)
                     /locus_tag="ACHAYA_002926"
                     /pseudo
     CDS             complement(296..903)
                     /locus_tag="ACHAYA_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1426..2100
                     /locus_tag="ACHAYA_002927"
                     /pseudo
     CDS             1426..2100
                     /locus_tag="ACHAYA_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            2093..2254
                     /locus_tag="ACHAYA_002928"
     CDS             2093..2254
                     /locus_tag="ACHAYA_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606651.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          835 a          377 c          409 g          723 t
ORIGIN      
        1 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
       61 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      121 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac
      181 tgttgccatt ccatctatga atataaatag agtgtatctt ctttgataaa tgtcagaaaa
      241 agtgtgacaa ataaccatca gtaaaattac ttgtcatact attgataagg agtatttaat
      301 tgaagagaat gattaggaga aaggatctcc gtacttttta tagtaatcat agaatccaag
      361 tttgtataga cgaatctttt tttctcttat ttcttctgtt tcaaaatgga ctgcttgaag
      421 aatcaagtct gtgaattcta taggagctgt tccattcgca gtaatcaaat tacgatcttt
      481 cacagcttgt tgttctataa aatcttcttt attctgataa ttttccgatt ctaaccaagg
      541 aaattgcgca tttccggtgt gtttgtaacc ttgcaataat ccgctctgag ctaaataatc
      601 aactgcccca caaatagcag ctactggtac gtctgttttt aacaatttat cgattatttg
      661 atataaatga tgatttttta tgtcccatga attgccaccg atcataataa aaaggtcaac
      721 tttttcaggt atttgttcga ttaagtaatc gatttttgtt tttaatcctc caatggaagt
      781 gacctctgat tggatagaag cagttttgtt ttgccatccc tctttttggt ttagtgtgct
      841 agtaatataa gcaccttccc aatcggcata ttcctctaat agaaacgtaa tcgcagtttt
      901 cattttcaag ctctccttta ctatctatga caaggacaac agttttaaca tacagacatt
      961 cattaaattt ggatagcgac agcgaaaaca acaattctta agttttagac agagtataca
     1021 gacagtcaac ttaattaaat acgaactaaa taaaaatgac aggtaatcat ttttgtttac
     1081 cagcattata acattttgca tacatggatg acaaaagaac caattcccac ttgcttgagt
     1141 acaatcttaa aagaaactgg aaggcaaaaa gtaattatag aaacgaaaaa taaatcatat
     1201 cgggcactca gacatgaaaa caaccctaga aatatataac tagtccaatg aaaaaaaagc
     1261 gaatgccgta gaaaacaagg attctgcgta cattcatatc tcattttgcg agcatatctg
     1321 ctattactgt gactaaatgt taatatggtt aaaatgaccg ttatattttt tatataaata
     1381 tcgtataatt agtatgaata tagaagaagg aaagaaggtg caatcttgga acctaaagac
     1441 aagtatttag attacgctag ggtcaataaa gataaattta ttgaaacaat catcaaggga
     1501 aaaatccctg atgatgaaaa agatgcggta ttcatggctg gaagcccagg agcaggtaaa
     1561 acagaggttg cactaggttt agcttaaaat tatgacaacc atgttgtgat tgatgcggac
     1621 tattttagag cacaatttcc agaatacaat ggaaagaact caagtgtttt tcagaaagct
     1681 tcttcttggt tagttgaaca atcattaaaa tatgtactgg aacatggcta ttctttcata
     1741 ctagaggata cttttgctat ccttagtgca gagaaaaata ttattcgagc ccttaaaaat
     1801 aactttcgcg ttacgatttt ctatgtttac caagatccca aagttgcctg ggatttcact
     1861 cgaaaacgtg aactagctga aggacgtcat gtgccaaaag aaatgtttat caatgcgttt
     1921 tttaagtcca gagagaatat tgaaaaagtc aaaaatagac atcctgaagt agtccttcac
     1981 ataatgatta aggattatca aaacgacatt tctgaagtgc attatgcagc agataatatt
     2041 caattagtac taccaataca atatacttca aaagagttgg aggaggaatt acatgactga
     2101 gaaagaacaa gtacaacaaa tcgtgaagaa gtataataaa agtatcgctg atttatcaga
     2161 aaacgcctca gcgaaagaat ttaaaacagt aatgaattac gtagcagatg aagcaaatcg
     2221 taagcagcga aaactagttg ggttggataa ataaattttt taaaccatat ccttgtaaag
     2281 ttgagttttt tattttaatg aattaagact attaaataga taattattgt aacttaaaaa
     2341 acac
//
LOCUS       DAYYCW010000196         2224 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000196 DAYYCW010000000
VERSION     DAYYCW010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.203"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..759)
                     /locus_tag="ACHAYA_002929"
     CDS             complement(<1..759)
                     /locus_tag="ACHAYA_002929"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HFM8606652.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYLPFFIVKINKKITGYVTILSLLLFSIGQSYISSIFINTKY
                     DAYFDRNSTSISHVVMFALFFSANLFLVHKLSKMN"
     gene            complement(731..1627)
                     /locus_tag="ACHAYA_002930"
     CDS             complement(731..1627)
                     /locus_tag="ACHAYA_002930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8606653.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLL
                     EEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFAN
                     SSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGEN
                     LKIGSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMNKRLLKFFIAIAFETD
                     KLSRFRAAIRGVRDGYRGVIGD"
     gene            complement(1617..>2224)
                     /locus_tag="ACHAYA_002931"
     CDS             complement(1617..>2224)
                     /locus_tag="ACHAYA_002931"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606654.1"
                     /translation="CSFNVDDQNDYGEKIVNKSLESTLNLAYLGTINNIVDLEAINEL
                     LKTCKRKFGNKINLYFIGKGESKTKLADICEKNHIEFVDCGVIYDNAEKKVILDKCHF
                     GLNLFKSNTIIGLSLKSIEYFRFGLPIISNLSGDTKEIVKRYNVGINYNSTVDENEML
                     KFYQCNKNINENVRKIYATIFSEQVLKMKWNEVYKVMNDEK"
BASE COUNT          767 a          380 c          266 g          811 t
ORIGIN      
        1 attcattttt gataatttat gaactaaaaa aagatttgct gaaaaaaaga gagcaaacat
       61 cactacgtga gaaatcgatg tactgtttct atcaaaatat gcatcatact tcgtatttat
      121 aaatattgaa ctgatataac tttgtccaat agagaatagg agcaagctaa ggattgtaac
      181 atatccagta atctttttat taatttttac aataaaaaaa gggagataaa aatatgcaag
      241 attatggaac agtccagcca ctaagataaa aaaaatatac ttaaatatag ctttgctgcc
      301 tcgcaattta gttagatgat aaaaagctag taatatgaat atcaatgcgc taaacatacg
      361 tacttgtatt gcttgtgaag taaaaccaaa aataaaaaac aagaacaaaa ctaaaaaaac
      421 attagttgtg aataatctga tgacatacaa taaagctgag agtaagacag tagaaataat
      481 caataaaaaa atctgatact gactggaaat aaaggtacat acgtacataa cagctctgaa
      541 tcccggttca atactatctg aactaaagtc cataccaaat tgtgctatcc cattatacaa
      601 gacagaatat gcataataat cagcgttttg tgtgttaaat gccatgatga tccacattat
      661 caaaagtagc accccagaca aaatcttgtt cgatgtactc catttttttt gtgaaacact
      721 cttttcttga ttaatctcca atgacgcctc tgtatccatc tcgaaccccc cttatagccg
      781 ctctaaatct actgagctta tcagtctcaa aagctattgc aataaaaaat ttcaataatc
      841 ttttattcat aaggcgggaa aattccttat cacttttata cattcttttc accaatgttg
      901 aatttcttgc aataaaatac tttcgtgttg aattatgatt gtctatttta attttttttg
      961 atcctatttt taaattttct ccatctccaa cgtgatgata aaaatagact tgatttgtta
     1021 cataaatgtt atagccatta tttgcaaccc ttttacaaaa ttcaacatca atctgatcaa
     1081 taaaaaattc atccttcata tttccaacat cgtcaattaa ttcacttttg aaaaacattc
     1141 cagaggaatt tgcaaatgct attttcatgg aaccactagt tgaagcaaag tgttttttta
     1201 cgtatctccc tctctcattt ttgcaggtaa aagagacaga ttcatcattg ctattctcaa
     1261 agtaattggg aacaataatc ccaatgtcct tatctttcgc ttcaatattg gtaaactctt
     1321 ctaataattt taaagaaaga ttcttttgta tgatggtatc ttgatctagt aaaaggatat
     1381 aatcacattt ctcttttttg gcaatttcaa tacctatgtt ttgagctgtc gcaagcccaa
     1441 tgttctcatt catttttttt aaaattaagt tttccatcat ttcaaattga aaagagttaa
     1501 tattatctga gccgttatcc acgataataa gtttgttctg tttctcttgt attaacgtat
     1561 ccactaataa ctttatgtta ttatctggat tgtaggtaac aataattatc ccaattttat
     1621 ttttcatcgt tcatcacctt gtacacttca ttccatttca ttttcaatac ttgctcagag
     1681 aatatagtgg cgtaaatttt tctaacattt tcatttatgt ttttattaca ctgataaaat
     1741 ttcaacatct cattttcatc aacggtacta ttataattga ttccgacatt atatctttta
     1801 actatttctt tcgtatctcc tgataaattt gaaattatag gtaatccaaa cctaaaatac
     1861 tcaatcgatt tcagacttaa accaattatg gtatttgact taaataaatt tagtccaaaa
     1921 tgacatttat cgagtataac ttttttttct gcattgtcat atataacacc gcagtcaaca
     1981 aattcaatat gatttttttc acaaatatca gctaatttag ttttgctttc accttttcca
     2041 ataaagtaaa gatttatttt gtttccaaat tttcttttgc atgttttcaa taattcatta
     2101 atcgcttcta aatctacgat attattaatc gtacctaaat aagcaaggtt caacgtagac
     2161 tctaagcttt tattgactat tttctctcca taatcatttt ggtcatctac attgaaagaa
     2221 catg
//
LOCUS       DAYYCW010000197         2189 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000197 DAYYCW010000000
VERSION     DAYYCW010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.204"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACHAYA_002932"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACHAYA_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(987..1952)
                     /locus_tag="ACHAYA_002933"
     CDS             complement(987..1952)
                     /locus_tag="ACHAYA_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8606655.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          629 a          506 c          346 g          708 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggctttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cgcgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgagggt tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cttaaacatt gacgtaaatt tactctcact tatctgactt ttttactcgg tatcactccg
      721 agtaagccgt catattcatt gggataatct cgtctgcttc gctctaattt tccagagtga
      781 ggggtaactc cttcccccga attaccccaa agagtgacac tgggatatcc ctaaatttat
      841 tataaaaagc caagtctctt tgcgcaactg ttttatccaa gtcggcattt gcggattcgt
      901 tagcgttttt tcgctcttgc tgcaaacgca cgacccacca tgccgacttg tgctgaaaat
      961 aaaaaagtgg acaaaaattc atccactcat tcttccgtca cgtccaaccc caaaagccac
     1021 gtatccaatt cttttttcaa aacatctgcc gattccgaaa atgtctctcg taaatgatta
     1081 actaactcat cactttggct ttcttttttc aacaaatttc gatatttata ttttgtatga
     1141 cgagccaagc cagcccataa attttgatct gaaatcaatc ctgcaatcat aattctatca
     1201 gtatgctttg tatcagtcgg caaaaaatga ggacttgcga aactgtctaa actttcatga
     1261 accatcgcat accaatcagt agcttttcca cgccgtaact gcaattccag tcgccaccaa
     1321 gtttttattt ccggtggtac aattttccgt ttggtttctt gttccaattt tttgttgtac
     1381 atccttattt gtcgctcact ggcacgacta ccccaatatg ccgtttccag cttcccagac
     1441 cgcccataga ttggtttgaa tgaaacaggg tcaaccacac gatattgcgt aataaaatca
     1501 tcaggcacat cgataatgtc gcaagcaata tcagcacgtg agaaatgtgg ttctaaaaat
     1561 aaatcatgga taaagttctt catcgaaccc gccaaaaatt ggctaatttt atttgggtta
     1621 aagtcaattc gcccttgccc ttcttgatat ttcaaaattt caatgtaagc aatattctca
     1681 tcatgcttat ccacaatttg ccaaccattt agaccaacag atttaacata accattattt
     1741 tcattcagtc gcattaactg ttcaaagtct aaaatcaata catcattttg cgtgtgataa
     1801 taaatattct ctttgagttt tccaacaatg gtaattcggt caattgacca ctgtatttta
     1861 aggggggtgg tcaaagtttg cacacttaac cgcctgttag atagggctgt taaattttcc
     1921 cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa gaaaaagaga
     1981 cttttggaaa gaatttccag agttcacgta accctaaaaa ttcatatcca aaaatctcta
     2041 gcttgaagaa acctaaaaat attcgtataa tgaacctgag ttacggagct aggtgaagta
     2101 gtttccgtaa atagtcgaga gtgttagcgc actgctcggc ttttattttg ctcttttttc
     2161 tcgttttctt tgattatagc actcctcct
//
LOCUS       DAYYCW010000198         2096 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000198 DAYYCW010000000
VERSION     DAYYCW010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.205"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>2096
                     /gene="acm"
                     /locus_tag="ACHAYA_002934"
     CDS             <1..>2096
                     /gene="acm"
                     /locus_tag="ACHAYA_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM8606656.1"
                     /translation="PVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEKKQN
                     IQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKNLDS
                     VEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTGDML
                     PEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYRGEE
                     GIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFENHSE
                     AWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTFELR
                     RADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELKFSI
                     NENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDAETK
                     RLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNENKE
                     VISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPKYRD
                     GQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTEREKI
                     RNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVIIGL
                     LIVTICAGIFLQKYKKV"
BASE COUNT          823 a          343 c          411 g          519 t
ORIGIN      
        1 cctgttcatg tctatgcgga tgcaggcaga gatatcagca gtaatgtcac ttcgttgacc
       61 gtggacccaa cgaatattac agatggagga aacataaaag taaaattctc gtttgacgaa
      121 aaaaaacaaa atatccaacc aggtgattat ctttggatca attggccaag tgaaggaaat
      181 attcgcggtg aaggattcca aaaagaaatc ccattaatga ttgaaaataa aaatgttgga
      241 actttaacgg tcagaaaaga ttctgcgcag gttgtgttta atgaaaatat taaaaaccta
      301 gactcagtag aaggttgggg gcaatttgag atacaggcca gaaacgtaac cgataccggt
      361 gaagaaaata cgggaccatt caccgtgacg agcggtgata aaacagctac tgtaaatgtg
      421 acaaagccag cttctggttc ttcttctagc gttttttatt ataaaacagg agacatgcta
      481 cctgaagata ctaagcacat tcgatggttt ttgaatatta acaataacgg cacttatgtt
      541 gaacaaccag ttaaaatcag tgatgaaatc caaagtggac aaagattaga tccaagtacg
      601 tttgaaatta atcaaataca cttaggtgaa cagaaggttt atagaggaga agagggaatc
      661 caacaatttt tacaagattt ccccagcgct acatttaatt tttcagtaac tgataattac
      721 attgaaataa ctataccaaa aaactttgtc aatcttagga aaataatggt gagttataag
      781 acgatcatag aaaacccaga acaaataaat tttgagaatc attcggaagc ttggtttaaa
      841 gaatttaata aacctgctgt tgatggagaa agtttcaatc atacagttaa aaatatttca
      901 gcttctggtg gagtcaacgg tacagttaga ggcgaattga agattttcaa atatatcaat
      961 gacacagaga ttggtatccc aaatgtgact ttcgaattaa gacgagcaga tgaacagcct
     1021 attcaaggac agtctagtat cctgttgact tcgaatgaac agggagaagc tagtatcaaa
     1081 ggcctgcaag taggagacta tgttgtaaaa gaaaaagaag cacccaattg gattgatttt
     1141 gatccactaa gttcaaatga actcaaattc tccataaacg aaaatgatac agagggagtc
     1201 agtttaccta tttataacaa gaaaaaggtt acgaacatca cagcaactaa aatttggaac
     1261 ggtggaacca ctccaagacc gagtatttat tttaaattat tcagagcaag tacaaacaat
     1321 tgggaaccag ttcctgatgc agaaacaaaa cgattagaca atggaataac ttcagtgact
     1381 tgggaagata tccaacagta tgatgatagt ggaaacgaat atacgtttaa agtgcaagaa
     1441 gtagatcaaa acggaaatga ttatgtccct tcaggttatc aaaaaattga aaacgggcta
     1501 gtcgttacaa atgagaataa agaagtcatt tcagtctcag gacaaaaaac atgggaagac
     1561 aatgagaacc aagacggcaa acgaccgacg acgatcacag taaatctatt agccgacggt
     1621 caaccgatcc aacacaaaga agtaagtgaa aaagatgatt ggcgctatcg tttcacaaat
     1681 ctgccaaaat acagagacgg gcaagaaatc atctatactg taacggaaga caacgtgccc
     1741 gaatacagta cgacgattga cgggtataac atcaaaaaca gttatacacc ggaaaaaaca
     1801 aatatatcta taccaggtag ccatataaca ccgtggaaaa attttccaga gaagacagag
     1861 agagagaaaa ttagaaatct attctctcaa aaattgcaaa tatttgctca tacaggagaa
     1921 tccagcgaac aaagaagcaa ttatattagt aagagagctg tacctaaaca gataacaaat
     1981 aaatcaaaaa gcattcttcc taaaacaagt agtaaaaaat ctagttttgc tgtaatcatt
     2041 ggtttattga tagtaactat ctgtgctggg atattcttac aaaaatataa aaaagt
//
LOCUS       DAYYCW010000199         2003 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000199 DAYYCW010000000
VERSION     DAYYCW010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.206"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..496
                     /locus_tag="ACHAYA_002935"
     CDS             <1..496
                     /locus_tag="ACHAYA_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8606657.1"
                     /translation="LNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIAVHP
                     SAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPGVCL
                     AGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKKEGI
                     NFEF"
     gene            447..1628
                     /locus_tag="ACHAYA_002936"
     CDS             447..1628
                     /locus_tag="ACHAYA_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8606658.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            1641..>2003
                     /locus_tag="ACHAYA_002937"
     CDS             1641..>2003
                     /locus_tag="ACHAYA_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8606659.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLK"
BASE COUNT          711 a          262 c          408 g          622 t
ORIGIN      
        1 attgaatagc tgggcggata gcaaaaaaaa aatctataaa tttattgtag ctattggtga
       61 ctgtgtagta agaaaaaaga ttataaaaaa attgaatatg ccaagtgagc aatatggaat
      121 agctgttcat ccaagtgcta tagtggcaaa agaaatttca attggttatg gaactgttat
      181 ttttgcgaat gctgtaatta acccagatgc tgtaattgga gagcatgcta taataaacac
      241 aggtagcatt gttgagcatg attgtagaat taataactat gttcatttat ctccaggtgt
      301 atgcttagcg ggtggagtac atgtaggtgt aggaactcaa ataggaattg gttctcaatg
      361 tatccagatg aagaaaattg gatcttggtg tatgataggc gcagggagca ctattgttaa
      421 ggatattccc tcacattcct tagcttatgg caatccagct aaaattaaga aagaaggaat
      481 taattttgaa ttctagaatt ttattatcaa gtcctcatat gggaggaaca gaagaaaaat
      541 atgttgctaa ggcttttaaa gataattgga tagcgccatt aggtgaaaat gtggataaat
      601 ttgaagaaag tattacaaaa tatactggtt ctaagggcgg agcttctgcg ttaagttcag
      661 gtacagctgc catacatctt gccttaagac tgcttaatgt tggaaaaaac gatattgtct
      721 ttgctccttc atttactttt atagctacag taaatcctat tttatatcaa ggggctgtac
      781 cagtgttcat tgactcagaa cctaatacat ggaatatgag ccctgttgca ctagagagcg
      841 cattagaaga agctgaaaga aacaaccaat tacctaaggc aatcatcgta gtgaatttat
      901 atggtcaaat ggctgattat gaacgaattt tagctattgc aaaccaattt aatgttccag
      961 tgatagaaga tgcagcggaa tcgttaggtt ctacttataa gggaatttcg tcaggcttat
     1021 ttgggacaat tgggatctat tcatttaacg gtaataagat catcacaacc tcaggtggag
     1081 gagctctagt gtctagtgat aaaaaatatg ttgagcattc aagattttta gcaacacaag
     1141 caagagatca agcaccgtat tatcaacata gcgaaatagg ttataattac cgtctaagca
     1201 atatttctgc cggtatcgga cgtggacaaa tggaagtatt gaatgataga gtagctaaac
     1261 gaagagaaat tttcaatcga tataaggaag ctttttctgg aattgataga attgaaatga
     1321 tgccagaagt ggatggcatg gtgccaaatc gttggttgtc tactttaacg atcaatgatt
     1381 ataattctat tgatcaagta gttaacgtaa taaataaact aaatgaaaaa aacattgaaa
     1441 caagagcttt gtggaaacca ctacatttgc aaccagtatt tcaaggggta aaaatgtatt
     1501 accatgataa taatcttagt gtgtcagaaa agttatttaa tagcggctta tgcttacctt
     1561 ctggaagtaa tatgacagat agtgaacaag aatttgttat tgatgagttt aaaaaaatat
     1621 tcaagtaggt gtgatatttt atgaacatag gaattattgg tttaggttcc atggggaaaa
     1681 gaagacttag actcattcaa gaacacttta atgatatttt actatgtggt gttgatacta
     1741 atgaggcgag acgaaaggaa gttgaaaagg aatttggaat accaacttat aatagtctaa
     1801 cagaggcaaa aaatttgttt gaaataaatt cagtttttgt ttgtacatcc cctataaccc
     1861 atgaatttat tataaaagag gctttaaatt tagattgtaa tatctttacg gaaatcaatt
     1921 tattaaatga atattatgat gaaataatcg aagtagctaa aaagaaagat agattacttt
     1981 atctatcttc tacatttttg aag
//
LOCUS       DAYYCW010000200         1994 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000200 DAYYCW010000000
VERSION     DAYYCW010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.207"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..401
                     /locus_tag="ACHAYA_002938"
     CDS             <1..401
                     /locus_tag="ACHAYA_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFM8606660.1"
                     /translation="SPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNS
                     EDEDKIKKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFI
                     KSAAQFIAADYDADLADQFINGNNFMPTTN"
     gene            complement(532..1008)
                     /locus_tag="ACHAYA_002939"
     CDS             complement(532..1008)
                     /locus_tag="ACHAYA_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8606661.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(968..1324)
                     /locus_tag="ACHAYA_002940"
     CDS             complement(968..1324)
                     /locus_tag="ACHAYA_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606662.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(1302..1487)
                     /locus_tag="ACHAYA_002941"
     CDS             complement(1302..1487)
                     /locus_tag="ACHAYA_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606663.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(1488..1760)
                     /locus_tag="ACHAYA_002942"
     CDS             complement(1488..1760)
                     /locus_tag="ACHAYA_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFM8606664.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(1757..>1994)
                     /locus_tag="ACHAYA_002943"
     CDS             complement(1757..>1994)
                     /locus_tag="ACHAYA_002943"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606665.1"
                     /translation="NTKIFSKRGGTVVKVREQFKIVSDEGNVYKIKIVNINEYREADH
                     KYLVDLYMNDKYLGEGFCGDSLFDDERILKEETE"
BASE COUNT          589 a          379 c          285 g          741 t
ORIGIN      
        1 tttctcctac atttgttgat atttatactc aagccacaca agctaaatct gagcaattgg
       61 aacaaattgc tggagttggt tatagaaaag ctctagaatt tctaattaaa gattatgcaa
      121 tatctaaaaa ttcagaggat gaagataaaa taaaaaaaat gttattaggt aaaacaattg
      181 aaaattattt gaatgatttt cctaaactcc aaagattagc caaagctgct acttggattg
      241 gtaatgatga aacgcactat gttcgtagac atactaataa agatgttcat gatatggatg
      301 catttattaa atcagcagcg caatttatcg cggctgatta tgatgctgat ttagctgatc
      361 aatttataaa tggaaataac tttatgccaa ctactaatta aaagaataaa agagccacag
      421 ataccaatta ataaaacggt tgggatatct tggctctttc atattttttt gacactaata
      481 gaatatcatg ctgctaacaa tgtttacgaa catttagtga acagtgtaat tttagaataa
      541 agagagttgt tctttgtact tctcgctatt gtaaaaatca atttcgtttt cttttttcaa
      601 tcggtcagct tgtctgtgtt catactcatc taaaaagttc aatgtctttc taatttctgc
      661 atgtcgctgt ctgacatacg atgagctata cccaacagat tcagcaatat cttctaagct
      721 catttgatca acatatttca tcttaacaat ttgattatcg ataccagaaa agctatctat
      781 cagcaataac atttcttctt tttgctcgat caacaattcc aattcatttt ctatcttttg
      841 gatattctct tctaaagaag aagttctcga attcttttcg atgcggacgt ttgctaaatc
      901 accacagacc catcgattca attcaagctt acttttatta agattccact ttaagtaaag
      961 aatctgctca tctagttctt ggtaatcttt taaccaatga aatctcacaa acgccacccc
     1021 ttatggtaga atagtattgc gctgctatct cgaaaggggt agctttttta ttttttctta
     1081 gccgttccat tctggattgc cttttgttcc aaacgtcgtt ttttcttctt aatctttgat
     1141 tttgtttttc ccatacaatt ctcctttccg aaaacacatt ttgttcgatt tttaatacta
     1201 aataaaagtt ggccagaatt tttcgttcgt ctttactcat ctttttttga ataagattac
     1261 tagcataaaa atagttagta ccacgaaaat gaacaggtaa atcaattacg ttcaccttct
     1321 tcatgccagt tatcagccac tagaatcttt gcgttagtgc cgccaccgtt ttcttttttg
     1381 tatttttcaa atgctttata agcagctgtt tttgatttat agatttttcc ggaaccgact
     1441 gggaacatcc atgtgccctc tgtgatagct aagatgtata ctttcattta attcacccct
     1501 ttaatcccca acactacata tccggacttc tgcatgtaat cagtaatata agtgatctct
     1561 gctttgtatg agtcaccagt gtaactaaat gttggtctcg ttagtccagg taattcatgt
     1621 ttaatgtatt ttttatattc acgtagtatt aactgatcac caactttgaa gtcacgatca
     1681 ttcttgcgga tctcgaattg tttgcgtccg ctcgtcactg cttcaaaata ttctggtagt
     1741 atttttagtt cgtggatcat tctgtttcct ccttcaaaat tcgctcatca tcaaaaagtg
     1801 aatctccaca aaatccttct cccaaatatt tatcgttcat gtataagtca accaaatatt
     1861 tgtgatctgc ttctcgatat tcattaatat taacgatctt tattttgtaa acattacctt
     1921 cgtcgctcac tatcttgaat tgttctctga ctttcaccac cgttcctcct ctttttgaaa
     1981 aaattttagt gttt
//
LOCUS       DAYYCW010000201         1941 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000201 DAYYCW010000000
VERSION     DAYYCW010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.208"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="ACHAYA_002944"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="ACHAYA_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8606666.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="ACHAYA_002945"
     CDS             853..1041
                     /locus_tag="ACHAYA_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8606667.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="ACHAYA_002946"
     CDS             1054..1611
                     /locus_tag="ACHAYA_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8606668.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="ACHAYA_002947"
     CDS             1643..1885
                     /locus_tag="ACHAYA_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8606669.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          715 a          299 c          400 g          527 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagcg a
//
LOCUS       DAYYCW010000202         1901 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000202 DAYYCW010000000
VERSION     DAYYCW010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.209"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..426)
                     /locus_tag="ACHAYA_002948"
     CDS             complement(<1..426)
                     /locus_tag="ACHAYA_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8606670.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(535..1566)
                     /locus_tag="ACHAYA_002949"
     CDS             complement(535..1566)
                     /locus_tag="ACHAYA_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8606671.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          584 a          319 c          328 g          670 t
ORIGIN      
        1 aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt ttgtctcatc
       61 atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg ttgttgttgg
      121 tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa cagctagaag
      181 gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt tcactccaag
      241 atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt aagttgtttc
      301 aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg tctcggaaaa
      361 gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg ttatggtctc
      421 catcattcac actccattat cttttgttaa gtaatataca aattcaagat aatcgtaaca
      481 taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt tgttttatat
      541 ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca tcttttccca
      601 gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat ttaccgcgtc
      661 agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata gttctggcga
      721 tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag gagaaatttg
      781 ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg ctgttaactt
      841 aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat tcagtctgtt
      901 ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac gtttaagttc
      961 tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg caaggttcct
     1021 cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt caatcgggat
     1081 aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag ctgctttggt
     1141 tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc ctttttgata
     1201 caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt aatagctatt
     1261 ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag tatgtcgata
     1321 gattttagct aagaaattct gataatcttg tttttctaaa tggataatca gctgatcatc
     1381 cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat cttttgcgta
     1441 ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact gttgaaagac
     1501 ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc tagttttgct
     1561 gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca tttcatcaaa
     1621 tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt actagctaaa
     1681 agatatgaag taagaagata catttttagt tattttatta gtgcagtcat tcttttgatc
     1741 ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat gaaatggatt
     1801 tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag aaaacatttt
     1861 ttcagaatcg attggctctc catttattca ataatggttt g
//
LOCUS       DAYYCW010000203         1872 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000203 DAYYCW010000000
VERSION     DAYYCW010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.210"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..1186)
                     /locus_tag="ACHAYA_002950"
     CDS             complement(146..1186)
                     /locus_tag="ACHAYA_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8606672.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          643 a          278 c          226 g          725 t
ORIGIN      
        1 ttgtgatttt tatcataata ttttaactta taaattctat taaaatagct attattttgt
       61 aatattcgta atataattag aataataaaa aatttttaat tttaatgtta ctccatgaag
      121 caacaatcac tttgtcagta aagtttcact ctaaccagtc tttcgtaata gtgcttgggg
      181 agcttgcata attcaaacgt ttttcacttg atactttagc agctattggt ttttcgataa
      241 accaattttt tacatagaca aaaatcaggt tctggatatt ttcgactttt tcggttttta
      301 atttttgata ggcttttaac agtgtcgtgt ataattcttg ttctgcattg acataataac
      361 tatcgagttc ttcaaaaact atcgaaatat cactttctat ttcagctttt ttctttgcat
      421 tatgaatagt ctggacggtt ttttgagctt cactgtaact tgaagaaaat gtttttatca
      481 aatttaatac tcgttcaggg acaaacgtag caatgctgtc gtttgtcata attgtctcaa
      541 aattgtctaa caacaattga tcttgtaggc ggtctttttc agtgtctata ttgtgtctat
      601 tagtgtctaa tatattatta ttaagttctg gtatgatttt cataccctct gatttatcaa
      661 cgttttggca cggtatgatt ttcataccct cttgagcccc aagggattca gcgtctgatt
      721 tttcgtcgag ggtgggtatg attttcatac cctctgattt atcaacgttt tccctattct
      781 ctctatcaaa tttttcgagt aagtagatat cattttctga gacttttaat tctgctagat
      841 atagtctatt aggattattt ttccctaact gtttgttaaa tcccatctgc ttttgtaata
      901 ataaaccaaa ggattccaac tcttttttga ttgctatcgc tttttgtttt tggcagttta
      961 atactctaca tagttcattt gtcgtataag taaaataaac attaccttct tcatcgattt
     1021 gccgtttgct gattgcgatc tctaatcttg cttttaaaag catataggca atcttagttg
     1081 aatctttcat ttttttgtat tcgtcagaaa caataaagac ttttggaaat tgataaaata
     1141 attcgttgta aactctatcc gccttaaaaa atttaaaatc agtcattgta gatcctctac
     1201 tttcacgttg ttttatttat gtaaaattac ctactctttt cgaaaatagc aaagataaat
     1261 aaaaaatgca aacgcattaa aactgttttt taattaaata ctacctaaaa tcagaaataa
     1321 aatgaattat gtactacctt taaagtcatt aagaactact ttaaataaat attatttata
     1381 attaaatact accagaaaaa cgtatgaata aaatttagtt ttaaaattaa agataattaa
     1441 gtactaccag tgtcaaaaca aaaaataaat taagaactac cttatgcata taaaacatac
     1501 taccattact agtaatatat actaccttat ttcagtagta tatattatat tattcttata
     1561 agacaaggct ttaggctaga aaacaataga ttatatacta ctaaaaaata caataagtac
     1621 taccaatgtc agatatatat aataattatg tactaccatt aaaattaact tatttaataa
     1681 gatactactg tgttaatctg aaaaaaatta ctttaaagta gttacatact acctgttaat
     1741 ttagatacta ccattaatac ttaaattaga tactacctat atttatatta agtactaccg
     1801 ttctaagaat aagataataa aaaaatacta ccaaattgca attaaatact acttaaaatg
     1861 taaaaaataa ga
//
LOCUS       DAYYCW010000204         1861 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000204 DAYYCW010000000
VERSION     DAYYCW010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.211"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..414)
                     /locus_tag="ACHAYA_002951"
     CDS             complement(<1..414)
                     /locus_tag="ACHAYA_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606673.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIP"
     gene            380..>1861
                     /locus_tag="ACHAYA_002952"
     CDS             380..>1861
                     /locus_tag="ACHAYA_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606674.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIP"
BASE COUNT          602 a          280 c          261 g          718 t
ORIGIN      
        1 aggaattatt gtaagaaaag gataaaaata gttcagtcca tctccataat tgttaaatgt
       61 tgtatagttg attggccctt caaaaataga agataaccct ttgattcgtg aaagatgaaa
      121 agataaatca cctgttgtat aaatttcttg agcatttctc ataaaattta ctgaatataa
      181 agtactaagt aagataagaa ctactaaaag aaatgctttt ttacttattt tcatagattt
      241 gttgttctcc ttttttaatt ctattttcag tatactagaa ttgtcttttt ttaataagtt
      301 ttgcgttaaa tttttcatag cactagtatg atatactaga aaagaatcaa tttttattac
      361 ttttaaatag gagaaaaaaa tgaaaaaaaa caaaaaccaa ataatagata tcatttttat
      421 gttatttcta agttttgctt acgtcatccc tctattcatg tctaagggaa tctatcatag
      481 tgtcaatcag gatacttact ttcatttatc tcgaatcata ggactagata atgtatggag
      541 ttctccagta aacttcaata acttcgatca tcatggaaca atgatgaata ttttttatcc
      601 atggcttact ctttatccag cttttttatt ttataaaatg atgggaaatc ttgttttaag
      661 ttataatatc tactattttt ttatcacttt tttaacaatg gtcgtttctt atttttctat
      721 gaaacaaata aaaaacaatc gttatatttc gctgttattt tcgattattt atacattttc
      781 cgcctatcgt gccattgaca tattccatcg tgcttcattg ggagaagctg tcgctttaac
      841 atttttgcct ttgatactaa tggggtgtta cgaaatttat attagagatt accaaaaatg
      901 gtactggttg tctattggca tgacgctagt agtgtatact catttacttt ctgtcgctat
      961 ggtttctgta tttattggag gaacgttgtt tctttctttt tatttttggg accaaaaaat
     1021 cgctagactc ttaagtcttt taaaagctac cgtcctcacc ttttttttaa gtgcgggatt
     1081 cttaattcct tttattcaac aatctagagc acaagaattg aaagtcccac tgggaaaaga
     1141 attaagtggt atggctccta gtgatatgct gacacatatt ttaaataata actataacaa
     1201 ctatactatt ggattattct tatttctcgg attgattggt gcttttattt ttataaaaaa
     1261 attaactgct gatgatttat ttattttctt tcttggtgta tttgttcttt tttgttcaac
     1321 caatttattt ccttggcaac tttttaacca tacaccagtt aagagtctac aatttgtctg
     1381 gcggttaaat ggtttttctt ctttatttat agcctatacg atgagtatag tgatctatta
     1441 tattttttct actaaaaata attcatggaa aataatggtc tttacgtttc ttgcactaat
     1501 attgcatctc tcaggagtaa gcaattctat ccatgctaac aaagattcat taacacttat
     1561 agcacctgaa gacgctgttg ctatagctga aaattacaat catactgatt atgcaaacaa
     1621 agagtcaatc tatcatcctg atatgattaa taatgatcaa tatttactag gtaaccagga
     1681 aataaatcca acaactcatt ttatgagtaa caaacttaca attgaattag ataattcgaa
     1741 taataaaaat actgtcttga cgacacctat ttatcgttat aaagggcaag tggcttctat
     1801 taatggcaaa ctagtacaaa caaaattatc taagtttggc acaactgaat taactattcc
     1861 t
//
LOCUS       DAYYCW010000205         1861 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000205 DAYYCW010000000
VERSION     DAYYCW010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.212"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..846)
                     /locus_tag="ACHAYA_002953"
     CDS             complement(<1..846)
                     /locus_tag="ACHAYA_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8606675.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYER"
     gene            complement(910..1269)
                     /locus_tag="ACHAYA_002954"
                     /pseudo
     CDS             complement(910..1269)
                     /locus_tag="ACHAYA_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(1447..1767)
                     /locus_tag="ACHAYA_002955"
                     /pseudo
     CDS             complement(1447..1767)
                     /locus_tag="ACHAYA_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          561 a          374 c          265 g          661 t
ORIGIN      
        1 tctttcataa agagaacgac tgtctaatgt tggaagtgtt agctttacag gtattcttct
       61 caacaaagtt gataatagtt tatcctcagg tggttctgat gttgcaaata taatccagca
      121 acttgatgtg taccagtttt cattatcacc catccgatga aatttcctcg tatccataaa
      181 ctgaaaaagt ttctcctgac attcattgct caatgcatga atttcatcta aaaataagac
      241 tccattatta gctaattctg ctaaaccctt tcttccatct tcggctcctg tatatgcacc
      301 ttttacatgc ccaaaaaaat tggacaagaa aagttctgga ttattggcat attcactaca
      361 attaactgaa atgaaagatg ccttctcttc tatataatta ttggccaaca aatccttata
      421 cacataattt gctaaatagc tttttccagt tccagtctcg ccatgaagta aaatgggtaa
      481 gccatttggc ggatagtaga ttgctgcttt aatttgagca attacatttc gcagacttcc
      541 ttgacttccg ataacccttt ctattccaac cctaaaagag ctatcatacc atttttccgc
      601 ttcctctgaa gagctaaaaa tttgttgatt tggaactttc tccggtaaag aaaagaagat
      661 tactggacga gtattgattt ttccaaattc tccatctttt acgaattcat tcaaatattg
      721 agagacaata ttacgactaa catttaattt ttcagatatt ttctgtgctg tattgttccc
      781 ccatttattt ttaatctcag agttctctat gacataattg acaatgcgtt cttttaattc
      841 ttccatcaca gcacctcctc gacactattc gaattttgcc gacagtattt ttccataaat
      901 atgacttgct aaaaaggctt tgtttagatc attcaattgg aacgttgctt gttttgcttg
      961 atccgctaag acgttgaaaa gatactctgg cgaaatcgca taccccaaaa tatcaacgat
     1021 tgttgcaatc agatcttctt ttgattcttc atctgaaagt aattcacgat acaacatcgt
     1081 ttgtttgctt actgtgtcat attcctctaa ggattcatct gctaaaagaa ccacttgttc
     1141 caacaaacgg tcggataaat atttatagaa aatcagcccc aataaataat ttttatattc
     1201 tgacgcatcc attttactac ggagattatc tgccgcacta aataacttct gatttaattc
     1261 tgcacccaaa aagatacact cctatctctt atttaaagtt gctatatacc ctcttatttt
     1321 agcatactca gttttagaaa aagcaaaaaa ataaagaaac ttattcttga taacaagcta
     1381 cgtgacgtat atccctaaca caaaaatagc catatccttt ggtttggata cggctatttg
     1441 atattactca ttcaattatc aaatcacagt aggtgtgaat ctcatcggga tcggcaatat
     1501 ctacttctga ttaggatcaa aggtgatttt ctcaataaag gtgtgataca aagctttaac
     1561 ttgcttcttc tcactctgga taagaaagca atccaaacta gtcaatatct gttggacatc
     1621 tttgacttga atcggttccc cttgttgttg aaagatccct aagatttcat tctctcgaat
     1681 ttgtaactgt cttcgttttt cagtcaattc atttaaacga tcttttaaca tcggaaacaa
     1741 ttcgggacta tcttccaaag cagcttcctg tttttgctct aatagttaga ttttgcgtac
     1801 cattttcttc ttttattttc cttcgattgc tgattttaat atagtctaaa ggaaatttat
     1861 a
//
LOCUS       DAYYCW010000206         1859 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000206 DAYYCW010000000
VERSION     DAYYCW010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.213"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="ACHAYA_002956"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="ACHAYA_002956"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8606676.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DAYYCW010000207         1832 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000207 DAYYCW010000000
VERSION     DAYYCW010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.214"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            53..862
                     /locus_tag="ACHAYA_002957"
     CDS             53..862
                     /locus_tag="ACHAYA_002957"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8606677.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            902..1399
                     /locus_tag="ACHAYA_002958"
     CDS             902..1399
                     /locus_tag="ACHAYA_002958"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM8606678.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT          638 a          327 c          351 g          516 t
ORIGIN      
        1 aatcttaagg taacaggaaa atagatatgt aaaaagaaag taggaagata aaatgaaact
       61 agcagcgatt gatttagacg gtaccttgct tgatcgaaat ggaaagatac cagaagaaaa
      121 tatccaagca cttcggaaat ttgatgaaaa tggcggaatc gtaaccgtcg ctaccggaag
      181 gaacagcatt tctgctaaag atattttttc cgaattagga atcggtggct atttgatttc
      241 ttccaatggt tcactgattg ctgaaatgaa ggatggaaaa atggcgaaag ttctcagacg
      301 ttcaaaaata gatagcgata cactgagaaa agccttcttc tatgctcaag aagcgaaaat
      361 atccatcatt gctagccgag aaacccaaga tgatcagctg acattcgatg aatcaaatct
      421 agtcaaagat gacccttatt atgaacattt caatttacag aaccattctt ttgatcaaat
      481 tttaaatatg ttggaagatc cttctcttag ctatcttaaa ttagcattaa cagataagag
      541 tgaagaaaaa ctacagaagc tacaaagaga tctgcaagca gatggtatcc aatcagtatt
      601 ctctgatccg catttcttag aaatcacgcc tacaggtatc actaaagctc attcacttgt
      661 ttttctggcg gattaccttg gaatcgaatc gagtgacatc atggcttttg gcgatcaaga
      721 aaacgacatt gctatgttga attacagcgg gttgagtatt gcgatgggga atgcacagcg
      781 acaagtaaaa gaaattgcaa accaagtaac actgacgaat gaagaagctg gtgttgctta
      841 tattttaaaa aacttctatt aaaaagaata aaatgacgat atgaatgtag gaggaaagaa
      901 aatgttagtt attattgata tgcaaaatca tattcttgat ccaaacagcg aattttatat
      961 tccagagtca gatcaattgg cagaacgtat tgctcaaaga ttagctaaag cacgccaaaa
     1021 taatgaatat attctcttca cacgggatat accagttgaa ctgaaggaca aagaagaaga
     1081 acgagaagat ttgcaattga tccctcttct ttctccacaa gaaaatgaac gagtgattaa
     1141 aaagtattac tttacgattc cacctgaaac tttagctgaa atccaggaaa ctttatttga
     1201 aagcaaagaa gaacaaaaag agatcgaagt agtcgggatt gaaacaaact tgtgtgtact
     1261 ctctaatctg ataggcctgc agagtgcttt tcctgaagca gacttttttg ttgatccaat
     1321 gttagtctcc agtagaaaac acggagaatc cgctttagag ctgttgaaag atttcaacgt
     1381 ctctatcgtc gaatcataaa aatatctatc ttcatttacg atatttaaca cacaataaaa
     1441 aaagcacgca gaactgtttt agtcctacgt gctttttcta tcaaagtaga ttggcttttt
     1501 tatgacacag agtgaaacgg caggctcacg acctctttct taaatggtgt tcaatcagct
     1561 gaccctcggt attagcttaa caaacggcaa aatatgagga actatttttc cgtttgttaa
     1621 gctaatactc aggtcaaaac gctgatttca caacctcttc cttaaacggt attcaaaagc
     1681 ttgcatctgc agtaataaga acagccaagt aaaaacataa gaagctgttt ttacttggct
     1741 gttcttatta cttgatgcag ggcagctttt tcacaacctc ttatgtacct tgtaggactc
     1801 gaacctacga ccggacggtt atgagccgtc tg
//
LOCUS       DAYYCW010000208         1760 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000208 DAYYCW010000000
VERSION     DAYYCW010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.215"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACHAYA_002959"
     CDS             278..820
                     /locus_tag="ACHAYA_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606679.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..1425
                     /locus_tag="ACHAYA_002960"
     CDS             976..1425
                     /locus_tag="ACHAYA_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM8606680.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1455..>1760)
                     /locus_tag="ACHAYA_002961"
     CDS             complement(1455..>1760)
                     /locus_tag="ACHAYA_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8606681.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          655 a          256 c          334 g          515 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccagatggaa tggcaacact ttttttgaca aaaattataa ggtgttgtga atgctgcggt
     1381 gttatggcta cgattgccct tgacaatgag catcctcgga tctgattttc tccggcagaa
     1441 gcacaatcat tcaattacgc tgcctctcca gaggtatcag atccgcgctc attatcaagg
     1501 attcgcttcg ccaatctctg ttgtcgaatt tggatcggtg tttcatacgc caacgtgcca
     1561 tgtaagcgta gatagttcca ccaatgcaca taatcaaaca attctagacg tagctgtgcc
     1621 agtgtctcaa attggtattg atgcacgaat tctactttga cagatttata ggttgattcc
     1681 acaacggcat tatcatacgg acaacccttc ctactcaatg aacgagtaat tccaaaacca
     1741 ttcagaatct catgaatggt
//
LOCUS       DAYYCW010000209         1672 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000209 DAYYCW010000000
VERSION     DAYYCW010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.217"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..535
                     /locus_tag="ACHAYA_002962"
     CDS             <1..535
                     /locus_tag="ACHAYA_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606682.1"
                     /translation="IDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETD
                     IFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPI
                     STISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKIL
                     LPENWAELRKLVEYGII"
     gene            522..>1672
                     /locus_tag="ACHAYA_002963"
     CDS             522..>1672
                     /locus_tag="ACHAYA_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8606683.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLG"
BASE COUNT          547 a          215 c          271 g          639 t
ORIGIN      
        1 aatagatgaa aacagtaaat tgatgaaaaa catacaatca atctttggga tagaagaagt
       61 ttttgtcgga gataaatgta tcttttttga tcaaccttta ttatctgatg gttatggcat
      121 agatgaaaca gatattttcg ctaaagtaaa tagtatattt tctggaaaaa aagaaacttt
      181 aattaagctt catcctagag caaggacaaa tcgttatggt gaaaaaaaca tattgaattc
      241 cagtttaccg tttgaagtct tgctacttga aaatgacttc tcttcaacac ttttgattag
      301 tcctatatct acaatttctt tttcaccatc tatgatgttt gaaaagacga tcaactctat
      361 tatgttggct aaattaattt taaatgaata tacagcatta tctgaagata aaaaagaaac
      421 attgacaaat attgttcttt tctgtgaaca ttataaccgt ctatcagaag aaaaaatttt
      481 actcccagaa aactgggcag aattaagaaa gttggtagag tatgggatta tttaacacac
      541 tttccaagtc aaaattagtt aagtccagtt tctggtatac tatagggaat ttttttatta
      601 agggaatttc attcataact attcctattt ttgtaaatat tactactcaa gcagaatatg
      661 gtttgattaa taatttcact tcatttgtat caattttttc gattcttgtt gggctaagcc
      721 taaattcggc aattaataat gcaagttttg actataaaga tgacataaat gaatttatgt
      781 catcggtact ttctctagct acactttcgt taattgtgtt cttattaata gggaacatat
      841 attttttgtt ttcaaatgct ttttttgata tgaaccaact tgtatttaat ttgatgttat
      901 tacaaagttt tgctagtttt gtaattagtt atatccagtc ttattttacg attaacttta
      961 accattttaa atatttgatt ttatctttta tatctacttt tttgaatatt gcaatctctg
     1021 tagctttgat gcagacatta tattttgatg ataaatatgt tggcagagta gtcggcggaa
     1081 cactgtcatt tgtgttgata ggtttagtaa tttatttaag aataatgata aagggtgctc
     1141 atttttttga taaaaaacag tggagatacg ctttaaaaat ctctttgccg ctaatcccac
     1201 attccttagc aaatatagtt ttagcacaat tcgatagaat tatgattaat tcttatttag
     1261 gtcctgctgc ggggggaata tattcctaca ttagtaacat aggtattatt ctaagcgtaa
     1321 tatggatgtc tactaataac gcctgggttc catggttcta tggtgagatg agtaaaaata
     1381 atgaaaaaaa agtgaagaaa gttagtaact attatttagt agtctttact ttgattacga
     1441 tgattgtaat ggtagtttcg attgatttag caaaatttat ggctccgaaa gactattatt
     1501 ctgggttatc tttagtggtt ccgataacct taggctattt ctttcagttt ttatatagtt
     1561 taccagttaa tgctgaattt tatgagaaaa aaacatcgtt tattgcgatt ggtacagtag
     1621 caagtgctat agttaatata ctattaaaca tgattttcat acctaggtta gg
//
LOCUS       DAYYCW010000210         1654 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000210 DAYYCW010000000
VERSION     DAYYCW010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.218"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..597
                     /locus_tag="ACHAYA_002964"
     CDS             175..597
                     /locus_tag="ACHAYA_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606684.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            607..810
                     /locus_tag="ACHAYA_002965"
     CDS             607..810
                     /locus_tag="ACHAYA_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606685.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1021..1605
                     /locus_tag="ACHAYA_002966"
     CDS             1021..1605
                     /locus_tag="ACHAYA_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8606686.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          589 a          231 c          297 g          537 t
ORIGIN      
        1 gtcacttttc tttttaaatg gcaatatttt ttgtaaataa atgtcaatca agaaaattta
       61 aacgattatg acattaaaaa tatattttat gaattttcta gtatataact tgatttatct
      121 gttaagattt attaagaaaa cgttttctta tttttgattt cgaggtgata gacaatgaaa
      181 aataagttta ttttatcttt aggtatttta actattggaa ttagtgtaac tggaactaca
      241 attcctacat ttgctgatag tacaaatgaa ccaactactg tagtagataa taaccaagaa
      301 ttaactcctt tagataggaa cggaatagcc attggtaacc caactaacga cttaactgtt
      361 actgaaaatt atttaatagt ttctccatta cttgctcgag cagcttctaa aacgatacga
      421 gtgacaggaa cgatgatttt tgataaagtt ggttggcctt ctagacctcc tcaatcacgg
      481 aattggaaag aaaaaagaaa tggttattgg tactatggaa aattgtattt acaaagttat
      541 aaatcagctg gaaacaattg gattgcgact tattcaggaa ccttaactag aaagtaggga
      601 ttttttatgc cagaatttgt gaacacacca aatttttatc aagatagcga tattctaagt
      661 aaaattaatt taccgaaaga agaatcagct ttacaagttc agcgaacttt acaggagaca
      721 caaaaaattc aaaaatcaat ttctaatcta gaagattctg aagaaaaaag aaaaaaaaga
      781 gaccaaaaaa catatcaaaa agaaaaataa tgattttaaa aaaagagtgt ctagtcgaca
      841 ttcttttttt ctaattgaaa gtgttcataa aatcacccat ttctggacac tttctttttt
      901 tgctttattt ttcaaaaaaa caattcattt catgttcata aacccataac aatatctatg
      961 ttcagttaaa ctatatggat aaagttatgt tacaataaaa ttaatgaagg agtgattttt
     1021 ttgaaaatag gatacgcccg ggtatctact ggtttacaaa atttggattt acaagaagac
     1081 agcctaaata aatttggttg tgaaaaaatt tttactgatc atatgagtgg ggcaaaaagt
     1141 aatcgaccag gattagaaat ggccattgat tttgttcgct ctggcgatac acttgtcgtt
     1201 tggcgcttag atcgtttagg tagaaatatg gaagatttga tttcaatcgt caatcgactg
     1261 aatgagcgtg gtgtgagttt tcatagtatt caagaaaata ttacgatgga caaatcaagt
     1321 tctacaggac agctcatgtt tcatttgttt gcggcatttg cagaatttga gcgtaatttg
     1381 atcctggaac gatccgcagc aggacgagca gctgctcgtg caagaggacg gtttggtggt
     1441 cgtcctgaaa aattgtctga acaagatttg gaattactga agactttagt ggatagcggt
     1501 acacctatta agactattgc ggaacggtgg aacgtctcaa gaaccaccat ttatcgttat
     1561 ttggcaaaaa tggaggagaa aaatggaaag acaaattctc attagttttt gtcatttaat
     1621 gctgaaaatt tttgccattt atctagaaaa gtga
//
LOCUS       DAYYCW010000211         1645 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000211 DAYYCW010000000
VERSION     DAYYCW010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.219"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..403
                     /locus_tag="ACHAYA_002967"
     CDS             5..403
                     /locus_tag="ACHAYA_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8606687.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1081
                     /locus_tag="ACHAYA_002968"
                     /pseudo
     CDS             387..1081
                     /locus_tag="ACHAYA_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
BASE COUNT          623 a          238 c          324 g          460 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcagcgata acgttaggta aagagctact
      961 agatcaagta ttagagatgc cctcgattga taaagcagtg aggggcttcc aagaagattg
     1021 ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa aaggtcgctg attacttctg
     1081 aatcaatttc tccataaaat aaacgatagc cttctcgaaa actcgaggag gctatctgac
     1141 gattcaatat tcattaattt aaagtttggg tgactattac taactattac ttatttctca
     1201 tttaaacgat gtattcccta acctctaata catccttgta actctgaagt tacgccagtt
     1261 ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata aaaatatctt tcgtgtttct
     1321 ctagtataac agaataagaa gaacaagtcc agaagggata tttttagtct tttttaaaat
     1381 aacttttcag tcgagtttga gtttatgata gttgtttttg aagcgatacg gcaagtgaaa
     1441 aaatagggaa gcatgaaaga atctacttaa tggcgcattg attcatgtga tagaaataaa
     1501 aaactttttt aaattatatc ctcatgattg taacaaatcc agtgatttgt tttggtacaa
     1561 ataaaaaaag gccccgatga agttgtggca aaagtggttt gcctcaaaaa taagaaggaa
     1621 tttccaaaaa tcacttctca aattt
//
LOCUS       DAYYCW010000212         1639 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000212 DAYYCW010000000
VERSION     DAYYCW010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.220"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1604
                     /locus_tag="ACHAYA_002969"
     CDS             <1..1604
                     /locus_tag="ACHAYA_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFM8606688.1"
                     /translation="ETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHK
                     MFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPI
                     REGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYN
                     GDINNRLARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFS
                     RTNRTYPEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQA
                     YGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQ
                     IQLLEEQIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTY
                     AANSSDAREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQAS
                     DNLIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFK
                     YPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          601 a          269 c          334 g          435 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgtacg attcaaaga
//
LOCUS       DAYYCW010000213         1630 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000213 DAYYCW010000000
VERSION     DAYYCW010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.221"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..924
                     /locus_tag="ACHAYA_002970"
     CDS             13..924
                     /locus_tag="ACHAYA_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8606689.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT          618 a          183 c          327 g          502 t
ORIGIN      
        1 ggtgcttttt tcatggttga taattcaagt ggaagggttc ttaaatctct aagaaaagag
       61 aaaaaactat ctcaaaaaaa gttagctgat ttagcaggta tttcacaaag tactttagtt
      121 aaatatgaaa aagggtcaag aaaaatccct aaagacgtag ataatactct aagcaagatt
      181 ctgaatatag aaactctaat aaaagatgag gaagataaga ttgagattct catcggtaaa
      241 ctaattgcat acagagatat gaataagtta cttaacaaag agttagctga taatatagga
      301 attagtgaag tattactcag ttatgtgtta aatagaaaga gaaatccgtc aaaagaaatg
      361 caaaaaaaaa tcgatatttt tctattaagt aatgaaaaag aaattttgaa ggagattaat
      421 cgtgatagtg agatatttag tttatcaaaa gacgataaaa ttgttatggg aaaaagaata
      481 cgagaagtcc gaaagaaccg ggaagagacg cttgaaaaat ttggtaaaaa ctttaccatc
      541 tatacaggca aaaatgttat aagtagatgg gaaaaaggta ttaatattcc agatattgaa
      601 aaactaatga atattgccta tcttggtaag gtcactgttc cttaccttat gtacggagaa
      661 gattataaga atatacttcc aaaagatgaa agagttagcg attttaaaaa gataaattct
      721 tttagtatgg gattaaggat gaggaaaatt aggaaagatt attatctaga aagggaggag
      781 tttggaaagc ttttttctcc ttcaatttca aaatggtcca tagataggta tgagaatggt
      841 agagatatac ccaatactaa tcgaattatc caatatgctt atataggaaa tctttcttta
      901 gaatttttga tttacgggat ataaaaatag tttacgtcga tacttattaa gttatcgacg
      961 ttttttatta tagaaaaaat ttataaaagg aggtttcttg atatgataag aaacaaaaag
     1021 ggtttgttga aagttgcttt actggcagct tttttctaag taactttttg acaagctcaa
     1081 tggcaacaca agcggaagaa gtaatacaag aactaactgt tatgaataac agctagataa
     1141 aatgggctat gtatgttatc caacaattta gtaatcacta gaagcctagc gaaagttcgc
     1201 ttgataagtt atagatgagt attgatttgt agtgtttcca aatcaaaagg gtacagagcc
     1261 acatgaaata tgttgtcctc tgcagcgtca ggatgggaat ccataccatg gagattcgga
     1321 gatctgtgag ggaatactta cgtggttaat atgtcagttg tgttggacgt agtaatggtc
     1381 atagtgggta gattgactga attcttatat gagaaaatga aaccaacaag aagatagggt
     1441 tatttataca gaattgggga tcttttctgg aaaagtaatc ggataatgcg agattcgtac
     1501 ggtctttttt aaatttctct aaaaaaaacg gcatcgaatg ggttgggtga ttgataagca
     1561 atgaatcata gactgatata gaataaaaaa tgattctatt tatcttttta catctatcgt
     1621 tttttcaagt
//
LOCUS       DAYYCW010000214         1627 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000214 DAYYCW010000000
VERSION     DAYYCW010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.222"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..613)
                     /locus_tag="ACHAYA_002971"
     CDS             complement(<1..613)
                     /locus_tag="ACHAYA_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8606690.1"
                     /translation="MDNHTRKLLVLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
     gene            901..1554
                     /locus_tag="ACHAYA_002972"
     CDS             901..1554
                     /locus_tag="ACHAYA_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8606691.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          585 a          225 c          300 g          517 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tggctggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttctatga gaatcgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ctgcccacaa tcatagcata
      361 gaaaacgggt acgttttaat tctaaaatag tcaacttttc tttgactttc aatatctgag
      421 tacgtgtcgt ataggaaccg ttgcggataa tacgaatcga tccacagtgt tcacaatggg
      481 aaggtgagtc atccaatctc cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatacgagt aattttctag
      601 tatgattatc catagagcga ctcctttggt agggtgatgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccaa caacacaaaa tattatagaa ccctttattc taaaggattt caaggtgtct
      781 gtttgcattt attagaaaaa agaaaaagta ttggcaacag aaggtgtcac caacactaga
      841 tattatagag ccataatatg ctgtataata tctagtgtat aggcttaagt ggaggtatat
      901 atgaaaatat taactgttga agatgataat atgataagag agggaataag tgaatatctt
      961 tcagaatttg gatatactgt tattcaagct aaagatggaa gagaagcctt gtcaaaattt
     1021 aatagcgata taaatttggt tatcttagac attcagatac cttttataaa tggtttagaa
     1081 gtgttgaaag aaattagaaa gaaaagcaat ttaccaattc taatcttgac tgcatttagt
     1141 gatgaagaat ataaaattga tgcatttact aatttagtag acggatatgt agaaaagcca
     1201 ttttcattgc cggtcttaaa agctagaata gattctttaa ttaagaagaa ttatggacat
     1261 ttagagaagt ttgaatataa ggatctatcg gttaatttta atagctatac agctaaaata
     1321 aatggtgaag aaatagatgt aaatgcaaaa gaactagaag tattaaaatg tttattggat
     1381 aataatggac aagtgttaac aagaatgcaa attattgatt atgtatggaa agatagcgaa
     1441 gaaactcctt atgatcgagt aatagatgta tatataaaag aacttagaaa aaaattacaa
     1501 ttagactgta taactactat aagaaatgta ggatataaat tggagagaaa atgaaaaaat
     1561 taaagatatt tccaaaaatg ttcatacaaa tattttctgt tcttggaata ataattatat
     1621 tagttca
//
LOCUS       DAYYCW010000215         1612 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000215 DAYYCW010000000
VERSION     DAYYCW010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.223"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1612
                     /locus_tag="ACHAYA_002973"
     CDS             <1..>1612
                     /locus_tag="ACHAYA_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8606692.1"
                     /translation="KDIDTFLNTVTIYAQSRTNRLGRRVVTLVEVDSKTGQEAISIIE
                     TKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLLQYWGGEIKREPFKLSLLK
                     RRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQSEVGTTNRIYGSPVDSAYI
                     NNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGCDKPKVSITVEFDKLTDSE
                     EAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSLSEKTLKLEAGDIQVAFYQ
                     QQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYVVLWPKEKPSNIFIMDNPD
                     LEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANFIQTGLIKADIFQNSFNKT
                     GDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTIGTTDSVGNPFPGASTPTP
                     IEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGDLDVQGKLTVKGKEIIPGQ
                     GGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT          592 a          203 c          326 g          491 t
ORIGIN      
        1 ataaagatat agatactttt ttaaatacag taactattta tgctcagagt cgtacaaatc
       61 gacttggaag acgggtagtc actcttgtgg aagtggactc taaaactggt caagaagcga
      121 tatcaattat tgaaactaaa atggataaaa aatctgacat acgactttat tctgatatta
      181 cggctgtttc tagcacaatc tttgaagcga gaaatgtttt aaattgtatt gctggtgaac
      241 aaggatcatt gcttcaatat tggggcggag aaattaaacg tgaaccattt aagctttctt
      301 tgttaaagcg aagaggtcgc aataatgttg gaacgattcg gtatggaaaa gatatgtctg
      361 gtttgaaggt caaattagat tggacaggta taaaaacaag aattattcca tatgctgatc
      421 ctcaaagtga agtaggtacg actaatcgaa tttatggttc gccagtagat agtgcctata
      481 ttaataacta tcctgatgtc tatacagaac atgttcagtt tacagaagaa caaggagtaa
      541 aagacattaa gagcttaaat aaaatagcta aaaattactt taaaacgatc aatcctggct
      601 gtgataagcc taaagtttct attacggttg aatttgataa gttgactgat agtgaagaag
      661 cgaaagaatt tgcgaagatt agaaattatg gtctgtttga tacatttaaa atatatcata
      721 aaaaatataa tatttatctt gaatcaaagg ttagtggact acaatacgat tctttatctg
      781 aaaaaacttt gaaattagaa gcaggagaca ttcaagttgc tttttatcag caacaagctg
      841 taactattca ggataaatta aaagattatg caacgaataa ttatatgagt gattttaatg
      901 attatgtttc ttcaatgatt acaggtcaag gcagtgcagg aggatatgta gttttatggc
      961 caaaagaaaa accttccaac atctttatta tggataatcc agatttagaa aaggcaagag
     1021 aagtactaag aatgaataaa aatgggattg ctttttctaa gaaaggatgg cgggggcctt
     1081 ttaattcagc ttggacatta gatagtatat ttaatgccaa ttttatacaa actggattaa
     1141 tcaaagcaga tatctttcaa aattctttta acaagactgg agatgtattg aaattagtta
     1201 atggactact ccaaatttgg aacaacaaaa agaaaataat ggagttaact aaaaaaggaa
     1261 tggaattttg gaattctaag ggttcaattg gaacgattgg aacaactgat tctgttggta
     1321 atccttttcc tggggcttct actccaaccc ctattgaaga taattcttta gttattcgta
     1381 caaatggaga cggcaaatat attttgattt ctcctaaagt aggaaaagga ttagttttat
     1441 taggaaatgg taaagcaatt tattttggag acttagatgt acaaggaaaa cttacagtta
     1501 aaggaaaaga aataattcct ggacaaggtg gtggtccatc gggaggtgga gaatctactg
     1561 gtgggtatcc tagtgaatta aaaacagatg cagaaaagag agcttggaga at
//
LOCUS       DAYYCW010000216         1587 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000216 DAYYCW010000000
VERSION     DAYYCW010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.224"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..682
                     /locus_tag="ACHAYA_002974"
     CDS             <1..682
                     /locus_tag="ACHAYA_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8606693.1"
                     /translation="NIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVNSAENAWT
                     NLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNI
                     KNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVN
                     GISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGA
                     VGGTLYAT"
     gene            672..1412
                     /locus_tag="ACHAYA_002975"
     CDS             672..1412
                     /locus_tag="ACHAYA_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8606694.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
BASE COUNT          611 a          184 c          293 g          499 t
ORIGIN      
        1 aaatattaaa gatacggcat acaatacgtg gatttctata aaaaatacca tgattaatac
       61 atggaataac attaaggact ctttctggaa tattgttact ggaattgtta attctgctga
      121 aaatgcatgg acaaatctaa aaaatggtgt ttcaaatgct attaatcggg taaaagaaat
      181 ctttaattcg ttaagggaag tcaatttatt tgaaattggc aagaacatca ttgatggact
      241 tataaatggt gtaaaagaaa agtggaatgc tttgaaagat actatcaaag gtattgcagg
      301 taatatcaaa aattggatta aaggagcttt agatattcat tctccatcaa gatggatgcg
      361 tgatatggtt ggtaaaaata ttgttcaagg tattatcgtt ggtattgata aagagcaaag
      421 taagttagat caaaccatga caaatttagt aaaaacaccg tctgttcaac ctgtagtaaa
      481 tggaataagt cacctaccaa ttacacaaac aaagcaaaat atatctgcta gtgaaacaaa
      541 agaaattcat ttacatttaa atgtttatgg agatttacct gattcaatgg ttcgaaagat
      601 tactacaaaa attaaaacag aattgacaag acaaatgaaa cgagatgctg gtgcagtagg
      661 aggaacatta tatgcaactt aagagaggac aattttttat taatcaacat tattcttctg
      721 aatttaatgt gtatattcaa aacaggcctg cttctgtttc agctagtcga gtaattgaat
      781 tgagagagcg agaaggaaat gattctatca ttattgataa aggctattac aaaaatgtga
      841 ctagaaagat tgagtgttac tataaagcac cttcaattga tatggtgcag gagtgggaag
      901 atcgaatcac tgaatggtta gatatgtcgt cttatagtga ttttattctt tactatgatg
      961 aacaatatat ttatcaagct gtagtaatag aaggacctga atttaaagga acaagaaaaa
     1021 caggaaatat tgtccctttt gaatttacag tgagtattcg accatttaag gaaaattaca
     1081 atggtagatt tactattcga caaacagaaa cttttgaaat ttataaccca gagaagtatt
     1141 cttcaaaacc gcttattaaa ttgagtggtt ctggagatgc ttctttttat attaataagg
     1201 ataaatatga tttgaaatcg ttggacagag aattatttat agattctaaa ttagaagagg
     1261 cttatcgaaa attagatggt aatttagaac atcaggatca ggtcacctta tttttagatt
     1321 ttccattttt atatcctgga aaaaatgaaa ttaaatggac gaaaaatatt cattcatttg
     1381 aaataatgcc taggtggtgg agaaaagtat gaaaccaaga atatatagtc ctactgaaac
     1441 agatttctca acgaatggct taggaatttt aaaggattgt acaagatgtg aaatatatga
     1501 agtagctaat ggaaaatatg aattagagtt ggattatcct ttaggaacta gatttgatga
     1561 atattttgaa aatgactatc aaataaa
//
LOCUS       DAYYCW010000217         1581 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000217 DAYYCW010000000
VERSION     DAYYCW010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.225"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..231
                     /locus_tag="ACHAYA_002976"
     CDS             <1..231
                     /locus_tag="ACHAYA_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8606695.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
     gene            390..>1581
                     /locus_tag="ACHAYA_002977"
     CDS             390..>1581
                     /locus_tag="ACHAYA_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8606696.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLN"
BASE COUNT          508 a          305 c          294 g          474 t
ORIGIN      
        1 ttaaaaaatc ttcctgaaac acttgatgca caacttcgca cgaaacttca aaacctttta
       61 acgtatgaag aaggtattta caatgctatg atttacccgt attctaatgg caaaatagag
      121 gccaagattc cccatatcaa gacactgaaa cgattatctt atggatttaa atcatttgag
      181 aacatgaaaa tcaggatttt tttgatcaat caattgattc aagtgaaata acaaaaaaat
      241 ctgagacaga aggtcagtcc ttctatcggc tctatacttt ttgatgttgg tgaacttcac
      301 caacatcttt tttgtataaa aaaatataga aaagacaccc tgaaatcctt tagaattaat
      361 ttggctacca attcatagga ggattcaaga tgtctcatac tcagcttatc aaagataccc
      421 taaatattct agacctaaat atctatttcg aggaaaattg tttaactaaa gaaaaataca
      481 aagggcaaat ttgcttgatt tacaaaggaa ctcttctgta caagccagaa gagtgttccc
      541 attgtctttg tgtcgtcccc tctaggatca ttcgttgggg gacgacaacc gttcggttgt
      601 tactgaatga tgtgtctgag tatcgcactt atctggagct gaagaaacaa cgatttaaat
      661 gtaagtcttg ccaaagaaca ttcgtagcgg atacctctgt tgctgaaaaa cactgtttca
      721 ttagtcaaaa agttcgttgg tctgtcattg ctcgcttaaa agagaatact tccatgacgg
      781 aaatcgctcg tcagaaaaat atttccactt cttcggtcta tcgtgtcatg aagcgattct
      841 accgcccatt gaatcctttc aaacagactt tgccaaaggt cttatgcttt gatgaattca
      901 aatcggttcg aagtgttgcc agtgcaatga gttttatcat gatggacgga caaagccatg
      961 cattattgga tattgtcgaa aatcgacgct tgccttattt agaacgctat ttttctcggt
     1021 tttcattagc gactcgtgaa gccgttcaat tgattgttat tgatatgtat gcgccttatg
     1081 tttcgttggt gaaaaagctt ttcccgaatg ctcagttgat catcgatcgt ttccacatcg
     1141 tccaacatat tggcagaact ttcttgaatc atcgagtgaa agaaaccacg gtacgactta
     1201 aaggaaattc ccactctcaa cgagggttag ggaaaaaatt gaagcgctat tggaaactcc
     1261 tacaaaaaga agaagcaaag ttaaactatg aaaaacgggt ttggcgtgcc agttttaaag
     1321 attatttgac tgaaagtgag attgccgatc gactgttagc tgcttgtccc aacttacgcc
     1381 aaggctatca gttataccaa gacattcttt atgcggtaaa gaaaagagat cagtcactat
     1441 ttgaggattg tttaactaga gacattagcg gtctgccaga aacctataca accactttac
     1501 gaacgtttaa aaagtttctg ccacagatcc aaaatgcgct tcactacagc tattcaaacg
     1561 gaccattgga atgcttaaac a
//
LOCUS       DAYYCW010000218         1548 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000218 DAYYCW010000000
VERSION     DAYYCW010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.226"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..445
                     /locus_tag="ACHAYA_002978"
     CDS             191..445
                     /locus_tag="ACHAYA_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8606697.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ACHAYA_002979"
     CDS             446..763
                     /locus_tag="ACHAYA_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8606698.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..>1548
                     /locus_tag="ACHAYA_002980"
     CDS             1322..>1548
                     /locus_tag="ACHAYA_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606699.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKT"
BASE COUNT          549 a          222 c          295 g          482 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaac
//
LOCUS       DAYYCW010000219         1518 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000219 DAYYCW010000000
VERSION     DAYYCW010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.227"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..1145
                     /locus_tag="ACHAYA_002981"
     CDS             207..1145
                     /locus_tag="ACHAYA_002981"
                     /inference="COORDINATES: protein motif:HMM:NF016640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase domain-containing protein"
                     /protein_id="HFM8606700.1"
                     /translation="MGRQELYSLAINELEKRNLEIHELNQKNNSILGMKIRNKINQLN
                     QMKKRDNLLPAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSCIT
                     GNYDSIKEPDFSHPLVDYYLYTDNVITSEHWNIREIPKYISELKNNILINRYIKFHPN
                     ELFTDYDFTLYVDGNVTITSDITGLLNSSSKLGLSMHKHSERKSLYSEADALIAVGKG
                     NKESIIEQISKYREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNSESGRDQ
                     LAFPYVLWKSGHAMKEVTTLGNNVYKSAKLRIISHY"
     gene            1397..>1518
                     /locus_tag="ACHAYA_002982"
     CDS             1397..>1518
                     /locus_tag="ACHAYA_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8606701.1"
                     /translation="MKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDV"
BASE COUNT          575 a          177 c          273 g          493 t
ORIGIN      
        1 attataagag aacaaaagta aagaattata tctacttgct tgcgtatttc ttttttatca
       61 ttgtgtgttt gatattcatc aatataagag gaactgcttt tgtaaatgga atcctaccat
      121 tatttagaga aaatattatt tttaaggctg tttactattt ttttgagtaa gaataaatag
      181 cacttaaaaa tagagatgag ggatttatgg gaagacaaga attatatagt ttagctatca
      241 atgaattgga aaaaagaaat cttgaaatac atgaattaaa tcaaaaaaat aattctattt
      301 taggcatgaa aatacgaaat aaaattaatc aacttaacca aatgaaaaaa cgagataatc
      361 tgttgccagc aaaatatacc cttaaatcga tgaccggtaa ggggcctatt aaagactatt
      421 caaacagaga gaatcgcagt gatgaactaa gaaaaaaaga gttaactaat caaggaacac
      481 ctaaaaggat tgctgtgtat agttgtatca ctggtaatta tgattctatt aaagaaccgg
      541 atttttctca tccattggta gattactatt tgtatacaga taatgtgatt acaagtgagc
      601 attggaatat tagagagata cctaaatata tatcggaatt aaaaaataat attttgatca
      661 atagatatat taaatttcat cctaatgagc tatttactga ctacgatttt actttgtatg
      721 ttgatggtaa tgtaacaata acttctgata ttaccggatt actgaacagt tcttccaaac
      781 ttgggttaag tatgcataaa catagtgaaa gaaaatcttt atattctgag gcagatgcat
      841 taattgcagt tggcaaaggt aataaggaaa gtattataga gcaaattagt aagtacagag
      901 aagaaggatt tcctgataat tttggattgg ctgaagccac ggttatttta gttgatttaa
      961 ataattcaat cgctgagaat cttctagatt tgtggtggag agagtatttg aactcagaaa
     1021 gtgggcgaga tcagctcgca tttccttacg tgttgtggaa gtccggacat gcaatgaagg
     1081 aagtgactac cttaggcaat aatgtatata aaagcgctaa attaagaatc ataagtcatt
     1141 attagcccta aatgatttct aaactattct ttcaaatcct agtaaatatt tggagataga
     1201 agatttcctt tttaaagtca taaaaaatta agttatattt tttgagtgaa tggagaagac
     1261 gcatagtatc ataaacttcg ttatatctta aattatgggg tatgcctaaa taataaatgt
     1321 aatacgagta taaagaagaa agcaattttg ggtttaaccc tattggttcc tttcagaaaa
     1381 aggtggatga ataaaaatga aaatattaat aacaggtgga gcaggattta ttggttttca
     1441 tttgtctaga aaattactca aggaaactga aaatcaaatt gtagcgattg ataatctcaa
     1501 tgattattat gatgtcca
//
LOCUS       DAYYCW010000220         1508 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000220 DAYYCW010000000
VERSION     DAYYCW010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.228"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..697
                     /locus_tag="ACHAYA_002983"
     CDS             290..697
                     /locus_tag="ACHAYA_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606702.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            711..1112
                     /locus_tag="ACHAYA_002984"
     CDS             711..1112
                     /locus_tag="ACHAYA_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606703.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1114..1485
                     /locus_tag="ACHAYA_002985"
     CDS             1114..1485
                     /locus_tag="ACHAYA_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606704.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          574 a          185 c          264 g          485 t
ORIGIN      
        1 ttttgagggg aagtacatat agttgttgat atagttgcta atattgttag ctaatttgtt
       61 agtaaaactg tatacgaatt tagatagtaa aaaaacagtt tgttccttat atagaggcac
      121 ttttactact gattaaatgt cagatatgct tacaaatata cttataaata tgcttacaaa
      181 attagctaat atttccgttg acaaagcttt gttactattg ttaaactaaa ttgactagca
      241 aaagtgaaac ataaatttat taaataaatg taaaaattag gagaaatcaa tgtcttatag
      301 agtacaatta ataatttcag aagatgtaga aagtcagcaa tttggcacta atgttattaa
      361 taaagtaatt aatccagctc tttctattaa tgcgccttta attcctactg ctttatcatt
      421 ttctgtaaca gctgtggtgt ctgagataga ggatacagaa aagataaaaa tagtagaaat
      481 tgaagtttta aacaagaatg aaaaacaaat tttttctacg ggtgaagtat cggtaagctt
      541 gcctcctcaa gttaacgata taaactttaa tataaacgcc agaaatgtct tggttgaaga
      601 agcaggagag cattatgctg ttgttaaatt taatggaact gagattggaa ggcaaatctt
      661 tgatatcaag gtcaacaaac cagtggaaaa aaattaagga gacgatacgg atgcttgata
      721 tagtaccatc aaatacacat cggagtggta gcttaataaa ttttgctgcg atttttgctt
      781 gcgcatcggt aattctagca tctccagttg ttgtggatgc aagttctact ccgaaaacta
      841 aaaatgataa tcaacttgta attactacaa aaaatgagat taacacaatt tcaagtaatg
      901 atggcaaaat ttttgatgtg ataaatacgg ttttaaaaag aaaagctgaa atcaatataa
      961 aatatgatga agatttaaac ttgtatttct ttgttatcaa aacaacatct gaattgtttt
     1021 cctcagatta cgatgttttg gatactctcg ataatgtatt atctgactat aaatatatgg
     1081 ggaaatctgt agtagcaact ttgggggaat aaaatgtttg actttaatga atatttatct
     1141 atttccaaac aaattgaatc agatgataaa tataattcta aagaatctta tagaagaact
     1201 gctgtttcaa gagcatacta cagtgctttt aaaaaatctg atgagtattt gaaagaaaat
     1261 tatgatatta tttataatgg aagtagcgga aaaggaagcc accaaaccgt ttggaatttg
     1321 ttttcaacag ttaaggaatt gaatacctta ggaatacata atagtgggta tagaatgtta
     1381 gagaaaagaa aatgtgctga ctatattcct aatgaaacca ttacgaaaac agatatggcg
     1441 ctgatgaatc gagaagcaga aaaaataata aacaaactca cctaatgaat ttcaagcgag
     1501 tttgtttg
//
LOCUS       DAYYCW010000221         1504 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000221 DAYYCW010000000
VERSION     DAYYCW010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.229"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..993)
                     /locus_tag="ACHAYA_002986"
     CDS             complement(37..993)
                     /locus_tag="ACHAYA_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606705.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1118..>1504)
                     /locus_tag="ACHAYA_002987"
     CDS             complement(1118..>1504)
                     /locus_tag="ACHAYA_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8606706.1"
                     /translation="DRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLI
                     ENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDT
                     YNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          470 a          245 c          241 g          548 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aacttgggag cgtcaataat
     1081 tttgtgtaaa taaattgtcc tcctgcaaaa taattagtta ctcagtaaac attgaaacta
     1141 atgtatcggt tacctgttga aaacctttat ggcttctgtt tagaaatttt tgattgtatg
     1201 tatcaaaaat gctgactaga aagcgttcta gtgattcttc attttgaaac tgctcttttc
     1261 tacggctgta tcttttaatt tgcttattga aagactcgat tagattggtt gagtaaatgg
     1321 ttctacgaat gctaggtgga aaatcataaa aagttaataa gtcttggttt tctatgagtg
     1381 actgcgtcac tttaggatag tttttcttcc atttctcaat catgccggat aagaaggtat
     1441 tcgcttcttc ttttgagtta gcttgataaa cagccttaaa gtcatcacag atttcttttc
     1501 ggtc
//
LOCUS       DAYYCW010000222         1480 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000222 DAYYCW010000000
VERSION     DAYYCW010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.230"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACHAYA_002988"
     CDS             64..972
                     /locus_tag="ACHAYA_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8606707.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="ACHAYA_002989"
     CDS             972..>1480
                     /locus_tag="ACHAYA_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606708.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DAYYCW010000223         1464 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000223 DAYYCW010000000
VERSION     DAYYCW010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.231"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(315..398)
                     /locus_tag="ACHAYA_002990"
     tRNA            complement(315..398)
                     /locus_tag="ACHAYA_002990"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Leu,seq:cag)
     gene            926..1426
                     /locus_tag="ACHAYA_002991"
     CDS             926..1426
                     /locus_tag="ACHAYA_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8606709.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
BASE COUNT          466 a          252 c          236 g          510 t
ORIGIN      
        1 tttgaacgtt taaacacatt caaaatatag taagaggtca tgcgatttat tctttcgcat
       61 gacctctttt tgagtttaag tttagtcttt attccttaac tatcgtttgc cagtatttct
      121 tgtatcagaa tctataaatc tactatttga atcaatctat taggttttaa actgtcccac
      181 cttcacacaa attttttttg atttatttga atctggttcc gttgtttaaa atttttccta
      241 tattctttta gataatatgt tattttcttt ctcatcgctc caggtgtacc ttctctttat
      301 aaaaaaacaa attatgcggc taagaagact cgaacttcca cgaggtcacc ctcactagcg
      361 cctgaagcta gcgcgtctgc caattccgcc atagccgcat cacatctttt agtttaggat
      421 tacatgtgtt ttttgtcaag aacatatttt tgttcttttt catttctgta cgccctccct
      481 tatgccatct acacaaataa ttcatccgag caactccatc atctattgag ggttggtgaa
      541 aaaatctgtt ttatccatat gatcgatttc aatgttaaac gaacgactct gggattagta
      601 actattttat ttttttcttc atttcttttt tcctcttttt ctcgattcag ctatcttctt
      661 tcatttggat attgattatt tcttctgttg attactatcc ttctggaatt ctagacgctg
      721 aagtaattca cgttttcttt cttctagaga tcataagtaa cgaatgaacg aacttcaaat
      781 aattttttta ttgtaattca tatttgtatt gttcgatgat tcttaatacg atatgacaca
      841 ataaataaaa ataggcatgg ctatttacaa caatagtgct atagtagaaa acaaatgaat
      901 aaggttactt aaagaggtga atacgatgaa actgatcaaa ctatgggaag cagatttaaa
      961 gaaagcttat cagctacaaa ctagctttgg accaaatgag agtggattta tcaacaatgc
     1021 ttatggctat acatttgctg aatttttaga ttatgtaaaa aaatgcgaaa aaacgtcttt
     1081 aggaatcgat cttcctgaga gttacgttcc cgcaaccgtc tttattttag aaagtgatga
     1141 acatgagtat gtaggaattt ttaatttgcg ccataaactg aacgaaacat taagaaacgg
     1201 cgctggccat attggttacg gcatctcccc taattatcgt aaaaaaggat atgccacaca
     1261 aggattagca ttagtactac aagaggcaaa aaaaatcggc attgatgagg cacttttgtc
     1321 tgtaaataaa aaaaatcttg gcagtcttaa agcacaacaa aaaaatgggg ctgcgattta
     1381 tcgtgaagat gagaaagaat actatacgaa aatcaaactg atgtaaaaaa gaaggattcc
     1441 aaaaggaaag ggatttgaat gtcc
//
LOCUS       DAYYCW010000224         1436 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000224 DAYYCW010000000
VERSION     DAYYCW010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.232"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..342
                     /locus_tag="ACHAYA_002992"
     CDS             <1..342
                     /locus_tag="ACHAYA_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8606710.1"
                     /translation="VYFSVVSARRNPYFNMVKRIENEVTIVNKSRYTSRQQTPEVFEM
                     NASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDIDSEEDFKWLEFLFPKFLEDSSE
                     LQDIYNYLYRR"
     gene            347..1294
                     /locus_tag="ACHAYA_002993"
     CDS             347..1294
                     /locus_tag="ACHAYA_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8606711.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          477 a          203 c          295 g          461 t
ORIGIN      
        1 gtctactttt cagttgtttc tgcaagacgt aatccctatt ttaatatggt aaaaagaatt
       61 gaaaatgaag ttactatagt taataaatca agatacacat ctaggcaaca gacacctgaa
      121 gtttttgaaa tgaatgcctc aatgtattct tttaaacctg aatttctaaa aaaaagtaat
      181 tatatttttg atgggcaatg tgggataata aaaatgagag attatctaat tttagatatt
      241 gatagtgaag aagattttaa atggttagaa tttttgtttc ctaaattttt agaagattct
      301 tcggagcttc aagatatcta taattattta tatcggaggt aaaaggatga gctgttatct
      361 tatcaccggt ggggctggct tcattggttc taccttagcc aatcatttag gagaagaaca
      421 tgtggtaatc gtcgtcgatg acttatcaat gggaaaagta gaaaatcttg atatgacgaa
      481 aaatatcact tttattgagg gagatgtcgc tgagaaatcc ttgatggaag agatcatgaa
      541 agctaataaa tttgattata tctttcattt agcagcagtt gctagtgtgg cagattcagt
      601 tgcccgacca gtagaaacac atcgtgtgaa ttttgaaagt gtattaatga ttttagaatt
      661 gatccgcaag tatcaacctg atttaaaacg gatcgtgttt agttcttcag cggctgttta
      721 tggggatgaa ccgactttac caaaaaaaga agagtccgtg attcgcccat tgacgcctta
      781 tgcggttgat aaattcgctg cagaacaata tgtgctagat tattgtcatt tatatgatgt
      841 acccggtagc gcagttcgtt ttttcaatgt ctatggtcca aatcaaaatc cgaattctcc
      901 atacagtggt gtgatttcta ttctcgtaga tcgctataag aaacaaatcg taggagaagc
      961 aacttcattt acattatttg gtgatggaag ccagtctaga gactttgttt atatagatga
     1021 tgtaatccaa gcattacttt tagttgcgaa tgaggaagct gcactgggga aacaattcaa
     1081 tgttggaacg ggtaaagcaa ccacactttt ggaattgatt catgcaatca atgaaattct
     1141 cggtgtagaa ttgacacttg aatacaaaga agagcgttcg ggtgatatcc atgactctct
     1201 agcagatatt actaagatcc agtcaattgg ctatcaaccg aaatatggta ttttaagtgg
     1261 aatgactaat tatttgaaaa ctgaaatcaa ttaaaacata ctaagataag attagagggt
     1321 aaattcaggc tctttgtcaa taaggactga tgagttttgt aaaatcaaat ctgggataga
     1381 agggctcttt gtcaaatgat gttggtaaag agaaatctta gaataatttg gacaga
//
LOCUS       DAYYCW010000225         1433 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000225 DAYYCW010000000
VERSION     DAYYCW010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.233"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..458
                     /locus_tag="ACHAYA_002994"
     CDS             48..458
                     /locus_tag="ACHAYA_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8606712.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            472..828
                     /locus_tag="ACHAYA_002995"
     CDS             472..828
                     /locus_tag="ACHAYA_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8606713.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          530 a          209 c          268 g          426 t
ORIGIN      
        1 aaagaaatta ctgtagaatt ggattataat taaaaaggag tgagaaaatg ataaaagtat
       61 atacttcgcc tagctgtact tcctgtagaa aagcaagagc atggttgcaa gcgaatcaac
      121 ttgaatttga agagaagaat atttttgccg aaccgttaac agaaagtgag atcaaaaaga
      181 tcttggcagc gactgaagga ggagttcaag atattatttc cactcggtcg aaaatttacg
      241 aaaagctggg catagatttt aacgaattaa ctttaaagca aatgatcgtc ttattcaaag
      301 aatatccttc attattgaga cgacctattt tgattgatga aaaaagaata ctaatcggat
      361 ttaatgaaga tgagatccgg tcatttgtcc ctcgtgaaat tcgatgtgtt gccagaaatc
      421 tgatctattt acaaaacaat aacatcttaa attcataggg agggagcctt cgtggaagtg
      481 aaagtctatg gatcagcgca ctgtgtaaca agtctgaagg cgaaacaatg gttagaacac
      541 catcaattgc cttatcaatt tgttgactta gaagaacgcg atttaagtac agaagaagca
      601 gaagaattat gcagttttga agaagtgcaa gcagaacagc tatttgcgac ttggtctgag
      661 gcttttcaca acattgcgat tgacctttcc tgtgtaggaa aaaaacaact gattttttta
      721 tgcacacaac ataccaatct cctcagacgt cctttgatta ttatcgatga agttctattt
      781 attggataca atgagcagtt gatggaacag cttatcttaa ataaatagac tttaactgtt
      841 ttaatcaagt cactgagcat ttcatacagt tgtaacagtc gaacacttag catcaagcgt
      901 gttatgtgtt ctttttttga ctttattttt ctactataga gacatgctca atttatgtgg
      961 tcaaaattca aatctaggtg gggatataga gttaaaaata tattgacatg tgtaaattta
     1021 catgatatat tcatttctgt tgttttaagt agcaaagcaa taaaatagta ttttaaaaaa
     1081 catataaaat agttgttgac atgttagaaa tgacatgata agataactga gttgttaaaa
     1141 aagaaccact tgtgaagctt gattctatcg aaaaaaactt caaaaaagtt cttgacaaat
     1201 aacaaatgat aagttatgat aaatgagttg ctgtaagatt tcgaaaaacg aaatcaaaaa
     1261 aacttttaaa aaagagttga caattacttg tctctctgat atgatataaa agttgctacg
     1321 gcagccaaaa acgaatcggg gaacactcct gatgaagtag acctttgaaa actgaacaaa
     1381 gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa aca
//
LOCUS       DAYYCW010000226         1364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000226 DAYYCW010000000
VERSION     DAYYCW010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.234"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1364
                     /locus_tag="ACHAYA_002996"
     CDS             82..>1364
                     /locus_tag="ACHAYA_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM8606714.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAYYCW010000227         1355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000227 DAYYCW010000000
VERSION     DAYYCW010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.235"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACHAYA_002997"
     rRNA            <1..1355
                     /locus_tag="ACHAYA_002997"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAYYCW010000228         1338 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000228 DAYYCW010000000
VERSION     DAYYCW010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.236"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1338
                     /locus_tag="ACHAYA_002998"
     CDS             <1..>1338
                     /locus_tag="ACHAYA_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HFM8606715.1"
                     /translation="LLDFLLNYRFSENVGNLKNIIQLTCANAFYFLDDLEELYIQMYH
                     LPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEYLYQTWKKDTDKQQLFE
                     KYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQIEFDYYYLQITSIFLNS
                     WIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYLTKIQKNVLVILFKLMC
                     SKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMPLMTSSSEIADKINRYL
                     QECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTRMAIDVANQILLDLPLK
                     QILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKRILEDSFPRDTDLVIET
                     CDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIEDMILGMNIRKFSN"
BASE COUNT          494 a          166 c          229 g          449 t
ORIGIN      
        1 ttattagatt ttttattaaa ctatcgcttt agtgaaaatg ttggcaattt gaaaaatatc
       61 attcaattga cctgtgccaa tgccttttat ttcttagatg atttggaaga actttatatt
      121 cagatgtatc atttaccaca atgggtgaat ttcaacggca tgaaggttat gaaaagacag
      181 aatgaattaa aatatattca aatggatgat ttggaaaaag tagacgaagt ttttaatatc
      241 aacgaactta tagagtatct ttatcaaacg tggaagaaag acactgataa gcagcagctt
      301 tttgagaaat atgatagata ttttagacaa aagaatgttg atatccaaaa taattcagct
      361 ttaacatatc ccacctatca agagattcga tttagacagt atgttaagga aatagagaga
      421 gattttcaaa tagaatttga ctattattat cttcaaataa cgtctatctt tttgaattct
      481 tggatttatc aaatccaatt aacatcggac gataaattat attccttcat caatgatatt
      541 aatagttggt gtagtcgaga agttcaatta gtcaatcgtt ttattcaaat aggggatatt
      601 tatttaacaa aaattcaaaa aaatgttttg gttattttat ttaagttaat gtgtagtaaa
      661 gaatttcagc aaaaacgttt agcactgatt ttggctcatg gagatctaac ggcaacctgt
      721 atagcaagta ctgctaatag gatcgttgag agatatatat acgacgccat tgatatgcca
      781 ctaatgactt caagtagtga gattgcagac aaaattaata gatacttaca agagtgtacc
      841 ggatatgaag aaattatttt acttgttgat atgggttcac tggagaaaat ttatctaaat
      901 ttggaactag acgctcaaaa aaaattcgga ctactaaaca atgtttcaac aagaatggct
      961 attgatgttg caaatcagat attattagat ttaccgctta aacagattct tgaacaagta
     1021 aaagaaaatc aaaatgtgga ttatatttat gaatcatcaa atattaagaa ggatatgatt
     1081 gtctgttctt gtgcaagcgg attaggaact tcgtttaaat taaaaaggat acttgaagat
     1141 agctttccaa gggatactga tcttgtcata gaaacgtgtg actattactc tttagtgaat
     1201 gaaaactatt tatcggagct tcgtaaagag aacaatattc tttttattgt tggaacacta
     1261 aatccacatc tagaagatgt agaattcttt tcaattgaag atatgatttt aggaatgaat
     1321 ataagaaaat tctccaat
//
LOCUS       DAYYCW010000229         1324 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000229 DAYYCW010000000
VERSION     DAYYCW010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.237"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..290
                     /locus_tag="ACHAYA_002999"
     CDS             <1..290
                     /locus_tag="ACHAYA_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606716.1"
                     /translation="SWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(283..417)
                     /locus_tag="ACHAYA_003000"
     CDS             complement(283..417)
                     /locus_tag="ACHAYA_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606717.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            589..>1324
                     /locus_tag="ACHAYA_003001"
     CDS             589..>1324
                     /locus_tag="ACHAYA_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8606718.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETS"
BASE COUNT          519 a          205 c          227 g          373 t
ORIGIN      
        1 cttcatggga taacgaggag tattatcttc ttgtactgaa tcaattaaca tcgattgaga
       61 ttcaattgtt agcatgctta atgagtcgtg gaaacgaaaa tttcactgga aatatcagca
      121 tgtctggcta ttcacaagag ctaatagatg gtagcttaaa tagattatct gattttggtc
      181 tgttagaaaa aacgattagc tccatagtat taggtgatgt cggcaaacaa aatatgggct
      241 acaaaatatc tcatttagga attcaattta ttaactcaac tcttacttaa gttttctttc
      301 taatatgaca tctagaccta tcagcaatgc aacaataaaa aaccagacat accaataatc
      361 aacaaataat ctaaactctg gaagttcagt tattctttta aaaatcactt taatcataat
      421 aaacacctct gattggatga tctaatacac atccaattat accattaaaa cgacaaatag
      481 cacatccttg cgccggcaag caaaaaggat gtgcaaaaaa taataaaacc caaacaatgg
      541 gcttctttat agttcctatt gtatcagaga aagagagctg attcaattat ggcaagatta
      601 gtcaaacgtg gaaatagttg gcaatacgaa atttcataca aaaaagatga cggaaaatac
      661 acgaagataa gaaaatcagg atttaagaca aaaggagaag caaaagatgc cgccaacgaa
      721 ttagaatata acttgaacaa aggccttaaa ggggatcgca aaaatctatt attatcagat
      781 tactttgagg attggatgca actttataaa gaaggaacag tatctcctat cacttataga
      841 aaatacgaag atacgttaat gaacataaag aaatatatgc cagcggtatt gatttctgat
      901 ttagatagag tgggatatca acgcttttta aataagtatg ctaaagacca tgtaaaatct
      961 accgttatta agtttaataa ccatattaga gcgtcgttga aagatgccgt agaagaagga
     1021 ttaattccgt ttgatccaac tagaaaagca gtaatcaaag gaaaagattc attgaagcca
     1081 aaagaagata aatatttaga ttatgatcaa tttaaatctt taatgaaact cgtagaagaa
     1141 aacctttctg cgcagtactc ttctcctatg ctcgtgttag ttgctggtgc cactggaatg
     1201 cgatttgctg aacttctagg attaacatgg gaagatatcg atttcgaaga tcaaatcatc
     1261 actattaata aaacatggaa ttataaatta aatgaatggg gaaaaacaaa aaacgaaact
     1321 tcaa
//
LOCUS       DAYYCW010000230         1281 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000230 DAYYCW010000000
VERSION     DAYYCW010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.238"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1281
                     /locus_tag="ACHAYA_003002"
     CDS             <1..>1281
                     /locus_tag="ACHAYA_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8606719.1"
                     /translation="KPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTEIVD
                     SKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFGVYF
                     GVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGNQPI
                     GKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELDGAF
                     GLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNWIFD
                     FEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFLLVS
                     ICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSDCLL
                     EKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHL"
BASE COUNT          452 a          190 c          231 g          408 t
ORIGIN      
        1 caaagccaga gatccaaatt tcagaagaag cagagatcca tgtcgaacgt ctgaatttga
       61 cggatatcaa aaatttaaga agaatctact ttcaacgatc aaaaattgct cgactgttaa
      121 cggagatcgt ggattcaaaa gtaacgatcg aaaaatttgc agagaatcat tttttaagca
      181 tatcaagagc ctatgtaaaa agaagagaat tagtctcttt tcttgaacag catcaaataa
      241 agttaaagaa aaacatatta gttggggaag aaataaacat acggaacgta ttatttgggg
      301 tatatttcgg ggtctttaat ggtttttcta tgccatttag agaaagcgtc atgaatgaag
      361 caaagagtat cgtgcaatat tttgaatatc tgttcaattt aaaactgtct gcaacaaaac
      421 gaatcaaatt caatttgcta accactatta gtttgttgag ggcctatggg aatcaaccaa
      481 tcggaaaacg tttcttttca gatgaatact ttaagagctc agaaggaatg aagatcatca
      541 aggatttatc acatatttct tttcttcata tccctaaaga gcagatcatt gatgagattt
      601 cttatatcat gttgtttttg atcacggaag aagaatttga attagatggc gcatttggac
      661 ttgcggaagc tcattttcat gatatccaca tgattagtca aaaaatgtca gtggaattga
      721 ttgatcaatt accattttcg attgaaatct ctgaagaaga aagactatat atacgaaaag
      781 aacttcgtat gggtatgatg catgtcaatc gaaaaaattg gatattcgac tttgagatga
      841 ctagctttac gtctaaaaag cagctacagt ttttcttcga gacgtatcca atattcagta
      901 cagcaatcca aaaggtcata caagagaata aacgctattt cttgggaaaa gatgaacgtt
      961 tcatgattcg cttattttat gattatctct ttttattggt cagtatttgt ccggtatcat
     1021 attttgaagc accgattcat gtctgtattg atttttcgca aggatctgct tataccgatt
     1081 acattatttc tcaagtgaaa ggatttaaaa attacaatat tgtgattgag agtcgaatca
     1141 cttctcgtac gaatatttat ttatctgatt gtttacttga aaaatttaat aaagaacaga
     1201 taatttggaa aaatccaccg actccagatg actgggaata ttttggaaat accatcatca
     1261 gagaaaaaaa tagacattta a
//
LOCUS       DAYYCW010000231         1277 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000231 DAYYCW010000000
VERSION     DAYYCW010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.239"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1265)
                     /locus_tag="ACHAYA_003003"
     CDS             complement(<1..1265)
                     /locus_tag="ACHAYA_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM8606720.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKV"
BASE COUNT          499 a          213 c          149 g          416 t
ORIGIN      
        1 actttacttc taaaaaatgt tttgagtaaa ctaaagatat ttaacacata gaaagcatag
       61 atacatataa aagcaattat accacttgat acaagtactt gaagaaatcc attatgcatg
      121 tttccatgtg ctctttttag ttcattcaca tctgtagtat caagatctac ttttttcaaa
      181 aattttgcat cgctattccc atagaaatcg gtctctccat aaccaaataa caaagttggt
      241 cttataactt taagcgcagc tttccaaatc gtaaaacgac cagctgaaat ttcggagtta
      301 tcactagaat gctgtaatat tacttctttt tctggaagtg gagttttatc tgattcattt
      361 gactctataa cttctacttt tctatcaatc gatacaggaa ttgttgctaa aaaatcctta
      421 gcataattag taccaaaaga aaaatatacc aatagaacta ataatatgct taccatccat
      481 ttgattttta caggtagtac ttctctaatg ctctttctaa attttgaatt ggcaaacaac
      541 aaaagaaata atactaaaaa agaacaaatt actacctggg cacctctaga cgaagaaagt
      601 gtaatataaa atatttgtac gataaaattc atcatataaa aaacacgaat cgtttttgtt
      661 atttttgaaa ttaatacact aattaaactt aaaataatag aagtatatcc aaacatagct
      721 ccaacatttg gacttgtata gacaccaaat aaccgatgtt caataaaacc ttgtcttgca
      781 agtaatcctg gcgttcctgt tgggacatga taagaaatca aaaaaataaa ttgatagata
      841 gatattagcg cagctaaaaa aattatgata atgaaaacat tattaaaata caccattttt
      901 ttcttttttt cctcttcact ttgtgaaaag tctgcaggaa aaattactat caaagtaatc
      961 agcaaatatc caaaattata aatagatgga attaaaaaat tttctctgtt aatgattata
     1021 gttaagataa aactaatcat tgctccaatc agtagccaag aaaaacgcat tttaaaaaaa
     1081 tttttcttta taaaaacctc atacaaaact ataattccac cccaagcaaa agctattttc
     1141 atcgggatat tagttacatt aaaagtacta ggcataaaat aaagcagaca aatgattaaa
     1201 tttattactc gaaacgcatc tcgacttaat aaactcttct ctaaaacagg tactattcta
     1261 ttcatctata tttccaa
//
LOCUS       DAYYCW010000232         1249 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000232 DAYYCW010000000
VERSION     DAYYCW010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.240"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..284)
                     /locus_tag="ACHAYA_003004"
                     /pseudo
     CDS             complement(60..284)
                     /locus_tag="ACHAYA_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACHAYA_003005"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACHAYA_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          443 a          212 c          170 g          424 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactac
//
LOCUS       DAYYCW010000233         1173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000233 DAYYCW010000000
VERSION     DAYYCW010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.241"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..105)
                     /locus_tag="ACHAYA_003006"
     CDS             complement(<1..105)
                     /locus_tag="ACHAYA_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase"
                     /protein_id="HFM8606721.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFV"
     gene            104..952
                     /locus_tag="ACHAYA_003007"
     CDS             104..952
                     /locus_tag="ACHAYA_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8606722.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT          403 a          204 c          197 g          369 t
ORIGIN      
        1 tacaaaaata ttttttgttt gattttttcc tactctcctt gtatctttaa cattaatgag
       61 aacggaaaaa atgataatct tattatgaaa atgaggaatt ctcatgagcg tctccataag
      121 actacaaatc aaaacaatgt acaacgactt cagcccaaaa gaacaggcta tcgccgatta
      181 tattttagaa aatccaagca aagtaagtca tagtcctatt agcaatttag caagtgaact
      241 gggcattgct gactctactt tttttcaatt tacaaaaaaa cttggttata atggatttaa
      301 agattttaaa atggccatgt tgatccaaga aaatgatcta tctgctattt ctatccacga
      361 aaatattcag aaaacagata ctgagctcac tatggcgcaa aaggtgttcg agtctagtat
      421 ttcaacttta aatgacacaa aaaagttgtt acaacaaaag gatctaaaaa aagcggctac
      481 tattatcaat gaatctaaac gtgtctattt tttcggtgtt ggcggttcag agattgtagc
      541 aactgacgct tatcataaat ttttgcgttc ccctatcgca acttctcaca gcacggatta
      601 ccatattcaa ttgatggaag cttctttatt gacggaaaaa gactgtgctg ttttgatttc
      661 tcacacaggg caatcaaaag aaacaatcca tattgctgaa accgtaagaa aaactggagc
      721 aaaaacaatc gttattacta gccaagctaa ttcttccctt gctaaacttg gcgatgtcgt
      781 ttttatttct atttctgaag aaacagaatt ccgatctgaa gctttagctt cccgtatttc
      841 tcaactaagc attttggact ctctatatgt gattttgatg ttttacaacg aaaaagaatc
      901 acgtcgttct atcgctaaag ttcgacatac tatttctgaa atcaaagaat agcaccataa
      961 cagaaaaagc gaaaaatcca taaaacaaaa aaagccacta ccaaatattt ggtagtggct
     1021 tttttggttc tgcaagggtg gtacagaaca tcaatagtcg gtgggttaga acgtatgtca
     1081 tcgcatcttt ggagaaggtt gtattggtat tattcgagtt acttgttaga tgcgataaca
     1141 aagttagtat acagtagtaa aaaatttatc ttt
//
LOCUS       DAYYCW010000234         1119 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000234 DAYYCW010000000
VERSION     DAYYCW010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.242"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..404)
                     /locus_tag="ACHAYA_003008"
     CDS             complement(<1..404)
                     /locus_tag="ACHAYA_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8606723.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHS"
     gene            755..1054
                     /locus_tag="ACHAYA_003009"
     CDS             755..1054
                     /locus_tag="ACHAYA_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8606724.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          407 a          159 c          185 g          368 t
ORIGIN      
        1 gagtgctcaa aggtactttc ttttatgttt gaaccagata aatttacata gtccatttta
       61 caattctcga ataacgtgtt cccaatataa ctttccatca atttagtacc tgttaattta
      121 caattgacaa atttcacgcg atgcaacatg ctttttgtca atatacagcc tgaaaaatca
      181 cagttttcga atatacagtc ggtaatctcc attctttgaa aatcattgga agcaaaaaat
      241 gcatacttga aaatagattt gtaaataatt ggatggcaga ttatttggtt ttcgatgacc
      301 tctttttgtg aaaggatatt atacagttca aaatcttcgt tttcatagat atgaaaaaag
      361 gatcctttat tcaagcttgc ttcattaact ttaggaaaaa tcatatttaa ttacctccat
      421 tttcataaag tcatattaac aaaagctaaa ccaacttaca ttagcagaaa atgagatagg
      481 ttttcgcctt tttcgataac gatagtgtcg ctatctcttg aaacgttctt agaattgccc
      541 tgagagacac aaattaggcg taattctaat aaaaatctta gaataaaaat ttttactgat
      601 ttaatttaga atgtttttaa aataaaaatc gttgccataa cagtgtattg aaaggtttta
      661 acaatccagt aatgatttaa aaaaatctag atgtagcgta tactttgttt atagaatgta
      721 gtgggaagaa atatattttt gggaggaaat cgtgatgtct aaaagtgaag aaatagaaaa
      781 catggtacta aaattatatg atctcgtttt aaatccagag atcaaagaaa aggaacgtat
      841 acttttgata gacgcaaaga caggcttaga aaaaggtcag tattatccta aagtcataaa
      901 taatttggaa cgtagtttac gtccgttagc aatcagggga gaattgtcta aaccagtatc
      961 accgttttat atggagatat caacaatagg aaaatttgaa aaagaattag gtcgaggaat
     1021 ggcttcggca ccgattactt ttgggcattt ataaaaacga gttattcatc tcaatgaatt
     1081 aaaaagtata gtcaatctca taatgcaggc taggttcat
//
LOCUS       DAYYCW010000235         1069 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000235 DAYYCW010000000
VERSION     DAYYCW010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.244"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACHAYA_003010"
     CDS             <1..969
                     /locus_tag="ACHAYA_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8606725.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DAYYCW010000236         1035 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000236 DAYYCW010000000
VERSION     DAYYCW010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.245"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1035)
                     /locus_tag="ACHAYA_003011"
     rRNA            complement(<1..>1035)
                     /locus_tag="ACHAYA_003011"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DAYYCW010000237         1020 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000237 DAYYCW010000000
VERSION     DAYYCW010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.246"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACHAYA_003012"
     CDS             <1..459
                     /locus_tag="ACHAYA_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM8606726.1"
                     /translation="LLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSV
                     IYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFM
                     KVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            468..695
                     /locus_tag="ACHAYA_003013"
     CDS             468..695
                     /locus_tag="ACHAYA_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8606727.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          318 a          166 c          180 g          356 t
ORIGIN      
        1 ttgctattaa aatttgtcgt tttaggtata agtgccgtat ttattttgtt cggcgtactt
       61 ttttatactc agatcgctac aagcgaaaaa ggattcaatt ttgattggca gaccacatta
      121 tttgtttctg tcatttactt tgcccttctc ctagggatcg ttattgctta tttcttattg
      181 aaactattga aaaaaatcga agataaccaa gctttttcca ctcaaagctt aaacatacta
      241 aaaaaaatcg aacgaaccat tttgtttatt ggtttcgcct tttttggctt acttcctaaa
      301 tttatgaaag tagcggattt agacgatgct ccaggtttga tgttggtagg aatcgctgtc
      361 gtattcattc cttttgcagt atacacatta acaagcgtgc tggaaaaact gcttgaacat
      421 gcgattttac ttaagacaga tcatgatttg acagtgtgag gtgcgacatg gcaattatcg
      481 ttaatttaga tgtagtatta gctcaaaaaa aaatgagtgt gactgaattg agtgagaaag
      541 tgggcattac gatggcaaac atctctattc taaaaaatgg aaaagcaaaa gccatccgtt
      601 tttccactct cgaaaaaatc tgccaagttt tggagtgtca accaggtgat ttgttgacct
      661 ataaaaatga ctcgaatact ataccagaag aataaaataa agtagaacaa acagtaaata
      721 aaaaataatc tgaatgatag atgtgtgaga cttttagaag ttatttacgt ctattattca
      781 gattttttat ttcgattgaa taccgtttta tctataaata agatatgtac tgactcatac
      841 aaagggggcc ttttcttctc catgtactat cacttttcct gatacaactg gtttcctgtt
      901 acgttgtagt tggatctgct tttagtataa tactttattc attccaccag actgatagca
      961 gtttgagcag tctaaataat catgtagtat tacaggccat tctgtttcat accttgaagt
//
LOCUS       DAYYCW010000238         1001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000238 DAYYCW010000000
VERSION     DAYYCW010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.247"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            369..1001
                     /locus_tag="ACHAYA_003014"
                     /pseudo
     CDS             369..1001
                     /locus_tag="ACHAYA_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac c
//
LOCUS       DAYYCW010000239          998 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000239 DAYYCW010000000
VERSION     DAYYCW010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.248"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..606
                     /locus_tag="ACHAYA_003015"
     CDS             <1..606
                     /locus_tag="ACHAYA_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM8606728.1"
                     /translation="NMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDI
                     IEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIG
                     LNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTIS
                     EVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            623..>998
                     /locus_tag="ACHAYA_003016"
     CDS             623..>998
                     /locus_tag="ACHAYA_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606729.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTF"
BASE COUNT          360 a          109 c          182 g          347 t
ORIGIN      
        1 aacatggttt ttaatctgag taatggaata attccaagtg ttagtggaga tacgattcgg
       61 tctgaaaaaa attattctat cattgttttt gaaaagttag cacaaacgtc tataacatta
      121 ggaatggata taatagaagc gtatcaaagt agagatgctt tgatacaaga aaacgaatta
      181 gctgtatcgt tacctgaggt cttaaaagtg agggattctg ggattgttta ttatactaag
      241 gaaatcggaa aaactaagat agagcatctt tctcccttaa tttcttctgt cgttcaattc
      301 atcggcttaa atatctataa aagaattact gtaaaggaaa tagctaatta tttttctgta
      361 agtgaaacta aattaagaga aagctttaag aatgaaatgc atattacgat ttataattat
      421 atctctaagc gaaaaatttc tacggctaaa ataatgttga aatcaaacca tactatcagt
      481 gaagtttctt taggtttagg attttcagat tcttcacact tttcgagagt attcaaaaag
      541 tacgcaggtg tatctcctaa gcaatatcaa ttaggtttag tggacaattt caacaacatt
      601 agctgagagg ttttatttta ttatgagact atatcattta cgtagtttgg aggagttgag
      661 gaaattggat tttaacaaaa aatttttttg ggaaatttct gatttgagtt gtttcgtaga
      721 atctgagcgt ctaagtaatg gtaataatag tgtgttaaat gaatatgaca aagtagtaat
      781 taataaagta aaaagtttat gtgaagaagc tagagaatct atcagtcata ttaatggtag
      841 caattttaaa caaacattat ttaatctgat taagctagat gctaaaatat caagttattt
      901 attctttttg atacatgatg aatttatgga ttatcaaaaa ttggatcagt taattgaaag
      961 agaaagctgg gaagattatt acgtgggtct aacttttt
//
LOCUS       DAYYCW010000240          984 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000240 DAYYCW010000000
VERSION     DAYYCW010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.249"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>984
                     /locus_tag="ACHAYA_003017"
     CDS             <1..>984
                     /locus_tag="ACHAYA_003017"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8606730.1"
                     /translation="EPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITDS
                     EYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQLD
                     DTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTGK
                     KNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVGS
                     IAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFYE
                     NFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKMF"
BASE COUNT          374 a          159 c          189 g          262 t
ORIGIN      
        1 gaaccaggcg attttatcat gagtggcgct ggaacaattg ggagaatttc aagagtacca
       61 aagcaaataa aaccgggtgt attcaatcag gctttgatta gacttagaat aaataaagaa
      121 atcacagatt cagaatattt tattcaattt attcgtgcag attttatgca aagaaaactc
      181 acaggagcta atccaggttc agcaattaca aatctcgtac caatgtcaga agtgaaaaaa
      241 tggatagtac aatttccaat attagaagaa caaaagaaaa ttgggaactt cttcaaacaa
      301 ctggacgaca ctatcgctct tcaacaacgt aagctagatt tattaaagga aacgaaaaaa
      361 ggattcttac aaaaaatgtt ccctaaaaac ggagcaaaag tgccagaaat tcggtttcct
      421 ggatttacgg aagattggga acagcgtaag ttcaaagagt ttagtaagaa gactggaaaa
      481 aagaacacga aagatttaga cttccctgcc tattcggtaa gtaataaagc aggtttaatt
      541 tctcaaacag aacagtttga tggtagtagg ttggatgatt tagaaaaaac aaattataaa
      601 ttcgttgaac caaatgaatt cgcctataat ccagcaagag taaacgtagg atcaattgca
      661 tttaacaatc ttggtatgac ggtcattgtt agctctcttt atgtagttgt gaaaatgagc
      721 gaggacttag ataatgagtt catacttcag tttatcaagt caccaacatt tatcaaagag
      781 gtcaagcgca acactgaagg aagcgtacga gaatacttat tctatgagaa tttcgctaat
      841 atcaagtttc cttttactag gaataaagag gaacaacaaa aaatcggcgc tttcttcaaa
      901 caactcgacg acacgatcgc tcttcatcaa cgtaagttag atttgctgaa ggaaacgaaa
      961 aaaggcttct tacaaaagat gttt
//
LOCUS       DAYYCW010000241          980 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000241 DAYYCW010000000
VERSION     DAYYCW010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.251"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..>980
                     /locus_tag="ACHAYA_003018"
     CDS             120..>980
                     /locus_tag="ACHAYA_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606731.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTL"
BASE COUNT          331 a          121 c          136 g          392 t
ORIGIN      
        1 aaacactact tttgtaatat aaaaaatatt ttattaataa taaaattaat cacattttat
       61 atagtgacta atctagtgtt taaaaaaaat aaatgatact atatgtaata taaaaaaata
      121 tgaggtggta tatgaatttg aaaaaaattt tttctgtaaa aatatttata gtttatgcat
      181 ttgttagttt attatattta tttccagcat ttaaaaacat ggaaatattt ggcttttatg
      241 atacggcatt tcacattaat cgtgcgttaa gtttagaatc tatttttagc tcccctatta
      301 attttgaaac gtttagatca tatggcatgc aagtaaataa tttttaccca tggttaactc
      361 tatatccttt atttttattg atcaagttta ctaatttagc aataggctat aacctttttc
      421 tttatattgt tacgctcatt actctattta tatgtcatta tgtaatgtat gaaattacca
      481 aaaaacatgt tactagctcc tttttcgcta ttatatatac aacatcttct tttaggagtg
      541 tagaaatatt tcttcgaggg gcaatggggg aattactcgc aatgtcaata cttcctctta
      601 ttttactcgg gtttataaag ctttacgact caaaaaaaga atcttgggtt atgttggcga
      661 ttagcatgac tttattaatt tacactcatg ttctttctgt agctatgact atggtaatga
      721 ttataattgc tttaattatt caattttatc ggaaaaagaa aatagaaatc ttcttaatta
      781 ttaaattaat aaaagctgct atagttactt tacttctttc actagcgttt ataggtccta
      841 tgattgagca gacattgtat caagatctga atagacctta tgtggatata ttacaaaaaa
      901 gagcaattta tctgggtaaa gagtttttga ttggaagtat agattcagaa ttactatcat
      961 ttggtattgg cttaactttg
//
LOCUS       DAYYCW010000242          973 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000242 DAYYCW010000000
VERSION     DAYYCW010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.252"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..586
                     /locus_tag="ACHAYA_003019"
     CDS             <1..586
                     /locus_tag="ACHAYA_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8606732.1"
                     /translation="IYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTD
                     YRNNYSAFKSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYIL
                     RYDDKIIEIHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMY
                     EKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            587..>973
                     /locus_tag="ACHAYA_003020"
     CDS             587..>973
                     /locus_tag="ACHAYA_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8606733.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDE"
BASE COUNT          368 a          105 c          177 g          323 t
ORIGIN      
        1 tatttatgtt gctgctccac tgaggtataa aaatatcatg gaagttgttc gtaatagtat
       61 tcaaagtaaa aaagtatatt ctgtaagagc tatttgttct acttatcttc ctgattggag
      121 aaaaggtact gattatagaa ataattattc tgcctttaaa tctatggggg gaggagtaga
      181 attagactgt atacatgaat tggattatat tataaaccta tttggtttcc ctaaatcagt
      241 aaaaaaacag tttggtaaat attcgaatct tgaaattaac agtaatgatt tagcaacata
      301 tatattgaga tacgatgata aaattataga aattcattta gattattttg gaagagttcc
      361 tacaagaaaa tttcaattgt ttactttaga tgaatgtatc aatgttgact tattaaataa
      421 taaaataagt tttgaagaaa acattgttaa agaaatgcct gaatctgcta atgatatgta
      481 tgagaaggag ttgaaatatt tctttgaaaa cgtagtaaca ggcaaagaaa actggaatga
      541 tttatatcat gcaaacgaag tattgaaaat agctgaggag aaataaatgg aaattttatt
      601 tactatttgt ggtagagctg ggtctaaggg gtttaaaaat aaaaatttga aatatttggc
      661 aggaaagccc ctagtccatt tcgctttagc aatgatagat atctatcaac aaaaacatcc
      721 tgaacataat tgcacgactt gtttgaatac ggacagtaat gagttagtag aattgacgac
      781 taagttaaat ccgtctgtga aaattgtaga acgtaaacct gaattagcta cagatactgt
      841 agggaaaatt gaagtgatac gtgattcatt cttacaacta gaaagagaag ataatatatt
      901 tgatttaatt atagatttag atttgacttc acctttacgt gaagtttcta atcttgaaga
      961 attaatagat gaa
//
LOCUS       DAYYCW010000243          966 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000243 DAYYCW010000000
VERSION     DAYYCW010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.253"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..>966
                     /gene="bsh"
                     /locus_tag="ACHAYA_003021"
     CDS             6..>966
                     /gene="bsh"
                     /locus_tag="ACHAYA_003021"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8606734.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQI"
BASE COUNT          341 a          129 c          170 g          326 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctattactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctggactaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaatta
//
LOCUS       DAYYCW010000244          943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000244 DAYYCW010000000
VERSION     DAYYCW010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.254"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACHAYA_003022"
     rRNA            <1..>943
                     /locus_tag="ACHAYA_003022"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAYYCW010000245          881 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000245 DAYYCW010000000
VERSION     DAYYCW010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.255"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(68..140)
                     /locus_tag="ACHAYA_003023"
     tRNA            complement(68..140)
                     /locus_tag="ACHAYA_003023"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(105..107),aa:His,seq:gtg)
     gene            complement(143..216)
                     /locus_tag="ACHAYA_003024"
     tRNA            complement(143..216)
                     /locus_tag="ACHAYA_003024"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(182..184),aa:Trp,seq:cca)
     gene            complement(223..303)
                     /locus_tag="ACHAYA_003025"
     tRNA            complement(223..303)
                     /locus_tag="ACHAYA_003025"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(267..269),aa:Tyr,seq:gta)
     gene            complement(305..380)
                     /locus_tag="ACHAYA_003026"
     tRNA            complement(305..380)
                     /locus_tag="ACHAYA_003026"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(345..347),aa:Phe,seq:gaa)
     gene            complement(416..488)
                     /locus_tag="ACHAYA_003027"
     tRNA            complement(416..488)
                     /locus_tag="ACHAYA_003027"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(453..455),aa:Asp,seq:gtc)
     gene            complement(498..570)
                     /locus_tag="ACHAYA_003028"
     tRNA            complement(498..570)
                     /locus_tag="ACHAYA_003028"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(535..537),aa:Val,seq:tac)
     gene            complement(586..657)
                     /locus_tag="ACHAYA_003029"
     tRNA            complement(586..657)
                     /locus_tag="ACHAYA_003029"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(622..624),aa:Glu,seq:ttc)
     gene            complement(672..761)
                     /locus_tag="ACHAYA_003030"
     tRNA            complement(672..761)
                     /locus_tag="ACHAYA_003030"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(725..727),aa:Ser,seq:gga)
     gene            complement(801..874)
                     /locus_tag="ACHAYA_003031"
     tRNA            complement(801..874)
                     /locus_tag="ACHAYA_003031"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(838..840),aa:Asn,seq:gtt)
BASE COUNT          247 a          243 c          201 g          190 t
ORIGIN      
        1 cgaaccaacg catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa
       61 taaaaagggc gaatgatggg aatcgaaccc acgaatgccg gagccacaat ccggtgcgtt
      121 aaccacttcg ccacattcgc catggcaggg gcagcaggaa ttgaacccac actaacggtt
      181 ttggagaccg ctgttctacc tttaaactat gcccctataa aatggaggag agtggattcg
      241 aaccactgaa ccctaaggaa cggatttaca gtccgtcgcg tttagccact tcgctactcc
      301 tccatggtgg cgcgggacag aatcgaactg ccgacacacg gagcttcaat ccgttgctct
      361 accaactgag ctaccgcgcc atattttaaa attattaatt gaataacaag aacgacggtt
      421 ccgacgggaa tcgaacccgc gatctcctgc gtgacaggca ggcatgttaa cccctacacc
      481 acggaaccag ttcattatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg
      541 cagatgctct cccagctgag ctaaacctcc aacattcaat taaaatgacc cgtacgggat
      601 tcgaacccgt gttaccgccg tgaaagggcg gtgtcttaac cgcttgacca acgggccagg
      661 taaatttgct acggagagta agggattcga acccttgaga cagtttggca ccatctacat
      721 gatttccaat catgctcctt cggcctctcg gacaactctc cagagtgaaa gaagctcaat
      781 actctttctc aaaatgagaa ctccggcagt aggactcgaa cctacgacat catgattaac
      841 agtcatgcgc tactaccaac tgagctatgc cggaataaca t
//
LOCUS       DAYYCW010000246          873 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000246 DAYYCW010000000
VERSION     DAYYCW010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.256"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..469)
                     /locus_tag="ACHAYA_003032"
     CDS             complement(<1..469)
                     /locus_tag="ACHAYA_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606735.1"
                     /translation="MDKPFLDSVREYLSNPKSTLFPHEDYKIIMNYYLPIGAPQNSKI
                     KIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGL
                     DLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVIT"
BASE COUNT          272 a          155 c          126 g          320 t
ORIGIN      
        1 tagttattac ctgccaccta atcagcaaat ctgaacctga gtcaatataa ttaatattat
       61 tttccatctc caacattgta agtactgttg gtgttaatat agggactttg tacaattgta
      121 tttttttgat tttatcatct gcacaaagga gattttttaa atctaatcct tcttttattt
      181 caaaagttga attttttaaa tctataagag taaaaccaat tccttcatac tgaatttctg
      241 aaaatcgaat ttttctccca gttgatagat caaggggcag aaaacctttt attacattgt
      301 gagtgtcacc atcatttaca atgtcattag gaattttgat tttagaattc tgtggagcac
      361 ctattggaag ataataattc attataattt tgtagtcttc atgaggaaat aaagtacttt
      421 taggattgct aagatattct cgtaccgaat ctaagaatgg cttatccatt gaatcttcat
      481 ccccatttaa actaaaaaac attcctacaa ttgcttttgc aactctaagt gaattcattt
      541 tctttattga atgtgttaaa tcagacttac cattctttaa acttgtattg ttataaagga
      601 aatttttatt gtcttctgca aatctaatat attcctcaac atactcacta ccaaaaaaac
      661 tattacattt actacataac gtattggaat ggcaacagtt tttttgacaa attttataag
      721 gtgcagaact tctttccgta tgctattccg attgtccttg acaatgagcc tcctcggatg
      781 tgacgatctt cggcaggagc taacagttaa agttagactg cctcgctagc gttagcacat
      841 ccgagctcat tatcaaggat tcgctgcgcc aat
//
LOCUS       DAYYCW010000247          821 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000247 DAYYCW010000000
VERSION     DAYYCW010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.258"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACHAYA_003033"
     CDS             <1..156
                     /locus_tag="ACHAYA_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM8606736.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..>821
                     /locus_tag="ACHAYA_003034"
     CDS             175..>821
                     /locus_tag="ACHAYA_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8606737.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFV"
BASE COUNT          361 a           63 c          143 g          254 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg t
//
LOCUS       DAYYCW010000248          813 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000248 DAYYCW010000000
VERSION     DAYYCW010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.259"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            93..785
                     /locus_tag="ACHAYA_003035"
     CDS             93..785
                     /locus_tag="ACHAYA_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8606738.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          358 a          128 c          143 g          184 t
ORIGIN      
        1 aaaattacat atgtaattta aaaacgcttg acattgtgtt tgagatcact tacttttagc
       61 atgtaaaagt attggattaa ggagctatgc aaatgaaaaa gaaatttatt actggtggga
      121 ttgtcgttgc atttctatgt ctaattggct acacaggcac gcggtacgca aatgaaatta
      181 ttttgtgggg tggagagaca gatattgata caatcaatga gaatctgaca cagcttgatc
      241 atacattgac agataaagag caagcgatta cagatcttac cgctcgacta gcacaaaaag
      301 aacagacatt gcaagattca aaggcagatg tccaagaata ccaaacaaaa gtaaatgaat
      361 tagaaaacca aaaaaatcaa tcggcagcag aaaaacaaaa cttagaaagt caattagtca
      421 gcaaggatgc acaactacaa agcaaacaaa atgaaatcaa tgtcaaacta gatgagatca
      481 accaaaaaaa tgatgaaatc aatcatctga atgcagattg gacagcaaga ctacaagaag
      541 cacaagcaaa actttcagaa ttacaaaata ctgtaaatgg tttgaatcaa caaatcgcaa
      601 cactgacaca agaaaaaaca aatgtagaaa ctcagttaac agatacacaa aagaaattag
      661 acgaagcaac tgctgaaaac tcaagctgga aacaatatat tcaaaagtta gaaaaagcaa
      721 aagatgaagt ggaagataca gctgataaat ctcagcaaat tgttgaagaa catttgcgta
      781 aataatcttt cttaacaagt aaacctaaat tag
//
LOCUS       DAYYCW010000249          805 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000249 DAYYCW010000000
VERSION     DAYYCW010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.260"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>805
                     /locus_tag="ACHAYA_003036"
     CDS             <1..>805
                     /locus_tag="ACHAYA_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFM8606739.1"
                     /translation="EILINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGFN
                     SGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQKG
                     ITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIPL
                     KRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVVT
                     DGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV"
BASE COUNT          274 a          117 c          149 g          265 t
ORIGIN      
        1 tggaaatact aatcaataaa ggtgatttgg atgatttgtt tcgtcgtcac ctagaattgg
       61 ctattaacgc tttattacaa gctgaattaa cggtatttct tgactatgaa aagtatgata
      121 gagctggatt taattcaggt aattcccgta atgggaatta ttcgcgctct tttaaaacag
      181 agtacggtga attgaattta gtaataccta gagataggaa tggagacttt tctcaacaaa
      241 ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt cagctagttc
      301 aaaaaggaat tactatgtcc gaaatctctg atctaatcga aaaaatgtat ggtcattatt
      361 acaaaccaca aactatttca aatatgagta aaattgtgtc tgaagatgtt tcggcattta
      421 aagaaagaac tttagaagct aaatactcag tcatttttat ggatgctact catattccct
      481 taaaaagaca aaccgtatca aaagaagctg tctatatagt aattggtatc aggttggatg
      541 gaactaaaga ggttctagga tttactatag ctccaactga atctgcttat gtttggaaag
      601 agattcttca agatctaaaa gaccgtggtt tagaagaggt tttgttagtc gtaacagatg
      661 gtttaagtgg tattgacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat
      721 gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa
      781 tctgtagcga tttcaaatta gttta
//
LOCUS       DAYYCW010000250          802 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000250 DAYYCW010000000
VERSION     DAYYCW010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.261"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..613)
                     /locus_tag="ACHAYA_003037"
     CDS             complement(<1..613)
                     /locus_tag="ACHAYA_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFM8606740.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          240 a          156 c          147 g          259 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tgactggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttccatga gaattgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga
      361 gaaaacgggt acgttttaat tctaaaatag ttaacttttc tttgactttc aatatctgag
      421 tgcgtgtcgt ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg
      481 aaggagagtc atccaatttt cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc cattgagaga ctcctttggt aggatgacgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccac caacacaaaa tattatagaa gcaaaatcaa ttctacctta gatgttctta
      781 tatcactcta tcagtcctat tt
//
LOCUS       DAYYCW010000251          754 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000251 DAYYCW010000000
VERSION     DAYYCW010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.262"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..545
                     /locus_tag="ACHAYA_003038"
     CDS             <1..545
                     /locus_tag="ACHAYA_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8606741.1"
                     /translation="STDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKV
                     MTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSII
                     SSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQSYLILISIIVLSV
                     VFASSLMLIKKPKEILSKIS"
     gene            555..>754
                     /locus_tag="ACHAYA_003039"
     CDS             555..>754
                     /locus_tag="ACHAYA_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8606742.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKN"
BASE COUNT          289 a           75 c          127 g          263 t
ORIGIN      
        1 aatctacaga cttagcctta aacaaattga aagagcttaa aattgatgag tcaaagtatt
       61 ttgttgaaaa agattctaat gcgtttgaag agtctttaga gtcagtgagt ggaataaaat
      121 atataataaa agtaatgact tattctatta tgttaggtgg gatggttgtt ctttcattaa
      181 tcttgattct atggttaagg gaaagaattt atgaaatagg tatattctta tctattggaa
      241 catctaagat acaaattata atgcaattta tattcgagtt aatattcata tcgataccaa
      301 gtataatatc ttccttattt ttaggaaatg tattactaaa agtaattgta gatggattca
      361 ttaactcaga ggattcaatg atttccggtg gaagtttaat aaataatagt agttttatgt
      421 caaatataac aacacttgga caaagttatt taatattaat aagtattatt gttttatcag
      481 ttgtatttgc ttcttcatta atgttaatca agaagcctaa agaaatctta tcaaaaataa
      541 gttaggagaa aataatggat atattagaaa taaagaatgt agcatatagt tatgcaaatt
      601 ctaaagaaaa agttttgtca ggagtaaatc aaaaatttga acttgggaag ttttatgcga
      661 tagtaggaaa gtcaggaaca ggaaaatcta cacttctttc cttacttgct ggacttgata
      721 aacctcaaac aggaaaaata ttgtttaaga atga
//
LOCUS       DAYYCW010000252          714 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000252 DAYYCW010000000
VERSION     DAYYCW010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.263"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..676
                     /locus_tag="ACHAYA_003040"
     CDS             80..676
                     /locus_tag="ACHAYA_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8606743.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          200 a          114 c          137 g          263 t
ORIGIN      
        1 tcatatcaaa aaatggcgca gttagttgct tttctagtaa agtatagtta acaaagaacc
       61 tctaaggagt tgatgaataa tgaatattgc tgcctctcga aaactgataa atttcatatc
      121 cattatcgga ttaggcctat caatcgcgct gacgatttat tttattaatt taggtgtttt
      181 caaagatttg aatgcgttaa gaggattagt aggtgattcc atcattttag gaccgattat
      241 ttttgtcttc atccaaatcc tgcaagtagt tattccaatc attcctggag ggatcagtac
      301 tgcagctggt gtcctgatat ttggccctta tgctggattt atctataact atattggaat
      361 ctgcattggt tcgattatca tttttctgct agggcgtcgt tatggaaagc cattcatact
      421 ttccatgatt agcgataaga catataacaa atatattggc tggctggata atcaaaaccg
      481 ttttgagaag ctgtttgctc tagctatttt tcttccagtt gctcctgacg atgcgttatg
      541 tttgatggct ggattgacaa acatgtcagt gaaacgatat acactaatca ttttgattgc
      601 taaaccactg tcgattttct tatatagtat ggccttgatt tatggaggtc aatatctaag
      661 tggtcttctt ggataatatt tattttgtaa aattagtcat tgattgacaa tgtt
//
LOCUS       DAYYCW010000253          696 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000253 DAYYCW010000000
VERSION     DAYYCW010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.264"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..629)
                     /locus_tag="ACHAYA_003041"
     CDS             complement(54..629)
                     /locus_tag="ACHAYA_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFM8606744.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DAYYCW010000254          691 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000254 DAYYCW010000000
VERSION     DAYYCW010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.265"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..>691)
                     /locus_tag="ACHAYA_003042"
     CDS             complement(14..>691)
                     /locus_tag="ACHAYA_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606745.1"
                     /translation="NYKNLNDLSRSISEYLKGNSYLKIRFSHILILLPYLNENFTLIQ
                     VDKSFIASGNQGNMNITSNKTSRVLLALMGYGILYRDDQNGQILINRAGRSLLMKYRK
                     FIPEDFQNLQLENMRLEDLFRLAEDDKYRFEVLKIQKNELLNKLYPENAIKAHFGDAN
                     HMYSWFIPKRFRGKINAGDQVKVLNRNSTDVVMITDIYVEDEDIIKYMKPVIKLVNKN
                     KGTRGAS"
BASE COUNT          217 a          129 c           81 g          264 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg ttcctttatt tttattaaca agttttatca
       61 caggcttcat atatttgatt atatcttcat cttctacata gatatctgtt atcattacga
      121 catctgtact atttcgattg agtactttca cttgatctcc agcattgatt ttccctctaa
      181 atcttttagg aatgaaccaa ctatacatat gattagcatc accaaaatgt gcttttatcg
      241 cattctcagg atataactta ttcaatagtt cattcttttg aattttcaaa acttcaaatc
      301 tatacttatc atcttctgct aatctaaata aatcttctaa acgcatgttt tctaactgta
      361 agttttgaaa atcttccggt ataaattttc tatatttcat taataatgaa cgcccagccc
      421 gatttattag aatctggcca ttttggtcat ctctataaag aattccgtaa cccatcaacg
      481 ccaatagcac acgtgacgtt ttgttacttg taatattcat atttccttga ttacctgagg
      541 caatgaaaga cttatctact tgaattaggg tgaaattctc attcaaatat ggtagtaaaa
      601 ttaatatatg tgaaaatcga atcttgagat agctattccc cttgagatat tcactgatac
      661 ttctactcaa atcatttaaa tttttataat t
//
LOCUS       DAYYCW010000255          594 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000255 DAYYCW010000000
VERSION     DAYYCW010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.266"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..>594)
                     /locus_tag="ACHAYA_003043"
     CDS             complement(27..>594)
                     /locus_tag="ACHAYA_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8606746.1"
                     /translation="ISYQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPWI
                     IDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTII
                     GEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKEFFT
                     LLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT          207 a          107 c           68 g          212 t
ORIGIN      
        1 taacccaaaa aaaattactt tcatcattaa tttacctcaa tgtcgttaat taagttaata
       61 atatacttat ctttttcaaa agtatattgg ggttccattt tcttttccaa aagcgtgaaa
      121 aattctttaa tctcctctaa atatgcatct tcaataatag tatgtgaata attattatca
      181 tgcataacct catcgtacaa ttgaatatct gtcatactag aagtttcttc attccatgaa
      241 aatagagaat ccgggttacc ttcccattct attaaaatct tttcaccaat tatagttaaa
      301 tttcttttag ctactcttga aacaacatct acacttaaag ttccaactat accagtttca
      361 tgctcaataa taagagtgaa actgtccgaa taatcaatat caagattgga taatttctgg
      421 gtcattacag taaacgattt aactttccca aatgtatcta taatccaagg taattcaata
      481 gcgaatattt ctctacaccc attagttttt ttgtttgaaa caaaaaaatt gcgataatct
      541 tcccaaggat gccagtcggg taaatactgt cctacatggt attgataaga aatt
//
LOCUS       DAYYCW010000256          543 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000256 DAYYCW010000000
VERSION     DAYYCW010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.268"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..492
                     /locus_tag="ACHAYA_003044"
     CDS             22..492
                     /locus_tag="ACHAYA_003044"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM8606747.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          198 a           99 c           81 g          165 t
ORIGIN      
        1 aaatagaaag gagaaaactt tatgtctatt tataattttg aaactacttt agaagacggc
       61 actgtctatt cattgaaaaa atatgcaggc aaaccgataa tcattgttaa cactgcaaca
      121 aaatgcggtc tagcacctca atttaaacaa ttagaagaac tttaccaagc ttatcagaca
      181 caaggtctgg ttatcttagg ctttccttcc aatcaattta aacaagagct agattcagcg
      241 actgaagcag cagaagcttg taaaaccact tatggcgtta ccttccctat gcatcaattg
      301 attgacgtaa atggagaaaa tacagatcct ctttttactt atcttaaaaa tgaagctcct
      361 ggtactgtca ctaaagcaat aaaatggaat tttactaaat ttttgattga tagaaatggc
      421 aaagtaatcg aacgatttgc accacaaact tctccattga aaatgaccgc tgcaattgaa
      481 tccgttcttt aacttaataa aaataaaaaa agatgaacta tttctagttc atctttttgc
      541 ctt
//
LOCUS       DAYYCW010000257          526 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000257 DAYYCW010000000
VERSION     DAYYCW010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.269"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..340)
                     /locus_tag="ACHAYA_003045"
     CDS             complement(<1..340)
                     /locus_tag="ACHAYA_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFM8606748.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVE"
BASE COUNT          151 a          100 c           76 g          199 t
ORIGIN      
        1 tttctactga gatatatttt tcctgaaaga aagccaatac cattttcata gataaagaat
       61 ccgttatata acgagataac agagctttgc tagaacattt tccattaatt tccagacata
      121 tatccaattc actgacggtt actcgcaaat ttttctctag atcgaacttt tgtaaatcat
      181 gtttcagctt atcgatcgtt tttttcaaga gaaatgtcga tagcgacaac tcttcttgaa
      241 cattcgatat tgactctgat aaatttgagc ttgcttctaa atatttaagt aatgaataac
      301 ttctgtgatc ttctttgctt aaaaaaaact cataattcat tagtcatcag ctctttctac
      361 gtatattact ttccatgcat tcaccaacta gctggggata aaactgattt cctggatccg
      421 agtcactgtt gtcagcgatt cttgtttgta taatatacat cgtcctgtct tttttttaac
      481 attttggaga gcgttttatt tggcctttct cgcgatcaaa cctgtt
//
LOCUS       DAYYCW010000258          501 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000258 DAYYCW010000000
VERSION     DAYYCW010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.270"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHAYA_003046"
     CDS             complement(<1..132)
                     /locus_tag="ACHAYA_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8606749.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          179 a           92 c           91 g          139 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa a
//
LOCUS       DAYYCW010000259          500 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000259 DAYYCW010000000
VERSION     DAYYCW010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.271"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..450)
                     /locus_tag="ACHAYA_003047"
     CDS             complement(<1..450)
                     /locus_tag="ACHAYA_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8606750.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
BASE COUNT          144 a           94 c           62 g          200 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaaa
//
LOCUS       DAYYCW010000260          487 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000260 DAYYCW010000000
VERSION     DAYYCW010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.272"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>487)
                     /locus_tag="ACHAYA_003048"
     CDS             complement(<1..>487)
                     /locus_tag="ACHAYA_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8606751.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DAYYCW010000261          486 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000261 DAYYCW010000000
VERSION     DAYYCW010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.273"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..386
                     /locus_tag="ACHAYA_003049"
     CDS             <1..386
                     /locus_tag="ACHAYA_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606752.1"
                     /translation="EEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIVDYFA
                     NTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKEYN
                     YIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT          216 a           47 c           80 g          143 t
ORIGIN      
        1 tggaagaaat agaatggaat aatctgactc aaaataataa aaataattct ggatgtggaa
       61 ttatctttat gtttgttata gttttgggaa tagtagtact tttaataact gtcaagatag
      121 tagattactt tgcgaatact cctgaagaac aaatacagag aaaacttcct gagtttgaaa
      181 gagtaccacc ttatgatcca agagataatt catttcaagt aaaaaaatta aaagagtctc
      241 caaatgaaat gtggtttgaa gttacagttg aatcaagcga cttcaaagaa aaaaagaaag
      301 aatacaatta tattgtaaag gcaaaagtat caaaaagatt tatttttaac cctaaattag
      361 aaatatatag tgttgtaata gaataatagt gttaaaaaaa ttgtaaaagt taaagaagtc
      421 aaattctgat gtgaaaaata aaatatacca tggaagacat aaaagaaaaa ctggataact
      481 agttta
//
LOCUS       DAYYCW010000262          480 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000262 DAYYCW010000000
VERSION     DAYYCW010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.274"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>480
                     /locus_tag="ACHAYA_003050"
     CDS             <1..>480
                     /locus_tag="ACHAYA_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFM8606753.1"
                     /translation="VIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLS
                     SNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTK
                     KSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          158 a           87 c           86 g          149 t
ORIGIN      
        1 cagtgattat tgcttgtgtt atatggggca taattaatgg ctatactagc caaagagcca
       61 cgtatagagc ggtttgttcc gtcttatttc ctaatggtga ctttcctagt aggagtcgat
      121 tcactcgctt aagttcaaac ttagcttaca ctatcaagat tattcgatac tttttcatca
      181 agaagctcac caaaggtgaa ctagtcggaa ttatagatag ttttcctagt ccattatgta
      241 aaccagttag aaatagacaa gcaaaactat tgaatcaaat agccaaagtt ggctataact
      301 caacaaaaaa atcttatttt tatggtctta aaattcatat gatcgttact aagactggct
      361 ttccgattac ttactcaatc acgaatccag gtgtccatga cgtgaaagtg ttagagactt
      421 tatcggaaga agcgaatctt cccaatatcc taggggacaa aggatatatt agccataaaa
//
LOCUS       DAYYCW010000263          455 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000263 DAYYCW010000000
VERSION     DAYYCW010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.275"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>455
                     /locus_tag="ACHAYA_003051"
     CDS             <1..>455
                     /locus_tag="ACHAYA_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8606754.1"
                     /translation="SIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPI
                     TADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTW
                     KEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          184 a           67 c          106 g           98 t
ORIGIN      
        1 agtattgaaa gtggaacaac agtctttgca gtgaaagacg gcgtaagctt accagaagat
       61 aaacttccag tgttgaaagc aaaagatggt tatacagatg cgaaatggcc agaggaagca
      121 actcaaccaa ttacagcaga tgatacagaa tttgtatcaa gtgcaacaaa actggatgat
      181 aaatctgacg ctgacaaata taatcctgaa ggtcaaaaag tgactacaga attgaataag
      241 gaaccagacg catttgaggg aattaagaac aaagaagatt taccaaaaga tactaagtat
      301 acttggaaag aaaaagtaga tgttagcgca gctggaaata aaaaagggac tgttgtagtg
      361 acatatccag atggatcatc tgatgaagtt gaagtagatg ttacagtgac agacaatcgt
      421 tctgacgccg ataaatatga gccaacagta gaagg
//
LOCUS       DAYYCW010000264          451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000264 DAYYCW010000000
VERSION     DAYYCW010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.276"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>451
                     /locus_tag="ACHAYA_003052"
     CDS             <1..>451
                     /locus_tag="ACHAYA_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8606755.1"
                     /translation="FRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVR
                     TLNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNE
                     LLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          147 a           89 c           87 g          128 t
ORIGIN      
        1 tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa atgtatgggc
       61 atcactacac gccccaaacc atgtccaaca tgactaaagt gctgactgaa gaagtaaatg
      121 catttaaagt tagaactcta aatgataagt atgtcgctat tttcatggat gctacttata
      181 ttccattaaa acggcaaacc gtctccaaag aagcaatcta tattgccatt ggtatacgag
      241 aagacggcac taaagaagta ctaagttatg cgattgctcc gactgaatcg acctacgttt
      301 ggaatgagtt gttacaggac atttactcca gaggggttca tgatgtctta ctgtttatta
      361 ccgatggctt aaaaggcatg aaagatacga ttcaccagat ttatcctaaa gctaaatacc
      421 aacattgctg tgttcacgtt tcccgtaata t
//
LOCUS       DAYYCW010000265          369 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000265 DAYYCW010000000
VERSION     DAYYCW010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.278"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..264
                     /locus_tag="ACHAYA_003053"
     CDS             70..264
                     /locus_tag="ACHAYA_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606756.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          165 a           49 c           66 g           89 t
ORIGIN      
        1 gatacataat cattttcact tacatatatt tgaatattaa ctaaaaacag caagaaagaa
       61 gaggatcaaa tgtattatgt agaagtaaaa acaaaaggcg tcaaaaacaa acaatatgtg
      121 aaaggtatca gtaacgaata tcctttactt ggttcatgga aagaagctgc tcctttttcc
      181 aaaccatgtg ccataaaaat aaagaacgag ctagaaaaag aactgacttg tggtaaagct
      241 gttgtagata tcatcgaaaa gtaaatccaa tgtatagaaa ggaagatcac acatgaaaag
      301 cgacgtacaa attgctaaga ttatagaatt agaagaagaa gacgcagtgt tgacaattga
      361 aaaaaaata
//
LOCUS       DAYYCW010000266          345 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000266 DAYYCW010000000
VERSION     DAYYCW010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.283"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>345
                     /locus_tag="ACHAYA_003054"
     CDS             <1..>345
                     /locus_tag="ACHAYA_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8606757.1"
                     /translation="AEEFIRSRKAYGSRKIKKALSKRGIQISRRKISRIMKNRGLKSS
                     YTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLF
                     NREILGYSCGEH"
BASE COUNT          129 a           60 c           66 g           90 t
ORIGIN      
        1 cgctgaagaa tttatccgca gccgaaaggc ctacggctca agaaaaataa aaaaagcctt
       61 atcaaaacga ggcattcaga tcagccgacg aaaaattagt agaatcatga aaaatagagg
      121 attaaaatcg agctatactg ttgcttattt taaagtacat cattctactt gcaatgaagc
      181 caaaacgaca aacgtattga atcgtaaatt cttaagagac aacccattag aagcgatcgt
      241 aacagacttg acttatgtac gagtcgggaa aaaatggaat tatgtctgtt tcattttgga
      301 tctgttcaat cgagaaattc tcggctattc ttgtggagaa cataa
//
LOCUS       DAYYCW010000267          309 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000267 DAYYCW010000000
VERSION     DAYYCW010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.284"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..309
                     /locus_tag="ACHAYA_003055"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACHAYA_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAYYCW010000268          308 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000268 DAYYCW010000000
VERSION     DAYYCW010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.285"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>308
                     /locus_tag="ACHAYA_003056"
     CDS             <1..>308
                     /locus_tag="ACHAYA_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8606758.1"
                     /translation="MRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTE
                     KVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSF
                     "
BASE COUNT           98 a           59 c           54 g           97 t
ORIGIN      
        1 ttatgcgctt aaaaaagcag cgctatacct gtaaaaactg tcgaacccat tggacggctc
       61 aaagttattt tgttcaaccc agacattcaa tcgcgaatca tgtcagatat aaaattgctt
      121 ctttactgac tgaaaaagta tctttatctt ttattgcgaa aaattgtcag gtatctttga
      181 ccaccgttat tcgtacgttg aaagagttta aaagctactt accaaagcaa tctaagagga
      241 ttctccccag agtattgatg gttgatgaat ttcgttcgca cgcttccata gaagataaaa
      301 tgagcttt
//
LOCUS       DAYYCW010000269          282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000269 DAYYCW010000000
VERSION     DAYYCW010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.287"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACHAYA_003057"
     CDS             <1..>282
                     /locus_tag="ACHAYA_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM8606759.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAYYCW010000270          265 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000270 DAYYCW010000000
VERSION     DAYYCW010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.denovo.288"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>265)
                     /locus_tag="ACHAYA_003058"
     rRNA            complement(<1..>265)
                     /locus_tag="ACHAYA_003058"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           56 a           89 c           51 g           69 t
ORIGIN      
        1 actcctcttc agacttactg cttggacaga catttccgat cgtctgcatt ccttagcctc
       61 ctgcgtccct ccattgctca aacaaaaaca acgggtacag gaatatcaac ctgttatcca
      121 tcgcctacgc ctgtcggcct cggcttaggt cccgactaac cctgggcgga cgagccttcc
      181 ccaggaaacc ttagtcattc ggtggacagg attctcacct gtctttcgct actcataccg
      241 gcattctcac ttctaagcgc tccag
//
LOCUS       DAYYCW010000271        17094 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.guided.061, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000271 DAYYCW010000000
VERSION     DAYYCW010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.guided.061"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..109
                     /locus_tag="ACHAYA_003059"
     CDS             <1..109
                     /locus_tag="ACHAYA_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HFM8606760.1"
                     /translation="YVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            132..383
                     /locus_tag="ACHAYA_003060"
     CDS             132..383
                     /locus_tag="ACHAYA_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM8606761.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            373..1602
                     /locus_tag="ACHAYA_003061"
     CDS             373..1602
                     /locus_tag="ACHAYA_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM8606762.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            1599..3680
                     /locus_tag="ACHAYA_003062"
     CDS             1599..3680
                     /locus_tag="ACHAYA_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM8606763.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            3829..7749
                     /locus_tag="ACHAYA_003063"
     CDS             3829..7749
                     /locus_tag="ACHAYA_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM8606764.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            7750..8694
                     /locus_tag="ACHAYA_003064"
     CDS             7750..8694
                     /locus_tag="ACHAYA_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606765.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            8698..8901
                     /locus_tag="ACHAYA_003065"
     CDS             8698..8901
                     /locus_tag="ACHAYA_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM8606766.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(9201..9437)
                     /locus_tag="ACHAYA_003066"
     CDS             complement(9201..9437)
                     /locus_tag="ACHAYA_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8606767.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(9620..9991)
                     /locus_tag="ACHAYA_003067"
     CDS             complement(9620..9991)
                     /locus_tag="ACHAYA_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM8606768.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(9988..11316)
                     /locus_tag="ACHAYA_003068"
     CDS             complement(9988..11316)
                     /locus_tag="ACHAYA_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8606769.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(11277..11606)
                     /gene="mobC"
                     /locus_tag="ACHAYA_003069"
     CDS             complement(11277..11606)
                     /gene="mobC"
                     /locus_tag="ACHAYA_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8606770.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(11864..12234)
                     /locus_tag="ACHAYA_003070"
                     /pseudo
     CDS             complement(11864..12234)
                     /locus_tag="ACHAYA_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            12379..12564
                     /locus_tag="ACHAYA_003071"
     CDS             12379..12564
                     /locus_tag="ACHAYA_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8606771.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            12722..12889
                     /locus_tag="ACHAYA_003072"
     CDS             12722..12889
                     /locus_tag="ACHAYA_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606772.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            13507..14166
                     /gene="vanR-B"
                     /locus_tag="ACHAYA_003073"
     CDS             13507..14166
                     /gene="vanR-B"
                     /locus_tag="ACHAYA_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8606773.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            14169..15530
                     /gene="vanS-B"
                     /locus_tag="ACHAYA_003074"
     CDS             14169..15530
                     /gene="vanS-B"
                     /locus_tag="ACHAYA_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8606774.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(15621..16922)
                     /locus_tag="ACHAYA_003075"
     CDS             complement(15621..16922)
                     /locus_tag="ACHAYA_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8606775.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4237 a         4380 c         4586 g         3891 t
ORIGIN      
        1 gtatgtgggc aacaatgtga tgctgcattg cgggaacccg atttcctaca cgaaccttac
       61 ttcaaactac tggcaatccc atttttacag ttacgggcgt ttaccttaac gccagaaagg
      121 agctttcaat tatggcaacc aaacttgacc gcatcgaaaa ggacatccag aaaaccaagt
      181 ctaagatcgc ggagtttcag aaacagcttc gggagctgga aacgcagaaa accgaacagg
      241 agaatttgca gatcatccag cttgtgcgcg gcatgaacat gaagccggag gaatttgccg
      301 cgttcttacg cagcggcgca atgcaggccg cccctgccgc aaccccttac catgaacagg
      361 aggacaatgc ccatgaagaa taagaccaga cggatttccc gtattctccc cgcacttctg
      421 gccgtgatgc tctgcgtcac ggcctttccc gttaccgccc ttgcaggcgg caatgacccg
      481 gcaccggccc cgctgccgga ggctacaacc gaacccgcta cgggcggttt ggagcctgaa
      541 acagacgaaa ccggcaccgc cacggacggg acagaaaccg aggccgaaaa agataccggc
      601 cctctgaccg gcaaggacat caccgacctg ttttccgcgc tgttcggttc caaggtgagc
      661 attgccgcca cggacgacgg catccagatc accaccggca ccgacaaaaa agaacctgaa
      721 cagaccggca ccgtcaccac caacggcggc aggctgaatg tccgcaccgg cgcggggctt
      781 gacaagaccg cgttcaccca gcttcccaat gggacgacgg ttgaggtagt cggcacagac
      841 ggcgactgga ttaaaattct cttgccggag cgcatcggct atgtccattc cgactatatg
      901 accgtcagcg aaaaggaagt agcggctacc ggcgagggcg gtttttccct ctccattgac
      961 ccggaggaaa ttgcttctct gctggaactg ttcaatggcg gcggggccag cgccgccctg
     1021 tccccggacg ggaacctgtc actgattgac gacatcggca gcgccgcgaa aaatggcaag
     1081 cagtttatca ccgtggaaac caaaaacggc aatgtgtttt atctgattat tgaccgggac
     1141 gacgagggaa aagaaactgt ccatttctta aatcaggtgg atgaagccga tctgctgacg
     1201 ctgatggagg acggggaaac cgtcaccccc gctgctgtct gctcctgtac cacgaaatgc
     1261 aaggccgggg ctgtgaacac gaattgcccg gtatgtaaaa ccaacctgac cgagtgcagc
     1321 ggcccggagc cgcaggaacc ccagccggag gaacccgaag ccccggagga agaacccaag
     1381 ggcggcgcgg gcggcctgat cgtgttcctg ctggtggcgc tggcgggcgg cggtgcggcc
     1441 ctttactatt tcaagttcaa aaagcccaag gctgaaacca ccggcagcga cgatttgggc
     1501 gagtatgatt ttggggagga cgaggatttg gaggacgagg aacccgaaac cgagtttgac
     1561 ccggaagctg attttgtgag tgaacaggag gacgaggaat gacatataaa cttgtaatcg
     1621 cggaaaaacc cagcgtggcc cgcagcattg cgggcgtgat cggggccacg gagaaacacg
     1681 acggctattt ggaggggaac ggctatctgg tgagctggtg catcggacat ctggtgtcct
     1741 ttgccgacgc cggacgctat gacgagcgtt tcaaaaaatg gcgctatgag gatttgccca
     1801 ttctcccgga aacatggcag tacatcgtcc ccgacgacaa gaaacagcag tttgacgttc
     1861 tctgttcgct gatgcagcgc cccgacgtga ccggccttgt gtgcgccacc gacgccgggc
     1921 gcgaggggga attgattttc cgtttcgtct accagatggc gggatgccaa aagccctttg
     1981 agcgcctttg gatttcctct atggaggaca gtgctattaa ggacggcttt gcccacctga
     2041 aaccgggcgg cgactatgat ccgctgtatc aatcggccct gtgccgggcc aaggccgact
     2101 ggctgatcgg cattaacgcc acccggcttt tttccgtcct gtaccacaag aacctgactg
     2161 tgggccgcgt ccagacgccg accttaaaaa tgctggtgga cagggacgcc aagattaccg
     2221 atttcaagaa agagaaatat cacatcgtcc atatcacggc gggcggcgcg gacgcggtaa
     2281 gcagccgctt ttccgacgct gccgaggcaa acaccgtcaa ggcggcttgt gcaaaagcgc
     2341 aggccgtttg cgtttccgtg acgcgggaga aaaagacgga acagccgccc aagctctatg
     2401 accttaccac cttgcagcgg gaggctaacc ggcttttcgg ctataccgcc aagcagaccc
     2461 ttgactatgc ccaggcactg tatctaaaga agctgctgac ctatccgaga acggatagtc
     2521 aatatcttac cgacgatatg ctgcccgccg tggaaagcct tgtgtccggc ctgtgggagc
     2581 tggtgccgtt tgccaagggc ctgaaccttt ccccgcagtt tgaccgcatc ctgaacagca
     2641 aaaaggtgtc cgaccaccac gccattatcc ccaccgctga atttgtgaag cagggctttg
     2701 gcactctggc cgagagtgaa aaaaagctgc tgtccctgat ctgctgcaag ctcttatgtg
     2761 ccgtggccgg gccgcatatc tatgaggccg tcaccgccac atttacctgt gccgggcagg
     2821 agtttaccgc caagggcaag accgtccttt cccccggctg gaaagagatt gaacgccggt
     2881 tccgttcttc cttaaagacc gacgccgacg aggacgccga ggcggtgcgg gagctgccgg
     2941 agctttgcga gggccagacc tttgcagacg tggccgcctc tgtcaccgag catttcacca
     3001 cgccgcccaa gccctacacc gaagatacgc ttttgtccgc tatggaacgg gccggggccg
     3061 aggatatgcc ggaggatgcc gagcgcaagg gattaggcac accggccacc cgcgccgcca
     3121 ttctggaaaa gctggtgcag atgggctttg tccagagaaa aggcaagcag ctcattccca
     3181 ccaaggacgg catcaatctg gctgtggtgc tgccggaggc gcttacttct ccccagctta
     3241 ctgccgaatg ggaaaaccgg ctgaccgaga tcgccaaggg gcaggccgat cctgacgaat
     3301 ttatggacgg catcgagacg caggcgcggg agctggtgca gacctattcc tgtatcagtg
     3361 aggaaaagca gaaattgttt caggccgagc gtgttcctat cgggacgtgt ccccgctgtg
     3421 gtgaggctgt ctatgagggc aagaaaaact actactgcgg aaaccgggcc tgccagttcg
     3481 tcatgtggaa gaatgaccga ttttttgagg aacgcaagaa agcgttcacc ccgaagatcg
     3541 ccgctgcgct gctcaaaacc ggcaaagtca aggtaaaggg cctctattcc gtaaagaccg
     3601 gcaaaaccta cgacgggacg gtgcttttgg ctgataccgg gggcaagtac gtcaactacc
     3661 gcattgaaca caggagctaa aggacagtaa cacagcaagc atagggagtg tggctacact
     3721 cccggtaaaa tcccccggcc gcgtccctgc cgggccgggg atttttgctt gttggggaag
     3781 tccccaaccc cctatttttt gaaacagaac agagaaagga gtgaccgcgt gaacgaagta
     3841 ttttctatcc aagtccataa ccggcagttt ttggaacagg gcaaacaagg cgtctggctg
     3901 gatttgccca ccacaaccga aacgctgcaa gccgccctgc gggatgttgg cattactgcc
     3961 gacaatccgc aggatttttt tatcaacggg tattcctgcc cggaggacag acaccttgcc
     4021 attccctatg acatggtgct ggccgccgac gtggacgagc tgaatttcct tgccgcccgg
     4081 ctgggccagc ttgacgccgc cgagatcgca gagctgaacg ccgccctgca aaatccccgt
     4141 ggcggctttg agaatatcgg ccagatcatc gactacaccg aaaatgtgga ttactatgtg
     4201 catcttcccg acgttagggg ggatggacag ttgggcgact actacctgaa ccgttccggc
     4261 atggtggata tgccggagga atggaaagcc gggattgata cggcgcagtt tggacgccac
     4321 attgcccagc aggaacaagg cgtttttacc cagtacggct atctggtgag aagcggcgac
     4381 gaatggcagc gcgtccatga gggccagccg gtgccggagg aataccgggt gatgcactac
     4441 ccccagccgg aagctgaccg ggaggaaccg caggcccagc agccggaggc atccgaaagc
     4501 cccgacaaag cgccgccccc ggttatcccg atcatgctgg acggcatgaa cagcaaagaa
     4561 cgcatgaaag tgattactga ccgtctggaa accggcgtac aggagctttt tgaaagtagc
     4621 cgctatatgg cttacttatc cgctatgtcc aagatgcacg attacagtct gaacaatacg
     4681 ctgctgatcg ccatgcaggg cggttcgatt gtggcgggct ataacaggtg gcaggatgaa
     4741 tttgaccgcc atgtgaagcg gggcgagaaa ggcattaaga tactggcccc ggccccgttc
     4801 aaggttaagc agcagatgga gaaaaccgac ccggccaccc agcagcccgt gatcggcgcg
     4861 gacggcaagc ccgtgaccga agaacaggaa gtgacggttc cggcctttaa ggtggtgaca
     4921 gtctttgaca tcagccagac cgagggcaag gattttccca tgccctatat cagtgaactt
     4981 accggcgacg tgcagcagta tcaggatttc tttgccgcgc tggaacggac atcaccctat
     5041 cccataagca ttgagccgat ttcagacggg gccaagggcc gctgcttcta cgcaaaacag
     5101 cgcattgcca tcaacgaggg catgagcgaa ttgcagaccc taaagaccgc catccatgag
     5161 attgcccatg ccaagctcca cgccattgac cgggaggccc ccgccgagga acaggcggcc
     5221 cggccagacc gcagcacccg cgaggtacag gccgaaagcg tggcctatac cgtctgccag
     5281 tattacgggc tggacacgtc ggactattct tttggctatg tggccgattg gagcagcggc
     5341 agggagttat ccgagctgaa agcctccctt gaaaccatcc gggccacggc cagcgaattg
     5401 ataaccgaga tcgacggcca ttttgcccag cttcagcagg agcgcgaggc ccggcaggaa
     5461 caggaggccg tcacggttga cgttaccccg gaggcagcgg ccccggcgca ggccgaggaa
     5521 gaacaggccc cggcccttga tccggcagcc gagccgcttg tgactgtgct atggagcgaa
     5581 agcccccagc taaaggacgg ccagcaaatg ccgttgcatg aggccgacgc gctgtttgcc
     5641 cgtctggatg cagcatatcc ggcagaggca ggctatgaca aaaccgcgtt ccgcattgac
     5701 tgtatctttc agggcgagcc ggacagctac gaggggcggc aggattttgg agatagggac
     5761 ggttccctga tcgaacatat ccagagctac caccaatttt acgcgcagga cgaaaactgg
     5821 aaaaagcatg taatcgctac aaccggcata gagggctggg agcaggacaa ggcccatcgg
     5881 gatatgctgt taggcgagtt tgtcccctat atgaagctgc attgcagcct tgcccaaatg
     5941 gaacagacgg cgcagggccg cttacagtcc ggggaaaccc tgacgccgga ggaaacggcc
     6001 tatttggggg cgcttctctc ttatgtgcag gagtgccgcc ccatgctcaa tcagggccag
     6061 tatcagttgc cggagccgcc gaaactggct gacttcgacc agagcttgca ggactacaaa
     6121 gcacaggtac aggccgagat cgcgcaggag gccgccgcag ccggtatgac cgtggaggaa
     6181 tacgcggcgg ctggctatga agcaccccaa caggacacct tttccatcta ccagcttcga
     6241 ggcggggagg aaacccgcga ctatcggttt gagccatacg agcgtttaca ggccgcaggg
     6301 ctggccgtgg acagtgccaa ctatgacctt gtttatacgg caccgctgga taaaagcacg
     6361 tctttggagg atatttaccg cacattcaac atcgaccacc cggcagattt taggggacac
     6421 agcctttccg tttccgacgt ggtggtgctg caccagaatg gacaggacac cgcccactat
     6481 gtagacagct tcggctatat ggaagtgccg gagtttttgc gggaaaatcc cctgcgtacc
     6541 gccgaactgt ctacggagca gaacgaaaac atgattgacg gcattatcaa caatacgccg
     6601 cccgaaccgc agccggagga acccgaacag ctatcttttg aggatgtggc agttgcttcc
     6661 ggccagacac aggaggaacc cccggaggct gcgcccccgc aaacggctgt ccgctattat
     6721 cccatcaatg agggagccgc ccgccgcgcc aaggaagcca tgagcttttc tgactaccgg
     6781 cccggcagcg ccacggcgga ataccggcac tatgtcgatc aggccgtgga aattgcggag
     6841 cgccagaaaa agcgtgtgga gccagagtat cacgaaaaga tagacagcct ccttgacacc
     6901 tacgcccgca agctggccga gaacatgaat aagggctatg agattaccgc ccgcgtcccc
     6961 tctgtcctga ttgccgggcc gtccaatttc ccggtacggg cgaaagaaaa gcagaacgcc
     7021 gccagtgacc gcaacatgga ggaatttcag tacattcagg gcttgcttga caaaatccgc
     7081 agcaccggca tgggtggtat cagcgccgac gatccgcagg ccgtttccaa gctggaaaag
     7141 aaactggaaa agctggaagc gtcgcaggag ttgatgaagg ccgtcaatgc ctactaccgc
     7201 aagcatggga cgctggacgg atgcccccat ctgatcgaaa gaggcattga aaacctgaaa
     7261 gcggatatgg cgtcgggctg gcactatgag aaaaaaccgt tccagtcctg gcagctctcc
     7321 aacaataacg ccgagatacg ccggttaaag ggccgcattg aggaattgac ccgccaaaag
     7381 gaggcggcct atgtgggatg ggagtttgac ggcggcactg tggagatcaa ccgggtggcc
     7441 aaccgcctgc aaatcttctt tgaggggaag ccggatgctg ctgtacgcga cgagctgaaa
     7501 agcaacggtt tccgctggtc gcccaaggcc ggggcatggc agcggcagtt aaacgacacc
     7561 acaatccgcg tcgctgaccg tatcaagtgc atccagcccc tttccggcga aaagcccagc
     7621 gccttacagc ttgcggcccg cagggagaaa gaaaagccct ctatccgggc gcagctcaac
     7681 gcggccaaga ccgaacaggc caagcagcca ccggcgcggg aaaaatcaaa gggattggag
     7741 gtagagtaaa tgcagaagtt tgaaaatgtg gacgttctgg ccgcgctgga acagatcatg
     7801 cggcagaaca ccgcgtttta tcaaagtgac tttggcattg acaaagacat tatcaggcgg
     7861 gatgccgcca gcgatcaggc cgcagacaag accctgctct ggatgtcccg gccctccggg
     7921 acgtactgtt tccgggaacg tgatgttttc ctgaaagaca cccgacagta caacacatgg
     7981 aaattttacg gggagcagac ccgtgacaag atactggcct acgcggtgga gctgaccggc
     8041 acacagggcg gcactatccg gggcaatctc tatgagctgg actatccgca gcatttccgg
     8101 cacgtcattg aaaccgccca gccggtgagt gtgaaccggc tcttttatga gcatggcacc
     8161 cgtgacatcc cggagagcca gcgttttgac ggaagccctg accgggtatt gggaaacttt
     8221 ttgtactatg aggcccagcc ccatgacccg gctgtgctgc aggaggcgct acggacggag
     8281 cagcacagcc gggggcgggc cgtccccggc gatttcaaag tccacgtcac cgctttgcat
     8341 gacagccgga ttgaaaccga ggcccggcgt attgtggaca gcctgaaaga tctgtccgcg
     8401 cctaacagcc ccaataaaac gcatttcatg gtggagatct ccccgcactt tgcggcgatt
     8461 gcttccacca aggataatga ccggctcttt gccatgctgc cctataaatc cctgtgtttt
     8521 accgggatga aaggcaggca tggcgtgtat gccgtgatta accgggacga gcgccgggat
     8581 gtgagtattc gcaggccccg gccctctatc cgcaagcagc tatccgacag caaacaggcc
     8641 gccagcccaa aaaaggcggc ggccagaacc aaaaagaatg aattggaggt ataaggaatg
     8701 atgcacttta ccgtggagga agaaaacctg atctgtatgt accacaatgc agaccggcgc
     8761 agaacgataa acaggctcac cgccgtcatg ccggttatgg atggggatat gcgggcgcag
     8821 gcccagcaga ccctttccaa gctggaacgc atgacggatg ccgactatga cggccagaag
     8881 ttccacttca cagacgaata acgaacagca gacgccgggc gcggcggcca tgtggtcgct
     8941 tgcgtccggc gtcttttctt tttgcctatt cctacgcatt tagaacccgg tattgtcggg
     9001 gataggatga agtattcgct tgcccctgca aacatgcggg agaagccttg cagcgggcgg
     9061 ttttcaatcc cttaaatgcg taggttgcag ctataattag acccctgaaa cagatatagc
     9121 cgcgcagcgg gtgtatctgt ttcaggggtg tgcaggtgat agccgcaaag cggcgtaaga
     9181 gggtgcagcc ctcttgccgt ctatctctgt tccagcggga agctcggcaa tgcttccagc
     9241 agtttcaggg aaatgcgctg ccgtatatct tcgtcaatga ctttcttctt tttgccgttt
     9301 gcctgtctga tctccaccgt ggaaagctcg ttgatgtagt cagcataatg ggccaacact
     9361 ttttccgtgg cccacctctc accggcaacg gcggctttga tcgtttcata gtccagcagc
     9421 ttttcttgtc cgtccatcct gatacccccg tcagactgat tttttctatg ataccatatt
     9481 cggaagcatg gaaccagaga aaacggcgct tttctgctgg taaattctac gcatttagaa
     9541 cagcacttcg gcggggatag gataaaacac ccacataccc cgctgaccat gccggaaaag
     9601 ccttgaaata taagggcttt tacagctcta agtgcgtagg tttccagtat cttttccgct
     9661 ggcgctcggc ctctttgcgg cggcgcatcc gtttggagca ggccgcgcag tatttggccc
     9721 ggttagactt cggcgtgaac gtggccccgc actcacagca gcgtttcact tcgtcccggt
     9781 ctttggtgat ctcggcatac agggccgcat ccagcgggag gacagcggcc ttaaaccagc
     9841 ggcacagaag cgaataggaa atgctctgta cgcaaataca ttcttcgccg tcgtccagca
     9901 acaggcagtt gccgtcacaa tagttgcagc actcatgcgt cagcttccgg gccttgcgat
     9961 attgagggta attcattcgt ggaatactca tagctcttgc gtccattcct ttcccggcgt
    10021 catacgcaaa atgccgtcca cattctgctt gatgatgccg tattcctgca tctgcttttt
    10081 cgcttcgccg tacttctcat acagccgttc cttttcctcg gccaatcgct tgtactcggc
    10141 tttcagggcg tacagatcgg ggagcttttt cacaccctgt ccctttaggg cctttgccgc
    10201 agcttcatac aaaatcaacg tgctttcatg ctcacgccgg aattttgcct tatccgctgc
    10261 attgcggtat tccatagcca cgggccagag ctgcttgtag gtagaaatat tctttatcag
    10321 caccgccata tccgcaagcc ggtgttccat agctttgagg gtagcggcgg cctcgtcgct
    10381 ggcggcggtg atctcggcca ctttgctttc caaatccggg taggtgtcta tcccatgttc
    10441 agtcaggaaa ttcaaagtct ttgcggcctg cttcaaattg tggagcttcg cccacctctc
    10501 atagcctgcc gactgctggg ccttaatgtt gttttccaga tcaatccgca aagaaatccc
    10561 cttgcgctct ttgggcgctc tggtgataac gcggcctttg atacgctctt tgatggcttc
    10621 ctcggtgtag gcttccccca atgtcttgca gcgggtaaag cgttcctgtc cgggagcgcg
    10681 gaaagaaata aactttcccg gcttgatctc atagccctgc gcctgcatga gccgcaggaa
    10741 atcatcaaaa tcttttgcct gcgggatagc ggcgtcaatg gctgctttga gcttcgcttt
    10801 ccagctcgtc cctttccgct gggcgtccca ctcggcatag ctcttgccct tactctggcc
    10861 gggcttcacc acggacaatc cgttttcctt gcacagcctg tcgctggtgc gccggatata
    10921 ggcatagctc tgtttgttgg aaatgtactt gcgctgattt accatatcca cggcacagaa
    10981 aataatgtga ttgtggacgt ggcccttatc aatatgggta gtcagcacat agggatattt
    11041 cccttgcagc acttcctcgg caagctgctt cccgatctca tgggcctgtt ccggcgtggc
    11101 ttcgccgggg gcaaaggatt gtatcaggtg gtggcccaga ttattgccct tttggtatgc
    11161 ctgcgccagc agcatttcaa attcaatgtc cgctgtttca taagagcagg caaaggaggc
    11221 caccagcagc ttgccgtcag ttttgtccgg gttctcgatg tagtcaaggg cttttttcag
    11281 cgttgacttg ataggatgta tctttgtaac tgccatatct gcgccagcgc cccctttata
    11341 tcctcgatgt cctgtgcgta caccggcccc ataccgttca gcctctttgc aatctgattg
    11401 acgttgacgc cgatcttctg tatctcggcg gtctgcgccc ggatgtcgct ggtgtccatg
    11461 ttgacgatat agccgtcaat gagcatcttc cgggcgtagg ctgaaaagtt cttcgtatgc
    11521 agcagcgcca ttttctgttc aatcatagcc cgttcctccg gcgtcacagg gacgcgcagc
    11581 acgatttttc gctttctgtt tgccatgctt caccgtcctt tccattcagg ggtttgggga
    11641 tttccccaac aagcaaaacg gacgcggccc ggcagggacg gggcaggccg tttttccggg
    11701 agtggctaca ctccctatgc ttgctacgtt actgtttctc cactaacaag acgtttccaa
    11761 agccccaaac tacccgcaaa atagccctct ggaaagaaaa aagtgcaaaa aaatagaagc
    11821 tacgctgtgc agctcccgct aaaagtaaaa cagaggatgc cagttacaca tcctccgttt
    11881 cattcttctt cggcttttca attagtatgg cgctgtcaat cgcgccctcg atgatcggca
    11941 ggtattcttc ggggcagagt ttcagcttgt ggctgaccct ttgccgcagt tcgctttcct
    12001 cccgcatgat ctccgggtta aagtagcgtt ccacaggaag cccgcaggct ttaataagct
    12061 gtatgacaac cggcagactg ggaatggccc gtcgttttcg atgttggcaa gatagcgcca
    12121 gtcaatattt attttctctg ccattgcttt gcgcgaaacg cgctttgctt gccgggccgc
    12181 tttcacatcc gcgccgaaag tttcaaagcc gggacaatct tcaatattcg ccatatcgca
    12241 cctcccactt acattgtata tttcatactc tgcccgtgga atgttggtat atgcaccttt
    12301 cttaaagttt atatttcaca ttttgtttct tttattcttc gagcaaaaga attataatgt
    12361 accctgtgga ggtgtactat ggactacatg acgttgaaag aggcaggcga gaaatggggt
    12421 gtgcccccac gatggataaa ctactattgt gctgcgggac gcatccccgg cgctgtaaag
    12481 atggcgacga tctggctcat tcccaaaact gcggaaaagc cgattgacag gcgtacaaag
    12541 aaaggaaagg cggttactga atagtataat gaaaccggcg cattttggat atggttttga
    12601 agagagagaa agcatggtat tttgtcacgg aaaaactata agatgataga tggcaggctg
    12661 ctccagaccg ataaaaagta ttctcatctg aaacagaaag aaaagattta cacatggatg
    12721 tatgaagaag cgaaacttta tcatgacaga aatggaaaat gcccgaaaca aagacgagga
    12781 agctgctgct gtatcagccg tatatggtag aattgaaaag gccggcatct ggattcttta
    12841 tggtgaagtc tccagacatt ggataactgc tggtctaatg cgcctttgag tatggggtga
    12901 ttgattttaa taccggggag gtacggcttt ttgaggaaaa tattctggga tttacatttg
    12961 gcgattatta catttcctgt aactgcaagg acacggatga tgaagatatg aggaatatag
    13021 acttgctgat tttctactgt cttgaacggt attaggactg aatctgctgc gcggagtgcg
    13081 gcaagagcga ccggagggag tacacacagg ccctgcctgt gtgaaaagcc ataccgcaag
    13141 gtgcattcgc aaaaaagagg ccgcgcattt tgctgaggct tggggtgtcc ccaacaagca
    13201 aaacgagaac gccgcaggcg ggcaagtggt ggatcagaat atccgtaaag gatattctga
    13261 tccattgctt gccagtatta tcgggatgtt ttgacaaccg ctgtaatcga aacatcccaa
    13321 acattaagaa ggtgttaatt attattatct tgatttaatt cgtttaaacc aataatatag
    13381 atacatacac catgaaaaga agcgagtatc ccaatgacgg atgattaaaa tccgaaaaga
    13441 aatcgctttg aaactacagg gaaactacag actgttatgt tatcttctta aaatggaggg
    13501 atttttatgt cgatacgaat tctacttgtc gaggatgatg atcatatctg taatacagta
    13561 agggcgtttc tggctgaggc aggatatcag gtggatgcct gcacagatgg aaatgaggca
    13621 tacaccaagt tttacgaaaa cacttatcaa ctggttattc ttgatattat gctgcccggt
    13681 atgaacgggc atgaactttt gcgtgaattt cgtgcgaaaa atgatactcc cattctgatg
    13741 atgacagccc tgtcggatga cgaaaaccaa atccgggcgt ttgatgcaga ggcagacgac
    13801 tatgtaacaa agccattcaa gatgcagatt ttactaaagc gggtggaagc cctgctgcgg
    13861 cgcagcggtg cgctggcaaa ggaaatccgt gtcggcaggc tgacacttct gccggaggat
    13921 ttcacggtac tttgtgacgg tacagagctg cccctgacac gaaaagaatt tgaaatcctt
    13981 ttgctgctgg tgcagaacaa aggcagaacc ttaacgcatg aaatcatttt gtcacgtata
    14041 tggggatatg actttgaggg tgatggcagc acagtccaca ctcatatcaa aaatttgcgg
    14101 gccaagctgc cggaaaatat catcaaaacc attcgcggtg taggataccg attggaggaa
    14161 tcataataat ggaaagaaaa gggattttca ttaaggtttt ttcctatacg atcattgtcc
    14221 tgctactgct tgtcggtgta acagcaacac tgtttgcaca gcaatttgtg tcttatttca
    14281 gagtgatgga attacagcaa acagtaaaat cctatcagcc attggtggaa ctgattcaga
    14341 atagcgatag gcttgatatt caagaggtgg cagggctgtt tcactacaat aaccaatcct
    14401 ttgagtttta tattgaagat aaagagggaa gcgtactcta tgccacacca aatgccaata
    14461 catcaaatag ttttagaccc aactttcttt atgtggtaca tagagatgat aatatttcga
    14521 ttgttgctca aagcaaggca ggtgtgggat tgctttatca agggctgaca attcggggaa
    14581 ttgttatgat tgcgataatg gttgtattca gccttttatg cgcgtatatc tttgcgcggc
    14641 aaatgacaac gccgatcaaa gccttagcgg acagtgcgaa taaaatggca aacctgaaag
    14701 atgtaccgcc gccgctggag cgaaaggatg agcttggcgc actggctcat gatatgcatt
    14761 ccatgtatgt caggctgaaa gaaaccatcg caaggctgga ggatgaaatc gcaagggaac
    14821 atgagttgga ggaaacacag cgatatttct ttgcggcagc ttctcatgag ctaaaaacgc
    14881 ccatcgcggc tacaagcgtt ctgttggagg gaatgcttga aaatatcggt gactacaaag
    14941 atcattctaa gtatctgcgc gaatgcatca aaatgatgga taggcagggc aaaatcattt
    15001 ccgaaatact ggagcttgtc agcctgaatg atgggagaat cgtacccata gctgaaccgt
    15061 tggacatagg gcgcacggtt gccgagttgc tgcccgattt tcaaaccttg gcagaggcaa
    15121 acaaccagcg gttcgtcaca gatattccag ccgggcaaat tgtcctgtcc gatccgaggc
    15181 tgctccaaaa ggcactatcc aatgtcatat tgaatgcagt tcagaacacg ccgcagggag
    15241 gcgaggtacg gatatggagt gagcctggtg ctgaaaaatg ccgccttttt gttttgaaca
    15301 tgggcgttca cattgatgat actgcgcttc caaggctgtt caccccattc tatcgcattg
    15361 atcaggcgcg aagcagaaaa agtgggcgaa gcagaaaaag tgggcgaagc ggtttaggac
    15421 ttgccatcgt acaaaaaacg ctggatgcca tgagcctcca gtatgcgctg gaaaacacct
    15481 cggatggcgt tttgttctgg ctggatttac cgctcacatc aacattgtaa atatttaaaa
    15541 cttaaatgat tttgggctct ttgtcaaata ggactgatag agtgagataa gaacatctaa
    15601 ggtagaattc cttttctact ttagatgttt tttgattcat aggtaatcag attatttagt
    15661 aagaaaattc tggtcttcat tttccgaaag cttcgaaagc cataagcgac tcgtttcaat
    15721 gctttgatgt gggtatgcag attttctatt ttggcatttg aataaggcaa ttcgagggct
    15781 aaacgaatgc cggattcata gtttataaaa gcttttttta tagaatgcag ttcttttgga
    15841 acggaattgg gcatcgttct tacaagttta aagaaagtgt ccgcctcttt gtcgtgaaac
    15901 gcttgaagaa tttcttgata gtaaaaatag gcgattcgta attcgtcaga gatcgaaagc
    15961 agacgatcca ccacttcact ttcggtgaga tacgcccaac caaaattgcg ccatttggtc
    16021 catttgtcat aaacaagctt gtcctgtcgt ttgatcagaa gccgagacaa cgcctttaat
    16081 tgtcgataat atttggcttg tgttggatcg tttcgatcaa agcttttcat gattcgtacg
    16141 cgaatatcct caaatttacg aatgagatgt ttgacaatgt gaaaacgatc cacaacgagt
    16201 tttgcctttg gaaaacattc ctttatcaga gaggcataag aaacattcat gtccgtcacg
    16261 atgaccttta ccttcaatcg tgcgcgtcgt tcaaattgca taaaataaga aacgagagcc
    16321 tttttcttcc gtgaaggaag aatatccagt aattttccag tatcgccatc catacaagaa
    16381 aaggagtagg gaccgacttg atttttcagt gaacgaaatt catccattaa taaacaacgc
    16441 ggtaaccaat ttctttttac ttttgtcatg gggatcagag aagcgagtgt ccgagaaacg
    16501 gtcgaccaag aaacgccgca atgcttagca attgtcgtca ttgcttgttt ttcagttagc
    16561 tcttctaaaa ttttatagcg cacttgttta gcgataaagc agtttctatc gacaatgtag
    16621 gtattcgccg taaaataggt gtggcagcca cgacagtggt agcgttgttt cgctaaagca
    16681 agataagtca gttggttcgc acatttatta agttgaatga gcgatttttt ctttccgttc
    16741 ttaacgatca tgccttcatg attcatctgt ttacacaaag gacagcgacg aatttcttta
    16801 cttaacgtcg cagaaacaac aagactcttg atattcttaa tgctcttcga ttccacattt
    16861 gtgatgaaaa tgtttttatc tttgattcca agtagtagtt tgatatgatg aagatcaggc
    16921 atacagaacg ctcctttgat tgtgttgtgg tgattcaatt ctaacggagc ttctgtatgt
    16981 tttgtttttt catgctcaaa agggaccgat agaatgattt tctatcagtc ctagaaatta
    17041 tagagccgat tttgaccgac aggtataacc ctgccggtct ttttttgctg ctac
//
LOCUS       DAYYCW010000272        16406 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.guided.066, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000272 DAYYCW010000000
VERSION     DAYYCW010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.guided.066"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(277..1617)
                     /locus_tag="ACHAYA_003076"
     CDS             complement(277..1617)
                     /locus_tag="ACHAYA_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8606776.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1886..2782
                     /locus_tag="ACHAYA_003077"
     CDS             1886..2782
                     /locus_tag="ACHAYA_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8606777.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(2932..3873)
                     /locus_tag="ACHAYA_003078"
     CDS             complement(2932..3873)
                     /locus_tag="ACHAYA_003078"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8606778.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            3999..5120
                     /locus_tag="ACHAYA_003079"
     CDS             3999..5120
                     /locus_tag="ACHAYA_003079"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8606779.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5378..6880
                     /gene="eat(A)"
                     /locus_tag="ACHAYA_003080"
     CDS             5378..6880
                     /gene="eat(A)"
                     /locus_tag="ACHAYA_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8606780.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            6947..7474
                     /locus_tag="ACHAYA_003081"
     CDS             6947..7474
                     /locus_tag="ACHAYA_003081"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8606781.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            7685..8746
                     /locus_tag="ACHAYA_003082"
     CDS             7685..8746
                     /locus_tag="ACHAYA_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8606782.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            8749..9645
                     /gene="murQ"
                     /locus_tag="ACHAYA_003083"
     CDS             8749..9645
                     /gene="murQ"
                     /locus_tag="ACHAYA_003083"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8606783.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            9659..11641
                     /locus_tag="ACHAYA_003084"
     CDS             9659..11641
                     /locus_tag="ACHAYA_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8606784.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(11706..12152)
                     /gene="nrdI"
                     /locus_tag="ACHAYA_003085"
     CDS             complement(11706..12152)
                     /gene="nrdI"
                     /locus_tag="ACHAYA_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8606785.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12363..13787
                     /locus_tag="ACHAYA_003086"
     CDS             12363..13787
                     /locus_tag="ACHAYA_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM8606786.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(13968..14741)
                     /locus_tag="ACHAYA_003087"
     CDS             complement(13968..14741)
                     /locus_tag="ACHAYA_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8606787.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14738..15643)
                     /locus_tag="ACHAYA_003088"
     CDS             complement(14738..15643)
                     /locus_tag="ACHAYA_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8606788.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(15797..16117)
                     /locus_tag="ACHAYA_003089"
     CDS             complement(15797..16117)
                     /locus_tag="ACHAYA_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM8606789.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         5323 a         2979 c         3386 g         4718 t
ORIGIN      
        1 ccaaacataa ctaccgttga aacaccgatc atcaacaaaa acttccaata ctttttcatt
       61 taaaacaaac tccttttttt cattcctttc ttctatcatg tcatgagcat ggagaacaat
      121 caattatttt gcacagaggg tttttgaaca ccgtttacca agaggtcatg agactgttta
      181 ttcggaaaaa ggagttgcaa caagactttt gtcacaactc ctttcttcaa tagctccatc
      241 tattccacat aaataaaata aaattttcta tcgatttcat tcattcgtca atacgaacgc
      301 atcattcttt ttttgataac tgaaataaat gaacaggtaa agcaggccag tcaatacagt
      361 taccccaatg gagttcgtga acaggatacc aaggaaaatc aatcctaaga cagctaatgc
      421 aaaaaagctg attcctttat cccataatgt tccttttctt agtgccagta caaaggatgc
      481 cgcactgata atgatccaga tcaataatac tgtatacgaa agggaacctg ccagatagcc
      541 aaataatttg tcaccgacaa aataagaaag agctacacca agataaagaa cactggtaca
      601 aaataaaaca gcacgttgcg gtacttgatg cttgtttaaa acagctagtt ttctagagga
      661 accttttcga tccttcaaac ggaagaacaa aattcttgaa gatgcataca cacccgaatt
      721 gatagatgag aacaaagcaa ggacaatcac aaaattgacg atatccgctg caaatgggat
      781 attcattttt tcaaatacca tcacaaaagg actgacattc gtgcctgcta aagtattcca
      841 aggaaaaaga atcaaaagca aaaacaaagg aatgatgtaa aaggaaatga ttcttcccat
      901 gacacctttg attgctttag gaatcgcacg tttaggatca tctgcttcgc ttaccgtgat
      961 agcaatcagt tcagaaccgc cgtaagaata gataactaca agcaaagaac tgatgattcc
     1021 cttcatacca tgaggagtaa acccaccatg gtcagtcaat tgctgaaggg tagggaaaac
     1081 gcctgtgccc aacatttgct gaccgacaag ataagtagca aaaatgatca gtaagataat
     1141 gacactgatt ttagcaaaag ccagccaata ttctgtttct gcgaataacc gtaccgaata
     1201 aagattgatg atcgtagtta aaacagccaa cataaaaaca aatacccagg ctggaatgtg
     1261 cgggaaccac aactgcaaaa aggaagcagc tgcaacagct tcagcgatga tattgataat
     1321 ccacatagac cagtagaccc aatcagtaaa atcagcagaa tgagcaccta gataagggcg
     1381 tatcaaacca gataatccgt gatgcgtgtc gccaccgctt aaaacgattt ttcccagacc
     1441 tttcatcacg atatataaaa tcagcccacc aatcaaaaaa gcaatcaata cagaagggcc
     1501 ggcgatccca atcgcttcgc cacttccttt aaatagacct gcaccgattg ctccacctaa
     1561 agctaacatg gtgatatgtc tggagctaag ttgttttttc aattttttat cttccataaa
     1621 tgacctcttt cttttaaatt cacaatcaaa atttatatta agaataaata gcaatattta
     1681 acaatgacta atgataacaa attttcagac aattacaact attcagaaaa cttttaggcg
     1741 aattttaata gaagaatgat ttatttcatc atattttcat acaaatgtta aattttcata
     1801 caaatatagg aaacgtttta ctggcttttt actatttaaa caggctatca taggaaaaag
     1861 tgacagagaa agtgaggcgg aaaagatgaa caaaaatgac gtactgctag ttgcaaaaaa
     1921 taaatcactt tattttttgc cttccatccg ctctttagat gtttggtccg gaaaagattt
     1981 actacagtcg gaattcatta aatcagctag acaacagttg gcaaattatc agctgatcgt
     2041 ttttttagat tatggatttg atatggagat ggctcgacgc gtgcgcccat ttaccaatgc
     2101 aaaaattgtg ttattttttt ggaatcactt caaagcagaa cacgaggcga tgctgaagca
     2161 ggcacaagat gaaccggcaa tcgatgagat ttatcatttt gattttttgg aagcaaaaga
     2221 acttgggctg aaacataata gttcatttta ttcgaaacat atgaattttt cttctagcga
     2281 accaaagacg gatctatttt ttggtgcaac gaataatggg agaaaagaac gagcagaaag
     2341 ctataagcag gaatttataa aaagagatgt tacgaccaac tatttcattc ttccacatcg
     2401 agggaatgaa caacctggat atcttactta tgaagagtac ctgaaaaaaa caggggaatc
     2461 gcgcgggatt ctggaactgt tgcgggaggg tcagcaagga gtgactttgc ggacatttga
     2521 atccatgtat tttcaaaaga aactagtgac ggataatcaa gcgattgctc attatcattt
     2581 ttatcgtcct gaaaatattt ttttgcttcg agaacgtgat ttagatgaat taccggaatt
     2641 tctgcataca ccatttaagc caatcctatc agagctattg gaattttatg atgcagaaaa
     2701 ttgggcaaaa cgatttatca ctaacgattc cactatttat gagcaatacg aatatgacct
     2761 ctcctctgtt gaaaacaaat aaaaaaccta cattttgaag agattatgta ctgaaacaaa
     2821 caagcacaat tcttatggca tcatgagagt tgtgttttct ttttgatctt ttattgggct
     2881 taaaaaaaga gaaccagctg ctgattcagc tgattctcat cagagagcct tttattctgc
     2941 gccaaggatt ccttcttttg catggactaa ttgggtcaaa aaggcacaat atggtgtagc
     3001 aatatcatat ttttgtcctt ttcgccaaat ggcaccattg atgtagtcga tttcagtcaa
     3061 acgatggttt ttgatcaggt cttggtacat agatgggtaa tgcaacccga tgttgtcagg
     3121 atcataagta gaagcaatat gttggtacac ttcttcttga tctagtatga ttccttcttt
     3181 tgctgcgata gaagcaaatt cagatacgat tgttcgaacc atgctctctg ccgctttttg
     3241 cgcgcctagt tcagcaatat tacaatccaa aatcgtgcat aaaccattca atgttccatt
     3301 tacacaagct tttcgccaaa tggaataacg aacgttatgg ctatagatcg gatttaaatt
     3361 tgcttcttga aagacttcta caactttttt tgtaaatgct tcgccttctg gttccaagtt
     3421 ctctagttcg atttctccat cacctaacaa tgtgactttt ccgggaccag ctagaccagc
     3481 tgtccacatc gtgatcccaa ataagatatt tttctttggc acatattttt ccagtacatc
     3541 ttcatgacct aatccattta gtaaacacaa aacataagta ttgtcagtaa taatggattg
     3601 gatcgaacaa aacatatcat ctaattgatt ggcttttgtt aatgctacga tcaagtccac
     3661 gtgttcgttg ctttcgatga tttcttctgg tgaatagatt ggcagttttg cgaccacttc
     3721 tttaccgtta aagtcagcaa tcaacccatt tgtacgaatg gcttcaatgt gagcaggcca
     3781 acgatcgatc aagaggacct catttccgct ttgatgtagc atcaatccga ttcggctgcc
     3841 cattgctccg gccccagcta ttgcgatttt catgtttttt ctctccttta ttcaatgagt
     3901 tcccttttag tatatagaaa aatggaagaa aatagcattg ttttgtttga aaaaaatgaa
     3961 aaaaagtgtc ttacttaaga agaaagtgag ggatacagat ggatattttg gcggcaattg
     4021 attcatttaa aggaagtgct tcttcatttg aactaaatca ggcagttcta aatggctggg
     4081 aagatcgtaa aggaagaaaa atcaatgtgc cgattgcaga tggcggagaa aatactgcag
     4141 aagcaatcta ttacgcgctc aggggaacat ggcgtaccat tgatgggatg gatctcttat
     4201 tccgtcagcg aaaaatcagc tatttattga caacttggca aggaaaaaac accgcagtca
     4261 tcgaatctgc acaagcattg gggttagatc tacttgataa accaacggat caaactatcc
     4321 gacttgcttc cagttatgcc ttaggagaac ttattaaaga tgcactgctt caagaagtag
     4381 aacaaattat tgtcacttta ggaggaagcg ggtgtagcga tggtggtcta ggattactcc
     4441 aatccttggg tgcttcttta gaagggtaca cagaaggaaa tcctttatta tctactaaaa
     4501 aacttcattt tgatcgaaga aacgttcttg tttctaaagt aaaattgatt atcgctgcag
     4561 atgttaccaa tccatataca ggtcaacaag gagctgcttg gcaatttgga agacaaaaag
     4621 gaggaataga tacgacgcta gctttcttag ataaacaagc aggaaagatt gctcagcaaa
     4681 taaaagattt ctgggaggtg gatttagatg atattccagg aagtggagca gcaggcggta
     4741 ttggtggagc cctttttttg ttgggagcga agatggtttc tggtttcgat ttagtcagcc
     4801 aaatcactgg attggaaaaa aagatcgaac aagcagatgt catcattaca ggagaagggc
     4861 ggatcgatca gcaaacgatc cacggaaaag ttccatacgg tgtagcagtg cttgcaaaaa
     4921 aatatcgaaa accaataatc gctatttgcg gaagtagaga ccaagagcta gaaggacttt
     4981 ctgattttct cccaattgtc ttaagtattc atttgggtcc aataggttta gaagaagcaa
     5041 tggaacacag gcaaacatta gaaaaaacaa aaatcttagg acagacactt agcagatttt
     5101 tcaccatcct cccgaagtag ttgacaaaat cattttcttt atgctatcgt aatttcaaat
     5161 ataggacaaa gtgagggatc cgtgcattga aaaattaagt cgattgatga aagctgcatt
     5221 cagactgtac cttgtggaga caaggaagca gtgtgcagat tttcgggatt ggcttggttt
     5281 tttcgatgta tgggtctttt tgtgtgtatc caacccttgc atcctcttta gccagttctg
     5341 aaaataggac tggtcttttt ttacgaggtg atggaaaatg tctaaaatcg aaataaaaaa
     5401 tctgacattc ggctacgaca gccaaggcac attattattt gaacaagcaa atctaaattt
     5461 tgacacacaa tggaaactag gacttatcgg acgaaacggt cgaggaaaga caactttact
     5521 gaatattcta caaaacaaac taccttatca agggcaagta atccatcagc aagaatttgc
     5581 ctatttcccg caacagacaa aagataaaga acgtttaacc tattacgtgt taaatgatat
     5641 tacggatttt gagatatggg aaatcgaaag agagctccaa ttgatgcaaa cagatcctga
     5701 aatcttatgg agagaattca gcacactatc ggggggagag aagacaaaag tcctactggc
     5761 acttttattt gtggatgaca ctcatttccc gttaatcgat gaaccaacga atcatttgga
     5821 tatctctggt agaaaacaag tagcggctta tttgaaaaag aaaaaacaag gcttcatcgt
     5881 ggtcagccat gaccggggat ttatcgatga agtagtggac catgttttag caatcgaaaa
     5941 aagtcaactg gaactttatc aagggaattt ctctatctat gaagaacaga aaaaacttcg
     6001 tgatgaattt gaaatggctc aaaatgaaaa attgaaaaaa gaagtcagta ggctaaagaa
     6061 aacagcagct gaaaaagccg aatggtctcg ttcccgagaa ggagataaaa caaagaaaca
     6121 agtcggattc atcgatactg aatctagacg agtgaataaa ggagcagtgg gtgctgatgc
     6181 tgcacggacg atgaaacgat ccaaagcaat cgtgaatcgg atggagaccc agatcagcga
     6241 gaaagaaaaa ctattaaaag atatcgaata tatcgattct ttgacgatga atagccaagc
     6301 gtctcaccat aagcgacttt taagcgtaga agatcttcaa ttagggtatg aaaatctgtt
     6361 attcgagcca attcatttta caatcgagcc tcatcagcgg gtggcgattt caggtcctaa
     6421 cggtgcagga aagtcatcca ttatccatta tcttctgggg gcattcaacg gcaaggttat
     6481 aggagaaaaa taccagccaa aacatctgag cattagttat gcaagccaaa attatgaaga
     6541 caatcgagga acgttggcgg aatttgcaga gaaaaaccaa gtagactacc aagcattttt
     6601 gaacaacctc cgaaagcttg ggatggaaag agatgttttt cataacaaga tcgagcagat
     6661 gagtatgggc caacggaaaa aagtggaatt ggctaaatct ttatcacagc cagctgaact
     6721 atatacatgg gatgaaccat tgaattattt ggatgtcttc aatcaagaac aattagaaca
     6781 actgatcttg aacgtgaaac ctgccatgtt actagtggaa catgatcaaa ccttcctgga
     6841 taaagtatct actgagatta tttctcttga gagaatctaa cccttgtaat ctcaagaaca
     6901 acaaggtatg gttaagggga actttgccaa cgaaggagag aatcacatgc tttatgtaac
     6961 gagacacgga gaaacaactt ggaacgcaca aggattagtt tgcggacggg cagatgtgcc
     7021 gctgactgaa aaaggacaga tgcaggcaca gaaactagca gaaaaggtag tagatttgcc
     7081 tgttccaatc acgaaaatca tccattcccc tcttcaacga gcaagagata cagcacaagc
     7141 agttgctgat cgtctcagct tgccgctgac cgttgatgaa cgattggtag aaatggattt
     7201 tggggattat gatggactgc caagcaaaga tgagaacttt caaaaagcac gtttagcgtt
     7261 cgctgtacga ttcccaaatg gggagtcagt cttagatgtg tatgcacgga tcgttccttt
     7321 attgaaagaa tgcatcgaag acgaagaaaa tgtctatctg ttagtttgcc ataatgcctt
     7381 gataagagtg atcaatgctt attttcatcc gatgcctaat gaggggttct ttacattcat
     7441 ggtagataat acagaattgg tttcttatga gtaattatgt ttttgtgtta tttaaataaa
     7501 atatttcaag gcactgcttt tgcttagcag tgcctttttt aggttatgac aattcttttg
     7561 ataatataaa ctcaaccttt agaaatgaaa tttaattatt ttaataaaag tattgaaatt
     7621 ttgtttcttt agatatatac tctagtcata gaaatgaaga cggatacaga aaaagaggga
     7681 taatatgttt ggcttttcaa tatttatgaa cgattctttg tcagaagaga agaaacagta
     7741 tatccgccaa atggcatcca atggctttgt cggtattttc acttccatgc atataccaga
     7801 agatgatgtt tcggcatatc aaaagcgatt gatcgaacta ggaagcgaag cgaaaaagaa
     7861 taaactagaa ttgatggtcg atatttcagg agaggcattg gaacgagcgg gtttttcatt
     7921 agaagatcta acgcctttga aagcaattgg tgtaacaggt ttaagaatgg attatcatat
     7981 ttccaatcag cagattgccg actggtcaca tcaattgaaa atcagtttga atgctagcac
     8041 gatcacgcct aaagatatcg atgaattgaa agaagcagaa gctgatttct cacagattga
     8101 agcttggcat aattattatc cacgtcccga aacagggctg gacaaagaat ggtatcagaa
     8161 aaaaaatcaa tggctcaaaa ttcaaggtct ccttgtccaa ggttttgtac caggagatac
     8221 agagttgaga ggcccactat atcaaggatt gccaactttg gaagaacatc gagggatcca
     8281 tcctcttgca ggcgctctag atctattagc aagtaacacg gatattgttt acatcggaga
     8341 tgcaggtctg tcagaaaacg ttcaggagca atttgcttct ttccaaaaag aacagacagt
     8401 cttgctccat acggaacctg tagatgaaga attttacgaa tatatattag ggaagcatac
     8461 gaatcggcaa gacgatgcaa gagatgtcat acgaagcgta gatgcacgtt ttcgagagat
     8521 tccgcctatc ccagcaagaa atacagctac gcgtatgaaa gggtctatta ctttagataa
     8581 cgaaaagtat ttgcgctata tgggtgagat ccaattaacc aaatatgatt tgccagcgga
     8641 tgaaaaagtg aatgtagtag ctaaagttat caaagaagag ctaccattga tcaaccaaat
     8701 caaagctggt atgaactatc aatttataag aaaagaagga agataaaaat gagcaaaatc
     8761 aatttagaga acctaacaac ggaacgcaga aaccaagaaa cttttggatt agatgagatg
     8821 agtatttctc aagccgttca actaatgaac aaagaagatc agaaagtggc cgctgcggtt
     8881 gaaaaagaac tgacaagtat cgaaccagtg attgctaaca cgattgagtc ctttaaaaaa
     8941 ggtggacgtc tgatttatat gggagccgga acaagtggaa gactaggtgt gttagatgct
     9001 gctgaatgcg taccgacatt cggtgtagaa ccggaaatgg tcataggtct aattgctggt
     9061 ggagaagaag caatgactgt agctgtcgaa ggggccgaag attcttatga gctagggcaa
     9121 gaagatctaa tcaagctgaa tttgacaaaa aatgatatag ttattggtat cgcagctagc
     9181 ggtcgtacac catatgtgat cggtggatta gattacgcaa aatcgatcgg tgctgtcaca
     9241 ggaacgattt cttgcaataa acaagccgaa atcagtaaac acgcggattt cccaatcgaa
     9301 gtcgatgcag gtccagaatt tttgacaggc tctactcgat tgaagtcagg aacagcacaa
     9361 aaacttatct tgaatatgat ttctactatc agtatgattg gtataggtaa agtattcaat
     9421 aacttgatgg tcgatgtcaa accaacaaat gaaaaactaa tcgaacgctc aaaacgaatc
     9481 atcatgcaag cgacagaagc tgattacgaa acagctgaaa agtactttga agaagcagat
     9541 caaaatgtta agctggctat cgtcatgatt ttgacaaata gtgataaaga ggaagctcaa
     9601 gcaaaattag ctgaagcaaa cggatttgta aaagggacct tatgaggggg aaaagaccat
     9661 ggcaaaaaac gaactgactt tggaagaact ggcaaaacaa atttataaag gggcaggcgg
     9721 catgggcaat gtagaaagca ttgtccactg catgactcgt gtgcgtatga agatcatcaa
     9781 tgatgatctt gtaaatgtct ctgaattaaa agcgattcca ggagtgcttg gtgtagtaga
     9841 agatgaacag cttcaagtca tcatcggacc tgggaaagtg aacaaagtag cacagcaaat
     9901 ggttgatttg gcaggcgtga aattaggaga aaaactttct tctgtaagta cagctacagc
     9961 aaattcatca gcatctggaa aagaccgtgt aaacgaacgg gcacaagaaa tgaaagcggc
    10021 acaaaaatcg aaacaaaaac catctaaaat caaatcggta ttaaaagaca tttctaatat
    10081 ctttgtgcca atgattcctg catttgtcgg tactgggatc gtggcaggga ttgccgcagt
    10141 tttagcaaac ttagtcacag ccggaacact tgatgcagca acatggcaac aatatatcga
    10201 tgtgatgaac atcttgaaaa acgggatgtt tagctaccta gttatctata caggtatcaa
    10261 tgctgcacaa gtttttggtg caacaccaac tcttggtgga gttatcgggg ccgttgtgat
    10321 gctgacaggg atgaatccgg aagcaccact gaaaaactta tttactggtg aagctcttgc
    10381 agctggacaa ggtgggatcc taggtgtcat cttcgctgtt tggctattgt caatcgttga
    10441 gaaaaaactg cataaagtag tacctgatgc ggtagatatc atcgttacac caactctttc
    10501 attgattgcc atcggtctga ttgaaatttt cttcatcatg cctttagcag gtttcatttc
    10561 tgatggcatg gtcggtggga tcaactgggt acttggcgtt ggtggagcat ttgccggctt
    10621 cgttctaggc acattattct tgccgatggt tatgttcgga ctgcatcaaa ttttgactcc
    10681 aatccatgtg caaatgattg atgaaactgg tcgtacgcta ttgcttccaa ttcttgcgat
    10741 ggctggtgca ggacaagtgg gtgcagcatt ggcattatgg attcgctgca aaaaagataa
    10801 agaattaaca gaaatgatca aaggagcttt accagtcggg atcctaggaa tcggcgaacc
    10861 attgatttac ggagtaacat tacctctagg acgcccattt attactgctt gtatcggtgg
    10921 aggtatcggc ggggcagtga tcggaatgat cggaaatgtc ggggctatcg ctattggacc
    10981 ttcaggagcc gctttgatcc cattgatctc tgacggtaaa tggttaggct acatccttgg
    11041 attattggca gcttatgctg gtggcttcgt tgctacattc ttcttcggta ttcctaaaga
    11101 ccgtttagaa aaagaagaat tagcgactga aacagccaca gtaaatgaag tgcctgaaaa
    11161 agtagtacct caaacttcag ctgaaagtgt gactatcctc tcatcagttg ctgacggaac
    11221 agttgtttct ttagataaag caagtgatcc agtttttgca caaaagatga tgggtgaagg
    11281 ctactttgta gaaccagaaa acggacatat ctattctcca gttactgggg tagtcagctc
    11341 tgtcttccca acgaaacacg ctattgggat cacaacgcca aatggtttag aaatcttgct
    11401 tcatatggga atcaacacag ttgacttagg cggtacgcca tttgatgtga aagtaacaga
    11461 agggcagcaa gtgacacctg atacgcttgt agcagaagct gacttagaag cgatcaaagc
    11521 agcaggcaaa gaaacatcca tgatcgtctt ggtaaccaat atggatcgtg taaaaaactt
    11581 tgtcttggaa aaaacaggta aagcaaaagc aaaagcacct gtgatggatg tcaaagctta
    11641 atatgataga aacaatagaa acgaaagaag aaactgaaat ctatttttca gcttcttctt
    11701 ttttattatt tattgttttc tgctaaaatc tgataaattc gtgtcacatc agatggtgtt
    11761 ccccgtaatt cataatctgc caagaaagga aagccaaatg tttgtgcata ttgtttggct
    11821 gttagacaat actgattatt gaagttttta tttccgctac cgactacacc aagacaaagt
    11881 ttatgattgt cttcatgttc cagatattca cgcatcgttt cggtcaagat ttctgtatca
    11941 ccgttatcta aaccatttcc gccgtctaaa taagtaggaa cgaaagtaaa aaatggttct
    12001 gtttctttcg taaaatcact attttcatgg atttctttta aagtaacttc aggattgaca
    12061 ggatcttctg catgcatttt ttcagcatat tctgctagat gtttggcaaa ggcacgcgta
    12121 tttcctgaaa tcgatatata taaaatgttc atggatcatc ctccaattct ttccttttag
    12181 tatagcatct tttttaagtt ttttggtggt gtaatttgga aagggactag gcgctttgct
    12241 tgtataatga gaaaaaagcc ggtagaatta tatttctatt aaagttttta ttaattaagt
    12301 aaaaataaaa aaggtttgtt atactttttg ataaaagcgg ttttattttg gaggatgctt
    12361 acatggatac agagcaacag aaaaaaggaa ttggtttaat tccacttgct gccttagtaa
    12421 ttggttcagc gattggaggt ggcgtatttg gtattatgac ggacatctcc ggatcggctg
    12481 gcggcccagc tattttcagt tgggtactcg taggaacagc aatgttgatg ctctccttgt
    12541 cgatcaataa tatcaataaa aaacgtccag atttaaaagg cgggatcttt agttacgcag
    12601 aagaaggttt tggccgtttc gctggtttta tcagcggttg gggttactgg ctcacatgct
    12661 ggttaggcaa cgtagctttt gcaacattgt tgatgagcgc attaggatat ttcttcccag
    12721 tatttgaagg tgggcaaaat ctggcatcga tcgtgatgag tacagtcttt ttatggggat
    12781 atgcatggct agtgaatcgg ggagtcgaaa atgctagttt cgtcaatgct atcgtcacat
    12841 tctgtaaatt agtgccatta tttttattta ttgtagtagc tatcagcacg ttttcgatcc
    12901 aacaattcat tgataacttt tctagcgaca ttatattgaa tgcgaatgga cagccgatcc
    12961 cgttttccag tcaagtattg agttgcatca tggtgatgct ttgggtattt gttggtattg
    13021 aaggagcctc tgtcgtaggt tcacgagcaa aaaaacaatc agatgttggg aaagcaacga
    13081 ttctagggat attcggcttg attattattt acgtcttggt ttccatgctt ccatatggga
    13141 caatgacgat gaatcagctg caaacgatca aggcgcagcc tgctatgggc tatgtgtttg
    13201 aaatgatggt tggtaaatgg ggagcagtcg tgatcaacgg cggtttgatc atttctttag
    13261 ttggtgtttg gctctcatgg acgatcttac cgattgaaac aatgggaaac atggctgaag
    13321 acggactttt gcctaaagca tggggaaaac tcaacaaaaa tggggcacca acttatgcta
    13381 tcgtattgac gacgttatgt acgaatgtct ttttactttc attattattc actgacagtg
    13441 cttataactt tgcttacaca cttggtacag cagctatttt cttcacgtgg cttttcttag
    13501 gattgtacca aatgaaactt tcttatcagc ggaaagaatg ggtccaattt ttagtagggc
    13561 ttttagcaag tgcctttcaa gtatgggcta tgctagtcgt agcctttaat gaagtaatgc
    13621 ttgttcttgt tcttttcttg ccaggcattt atttttacct caaagcacgt aaggagcaaa
    13681 acgagcctgt acaacatatc cacttagata agattttact tataggtgtg acgatggtcg
    13741 gagtgattgc aatggtttta tttgccactg ggatgcttca cgtatagaag tataaaagaa
    13801 gtataaagag aggttgtgag cctgtcgttt attcaaaaac atactaggaa aggagctgtg
    13861 acacaaatgt tgtcacagct cctttttcag aataaacggt gtttaagaga cagccaatcg
    13921 gcaataaggc tgaacgtctc tttcacagcc tcttttttga tgagtcatca tacatttttt
    13981 cgcttaaata cgagatatcc taatgctaag aacaaaacga tgtaagctaa attaccgaat
    14041 agcatttgat tagtagaaag atgagtcaat tgcttcatga tctcttcgtt tccaatttgg
    14101 ttttgcagat tcaacatact gatcggattc catttgatcc actcccattt ttggatgagt
    14161 gcaataagga taccagatat gattgacgaa gcaaaataaa agacgattcc tgctgagatg
    14221 gatgcgccgg aactattgat aaagcaagca atcatcaaca ctaaacttaa aatcagccat
    14281 aaaccgatat aactacccaa agtataagtg aacaagcttt ggatcagtgt ttgaccagta
    14341 gaaaccttct ccgtgaagga aatcgaaggg aaaagaatca gtttcatcaa cacagtgaca
    14401 ataatagcta aaaggtataa ataaacagaa taaataacta atgtgatcca tttgcttact
    14461 aatatttctc ctcgagaata ctttcgataa aggagatttt tcaatgtacc atattgtgct
    14521 tccatcgaaa tgatcgtgct tgcagctgca atcatgataa agacaatcca agatgtggca
    14581 ctaaaaagct gagcagtcat ttcaactgga tcaatgatcg tcgtgtattt tttggctaga
    14641 agtgctgttc ctatgagcaa aacaactaat agaacagata aaacagcagt tgactttttc
    14701 ttgatcaatt taaataattc ttgttttaca agcaatgtca tttttcttct tcccccttta
    14761 ctgaatccag aatttccaat agagaagatt ccaaatcatt cgtcagatgc tggacgtctg
    14821 tcacatggat attttgtgca gacaatagct ggatcacggt tcctaataat tcttcgtttt
    14881 cttttaaaag cttgatttta tcaggatctt ccaaagtata tccagcttct gtcaagatcg
    14941 ttcgagcagc tagatcattt gttgtagaga taacgatgaa atcttgcgct gtttggttca
    15001 attcagccat tgtacattgt ttgatgatct ttccttgatt gatcaggatc agctcatcga
    15061 ccattttttc aagctcgctg agtaagtgac tggagatcaa gaaagaggtg ccctgagtag
    15121 ccaagtcggt aatgattttt cgcacatctc ttgttgcttg tggatccaaa ccgttcatcg
    15181 gctcatctaa aataacgata gacggatggt tcagtaaggc aagagcaatt cccaattttt
    15241 gtttcattcc caacgagtag gatttcgctt ttttgtgaat gtacttttcc atcttgagct
    15301 gattcacgat agtgtcgata gcagatacat cgttggtttc agagaataac ttcagatgat
    15361 catagcctgt taagaatgga tagatacccg ggtattcgat caatgctcct acttgttcca
    15421 accctttatg actagaggga gaaatctcat tttcaccaat acagattgtt ccatctgagt
    15481 aatggatcag tcctaaaata gctttcatga tcgttgtttt tccagcgcca ttaggaccta
    15541 ctaatccaac gattttgcca ggggataagc taaaactgac atcgctgagg gcttgtttgt
    15601 gaccaaagtt cttggataat tcttggaccg aaagactttt caaaaatatt cactcctttt
    15661 ctatagatag tacaagcata cacctgatga gtggagaagt cgctaggtca ttggacttat
    15721 ttcttatttt ttcataaaaa aattgaatat taaataaaaa ggcttatttt ttctgatcag
    15781 tacttattcc aagtgtctag tggccgctag taatcttgat gccaatgata ctgatgagta
    15841 gcatccccac aaagaaccaa gtggaaagat tcagcttatc atgaaacaag accacaccaa
    15901 ttatgaccga accaactgcg ccaatccctg tccagatagg ataagagaga ctcatcggca
    15961 aacttttgat tgcctttgac aaaaagtaaa aactagcaat catgccaatc actgtgtaaa
    16021 aagaataatt gattttagaa aaaccatcac tccatttcat catcgttgac cagaatactt
    16081 ccaaaattcc tgctatgatt aattccatcc atgccatact tacattcctc cttaactata
    16141 aaaaaataca caaaaagagg aaaatcatcc cttcaaagac ctaatggaat gatcatcctc
    16201 tttatcctac ccggtggcag attgtatatc tttttctgta gtatagcaca cctatccaga
    16261 tatcggcaat ctcatacttt ttagtaaagt gtaccaagca ctgacggata tatcgttagc
    16321 ggaaaagaag atgaatgctt tgcaatttca tagaaagaag ttgttattta ttaatcattt
    16381 aaaaaaaaca gtaactagac cttatt
//
LOCUS       DAYYCW010000273         4655 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.guided.152, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000273 DAYYCW010000000
VERSION     DAYYCW010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.guided.152"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..953
                     /locus_tag="ACHAYA_003090"
     CDS             147..953
                     /locus_tag="ACHAYA_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM8606790.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            971..1678
                     /locus_tag="ACHAYA_003091"
     CDS             971..1678
                     /locus_tag="ACHAYA_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM8606791.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            1675..2646
                     /locus_tag="ACHAYA_003092"
     CDS             1675..2646
                     /locus_tag="ACHAYA_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8606792.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2639..3667
                     /gene="vanB"
                     /locus_tag="ACHAYA_003093"
     CDS             2639..3667
                     /gene="vanB"
                     /locus_tag="ACHAYA_003093"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8606793.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3673..4281
                     /locus_tag="ACHAYA_003094"
     CDS             3673..4281
                     /locus_tag="ACHAYA_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8606794.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4347..4520)
                     /locus_tag="ACHAYA_003095"
     CDS             complement(4347..4520)
                     /locus_tag="ACHAYA_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606795.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1314 a          980 c         1236 g         1125 t
ORIGIN      
        1 accgacaggt ataaccctgc cggtcttttt ttgctgctac aggaaaacta cagactggct
       61 acagggaaag tacagatgcg cttgccataa taacaatcgt accagccaca aatcgtagtt
      121 ttattgcaaa ggaggcattc gatcaaatgg aaaaaagcaa ctatcattcc aatgcggatc
      181 atcacaaacg gcatatgaaa caatccgtgg aaaagcgggc ttttctatgt gcgttcatta
      241 tttcgttcac agtctgcacg ctgtttttgg ggtggagact ggcttccgta ttggaggcaa
      301 cacagatacc gcccatccct gcaactcata caggcagcag cactgacgta gtggagaatt
      361 tggaggaaaa cgctcttgcc accgccaaag aacagggaga tgaacaggaa tggagcctga
      421 ttttagtgaa caggcagaac cccatccccg cacagtacga tgtggagctt gagcaactat
      481 caaatggtga gcggatagat attcggattt ctccctatct tcaagatttg tttgatgccg
      541 caagaactga tggagtttac ccgattgtcg catccggata ccgaacaaca gaaaaacagc
      601 aagaaattat ggatgaaaaa attgccgaat ataaggcgaa aggctacacc tctgcacagg
      661 ctaaagcgga agcagaaact tgggtggccg tgccgggaac gagcgagcat cagcttggtc
      721 ttgctgtgga tatcaatgcg gacggaattc attcaacagg caacgaggtt tatagatggc
      781 tggatgaaaa cagctatcgt tttggtttta ttcgtcgcta cccaccagac aagacagaga
      841 taaccggtgt gagcaacgag ccgtggcatt accgatatgt tggcatcgaa gctgccacag
      901 agatgtacaa ccaaggggtt tgccttgagg aatatttaaa accagaaaaa tgagaaaagg
      961 atgttatgtt atggacagaa aaagattgac acagcgtttc ccgttcctgc ttccaatgag
     1021 acgggcgcag agaaaaatgt gcttttatgc gggaatgaga tttgacggct gtcgctatgc
     1081 gcagacgata ggggaaaaat cgctttccca tttgctcttt gaaacggatt gcgcattata
     1141 caaccacaat accggatttg acatgatata ccaggaaaac aaggtgttca acttaaagct
     1201 ggcagcaaag accttaaacg gcttattgat aagaccgggg gaaacctttt ctttctggtt
     1261 gttcctgcat acgccattga caattatcca gcgcagaggt cacgaagtaa aggagtttcc
     1321 agagccaaac agtgacgaga ttaaaggggt ggatgcaacc atttcagagg gctggattga
     1381 tttaaaagtg cgaaacgata ccgactgcac ctaccaaata tgggtgacct tagatgatga
     1441 gaaaatcatc ggtcaggtgt tcgccgacaa agagccccaa gcattataca aaattacaaa
     1501 tggcagtatc cagtatgtcc gtgaaagtgg cgggatttat gaatatgccc aggttaaacg
     1561 gatgcaagtt gccttaggta ccggggaaat aatagattgc aagctgcttt atacaaacaa
     1621 atgcaaaatc tgttatcccc tcccggaaag tgtggatatt caggaggaga accaatgaga
     1681 aaaagtgtgg gcattactgt ttttggatgc gaacaggatg aggcaaatgc tttccgcgct
     1741 ttatcgccgg attttcatat tatccctacg ctgattagcg atgcgatatc ggcagacaac
     1801 gcaaaattgg ccgctggcaa tcaatgcgtt agcgtaagcc ataagtccga ggtttccgag
     1861 gcgacaattc ttgcgctgag aaaggtcggg gtaaaataca tttctacccg cagcatcggc
     1921 tgcgatcaca ttgatacgac tgccgccgag agaatgggaa tctcggttgg cacggttgcg
     1981 tattcgccgg acagcgttgc ggattatgct ttgatgctga tgctgatggc catacggggt
     2041 gcaaaaccca ccatgcacgc cgtggcgcaa caagatttca gattggatcg tatccggggg
     2101 aaagaactgg gggatatgac tgtgggagtt attggaaccg gccatatcgg gcaagcggtc
     2161 gtcaaaaggc tgcggggatt tggatgccat gtgctggcct atgataacag ccgaaaaatg
     2221 gatgcagatt atgtccagct tgatgagctt ctaaaaaaca gcgatattgt tacgctccat
     2281 gtgccgcttt gtgcggatac ccgccatctg atcggtcaga agcaaattgg agagatgaag
     2341 caaggcgcat ttttaatcaa caccgggcgc ggagcgcttg tcgataccgg ggcgctggtg
     2401 gaggcgttgg aaagcggaaa gctgggtggt gcggcactgg atgtgttgga gggcgaggat
     2461 cagtttgttt ataccgattg ctcgcagaaa gtgcttgacc atcccttttt gtcgcagctt
     2521 ttaaggatgc cgaatgtgat catcacaccc catacggcgt actacactga gcgtgtgctg
     2581 caggatacta cagaaaaaac aatcaggaat tgtctcaatt ttgaaaggag tttacagcat
     2641 gaatagaata aaagtcgcaa tcatcttcgg cggttgctcg gaggaacatg atgtgtcggt
     2701 aaaagccgca atagaaattg ctgcgaacat tgatacggaa aaattcgatc cgcactacat
     2761 cggaattaca aaaaacggtg tatggaagct atgcaagaag ccatgtacgg aatgggaagc
     2821 cgacagtctc cccgccatac tctccccgga taggaaaacg catgggctgc ttgtcatgaa
     2881 agaaagcgaa tacgaaacac ggcgtattga tgtggctttc ccggttttgc atggcaaatg
     2941 cggggaggat ggtgcgatac aggggctgtt tgtattgtct ggtatcccct atgtgggctg
     3001 tgatattcaa agctccgcag cttgcatgga caaatcactg gcctacattc ttacaaaaaa
     3061 tgcgggcatc gccgttcccg aatttcaaat gattgataaa ggtgacaagc cggaggcggg
     3121 tgcgcttacc taccctgtct ttgtgaagcc ggcacggtca ggttcgtcct ttggcgtaac
     3181 caaagtaaac ggtacggaag aacttaacgc tgcgatagaa gcggcaggac aatatgatgg
     3241 aaaaatctta attgagcaag cgatttcggg ctgtgaggtc gggtgtgcgg tcatggggaa
     3301 cgaggatgat ttgattgtcg gcgaagtgga tcaaatccgg ctgagccacg gtatcttccg
     3361 catccatcag gaaaacgagc cggaaaaagg ctcagaaaat gcgatgatta cagttcccgc
     3421 agacattccg gtcgaggaac gaaatcgggt gcaggaaacg gcaaagaaag tatatcgggt
     3481 gcttggatgc agagggcttg cccgtgttga tctttttttg caggaggatg gcggcatcgt
     3541 tctaaatgag gtcaataccc tgcccggttt tacatcgtac agccgctacc cacgtatgat
     3601 ggccgccgca ggaatcacgc ttcctgcact gattgacagc ctgattacat tggcgttaaa
     3661 gaggtgataa gtatggaaaa tggttttttg tttttagatg aaatgttgca tggcgtccgt
     3721 tgggatgcca agtacgccac atgggataac ttcacaggaa aaccggtgga tgggtatgag
     3781 gtgaatcgca tcatcggcac aaaggccgtg gcgtttgctc tgcgcgaggc acaaatccat
     3841 gcggctgcgc ttggctatgg cttgctttta tgggatggat atcggccaag aactgcggtg
     3901 gactgcttcc tgcgttgggc agcgcaaccg gaggacaatc tcacaaaaga aaaattttac
     3961 cccaatatag agcgagccga gttgattaca aagggttatg tggcctcaca atccagccat
     4021 agccgtggaa gcgcaattga tcttacgctc taccacctgg atacagggga acttgtttca
     4081 atgggaagta acttcgattt tatggacgaa cggtcgcatc atacagcaaa agggataggg
     4141 gatgcagagg cacaaaatcg aagatgcttg cgtaaaatca tggaaagcag cggatttcag
     4201 tcttatcgct ttgaatggtg gcactataag ttgattgatg agccataccc cgatacctat
     4261 tttaattttg ccgtttcata atgaaagtat tggttttgta tcagattttg gatttgataa
     4321 aatctatgct ggtcatggac aatcttttac tggaaagtgg ttaaagcatc ttttgtaata
     4381 cattgcaaat tatagtattc aatctgccca gcggcagaaa agaaaaaaac cgctgttggg
     4441 cagacctttc ccacgcccat tttgtatttt gctggcggcg gttttgagaa atcaaggccg
     4501 ccgttctttt ctggcagcaa aaggtgtggc ggcccatagg aggatgccgc tatgctgttt
     4561 tgctattgcc atgctctaat ccacttccac cactcaaaaa aagccttgcc cctccatgaa
     4621 ctgaacccca aaagttgaac tatttaatgg actgt
//
LOCUS       DAYYCW010000274         4445 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.guided.156, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000274 DAYYCW010000000
VERSION     DAYYCW010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.guided.156"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            306..803
                     /gene="dfrG"
                     /locus_tag="ACHAYA_003096"
     CDS             306..803
                     /gene="dfrG"
                     /locus_tag="ACHAYA_003096"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8606796.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(875..2827)
                     /locus_tag="ACHAYA_003097"
     CDS             complement(875..2827)
                     /locus_tag="ACHAYA_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8606797.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2834..3070)
                     /locus_tag="ACHAYA_003098"
     CDS             complement(2834..3070)
                     /locus_tag="ACHAYA_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606798.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3472..3762
                     /locus_tag="ACHAYA_003099"
     CDS             3472..3762
                     /locus_tag="ACHAYA_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606799.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(4034..4383)
                     /locus_tag="ACHAYA_003100"
                     /pseudo
     CDS             complement(4034..4383)
                     /locus_tag="ACHAYA_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT         1417 a          740 c          769 g         1519 t
ORIGIN      
        1 aaattagtaa agaagcaaca gtaatctata ctaagtagtt cagttttgga gtacaaaatg
       61 acaaaatgta cttcaaaatg gactactttt ttcattttaa cttacatacc aaattacata
      121 caaaatgatt tataaaatgc aattgaaaat gataattgaa tgaaaatttg tgattttgtg
      181 gtattatgat aatataactt atagaaaaaa ttgcttattc aactaacggg caggataatg
      241 agaaaaggtt ttttgataga aataagaaat tagatggtaa attagttaaa aaggctgtgt
      301 tataaatgaa agtttctttg attgctgcga tggataagaa tagagtgatt ggcaaagaga
      361 atgacattcc ttggaggatt cccaaggact gggaatatgt taaaaatact acaaagggac
      421 atccgataat attaggtagg aagaaccttg aatcaatcgg aagagcctta cctgacagaa
      481 gaaatattat tctgacgaga gataaggggt ttacctttaa tggttgtgaa attgttcatt
      541 caatagaaga tgtttttgag ttatgtaaaa acgaagaaga aatttttatt ttcggaggag
      601 aacagattta taatttgttt ttcccttatg ttgagaaaat gtacatcaca aaaatacatc
      661 atgaattcga aggagatact ttttttccag aagtgaatta tgaggaatgg aatgaggtat
      721 ttgcccaaaa agggataaag aatgataaaa atccgtataa ctactatttt catgtatatg
      781 aaagaaaaaa cttattgagt taacgggtgc gttaattgaa gaagaacagt aaagataaag
      841 cacaagagct tgggattact ccaagctctt tttgtcattt gaaataaatt atatctttcc
      901 aatcaaattg tttatctgta atccctaatc tttgagcagg tgtcaaaacc tttttatccg
      961 ctgatttaaa agggcgacaa aagttgtagt aagttcttag aattgtaatc gcaaattgag
     1021 catacttcgg attgaagtta gcgtaaatat agctcttctt atcaccacga gctgttgtta
     1081 atggtctttc aagagatgat atataacgtc ttatctgttg aataaagcta ttggtcgaat
     1141 ggtcattaac atttaatagc atctttgcga tttcgtttgg ttccagagca gataaatccg
     1201 ttctacaatc aactgaataa aatcctttat ccttagtggc taatggatgt ttgatagggt
     1261 tatcggcata tttgcgatat ctttctccat ctttactaat agcctcttcg tggaattgat
     1321 gacttgttag taattcagtt agataatgag atgccaaagt ccgtagaaac tttgttttga
     1381 atcctctgat atctccccaa tcgagcagtt ctgcttttgc ttgtccgaat tcttttaagc
     1441 attgttttct gcttttcgtc ttgttaactt ggcaaataaa atgatgtgca tcgctcaacc
     1501 ttaattcttt cgtgaatact ctataaaagg cgtttctaat agagaagtca tcatcactaa
     1561 tcattcgcca ttcgtcggca ttaaccattt gtttaattaa ccaatagtga gcagttgtag
     1621 tataaggagc atcaacattt aaaccatcga caaatctact cctattagta attttcccta
     1681 attcgtgaag ataagcattc ctattttcgc tatcgttagc ggatggttct tgtggataat
     1741 aagaccaatc taacctatca tttttacgac taaaagtatt gagatggtct tctttgaatt
     1801 ttcttgtatc ttcatttaat tcatccattg aaatattcca atcataggca acatcagaac
     1861 ggaacacata tcttgataag acctcagcag atacaactat atacgtttgc atattaaggt
     1921 cttcaaaccc cgtatatttc tttgaacctt gtcctttctt acggacgtta tttaggtagt
     1981 agtgcatctt gtccgtatta agccacatct cgttaaattt ctttgtttga aggggttgtg
     2041 tctcatatcg ttctaaaaac tccaaacaac gcctatacag ccactccagc ttatgatagt
     2101 aggttccaac tccaattcct aaaatatcac aagtacgact gactgaaacc cttccaacaa
     2161 ccatcttagc gaacattgga agaatggtgt tcttttgttg gtgataagta gtggtttctt
     2221 ctctttttgg tagaacatta gtgaacttct tacacgcctt acattgatag cgttgagatt
     2281 tgcccctact tttccccctt ttgtaaaatt gcttcggctc attgaatggg gttgattctt
     2341 cttccgagca gccttcttta tgaaattgat aatctggctc aacatcttgg atgctattga
     2401 tttctataag gcgtttaatt tcttcaacaa cagaccaatt agataaaggg gttactgtac
     2461 agttttgcga tacacctctg ccaataggat tgtcattgca gtacatacct ttatgacctt
     2521 tatctttgga actaccagtc attttatatc gacttggttt cccttttaca tctttgaatt
     2581 tgtgctgttc tttcccaaaa ttacaacaaa acggattgat gcagtaatta tattgaatgg
     2641 gatattcaga accgttccat ttaaaagacg ttggcaagaa caacattttg ttataccttg
     2701 ttgcaaatcg ttttggagtt aataaatcat attgtttagc cctatctttt aattcttcgt
     2761 ttgaaatcgg aattttaaca tctacaattt catcttcctt cgtggctaat cttttaagct
     2821 tggtcattgt ctatcaaccc actctatctt tgatacccat tttttcaaca atccgcttat
     2881 tacttcgctt gttggcaatc cattcaataa acttcttatc gtctaaaatg ttcaataaga
     2941 attcatccac tgcttcgctt tcagtacgct ctgcatattc agcgtattgc ttaactatca
     3001 cccttacttg ctctgatatt tcccaatcca cagacttagc gttcttattt tttggctcaa
     3061 gaaattgcat aggaaaatgc accccttttt gcttagtaaa ttgtaaccat tttaacacac
     3121 caatttgcat tcctacaata attatttcac gttcgcattt ttatatacat tttaacttac
     3181 attttgtggt gtaaattaac aacaaacgtt gatttaacaa taaaaaacga gtatcaaatt
     3241 cagttgcaat ttgatactca tttaggttta cattttcact ttttgtactc caaaactgaa
     3301 ctacttaaat ctatactgct gcctcttttt tttagtacat gtccttcatc ttcgcaggaa
     3361 gtgccaatat gattttaaga aaggtaatag acaaaaagtc aaaaagataa aacagagttt
     3421 cttctttaca aagggaatgt atgttcgtat aatggtattg aggtgaaggt tatggagaaa
     3481 aaatatggaa cagtagataa gatccgtact ttgcaagtta gtgccagacc gttgatccgt
     3541 ttcagtttaa atggggtcaa ttgtttgatt gcttcccata gtttgaactt cctagctgaa
     3601 gtagacgaag ggatgaagtt agtcgttaca ggttattata ataatagaaa gcaatttgtt
     3661 gtgagatcct atcatgtgtt agggaaaccc aaaattgtgg tagaatatga gaaaagtcta
     3721 tacccaagaa aaaaggtccg acaagatgaa agaataaact agaaaacaag cggctgagac
     3781 aaaaatgtcc tagccgcttg ttttggaaag atggatgcag acgatgggat ttgaacccac
     3841 acgagcatac gctcacaaga acctcaatct atatataact acatgtattt aagtatttgt
     3901 gatgaatcac taaactttcc aaactcattt gagggatatt gcttaaagat tgaacagatc
     3961 aattcgaagc cctattagta ttcgctcaat ggggggatca gaggccataa aaagagacta
     4021 gtcattacta gtctcaggta gtggaattgt aggattttaa acgttcctgg agagtaccgg
     4081 tatgtaactc gaagaggtga ttatcatagt cgtaaaagta aattgaagaa gcctcgcctt
     4141 tcacacgaga acgtcctggt tcgacagtta aacctaacat ttggattttt gagacaaacg
     4201 aatctatatc ttgttcgtca attttaaaag caatatgatt gtatgtcttt ggcagtttat
     4261 tcgacgaatt ctgcattact gcaatccata ggtccttaac taagaaaaat ttttctggat
     4321 ataatgaaaa tttttttgtt gactatcgta gatttctttt gcatcaaata aggtatttaa
     4381 caattaagta tttaaaagat aacttttaag ttatctttaa gtaatgctta aagctatgtt
     4441 atact
//
LOCUS       DAYYCW010000275         4106 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.guided.165, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000275 DAYYCW010000000
VERSION     DAYYCW010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.guided.165"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..1534
                     /gene="msr(C)"
                     /locus_tag="ACHAYA_003101"
     CDS             56..1534
                     /gene="msr(C)"
                     /locus_tag="ACHAYA_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8606800.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1581..2027
                     /locus_tag="ACHAYA_003102"
     CDS             1581..2027
                     /locus_tag="ACHAYA_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8606801.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2226..2741)
                     /locus_tag="ACHAYA_003103"
     CDS             complement(2226..2741)
                     /locus_tag="ACHAYA_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8606802.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2858..4060
                     /locus_tag="ACHAYA_003104"
     CDS             2858..4060
                     /locus_tag="ACHAYA_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8606803.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1222 a          700 c          893 g         1291 t
ORIGIN      
        1 gctctttttt agtatcgata attgttgttt tacatgaaat aggagtgtaa aacatatgga
       61 aaatttagca gtaaatataa caaatctaca agtcagcttt ggcaaccagc tagaactatc
      121 tattgattct cttcgtgtct atcagcaaga tcgtataggg atcataggag aaaatggtgt
      181 cggtaaatcg actttgctca aactaatagc cggtgaactt tttcccgatc atgggaaaat
      241 ccaaacagag atcaccttca actacctgcc tcaattaacc tatctcgctg aagcaaagga
      301 cctaaatttg gaattagcca gtcatttcca gttaagactg gaagaaactt cagagcggaa
      361 atggagcgga ggggaagaac gaaagatcga gttgatacgt cttctttctt cttatgaaca
      421 agggatgctt ctagacgagc caacaaccca tctagataga aaaagtattg atcgactgat
      481 tgaagagctt cgttattatt atggtacgct ggtttttgtt agtcatgatc gctattttct
      541 agatgagcta gcatcgaaaa tctgggaagt aaaagacgga gaaatccgag agttttcggg
      601 gaattatagt gcctatctca ctcaaaagga attggagaaa aagactcagc tacgagaagc
      661 agagtcgatc atgaaagaga aaaaacgatt ggaaaaatcg atccaagaaa agaaaaaaca
      721 agcggaaaag ttggaaaaag tgtccagtaa aaagaaaaag caacaaatca gactggatcg
      781 gttgtcttcc tctaaacaaa aagacagtgt acaaaaagcc atccaaaaga atgcgaaaac
      841 attagagaga agactccaaa aaataggaga aacaaccaag ccgcagcaga tgaaacaaat
      901 ccgttttcca gtaccaaaat ctcttgaact ccacagccgc tatcccatca tgggacaaaa
      961 tgtccaattg gaaagaagtg ggagaacatt actggtaaat ggtgattttc agttttcttt
     1021 aggtaaaaaa atcgcgattg tcggtgaaaa tggttcaggt aagacaacct tattagaaca
     1081 tatccgcaaa caaggagaag gaatccttct ctctccgaaa gtaagctttc aagtatatca
     1141 gcaaaagggt tatcaaatga catctgaaga atccatcatt cgttttgtca tgagacaaac
     1201 agagttttca gaatcgcttg tccgtagttt gctgaatcac ttagggtttg ctcaggaaac
     1261 tttgacgaaa ccgttatgta cattaagtgg gggagaagcg acccgtctga cgattgcttt
     1321 gctttttact aagccaagta atgtgttgct gttagatgaa ccgactaatt ttattgatat
     1381 ggcaacgatc gaagctttag agaagctaat gcaaatatat ccgggaacga ttttgtttac
     1441 ttctcatgat tcttactttg tcgagcgtac ggctgatgaa gtttatgaaa taaaagggca
     1501 gaaaataaaa aaagtactta cgagaaattt ttaaatgtgc tacgatgatt ttcgtgacaa
     1561 atttaaagga gtggtacaga ttgcctacaa ataaaaaaga aggaataatt tttacaacaa
     1621 tgatgtgttt tttgatggtc ttggggatga gcgcgtataa tttatggctg catcaagatc
     1681 ttcatttatc aagtttgttg attggtctag tgcctggatt cgtggtggct ttcattttgg
     1741 atgtcttcat catcggtgtg ctggcgaaaa aaattgcctt taaactacca gtaaatccag
     1801 aaagcaaggt ccaaatgatc ttaactattt catgcttgat ggttttagga atggtgacat
     1861 gtatgtcttt attcggtgtg atcatggaac aaggaatacc tgatcaatta ttctctgctt
     1921 atatgaatgc ttggaaaatg aatgtgattg ccgctttacc gttgcagttg atcatcgttg
     1981 gcccgctttc aagaagtgtg ctaaaagcaa ttcaaagtaa tgaatgatgg aacactgtta
     2041 tttgaaaaaa ggccttggca tgctttttgt catagtctct tgaatgcagt tctagaaaaa
     2101 tagtgatccg aacgataggt tttggcctgt cgttcggatc gctttttctt ttccaactgt
     2161 attgctcttt tattgaagat aatttgctct tcaatagata tttctagaaa ggttctgttt
     2221 atcccttact caaaaaactg ctgcatttgt tctcttactt gctgacacca ttggagttcc
     2281 tgctgacaaa gcataattct gcgctggatc acaacataac gtccaaattc ttgtttcttg
     2341 tctagcgaga ggtcattcaa ttttttttga tttttttgca tgattttgtt cagctgttgt
     2401 tctctagtga atagcagtcc agtagctgtt gttttatcga gcgtcgaaaa agcatagatt
     2461 tttgctagaa attcatcttt ttgcgtagga gcgggagttt cctctttgat ccattcctct
     2521 acaagagcac gtccttcatc agtcaagtgg tagatctttt tttgagagtg ctcgtctttg
     2581 tccaaaacgg tcacataacc ctgctggtct aacttggcaa gcgttgtata gatctgactg
     2641 tgatgggctt cccaaaaaat tgtgatcagc tgcttcaatt catacccaga acgtggtttg
     2701 gataaaagca cgcataataa tatataggct aaagtattca taaaaatcct acctctcgtc
     2761 ttattttctt tattatatca cagtaaagtt tccacgtttt acatgtgcat attgacatgt
     2821 aaaataaatc atgtatgatg ctaatagaga gaaagggatg gaaatattgg agaatcaaat
     2881 agatatacaa gaacaaacaa atgaagaaac aaaggggaaa agttcagcgc tagttgtagc
     2941 agctacctgt atgatcgctt ttatgggagt gggtctagta gacccaatct taaaaacgat
     3001 tgctgcacag ttaaacgcta cgccagctga aacgaccctg ttatttacta gttacatgtt
     3061 agtcactggt gtggtgatgc tatttacagg tttcttatct agtcggatcg gtttgaaaaa
     3121 aacattgatg acaggattgt ttatcatcgt tgtattcgct ggtcttggcg gattatctgg
     3181 gaatatcgga atgctgattg ggttacgtgc cggctggggt gtcgggaatg ctttatttgt
     3241 ctctactgct ttagcaacga tcgtcagtat tttagttggt aacacagaaa aagcaatcat
     3301 gatgtatgaa gcagcacttg gctgcggaat ggctgttggc cctctagtcg gtggggtctt
     3361 gggcagtggt tcatggcgtt atccattttt tggggtagca tttttgatgt tttgcggttt
     3421 tatagcccta gcggtacttt tacctgaaac tgagaaacca aagagaaaag taggcctttg
     3481 ggaaggtgtc aaagcgttaa gcaatcataa acttcgttca gttggactaa tcgcattgtt
     3541 atataatttc ggattcttta ctttacttgc gtatgcgcca tttttattga ttggctattc
     3601 tgaattagaa gtcggttttg tattttttgg ctggggtgtg cttctggcta ttgcttcgat
     3661 tttcgttgca ccggctcttg agcggaagtt tacgacttat cggacaattt tagcagcatt
     3721 tgtcttattc attatttgtt tgctgttatt aggagctgga acttctatgc cagtccttgt
     3781 accgatcagc attgtgttag caggattttt ccaaggaatt atcaatacac tgttgacgac
     3841 gattgctatg gaaataccag gtctgcaacg aaacgttgct tcgtccagct atagttttgt
     3901 ccgctttttc ggtggagctt tagcaccatt tattgcaggt gtgattggtg aacatattgg
     3961 tgaaagctat gcgttttatt ttgcaggtgc cattgttctt atcagtgtat gttgtatcat
     4021 ttatcaccgt aattattttg ctacaaatga agaagaataa gaaatcagag gttgtgaaaa
     4081 agctgtcctg catcaagtca taagaa
//
LOCUS       DAYYCW010000276         3619 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.guided.178, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000276 DAYYCW010000000
VERSION     DAYYCW010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.guided.178"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..878
                     /gene="erm(B)"
                     /locus_tag="ACHAYA_003105"
     CDS             141..878
                     /gene="erm(B)"
                     /locus_tag="ACHAYA_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8606804.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1141..2004)
                     /locus_tag="ACHAYA_003106"
     CDS             complement(1141..2004)
                     /locus_tag="ACHAYA_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8606805.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2006..2278)
                     /locus_tag="ACHAYA_003107"
     CDS             complement(2006..2278)
                     /locus_tag="ACHAYA_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8606806.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2295..2504)
                     /locus_tag="ACHAYA_003108"
     CDS             complement(2295..2504)
                     /locus_tag="ACHAYA_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8606807.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2582..3478)
                     /locus_tag="ACHAYA_003109"
     CDS             complement(2582..3478)
                     /locus_tag="ACHAYA_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8606808.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1103 a          644 c          554 g         1318 t
ORIGIN      
        1 ttacgcagat aaataaatac gttagattaa ttcctaccag tgactaatct tatgactttt
       61 taaacagata actaaaatta caaacaaatc gtttaacttc tgtatttatt tatagatgta
      121 atcacttcag gagtgattac atgaacaaaa atataaaata ttctcaaaac tttttaacga
      181 gtgaaaaagt actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt
      241 acgaaattgg aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg
      301 taacgtctat tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga
      361 acactcgtgt cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga
      421 ggtataaaat tgttgggaat attccttacc atttaagcac acaaattatt aaaaaagtgg
      481 tttttgaaag ccatgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta
      541 ccttggatat tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat
      601 tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa
      661 aacttacccg ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg
      721 tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag
      781 caatgaaaca cgccaaagta aacaatttaa gtaccgttac ttatgagcaa gtattgtcta
      841 tttttaatag ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttgtaaa
      901 tttggaaagt tacacgttac taaagggaat gtagataaat tattaggtat agggcacctc
      961 taataactac caattaattc acctcaaaat gaaattagaa aaaaacagcg aaatttttaa
     1021 atctatttct tatcgataca aattcctcgt aggcgctcgg gaccccaaca tgaggaaatg
     1081 tttttataaa aaaagagtag ttaccgtttt ttggtaacta ctcttttcct tttttaaggt
     1141 ttaaatacct ggaagtttag gtgttttggg tattggtggt gtaggtagct gcaagctttc
     1201 cattttttgt tgaattgctt taaattcagg ggtttcttgg tgttgatttt gaaccatttt
     1261 ttgctctatt ctctccaaag tcggttgaat ttctttccct gatactttac gatttaattc
     1321 tctttctaag gtctctttcg gactaatgga aggcgtttct aagcttgaat agagtttcac
     1381 tccttctcta ttgtaaagcc ttatatcgct aaataagccc gttttatgaa gggtttctaa
     1441 attggtcggt aagtttttga caacaatatc atgcgcttgt tttggtgttg ccctagctgt
     1501 cattggatca tctgcataca tggtttcata tcgttcaatc gttcctaagt atgattcaat
     1561 tttcggtact gccatgacgt atatttttgt ttcataacct ttagattgaa gcattgtggc
     1621 tgtttgaatg ggaacgtctg ttgttcgtcc tgttccttca atcactaaat tatagccttg
     1681 atcgcttaaa cggctaataa gtgcttctgt catgcgattc gaataagggg taacgtgttt
     1741 tactacgtct ttttcataga gtttcactaa ttcgtcaaaa tttgggtgct gttgtttaaa
     1801 ggtatcatta tcaatgacaa taacattccc ttgtgtttct tcaaaaattg ctgatcgcaa
     1861 actggttttc cctgaccctg gttgcccacc aagtaaaaaa gcggttggcg attcaaccgc
     1921 tagtctattt ttagttagtc gttcaacatt cttttctaaa cgatcttcaa attgcttttc
     1981 ggtgaagtca gtaatattcg ccatgttaag ccactttctc tttattcaaa atttgttttg
     2041 aatattgatt cattgaccgc caatactcat gaatagcttg taattcttca aatggttgtt
     2101 gaaataaatc aacttcttgt gccacttcta attgatttaa aagattcact tctagtaaac
     2161 gagtattttt agtatctagt tcatgattca aaacataatt taatacccga ttataaacac
     2221 ttgccgataa ctcattaatg atttcaattt caaatgtttt ttcatacgta actgccattt
     2281 cattttcact ccttttaaag tttgtcaggt aaatatttcc gtatatattc ttctaaggct
     2341 tgatccataa cttcttttac gtttccgcca ttctttgctg tttcgatttt tatgatatgg
     2401 tgcaagtcgg cacgaacacg aaccgtctta tcccccatta tatctttttt tgcgctgatt
     2461 ggcgtatcat ttcgttttgc tttctgtgcg cctaaatttc ccacgatcgc tcacttcttt
     2521 ctatttcttc ttactattta ttttatcatc acctatcaca aatcacaagt gatttgtgat
     2581 tctattcttt ttcattttct aattgaatga ttcgctcaat catttcaaaa aatacgttct
     2641 catacataga caaaactttt ttgtcatagc ctttgtgttc tgtaatgcca tttttagacc
     2701 aagtacttac tttattactt cgcttgataa tatttcggaa aaccaaatta tcctctttat
     2761 gttctttgta cagttcttcc aggtttgatt ttatcgttgc gctgtccgta tcaactaaat
     2821 aaggaacaaa accaatcata tctagtccag ggttaaactg ttcttgcaaa tcaatcaaat
     2881 aggaaatata gttttgaatg ttgtttgtac tttcttcttc tgcttgtaaa gggatcataa
     2941 cgtaatcact cgccacgatt gcattatttg tataaacgct tggcgttggt acagtatcaa
     3001 taataataag atcatagtca ctttttaaag gtgctaaaag agtagcaagc aatctacttt
     3061 cattttcaaa cgtccatgag cgagttaatt ttggcagtaa catcaaatca aacgtgccag
     3121 ggatcaagtc taaattatca gtcaaatgaa taatagaaga agccaagttt ccatttttca
     3181 agccttcata aaaattgaca cgtggcaatt ctacctcaaa agtttttgct aagtcttttg
     3241 tcaatgttgc ctgtaagtcc ttatcgatca ttaaaacttt taaattcaat ttgtctgtca
     3301 agtaagcaaa catagtcgat aatttggact ttccaacacc acctttaaag taattattca
     3361 agataataat tgcttcattt ttattgttta aaatacgtct taattcttca agtattttta
     3421 gtttttcctt ctccataact acgccccatt ctttttttgt atagtaatat tgtatcatta
     3481 agaatcacaa atcacaagtg atttgtgatt aatcacttgt gatttgtgat aagtgatttg
     3541 tgattaatat ataaaagccc tctttaaagg gcttttatag gttctgttgc aaagttttaa
     3601 ataaagaata aaatccctt
//
LOCUS       DAYYCW010000277         2676 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.guided.196, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000277 DAYYCW010000000
VERSION     DAYYCW010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.guided.196"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..399
                     /locus_tag="ACHAYA_003110"
     CDS             <1..399
                     /locus_tag="ACHAYA_003110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8606809.1"
                     /translation="SLNGLEKLREIFKTAVFNSNQSVLLTVSPQLLNNPRFLAMQIEQ
                     MYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMVLSNVWLNPLVEMTDKEGMEKR
                     CNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            506..1744
                     /gene="mef(H)"
                     /locus_tag="ACHAYA_003111"
                     /gene_synonym="mefH"
     CDS             506..1744
                     /gene="mef(H)"
                     /locus_tag="ACHAYA_003111"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8606810.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            1971..2390
                     /locus_tag="ACHAYA_003112"
     CDS             1971..2390
                     /locus_tag="ACHAYA_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8606811.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            2395..2634
                     /locus_tag="ACHAYA_003113"
     CDS             2395..2634
                     /locus_tag="ACHAYA_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8606812.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT          870 a          371 c          494 g          941 t
ORIGIN      
        1 agtttaaacg gtttagaaaa gttaagagaa atatttaaga cagcggtatt caattctaac
       61 caaagcgttt tattgacagt ttctcctcaa ctattaaaca atcctcgttt tcttgctatg
      121 caaatagaac aaatgtatga aattgtagta cctgaatttg ttgaacccgt tttagaacaa
      181 ggtatcaagg ataagtcttt ggagattgaa aattcacatg aaattgcaga agttatcatg
      241 gtattaagta atgtatggct caacccatta gttgaaatga cggataaaga gggtatggaa
      301 aaacgttgca atacattcaa tgccctttta aatggaatag gcatagacac attattagac
      361 gaagaaacaa tttctgcatt tatacagttt tgtaaataac tgtatttgtt atccctatac
      421 gatagactgc tctataaaag agcagtttat ttaaaccatt atataaaccg acggtctgtt
      481 tgattaaata aaaaggagag attttatgca agcagaaaaa aaacttttta atcgtgattt
      541 tatacttgtt gttataggac agataatttc tttatttgga aacgctatcc tacgatttgc
      601 attaccgtac tatttgttaa aagaaactgg ttcatcagct ttgtttggta ttgctacagc
      661 ctgctctttt ttacctatgg ttattttgtc gctggtaggt ggtattattg ctgacaggac
      721 aaataaaaga aacattatgg tcatactaga ttttgtaaca gcaatcataa ttttagtatt
      781 ttatttgtca ttgggaaaaa ttcctatcat acctttgttt attgttgctt tgatgttact
      841 ttatggtatt tctggtgcat atcaaccagc ggtacaagca agtattcctc tattggtatc
      901 atcagataaa cttaccgtcg gtaatgctat aataaatcaa gtaaatactt tagcaaactt
      961 tatcggacca gctataagcg gagtagtttt tcaattttat ggtatcaatc ccattttaat
     1021 tgttagtatt atatgtttta ctttttcagc cattatggaa atttttatcc atattccaca
     1081 ccaaaaaaga cctcgtgaag taggtgtaat tgcagttgct aaaaacgatt taaaagatag
     1141 ttataccttt ttgaaaaagg agaaacccat ttttatatcc gtaatctttt tggtttgcat
     1201 tttcaatttg gttttaagtg ctgttttaat tgttggtctg cctatcttaa tttctgaagt
     1261 tttaaaaatg ccagatacac tatatggttt ttcacaatca gcattatcat taggtggttt
     1321 atgtggtggt attttaacag ctcttgttgc ttcaaagtta aaactaaaaa acctttatac
     1381 cttgttactt atttgttctg tttctgtttt acttatggga atttcgctat ttttagaatt
     1441 gcctgcaata gtttcctact gggtagttac agtgatgtgc tttgtggcta tgggtgtatc
     1501 tactatgttt gttgttcaaa tttacactct tgttcagaca caaacaccac cgcaattagt
     1561 aggaaagata atggctacgc tggtagcagt tgctatgtgc ggtcaaccta ttggacaaat
     1621 ggtttacggt attttattcg atgtatttat acaagaaaca tgggttgtta tgtttgtagc
     1681 ttctattgct tcaatcatga tttcaatata ttcaaaaaga atttttcaaa agttagagat
     1741 ataatacaag gggttaaata gtatgttagg ctaaaaacaa tatataaaag aaaatttgaa
     1801 atctgctaaa aggatagtgt tataaaagaa agagtaatat tgcagtattt aaggctcgca
     1861 caaatatatg ttttgtgtgg gcttttttct taccattttt ctgtatttct aaagggtata
     1921 aacggtattc cgtttatata tttcagcatt agcagaaagg gggtgagatt atgaggacat
     1981 cttctttcga gcatatcgtc agattacagt tcaattcttt aatgctgatt gtcattaaga
     2041 acaaagtgaa aagtcgaaac agacagcttg caagacattc taaacgtgaa gtattgtttt
     2101 gtgaaatagc cgaaacgaaa caagttgaac gtggaacgat tgatacctgt ttttatgatt
     2161 gtgtatcatt taaagtcttg cactttacaa tttatgtatc tgatgaaaca cttggtacag
     2221 ttttacatag actgtcagaa aaacagcgta gtgcgatttt attacgttat tttcaaggaa
     2281 tgaacgacag agaaatttca gaactgtacc atgtatcacg ttctgctatt tgcagtagaa
     2341 gaactagagg actaaggaag ttaaaaatgc ttttgagcga aaggaagtaa agaaatgagt
     2401 aaagattatg gctatccctc ttttgaattg atttgcaaag cgtcaagcgg agatgaaatg
     2461 gcagttaaag agattttgaa gttttatgac ggttatattt ctaaattatg cttgcgtcct
     2521 ttttaccact ctgaaagtgg taaaatcatt atgcaggttg atgaagaact aaaaggagaa
     2581 attcatacag acatgatgaa agcaatattg aaatttgaaa tcggagtgaa gtaatcaact
     2641 gtataaaaaa atcgggagat acacagtttc gtgcta
//
LOCUS       DAYYCW010000278          981 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5415
            SAMEA115707704-rid22593663.guided.250, whole genome shotgun
            sequence.
ACCESSION   DAYYCW010000278 DAYYCW010000000
VERSION     DAYYCW010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707704
            Sequence Read Archive: ERR13197432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290146.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290146.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:45:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,053
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 33 of 82
            Pseudo Genes (incomplete)         :: 38 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707704-rid22593663.guided.250"
                     /isolate="VRE5415"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            368..916
                     /gene="aac(6')"
                     /locus_tag="ACHAYA_003114"
     CDS             368..916
                     /gene="aac(6')"
                     /locus_tag="ACHAYA_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8606813.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          346 a          142 c          193 g          300 t
ORIGIN      
        1 caataatgaa tttttgttcc tcttagtatt tctaccgatt ccctttgctt ttttgtaatt
       61 atctaaaaat tctaaaaatt aagattatta tatgaaaaca ggttttattt gtcaattcat
      121 aattggtttt ttttaaagca aaagataatc tatatcatga tgaacaaaca gagttttttc
      181 ctccaattga tggctttcct gatctttcct attttgaata aagtgtttct gtataatttt
      241 ttattttata agtaagataa ataatgaaag aatagagata ctgattggta gctaaagatt
      301 tttgagcgag aacatttcgt tttttcaaaa ggactgttag aataagcaag aacaaggggg
      361 aagacgtatg ataatcagtg aatttgaccg taataatcca gtattgaaag atcagctttc
      421 tgatttactg agactgactt ggccggaaga atatggagac agctcggcag aagaagtaga
      481 agaaatgatg aatccagaac gaatcgcggt agcagcggta gaccaagatg agttagtagg
      541 atttattggt gcaatccctc aatacggtat cacaggttgg gaattgcatc cattagttgt
      601 agaaagctcc cgacgaaaga accaaatagg tactcgatta gtcaattact tagaaaaaga
      661 agtagcttcc agaggaggaa tcacgattta tttaggtacg gatgatttag accatggaac
      721 aacgttaagt caaacggacc tgtatgagca tacatttgat aaagtggctt ccatccagaa
      781 ccttcgtgaa catccgtatg aattctatga aaaattaggt tataaaatcg taggtgtctt
      841 accaaatgca aatggctggg acaaaccgga tatttggatg gcaaaaacga ttattcctcg
      901 accaaattct caataataaa aagaggttgt gacagaagta atcagcttca agaaataaga
      961 aggaatttac gaaaattact t
//
